LOCUS       DAZULY010000001        43596 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000001 DAZULY010000000
VERSION     DAZULY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.001"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..1531
                     /gene="cls"
                     /locus_tag="ACG81A_000001"
     CDS             83..1531
                     /gene="cls"
                     /locus_tag="ACG81A_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS5013413.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            1868..2725
                     /locus_tag="ACG81A_000002"
     CDS             1868..2725
                     /locus_tag="ACG81A_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFS5013414.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            2840..3760
                     /gene="pstC"
                     /locus_tag="ACG81A_000003"
     CDS             2840..3760
                     /gene="pstC"
                     /locus_tag="ACG81A_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFS5013415.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            3760..4644
                     /gene="pstA"
                     /locus_tag="ACG81A_000004"
     CDS             3760..4644
                     /gene="pstA"
                     /locus_tag="ACG81A_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFS5013416.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            4659..5465
                     /gene="pstB"
                     /locus_tag="ACG81A_000005"
     CDS             4659..5465
                     /gene="pstB"
                     /locus_tag="ACG81A_000005"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS5013417.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            5481..6239
                     /gene="pstB"
                     /locus_tag="ACG81A_000006"
     CDS             5481..6239
                     /gene="pstB"
                     /locus_tag="ACG81A_000006"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS5013418.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            6252..6929
                     /gene="phoU"
                     /locus_tag="ACG81A_000007"
     CDS             6252..6929
                     /gene="phoU"
                     /locus_tag="ACG81A_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFS5013419.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            7506..8198
                     /locus_tag="ACG81A_000008"
     CDS             7506..8198
                     /locus_tag="ACG81A_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS5013420.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            8195..8962
                     /locus_tag="ACG81A_000009"
     CDS             8195..8962
                     /locus_tag="ACG81A_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFS5013421.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            9062..10630
                     /gene="liaX"
                     /locus_tag="ACG81A_000010"
     CDS             9062..10630
                     /gene="liaX"
                     /locus_tag="ACG81A_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFS5013422.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            10927..11247
                     /locus_tag="ACG81A_000011"
     CDS             10927..11247
                     /locus_tag="ACG81A_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFS5013423.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            11247..11597
                     /locus_tag="ACG81A_000012"
     CDS             11247..11597
                     /locus_tag="ACG81A_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFS5013424.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            11764..12702
                     /gene="hprK"
                     /locus_tag="ACG81A_000013"
     CDS             11764..12702
                     /gene="hprK"
                     /locus_tag="ACG81A_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFS5013425.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            12717..13547
                     /gene="lgt"
                     /locus_tag="ACG81A_000014"
     CDS             12717..13547
                     /gene="lgt"
                     /locus_tag="ACG81A_000014"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS5013426.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            13591..14616
                     /locus_tag="ACG81A_000015"
     CDS             13591..14616
                     /locus_tag="ACG81A_000015"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS5013427.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            14631..15569
                     /gene="galU"
                     /locus_tag="ACG81A_000016"
     CDS             14631..15569
                     /gene="galU"
                     /locus_tag="ACG81A_000016"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS5013428.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            15694..16134
                     /locus_tag="ACG81A_000017"
     CDS             15694..16134
                     /locus_tag="ACG81A_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFS5013429.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            16137..16739
                     /locus_tag="ACG81A_000018"
     CDS             16137..16739
                     /locus_tag="ACG81A_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS5013430.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(16798..17904)
                     /locus_tag="ACG81A_000019"
     CDS             complement(16798..17904)
                     /locus_tag="ACG81A_000019"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFS5013431.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            18114..19115
                     /gene="ccpA"
                     /locus_tag="ACG81A_000020"
     CDS             18114..19115
                     /gene="ccpA"
                     /locus_tag="ACG81A_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFS5013432.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(19452..21797)
                     /locus_tag="ACG81A_000021"
     CDS             complement(19452..21797)
                     /locus_tag="ACG81A_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFS5013433.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            22076..22978
                     /locus_tag="ACG81A_000022"
     CDS             22076..22978
                     /locus_tag="ACG81A_000022"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFS5013434.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            23191..24966
                     /locus_tag="ACG81A_000023"
     CDS             23191..24966
                     /locus_tag="ACG81A_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFS5013435.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(25032..26618)
                     /locus_tag="ACG81A_000024"
     CDS             complement(25032..26618)
                     /locus_tag="ACG81A_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFS5013436.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            26869..27093
                     /locus_tag="ACG81A_000025"
     CDS             26869..27093
                     /locus_tag="ACG81A_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFS5013437.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            27235..28986
                     /locus_tag="ACG81A_000026"
     CDS             27235..28986
                     /locus_tag="ACG81A_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFS5013438.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            28986..30770
                     /locus_tag="ACG81A_000027"
     CDS             28986..30770
                     /locus_tag="ACG81A_000027"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5013439.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            31096..31947
                     /gene="licT"
                     /locus_tag="ACG81A_000028"
     CDS             31096..31947
                     /gene="licT"
                     /locus_tag="ACG81A_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS5013440.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            32040..33926
                     /locus_tag="ACG81A_000029"
                     /pseudo
     CDS             32040..33926
                     /locus_tag="ACG81A_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
     gene            complement(33966..34868)
                     /locus_tag="ACG81A_000030"
     CDS             complement(33966..34868)
                     /locus_tag="ACG81A_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5013441.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            35099..36502
                     /locus_tag="ACG81A_000031"
     CDS             35099..36502
                     /locus_tag="ACG81A_000031"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS5013442.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            36502..37935
                     /locus_tag="ACG81A_000032"
     CDS             36502..37935
                     /locus_tag="ACG81A_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS5013443.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            38080..39006
                     /gene="trxB"
                     /locus_tag="ACG81A_000033"
     CDS             38080..39006
                     /gene="trxB"
                     /locus_tag="ACG81A_000033"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFS5013444.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            39220..40947
                     /locus_tag="ACG81A_000034"
     CDS             39220..40947
                     /locus_tag="ACG81A_000034"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFS5013445.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            40962..41312
                     /locus_tag="ACG81A_000035"
     CDS             40962..41312
                     /locus_tag="ACG81A_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS5013446.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(41404..42264)
                     /locus_tag="ACG81A_000036"
     CDS             complement(41404..42264)
                     /locus_tag="ACG81A_000036"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFS5013447.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            42356..42784
                     /locus_tag="ACG81A_000037"
     CDS             42356..42784
                     /locus_tag="ACG81A_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS5013448.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            42946..43122
                     /gene="rpsU"
                     /locus_tag="ACG81A_000038"
     CDS             42946..43122
                     /gene="rpsU"
                     /locus_tag="ACG81A_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFS5013449.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            43149..43595
                     /locus_tag="ACG81A_000039"
     CDS             43149..43595
                     /locus_tag="ACG81A_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFS5013450.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT        14032 a         7640 c         9300 g        12624 t
ORIGIN      
        1 gaatagaagg aagcggcttt tttcccttta ctttaaaaaa caacttgggt attcttaagt
       61 tggtagaaga agaggtgaag ccgtgaaaat tatcgttgac aattttttta cgatcctttt
      121 gattatgaat attttgcttt cctttatcat cgtttttcga gaaagaaagg aaactgctca
      181 aacatgggca tgggtgctcg ttttgatgtt cattccagtt gtaggattta ttttatatat
      241 ttttttagga agagggattt ccaaagagaa aatctttgat ttgaagatcc aagggaaaat
      301 cggtaagaac ctggaaattg aagaagacaa acaggcattg atgcgagggc tttatcctca
      361 tccgcctact ggacaagtag atgtgaaaca attgatctat atgctcacgg tatttgaaag
      421 tacgctctat acgactaaaa atgaaatcac tttatttaca gatggtcgtg aaaaattcga
      481 tgcgctgatt gaagatatca agcaggcaga agaccatatc cacttccagt attatattta
      541 tcgcagtgat gcattaggtg aagaagttcg agatgcattg acagaggcgg caagacgagg
      601 ggtaaaagtc agagtccttc ttgatgcatg gggttccaca caagtttctt ccagcttttt
      661 tcagaatttg aaaaaagcag gcggagaagt cgcatttttc tttcctctat ttgttccgta
      721 tattaatcct cggatcaatt atcgcaatca tcgtaaaatc gtagttattg atgggaaaat
      781 cggatataca ggtgggttca atgttggaaa tgaatatcta ggattggtaa agaaatttgg
      841 ttattggcga gacaatcatt tgcgtattta cggagaagcg gtgtatacct tgcaaaaccg
      901 ttttttgatg gactggaatt ctcagcatac aaaagaagta ggctattctc ccaaattttt
      961 tccgtccatt cactctactg gtgatatagc tgttcaaatc gtgacaagcg gaccagatac
     1021 cgaacatgaa caaatcaaaa tgacttattt gaaaatgatt tcgttagcca aacgggagat
     1081 attgatccaa acgccttatt acataccgga tggatcgatt catgaagcac tgaagttggc
     1141 tcttctttca ggcgtaaaag ttcatatcca aatacctaac aaaccagatc atcttctcgt
     1201 ttactgggca acgtattctt tcgctgcaga gctaatcgag tatggagcaa ggatcgaaac
     1261 atatgagaat ggatttattc atgcaaagac gatgattatc gatggtggca ttgtttcagt
     1321 tggctcagca aatatcgatg ttcgttcatt tcgcttggac tttgaggtca atacactggt
     1381 ttatgatgaa cgaatggctt cacgtgtaag aaaagctttt tttgaagact cgaaaatttc
     1441 tacgcatctc acaaaagaaa tgtatgaaaa ccgaggaata atcatcaaga tgaaagaagg
     1501 attagcaaga ttgatttcgc cgctccttta aaacataaaa aggacatgag aaccatcaac
     1561 agatggagat ctcgtgtcct tttctaacgg ggccatttct gagagtacgt cagaaatgga
     1621 gaaagcgtct cgcatgagcg aaattaactt tggggtatct cttgtttttt ggggaaagca
     1681 agaaataagc taatatatgc tattaaaaga gacacatttc aaagccagta tagcatagaa
     1741 acttttaatg agatagtttt ttttacaatt atgagaattt attttgttat ttaatgattt
     1801 tgattcgttt tcatcaagta atgaaaatga taaaataaac atactttagg attaaaggag
     1861 atttctaatg aaaaaactta aattattctt accaataata ggattagcta ttcttttatc
     1921 aggctgttca aagtggattg atcgaggaga atccattacg gctgttggct cttctgcttt
     1981 acagccttta gtagaaacag ttgctgaaac ttaccagaca gaaaatcctg gaaaattcat
     2041 caatgtacaa gggggcggaa gcggaactgg tttgtctcaa gtacagtcag gagcagtcga
     2101 catcggaaac tctgatttgt ttgcggaaga aaaatcgggg atcgatgcct cagcattagt
     2161 cgatcatcga gtagcagttg ttgggatcac tcctattgtg aacaaggata ttggcgtaaa
     2221 agacatcagt atggaaaacc tgaaaaaact atttacaggc gagatcaaaa actggagtga
     2281 agtcggtgga aaagatcagc cagtagttat attgaaccgc gcttcaggaa gcggaacccg
     2341 tagcacgttt gaaaaatggg tattggacgg agaaacagct gttcaggcac aagaacaaga
     2401 ttcaagcgga atggtgcgcc agatcgttgc cgacacaccc ggtgcaatca gctatgttgc
     2461 tttttcttat gtttccgatg atgtagatac gttgaatgtt aacggtgtag aaccgacaga
     2521 tgaaaatgtg acaacgaaca aatggacgat ttgggcatat gagcatatgt acacaagaga
     2581 tcatccaaaa aaactgacaa aagaattttt agactatatc ttatctgatg aagtacaaga
     2641 taacatcgtt ggagaactag gttatatccc tgtttctcat atgaaagtcg aacgtgattg
     2701 gcaaggaaat atcgttaaag agtaatcttt acacaaaatt tacaaactta gctaaacttt
     2761 tacatgttat taaaaccaaa ttgatgttca atttgtacac tgagtgagta tgttaaacct
     2821 gattaagggg gagtcatctg tggaagatgt gaaaaaagta ttattaacaa aatcaaaacg
     2881 ttcaaaaatg gaacagcgtg gaaaattcat cagtttttta tgtatcgcgc tgatcgtgtt
     2941 ggttgtatta tctatttttt attttgtagc tagtaaagga ttggccacct tttttactga
     3001 taagatcaat gtatttgatt ttctgtttgg tacagattgg aatccaagcc aaatcggagc
     3061 aaatgggaaa ccaatggtag gggcattgcc tatgatcaca ggttcgttta ttgtgacctt
     3121 tttatctgct atcgttgcca cgccatttgc cattggtgca gcagtattca tgacggagat
     3181 ctctcctaaa aacggaacga agatcttaca gccggtcatc gagctgcttg taggtattcc
     3241 ttctgtcgtt tatggtttta tcggtttatc agttatcgtg ccttttgtca gaagtatttt
     3301 tggcgggact gggtttggta tcttagctgg tacatttgtg ttgttcgtta tgatcttgcc
     3361 gactgtcaca acgatgactg ttgatgcatt aaaggcagtg ccacggcact atcgggaagc
     3421 ttcattagcg cttggtgcca caagatggca aacaatctac aaagtcgtgc tacgagcagc
     3481 cgtgcccggg attttaacag cagtcgtttt tggtatggct cgtgcgtttg gtgaagcctt
     3541 agctatccaa atggtcatcg gtaatgcagc attgatgcca acaggtctta ctactcctgc
     3601 ttctacattg acgtccatct taacaatggg aatcggaaat acaatcatgg gaacagttga
     3661 aaataatgtg ttatggtcat tagcgctgat cttgctattg atgtcattat tctttaatat
     3721 cgtgatccgt atgattggga agaaaggaga gctgaaataa tgaacgcaaa acgctcagat
     3781 aaaattgcta caggtgtttt atatatagtt tcggggatca tcgtattgat tttagcagct
     3841 ttactacttt atatcttagt tcggggaata ccgcacattt catggtcctt cttgaccgaa
     3901 ccttccaaag cctaccaaga aggcggtgga atcgggatcc agctatttaa ctcgatttat
     3961 ttgcttttga tcacgatgat catcagtttt cctatttctt taggtgcggg tatctatctt
     4021 tcagaatatg ctggaaaaaa ctggctaaca gatgtgattc gcacgtcgat tgaaatcttg
     4081 agctcgttgc cttctgtcgt tgtcggttta ttcggtttct tggttttcgt tattcaattc
     4141 cagtatggtt tctcgattat ttctggtgca ttggctttga ctgtattcaa cttgcctctt
     4201 ttgactagaa cagttgagga gtctttacaa gcggtccatt acacacaacg agaagcagga
     4261 ttggccttag gtttgtcacg ctgggaaaca gtgataaaag tggtggttcc agaagcgaca
     4321 ccagggattt tgactggtgt gatcctaagt tctggaagaa tttttggtga agcagctgca
     4381 ttgatctata cggctggaca aagtgcacct gcgttagatt ttacgaactg gaatccatta
     4441 agtgtttcaa gcccgatcag cattttccgt caagctgaaa cattagctgt ccatatttgg
     4501 aagatcaata cagaaggcac gatgcctgat ggtgtttctg tttcggctgg cgcttctgcc
     4561 gtgttgatta ttgctgtttt actgttcaac ttcggcgctc gtgcgattgg caaacgatta
     4621 taccgaaaaa tgacttcagc ctaaggagaa gaagcaaaat gaaaaaatat aactggaatg
     4681 aaacaaatgt catcaccatg ccaaaagaga aagaagtagc actatatacg gatgatctac
     4741 atgtctggta tggcgacaat gaagcaatca aaggagtgga tctggaattt gaaaaaaata
     4801 agatcactgc actgattggt ccttccggct gcggaaaatc tacgtatctt cgttcgttga
     4861 atcgaatgaa tgatgggatc gctaatacga aaatcactgg gaaaatcatg tatcaaggcg
     4921 tagatattaa tgcgcctcaa gtggatgttt atgagatgcg caaacggatt ggcatggttt
     4981 ttcaacgtcc taatccgttc agcaagtcga tttatgacaa tattacattt gctttgaaac
     5041 agcacggcga aaaaaacaaa aagaaattag atgaaatcgt tgaaacaagc ttgaaacaag
     5101 ctgccttgtg ggaccaagta aaagacagct tggacaaaag tgccttggct ttatctggcg
     5161 ggcaacagca acgtttatgt attgctcgtg cgatcgcgat gaaacctgat atcttgctgc
     5221 tagacgagcc ggcaagtgct ttggacccta tttcgacagg aacggttgaa gaaacacttg
     5281 taaacttgaa agaagactat acgatcatca tcgtgacgca taacatgcaa caagcagctc
     5341 gtatcagcga ctatactgcc tttttctatt taggaaaagt aatggaatat gacaaaacta
     5401 acaagatatt taccagacca aaaattcagg caaccgaaga ttatgtgtcc ggtcattttg
     5461 gttaagacgg aggcctcaac atgacaaaag agattatttc atctaaagat cttcatctgt
     5521 attatggaaa gaaagaagca ctaaaaggga tcgatctaag cttcaatcaa ggggaaatca
     5581 ctgcgatgat cggtccttct ggttgcggaa aatccactta tctgcgttct ttgaaccgga
     5641 tgaatgacct gattccgaat gtaacgatca ccggaagtgt tttatataaa gacaaagaca
     5701 tctatggtcc aaaaacagat gttgttgaac taagaaaaga aatcggcatg gtattccagc
     5761 agccaaaccc atttcctttt tctatctatg aaaatgtcat ctatggattg aaattaaaag
     5821 gggaaaaaga taaaaaagtc cttgatcaag tagttgaaga aagcctgaag gcagcgtctg
     5881 tttgggatga tgtgaaagac aaattgcata aaagtgcttt atctttatcc ggtgggcagc
     5941 agcagcgtgt atgtatcgca cgtgttttgg cagttaatcc ggaaatcatc ttattggatg
     6001 aaccaacaag tgcgcttgat cctgtttcta ctgggaaaat cgaaagtatg ctgttagaat
     6061 taagagagcg gtatacgatg atcatcgtga cccataatat gtctcaagct tcacgtattt
     6121 cagataagac ggcattcttt ttagatggtc acttgatcga atacaatgat acgaagaaga
     6181 tattcatgaa tccagaaaaa caagagacag aagattatat ttcaggtcga tttggataag
     6241 gaggaaacgg aatgctacgt acacaatttg aagaagaatt gctgaatctc cataatcagt
     6301 tctacgaaat ggggatgatg gtgagcagcg ccgtgcacaa gtcagttcgt gcatacatca
     6361 aacacgataa aatcttagcc caagaagtta tcgaaaacga tataaatatc aataacatgg
     6421 aaacacgctt ggagaagaaa agttttgaaa tgatcgcatt gcaacagcca gttacaactg
     6481 atttgcgtat gatcattacg gtcatgaaag ctagctcgga tttagaacgt atggccgatc
     6541 atgcggtgtc tgttgctaaa tccacgattc gcgtaaaagg gcagacgaga ataccggaaa
     6601 tcgaaaaaga gatctccgat atgtccgatt atgtgaaaaa aatggtggac aacgtcctag
     6661 ttgcttatgt caaaacagac gaaaaagacg cacgtacgat cgccaatatg gatcaaagag
     6721 tcaatgacta tttcaatcga atctataatg caacaatcaa aaatatgcag gaaaatcctg
     6781 agacagtcat cagtggaaca gactacttga atgttgccag ttatctagaa cggatcggag
     6841 actatgtgac aaacatctgt gaatggatcg tctacttggc tacagggaaa atcactgagt
     6901 taaatacgaa ccacaatgag attttctaaa ctgaagctgt gattatgccg tttgtttagg
     6961 aaaagaggtt gtgaaatcag cggttgtgaa aaagctgttc tgcatcaagt aataagaaca
     7021 gtcaaacaaa aaatagctcc tcatattttt cgtttggctg gacttattac cgcagatgca
     7081 agcttttgaa caccgtttat tcgggaaagg gtttgtgaca aaacatgtca caaacccttt
     7141 tcatcatttc atagaaagga taaagaatgc aaaaaaaaat tagcatcatc actatatttt
     7201 ttatttgtgt gcttacttca tgggttattc agcatcaagc cgctttatat gaagtacctg
     7261 tcatcgagat agaaaaagta cgaataaaag aggatcatca attgattaca ggaaaactga
     7321 tgaacacgga tcaaaaagga gaatcgattt atttatcggt tccttatcaa aaaaatcaaa
     7381 tagaaacagc aaatataaaa gcgagacaaa tgtatctagt ccaaatgcat ggagatgaat
     7441 ggcagctagt agcaaaaaaa aatgatggct ggcttttctt cttcacaagt atcttcattg
     7501 gcgccatgtt gcttattggt ggaaaacagg gtctatttgc tttaggaagt ctcatcatca
     7561 atatcgtttt acttttactt ttgttgctca tctttactaa aacagaaggg atttctctat
     7621 taaatatatc tatcctgttc gtcgttatag ggatgattat tagcatattg gctttagatg
     7681 gatggaatag aagcagtata atcaaaattt cagctgcagt tacgagtgtt tttttagcat
     7741 tttttatttg tcttttgaca atgaatcatt ggaaagacca aggactgcgc tatgaggaac
     7801 tgaattatct gacacgaccg tatcgttctg tttttctttc tagcgtactg attggaacag
     7861 ctggaggaac actcgatacc gttatcacag tcattgcgac attagaagaa ttgactaagc
     7921 gacaaccaga aataacagcg aaacagttat ggctttcagg aaggcaagtg ggtcgagatg
     7981 tgattggctc gatgaccaat atccttttgt tcgtctatat cagtgggtca gttccatcgc
     8041 ttcttttata tttaggaaat caatggtcgt tcaaagaaac gatagaacag catttatcat
     8101 tggaattttt acgcgtcatt gctggcagct taggaatagt attatcgatc cctatctcgg
     8161 tcctcttttt tctgactgta aggaggctga aacgatgaat gtacttatta tacttgttgg
     8221 gattttagca atcagcgtgc tacttattgg cggtacgcaa ggtatgtata ttcttttagg
     8281 cttattcatc aatttaggga tctttttcct gttgttattc ggttatcatc agaagtggcc
     8341 gatactggca ttgtcggtca taggattttt attgattgct gtggttatct tattttttat
     8401 caacggttat aatctaaaaa tgcgtgcagc ttttgtgagt atactgatct ttttattttg
     8461 tttcctacta ttgcttccta tcactgattt tttagcaatc caaggtttta catcggtaga
     8521 actagaagaa ttatctggct tagataagac tttagcgatt gattttagat tacttacgag
     8581 atcgttgctt ctgattagtc ttagtggtgc tgttttagat gcgagtgtag caatttctag
     8641 tgggacattc gaagtatatc aggcaaatcc gcaattatct ttttaccaat tgcgccattc
     8701 tagtttcgct attgctaaaa aaatattggc gtcaacagta atgacacttc tttttgcatt
     8761 catgggaagt tctttagcgt tgatcctctg gtttattgat ttagacattc cttttcagca
     8821 agtgatcaat gaaaaatcat ttgttctaga atatacgatg gctattctta ccacattgtc
     8881 cgcactactt gttcttcctt taacctgcgt gactacagca tatctcttta caaaaaagaa
     8941 agaacaactc aagatggagt aaaagttaca acttaagtcc tatgacattt ttactgaatc
     9001 agtgcataat atggatgtaa ggaaaagctt gcaaacaatt ttgcttaagg aggcaatttc
     9061 catgaaagaa agagaacgta ttttagattt agtgaaaaaa ggaatccttt cgactgaaga
     9121 aggcttagat ttattagaaa gcatggcaac tgaaaaagac gaaaagcaaa tcaaaaaaga
     9181 agcagacaag gtaactgcta gtcataaaga aaaagatcag gcaagtcaat tgatcgataa
     9241 acttgaaaac ggcgaagaag aaatttctga gccagttgat ccaaaggaaa gagaaagaca
     9301 agatcaagaa aacttagaaa agatcttaga tgaactagca actgaagcga ataaaacttc
     9361 ggcgcgtcta gatgaagtaa atgctcaatt cgctgacaat aaggcagcac gcaatgagaa
     9421 acaggaagca ttgatgcagc tgaatacaaa agaagaatta ggcgagttga cagaagaaga
     9481 attagcacag cgtcagacgt tagaagcaga aatcaaagaa ttagaagcta gtggcgatac
     9541 actgttagaa gaacaattga aattagaagc agaattaaaa gatatccgta agaatcaatg
     9601 gtctgagaaa aaagaaacgt ttactgataa gtttgaattg ccagatgatt ggaaagatca
     9661 agcaacagat acactgaatc aagtaggcga aaaaatgtca gaagctggat cacagttagg
     9721 caaattcttg aagaaaacat tccaaactgt ttcagaaaca gtgaatgaca acatggaatg
     9781 gaaagatgtc agcttacgtg tccctgggat tgcgacaacg aaatttgagc atgaatttta
     9841 ctatgaagct cctgcagcaa gtatccttga tatcaaggca gcaaacggaa acgtgacgtt
     9901 aaaaacgtgg gacagcaacg atgtgaaagt tgaagctaag atcaaacttt acggaaaaat
     9961 gggtgctgaa ccatttgaag cattctctga acggagccag attgaggtta atgaagatca
    10021 tatctccttc caaattccaa ataaacgtgt gcgtgcagac cttgttttct acttacctaa
    10081 acgagtatac gaccacgcag ctattaaact tctaaatggc aacatcatga ttgaaacatt
    10141 ggaagctaaa gatatttata caaaatctac gaatggtaac atcatcgtgg accaattaac
    10201 tgcaacgatg ctggaagtag aaggtgtaaa cggcaatatc gatattcgaa acgggaacat
    10261 cttagattct attatcgaaa cagtaaatgg tacagtgaca tttggtgcga cagcagaaaa
    10321 cctttctgtg tctttggtaa atggtgatgt acgtcttacg attaaagaag ataatttgaa
    10381 aaaagtagaa gcaagttcag tgaacggaaa tgtcaaagtc gctttaccag atggcatcgg
    10441 tttggaagga catgctaaaa caagtcttgg aagtatcaac agccgtctgt ccaattacga
    10501 agtagttcgt gagaaaaaag aacggacaaa ccaaatgttg caattccgtc gtgtttcaga
    10561 tggtgaaatc gcgcaagtgc aactttcaac gacaacagga agtatctatt taaaagatac
    10621 agataaataa agaggttgtg aaagagatgt tcagccttga gcaataagaa gcgattttgt
    10681 aaaatagctt cccctatttt acaaaattgt gccttattgc cgatagtcta tctcttgaac
    10741 accgtttacc aagagattat gaacctgcca attatcgccg agtcacaagg aataatttca
    10801 gaaaaagctc gtcacgtttt atgaatatgg taatttgtga ccgagaattt tgcagaaaga
    10861 caccgattaa gaagaaggtt gtcacaacga ttttcttcat ttaaaatagt agaacagagg
    10921 tgagacatga tgaaaaaaaa gttgacaaaa tctaataaaa atgttgtttt gacagggact
    10981 ttagcaggaa taggagaata catcgggatt gatccgactg ttatccgtgt gctttatgta
    11041 ttcctgagct tagttgcttt tggaagtcca attattttat acattttatt agctttgatt
    11101 atcccatctg cgcgttctgc acgaacaaac tatggacata acaatgcata ttatgaaaaa
    11161 aacaattatc gtgaacaaac tcgtgaacaa acaaaaaaac gcaaagaagc ggaaaaaatc
    11221 gatgatgacg aatggagtga cttttaatgt cgtactttca acgtatttta gttaatacgt
    11281 taacattcat atcattggct gttctttttc ctgcgatggt ccatgtgagc agtatttgga
    11341 cggccgttct tgccagcttc gtattgtctg tgttgaatat gtttgtcaaa ccgattttga
    11401 cgatactgtc cctgcccttt acattagtca cattgggatt gtttacgttt gttatcaatg
    11461 cagcgattct taaaatgacc tccttttttg tgggagcaga acattttagt ttttcttcat
    11521 tcggtgcagc gttgttagta gctatcttga tgtcgcttgt caatgtaatt gtcagcgaac
    11581 ataacagtag aaaataaaag aaagctgtgc cgaactatcg gcgcagtttt tttatttaga
    11641 atcaggaatg cgttttctat tacattggta attaaataaa aacgtttttt ttaattggta
    11701 gtacttttgg ctagacaatg ataaaatgat agagaacact ctaatagaaa tgaggtagca
    11761 tgaatgtcag aagttgtaaa agttagccaa ttagtgaaaa aattatcttt ggaaatcgtg
    11821 acaggcgacg agaaaagctt agaccgtgtc atcgtaactg gggacatctc acgcccagga
    11881 ctagagttga ctggttattt taattattat tcccataatc gtttacagct atttggcagt
    11941 aaagaaatca gttttgcgga acggatgatc ccagaagagc gtttgatcat catgagaaga
    12001 ttatgtagtg aagatatgcc cgcatttatt atttcaagag gattaccagc tccagaagaa
    12061 atgatccagg cagcaaatga aaatgggata gctgtactgc gttctcccat ctcaacttct
    12121 cgtttattag gagaattatc cagttattta gatggtcgcc ttgcccctcg aacaagtgtc
    12181 cacggtgttt tagtggatgt ttacggatta ggtgtactga ttcaaggtga cagtgggata
    12241 ggaaaaagtg aaacagcgtt ggagttgatc aaacgcggcc atcgattgat cgcagacgat
    12301 cgagtagatg tttatcagca agatgaatta acggtgatcg gagaaccgcc taaaatcctg
    12361 gaacatctga ttgaaatccg aggaatcggg atcattgatg taatgaatct ttttggtgcg
    12421 agtgcggtac gtggctctat gcaagtccag ttggctgttt atctggaagc atgggcaaaa
    12481 gataagaaat acgaccgtct gggctctgaa gacacgaccg ttgaaatcgc ggaagtgggc
    12541 attccacaaa taaaaatccc tgtcaaaaca ggacgaaacg ttgcaatcat cattgaagta
    12601 gccgcgatga atttccgcgc acgtacaatg ggctttgacg caacgaaaac atttgaagaa
    12661 agattaagtc gcttgattga agaaaactca ggcaacgact agcaaggagt gacaatatgt
    12721 tagcacaaat caatcgagtt gcttttgagc ttgtagggat tcctatctac tggtatgcat
    12781 tgatcatcgt atcaggtatc attatcgcta tgtggcttag cagcagagag gctgttcggg
    12841 taggattaaa agcagatgat gtgacagatt ttatgttgtg gggattgcct atctccatta
    12901 ttggcgctcg cttatattat gttttatttg atcttcccca gtatatagct gatccaatcc
    12961 aaatattcaa tatccgttca ggaggcttag ctatttatgg cggactgatt gcaggcgcga
    13021 tcacgctgta tttttttact aaatatcatt ttatttcttt gtggactttt ttagatatcg
    13081 ctgcaccaag tgtattatta gcacaggcaa tcggtcgttg gggaaatttc atgaaccacg
    13141 aagcatatgg accagacacg acgcgaagct tcttagaaag tctgcattta ccgcaattca
    13201 tcattgataa tatgtatatc gaagggatct atcgtcaacc aacatttctt tatgaatcac
    13261 tttggagttt gattggattt attttgcttt tgtttttacg aaagaaacca caccttttga
    13321 aaaaaggcga agtcctttta tgttatctaa tgtggtatag tttcgggcgt ttctttattg
    13381 aaggattacg tacagacagt ctttactttt taggtgtgat ccgtatttct caattattat
    13441 ctgctgtgtt atttgtcggt acattgattc tatttatttg gcgcagaaaa aaaggaaatt
    13501 taccggatta tgaccgctca ttaggaaaaa atcaacaaat tatctgattt ttagagcgtt
    13561 aaaaatgaaa aaaatttcag gagtggtaaa atgaattata gagtagcggt tttaggacca
    13621 ggctcttggg gtactgcttt ggcacaagtc ctcgccgaaa acggacatga cgttcggatt
    13681 tggggacatc gtgcttcaca agtagaagaa atcaacaccc aacacacgaa tcaacgttat
    13741 ttaccagatc attatctacc cgattccatc caagcattca ccaatatgga agatgcagta
    13801 aaagacgcag atgcggtgct attcgtcatt ccaacgaaag caatacgttc tgttgcaaaa
    13861 gaactagtgc ctaaattgaa tacaaagcca gtgatcatcc atgcaagtaa aggattagaa
    13921 cagggaacac ataaaaggat ctctgacatc ttgttagagg aaatcccagc agaaaaaaga
    13981 aaagctgttg ttgttctttc tggaccaagc catgcagaag aagtagcaaa acatgacatc
    14041 actacgatca cagctgcaag tacagatgca aaagcagcaa aatatgtaca agagctgttc
    14101 atgaataatt attttcggat ctataccaat cctgatgtga tcggtgtaga gatgggggca
    14161 gctttgaaaa atattattgc tattggtgct ggcgctattc acggcttagg gtttggcgat
    14221 aatgcaaagg cagcaatcat gacgcgcgga ttagctgaga tcagccgttt aggtgtagca
    14281 atgggtgcca acccgctgac ctttatcgga ttaagcggaa taggcgatct agtcgttact
    14341 tgcacaagtg ttcattctag gaactggcgc gcaggcaact tgttaggaca agggcagccc
    14401 ctagaagagg tgctagccaa tatgggaatg gtcgttgaag gtgtgaatac tacccaagca
    14461 gcgatggaac tagcccaaat gatggaaatc gaaatgccca tcactcaaac aatctatgaa
    14521 gtgctgtatg aacaaaaaga aatcaaaaca gccgcaaaag aaatcatgtt acgtgatgga
    14581 aaaaaagaaa atgaattctc attaacaatt ttttgaagga gcgaatgtat atgaaagtta
    14641 aaaaagcagt tattccagca gcaggattag gaacaagatt tttaccagca acaaaagcta
    14701 tggcaaagga aatgttgcca attgttgata aaccaacaat ccagttcatc gttgaagaag
    14761 cgttgaaatc aggtatcgaa gacatcttga tcgttacagg aaaagctaaa cgaccaatcg
    14821 aagatcactt tgattcaaat attgaattag aaacaaacct aagtgaaaaa ggaaagaccg
    14881 aattattgaa attagtagaa gaaacgacag atgtcaactt gcatttcatc cgtcaatctc
    14941 atccaaaggg tttaggacat gccgttcttc aagcaagagc ctttattgga aatgaaccat
    15001 ttgtcgttat gctaggcgat gatataatgg aagacgaagt accattgaca cagcaattaa
    15061 tcgatgatta cgaacgtact catgcttcta cgatcgccgt tatgcaagtt cctcatgatg
    15121 aaacttcaaa atacggaatt atcaatccag gagaagtatt ggaagatggg ttatacaatg
    15181 tcaaaaactt tgttgaaaaa ccagatccaa gcaaagcacc aagtgattta gctattattg
    15241 gacgttactt attgactcca gaaatttttg atgtattgtc cgagcaacaa ccaggagccg
    15301 gaaatgaaat ccaactaact gacgcaatcg atacattgaa taaaacacaa cgcgtatttg
    15361 cccgtaaatt cacaggtaaa cgttacgatg tcggtgacaa atttggtttt atgaaaacaa
    15421 gtattgaata tggtctaaca catccagaag taggtgcacc tttacgtgag tacatcctgt
    15481 ctttaggtaa aacattagaa gcagaaggaa gcaagatcga tcataatcca aaaggaataa
    15541 cagaaatcaa agaagaaaag aaaaaataac ccttttttct aacgagcttc agctgctaat
    15601 tgaataaaaa gtagttgaag ctcgttttta tttgtttaaa aaacacgaaa atgctatcct
    15661 tatagaaaag agagacaaga agggagtttg atcatgtcag gaggagaaat tgcaggattg
    15721 atcgcagcaa tcgcctttgc gatacttgtc gtttttatcg ttcgtgtgtt gttgcaagtt
    15781 tctaaaactt tagaaaaagt agatcagact gtagcagaag caaatacgac gatcgaaatc
    15841 gtcacaaaag acgtagatct tttgtctaga caagtagaag gcttactggt caaaagtaat
    15901 gaactgctct tggatatcaa tggaaaagtc gcaacgatcg atccattgtt cacagcagtt
    15961 gctgatttaa gtgaaagtgt atcagaactt aatcattcta gtaaaaacat cgcgacgaaa
    16021 gtcggcggaa tcggtaaaac gacagctaaa gcgacggttg ccggaaaagt cggtgggaca
    16081 gcgatgaaat tttttaaaaa caaaaaacat actgaaacga caggaggaaa ataaaaatgg
    16141 ctaaaaaagg tggattttta ttaggtgcag tagtcggagg cacagccgca gcaatcgccg
    16201 cgttattgtt agcaccaaaa tctggtaaag aacttcggga tgaattagct gcccaagcgg
    16261 atgatttgat ggatatggct tcagattaca cagatatggc gaaagaaaaa tcgactgagt
    16321 taacaggtat tgcaaaagaa aaagcgcaag atctttctca acaagcaggt gatctagcag
    16381 gtagtgtcaa acaaaaagta agtgaatcag cagatgaagc aggaagtgaa tctgacgata
    16441 tcttgttaaa tcttaaagaa caatcagctg acttatctaa tcgtttcaaa aaaacggcag
    16501 aagatatgaa aaaacctgca gatgaattag gaagtattgc taaagatact tcagaagata
    16561 tcatgattga tgtaaaagac gctgctggta aagcaggaga tactgtcaat gaaggaatgt
    16621 cagaagcaaa accagttgca gatgactcaa aagaaatgat gaaaatggca aaagaagatg
    16681 cgaaagaagc taaaaaagaa ataaaagata caatgacaga ttcatctaca aatcactaaa
    16741 atcacaaatt tatagaataa aaaggtggga gcggactagc tctcaccttt tttactctta
    16801 tttttctaaa acaagtaatt cttttggtgt agtcgtgaat ggcacacagc catcgcttgt
    16861 cacatataca cagtcttcga tacgtacccc tactttttca ggaatataga ttcctggttc
    16921 aagagagaag cacatccctt cttcgattac taagtcattt ccttctacaa gggaagggaa
    16981 ttcgtggacg gttgttccga tcccgtgacc taaacggtga ttgaaatatt cgccgtagcc
    17041 atagctgttg atcacgtttc gtgcgatttg atcaagttca cctgctgtca cacctggacg
    17101 gacggcttct tgtgcggcaa gctgtgcttc aagagtgatg ttgtagattt tttcttggaa
    17161 gtcggttggt ttttgataag caattgtacg agtagcatca ctgcaatagc cattccaaac
    17221 aacgccaaga tcgaataaca caagttcatt cggtgaaatc ttcgtatttc ctggtgtacc
    17281 atgagggctt gcagcatttg ctcctgcaag gactaatgtg tcgaaagaca tttgtgaaac
    17341 accttgtttt ttcaattggt attcaatttc tgcaatgatc gcttgttcag tcacgccttc
    17401 tttcactgct ttaaagccaa tctcaaatgc aacatcggcc catttaccag cttcgatcag
    17461 tttgtctttt tcctctagag ttttgatcag ttgtagacgt tggatgatgg aagttagatc
    17521 agtgtaagaa tccgttcccg tcaagatatc agagatagca tcgaagcgct cgacagtcaa
    17581 ggcgttcttt tcaatcccta cattgccagg ctgaccatag cgagcacgaa gttcttttcc
    17641 gatgatctcc caagggtttt cgctgtctaa atagccacgg acatcatacg cccaactact
    17701 gttttgagca tcttcaactt ccaaagcagg agtgaaaagg aaaggatctt gttcaagagg
    17761 gataaaaaga gctagtacac gctcatgagg atcactttcg tatccggaaa agtaagcgat
    17821 gtggccaggg tcgctgatat aagccaattc gacttggtgt tgtgccatcc attgacgtag
    17881 ttcattgatt tttacgttat tcatttggca acctactttc taaattaatt ttgtattcaa
    17941 taattgtacc actgatttat taaaagacca acgttctaaa aactgaaaaa agtaaaaatt
    18001 ttcatgaaaa cagagataaa acggttgcaa ttcttgagat tatttgatat tctattttag
    18061 aaatgtaaga cgtattttca gaaaacatat aaaatatgaa taggagaaca caaatggaaa
    18121 agcaaacaat cactatctat gatgtagcga gagaggcaaa tgtttcgatg gctactgttt
    18181 cacgtgtcgt aaatggcaat ccaaacgtta aaccagcaac aagaaaaaaa gtcttggaag
    18241 ttatcgatcg cttggattat cgtccaaacg cagttgcgcg tggcttggca agtaagaaaa
    18301 caacaacagt tggtgtaatc atccctgatg tcagcaatat gttctttgct tctttagcac
    18361 gtggtatcga tgatgtggca acaatgtaca aatacaatat tatcttagca aattcagatg
    18421 gcaacgacca aaaagaagtc aatgtattga ataacttact ggctaagcaa gtggacggcg
    18481 tgatcttcat gggtcaccat atcacagacg aaatccgtgg agaattttca cgttcaaaaa
    18541 ctcctgttgt attagctggc tctattgatc cagatgaaca agtaggtagc gtaaacattg
    18601 attatgtatc cgcaacaaaa gatgcagtaa acaaattggc aaaaagcggc aataaaaaaa
    18661 tcgcttttgt cagtggtgcg ttgatcgatc ccatcaatgg acaaaatcgt ctgaaaggct
    18721 ataaagaagc attaagcgaa aataacctac catatagcga aggattgatc tttgaagcac
    18781 aatacaactt caaagatggt ctatctctag tcgatcggat ccataatagc ggtgcaacag
    18841 ctgtctatgt ttcagatgat gaattagcca tcggtatttt ggatggttta ttagaccgtg
    18901 gcgtgaaagt accggaagaa ttcgaaatca tcacaagtaa caattcacta ctgacagaag
    18961 tagctcgtcc tcgtttaaca agtatcacac aaccattata cgatttaggg gcggtatcta
    19021 tgcgcctatt gacgaaaatg atgaataaag aagaagtcga agaaaaaacg attatcttgc
    19081 cttatggaat cgaagaaaaa ggatctacaa aataataaaa ggacaaccat tcagaggttg
    19141 tgtgcctgcc gtttaactct gcgttacaag gagcaatttc aaaaaacagc atctcatgtt
    19201 tgatgaatat tgtgactcgt gattcggagt tggcaggaga acaccgttta tttgggaaaa
    19261 ggggttatga caaaattttg ttataatcct ttttttgtgt cttaaaaaag agagaaagtg
    19321 gttgaaatta catttatcgt atataaaaca atccgtgtat agatgctgca ttttgttcac
    19381 caccatcaaa aaaataaggg actgtgacaa tactattgtc acaatccctt attactgtgt
    19441 attctattaa atcaatcatc tttctcatct gattctttag ttgcttgatt cgctcttgca
    19501 taagctgaag cggtgttcca gtaatctgta tagttttcat cggtaccgcc aacgccaaat
    19561 ttaaaggcgc ttttcttcgg tccatttttc gcccatagcg attcaactgt ttttcctggt
    19621 gtttggatgg attttttatt gacagtaact tttccaggtg tttgaccagt aaatccagat
    19681 acttttttct tcatcacatc agatgataat tcgaatttct catcagtgcc aaaaatgtca
    19741 ggttttgctt ggtaagcttg attgatcaga ttggcaagat aattagccga acgtatcctt
    19801 gctttgctgt ccattccagt attgtcatcg tgccctgccc agctactgat ggtaatagac
    19861 ggagtagaga caattagcca tgaatctcgg tattcattgg tagaaccagt ttttccgacc
    19921 caatctgctt ttcctagatt tccgtttaag cttgagatgg catctttgaa tggtgtagtg
    19981 atttttgcat tgatcacact acgcatcatg tcattcatga tcgatgccgt tgcttccgaa
    20041 tagatccgga cagagttgct ttcgtgttta taaattacat ttcctgcgtt atctgtgatg
    20101 gaatcgatga tatagccttc ttcatagacg ccgccgtttg ctaaagcctg aaatccgttg
    20161 gtctgttgaa gcgtcgtgac atctactgta cctaaaggag cagattcaac gccccaattg
    20221 tcatttgccg ggtagttcat ctttgccaag tagtgctcgt aagcatattg tttcgatcca
    20281 ccggaattca acacatcttg atataagtga taagccggga tattgttgga ccattctagt
    20341 gactcacgga cggtttggaa ggtgttcgat ccttcgttag tagcattgac gattttctcg
    20401 ccagcatttt ctccttcttg ccaagttgtt gcataatctg atacacggga ttctgatccg
    20461 ataagcccca tgtcgattgc aggtccataa acaagaactg gtttgattga tgagccagct
    20521 tgacgttctg tatcgaaggc atggttattt tgattctgac tgtaattccg tccgccaacg
    20581 aatccgtaga tgcggccagt tctgttatcc atcaatacat ttccggtttc tacttgactg
    20641 ccagcaccat catctaacat gtatccataa ttttcaacgc cattttgcaa tgcagcatag
    20701 acatctttat cgattgtcga atggattgta tatcctttat tttggatcgt ttgctgtgcc
    20761 ttttgataat aagcgtcact gaccgatgaa tcagacatat cagcgttgtc tttttcagcc
    20821 aattgttgtg caatgattct tgttgcttca ttcatcacag tataatacaa gaattctctg
    20881 tcagtctgtt ctgcgatttg ttgtggtaga aagtcttttg taaggtcata ggcttttgct
    20941 tcttcgtatt gttctttcgt aatttggcct tcacgataca tactgaataa aacaaaatct
    21001 tttctggcaa ggccggcaga aagatcttct tttagcgcgc ctgtgttcgt gtatggacta
    21061 tacgtgatcg ggctttgggg taatccagcg atataagcag cttgcggcag cgtcacatct
    21121 tttgcatcta caccaaagat tccttttgca gcttcctgga ctccagcaat gttttgtccc
    21181 ttattattac gaccaaatgg tgagacattc aaatacgtag caatgatctc atcttttgaa
    21241 aagtattttt ctacttgtgc agaaagaatg atctcgtttg ctttacgttt gaatgttgtt
    21301 tcatctgtaa ggatctgctg tttgactaat tgctgtgtca aagttgatcc accagaagag
    21361 ccaataccag tagcttcaga tactaatgct cgtaaaacag ctttaggaac gtaaccttgg
    21421 tgtttatcga agtattcatc ctctgtgctg ataattgcag ttttcaataa aggtgaaatc
    21481 ttatcagatg aaatagtcgt gcgcatcaga tctgtctgga tttttgatat ctctgtatta
    21541 tctgcataaa caatctttga cgtttctgta atgtttccta gttctgtctg cagtgctttt
    21601 ttagtcggca attgtgtgtc ttcgacgaga taggcaaaat agccagcgcc tattccaata
    21661 cctagagctg cgaacatcaa aagaagaaca gtaataaaaa cagtcaatga ttgaaaaact
    21721 ctaaaaataa tcttaggaac aaaccatttt tctttttttc ttctattctg tttttttcga
    21781 gtatttttgc gtggcaaaag ggtcacctca actaatggtt ttatttcgct aattatagca
    21841 aataatagaa caaaaaaaca gacaaggctg tttttggcag gaatttcttt tttaaaagag
    21901 ttaaaacgtg agaaaatgat ctttattata aaagagttct taagcttttc tttagagaaa
    21961 atttcagtta ctatggagaa cagggcaaag aaaaatcaat cattttcttt gtatatcatc
    22021 ttaccaatat ataggaattg ttagtaacaa ttgcctttca aggaggaatc catccatgtt
    22081 attaggctca cacgtcagca tgagcggaaa gaaaatgctg ttaggagcag cagaagaagc
    22141 agccagttat ggatcttcga cttttatgat ctatacaggc gcccctcaaa acactagaag
    22201 aaaagccatc gaagagatga atattgatgc cggaaaagcg tacatggcag aaaaaggtct
    22261 aacaaatatc gtcgttcatg ccccttatat cattaatttg gggaatacga tcaaaccaga
    22321 aatgtttcct tttgccgtcc aatttctgag agaagagatt gttcgtgcgg aagctttagg
    22381 cgctactcaa attacacttc acccaggtgc tcatgtaggg gcaggaacag aagccggaat
    22441 tgctagaatt attgaaggct tgaatgaagt tttgacaaaa gaacagacgg cacaaatcgc
    22501 tttggagaca atggcaggta aaggtacgga aatcggtcga acatttgagg aattagcgca
    22561 aatcatcgat ggagtaaaat tgaatgaaaa attatccgtt acttttgaca cctgtcatac
    22621 gaatgatgcc ggttataata ttaaagaaga cttttcaggt gtaatggaag agtttgataa
    22681 gatcatcggg ttagatcggc ttaaagtact ccatatcaat gattctaaaa atccgcaagg
    22741 ttcgcataaa gaccggcacg ccaatattgg ttttggaaca atcggattcg acacgctgaa
    22801 taagatcgtc catgatccaa tgtttgacaa tatttcgaaa atcttggaaa ctccctatgt
    22861 aggcaaagat aaaaagaaag cttacgcacc atatggaaaa gaaatcgcca tgttcaaagc
    22921 gcaaacattc gatccagaag tgtttcctga gttattagca gaagaagtga tactctaaga
    22981 ttttctatct gacagcagca aaaaaggacc aggcacattg ccttgtcctt ttttgtttaa
    23041 gaaacaaagg agtacttttt gcagtctttt tcatcatgca tcttctcccc cctcttttta
    23101 aatttagaca aacaaggtat actggtagaa aagtaaaaaa acgaaaagtt tcacaagcaa
    23161 gtggcaaaac gtgattggaa ggacgaaaag atgctgaaca ctttaaaacc cttgatcaac
    23221 caactaaaaa agaaacatac atactttatt cttggcatag ccctattatt tacaattatt
    23281 cgtttgtatt tatatagtaa agcaatctat ggaacagaca tgtcggcaac ctatgatgac
    23341 cagctgctga ttcgctatgc tcaaaatatt ttggacggta aatggctagg ggagtatacg
    23401 actacaacac tttccaaagg aatctcctac agcttatttt tagtacttgc acataaactg
    23461 catctttctt atgcagtttt gttcggcatg ttcagtattt ttgccagtat attggttgca
    23521 ctcgcgttta aaccgattgt gaagaacaag tatcttcttt taagtatcta tttttatttt
    23581 ttgttttctc cgattagttt tacacatgaa tattccactc gaacatatcg taatgccctc
    23641 gtgattccgg ctgttgagat cattatcgct tgtttattag ctatttatta ccgcaaatac
    23701 ggttctagca gaaaattgct gccatggttt ttagggcttt ctgtcgcttt tcctttcttt
    23761 tggttcattc gcgaagattc tatctggctt cttcctttta ctgtagtagc tttgggtatc
    23821 agtttcgtcc agatcttgct agggcaagac ttttctttca aagaagtaaa gaagttaaaa
    23881 aaacgactct ttcttctctc gaaacgggaa tggctgaaac tttcattgtt tttacttcct
    23941 atagttggct tttccatcgt ctatcaatgg atcgaatata aaaatgagac aacatacggt
    24001 attgcaacca tcaatgaccg ttccagtgga gaatttggac gtttgatgaa acaactgatc
    24061 cgaatagatg atgaaacgaa cttgaataaa gaaaacagtg agatatgggt ctcacatgct
    24121 gcactaaata aagcattagc cgcatctcct acattccata ctgtttctac ccgtattgac
    24181 gaattatata cgactcacgc gtggactcaa ggtggaaaag taaaagaatt acccggagat
    24241 atcatctttt gggcattgcg agatgtggta agcgaatctg gttattacca aaacaatgca
    24301 aaagaaacca atgatttttg gaaaaaagtc aaccaagaat tagaagaagc ctatcacaag
    24361 ggaacaatca aaaagaaaaa agagttctat ctgatgggat caggagatgg aaagacgatg
    24421 agtgatctgc caatcgtatt cgatttcctt caatcaggat acgcttatgc atggcgatat
    24481 aaagatttcc atcaaggagg aaattatagt ttcggaacgc aagaacaagt caaacaggct
    24541 caagaattat taaaccttcc gctaatggag aattggatcg atagtaatga accgagaaca
    24601 cctaatttcc aattaacaaa atcagcaaaa gtcgcgaatg tcatcataaa aatttaccaa
    24661 gttctggcta ttcccgtgct gattgcttca atagcaggga tattacttat gatactagga
    24721 accgtatttg caaaagctga tcgtgcctat tatagttcct ctttgattgt ggtcaccgga
    24781 ttaatactga cacaattggt ttttcttttc ggtgtctcat ggttttgttc ttatgcgcca
    24841 gaaaaaaagg actatttttt aagtgtctac actggtgcag gcgtgccatt aacgcaattc
    24901 atcgtagtaa caagcatgct agttttcagt aaattaaatc tgaaaaaagc tttatcttct
    24961 aaataaaaag acccttggta gctagatttt taacctaact atcaagggtc tttgtgtgga
    25021 aaaatcagtt tttattcgat ttcttctacc gtttcctgca aaagctgttc atattttttg
    25081 tttcctttat ataaatcacc aatcgtccat gccaacagga gaaggacaag aacaatgatt
    25141 acataagcga tgtgatcagc caatgctagt tgagcggaag tggcttgctc gccgaagaaa
    25201 ctttcgatct gtcctggtaa tcctcgtaag ttcaagtaag ttagactaat cacagagaac
    25261 cagccaagaa gttttacgat gaatgaattt ttaaatcggc tacccatctc cgcttggctg
    25321 ttggtcatca tcagtaaagg aatcatcgaa aacggcaacg caaaagccaa gaacacctgg
    25381 gattcattca ttaagttatt caaagcggca tgttcttcga tcgttccttt tgtacttgta
    25441 aacaaaacac aaatcaatac aggaatcaca gaaagcaatc gtgtgaccag tcttcttagc
    25501 caaatcggga tacgcatatg gatgaatcct tccatgatga cttgaccggt caacgtacca
    25561 gtaatagtag agttctggcc agaagcaaga agtgctacag caaataaagt ggacaagata
    25621 ccagattttg ctacagcaat caaaatacca ttgctcatag ctgatggatc agataaagct
    25681 tcatacagac caaaaaagga aggatcttgg acagaaccgg ttttgaagac agcgacaccc
    25741 atgatcaata gcagtgcatt gactagaaaa gccattgaca actggatatt agaatcccaa
    25801 gtagaaaaac gtacagcttg tgctacatct tcttcgtttt ggtgatcgat cttccgagtt
    25861 tgtgaaactg ctgaatgaag atataagtta tgcggcatga ctgttgcacc aataatccct
    25921 aaagctccag tcagcggagt ttcgccgccg atgaccggtg agtctgagaa tgtatgactg
    25981 ttagggataa agccagcaaa taaactgtgc caatctgggt tagacaagcc aacttgatag
    26041 acaaaaacga ataaaatgac gaaaattaag cagacaacca gtgcttcgat cttacgaaac
    26101 ccgacttttg tcagtagtaa gagaacgaaa acatctaata cagtaataaa gacggcaatc
    26161 accaatggaa tatcaaaaag taagtagagg gcgattgctg caccaatgac ttctgcaata
    26221 tctgttgcca taatggctaa ttcagtcaag atccataaaa tgacacctaa tgtcttactt
    26281 gtgcgtgctc ggattgcctg tgccaaatcc atttggctca cgattcctaa ttttgctgcc
    26341 atatactgta aaagcatagc aatcagacta gacatcaaaa taactgacat caataaatat
    26401 tggaaatttt gtcctccggt gatcgaagta gaccagttac ccgggtccat atacccaact
    26461 gctactaacg caccaggtcc agagtaagca agaagtgttt taaaaaaact tttccccttt
    26521 ggtacatcaa ctgttccatt tacttcagct aaagataaac catttgtatg ttcaataagt
    26581 cgatgcttat gaaattgttt attggattga ttattcaaga aaaaaccacc tttcctattt
    26641 atctacgatt acattctaca cctcaaaaag aaaaaattaa agaaaaagat tcggtacgcc
    26701 taattttttt tcatggcgcg tcttgctaga tgacgaaaga aattttgttc tcctctaaaa
    26761 aaaccactat tttttgcatg aaactaaaga atacgccttt cttttttcaa taaactttag
    26821 taaaataagt cattgagagc aattcggaaa tagaaaggag agatttatat gttatcaaca
    26881 ggtattgtcg tacttattgc tattattgct ttgctaatcg gcgcagtagg tggctttttc
    26941 ctagcgcgta aatatatgaa agactattta gaaaaaaatc ctcccgttaa tgaggacatg
    27001 ttgcgatcaa tgatgatgtc aatggggcaa aaaccatctg agaagaaaat tcgccagatg
    27061 atgcagcaaa tgaagaacca agggaaaaaa taggttctga cccaacgact ttttacgggc
    27121 tgattttttc agtccgtttt ttatttcgta atttgttaga ctattcagat tattcagcaa
    27181 aaaagacaag cacagacaat cctaggctct tgataaagac aaaggaggag taaaatgtcg
    27241 atttttagaa aattaggctg gttttttcgt caggaaaaga aacattattt aattggggtt
    27301 ttctcgctat tcgcagtggc cttggtacag ttagttcctc caaaagtgat tgggatcatc
    27361 attgatgaga tcgccgataa aaatttatca ataaagatca ttttcgggtg gatcttccta
    27421 ttgattatcg ctgctttttt acaatatctt tttcgttata tctggcggac gaatatttgg
    27481 ggaagtgcag ctcgattgga gaaagaactt cggcagcagt tattcaaaca ttttacagaa
    27541 atggacaatc tcttttacca aaaatatcgg actggggatt tgatggctca cgcaacgaat
    27601 gatttgaatg cgatccagaa tgtagcagga gctggtatct tgacctttgc agattcagtg
    27661 attacgggcg gaacaacgat cattgcgatg attttgttta ttgattggcg cttaacactg
    27721 attgcgctga ttcctctgcc tttgctggcc gtcacttcac gagtgctggg gtcaaaactc
    27781 catgatgctt ttcgtgattc gcaagaagct ttttctagta tcaatgataa gacacaagaa
    27841 agtatcactg ggatcaaagt cattaaaaca tttggacaag aaaaagaaga tctagcggat
    27901 tttgaagaaa agattgaaga tgcaattatt aaaaacaaac gagtcaattt tttagactcc
    27961 ttatttgatc cattcattac cttgattatc ggtctgtctt atgttgcaac aattatttta
    28021 ggtggaaagt atgtcttgga aggcacgatc tcgatcgggc agatggtctc atttattagt
    28081 tatatcggta tgcttgtttg gccgatgttt gcgattggtc gattgttcaa cgtattggaa
    28141 agaggaaatg cttcttatga tcgcgtcaat gaacttttgc atgaaaagac gcatatcgtg
    28201 gaacaactgg gtgcagtgca gaccccggca aaaggcgagc tgaatgtagc tatttcctct
    28261 tttaattatc cgaaagaaga atcaccagca ttagaacaaa tcaagtttgc gatccaagaa
    28321 ggagaaacat taggtattgt tgggaaaact ggtgcgggaa aaacgacgat catcaaatta
    28381 ctgatgcgtg aatacgatga ttatcaaggg aaaatccaat ttggcggacg ttcgatcaaa
    28441 gaatattcat tgaatgcttt attaggatca atcggctatg tgcctcagga tcacttcttg
    28501 ttttcgatga ccattcgaga taatatccgt ttttctaatc caaaactttc agaagaagaa
    28561 gtagagcatg cggcttcttt agcatttatc aacaatgaaa tcaaggaatt accaaaaggt
    28621 tatgatacct tagttggaga acgtggagtc tctttatctg gcggacagaa acaaaggatt
    28681 tcgattgctc gagcattgat caaaaatcct gaattattaa tattagatga tgcactctct
    28741 gcagtagatg ccaaaacaga agaagctatc ttgcataatt taaaagaaat aagaaaagag
    28801 aaaacaacta tcattgcagc tcaccgttta agtagcgtga tgcatgcaaa agaaatcatt
    28861 gttgtcgatc aagggaaaat cattgaacga ggaacccatc atgagctgct tgcactaggc
    28921 ggctggtatg aacaaatgtg ggagaaacag caattagaag cgaagataga aggaggcgag
    28981 gcctgatgga tgaaaattat caatcggaat ggacaaaatc gattccactt aaagaacaga
    29041 aaaagatcat caaacgactg atgaagttcg ccaaaccttt tagaagaacg ttcatcgttg
    29101 ctatcttatt tgcgtttgct ttgtctgtga tcaatgtcct attgccaagg atcatccaaa
    29161 cttttatgga tgatcatctg gctaaacagt cggctacgac acaagttatt ttgttttttg
    29221 ctggtatgta cttatttgga gtcatagtaa aaagtatcat ttggtttttc caatggtatt
    29281 tgtattccat ggcctcattg aagacctatc agtatattag ggtaaaatta tttgaaaagc
    29341 tgcatacact aggtatgcgt tattttgatc agacaccggc aggatcaacc gtttctcgag
    29401 tgacgaatga cacagaaacg ctatttgaat tttggtacgt ctttttgatg gtattgactg
    29461 ggatctttgc agtcgtttct tcattccttg ccatgtttca aatcaatgga gaaattgcct
    29521 tgtggacttt ggtgtttttg ccgattttag gaatcgtcat ctggtattac cagaaattca
    29581 gttctcgtat ttaccgaaat atgcgggaaa aattaagtca gctgaacacg aaattgaatg
    29641 aatcgatttc cggcatgcgt attatccagc agtttcgcca agaagctcgt ttagctaaag
    29701 aatttgaaca agtgaacaat gagtatttag acacgcgtta tgcgatgatc aaaacgaatt
    29761 cgattctgtt aagtccgatc atcaattttc tatatgcatt ggcgattgcg ttggtgttga
    29821 ctttgtttgg gatcgatgcg ttgcattctc ccgtagaagt tggactgatc tatgcattta
    29881 cgacctatgt ccagggattt tttaatccaa tgtctcaaat gatggacttt ttgagcatct
    29941 tcacagatgg gatggttgct ggtagtcgta tcttgaagat tatggatacg gaagaatatg
    30001 caccacaaca aaaccctgga gcagatcaaa tgattacttc aggtaagatt gagtttcgca
    30061 atgtaagctt ttcttatgat ggagagcaca atgtattaaa caatatctct tttgttgcta
    30121 atccgggaga gaccgttgca ttagtgggac acaccggaag cggaaaaagc tcgatcatca
    30181 acgtattgat gcggttttac gaattttatg aaggtcagat attgattgat gatcatgaca
    30241 ttcgagaata tccaatggaa gagttacgta agaaaatggg gctggtcttg caagatgctt
    30301 tcttgtttta tggggatatc gcaggaaata ttcggatgat gaatccagct atcacagacg
    30361 aacagatacg ccatgcggct gagtttgttc aagcaaacaa attcatcgat gaattaccaa
    30421 aaggatatca ctcaaaagtg atcgaacatg gtgccagcta ttctagtgga caacgacagc
    30481 tgatcacttt tgcgcgaaca atcgtgactg atcctaaaat tctagtattg gatgaagcaa
    30541 cagcaaatat cgatactgaa acagaaggat tgatccaaga aggattggca aaaatgcgtc
    30601 aaggacggac aacgattgcg attgcccatc ggctgtcaac gattcgagat gcagaattga
    30661 ttttagtttt agacaaaggt catatagtag aacgcgggaa tcatgaaaca cttctagcgc
    30721 aaaatggttt gtatgcagat atgtacaaac tgcaaagcag tggtatataa aagatgttgt
    30781 gacagtagaa ttttgaccta agcactagag tgaaaataaa acatagcttc tcatattttt
    30841 catctgtgag agttagtgtc gtagtcacca gatttaaaaa aagattcgac agctagtcag
    30901 aagtgcctaa tagccactaa gaaagcgttg acaaaagcat ttgctttcga taatataaag
    30961 aaaacgatac aacttggata gtgattgcct gatgcaagct aaacctgatg agtctcccat
    31021 ggtttcatgg acggcttttt gggtttttct ttttgttttg gatacagccc tatacatcat
    31081 ggaaaggagc aaaacatgct gattgagaaa gtactgaata acaacgtcgt aataaccagg
    31141 gatgaaagaa atcaggaaat gatcgtgatg ggaaaaggtt tggcattcaa aaagaaagtg
    31201 ggtgacccga tttttgactc atgcatcgac aaaaaatttc agttgacaaa tgacgggctt
    31261 tcacaacagt tccaagaatt ggcacaagcg attcctcttg agtacttaga aatttcttgt
    31321 gaaatcattg aatatacaaa agaggctcta caagtccagt tgaatgattc tgtgtatatt
    31381 gcattgacgg atcatttgta tacggctatc gaacgtcaca aaatgggaat atttgtacca
    31441 aatgtattgc tgtatgatat caaacaacta tttcctaaag agtatgccat cgggaaaaaa
    31501 acagtgaaga aaatctcgga taaataccac atcaagttat cagaagatga agccggattt
    31561 atcgcattgc atttagtcaa tgcacaaggc attcaaagcc agcaagacaa tacctacctg
    31621 tttactataa caatacagga tattctaaat atcgtccgct attactataa gattacattt
    31681 gatgaagatt cagtccattt ttttcgattc gtcacacact tacgtttttt tctgacacgc
    31741 gtcctagatg gagaaaaatc agaagtggga gaagtttctg aggaattgtt tcaggttatt
    31801 caaaaaaaat ataaggatac gtttggttgc gttgagaaaa tagcagactt tttagccgag
    31861 acgttcaact accagatgtc cacggacgaa aaaatgtatt taacgatcca tactgcgcgt
    31921 ttagtgcatg agaatcgttg taaataaaaa aaggaggata aattaacaag aacgtatgaa
    31981 ataagggtag tgacattcag ttggctaaac cttccactaa taaattggag ggaatagaaa
    32041 tgggaaaata ccaagaatta gcgagaaaaa tcgtagaaaa tgtcggcggg aaggaaaata
    32101 tcaatggttt gacacattgt atcacaaggc ttcgtttcaa attaaaaaac gaagaaaaag
    32161 caaatgatga gatcttgaaa aatatggacg gtattgtaac ggtaatgcga gcgggagggc
    32221 agtatcaagt tgtcattggg aatcatgtgc cagtcgtgtt tgaagaggtt ataaaagcag
    32281 gaaaccttac ttttgatgag gctgtttcaa cagaaaaaat gagaccgttt gatatgctta
    32341 ttgatggaat atcgggctgt ttccagccat tccttgctat cctagctgct ggcgggatga
    32401 ttcgtggact gacagcattc cttgtcttct taggcgcgtt tgaccgcggt tctggcacat
    32461 ttgttatgtt tgataatatc ggtgattctg tattccaatt tatgcctgtt attatcggtt
    32521 taacagctgc ccgtaaattt aaagtaaatg aattcgtagg gatgttgctt ggcgcagcat
    32581 taatgaatcc tagcttgtcg ctggaagctt tgagtggtgc agcagaagca ccactaacga
    32641 ccatattttc tggcactatt tttgaagcac ctatttatca aacagtattc ggcattccct
    32701 ggattgcgcg taactacgct tcttctgtca ttcccattat ctttattgtg ttgttagcta
    32761 gccaaattca gaaaccaatt aaaaagttag tacctgagat gatcgcgaat ttcttcgttc
    32821 cattcttcac cgttcttatt acaatgcctt taggtttttt actcgttgga ccagtattca
    32881 cattcgcaac tgatatttta atggctggat ttgaaacact tttagcactt tcgccagtta
    32941 tttatggagc aatcgtaggc ttcttctggc aaattctggt gatgtttggt ctccattggg
    33001 caatcgtacc aatgggattg atgcaatttt ctgtgaatgg ttggcaaaat attatgacac
    33061 ctgttgcagt agtgtctttt ggacaaactg cagcacttac cgcactctac tttaagctta
    33121 gaaatccaaa agacaaagca attgcaatac cagccattgt ttctggtatt gtggggatta
    33181 cagagccagc tatttatggg tttacattac cacgtaaaaa aatatttatt tttacctgtg
    33241 tcggtggtgc gttagggggc gcgtatagtg ggttaatgaa tttaacttct tggaatcaag
    33301 gtggactagg catctttaca attcccaact atattagacc agatggtgat ttaacagatg
    33361 tgattaatgt attgattggg atcgccattg cgatggtggt atcctttacg ctgacttttt
    33421 tcttttggaa ggatgaagca ggtgaaacag atgatataca gaaaaaatca ggtaaagaaa
    33481 ttgtaaaaac accgatacaa ggacaaatcg ctccactaaa tgcagcaaag gatgcagctt
    33541 ttgcacaagg aacactaggt cgaggaatct tgatctaccc tgaaaaagga gaagtacgtg
    33601 ccccttttga cggaacagtc atgaccctgt tcccgacgaa acaggcaatt ggaatggttt
    33661 ctgaaacagg gctggaattg ctgattcatg tcggattgga tactgtgcag ttggaaggga
    33721 aatattttga aagcttagtg cagcaaggat aaagagtgaa acaaggcgat ctgttgctac
    33781 gcttcgacaa aaagaaaatc gaaaaggaag gatacagttt agaaacacct gtgatcgtca
    33841 ccaattacac ggattatttg gatattttgg aaaaccaagg ggagacggtc gggccagacg
    33901 atgcacttat caccgcactg acctgaacgg ttaggtgaag aatggactag gcttaactat
    33961 tctttttatt ccacttcttt attttcaaaa ttgaaaggct ctttcatgat ggctgagctg
    34021 atagggcgtt tatcgaattt ttgaccgaca gaaattagat ggactaaatt ctgttgataa
    34081 ttttctgcaa aaacacaact atataacacg tctagaagat aacaaattga tgtattgatc
    34141 gtaaaattac caatttttga atacagtcgc tcgcgtgtcg taagtcgcaa agcggctgta
    34201 ctcattgcag atagcgttga atcaccgata ctggtcaaag ctaaaattgg aacatgattt
    34261 tgtttcaata tttctgcggt acgaaggatc acattgtttt cacccgtata agaaatcaga
    34321 atcccgcaca tatcactcgt gcaattgtac gcttcataga atgattcacc aaaagtggtg
    34381 acaatcgtga catccatttt gatgcgcttc attttcagca tgaaatcttg agaaatcaaa
    34441 gtattcgagt tactggcgaa aatcacgatt ttttttgctt gaagcattag ttgtttggct
    34501 ttttgcagtt ggtcatgatg aatgagagaa agtgtatcat gaatcgtcat ctgttctaaa
    34561 gaagccattt tatttgcgat cgtcataata ccgtctgtgg tggaaaaagg aagattcgca
    34621 tctacgtctt gaaaattccc ttcaagatag gctgtttcag ctaaaaaagc attacgaaaa
    34681 ctgacccagc catcaaaacc catttttttt gctacacgga tgagtgtaga ggggtggaca
    34741 taacatcgct cagcaatcgc ttggatggtc atttcgccaa gtgctggaga atcgcttaag
    34801 agaaactgga tcacttcatt ttctgaagga gaaaaggaga tttctttgat tttttcacgt
    34861 aataacatgt agataccttc ttcttatttt tttacgtaaa ttataacttt tttgaaaatg
    34921 atttgtaaac ggatttatac tatataactt aaaatagaca ttttttgaca agatatttac
    34981 aaaaatccac tagaaaacag taaattttgt aaatgttact ggaaagaaac agatttctat
    35041 tgtttgtttt gtttacaaaa aagataatga aagtgtaaaa aagtaaagga gtgagaacat
    35101 ggcaaaagga atcaaaatcg ctacgattgg tggaggcagc agttacaccc ccgagttgat
    35161 ggaaggattt atcaaaagat atgaggaatt accgatacga gaaatctggc tcgtagatgt
    35221 agaagaagga aaagaaaagc ttgagatcgt tagtcagatg tctcagcgta tgtgggatgc
    35281 ttctccgtat gaagtcaaga tctatgcaac tctggatcgg gaagaagcat tgaaagatgc
    35341 ggattttgtt acgactcagt ttcgtgtcgg tcaattagat gcacgaatca aagacgagcg
    35401 gatccctagt tattatggga tgattgggca agaaacaaat ggtgctggcg ggatattcaa
    35461 agcttttcgt accgttccag tcgttttggc aatcgttgag gacatgaagc gtttatgtcc
    35521 aaatgcttgg ctgatcaatt tcgcgaaccc aagtgggatg gtgacagaag cagttatcag
    35581 atatggcaaa tgggataaag tgattggatt atgcaatgtg ccagtgatgg cgatgatgac
    35641 agaaccagca atgcttggca agaaaaaaga agagctgatc tacaagtttg ccggtctaaa
    35701 tcatttccat tggcacaaag tagcagataa taaaggaaat gaccgcacgg cagaattgat
    35761 tgatcgttta tacgatgaag agaatggatt accgaaaaat atctttgata tcccgttttt
    35821 caaagaacaa ttaaaacaaa tgaacatgat cccttgtggc taccaccgct attactatcg
    35881 tgaagaagaa atgctggaac atgctttaga agaatatcaa acgattggta caagagcgca
    35941 gcaagtgaaa gaaacagaag cagagctatt tgaattatac aaagatccag aattagacca
    36001 taaacctgaa caattgcaaa aacgaggtgg cgcttattat tcagacgctg cctgtgaaac
    36061 gattgcttca atctatggaa ataaagaaac acagatcgtg gtatctacaa agaatgatgg
    36121 agctgttcct gacctgccgg cagattgtgt agtagaagtc actgcctatg ttggcgcaca
    36181 aggagcgaga aatgtagcgt ttggttctct tcctgctgct gaaagaggct ggttgcaagt
    36241 aatgaagaat atggaattgc tgacgatcga agcagctgtg actggtgatt atgggacagc
    36301 tttacaggcg tttacgatca atccacttat acgatcagga aagacagcaa aacggatcat
    36361 ggatgagttg tttattgcac accagcagta tttaccgcaa ttcaaggaga caatcagccg
    36421 attaaaagaa gaaggaatcg aagttcaaga tgaactggca agagaattat caggtaatac
    36481 acatataatg gaggaagtat aatgggattc agaaaagatt ttttatgggg cggtgctaca
    36541 gcagccaatc aatgtgaggg cggttatgat gaaggtggac gcggactagc aaacgtagat
    36601 gttgtgccaa tcggaaaaga ccgttttcct gttatcaccg gaaagaaaaa aatgtttgaa
    36661 tttgatgagg atcattttta tccagcaaaa attgcaatcg atatgtatca tcgttataaa
    36721 gaagatattg ctttgtttgc agaaatgggg ttcaagactt atcgaatgtc catcgcctgg
    36781 agtcgtattt ttccaatggg ggatgagaaa gagccgaatg aagctggctt gaaattttat
    36841 gaggatgcct ttaaagaatg ccaaaagcac ggaattgagc cacttgtgac gattacgcat
    36901 tttgattgtc caatgcattt gatcaaagaa tatggtgcgt ggcgttccag agaactcgtt
    36961 ggtttttatg aaaatctgtg tcgagtcatc ttcaatcgct ataaaggact tgtcaaatat
    37021 tggctgacat tcaatgagat caatatgatc cttcatgcac cattcatggg cgcagggctg
    37081 tattttgaaa aaggagaaaa tgaagaacaa atcaaatatc aagcggcaca tcatgagttg
    37141 gttgcaagtg ccatcgcaac gaagattgct catgaagtag atccggagaa caaagtaggc
    37201 tgtatgctgg cagcaggagc atattatcca tacacttgca atcctgaaga tgtttgggct
    37261 tctagaaaat cagatcgaga aaattatttc ttcattgatg tccagtcacg aggagaatat
    37321 cccgcttatg cattgaaaga aatggagcga aatggcattc agatcgaaat ggaaccaggg
    37381 gatgccgcat tgctgaaaga acatactgtt gattttatct ctttttctta ttatgcttcc
    37441 cgagtttcga ctaccgacaa cgaactcttg gaacagactg caggaaatat ctttgcttct
    37501 gtcaaaaatc catatctgca agcaagtgaa tgggggtggc aaattgatcc attaggcttg
    37561 agaataacga tgaatgatat ttatgatcgt tatcaaaaac cattgtttat tgtagaaaac
    37621 ggcttaggtg cagtcgatac gccagatgaa aatggttatg tggaagatga ttaccgaatc
    37681 gattacctgg cagcgcatat ccaggcgatg aaggatgcag tagaactaga cggagtcgat
    37741 ctattaggct atacgacttg gggatgtatt gatttagtat cagctggtac cggtgagatg
    37801 aagaaaaggt acggattcat ttatgtcgat cgtgataacc aaggaaatgg cacattagcc
    37861 cgttcaaaga aaaaatcatt caactggtat aaaaaagtaa ttgattcaaa tggagaaaat
    37921 ctttcgaacg attgatcaat gatttataaa agtctattgt ttttcagcgg atttttcgag
    37981 tataataaag ctattcgaga aacgcaagca gaacaataga ctttttttcg cattggctgt
    38041 aaagtatgct tacttgatta agtaaaggag aatctacata tgtatgatgt aatcgttatt
    38101 ggcgcaggtc ctgctgggat gactgctgct ttatatgctt cacgttcaaa cttatccgta
    38161 ttgatgatcg aacaaggtgc ccctggcggc caaatgaaca atacggcaga aatcgaaaat
    38221 tatcctggat tcgattcgat catgggcccc gatctagctt ataagatgta tgaaagtgtc
    38281 tcaagatttg ggacagaaaa tgcttatgga atcgttcaag atatcaagga tcacggcagc
    38341 cataaagaag tcatctgtga agataaaagt tacgaaggaa aaacagtcat tatcgcaaca
    38401 ggatgtatcc accgtaaatt aggcgtacct ggcgaggaag aatttgctgg acgaggcgtt
    38461 tcttactgtg cagtctgtga tggtgcgttt ttccgtaaca aacgtctagt agtcatcggg
    38521 ggtggggact cagcagtaga agaagccatc tatttgacac aatttgcttc tgaagtagtg
    38581 atcgtccacc gtagagatga attacgtgca caaaagatca tccaagaccg tgcatttgca
    38641 aatgaaaaaa tttcctttat gtgggataca gtcgttgacg aaatcgttgg aaacgaaatg
    38701 gtagtgacag gtgtgaaagc acgtaatgtc aaaaccggca atgaatttga tttagcagca
    38761 gacggagtat tcatctatgt tggactggat ccattaactg caccttttag agaagcaggt
    38821 ttgacgaacg aagctggctg gattccaaca gacaacgaaa tgaaaacgaa tatgccaggc
    38881 gtttttgcca tcggagatgt tcgtgaaaaa gatttgcgac aaatcactac agctgttggt
    38941 gacggcggta ttgctggaca acaagtgtat aaatacttgg agtctttaga agaaacaaca
    39001 gcataagttt aaaaagagta gaagaacggg ttcaattgtt cttttactct tttttttcgc
    39061 gtgaaattta ctgaatctga tttatgcgaa cacgtccaac accttttgat aaaagaatcc
    39121 tttcttgatt taagcgtttt ttggtcctca tactttcata tctaataagt gaatgctata
    39181 cttattaaga accatatttc agaaagaggt gttattttca tgtcttggga acaagtgtat
    39241 gaacaatggg tacatgaaga aaatttagaa gaaaacttaa aaaaacaatt aaatgatctg
    39301 ggtgaagatc ctgaaaaact tgaagatgct ttttatgcac cgttggaatt cggtactgca
    39361 ggtatgcggg gaatcttagg acctgggatc aatcgaatga acatctatac ggtaagacaa
    39421 gcaacagaag gactagcacg tttcatggat acacaaggtg aagaaacaaa acgccgaggt
    39481 gtcgctatcg cttatgactc acgccatcag tcaccagaat ttgcaatgga agcagccaaa
    39541 acactagcac atcacaatat cccgtcttat gtattcgaaa gtctgcgtcc aacaccagag
    39601 ctttcttttg cagttcgtca tttgaagacg tttgcgggaa tcatggtaac cgcatctcac
    39661 aacccagcgg cttacaatgg atataaagtc tacggagaag atggcggaca aatgcctcct
    39721 gcagacgcag atgcattgac gaaatatgtc cgtgaagtca gcaatccgct aaaagtcgaa
    39781 gtattatctg acgaagaagc taaacatagc gggttgatca cgatcatcgg agaagaagtg
    39841 gacgctgctt atctagaaga aatcaaagca gtgacgattg atcgtgaatt ggttgaaaca
    39901 atggggaaag atttgaaact tgtgtacacg ccacttcatg gaacagggaa aatgttgggc
    39961 gaacgtgcgt tgaaacaagc tggattcgaa caattcgtac tagttcccga acaagctgtg
    40021 gcagatccag atttcactac ggtcaaatcg ccaaatccag aagaacattc agcttttgaa
    40081 tatgctatac gacttggaga aaaagaagga gctgacttgc tgattgcaac agatcctgat
    40141 gcagaccgtt taggcgcagc ggttcgtttg ccagatggtt cttatgaagt attgactggg
    40201 aaccaaatcg gagcattgtt tgctcaatat attttggaag cacatcaatc aagaggcact
    40261 ttaccagaaa acgcagcggt attgaaatca atcgtttcca gcgaattgcc tacagcaatt
    40321 gcggaacact acggtgctac catgttcaat gtgttgacag ggtttaaatt cattgctgaa
    40381 aaaatccaac aatttgaaga agaccattca catactttta tgtttggatt cgaagaaagt
    40441 tatggttatt tagttaaacc ttttgttcgc gataaagacg cgatccaagc attgctttta
    40501 ttagcagaag tagcagctta ctataaaaaa caaggcaaaa cgttgtatga cggcttgcaa
    40561 acgatttttg aaacctatgg ctatttcgca gaaaaaacga tttctgtgac aatgagcgga
    40621 caagaaggtt ctgcgaaaat ctctgctttg atggaaaaat tccgtaaaga agcaccgaca
    40681 gaatttgccg gagcaaaagt tattcaaaca gaagacttta aagaattgac gagaacagac
    40741 cgtgaaggaa acgtagaaaa actaacaaca ccgccatccg acgtattgaa gtatacattg
    40801 gaagaaaatg gctggatcgc tatccgccct tcaggaactg agccgaaaat caagttttat
    40861 attggtgtaa aaggaagctc tacagaagac gcagccgaaa aagtagcctc ttatgaagcg
    40921 attatcaaaa agatgacaga agaataaaaa acttgagact gatggaaaaa gtggtaaact
    40981 ttagaagtca gtcttttttc aagttggagg tgaatggatt gccagacacg caaaaatcaa
    41041 aagtagagaa agtcagtcaa ttattcaaaa tgttgagtga tcccactcgt cttaaaattc
    41101 tgctatatct gaaagacggg gaacaaaatg tgacagcaat cacccaagca gtggaaatgg
    41161 agcagtctgc tgtatcgcat caactacgtt tattaagaga aaatcatgtc gtcaagtctc
    41221 atcgcgaggg aaaagcgatt ttgtatagtc ttgatgattc tcatgtactg gatattttga
    41281 accagacact caaacatgtg gaacatcaat aacgataggt gaaaatagat aaacactgga
    41341 gtgtgacaac gaagaaattt cgttgtcaca ctccagtgtt ttttaagaaa aagacaaaaa
    41401 tgattaatcg cttttttctg ttgtttcagt tccataataa ctatgatctt ctaggtctaa
    41461 agcattcaaa ataagcgaag ccgtttcttc aatagataaa gatgccacat tgatcactac
    41521 acatcctagt ttttcataaa gatcattcgc aaaatcgagt tcagctctaa ttttttcgat
    41581 atcggaataa gcagtatcag ggtttaatcc gtaagagcgc atccgttctt tacggatatt
    41641 atttaaaata tctgggttat tcgttagtcc cacgattttt ttaggatcga tttcccacag
    41701 ctgtttggga atatgtgctt ggggaatcaa aggtaaatta gcaactttta aatttttatt
    41761 tgctaaaaac aagctcaaag gcgttttgga agttcgggat acgcctagca aaacgacatc
    41821 tgcttctaaa aaaccacgag gatctttccc atcatcgtat tttacagcaa attccattgc
    41881 tttgatccgt ttgaaatagt tctcattcaa taaatggagg gcaccaggtt gccgaattgg
    41941 cttgatacca gttaaacgtt caatttctga tatcggtggc gtcatcacat cgaaactaaa
    42001 taattgatgt tcttcacaaa aagaacgtgt tatctcgatc agttcttgat tgattaatgt
    42061 atgcaggaca agtgcttttt cttctaatgc atcttgcaat gcctgcctta atgttttttc
    42121 atctgtcacg aacgttctgc ggaataggtt gaactcaacg gtaggatact gagccatagt
    42181 agcttgcgct agttttgtcg cagtttctcc agcagagtcg gagatgacaa aaaaagtaac
    42241 gatttctgta gaatcggaag acatggataa aacctcactt gcattttttt tatgtttatt
    42301 ataccatact ctagaatgcg tacttcgtaa tcattttgat ttggaggaat aagtcatgaa
    42361 aaaacagaca ttgatcgaac aatcattaga aacattgaaa aataacggtt ttaaatatac
    42421 taaaaaaaga gaagcattgt taacttattt agtgaagcgt aaccgctacg tatctgctaa
    42481 agaagttttt gactatatga atcaggaatt caaaggggta agttatgata cgatttaccg
    42541 gaatctccat gattttgcag agatcgattt actggaagaa accgatttaa atggtgaaat
    42601 gaagtttcgt tttcattgct gtcaggggga aatcggccat caccatcatt ttatctgtac
    42661 agtgtgcgga acaacgaaag aaatccatat gtgtccaatg aattatttcg aagaacagct
    42721 aggtggatgc acgatcgaag ggcatagatt cgagattttc ggaagatgtg aaaattgtca
    42781 ataaggattt aaataaaata acatttgata atttaaaaaa aaagcaagat ttccttgacg
    42841 gtcaaaacag cattagatat aatatttaca ggacagtctt gttttttgag atatatttca
    42901 ataacaatta cggttacttt ttaagctcgg agggagggaa ttaacatgtc aaaaacagtc
    42961 gttcgcaaaa acgaatcact tgacgatgct cttcgtcgct tcaaacgttc cgtgtcaaaa
    43021 gcgggtactt tacaagaatc tcgtaaacgt gaattctacg aaaaaccaag tgtaagacgc
    43081 aagaaaaaat ctgaagcagc tagaaaacgc aaaaaattct agttctgtga tgaattggag
    43141 ttggtagtgt gtcactacta agtagattaa acgatgacat gaaagcagcg atgaaagcga
    43201 aagataagga atctttacaa gtcatccgaa tgatcaagtc atctatacaa aacgagcaga
    43261 ttaaagaagg tcacgacttg acagaagaag aagaactaac ggtgttgtct cgcgaaatga
    43321 aacagcgacg tgattctttg catgagtttg aagaagcagg acgtgacgat ttagctgaaa
    43381 aagtaaaaag tgaaattgta atagtcgaaa aatatatgcc ggaacaactt tccgacgaag
    43441 aaattcgaca acttgttcaa gaagcaatca ctcaaactgg tgcttcatca atgaaagaat
    43501 ttggcaaagt tatgggtgca attatgccta aagtcaaagg aaaagcagat ggaaaccaag
    43561 tgaatgcgat tgtgaaagaa ctattacaat cttaat
//
LOCUS       DAZULY010000002        42308 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000002 DAZULY010000000
VERSION     DAZULY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.002"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1695)
                     /locus_tag="ACG81A_000040"
     CDS             complement(<1..1695)
                     /locus_tag="ACG81A_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013451.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLR"
     gene            2202..3362
                     /locus_tag="ACG81A_000041"
     CDS             2202..3362
                     /locus_tag="ACG81A_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5013452.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            3378..3821
                     /locus_tag="ACG81A_000042"
     CDS             3378..3821
                     /locus_tag="ACG81A_000042"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5013453.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(4304..4834)
                     /locus_tag="ACG81A_000043"
     CDS             complement(4304..4834)
                     /locus_tag="ACG81A_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFS5013454.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            4939..5490
                     /locus_tag="ACG81A_000044"
     CDS             4939..5490
                     /locus_tag="ACG81A_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5013455.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(5643..6116)
                     /locus_tag="ACG81A_000045"
     CDS             complement(5643..6116)
                     /locus_tag="ACG81A_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5013456.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            6254..7726
                     /locus_tag="ACG81A_000046"
     CDS             6254..7726
                     /locus_tag="ACG81A_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS5013457.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            7723..8433
                     /locus_tag="ACG81A_000047"
     CDS             7723..8433
                     /locus_tag="ACG81A_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFS5013458.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            8785..9069
                     /locus_tag="ACG81A_000048"
     CDS             8785..9069
                     /locus_tag="ACG81A_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFS5013459.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(9350..9769)
                     /locus_tag="ACG81A_000049"
     CDS             complement(9350..9769)
                     /locus_tag="ACG81A_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFS5013460.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(9771..10355)
                     /locus_tag="ACG81A_000050"
     CDS             complement(9771..10355)
                     /locus_tag="ACG81A_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFS5013461.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            complement(10402..11877)
                     /locus_tag="ACG81A_000051"
     CDS             complement(10402..11877)
                     /locus_tag="ACG81A_000051"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFS5013462.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRNIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            12058..12369
                     /locus_tag="ACG81A_000052"
     CDS             12058..12369
                     /locus_tag="ACG81A_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013463.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(12484..14820)
                     /locus_tag="ACG81A_000053"
     CDS             complement(12484..14820)
                     /locus_tag="ACG81A_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFS5013464.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            14962..15630
                     /locus_tag="ACG81A_000054"
     CDS             14962..15630
                     /locus_tag="ACG81A_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5013465.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            15633..16676
                     /locus_tag="ACG81A_000055"
     CDS             15633..16676
                     /locus_tag="ACG81A_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5013466.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(16913..17794)
                     /locus_tag="ACG81A_000056"
     CDS             complement(16913..17794)
                     /locus_tag="ACG81A_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5013467.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            17953..18681
                     /locus_tag="ACG81A_000057"
     CDS             17953..18681
                     /locus_tag="ACG81A_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFS5013468.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            18837..19154
                     /locus_tag="ACG81A_000058"
     CDS             18837..19154
                     /locus_tag="ACG81A_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5013469.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            19147..19755
                     /locus_tag="ACG81A_000059"
     CDS             19147..19755
                     /locus_tag="ACG81A_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFS5013470.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            19810..20601
                     /locus_tag="ACG81A_000060"
     CDS             19810..20601
                     /locus_tag="ACG81A_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFS5013471.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            20739..21251
                     /locus_tag="ACG81A_000061"
     CDS             20739..21251
                     /locus_tag="ACG81A_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFS5013472.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            21277..22149
                     /locus_tag="ACG81A_000062"
     CDS             21277..22149
                     /locus_tag="ACG81A_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS5013473.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            22325..22687
                     /locus_tag="ACG81A_000063"
     CDS             22325..22687
                     /locus_tag="ACG81A_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5013474.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            22696..23448
                     /locus_tag="ACG81A_000064"
     CDS             22696..23448
                     /locus_tag="ACG81A_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5013475.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            23462..25264
                     /locus_tag="ACG81A_000065"
     CDS             23462..25264
                     /locus_tag="ACG81A_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5013476.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            25541..26581
                     /locus_tag="ACG81A_000066"
     CDS             25541..26581
                     /locus_tag="ACG81A_000066"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS5013477.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(26633..28777)
                     /locus_tag="ACG81A_000067"
     CDS             complement(26633..28777)
                     /locus_tag="ACG81A_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5013478.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(29134..30507)
                     /locus_tag="ACG81A_000068"
     CDS             complement(29134..30507)
                     /locus_tag="ACG81A_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5013479.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(30507..30902)
                     /gene="rbsD"
                     /locus_tag="ACG81A_000069"
     CDS             complement(30507..30902)
                     /gene="rbsD"
                     /locus_tag="ACG81A_000069"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFS5013480.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(30930..31847)
                     /gene="rbsK"
                     /locus_tag="ACG81A_000070"
     CDS             complement(30930..31847)
                     /gene="rbsK"
                     /locus_tag="ACG81A_000070"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFS5013481.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(31955..32968)
                     /locus_tag="ACG81A_000071"
     CDS             complement(31955..32968)
                     /locus_tag="ACG81A_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFS5013482.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(33049..33648)
                     /locus_tag="ACG81A_000072"
     CDS             complement(33049..33648)
                     /locus_tag="ACG81A_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS5013483.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            33834..34535
                     /locus_tag="ACG81A_000073"
     CDS             33834..34535
                     /locus_tag="ACG81A_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5013484.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            34545..37256
                     /locus_tag="ACG81A_000074"
     CDS             34545..37256
                     /locus_tag="ACG81A_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFS5013485.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            37653..39281
                     /gene="treC"
                     /locus_tag="ACG81A_000075"
     CDS             37653..39281
                     /gene="treC"
                     /locus_tag="ACG81A_000075"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFS5013486.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(39341..40522)
                     /locus_tag="ACG81A_000076"
     CDS             complement(39341..40522)
                     /locus_tag="ACG81A_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5013487.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            40774..42297
                     /locus_tag="ACG81A_000077"
     CDS             40774..42297
                     /locus_tag="ACG81A_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS5013488.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
BASE COUNT        13515 a         7556 c         8524 g        12713 t
ORIGIN      
        1 acgtaaaagc ttgtatctat gataaataaa taaacatagc aaggtaaacc agccaatcaa
       61 agtaagagaa agaatgaaat agaaatagat tggcgtttga taatgaagcg tgagctcgtt
      121 gattcctttt ttctgcttca cgattggggt cccgatatct gttacttcat aatcagtcaa
      181 tgtttttcca ttttgctgta gcacagtccg atcgtagacg ataacaggga tatgaacagt
      241 tttgtcccca gtgtttttcc acttgattgt caaaagaccg cctggatgtg tttttataaa
      301 agttgtttcg ttttctagta tttcttcgtt ataccggtcg tatttattgt cttttgtctc
      361 cttatagatt ggcagatagt caggtgtggt tttttggaaa acgaacaagc tggtagacat
      421 atctttgctg aagaatgttt tccttgcttc ttccggcgta tcgaatagat aagtatgtcg
      481 actgacaaat gtttcatttt cccacttttc cagatgctgg tataagttct ggatcgtctg
      541 tgctgaaaaa ataagaatgc caaatatact taaaagacgg aaccactttt gttcagacca
      601 ctggttcagt gaaagggcaa ggtaaagtag aagcaatagc gtaaatggca cgaaaaatcg
      661 aaatggaaac tggatcaaac taataaaagg cacatttttt tcgatcaatg tcgtccatgg
      721 aatcaagcta gtagaaagaa aaaagaaaac aagacttgtg tacaacacga ttttttggaa
      781 taaggtgttc ttttgttgaa agaaaataaa aagcaaaaaa cctgataata tcaatatagg
      841 aaaggcctta gggtaatgga gccaataact gccatccaga tctatcgcct tttgactcat
      901 ttctctgtta acaaagggag gaagaatctt attcccactg taaacaatag ctaatcctgc
      961 ccaaatattt gctgtcagaa caaaataaag caagaccgac ttaatcagtt gaagcagtgc
     1021 ttttcctttt tcagtctggt taaaaaagta ataaagataa aaagggatat acatcaaagc
     1081 taacatgatc gaagacagta aatggatctg tgtcataagt cccactgaga cagcgacttt
     1141 gataacaggg aactttttat gaaggacgaa atcagcaata gggatcatac ataaaggata
     1201 aaaacaagtc ccccagctag tgaatccttg gttgatagac cagtattgaa tagaatacgt
     1261 actcatgaat aaaacggcta ctggtacact tacccaattt cgaacattga catatctcag
     1321 taaaatcagc atagaaaatc ctgaaataag ataaagaatg aaatttgaaa gaatctgata
     1381 atgaaaccag ctacctgcaa gcaacacgat caatccttgt aaataagcga aaaatgggcc
     1441 gtaaacggca ttcacgatcc tgccactttg ctggaatcca taaagggaag gaaaataatg
     1501 gaaatcccca tatttaattt gttgcgcggc atcatagaac cgattgtaat ggaatggggc
     1561 atcagcaccc aaaattagtt tatgtgtgaa taaatgaggc atgagaaatg ccaaagcaat
     1621 gaaaaaaatt gctaaaaatg ggccatattt tttcaattgt cgaactcctt tgcaaacttt
     1681 agataaattc atcatatatc ctatgcgaga ggtaggcaag aaattagaaa attcatgaaa
     1741 atatttatga attttacaag aaaaacataa gtgaaagttc taaggagaga ggatgcgaca
     1801 taagttttgt tgcatctcct tgttttgaaa cagaattttt cttaatactc gaaacaaagt
     1861 agctttttca tgacctctcg gtaaacggtg ttcaatcagc tgcttctcgg taataagctc
     1921 actagcctag aaaatatgag aagctgtttt ctgactagcg ctcttattac tcaaagcata
     1981 acgcagtttt cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa
     2041 gcccaaccca acaaaaaata tgagtagcta tttttttggg ctgtacttat tacttgatgc
     2101 aggacagctt tttcacaacc tcatcggctt tcattttcta tttttttcct gaacccatat
     2161 aataaaagaa agaacaaaaa aacttgcaag ggaggacaca tgtgaagcag agtaagcaaa
     2221 aacagtctcg tcgacttttg tttcgtttgt ttttattaat atgtatcgtc gtagggttac
     2281 tgatcgcttt atatccattt tatgtggatt ctttaaatag ttttattgat caaaaacgta
     2341 tcgaacaagt acaaaaacgg accgcagctg aaattgaagt gcagcgaaag aaaatggagg
     2401 agaaaaaaca acgattaact gaccaaggat tcaatcctgg tactgatcct ttcaatgaaa
     2461 agaacagaaa tgaaagtaca acatccagcc aattagagga atggctgatc ggaagtatca
     2521 atatacctaa gatccaatta aatatttcac tttatgatcg gctcaatggt aggatccttg
     2581 aaaatggagc aggcgtcttg caaggaacct ctttcccgct tggtggaaat tcgacgcatt
     2641 cagtgatctc tgctcacagc ggtttaccta atcgacggtt atttaccgaa ttagatcgtt
     2701 tagaaaacgg agacacgttt attttgactg ttttggggga gaaattggct tatcaagtta
     2761 aaaatataca agtggtactg ccagatgaca cgtctgttct aacgatttca gaaggagaag
     2821 acttagttac cttgcttaca tgtacgccgt atatgatcaa cacgcatcgt ttgctagtga
     2881 caggacatcg gattccttat aacgaatcgg taaaaaaaga agaaggaaaa ggaaatcagg
     2941 aaagaaccat gcggcaatgg tttattcttg caggaacgat aatagctgta gtcatactgt
     3001 tagtgtttat cggacgacta atctatcagt atagattaag taaaaaagtg cttgattttt
     3061 catttattat tagtgattcg tcagggaaac cggtaaatgg tagttcattc attttgaaac
     3121 ataagaaaaa aacacttact agaaatggtg tgccgttttc agtacaaagc gaccatcatg
     3181 ggaaagtcaa gctggatcaa ttgcctggtg gaacttatcg aatcgtttca gctgatgatc
     3241 ctaaagttgc tgtttctttt ggtattagaa aagtaaaaca agagaagata tacttttttg
     3301 aggggcgaaa acttgtaaaa gagcttcaaa aaaatggttt ttattttaaa atcaatgaat
     3361 aagtaaggag agaaaaaatg atccgcacaa caacaaaatt gtctgccaaa gaactggaat
     3421 ctgttctatc gatttggctg catgctaact tggattctca tgaattcatt ccatcaactt
     3481 attgggagaa tcaagtagaa tctgttagga aagctttacc agatgcagtg atttacttag
     3541 cagaaataga tcaagaaatt attggttttg ctggggtgat tgatcagttt atcgcaggaa
     3601 tctttgtgaa aagggaatac cggcaacaaa gaatcggtac gcaactaatg aaaaaaataa
     3661 aagaaaccaa taaaacactg actttgacag tctatcaaaa aaatcaatca gctattcatt
     3721 tctatctttc acaaggtttt acaattcaaa cacagcaaaa agatgaagca gtaaatgagg
     3781 aggaacttgt gatgaaatgg aatcaaaacg ctgatttttg atgaacagaa taacgaaata
     3841 atgctcccag cctaattttt tgctgggagc attatttcat tttgaagtca ctaatttaac
     3901 tgtgtgaata tgcagttgtt acaatctttg ataaacggtg ttcaatcagc tgaccctcgg
     3961 tattagctca aaaaacggca aaatatgagg aactattttt ccatttgttt ctctaatact
     4021 caggtcaaaa cgctgatttc acaacctctt gataaacggt gttcaaacgg ctgcttctcg
     4081 gtaataagcc cactagccta gaaaatatga aaagctattt tctaactagc gttcttatta
     4141 ctcaaagcat aacgctgatt tcacaacctc ttgataaacg gtgttcaatc agctgaccct
     4201 cggtattagc tcaaaaaacg gcaaaacatg aggaactatt tttccgtttc tttctctaat
     4261 actcaggtca gaacgctgat ttcacaacct ctctttcaca accttatttt actcgtttat
     4321 aattttcatc gaagtatttt ccgctacgga tatcatctgg cattccatcg tatggttctt
     4381 cgctaataac atcttcattg tctatacctg catcacccat ataagtgatc gtagcaaatt
     4441 caggaacaag taattttgga taagtttcat atttttcacg agctgcttcc atttctgcga
     4501 tatgttcgtt ttggaaagta acagtgattt ctggatgttc ttcaacccat ttagggaaaa
     4561 agatcattcc gtgaagtttc ttttcatttg gcataaagtc caatgccacg tctgtccctg
     4621 ttggctctgt ccaagtgatt ttgaatacgc cgtccgtcaa tttaacgata tcagcttctt
     4681 ggtcttttac ccaacggcct ttgaccattc cgccatggat acggtaatca acagtatggt
     4741 catttttggc ataccattca tattcccaac cattgtcata cgtatagata aagtgagttc
     4801 caataaaatc ttccaattct ttaaattctt tcatgtttaa gcatctcctt ataaattaaa
     4861 acatgtaata aactacatgt tttcgattac tcatttatga tagaatggat ttaaagattt
     4921 gtcaaggagg gaaaactgat ggcaagaaaa aatacacttc gatatatttt gttagggtta
     4981 ttaagtaaga agaagatgac aggatatgaa ttgaatcaat cgttcaaaaa tgaaatagga
     5041 gaattttggc aagcaaaaca tagtcagatt tatcctgaac ttgcaaaaat ggaagagcaa
     5101 ggaatcattc agcatcaagt agagatcaca ggtgaaaaat tggaaaagaa agtttatgaa
     5161 ttaacagaag aagggaagga acaattggca gagtggattc atacaccaac caatgaacta
     5221 ccggttaatc gagacgaatt tgttctgaaa ttgtatttcg tcaaagacgt gaatgatccg
     5281 gcattagtag aaattattca gcagcagcgt gaccttcatg aagaaaaacg acttcatcta
     5341 ttagagcgaa aaaaaacgat ctttcctact gaaaaagaaa aacatgaaaa ttacggacac
     5401 tacctgattt tagcgcatgc gatcaatcgg gaaacagaat atgcaaattg gctaacagat
     5461 gtattagaag aaagtaaccg aaaaaaataa aaaaatatcc gaacagtgat ggagaattcc
     5521 tttctatatg aaagaaacgc agatcaccgt tcggatattc ttatgttcaa acaggccttt
     5581 tttcaagtat ttatgtagca tctacttatt aaaaagcttg gtacttagga gattagtggc
     5641 gtttagagtt cttttaacaa ctgttcgtag tcagcttttt catcgacagg aaggagcttg
     5701 taagttaatt cgtctgtctc gttgtcatga atgatgtaca tcttagcatt ctcttctttg
     5761 aagttaccga tttgtaaaat cgtatagcgt tcgtcgatca attcgatatc tttgattgct
     5821 tttgaaaaaa ctttacggac atggtgcatt ttcttttggc ggatttctgc cttttctgca
     5881 gcttttgaca aagtaaatcc gtcatctaaa tattttttga tcagttctac ttttatgact
     5941 gtaggtaaac gatattttct aggacaattc gcatcagctt cgatcgaacg aatgaatccc
     6001 ttttgttccc aataccttaa ttgacgtgga gacacaccta cgatatcact taattcactg
     6061 atgccaacaa ccaattgatc attttccaac aaacgtttaa gggaagcccc aaccattttt
     6121 gtcactcctt ttgtcctctt gcttccctta attatatccg tattaactta tgtgtcaagt
     6181 aggttgacaa atagcgaatt taggactaaa gtattctagt agtttcagaa aaaattcaga
     6241 aagaaggaga aatatgagta acaaccaacc cgtagatatt tacggaaaac catataatcg
     6301 gacactttta gttgttgtct tattaattgg tacattttgt accgttttga atcagacctt
     6361 attgacgaca gcttttccta cattgatgaa agctttcgat atttcagcgt ctgatgtgca
     6421 atggttaacg actggtttct tgctagtcaa cgggatcatg atcccaataa cagcatggct
     6481 gatcaataaa ttcagttcaa gaaatttata tctttctgcc atgacgatct ttttgatcgg
     6541 aacgattact tgctttacag cacctaattt tagtacatta ctaatcggtc gcttgatcca
     6601 agcttgcggc gtgggagtat cgatgccatt attgcaaaat atcatgctat ctattttccc
     6661 gccagaaaaa agaggatcag caatggggat gtcagggatc gttattggac ttgctcctgc
     6721 tttaggccct acattatctg gttatatcat tgataactac cattggcgtg acttgtttgg
     6781 tatggtactg ccaatcgttg ttcttgtctt ggttttagca ttcttcctaa tgaaaagtgt
     6841 tattccattg tcaaatccta gcattgatat tttatccgct attttatcaa cgattggatt
     6901 cggtagttta ctttacggat tttctagtgt aggagatgac ggctggggaa gcttgaaagt
     6961 aattggtttc ttagttgttg gtgtgatttt catcgccttg ttctcatggc gtcaattaca
     7021 tttggaacat ccattcttgg aacttcgtgt attcaaatca tctacattca cgattgcagc
     7081 tgttttagct ggtgtaacaa atatggcgat ggtaggggca gaaatggtcg taccattgta
     7141 tattcaaaat attcgtggag aatcagcatt ccattctggc ttgatgttgt tgccgggtgc
     7201 attgatcatg ggggctatga tgcctgtgac gggtgcgatt ttcgataaat acggcgcgaa
     7261 acgtttagcc atcacaggga tgttcatttt gacagcggca actgcaccat ttgctttctt
     7321 gacaaaaacg actccagttt tatatatcgt gatcctttat gcgatccgta tgtttgggat
     7381 ctccatggtc atgatgccgg taacgacttc tggtatgaat gcattgccaa ataacttgat
     7441 cagtcatggg acagcggtaa ataatacatt ccgtcaaatc gcaagctcga ttggtacagc
     7501 tattttgatc agtgtgttga cgaatgtgac aaaagcaaat atcccaagca aatctgtatt
     7561 ggataatacg ccattagctt ataaagatca ggcaatcaat gccacactgt caggttacca
     7621 tgccgcgttt atcgtagcga tcatctttgg gattcttggc tttattattg cctttttatt
     7681 gaagaaaaaa gaacctgttc taatgaaagc gggggataat gcatgattat tggagttttg
     7741 attgtcagct tgcttgcttt tgctttaacg aacgtgttcg ctaaaaaaac atggcaaacc
     7801 tttttatctt tgattttcgg actaatcttc gtcgcttcac taagtttgat cgtcgctaat
     7861 ttgtccaatc actttggaat ggaaaaagta acggaaacaa aaacagaaaa aattgtttcc
     7921 agtgctgatt cccaaggagc agatatgctt ttgtataaag ctttagggaa tggaaaagaa
     7981 aaagtgtatc tttaccgaac aaatgaaaaa caagaaaaac caaaagcaac tggtacggat
     8041 aacgaaacaa ataaagtaga aaagacagac ggcgatgctg aaaaagtgac gaaaactact
     8101 tattgggaat acaaaaacga catgtataaa ttctggttca atattgctga taacaatcac
     8161 gaatatgaca gccgagtgaa tacattcaaa atcccagaga catgggtaga attaagtacg
     8221 gaccaagcag ccaagttagc tgaattagtg aagaaacagc aaagtacgat ggaaagtgaa
     8281 gcaaaagctt atgttcaaga cggtatggta aaagcaatga ctgaaaatcc aaaaatgagc
     8341 aaagcagagc aagaacaacg tacgaaagcc ttagctgctg aattccaaca acaagcattc
     8401 gcaaaattag tgaaagaagc aaaaggcgaa taatattttg aacaccatgt aggaggttgt
     8461 gaaaaagctg ctctgcatca agcagtaaga acaatcagat aaaaatagca ctccatattt
     8521 ttcatctgat tgggcttagt gccgtagatg caagcttttg aacaccgttt aaggaagagg
     8581 ttgtgagcct gccgtttaac tctgagtcac aaggagcaat ttcaaaaaac agcttctcat
     8641 gtttgttgac tattgtgact tgtgaccgag gagttggcag gagaacaccg tttattcgaa
     8701 aaaaggaact gcgacaagac ttttgtcaca gttccttctt ttttttgcta tgctatacta
     8761 gatgtaaaaa ataggagaaa taaaatgagg gaaatttatt cttttgaatc aaaaatctat
     8821 gcttccgaag tcgacaaggg cggggcatat attatttttc cgtatgatat aagaaaagaa
     8881 ttcggtgctg gaagagttaa agtccaagca acttttgaca atatgccgta tgaaggcagt
     8941 atcgtgaata tgggcgtgaa aaatccagat ggttcagtct gctacatcct tggactacga
     9001 aaagatattc gtaaaaacat cggaaaagaa atcggtgata ttgtagcagt tacagtgaag
     9061 caaaaataaa agaggttgtg acaaaagacg tgtcacaacc tcttgataaa cggtgttcaa
     9121 aagccagttt ctcggtagta agtcaaattt atgcaaaaat gtggaaaccc atgtttacaa
     9181 aatttttctt actactcgaa acgaagcggc tttatcacaa cctcttgata aacggcgttc
     9241 aatcagctgt ccctcggtat tagctcaaaa acggcaaaat atgagaagct atttttccat
     9301 ttttttctct catactcagg tcataacgct gatttcacaa cctcttcttt cataatgttt
     9361 tttcaccatc tttataataa actggttctt tgatatggaa atctattttt tccaaccaat
     9421 ctaaaatctt gtaacctaac tcttctgaca tcggaaaaga ggcgttgatt tgtgcattgg
     9481 agatatggtc aacatggata ccggaagtga tcgtacaact tcccggaacg gcagttttga
     9541 tgatcacagc tattttttcg gctaagacat cgtctttatg gaaccgacca tcgtggctag
     9601 ggaaacgtac agtttgcatc tctgtatcac gagaaaatgt agtgactgtt ccgatatgtg
     9661 gtgtatctcc gcctgttaaa gtgatcaata agtctttacc gattacttta acagcagctt
     9721 ggatcgtgaa acctagtttg gttacttcaa ctatcgtttc tttttccata ttaatcaccc
     9781 tgccaacgtt ttgaatgctc atttacaatt ccaaattgat ccaaaagttt cattgtatgg
     9841 tgattaacaa gatcttggat cgtttgaggc tgattataaa aagcaggaat tggtggtatg
     9901 atttgcacac ctagtcgtgc taatttagtc aagttttcca agtgaatggc atttaatggc
     9961 gtttcacgag gaacaatgac taactttcgt tgctccttca ttgttacatc agcagcacgt
    10021 gcaatcaggt tgtcgcctaa accaacagag atacctgcga ctgttttcat acttgccggt
    10081 acgatgatca ttccatcggt caaaaatgat ccgctggcga cagcagcacc taaatctttg
    10141 tttgaataat ggtaatcaaa caggctttcg aattcagtta gtgataagtc ggattccagc
    10201 tttaagttct gttttgccca tggactgatg attccgtgtg tttctatcat cggatattct
    10261 tttagtttct tcactaggtt gatggcataa atggtgcctg aagcgccact tacaccaatc
    10321 acgatttttt tcttccgttg tgccaattgt tccattttcc gcactccttt ttatgatgtc
    10381 aaaacagttg cagatcattc attatttcaa atatttttcc caatcttcta cttcttggaa
    10441 ttttgcacgt acaaaatgtt ctttcatatc gaaaggaact gtaccgtcaa agatagtttt
    10501 tgcactcatc ccacggaaac gaatagagcc aggattatat tctggtaatt ctgatgggtc
    10561 caatggatgg ttcctcattc ctgggatcac gatgatatct ttatctcctt ggaaacgagt
    10621 gttgaaagtc caaactacat cgttcatgtc gaaaatatca acatcttcat cgactaaaat
    10681 gactgtcttc aattctttga aggctgataa agcaagaata gctgcttgac gctgaatacc
    10741 ttcatccgct tcgttttctt tacggatttg catgatggtc atcaatttac caccacctgc
    10801 aggaggattg tagacattta ctacttttcc tggaatagct ttatcgacca aggcaagaat
    10861 acttgcttct gtaggaatac cggccataga tacgtgttct tcactgggac caatcgttgt
    10921 ttgcatgatt ggattgtctt ttcggtgagt aactgctttg actttcacta cttgtacagc
    10981 tggattagca tccccagtat agccaggaaa ttctggcatt gcttttcctg agttcgtgtt
    11041 gatatcttct tgcattgttt catttggcat gatttctgct tcaatgatga attcagcacg
    11101 agcaataccc acttcatcga ctgctatacc atcaacgatt tgaataggtt cgttacgcaa
    11161 agctcctgca caccatagtt cattgtagcc aagtggagtg gtaggtggtt cgaaagtcgt
    11221 gccaatggca attgctggat ctaaaccaat attgatagtg ataggcattg gtttattcaa
    11281 ggcttcgaat tgtttttgga agtgaccaat gttgcgtccg ccaggcatga tgtacatacc
    11341 gatcgtatct ttatcttcta gaaccatacg atggatcgtt acatcgctca ttgttttttc
    11401 tggatctgat cctaagacta cacccattgt gatgtatgga ccagcatctt caggagtatt
    11461 tgtaggtgca ggcaaaatgt tgcgaatatc gaagtctgca tcggtagcta gatgaacgac
    11521 ttcttgtgct ggagcgtctg ctttgtctac tttcacaggt ttgattggat tttctacagc
    11581 gtctttcaag aaacgaccaa gtgttcgata atcatggtgc aaaatttcac ctacacgttt
    11641 acgactagcc attgttccga tgttcacacg tgtgccgggg aaacctttga tattgttgaa
    11701 agtcatagct ggtccttctt gagtcggacg ttttactgta ccgccagcac cgatataacg
    11761 gtaaacacca gataattctg cttcaggatc gatttcaata tctgtttcgt gatattgacc
    11821 tggaagtgtt tttagttctt cgatgacttt tcttaaatca tacggttgat ctgtcatgag
    11881 agattcctcc ttagttgtct ttacctatct atagtaaaag gtgaaaaaga ggaaaacaat
    11941 tcaacttgtt cggtaaagta ttctacaatg gaatggatgc gtttttttga taatagaata
    12001 aactatgata tacttatcac taaaagaaag cgcatactta gtgttggagg aaggaaagtg
    12061 aaaaaaatga aggagtcatt tcctaaagca gtaaaagtgg aaaatatagc gaatattttg
    12121 aaagttacat ttgaaaacgg agaagtaaaa tacgtgaaat ctcattggac agaagagata
    12181 accgatgcct tgcaatttgg aaaaaaagga agaggaaaaa gaaaaaatct tctcgcactt
    12241 tctcgtaata tgtggattgg gactgaagta acgattgaag cagatggaac agtattcata
    12301 aatggaaaag acaggtatac gccagaagag ttgtggtaca aaggaaagaa aagtatacca
    12361 gaattataaa aaataattaa aaaaaacagc gcgaaacaag gaacatatta cttcctcgtt
    12421 tcttgctgtt ttctactatt tcagcgctct tatgactcaa atctaaatgt ggttgtcaca
    12481 accttattct gcacgcaaag cgatagcggc gtcttttttc gctgccattc gtgcaggaag
    12541 atgaccgcca agcatcgtca atatagtaga gacgatcact agaataatcg catggacagg
    12601 atttagttga gcaacatttt ttagatccgt catgttgtat aagatgctat tgatagggaa
    12661 cgtagctagc catgcaataa acacacctaa tatcccagaa gaaataccta aaatacacgt
    12721 ttctgcgtca aatacgcgag tgatgtcttt tttacgtgca cctaaagctt ttagtacacc
    12781 gatttctttt gtccgttcaa tcacagatgt ataagtaatg atactgatca tgatcatact
    12841 tgttactaaa gagatccctg caaaagcaat aaggacataa gtgatagcat ccatcaatcc
    12901 gccagtcagt tcagtcatcg ttccagctaa atcagtataa atgattttat cttcatctga
    12961 tttgccttta ttgtaatcat ctagataatc caagatcttt tctttgtctt caaaattgtt
    13021 cggataaatc atgatacttg aaggcaagct gtctccacct aaataactaa gcaatgtttg
    13081 tttggcattt tcgtcaactt tttcagtagt taagacattt acaccactgt ctctttgagc
    13141 tttgacgatt tctgattctt tgttttcgtt gacgatttga gtcgttaatt gatcgctata
    13201 agcgattcct ggagataaga gattcattgt agaagaagat ttcacgcgta ggatccccga
    13261 gatcttcaat tcatcagaag cattctgata catcgcatca taatctgtat tagggataaa
    13321 attcccagtt ggaagttttg tatagtaagt attgttgttt actaatttga atgttgtacc
    13381 aacgatatca tcaaaatcaa gtttttcgtc ttctttcaca tcaaatccaa gattcttcaa
    13441 tgcgttgata ttagtattat tattgccgtc tacgatcagc acgacatctg ttgctgaggc
    13501 tggataactt ccggcaagta aagaataatt gtcttttaaa aagttttctt tgctagtgtc
    13561 aagctgtgta gggaaacttg acactcccac acctgtcatt gctgacatcg tactagataa
    13621 agaaagagat tccgtatcag gattttgatt cgagaagctg acaggctgta ctttgccatt
    13681 tacatcacgt aaaagattga ttcctgtagt acgggtaaat ccgatattat tacttaagtt
    13741 aggatcgatg tctgttacgt aatcgacgta tgtttgatca atcttattcg tgtgctgtgc
    13801 tttatcttct tcgctgactt ttgcagtaat cgttttgctg tctggataat ccgctttgct
    13861 tgcacccaat ccggcatcgt cacgagtctg gttagtcgtt accttagaaa tagtaatcgg
    13921 aaatttcgcc atcgtttcag cttgcgtgtt atcgatttgt ttttggaatc cagaagaaag
    13981 ggacagaacg atcgcgattc cgatgatccc aatactggaa gcaaaggagg tcaagaaagt
    14041 acgtcctttt tttgttcgga tattgttgaa ggaaagcttt aacgctgtcc aaaaactcat
    14101 ctttgttctg cgtaagttaa attgatcatc ttttggtcgt tcgatgtgag gatgggaatc
    14161 tgttaagatc ttaccatcag aaaattcaat gatccgatct gcgtattgat gggccagttc
    14221 aggattatgt gtgaccatga tcactaattt ttctttcgat aactcttgga tcaattccat
    14281 gatctggata cttgtttctg tatctaacgc tccggttggt tcatcgcaca aaaggatatc
    14341 tgggtcattg gctagtgctc gagcgatggc cactcgctgc atttgtccac cagataactg
    14401 atttggcttt ttatgcatat gatctgtcaa gccgactcgg tgcaatgcat cttctgcccg
    14461 ttttcgtttt tcgtctttag aaacaccact taatgtcatc ccaagttcaa cgttttcaat
    14521 aatgcctaga tgaccaatca agttgtaact ttgaaaaatg aaaccgatag agttgtttcg
    14581 gtaggcatcc cagtcactgt ccttaaagtc tttagtagac tttccgttga ttaccatatc
    14641 accggaatca taattatcta gtccaccaat cacattcagc atagtcgttt taccagaacc
    14701 actaggacct aaaatcgcta cgaattcttt ttgtctgaaa gcgacagaca caccatccaa
    14761 tgctttggtg gtggtttcgc cgactttata atattttttt atatctttta attgaagcat
    14821 aatcaccctt ctcccgtatt gaaatattcg gtatactaat aagtatatca gaacaatgat
    14881 cgtagtatac gaaataaatg ttaactgaag atgaactaca agaaaaaatc tagaaatttt
    14941 tcaaaaaaca gagggagatc aatgaatcgt attttagtag tagaagatga tccgcaatta
    15001 cagttgcttt accgttctgt gttggaacga gcgggctttt cagtcatcac cgcattgaat
    15061 ggagaggatg cgctgaaaaa actagaagcg acacgtatag aattaatcat tacagatatc
    15121 atgatgccga aaatggatgg ctatgacttg ctagactcat tacgtagcac cagatttgac
    15181 acacctgttc taatgattac ggcaaaggcc gattttgagg ataagaaacg cgggttcaaa
    15241 ttaggagcag atgattatat gacaaaaccc atcgatgtca atgagatgat cctacgtgtg
    15301 gaagcattgc ttcgacgttc aaagatcaat tacagcagta gcttgataat cggacaaaca
    15361 aaattagagc tagatgctta tacagtgtta caagatcagc attcgttgat tctgcctcaa
    15421 aaagaatttc tgcttctgta taaattactg tcttatccca acaagatctt cactcgccaa
    15481 cagttgatgg atgatatttg gggattagat acgaatacag aagaacgaac gatcgatgta
    15541 catatcaaac gtttaaggac ccgttttgaa aagacggaag attttcaaat catcacagta
    15601 cggggactag gctataaggc ggttatttaa agatgaaaaa aaagctattt tcacaattat
    15661 ggatctattt tgctagtatt gtcttcattt cgatcttagt gacactgctg tgttttctcg
    15721 gttttatttt cttattgtct cgacattcta tctcgcctgc tgaatcggct ggaaaaccaa
    15781 cgttataccc gttattcgtc tttgcggggt tcagtatggt cgtgggtaca ggaatatcga
    15841 tttttgttgg gcggcgtatc ctgcatccaa tcagtgcgct agggacaaat atgagtctgg
    15901 tagcaacggg agatttttcg attcgaatgg atgaacagca gaaagtagca gaagtacagc
    15961 aactgtataa agatttcaac gttatggtcc aagaactcaa tagtatcgag acactgagga
    16021 atgactttgt gtccagtgta tcccatgaat tcaaaacacc actagcaaca atccaaggat
    16081 atgttcaatt gttacaagcc ccaaatctct ctgacgaaga acgccaaata tttttatatc
    16141 ggattatcga aagtatcacg cagctttcac aattaacaga gaacatctta aaattgaaca
    16201 aactggaaaa ccagcgtatc caactggaaa agaaagagta tcgattagat gaacaaatca
    16261 gagaagtaat cgtttttttg cagcctaaat gggagaaaga acagctagaa ttggatatcg
    16321 aacttgctgc agtaaattac acaggcaatg aagagttttt gtaccaagtg tggctgaata
    16381 tcatggataa cgccataaag tacaatcaaa tcaatggaca gattcatatc aaactatttg
    16441 agacagccac agagattgga ctggaagtga ccgattcagg tgtaggaatg aatgaagaga
    16501 ccagagatcg aatgtttgaa aaattttatc aaggagatac aagtcgtcaa atttctggga
    16561 atgggctagg actttcactg gttaaaaaaa ttctagaact gcatgatgga cgtattgatt
    16621 atagcagtat cgaaggagta ggaacgacag caatgatacg gttaagtaaa caataggaaa
    16681 aagaataaaa cccctaaaaa ttctattgaa ggtttttagg ggtttttagt cgtttttttg
    16741 tgattaggga aatgttctta cggttctttt ggcataaaaa tcgctgcttt cttattgaac
    16801 tacgagaaat gttagaggtg tcattgaaac aaggacatga tgcaagaaag agagcgaaag
    16861 tcatgcgtcg ctctcttttt ttgggtggat agatggacag aaaatgtagg gcttagtttt
    16921 cttcttgatt taaatactcc caaaatgtat ctgaagccaa agaatgctca ccgactttac
    16981 gagtaaatgc aagataactc tctgttgttg gagaaatagg gagaatcaca gtattttctt
    17041 ttaatagatt cttgactgtt ttttccataa caatagcaat ccccatgtca tttgcaatca
    17101 tattgatgat caagtcgatt cttgctcctt tataagagat ttttggttca aatccagctt
    17161 cgcgacacag atctagtaca ggttgataca agttcgtagt ggggccaagg agcaaaaagt
    17221 tctccttttt cagttcgctt agatccaatt tctttttact agctaaagga tgatttttag
    17281 gtagaactgc tacaaaatcg tctgtttcag taggcaacca ctcaatcgaa cttggtgggg
    17341 cctggaaatt tctagcaaag tggatcgtgt cactatgttc attggcgtca ttgtaagtta
    17401 attcgacact ttccacttct ttcaactgga ctgtaaattc aggatggcgt ttcaaaaact
    17461 cggtgatttt ggaaaaacct ttataattga tcattgtcgg tatcgttaag atatttaatg
    17521 aaagattgtt gtctttcgta taggtttcaa gcacttcttg catagcatgg tattgttcta
    17581 cgacattttt tacatatggg agcaataagc ttccagcttc ggtcaattcg atttttctat
    17641 gggcgcgttc gaataatttg acgtctaatt ctgtttctaa cgaaagaatc tgtttagaaa
    17701 tatttccttg ggtggtgaat tgtcgatcag cagttttggt gaaattcatt gtttcggcta
    17761 aatcaagaaa aacttcgaat ttatggatat ccatcatatt cctcctactt attccaataa
    17821 agaatacttt aacacaaaaa atgaattggt gtaatcttct ctgtctgtta caatctttct
    17881 tgcagcgaaa gcaataatta cagaaatttt tctgattttt ttcgcttcgt agaaaaagag
    17941 aggagttttt tgatgccaac tggagttttg atcaatgtag gttccgtttt gcttggcgga
    18001 ctaattggcg gattagtagg aaataaatta agtgaacatt ttaaagcaca gctgacgatg
    18061 gtttttggtg tgtgttcgat ggggatggga atctattcca ttgcgccgat gaaaaatatg
    18121 ccggctgtta tttttgcttt agtgatagga acagcgattg gtctgattgt tcatttaggt
    18181 aatgggataa ataagggggc tgcgctgatg caagtaccta tttctaaaat cttccctagt
    18241 gaaaagcttg gtatgaccca cgatgaattt atcagcacat tagtgacggt cattgttcta
    18301 ttttgtgcaa gtggtacggg gatctatgga agtttagatt ctgggatgac aggagacagt
    18361 acgattttga tttctaaatc tgtccttgat tttttcacag cagctatttt tgcgtgtaat
    18421 ttagggtatg tcgtttctgt gatagcaatt ccccaattca ttatttttta tattttgttc
    18481 ttattggcaa aatttattta tccattgact acaccagaca tgatcttaga ttttaaagct
    18541 tgcggcggat tcttgatggt cgctaccggt tttagaatga tcaacgtgaa aatgttccca
    18601 gtagctgaca tgatacctgc aatgatcgtt atcatgccac tgagctggat gtggacaaat
    18661 tggattatgc cactgttata ataaaaatca aagagaagcc attttttgct gactaaaaag
    18721 gatcaaatct tttagtcagt ttttttgtat ttgacaaact gattttgaaa ctatattata
    18781 tgttgtataa tatagattga aataaaatat tacacttgag aatagaaggt gacgtaatgc
    18841 acatccagat tccaactgtt cttcttgatg gtactgtgct agcagcatta agcaaggaag
    18901 atatgtatgg gtatgcctta acaaaaatcg tacaagaaag tctctctgtc agtgaatcta
    18961 cactatatcc ggttttgaga agattgaaaa aagaaggata tcttgagacc tatgatgaac
    19021 cttttgaagg cagaaaccgt cgatattact cgttgacacc aatgggaagt cgtcatttag
    19081 aagaaattat tgatgaatgg attgtttttc gagattcttt agaccggtta ttggaggaga
    19141 gcacaaatga atgaatattt gaatgaagtt gtggatcatc ttcaagaaat tcctgaagac
    19201 gatcgttttg atttgatcca gtattatgag gaatattttt tagattctgg aaaaacatta
    19261 gatcagatca ttgatgaata tggtacacca aagcagtttg ccttaaaatt aaaaatcagt
    19321 tacttttcag aagaagaaca agcagaaaac gagttagtga aaaatccaaa aagacaaatt
    19381 cgcctgattt ggttaatcat tatcggttta tttgcttctc ctgtcctcat tccactagca
    19441 ctggggttta tattggttat tgggggacta ttggttgctt tactagcgct catttttagt
    19501 atctatgttt tatgtatcag tatcttaggt gttggcctat tcatgatcat ctcaggaatc
    19561 agtgtgtttg gacaatcgat tctgagtgga ttattcttta tcggtgtcgg tctgttcgcc
    19621 acaggaagcg cagtatttat ttcgccactg ttgctgaaag gaacaaaagg gcttttttat
    19681 gtcatgatga actttgttaa atggatggga cgacgtttta ttacaaaaag agatctgcat
    19741 tcagcgggcg tttgaaggag gacacataaa ttgagattga aacatttttt gattaccggt
    19801 attggtctga tgcttactgg tggcctccta gcaggtgcaa cttatacatt aggcgctcaa
    19861 aagagtctga cttgggataa tggtcctcgg ttgatcgata tggttactga gacaaaaaaa
    19921 atcaaggatg caaacaaaat cgtcatccat gcagaaaatc aaacagtttc ggtcatacgc
    19981 ggattggatt ttgagataaa aacgacttat gataagacag aaaagccaga aataaaggag
    20041 aaaaatggcg tgttgactgt gaatgcagtc aaaaaatcaa caggtgcaat cattagttta
    20101 gaagacacaa gtgcacaaat cactattgtt gtccctgcag atcatgaatt atctgaattg
    20161 acaatcgaag ggaataatag tgcaattgat atcaacgaag tatcaatcaa aaaagtgaat
    20221 actaagacac atgatggctg ggtatcgttc aatgaattta caggaggaac aataactgcg
    20281 aaagatgaat taggctcact tcgattagat gaggccaatg tggataactt gatcgtcaca
    20341 ggtgaaaaca gcaacttcca tctagaagac agtttgatca gcaaagaagg aaaagtcatc
    20401 ctaaacggtg gcaatttatc tttgcatgag atggaaaaca gtggctatac tatccaaacg
    20461 tctggtgaat cttatattga aaaagactac cagccaataa caaatacttt gttagaacaa
    20521 ggacagaaaa agctaaagat cactgctaaa gacacatcga tcaatattac gacggatcaa
    20581 gaggacaatg aaacagattg atcctgtact caactggata ttttaagaaa aaacagcaaa
    20641 gatccttcca atctttgctg tttttcatcg cttaacgcac ataactatag taaaataaga
    20701 aagaaaatat ctaaaaagaa aacagggtga aagagtatat gaaggtagaa gaaggtcaaa
    20761 gagaaaagtt aaaaacagaa atggaaagat tacatactta tatcactcaa ctaagccaaa
    20821 ccttctatga tccagacaaa gaaaaagtga tggttaatta cccgaacaat tcagaaggtc
    20881 gccaattaga gcaagtgtac catgaagttt tcaaacattt attgacggtg aagaaagagc
    20941 tggattatta ttctttaccg attatcgata caggaatatt gaagtatgat gggaagaagg
    21001 aacgatttat ctttaaatct gttcgggaag atttgcctct ttcagcaggg atggatctag
    21061 aggtcttggt agaagactat tttacagaag aaaagcactg ggttcgaaca aaattggatt
    21121 atttaccgca ggcagccgga gggacacagg caaggggttg gtacatcact gaagacaaag
    21181 agttagaact agaaggtgta atggcgcgta ttcgaaaaaa aattaaaaaa gtaacattag
    21241 atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag agcattttat
    21301 atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg attgtttttc
    21361 ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat ttttctttgg
    21421 ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca agcaaaagta
    21481 attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt ccttacgata
    21541 cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag ttctgtttta
    21601 aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt ctttaaagtc
    21661 gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt agaaatccaa
    21721 agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt caatgagaat
    21781 gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa agagctgcaa
    21841 gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca tttagcctat
    21901 gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat tttgtctgct
    21961 cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga gcaaatcgaa
    22021 gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa taatttactt
    22081 gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga catgaaagac
    22141 cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat tattgtcttg
    22201 aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat gatagttgat
    22261 tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga gagaagggga
    22321 aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac ttgcaggggt
    22381 gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa ttacgacaga
    22441 tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact atagatatat
    22501 cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg gacaattgac
    22561 tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata aaggtgtcac
    22621 ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa aactaattaa
    22681 agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa agtatacgga
    22741 aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt agaaaaaggc
    22801 gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt gaatatttta
    22861 tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga tgtgaccaca
    22921 ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat tttccaagat
    22981 ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct ttctttacaa
    23041 ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg tttatccatc
    23101 tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca acgggtcgcc
    23161 gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc tacaggtgcg
    23221 ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa tacaaaagat
    23281 catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg ccaacgtatt
    23341 ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca aagccgtgat
    23401 tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg ggagattgac
    23461 tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact acttggtgtt
    23521 attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa tggcaagcaa
    23581 cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt tccgattcgg
    23641 ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt cctttttgat
    23701 gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag gcagaaaaat
    23761 cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt tagtgggttc
    23821 agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc agttagatct
    23881 caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg ttttgttttt
    23941 tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca aaaaaccgat
    24001 tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata aattcttgtt
    24061 tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata tgatgaaaca
    24121 cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatca tattaggaac
    24181 ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga aatcagaaca
    24241 gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc gtattcgtga
    24301 atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg gcgctttgac
    24361 ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt ctggatatga
    24421 tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac tttcaccaac
    24481 actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata atgcaagtga
    24541 atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc cgaagatcac
    24601 gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg aacttcgaag
    24661 ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat cagaatttga
    24721 tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact tccaagcaaa
    24781 cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat ctttgcaaaa
    24841 agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc tattctctgg
    24901 ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg ctagctgttt
    24961 gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa tgcttgaaaa
    25021 gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag gcgtgttatt
    25081 cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa tgtttggtct
    25141 attcatgcaa aatccataca aagatatttg ggttccgttt acgatcttct tcgtattgta
    25201 tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga aaaagagatt
    25261 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag ctcctcatat
    25321 ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttgttcggaa
    25381 aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt attagaatat
    25441 agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt ccatgtatac
    25501 tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca atgaacaaaa
    25561 ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat ctttgtctgt
    25621 cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg aaaaacgaga
    25681 gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt atatcggtgc
    25741 ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag atgatgaaac
    25801 gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg cacctagctt
    25861 tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat cggtcatttt
    25921 ctatcagact agcccagata tgggaaacgg atttcctgga actttagatg tagaagtacg
    25981 gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca gtgaccgcaa
    26041 aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc cagcacagtc
    26101 aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca ataaagatac
    26161 aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc aacgtcctaa
    26221 aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg gcggcatcga
    26281 tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat tgacaagccc
    26341 agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga tttttacagc
    26401 aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc atggcggtat
    26461 cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg gagatgtcac
    26521 tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata taaagaaata
    26581 gaataaatta aaaagggagt gtgacagtgt cacatcccct ttttaattta ttctatttct
    26641 ttaaaggcag atcaagccag aatgatttgt ctaaacttaa caaggttagt acaggaacag
    26701 ttgcagcttt aactgtacga gtagttgttt ctggtatctg tcttgccgta aaattgttcc
    26761 acaaatccca gattgaagcg acacggaaga tggaagaagc gttgttgacg cgttttatcg
    26821 ttgaaaattc taatcctgtg attggcacgc catctgttgt ccatttgatt ggcacatcga
    26881 atcgcttaga catattgagg atttcagcgt ttgcttcttc agttgttgct gcttcttttt
    26941 tcggttcatc atattttgtc aggtcgtacg cttcgatgat cgcgttcaat ttttgtgaat
    27001 attggcgatc tgtagcataa cgtccttcca aatatttggt tgcttctttg tagtcttttg
    27061 tttccgactt ccaagttggt ttgtagaaat caggattgcc agtcagtcca tttttgatca
    27121 agtcggcata gtcttccaaa ctttccttgt aagaaggata tttacggaat tgtgcgcgga
    27181 tttggtagct ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg ctgcttcctt
    27241 tatatgatcc tttgatacca aaaaggttat gatttggttc gcttgataat aagctgtttc
    27301 cagatccact ttcaaggata gcttgagcca tcatgactga tgcgtagatt ccttcttttt
    27361 gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt gtttggtttt
    27421 gactgaaatg gaacgagcca tctaatccat ctaaaataga tgagccgcct gtcagcgaac
    27481 ctgaagcagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc gtgaaatctg
    27541 aaatagaagt tactcgaact ttatctcctg gttgaggcgc atggataaat tgtccgttcc
    27601 caatatagat acctacatgg tgagtaacac cggaagaagg tccccagaag acgagatcgc
    27661 caggttgggc ctgacttact ggaatctgcg tacctgcata ttgctgttcg cctgtccagc
    27721 caccgatata atgaccggtt gctttcatat atacgtaata agtcaaacca gaacagtcaa
    27781 aagtatttgg accttttgct ccccatacat atggttttcc aagatgcttg aatgcttctc
    27841 gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca attggtgatt
    27901 ttacaacaaa atcagaagaa gcagaagatg gattctctat accgctttga tttgctgtat
    27961 ttggacgaga tgaagcattg ttcgttccgc cattcgatga accattgttt ggatggtttg
    28021 gttctctctg agtatttcca ttgttattcg atccagatgg gtttgtttga ttcgaagaat
    28081 tcccgctgtt cgaattattg ttattattgt ttgaattatt tgaaccatta ttattgttgt
    28141 tattacccac atctggtttg tttggcgttt ccggatctgg tgtactaggg acttcttcac
    28201 taggatcagt tggtttctct gttgaagtat ccggtccagc tttaaattct gcagtaatag
    28261 ataaattgtt cgtacctact gtaaaggtct ttgcatcata atttacttct ttggcaccat
    28321 taacagcgac tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa gttaccttcg
    28381 tttccgctaa taaaccttct accgttccac tttccgtaat atccaaaggt tgctgttcag
    28441 gggaatccgc tgtcgaagca gtcagttgga tcttaccaat caaagaagga gcaattgtaa
    28501 gggaatatgt ctcggctggc ttttcatttt ctgatggatc agccggatca atttcagttg
    28561 gctcggtcga tcctgttgtt gagttagttg ttccagttgg gtcttctgta aaagaatccg
    28621 ttgtttcact agtagctgag ctggatgtag tcgtatctgt tgtgactgtt tgatcagcta
    28681 tttgattatc tacggcaaat acggtattgg tcgacaaaac agacggagca atcagccaag
    28741 tagcagctag caaacgtatc gtttttttat ttttcataat aaactagtcc ttttcctttc
    28801 attgattttc tgtcttattc agcattcatt agacattttt tggaacagat aatcgtgtcg
    28861 aaaagctttt tcttttcata aatctgtctg tgtattctaa ataaaacaat ttgtaaataa
    28921 gcaacgagca ttcattcgtt tttctttgac gttcatttta ttgcatagac cacctttgtt
    28981 tggggataaa caagtgggga taaaaatggt tatgcatatg taaattatac caaagtttgg
    29041 aaagtatcac aaaaatttat aaaaataatg atcctaaaaa gaaaaagact gaaatatgtg
    29101 ataaacatct catacttcag tctttttcat taactattca taagacatta ggtcttcatc
    29161 aaacccgcta acgagagtag tgatcattgc tacgactaga ctaacagcca gtcctgctaa
    29221 gtaccagaaa aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac caaatacaaa
    29281 agcgatcgct ttcacttggg caaatcccat tacagctcca ccagcagctg caccaatgca
    29341 agcagcaaca aatggttttt tcaatgggat cgcaattccg taaacagttg gttcggtcac
    29401 tcccgcaagt aaaatcgaag caaaagcaga accggagatc gaacgggttt ttgaacgttt
    29461 acttcgcaga aatacaccta aagctgcacc agccattcca aaattggctg ctcctgtcaa
    29521 cggaacgatt cggtcgaacc cataagcaga gatattactg atcatgacag ggtttacagc
    29581 ccattggata ccaaatatta ccaaaatatt ccaaccacca gcgatgattg cgccactgat
    29641 cggtccgtta atatccatca tccaagtaat tgcggcagct aaaccttcgc caaggtaaac
    29701 acccgcaggc ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg aaagtaaagg
    29761 tacaaatact aattgcatat tttcgcgaat atattttttg aggaattttt caacaaaaga
    29821 ataaaagaaa atacctaaaa tcgccggcat gacagtagac gtatactgca tcagtactac
    29881 aggaatcccc agaaaatcag tagctgcttt gccactgtct gaaagtaaag aggtcagatt
    29941 cggttcaatc agtgctgcag caatgacagc cgcaatatat ccattcactt taaacgtttt
    30001 ggcagcagaa aaagcaagaa agattggcag aaaataaaag acggcattcg acgcagcaga
    30061 caaaatatga taagttccgc ttgtttcact aagccagcca atcgtagtcg ctaaaacaac
    30121 cagacctttt aatataccag aaccggcaaa cagcggaatg atcggcgtaa agatttccga
    30181 tatcaaacca aatagttttt cgatcggatt tagcttttct ttcggagctg aatgatcgat
    30241 ttgacgcgtc aattccttgt aagcaggagc caatccctga tccatgatta tttctgcata
    30301 tctatcgagt aaaatgaaat acttaatgga agggatttgc tgttttaatt tttctgcatt
    30361 ggctactggc tgttttttca atttgatacg aagccgagtc gcacatggaa ggacagaatc
    30421 aatattgtct tttcctccca actgttcaat cagcatatga atctgctcgg acagtaccat
    30481 ctgttcagga ttcattgtcg tttccattag aagaccactc cactcactaa tataatattg
    30541 gcgtaaggtg tcatttcgcc tgtacgaata aatgcatgac agttattcaa ctcttttttc
    30601 atttcttcat gtgggataaa gctaatcggt gtatctggta acaataattt gattttttcc
    30661 aatatttccg ggttcattgt ctttatttct tccgccaatt caatcgattg tacctcaagt
    30721 tcttctaaaa cgttttctaa aacagataaa aaacttggaa gaccgtttga gacagccaaa
    30781 tctatttttt cagtaccaaa ggggacaggc atgcctgcat ccccgatact taatttatca
    30841 aagtgcccca tttgtgcgat tactcgtgaa atatctgaat tgattacttt tccttttttc
    30901 atgtttgttt cttcctctca ttgttaattt tagactgctg attgttctaa agcttcgcgg
    30961 taagggatag aaggttgcgc accataagac tgcacagcaa cagaagaggc tagatttcca
    31021 taaaggatcg cttctttcaa gttactaagg tctggctgaa gtgtgctgct taatgcgcca
    31081 ataaatgtat cacctgcggc tgttgtgtca acggcatcca ctttaaaagc agggacgata
    31141 ccgtgttctt tttctgtatg atagaaagcg ccggcactgc ctagtgtaat gattactgtt
    31201 ccaataccta attcatggag tttttcggct gctgcgacca aactgttatg atctgtcact
    31261 ctgattcctg taatgatttc tgtttctgtt tcatttggta taatgatatc tgtcttttca
    31321 agtagttcag taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt tgtttttcct
    31381 gcgtcttttg caatagaaaa agctaggatc gtcgcatcta agctactttc aaactgagca
    31441 ataataaagt cactgtttgt aatcagttgc ttgtttttta ggacttcttg ttcatggaaa
    31501 gcattatttg cccctgcatg gatcaagata ctattttctc ctgctgcgtc taccataatc
    31561 attgcttgtc cggttgtggc gccttggatt tcagctatcc cagatgtatc gatgttttct
    31621 tttgtcagta gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc tccaataaaa
    31681 gatgtccgtg cgcctgaacg tttggctgcc actgcttggt tggcaccttt acctccgcca
    31741 gcgtgaaaaa tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt ggttaaacga
    31801 agggtagtgt ccatattaat acttccaatt actgtaaccg tgttcatttt acaaacctcc
    31861 aactatctca tttattttga tgggaaaatt attttttaaa acgttttagt aaaacgtttt
    31921 aatagttcga taaaaatata gcacaccttg aaagctattt caagggtgca gttgaaaatc
    31981 ttttttctaa tcgtacaggt aatctttttt cttcccattc tttttctggg aattggatcc
    32041 gatctaacaa taacttggca gatgtttgtc ccaattcgaa gattggttgt gcgatcgttg
    32101 tcagtttcgg cttgatatac tcgcacatat ctatattatc atagccaata atgctgtagt
    32161 cttcagggat gctttttcca gcttcctcta atccgcgata taatccaaaa gctaattcat
    32221 catttaaagc aaacacacca gttgcagcag aatcaagaag ttctgctgtg atctggtagc
    32281 caccttgttt tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg atttggtaaa
    32341 catcaagagc agatttaaac ccctcaatcc gagcgtggac attttccggt ggattttcag
    32401 gatagacaag ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc gctaggtaac
    32461 caccgcgaaa atcgtctgtt cgtacggcat cactaaatcc ttcagatttt ttctgatcca
    32521 aaacaatgaa gggccggcct tgttttttca aattttcgtt gattgcatct gtagagacag
    32581 cagaagtcgc gataatgaat ccatccacac ctcgacgtgt gagctcggct agatattcga
    32641 tttctttttg atgatcagaa tcagcattac ataatatcgt gacgaaattc tgcttataca
    32701 agatatcctc aattcctcgc atcagtgtac tgaagaatgg attcgtgata tcaggaacga
    32761 gtacaccaat tgttttcgtt tccttggtaa tcatttgtct agcaaaatag tcaggctgat
    32821 agcctaattc ttctttggca gctaaaactt tctgaacagt tttcggactg aatttctccg
    32881 tatttccatt taatatcaag gagacagttg tgattgaaac gccggaaagt ttagcgactt
    32941 ctttgattgt gatctttttt ttcaccatgt tcatactcct atctaaatcc tgtggttatt
    33001 ttcccatgaa atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt atttgctttg
    33061 ttggatagct tcgattattt gtttcgttga gcgtactcgt gcagttttcg gaaatatctt
    33121 ttttatgaac aattgatggg attctttgtc acgatcactg caagcatctt cagcaagcac
    33181 gatatggtac ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt ttgttgtcgc
    33241 tacaccgctt aaaatgatag tttcgattcc acgccggcga agttgaagat caagagaagt
    33301 accaaaaaaa gcacttggat tgtatttttt gatagtcaac atattatctg tatctttcaa
    33361 agaatccttc aaaagtaaat cagtgaattc tggaggtaca gaagcagtca ttccacccat
    33421 atcagtttgt tgactcaaat aagggaaagt ggagatgtct acgttcacta aaactttcaa
    33481 ggcagcggta cgtttcaatg ttcttactaa caaatcattg tttgtcagaa tagatgctgc
    33541 agtgtgtgga gagagtgtac cgttatttac gatctctttt tgcatgtcga tcgcgacaaa
    33601 agcagttttt gataaatcaa aaatcatatt ttcctttaaa tcattcatga tgttccttct
    33661 ttcaaataac aatgtttttt atgtaagtaa atcataaatc gtttatttgt caactaaatt
    33721 aaaggattcc tttctttagt ctagagaaat tcagtggtaa aataaataag tgtgagaaaa
    33781 cgcattcgat gcatagatat agcgagaaat atcgtgaaaa tggaggaatc agagtgaagt
    33841 atgtcgaagt aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc attacagcaa
    33901 atgacgatat ttctttttca attgaaaaag gcgaattagt gattatactt ggtccaagtg
    33961 gagcaggaaa gtcaacgatt cttaatattt taggtgggat ggatacacct gatgaaggtc
    34021 agatcattat tgatgacaca gatattgcgc aattttcaga taaacagctt actgcttacc
    34081 gacgaacaga tgtagggttc gttttccaat tctacaatct cgttcctaat ctaacagcaa
    34141 aagaaaatgt ggaattggca acggaagttt ctcctgatgc gcttgatcct gtagaggtat
    34201 tgaggcaagt gggcctagct catcgattga ataactttcc gtcccaatta tcaggaggcg
    34261 aacagcagag ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt cttttatgtg
    34321 atgaaccgac aggcgcgctg gattttgaaa cgggcaagca agtattgaaa ttattgcaaa
    34381 acgctagcag acaacacggc aataccgttt tgatcattac acataattct gctttagcgc
    34441 ccatcgctga tagagtcatt cacattaatg atgctaaagt aaggtctgtt gaattaaatg
    34501 atcatccttc atccattgat gagattgtat ggtaggggga ttttatgaaa acaagaacat
    34561 atcttaaagc aggttttcgc gaaattcggc aatctaaagg acgattcatt gcaatcatcc
    34621 ttattgtcat gctaggaaca cttttatttg taggtgtcaa aactgctgga cctgtcatgc
    34681 aaaaaacgat ggatcactat gttggtacag caggactttc tgatttgcaa atcgtttcta
    34741 ctggtggctt gacacaaaaa gatattgctc aagcagaaaa gatcccagat gcaaaagtag
    34801 aaacaggaaa acagatctat tattccaata ctggcaaaaa cgaagtgatt caaatatttt
    34861 cttataacaa aaataacaag cagaacaagc tacaattaac tgatggaaaa ctcccggaaa
    34921 aaccaaacca gcttgtattg gatgaaaaag cggaagatga agggtataaa atcggtgata
    34981 tttatcagat tgattcagat gaattgaaag aaaaagagta taccatcacg ggctttgtcc
    35041 gttctccatt gttcatcaac aatttggaga gaggatacgc gaacgtcgga aatggtagcg
    35101 tcgattattt tgtctattta cctgaatccg tttttaaaac agatatttat tctgtcttat
    35161 atcttgattt tacaaacgta aagaatttga atacgtacag caaagcttac aaagataaaa
    35221 tggagcaaaa tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa gaacgcttag
    35281 aagagctgaa aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa atagcggatg
    35341 gcaaagcaca aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa cagttagcgc
    35401 aacagtcagc aactatccag cagcttcctg aagcgcaacg aacaatcgct gttcaaacat
    35461 tacctcaaca agaagctcaa cttgctgaac aagaaaggca attagttgag aaagaacgtg
    35521 aactggcctc tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa aatttaacta
    35581 aaccatcata cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa tttggtgatc
    35641 tgtctgatcg tatcgctgcg attgccaatg tttttcctgt tttctttttc tttattgccg
    35701 cattgatcac ctttacgaca atgacacgta tggtagaaga aaaccgcagg gaaatcggga
    35761 cattaaaggc gttaggttat acgaaagttg aaatcgcaaa aaaatatgca atctatgcaa
    35821 gtctagcctc agggataggt attgttttag gaacgatatt aggaacgaat cttttgccaa
    35881 ggatcatttt tgagctgtct aacgagcggt atgatattgg gagtgcgctt attttttatg
    35941 actggccgcc gattttacaa gcagctgccg cattcttcat tgccgcattt ggcgctgcaa
    36001 tgatcgtttt attcaaagat ttaagagaac ggcctgctgc attgcttcaa ccgaaagcac
    36061 cgaagccagg gaaacgcatt tttcttgaat acatcactcc gctgtggtca cgccttagct
    36121 ttaatcaaaa aatcagttac cgaaatctct tccgatataa gtcaagaatg cttatggcga
    36181 ttatcggaat tgctggatgt gccgggctga tggttgcagg cgtgggattg aaagattctc
    36241 taagttctgt ttcagccaaa caatttggac cgatcattga ctatcaagca atcgttacat
    36301 tggattcatc cacggaagat agtcgtcaaa aagcagcgga tatgctgcag aagcaagaaa
    36361 aagtaaatga gatcctagct attcatactc aaaccgtaga attgagaaaa aaaggacatg
    36421 cttcgcaaag tctgacagtg atcgtaccaa gtaaaacagc tgatttggat cgttttatcc
    36481 atttaactga tcttgaagag aacccacgct ccttgcctaa aacaggaatc gctattacgc
    36541 aaaaagtagc agaactattt gacttatcta caggagatca tatttcgctt tatgatgaag
    36601 atcaaaaaga atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta ggtagcttta
    36661 tttatatgtc ccctgaatat tatgcttcag ttactgatca atcgatgatg gataatgcat
    36721 ttttgctgcg tacagataag atgacaacaa aagaagaaga aatgctttcc gagaaactgc
    36781 ttgccacaga cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa acgcaagaag
    36841 aatcaatggc gaacttagac tcggttgtct taatctttgt cgtattatca ggactgctgg
    36901 cttttatcgt cttgtataat ctaactaata tcaatatttc cgagcgtgta cgcgaattat
    36961 ctacaataaa agtccttgga ttttttgata aagaagtcac gatgtacatt gttcgagaaa
    37021 atatcatctt tactctttta ggaatcatcg gaggatttgg tgtaggatat atattgactg
    37081 acttcatctt gcaacaagcg tctatggaga atgtcatctt tccattagtg atcacatggt
    37141 gggcttatgt cttatcagca ggattaacta tcgtatttac ggttattgtg atgattgtta
    37201 cccatttcaa attaaagcat atcgatatga ttgatgcgtt gaaatcgaat gaataaaaac
    37261 agaggttgtg aaatcagcgt tttgagctaa taacgagggt cagctgctaa ttgaacacca
    37321 tgaatcagag gttgtaaaaa agccgtcctg catcgagcaa aaagaatact caaataaaat
    37381 tagctcctca tgttttacat ttgagaagac tgattgccgt agataaaagc ttttgaacac
    37441 catttataga agaaacaaga atattttgct attttaaaca agaggataga caaatacatg
    37501 tctgatctct tgttttttat tgggcaacag acctcttaga atgtataaag ataaatttta
    37561 caaacgcttt ctgttcagtt ataataataa atgataactt gtatatataa gctgtggtag
    37621 gtaataaatc agttgaatgg aggtaaatat ccatgacatt taaagacaaa gtcatttacc
    37681 aaatctatcc caaatcttac aaagacagta atggtgatgg aattggagat ttaaaaggaa
    37741 tcaaagaaaa actcccgtat ctgaatgatc taggcatcga catgatttgg ctaaacccta
    37801 tttatccaag ccctcaaaaa gacaacggtt atgatatctc tgattacaca gccatcgatc
    37861 ctatctttgg tacgatggag gaattcgaac agctaatagc cgaagcaaag aaataccgga
    37921 tagaaatcat gctcgatatg gtgctgaatc acgtttcgac agaacatgaa tggttcaaaa
    37981 aagcactagc aggggaccag cattatcagg acttcttcat tttaagagaa gagccaacgg
    38041 attgggtatc aaaatttggt ggaagtgctt ggtcgccatt tggagatacg gggaaatatt
    38101 atttgcactt atatgatcgg acacaagctg acttgaactg gcggaaccaa gcagtaagag
    38161 aggaactatt caaagttgtt cgtttttgga tggaaaaagg ggtaaaagga ttccgtttcg
    38221 atgttatcaa tgttatcggt aaagacgaac aattgaaaaa taataaagaa aatgatggca
    38281 aacctgaata tacggacaaa ccaatcaccc atacgtatct aaaagaattg aaccaagcaa
    38341 cgtttgggac ggattccact atcatcacag tcggtgaaat gagctctacg acgatcgaaa
    38401 attgtatctt gtatacagaa cctagtagag aagaattatc aatggtcttt aatttccatc
    38461 atttgaaagt cgattatgaa aatgggaata aatggtctac tcctccattt gattttgcag
    38521 aattaaagcg actatttcat acatggggag aaaatatgag tgagggtaac ggctggaatg
    38581 ctctattttt taataatcat gatcagccac gtgcgctcaa tcgatttgta gttatcgatc
    38641 agtatcgaaa acaaggagca gagatgctag caaccatgat ccatttgaac cgtgggacgc
    38701 cctatattta tatgggagaa gaaatcggca tgatcgaccc agactattca tcgatttccg
    38761 actatattga cgtagaaagt ttgaatgctt ataagttact ccttgaacaa ggattcacag
    38821 aaaaagaagc gtttcgtcgg atcaaagcaa aatctcgcga taactctcga acacccatgc
    38881 aatggtcaga cacagaacaa gcaggattta ctacagggaa accttggtta aaagttggag
    38941 gcaagctaga agagattaat gtagaaaaag aactatcttc tgaagattct attttgtcct
    39001 attacaagaa attgattcgg ctgagaaaaa catatccaat cgttgcacaa ggagattatc
    39061 atgcttatga agcaaatcat ccggaagttt atggctttct tcgccaattt gaaggacagc
    39121 agctgcttgt attaacaaat ttctatgata aagatacaag gatcgccatc ccagaaggat
    39181 tcacagaagc caatattctt atcgataatt attccgatac tgttcttcat caaagtctgg
    39241 agctaaaacc ataccaggca attgctttgc tgaaaaatta aaacaaaaac acaaaacgtc
    39301 gttcctacga gctattctcg taagatcgac gttttgtgtt ttatttaaaa taatcttctt
    39361 cagaaaatga agagttttca tactctcctt tatcgccata aaatttattg tagcgttgtt
    39421 tgaatttacg gaaaataaaa gtaacgagta atttgactaa cgtatagata ggaataccaa
    39481 aaataacgcc cacaattccc attagatcac ccatgacaag taaaacgatt aagatcgtaa
    39541 tcggatggat ctgtaaacga tcacccatta ctcgaggaat caccaagtca ccgtggagaa
    39601 gttgtacggc aaaccagaca atgacaaatt tgacagccat aaaagtagag tcttggaatg
    39661 caatgatcaa acctggaaag aaggcgataa acggcccaat atatggaatg atacataaca
    39721 acccagcaga aatagctaaa acactagcaa attctaatcc gatcagtaag aaacaaaccc
    39781 agtagactgc tccaaggata aatgaagcaa tgatttgccc ctttagaaat gaacccaatt
    39841 gttgattagc aatctttgtc aaatttttaa aatctgtacg aaatgctggt gggacaatcg
    39901 ctaaaactga ctgataaaat ttttgaggat ctctcaaaag gaaaaaagcg acaattggac
    39961 cagtaaataa agtaataaaa atagctgtaa cagttgaaaa aatgttgctg atccgttgtg
    40021 cgccactttc ccaataattt ccaataaatc cagctatatc agacgtcacg tcgtcaatag
    40081 aagcaaaaaa ttgattaaat gaatcggcaa aaggtgtttt ctccaaaaat tgtcggagac
    40141 tctcttgaaa atcttgaaac aaatctggca aactactgat caatgtcttt gcctgttctt
    40201 caagaaaagg aataagccat atgcttccaa gccccaataa aaataaaata atcacataaa
    40261 cgataatgac tgacaagttc ctcgaaaggt gcttgctcaa acgtttgatg actggttcca
    40321 aaagataata caagatcagt gcaaacaata atgggggcaa gatagtagcg ataatgattc
    40381 cgacaggctt gaatataaac gagatctgat ggaaaaaata aatgatcagc gcaagtaaga
    40441 taactattcc taaaacatag taagaggttt ttcctcctaa aaagcggatc aaacgattcg
    40501 ttgtatctgt ttcttttttc actctttttc ctcctttatc tataacttca ttatagacaa
    40561 aagcgggtat aaaaggaaaa tgatagaggt tgattgcaaa aatatcgtca atcattcata
    40621 taaactttct catcaattaa aataagaaaa cgcttgacag ttcttttaac catatgataa
    40681 attaaatcct gttgctaaaa cgtaactatt acggtttgcg aaaagcgaat cggaacattc
    40741 aaatgaaaga agatgagaag aggagtgtca taaatgaaac gattgatagg tatattggcc
    40801 atgctggttt tagcaggtat gttaactgct tgcggggcaa gtgggaaagc cgaggacagt
    40861 aaagaaaaat tgagcgtgat gacgaccttt tatcccatgt atgattttac aaaagcaatc
    40921 gtcggggatg aaggagaagt agagttattg attccagcag ggactgattc ccacgattat
    40981 gaaccttctg ctaaagacat ggcaaagatc caagatacag atatctttgt ttataatgat
    41041 gaaaacatgg aaacatgggt ccccgcaatc caaaaaacat tacaagaagg caatgtccat
    41101 acgataaaag cgacagaagg tatgctttta cttccgggaa gtgaagaagg acatgatcat
    41161 gaccatgaac acggggaaga aggccataca catgagctag acccacatgt atggctagct
    41221 ccaagtttag caatcaagca ggtagcaaac atacgtgatc agctgattga agcttaccca
    41281 gaaaaacagg aagtatggac aaaaaatgca gcagcctata ccgagaaact tcaagcactc
    41341 catcagcttt atcaagaaac ctttaagcaa gcaaaacaaa gatcttttgt cacgcaacat
    41401 gctgcattca actatctagc gttagaatat ggattgaatc aagtatcgat tgctggtctt
    41461 tcttctagtg aagaaccgtc tgcggcgaga attgctgaac tgaaacactt tgttaaagaa
    41521 catggaatca actatattta ttttgaagaa aacgccaaag atagtattgc cagaacgtta
    41581 gcaaacgaag caggtgtatc tttagaagta ttgaatcctt tggaaggatt gacgaatgaa
    41641 caaatagaaa atggagaaaa ctatctttct atcatggaag caaacctcga agcgttgaaa
    41701 aaaacgacag agacagaaaa cccgctagaa gatactttga cccccaaaaa agaaaagagc
    41761 gtctacaacg gttattttga agacgaggat gtacaagatc gctctctttc agattgggca
    41821 ggaacttggc aaacagtaga tacgtatgta gaagatggga cttttgatcc tgtttttgaa
    41881 tataaagaaa aattgaatca ggataagaca gcacaagaat ataaagaata ttatacgaag
    41941 ggctatcaaa cagatattgc ttcaatcaag atcagtgaga aagagatggc atttacgttt
    42001 aaggatggaa caacgaaacg ttcaaattat cgttatgtag ggaaagaaat cttgacctac
    42061 gaggcaggaa accgtggcgt aaggtatcta ttcgaagcag aagataagga aagtggcgct
    42121 tttagatata ttcaattcag tgatcacacg attgcaccgg ctgactccga acattttcat
    42181 ttatatgcag gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca
    42241 ttttatccag atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag
    42301 gcaaaaaa
//
LOCUS       DAZULY010000003        35487 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000003 DAZULY010000000
VERSION     DAZULY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.003"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1424)
                     /locus_tag="ACG81A_000078"
     CDS             complement(<1..1424)
                     /locus_tag="ACG81A_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5013489.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRL"
     gene            complement(1414..2709)
                     /locus_tag="ACG81A_000079"
     CDS             complement(1414..2709)
                     /locus_tag="ACG81A_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5013490.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(3041..3718)
                     /locus_tag="ACG81A_000080"
     CDS             complement(3041..3718)
                     /locus_tag="ACG81A_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFS5013491.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(3748..4839)
                     /locus_tag="ACG81A_000081"
     CDS             complement(3748..4839)
                     /locus_tag="ACG81A_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFS5013492.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(4940..5374)
                     /locus_tag="ACG81A_000082"
     CDS             complement(4940..5374)
                     /locus_tag="ACG81A_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS5013493.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(5958..7415)
                     /locus_tag="ACG81A_000083"
     CDS             complement(5958..7415)
                     /locus_tag="ACG81A_000083"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5013494.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(7412..9307)
                     /locus_tag="ACG81A_000084"
     CDS             complement(7412..9307)
                     /locus_tag="ACG81A_000084"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5013495.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(9365..11140)
                     /locus_tag="ACG81A_000085"
     CDS             complement(9365..11140)
                     /locus_tag="ACG81A_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS5013496.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(11190..12686)
                     /locus_tag="ACG81A_000086"
     CDS             complement(11190..12686)
                     /locus_tag="ACG81A_000086"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5013497.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(12880..14502)
                     /locus_tag="ACG81A_000087"
     CDS             complement(12880..14502)
                     /locus_tag="ACG81A_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS5013498.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(14951..16264)
                     /locus_tag="ACG81A_000088"
     CDS             complement(14951..16264)
                     /locus_tag="ACG81A_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS5013499.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(16509..16733)
                     /locus_tag="ACG81A_000089"
     CDS             complement(16509..16733)
                     /locus_tag="ACG81A_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013500.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(16753..17175)
                     /locus_tag="ACG81A_000090"
     CDS             complement(16753..17175)
                     /locus_tag="ACG81A_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5013501.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(17319..18029)
                     /locus_tag="ACG81A_000091"
     CDS             complement(17319..18029)
                     /locus_tag="ACG81A_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFS5013502.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(18047..18544)
                     /locus_tag="ACG81A_000092"
     CDS             complement(18047..18544)
                     /locus_tag="ACG81A_000092"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5013503.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(18581..19033)
                     /locus_tag="ACG81A_000093"
     CDS             complement(18581..19033)
                     /locus_tag="ACG81A_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5013504.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(19168..19773)
                     /locus_tag="ACG81A_000094"
     CDS             complement(19168..19773)
                     /locus_tag="ACG81A_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS5013505.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(20223..20843)
                     /locus_tag="ACG81A_000095"
     CDS             complement(20223..20843)
                     /locus_tag="ACG81A_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5013506.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(20845..21714)
                     /locus_tag="ACG81A_000096"
     CDS             complement(20845..21714)
                     /locus_tag="ACG81A_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS5013507.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(21737..22144)
                     /locus_tag="ACG81A_000097"
     CDS             complement(21737..22144)
                     /locus_tag="ACG81A_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS5013508.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(22163..23452)
                     /locus_tag="ACG81A_000098"
     CDS             complement(22163..23452)
                     /locus_tag="ACG81A_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5013509.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(23479..23781)
                     /locus_tag="ACG81A_000099"
     CDS             complement(23479..23781)
                     /locus_tag="ACG81A_000099"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5013510.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(23806..25899)
                     /locus_tag="ACG81A_000100"
     CDS             complement(23806..25899)
                     /locus_tag="ACG81A_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5013511.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(26388..27152)
                     /locus_tag="ACG81A_000101"
     CDS             complement(26388..27152)
                     /locus_tag="ACG81A_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFS5013512.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(27263..28132)
                     /gene="mdcB"
                     /locus_tag="ACG81A_000102"
     CDS             complement(27263..28132)
                     /gene="mdcB"
                     /locus_tag="ACG81A_000102"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFS5013513.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(28157..29233)
                     /locus_tag="ACG81A_000103"
     CDS             complement(28157..29233)
                     /locus_tag="ACG81A_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS5013514.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(29257..29850)
                     /locus_tag="ACG81A_000104"
     CDS             complement(29257..29850)
                     /locus_tag="ACG81A_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFS5013515.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(29854..31530)
                     /gene="mdcD"
                     /locus_tag="ACG81A_000105"
     CDS             complement(29854..31530)
                     /gene="mdcD"
                     /locus_tag="ACG81A_000105"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFS5013516.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(31520..31819)
                     /locus_tag="ACG81A_000106"
     CDS             complement(31520..31819)
                     /locus_tag="ACG81A_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFS5013517.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(31921..33567)
                     /gene="mdcA"
                     /locus_tag="ACG81A_000107"
     CDS             complement(31921..33567)
                     /gene="mdcA"
                     /locus_tag="ACG81A_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFS5013518.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(33574..34485)
                     /locus_tag="ACG81A_000108"
     CDS             complement(33574..34485)
                     /locus_tag="ACG81A_000108"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS5013519.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            34605..35462
                     /locus_tag="ACG81A_000109"
     CDS             34605..35462
                     /locus_tag="ACG81A_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5013520.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
BASE COUNT        10147 a         7566 c         5964 g        11810 t
ORIGIN      
        1 ttcaatcgaa taatgtcgta aggtgacgca aattctgaca gataatattc acactgtttt
       61 ttgtttctct ggcaaaatgt gatcaccaaa tcatattctt taccttcttc ataacttcca
      121 atctcaatac cgccgattcc cctcagttct ctgatcagat attgttgaaa agcaatacga
      181 taaatcggca gactttctaa atcgattcca atcactactg gttttgcaat ataaaaatcg
      241 atcatcaaaa gaacattggc atactggatc atcaggtaac taagctcact atcttcagct
      301 cttttcctcg gcaattgctg caccgctgtt tcctgtaatt tcgccagtaa ctgtgttaag
      361 gaatgcccga gcattttctg ctgctgttcc aaaagccgat cacgctcata gacttcgatc
      421 tttccaacaa agaaaaacca ttcattgttg atctgggaaa tttggtagcc tactgccttt
      481 tcttctttga ttttcaaacg acgaggacga taatgccata acatcgtttc ccgaatgtaa
      541 gtatctaaaa cagcggttgg taactttttg ctccgtgtca aatcatactg ataaaatatt
      601 tcttcgtcta ctatcgaaaa agaaacaaaa aagctgtaaa acatcattgt ttcgtataca
      661 ttcagttctg ctgctgttcg actaagatgc atcacgatga ttggatcgat tgcttgataa
      721 agccggtccg actgatagag taatttcttt tctttcaaag ttgcgtacgt acttttttca
      781 ttcaatagtc tttttttcgt aatcccgagc cagcaagcaa tctgtgcttc tgctgatgcc
      841 gtaaacgttg tttctaatac acggtccaat cctagaatga acggtctttt ttctggtgta
      901 ttttgcaaaa aaagcggtcg ctgatcttcc gggatatatt ggaatagttc ataataaaaa
      961 tatcgtatct gtagctcttc tccgtataat tgaccgtttt tgatttgtaa tccaaattcc
     1021 tctaagagct ggttcaattc tttgatcttt cgaaaaacag aagactcact gatattaaac
     1081 gctgtcgcta atcgaacgat ggatacttgc tgatgttcta ataaatagac gagcatctta
     1141 aacttcaagc tttcctgcaa taaaaacaat agaatttttt ccaaacttac atcttcgtcc
     1201 aacacaagca gcgcctgata ctcatttcgc tgtacttcta cttttttttc catcattcgg
     1261 ccaatcaatt caatatcttc caaatattga tcaaacgagg cttttgacag ttggacatgc
     1321 tctctcaaac cattgataga ggctgttccc ccttgcagaa caaggtttcg caaaatcagg
     1381 atctgtaact gctcttttct ttctaataat gcttcaatct tcataggcag tattcgccac
     1441 ctttgaccac accatatctg gcacacggta ttctggctgg aaaaatagct catgttcttc
     1501 taactgttca aacgtccatc cttcttctac tgcaattgtc agttggtcaa gaaggtggaa
     1561 catctctttt ttgctttgga gctgtgctcc aagaatcttt ttttcagaat caaatagaaa
     1621 tttgagttta actttgcttt gatttattgc tgtaagcttg tagtctacgt atcctgtttc
     1681 aattttttgg ggataaaaaa tgctctcttc ttctgtcaaa cctacgcttc ctaagaaata
     1741 gccaaaccac tgattgccga ctggacgata ggttccctga tcctttgtct gtgagccact
     1801 aattgtcttg ctgacatttc gtgctgtacg gacagcgtta tttactagag aaacatacat
     1861 tttttcattt gttggacgaa aagttacttg gatcgcatcg ccaatcgcat aaacgtcttt
     1921 ttgagacgtt tgcaagtagt catctaccca gatcgttcca tcatcattca atgttaattg
     1981 ttcttcccac atctgattgt cggggcgaga atgattagcc agcaaaacaa gatcatagtt
     2041 ctcctttctt ttttccgtct ctacaatagc ggtttcattt tcggtcaaag acacaacttg
     2101 ttcttctaaa aaaagtgaaa gcccttgatt tttcatttcc tttactaacg gttctgtcat
     2161 ttcaggatct agataacgaa aaagaattga ctttcggctt tcgtaaagat ggatttgttt
     2221 ttttcttgat gccaaccctt cagctatttc gattcctgct tgtcctgcac caataattgc
     2281 aattttcttt gcattcatga tcttttgctg aagagccgtt acgttttgta tctttttaac
     2341 tgggtgaata agcgttgatt ctgcagaaat atttcgaaac ttttgatttg aaccagtcgc
     2401 tagaatcaaa cgatcaaaat ccaatttttt tcctcctgct aaaacgactt ttttttcttt
     2461 caatcctatt actgtctttt ctgtgtaaac atggattcga tagcggtcag tcagttcttc
     2521 tttcgtgatc caagaaagtt gtttttcact ggtaaccttt ccttttaata tcaaattgaa
     2581 cccgctggga atccatccta acttcgattg cttttcaatc aaatatattt cacttgttgg
     2641 catcaattgg cgaagataaa tagccgcatg aatccctgca aacgacccgc cgacaattac
     2701 aatgttcatc tgcattcctc tttcttataa ggtgttctac tgctaacttc tcaatcagaa
     2761 aacacagtat tcataactcg ctttcaacct tatgatacat agcgatgcag cagaatcaac
     2821 cctctccatc ataccgtgtt ctaggtctgc ttctcaataa tgtactagtg gacctaattg
     2881 aagagtatca tttctgaaga gatgtttcaa aaaatgtact cttataaaag aaaaaaactc
     2941 gccttttacg tacctctgtc caaataggaa acagaaaaga acgaaaagac gagttttagc
     3001 gtgtgtaatg taatagttag aatagacaca tcatttattt ttaattcttt aatgcttcaa
     3061 caactgcttc gcctaattga tgagcagatt gaggattttg ccctgtaaga agccgcccat
     3121 caatttgaac cacttctgaa aaagctgctc cagactgata gtttgctcct ttttctttca
     3181 aagcatcttc tgctaagaat ggtacttcat tcgttgtacc atttgcttcc tcttcttcat
     3241 tactgaaccc agtcactgta cgtccagcta aaatagattt tccagactca tctgtaactg
     3301 gcaacaatcc tacaacaccg tgacaaacag ctgagaccag cccttcattt cgatagattt
     3361 gtgaagcgac tttcccaaga tctattgctt ctggaaaatc ccacattgtt ccgtggccac
     3421 cagcataata aaccgctgca taatcatttg catccacatc atcaggacgt aaggtatttc
     3481 ccaaggcccg atttctgaaa tcttcatctt cataataggc ccaatcgatt tcatccatat
     3541 tcaaaacact ttgcggttct aaaggtacat atccgccttt aggactgaca aaatctgcat
     3601 cgatcccatt ttttactaat acatcataaa aatgagtcag ttcacttaac cacaaacctg
     3661 taggacgctc gatcttttcg tacttttcta catttgttaa tacgatcaat actttcattt
     3721 tcccatcctc tttctataaa aattgcgtta ttgataacgt gctttcaaga aattatataa
     3781 tgggcttggc aaataaagct ctttatatgc ttcttcaaaa ggtttcgtta caaattcatc
     3841 ctcacggttt tccaataatt tctctgccca gtcaggatcg acgatcattt gctgtccaat
     3901 tgctgcaagt tctgcatatt tcaatacctg ctctacatct tcgcgattgc ggatcccacc
     3961 gacaccaact aaagggatct ttccttgaat cgtgttatgg accacttcaa gaatcgattt
     4021 atcgttgtat ttatcagaac gagctattcg atcatacata tttaatgaaa catgcagata
     4081 atctagtttt gactcacgta atcgttcaag aagccagatc gtgtcttcaa aacggatacc
     4141 tggtgtttca tattcttctg gtgagaaacg ataccctacg atgaatggtt ttttagcata
     4201 ttgttcaacc gctgagaaaa ccgttgttaa taattcttca ataaaacgat aacgattttc
     4261 caagctaccg ccccattgat cctctcgtct attcgaatgc ggagagaaaa attgttggat
     4321 caaatacgta tttgctccat gcaactcgat tccatcaaat cctgattcaa tcgctctacg
     4381 tgtggctttg ccaaatgctt ggattgtttc aacgatttct tcttcactca ttgctcgtgg
     4441 tgtttccgca tcaggacgtt ttgctgcaac tgcacttgca gatacaggct gttctccaga
     4501 taaagttgct cgacttgtca ttcgtccacc gtggaaaatc tgaataatcg cttttgcgcc
     4561 ttctccttgg attgctttcg ctagctcact taatcgagga atcatttcat catgagcgat
     4621 gctgagttca cctggccagc cttttccgat atcttggaca tttgcagcac cagtaataac
     4681 agcaccaaga cctttagaac gttgtttata ataagtgatc tcatcttcgg tgatcactcc
     4741 attgaaaaag ctgagttgtg tcgtcatagg cgacagcatc aatcggttct tcaattgaac
     4801 tcctgaaggt aaagtaattg attgatcaaa ttgtgacatt gtcattcctc ccataattcg
     4861 ctctgattaa cctatcgaga atcatctatc attaaaccac tcgattagtt gtacatgcaa
     4921 ctaatttgct taaaaaattt tatttttttt gtaacttatc tgttgcatca ttcattgtct
     4981 gggctaattc tttacttgct tcatttccca aaactgcttt gtatgactga tgaagttctt
     5041 cccagctacc atcgacttcc ttagcaaatt ggcgtccttg ttctgtaaga taaactaatg
     5101 acaagcgacc ttcgctaact gttttgacgt atcctttata ttctaatttt tcaacaaaac
     5161 gagcaatcgt tgatggggcg gaatagagca tttgtgcaat atctttttgt ggtattccat
     5221 ctttttcttt taatatcaat aaaataaaac cataagttgg tgccagacct gtccttctaa
     5281 acgcttcatc agctaattta ttcaacatgc gatctagctt tttcacgcta aagaaaagac
     5341 attcatctaa cgctttttca attggctttt ccatttcatc ccctccattg gcgtacatat
     5401 catagtatga tttttatttt ttgtcaaaaa acacggaata atgtccgcct cctctgtcta
     5461 gatttgaaaa aaagaagtag ccatcttcaa agaatttcgg cttgttttcg acaaaactcc
     5521 ttctggaaca ccgtgaatca gaggttgtga aaaagtcgtt tagttttgag tatgaagaaa
     5581 aattttgtga aaatggcttt ccacattttt gcataaattt ggcttaatac cgaaaaactg
     5641 gcttttgaac accgtttata tcagaggttg tgaaaaagcc gtcctgcatc aagtaataag
     5701 aacagccaaa caaaaatagt tcctcatatt ttgtcgtttt ttgagctaat gccgatggtc
     5761 agctgattgt acaccgttta ttgagaggtc gtgagcctgc cgttgaactc cgagtcacaa
     5821 ggagcaattt caaaaaacag cttctcatgt tttatgacta tggtgacttg tgatcgagga
     5881 gttggcagga gaatactgtt tattcgtaaa gaaggggctg tgacaagact tttgtcacag
     5941 tcccttattt ccttttatta taggatttcg ccatttgatt ggatgacttg tctgtaccaa
     6001 tcaaatgatt ttttctttaa ccgtttcttt gtgccattcc cttcgtcatc ttgatcaaca
     6061 tagatatatc cataacgttt tgacatttgt gaagtagaag cgctgacgat atcaatgcag
     6121 ccccacgacg tataaccaaa taaatctact ccatctttga tagcttcctt catctgctca
     6181 atatgctgtt tcaaatagtc aatgcggtaa tcgtcttgaa cggttccatc ttcttctact
     6241 ttgtctatag ctccgagtcc gttttcagct atgaacagtg gtttttgata cctatcatat
     6301 aattgattca aagcaattct caaaccctta ggatcaatct gccagcccca ttcacttgat
     6361 ggaagatacg gatttttaat tcctccagtt attaagtttc cttggacccc ttcccaattt
     6421 tctggatgag cactgactgc actagacatg tagtagctaa aagaaacaaa atcaacaggg
     6481 taggctgcta agatttcttc atcacctact tctattttta agttgacacc tttttcttca
     6541 aacattcgtt gcgtatacgc tggataatat cctcttgctt gaacatcact aaagaacaaa
     6601 gaagttcttt gctgctcaat tgttttttga atatcatccg gatcacaaga atacggaaca
     6661 agaagctgat agctgatcat acaaccgact tgtgaacctt tgataatttc atgacaagct
     6721 tttgtagcta aagcacttgc tataaactga tggtgtgcac cttggtattt cgcttgttcc
     6781 aaatggggat ggttttcttc aattactcct acgctaacaa aagggtgatg ttttacacag
     6841 ttgatctcat tgaaagtcat ccaatatttt actttatttt tatagcgttt gaatactgtt
     6901 tcagcgaaac gaacgtaaaa ctgaataagc tttcgattag gccatccacc atattctgta
     6961 accaaatata acggcatttc ataatgggaa agagaaacga ctggctcaat cccatatttt
     7021 agacattcgt caaacacttg atcataaaaa aataatcccg cttcattggg tgtttcttca
     7081 tcaccatgag gaaaaattct tgtccatgcg atagacatcc gatagcattt aaaacccatt
     7141 tcagcaaata aacggatgtc ttctttaaag tgatgataga aatcatttcc gtgtctttta
     7201 ggataataat acttgctgtt gggatcctgc gccgctttta cttgctgatg cgtcatcata
     7261 tgccattgat ctgtccattt ttctttaggt aaatcgctgt cgaatgtata cacatcagac
     7321 acagataatc ctttttcatc tacatcccat gcaccttcac attgattggc cgctgtcgct
     7381 ccgccccata aaaatccttc tggaaatttt ttcatatcga tccccctaaa cttgctagtt
     7441 ttcttgtttg attatttttc ttatgactag taatgatatt tttgaatcta ctactatttg
     7501 taacaataac cattgtggtc ggatcatatc cctctttgat taattcttgc aaatctactt
     7561 ccatcaataa atcgccttta cgtacttttt ttccatcaga tactgcactt tgaaagtatt
     7621 tgccatttaa attcactgta tcaataccga tatggatcag tatctctgct ccttcttcac
     7681 tgactacccc aattgcatgt ttcgttggaa aaactgttga aacttccccg tttacaggac
     7741 tgtagatttt tccgtccgtt ggaatgatcg caaacccttc tcctaaagat ccactagcaa
     7801 atgttggatc gtttacttca gataaatcaa ttatttcacc tgctgcagga gtacttaaca
     7861 ctatatcctt tgaggattct tcttcgattt catccttgta gccgaaaaga aaagttagca
     7921 tacaggaact gatcatcgct aagaaataac tagcaactag ccaaacaaat ccttcaccaa
     7981 tataaatcgg gaaagacaaa atacttcttg tagcaactgc caaagcaccg gctcctgcgg
     8041 caccagctac tccaccaaat atagctccac atatacaagc aatgtaaaat ggtcgtttat
     8101 atttaagagt cacaccataa acagcaggtt cagtgattcc aaataatgct gtgataaatg
     8161 cagataaaga taaacttttt actttcttat ttttcgtttt caaaaatacc ccaagtactg
     8221 ctcctgcttg cgcagctacg gcagtcatac atgctggacc tagcgtatca cgaccgtaga
     8281 aagaaagatt gttggtcatt actggtacaa gtccccaatg taacccaaaa atcactaaca
     8341 cttgccaaaa tccgccgata acagcaccag ataaaacagg gctaaagtta taaacagcta
     8401 aatatccagc tgcaatcagc tgacttgcat aatctgcaat tggacctaca actaataaag
     8461 taattgggac catcacgatg atacaaatga atggtgtcaa taaatttcga atactttcgt
     8521 gcaagctttt gcgaatctta ggctcaatat aagaaagaac ccatacagct aaaattgctg
     8581 gaataaccgt acttgtatac cttgctagaa tcacgggcat ccctagaaat cgcaaggtta
     8641 tgctatcact atatgcactg acaatcgtag gataaacgag agctgctgca ataaccatag
     8701 cgatgaaacg atctgtattg aacttttttg cagcagtata tgccaaaaat ataggcaaga
     8761 aatagaaaaa tgcatcagct gctgcattca aaatgatata agttcctgat tcagtagtta
     8821 ataaattcaa actcgtacat aaggcgagta ctcctttgat caaaccactt cctgccaaga
     8881 cgcctaaagt cggggcaaaa acagcggcga tcatgtctat aaaagagttt aaaatgcctt
     8941 ttttctcagt tcctctcttt ccactagatg catcatcttc tagtttcatt tgtttggata
     9001 atacgtcata cacttcattt actgcatttc ctactacgac ttgaaacatt cctccagctt
     9061 cgactacagt gatcacacct tctagttgct ctaccttctc cttatctgcc ttcttattat
     9121 cttttagggt aaagcgtaac cttgttgcac aatggtaaac attgctgaca ttttcttcgc
     9181 ctccgacaaa tcggacaata tctttcgcta attgtgaata gtccattttt acttccccct
     9241 tgttaattaa tgccgtagca atccttctta caagataagt atagttctta gaaaacggtt
     9301 tctccattca attagtaccc cttataatag cgaacgaaac tgtacaatag tgtgtatggt
     9361 ttacttagcc gagcaaatca ctcactgctt gaaagaaaac aagtatatcg tttctcttat
     9421 agtcaaagga gacaaaaaaa acacgcttga tcttcctttg ctgatgtata agtggtatct
     9481 caagctgata ctctgtgatt tttgtttcta cttggctttt gataagacag cacaacaaag
     9541 tctcggcatt taacggaagc ataactaggt cagaactagg agagattagt tgctgaatgg
     9601 aatctatctt tttatggatt tttatttgat tttcttcttt gtagtaatga tcaagcagtt
     9661 ttgttttctt tttttcttca actatggtca gcttttcacg caaaatctga taaatctccc
     9721 aagattcaaa ctcatctaga acactaggta gataaatcaa atcatcccac tcaaaaataa
     9781 gctcttgctc agtagtcaaa attgcatcat attccgttat tttgtttgat tgatttttga
     9841 aaagatacac tggttctatc cggcattctt tgatccacat gttcatactt tgttgaatct
     9901 gtttttttag tgtattcaaa atactaattg gctggttact aacaatcagt atctcccctt
     9961 gttttcgaaa aggattcaac gcttcggcaa gatacaaaac aaaataagcg ctttcagcta
    10021 aattgggacg taattgaaaa tgtattggga agaaacgccg aattaaataa atttctaaag
    10081 gataacggga gatgatcttt tgtacaaaat gattcatggt attatgtccg gcatccatcc
    10141 tcgttttcat ttgtttgata tgggttagca aaaattctgt ttcatggaac aataacgaaa
    10201 caggaagatc taaaaaacga ataatcgttt gctcaaatgc tcttagattc ttctctattt
    10261 ctttgttttt ttcatcatta atcatcaaat aatttaactc taatagtcga ttttcaattg
    10321 cttgtttttc ttgattggaa aaagaatagg gtagttctga tgataacttt tttgtgaggg
    10381 tctctaccat cggaataaat aatgtatacc tgtttgtttt ttctaaaagt tcttctcttc
    10441 gaagaatggt caaaacgaga taccgagaaa atcgtatgaa gtcctctcct gaaacaatat
    10501 aattatatga tataagaacc tcttttaatg tcttttggac agtcggcatg atattggaaa
    10561 aaattgtact gatttgttga ggcaaccttg cgtgttggat caccgtttct gttccaacaa
    10621 gagataagaa aattcttttt gaattttctt cagcaatgat ttttagtcct tttgtcgacg
    10681 aaacttccag ttctaattta ggattgcgct cgatccaacg caaaatcgtt tttatttgta
    10741 atgatacggc tgtttttgaa agaaagaaaa tgtctgctat cttttgcata ctgacatacc
    10801 ctttttgaaa caataagtac gtcacaatag ttgaagctcg gaaatccatg ctggaataga
    10861 ttttatgttt cgtcacctct tgtatgattt gctgtttagt ttcagattca attgattcta
    10921 aaaaataacc tgaacgttta tttgactgga taatgatttt cttctcgtag atagcaagca
    10981 tatttttgat ttcagtttgg atcgttttct tgttggtacc taaagcttca gctaattctt
    11041 cgcttgttat ccaatgattc ttatttctta atactacaag aatttggagt tgtcgtttat
    11101 ttaagtccat ttgcttacac ctcctctttg ttataaacat acaggaaaaa cctctcaaat
    11161 gacaatagtc acttgcagag gtttaaaaat catttgaaga aagatctata ttcaaggttt
    11221 ttaccattag actggataat ttgtttataa tattcaaaac tatctttttt caatctccta
    11281 aagcttccgc tcccatcatt ttgtctatcc acataaataa agccatatcg cttcctcatt
    11341 tcccctgttc ctgccgatac gatatcgatt ggtccccacc ataagtaacc aatgatttgg
    11401 caaccatctt ttattgcctc agcaatttcc tgaagatgta tttttagata ctcacaacgt
    11461 tttggatcgt ggattcgatt caattcatct ggtgcttcat ctagtccaat gccattttca
    11521 actatgaata atggtaattc ataacggtct gtcaattcat tcatgacata acgaaaccct
    11581 agaggatcaa ttggccaact ccaaggttcg ggagattttt ccttcaaaaa gggattatcg
    11641 aaaccatttg ctccgccagt atctacgcgc atttctgctt cttttttata gacagcgcta
    11701 cgataataac taaatgcaat ataatctgcc gtgttagctt gcaagcactg ttcgtctcca
    11761 tctaacatta ctggtgcaca gtcttcttcc ttccatgtcc tgtaaatata agctggataa
    11821 tgccctcggc acatgacatc ggagaaaaac catgaattgc gtttaaattg cagtgaacca
    11881 aatacattct ctggatcaca gtcatatgca taagtcgcta atgcagaaaa gctacacata
    11941 atacccatct tagcatttgg atcgattttc tttaataatt gagtagcacg ggcgttcgct
    12001 agaaacatat gatgacaggc ttgataaatc actttttttg ttagtgtagc aatgggttca
    12061 agtttatctc tagcagggaa tgtaaccaat ccagcagctg cataaggcat acgaaaagca
    12121 ttattgatct cattaaaggt catccaatat ttcactttcc cttggtagcg ttcaaagacg
    12181 gtctgcgcat aaaattcaaa aaagtcgatt aattttcgat tttgccaccc cccatactct
    12241 cctaccaatg ctaagggggt ttcataatga ctaagagtaa ttactggctc gatatcatat
    12301 cgatttaatg tattgattag tctgtcataa aatacgagac cagcctcatt tggcttctgt
    12361 tcgtctcctc ttggaaatat tcgtgcccat gagatgcttg ttcgaaaagc ttttaaacct
    12421 aactccttca gtaaacgaat atcctcttca aatcgatgat aaaaatcaat cgcttcatga
    12481 gaaagataat ggagtgattc gtctaaagca atctcccaaa tctttttttc ttcattccac
    12541 tgaatactag gatgctggtt catgatcccg ataagcgtat ctgtcacatt aggtaatttg
    12601 ccgtcttcca accatgcacc ttcacattga ttagcagcta ctgctccgcc ccataaaaaa
    12661 ttttctggaa atatttcctg atccataact atctctcctc gctatcaaca atttcttact
    12721 tgaagtatag ctcgattcat caaaacaaga tattctaact atttcggcag aagagaagaa
    12781 aaaaattgaa tgctactctt catttttaat atttatatat aacaaataca taagacgatc
    12841 cccgaaagaa aacactcgtt aaccatctaa gcaaacgatt tattttactt ccaacacaaa
    12901 tgcttcataa ggtcgtaaat caactttgcc gtctggtaac tgtttcagag gataatttga
    12961 cacaataacc cttaaagctt cacgtggaac agtgaacgtc tgcttttcac tggacatatt
    13021 acaaataacg agccattgct ggtcttcata tatgcgagaa taggcaaaga catgctcagg
    13081 cgtcttttcc actaattcat agtctcccca gacgatgatg tcatgttttc tacgcaaatc
    13141 gatcaatttc ttgtacatat aaaaaacaga atcttgatct gctaacgctt cttctgcatt
    13201 gatttcttta taatttggat tgactcgaag ccatggaatt ccagtagtaa acccagcatg
    13261 ttcacgggcg ttccattgca taggcgtcct agcattgtct cttcctttcg cattgattga
    13321 ttccaagatt tcctcttttg taaatccaga agaaatcctt tcgttataca tattgattgt
    13381 ctcaatatct tttgcttccg cgatatcatc gattgggcaa ttcgtcatgc ctaactcttc
    13441 tccttgatag atataagggg tgcctttcat catatggagc aagatagcaa acatcttggc
    13501 actttctttt cgatacacat cgtcatttcc ccaacgagaa acaattcgcg gaagatcgtg
    13561 attattccaa aataaactat tccatccttc atcacctaaa gatgtttgcc atttggataa
    13621 tacttttttc agttcagaga tactcaaggg tttcaaatcc catttgtctt tgccttcttg
    13681 ctgatctaat cctatgtgtt cgaattggaa gaccatggat aattcatttc ttttaggatt
    13741 ggaataaagt ttagcgattt cggtagttgc tccccatgtt tcgccaacag tcatcagatt
    13801 tttgtcacca aaagttgctt gattcatttc ctgaagatac tcatgcagtt taggtccatt
    13861 acttgtgatt tctttatccg gttctttgcc aatcatatcg atcacgtcca tacgaaagcc
    13921 cccaatacct ttatcgatcc agaaattcat catttgataa atctcttggc gaacctgttt
    13981 atttttccaa ttcaaatcag gttgacgctt gctatacaag tgcagaaaat attgccccgt
    14041 tttttcatcc aactgccaag cagaaccact gaatattgag cgcaatctat taggctcttt
    14101 tccattgacc ggatcacgcc aaatataata gtcccgataa ggattttctt tatttttacg
    14161 ggcctcaatg aaccacggat gttcatcaga agtatgattc accaccagat ccatgatgat
    14221 acggatattt cttttctttg cttcttcaat cagttcttcc atttcttcca tcgtaccgaa
    14281 ttctgccatg attgcttgat aattactgat atcataccca ttgtcatcat tcggagactc
    14341 gtatactgga gaaagccaaa ttgcatcaat acctaaatac gccagataat ccaacctgga
    14401 aataatcccc cgcaaatctc caataccatc ttgatttgaa tcttgaaaac ttcttggata
    14461 gatttgataa acaactgaat ggtgccacca tttttgttcc atgattttcc tcctataaat
    14521 ggatgtttct attttttgct taatcattta ttttagtctc tctcactaaa aattgtaacg
    14581 ctttcaaata gctattgcac cttgttatcg gtaacagaat aaaacaaaaa aacagctgag
    14641 gaaattccct caactgtttt ttgaaaggat tgatttatgc agggaagaat gctgatttca
    14701 caacttcttt ataaacggtg ttcactcagc tgtcccttgg tattagctca aaaaacggca
    14761 aaatatgagg aactattttt tcgttttttt ctctaatact caggccagaa cactgatttc
    14821 acaacctctt tataaacggt gttcactcag ctgtccctcg gtattagctc aaaaaacggc
    14881 aaaatataag aaactatttt ttcgtttttt tctctaatac tcaggccaga acgctgattt
    14941 cacaacctct ttatttcaca ttcaatatcc acatgaatcc cacaaataca aagaacagac
    15001 catacatgat tggagatact tcttttcctc ttttagctgc taacatggta attggataga
    15061 agataaaacc taatgcaatc ccatctgaaa tactataagt caaaggcatg ccgatcaaga
    15121 tcaagaatga agggatcgca atctctaagt ttccccattc gatttgtcgc aaagattgtg
    15181 ccatcaatac tcctaccata atcaatgctg gtgcagttac ttgtgaagta acaactgata
    15241 gtaatggaga aaagaacaag cctaacaaga agaaaatccc tgtagtaata gctgtgatac
    15301 ctgaacgtcc tccaacggcg ataccagctg atgattctac aaatgcacca acaggagaag
    15361 taccaaataa ggaacccgca agcatagctg ttgaatcaga agctaaagct ttccctacac
    15421 gaggcatctt gttgtctttc atgaaacctg cttggtttgc taatccaact aaagttcctg
    15481 ctgtatcaaa gaaagtcaca agcaagaatg tcagcactac gacccacatt tgaagagaat
    15541 tgatgtcccc tacatgtttt aatgcaacta aaaatgttgg tttcaaactt ggtgctgcgg
    15601 caacaatttc agaaggcatt ggaatcaaac ctgtgaccag acctaaaatc gttgtagctg
    15661 ctatcccgat gaagatgcca cctggtacac gccgaacaag caagatagca gttacgacta
    15721 atccaaaaat cgtcaaccaa gtagaaccaa cgttgagtga tcctaatcct actaatgtag
    15781 attcattcga aacgatgatc ccaccttcgc tcagaccaag gaaagcgata aatagaccga
    15841 taccgccaga aatcgcatat ttcaaatctg ctgggatcgc atcaatgatc atttcccgca
    15901 gcttgaagat tgtgatcaaa ataaaaatca aagaagcaac gaatacgccg gctaatgctg
    15961 tttcccatgg aatccccatt cctaaacaaa cagaataagc aaaaaaagca ttgatcccaa
    16021 gagcaggtgc cgttccaatt ggataacggg ctaatacccc cattaaaaca cagccgatcg
    16081 cactggctaa tgcagtcgca gtaaacactg ctccttcatc cattccagaa gcacctaata
    16141 cagttggatt tacaaataag atataagcca ttgagataaa tgttgtaaat cctgccagta
    16201 cctcacgttt taccgtggtg ttcaattttt ctatttcgaa gtaggaaagt attttttcct
    16261 tcatcttcca tccccctaaa tccgaacatt ataaataaag tccgttaaaa tttcgattta
    16321 ttaataaaac aacgtgtttt atcatatact aaaacaaaac ataagtaaac aaaaaattcg
    16381 gattttaagc aaaatccgaa cgttccatct gattaaatcc ctttatctca aagttcatga
    16441 acattcactt tggattacaa gaatttcttt attgtgttaa taaagtttct tcattctagt
    16501 ctcaaaaatc aatagtgtga tttgatttta ccatttcgtt tctgttcttc tttgatgaat
    16561 tcttttgtat cgacaggatt cagattattt agtccgccaa aacctgtatc gaccattttt
    16621 ccccagcctt ttgtcttctt ttttcctgat ttgagattct ttgctcgaag cttctcacta
    16681 tgttttgctc gccgatactt caaataacct tctaatcttc ccattagatc cactgttttt
    16741 cctccttttt attcaatcag acgactcagc ttttcctata acaaaataat tgccttcagg
    16801 gtcagaaaaa ttgaaagtca agatgccatt gttatctgtg atttctcctg gttcttccat
    16861 gttctcgtag atagtttcaa attcatccgt aaaaaatact aaagaaggcg gtggtcctaa
    16921 tacctctggt gaaaacttct gcacaaatac ttttgggaat acgcctaatt ctaattcttt
    16981 tgagatgctg agtacaatac tcttaaattc ttctggcaat tcgattgttt cagaaatttc
    17041 tgcaccaatt ttttctacaa agaatcggca aatcatctct acgtcattga cataaagcat
    17101 cacacgtgtt ttttgtatca tcatttgacc cttctttcta tttgattttc tttcctgtct
    17161 ataaactgat tccattatat cacgaaagcg caaacacctt gtgtttgata ctattcaaga
    17221 aaaataaagc aatagtttct aggattgctt tcctccacaa acttttttta caaaaaaaaa
    17281 aaacggcaat gaatgccgct cttcttattt cgattctttt ataattctgc ttctgattca
    17341 tctttggtta gtgtgtgtct aacactagtt acgccaaatg tttgatcgat tttttccttc
    17401 aacgcttcga ttgcctgtgc gccacgtgcc gcttgttcag cattgatgga ttcaatgacc
    17461 agcaccgctg cttgtgtttc ttccgtcagc attgttcttt gggcttcacg ccagttgaga
    17521 cagcgacaga tcgctccttg ttcatcatag tagatgattt cttccggaag tgccggagca
    17581 tcggaatctg cacctaaagg caggaatgat tcgccacctt gggcttttcc taaatgcaga
    17641 tttcctgcga tcttttcgat atcttctccg ccgcaaggta cgccaaaaga caaggaaata
    17701 ctattataga tatctacaag cggattgatt gggaaaaatt cccgttcttg atttgcacgt
    17761 ttcagtaaag cttcaataga agaacgtgca ccttttttag tcttgaatgt ggtaaatgcc
    17821 tgacgccact gagcaatgac ttcgttttgg ctgaatgttt cttctgtaag gtaatttctt
    17881 gcttcttttg ctgcctcatt caataattga tgatacgttt cttcactttc ttttgttacg
    17941 tggttatcga taccatttac ggataagatt tcgatggttg cttcaggaaa tacttcccag
    18001 aaattggtat ccactattac ttttttcatc tgttcacacc tcttgattag aataattgct
    18061 tgatgaatag atagtctgtt tggggatcat ctcccatata aaaaacatgt tcgctgtatt
    18121 tgataaatcc ttttttacga taaaaagcga gcgcaacatc gttgtgttcc caaacaccga
    18181 gccaaatggc tgttttttgc ttttcttttg ctttttcaat agctgtatct agtaaaaatg
    18241 taccgatacc ttgatgttta aatttttttc gtacatagat tcgctcgatt tctaaaaaat
    18301 gatcagccat ttcctcagat tgactcgttc ctgtatttag tttcaggtaa cccgcaggct
    18361 cttgatcgat actcactagg aaaaaatcgg tttctttatc ttttagctct tctgtaagtt
    18421 tttctagtgc ataagcttgt gtcaagtaat cagctaaatg ctccggtgaa ttatcttttc
    18481 caaatgtatc tgtaaatgtt tcgatactaa tttgttgtag taagtctaca tctgaaggcg
    18541 tcatttttcg aatttcgtat ttattcacgg cttcttcctt tcaatagatc cttttattgc
    18601 cttttttgac aaaattccaa tcttcgctaa cattcttgca tactttattt aaaagatatt
    18661 ccaattgttt tgcttcttcg tcagaaagac catttaatgc gactgcattg gagtagttgt
    18721 tttctcgaat gatgaaagga tagatttctg ctcctttttt agtcggatag agtttcttga
    18781 tttttttatt ttctttatct tccaaacgtt cgatcatccc attggattct aatttcttga
    18841 ttgctcgggc agtcgttgta cgatctactt tgatcatttc tgctagtttt tcttggatga
    18901 ttccaggatt ttcgcaaata cgtactaaat aaagatactg tcctcttgtt aaatcgactt
    18961 ctttgaattc gatattgcta atagaatcta gcgctcgggc aatcattccg atgtctcgaa
    19021 ggatttcagc catttcatcc cacctttatt ttgtttgtaa gactagttta cctctttttg
    19081 ttgcaaatgc aactattttc aataaaaaaa tcaagttctt ttttaataac tagtaaagta
    19141 tattcttttc ttcttttacg aaaaagctca atcacaatag atctggtttt tgttttctaa
    19201 aataagtaac tgtttcttcg ttgttcgctt ttctgatagc tttggcaact cttctatact
    19261 aaaaaatccc atgttggaag tttcattatt ctcaataaac ttggcctctt caccgtggat
    19321 cgcacaagca aaaacaagtt tataatactg aaaaagctgt ggaatatctt tttgtttatt
    19381 cgtatcaaaa actgctcgca actgagtagt ttctactgtt aatcctgttt cttcatagac
    19441 ttctttacgc acattttctt caggagacaa tcctatttca gcaaatccac caggaagtga
    19501 ccattctttc gtccgtttat cttcgattag taggaccttg ccatcttttt tgatataggc
    19561 acgaacatct acttttggtg ttggatgtcc ttctccaatc gttgtgagcg cttttatttc
    19621 ttcgaaagtt tcttttccta tttcactgat cagttccaat gaaatcgtcc ggatctcctc
    19681 aaaacgctct ttatcaaata catcttttcc ataatataga ccagcttgag aaagtgcgag
    19741 tagtcttttg tattgagcca acttcttgtt cattctcttc ccttcttttt tagcaaatgt
    19801 atgactatgt aatcaagtat agaagactgg tttaattacg gcaaataaat ataatgaaat
    19861 ctgcaataaa taaaagaatg ttaaaaaact cgagaacaaa agacattcga cttctctggg
    19921 gctgtgaata aatggcgttc aaaagcttgc atctgcggta ataagcccca ccaaacgaaa
    19981 aatatgacga gctattcttg tctggctgtt cttattaatt gaggcaggac agctttttca
    20041 caacctcccc actaaacggt gttctcctac caactcctcg gtcacaagtc acaatattca
    20101 tcaaacatga gaagctgttt ttttgaaatt gctccttgtg actcggagct gaacgacagg
    20161 ctcacgacct ctcggtaaac agcgttcaaa agccaatttt tcgatattaa gccatttctt
    20221 tgttacttat tgtcattgat ttctttttct aatttatcca atagtggagt gtaatccact
    20281 tgagtatagt ctgttacatc aaattcatat agtgttccta actggaattt gtcatatttt
    20341 cggtcggcga tgatcttttt gaatgcttct ttcgtaatat gattggtccc tttcgttcga
    20401 tcttccaata cgtcaccata gtgataatga tccatgatat agctcaaatg acgatctgat
    20461 tcacgatcac gtttgtaccg acgttgacat aacacttcaa agtctgctgt taggcgaatc
    20521 gtgatcacct gatatccgta tctatctgct aattcttgga gtcgtgcttt ttgcttatca
    20581 ctaaaaggat actcggacac aatcactttc tttcctgctt ccatatacaa atcaagtgct
    20641 ttgtaataga aaggataaac tttcgtcctt tcttgttccg cccgttcttc caaattgtta
    20701 aacccgatgg attctgcata gtattccttg atttcatcga gcgtgatggc atacatatct
    20761 ggaaatcgtt cccgtagttt attcataaga tatgtttttc ctgtaccagg actaccagcc
    20821 agaagaatga aatacttttt catctcattt atttcctaag ttaattggac tttccaagtt
    20881 cataaacagt ccaatcttat gcttgacaat ttcttttact gcttcatttg ctggaatcac
    20941 attatggcga agagacttgt tgatttctgt ttcactaaag gtttctttag ctactttcgt
    21001 ccagttgact aaaagctccg ttccaacatt gaatttgcgg atcccgttat tgatcaattc
    21061 aggataatct tcttctttca cacctgttcc tccatggata actaggggga catcaactac
    21121 tgcatcgatt tctttcagca agggaatgtt tacttctgtt tttgatttga attgtccatg
    21181 attcgtaccg atagcaatag ctaatgcatc cacgcctgta gcttctacaa atttcactgc
    21241 gtcttccggt cttgtatata ccttgtcatt ttcagcgaca tggattcctt cttctgtacc
    21301 gccgattgtt ccaagttctc cttctacgga aacaccgtgc gcatgggcat attctactac
    21361 tgcttttgtc ttcaggatat tttctttgaa aggcagatgg gacccatcat acatgacaga
    21421 agtatatccg ctgtcgattg cttctttgat atcatcaaag tctcgggcgt ggtctaagtg
    21481 caagactgca tcaaccattt cttcttctgc aatagctgtc attacattga ctaacacatg
    21541 catgccgata tatttagcgg tatcgacact tgtttgaata atgatcggcg aacccatttc
    21601 ttttgctgcg cctagcatag caggaagcat ttctaggtta tgcgtattaa aagctccaac
    21661 tgtaaaattc aactcttctg ctgtttttgt tacttctttc aatgttgtat acatctttta
    21721 ttgccacctt atctttttat ttgtttcctt tgatcgttac ttgttttgca atttgtcgca
    21781 tctcatccat tttgcccatg tagtaatcga tcccttgatc atcaccagca cgagcagcat
    21841 ctgcacgctt ctggttatag aggctcatca gttttttata cccatctccg atcgtctgtt
    21901 tattttccaa actcttttct aaatattgga tggcttgatc gttcacatac aattcagaaa
    21961 atttaagtcc aacttgttca taaagtttcg ctaatttatg cttatcttgg gtagtggaaa
    22021 tctcttgttt caattgtgta atctctgatt gaatcttttc ggtcgcttct ggcgataggg
    22081 ctttttcttc ttttgccaga acttttttcg gttctttttc ttttttctta aaaaatccaa
    22141 acatcatttt ccctactttc aattaaaatg ctttcaaaac tgggcttaag atccaggcat
    22201 ataagagtgc tgcggctact gtatctacat cggttgctgt agcattgatg aatcccatcg
    22261 aattaaagat cgttactagc aaagcgggca ataatgtaat aaagaaacca tgagcaatac
    22321 cgccaatgat cgctcctcgg cgtccgccaa ctgcgttgcc aaatatccct gctgtccctc
    22381 cggcaaagaa attggttagc ataccaggta aaatcatcgc taagccgaac atgggcaaag
    22441 taaacatggc aataatcgta ccaatcgttg tcgtaatgaa acctaaaatg acagcatttg
    22501 ggctataagg aaagaaaact ggacaatcta aggcagggat cgcatcagga actaatttca
    22561 tagctatccc tcgaaatgcc ggcacgattt cacctaacag tagacgtaca ccagctagca
    22621 atacatacac acccactacg aattggatgg cttgtaaaaa ggcaaacatg atatagtttt
    22681 gatctcctga taattcagaa gcgaatggtt cgcctgcaaa taaaacagtg atgatatata
    22741 gcgggaccat cacgaccatg acagacaaat aagtatcttg cagaaattcg aaatgtgtcg
    22801 gcaacttgat atcttcgatc gactttttat tttcgccttt ctcaccaaaa agcttagcaa
    22861 caccggcttc aaataagtac ccgatcgtac aaaaatgacc taaagcaata tcatttgatc
    22921 cagtgatttt acgaatgatc ggttgcgcta ctgccggcat agcaatggcg aaacaagctc
    22981 cgataaaacc accgaccaag atcaatacga tccctcgcag accggcaaaa tagccaaaga
    23041 ctgtcgtcat cgttgccatc cataaaattg cttgaccagt taaaaaaatg tacttccatt
    23101 ttgtgaaacg agccaaaata atattgaata caaaaatggc taagaacgtc agtgcaatat
    23161 ctctacctaa acccaaatca ttcattgctt gaccattgat agattcaata gaagggatga
    23221 tcccttgcat atgaaatcct tcagtaaaga ttttcccgaa ataagttaaa cttccaacga
    23281 tgatgctcga accggcactc aacacttgaa atcctaggag tgtctttaac gtgccagaga
    23341 tgacttgtcc tgctgatttc ttctgcagta gtagacccaa catagcaatc aatgcaatgg
    23401 tgatcgatgc ctgagtcaaa atattttcga tgataaaatt gataacgccc atgtttttac
    23461 cctcttttct ttttgtattt agataactcc cttatcttgc agaactgggg tgattttttc
    23521 ttcaatttca ttttttgaaa caatattttt caaatagatc atcgccgttt taggatcaac
    23581 tgcgaacttt tcgaactgcg aacgaaaatt ttctgctgtg atgatcacat ctggtttcat
    23641 ggaaactgcg gaagaaatat cacaatggtc cagtttggct tctactccta atttgttcaa
    23701 aacatcctca gcagacatct gtgctgcaaa actacttcct aaacctgcac cgcatacgaa
    23761 taaaatgttc aatttgttca tctttgttct cccccgtatc atttattatt tctcaactac
    23821 tgaaaaaagc agttgtttga aatcttcgat atttgtgcat tcaatcaatc gatccaactt
    23881 ctcttcgttg ttgatcaatt caattagttc tttcatgatg ttcagatgcg agtaggagtc
    23941 tactgctgcc aaacaaaaaa taatctttac tggatccatt tcaggattac cgaaatcgat
    24001 tggttgctca atcgtagcta cgctgactcc taatttattg accccatctt ctggtcgggc
    24061 atgggctagt gccatgtgtt tgccaatcac gatatacggt ccgtaatcat tcactgcacg
    24121 aatcatcgct tcgacatagc gctcttcgac aatgttttct ttaaccaatg gtctagcagc
    24181 tagttcaatc gattcctccc aagtagtggc tttttctttg atcatgatat tgctgtccgt
    24241 caaaatatct tttaacatag gctggatctc ccttttgttg atttctaaat tgtttgtttc
    24301 gaataatttt tctaacttgc catagatgat tcgatctact cggccaccac tttctttcat
    24361 gacatctatt atttgagtga ataactccgt cgattcatga ctgttaggct ctaaccgctg
    24421 tagttcttca tttcttttca aaaagtcttc tataaccaag atattcgtct cagttaaaat
    24481 cggatcgaca actaaaagcg gcttatttcg ataattcaat tgaaaggtgc tgaaaaccag
    24541 attgacatcc aatttatcta ctaattcgat ttcttttgaa cttaaaactg cagtaatctc
    24601 gatctgagga aacttttctt tcagattctc tgctaacaga tttccagtag ccatcccatg
    24661 gttacagaca acgactgatt tataaacata attcaagtct cgcttgatta cactggcgat
    24721 cgtcgaaaag tggatgacaa gaaaagctat ctcgtcctcc agtatcttgc caccaattac
    24781 ttcttcgatc gtaggaacaa aatgagtgac tgcgcggtag attgggaaat aatttgttcg
    24841 gatgttttct ttcaatggat tcatgacttg gatatgattc ttgattctta caatcagtgc
    24901 agccatgtgc ttatagagat tttcgaataa agcttcttcc tttagatgga aaggtacgtg
    24961 tgtctcgttc tctacaaatt ggatcaattg gatcgttagt aattgagctt gtacccactc
    25021 gattgaattg gagatatctc tagcattgaa cgtctcaata gtgaaacgaa gataatttct
    25081 ttctacttgc gggacattgt ccaattgaaa ttcctcgatc atgtggtcaa taaatgcaga
    25141 aaagctactg tcttttattt ctgtattttt ccaattaatg gcagagataa attcatgttt
    25201 cctgattcgc tggatccaga tcaatgtaaa aagcaggatt tgttttttat aaatatcatc
    25261 ttcttgtttc aacaacttat tggaatagat ctcctgtatt tttttgattt ccaccaaagg
    25321 aaaataatgg agaaagattt tttgagccgg tgttaattgt gcatcttgat tcataaagaa
    25381 gtcggtcatt cccagattct gattgataaa atcatagatc atcgtacgga tagatcgttc
    25441 tgaaccttta taaaccagtc cttgctttcc ataactgacg atctctatcc cataactgac
    25501 cagtgttgct cgtaaccgtc tcatatcttc atctaatgta cttttagaga tttgaaattc
    25561 ttcttctttt ttgtttagat ggatcgggat tcggctgtaa gcaacggcta aaactagatc
    25621 aaaaatacgt tcgcgccgat tcaaaaagcc gtctttcaca tcttttattt cagtttctaa
    25681 tcgagcttgc ttttcctttt ccagcttcaa caaaaaaact ttgttgcgaa ttgtacggat
    25741 ctccggaagc tttttactgc tcaaccagat attcgcttca agaacttcct gacggatcgt
    25801 ccgctgactg acattgaact cacttgctag atgttttgct tttactggat attgggctgt
    25861 catgattctg tgaagtattt caattgttct tgttttcata gaaatgatct ccttctgatt
    25921 tcaagtatat ggttccgctt acataataaa aagtagatat ttctgccact tgaagtggaa
    25981 agaaagtaag caaagcaaac aaaaaataag ccatatgctt tttcatctcc tcaacgaaaa
    26041 agacatacgc ctattattac aaaaattttg cccatctata caaaaaagag gttgtgaaaa
    26101 agctgtcctg catcaagtaa taagaacaac taaactaaaa tagtgcttcc tatttttcgt
    26161 ttggttgttc ttattatcgc agatgcaagc ttttaaacac catttagtgg agaggttgtg
    26221 acagagatgt tcagccttga gcgataagaa gcgattttga aaaatcgctt cccatatttt
    26281 acaaaatcgt gccttattgc cgataggcta tctctggcac actgtttatt cagaaaaagg
    26341 agctgcgaca aggcttttgt cacaactccg ttcataaata tttcctgtca atccagcata
    26401 atcggctgaa taaaatcggc ttgccatctt ttttctttct cagaaaaatc aaaataccat
    26461 atctgagaaa aatactgtcg ttgtgtctta cttcctgaga tcaatcgccc atcttttcga
    26521 tcgactgaat aatcacagca tctgaaatca attcggatgg caaaactatt ttcgtggatc
    26581 tgtcgataat ttcctacttc ctgaatagtt atttttttag tatgatttct tacaccgtct
    26641 gctttgtttt tttgcagaac tttctgatgt tcggcaaaca gcttgtctgt ataacagctt
    26701 ctggcttttt caggagtttc ttcttcccaa gcggattgga tcttcatgaa tatcttttgg
    26761 atttccagaa actgtgcttt cttttcttta gccgttcctg acatgcggga caatatctcc
    26821 gctttcagcc ttttcttttg tcttttattc ttgacgatag aaaaagcaga aacccctcct
    26881 actgacaaaa cagctaatgc aagaaatccc gtttgctcat aactagaagg acctgtacct
    26941 ggaccataat acccataaga atctctgccg taagaacgac tgccaaaata actgccccca
    27001 tatgaacgac caccaaatga acctctgccc gaacttcgac cgctagatgt acctccacca
    27061 tgaccacccg cgacagcgaa tccttgggtc ggcagagcaa acaaacatag gcccactaac
    27121 aaaatgattg ctaaataatt gatttttttc attgtcttcc tcgttatctt ttattacaaa
    27181 aaactatttt tatgatagcc ttgctaaaag gaagagtcaa taaccagatg cataaactgt
    27241 ttttttagca attttgtctt atttagtcat tgagtaacat cggacaaatg tatgtgtcgg
    27301 cagtccataa ttcctctatc ttcagcatga acaagcttgc agacaagaga tctgcgcttc
    27361 ctccaggaga gatttggttt tcctgacaaa agtgcaacag tgctgtaaat ggacgatttg
    27421 gtaaagcact ttgactagca gcatgagcaa gtttttgcat ttccactaac gttttctgat
    27481 cactgcgatg caatacgcat gtgtcatcta gcgaactcat caaaactaac aacatatgca
    27541 tttgtttctg tacatatgaa tcattatcta gctccttagc tgcttggatt gcaagcctga
    27601 tatgcggata ccctaatatc gcttcttcat atgcaccatt tacgccatat ttttgcttca
    27661 ccctatgacc atgagtggtt tttttatgac gatattttgt atcaggaaaa gcagcaagct
    27721 tgctgacatc atcaagtatt ttcgataaag aaaaaggctg ttccatactg attttttgtg
    27781 caatgacact gatcaatagc cccattgccc aaatcgctcc tttatgggta ttgatgccat
    27841 ttgtagcaat cagcattgcc ttttccgctt ttcgcccaat ctcagcaatt ctttctctta
    27901 actcctgatc aatagcatga tgccatgcta tttgagccat ttcttcaaaa aaaggagtta
    27961 gcgtcttagc agacttaaga aataaagcat agtccatatc ggaatgtgca cccgcatcca
    28021 gtgcatccac taatccaggt tttggactta atgttacttc tgcgatcaat gcttcctcta
    28081 ccaacgcagc gagttgctga gtcactgctt tttttcgttc gtcatttatc atgacactca
    28141 ctccttcttc ctactattac ttgctgaaac cttttttgat agctacccaa atgatatgaa
    28201 gtaaccagct taaagcaaat gcacaacttg ccgtcattag ataagctaca agaatcatga
    28261 ctcctgaagg taactgcagc caactatctt taaacaataa taacagtact tggaaaacaa
    28321 atacattggc taaatacgta cgataagcat aaacagacaa ccatttgaac aatggcaatt
    28381 ttggcgaatc gtttacaatc attttggaag cgcctatgaa gacagcaaag atgatgacta
    28441 aactgtataa actttggatc gtattcaagt aaggtgcatt cgcaaagctc aagtctcctg
    28501 gatgtgcgag caatgaacgg acagaaaaga acgcgataac taaggcaaca ggtaagaaaa
    28561 gataacgtac tctgtccaaa tattgataga ctttttcgtg ataaacaaat gcggctaccc
    28621 cataaatcat gaaactagca acaaaccgat ctaataagta ccagctgtca tgataaggcc
    28681 cagtcaatac ttgtgtttga tagaacagat accatgcgac atataaaatc gttgaaccaa
    28741 tcaatactgg ccatacatat tttttattag caaatacttt gtaaccgagc caaacaaaaa
    28801 acggcatgaa caattggatc tgcagcatca taacggtata ccataaatga ggcgctccat
    28861 cacctgtgac atatttcagt aaaaagcttg cagtatccgt atacggagtt tgttgttgga
    28921 ctgctggaaa tagccaaaga taagccgtcg tccatagaag atacggaacg actagttcaa
    28981 aaaacttttc tttcaaaaat tcccaataag acaaatgctg acttgttttt accatattat
    29041 aaacaattgc aaaaataaac attggtgctg tgtatttaac gatgacgtaa aaggtaccaa
    29101 gaaaagctgt atttgttgcg ctgtggctgt ttgccaaagc aaagctcaat atcgtttgca
    29161 tcataacagc cgttacagct gatactttct ggaagtcact tacgttgatt ttgatttttt
    29221 tgttttcaaa catctctttc tctctctttt tctttattac caaagttctt tggttaattt
    29281 aggtccttcg actgttttca atagaatttt tttctctggc tctctcaaat actcttttag
    29341 tgaaaaaccg ccttgccgag tcactacttg ggtatcgaat tgctcaaaaa aagcaggatg
    29401 cgattgaatt acctgaattg gcaattcaat cggtgaattt gcgtacagca gcaaatcaaa
    29461 atcactggtt tcttttactg ccggaatccc agtcgataat tcaaatccta agctgccgcc
    29521 gattccccat ttacatcctg ataatatgtt tctagcagca tgaaattgct gataaacagg
    29581 ataatccatg attttctctt gtctatacga ttcttgttcg atgatgtccc acggatgaat
    29641 gacttgtttg acggcatgtt ttgagatttc tgttgaccat ctctgattgc gggcaggtcc
    29701 acgtaaacca atcggtagta tttctgcgtg atccgtcgac gcacgacgaa ctgttccgaa
    29761 catcttttca gttagaacga cccattcagg caaggtgaac ccttctattt catgtttatc
    29821 aaaaaggaca atatcatgtg cttgaatcat tttttatgca tcccattctt cgtccatttt
    29881 ggcacggact ttatttgttt cgacacgtcc gaatgttttg gctgtttcgt ttgtgtatcg
    29941 atttgtcagc atcgtgtcat ttgtattgcg gacatcttga atggcttcat tgattgcttc
    30001 gatgactttg ctgacatttt gttctgaagc agcttggtcg gtgatatctt ctaggaaatg
    30061 atagatcgct cctagttttg tataattatt gatatcgtaa gcgatagctg gaactttttc
    30121 agcagctgct tcgatttcag caaccgaacg ttttgtcaca cgtgcagcac tttctttaga
    30181 catcgcttgg acggtaattt ctttattgtt taaagcaatc aagcggcttg attgtaatcc
    30241 atgtgctaaa aatgcacctg aaatagcatt tcctgcaatc aatccaataa ctggatgtcc
    30301 agcttgacgg agttttgcat aagcagcagc actattagcc aaagccacat ggataccgat
    30361 caattcttcg ttatagccgt atgcttggct tggtacatcg atcacaacga tgattggccg
    30421 tttcactttt ttgtctttat cttcttcgta aacgtgatcc aaaatttgtc ccatgcggaa
    30481 accttcaact aaaccaactt caccgtttcg tacacggtac atcgggtttt taggatcagg
    30541 tacgattgat gtcacgatgg cttcttctat gaatttactt gatttaccat aatacacagt
    30601 actaatagaa gaagttggat ttttcatgcc agttagtgcc gcaaaccaat cgtaaccaac
    30661 acttgctgca gctggttcat ttccttctgc tacttctggt aagtcaattg attttgcttg
    30721 atggttagca attgcttcat tgtaatcgtc aatggccatt ggttttgaaa gatctaaaga
    30781 atcaagtaat gataaataaa atgctgagct ttcactgcga tgcgtgtctt ttcgttcgtc
    30841 cattgctgcg atgacagcag ctttgattgc ttctacacta tcagtcacta attcatcaat
    30901 gatttttgat tctacccgtt gacgagatcc taatgtattc caaatcaagt ctttgtcact
    30961 agaatcaaat tctcgaacgc ctgcttcttg ttcaataact tctggtccat tcaaaccgac
    31021 tctcgctttt tttgttgcga ttaaataact cattaatgct gaagtaatcg acataccgcc
    31081 aaatgatcct acacgtcctg gaatcaaacc gatgactggt acatattgtt ttagagcaat
    31141 gattacattt ccgatttctg aaatagataa cagtccgtag tttgcttctt gcaaacgaac
    31201 accacctgta tctagtacaa tgactggaaa gatctcattt ccttttttat tttcttctaa
    31261 cacatgttca agtgcagcaa cgatttttgc accggaaact tcaccgatac cgccaccttg
    31321 gaattttcct tccatagcga ttactactgc ttgtttgcca ttgatcgtgc cacggctgac
    31381 aacgatcccg tcatcacttt cagggacgat tccttgtgct ggtaaacttg gcgcgatgat
    31441 ttgatcgaat ggatcaatca attcacgtcc attgtcaagt agagcgttca ctcgttctct
    31501 ggcgtttaat tctacaaaac tatttttcat ctgaaagttc ctccattgct tgcgtcaatc
    31561 gtaggtacac aactcctggt gtagcaccga agtcattgat cacgatatct gctgaaattg
    31621 gataacgatc aaaaaatctc gttaatacat tgttccatac ttcgccgaag ccatcgcttc
    31681 ctgtacaaat ttttacagta gattctgttt tttctgttgg aaacatcaag atttccaaat
    31741 ctcctgaacc gaccactcct acatggattt tcttattgat cggctgtttc gttggatatt
    31801 tataagctaa ttgttccatg gatctctccc ctttttgtaa aacagagcat acacgttctg
    31861 cttattcgtc tttcttcttc catctgggta aatggcgaca aaaggttttt ctcgccaatt
    31921 ttatgaccaa gttctgaatt tcattggtgg ttcatacagg ccatctgacc attcgatcaa
    31981 atcatcgatg gtttgtgcag ctaataatga acgtttcgct tcactacgtt tgatgcctag
    32041 atcttctggt aatttcacga tgccggctgc acgcaagcta tctagtgatt ctttcgtgct
    32101 agtcattcca agaggtgtaa caccgccaat tgctgcaatt gctgcttgtc gttcttcttt
    32161 tgtttttgct ttgtacaaat aagcaattcc ttcttctgtc acgatatgtg tagtatcttc
    32221 gctataaatc atcactggtg cattggctaa gcctgcttgt ttttgaacag ctagtgcatc
    32281 taattcttcg acaaagactg gttttttatt tgaaccatat gtttcaacca tttgaacaac
    32341 taatttttgg cctttcccta atggatcatc attgtcgcga agtgattgcc atgctggtgt
    32401 agaatgacga cgaccgccag gattatgtcc catgttaggc gcaccgccaa aaccagataa
    32461 acgtcctttt gttacggtag aagagtttcc ttcgtaatct aattgcaagg ttgaaccgat
    32521 aaacatatcg atggcatatt gtcctgcagc ttgtgataat gttcggttac tgcgcatcgt
    32581 gccgtcttta ccgacaaaaa agacatctgg gcgagcagca atatattttt ccataccaac
    32641 ttcgccacca aaactatgga tgctttcaac ccaacctgat tcgatggctg gaatcatcgt
    32701 tggatgtggg ttcaataccc agttcttcgc gattttccct tttaacccta gtgattcacc
    32761 ataagttggc agtaacaatt cgatagctgc tgtattgaat ccaataccgt gattcaaaga
    32821 ttgtacgccg tgttctgcgt agattccttt gattgccatc attgccatta gaatttgcaa
    32881 ttctgtgatg ttttgaggat ctcgtgtgaa taaagcttct aattgataag gtttatctgc
    32941 tttcacgaca acgtctaccc agtcaccagg gatatctaca cgtggtaaag aatcgacaac
    33001 ttcgttgact tgaacgatca cgataccatc tttgaaagct gcagcttcga tgattgttgg
    33061 tgtttcttcc gtattgaaac ctgtgtacaa gttgccgtct ttatctgctt tatctgcagc
    33121 tactaaaaca acatttggaa tcaaatcgat gaataagcgt ccataaagtt ccaaataagt
    33181 atggatatca cctaatttca ttgtgccgtc ttcaatcatt tgggcgacac gcaagctttg
    33241 tgcaccagca taagagaaat cgatcttgct tgcgattcct ttttcaaaaa tattcaaatg
    33301 ttctggtcgt gagatactag acatgatcaa atgcagatca ttcacttttt ctgcatctac
    33361 tttttcaaga ctttctgaaa gaaaacttgc ttgtttttga ttgtcgcctt ctaatacaac
    33421 tttgtcgccg ggagcaatca gtttttccat tacttctacg attttttcag tttctacata
    33481 cttgccattg agcaagttgc tgattttttc caatcgctgc gctttttttt cgcgtttaga
    33541 agcccagttg cggtttggtt tttccataat tagtcatctc catttttgat atagaaacgt
    33601 ggcgtcatca ataccatgtt ggtctagatt gatcgtacgg atccctttat ctacaccaaa
    33661 ttttgtatga atcagctttg tcaaagtatt gccgggggga aattcaatcg tcagatccat
    33721 tcctaattct tttgccaagt ccatcatttg gttccactgc acagggaaag ctaggttttc
    33781 caacaaatct ttacgaatct cttctgcgtc ctgagtggct cttccatccg tattttttat
    33841 atacaaacat ctagcatcag atagatggac agactgcata tacggcttga atttcttgac
    33901 cgtatcattc atcaagagac agtgagatgg cacagggact ttcagcagct tcgctgtttg
    33961 cgcttggtgc cgtttggctt ctgccaacgt aacggataaa ccttcgatac tcccagataa
    34021 cgtatgttgc atcggacaat tttcatttga cagataaact ggatggtcag gatcaaatgt
    34081 ttttgctaca atttcctcag cttcgcttct cgtcacacct actactacgc ccattgcata
    34141 accgcttgga taagcttctt tcattaagac tgcgcggtga tagactagtt ccgctgcatt
    34201 ctcaaatctc aaagatcgac aagcaacagc tgcacaaaaa gcaccaatcg aatgtcctgc
    34261 tgcaatatca ggctgaatac cgtttttttc catttcatct aaataaaata ctgcttttgt
    34321 cagtaatgct agctggataa aaaccgtatc ttgatagttt tcttctttat caactaaccg
    34381 ataccccgtc acattttcta aacgttccat gtacatttca gggatatccg ctaacatccc
    34441 taacttctga ctgccttgtc caggaaataa gaaacctgta ctcatgagcc tcgcctcctc
    34501 agcacttact ttttccactg ctatcttagt caatttctgc tataataaaa aatgccaaaa
    34561 aaagaaaaaa ccataacaaa aaggtatgga tgggatggat aattatgaaa ttgcttcatc
    34621 tgcagtattt taaaaaagta ttcgaaacaa aaaatgttac ccaagcagcc aaagaattat
    34681 ttatttctca acctgccttg agccgtgcga tcaaacattt ggaaaacgaa ttaggtgtcc
    34741 ctttgtttta ccatagcggc agaaacattg agacgaccgt ttatgcagaa gaattttatc
    34801 catatgcagt caagacatta gacacattag aagaaggaat caaattattg tcttcggtca
    34861 atgagaagat cgttacttcc gtcatattgt atttagaggt agcctctgtt tctattccga
    34921 acttggtccg aatttttatg gaaaagcatc ctgatatcca attaacgatc atgcaacatc
    34981 atttaccaga tgatattacc gatcctgtac tgtacatcac ttcggagatg aagcctggca
    35041 tgaccaatat tcctatcatg aaagaacctg tttatgtcgc cattcctaag aaacatcctc
    35101 ttgctaaaaa agaacatctg caattagaag atatccaacg cacccccatc ttgatgctgt
    35161 caaagagcaa cgccttccgt catacactgg atgcagcact tgaagcgaaa agtatcgaat
    35221 tgacgatcag ttcgacgaca gatgatcctg cgacattacg ttctattgtc aagcaaggat
    35281 tagctgttag ctttttccca aaagtttctt ggtcttacga taaaaatgat cctttcgtct
    35341 tgcgggaaat caaagatttg cccttgacac gaacaattta tttatcttct agcttcacag
    35401 aagatcatcc tttgatcaaa atgattgcaa aaacgatgcg ggaattttat ttaggaaaat
    35461 aaaaaaaaaa gagctgggac aaaattc
//
LOCUS       DAZULY010000004        33432 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000004 DAZULY010000000
VERSION     DAZULY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.004"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            74..505
                     /gene="mraZ"
                     /locus_tag="ACG81A_000110"
     CDS             74..505
                     /gene="mraZ"
                     /locus_tag="ACG81A_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFS5013521.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            526..1485
                     /gene="rsmH"
                     /locus_tag="ACG81A_000111"
     CDS             526..1485
                     /gene="rsmH"
                     /locus_tag="ACG81A_000111"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFS5013522.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            1490..1891
                     /gene="ftsL"
                     /locus_tag="ACG81A_000112"
     CDS             1490..1891
                     /gene="ftsL"
                     /locus_tag="ACG81A_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFS5013523.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            1892..4084
                     /locus_tag="ACG81A_000113"
     CDS             1892..4084
                     /locus_tag="ACG81A_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS5013524.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4110..5072
                     /gene="mraY"
                     /locus_tag="ACG81A_000114"
     CDS             4110..5072
                     /gene="mraY"
                     /locus_tag="ACG81A_000114"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFS5013525.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5086..6447
                     /gene="murD"
                     /locus_tag="ACG81A_000115"
     CDS             5086..6447
                     /gene="murD"
                     /locus_tag="ACG81A_000115"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFS5013526.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            6473..7561
                     /gene="murG"
                     /locus_tag="ACG81A_000116"
     CDS             6473..7561
                     /gene="murG"
                     /locus_tag="ACG81A_000116"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFS5013527.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            7576..8796
                     /locus_tag="ACG81A_000117"
     CDS             7576..8796
                     /locus_tag="ACG81A_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFS5013528.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            8952..10277
                     /gene="ftsA"
                     /locus_tag="ACG81A_000118"
     CDS             8952..10277
                     /gene="ftsA"
                     /locus_tag="ACG81A_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFS5013529.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            10300..11541
                     /gene="ftsZ"
                     /locus_tag="ACG81A_000119"
     CDS             10300..11541
                     /gene="ftsZ"
                     /locus_tag="ACG81A_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFS5013530.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            11558..12235
                     /locus_tag="ACG81A_000120"
     CDS             11558..12235
                     /locus_tag="ACG81A_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFS5013531.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            12249..12848
                     /locus_tag="ACG81A_000121"
     CDS             12249..12848
                     /locus_tag="ACG81A_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFS5013532.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            12929..13150
                     /locus_tag="ACG81A_000122"
     CDS             12929..13150
                     /locus_tag="ACG81A_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFS5013533.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            13199..13981
                     /locus_tag="ACG81A_000123"
     CDS             13199..13981
                     /locus_tag="ACG81A_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFS5013534.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            14002..14709
                     /locus_tag="ACG81A_000124"
     CDS             14002..14709
                     /locus_tag="ACG81A_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFS5013535.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            14971..17769
                     /gene="ileS"
                     /locus_tag="ACG81A_000125"
     CDS             14971..17769
                     /gene="ileS"
                     /locus_tag="ACG81A_000125"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFS5013536.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(17818..19344)
                     /gene="zwf"
                     /locus_tag="ACG81A_000126"
     CDS             complement(17818..19344)
                     /gene="zwf"
                     /locus_tag="ACG81A_000126"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFS5013537.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(19359..20006)
                     /locus_tag="ACG81A_000127"
     CDS             complement(19359..20006)
                     /locus_tag="ACG81A_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS5013538.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(20190..20519)
                     /locus_tag="ACG81A_000128"
     CDS             complement(20190..20519)
                     /locus_tag="ACG81A_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFS5013539.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            20696..21424
                     /gene="sfsA"
                     /locus_tag="ACG81A_000129"
     CDS             20696..21424
                     /gene="sfsA"
                     /locus_tag="ACG81A_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFS5013540.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            21440..22453
                     /locus_tag="ACG81A_000130"
     CDS             21440..22453
                     /locus_tag="ACG81A_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5013541.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            22453..23730
                     /locus_tag="ACG81A_000131"
     CDS             22453..23730
                     /locus_tag="ACG81A_000131"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS5013542.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            23793..26495
                     /locus_tag="ACG81A_000132"
     CDS             23793..26495
                     /locus_tag="ACG81A_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5013543.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            26647..26964
                     /locus_tag="ACG81A_000133"
     CDS             26647..26964
                     /locus_tag="ACG81A_000133"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5013544.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            26994..27314
                     /locus_tag="ACG81A_000134"
     CDS             26994..27314
                     /locus_tag="ACG81A_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS5013545.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            27422..28882
                     /locus_tag="ACG81A_000135"
     CDS             27422..28882
                     /locus_tag="ACG81A_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5013546.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            28950..29171
                     /locus_tag="ACG81A_000136"
     CDS             28950..29171
                     /locus_tag="ACG81A_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013547.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            29202..29384
                     /locus_tag="ACG81A_000137"
     CDS             29202..29384
                     /locus_tag="ACG81A_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFS5013548.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            29384..29797
                     /locus_tag="ACG81A_000138"
     CDS             29384..29797
                     /locus_tag="ACG81A_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFS5013549.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            29920..31101
                     /locus_tag="ACG81A_000139"
     CDS             29920..31101
                     /locus_tag="ACG81A_000139"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5013550.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(31172..31336)
                     /locus_tag="ACG81A_000140"
     CDS             complement(31172..31336)
                     /locus_tag="ACG81A_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013551.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            31490..31563
                     /locus_tag="ACG81A_000141"
     tRNA            31490..31563
                     /locus_tag="ACG81A_000141"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31524..31526,aa:Arg,seq:cct)
     gene            complement(31609..31722)
                     /locus_tag="ACG81A_000142"
                     /pseudo
     CDS             complement(31609..31722)
                     /locus_tag="ACG81A_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(32105..33337)
                     /locus_tag="ACG81A_000143"
     CDS             complement(32105..33337)
                     /locus_tag="ACG81A_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS5013552.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT        11128 a         5807 c         7094 g         9403 t
ORIGIN      
        1 cccagaaaag gtggtagaaa gtgggggatt gtggtagact aattttagtt agtggaggaa
       61 tggggggctt tcaatgttga tgggtgaatt ccaacataat attgatgcaa aaggacgttt
      121 aatcgtgccc tcaaaacttc gtgaagaact tggcgaaaag tttgttctga cacgcggatt
      181 ggatggctgt cttttcggct atccgatgag cgaatgggaa aatctggaag ctaaactgaa
      241 cgaaatgccg ttggctaaaa aagacgcacg tacttttgtg cgattcttct attcggctgc
      301 cactgaatgc gaacttgata aacaaggacg aatcaatata cccagcacgt taagaaatta
      361 tgcagctttg acaaaaggtt gcgtagtcat cggtgtatct aaccgaattg aaatttggga
      421 tgagacacga tggcaagatt tttctgcagc agcagaagag aactttgacg aaattgctga
      481 aagtatgatt gatttcggac tataagaata ggaagaggct gactaatgac tgaaacattt
      541 caacactata cagtcatgtt gaaagaaacg gtagatggat tgaacatcaa accggatggc
      601 gtttatgtag actgtacact cggcggtgcc ggacatagtg aatatttatt aagccaacta
      661 agttctaaag ggcatctgta tgcgtttgac caagatcaaa aagcaatcga tcacgccaag
      721 ctgcgtttag cttcttatgt cgaacaaggt atggtcacat ttatcaaagc gaacttcaga
      781 gaattagaag aacgcttgag tgagcatgta gataaggtag acggcatttt gtatgatcta
      841 ggggtttcct ctcctcagct agatgaagca gaacgtggat ttagttatca tcaagatgca
      901 ccgttggata tgcgcatgga tcagtcagct cctttatccg cttatcacgt aattaatgaa
      961 tattcctacc atgaattagt aaagattttc ttccgctatg gcgaggagaa attttccaaa
     1021 caaattgctc gtgaaatcga acgagtacga aaaacaaaac caatccaaac aactggtgag
     1081 cttgtcgaaa tcattaaaca agtcatccca gctcccgcaa gaagaaaagg cgggcatcct
     1141 gcaaaaagaa tttttcaagc tgtgcggatt gcagtaaatg acgaactcgg tgccgaagag
     1201 gcctctttag aacaggcaat ccggttattg aaagtcaatg gaagaatcag cgtaatcaca
     1261 ttccattcat tggaagacag aattgtcaaa agtatgttta aagaatatag tacggtacaa
     1321 gatctaccac cgggacttcc tgtcatacct gacgaattcc aaccagaact gaaagtcatt
     1381 actcgtaaac caatccttcc ttcagaggaa gagctaacag aaaacaatcg atcacgcagt
     1441 gcaaagttac gtattgcaga aaaaataaga atgaaagagg agtagaatga tggcagaatt
     1501 aaagaaaatg caagattacg aatatgattt acctgaattg gtggatcagc cggaacaaca
     1561 aatgcctgat tcttcttcga atcaagaaat cattcttcca caatcaccgg caaaacgatt
     1621 aaagcagatt tctgctttgg aaaaaattgc tgtggcaagc gtggcgattg ctattgttgc
     1681 tttatgtgtt ttaacagtta tgctgcgcac aaatatcagc ggagctgaaa aagaaatcac
     1741 gtcaatacaa acagaaatca atgataaaaa gcaagaaaaa acgagtttag aacaagaaaa
     1801 aaacgaactt tctcgtactg aacggattaa acaaatcgca gaagaaaaag gattgtctat
     1861 taatgacgac aatttaagga aagtgaagta aatgagtctt aaaaataaaa ttcggcggtt
     1921 tatggaaaag aaaaatctaa atccgatgaa caatcgcaaa aaagtaggta tcattttatt
     1981 tgctacgagt attggattgt tctttttatt tgccgtccgt ttctcgtata tcgttattgg
     2041 aggtcatgtt gctggtacct cattagctga aaaaacaaaa cagttgtacc aaggaagtga
     2101 agtagtaaaa gcaaaaagag gaacgattta tgatcgtaac ggtgtggcgc tcgcagaaga
     2161 tgcctcctct tattcaatta aagcgatact ctcaaaaaca tatacctctg gggataaaaa
     2221 actgtatgtt gaagaaaaga attttgataa aatagcagaa atcctgcata agaatctttc
     2281 tatagacaaa aaagatgcac tgaatatttt agaggacggc gcaaaaaaag agctgtatca
     2341 agttgaattt ggtagctatg ggaaaaatat ctcccaagaa accaagcaaa acatcgaagc
     2401 ggacatgaaa aaagaaggtg tcgctggtct ttattttgtc gaccatcagg ctcggatgta
     2461 tccaaatggc gtcttttctt ctcattttat tggatacgca gttccagaca aagatgagaa
     2521 tggcttagtt ggtaaactag gtttggagtc tgcttacaat gatatcttga gcggtaaaga
     2581 tggtaagatt atttatcaaa aagataattt tcaaaatcct ctgccaggta cagtggcaga
     2641 agaagaaaaa gctgtcgacg gacaagatat ctacacgact ttagacagca gactacaaag
     2701 ttatttagaa acactgatgg atcaagtaaa tgaagaatat cagccagaag aattgacagc
     2761 agtcctgatg aaagcaaaaa ccggagaaat cttggcaatg ggacaacgac ctactttcaa
     2821 tcctgaaaca atggaaggat tgactggaaa agatgctatc tggcgtaatt tcttggtaca
     2881 agatagttat gaaccaggat caaccatgaa agttttcact actgcagctg ctatcgaaga
     2941 aggagaattc aacgagaatg aaaccttcca atctggaaaa atccaagtag aagatgctac
     3001 gatcaatgac catgactttg gtgaaaaagg tgtgctgacc atgcgtcaag ctctttcatg
     3061 gtcaagtaac gtcgggatgg tcatactgga acagcgatta ggcggacgat ggtataacta
     3121 tttacaaaaa ctgggatttg gacaaagtac tcattctgga ttagatgatg aagtaaacgg
     3181 tgctttacct acattaaata ttgtcgaccg agcaatgagt gcttatggac aagcggtagg
     3241 tgtgacgaat ttccaaatga tgaaagcttt cacttctatt gcgaataacg gtacaatgat
     3301 tcaacctcga tatatcagca aagtggtgga tccccaaact ggagaagagc gaacaactca
     3361 aacagaagtt ctagggcaac ctttttcaaa agaaacaaca gaaaaagtcc gtgaatatat
     3421 gagagatgtt gtcgaaagcg aaaactacgg tagtgcttat ggtgtatata gtgtacctgg
     3481 atacaatgta tcggcaaaaa acggaacagc ccaaatcgct tctgatacag ggggatatca
     3541 aacaggagat acagcctacc tgtattcgat tgtggagatg gttccttctg aagatccaga
     3601 ctatgtcttg tatctaacaa tgaaacaccc taagacatat gatcgaatgg cattggctaa
     3661 aatcgcaaat ccattgatga aacgggcaat ggattttaaa gaaactgaag aagattcaga
     3721 tacagaaaca aaaacagaaa aagtatccgt agccgattat cgtaatcttg aagcggacgt
     3781 tgcagcagct gatgctcaaa aaagtggact gcaacctgta gtgatcggta atggtaaaaa
     3841 agtacaaaaa caatctactg ccaacggtga tcagttgatc tctggagaaa aactgatcct
     3901 atatactggt ggtgataagc tgatgcctga cgtaacagga tggtcaaaag cagatatcat
     3961 gaagctgggg aaaatcctcg gtattgaagt tagttttgat ggagatggtt attgtgtcaa
     4021 acaagaactt gctccatatg aaaaaatcac tgaagacaaa cttagtttta ctttagaaga
     4081 ataagaagag aaataggaga gaaaagagca tggaatggac acaagcattg attcccattg
     4141 tcagcagttg tgcaatgaca attgccgcaa tgccgctatt tattggttat tttcaaatga
     4201 aaaagcaagg tcaggcgatt cgtgaggagg gaccaaagtg gcacaatagc aaagccggaa
     4261 ctcctacaat gggcggctta gtgtttctga tcggctctat tttgacagga atttgggttg
     4321 gagcttggca aaaacagcta actcctactc ttttcatctt gctttttgta ttagcgttat
     4381 acggagtaat cggctttctt gatgatttta taaaaatatt caagaaaaga aacatgggac
     4441 tgaactcgaa gcagaaattg attggacaga ttctaggggg aatcatcttt tatcttgttt
     4501 accgttctga aggttatcca ggtgtattga atttcttcgg actagatcta cctttaggaa
     4561 tcatttatgg tattttcgct ctcttctggc ttgttggatt ttcaaatgca gtaaatctga
     4621 cagatggcat cgatggatta gttgctggct taggaagtat ctcttttgca gcttacggtc
     4681 taattgcttg gcatcagcat cagtatgatg tattaatcat ttgcttaagt gttttaggag
     4741 gtttactggg tttcttcgtc tacaacagaa aacctgccaa gatttttatg ggagatgtcg
     4801 gttcattagc gttgggcgga cttttagcgg caatttctat aatgctaaac caagaatgga
     4861 cattgctttt gattggtttg atctatgtta tggaaacagc aagtgtgatg cttcaagtta
     4921 cttcctttaa gcttacagga aaacgaattt ttaaaatgtc tccaattcat catcactttg
     4981 aaatgtgcgg atggtcggag tggaagattg atacagtttt ttggttgatc agcattgtga
     5041 cttcgttgat cacattatgg atcgtttggt aggagatgac gggaaatgaa aaaaatatca
     5101 acatatgaaa ataagaaagt gcttgtctta ggactcgcaa aaagcggagt aagtgcggcc
     5161 aaattattac atgaattggg cgcacttgtc accgttaatg atggaaaacc ttttgacgaa
     5221 aatccggaag cacaagagct gctttcttta gggataaaag taataacagg tagtcatcca
     5281 atcgaattgt tggatgaaga attttcattg atggtcaaaa atccagggat tccctacagt
     5341 catcctttag tagcaaaagc ccaagaaatg ggtattccgg taattacgga agttgagcta
     5401 gcttacgaag tagcggaatg cccaattatt gggattacag gaacgaacgg aaaaacaacg
     5461 acaacaacga tgacaggact actactgaat gcaggagcag atcaaggaat tgcacgttta
     5521 gcgggtaata ttgggtatcc agcaagtggt gtagcgcaag aagcaaaatc agaagataaa
     5581 attgtgatgg aattgtctag cttccaactc atgggaatca cagatttccg ccctcatata
     5641 gcggtgatta ccaatatcta tgaagcacat attgattatc atggtacaag aaaagaatat
     5701 gtcaaagcta aatggaacct tcaaaaaaac atgacagaaa aagattatct gatccttaac
     5761 tggaatcagt cagaactaca agaattagcg caaagaacaa aagcacgcgt actgcctttc
     5821 tcgacaaaag aagtgttgga agacggtgtg tatgctgacg attactcaat atactataaa
     5881 aaagaaaaaa taatggaaat atctgaactt ggcgtaccag gtaagcataa tgtagaaaat
     5941 gcactagctg ctatttctgt agcaaaattg tatggtatat caaatgaagc tatacgagaa
     6001 acgttgcatt ttttccatgg cgtaccacat cgtacgcaat atgtaggaga aatccaaggc
     6061 agaaaatttt ataatgattc aaaagcaacc aatattttag cgactaaaat ggctctaagt
     6121 ggatttgaaa cgtctaaggt cgtgttattg gctggcggat tagacagagg aaatacattt
     6181 gatgaattaa ttccttcatt aaaaggaatc aaagcaatgg tcgtatttgg ccaaacaaaa
     6241 gaaaagttga tggatgctgg aaaaaaagca ggcatcgaga caatagtaac agccgattct
     6301 gtagaacaag cagttccttt agcattagaa aacagtacag atggtgatgt agttttatta
     6361 tcaccagcga atgccagctg ggatcaatac ccaaattttg aaactagagg aaacagattc
     6421 atggaagctg tgaaccgatt aaaatagaaa aagtagaagc gagcgataaa aaatgaaaat
     6481 tttggttaca ggcggaggaa cagggggaca tatttatcca gctcttgcct ttgtcaatta
     6541 tgtaaagact caagaaccga atgctgaatt catgtatgtg ggagcaaaac gaggtctaga
     6601 aaacaaaatc gtccctgata caggaatgcc tttccatacc ttggagattc aaggatttaa
     6661 acgaaaaatt tccatgcata acatcaagac ggtccgattg tttcttaaga gtatccgtca
     6721 agcaaaaaaa atcctcagtg attttcaacc agatatcgtt atcggtactg gaggatatgt
     6781 ttccggagcg gtagtgtacg ctgcagccaa aatgggtatt ccaacagtta tccatgaaca
     6841 aaacagtgta ccaggtgtta ccaataaatt tttaagccga tatgtggatc ggatcgctct
     6901 ttcatttaaa gatgcggctt ccttttttcc tgaaaacaaa gcagttcttg taggtaatcc
     6961 tcgagcacaa gaagtagcag atactaaaaa atcagatatt ttgaaaacat ttgatttgga
     7021 tccagaaaag aaaacagtct tgatttttgg cggtagtcaa ggagcactta aaatcaatca
     7081 agcagtatca gtttttttaa gtgaatatat tccttcttct taccaagttt tgtatgcttc
     7141 aggagataga tattatgaag agatcctaaa taaaataaat cctgaaatac aaaatattag
     7201 tgtccagcct tatatcaaaa atatggcaga ggtcatggct tccagtgatc tgctggtggg
     7261 gcgtgctggt gcaacttcga tcgcagaatt cacagctctg gggttaccgg cagtgttggt
     7321 tcccagccca tatgttacga atgatcatca gactaaaaat gcaatgagtc ttgttcacgc
     7381 aggagcagca aaaatgattg cagataatga actaacaggt gaatcactaa gtcagacagt
     7441 caacgaaatc atgggtgatg aagaattgca aaagcaaatg tgccgagctt ctaaagaaca
     7501 aggaatccct gatgcttcaa aacgtcttta tgacttagta aaacaaataa tcaaaaaata
     7561 aaggcggtgg tcttagtgag taagaaaaaa gaaccaaaaa aattgtctca tccgtcttct
     7621 gatgaaaaca atttgactcc ttggcaaaag gctaaccgaa aatatctggc agaacaagag
     7681 atgaaaaacc aagagccaaa aaaagaagac ataacattag atacacccct ttcagattct
     7741 gaagaagaaa aaacggttca agaggaacag caagaagaaa gccagtccga gaaaaaaatc
     7801 gaattttttg aagagatcaa gcaaaagaaa aacggtggac catataacgg ttcttttttg
     7861 aaccggctgc ccaatttaaa aagtcaacgg cacaaagtac tatttcgtcg gttagctttg
     7921 atcatcactg tattgggtat accgctgatt tttttgatct attatgtttc tccttatagc
     7981 aaattacaag cagttgcagt atcaggcaat aagacagtca acagccaaga aatcatctct
     8041 gatacaaaat tgtcattagg agaaaatgtt tggggacagt attttcatcg atctacttat
     8101 atcgatcgcc ttaaaaaagc acagccaaga atcgaaacag caaatataca tttcaaaggg
     8161 atgaatgaat tcgatttgga tgtaacagaa tataaagaga ttgctttgat cgcaaaaaac
     8221 aatcaatact acccagtgat tgaaaatgga acagtattag atgaaaaagt agcaaatcct
     8281 acaaaaaatc tccctatctt ggaagagttc aaagactctg caaaaatcaa agaattaaca
     8341 aagcaataca atcagttatc atcagaatta caaaaagcaa tttctgaaat caagtacacg
     8401 ccaagagcaa gcaataaaaa tctgatccaa ttgaatatga atgacggcaa tcaagttatt
     8461 gtaaatatca ataatcttgc gaaccaaatg aaatattatt cgcaagtagc caaagatatg
     8521 gatgaaaaag gtgtgatcga catggaagtt ggtatcttct cgcatcctta cggtagcact
     8581 caagaaacgg aagaatcggg ccaatcggaa gaaacaactg aaagtgaaga aaatgcggat
     8641 caaacaaacg aatcaagtga agaggcagaa acgcaaaaag cacaaacacc tggatcgtct
     8701 agcacaaacg gatcatctga ttcaaatgat tcaagtgaaa caagtcaaat agaagataat
     8761 ccaagtacaa ctccgtcaag tttaaatgaa aattgataaa acaactttaa aaagtaagaa
     8821 tgtactttct aaaaaccagt aatttatggt agaattggaa gcagtgaata tatctattaa
     8881 taagtatatt ttaaaacgga cgctagttgt tcgcttgtat atggaatagt aagtaggagg
     8941 gaatcccatt catggcaaaa acaggaatgt atgtaggcct tgatattgga acgacatctg
     9001 ttaaagttgt cgtggctgaa tatatcgaca gccaaatgaa tattattgga gtaggaaacg
     9061 caaaatcaga agggattaac cgaggcatta tcgttgatat cgataaaacg gtccaagcga
     9121 tacaacgagc agtacgacaa gcagaagaaa aagcaggtat ccagatcaaa ggcgtaagtg
     9181 tcggtttgcc agcaaacatg cttgaagtag aaaattgcca gggaatgatt gcagttaacg
     9241 gagattcaaa ggaaatcacc gatgaagatg tcagaaatgt ggcttcagct gcccttgttc
     9301 gttcaatacc tcctgaacgt caaatcgtgt caatattgcc acaagatttt actgtcgatg
     9361 ggttcgaggg aatcaaagat cctcgtggaa tgatcggcgt aagattagaa atgtacggtc
     9421 tgttgtttac aggacctaaa acgatcatcc ataacatacg taaatgcgtg gaaaatgccg
     9481 gattaatcgt caacgaaatg gtgattactc ctttagcgct gactgaatca attctttctg
     9541 acggagaaaa agattttggt acgatcgtca ttgatatggg tggcggccaa acaacaacgg
     9601 ctgttatgca tgataagcaa ttgaagttta ccaatcttga ccaagaaggt ggcgaatttg
     9661 tcactaaaga tatctctatt gtattaaata cgtcatttaa caatgcagaa gcgttgaaaa
     9721 taaactacgg ggatgcatac ccagaacgga catcaccaga tgaagaattt ccagtagatg
     9781 tgattggtca gtcagaacca gtgaaagtcg atgaacgcta cttgtcagaa attatttcag
     9841 ctcgaatgga acagattttc aacaaagcaa aagatgcatt ggaccaaatc gaagcattag
     9901 agttgccagg tggcgtcgta ttgacaggcg gagctgctag tcttcctggt gttgtggact
     9961 tggcacaaga aatcttcggc gtaaatgtaa aattgtatgt tccaaatcac atggggctga
    10021 gaaacccggt atttacaaat gtaataagta tcgtggatta ttcagcaaac ttgagtgaag
    10081 tgtatcaatt ggcaaaaatt gctgttacgg gtgaaacctc agcagctcgt caaatggtag
    10141 ttgaacaaga aacaacaaac acatatgaga gctatgaagc acctgaagaa acaatctacg
    10201 atgagccgga accaaaagag tccggagaaa atgtaaaaaa taaaatcaag ggtttcttta
    10261 caaatatttt tgattaattc atcctgggag gaataagata tggaattctc aattgataat
    10321 aatatcaatg acggcgcagt aatcaaagtc atcggagtcg gtggtggcgg tggaaatgct
    10381 gtaaaccgaa tgattgaaga aaacgttaaa ggtgttgaat tcattacagc caacacagac
    10441 gtgcaagcat taaaaaattc aaaagctgaa acagttatcc aattaggccc caaatatact
    10501 cgtggtttag gtgctggttc tcagccagaa gttggacaaa aagcggcaga agaaagtgaa
    10561 caatccttac gcgaagcttt agacggtgca gatatgattt tcatcactgc aggaatgggt
    10621 ggaggaaccg gtacaggtgc tgcaccaatc gttgcaggta tcgcgaaaga attgggcgca
    10681 ttgactgttg gtgtcgtgac acgtccattc acttttgaag gaccgaaacg tggccgtttt
    10741 gctgcagaag gtatcgctcg tttgaaagaa aatgtggata cattattaat tatctcaaat
    10801 aaccgtttat tagaagttgt agataaaaag acaccgatgc ttgaagcttt ccgtgaagca
    10861 gataatgttt tacgacaagg tgtacaaggt atctctgatt tgatcacagc gccaggttac
    10921 gttaaccttg actttgctga cgtgaaaaca gtgatggaaa accaaggtac agcattgatg
    10981 gggatcggtg tggctagtgg agaagatcgc gtagtagaag caacgaaaaa agcaatctcc
    11041 tctccattgc ttgaaacatc tattgatgga gctgaacaag tacttcttaa catcacaggc
    11101 ggtttggata tgacattgtt cgaagcacaa gatgcttcag atatcgtagc aaatgcagca
    11161 actggcgatg taaatatcat tctaggtaca tctatcaatg aagaaatggg cgatgaaatc
    11221 cgtgtgacgg tcatcgcaac aggtatcgat gaatcaaaaa aggaacgcaa gtctagccga
    11281 ccagctagac aagcacaaat gcaatcacct gctcaaaaga ctgtattaga tatggatcaa
    11341 gctaaaccaa cttcttcaga agaagaaaac agttttggcg attgggatat cagacgtgaa
    11401 caaaatgttc gtcctagagt tgatgattcc aattttgaca atatcgaaaa aaaagaattt
    11461 gatacattca accgcgaaga aacaaaatct catggcgatg atgaattaaa tacgccaccg
    11521 tttttccgtc gtaaaaaata aaggagagga agagcacatg attgctgaca acttgcagaa
    11581 aatcaagcaa gaaattcagg attcgtgtgc tcttgttcat cgtgatccga aagaagttac
    11641 attagtagct gtaacaaaat ctgttgaatc cgctcaagct gaagaattgg ttagagctgg
    11701 cgttatgaat tgtgcagaaa accgagtaga taaattatta gaaaagaaac aagatttatt
    11761 tgattttcct gaaataaagt ggcatttaat tggtaattta cagcgcagaa aggtaaaatt
    11821 gattgtaaat gaaattgatt actttcatgc gttggacagt ttaagattag cagaagaaat
    11881 ccagaagcgg ttagagaaag aattatcctg ttttattgaa gtaaatgttt ctggtgagga
    11941 atcaaaacat ggaatttcac cagatgagct tttaccgttt gtggaatctt tagctgcctg
    12001 cacaaaaatc aaggttgttg ggttgatgac tatggcgcct aaagaagctt ctgatgagca
    12061 aatccataaa gtgtttggta cgctgaaaga attgcgtgat cttgttcaag caaaaggtta
    12121 tgcccatgca ccatgtagtg aattaagtat ggggatgagt caagactttc aaattgcgat
    12181 tgaagaaggt gcaacattta tccgtgtagg aacagcgcta ttcaaagacg aataaaggag
    12241 gaacaaaaat gccgctttta aacagaggag caattgctaa ttttttcgga ttagctgatg
    12301 aggaagagta cgaataccaa gattatcctg aaacaacaaa aaaggctgta cagccaacta
    12361 aaaaaaatac aacacagaat caatcaactc attctgtatc tgctgcgaat actcatgaaa
    12421 aacaaaaaac ggcgacttca cgtcctgtaa cgcatgaaag tattcagccg gctgaaaaga
    12481 aaaaagtaat tgaactacat caatcaccta caaaaactgt agcgaaagaa agaacagctt
    12541 caagaacctc tacacaacct aaagcaaatc ttgctggaaa aatcactgta atggaaccaa
    12601 gagcttattc tgaagctatg gcgattgcta aacgaatcat tgctggagaa gcaaccttag
    12661 tgaattttca tttagtagaa gaaaaacaag cgagaagaat cgttgatttt ctaacaggaa
    12721 cagtttatgc actagatgga gatatcaaac gtgttggaga tgagattttt ctttgtacac
    12781 cgactgggat ggagattgac agttcaaccg ctcaatcttt tgcagatgcg aatttgtttg
    12841 atctgtaaga ggaggaacta cgatttatac tattgcacta ttacttcatt tacttcaacg
    12901 ggcagcctat ttctatagca ttatcttgat ggtatatgca ttgctctctt ggtttccagg
    12961 cgggtatcaa tcagggctag gacgattatt agctaagatc tgcgaaccgt atatcagttt
    13021 gttcgatcgt ctgcctttga ggattggacc cttggatttc acaattttag gggcaatcct
    13081 gatcttgaac ttagccgtac aaggtttagc acagatcgtt atatggataa ctgtccattt
    13141 agtttattga ttgaagcaac aaaatcttta ttctcagaat agataggagg ggaaaatttt
    13201 ggatgcaaat gtataccagc actttcgggc agatgaacgt acatttatcg attcggtagg
    13261 cgattggatt gaacaggttc aaggacaata tgcaccctat ctgaccgaat ttcttgaccc
    13321 aagacaatcc tatattttag aaacactgat tcgacaaagc agtgatttgc gtttcatgtt
    13381 ttatggcgga tatgaacagg cagaaagaaa aagatgtttg attttccccg attattacga
    13441 acctgtagag gaagattttg aaacagcatt gtatgaaatc cattatccta gcaaatttgc
    13501 cagcctaagt catgggaaaa tccttggaac tttagtggga acggggatca aacgctctta
    13561 ctttggcgat atcatctcgg acggtgatcg ctggcaagta tttattgcaa atgaggtgga
    13621 tcattttgtt gctgcacaag taacaaagat tggaaaggtt gctgtacgct tggaaccaat
    13681 caaatacaca gaaatgatta tgccgaaaga tagctggaca caagaaagag gaacagttag
    13741 ttccatgcga ctagacagtg tgatctctga gatttataat atttccagac agcggtccaa
    13801 gcagctgatc gaagctggta aagtaaaagt gaactggaca gaaaatacaa gacctgattt
    13861 tgtacttgaa ctattagata ttatttcagt cagaggttat ggcagagtcc agatccaaga
    13921 acttgagggg aagacgaaaa aagaaaagta cagggtactg ttcggtgtat tacgtaaata
    13981 aaataaaaag gtggtttact catggcttta actccattag atatccaaaa taagacattc
    14041 ccgacaaaaa tgcggggata taatcaagac gaagtagatg atttcttaga tttagttgtt
    14101 cgtgactatg aggaattgac tcaaagaaat cgagaattag aaaaagcagt caaacattct
    14161 gaagaaaaac ttgagtactt caatgaatta aaagatgctt tgaaccaatc aatcatcgta
    14221 gctcaagata cggctgataa agtaaaaaca agtgcaagca aggaatccga agtgatcgtt
    14281 acttctgcac aaaacaaagc agatgagtta gttgcgaacg ctgaaaaacg tgcacatcaa
    14341 ttaacaactg atgcagaaga aaaagcaaga aaaatcttga cagatgcaac agaaaaagca
    14401 agacagcttg cgacagaaac tgaagatctt aagaaaaaaa cacgagtatt ccatcaacgt
    14461 atcagcttga tgcttgagtc tcaattagaa caagtgaaaa gtccggaatg ggatgaaatt
    14521 ttacaaccat tctccagcta cgtgactgac agccatgaag tgatcaaaga agttttagca
    14581 aaacagcttg acaatgaaaa tgatactgat gtaaactcag acacaggagt catagaagca
    14641 cctatgacgg catttgatac acaggctatt gatatctcga tcgtgcctgg tgaaaatgaa
    14701 aacgagtaga acagaaaagc actttgttat tcttaaagcg agccgatgag agtggaagtt
    14761 cggtaagaca ctgcatggtg aaagatcctc tacttgagac ctgcttttcc gaaattagag
    14821 taagaagcag cggctgacct cgttattggt cacagagagt ttgcactaga tagatgcaac
    14881 taaatttggg tggtaccacg gcatgttcgt cccttgcggc tgaacatgct tttttgtgtc
    14941 cggaaaacaa ataaaggagt gtatggttga atgaaaatga aagaaacctt gcaattagga
    15001 aaaacaaaat tccctatgcg tggtaatttg ccaaaccgag aaggcgagtg gcaaaaagaa
    15061 tgggaagaaa atcgaatcta ccaaaaaaga caagagttga acgaaggaaa acctagcttt
    15121 gttcttcatg atggacctcc atatgccaat ggtaatatcc atttaggaca ttcattgaat
    15181 aaaatcagta aagacattat cattcgttca aaatcaatgt ctggctatcg tgcaccatat
    15241 gttccaggat gggatacaca tggattgccg attgaacaag tattggctaa taaaggcgtg
    15301 aaacgtaaag aaatgtccat ggcagaatac ttggaaaaat gtcgtgaata tgcactttcc
    15361 caagtagata agcaacgagc agatttcaaa cgcctaggag ttgcaggaga ttgggaacat
    15421 ccttatgtta cattagatcc ttcttatgaa gctgcacaag tacgcgtatt tggtaaaatg
    15481 gctgaaaaag gctatattta caaaggattg aaaccaattt actggtctcc gtcaagtgag
    15541 tcttctttag cggaagctga aattgaatat aaagatgtaa aatctccatc tatttttgta
    15601 gctttcaatg tcaaagacgg caaagatatt cttgatgaag atacagcttt tgtcatttgg
    15661 acgacaacac catggacatt gccagcaaac caaggtattg ctgtaaatcc aaaatttacg
    15721 tatgtattgg tggaagccga tggccgtaag tttgtcgtag cgaaagattt gctagaaaca
    15781 gtccaatcag aaatcggttg ggaaaatgta acagttctaa aagaatttgc tggacaagaa
    15841 atggaatata tgactgcaag tcatccattc tatgaacgtg aatcacttgt gattttaggc
    15901 gatcatgtta ctttagacgc tggtactgga ttagtccata ctgctccagg acatggggaa
    15961 gatgactata tcgctggtag caaatataaa ctatctgtag tgtcacctgt agactcaaaa
    16021 ggattgttta cagaagaagc acctggattt gaaggtatct tttatgacaa agcaaatcct
    16081 atgatcactg atctattaaa agaaaaagga gctttgctga agctagattt ctttactcat
    16141 agttatcctc acgattggcg gacgaaaaag cctgtgatct atcgggcaac tccacaatgg
    16201 tttgcatcta tcgataaatt ccgtcagaat attttggatg aagtagaaaa agtagagtgg
    16261 gttatcccat ggggtaaaac acgtttgtat aatatgatcc gtgaccgcgg cgattgggta
    16321 atttcccgtc agcgtgcatg gggagtacca cttccgattt tctatgctga aaatggagaa
    16381 gcaatcatca caccagaaac aatcgaacat gtggcaaaat tatttgctga acatggatca
    16441 attatctggt ttgaaaaaga agcgaaagaa cttcttcctg aaggatttac ccacccaggt
    16501 tctcctaatg gcgaatttac aaaagaaaaa gatatcatgg atgtctggtt tgattctggt
    16561 tcttctcatg aagctgtttt acgtcagcga ccagaattat cattcccagc agatatgtat
    16621 ttagaaggat cagaccaata tcgtggctgg ttcaactcaa gtatcacgac tagtgtggct
    16681 atcaatggtg tagcaccata caaatcagtt atttctcaag gatttgcttt agatggtgaa
    16741 ggccgtaaga tgagtaaatc tttaggaaac atcattgcac cagaaaaagt gatcaaacaa
    16801 tttggtgcag atatccttcg cttatgggta tctagtgtag attatgaagc agatgttcgt
    16861 gtttctatgg atatcttaag tcaagtggca gaagtatatc gtaaaatcag aaatacgatg
    16921 cgtttcttat tagcgaatac agaagatttc gatcctaaaa aagatactgt ttcgtacaat
    16981 gatttacgtt ctgtagacaa atatatgacc gttcgattga accaagtaat caaagaaatc
    17041 cgagaagaag ggtacgacaa atataacttc atgcatattt accgtacagt tatgaacttc
    17101 ttgacggttg atctatcgtc attctacctg gattttgcta aagatgtagt ttatattgaa
    17161 gcagaagatg attatcaacg tcgttgcatg caaactgttt tctatcaaac agctgtagct
    17221 ttgacaaaat tattgactcc tatcattccg catacagcag aagaaatctg gtcattcctg
    17281 aaagaagaag aggagtacgt tcagttaagc gagtttcctg gttatgaaga gtttgcaaac
    17341 caagaagaat tgatggatac ctggactgct ttcatggact tccgtgataa tgtcttaaaa
    17401 gcattggaag aagcaagaaa cagcaaattg attggtaaat ctttggaagc aaaactaact
    17461 gtttatccaa aacaacaagt tcgtgaaatg ttaaaagctt tggatgcaga tattgcacaa
    17521 ttattgatcg tttcaccaga ctactttgaa gttatggcag cagatgaaca agtgccagat
    17581 aacgctatgg tatttgatga tgttgctatt acagtggaaa aagcagacgg ggaaacttgc
    17641 gatcgctgtc gccaagttcg taaagatgta ggagtagatg aaaaacttcc tcatctatgc
    17701 ggtcgttgtg caaaaatcgt tgaagacttc tatcctgaag ctgtagctga aggattcgaa
    17761 gaaaagtaaa agggaaaagg gggtgtgaca tgttctttgt cacaccccct tttgttatca
    17821 atcgtttaat tttccacgtt cacgatacca gttatctggt tgccagatcc attcgaatcc
    17881 gtctttttca agtagttcga aagctgcttt aggtcccatt gatccagctt tgtagttggg
    17941 aaaatccggt tgtgtttggt cccaagcttt tcgaatgaca tccacgatat gccaagattg
    18001 agcgacttct tcccaatgag agaagttcgt tccatcacca ttcaacgcat ctaatagtaa
    18061 tttttcgtaa gcttctgggc tattttcaac catttctgca ctatttctaa attccaattt
    18121 gattggttcc gtttcaaatc cttgccctac ttctttccca ttcaaggata aagaaaatcc
    18181 ttcagttggc tgaatataga tcgttagaac attaggtgga agtgtcgtat cgtcacatgg
    18241 ttcatcgaca gatgttttga atacattaac aggaacttgt ttgaatacga tgttgatccg
    18301 agtccctttt tcagtcaaac gtttgcctgt acgtacatag aaaggaacac ctgaccaacg
    18361 gaagttatcg atagtgaatt tgcctgcgac gaatgtttca gtctctgatt gagcatccac
    18421 tttgtcttct tcgcgatagc ccttgaattg tacaccatcc aattcacctt gagcatattg
    18481 cccgcgaacg aagttttcta acacttcttc ttcgttataa atgcggatag ctttcaatgc
    18541 tttgatcttt tctgtacgga tttctttttc ggaaaatgct actggcggtt ccattgcaag
    18601 taatgctaca acctgtaaaa tatgattttg caccatgtct ttaagtgcgc cactatgatc
    18661 gtagtagccc ccgcgatctt ctacaccgag gttttcgcca aaagtaattt ggatattgtc
    18721 gatatagcga ttgttccatt gagcctcaaa aatattattg gcaaaacgga tagctgagat
    18781 gttttggatc atttcttttc ctaaataatg atcaatcctg aaaatatctt gttctgggaa
    18841 tacttctctg atttgctggt tcaattcaaa agcagactgg taatcagaac cgaacggttt
    18901 ttctatgatc aaacgattga agccattttt tgtcataata ttttgtgatt tcaaatgaga
    18961 aacgatcgtt ccaaaaaatt gaggtgccat agccaaataa tagacacggt taccgtttaa
    19021 ttgatagtgt ccatctaatc ggtcagacaa ttcttttaat gtctgataat gcgttgaatc
    19081 atttacatca tgggattgat agtaaaaatg gcttgcaaag gtagttgctt cttcatctga
    19141 aggattcaat gcacggattg tttctttgac aacttcacgg tagtgctcat cgctccaggg
    19201 acggcgagcg gttccgatta cagcaaaatg ctcgcctaaa tttccttttc tgtaaaggcg
    19261 gaacaaagac gggtacaatt tacgctgggc taggtcacct gtaccgccga atatcgtaaa
    19321 caatacattt ttttcattag acatgtggat acactccttt atattttatc gacaaaaatc
    19381 gtgctggccg ccttaaagct gacggggatt ttctttctat cattttggat agtgatcggt
    19441 ccttcataag ctgcgatgtc aacgatctca tattcttcgt tgatattcag gtcaatagtg
    19501 actaagtaat ccaacaattc tttttcatca agcactcgtg cgatacgcac ttttgtaccg
    19561 acaggatagg actccaatgt ttgtcggcgt tcttcatgga caacttcctc ttttttagga
    19621 atcgatcctc catgaggaca atgttcagga tgattcaaat agttttctaa atgatctgca
    19681 agatgttccg aagtcacatg ttccaatact tcagcatcat catggacttc attccagcta
    19741 tagtctaaat gttcaactaa aaaaacttcc cataagcgat gtttgcgaat cagactgcta
    19801 gctttttgca gtccgcttgc agtcaattgt acaccttgat aaggggaatg ttctacaagc
    19861 tcttctttca caagtttaga aatcatttca ctgacagatg cagctgagac atctagacta
    19921 gatacgattt gtttattgtt gactttcgtt gtatctccac ctagttctaa aatgattttc
    19981 aaataatctt ctcgatttgg tgtcatattc gatcatcctt tcatggtaaa aaaccatcat
    20041 tagtaagtgt aacaaaaaaa acgttaattg actatttttc taaataaaaa acaaaggatt
    20101 tttagacgtt tttacaacaa aaaacgaaag atgatagaga agactcattc tttcgtttat
    20161 aaaggatata tatatacatt gggagggatc tatttttcag ataacaactt atatgaagtg
    20221 tctgcagatt cgtcataaag agtttgttcg ggataagaaa agataagata aaaaccatac
    20281 tgatcacgta cgactaaacg atattcatct atttctacca cagatgtgcc aagagactga
    20341 ccgttgatca aaaacgtgta atcagatgta atctgtaatt gttgtttttg ctggctatca
    20401 ctaaagaacc atgtaccttg atactttgag agtccttctg acttttccgc ttgttttttc
    20461 tttttcaagt aaataaaact tgagagaacg gtaatgagtg aaccaagaaa atagatcatt
    20521 ttttttatca ccaaatcact cctttatgag aaaataatcg catagacata ttctttcttc
    20581 tttatttcat tgtaccgaat aaaagctaaa atttctctaa gactttcgac tggttttttt
    20641 actgtttttc tacaaattat gataggatac atagtaggaa gaaaggtgac ataatttgaa
    20701 gtatcagcaa gtggaaatag ctttttttat tgaccgcccg aatcggttta tagcacattg
    20761 tatgaaaaaa gacggagaaa taattattgc gcatgtcaaa aatacaggta gaggaaaaga
    20821 ggtcctgata ccaggcgctg aagtggctat tgtatttgct cctggaccta aacggaaaac
    20881 cgcctatgat ctgattgctg taaaaaagaa gggtgactgg tttaatatcg atagtcaatt
    20941 gcctaatcgg ctggcaattg atggtatttt agacggcacg atccttttac caggattaac
    21001 tggtccattt acggtataca agcgtgaagt aacttatctt cactccaaat ttgatattta
    21061 tttagagacg gtggtcggac aaaaagcatt tgttgaagtc aaaggtatga cacttgaaaa
    21121 taaagcgatt ggtgcttttc ctgatgcgcc cacactcaga ggattgaaac atataggaga
    21181 attaacgtat gccgcacaag atggttatgc cgcttatgtc ttgtttattg ctcagttcga
    21241 acatcttcat ctggcaacga tccatgaaga aatgcaacca gcactagctg atatggttcg
    21301 acatgcgcag caaagcggag ttcaaatcct cgcctataat tgtcaagtat cggcagatga
    21361 ggtaactgtc aagcaggcca ttccatttga tgtagatatc ccttttgaag atcctataca
    21421 atagaaagaa ggtaaaataa tgatcaactt aggtattatc ggaacaaatt ggattactca
    21481 tcaatttgtc caagcagctt taagcacaaa aaaatatgaa ttgacagcgg tatattctag
    21541 acatttggaa aaagcacagg aatttggttc tcattatgaa ggggatatcg aattcgcaac
    21601 agatttgaag acttttttca atattactca tatggacacc atttatattg cttctccgaa
    21661 tagtcttcac tttagtcaag ctaagcaagc aattttggca ggaaaaaatg tgatagttga
    21721 aaagccagct ttttcgacgc caaaagaaat ggatgaaatc atccatttgg caaatgaaca
    21781 caatgtgtat ttttttgaag cagcaagaaa tatccatgaa tcaggtttta aaaagattgc
    21841 cgattactta ccattaaaag acgagataat cggtgcaaat ttcagctata tgaaatattc
    21901 ttcgcgctat gaccaagtat tagatggaga agaaccgaat atcttctcga ctcatttctc
    21961 aggtggtgca ttgatggatt taggtgttta ccttgtatat gcagctgttg cttggtttgg
    22021 aatgccaaaa gaagtccatt atttcccaag aaagatttct actggtgtag atggtcttgg
    22081 atgggggatc ttacgttatg agaattttga tgtatcgatc cagccaggta aaatcggtga
    22141 ttcttattta ccgtcagaga tttattttga ctcaggaacg ctagtgatga atggagtcaa
    22201 tgcaattgaa caagcacaat ttcatgatcg cgcgcataaa gaaatcattg atttggcggt
    22261 aacagctgca gaaaatccaa tggcagatga agcagaggat tttgccaatg tcatggctca
    22321 tccgaaagat ccagcttggg gattgttgta tgaagagtgg atcgagctag ctagaaatgt
    22381 gaaccaagtg atttatgatt taagaaaaaa tggcggcatc aaattcgatg cagataatga
    22441 ggaagatttt taatgaacga atttgtacaa gtatttcccg aaaattggca gaaaaaatat
    22501 gaggataaag ggtttatctc accttctctt attcaacgag cagtttttca gccactaagt
    22561 aataaacaaa gtatagtagc ggtttctcct acaggttcag gtaaaacact ggcttatctt
    22621 tggcctctac tactaaacgt ggaaccaggt gaagcaagtg cgttggtcat ttttgcttct
    22681 tctcaagaac tagctattca agtagcagat gttgcacgtg aatggggaaa agacaaagag
    22741 ttgaaagtcc aatctttggt gggcggagcc aacgtcaaac gtcagattga gggtttgaag
    22801 gaaaaaccag aaatcttgat tggtacacca ggaagaatat tggaattgat gaaagcaaaa
    22861 aaaataaaag cacatcaagt gaaaacaatg gtatttgatg aagcagacca gctatttgac
    22921 gctgggaaca gccaaatcat tgatcaaatt cttcatcaag ctccaacaga gtatcagcta
    22981 gctttttttt cagcgacagc agatcgttcc cttgaaagca ttgaaaaaat cactgggaaa
    23041 acgtttgaga caattgacgt gacagcagag gacgattctc gaaaaggact gagacattac
    23101 tttttacgtg taccgatacg aaagaaagat gactacttga gacgtctcac gcatatagaa
    23161 gattttcatg gtctgatttt ctttaatcaa ctgactgaat taggcgtaat ggaagaaaaa
    23221 ttgctgtata gaggtgtacc ggtaggaagt ctggcctctg atcagaacaa gctactccga
    23281 aaagcagcgc tagaacaatt caaaaagaaa atggtcagag cacttttgac cacagatgtg
    23341 gcggctagag gtttagatat tacaggtctt ccttatgtag tgaatgcaga agttccgtta
    23401 agtgaagaag cttatttgca tcgttcggga agaactggtc gaatgggtaa tgaaggaata
    23461 gtaattacac tcgtacaaga taataatcta agagatttaa aacggctttt acgtccatca
    23521 aatataacac ttgaagagat ttttttatac ggaggtaagc tgcaaactga gcagccaacc
    23581 aaagaagatg cagctcctgc agtaaataaa aagtatgcga aaactttttc tggaaaggag
    23641 cagaacccag aatataacaa agaaaaaata agaaaaaaag ttaaaaataa atcaaaaaaa
    23701 gagagaaata aaggagcaag gcgcaaataa gcgccttttt atgctatgat tatgaaaacg
    23761 ggaacaaaat tggttgccag aggaggggtt atgtgaaaga acgtcaagaa gagatcttgc
    23821 agctattgac agaaaaccaa caaggattga cagcaagtga tgtagctgag cgcttaacta
    23881 tcgatcgaag caatgcaagc cgatatttaa gtgaactata caaggcccat catattgtaa
    23941 aaaccgctgg ccgaccagtc gtttactctt tgccaacgga aaagtcaaaa tctgatgaag
    24001 tgcatgtcga ctcttctacg caagtgactt ttgaaacact cgttggagaa aatgattctt
    24061 taaaagtcag catacagcag gcaaaagctg cgattcttta tccaccgcgg gggttgcata
    24121 cgattatttt tggagaaact ggtacaggga agtcgatgtt tgctgaatgt atgtatcatt
    24181 ttgcaatcga ttcagagatg ctttctgcag atgcgccatt tgtttctttc aattgtgcgg
    24241 attatgctca aaatccgcaa ctgctctttg gacacatctt tgggatcaaa aaaggggctt
    24301 acacaggagc agctcaagat agtcccggtc taatagccaa agcagacggc ggtatattgt
    24361 ttttagatga gatccaccgt ttaccgccgg aagggcagga aatgctgttt actttcattg
    24421 ataaaggtat ttaccgtccg cttggagaaa gtgcacaagt acatgaagca tctgttcaga
    24481 tcattggggc gacgactgaa tcttcagaaa gtttcttatc tacgttcaat cgacggattc
    24541 ctatggcaat caccttgccg aatcttgctt cacgttcact ggatgagcgt tatgaaatca
    24601 tctctctttt tatcaaacaa gaagcgaatc gtttgaatca acgtatcgat atcgaaaaag
    24661 aagcgattct tgccttcatg ctttatgatg cggaaggaaa tatcggtcaa gtgaaacgag
    24721 acttgaaact agtctgtgca aaatcttttt tacattaccg tactcatcat gagaaaaagc
    24781 taatcattcg aaaagaagaa ctttcccttc aagtacaaaa aggattactg aaaatcaagg
    24841 aaataccgga acgtctcgac cgattcatgg acagcaaaag tcagtatttg acatttgaac
    24901 caggatttgc tgatgttgtc tggtcacaag atccagaacg aaacatgcag gtctataacg
    24961 atattgagga aaaagtcact tctctttccg aaaccggtgt ggagaacatt gatctagaga
    25021 ccctcatttc aaaagatgtg gatgcttatt tccagacata tgtcaaagaa ttgactaaaa
    25081 gttcgattcc caaagatctt ttgccagatg aaatctggcg gttaaccaat caattgtatg
    25141 atgtggctga aaaacggtta gaccgtattt ataatgaaaa agctcgtttt gcttttgctt
    25201 tgcatctgca aagtacattg gatcgtgtga gagaaggtca tatgatcgtc cacccagatt
    25261 tgaataacgt acggaaaaat tttaagaggg aattccaggt agcaatcgat ctttcaacga
    25321 tcattgaaga agaagaacaa gttgagattc cttttgatga aatcggcttt attagtatgt
    25381 ttctttctat caatctagga gaaagtgagc ctgttaaaga aaacaaagta gatgttctca
    25441 tattgatgca cggcagacag actgcttcaa gcatgttgga gatggcacaa gaactattag
    25501 gaacaaaagt agggaaagct tttaatatgc cactggttat ggaagttcaa acaatgtatg
    25561 aagaagtgtt acaatatgtc aaaaactatc aagagcattt gacgaatggc ctactcttgt
    25621 taacagatat gggatcatta aacaccttca gtgatttgat ttatgaagag actggcattc
    25681 ggacgaaagc tatcacgatg acaagtacca tgattgtgat agaagcgata cggatggcaa
    25741 gtgttggaag aagcttagaa gatatttatc aaaatatcca gctttccttt gaatcgatcg
    25801 tacgtgatca gttcaaagcg gaaaagtctg aaactttaca gcgtaaaaaa gccgtaatcg
    25861 tgacatgctt tactggtgaa ggagtagctg ccaaattata tcagcgtatt atgccagtga
    25921 ttaatcaaaa taaggtagaa ctgatccaga tgcagttttt agaacgtgaa tcattcaaaa
    25981 aacacatcga tgccttattg gaagaatatg agatcaaagc aattgcggga acagttgatg
    26041 ttgattatca aaatatccca tttttttcag cctatgacgt ttttgatgat gaaaaactaa
    26101 atattttaaa gcgtatagct gatgatgaga tcccgacagc taaaattgtc gattcacttg
    26161 ctggatcatt aagcgatgtc ttttctattg atgaactgat ccgttcattg caaaagacag
    26221 ttcagcagat ccagaccgat ttgtcgatta tcgtggaacc tggagtagat actggaatcg
    26281 ttatccattt agccttcatg ttagacggat tgatcaaaca agaaagccat cggcaatttg
    26341 agaaactgtc tattttctca aaaatttatc gtttggaaat ggatatcgtt cgggcaaact
    26401 tgatggcact agaacgaaaa tatcaggtaa aaattccaga agatgatatc gcatttctta
    26461 cacaaatgtt tcttgaaaat aaaataaaaa aatgaacaca ctaatcatac tgtttacaca
    26521 ctaaacatct gtgtgcgcac tataaaaaag agctaaaaag catgaaaacg ctatttcagc
    26581 tctttttttt attggcatgc ttcttgcgtt atataaaagt gtaaaaaaat aaaaggagtg
    26641 ttaaaaatgg caaaaaaaac aatcatgtta gtttgttcag cagggatgag tacgagttta
    26701 ttggtcacaa aaatgcaaaa agcagcagaa gaaaaaggga tggaagccga tatttttgcg
    26761 gtctctgcct cagatgcaga taataattta gaatctaaga atgtcgatgt attattatta
    26821 ggtcctcaag tacgtttcat gaaagctcaa tttgaacaaa aacttgcgcc taaaggtatt
    26881 ccattagatg tcatcaatat gcaagactat ggcatgatga acggagaaaa agttcttgca
    26941 caagcagaaa atctaatgaa ataaatcaag tatgcagttg ggggtataac aacgtggaag
    27001 atcaacaaaa tctagaagcc atcatggggt taatcatgtt tggcggtaat gcaaagagcg
    27061 atgcaatgga agcgattgcg gctgctaaaa tcggtgattt tgagttagcg gataggaaaa
    27121 tcgctgatgc agaagaatca cttatacaag cgcatcatgc acaaacagac atgttgacac
    27181 aagaagcaca aggaaatcat atgcaagtga cattattgac tgttcacagt caagatcact
    27241 taatgacatc tattgcgttt actgatttag caaaagaaat catcgaactt tatcgtcgcg
    27301 tatacagcga ataaatacaa cttctttttt gaacggaaga agaaaaattt ttgaacggat
    27361 tttttatttt gttataagga gaactctctt tatataaaaa acttatagga ggaagtaaaa
    27421 aatgaacgga ctatcaagtt ggatggagaa atacatcctg ccggttgccg caaaaatcgg
    27481 agcgcaaaaa catttagttg ctttgcgtga tgcttttatc ggcacactgc ccgcaacaat
    27541 ggcgggatca gtcgctgtaa tgatcaatgc aattattcgt gatttaccgc aacagtttaa
    27601 cagtacctat gctgacagtt tagcagcaga caaaggaatc tttgccgtat tgaatgtcat
    27661 catcggaatc aatggttttg tctggaacgg tacactagca atcgccggat tgatctttgc
    27721 cttttcttgg gggtataatc ttgcccgagc ttatggagta aatgatctag ctggggggat
    27781 cgttggttta gctaccttga ttcaaggtat ttcctttggt ccagcattat cagcagcatt
    27841 agatgtgaat gttccttcaa atgtcgcaga tgcaatcaat agttctgaga ttggtgcaac
    27901 gattgcagat ggcacactgt cagttggatt atggggttgg ctaaaactag atcatttgaa
    27961 tggaaacgct tattttacag ttatgatcat gggagctatt tcagtcatta ttttttgtaa
    28021 attgatgctt gccaacatta cgatcaaaat gcctgattca gtaccaccag cagtatctaa
    28081 agcatttgca gccattttac cagcgacgat cgctttatac gtcattgcta ttatcaatta
    28141 tatcgtaggt cgtgtagctg atgggcaact gatcattgat ttggtgcaaa aatatattgc
    28201 agagccattc ctaggattat ctcaagggtt gggagcagta ttgatcgtca cagtatttgt
    28261 tcaagtattc tggttctttg gtattcatgg accaaatgta ttggcaccag tgctagaggg
    28321 aatctgggga caagcacaac taatcaatat tgatattttc caaaaaggtt ataacggaaa
    28381 aactggtaca ccagccgtgt tagatgcaat cgaagatgga aaagcttata tgtgggtccg
    28441 cggatcgttc gatgcttttg catggtttgg cggatcaggc ggaacgatcg tcttactgat
    28501 agctatcttg attttctcta aacgtgcaga ttatctaaca gtagctaaat tatccattgg
    28561 gcctggtatc ttcaatatca acgaaccagt gatgtttggt ctgccaatcg tattgaatgc
    28621 aatcatgttt atcccattct tgattgctcc agtagtagca acagcaattg gatggacagc
    28681 tacttatctt ggcctagtcg ctccggtatc gcagcaagtc acatgggtag taccgccgtt
    28741 cttattgtca ttcttagcaa caggagcgga ttggcgggca ccgatcgtaa cattagtatg
    28801 tatggtcgtg actttcttga tttgggcacc atttgtcatc tctgcaaaca aaatggaccc
    28861 tgccgcaggc gaacaacaat aagatgaacg tgaggagcgg gacgctttaa gtttcgttcc
    28921 tcataatgaa ttttcgagga ggaactgaaa tgattacaat tgccagtgca atgcaacaag
    28981 atgaaatcaa agaattgtta gaaggataca atgaagaagg aatgactttt acatttaaag
    29041 aaaaacaagg aatcaagctg ttctttgaaa caagtgcaga agatccggaa gttgcggcta
    29101 aaaaagcaaa ggaactaatc aaagcacagc cttggggaac agtcctttat tttcaatcaa
    29161 cagctgttta gaaaatggtt taaaaagata ggatggttga gatgataaaa aacggtttat
    29221 ttttatgtag tattggttct ttgatcgtgt tgtatagttt gaatccacat tcgcaaaggt
    29281 atgatttgta tagtatggtc acaggtcttt ttctaacagg attaggaatc tatttcttca
    29341 tgaaggggaa aaaaagagaa gaagcaaagg agaaaaataa ctaatggaaa tcacgaaaaa
    29401 attaaatatt ccagcatcgt ttttttacga taaagttatg gactctgtcc ttttcgatgt
    29461 acgtaaagcg accggcaaat cagtaactag aaaacagctg aaaaatttgg agtacgtcaa
    29521 acaattctcg caaaataacc gggctcgcat aaaaatcgaa gaagtcattg aaaaccagtc
    29581 ttataagttt cgtacatcga caacgaagaa tgaattcgtc gttcattatc aaatcaaacc
    29641 attggacgat tcgacctgtg aggttcatta cacagaaaaa atggagtctt atggcatgct
    29701 tcaaaagttg aatgatatgc ttttaggaac aatcttgatg tattttaaac ggagacgttt
    29761 caaaaaaatg ctagatatga tcgaagcaac gtattaaatc aatgaaacat ggtactattt
    29821 agttattgaa aaatgaagta agctagaagg aatttttcat aggaagatag atctttcctg
    29881 cgaatcctca ctagcttttt tgtaggaagg ggcagcaaaa tgacaaacaa agtaagagag
    29941 ctaggtgtat cttcgattga ggaaatgttt gatttagcca cttatgcatt taatggggca
    30001 gatacacctg aaaggcgaga acgttttcgt acacttgcgg agaattcttg gaattacgga
    30061 ttttttgatg aggaggatcg gctaacaagt caagtcatgg cgaccccttt tccagtgaat
    30121 ttttacggac aagagtacct aatggccggc attggctatg ttgcttctta tccagaagca
    30181 aggggacaag gcgggatcaa tcagatcatg gaaaagatcc ttgaagattg ccgtgatcgt
    30241 aaagtaagct tatcttatct ggcgccattc tcctatccct tctatcggcg ctatggctat
    30301 gaacaatcat ttgataaaat cagttatcaa ctaaacagtc gtgatgtgcc gtttatcaaa
    30361 aaaaaatctg gaaaaataaa gcgtatggat tttgaagatg caaaagctgc aatcagaaga
    30421 atttatgaac agatgccgga aaaccaacga gctggtctga agcgcgaaga ctggtggtat
    30481 acgtataaat tcaagcaaaa aggcaattac cagtttgctt tgcattttga tgataatgag
    30541 gaagcaacgg gatatatcgt ttatcagctt gcaacgtctt cctttattat tgcggagtgg
    30601 ggatttttgg atcatgatgc tttttgcagc cttgttcgct tcgtaagttc tcacaatggt
    30661 gcttttgaaa catttgaata ttcatgcggc tacggcggta atgatcaaaa ttatctatta
    30721 gaaaatccat ttgcttctgt aatgatcaca ccttatatga tggcgaggat cgtagatatt
    30781 tctttgtttt tggaaaatta tccatttaaa aagagacaag ctgcatttgc cctacaaata
    30841 gaagaagatc attatgcgaa atggaatgaa ggaatcgtgg aagtaacaat cgaaaatgga
    30901 aaaaatcatc tctcaaaagt agaacaaaca aaactcccaa tcgttcgagg aagtatccaa
    30961 catatcacgc agctattatt agggtatcgt aaagtggaag agttggtatt ccaagaaaga
    31021 atacaggtga caaaagaata tgaagaaacg ttaagctcaa tcttgccaca gcaacagcca
    31081 attcttaatg actatttttg ataaaaaacg ggtgatgaca atatttttgt caccaccctt
    31141 ttgtttttca tttggcattt gaaggaaaat gttaaagtaa atcctctttt ttcgtaaatt
    31201 tgaatttttt gttttctttt gttttagggt tttctttttt gtggtcttgg attgcttctt
    31261 tcgtaatctt ttcccgattt tttttagatg aatctaagcg ttctgcgtat actttttctt
    31321 ttggcttatc attcattgga aaggacctcc ctacattatt tcattctacc tctagtttat
    31381 catctgttaa ggactagtgg aaacgttttg cctaacttat aagctgattt ttttagcaga
    31441 taagcttgca agtaaaaaac aaacaagcta caattgattt attgaaattg ttcctgtagc
    31501 tcagtaggat agagcaatcg cctcctaagc gataggtcgc tggttcaatt ccagtcagga
    31561 acgtacaaat aacccctgta tcctttgcgg tacagggggt tattttgtat aagtagacat
    31621 tgtagtttct aaactgctat gtcctaaacg tttcgatacg ctaagtatat ttactccttg
    31681 ataaagtaaa atagatgcat gcgtatgtct tagtccatca ataaacagtc gaagagaaaa
    31741 tagggaaaaa ttgattacta aagaaaaact tactaggtcg acggtgtagt tgattttcat
    31801 acttcaatag attcgaatta aacgatttcg attggcttat accagtccta tccaacgaaa
    31861 gatatatcgt tcttaactct agcggtataa cttgaagctt tacgtaaagc caatgggaat
    31921 gaactggaga tgacttttta ttatttttag taatagctga tgggttaata cttacaactt
    31981 tccttttcaa agtaccatat aaaaaaacgg tggggttgtt tagcgaaata aaggatctaa
    32041 gccaatagct tagatccttt attctatgct ttatattatt ttttgcatgt gtgcgaaagt
    32101 atactcaagc ccaatcgcca tttcggaaaa tcggtactgt agagccatct tcacggattc
    32161 cgtcgatatc catcttgtca gatcctacca tgaaatctac gtgggtttga cttctattca
    32221 atccagcttc tgctaattct tcatcggaca tttctgtacc gcctttcaca ctgaaggcat
    32281 aagcagaacc tagtgctaag tgattcgatg cgttttcatc aaataatgta ttgaaaaaga
    32341 tgatgccaga ttgagaaata ggtgaagggt caggtacaag tgcgacttct cctaaacgac
    32401 gcgcaccttc atctgtatca agcagttttg ctaagacatc ttctccttgt tcggcagaaa
    32461 aatcaacaac ttttccatct ttaaaagtaa atttcatacc cgagatgatg gtgccagcat
    32521 aactcagtgg ctttgtactt gaaatgtaac catctactcg atgactgtca ggtgctgtga
    32581 acacttcttc cgtcggcata tttgccatga atttttctcc acgagcatta tagcttccag
    32641 cgccttccca aagatggttt ttaggcaatc cgataatgat atctgtaccg ggagcagtat
    32701 agtgaagggc agaaaattgt tctcgattca attcttctgc tttttttgct aatttttcat
    32761 catgcttctt ccatgctaag acagggtctt cttcatagac acgagtcgtt ttgaaaatct
    32821 gatcccataa tgcctcaact tgtttttctt ctggcagttc tgggaagact tttgctgccc
    32881 attgtttacc agccgcagcc acgacagtcc aactgacttt atttgcttga gttgccttgc
    32941 gtaaattcat tagtgctttg ccattcgctg attgataaga cgctactcga tggctatcta
    33001 caccagcaaa tgcatcggga tcggcagaaa caacgctgat cctgcttgct cctttttcaa
    33061 gccattcatc cgcttgatcg attttagatt gtggtacatt ctcaatacga tccgttgcag
    33121 catgtaaaag aaattctctt tggatctggt cgtcagtcca ttgaacgata acctcagcgg
    33181 ctcctagttt ataagcttct tgtgtgatca atcgagcaag gggtgcctgt tccacgctga
    33241 tctgtaaaac aactgtgtgt cctttttctg tggctacgcc agtttctgca attaagcgtg
    33301 catatttttt tagaagttca ttaaaatcag ataacataat aaaaattcct cctcaccatt
    33361 ttttctggta actattagca tgataccatt taattatgat tcaaacaatg aggctttaaa
    33421 taaaaaaagg ag
//
LOCUS       DAZULY010000005        33132 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000005 DAZULY010000000
VERSION     DAZULY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.005"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1007
                     /locus_tag="ACG81A_000144"
     CDS             <1..1007
                     /locus_tag="ACG81A_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5013553.1"
                     /translation="LPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQV
                     RVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTPD
                     SIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDDT
                     LLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTSTE
                     EGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQLP
                     DELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSAL
                     "
     gene            1032..2216
                     /locus_tag="ACG81A_000145"
     CDS             1032..2216
                     /locus_tag="ACG81A_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFS5013554.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(2653..4236)
                     /gene="abc-f"
                     /locus_tag="ACG81A_000146"
     CDS             complement(2653..4236)
                     /gene="abc-f"
                     /locus_tag="ACG81A_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFS5013555.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(4341..4469)
                     /locus_tag="ACG81A_000147"
     CDS             complement(4341..4469)
                     /locus_tag="ACG81A_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013556.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(4814..4957)
                     /locus_tag="ACG81A_000148"
     CDS             complement(4814..4957)
                     /locus_tag="ACG81A_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFS5013557.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(5179..5658)
                     /locus_tag="ACG81A_000149"
     CDS             complement(5179..5658)
                     /locus_tag="ACG81A_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5013558.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(5731..6015)
                     /locus_tag="ACG81A_000150"
     CDS             complement(5731..6015)
                     /locus_tag="ACG81A_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013559.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(6093..6287)
                     /gene="ssrS"
                     /locus_tag="ACG81A_000151"
     ncRNA           complement(6093..6287)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG81A_000151"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(6359..7645)
                     /locus_tag="ACG81A_000152"
     CDS             complement(6359..7645)
                     /locus_tag="ACG81A_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFS5013560.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            7783..8454
                     /locus_tag="ACG81A_000153"
     CDS             7783..8454
                     /locus_tag="ACG81A_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFS5013561.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            8420..8908
                     /locus_tag="ACG81A_000154"
     CDS             8420..8908
                     /locus_tag="ACG81A_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFS5013562.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            8921..9868
                     /gene="prmA"
                     /locus_tag="ACG81A_000155"
     CDS             8921..9868
                     /gene="prmA"
                     /locus_tag="ACG81A_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFS5013563.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            9869..10633
                     /locus_tag="ACG81A_000156"
     CDS             9869..10633
                     /locus_tag="ACG81A_000156"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFS5013564.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            10866..13079
                     /locus_tag="ACG81A_000157"
     CDS             10866..13079
                     /locus_tag="ACG81A_000157"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFS5013565.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            13090..13536
                     /gene="dtd"
                     /locus_tag="ACG81A_000158"
     CDS             13090..13536
                     /gene="dtd"
                     /locus_tag="ACG81A_000158"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFS5013566.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            13707..14966
                     /locus_tag="ACG81A_000159"
     CDS             13707..14966
                     /locus_tag="ACG81A_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFS5013567.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(15055..16002)
                     /locus_tag="ACG81A_000160"
     CDS             complement(15055..16002)
                     /locus_tag="ACG81A_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS5013568.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            16396..17688
                     /gene="hisS"
                     /locus_tag="ACG81A_000161"
     CDS             16396..17688
                     /gene="hisS"
                     /locus_tag="ACG81A_000161"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFS5013569.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            17703..19472
                     /gene="aspS"
                     /locus_tag="ACG81A_000162"
     CDS             17703..19472
                     /gene="aspS"
                     /locus_tag="ACG81A_000162"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFS5013570.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            19581..20456
                     /locus_tag="ACG81A_000163"
     CDS             19581..20456
                     /locus_tag="ACG81A_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5013571.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            20722..22605
                     /locus_tag="ACG81A_000164"
     CDS             20722..22605
                     /locus_tag="ACG81A_000164"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS5013572.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(22680..22964)
                     /locus_tag="ACG81A_000165"
     CDS             complement(22680..22964)
                     /locus_tag="ACG81A_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS5013573.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(22954..23688)
                     /locus_tag="ACG81A_000166"
     CDS             complement(22954..23688)
                     /locus_tag="ACG81A_000166"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFS5013574.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            23817..24440
                     /locus_tag="ACG81A_000167"
     CDS             23817..24440
                     /locus_tag="ACG81A_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFS5013575.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            24632..25753
                     /locus_tag="ACG81A_000168"
     CDS             24632..25753
                     /locus_tag="ACG81A_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS5013576.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            25760..28888
                     /locus_tag="ACG81A_000169"
     CDS             25760..28888
                     /locus_tag="ACG81A_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HFS5013577.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            28907..32101
                     /locus_tag="ACG81A_000170"
     CDS             28907..32101
                     /locus_tag="ACG81A_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFS5013578.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(32247..32954)
                     /locus_tag="ACG81A_000171"
     CDS             complement(32247..32954)
                     /locus_tag="ACG81A_000171"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5013579.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        10826 a         5974 c         7050 g         9282 t
ORIGIN      
        1 tgttaccaga aaaaatggaa caagggttta atcaaaacct ggaagctatt cagcttttac
       61 agaatgcatt gggaacttcc tttttagaag cctatgtcga aaatgcagaa aatttaatag
      121 atgattatca agtacgtgtg gtggatggag tgccgacaaa agagacaacg caacggatca
      181 cagctcttta tgaagagttg aagaaacttt ctttcgagcc agaagaatgg cgcagactgt
      241 ctcaattact tttattgaag ggcagccaga cagaacattt gcaggcgaac catcaactca
      301 ctcctgatag tatcggtttt ttatttgttt ttcttattga acaactatac acaaataaaa
      361 aagcaccggt taagattctg gatattgcgg caggaatggg caacttgtta ttaacagttc
      421 ttttaaacct atcgaatgcg gggtaccaaa cagaaggaat tggtgtggat attgatgata
      481 cattgttggc tgtcgcagct tctacaagtg atttgacaca agcaaatgtt caatactttc
      541 atcaagacgg gttgcaggaa ttattaatag atcctgtcga ttttgcaatc agcgatttgc
      601 caatcggtta ttatccgaat gacgaaaaag caaaagagtt tttaacaagt accgaagaag
      661 gccatagtta tgcacatcat ttattgctag agcaatcaat gaaatatgtg aagccggatg
      721 gttttggttt gtttttgatg ccgagtggat ttttagaaac ggatcaaagt gaagaaatca
      781 aaaaatggtt taaagaagaa ggttatcttc aaggaatgat tcaattgcca gatgaattgt
      841 tccgtaataa acaatcgcaa aaaagtattt tgatcttaca gaaaaaagga ccacaagcac
      901 aacaagtaaa agaagtcttg cttgtgaaac ttgcttcatt aaaagaacca gagaaggtga
      961 cggaattttt caacgagttt aaaaactgga aatcatctgc tctgtaatca ttcaacgaga
     1021 aggagagtca tatgtctaaa acaattgcaa tcaatgctgg gagttcaagt ttgaaatggc
     1081 aattatatca aatgccagaa gaaaccgtag ttgcgaaagg gatcgtcgaa cgtatcggtt
     1141 tacaagattc tatttttacc atcaagtacg gcgataatca aaaattcgaa caaatcatgg
     1201 atatcgaaaa ccacgaagta gcagtaaaaa tgctgttaga tcagttgatt gaactgcaaa
     1261 ttttaagctc ttatgatgaa atcaccggag tagggcaccg tgttgttcac ggaggagaaa
     1321 cttatgggga ttcagtagtg atcgatgaga aagtaatgga ccgcattgct gaattagcag
     1381 aatttgctcc tttgcataat cctgcaaact taatggggat caaagcgttt aaaaagattt
     1441 tacctgacat tttgagtgtg gctgtattcg atacatcttt ccactcaaca atgccaaagc
     1501 aaaattattt atatagctta ccggtgtctt attatgaaga tttcaaagta agaaaatatg
     1561 gcttccatgg cacaagtcat cgttatgtgt cggaacgtgc ggcaaaatta ttgaacaaac
     1621 cgatcgaaga actaaaaatc atcacttgcc acttaggaaa tggtgtatcg atcacagcag
     1681 tcgatggagg taaatccatg gatacttcta tgggattcac accattagct ggggtgacaa
     1741 tgggtacacg ttcaggtgat atcgatccag ctgttctccc atatttaatg gaaaaattag
     1801 agattgatat tgatgaaatg atcaatgttt tgaacaagaa atctggtctg ctaggcctaa
     1861 caggattgtc tagtgatatg cgtgatcttg aaaagaacta tgaaaaagaa catattcgtt
     1921 tagcttatga cattttcgtg gaccgcatcc gaaaatacat cggcggttat gtgacaacga
     1981 tgaatggtgt tgatgcaatt gtctttactg ctggtatcgg cgagaatgac ggacatgtac
     2041 gtagtgaaat catcaaaggt atgtcttggt ttggttgtga aatcgatcca gcgttgaatg
     2101 aattgcgtgg tgaagaagta gatatctcta caaaagaatc aaaagtaaga gtattagtga
     2161 tcccaacaga tgaggaacta atgattgccc gtgatgtgga gcgtctgcgc ggctaagcca
     2221 aagcattcgt taggataaaa gatgaaacgc atgcaagagg gaattcttgc atgtgttttt
     2281 tttgcggaga agaccgcaaa atggtaattt aacttcaatt tcccttcttt tttacaggat
     2341 cttcttaggg aattttgctt ggaaaagtgg tcaaaaggga aaagactggc attttattta
     2401 ttgattttca ggaaaatccc aatattttat tttcattcat ctttataagg gacattacat
     2461 atgtaaaaag aaaaaacatt tttagatcag cctcgtgggc tgtcgacttt attcttcgct
     2521 ttatgtttag ctagaaaacc gatccgaaac attcattgat cgaataataa aaacttgatt
     2581 ttccacctgt actttgcctg aatcgtgaaa gcacaggtat gaaaatcaag ttttttgttg
     2641 cgaaaaaacg tcctagtcaa tcagttggcc gagcggctgg cggctgatct gctcctgatc
     2701 aataacgagt gcttcttgga agtgttgctg caaaaagtag cggtcatggc tgactgctag
     2761 gagcgttcct tgatagtctt gcagcatttc ttcaatttct tctcgagcat agctgtcgag
     2821 gtgattggtt ggttcatcca aaatcaggac gttgattttt tctaaaaaga gtttcagcaa
     2881 cgccaaacgg attttttcac caccggataa atctttgatc cgtttaccca catcgctttg
     2941 ataaaagcca cttttggcta attgccgtcg agcgacttct tcgtttccag tcaatgtgat
     3001 ggcgtaagcc agcaaacggc tatcgggatc ggcaaactgc agctgctgcg gcaaataacc
     3061 gatttgcaag ctactaccta atttgatggt gccaccgtaa ggcttttcct caccgagtaa
     3121 taatttgagt aaggttgatt ttcctgcacc attgtcgccg atgatggcta aatgatctcc
     3181 acgatagact gcaaaactgc tgtctttgaa tagtacttga tcagcgtact gctggtgcaa
     3241 gttttgaatg atgaaaactt ctttgccaga ttttccacca ttagacaagc tttgcagccg
     3301 atttttcggg ggaggtggcg gtttgacgag tgtcagtttc gccaggcggc gttccagttc
     3361 tttggctttt ttgaagaagg actcattgtc tccttcatgt gcccactgac gaaaacgacg
     3421 aatcatgact ttcaaccgct ggacttcttt ttgctgcagt tcgtaatttt ttgtcagctc
     3481 agccaagcga gcttctttca agactcgaaa acggctgtag tttccgggat actcaatcaa
     3541 tgcaccttct tccagttccc atgtttttgt ggtgacttga tcgagaaatt gccgatcatg
     3601 agaaacgacc agaaccgatt tttttgtatt ctgtaaatag ttttccaacc agcgaatact
     3661 gttaagatct aaatgattgg tcggttcatc gagtaaaagt aaatcggcat cggctgctaa
     3721 aattttggct aaagcgactt taactcgttc gcctccgcta agttctgtta accgtgcttc
     3781 ctgacagtgt ggaatcccta agccttgcaa gatcgctgca atccggtctt ctaatacgta
     3841 accgcctagt tcttcaaagc gatgctgcag ctcaccgtaa cgggccagac acttctctaa
     3901 gtttgtatct ggttctgtca tcaatgcttc gtagcgactc atttgctgtt tgaggtcctg
     3961 tatctcagcg gcagaatgga gcaagtagtg gttgacggtc gcttcggaaa ctgtaagttc
     4021 ttgttcgacc gtcccgatct ttaagccttt tttgcgactg ataacaccgc tgtcgacacc
     4081 ttctctgccg gtcaagattt gtaaaaaggt tgatttgcca gttccgttga tatcgactaa
     4141 ggcaattttt tcaccagctt caatttgaca ggataaatcc tcaaataatg gattcccgcc
     4201 gattgttttt ttaatagaaa ttgcttgtat gatcatgatc tctctccttg agaccactca
     4261 aaaaggccat ggttatccct ggcctgtgtt atttaaactc agattatcat gagaaaaata
     4321 ccttgagatg gccattgatt ttacgtgaat tcaactgttt tggacaaact tgtcccgtca
     4381 acagcaactg taaaaccaat tgcatggcta aatgcgatat ggtatcgttg gtaattttca
     4441 ttacttgtta ttttagtcat gcgcatcata gtattttccc tccttcgtgt agttgtcttt
     4501 tccagtgtac ctgaaagtgt cagaaagttc aactagcgct gacatttact taatatttat
     4561 cgatgtgtaa ttctgtcaga gagctttttt tatctgactg agggtaatgt ctcgtggacc
     4621 acatcattgt attctgagct aaaccagcgt tcaggcgaaa tcatatggtg tttgataaaa
     4681 aactttgtct acatgacttc cttttgccat ttgcttaata ccgttgcatt aaacagctct
     4741 tctctttgga aatctcacca acatcgcgtt caggagtatc gttgtggttg caatggcaag
     4801 cggtagcctt tcatcaattc ttgaatgatt taccgaatgt agcacttgcc ttctcgcggt
     4861 acccgaaccc gaaataaaat tttagtttgg cgataaaatc ggtatccgcc atcgatactt
     4921 catatttttt cgtatcagat tcatcggaaa cttccacatt gacgaaccaa taagctttgg
     4981 catgcttagg tcgttttaaa ccacaccagg gcgactaatc gccctggtgt tcactttttt
     5041 tattttactt gaactcattg actgttttcc ctttatttta aacattataa agaccgatga
     5101 gtgcttgccg cgtcttttgt ttttatcacg gttttttgtg caaaaaaacc tgccgatagt
     5161 tggcaggtcg tctacgtgtt aagctttggt gtggtcttta gctggcaatt ggttttctaa
     5221 atcagttcgg acaacaagca catcgcaagc tgcgtttcta ataacatatt cagaaacaga
     5281 accaataaat aaacgttcta cagcattcaa tcctgtggct cctaacataa tcaaatctac
     5341 ttgattatct tctgggattt ctctggcaat aatttgtttt ggtgagccat attctacgac
     5401 agaggttaca ttattcaagc cagctttctt cgcattgctt tcataatctg ccaatgtttg
     5461 ctttgccatt tcagtagctt gttctgcaag cattccatca aaggatgaaa cggtttggaa
     5521 agaacgtgtg tcaatgacat gtgctaatag aagttctccg ttatttcgga tagctacatt
     5581 gaccgccttt ttaaaagcta attcagcttc atcagaacca tctacggcaa ccatgatttt
     5641 tttgtattgt tgtaacataa gcattcactc ctcaagtcaa tcgtattgta ctcttttatt
     5701 gtagaacact ttagggaaaa agagaattat ttactgcttt ggttcagcaa ttttttttgc
     5761 aaatcggata ctgaaggtgg cagaaacaat aattacgatc agtaaaaaga ccaaggcaat
     5821 aaagcgttga ttgaaaaaag ctgttaaaat acccattacg cctaaaacag catatgcagc
     5881 accgtaaaat tgtaggaatc cttgattttt atcatttttg ttgatcaata caaaaaagat
     5941 atcctgttta ttaaataaat aatagctaac gaataataat actaaagtgg cgataatcat
     6001 taaaatccta atcatgaaca aatcctccaa aataagcaac aaatagaaaa agcaatttgc
     6061 caagagcaaa ttgctttatt ctttaaaaga tggtaatctc cgttaatgcc gttgtttgtc
     6121 ctgtagtatt gatcccgcga attcaagcag gtgggtccca tggtcgtagc ttcgaactac
     6181 caaatgataa ggcatgttac cagcagtgac acaagacggg tcccaagata tcaataaaaa
     6241 tgttcggtca acacacaaag aggacaattc gaacatcaca gatttcccac ctctatagta
     6301 gcatatgaat ttaaaaagag caatgagaga gctcgcagaa acagaaccgt tttcttgtct
     6361 agttttttgg aggatgcttt ttccactctt ttatacggta atactgttga cccagagcct
     6421 gttcatattt ccctgtatct tttggctgat aatactgagc attcttcagt ttatctggta
     6481 aatattgctg attgacccaa gcctgatcaa aatgatgagg atactgataa cccacgccac
     6541 ggttgagtga tttcgcacct ttgtaatggc tatctctcaa atgatcagga acatctccag
     6601 ctttgccctc tcgaatatca gcaagtgctg catctaatgc catgtaggca gaattggatt
     6661 ttggcgacaa gcaaagatcg acgacgacgt ctgctagtgg gatacgtgcc tcaggcaatc
     6721 ccaatttttc agcagctaat acagcgttca ctgtacgagc cgcagcagca ggattaccta
     6781 aacctatatc ttcataaccg ataaccatca gacgccggca aatacttgcc agatcacctg
     6841 cctcaactaa acgggcgaga taatgaagtg cagcatccac atcacttcca cgaatcgatt
     6901 tttgaaaagc cgaaataacg tcataatgag catctccatt tttgtcatgg gtaagagctt
     6961 ttctttggac acattcttca atgatcgaca gtgtaagatg aatccttcct tctttatctg
     7021 aaggagtcga tagtgtagcc agttccagcc cattcaatgc actccgcaaa tcgccgttcg
     7081 ttgcacgcga aaggtgaaga agagcatctt cgtctaactg gattgcttgt tgtccaaggc
     7141 cgcgttcttt atcccttaaa gcatgttcga ctgccaactg gatgtcttgt tctgttaatg
     7201 gtttgacttc aaagatctgt gtacggctgc ggatcgctgg attgatggta atataaggat
     7261 tctcagtagt cgctccgatc aagatgattc ggccactttc taaatgagga agcagaaaat
     7321 cctgttttgt tttatctaat cggtgtactt catccaataa gaggatcact gtaccactca
     7381 tttttgcttc ttccgctaca acttgtagat ccttttttga atctgtagcg gcgtttagca
     7441 tgcgaaatgc ataattagta gagccggcga tcgcacttgc gatactcgtt tttccagtac
     7501 caggaggtcc atatagaatc atggaagaga gcatgcgagc atcgaccatg cggcgtatga
     7561 tttttccttc cccgactaga tgctgctgac caacgacttc atcgatcgtt cgaggtctca
     7621 tgcgataagc caatggctgt tgcataggat tttcctcctt cttgttttta cgtcccttaa
     7681 ttatatcata tttagcaaga tgcctagaaa gaacagcctt ctgttgtttt gttttcatag
     7741 cttatccaac taatattgtg tatgataaag taggtgagaa aaatgacgga taaacaaaag
     7801 atagatgaat ttttttggaa taaaacgacg ccagaagtgg cacgggattt attggggatg
     7861 tatttggagc atgacacacc agaaggaaga ttagctggat atattgtaga cgcggaagca
     7921 tacctaggtc cagaagacga ggccgcccat agctatggtt taagaagaac accgcgtgtg
     7981 cgggcaatgt atgaaaaacc aggtacgatc tacctttaca cgatgcatac tcatcggatt
     8041 ttgaacatca ttactcagcc agaagggatt cctcaaggag taatgatacg tgcaatcgaa
     8101 cccgccgcaa tgattgatca aatgagtaaa aatcgtggcg gaaaaactgg tccagatatc
     8161 agcaacggtc cgggaaaatt agtggaagca ttagcaattc ctcaagaact ttatggtcag
     8221 tctatagcag atagttcgtt acgcttagtt tttgagaaaa aaaagacacc aaaaaagatc
     8281 aatgctttgc ctagaatcgg tatcccaaac aaaggtgtat ggacagaaaa gccgttgcgt
     8341 tttgttgtgt cgggaaatcc ttatatctct ttacaaagaa aaaaccaaat cgagaaagat
     8401 tggggttgga ggaaagaaaa tgaaaaagaa ggatctatta acatatttag atgaaataat
     8461 tgaaaaacaa gtgacagact atgatgtagc tttagattgg gatacaaaaa atcatacgat
     8521 cgagatcgtt gttcgcttgt ttgcagaaaa taaagcacaa tttgagattg atgatgcaga
     8581 agggatcgtt tcgcaagaac caattatcga atttgaagac ggcgtacttc tttttaatcc
     8641 tcagaaatct gtttttgatg aacgcaatta tctagcagta atcccttatg aaggaaaaaa
     8701 agggttggca aaatcagtag ctgattcttt agtggaatat ctaaatgaag tattagctca
     8761 agggcaaagc gatttgcttg attttttgga tgaagacgat gacgaagctg tattcgagct
     8821 tcactgggat aatcaacaac tagcagaatt agtagaagta aaacagcaaa atgaagcaga
     8881 tacttattta ccgtacccaa gttattaagg agtgacagat atgaaatgga atgaagtaaa
     8941 agtagaaact gaaagtgaag cagtcgaagc agttgctaat attttgatgg aggcaggggc
     9001 gagtggtgtc gcgattgaag attctttaga tgttgaacat tttcagtcgg atcaatatgg
     9061 tgagatttta gataaaaatg attttacatc gctgaaagaa ggcgcactag tcatggctta
     9121 ttttccggaa acgactttct taccggaaat cttgcctttt atcaaagatc gtatcacaca
     9181 attacctgag tttggtctag ctattggaaa aaataaagta acagttagtg aagtagagga
     9241 aagcaactgg gcaacagcct ggaagaaata ctatcatccg gtgcgtatta cgcgaatgct
     9301 gacggttgcg ccaagctggg aaaactacca gacagaagat cctctagaaa aaattatcac
     9361 actagatcct ggtatggcat ttggcacagg cactcatccc acgacttcct tgacattgac
     9421 agcactagaa acagtcttgc gtggtggtga aacattgcta gatgtgggaa ctgggtcggg
     9481 cgtgctaagt attgcagcaa aatatcttgg tgcaaaagat gtctatgcct ttgatttaga
     9541 cgaagtagcc gttcgttcgg cgaaagaaaa tatggatatg aatgaaatag ccaaagatgt
     9601 ccatgtatca gctaacgatc ttttgacagg aatcgagatt gaaagtgatg taatcgttgc
     9661 gaacatttta gcagatatca tcttattgat gatcccagat gcttggcgtt tactgaagca
     9721 aacaggcacg ctgatcgttt caggaatcat tgaagagaag aagcaaatgg ttctggatgc
     9781 catgttagaa caagggttta tcgtggatca aattttccag caaaaagact ggtatgcaat
     9841 tattttgaaa aaaccagagg tggaatagat gcagcggtat tttttaaaag aatcatatga
     9901 atcaaaagac tattttgttt tagaagggga aaactatcac cacattgtac gggttatgag
     9961 gatggaaccc gaggatgaag tttttttggc attcaatgat caaatagcca ttgttgcaga
    10021 aatcacggag attactgaag aggctgttta tctgaaagaa attaaaaaag aagaaaatca
    10081 aaaggaactg ccaagttcga ttaccatagc gagtggctat cctaaagggg aaaagctgga
    10141 ttggatcgtt cagaaatcca ctgaattagg agcgcaagct tttcttggtt tcccggcatc
    10201 agcctctgtc gttaaatggg atgcaaaaaa acgtatgaaa cgacaacaac ggcttgaaaa
    10261 gattgctcaa gaggcagcgg aacaatccca tcggcagatc gtgccatcag tagagttatt
    10321 ggaaacgaaa caagatttat ttgagcggct agatgattat gacgtggtct tggttgctta
    10381 tgaggaatca gcaaagcaag gcgaaaaatc aaatgcagcc aaaattctta gtgaaacaaa
    10441 accagatagt cgaatcttag ctgtctttgg accagaaggt ggattatccc gcgatgaagt
    10501 cgattatttt gtagaacaca aagggatact ttgtggatta ggaccaagaa tccttcgtac
    10561 agaaacagca cctctttatt tattaagtgc tgctagctat caatgggaat taatgaatcc
    10621 ttctttcagc taacgttctt cattaataaa agagtatcga cagaaagaat tgaaaaaata
    10681 gtcaacaatt tgtataatag aaacaattat ttatacaatg gatacgaaaa atcgtttgaa
    10741 aaagctttta aaaagcgaac atttccgtaa aaaaacgtat aactgcacgg attttgggac
    10801 aattatcaaa cagatatttg tcccgattcc cgttttaccg aaaaaagaaa gggggatcga
    10861 ttcctgtgac aaaagaagaa attttaacgg gacccggtgt cataaagctc gttagtcaat
    10921 acatgggtcc cgaacatgta gcatttgttg agaaagcatg cgaatacgcg actgctgcac
    10981 atgacggaca gtttagaaaa tcaggcgaac cctacattat tcatcctatc caagtcgcag
    11041 gtatattagc agatttaaaa atggatcccc atacagtggc tacaggcttc ttacatgatg
    11101 ttgttgaaga tacagaaatc actttagaag atctgagaga agaatttggc gatgacgttg
    11161 ctatgttagt ggacggcgta accaaattag ggaaaataaa atataaatcc cacgaagagc
    11221 agctagcaga aaaccaccga aagatgctgc tagcaatggc tcaagattta cgagttatca
    11281 tggtcaaatt agctgataga ctacataaca tgcgtacgtt gaagcacctg cgagaagata
    11341 agcaaagaag gatcgctcag gaaactttag aaatctatgc gcctcttgca catcgtctag
    11401 ggatcagccg gatcaaatgg gaattagaag atacagcact tcgttatcta aatccaaaac
    11461 agtattaccg tatcgtccac ttgatgcaga cgaagagaga agaacgtgaa aaatacgtaa
    11521 gtggtactgt tgaagatatt cgaatagcga cagaagagct ggggattttt gcagaaatct
    11581 atggacggcc aaaacacatt tattcaatct atcgtaaaat gaaagatcag aaaaagcagt
    11641 tcaacgaaat ttatgacttg ctagctattc gagtgatcgt agattcaatc aaggattgtt
    11701 atgctgtatt aggagcaatc cacacaaaat ggaaaccaat gcccggcaga tttaaggatt
    11761 atattgccat gccaaaagct aatatgtacc aatctttgca tactaccgtg atcggtccgg
    11821 caggaaatcc ggtagaaatt caaatccgaa cacaggaaat gcatgaaatc gctgaattcg
    11881 gggttgctgc acactgggcc tataaagaag gaaaaaatga aaaagtagaa ccagatggta
    11941 tgacgaaaca attaagctgg ttccatgaga tactcgaact tcaagacgaa agctatgatg
    12001 cttctgaatt tatggaaggc gtaaaaggag atatctttag tgataaagtc tacgtcttca
    12061 caccaaaagg agacgttact gagttaccaa aaggatccgg accattggac tttgcataca
    12121 gtatccatac agatatcggt aacaaaacca ctggtgcaaa agtaaatggc aaaatggtgc
    12181 agcttgatta caaattgaaa aacggagata tcattgagat catgacttct ccaaattcat
    12241 ttggcccaag tcgcgactgg ttgaaattag ttgctactag caaagcaaga aataagatca
    12301 aacgtttctt caaagcccaa gatcgagaag aaaatgtgat caaaggccac gaatccgtgg
    12361 tcaaatgtat tacagatcta ggatttacgc ctaaagatat tttgacgaag aacaaactgc
    12421 aagaagcact cgatcgtttt aattatcaaa cagaagatga tctctatgca gctgtagggt
    12481 atggagaagt tagccccttg acgatggcca atcgtctgac tgaaaaagaa cgtaaagaac
    12541 aaaaaatcga gcagcaaaag caagaagcag aagaaatcat gaatcagccg aaaaaagaac
    12601 ctgacaaaat gaaagtacgt catgaaggtg gcgttgtcat tcaaggggta gaaaacttac
    12661 taattcgtat cagtcgctgc tgtaatccga ttcctggtga tgatatcgtt ggttatatca
    12721 ctaaaggcag agggatatcc attcatcgtc gagattgtcc gaatgttcag cctgacaaac
    12781 caaatgtagc agaacgtttg attgaagtcg aatgggaaga tacatcgaat acacgaaaag
    12841 agtatgatgc agatttggaa atttacggct ataatcgttc aggcttattg aatgatgtac
    12901 ttcaaacagt caatgcgcta acgaaaaatc tcaacagcgt tgaagcacgg acgaataaag
    12961 ataaaatggc gacgatccat ttgacggttg gtatccagaa tttatcccac ctaaagagta
    13021 tcgtggataa aatcaaagca gtacctgatg tctacagtgt acgccggacg aatggatagg
    13081 aggacagata tgagagcagt tgttcaacgg gtaagtaaag ctgctgtgtc agtcgatcag
    13141 caagaaattg gaaaaattgg ctacggccta cttgttttat taggcattca tgaaaccgat
    13201 acgcaaaaag atgtggacta tctggtaaaa aaaattgcac aaatgcgtat ttttgaagat
    13261 gaacaaggaa aaatgaatat tagtgtagaa gatgtgggag gacaaatttt aagtatctct
    13321 caattcacac tgtttgcgga tacgaaaaaa ggaaatcgac caagctttgt ggaagccgca
    13381 cgaccagaaa cagctattcc tttatacgaa tcattcaatg aaggaatcag aagtcgcggc
    13441 atcacagtcg ctaccggaca attcggagca gatatgtcgg tttctctcgt caatgatgga
    13501 cctgtaacga tcattattga ttctaaaaac aaataaagta atctggtttt cactagtaaa
    13561 aactatgaaa cacgtcgctt aagttagact gataaactct aacttaagtg gcttttttgt
    13621 ctgcaaaaag cgcttctttt gtcagcagtc ggaaaaaaag ttaagcttaa attatacaag
    13681 ttatttttta taggaggttg ctgaagatga aagaagtacc agtagtagat atcaaaatca
    13741 cagatttaaa aaaattgtat caaaaaggat acaacatgtt ggaggagtta cgccatgaag
    13801 cggacgctcc cgtcgtcaaa gcaaagattt tcaataaaga agctatcacg atctacggat
    13861 catcagcagc aaaagtattt tatgatccaa ggaatttcaa acgaaaaggt gctatgccaa
    13921 aattagtatt gaagacactt tttggtcaag gtggagtaca gactttggat ggagctgctc
    13981 atcaccatcg gaaaaatatt tttatggatc taatgacacc ggaaagaatg gaagattatc
    14041 atcgtatctt agataagaat ctgacacaag cacttgaagc acagcatggt caatttgaac
    14101 tatttgattt aagtaagatg gttttcttta catctatttg cgagtgggca ggtatcaatt
    14161 tatcagcgat ttctaaagat gaggtcgaaa aattagcaga atatcaaatc tctatgatca
    14221 gcggaacgtt tacttctccg attgatcaca taaaaggtgt cgagaatcgc aaaaaatcag
    14281 aaaaatgggc gcaaggcttg attgaagaag ctcgacaaaa tcctgtggct ggaaaagaaa
    14341 atgtggcatt atatgctttt gccaatgcca cagatttaga tggtcagctt ttacctctgg
    14401 aagtggcagc tgttgaatta ttgaacatca tccgtccaac ggttgctcta actgtgtggg
    14461 cggcattgat gggacatgca cttttcagta gaccagattt gtatcagcaa ttaaaaaatg
    14521 acttttcgac gttacaagat ccgtttattc aagaaatgag acgctattat cctttcttcc
    14581 caatgctacc tgcgatttcg ctgaaagaag tagaagtaga tggttatcgt ataccagaag
    14641 gcagttgggt tattttagat ttatatggaa cagaccatga tgaacgaaca gttgaagcgc
    14701 cagattcctt tatgatcaaa cggtatgtag ggaaagcaaa agatatttcc tataaagaag
    14761 aatatgagat gattgcacaa ggcgggggaa attttcgaca aatgcatcgc tgtgcaggag
    14821 aatggatcac tcttcatagt ctacgtgtgt tttccgatca gctagtaaat aagtttgagt
    14881 tctctgtacc tgaacaagat tggacgattc cattcaatca gtttccgact tatccgaata
    14941 gccgagcgct actttacaag aattagatta gcataaactt atcgaaaaaa aggaagatca
    15001 aacagtgctg atcttccttt tttcatatta ctataaattg ataaggtcaa ttgatcaaaa
    15061 tccacgaaat gcttcatcaa tggtttgata atcttcattg gataaatgca aatctaaagc
    15121 ttggacattg tcagatactt gctctggacg cttagctcca ggtataacta cagacactcg
    15181 tggattcttc atgtaccaag ctaaaacaag ttgcgcagga gtggcttggt atcttttagc
    15241 aatggggcga agtacatcga cagcggttaa aatttctctg aaacgttctc cttggaaatc
    15301 tggatttctt tttcgaggat ctccttcacc aaattgtttt tcctcaccaa gttcatattt
    15361 tcctgtcagc aaaccggaag caagaggaaa atagggaaca aaactgattt tgtttttttc
    15421 aagataaggg aataattctt tttcagcctg ccgatggatc aaactgtatt tatcttctac
    15481 tacatcgaca tatccatctg cattcgcttc tttcaattgt tctaacgtga agtttgacac
    15541 accgactgct cggatttttc ctgcttcttt taattcgtgc aatgtagcaa ctgattcatt
    15601 ttttggtgtg ctttcatccg gaaagtggat ataaaaaatg tcgatgtaat cagtttgcag
    15661 acgttttagt gcatcttcca ccgattgttt gagaaactca ggagaattat caaatgttcg
    15721 ccctttattt ggtacatgag cagcttttgt agcgatgacg acacgtgagc gatcatattc
    15781 tttttccttc agtacctcgc caatcagttc ttcggaacgg ccgtttccgt aagcataagc
    15841 ggtatcgatc aaattgatcc cgctattcaa agctgtacga actacttgtt ttcctgtctc
    15901 atcttccaaa ccgtcaaaaa gattatgtcc tccaacggca tttgctccta atccaagctg
    15961 ttctgcgtag acttgtgaat gtccgattct tatttctgcc atagctgatt cctccttttt
    16021 gtctagtcta aaacaaaata ccaaaaagct gaactaaaac gtttttgtga ggggaaatag
    16081 tcagatatct cttgactttg agactggttt ttagtaggtt ataagtaata tcgaatgtca
    16141 atgaaagagc gagtaagttt ttgaaacttt acaagagagg atcatcgagg ctgaaaatga
    16201 tctaaagttt cattagccta aagacactct ggagatggac gagtgaaaag ccgtcccgcg
    16261 tacgagcgtt attcgtaaga ggaaagagaa atctttcaaa attaggtggt accgtgtatg
    16321 aatttcattc acccttgttc atgatgaaca agggtgattt ttttatttac atagctaaga
    16381 aagggagaga aaactatgag tttccaaaga ccaaaaggaa caaatgatat tttgccaggc
    16441 gaatcagaaa aatggcagtt tgtcgaagaa acagcacgtt tattattcag agattatcag
    16501 tataatgaaa tgcgtacacc gatttttgaa cactatgaag tgatatcccg aagtgtaggg
    16561 gatacaacgg atatcgtttc aaaagagatg tatgattttt atgacaaagg ggaacgccat
    16621 gtgacgcttc gaccagaagg tacagcacca atcgttcgtt cttttgttga gcataaattg
    16681 ttcggcccag agtatgcgaa accgttcaaa actttttata tgggaccaat gtttcgctac
    16741 gaacgccctc aaaaaggacg gttacgtcaa ttccatcaaa tcggagtaga agcatttgga
    16801 tcagaaaacc ctgcaactga tgtggagagc atggtgatgg cacttgattt cttcaaacag
    16861 ttggggatca cgcaaatccg gctagtcatc aattcattag gtgataaaga aacgcgtcaa
    16921 gcctatcgtc aagcattgat cgactatctt ttaccttttg aatcagagct gagtgaagac
    16981 tctaaacgcc gccttcatga aaatccatta cgcgtattgg acagtaaaga caaacgagat
    17041 caaaaatttg tggcagaagc tccatctatc ttagattatc taagcgagcc tgctcaaaaa
    17101 cattttgata ccgttgtttc catgctggat gcattggctg ttccttatga aatcgacagt
    17161 aatatggttc gcggactaga ttattacaca catacgattt ttgaaatcat gagtgacgca
    17221 ccaaaaatgg gggcacaagc aacgatttgc gcaggcggac gttatgacaa tctagtggaa
    17281 gaactaggtg gtccttcaac acctggcttc ggatttgcaa tgggtatcga gcgattactc
    17341 ttgactatgg aggcagagga agtagttatc cctgcattta atgagttgga tgcttacgtg
    17401 gtcgcactcg gagacgaaac aaatatcgaa gcattgaaag tggtccaagc gattcgaaac
    17461 tttggttttt cagctgaccg cgacttcatg aaccgtaaag caaaagcgca atttaagaca
    17521 gcagataaag cgaatgccaa attagtattg gtgatcggtg aagatgagct tgctagtcaa
    17581 acgattaatg tcaaagcaat ggcaagccga aaagaaaaaa gttttccatt agaagagata
    17641 tatgaacatt ttgatgaagt ctatgatgaa atgactttat tcgagtagga ggaaaaaaat
    17701 aaatggcaca aagaacgatc tattgcggat tagtttccga acaacaatta gaacaaacag
    17761 ttacattaaa aggatgggta caaaaaagac gagaccttgg gggagtaatc tttatcgacc
    17821 tacgtgatcg tgaaggaatc gttcaaatcg tctttaatcc tgaaatcaac aaagaagcat
    17881 gggaaatcgc ggataaatgt cgcagtgaat acgtgatcga agtgactggg gaagttagaa
    17941 gacgcggaga aaatgcagta aatcctaaaa tggccactgg tgaattcgaa gtgattgcaa
    18001 gtgaaatcgt gattttgaat caagcaaaaa ccccgccatt tcaaattgaa gatgatcaag
    18061 caatcagtga cgaaatacgt ttgaaatatc gttatttgga tctgcgtcgt ccaaaaatga
    18121 ctaaaaacat caaattaaga aatgcgacaa caaaagcgat tcgtcattat ttagatgata
    18181 atgactttct cgatatcgaa acaccttacc tagggaaatc tacaccagaa ggcgcaagag
    18241 actacctagt tccttcacgt gtccatgctg gacatttcta tgcgttgccg caatctcctc
    18301 aattgtttaa acaattgctg atgaatgctg gattcgaccg ttattatcaa atcgttcgtt
    18361 gtttccgcga tgaagattta cgtggagacc gtcagcctga atttacgcaa gtagatattg
    18421 aaacaacgtt cttaacagca gaagaaatcc aaacttacac agagggattg attgcgaagg
    18481 taatgaaaga agttcgcggt atcgaagtca cattgccatt tcctcgtatg acctatgatg
    18541 aagcaatggc tcgctacggc agcgacaaac cggatacacg ttttgatatg gaattgatcg
    18601 atctaagcga tacagtcaaa gaagtagaat tcaaagtctt ccaaatggct cttgaaaacg
    18661 gcggtgtcgt gaaagcattg aatgctaaag gcgctgcaga tcgctactca cgaaaagaca
    18721 tggatcagtt aggtcaatat gtaggacaat tcggagctaa ggggcttgca tggctgaaag
    18781 tagaagaaga cggattgaaa ggtccaattg ctaaattcat gggagaagca acagaagcaa
    18841 tcattaaagc aacagatgca aaaccaggtg acttgctgat gtttggcgca gataaaagtg
    18901 aaatcgtcgc tgctgcatta ggtgctatcc ggacacgttt aggaaaagaa ttaggactaa
    18961 ttgatgaatc gaaattcaat ttcttatggg taactgattg gccacaattt gaattttcag
    19021 aagaagaagg tcgatatgtc tctgctcacc atccattcac gatgccaaaa gaagaagata
    19081 tcccgttatt ggctgaagac cctgcaaaag tctatgcaga agcttatgat atcgtcttga
    19141 acggttatga attaggcgga ggttctatcc gtattcatac tcgtgagctt caagaaaaag
    19201 tactagaaac tctaggcttt acaaaagaac aaatggaaga acaatttggt ttcttgttaa
    19261 cagctttaga ttacggattc cctccacatg gcggtattgc attaggattg gaccgttttg
    19321 caatgttatt agccggagaa gataatattc gtgaagtcat tgcctttcct aaaaacggaa
    19381 aagcagcaga tgtgatgagt gatgcaccaa gtatggtttc gcctttacaa ttgttcgaat
    19441 tgaatattga tgtaacgaat atagaagaat agccgataaa aaggtaaaaa ggatttgtcg
    19501 aatacgcttc ttttgacaac cttgatttaa ttgaaaaaca ctttataatg aagccataac
    19561 atataaagcg gggcttttcc atgaaaaaaa cattcgaaaa ttgggcttat catgattaca
    19621 cgaccaagct ttcggtatct atcgtttatg cgttgatcgc atcagtcgca ttgaatttct
    19681 tttatcagcc aggacatatt tattcaagtg gtattactgg tctagctcag attttgacga
    19741 ctttgtcaaa atcactgata ggattcgaag taccggtatc aacaactttg tatttactga
    19801 atataccttt gtttgtattg gcgtggttaa aaatcgggaa gaagtttaca attttcactt
    19861 ttatcacagt cactttgacc tccctttgta tccatatttt accggaaaca gtcctatcta
    19921 ccgatccaat catctgtgcg ttgtttgggg gggctgtgat gggactaggt attgggctag
    19981 cgttgagaaa cggcctatca tcgggcggtc tagatatcgt gagtatcacg attcgtaaaa
    20041 aaacaggaaa gtcgattggt tctatttcga tctacttcaa tgcattgatc gtttttgttg
    20101 ccggaattct atttggctgg caatatatgt tttacagtac gttgtctatt ttcgtcagtg
    20161 gaaaagtaac tgatgtcgtt tatactaaac aaaagaagat gcaggtaatg atcgtaacga
    20221 aacatccaga aaaagtagta aatgaaatcc aaaactgtat gcgtagaggg atcacgatca
    20281 tcaatgaagc agaaggtgct tataagcatg ataagcagac agtattgatg acgatcgtta
    20341 cacgatacga actgcctcta cttagagaag caatgaaagc ttctgatcca caagcttttg
    20401 taagtattgc agaaaacgtc cagatacttg gtcgtttcta tgaggatggc atttagatcg
    20461 agtttgacaa aaattcatga aatactgctg tagacagacg ttcgtttgtc tgcagttttt
    20521 ttctaccata gtagaatcag caaagaaaaa ctgctttttt atttgaaatt ttcttaaagt
    20581 tgaaaaaaag catgtttatt tttttaaatt tatgggatga tagaaaaaaa tagtagagaa
    20641 aggacacgat tctacttgct attttcactt aatacccgta tattacgaat gaaaaaagtt
    20701 tatgaaaagt tggaatattg aatggaaaaa caaaaaaggt taaaaaacag ccgctacatc
    20761 actgggtttg atgggctccg ttcattagct gtggtgggtg ttatcttcta tcaccttctg
    20821 ccaactagta tgaaaggcgg ctatttgggc gttccgattt ttttcgtcgt atctggttat
    20881 ttgatcacgg atctgctgcg gcaggaatgg gaacaaaatg gaaaaatcaa tatttggcaa
    20941 ttttatgtca gaagaatgaa gagactctat ccggcacttg tttttatgct tgtgacagct
    21001 tcggcttata tcactttgtt tcagcgaggg ctgttaaaca atcttcgagg agttgtggtc
    21061 agcagcttac tgtatgtcaa caactggtgg caaatcaata acggtttatc ttatttcgat
    21121 cgatttgcta atgagtcacc gtttacgcat atctggtctt tagcagtaga aggtcaaaac
    21181 tatttggttt ggccgatctt gtttgtattg ttgatggtat ttgttcgtaa aagaaaatgg
    21241 atcttttata ctgtgttagg tacatccttc ctctccgcca tcttgatgat gatcttgtat
    21301 acaccaggag gcgatccaac ccgtgtttat tacggtaccg acactcgtct gttttctata
    21361 tggttgggaa gtgcgcttgc gtttgtttgg ccaagtacgc gtttaaagaa aaacatcccg
    21421 aaacaagcga aaaatattct gaatatcgct ggaggaatct cgcttttggc actgattctt
    21481 tgcttcttct ttttagatga tcattacaat ttcatctatt acggaggaat gctcctagtg
    21541 agtctgttat gcgtagtgct agttgcagtg acagcacacc ccggagcaag tttgaatcga
    21601 tggctgacaa atccagtatt tacttggatt ggcaaacgaa gctatggcat ttatctgtat
    21661 cagtttccag ttatgatttt ttatgaagca aaggtgactg atattgccga tcatgttttg
    21721 ttgcatactt tagtggaaat cacattgatt ctaatcatca gtgaactttc ttatcgatat
    21781 gtggagcgtc cgctagcacg ttttgattat ggtcgtacat tccaagtgat gaaaggatgg
    21841 ttcagcaaac cgatcatcag taagagcaaa ccatgggttg ttccaggcac attacttgta
    21901 gcaattgcac tcgttggttt cgttacggca ccgaaaaacg ctgtaacagc cgatcaaaaa
    21961 caactgcagg caaaaatcgc tgaaagtaaa aaattagcag aagaaaccag aaacaattca
    22021 gaaaacacca ctagttcggt tgaccaagcg atccttgata agtacgagtt gtccaaggca
    22081 caagggaata aggcacaaaa attagaacta acggcatttg gtgattcagt tatgttggat
    22141 gctgcaacgg atctacaaga actttatcca aaagtagtgg tagatggaga tgtcggacga
    22201 caattatatg ccagtgtacc gtacattgaa aaattaaagg aacaagattt gttgaaagac
    22261 actgttctga ttggactagg aacgaatggc tcattctcgg aagctcagtt tgatagtgtg
    22321 atggatgcaa tcggtgatcg aaaagtatac tgggtcaatg tccgagtacc gacgaagcgc
    22381 tggcaaaatg acgtgaatgc gatgcttgaa cagatgacga aaaaatacga caatatgacg
    22441 ctgattgatt ggtacagcta cagtaatgac catagtgact ggttttatga tgatcaagtc
    22501 catccaaatc ctgaagggat gacacattac attcacttag tcagcgaagc cttgctagga
    22561 ggaagttcaa gtacgaacag cagtgagaca aacgtgacag aatgatagct acaaaaaact
    22621 tcaggcaaaa acatttgttt ttgcctgaag tttttttata tttaccgtgt ttttaacatt
    22681 catatagatt caggaagcaa aacggttgag tgtgtctgta aataacgttc tttttgtttt
    22741 cgggtgagtt ttttgaatgc agcttctgct tttgttgctt cgcttcttga agaaaatact
    22801 tctgcatgga tcatcttaac aggtcttctt ttttgaagac gagtatattt agcaccagta
    22861 ccattgttat gttcctctaa tcgtctagaa agatcggttg tatatccacc ataaaatgtc
    22921 tgatctttac agaataatac gtaaaaataa tgttcatttg ccatagagca tctcacgaat
    22981 ctctgcagtg tattctccat ccatattgta agtgatcaat ggtggagcga ctttgaatcc
    23041 gtctaatttg ccttctttga ttccttcaac taacaaaata tttgcctctt ttccaggttt
    23101 tgggtaaaca aattggatcc ttttaggtgc aattcgatag tcttccatga cctgaatgat
    23161 ttctaagaac cgatcaggac gatggaccat agtaaagcgt ccatttgttt ttagtagctt
    23221 acttgaaata tcgatcactt cttctaacgt cgtatgaatc tcatgacggg cgattgccag
    23281 gtgtttatta gggttcgttt tatttgttgg cagccctttg aaataaggtg gattacagac
    23341 gactaagtca caagaattat gtcgaacgac agataagctg tctttcagat cgatggtgtg
    23401 gacagtcatt tgttcattta aatggttaag cgcgatacta cgtcttgcca tgtctgctag
    23461 acgaggttga agttcgatag cgtcgatagc cgcctgggtt cgtttactta aaaaaagtcc
    23521 aactgcgcca tttcctgcac aaaggtcaac aatcttccct tttttaggta cacgggaaaa
    23581 gtgagcaagc agcacagcat ctaaagaaaa agaaaacact ttggaacttt ggataatctt
    23641 gatatcttct gcgtataatt ggtcgattcg ttcgtcttcg tgaagcatgg attccctcca
    23701 aaatgaaaaa aatagtcgtc tgtattatac tatttttatt aaaaagaacc aaggtggctt
    23761 gtcaaacctc tattttttat gcatactatg accataagaa gggaaaggaa gagactatgt
    23821 tttatcgatt tatgcgcggg gtagttcgag tactattagc cttagttaat ggaaatgccc
    23881 gctatgagaa taaagaagtt ttaccgcaag atgagaatta catactagtt gctccccatc
    23941 gtacttggtg ggacccatta tatctggcgg tagctgcagc acccaaggaa ttcagcttta
    24001 tggcaaaaga agaactgttc aaaaatccag ttctgcgatt tatattaact cgatccaatg
    24061 cttttccagt taaaagagaa aatccaggac caagcgtcat taaaacacct gtaaagattt
    24121 tgaaatcgac aaatttgagt ttgatcatgt ttcccagtgg gacaagacat gctacagaac
    24181 ttaaaggcgg aatggcattg atttccagaa tgtctaaagc gaaaatcgtg ccagctgtat
    24241 atcaaggtcc gttgacactt ggggatctgt tcaagcgtca gagagtgaca gtgcgtttcg
    24301 gcgaaccaat cgacttatca gatatcaaga aaacggataa agaaggtatg gcggagatcg
    24361 aacgacgaac acaagcagct tttgacaaat tggacaaaga agtgaaccct gatttcaaat
    24421 atgaagtcaa aaaaaaataa aatgataagt ttatgcgtgg gcggtctttt gaatctactg
    24481 tcctgttcag ttcagacttt ttcaatgaaa acgagggaga gacgtcaagt ctgccctctt
    24541 tttgcgttcc aatatgtaaa atagaagaca ggtgtcatgt aatggatcaa agtgttataa
    24601 taattagaag gacatttttt gaggtgattg aatgcgtttt ttacatacag ccgattggca
    24661 tatcggaaaa aaactacaag gttatgactt gattgaagac cagcgtcatg tactatcttc
    24721 tatactagat atcgcgaaag aagaaaaagt agaggcaatc gtgattgccg gagacttata
    24781 tgataggagt gtcccagctg ttgatgccgt agaattatac aatgaattga tggtcgattg
    24841 gaatttaaag gaaaagatcc cgatttttgc tatatcagga aatcacgata gcagtacgag
    24901 actttcagcg ggtacgccat ggttcagtta ttcgaactat tttttgcgaa cacagctagc
    24961 agaagcattt gaaccagtcg agtttaaaaa cgtacaattt ttctttcttc cttactttga
    25021 accaattgca gcgagattat attttgaaga tgacaatatc cgaacgatca agcaagcggt
    25081 agccaaggtg atagataaaa tgcaggagct atttgattca gaaaaaaaac aagtattgat
    25141 tagccatttt tttgtaatgg gaagtgtaaa atctgattcg gaaacgaaaa tcgaagtagg
    25201 cgggttggat tcgatccctg gtgaactact tacaccgttt gattatgtag cactaggaca
    25261 tttgcataat caagctgctt tgagacagga taatgctcgc tatagcggtt ccccattgaa
    25321 attctcttta tctgagatca accaaaaaaa aggcgtatgg atcgttgacc tgacaacagg
    25381 cctggagttg aattttaggg agatcgaacc cctgcatgat attgaagaaa taacagcgag
    25441 tttcaaagaa ttgctggatc ctgcctttta tcagtccgtc aatcgggaga attacatcta
    25501 tatctatctc actgatagaa cgatcatccc gaatatgatg aaccagcttc ggcaaatata
    25561 tcctcgtatt ttggggattg aacgggcaaa cggtcgagca gaacaaacaa agcgcaaacg
    25621 aaaagtacta gaaatgaaag atcctcaaaa attagcagaa gcatttttta gtgaaatgac
    25681 ggaagagcag ctaactgcaa accaagaaaa atggctcgga gaaacgttgc aagaaatcat
    25741 ggaaatggaa tgatcaagaa tgaaaccgaa aaaattacaa cttaaaaatt ttggtccttt
    25801 tatcaatgag accgtagatt tttcacgttt gacagaagca ccgctttttt tgatcagcgg
    25861 gaagacagga gctggcaaaa cgacgatttt tgatgggatc accttcgcac tttttggtga
    25921 aacttccgga agacttcgct ctggaaaaga gatgcgttct ttgtttgcta caccagacga
    25981 agaaacaagc gtgacttttt cttttgaaca tcagcagatg agctatgaaa tcgaacgaaa
    26041 accagaacaa gttttagcaa aacgaaaagg aaacggaaca aaaaaacagg cagccaaagt
    26101 aacgttgacc atttttgatg ggaaaggaaa agaaataaaa caaatcacaa aacgtacaga
    26161 agcagatcag ttgatcagag aactgatgaa tctcgatgcg aaacagtttt ctcaaatcgt
    26221 tttactaccc caaggagaat tccgtaattt tttggtttca tcaagcagtg ataaagagac
    26281 tgttttacgt catttattcg gtactcaatt tttccaacag ttcaatgagc gattaaaaga
    26341 aaaagcaaaa caacagcaac aaaaattgga tcatttagag caagagctgc atcttctgca
    26401 aaaaaggttt gttttagtca aagaagagga accagcagca gggtttgaag ccgttttagc
    26461 ccaatggaca aaccatcagg caatactcga aaaacaaatc acggaagaaa aagaagctct
    26521 aacggaactt caggaaaaac aaaagcaact agaaaattcc tactatcgtt ttgaaagctt
    26581 gaaaaaaagt tatgaagaaa aaacgatatt aataaacaag caaaaggaat tagctgctca
    26641 aaaagacgaa atagaggaaa agaaacgatg gattgggtat tatgaattta cgcaacgatt
    26701 agtcgaacca atccagcgcc tacaagaaat aaaagttgaa aaaatagaac tgtcacaaaa
    26761 aagtagtgag caaacaaaga aactagcgat gactacgcag aattatgaag agtggcaaaa
    26821 aaatgaagca aaccgcgtac gccgacaaac aaaaatcaaa gaagctgctc aaaaactgca
    26881 aaacaaacag atgatggtgc caattgtcga atccttacgt agtaaaaaac aagacgcaga
    26941 acaattaaca atttcattgg ataaagaaga attgaatgcc gagaagctgt atcaaaatct
    27001 cgctatctat caggaaagaa ctcgaattct tcaagaaaat ttactggatc aggaaaaact
    27061 gcaagaagat cgtcttgctt ttggacagtt aaaatatcta gatgaaaagc agcaagaaat
    27121 agaaaaacaa aaagcaaaag acactgggtc gctcgcacag ataagagaag aactcaaaaa
    27181 agcaactgaa gaaataacaa aaaatagtca gctattgaaa gaacagacag aacaagcaga
    27241 acagctgaaa agccaatggg caaagcagca aattgctcgt cttcagcttt ttttgcgtcc
    27301 aggggagcca tgtcttgttt gtggatcaac ggaacacccc atacaacaat taaaacacga
    27361 acagccaaat aaggaagata tcttaaaagt agaacaacaa ctggcagaag ctgagtctgc
    27421 tgtagaaaga actcagcaaa tcattgccaa aagtgaagca catgaaaata atcttcaaca
    27481 acaattatca gatttagaac aaaaaatcat ggcaacgaat atacaattgg ctgaacaaga
    27541 agaacacttg caacaggctt tgaaaaaggc atttcctgag ataacagaac aaaaaacaaa
    27601 aacaactctt gtagtaatcg aacaatcttt agatagacga aaaagcaaga tggccaatga
    27661 aagaatagag ctggaagaaa taagacaaca agcagaaaag ctcgaacaac aattagctgt
    27721 ctacaaacag cagcagacag aaaaaaataa tcagctccag caactgcaag gagaaatcaa
    27781 aagtttagaa caacaattgc aagatacgga tagtaactgt ttagaagagg agatacgacg
    27841 acttgaaaaa cagttagaag aagactcgaa tcaactagct gttgagcaga aagcaggagt
    27901 gcaattgttg caagatcttc atacgcttcg gacacagctt gatcttcaac atgaacaaat
    27961 agaaaaaata gatgaaaaaa tagaaaagga acaagaaaga atagctaaaa agttagaaag
    28021 ccagtcgtat ttttcaacaa ttgaagaaat ctgtgcattt gcacaacagc aagcacaata
    28081 taaagaaaac caacgtacag ttacttctta tcatgaagag caattagtga cagctgaccg
    28141 gctgcgtcaa ttagaaaata tccagctaac tgagacattc ccagatacag aagagatcag
    28201 ccaacagtta gaagaaataa gacgtatgat cgaaataaag caagaaaaga tatacggttt
    28261 acaagaacaa aaaattagta atcaaaaaat ctacgatgaa tgtctttcga tttatcacac
    28321 aagccagacg aaattagatg agcttacgca attacagcag ctttcgcaaa cattcaacgg
    28381 agaaaatcct aagaagacta gtttggaacg gtatgtgctt caggtttatc ttcaagaagt
    28441 gttacaagta gcaaatgatc atctccaacg tttaacgaaa aatcgttatc aatttgagct
    28501 agcggatacc attggcagtt atcgagggaa aaccggatta gaaatcaata tctatgatga
    28561 tcaggcaggt atgtctcgaa gtgcgcatac cctgtctggc ggtgagagtt ttattgcagc
    28621 actttcttta gcattagctc ttgccgatgt gatccagaca caagctggtg gtgtagcaat
    28681 cgatgcattg tttattgatg aaggatttgg gtcattagat gaagaagctt tggaaatggc
    28741 aatggaagct ttagaaacga tcgaaagtga aggaagaatg atcggcatca ttagtcatgt
    28801 aagagaattg aaagaacgag tattacaaca aataagaatt gatacagaag gtagcggaca
    28861 aagcaaaata cgctatcaac ttggataaaa agggagattg gtaactatga aatggagtat
    28921 tccagaaaaa gtcattgcac gtggacgtca atacttgcaa gaaggccgag tgttatcggt
    28981 tgtccctgac caagaagaaa acgtgtggca tgcagaagtc ttgggcagtg aattgtatat
    29041 ggttgatttg gatggtacag caaaagagat agattactgc caatgtccct attgggagga
    29101 acataaatat tgtaagcata cagttgctgt ggaactttat ttacggtcga aaaatctatc
    29161 tcgtatcatg aaaaaagacc agccaatgct agaaccggta aaagcaacaa gtgagggaga
    29221 aatcattgca aaaggttttt ctcgtctaaa aacacaaaac aatcctcaag cggtgcaacc
    29281 tttgattatt gaataccaag tagaaacgat tgagactaat cagtatcacc cagagctttc
    29341 tattttggca ctttacttgc gtatcggttc attggcatca ccgaaaaaaa catatatcgt
    29401 aaaaaacatc tatgattttt tacaagctta cacagaaaaa gaaacttaca ctgttaataa
    29461 acagtaccag tttcgtctag ataaacgggc gtttcaaaaa gaagatcaaa aaatactcac
    29521 gcatcttgca ggaagtgctc agacgcatca attattaggt acgaacggct tacaagtcaa
    29581 aggaaaacta gataaaagat atctgcttct cccagtagaa caagcccgct tcctgttaga
    29641 gcagatgaat cagactacac gtttgcatct gcagataaat gaacaaaacg atcaaggaat
    29701 acaattttct gatcaaaaca aacctttatc attcaaagtg gaaaaatttg gagaagatta
    29761 tttgctgcaa gcattgaatg attttgattt tttctttatg cattatcagt ggggagttat
    29821 ccaagggaca ttttattctc tgacgaaata tcaacaaacg gtttatcaaa cttggaaaca
    29881 attgattcgc cgattggaga aaccagaagt agtgttcaag aaaaaagaat taccgtcttt
    29941 gtttaaagag attatccctc tgctgtcaga gatcggcgac gtagctattg cccctgcagt
    30001 agaagaggaa gtagcagata tccccgtcga atttattttc tttttccgaa aagcaaaagg
    30061 aaaaatccaa gctcgagtcg atttcgtcta tggtgaagtc atctattcga ctgacccgaa
    30121 gcatgaggtc tcgacagaac aggcttttca ggtgttacgt gacacagtag aagagaaacg
    30181 agtacttgat ctattcagaa tgtatcgcta ccagaaaaat gaaactggtt atgaacgtcc
    30241 attgccaagc ggggaagaac tatatgcctt tttccgcaca gaactatctg tcttccgtca
    30301 gtttggtgaa gtcagacttg gaagaaaatt gcgagagttg tatttagatg cgcataaaca
    30361 tcaaccgaaa atcgaagtaa ccgagaccgg ttcatggctc gatattcgat tcgatgtgac
    30421 agggatagag gagcaagaaa tcgatcatgt cctgcaaagc ttgctgagaa atgatgcttt
    30481 ttatacatta gaaaatggtc aggttctttc ttttgattca gaagaatttc aacaaacaag
    30541 ccaagtgttg caacaattac gggaatcgat acgtacagaa gaaggaacga tccatgtacc
    30601 taaaaatcaa ggattgatca ttcaaaatca attggaaaaa agcaatgcta cttttagtga
    30661 atcgttccaa acgatggtac aagatctcat tcatccagaa agataccagg cacagcttcc
    30721 caaaggattg aatgcaacga tgagagatta tcaaaagcaa ggttttcgat ggttgaagat
    30781 gttaggtcat tatcagtttg gcggtatttt agccgatgaa atgggacttg ggaaaacact
    30841 acagacaatt gcctttttac tttcggaaaa agaagaacgg aaaagcttct ctgctttgat
    30901 cgtagcgcct gcgagcttaa tttacaactg gcaggcagag gtgagaaaat tcgccccttc
    30961 tttaagtatc caagtaatca atgggaataa aaaagaacgt gaagaattac tggctaaaga
    31021 cacagatatc cgagtgactt cttatgccag cttaagacag gatctagcta attatcagtc
    31081 gcaaaaaatt gattatttga ttcttgatga ggcacaaatg gtcaaaaata gcagtacgaa
    31141 aactgcacaa gcattgagag aattagctgt acctcagcgg tttgcactga gtgggacacc
    31201 aatcgagaat aatttagaag aattgtggtc attgtttgca acgattatgc caggattttt
    31261 cccgactaaa acaaaattca gggaacttgc cgcagaagaa attgcgcaaa tgattcgacc
    31321 gtttatttta cggcgagata aacaaactgt gttgaaagac ttgccggaaa aaacagagat
    31381 gaatctatat tctgcactta cagaagaaca aaaaactgtt tatttagctt acttgcgtca
    31441 aatgcgtgaa gaaatcacat caatggattc tgaagcattc aagaaaaacc ggatcgggat
    31501 acttgctggt ctgacaagac tgcgacaaat ttgctgcgat cctcgtctgt ttattgaaga
    31561 ttaccaaggc ggttccggca agctcgaaca agtaaaagat ctactggtag ctgccaaaga
    31621 gaacaaacga agagttttgc tgttttctca atttacaggg atgctgacta tcttgcaaga
    31681 agagttagcc gagctaggga tctcgacatt ttatttaaga ggcagtacga aaccacaaga
    31741 tcggttaagt atggtagacg catttaatgc tggcgaaaaa gatgtttttc tgatttcctt
    31801 aaaggctggg ggaactggat tgaatctcac gggagcagat acggtcatct tgtatgacct
    31861 ttggtggaat cctgccattg aagaacaagc tgctggacgt gcacatcgga tcggacaaaa
    31921 aaatgtggta gaagtgtggc gtatgatctc tgaaggaacg attgaggaac gcatggattc
    31981 actccagcaa gaaaaacgcg aactgttcca aaaagtgatc caaggtaatg aagaacagtt
    32041 gacgaaactg actgaagaag acatccgcat gattttaagt gtaggagaaa tcgaggagta
    32101 agagactcga aaaagcgttc agtaatgggt aagaaagcct ttctaaagaa gcgattgttt
    32161 atttgattta aaaaatagca aggaggctgg gattccattt tatcccagct tccttgctat
    32221 cattttttta tttctatttt taaaggttat acaaagattg tcttcattgt ctctaacagc
    32281 tgatcaaaag aagaaatctc gatatcgcaa attggagtct gttcaatttt gcgttctcga
    32341 tggttgaacc aaagcgcttg ccagttagca ctttttgcac ctagaacatc attgtcgtaa
    32401 ttatcgccaa cgtataaggt ctcttctgga agcatatgaa attctttctc agccagctga
    32461 aaaatctctt tttctggttt ttggaaacct gttgcttgag aaatgagcat attgcgggaa
    32521 ggaatccaat tgttcaattg aagttgcttc agtttttttg tctgatggtc agtcggccca
    32581 tttgtgataa ttcctaatgg gacattcttt tccttcaaga aatcgagggt ttttttgacc
    32641 tcctcatgca agcagatatt atcaagttct tcctcgtaaa ttttttgaaa taagaggcca
    32701 tcttcttccg taatatgagg gtagtctaaa tcttttagtg attggctgat ccgatgtgct
    32761 cgcatatatt ctagtgtcca atctcctgcc attacttttg ggaagttttc atcactatga
    32821 tggcggaacc ggatatatag tggatgcata tctgcatctg aaacaagcgg aacgactctt
    32881 ttgaccgcgt ttcgaaatgg ctgctgctga tcatacatcg tatcatcaac atcaaacaca
    32941 actgatttaa tcaaaatttt acacgccttt cggttttcat tgattttttc acaaatcctt
    33001 gactattgta acagaaaaaa ttgtctaaaa aagctgataa ataaaggttt ttcttactat
    33061 atttctattt ccttgacaga ataaggtttt tatatttcag tttttttcac taaaaatcgt
    33121 gaaacacgtc aa
//
LOCUS       DAZULY010000006        32504 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000006 DAZULY010000000
VERSION     DAZULY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.006"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..380)
                     /gene="rplL"
                     /locus_tag="ACG81A_000172"
     CDS             complement(12..380)
                     /gene="rplL"
                     /locus_tag="ACG81A_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFS5013580.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(433..936)
                     /gene="rplJ"
                     /locus_tag="ACG81A_000173"
     CDS             complement(433..936)
                     /gene="rplJ"
                     /locus_tag="ACG81A_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFS5013581.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(1140..1267)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(1346..2035)
                     /gene="rplA"
                     /locus_tag="ACG81A_000174"
     CDS             complement(1346..2035)
                     /gene="rplA"
                     /locus_tag="ACG81A_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFS5013582.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(2139..2561)
                     /gene="rplK"
                     /locus_tag="ACG81A_000175"
     CDS             complement(2139..2561)
                     /gene="rplK"
                     /locus_tag="ACG81A_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFS5013583.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            2904..3572
                     /gene="sdaAB"
                     /locus_tag="ACG81A_000176"
     CDS             2904..3572
                     /gene="sdaAB"
                     /locus_tag="ACG81A_000176"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFS5013584.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            3585..4457
                     /gene="sdaAA"
                     /locus_tag="ACG81A_000177"
     CDS             3585..4457
                     /gene="sdaAA"
                     /locus_tag="ACG81A_000177"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFS5013585.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(4548..5648)
                     /locus_tag="ACG81A_000178"
                     /pseudo
     CDS             complement(4548..5648)
                     /locus_tag="ACG81A_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(5740..6627)
                     /locus_tag="ACG81A_000179"
     CDS             complement(5740..6627)
                     /locus_tag="ACG81A_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5013586.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(6704..7249)
                     /gene="nusG"
                     /locus_tag="ACG81A_000180"
     CDS             complement(6704..7249)
                     /gene="nusG"
                     /locus_tag="ACG81A_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFS5013587.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(7350..7520)
                     /gene="secE"
                     /locus_tag="ACG81A_000181"
     CDS             complement(7350..7520)
                     /gene="secE"
                     /locus_tag="ACG81A_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFS5013588.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(7544..7696)
                     /gene="rpmG"
                     /locus_tag="ACG81A_000182"
     CDS             complement(7544..7696)
                     /gene="rpmG"
                     /locus_tag="ACG81A_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS5013589.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            7858..8502
                     /gene="cbpA"
                     /locus_tag="ACG81A_000183"
     CDS             7858..8502
                     /gene="cbpA"
                     /locus_tag="ACG81A_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFS5013590.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(8558..9061)
                     /locus_tag="ACG81A_000184"
     CDS             complement(8558..9061)
                     /locus_tag="ACG81A_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS5013591.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(9074..9120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            9658..10623
                     /locus_tag="ACG81A_000185"
     CDS             9658..10623
                     /locus_tag="ACG81A_000185"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5013592.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(10722..11894)
                     /locus_tag="ACG81A_000186"
     CDS             complement(10722..11894)
                     /locus_tag="ACG81A_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5013593.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(11881..12006)
                     /locus_tag="ACG81A_000187"
                     /pseudo
     CDS             complement(11881..12006)
                     /locus_tag="ACG81A_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            12114..13193
                     /locus_tag="ACG81A_000188"
     CDS             12114..13193
                     /locus_tag="ACG81A_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5013594.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
     gene            complement(13390..14091)
                     /locus_tag="ACG81A_000189"
                     /pseudo
     CDS             complement(13390..14091)
                     /locus_tag="ACG81A_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(14105..15029)
                     /locus_tag="ACG81A_000190"
                     /pseudo
     CDS             complement(14105..15029)
                     /locus_tag="ACG81A_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            15146..16225
                     /locus_tag="ACG81A_000191"
     CDS             15146..16225
                     /locus_tag="ACG81A_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS5013595.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            16222..16850
                     /locus_tag="ACG81A_000192"
                     /pseudo
     CDS             16222..16850
                     /locus_tag="ACG81A_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(16960..17187)
                     /locus_tag="ACG81A_000193"
     CDS             complement(16960..17187)
                     /locus_tag="ACG81A_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5013596.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(17290..19878)
                     /locus_tag="ACG81A_000194"
     CDS             complement(17290..19878)
                     /locus_tag="ACG81A_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFS5013597.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(19898..20557)
                     /locus_tag="ACG81A_000195"
     CDS             complement(19898..20557)
                     /locus_tag="ACG81A_000195"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5013598.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(20606..21109)
                     /gene="trmL"
                     /locus_tag="ACG81A_000196"
     CDS             complement(20606..21109)
                     /gene="trmL"
                     /locus_tag="ACG81A_000196"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFS5013599.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(21330..22196)
                     /locus_tag="ACG81A_000197"
     CDS             complement(21330..22196)
                     /locus_tag="ACG81A_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS5013600.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            22305..22946
                     /locus_tag="ACG81A_000198"
     CDS             22305..22946
                     /locus_tag="ACG81A_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFS5013601.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23008..24369)
                     /gene="mgtE"
                     /locus_tag="ACG81A_000199"
     CDS             complement(23008..24369)
                     /gene="mgtE"
                     /locus_tag="ACG81A_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFS5013602.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(24387..25286)
                     /locus_tag="ACG81A_000200"
     CDS             complement(24387..25286)
                     /locus_tag="ACG81A_000200"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS5013603.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(25288..26085)
                     /locus_tag="ACG81A_000201"
     CDS             complement(25288..26085)
                     /locus_tag="ACG81A_000201"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFS5013604.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26063..26767)
                     /locus_tag="ACG81A_000202"
     CDS             complement(26063..26767)
                     /locus_tag="ACG81A_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFS5013605.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            26898..27473
                     /locus_tag="ACG81A_000203"
     CDS             26898..27473
                     /locus_tag="ACG81A_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFS5013606.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            27591..28268
                     /locus_tag="ACG81A_000204"
     CDS             27591..28268
                     /locus_tag="ACG81A_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFS5013607.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(28347..30155)
                     /gene="pepF"
                     /locus_tag="ACG81A_000205"
     CDS             complement(28347..30155)
                     /gene="pepF"
                     /locus_tag="ACG81A_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFS5013608.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(30331..31329)
                     /locus_tag="ACG81A_000206"
     CDS             complement(30331..31329)
                     /locus_tag="ACG81A_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFS5013609.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(31555..32208)
                     /locus_tag="ACG81A_000207"
     CDS             complement(31555..32208)
                     /locus_tag="ACG81A_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFS5013610.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         9461 a         6877 c         5851 g        10315 t
ORIGIN      
        1 aatagtaaaa attatttaac agttacagat gcgccaactt cttcaagttt agctttcaat
       61 tcttcagctt cttctttaga tacgccttct ttaactggtg caggagcgcc atcaactaca
      121 gctttagctt ctttcaagcc taaaccagtt gcttcacgaa ctgctttgat aactttaact
      181 ttttggtctc cagctgcagt taattctaca gtgaattctg tttgttcttc agcagcagca
      241 gcgccaccag cagcagctac agctacagga gcagcagcag atacgccaaa ttcttcttca
      301 atagctttaa ctaggtcgtt aagttctagg atagttgctt cttttaactc agcaacgatg
      361 ttttcaatgt tcaatgccat tataatttcc tccgtttaat tgttcttttt tatttaatgc
      421 tttatgcggc aattaagctg catctccatc ttctttgtct gccactgctt tgacagcgta
      481 agccacgttg cggactggcg cttgtagtac agatagcaac atagatagaa gtccttcgcg
      541 atttggcaat tttgccaatg ctgtgatttc ttccaatgaa gagactttac cttcgatgat
      601 accgcctttg atttctaatg cttttgcttc tttagcaaat tcgtccatga ttttcgctgg
      661 tgcgactaca tcttcgttac tgaatgcaac agctgtaggt ccagtgaata cttcgtccat
      721 gccttcaagg ccagcttttt tagctgcacg tgaaagaata gaatttttga taactttcat
      781 ttctacgtta gcatcacgaa gttgtttacg taaacgtgtt acttcatcaa ctgttaatcc
      841 gcggtagtca acgactacta ctgatgcagc tgctgtaaat ttttcgctta cttcatcaac
      901 gattgctgct ttttttgcga taattgcttc actcattttt gagtttcacc tcctgaattt
      961 tgatggaaga gttttttgca ctgaaaaggc aagtcacttt tatagttact ttgctcaacc
     1021 agtattattc atcacctatt cacttcgttc atagtgattc tactcgcttt gctcgaaagg
     1081 aaactccttt cgctttgctc aaccagtatt gttcatcatc tcttcacttc gttcatagtg
     1141 attcacaata aaaaactcta tgccaccgca gacatagagg gactattgaa tatattcaat
     1201 aaacgtcctc ggtaggaaat taaggcttga tgccacctac tgtcttcggt acaagttaat
     1261 tattaacctc aactagctta gcatgctaaa ctagttgagt caagaattat ttttcagttg
     1321 gatcaatcaa cattctaata tttaattaga aagaagcttg gtcaacatgg ataccaggtc
     1381 caaatgttgt tgtgatcgtt acatttttca tgtattgacc tttagctgct gatggtttag
     1441 cttttagcaa tacatcatgg attgctgcaa agttttcaac taatttttca tcgtcaaatg
     1501 aaacttttcc gattggtaca tggatatttc cagctttgtc cacacggtaa gttactttac
     1561 cagctttcac ttcgtttact gcttttgtta cgtccattgt aactgtacca gttttagggt
     1621 ttggcattaa gccttttggt cctaatactc gtcctagacg acctacagta gccatcatat
     1681 caggtgttgc aacaactaca tcaaagtcaa accatccgtt ttggattttt tgaaccatgt
     1741 cgtcgtcacc tacgaaatct gcaccagctg cttcagcttc ttttgctttg tctcctttag
     1801 caaaaactaa aacagtttgt gttttaccag ttccgtttgg taatacaaca gcaccacgga
     1861 tttgttggtc tgctttttta gggtctacgt tcaaacggta tgcaacttca actgttgcat
     1921 caaattttgc aatgtttgtt tcttttgcta aagctactgc ttcggcaaca ggataaactt
     1981 tacttgaatc aacttttttt aatgcatctt gcattttttt gctcttttta gccattttgg
     2041 ttcctccttg attgtggtta taacggttgt acctcccacg tgttctatat ctttcaatat
     2101 agagccaact gagaagctgc tgtcttaggg tgtatagatt attctacagt gatccccatg
     2161 cttcgtgcag ttccttcaac catgcgcatt gctgcttcaa cgttagctgc gtttaggtct
     2221 tccattttta attcagcgat ttctcttact tgatcgctag aaactttcgc aactttattt
     2281 ttgtttggtt cgcctgaacc tttttcgatt ttagctgctt ttttcaatag aacagcagct
     2341 ggcggtgttt ttgtaacgaa tgtgaatgaa cggtcttcgt atacagaaat aacaaccgga
     2401 atgatcaaac ctgcttgatc agctgtacga gcgttgaatt cttttgtgaa tcccatgata
     2461 ttgatacccg cttgacctag tgcaggacct actggtggag ctggagttgc tttacctgca
     2521 ggaatttgca atttaacgat tttttctact ttctttgcca cgagacatac ctccttaagt
     2581 ccgtgatgtg gttaattgga gatgcatatt tctcctccca cgttttatct gatctgaaaa
     2641 aatccagaca aataatgtgc aaaacaatgc acactggtac agtatagcaa atttatttgt
     2701 tttttcaaga tttttttctg ctatttttac attttgcttg aataaacgct aaatttattc
     2761 taaaaaaaca acaatttttc gttctcattt cttcttttat gttacacttg cgctagcatg
     2821 gttcaacaga tgaaagcaaa caataacacg gttttttaga cccttgtaca aagattccta
     2881 cataacaaaa tgaggtggag aaaatgaaag atttaaggta tagaagtgtc tttgatatta
     2941 ttggacctgt gatgattggt ccaagtagct ctcacactgc cggagctgct agaatcggga
     3001 aaatcatacg tagtatattt ggcgaacagc ctgattctgt agatatttat ctatatgaat
     3061 catttgcaaa aacctatcgg gggcacggaa cagatattgc attagttggc ggcctgttag
     3121 ggatggaacc cgatgatgaa cgtttagctg attctttgaa attggcatat gaagccggta
     3181 tggaagtctt gtttgttcct aagagtgaaa aagccgaaca tccaaactct gtaaagatgc
     3241 ttctaaaaaa aggaacacga aagctttcag tcactggcat ttcgatcggc ggtggcaata
     3301 tccagatttc tgaattgaat ggattcaaaa tttctttaag catgggtact cctaccttga
     3361 tcgttgtcca ccaagatgtt ccaggtatga ttgctaaagt gactagtata ctttctgaga
     3421 ccaatacgaa tatcggcact atgacagtga cacgggaatc aaaaggtgaa aatgccatta
     3481 tgataattga agtagatgat cctcaagtag aagaaacggt aaaaagactc aaacagctgc
     3541 caaatatcga cagcgtaaat tatttcgatt aaggagctaa aaaaatgttt ttatcgattg
     3601 aagaattagt agatcaagca aaagcttatc caagtgtagc agaattgatg attgcagttg
     3661 aaatggaaat gagtggccgt tcccgtagtc acatcatcga gatcatggaa aaaaacctct
     3721 cagtaatgga acaatcaatc aacgagggaa aagaaggtgt tacctccgtc acaggaatta
     3781 ccggtggtga tgcaaaaaaa ttagatgatt atttaaatga ggcagacttt ttaagtggag
     3841 aaacaatctt aaaagctgta cgcaatgctg tagctgttaa tgaagtcaat gcaaaaatgg
     3901 ggttgatttg tgctacaccg actgccggaa gcgcgggtgt tgttcctggt gtgctgatgg
     3961 ctgtccgaga tcggcttgat ttgacacatg aacagcaatt ggaattctta tttaccgctg
     4021 gcgctttcgg gttagtcatc gcaaacaacg cttctatcag tggtgcagaa gggggatgtc
     4081 aagcagaagt tggttctgca agtgcaatgg cagcagcagc tttggtttgt gcaaaaaatg
     4141 ggactcctca tcaagcagca caagcagtag ccattacatt gaaaaatatg atgggactga
     4201 tttgcgatcc cgttgcaggc ttagttgaag tcccgtgtgt caaacgtaat gctttaggct
     4261 cttctcaagc ttttatctct gctgatatgg cattagcggg tatcgaaagt gtcattccgc
     4321 cggatgaagt cgttgctgca atgtaccaag tcggacgtca aatgccgtcg attttcaaag
     4381 aaacagcaga aggtggctta gctgttacgc caactgctaa acgacttgcg gaagagattt
     4441 taaataagag ggcgtgataa agctccttaa tttcgagtat taagagatta gttatcttca
     4501 taacaaaaaa cgaagagagg actaattgcc cttctcttcg ttttttatta atccatcaat
     4561 ttcgtatttt ttacttcatc gatcgttttt gttcctgcaa gttgcatcgt gatcgacaat
     4621 tctttgttca ggtgttcaaa aacagatgtt actccttctg ccccacctag attcaaccca
     4681 taaatcactg gtcggccaat cgcaactaag tcagcaccac ttgccagagc tttgaagaca
     4741 tgctctcctc gacggacacc agagtcgaaa atcactggta cacgtttgtt gactacttcg
     4801 gcaatttttg gcaatacttc aaaggacgca ggacctccat ctagttgacg tccaccatga
     4861 ttggataccc agataccatc tgcccctgca gagatagcga ttactgcatc ttctggcgat
     4921 tgtattcctt ttacgataac tggaagatgg gtgatttctt tgattgtttt gatgtcatct
     4981 ggtgtcaagc cttgttttgc agctgcataa atttctgcga ttccttttcc ttcgccatta
     5041 ccacttgcac tttgttcgct atacgcagct aagttaggca ttggtaatgg aaattggaat
     5101 tgattgatga cgtcttcttc acggtaacca ccgagcgttg aatctgccgt taaaatgata
     5161 gctttagccc ctgcttctac tgctttgttc aaaatgaact cattgaatct gtcgtcttta
     5221 ctcatatata attggaaaaa ctgcggtgcg cctggcgctg catcggctgc gtctttgatt
     5281 gtcgtatttg cataagtgct gatggaaaag attgatccag cagcagctac tcctttagct
     5341 gtatcagctt cccctttttc atgtgccaat ccttgggccg ctgatggcgc ttggataatc
     5401 ggtgtatcta attcaattcc gaaaacagat gtatgcaaat cggctgagtc gattccgcgt
     5461 aatatccgtg gcatgatttt ctttgtatta aaagaagtcg tattttcttt catcgtccac
     5521 tcatcttcag aaccaccgcg aatatagcca aaagctcctg cttccatgcg ctcttttaca
     5581 cgaccttcta gttaggcaat gtttacaata tcgattggat gttcttcatt acttgtatga
     5641 taagtcatag tagatccttc tttcatcttc ttgattacat ataaatcata ctaaacttac
     5701 aaaaagtaag caaacattta ctctcagata cttttatcgc tattctttcc aaataaatcg
     5761 acttacaagc tgatctactt cttggttttc atgaagcata ctatgagagg cattttcacc
     5821 tgtcactact ttttcctcta tttcatttcc gcgttgcttc aacaacgcat agaccgctaa
     5881 tgcactgttt aacggtactg ttccatcact caagtctgtt tcatctagtt gaccagcaat
     5941 caagaaaaat cgagtggtta taggaatatt accgatcatt tgctggtaat cttgatagcg
     6001 gttagaaacg acagcaggcc cctttgctaa ctcatctgtc agagactgcg cactatcatc
     6061 gacaaaatca ttaaaaggcg cgccaattgc tgttaatgtc tgaacttgtg gcaatgacgt
     6121 atcctgtcca tacgttccta aatagcgtag tgcactaact cctcccatgg aatgccccac
     6181 tatgttgact ttatctatgc cgtagttttc tttcagatac aaaagacaag ttttgatcca
     6241 ttctgcttga ttccactcgt tatttttatt atcttcaaac aatacttgga ccatcggatt
     6301 gtctgctttt ccagacaaat ctcctgttgc ttgtatttgt ccatctgttc ctactgttaa
     6361 aaccaattct tttactgctt gctgattttt ttccaaacgc cgcaacatcc ctttaaagga
     6421 attttctgtc ccgctgtagc cgtggaaaaa aagtgttgga atagtggttt tttctatcac
     6481 tttactggat tctgccgttt tttctttatc agaaacttca gtagtattta tcttcgtcgt
     6541 ctggcaacca cttaacgcaa acaaactgat aaggactaaa gctgctgcag ttgcacgttt
     6601 gcataaattt ttgattggtt ctgtcataca cctcttcctt tccatataga aaaaagaaag
     6661 acttgaaagg ccaatcatat gatcgacatt tcaagtctct ttattaatcg attttatcta
     6721 cttgatcgaa atcaagttct gtactggttt cacgtccaaa catatcgatg ttgactttca
     6781 atttttcttt ttctccgtca acttctgtta cttgtccttc taatccagca aatgcgcctt
     6841 cgatgatttt cacgacttcg ccaacttcta cttctagatc attttgtcgc aggctcatac
     6901 cgatagaacg caagatatga ttgacttctt ctggaagtaa tggtgctggt ttgcttccgg
     6961 ctccgtgtga accaacgaat cctgtcacac caggtgtgtt gcggacaaca taccatgaag
     7021 cgtctgtcat gatcatttca acaagtacgt atcctgggaa tgttttatga acgatttctt
     7081 tttctttccc atttttgact tcagtttctt tttcttcagg aacaacgaca cggaaaataa
     7141 agtcggccat tttcatactt tgcgcacgag attcgatatt tgcttttact ttgttttcat
     7201 atcctgaata tgtatgcaag acataccagt ttttttcaaa agattccatt gaaggctcct
     7261 catttcattg taaaataaaa aaacctcagt acacccgaag tttttctttc gcttcattat
     7321 agcactaaaa gtacgttgtg gctagcgagt tattttaaaa tccagccaaa taagctttgg
     7381 atggctgtat ccattacgta aaacatcaat ccgaaaataa tagaagtttc gatgactact
     7441 aacgtatctt tacggagttg ttttctgctt ggccaagtta cttgtttgat ttcatctttt
     7501 acgcttttta gaaatttcat gagacaacct cccttgctac tgtttatttc gtttctttat
     7561 gtaatgtata ttgattacaa tatttacaaa acttattgat ttccaaacgt tcgccgcttt
     7621 ttccttcact gactgatttt gtgtagttgc gagacccaca aacggaacaa gcgagtgctg
     7681 cttttttctt tgccatttac ttctgccccc ttgctgagaa tcagacttat acttcataac
     7741 agtatcacgt aactttttac ttgtcaattt gtgaaataaa ctatctattg ttttggataa
     7801 aattatgata tgattaattt gaaattaagc taaaatgaga caattggagg tatagcgatg
     7861 ctattaaaaa cacttgtata taagaaaaaa gatctgacaa ccgttacaga gactagtaca
     7921 ttagaagaag cattaaaaat tttagaaact tccggatacc gttgtgtgcc gatcttagat
     7981 tcttcaggta atattttccg tggaaacatt tacaaaatgc atatctatcg tcataaagca
     8041 aatggcggtg atatgacgct tcctgtcact catcttttga aaaacgctac aaagttcatt
     8101 tacctggata cttctttctt caaagtattc tttacgatca aagagcttcc ttatattgct
     8161 gtccttgaca gtgaaaatca tttttatggt attttgacac atagtacgtt gcttaatatg
     8221 ctctctcaat cttggagcgt agaacaagga agctatgtac tgacaattgc ttccacagga
     8281 aagcaaggag atttggctgc tattgcaaaa atcatttcaa aacacagcag cattgccagc
     8341 tgtattactc ttgatatcga aaaagacgaa tacattcgac gtacattgat cacgctaccg
     8401 gctaacactg aagcgaaagt atgtgaaacg atcgtcaacc acttagagaa aaagaattat
     8461 aaagtggtag aaattgaaaa tttgcaaaac caagaagcat aatcaaaaag cgaaaaaaga
     8521 aaacagctaa tctgctttct tttttcgctt tttataatta gacacgcttt tcaaaatcaa
     8581 gcgcgcgatc taaataatac atcaatggtg ctgaaacagc catgacgata aactcactta
     8641 atgcaagcgt gccataggtt gtccaaaatg cttggccacc tgttggaact aacatataag
     8701 caatcagtcc catgctgatg gtaaagaaga tcgtgttcaa tactaaacgc atctttacat
     8761 ttttcacttt gttttgcaat aaagctgtca ggcctaaaga aagcaatgtt tggccgccac
     8821 caaaaaccac atccatgata ccaaaaccaa agaaggcatt atagacgatg accccgccta
     8881 aaactcccca aagcaattta cggttaaaga caactaaatg attcaaactt tctgaaatac
     8941 gaaactggat cggccctgag gatacaggcg caactaaaat agttaacgct agataaagag
     9001 ccatgatgat gccatttaca acgattacgg ataaccgttg tctgcttttc gttttttgca
     9061 ttttctctcc tcttttctcc tagttttttt aacgcgggat ggttgatgaa ccgcggttat
     9121 gtgaaacaaa caataattat aacagacttt tcatttattt caatccgtct tatctgcttt
     9181 taaaaaagca aaagcttaaa caatgtaaac gtttgctaaa aatcgtggga tatggtataa
     9241 agtaagtgta aggggtcata agaattccca gtaaagcaga cgaagtagga tctgcaagaa
     9301 cagaattgtt gtgaaactta aataaataaa aaaggatgtg ggaatttgag caacctctta
     9361 ctagagaacg gtctagcaag cagctgaaga tctaagtgga catgccgaac cttggaactt
     9421 ctccttacgt aagtaatgga tcatgcgctt gctaggcttt tttgtgtttt tcggtcttcg
     9481 gacctttttc attttcctag acttctgcta taataaccaa gaattaattt tgtaacagat
     9541 ttttttgaaa aaacaagttt ctttcattca aaaaatagaa aaagcttgct aattattggt
     9601 taaccagcaa acaggagtat taaaatatct tgttttattg aatagcagga ggaaatcatg
     9661 aaattccatt atcatatatt gatcaatcca tcggcaggca gcggaaatgg ctataaagta
     9721 gcagaaagaa ttttaccggt attaaaaaat aagcatattg actatacact tcattatagt
     9781 gaatacaaag gacacgatgc tgagattgct gaaactttgg caaaagaaac attgattcct
     9841 tggatagaag aagaacagaa aacaatcgat actttccccc ttttgatcat cgtaggaggt
     9901 gacggcacct tacatcaggt acttgatact ttctatcaga tggaagtaga attcccagtt
     9961 gcttatattc cagcaggttc tggagatgat tttgctcgag gggcaggcct tccaaaaaat
    10021 cccaaaaaag gacttcagct gattttagca gctcaatcac cagaaaaagt ccacataatg
    10081 gcttatgaag aaaaaataag tgaaaaaaaa ggaatagctg ttaataattt cggtatagga
    10141 ttagatgcag ctatcgtcca tgcgacaaat cattcttcca caaaaaagcg attgaacaaa
    10201 tacaacttag gatcattttc gtatttattc tctattctcc gtgctttatt cactcaaaaa
    10261 ggatttccaa ttctggtaga tgcaggagga aaacaattaa gtttttcaaa cgcattttta
    10321 tgtacagcaa cgaagcatcc tttttttggc ggaggaatcg cgattgttcc aactgctgat
    10381 gcgtctaaac cagtcattga ttttgtcgtg gtcgaacgaa tcaatttctt taaaatcttg
    10441 tggctcattc ttttacttat acagaaaaaa caattgaaat cgaaatattt ccatcactac
    10501 acaacaaaca gattacgtat cgtttcaaca acaccgcaac atggtcaaga agatggagaa
    10561 gaaatggaaa aacgaccttt tgatatcaca ataaccacaa ctaaacagtt gttttggtgt
    10621 taataaaaga ggttttgtca gaagctacat ctggaacacc gtttattcgg aaaaaaggac
    10681 tgtgacacga cttttgtcac agtccttttt ttactatgca gttaattctt tgcccaattg
    10741 ggttgccatc gatagtttct ttctttaccc aaaataaatt ggatcattga aaacaccgca
    10801 gcaaaagcaa ctaagacaca accattgaac caaaaagatt ctgcaaatgt ttgattgtaa
    10861 ctgagtgttt cactgatcaa gttattattt tgaacaaacc aatatgttgg tgtgaatgcc
    10921 gctattttgc taaccatttc tggcaagtat tctgctggta caaacacccc accgataaag
    10981 caactgcctg tgataaaaat atctttgatc ccaccgctgg tttctgagtt ttttaccaat
    11041 gaagtgatac aagccgaaaa gctaatgatc ggcaataaaa acaagaaagt atttaaaata
    11101 tgataccata ccgttatatc ccacgaggtg tggatcacta acagaaccat tagaagtaag
    11161 aagccactga acaacagcag cgagtaacta atcaaactaa aggttacttt gcgattcaag
    11221 tttcgtttgg taatgggcgc actctggttt cgtttactga tctctttgcg attaaatgct
    11281 agattgatga cgctgaaacc gctaaaaatg gatagaaaca acccataaga caagaaatta
    11341 aaggttgtgc ccgtcaacag ctttttcatc cgctgactat atcccgcact tagctcaact
    11401 tctccagtaa ccgagagtgc catttgtgtc tgttccatag ctgcatcgat tgttttatct
    11461 tcttcttgaa acagaatcag tgtttgcaaa aaggtgttca cttgctgtgt aacaactgtt
    11521 ttagaaaagg catctgggcg actctgtatc ttgatcgtcg gcatctttcc tacttcgact
    11581 tgttcggtaa agtctgcagg cagatgtaag ataatgttta cacgattaaa ataaagagca
    11641 tcatcgattt ctttttgtga ggttccatcc aatgtaacga ctgtttcctt gtcattcaaa
    11701 tatttacgaa ggccatcgac tacaggcgac tcttcttcac taatcaaggc gattttcgca
    11761 tcttgcagag aggtattttc aacatcaata ttttgcgcaa aaaagaatgt ggcaacaaca
    11821 gtgataatta cacctatcag taaattcgat ttatttgctt taagcaatgt ccaaatcgtt
    11881 ttataaagcg tcataacgca cccctctttc catccaagca tttcctaacg caaaaaggaa
    11941 aataaatccg atcaaccata gaagattggt ataaaaacta gccaaatcag tgtagaaata
    12001 aagtttaggc ggattttata atgaacttag ccatttaaaa taaaaaaaac gccacgtgaa
    12061 ttccatgtta tattgaagtt accacaactc tcaatagaca aggatgaatt cacatgacgc
    12121 aaacccatga taacacagtt tcacgcaagg gaaaacatct ttcctactca gaacgttgcc
    12181 aaatcgctat cctgaaaaag gaaggctatt ccaaccgtca ggttgccaaa gtgctgggac
    12241 gtgttccgca aaccataaac aatgagattg atcgcggaac catcacccag ttaaaacgcc
    12301 aaacgcaaaa tggaaaagtg tatgacacct actattctgt ttatgacgct gatgcaggac
    12361 aggccttcta tgaaaagcaa cgactgaact gtggcagacg ccccaaatgg gcgaacacaa
    12421 atgccttcat tgattgggca gatgacaaaa tgttaaatga aaaatggtcg ccggacgtcg
    12481 tggtcggatt tgccagaaaa cacgagctgt tcgattgtgc catcattcct ggcacaaccg
    12541 ctctttatca atggattgac cgcggaatca tgcgaacaaa gaacatggac ttgcttgaaa
    12601 aactgtcccg taaaatcaaa gcggtccgcc caggacaccg tcccaacaag cgtgttctcg
    12661 ggacgtcaat tgaagaacgc cctaaagaag ttgaaacacg ggaaacgttc ggtcattggg
    12721 aaatcgacac ggtagtggga aacaagacca agtcggatgc cgtgctgctg acgctggctg
    12781 aacggcagac acgctttgaa gtcatcctaa agttgaacgg gaaggacacc gaatcggtgg
    12841 accaagcggt tcaacagcta agagaacggg ctggcgctaa cttcgatcaa ttatttaaga
    12901 ccgttacatc cgacaatggc tcggagtttt ctgggctaca cgatgctctg caagatgtag
    12961 ctgacgtcta tttcagccac ccctatgctt cttgggagcg tgggacgagc gaaaaccagc
    13021 acaaattaat tcgccgtttc gtccccaagg gaagcccgat tagtgaggtg agcgagaacc
    13081 agattttgcg gatccagcgt tggatgaacg attacccgcg gaaaattttg ggttatgcga
    13141 caccacatga tacattcgtc caggcattca agcaagaacg gttggtggct tgacccatta
    13201 ttccttgata acgatcctca ctcgggacat ggcttcatgt ctggaaagct ggcctggggc
    13261 cagaatcgtc tgaagactat catacctcag ttcattatca aggcctcact gatttagaat
    13321 aacgaggcag ttacggtatc aaacaaatga gttacctgta gtggctaact taaacttgaa
    13381 atttacggac gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg
    13441 aatcaaggac aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg
    13501 gaccccccac atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact
    13561 tttgaaactc atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac
    13621 agtcatgatg ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc
    13681 aatacgctgt aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg
    13741 acccacatgg agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc
    13801 atttagcttt tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc
    13861 attgacttcg acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc
    13921 tttgattaca attccagtat taatcgtttc gagattgcca ccttgatcaa tctcgccaaa
    13981 ggccgccata aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga
    14041 tttatttcgc agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc
    14101 tcctctagtc tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt
    14161 ctttggttgc tatttttccg taactgatct tttcttcttg taagattgta agaatcggca
    14221 acaatactgt atttagttca gtagttgtta cttggagctg acctttttta taagcgattc
    14281 catcagaagc tattgttcgc tgacaacacg ttttctggga gtaatgggat gtcgatcgtc
    14341 aaacgatccg ccgtccgtac catatctttt agagattctt tggttccttt agcaatcacc
    14401 tgtccttgat ccatgatcac gatctggtcg caaagaattt ctacttcttc catgtaatgt
    14461 gttgtataga caatggttgc tccttgtcga ttcagctcct tgatactttc taaaatttta
    14521 ttccgacttt gggggtctac agctactgtc ggctcatcta aaaaaattaa ccgcggcttg
    14581 tgggcaatcc cacatgcgat gttcaaccga cgtttcaagc caccactaag ttctcctgga
    14641 cgaaacgaaa catatttttc taatcccact aattgaatca cctcttcaat caaggctttc
    14701 cttttctctt tttcggaaac atataaaccg caaaaataat cgatattttc ttgcacactt
    14761 agttcgtcaa agaccgcaac gtcttgagga acgaccccaa tcattgcctt gacagcataa
    14821 ttttcaggag tcattgttgt cccgaacaat ttaatggttc ctttgtcata atttaacaaa
    14881 gaaagcatac agttgatggc agtcgatttc ccactgccgt ttggtcctaa cagacctaag
    14941 acttctcctt cttgtacttg cagatcaaaa tgattcaatg ctacaaaatc tccatagcgt
    15001 ttgactaatt tatcaatttc gataatcatc atgaaaaact ccttccaaat ttcttgttga
    15061 tctgactata ataaagtgca agaaaagaaa ctagtgacga ttggctaaac cacacatgac
    15121 atttgtcaca atgaaggagt ctgctatgca taaatctcgg ctactcaacg aaactagctt
    15181 tctactggtt atctcattgc ttttgctggt aagtcaagga ttcgttgcta tgcaagttat
    15241 ctatttattg atcgccgttt tattcactgg tgtcagttat ttactgccta caaagccaaa
    15301 ttggattttc caagttattc tatttctttt atgtgccttt ttacctgatc ttgtcctgtt
    15361 tctgccagcg acgatgcgag tgatcgcccc tgaagaaagc caccttgatt acttgttttt
    15421 gttaggtttt ttgttgattt cgacggttgg taatcatccc cccatcaagg tcgcctttgt
    15481 cgtaattttg ctgttttttt ccttttactt acgaaaaaaa gatcaacgaa cgaccgatct
    15541 tgagaaaaag tacattgtct ccaaagatac tcattgggaa aaacaacaag agttatcgga
    15601 acaaaataaa gcattggccc tgtcacaaga aacacaatta gccttgcaaa ttgtggaaga
    15661 acggaaccga attgcccgag acatccatga caatgtcggt catctgctgt caagtgcgat
    15721 tttacaaatt ggtgcgcttg aaatgatcaa tcaagaacca aaactaaaag aaccattaga
    15781 aaatttaagt gccaccgttc acactgggat ggatcggatt cgtcaaagtg tccatgacct
    15841 ccatgagaca tcgatctctt ttcaacactc cttagagttt ctgatcgctg attttccttc
    15901 ttctgttacg attgaaggaa acttgtttga aggattgaat gaaacgcagc aaaactgctt
    15961 cttaatggtc atcaaagaag ccttaaccaa cagtatcaaa catagtcatg ccaaaaccgt
    16021 tgttttacat tttcgaacgt tgccggcctt ttatcgcttg caaatcacga atgatggcac
    16081 cagcccacaa aaaaattctg ctcccggtat tggtttgaaa acgatgcgtc aacgtgttca
    16141 agaacaaaag ggacaagtcc atattttcca agaaaaccag caatttcagt taacgatcat
    16201 tttaccaagg gagggacacc aatgataaca gtagtcatta ttgagatgat ccatttgtga
    16261 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    16321 attgtgccaa ggatgcgcta gtcttatacg aaacccatcg tcccgatgtt ttgctgacgg
    16381 acatccgcat gcctaaacaa acgggcatcg atgctgccaa agaaattttg gctaaatttc
    16441 ccaaagccat ccttttgctt ttgacgacat tcaaagatga agaatacatc cgcgaagcat
    16501 tttccatcgg tgtcaaaggg tatttaatca aacagaactt gcaagcgatt attccttctg
    16561 tcaaatccgc ttataatgga caggccgtat ttggtaatga aatcatcgag actttcactc
    16621 aaatgatgaa gaataaaccg acgattgata gtcatgctta tgcttctttt agtgaacgtg
    16681 agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa taaagaaatt gcagatgcct
    16741 tgtacttgag tgacggcact gttcgcaatt acatcagcca attactagaa aagctagatt
    16801 tacgggatcg cacgcaattg gctatctatt tttatcagca tttgcaataa gtcttatcta
    16861 acacaaaaaa acctgaatga tgctccttcg catcattcag gtttttttca catcttctat
    16921 tcaattgtcc cgcaaaccat ttcctcacga ttgaaagaat caaggattcg cagggctgcc
    16981 aggattcgtt ggattgttcg gcgtgttagg cggaaaaata ggcgccttgt ctaacgtttt
    17041 ttctagtttt gattcttcac gatcgtctcg ttctacatca ggactttcat tcaatttgaa
    17101 ttctttttcc atctcatcta actttttaaa gtcattgcca gattcttttg ctaatttcgc
    17161 taatttatct tcggtttctt tagccataaa gtaacgcccc ccttttgatt tcagcttagc
    17221 atatcacact gaaaaaacaa aagaaaacgc taaccaacga tcatttttag ctatttctca
    17281 tctttaaaat caagcatttt ttgccggtat aaaatcgtag ggagaactgt tttccatacc
    17341 aatcatcgga tctactaact ggttatcgat cacttctgct gtaagaacga aatctttaga
    17401 aacagcaggt tctacctctt ctttcttttc ttctccaaca aatgaagaaa acagatctgg
    17461 ttccactgtt tccattactt ggtttttcgt ttcattcgtc ccactgtcat cttctacagg
    17521 tagcttttct tttacaaccc gtggctcttt tgtatagttc ccatcttcaa atggatcatt
    17581 tccgaccaaa gaatcttcat aagctgctag tttggtccgg agagtgatat tcattttatc
    17641 attatccatg atccttgatt tcaacgtagt catccggctt gctgattcat tcgctacaca
    17701 agtgacatag gcttgctctt cccctaacaa aatcaacagt tccttactgc gagtcactgc
    17761 cgtgtagagc aaattacgct gaagcatccg ctgatattga tggaccatgg gcaaaataac
    17821 catcttgaat tcactgcctt gtgatttatg gattgagcaa caataagaaa gagtgatttt
    17881 attccattcg tttcttttat aagtgacctc atttgcatca aactggataa ctaattcatc
    17941 cactttatct tcagaatctt ttgccaagat gatacccgtg atttctccca tatctccatt
    18001 gaatacattc aattcgggag tattgactaa ttggaggact ttgtctccta tccgataaac
    18061 ggtgtcgttc caatgtactt ccttttttcc aggtttccct ggattgaata tctcctgcat
    18121 cattttattt aacgcatcga tccctgcagg tccccgatac attggtgcta atacttggat
    18181 atcttgtgca cggaatccct tatttttcgc tttttccaca attttagaga tcaagggttc
    18241 aatttgataa gcatcacttc ggaaatagga acgatctttt tgattctttg tgaaatcttt
    18301 tggcagacgt ccttctttga tctcatgggc aagaggaata atactagaac catcaccttg
    18361 ccgataaatc tgattgagtt ccattttggg aatttgttcg atttccaaaa gatcatggag
    18421 tacttgacca ggaccgacag agggaagctg atctttgtct cctactaaga taacttgcat
    18481 attcgtcgga atcgctttga ataatgtgtt agccagccat gtatcgacca tagacatctc
    18541 atcaacaatc aaaagtccgc cttctaattc tttcgtactg actgaaggtg ttttttcccg
    18601 tccattcagc cccagaagac gatggatcgt actggcaggc agacctgtcg tttcgttcat
    18661 tcgtttagct gctcggccag ttggtgctgc aagtagaata ggaaaaattt cttgggtata
    18721 ttttgaagga tccaattcca gtccgttcaa ctctgcaaaa agattgacga ttccgttgat
    18781 cacagtagtt ttccccgtac ctggtcctcc tgttaaaata aatagagggg aacggattgc
    18841 ttcagcgata gcttgttctt gcgaaggtcc atactgtatg ccgaatattt tttcgatacg
    18901 ccgaatattc ttctgcactt ctttttcagg ataatggatt tcttttttac gactgagaag
    18961 gcgctggatc gaattaccga tcccccattc agaaaagtac aaactgtttt cgtaaagatt
    19021 agtctcttct tgttgtatct ttccttcttc aacaagttgg ataattacag tagcaacctg
    19081 atcaggctgg atttcaattg ggcgacttgt ttctaaaata cggatcgttt gctgaagcaa
    19141 tgtctccgcc ggtacgtatg tatctcctgt ttgaactgca tgctgaaaaa tctgatgcaa
    19201 gatagctgct cgaattcttc tcggtgaatc tgctgcaata ccgatttgtt cggctagatt
    19261 atctgcacgt ttgaacccga ttccttctat gtcttcgact agctggtacg gattttcttc
    19321 gatgatatcc aaagactcgt tcttataggc ctggtagata ctgaaagcta actgactacc
    19381 gaacccgtaa cgattcagtc ccatgatcac ttgatccatc ccgtggttta gccgaatcgt
    19441 ctcagcaagc atttctcttt ttggcttcgt caaaccggag atttgtttta ataccgaagg
    19501 atcagcaatg attttttcga tagcatcttc tcccagcaat tctacaattt tttctgctgt
    19561 tttcttcccg attcctggga atttttcgct ggaaagatac gctacgatcc catttgcagt
    19621 tgttggcttt tcttgctcat atctttcgag tttaagctgc cttccgtatt taggatgatc
    19681 cactaattct ccataaaaac gataagtctc attttcttga atatcgccga aacttccggt
    19741 gactacaatc tctttttcac ggtaatcagc tgtggtttca ctgatgtgta ccaaaagcac
    19801 tttgtaaaaa ttactaggat tttgaaaaaa aatcgcggca actgtaccaa cgaaataagg
    19861 catttcttct ggctgcatcg gaagccgcct ccttcttcta tagtaaaggt tctggtgctg
    19921 tttcagttcc taaaaaatct gtttggttcc atactttcag ttcatatggc aataatctgc
    19981 aaggctcttc agtctctaaa atcgtcaaac tactattgaa caatccaccc atttcacgca
    20041 actcacttag ctcttttcct gtcatccatt gaatggctgc agttaaagaa gctccatgtc
    20101 ctacgaataa aagtgggcca tctttatgtt gggcaaccgc atctgctaca acagtttcga
    20161 tacgtgtgat tgccgattca atcgtttctc cgtgaaaagg agaaggatca tacagatcta
    20221 aatgattacg caaattcggc agattatccg gatatttctg gtacatttca tcgatcgact
    20281 gaccttctaa agtacccagc cctagttctc ttagcttatc tgtataaaaa atctctaccg
    20341 gacgagcaag ctctttgttg ataccttctg ctgtatcacg tgctcgcttt gctgtactgg
    20401 aataaattgc tttaaaagga acatatttta cctttttccc aaatgctttg atttcttcgt
    20461 aactttgtgg caaaagcgga gaatcgccat tgctcccttg aaaacgtctt tcctgattcc
    20521 attgtgtctt cccatgtcgg gtaaaatata gttccatacg cagcaaactc ctgtctttct
    20581 tttattttac tctataataa actgtctaat ccagcttgtc attttcatag tgatgcgtca
    20641 attctaaatt agggaatcct tgctggcgaa gtgcttcata cactatcatt gcagctgtat
    20701 tcgataaatt caatgagcgg acatgctcat cattcatagg gatgcgtaaa cacttttctt
    20761 cgttttcacg cataaatgtt tctggcaaac cggttgtttc ttttccaaat aaaaagaagt
    20821 gctcttctcc atcagcaaaa ttttcgtcac tatatacttt atgggcaaat tttgagatca
    20881 aatgtagttt acgattacct aaaaatgtta aaaaagcttc taaattactg tggtaagtaa
    20941 tatttacatc attccaataa tccaaccctg cccgttttag atgtttgtca tcagttgaaa
    21001 aacctagtgg ctcgattaaa tgaagcggag tattcgtggc tgcacacgta cgggcaatat
    21061 ttccagtgtt tgcagggatt aatggttcaa ataaaacgat atgattcatc aggatcattc
    21121 ctctcatatt tatgttcgtt tctattttct gacaatttga tttttgcata aaaaaaaacg
    21181 tatcaactcg gtgtcagtca ctgcgagtcg ttacgttagt gaagctcttc tcactaactt
    21241 ttatcagaac aggaacctga taaaaaccaa tgaaaaaaca acttgttgct aatataacac
    21301 cctcaacttt tttttgcaac ctctactcat taaaaactgc tagaaaaacg ttttctctta
    21361 gttcctttta ataattctat gtcaattgta atcttcttca aaggattctt atataagtct
    21421 gctttgattc gctcttctac ctgccattct aaaggtgaca tggccaaaag atctttttga
    21481 ttttcctctg gtatctcaaa ttcataggtt acacgctgac gttcagttaa tttcattgct
    21541 tcagcaaact tggctactac acgttcattg gaataactct gtttttcagg ttgattcgga
    21601 taaaaagcct gacgtagttc ctttaaataa ttttcttgtg gcaccacctt caacacttct
    21661 ccatcttctg agagtacacg ttggaattct tggtaatgag aaggcgaaaa gatatttaaa
    21721 accgcgttga atgtttgatc agcaaacggc aaattggtca aatcagctac acaccaaaat
    21781 gcttcggcag attgttctgt agctgcgtag actccttctt ttgaaatatc aaatccaaca
    21841 gcattttgaa tctttttcaa ttttgaaagt tcattcaaga agctaccttc tccgcagcca
    21901 acatctaata gcgagacttc ttcaggtaaa tgctgcgcaa tagtctcaat aacgggctga
    21961 tacatgccag attggatcat tctacgcctt ggaacgaaca ttccttgatc gtattctgtt
    22021 ttaaccgcat gggataaaaa ataaagtgtt ccttttttgg aaaggtcaaa acggtgatta
    22081 tttttgcata tcaagccatt atcagacttt tttacttctt cttggcaaac cggacagcgg
    22141 aacagctgat gattttctgc caaaaactgg cgtcctcggt caattttttt taacatagtt
    22201 ccccctcatt tctgttttat cttaccgtta gatgctcact ttggcaactg ctaaaaatat
    22261 gatacaatgc ctagaagaaa gtgaaagata aaggagaaga ttagatgatc aaagaatttt
    22321 gcgctgaaaa ctacacatct attcctctag caattgctag aggagccgat cggatcgaat
    22381 tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac tggtgtgatc gaagaaacgt
    22441 taagttatgc tggggaaaaa gatatccccg taatgacgat tattcgtcct agagggggcg
    22501 acttcgtcta taacgatatc gaattgaaaa tcatgcatac tgatttgatt gaagcaaaga
    22561 aattagggac ggatggcgta gtatttggtt gtcttactcc ttcaggctgg ctagacgaag
    22621 aggccttaga actattgatc gaaacagcag aagggttgca gatcaccttc catatggctt
    22681 ttgacagtat tcctgaagat cggcaattcg aagcaatcga ttggctggta gaacatggtg
    22741 ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac tattgtagaa aatttcccac
    22801 ggcttaaaga attgattgcc tatacagatg gccgcttaat cattttgcct ggtggtggtg
    22861 tcactgcaga gaatgcagca catgtagcta aagaattgaa cgtaagcgaa gtacacggaa
    22921 ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa aaacatttgc aacaggcata
    22981 atgcttattg caaatgtttt tgttttttta cataaaatag cccataaata aagaagcaat
    23041 attgaaataa atcagtacac tcgtcaaatc gctcaaagtc gtgataaagg gaccgcttgc
    23101 tactgctgga tcaaatccta gacgatccat tagcatcggg attaaactac ctgcaagatt
    23161 cgccactgtg attgcaaata gcatagccat ccctatgaca aaccccaaga taaaattatg
    23221 tttccaaaaa cctacgatta gaaaaatcgt gatccccgta acagctcctg tgacaacgcc
    23281 ggtcaagact tcagccaaaa caagtcgaac aaatccatga tctttctcat cagaaatcgc
    23341 aagtcgacga acagccacag ctagcgactg ggttccagca ttaccggcag ttcctgtaat
    23401 cagcgaaatg aagactgcta aaatactcgc ttcacttacc aagtcttcat agtgactgat
    23461 taaagaagcg gtcgacatac ccaaaaacaa cagagtgatc agccaaggca aacgtcgaga
    23521 cgctgccttt actggatttt cattaatttc ttccacattg accccggcaa gtcctgaata
    23581 atcgctagca gcttcatcat caataacgtc gatgatatca tcgactgtga cgattcctaa
    23641 caaatgatca tcataatcgg taacaggaag agctaagaag tcataatctc taaatgtttg
    23701 tgccacatct tcttggtcgt cgcctacatg aactgaaagc actcgttcgc tcatcagatc
    23761 agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc agagaaataa cccctactag
    23821 ctgaccttct tgattaacga catagatgta atagattgtt tcagctacat ctgcttcgtt
    23881 cttcagcacg tacatcgcag agcgaacagt ttgattggct acgatcgaaa caaattcagt
    23941 tgtcatgatc gcgccagccg tttcatcctc gtaatggaga agttccttga tttcactggc
    24001 atcgtctgcc gacatcaaac ttaaatattt agcaatttgc tttttatcca gcgtattaag
    24061 caagtcaaca gcattatctg tatacatctc agaaagcata tcagctgcat agctaggacg
    24121 catttcatct aggtattctt tcatatgttc gttgtcttct tcaatcacat cgaacatatc
    24181 cgctaattct ttaggcgaca gataggaata gatcagctgc ctgttctctt ctgtaagaga
    24241 ttggtagaat tgcccctgct catagatatg catctctaaa aaactgtttc tgaatgcctg
    24301 aatattattt tcccttaaat cttgagtcaa ctgggcaaat ctttcctcta attcttgatt
    24361 ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc gtctcacaac tgcctccatg
    24421 tctgcggcta aaggctgctt gaactccagc aactcttctg taaatggatg gtaaaaacgc
    24481 agatcataac aatgaagggc ttgtcttttt attcccagat ccattcgccc gccatacata
    24541 tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa ctcttatttg atgtgtcctt
    24601 cctgtatgca gctggatatc aactaacgcg atttctgtta gtcgcttctc tatccagtac
    24661 tcagtctgtg ccgaacgtcc ttcatcagag accattcgct tcaatagcga agaataatct
    24721 cgggcgatcg gcaaatcgat atgtccatgt gccgataaat ctgaaagcct tccactgact
    24781 aaggcctgat attttttcac aaacttcttt tcacgaagtt gcatatctaa ttttgcgtgc
    24841 gcaaatccat gctttgcaaa aagcatcaaa ccagatgtat cccgatctag tcgcgtcacc
    24901 acatggatta cttggttttc gtaaccttgt tttttataat aagcctttac acgattagcc
    24961 attgtcccat ttggatggta ttgggcagga atagacgaaa cgcctgctgg tttatttact
    25021 accagaacgt gttcatcttc atacacgata tcgatcggtg tttcatccaa aagtacggtt
    25081 tcatgttctc cttcagcagg aatcacaatg gttaccttat catccttagc tagagaaaac
    25141 aagacatttt ctacttgatc attaactttg atttgaccgc cttgaaattt tattttcgct
    25201 aacagaccct ttgagatccc tttttctttt aaaaaatatt ttacttgctg agaatgatct
    25261 ttctcataat cccatgtaaa ctgcatatca atcctcacta ataaaggcat ctttcacacg
    25321 atgccaaaaa tgcatatgtc gataagaagc aaaatggatc cgttcttctg ctattcgata
    25381 ataaattgag cggatatttg cttttgcgac attcaattga tcaaccgtta caagataatc
    25441 ttctgtattt tccaatttta tttctagcca ttcatcatgg gcaatcacga tcggagaacc
    25501 caaagtacga aacactcgat tattcagtga agcaatctct gccaactgaa aggcattgat
    25561 actaggatgg atcacagcac cgccgacact tttattatat gctgtcgaac cagtaggcgt
    25621 agaaatcgtc aatccatctc ctctaaaact ttcaaacagc tcgtttttga tgtatacatc
    25681 ggctaccatc gtacgattgg cccgtttaat cgttgattca ttcagtgcta aaaaatgctg
    25741 gtccggtgtt tcatccaaat aactgatccg aacatccagc aagggataac taacactttg
    25801 ttcccggttc gttcgtaagc tgtctactag ttcttccagc tcgtaatccc gccaatccgt
    25861 gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc acatcattca gacaatgact
    25921 gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg actgagatga ccaattctgg
    25981 ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt gcgttcaaac gttctgtcac
    26041 ttctatagtc tttgcttctt tattatgaac gatcgctact ttcatcccct gcctcctctc
    26101 tttcgctttc tttcttcaaa agcgttcgat agtagacatc atctgatttt cctcttccat
    26161 gggagaataa ccgctgtgct tcttggattt cttcacggat actggacatc tcttcatcca
    26221 actgataggc agcttctgcc gcacgctgaa gtcgttcact catttcttct ggaaaaacgc
    26281 cttgatattt ataattcagc gaatgctcaa tcgttgccca gaaattcatc gcaagagtcc
    26341 gaatctggat ttccgccaaa attttcttct caccgctgat cagctgtaca ggatactcga
    26401 tcaccagatg ataagaacgg tagccacttg cttttttatt tgtgatgtaa tctcgttctt
    26461 ggagaacttt catgtctttt cttttacgga tgatctcaac aacttgatgg atatcttcta
    26521 caaactgaca catgatcctt aacccggcaa tatcagacat ttcttcttct aaacgtccca
    26581 ttggaatatg ccgtaattgt gattttgtta caatactatc gactggcttg actctcccag
    26641 tgacaaattc aatcgggaca tgtcgatttt gttcacggaa ctgtttgcgg atcccccgca
    26701 gtttcacttt caattctgat acagtttgtt catatggtgc taaaaatgct tcccaatctc
    26761 gttccatagc tgatacctct acttcattaa cattcattgt ttattctacc atatatgcaa
    26821 agttttttcg tactcttgat ttatgcattg accaaaaaaa gaaaattagg caaaatagac
    26881 aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga atataaatct atgctgacaa
    26941 agcaagagta cgagatattc atcgctcact atcaattaac agataaagat ttccgtgtac
    27001 agactaatat ttatttcgat accgttgatg aacagctgaa gaaacaaaat tgtggcctac
    27061 ggatccgttt cgttggtcat caagctgaat atacattaaa aacacctgct gaaaaagggc
    27121 gtctcgaaac aacggatacc tttataacag aagaagcaga agcatttatc ttcgaaaaac
    27181 gtctgcctcg ttcaggcgca gtttttcaaa aactttctga tttgaatatc gatcctgctt
    27241 cattaatcca aaccggaaag cttacaacaa aacgggctga attccctatt gaagaaggac
    27301 ttttagctat tgatgaaagc tggggagaca atctacacga ttttgaactg gaactagagg
    27361 taggggatgc atctgtggga aaaattgctt ttataaattt tttaaaacgt ttttctttac
    27421 cttatcgtcc tgctaagaac aagatccaac gtatgcttga agctaaaaat tagaaacctt
    27481 tcattttcac aaggcacttg agttcatttt tttacttttt tctgttaagt tataggatgt
    27541 gaaagaatct agtcactagt tgtatagaat ctattttggg ggagattcat atgatcgaaa
    27601 tctatttatt tgttaatcct ttaggggaat actgtttcga aatggaacaa cagttattac
    27661 aatttatcga agaagagtac gggacaccat caaaagaaaa gatgcaattt cgctttcttc
    27721 ctttggtcaa tttgcagaca attggggatg tcatgcaaca aaaaggaatt tctcagaaca
    27781 atcttgagga acgtaatcac ttattctcaa tgacttattc tgcagcattg gactgcaaag
    27841 cggcgcagtt tcaaggaaag aaaaaaggcc gccgtttttt gatcaaattg caagaagcag
    27901 tcggctgtaa tgacatacca tattctcaag aacttgctga atccatattt gaagaaatcg
    27961 gcggcgattt gaatatgttt aaagaagatc gtcagtctga cttcgttaaa gaagtattct
    28021 tttctgatca aaaaattgct cgggaaatgg gcatcacaaa acacccttct gcagtcgttt
    28081 acaattatgc ctgcgaccgt gattttggtg ttttagtaga agtaaaaggt acaagtagcc
    28141 tttggagaat cacagagctg tgtaaaacaa cagataactc atatcaagtt tttaatggtg
    28201 aacatttaca tacaaatgat tctcagttcc attcaagaac tagccattta cacttgcttt
    28261 ctaattaaaa aaatacgtcg actcccttac aagagagccg acgtattttt ttcgtttcta
    28321 ttcgagaaaa tcaaaaatag aaagccttat aaacgatcga tcaattcttc taattcattt
    28381 agacgttgtt caaacatcga cattgcatct tcaatatatg cagcctgtgt catatcaact
    28441 cctgcttttt tcattacttc cacaggataa tcgctgttac ctgcttttag gtaagccaag
    28501 tagttctcta gtgcctctgg ttcctggttt agtattttct tcgccagtgc tgaagcagca
    28561 gaaaagccag tcgagtattg gaaaacatag taattataat aaaaatgcgg tatccgtgac
    28621 cattcgaatt taatttctgg gtcttcttcg actgctggac cataatattt tgcattcaat
    28681 ttaccataac tatcacttag gtattcacta gtcaatggca cacctttttc atcttccgta
    28741 tgcatgaaat gctcgaattc agcgaattgt gtctggcgga aaactgttcc tttaaaccca
    28801 tccaagtaat ggttgagtac ataagcccgt acacgagggt ctttttctgt ttccaataaa
    28861 tactccgtta ggatattttc attcgttgtc gaagcaattt ctgctaaaaa gatggagtag
    28921 tcgccgtaca cataaggctg gttcgaacga gtgaaataac tatgaacact atgtcccatt
    28981 tcgtggacaa gcgtaaatag ctgatccagc gtatcatgcc aattcaataa aatatatgga
    29041 tttgtgtcat agcttcccga agaataagca ccgcttcgtt tccctttatt ttcgacaaca
    29101 tcgatccaac gttcagagaa tgctttttct acgatggcca tgtattcttc acccattggt
    29161 ttcagtgctt ctaaagcttt ttcttttgct tcttcgtacg taaaggtaat tggagcttcc
    29221 cctaagaccg gtgtataaag atcatacatg tgcagttttt ccacttctaa taaacgtttc
    29281 cgtaattcca tgtatcgatg taacaaaggc aaatgcttgt ttaccacgtc taccaaagta
    29341 tcgtatacac tttcaggaat atgattattg ctcaaagacg cttctctggc agagctgtaa
    29401 ttacggactt tcgctttaaa attatgtcct tttatatgtg tgcctaaagt agaagcaaat
    29461 gtatttctaa attgttcgta aacactgtac aatcccttaa atgctgcttc gcgcaccctt
    29521 cgatccgtgc tttctagcaa ctgaccatac acgccatgag ataattggac tatttcacca
    29581 ttttctcctt caatcgttgg aaaaactaga tctgcattat tcaaaacagc aaatgtatca
    29641 cttgaagcat caaagatttc acctgctcca gcaagaagag attcctgctc agcagataag
    29701 acatgggctc gattatctac tatttgctgg atataatggc gatagacttc caattttggt
    29761 tcttctttga aatattgcca aatctggtca tctgacaatt gcaatatttc tggttcaaac
    29821 caggaaacgg cttcactaac tttcgaaaac agactgcttg ctcttgcata aagcgcttgg
    29881 taatctgtat ttccagtgtc ttgatcgttt ttaaggtgcg catatacata aatgacttca
    29941 gtttggcgat aaaccctcaa tacgaattca atcgcattta aaaattgaga agcgccttga
    30001 tcaagtgttc ctttgtgttt ttcagattgt tttaactctt ctgataattc caaatatttc
    30061 tcatcaaact cttggtcgct tgaaaagatc ttggtcaagt cccaagttaa attttcaggc
    30121 aattcttctc gttttggcaa ctgctttact tccatgaaat ccactccttt tcttccctta
    30181 gctctatcta tcaaaataga tcatagtcta acatccacat gtcacagcat ctaacttttc
    30241 tactcattca tgtgtctgct tccatcaatt ttagcataac taaatcttgt tttacataga
    30301 aaaccgattc ctagcctatt tttatactta ctattttttc aaagaagaca agtgtatgca
    30361 ttcgttaaaa aactgctgat agaccatctc ttcatcaatc ataggaaaaa gccattccct
    30421 tttatgttct tttatttggc aaagccatga gttgaaatca ggaactttct ggttttccaa
    30481 ggcttcttcg tacaacattc gataaagaaa aacctgttct tgtaaataaa agaaaaaccg
    30541 acttttttga tagatccacg atgatagatg caaaagatgt ttatgacgca agtagcattg
    30601 ttcttgtatg gatactgtat tcttttgctt gtgaataagt tggcgtgtca gccatttctt
    30661 cccatcttca ctcacttttt ctattttttt catttggttc attttaattt cgttactagt
    30721 gaatatttct ttaaggtccc tactagaaaa agtccagcag ctactgcaaa aggaaatatg
    30781 ccctgataca gtctctgtaa tatgatgata caaataaagc ttttgtttct tgagatcagc
    30841 tttccagcaa tgcactcctc tttttctatt atagtagcag caactttttt ctatcaatga
    30901 gaattgcgat tgtccaagaa aagaattacc aagtatccac catacttggt aactgttatt
    30961 caaataattt tgggttcttt caataaatct ctggtgagac agtctagaac attggatttc
    31021 tattgctaga ttcgccaaca aaatatccgg cctttgccgc aacctcggca aataggcttc
    31081 caaaaatact ggttctactg actgtcccag ccaatccatg aagcattcct ttaactgtaa
    31141 gtgctctttt gattctcttt cactaaagac cgaacagtta tctgattttt gatgggcaaa
    31201 atgagcacat tttttctttc cctttttcaa aatcactctt tttcgacagc cacgacaata
    31261 atatttcttt cctttttctg catgtttagc aaaacagcac ttattatatt cattcattgc
    31321 aagcagcatt tttctccctc tgaacttatt tacgtctaag aatttattta cacgaaaaaa
    31381 gagggcgtga aaaagctatt ctgcatcaag taataagaac agctaaacaa aaatagttcc
    31441 caatattttt cgtttggttg ggcttgttac cgcagatgca agcttttgaa cacatttata
    31501 cgaaaaaaag actgcgacac aatttttgtc acagtctctt actataatgc tgatttatac
    31561 aaaataaaat ttcgttcgct cgatagcatt acgcgccatc agacattgtc catattcatt
    31621 caatgtttct ggtgtacgat cgctcttttc agcaaactcc aaaattcgag caatctgatt
    31681 ctctacatcc gcttggttga gattttccat ccagaagtga acgactaggt aatagcgctc
    31741 ttcgaataaa tacaaatcag tccaagcaga ctctagttcg acatctgttg caagttggat
    31801 cacttgttca aaatcttcaa aagcaaaagt tgcttgtctt tcctgttctt ctagggcgct
    31861 tccagataat tcttcctccg gatcgctgcc aataatctgc tgacgaagaa agtcactcac
    31921 atcttctgaa tcaacagtag aatcttctga ttgagaaatt tcttcatccg gaagattttt
    31981 actgataaac agttctaatc cgtctccttt tggcaaaact tggaaagtga cagcttcgct
    32041 tcctcggaac tcctcttcga tatcaacttc ttccaaaata ctataaaaga aattttcgat
    32101 ttctttgtga ttgccaagta aatccaaaaa tgaaacgccc cgttcctcta aatcttcatg
    32161 accaattagt acacggatcg tattttcatt aatatgttcc atttccattt tgctacacct
    32221 cactttgcca caatgtttaa tatagattca ttgtagcgaa atcagcttaa atgtaaagaa
    32281 aaaaaatctc gctgttaagc gaatttaata aaaccagcgg atttttcaac ctttcaagct
    32341 atcttattta actataacat ttttggcaga cgaatactat atagccgact tttcagctaa
    32401 ttttccctct ctatttttcg tgcaatcatc atcttctact acctttttat acatctaatc
    32461 aagaatactt tttgtaaaaa caaaaaaact tggaaaaact atct
//
LOCUS       DAZULY010000007        28855 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000007 DAZULY010000000
VERSION     DAZULY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.007"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..656
                     /locus_tag="ACG81A_000208"
     CDS             117..656
                     /locus_tag="ACG81A_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013611.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            1676..2845
                     /locus_tag="ACG81A_000209"
     CDS             1676..2845
                     /locus_tag="ACG81A_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS5013612.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3318..3872
                     /locus_tag="ACG81A_000210"
     CDS             3318..3872
                     /locus_tag="ACG81A_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5013613.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(4045..4482)
                     /locus_tag="ACG81A_000211"
     CDS             complement(4045..4482)
                     /locus_tag="ACG81A_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5013614.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            4530..5477
                     /locus_tag="ACG81A_000212"
                     /pseudo
     CDS             4530..5477
                     /locus_tag="ACG81A_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            5748..6183
                     /locus_tag="ACG81A_000213"
                     /pseudo
     CDS             5748..6183
                     /locus_tag="ACG81A_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            6199..7032
                     /locus_tag="ACG81A_000214"
     CDS             6199..7032
                     /locus_tag="ACG81A_000214"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5013615.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            7277..8302
                     /locus_tag="ACG81A_000215"
     CDS             7277..8302
                     /locus_tag="ACG81A_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5013616.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            8440..8565
                     /locus_tag="ACG81A_000216"
     CDS             8440..8565
                     /locus_tag="ACG81A_000216"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013617.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            8590..9705
                     /locus_tag="ACG81A_000217"
                     /pseudo
     CDS             8590..9705
                     /locus_tag="ACG81A_000217"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            9788..10684
                     /locus_tag="ACG81A_000218"
                     /pseudo
     CDS             9788..10684
                     /locus_tag="ACG81A_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            10700..11524
                     /locus_tag="ACG81A_000219"
     CDS             10700..11524
                     /locus_tag="ACG81A_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5013618.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            11556..12866
                     /locus_tag="ACG81A_000220"
     CDS             11556..12866
                     /locus_tag="ACG81A_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFS5013619.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            13014..14273
                     /locus_tag="ACG81A_000221"
     CDS             13014..14273
                     /locus_tag="ACG81A_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5013620.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            14283..15155
                     /locus_tag="ACG81A_000222"
     CDS             14283..15155
                     /locus_tag="ACG81A_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5013621.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            15167..15997
                     /locus_tag="ACG81A_000223"
     CDS             15167..15997
                     /locus_tag="ACG81A_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5013622.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            16037..18220
                     /locus_tag="ACG81A_000224"
     CDS             16037..18220
                     /locus_tag="ACG81A_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS5013623.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            18281..19501
                     /locus_tag="ACG81A_000225"
     CDS             18281..19501
                     /locus_tag="ACG81A_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFS5013624.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            19498..20958
                     /gene="gtfA"
                     /locus_tag="ACG81A_000226"
     CDS             19498..20958
                     /gene="gtfA"
                     /locus_tag="ACG81A_000226"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFS5013625.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            21035..22141
                     /locus_tag="ACG81A_000227"
                     /pseudo
     CDS             21035..22141
                     /locus_tag="ACG81A_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            22463..22672
                     /locus_tag="ACG81A_000228"
     CDS             22463..22672
                     /locus_tag="ACG81A_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS5013626.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            22665..22814
                     /locus_tag="ACG81A_000229"
     CDS             22665..22814
                     /locus_tag="ACG81A_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFS5013627.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            22968..23633
                     /locus_tag="ACG81A_000230"
     CDS             22968..23633
                     /locus_tag="ACG81A_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5013628.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(23917..25002)
                     /gene="xerS"
                     /locus_tag="ACG81A_000231"
     CDS             complement(23917..25002)
                     /gene="xerS"
                     /locus_tag="ACG81A_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFS5013629.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            25336..25569
                     /locus_tag="ACG81A_000232"
     CDS             25336..25569
                     /locus_tag="ACG81A_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFS5013630.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            25566..25973
                     /locus_tag="ACG81A_000233"
     CDS             25566..25973
                     /locus_tag="ACG81A_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS5013631.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(27110..27628)
                     /locus_tag="ACG81A_000234"
     CDS             complement(27110..27628)
                     /locus_tag="ACG81A_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFS5013632.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            27995..28243
                     /locus_tag="ACG81A_000235"
     CDS             27995..28243
                     /locus_tag="ACG81A_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013633.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
     gene            28746..>28855
                     /locus_tag="ACG81A_000236"
     CDS             28746..>28855
                     /locus_tag="ACG81A_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5013634.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT         9928 a         4351 c         5287 g         9289 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgagaacctt gggttttcaa gtgctttttg
       61 gttcattaat cttaaccata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
      121 ggagccttgt aggaatagtt cttattattt ttactgtgac tgcagcaaca gaagcaaatt
      181 attcgcataa aattcagtcg tataaaggtc gtttagttat gaccaaaaaa gatgttacac
      241 gttatacatc ctcaagaact ggaggcctat atgactatgc accattagat gcattgaaat
      301 ataaagatca tttaaagaaa catgaagtaa aagttggtga tcggtgggag aatgcatctc
      361 ataaagcata taattccaaa atagtctata cagtagaaga tgccgatgag caaaatatca
      421 cattacctgt attcaattat tggggaacag ttgtgaaagt aaatgggaaa aagagcacta
      481 tagaaaatga caatggttta gtgtctatta aaggtacggg gaaaagaact atagttgagg
      541 tttcatctac gtatccaact acgatgattc tatctttatt aattagttca ataactttta
      601 tattcttatt tacacgttat ttaataagat gctgcggtga aatgaaaata gattagatag
      661 atattataaa tatgttgatt ctattttcat agcaatggag aaaatacatt agcaatatat
      721 aaaagaaggt atcaagagtc acagtgaatt tattccgaaa agtgaaaaga gcaaatataa
      781 aatttaatga aaatatagaa ctaaatagga aaacgtagac gtaattttct acgttttcct
      841 tttcatatat attaaatatt acggaaaatt atataaacta atttagtgtt ttgtttgtaa
      901 ttcctaattt tgctccactt ttagataatt tttaggtcta tttaactcat cagacattct
      961 atgctcattt ttctccaaat ttgtttcaac tctttcgtac ttaattttgc gtatttgaaa
     1021 attcttcttt tagagtactt ttattacgat actagtatga tatatatcta tagcaaacag
     1081 ttaaaatctt cctaaaataa gcgaatagca ttcctaaaac ctataaaatt tttagatgaa
     1141 gcgacaagtg gacaattaat aaactacttg aacatgaaat tatggatcac ctttcaaatt
     1201 tagaaaatgg aattcttata cttattgcgc aacatttatt gattgcaaaa ttatgtgaca
     1261 aaaaaaacta gtagaagaaa gaataatggc ttgataataa agtactacct attaaaggta
     1321 gtactttatt taatttagta taaattaaca tactactata ttttaggttt catattatat
     1381 aaattaataa tatactacca tttgttggta gtataattta aaggtaataa gttattcttt
     1441 tattactatg tttgggtttt atagcaaaga aagtttaata agagtttgga cctagcaaac
     1501 aatattaatg aggtcattaa atcttattat ctttagtgat ataacgaaat atctacacag
     1561 acaaaatgat agcgtaaaat attttgtgta aatagatatt tagggtagaa aaaaagaaag
     1621 tccattctgt aaaattaaag ttaccacaac cgaaatttta taggaggact ttctcatgcc
     1681 caattttact acagaaatta tggaaacact aatcaataaa ggtgatttag atgaattatt
     1741 tcgatgtcat ttagaattag ctgttaattc attattgcag gctgaattaa cagcttttct
     1801 tgattatgaa aaatatgatc gtgctgggtt caattcaggt aattcccgta atgggaatta
     1861 ttcacgttca ttcagaacag agtatggaga attaaatttg gtaattccta gagatagaaa
     1921 tggtaagttt tctcaacaaa ctttacctgc ttataaaaga accaatgact ctttagaaac
     1981 cactattatt cagctatttc aaaaaggaat tactatgtct gaaatctctg aattgattga
     2041 aaaaatgtac gggcatcact atacgccaca aaccatttca aatatgacta aaatagtttc
     2101 tgaagatatt attgccttta aagaaaggtc cttagaatcc agatactctg tcatttttat
     2161 ggatgctact cacattcctt taaaaagaca aactgtttca aaagaagcag tttatattgt
     2221 aattggcatt cgattagatg gaaccaaaga ggttcttggg tttagtcttg ctccaactga
     2281 atctgcttat gtttggaaag agattcttca agacctaaaa gaccgtggtt taaaagaggt
     2341 tttattagtc gtaactgatg gtttaagtgg cattaacgat agtattcata gtgtttatcc
     2401 aaatgctcaa tttcaacaat gttgtgtcca tatatctaga aatatcgctc ataaggttcg
     2461 tgttagtgat cgacaagaaa tctgtagcga tttcaaatta gtttatcaag caagttcaaa
     2521 agaagaagca aataatcaaa taaggtttat gatagataaa tggaaaaagc agtatccacg
     2581 agtggttaaa ttgctcatga atcctgctat attaaccttt tataatttcc ctccgtcaat
     2641 cagaagaact atttattcaa ctaatttgat tgaaggtttt aacaaacaat taaagaaata
     2701 taccaaaaga aaagagcaat ttcctaatga agaatctctg gagagatttc tagtatctca
     2761 attcaacaac tataatcaaa agtttctatg ccgagttcat aaaggtttta aagaaataca
     2821 tgatacatta gaatcaatgt tttaacttga tcaacagaat gggcttttcc atttacacaa
     2881 aattattgac gctcccgaca aaattatgat cttgcgtaaa gttataaaat attaataaaa
     2941 atattttatt aatgaaaagc ttggtagcac ggttattcat atgctaattt atttcacttt
     3001 atagtaaggg gagttcttag tatattgttc aaacttaaat ttcatataat ttaattatgt
     3061 aaattagcta ttctaagaac ttctacggat aactgcccgt ctaaaattta aataagagct
     3121 tagaatcttc atataagtaa ggttaatttt tttgtacaaa aaagtcggaa tattctgagt
     3181 tactgaaatg atataaaatc ataaactaaa tcccttgatt ttattgactt tcaaaagttt
     3241 atatatataa gttaatgatt atggtaaaat atatacataa tcattaactc agaatatttt
     3301 taagaaggga aacaatagtg agctataatg tacaaccgtt aaggacaaat gaagagatac
     3361 aagattttca gttttggcta agaagaacaa gtaatccgga aagagatagt tttttatttt
     3421 tgtttggaat caataatggg ttacgtatgt cagatattat aggattaaaa gttggagata
     3481 tacgaggcaa aagtaagcct attattgttg aacataaaac aggtaagcga aaaccgattt
     3541 ttatagataa tttgagagaa gaaattttat tatatactga aggtaaagaa gaaaatgatt
     3601 ggttatttcc gagtcggcaa caaggtcgac atataacaag ggatcgtgtc tatcagatat
     3661 atgcagatat agcagaaaaa ttaggacgag atgatatagg aacacataca ttaagaaaaa
     3721 cttttgggta tcactactac aagaaaacta gagacattgc taccttaatg tttattttta
     3781 atcatagctc gcaagcaatt acaaaaaggt acattggtat aactgaagat gaaataggag
     3841 catcacttcg tggatttaaa ctaggtgtat aatttaaaaa tttttataaa cagtaaagac
     3901 aaagattaaa agctattttt atctagaacg attttatttc ataaagttcg cggaataaaa
     3961 gctggggtta ctggtacttg gtataggttc gtgttatata aactaagtaa ttaaataaca
     4021 tttttaggtt ggaaaaggat gaaattaaaa aaagagaatc aatacggtct caaaaacgca
     4081 aacgctgcgg aataagaaaa atactgataa ctcaacgttt agcagactat ctaataggga
     4141 tagtctgctt ttttcgtttt tgaccatact tttgaccttc tgcaaacttc cttgcttttc
     4201 ctctgtagaa ctcatgaaat ctgcaaaacg aattgccgtt tcctctgtct gctttttcgc
     4261 gatatgggtg tatatatcat ccatagtggt tttaatatct gtatgtccaa gtcgaacata
     4321 cacttctttg acagacgcac cagattcaaa gagcagacta caatgggtat gacgaaaacc
     4381 atgaagtgtg atatgtttta agttattctc tttaatgatg attttcaaat tgtgatttac
     4441 atagtctaaa taaaggggct tattttcttc tgatgaaaac aaaagttgtt caccatcttt
     4501 tataggtttg attctcattt ttagatatta tgcccgcacg tattcatcac atttctattg
     4561 tgaatcgctt tattcgaccg acatttgact tttatcataa tatactcggt ttaaaattac
     4621 taatgaaaac aattaatcaa gacgatcata ccatgtatca cctgtttttt tcagataatc
     4681 accaccgtgt gggaactgaa ctgacttttt ttgaagtaca agaagggaac aaccagtttt
     4741 ttggaaccaa tacgattgaa cgaacgatac taaaagttcc ttccgaagca tcattgcatt
     4801 tttgggaaat atattttgaa acacaaggcg tttgccatta tggaatcgaa tcattttctg
     4861 gacgacctat cttacgtttt gaagggccag atgcaacacg attagcttta gttcccttac
     4921 gcgaatttga agatattgat gattatttcc cgtatgtcac agaccagatc cctacagagc
     4981 atgccatttt aggaattgat gccattcaac tacgcgtaca atattccgat gcgactgaac
     5041 gagagttatt gtcggtaggc tggtagcaca aagaatacgt cagtttcttt tcgggtcatc
     5101 aacgagtgac aatcttagaa aatcaagaca ctcagttcta tcaagaagtt cagattattc
     5161 aagatcgtga gaatccagtt gccattgaag ggattggtag cgtccatcat gttgcgtttg
     5221 gcgtagaaaa taaatctgat ttacaacgca tcgacaaaca attacaagag cgcaatttta
     5281 taaattcagg aataaaagat cgtgaatttt ttgtatcact ttattatcgt gacccaaatc
     5341 aattattgat tgaaattgct acaggggaag gaaacctaga tgcaaaagcg tatgaaaatc
     5401 aaagtccacg ctttgaagaa attcctttat ttttaccgca gtacttaaat ttcattcgtg
     5461 aacaagtgga gcaataatta gctaatcaaa tccatgacga agagtaagag gttgactcat
     5521 aagtcaaaaa ataaagaaaa acgcatcaaa tccattctaa agaatgttga tttgatgcgg
     5581 ttcctatttt tataaactat cattttcgac taataaaact acttatgagt cagcctctct
     5641 ttttttcttg atttttatac ctctttctag gtaaactaaa catctgccaa aaataccgtg
     5701 tctcattgaa acattctcaa ctatttcaaa ataaagggga gtaccaaatg aacgagtata
     5761 ttgaaattat ccacgcaaga gaaacaactt aaaaatgtca gtctcaaaat tcccaaaaat
     5821 aaaattagta tttttaccag cgtatctggt tcaggaaaat catcgattgt ttttgatact
     5881 attacccaag aagctggtcg tcaactgaat gacacctatg atagttttac ccgtttattt
     5941 ttacctaaat acagtcgtcc agaggttgat gagattgata atctttctac ggcgattgtg
     6001 gtcaatcaaa agaagattgg tggcagtgcg cgctcaacgt tagggacgat tactaacatc
     6061 aatgcgttat ttcgtgtgtt gttttcatgt tttgcaacac ctagtttagg atatgcaaat
     6121 gcttattcat ttaatgatcc tagtggaatg tgttctgtct gtcagggaat cggtaagacg
     6181 attgcatttt taataggatt gctttatttt tcggctattg ataatgctga ttttgtattt
     6241 tctgatccat ttaccgcaga agttttgtct ggaattgagg aacaggcaaa gaatcatggt
     6301 tattttgtat tggtgcataa tgttaattca gcagaagatg ttaaaactat tcagagaaat
     6361 tggcgttttg aggggttcat tgttgttggt gtacgtgtaa aagaattttt ggaattaaat
     6421 aacgtttttg atggtcctgt tgtttatatt gatacgcatc tttcaaaaga gacattaaga
     6481 gaacttgaac aacaaatgaa tcgtttattt gtaaatacaa atgattacca agctagtaca
     6541 ttggcagtgg aacatttgat tcattatggt catgagaaaa tagcgtttct ttcttttgac
     6601 tacattgtag gtgaaccaag tgttattcaa gaacgacatc aagcttatat cgatacatta
     6661 aagaaatatc atttaacctt taaatcgcat tggatatata acaactctga atttgagcga
     6721 atttatcaag aattagctga atttactgca attgttgtta ctggagatta tttagcggct
     6781 aaattagtaa aatttttaaa aaataagaat aaacgagtga tggaacaatt atcgattatt
     6841 ggatttgatg atattacttt tgcagaatta atggatcctg cgttgacaac aatacgacta
     6901 aatccaggta acaagggggc gattgctttt caccaactga ttggactggc caaacaagaa
     6961 gaaataaaaa atcaagtact actattagag ggagaattga tttctcgaga ctctgttcac
     7021 aaaatcaaat aaaaaagctc tgtgaaataa gtatagttaa caataaaata tccgaacgat
     7081 agagagaatg aactgtgatc tagcagttcc tcgtcgcaaa ttcactagaa ttagcattat
     7141 ttactctaaa ttgttcggat attttttctt gtttttaatt atgattacag tgcctatttt
     7201 taacgaaatt tgcttatttt caaaatgtat gattatatgt tagtaaacac tttcaaatat
     7261 gaaaagagga gctattatgg taggaattag ggatgttgca aaacgggcgg gtgtgtccat
     7321 tgcaacagta tcgcgcgtgc taaatgagga tccgacactt tcagtaaccg atgaaaccaa
     7381 acgaaagatt gcagaatcgg ttaattttta tcattatgtc aaaaaaagtc ccgttgcaaa
     7441 aaatttaaca aatattgcat taataacgac agtttctgaa gtagatgagt tggaagatcc
     7501 ctattttcga acgattcgca gagggattca agtcgaagca gaacaaagca aagtcaattt
     7561 gaagaaaatt attcgtttgt cagaaacacc gttggagcaa gacgatttgc taaaatgtca
     7621 aggtgtgttg attattgggc aggtgctatc ttctgtgatt gaagaagtat cagcaattaa
     7681 ttcgaatgtc gtagtgattg atgaccctag tattgaagct gaagtagatg cagtttatac
     7741 tgatttaaca aaagcaacga atcagcattt agatcgattg tataaaaaag gacaccgaaa
     7801 cattgcgttt atcggaggag gacgtgttcg tttagatagc caaggaaata aacacgtgtc
     7861 agatgatgat tatcgaaaaa tagcctatga gacgtggatg aaacaaaaag gattagaaaa
     7921 atatagtcaa atttatttag aaggctggac cacacttgac ggaatgcagg cagcagattt
     7981 acttttgaaa aaacatgcta aagataagtt gccgactgct atcatggtag gaaatgatcc
     8041 tattgctgtg ggggtttatc gttcacttca aaaaaatggt ctacatattc cagaagatat
     8101 ttcgattgtg agttttgata atattgaagt tgctgaattt ttaacacctg cattaaatac
     8161 ggtgaatgtg aatacagaag aaattggaaa actagcagtt cgcatggtta atgaacgaat
     8221 aaaagaagtt agaaatgttt cgatcaaagt gattgtttct aataatatta ttgttagaga
     8281 aagtgaaaaa aatatcgatt aaaaaagtaa acatccaaaa aagggtgttt acttttttgt
     8341 ttactaatat ttttagtaaa aaatctcgac aaaacattaa gtaaacagtt atggtgttgt
     8401 taacaaaaag aaaacgcatt caaaacagga ggaattaaga tgaaacattt caaaaaaata
     8461 gctttaacag ggatagctgt tttaactggg acattactcg cagcatgtgg tgggggagat
     8521 agtaattcta aagacgggca agtaacttta agctatgcga gttgagatac tgaccaagct
     8581 ccaggactaa gaaaaatgtt agatgaattt gaaaaacaaa atcctgatat caaagtagaa
     8641 atggagacaa cgccttggga tcaatattgg gttaaattag aagctgcaac aacgggaggt
     8701 aatatgccag atgtagtaac catgcactct agcgaatcct ataaatatat gtcacagaat
     8761 gctttgatgg gacttgatga tgttatcaaa gaaaatgata ttgacatggg aaactttact
     8821 gaaggaattg ctgatttcta tacgcttgaa gacgaactat acgcaatccc taaagatgct
     8881 tctgtagtgg ggctatggta caacaaagaa ttatttgata atgcaggtgt cgattatcca
     8941 gatgaaacat ggacatggga aacactcaga caagctgcaa ttgatttaac agatgaatca
     9001 aaaggcatct atggttttgc atcacatatt ggaacagaga ctggctactg gccatttatt
     9061 tatcaaaatg ggggagaagt atttgccgat gacaatacaa aatcaggttt gaatacccca
     9121 gaagcacaag aagctttagc tttttatcga gatattgcac ttgttgatca tgcttcacca
     9181 acaattacag aattacaaga tcctgataag ttaagtcgtt ttcaatcagg tagattagca
     9241 atgatgattg aaggaaactg gtttgcaccg ggatttttga aaaatgacta tctagttgaa
     9301 aatgttgctt ctgccgtgtt acctaaggga aaagttaatg caacaattac aaatggactt
     9361 ggttgggctg ccagtgcgag tacaaaatac ccagaagaag tgaaaaaact aatggcattt
     9421 ttagctagtg aggacgccaa taaaatccaa gcggaaacgg gggcatcaat cccagcacta
     9481 aaaggctttg aagacgagtg ggctgcttct tcagatacaa ttgatttgag tgcttttgga
     9541 aaaatgttgg agtatggagt ccctcgtcca tacaataaag atggattacg agctgaacaa
     9601 gcagaagttc aaattattaa ccgtattctg tctggtgaag taactgttga agatggaacc
     9661 gctcaaattg aaaaagaagt caatgcgata ttgagcggaa attaaattag ttaattatac
     9721 aatatacagg cgaaaggatt ctatttcgtc tgtatattaa tggatgaata ccaaaaaggg
     9781 ggatattatg aaaaatcgaa tttttggaaa atatcgtaaa gatcaagtga tatggggcta
     9841 tatttttatt tttccaacgg cattaggatt aattttatta aatatatggc cagcaattca
     9901 aacagcaatt ttaagttttc aaaaaacggt agggttcggt gatactgaat gggtcggatt
     9961 aagaaattac caaaaaatgt ttgcagacgg tgaagttttt cagtctttat taaacacatt
    10021 aatttatgca gtggtttcga tacctagtat tgttgtatta tccttgttgg tggcagtttt
    10081 aatgaataga aaagttaaag gattgtcgat ttatcggaca atttattttc taccagtcgt
    10141 tgctgcacca tccgcagtcg caatgatttg gcgctggatg ttcaataatg attatggact
    10201 gattaataac atgttggcta aaataggtct cgatggacca gcatggttaa cggatccatc
    10261 gattgcgatt tattctatta tcattgtagg agtttggagt gctattggtt ataacatggt
    10321 gttattacta gccggcttat aagaaattcc taaagattat tatgaagcag cggaaattga
    10381 tggtgctagt ccaatccagc agtttttcaa cattacgtta ccattagtta caccaacatt
    10441 attttttgta gttgtaacaa cagttatcaa tgctttccaa gtatttgatg ttatttttat
    10501 gatgattact cctaatagta ccgccatgtt taaatcacaa tcattagcct atttattcta
    10561 taaacattca tttattttaa atgacaaggg atatggttcg gcaatcgtta tgttcctttt
    10621 agtgattata ttaattatta ctgcaattca aatgaagttg cagaaaaagt gggtaaacta
    10681 ttaaggaggc tatcaaatta tgaaaaataa atttttttca aatgcgccaa tccatatcat
    10741 gcttatttta ggtggtattg caatggcatt accttttatt tggatgattt taacttcagg
    10801 gaaaacgttg gttgaatcta cccaaattcc acctacaatt ttccctgaga aatttcagct
    10861 aagtaactat caacaagtac ttgatttatt accattcatg gctttttata tcaatacatt
    10921 tttaatggtt gttggacgag tgattggctc ggttttattt tcagcaatgg ctgcatatgc
    10981 ttgtgcgcgt ctaagttttc cagggaaaaa tatccttttc agcttagtac ttattcaaat
    11041 gatgattccc ggttccattt ttattactcc acagtattta ttagcacaaa aattgggtat
    11101 tttaaataca attccagcat taatattacc aggggtagtt agtgcgtttg gtacgttttt
    11161 attaagacaa tttttcttag gtttacctga tgaattggag gaagcggcct atttagaagg
    11221 tgccaatatt tggacgatct ttttcaaaat tatgttgccg cttgctaaat caggattggt
    11281 tgcacttgga atattcacag cgttgtttgc atttaaagat ttaatgtggc cattgattgt
    11341 caatacttca attgataaga tgcctttagc tccgggatta gcaaacttac aaggacaata
    11401 tgccacgaac ttcccacaat taatggcagg ttcgttatta gcaatttggc caatagttgt
    11461 gatattcatc gttttccaaa gaaaattcat tgaaggaatt gctacaacag gtagtaaatt
    11521 ataaaactat taattagtga ttggagagta agtacatgac aaaaattaca tttattggtg
    11581 caggaagtac tatttttgca aaaaacgtat taggcgatgc gatgttaact ccaagtctac
    11641 aagatgcaca tattgcattg tacgatattg atgaaaaacg actaaaagaa tcaaatttaa
    11701 tgcttaaaaa tattaataaa aattcgaatg aaaatcgtgc tgatattgtt tcttattatg
    11761 atcgcaaaga agcgttaaga gaggctgatt ttgttatcaa tgctattcaa gtgggtggtt
    11821 ataaaccttc aacagtgatt gattttgata ttcctaaaaa atatgggttg cgacaaacta
    11881 ttggtgacac catggggatt ggtgggattt ttagaaccct acggacacta ccagtaatgt
    11941 tagactttgc taaagaaatg gaagaagttt gtccaaatgc ttggttattg aactatacaa
    12001 atccgatggc gatgttaacc atgggtgttt taaaagcgac gagtattaag actgttggtt
    12061 tatgtcatag cgttcaagta tgcgtaccag aattgtttga gcatttagga atagccgatc
    12121 aatatgactt aaatgacttt caatggaaaa ttgcaggcat taatcatatg gcattcttgt
    12181 tggaaattac aaaaaatggg gaagatttct atccaacaat tcgtcagtta gctgcagaaa
    12241 aaccaaatcc acatccagat tctgtacgtt ttgaattgtt gaaacgtttt ggttactata
    12301 ttacagaatc aagtgaacac aatgcagaat accatccata ctttattaaa tcaaaatacc
    12361 cagaattgat tgacgaatta gggattccgt tggatgaata cttacgtcgt tgtgaacaac
    12421 aaattaatga ttgggaaagc atgcgagagg acattgtaaa taatggggag ttaacacata
    12481 cgagaagccg tgaatatgct tcatatatca tggattctat tacgacagga actccgtcag
    12541 tgattgcagg aaatgtctta aataaaggct taatcacaaa cttaccgaca gatgtttgtg
    12601 tggaagtacc ttgtttagtc gataaaaatg gcgtgcaacc aacctatgtc ggtgaattgc
    12661 caacacaatt agctgcttta aatcggacga atatcaatgt tcaagaactg acagtagaag
    12721 ccgctttaac actagataaa tcaaaaattt atcaagcagc gtatttagat ccacatctat
    12781 catctgaatt gtctttaaca gatatcaaaa atttagtgga tgatttaatc gaagcacatg
    12841 gggactattt acctgaatat aattaaaaag aagctgaaag agtaaggaat tttcaattcc
    12901 ttactctttt cttagttaaa tttagtaaaa atattgacaa aacacttagt aaacagttac
    12961 attagtgtta acaaaaagaa aacgcattca aattgtataa ggaggagcat aaaatgaagt
    13021 ggtctaaaaa aattatttta gcttgtatag cactaggagc aacgatctct ttgactgctt
    13081 gtggaaaaaa tcaggaagat gcaagcggta aggtagaaat agagtttttt aatcaaaaaa
    13141 aagaaatgac tcaaacgatt caggagattg ctaaagattt tgaagcaaaa aaccctgata
    13201 ttcatgtaaa agttgtggat gtaccgaatg ctggtgaagt aattaaaaca cgtatgcttg
    13261 caggcgatgt accagatgtc attaatcttt atcctcaaag tatagaattg aaagaatggg
    13321 cgaaagctgg ttatttagag gacttaaccg aagaaccgta tttagagaat atcaaaaatg
    13381 gatatgcaca acgttttgct attgaggatc gtgtatatag cataccatta accgctaatg
    13441 tctatggttt ttactataac aaaactgctt ttgaggaaat ggggattcaa gcaccagaaa
    13501 cttgggcaga atttgaaaaa atagtagctg agattaaaga tcaaaacaaa gtaccgtttg
    13561 cgattgcagg agcagaaggc tggactttaa atggttatca ccaactagct atagcgactg
    13621 ctgctggtgg tgaagaggaa gcgaacaatg tttggcgttt ctcagaagtt aatggaatta
    13681 atgctgaaag caaagaaatg caaaaagatt ttaatcgatt agatttgtta agagaaccaa
    13741 aagctttgca aaacaactgg caaggtgccg gttataacga tacagtagtt acttttacaa
    13801 aaggtgatgc gttgattatg ccgaatggtt catgggcaat gccgatgatt cattcacaaa
    13861 atccagattt tgaagtagga acttttcctt ttccagcaga tgaagcaggt caaagtctaa
    13921 cgattggcgc aggagaccta gccttatcag tttctgcaac ttctcaacat aaagaagaag
    13981 ccaaacgttt tgtagaatat atgacaacac ctgaagcgat gcagaaatat tatgatgtag
    14041 atggtgcacc gtgtgctgtt gagggcgtta tcgagaatac tcaggattca ccattaagtg
    14101 gtttaactga acttgctttt actgatcgtc atttagtttg gttagccaaa gattggaata
    14161 gtgaaaatga tttttatacc ttaacaacaa attatttaca caatggcaat caaaaagcca
    14221 tgattaacgc attaaatgct ttctttaatc caatgaaagc tgacggaagg taggaaaata
    14281 aaatgaaaat aaaagaaatt ttaaatcgtt actgggcaat catttttgta gcaattccaa
    14341 ttattttgca gatgattttt ttctactttc caatggttca aggtgctttt tacagtttaa
    14401 ctaattggac tggtctgact tataattttg attttgtggg tttgaacaat tataagattc
    14461 ttttttctga tccgaagttt atcaaatcga ttggcttcac tttaattgtg acgctttgca
    14521 tgattgctgg acaaattggt tttggtattt tgattgcgcg ggcgttgaac gcaaatatta
    14581 aagggaaaac attctttaga gcatggttct tttttccagc ggtcttatcg ggcgtggttg
    14641 tttctttagt atttaaacaa atttttaact atggtttacc aaaaattggt agtatgttag
    14701 gcattgaatt tttacaaacc agtcttttag gaacaacatg gggagcgatt tttgcagtaa
    14761 tttttgtttt actttggcaa ggaattgcca tgccagtcat cattttctta gcgggttttc
    14821 aaagtattcc acaagaaatt attgaagctg ctagaatgga tggggcgaat gataagcaaa
    14881 ttttttggaa tattgaaatc acctacttat taccaagtat ttcaatggta tttattatgg
    14941 cattaaaagg tggcttaaca gcatttgatc aaatttttgc tttaactggt ggtggaccaa
    15001 gcgatgccac aacatctttg gggttacttg tttataatta tgcttttaaa aacaatcagt
    15061 ttggttatgc gaatgctatt gctttagtct tatttatctt aattgggatt gtttcactgg
    15121 cacaattaaa attatcgaaa aaatttgaaa tctaggaggt ttcccaatga aaacatcaaa
    15181 agaaaaaaat atttctaaat acttgctttt attattaggg ggcttcatga ttttagttcc
    15241 cttagtttca acgctattcg gttcatttaa aacaacaccc gatattatga ataatttctt
    15301 tcgttttcct aatccagcga ctttggaaaa tttccgaaga ttgttagcgg atggaattgg
    15361 tcattacttt tggaattcgg caatcatcac aatcattgca gtagttgctg tgacagcaat
    15421 tattcctatg gctgcttatt cactagcaag aaaaatgact cgtgggaaag catacggatt
    15481 gatgtattta tttttaattt taggaatttt tgttcctttc caagtaatta tgattccgat
    15541 taccgttatg atgagtaaaa ttggtttagc aaatatgtgg ggattaatct tactctatct
    15601 cacttatgcc gtaccacaga cactattttt atatgttggt tttattaaac taaatgtgcc
    15661 tatcagtctg gatgaggctg ctgaaattga tggggctagt cactttacaa cttatttcaa
    15721 aattgttttc ccaatgttaa aaccaatgca tgcgacgact attattctta atgctctatg
    15781 gttttggaat gatttcatgc taccattatt aattttaaat cgtgattcta atatgtggac
    15841 actaccattg tttcaatata attatacggg acaatatttt aacgactatg gtccaagttt
    15901 tgcttcctat acagtaggaa ttgtcacgat tacactagtt tatcttgctt tccaaagaag
    15961 tattatagct ggaatgagca atggagcagt gaaataacca ataaattatt ctcattataa
    16021 aggagttcat aaacttatga ttatatttga caatcacaca caaacatttc atttacaaaa
    16081 taaaaaaatt agctatctat taggaatcga agaaaataat tatttaacgc atctttattt
    16141 tggaaaaaag attacgcact attctggtgg gtatcgctac ccccgaacag atcgttcctt
    16201 ctcacccaac aatgcaaatg cgcaagatcg tttattatct ttagacacat taccattaga
    16261 atttccagga tatggacatg gtgattttag agaaccggcg cataatatta aattaggaaa
    16321 cggtagtcgt gtaaatgatt ttcgttatga tagctacgaa attcaaaaag gaaagcccag
    16381 cttagcggga ttgccagcct tatatacgga agatgatgat gaagctgaaa cgctgattat
    16441 tcaattgaaa gacgcgatta gtggattgac tttacagttg ttctacacaa tttatcaaaa
    16501 tcacagtgta attattcgtt ctacgcaatt agtaaataat agtgatgaaa cagtggagat
    16561 taaccgctta gctagtcaat caattgattt tccaaatcgt ccactcgagt taattcattt
    16621 aaatggtacc tgggcaagag aacgtcaaat gactagagaa aaaattcata cagggataaa
    16681 agtattagat agtaaacgcg gatcaagcag ccatcaacaa aatccgtttg ttgctttagt
    16741 agatgccaaa acagatgaat ttcaaggaga ggcatatgga tttaatttag tttatagcgg
    16801 taatcacgaa acggtgattc aaaaagatcc atttgaccaa acacgggtca ttatgggaat
    16861 taattcattt aattttggtt ggcaactaaa aactggcgac tcatttcaaa cgccagaagt
    16921 cgtgatggtt tatagtaatg aaggtctgaa tacgttatca cagacgtatc atgaattgta
    16981 taatcaccgt ttaattcgtg gaaactatcg tttaaaagaa cgtcccacgt taatcaacaa
    17041 ctgggaagca acgtattttg attttaacga agaaaaaatc atgaatattg tcgatgatgc
    17101 gaagaacttg ggcattgaaa tgtttgtttt agatgatggt tggtttggcg gtcgtgaaaa
    17161 tgacttcact tcattaggtg attgggttga aacagaaggc aaacttgctt gtggtttgga
    17221 aaatttagcc aagcaagtgc atgataacgg gatgaaattt gggatttggt ttgaaccgga
    17281 aatgatttct gaagatagtg aactatttcg agcacatcca gattgggcat tgggtattcc
    17341 aggtcgtgac cgctcaatta gtcgaaatca atatgttctt gatttttcaa gacaagatgt
    17401 tcgtgataat atctatcaac aaatgacggc gatttttgat cgtgtaccga ttgattatgt
    17461 gaagtgggat atgaatcgaa atatgacaga agtctattct gtcttgttgg actcaacgat
    17521 gcaaggtgaa gtggctcatc gttatatgtt agggttatat gaatttttag aaaagttaac
    17581 aacgaattat ccaactattt tatttgaaag ttgttcgggt ggaggcggtc gttttgatgc
    17641 aggtttcttg tattacatgc cacaaacttg gacgagcgac aataccgatg ccgtggctcg
    17701 tctgaaaatt caatatggaa ccagcttagt gtatccaatt tcaagtatgg gtgcgcatgt
    17761 ttcagcagtc ccaaatcatc aaacaggaag agaaacgagt ttaactattc gtggcaatgc
    17821 tgcgatggct ggtgtttttg gttatgagct agatctaacg caattaactt ctgaagaaaa
    17881 agcagaaatt aaagaacaag ttgcctttta taaaaagcat cgccaacttt tgcaatatgg
    17941 aactttttat cgcttggaaa gtccgtttga aggcaatgat actgcatgga tgtttgtctc
    18001 aaaagaccaa acagaagcca tggtctttta tttccgagta ttagcagaag ctgcgcatcc
    18061 attagtcaca ttgaaattag caggattagc agaagaagca acttatcaaa tggaagatgt
    18121 aacatttgct ggcgatgaat tgatgaatct cggtttttat attgactcag aactacatgg
    18181 tgattatgcg acacaacgct tttatttgaa acagatatag tagcttgaat ttacaatata
    18241 agtgcaacac caaaagagac tcattccaaa cgaagaaaat atgttttatt tacacgcatt
    18301 tactaaaaag cagcctgatt tgaattggga aaatgaagaa gtacgtgaag aaatctatgc
    18361 aatgattaac tattggttag ataaaggact aggcggattt agaatcgatg cgatattaaa
    18421 catcaaaaaa aaactagagt atggcatctt tgaatctgat ggagaagatg ggttagcctt
    18481 cattgggccc tggattctta atcagtcggg cattgaagtg tggttaaaag aaatgagaga
    18541 gcggacattt aaaccacata atagtatgac ggtagcagaa gccgatgttc ctaatgaacg
    18601 tttagatgaa tatattggtg aagatggcta ttattcgatg gtttttgatt ttagttatac
    18661 ggatattgat gtacctgaaa ctggtgaatg gtttaaagat agccaatgga catggacgga
    18721 tatgcgtacc aatattttta cgaatcaatt agttacgcaa gataaaggtt ggggcgcctt
    18781 atatttagaa aaccacgatc aaccacgttc aattaataaa tatattccag aagaatggat
    18841 caatgattat agtaaaaaaa tgttggcgac gctttttatg ttactaagag gtaccccttt
    18901 tatttatcaa ggacaagaac taggaatgac caacatagaa atggcatcat tagaagattt
    18961 tgatgatgta gctacgcatt ctcagtataa acgggcatta gaatatggat tggcaccaga
    19021 gcaagcattg aaagctgtca ataaacgaag tcgggataat tctcgcacac ctatgcaatg
    19081 gaatacgcaa aaaaatgcca gattctcaac ttctgataat gtatggttaa aagtaaatcc
    19141 taattatcca gaactcaatg cagaagctca gttaaccaat caacagtcgg tattcaatta
    19201 ttatcatcaa ttaattaaat taagaaaaaa tagtgtatac aaagacgtgc taatctacgg
    19261 acaatttcta ccagtgaaag ataaaaatga acatattttg ctttatgaac gccaattaga
    19321 agataaaatc gtattagtta tcttaaacgt tacagcaaca gaacaagaat attatgtaga
    19381 tgaacagtac caacaagtat taatcaataa ttatgaagat gttttattag atcaaagtcg
    19441 aaaattgcgt ttacgaccgt ttgaaagtat tgtattagct aattatggag gacaattatg
    19501 aaaattaaaa acgaagcaat gctcatcacc tatgcagata gtttaggaaa aaacatgggc
    19561 gaattaaaac aagtcttaga caaacattta caaggtgtta ttggtggtgt acatctgttg
    19621 cctttttttc catcgacagg tgatcgaggc tttgcgccga gtgattatac gacagttgat
    19681 ccagctcttg gaacatggga agatgtggaa gcattaggtg aagattatta tttgatgttt
    19741 gattttatga ttaatcacat ttctcgtgaa tcaaaatttt tccaagattt caaaaaaaac
    19801 catgagcagt caccatacaa agaaatgttt atccgtattc atgaattttt cccagaaaat
    19861 cgaccaacac aagaagatat tgatttaatc tacaagcgta aagacaaagc gccgtttcaa
    19921 acggtagaat ttgcggatgg tacgacagaa gaggtttgga atacctttgg ggaagaacaa
    19981 attgatttag atgtgaccaa agaagttgtg aaagaattta ttcgagaaac catcaaagat
    20041 atggcgaacc atggctgttc tttaattcga ttagatgcgt ttgcttatgc gattaaaaaa
    20101 ttagatacca atgatttctt cgtggaacca gatatttggg aacttttaga cgaagtgcgt
    20161 caagaagcag cgaaatatga cgtcgaatta ttaccagaaa tccatgagca ttattccatt
    20221 caaatgaaaa ttgccaatca tgattattat atctatgact tcgccttgcc aatggtcact
    20281 ctgtattcac tttatagtgg aaaatcagaa cgtttagcca attggttgaa aatgagtccg
    20341 atgaagcagt ttacaacatt ggatacgcat gatggaattg gtgttgtgga cgctcgtgat
    20401 ttattaacag atgaagaatt ggattacacc tctgaggaat tgtataaagt cggggcgaat
    20461 gtgaagaagg tctattcaag tgccaattac aataatttag atatttatca aattaacagc
    20521 acctattata gtgcattagg cgataatgac cgtagctatc tgttagcacg tgcgattcaa
    20581 gtatttgcgc cagggattcc acaaatttat tacgttgggt tattagcagg aaaaaatgat
    20641 attgaactgt tggaagcaac gaaagaaggg cgtaatatta atcgtcatta ctatgattta
    20701 gccgaaattg aagaagaagt gcaacggcca gtgattcaac aattatttgc acttttaaaa
    20761 tttagaaatg aatctgcggc gtttgatttg gatggaacga ttgaagtagc aacgccatca
    20821 gaatcagaaa tagtgattac acgtaaaaat aaaaacggta gtgatagtgc aacattaacg
    20881 gctaacttgg atacgaaaga atttatcatt gagtctaaag atataaaaat tttcactagt
    20941 gttgcattaa aattttaacc tattccatag aacaaaaaat cctttatact agcacttgga
    21001 tgacttatcc aagaccaata aaaaggactc atacatggat aagtataaaa caaatacagc
    21061 atttaataaa tggttttctg ccataaaatt aggaaaactt tctgtcaact cccaaaaatg
    21121 tatttttgat tacaattcgt atcgtaaaaa actgtctttt gaagctgtgc tcaagctctt
    21181 tctctacgca gttaatgaag aaaaagaaag tttgcgtgat ttgagtacag ctcttatcga
    21241 ccgatcgctc caactagaaa ccgatgtgtc atcgattagt catacccaat tgtctcgagc
    21301 atttaatgca cttgaaccta aagtgttaga agaaattttt caacaactgt tagaaaaagt
    21361 tcaacatcaa atcaaaccaa ccaagcgaaa tcatctgtat ctgattgatt ccagtacgtt
    21421 ttcgcttagt ttaaaacgtc ataagtgggc gactttccgt caaacaaaat caggtgtcaa
    21481 gttacactta aactattgtt atatggacga tactacgatg tatccaactg attttacctt
    21541 aacaaatgcc catgaacatg atctcaatca actaccagta ttggtgaatc aaccagaggc
    21601 aacttacgtg tttgatcgtg ggtattagga ctttgaaaag atggatcaga tgcattggga
    21661 tggctatttc ttcgtcactc ggattaaaaa aaacaccaaa gttcacgtga tagatactct
    21721 ggaaacatcg ccagaaacag aaattctacg agacgagctg gttcgtttag gatctaaaac
    21781 ctatctaacc gccaatttcc gactagtcac tgttcaagat aagaacggac gtgtgtttca
    21841 atttatcacc aatcgaatgg acgtctcttc aaaagaaatc tctgatatgt atcacgctcg
    21901 ttggcaaatc gaattgtttt tcaagcacat caaacaacac atgaccatta aaaccttctt
    21961 ttctcaaagt gaaaaaggcg tgcagaatca attgatttta acgatgattt ccgctctcct
    22021 cacgtttctc atcaaattgg agacgaaaac ggaaaaatca gtgttccaaa tcaaacgatt
    22081 ctttcgttat ttactgtttc agccctttga atgttgtcta gaaaaattaa ttcccacatg
    22141 agaaaaaaga aatttggctc gttacttttg gttttcagtt gaataacaga taaaaaattt
    22201 tctggaaaaa gtcatcctct gcgtcagcac agtttttttt gacttatttt cagaggaaat
    22261 agttttcaga atattctaaa atgttttatg caacactagt gatatttttt agaattagat
    22321 ttctaatgtg cattttttga gtattaagga gcaagtatac aaagatttat aaagctgatt
    22381 aaatttgaat aaaaatatgc aggaacgcta ctatttttag tagtgttctt ttttatattt
    22441 gaataaaaaa tgtggtggtt aaatggtggt tataacaaaa aatgaattaa tgaaattagg
    22501 ttatgggaag tatcagtctg agagaatcat acgacaggca aaagcaatta tggtttcaaa
    22561 aagctatacc tattacgaaa atagaagatt aggactagta cctgttgaat ctgtagaaga
    22621 agttttaggt atgaagatag aggtagagga ggattttcag aatcatggtt aattttaaaa
    22681 aagataaaaa tgggaaatat cattttactg ctagtttggg attcgacaaa cgaacaggga
    22741 aaagagtaca aaaaaacgta gaggggttaa aacagttaaa gaagaaaaaa agcgtatacc
    22801 gaacttgttt ctaattatgg aaaagaagca gaaacttctc aaagtactat gttgtttgag
    22861 gagttttttt ataaattatt cctaccttat tatcaaggga aagtagaaga aagaacatat
    22921 aaaaatcggt tatctacaat caaaaaaact tttcttattt ctttaaaatg gagttaagag
    22981 aaatacgtcc aattgacatt caaaagtggc aaaataattt attgagcaaa tataaaaatt
    23041 cttatgtaag aaacatgtat gggatttttc agatgtcatt agatcgagca gttgttttgg
    23101 gactacttga aaaaaatcct gcaaaagtta ttggaaatgt gaagaaaagg aaaactacag
    23161 ttgatttttg gacaaaagaa gaatttacaa aagttatttc aactttttat attgatgatt
    23221 tgtaccaaca ttttggtttt atttgtatgt ggttactttt tatgaccgtt atgcgtttgg
    23281 gagaagccac tgcattaact tggaaagata ttgattttga aaataaaaac ttgcgtgtga
    23341 ataaaacact ctattataag agagccaaaa cctatgagtt tgtagaaccc gaaacggctt
    23401 ctagtattcg tacaatcgaa ctagacgaca atacaattac ctttttaaaa gcttggaagg
    23461 agcgtcaagt ttcaatagga ggcatgtcgt ttgtactttc ttataatgga acacctactc
    23521 agaaatatac aatccgtcat attatgagaa gacatgcaac gatcgttggt gttcatataa
    23581 tccgcataca cggattaagg catagtcatg cttctttgct tatatcacaa taaaaagatg
    23641 cacttcgaat taaagaaagg ttaaaacatg ctgatgtaca aacgacttta ggaacttatg
    23701 gtcatttgta tctaaattca ttgagagaga taacaaatga tcccacagat tatatccaaa
    23761 ctaatttttc taaaatcaaa ccgacaccta tttacgataa taaaaaataa atgataaaca
    23821 aaagtaggat cacaccaaaa gaaacacact agaaaaacta taatacaggc ttttctagcg
    23881 tgttgtttct tatttccgcc tatttataca ttatactcat aaagcattta atgcatcgat
    23941 cgtagccgca ttgtctatat gggtatataa agttgttgta ttggtacttg aatgtcccaa
    24001 ttgttgggca gttaatacca atgaatttgt ctgttggtag agtctagtag ctaatgtatg
    24061 acgcagtttg tgagggctga cttgaattct aaagcctttt gaatattttg ctaccatccg
    24121 ttcgattgca ataccggata tacgcttggc ttctccttgg taaattgcta gaaataaagc
    24181 tttttcgttt tctgttactt tatatcgttc tttccgaata gctagataat tagctaaata
    24241 ttccattgcg aatgctgcta tattgacagc atctctttta tttccttttc tagttactac
    24301 cacattattc ttatctaaag ataggtcatc taaattaata tttataacct cggagagtct
    24361 caatccagat cctaaaatca gtgcattgat cgccaggtct ctttctacat ctcgttgata
    24421 atagatcaaa gctcgtttac tcaaagtatt tttatattca ttggcaatat aatctagata
    24481 attttgtgta tccttatcta gaaataattt tttctcaatg gcattggcac gtgcagctaa
    24541 cgtagtttta tctgtgatgg ttgctacttg atccatagga ttattgtcta agtatggttt
    24601 tcctagagga ttattggaac ttttcgaaag atatttaaat agaaccttta atgccgtgat
    24661 atttctattg atagtagaag tagatagacc taagttatta ttatctttga gcttagtttt
    24721 tgttgtctct ttttttacat tttcttttgt gcgacgtttc aaatgagatt tgtacaagtt
    24781 taggtcatag gcagttaact gtcttatcga ggtgatagaa gtatcaggaa cgatagactc
    24841 actcagaagc cattcataaa agagcttata attttttaga tattctaaag tggttcgagg
    24901 tgattgatct ttatcctcac gatcttgaac aaaatctttt atataatcgg gcatatgaag
    24961 tatgattgta tctatttttt gatatatttc tgtatttttc atgtcattca tcctttcaat
    25021 aagtagggga gaagagtatc tctataattg gactatacca attatagaat gttttgtcaa
    25081 ttggtatgct ctaaaattta ttaatttgtt tcaaaaaaag catataaaca ggtatacgtt
    25141 taataattta aaataaatgg tatacataaa tagggaagga aatagaaggg agcaatctaa
    25201 attttttgaa ttttcttaat taattatata gaaagaacaa tctttctata gagttagaca
    25261 tgagagggac tatgcttgat ttaaaatcaa caagcgtata tactgtatat acaaagaaaa
    25321 aggagggaga agactatggc aattaaagaa agaaaattta gggaagtggg gaactcagta
    25381 gtattaacta tttcaaagga gtttcttaaa gaaaatgggt taaatcccgg agatacggtt
    25441 tttttagatg aagaaaaact tcgagcggca attacaaaaa aacaagcctt atctgatgaa
    25501 attgatttag caatatctca agcattaact gaatatgata ctacttttag agagcttgtc
    25561 gataaatgag tattcattac ctagaagcca aagatattat aaaaatgaat gtaatccaaa
    25621 taaaaaaata ctctcctaat gaaccccttg gtgtaaagga tgcgaatgct ttagaaatgt
    25681 gtgtagggca acttcgagcc tcagcttttg gtgaagaggt ttatccttcg atttatgaaa
    25741 aagcagctat cctacttata caattgataa agaaacatcc ttttcacaat gcaaataaac
    25801 gtactgcttt cttagctact tttgtctttt tgaaaatcaa tggacagctt ttagctattg
    25861 atcaaaaaga tgctgtaaat ttagcggtat atattgctac gtatgataag gatttcgatc
    25921 aactaaaata tgaagtgatc aacactataa agacacatac gcagcccatt taaggagtta
    25981 ctgtatgtgt ctttataggg ttattagctg ctatagcgaa ataattgaag taaattacaa
    26041 atagctataa ttcaaatcca actatataga aaacgtaaat tttctatata gttggattaa
    26101 gataacgact atttatttta tcatattttt actataccct atacttttac ttggtatatt
    26161 tatgaataat cgcaaattta cccctatact actagtttgt aagttagatt aataaaaata
    26221 ctaaagttat ttgttctttt attaaataca cctacaactt ataaattgat ggtatttaga
    26281 taacacgaaa gaataaaaat aacagctgat agtaaagttt gttgacgatt gctactcaat
    26341 cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact tatgaaatga aagacctttt
    26401 acatcttgca gaaatcggta ctgggattaa actaagcaat aaaaactaat gttcccctca
    26461 ttctttgaac ttctactatt tgatttattt attctgataa atgacgattt caccttcagg
    26521 atcttcaaat cccgtataga atttatttaa gatctcttca gtatcagtaa taggataggc
    26581 gataggccac ataccctttg atggttagaa taaactgctc aaaatctgta tcgtatgttg
    26641 gctttagcat attaacaaag gctatctcca aattataaac cataagcgtt gaatcgttct
    26701 taacaatttc aattcttcct aacccaaagt cagaagatat aattaccgga cttgccttac
    26761 ccttcatgtg ggttgttgag attccatatt taaagggaat tgtcggtact atcggcggtc
    26821 tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta gtctcctaag tccctttgta
    26881 gcggatatgg caccataatc tcattttatc gaagtgagtc atggtttatc tgttggccaa
    26941 ccaaaattga tacaatctaa cgatgcaccc tctacggcat tctaggggag tgcacttgca
    27001 ttcacccaat aaaaaccggc agttagcaaa gaacttatgt agtcgtatgt tcttactgtt
    27061 aacatacaag aggtctcctt tccacaattg cttagcagta aacgggaatt tacaaagcta
    27121 atctcttcca ttttcttatc tttgttctaa aagtcccaaa tggagcaact gtatttacat
    27181 gaataaattt atatacttcc catacagccg ttttggttgc ctcgtctgcc cattttcttt
    27241 tgtgtgcagt gaatagatct tcatcactca ttgaatcaat catcatgtta atatcatcaa
    27301 tattacgatt gagaatttcc tctaattctt tcaatggtag atgtgcatag gtatttgtaa
    27361 accacttata taactctcct aactgattcc atttaaattg atcagaaggc gttttaacag
    27421 ataatccatt tctttcatca ttttcccatt ttagaattaa attcgtccaa ccaacttgat
    27481 atgctaaatt ttcagcaggt gttctatcca cttcctcaac tcttttatcc ttcaattctt
    27541 caggaatagt ttcaaattct aaaatatatt tagtataagt cctatttatt tcttttttta
    27601 attcttcttt atttttatat gttctcatta tattacctcc ataaattcct aattgtctcc
    27661 tttaattcat ctataatttc atccacgata ttttaatccg ttctacaatg catatctgat
    27721 atgcattgac tgtctaactc atatgtgaat gatttataaa atatcccaac gtctcatcta
    27781 aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa tatatttctt ttgtagtaaa
    27841 atcggactgg tttgcatatc attttcttcg taaatatcaa aatcatgtta actactatca
    27901 attactaata aaaactacca ctttaagcgt taagaactgt taagtttagt taagtgagga
    27961 tagttcacaa aatccattgt taaacttcac gaaagtggta aaactcaaaa tgctttatat
    28021 aggattacag catcgctctt tcttcgattg cgcgttgggt aaaaaatatt atgaagtaaa
    28081 aattgacgat gacacgttcc ttacggctca tcagatgaaa cagctacaaa aacgaaatga
    28141 tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca tattcacgcc tcgctcgacg
    28201 aactattaaa ggtcaagatt tacctgaaga tcatcctttt taggaaattg aagcttttta
    28261 tgttgcacaa tgcatttata atactacaat gatgttttca ccagatattc ttctagggtg
    28321 cggtgtaatg aaacagatac acttgcaaaa gaaaattcac cgcgaattta ctaaaattat
    28381 gaataattat gttaatcttc cgaaattttt ttccaaaatt aagggatcaa gctggtgtta
    28441 tagattgttt aaggttggca aaaaaataac ttctttttaa acaattatag aaaaccgata
    28501 ctctgttaag ttggttgaca cggtgtcgat ttttatgact ttcttattct gaaacttcac
    28561 taacggacag tacctaaaaa gtctatagta tggacgttta cgagtactat tttatcaaaa
    28621 atgctgctca tttcaactta tatgttttag tgaactaccc ccacttagct tggataggct
    28681 acaattaact agacagaaaa attaaggtgt gtagactaga agaaaacata ccaggaggaa
    28741 tttttatgtc taagagaaca cgaagaactt tttcacaaga attcaagcaa caaatcgtca
    28801 atctttactt agctggaaag ccacgtgtag aaatcattcg agaatatgaa ctaac
//
LOCUS       DAZULY010000008        27937 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000008 DAZULY010000000
VERSION     DAZULY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.008"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(112..471)
                     /gene="rplT"
                     /locus_tag="ACG81A_000237"
     CDS             complement(112..471)
                     /gene="rplT"
                     /locus_tag="ACG81A_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFS5013635.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(524..724)
                     /gene="rpmI"
                     /locus_tag="ACG81A_000238"
     CDS             complement(524..724)
                     /gene="rpmI"
                     /locus_tag="ACG81A_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFS5013636.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(796..1320)
                     /gene="infC"
                     /locus_tag="ACG81A_000239"
     CDS             complement(796..1320)
                     /gene="infC"
                     /locus_tag="ACG81A_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFS5013637.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1364..1489)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1640..2272
                     /locus_tag="ACG81A_000240"
     CDS             1640..2272
                     /locus_tag="ACG81A_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS5013638.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2274..3179)
                     /locus_tag="ACG81A_000241"
     CDS             complement(2274..3179)
                     /locus_tag="ACG81A_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS5013639.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3264..3923)
                     /locus_tag="ACG81A_000242"
     CDS             complement(3264..3923)
                     /locus_tag="ACG81A_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS5013640.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4239..5183
                     /gene="mvk"
                     /locus_tag="ACG81A_000243"
     CDS             4239..5183
                     /gene="mvk"
                     /locus_tag="ACG81A_000243"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFS5013641.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5176..6153
                     /gene="mvaD"
                     /locus_tag="ACG81A_000244"
     CDS             5176..6153
                     /gene="mvaD"
                     /locus_tag="ACG81A_000244"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFS5013642.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6166..7251
                     /locus_tag="ACG81A_000245"
     CDS             6166..7251
                     /locus_tag="ACG81A_000245"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFS5013643.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7248..8312
                     /gene="fni"
                     /locus_tag="ACG81A_000246"
     CDS             7248..8312
                     /gene="fni"
                     /locus_tag="ACG81A_000246"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFS5013644.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8561..9364)
                     /locus_tag="ACG81A_000247"
     CDS             complement(8561..9364)
                     /locus_tag="ACG81A_000247"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFS5013645.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9384..10226)
                     /gene="kduI"
                     /locus_tag="ACG81A_000248"
     CDS             complement(9384..10226)
                     /gene="kduI"
                     /locus_tag="ACG81A_000248"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFS5013646.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10242..11249)
                     /locus_tag="ACG81A_000249"
     CDS             complement(10242..11249)
                     /locus_tag="ACG81A_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFS5013647.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11256..11900)
                     /locus_tag="ACG81A_000250"
     CDS             complement(11256..11900)
                     /locus_tag="ACG81A_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS5013648.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12000..12758)
                     /locus_tag="ACG81A_000251"
     CDS             complement(12000..12758)
                     /locus_tag="ACG81A_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFS5013649.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12885..14036)
                     /gene="fucO"
                     /locus_tag="ACG81A_000252"
     CDS             complement(12885..14036)
                     /gene="fucO"
                     /locus_tag="ACG81A_000252"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFS5013650.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14118..14429)
                     /gene="rhaM"
                     /locus_tag="ACG81A_000253"
     CDS             complement(14118..14429)
                     /gene="rhaM"
                     /locus_tag="ACG81A_000253"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFS5013651.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14431..15276)
                     /gene="rhaD"
                     /locus_tag="ACG81A_000254"
     CDS             complement(14431..15276)
                     /gene="rhaD"
                     /locus_tag="ACG81A_000254"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFS5013652.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15290..16546)
                     /gene="rhaA"
                     /locus_tag="ACG81A_000255"
     CDS             complement(15290..16546)
                     /gene="rhaA"
                     /locus_tag="ACG81A_000255"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFS5013653.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16543..18003)
                     /gene="rhaB"
                     /locus_tag="ACG81A_000256"
     CDS             complement(16543..18003)
                     /gene="rhaB"
                     /locus_tag="ACG81A_000256"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFS5013654.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18156..19025
                     /locus_tag="ACG81A_000257"
     CDS             18156..19025
                     /locus_tag="ACG81A_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS5013655.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19151..20050)
                     /locus_tag="ACG81A_000258"
     CDS             complement(19151..20050)
                     /locus_tag="ACG81A_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5013656.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20215..21039)
                     /locus_tag="ACG81A_000259"
     CDS             complement(20215..21039)
                     /locus_tag="ACG81A_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS5013657.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21050..22345)
                     /locus_tag="ACG81A_000260"
     CDS             complement(21050..22345)
                     /locus_tag="ACG81A_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFS5013658.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22361..22840)
                     /locus_tag="ACG81A_000261"
     CDS             complement(22361..22840)
                     /locus_tag="ACG81A_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFS5013659.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22846..23874)
                     /locus_tag="ACG81A_000262"
     CDS             complement(22846..23874)
                     /locus_tag="ACG81A_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFS5013660.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23891..24997)
                     /locus_tag="ACG81A_000263"
     CDS             complement(23891..24997)
                     /locus_tag="ACG81A_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5013661.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25226..27823)
                     /gene="adhE"
                     /locus_tag="ACG81A_000264"
                     /gene_synonym="adhC"
     CDS             complement(25226..27823)
                     /gene="adhE"
                     /locus_tag="ACG81A_000264"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFS5013662.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         7876 a         6131 c         5040 g         8890 t
ORIGIN      
        1 ttttagactt atacgttatt gtcctgtttc atttgatgga ggattgtaaa taaaaaaaga
       61 ggaaagaaca taacttttcg ttatcttctt tcctctttat aattgtttag cttatttagc
      121 caaagcttct tttgcttggt cagccaatgc agtaaatgct gctgcatcgt taacagccaa
      181 gtcagctaac attttacggt tgatgtcgat ttcagcaact ttcaaaccgt gcattaattt
      241 tgagtagctc aagccattca tacgagctgc tgcgttgata cgagcaatcc acaatttacg
      301 gaagtcgcgt ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat tcattacttg
      361 ttcttttgct gttttaaata aagtgtgttt tgaaccgtag taaccttttg ctaattttag
      421 cactctttta cgacgtttac gagttactgt tccaccttta acacgtgcca tgtataattc
      481 ctcctaataa aaataaatct tcagattctg ttatttgtac ttcttatttc atttttgcta
      541 gttgttggcg aatacgtttg aagtcgccac tagaaaccat gcttgcttta cgcaattgac
      601 ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg aagcgtttca
      661 atccgccttt accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt ttttgttttg
      721 gcatgactat atttcctcct caaaatcttt cgttatccga ctatcggctg tccaacagcc
      781 agttagtcat tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga acatgctgcg
      841 tccgtccatt ttcgcttttt gttccactgt tgcaatatcc gcagtttctt cagctaagcg
      901 attcaagact ttttgaccaa tttctttatg ggtaatggca cggcctttga aacggataga
      961 agctttcact ttatcgccct tttccaagaa cttacgtgca ttacgaagtt ttgtattgaa
     1021 gtcattcaag tcgatcgtag gacttaagcg aacttctttt acattgatca ctttttgttt
     1081 tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt agtccatgat
     1141 tcgcgcaact ggtggtttcg caccaggggc aactaacaca agatccaagt ttgcttgttc
     1201 tgcaatattc aatgcttctg cttttgtctt aactcctaat tgctcaccat ctgaaccgat
     1261 caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc atatcctttg ctatggtcat
     1321 tcacctccaa atattatgag agaaagaaaa gcttaccgaa taaacggcaa taaaaaaacg
     1381 agttctctta cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa ctatctagcc
     1441 cagtgacatt atcaactaag gcgagaagcg ggtgcttctg cttttatttc tcaaccatca
     1501 tagtatagca aaaactaatc tttttgtcaa tgtttttcat acagtttgtc ttccttttct
     1561 gaaaatgatt agattagcag actttctagc agtgattccc gcttctttgc tatactaaag
     1621 aaaaacgagg tgaacccaca tgaaaaaaat cgaacctatt ccaccttctc ttcctcttct
     1681 tacaaaggga gagttttatt tagaagatga ggagatcttc tctggtcttc tgatcaacgg
     1741 gggtgaccaa agctatctcg atgccaaaaa tatggtatta agagagagtc atatccaaaa
     1801 acttacgatg caaaaaacag ttttacgtca ttttgaatgc agtaatgtca tttttgaaaa
     1861 atgtgatttt tccaatctgg agtggattgg tggcagtttc caccaagtcg tgtttcatca
     1921 atgtaaatta acaggtacga attttgccga aagttatctt cgagactgta ctttcactga
     1981 ttgtattgct tcgatggctt cctttagtag tacaaacttg aaagctgtct ccttcgatca
     2041 ttgtcaatta gaagacagcg aattttatga ggtcacttgg aaaaaccttt ttttgtctga
     2101 caatcagttg accggttcga actggtttcg aacttctctg aagggattag actttacgac
     2161 taatttcttc acagcaatcg ctttatctca agattctttg gcgggactaa tcgtcaacca
     2221 agagcaagca cttgtgatcg cccaagccct tggtttgcag atcaaagact agatcaacga
     2281 atccgccgcc acagtgcaat aagcatccaa atagataaga ccaaactgat gatcaaggcg
     2341 attccccatc cgaaaacgga atgggtaaag ggtaactgga gattcattcc gaagaatcct
     2401 gtcacaatag ttggaacggt caagatcagc gaccagaccg tcaaaaattt catcgtgtca
     2461 ttcaagttgt tattcagcaa attattataa gtgccagaga gctggtctaa aatctgcgaa
     2521 gctaagttcg tcatttcaac tgcttgtcgt gcttcgatca gcgcatcgtc caactcttct
     2581 ttttcctttt ctgacaactt gcgataaata gccaatgcct tgatttgttc taaaagtact
     2641 acattttgtt ttgttcctgt aactaagtaa accaaaccga tctctaaatc tgaaagttgc
     2701 agcagattct tgttcgttgt tttttctctt aatttcagat taagccgctg acgttcacta
     2761 ttcacttcct cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt acgaaacaat
     2821 aaactgtata cactcatgtc tggcattcgt tccaaaagcg atcgaattag tcgaatcacg
     2881 tactcggtat tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat aaaagcgata
     2941 ggactagttt cataatgatt atcgactttt tgctggtgcg gaacatttga tacaagcagt
     3001 aacgcttcat caaaagaatc gtactctacg cgcgcccgtt catttttatc tagagaataa
     3061 gtcagcatct cgtcactaat gccatattct tcttttaagt ttttaacagc tagcacatcg
     3121 ttttgctgta cattgatcca aaaattacgg tcttgattaa aatagtgttt ttcccgcata
     3181 ctctatcctc ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa taaaggttta
     3241 ccctttattt gagcaactgt aaatcaaatg gctactttgg tttggcgaat ctctgctaat
     3301 ttttcttcga tcattttcaa gacttcttct tttgcttgag gatcttccac aaaattatag
     3361 cggtctccat cgatttggat ttttggactt tctttgtatt cttcatacca ttgatcgtaa
     3421 cgtctgttca attctttgta ataatcgtat agacttggat caaaggacaa ttgttcatac
     3481 gaacgaccac gtttttcgat acgctccaac atcgtatcaa aagatacacg aatatgaact
     3541 aataaatccg gatgtttttt atgggcagca tagggaagtt cttccatcat gttttccagc
     3601 agttcatcat acacacgaac ttctgtttct gttgctcttc ctagatctgc atttagatgg
     3661 aaaagcagtg aatcttcata aatcgaacgg tccaatacat tattgtcatc tttcattgct
     3721 tgtttgatgc ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa agcatactgt
     3781 tcagggtttg cataaaataa gggcaagact tcattatcat caatcgattc ataaaatgcc
     3841 tgactatcaa agtgttcagc catcatttct gttaaacttg attttccggc accaattgta
     3901 cctgctaaaa cgatcacgct catttacctc cagctcactt catcactaaa tattgtgttt
     3961 aactccaacc tttttaaata tacccccaat atctagtagt gtcaattgta tttttttcta
     4021 gtcaaagaag aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc tttagaaaac
     4081 gtattcttag ttttacttct gagatggttt tagtaaaata aaggaggaat gttttttgag
     4141 acccttagtg gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg ctttattttt
     4201 tgcttaaaaa tcacttttac gtgagaagga gttagattat ggcaaactat ggccaaggag
     4261 aatcaagcgg aaagatcata ttgatgggcg agcacgcggt tgtttatgga gaaccagcga
     4321 ttgcctttcc tttctatgca acaaaagtca ccgcattcct tgaagagctg gatgcaatgg
     4381 acgatcaact ggtttcttcc tactattcag gaaatttagc cgaagctcct catgcattaa
     4441 aaaatatcaa aaaattattc attcacttaa aaaaacagca tgacatccaa aaaaacttgc
     4501 aactgaccat tgaaagcacg attcctgctg aacgtggaat gggatcaagc gctgcagtcg
     4561 ccacagcagt cactcgtgct ttttatgatt acttagcatt tcctttgtct cgtgaaatac
     4621 tattagaaaa tgtccagctt tcggaaaaaa tcgcccacgg taatcctagt ggaatcgatg
     4681 cagccgctac tagcagcttg cagccgattt attttacaaa agggcatcct ttcgactact
     4741 tttctttgaa catcgatgct tttttgattg tcgctgatac aggaatcaaa ggacaaacaa
     4801 gagaagccgt caaagatgtc gctcacctct ttgaaaccca gcctcatgaa actggacaaa
     4861 tgatccaaaa attaggatac ttgacgaagc aagcaaaaca agcaatcatt gaaaattcac
     4921 cagaaacgtt ggcacagaca atggatgaat cacaatcact tctggaaaag ctgacaatca
     4981 gcaatgattt tcttaatcta ttgatccaaa cagcaaaaga taccggggca ttgggtgcca
     5041 aattaactgg cggtggacgc ggtggctgta tgattgcatt agcacaaaca aaaacaaaag
     5101 cccaagaaat cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg atacaaggat
     5161 taggagtaca tacctatgtt taaaggcaaa gcacgcgcat ataccaacat tgctctaatc
     5221 aaatattggg gtaagaaaaa tgaagaactc atccttccaa tgaacaacag cctttcgtta
     5281 acattggatg cattttatac agagacagaa gtcatctttt ctgatagcta tatggtagat
     5341 gaattttatc tagatggcac cttgcaagac gaaaaagcaa caaaaaaagt cagtcagttt
     5401 cttgaccttt ttcgtaaaga agccggcctc tcactaaaag cttcagtaat cagtcagaat
     5461 ttcgttccta ccgcagctgg attagcctcc tctgccagcg ggctagctgc tttagcaggc
     5521 gcttgcaata ctgctcttaa gcttggatta gatgatctct ctctttcaag atttgctcga
     5581 cgcgggtctg gttcagcttg ccgaagtatt ttcggtggtt tcgtcgaatg ggaaaaaggc
     5641 catgacgact taagttctta cgctaagcca gtcccttccg attctttcga agacgattta
     5701 gcaatggttt tcgttttgat caacgaccag aaaaaagaag tgtccagcag aaatgggatg
     5761 cgtcggacag tcgaaacatc cagtttttat caaggctggt tagattccgt tgaaggggat
     5821 ctatatcaat tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg agaaacgatg
     5881 gaaagaaacg gactaaaaat gcatggaacg actttagctg cccagcctcc attcacttac
     5941 tggtctcctg acagcttaaa agcgatggat gcggtgcgac aattgagaaa gcaaggcatt
     6001 ccatgctact ttacgatgga tgctggccct aatgtcaaag ttttagtgga aaacagccat
     6061 ttatctgagg tacaagaaac atttaccaaa ttatttagca aagaacaagt aattactgca
     6121 catgctggtc caggaattgc gattattgaa taaagggaga tttttatgat tgaagtatct
     6181 gcaccaggca aactctatat tgccggagaa tatgcagttg ttgaaacagg ccatccagca
     6241 gttatcgctg cagtcgatca attcgtgaca gtcactgtag aatctgcacg aaaagtcgga
     6301 agtatccaat ctgcacaata tagtgggatg cctgtacgtt ggacaagacg caacggagaa
     6361 ttagttttag atattcggga aaatcctttt cattatatcc ttgctgctat tcgcttgact
     6421 gaaaagtatg cacaagaaaa aaacattctt ttatcctttt atgatctgaa agtaacaagt
     6481 gagttagaca gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc cgtgaccgtt
     6541 gcaacggtca aagccttgaa tgttttttac gcgttgaatt tatctcagtt ggagattttc
     6601 aagattgcag cactagccaa tttagcagtt caagataatg gttcctgcgg cgacatcgct
     6661 gctagctgtt atggtggctg gatcgctttc tcaaccttcg accatccttg gctccaagaa
     6721 caagaaactc agcattctat cagtgagtta cttgccctgg attggccagg tctatccatt
     6781 gagccattga ttgctcctga agatttacgt ttattgattg gttggacggg tagccctgcc
     6841 tctacttctg atttggtcga tcaagttcac cgttcgagag aagataaaat ggtggcttat
     6901 cagcttttct taaaaaatag tacagaatgt gtcaacgaaa tgatcaaagg gtttaaagaa
     6961 aataatgtaa cgttgatcca acagatgatt cgaaaaaacc gacaattact gcatgattta
     7021 tctgcaatca ctggggtcgt catcgaaacg cctgctttga acaaattgtg taatttagct
     7081 gaacagtatg aaggagccgc aaaatcttct ggtgcaggtg ggggcgattg cggaatcgta
     7141 attgttgacc agaaatctgg cattcttcct ttaatgagtg catgggaaaa agcagaaatc
     7201 actccactgc cgttacatgt ctatagcgat caaagaaagg aaaaccgatg aatcgaaaag
     7261 acgaacatgt ttcactagct aaagcctttc atgataaaca aaaaaacgaa tttgacttcg
     7321 tacggatcat ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat ctaagtacac
     7381 aggcagttgg gtttacacta agcagcccat tttatatcaa tgcgatgact ggcggaagcg
     7441 aaaaaacaaa aaaaatcaac caagatcttg caatcgtcgc aagagaagca gatttgatga
     7501 ttgccactgg ttcagtcagc gctgccttga aagtcccttc tttagccgat acttatacga
     7561 ttatgcgaca agaatatcct cacgggaaaa tcattgccaa catcggtgca gggacttctg
     7621 tcgaaagagc caaagaagct atccgattgt ttcacgcaga tgccttacag attcatttga
     7681 atgcacctca agagttggtt atgcctgagg gagaccgtga ttttaccaat tggaaagtac
     7741 ttattcaaga aactcaaaca gccattgacg tccccctcat cgtaaaagaa gtcggttttg
     7801 gcatgacaag agaaacgctc aatgatctag ctgctttagg agttcataca gttgatatca
     7861 gcggtcgaag cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc aagcgggaac
     7921 tgagttattt agctgactgg gggcaatcga cagtctcttc attgcttgaa gcaaatgaag
     7981 cagacacttc tatggaaatc ctagcctccg gaggtatccg caatgcctat gacatattta
     8041 aagccctttg tctcggcgca aatgccgtgg gtacttcagg aaccgtactg acgcatctga
     8101 tgaatcacgg cgtagaagaa actattatac tgatgaagca atggcaagaa gagcttcgtc
     8161 tgctttatac tatggtcggc gcaacgaata ccgcggccct acaccaacaa tctctgatct
     8221 tttccggacc agtgaaggat tggtgcgaag caagagggat cgacttagtg aagtatggaa
     8281 gacgcacaga aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca
     8341 cgtacaaacg gcggaagtaa aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
     8401 tgaaaagcaa ttttcgtgaa ttccttctta tttcttggag ttaaatgctt ctgtcccagc
     8461 ctcttttttc gaataaatga tgtttaatca gctgtccttc ggtattcgtt caatccgctg
     8521 atttcacaac ctctccacta aacagtgtac taaagctctt ttaaggttgt tttccgatat
     8581 aggcaagtat gcctccgtcc acatacaaga catggccgtt aacgaaatca gatgcgctag
     8641 aagccaagaa gacagatggt ccagccaaat cgattggctc gccccagcgt gctgctggcg
     8701 tacgtccaac gataaattga tcgaatggat gacgttcacc gttttcttgt gtttcgcgta
     8761 atggagcagt ttgcggtgta gcaatataac ctggtccaat tccattacat tggatattgt
     8821 attgaccgta ttcagaagcg atgtttttcg tcagcatttt taatccgcct tttgccgcag
     8881 cataagcact gaccgtttcg cgtcctaatt cactcatcat ggagcagata ttgatgattt
     8941 taccgccgcc tttttcgatc atatccggaa taactgcttt tgaaacgatg aatggggcat
     9001 ttaaatccac gtcgattact tggcggaagt ctgccgcatc catttcgatc atcggtgtcc
     9061 gtttgatgat ccctgcattg ttgactagaa tatcaactga acctacatct tctttgatct
     9121 gttgaaccat cgcttgaacc gcttcttcat cggtcacatc gcagacataa ccttttgcat
     9181 cgatgcctgc ttctttataa tgcgctatac cttcatcaac tgattcttga ttcaagttat
     9241 taaatacgat ttttgctcct gctgtagcta aagagcgtgc aatctcaaat ccaataccat
     9301 agacagcacc ggtgactaat gctacttttc catctaaacg aaacatatcc atactaaatt
     9361 ccatcatttt ctctccctta tgcttatttt aattgatcca tttgcaccat atccatgtct
     9421 gtataggtga tattttctcc gcacattgcc cagatgaatg agtaattgct tgtacctaca
     9481 cctgaatgga tcgaccagct tggcgaaata atggcttgtt cattgtctac aactaaatgt
     9541 tttgtttcat ctggtttgcc catcatgtgg aacacacgtg tatcttcatt ggcataatca
     9601 aagtagacat aagcttccat acgacgttca tgtgtatgac aaggcatcgt attccaagcg
     9661 cttcctggtt ctaaaatcgt ataacccatt tgtaattgac agctttcgca tacatttgga
     9721 tggatgtatt ggtagatttt acgttcattc agtgtcaggc cttcaccagt ctccattggt
     9781 tttacttggt cgatactgat ttttacattt ggatatttat gatgagctgg aacagaacta
     9841 atatagaatt tagctgggtt ttcagcatct tgagaagaaa atacaacatg tcgtgtttct
     9901 tttccgatat agtatccgtc ttgttttttc atttcttctt tttgcccatc gatctcgatg
     9961 aatcctggtc caccgatatt gatcacacct agttcacgac gctctaagaa gtaatcaaca
    10021 cctagttctt tgtctaatgt gatttctaat tctttttcag taggtgtcac tccaccaaag
    10081 atcattcgat cgttgtgcgt ataagtcaaa ctgattttcc ctggttcaaa taccttttct
    10141 actaaaaatt cttttctcaa ttgttccgtt gaataatgac ggatatcttc tggactgtgt
    10201 gtataacgtg tttccatttc ttgcattttc ttctactcct tctatcgaat gatttttcca
    10261 gagccgcatg ctaaaaattg tgcgacttct tcttgactaa attggttgca gtccccatgg
    10321 atcgtatgct tcaaggcaga agctgccgta gcaaaatcaa tgatttcttg cggagacata
    10381 ttggataaaa tcccatggag cacgccacct gcaaaagcat ctccgccacc tactcgatcc
    10441 acgattgggg tgattctatg gacttgggat tccacatatt catggttcat ccacaatgtc
    10501 cccttcaatt cattttcact tgctgaatga acggtacgct tggtcgaata aaacacctga
    10561 atattcggga acttttcctg cattttttga taataataaa ttagaggttg ttcttctgct
    10621 tctggcgctt tctcgatacc taacaaataa agagcatcca tttttccagc tgagcagata
    10681 tctactaaag gtaaaagctg atggattact tcgctggctt cttgctgtgt ccataatttt
    10741 ccacgatagt tgatatcgaa gctgatcagg cagccagctt tttttgcagc ttctatcacc
    10801 tgtttcgtca ttttttgcca ttttttagac aaagctgggg tgattcctga aacatgaaat
    10861 atatctattc cttggaataa ctcctctagc gaccattcca aatgatccat ttcagcaaaa
    10921 cttgatccgg cacgatcata gatcactgag gcggcgcgtt cgccactacc agtttccata
    10981 taataagtac ctagtcttga tccaccagat aatacctgtt tcgtatctac tccataacat
    11041 tgcaagtgct ttttgactgc ttttcccaat gcgttatcag gtacttttgt agcaaaaatc
    11101 gcttcatgtc cgtaattagc taaagaaatt gctacgtttg cttcccctcc gccataatgg
    11161 gcagcgaatc gttcacttga atcgatacgg gttccctgct ctgtggacaa tcgcaacatg
    11221 atttctccga gtgtcactac ttttcccata agcagctacc tcttgatttc tttcattttt
    11281 gctgcatact gttgcgcgac ttccgttact ttgtcaaaat ctcctgtagc tgcgggtgct
    11341 aaaaggttcc cacctactcc gactgttacg acaccagcat cgaaccactc tttcatattg
    11401 tccagactca caccgccagt tggcatgata ttgacttgag gcatcggcgc cttgaaggca
    11461 gaaatgatac ttggtccata agcgcttcca ggaaatagtt tgacgatatc taccccgctt
    11521 ttcagcgctg ttttgatctc ggtaatagtc atacatcctg gtaagtaagg aatttgatac
    11581 agattgcaca tctctgccgt ttcttgatca aaggaagggc taacaatata ctctgcgcca
    11641 gctaagatag ccaagcgagc tgtgacagcg tctaatactg tgcctgcgcc aatcactatt
    11701 tctggttggt ctttgtagaa tgacaaaagt tctttgatta cttgatctgc ttgagggact
    11761 gtaaaagtca actcaatacc agttagacca cctttgatga ttgcgtggca agcattcaaa
    11821 gcttcttctt tgctttttcc gcgtaccacc gcaatcactc cggcgttttt caatcgagat
    11881 aaaatatcaa tttttttcat cttctccacc tatttcctat ttagaaattt tattttataa
    11941 cttggaaatc tatacccata ttatacggca gaaaagaaaa attgcaaacg cttttttcat
    12001 tatttttgaa ttctttgctc gatttcgctt tttgtttgct tgagggcact gattatttgt
    12061 tcttttgtct catctgttaa acgatatttc ggcatactta cactaaatgc accaatgatt
    12121 tgttcgtttt tcacgattga ggctcctata caaaaaatat cacgttcgat ttcttcatcg
    12181 tcaaatgcga tgtgggtttc ttttaccgtt ttgatttcct ttttcaaccg taatgcattg
    12241 gtgatcgtat attctgtgta agggatcaac ggatgctttt caagataatc agtgtactgc
    12301 tcttcactga attctgctaa tacggctttg cccatcgcag aattgtataa tggtcgggtg
    12361 attccgattt ttgaagacat gcggatcgtt tgatgttttg gatctagttt gttcacgtac
    12421 aagatctcat tgttgtccaa tacccctaag tggatagtct catcgatctt ttgctgcagc
    12481 tgttctaaaa acggtaaagt caactctacc aaatcgattt gttcaatgtt tttattcgcg
    12541 tagcgaataa gctttgcacc taaacgatag tttttctctg aatttttatt tacgtagccg
    12601 atcatcaaca aagtatctag tattttcaag actgtagaag aggtcattcc cacacccttt
    12661 gcgatcgtct gcaaagagac atcttggttt tccgctaaat agtctagtat tttagaggct
    12721 ttgatcaata cagttccata aggcttttta ttttccatat aatattctcc tatttcttta
    12781 taagaaaaat tatagcggaa ataccatcaa tttaaaagag atatccagat ataaactacc
    12841 atttctcttt acaggcaatt taatgttgaa gatccgcttc gtttttacat caaactttga
    12901 tataaagcaa tgatttcttc tactgtcgtt tctcttggat ttcctggtgt gcatacatct
    12961 cgcaaagcat cttctgcaat tgctggaata tctgcttctt ttacacctaa ttcagaaatg
    13021 gtttctggga ttccaaccgc tttgctcaac cgatcgatct caccacaagc cgcttctctt
    13081 acatcttcta aagacatttc agctgctcct ttaatcccta gaacaagagc gatctcacgg
    13141 tacttttctc cagtatattc tttgttatat ttcatgacag ttggcaaaag tgctgcacac
    13201 gctacgccat gaggaatgtt gtaccatgca cttaacgggt gagccatccc gtgtaccagt
    13261 cctaaaccga catttgagaa gcccattccc gcaatatact gtcctaatgc cattttttct
    13321 cgcccatttt gatcgccatc tacagaagct tccaaagaat ggccgatgat ttcgattgct
    13381 tttaaatgca gcatatcagt catttcccaa gcgccttttg tgataaaccc ttcaatagca
    13441 tgcgtcatag catccatacc tgttgatgca gccaattttt tcggcattcc catcatcata
    13501 tcgctgtcga tgaacgcgac gattgggata tcatgaggat caacacaaac aaattttcgg
    13561 tgatttactt catctgtgat gacgtagttg atcgttactt ctgctgctgt tccagaagtc
    13621 gttggtatag ctaatatagg taaacatgga tttttcgtat cagcaacacc ttctaaacta
    13681 atgacgtctg aaaactctgg attcgtcaaa ataatcccaa tggcttttgc cgtatcgatt
    13741 ggcgaacctc caccgattgc gatcaaacag tctgcctctg cctctttaac aaaagccact
    13801 ccatctttta cattttggat cgttggatta ggtacgatcc cgtcgtataa agcataagcg
    13861 attgctgctt tatctagaag ttctgttact tttgttgcta taccgtgctc gatcaatcct
    13921 ttgtcagtaa tgacagctgc tttgccgaat cctcgctgct gaaattcagg gacgatcgac
    13981 tcaatcgctc cttttccaaa atacgatgtt tcatttaaaa tcattcgatt actcattttc
    14041 tattcctcct aatttgtatt atttttcaca ataaaaacga aagaacccca agcaaccggt
    14101 tgcccagttg cttgttgcta cagttcaaag actggcatca attcccaact gactggagag
    14161 gcatctggat ttgtctccat gatatcttcc atgtacttcc accatttttg attgatagat
    14221 gtatttgcca ttttttccca taacttttca tcttcgatca ctaagtaacc aaataacaaa
    14281 cttgtactcg gatcaagata gatcgaatag tcgatgaccc cgtgttcacg aagtgctgta
    14341 cgcatttctg gccaaagttc atcatgtctt tttttgtatt cttcttgttt ttctgggtaa
    14401 actttcatgc aaaaagcttt tttcaccatg tcagacacct gctttcgtat gaacttcctc
    14461 caaataacct gcttttggag tgacaccaaa cgcatctgct aaatgccaca ggttttcgtc
    14521 cgtgattgtt tgcaagattg gtccttgcgc tttgacgtac gtatagactt ctgctgcctt
    14581 ttctgctgtc tcgatcaaac caaagacttc atccatgtct tttcctgacc cgtagattcc
    14641 atgttgtggc cataaaacca atcgagtttc tttcattttt tcagctgttg cttccccaat
    14701 ctcatttgtt cccggaacca tccacggaat gatggaaatt ccttctggaa aaacaactaa
    14761 gcattccgta cacatttgcc atagcgtacg actaaaggaa cgttcgtcta attcatgcgt
    14821 aaaactcatc gctaatagat gactagcatg gcaatgcata acgatccgat tatctgggtc
    14881 tacttctaaa cgggcgatat gactcatgaa atgacttggc aactcgcttg taggaaggca
    14941 ttcgttttcc agcccccaca aaagttccag tgttttgcca tcttcagcaa ctcgaacgat
    15001 tccaagattc tcggcaggtt cgctcgctac atttttaaaa tatcttcctg aaccagttac
    15061 aataaaatag catcctgcta aacggctggc atcaaaaatc atcgagatgc ggcggatcac
    15121 tttttcagga tccaagaacg gtgtgatctc ttcttctttt agtaaataag aaatattccc
    15181 gccatttctt tcatcccatc ccaagcgata aagattcgac gtcgtttcga tcatttcttt
    15241 cacataaggc gcttgtaaaa tatctatttt tttcaatata gccacactcc tattttctta
    15301 attccaacac gtctttttga tattcctgga cttcttccag ccaatccaaa ccaactggaa
    15361 cttgattttt gtaacagaaa taattccata catcggcata cgggaaatct ttcaattctt
    15421 ccgtataagc cattcttgaa gtaaagtcaa aagccagctc catttctttc aatcgctcaa
    15481 ctggttccag catagctttc agcagtgctt tttgtgtatt tctcgtaccg atgacccaag
    15541 cagcgatacg gttgatcgtt gcatcaaaga aatccagacc gatatttgtt ttttccaaca
    15601 attcgttacg gactaattct ttagcgattt cctgcaattc atcatccatc aaaaccacat
    15661 ggtctgaatc ccaacgcact ggacgggaaa catgcaacat gattcctttg ctgaataaag
    15721 ctaatgaaga taatttattg gaaatcactt ctgttggatg aaaatgtcca gcatctagac
    15781 aaacaagttt gtcacgagtc aacccgtaac ccatataaaa ttcatgggaa ccgactgtat
    15841 aagcttcagc gcctaaacca aatagtttgc tttctactgc atcttgtaca taccgttcat
    15901 ctaactcttc tgtaaaaatt tcatcgagtg attccattaa acgttcccgt ggggtaaaac
    15961 gatcaatcgg attatctttc atcccatcag gtacccaaaa gttattgata cttgtttgcc
    16021 cagtttcttt cccaaagtac tccgcgattt tgcggcttct tttcccgtgt tcgatccagt
    16081 attcacgaac ttctttatct ggatgagcca atgtaaagcc gtctttcatc attggatgcg
    16141 aaaagaaagt cggattgaag tcaagaccga tttcttcttt ttttgcccac tctacccatt
    16201 tttcaaaatg tttcggctca atttcattta gatcgacaga ttcttctgta tccaaataga
    16261 tagcatgaag gttcaatttg tgtttgcccg gtatcaacga ataagccttc tccagatctt
    16321 gtctaagctc ttcaggtgtt cgtgcggctc ctggaaaatt ccctgtcgcc atgattccac
    16381 cggtcaattc gccatccggt gtcaagaaac ctttgacatc atctccttgc cagcaatgca
    16441 tagaaatttt gatgtcctgt atcttttcta gtacagcatc tgtatctaca ccgatagttg
    16501 catatttttc ttttgcttgt tcgtaatttc tttcaatcgt gttcatacga ttactccttt
    16561 gattttattc acgtaccatt ccatcgtgtt ttgcttattt tcatctgggt aaaaagtttc
    16621 acatggaaac gactgtttga ttacatttct tgcttgttct atattcgaaa actgacctgt
    16681 actgagcatt tgcatggcaa cattcccgat tgctgtggct tcactaggtc ctgcttctac
    16741 aatgattccg gcataatctg ctgtcaattg attcagtaga cgattattcg atccgccccc
    16801 tacgatgtgc agcttttcca atttcgttcc agttaagtgt tctactttga tcagctcttg
    16861 tgcgtaacaa agagcaagat tatcatacac acaacgagcg atagctcccg atgagacagg
    16921 gactggctgc tctgtttcaa aacagtattg ctgaatctct tctatcatat ttgggggatt
    16981 caaaaatcga gggtcattga tttccatcac ttgctgcagc ggtttttctt ttgcagcaag
    17041 ctctgccaat tccgcataac tgtactcgta ctcttgcatt cgggcaactt cctggatcaa
    17101 ccacatcccc atgatgtttt tcaaaaaacg caccgtgtta tgggcacccc attcattcgt
    17161 ataattttca gaaaaagcat tgcgagaaac attggcgatc tttgtttcaa tccctaaaag
    17221 cgaccatgtc ccactgctga tataacccca acgttctcct tgacctggcg ttcctaatac
    17281 agctgacgcc gtatcatgac ttgctactgt gataaaggtc acatctggca aatcatattt
    17341 tgaaaattgt tctttttgca gatctcctag tatcgttccc ggctctgtca atggtggaaa
    17401 taattccttc gagacaccga ttttttcaag aagttctatt tcccattctt ttgtccctgc
    17461 attcagcagc tgcatagtag atgcattagt tttctccgtc accatgttac cagtaaagac
    17521 ataccctaaa taatcaggta tcagtaacaa tttatgtgct tttgccaaat tttctttttc
    17581 ttcagcaaac aattggaaca aggtattgaa aggctggatc tgaatccctg tctgctcata
    17641 caacttatct agagaataga tttcagaaaa ctgttctact gctccattgg tacgcttgtc
    17701 ccgataagca atcggatcat ttatccgatc cccttgttca tcaatcagac agtaatcgac
    17761 tgcccatgta tcgatccccg caaaaaggcg atcaatccct gtctgcttta atttttcaaa
    17821 tccaatcaaa atctctttga taagataatc gatcttccag cgatcgtgac cgtctttgtt
    17881 ccgaaagccg tttttgaacc ggtgtacttc ttccaagatc aaagaacctt ttgcatccct
    17941 cgtacttttc attagtcgtc cgcttgatgc accgatatcg atggcaacaa atgttggatt
    18001 catttctcca cctcttttac tttattgttg attagcactt tattaataaa gcgttatcat
    18061 ttattgacaa tgctatcata atacagaatc ttatattttg cttcacacta ttttggtata
    18121 taattgcatt atcttgacaa gttgggagtg tttacatgga ttactgggaa aaagccaacc
    18181 actcatggtc ggaagactca aaaagagtga tcttgacgcc tacacaaaga agcaggaatt
    18241 tattttttta tatccaagaa atcggttact tcaaagcacc aatcccttac tatactgaac
    18301 gagcacattt accttcttac ttgataaagt ttactttgtc tggcaaaggt tcgcttacat
    18361 ataatgataa tcagcaacag ctaaaagctg gagacttatt ttttattgac tgccaagaat
    18421 atcagcgtta tcaaacgatc agcgaagaac catgggaaat ggattggatc catttgaatg
    18481 gtcagaacgt ccaatcattt tatcaggagt tcacaaaaga cggccataac accttccatt
    18541 caacaggaga tccatcagaa aacaagatcc accatttgat cacagaattg atccacatgc
    18601 aacaaggcac gaatgcccgt accgatttcc agtcctctgt agctatccac gagttgctga
    18661 atgaactgat tttgcaaaag tatcagctag atttcgaaga agaggatatt cccgcctacg
    18721 tttacaaact gaagcaatac ttagacgaac atttcaaaga aacgattaca cttgatcaat
    18781 tagaacaaca attcttattg aataaatatc agctgagcaa agatttttcc aaatttatcg
    18841 gttctccacc aatcgattat ttgatcagca aaaaactttc ttacgctaaa gaccttcttc
    18901 ggtatacaga atttacggtt cgaatgattt ccgaagaaat agggattgag aattttgctt
    18961 actttagccg cctgttcaaa gcaaaaacag gactatcgcc gagagacttt cgtaaattca
    19021 gctgattcat ataatatgta cgtcattttt tcaaaatatt ttgaaatata tagataaaaa
    19081 taaaataacc gcagtttctg cttctatgta aacaacagca gctactgcgg ttatttttta
    19141 gtgatcattc tcattgttca tcttcaaaat gttcataatg aaccaactgt ttctgtgtat
    19201 cacgtttcaa aaattcttta ggcgttatgc cgaactctct cttgaatgct ttcagaaaat
    19261 gcggataaga agaaaaacca cttttactat atacagcact agagctgcgg tattctcgca
    19321 acaagtgctt ggcatagatc agctttttct tcattacata ctgatgaaac gtaaatccag
    19381 tgtacttttt aaattctcgt gtgatatggt atttgctgac aaaaaactgc ttttccattt
    19441 cctctaaaga aagcggatga gataaatttt ttgagatata ttgtaacatt tgttccacac
    19501 gctcattagg aactacagca ttctgttctt gcgcactctc ttctttttgt acaacttgat
    19561 tgagaaaaat caagtattct accagagctt gttcatattt tatgtcttca ccgtaaattt
    19621 ttttatcagg aggatggtcg ataaatgata atttttgctt caatacttcc tgttctattt
    19681 gcaatacacg actcctcttc tctcccattg gttgaaaaca agccgataga ttagaccatg
    19741 gcgtggaacg actttctaga aaagaagggg tgacaaagat atagacacgc tcgaatacat
    19801 ctgaagattg gcggacgata cgatgcaaat cattggaatg gatgagcagc acacttcctg
    19861 ctttgatcgt aaattgtcgt ccgttcaaat aaaaagtcgc ttctcccttt aatgtagcat
    19921 ggatctcata gaaatcatga tgatgataga tcgttttatt ttggctgacg acatctttga
    19981 catgatagat ttcatactga tcagaaacca tcgtattgac ttgttcaccg ctgattttct
    20041 tttggagcat gtgccgcacc tccttttgtt cacttatgca caattttatt ataccacgtt
    20101 ttttgtaatc aaaaagagct gcttttatat actcttataa aaaagaagag acgtggcatc
    20161 ttctagcccc gccccctcca ttctaaatat tagttaacca acgtcttctt tacattattc
    20221 aagaagactt cttccataac gaatcccatc atcttttgtt tcttcaagaa ccacatacag
    20281 gtacggacga ttcttcatga tgatttcttt gatttttgtg tactcgatca caccgtctcc
    20341 caaatttacc ggcacgattt tttcctgttc gacacgaaaa tctttcaaat gaaccgcaca
    20401 tatcttttca ccaaaacgtt caaaagcttc ttctaccaat tggacttgtt cttgataatc
    20461 actacttgtg atcaaattgg tcggatctaa aatgattcct agatagtccg aatctactgc
    20521 ttggatcagc tgctctaccc ttgctaaaga atacaccgga tgattcaaac ctggttctat
    20581 ccccaccatc atttggtatt tttctccagc ttttaccaag cgacggataa cggacacagc
    20641 ttcagcaaat gcttcatctg tgaaattttc ttccgtatat tggatttccg gcaaaacaca
    20701 tccagtttcc gaagcaacca tcgacgcacc aaaggaagaa gcatagcgaa gatactgctc
    20761 gaactttgtc agcaactctt gtcgtatcgt taaatctgga tgaatcatat tgatatagca
    20821 acttaaaatt cctacggtca cttcttgctt ttctaaaact cgtttgacat aatttcccat
    20881 cccagaattg atctcatttg cgccagaagg tatttcagga aacgaaagac ctaaggcgag
    20941 ctgcagtgta tgaatccctt gttcttttgt tttttctgca agatcttcta tcgtttggac
    21001 ttcggaaaga tcatgtcctc taattgtaat gttcaacata actattctcc taactcagca
    21061 tattgggtag ccacatgacg atttgcggga agtaagtaat caaaaacaag acgactaaga
    21121 taaccccata aaaaggaaga agcggtttca gcattgcctc tgcttttacg cctcctacac
    21181 ttgcaccgac atatagtcct gtacctactg gcggcgtgat ggagcctaca cacagattca
    21241 tgattgaaaa caacccgtaa tgtacaggat caacccctac acttgtgacg attggtaaaa
    21301 atattggtgt aaaaatcaag atagcaggcg ccacatccat aaacataccc accagtaata
    21361 acacaagatt gactaataac agaataataa atttattatc cgttacgccc ataatcaacg
    21421 aactaattgc ctgaggaata ccggtaaacg ccatggcgaa agacatcatg gaagaagtag
    21481 ccaataagaa catgatcgtc cctgacattg ctactgattg aaacaacata ttaggtatat
    21541 cttttagttt taccgtttta tagtaaaaca tagcaatcaa cagggagtaa atgacagcta
    21601 tcgctgaagc ttctactgct gtaaatatcc ccgtcaagat accaccgata atgatgacaa
    21661 tcaatagaag gctaggaatc gcctctttta ttatttttcc agcatgagcc atctgtcctt
    21721 tttcgacaac agggtattta ttctttttag caacgatgaa tgctactagc atgattccta
    21781 gcgcccacaa agcgcctacg agataacctc ccataaacaa agcagagatc gaagcaccac
    21841 cagcaacaag tgaatacatg ataaaagcag tacttggagg aatgaccata cctgtcggag
    21901 ctgacgcgat attgaccgct gtcgcaaact tccggtcata tccttcttcc acttgctgag
    21961 ggatcaacac tccgccaatc gcggtagaag cggcaatggc cgagctagag atggaaccaa
    22021 ataaagcatt ccctaacaca ttcgtatgag ccaaagcccc cggcacgcgc ccgacgaaca
    22081 acatcgcaaa atccactagt ttcttagcta tccctccatt attcatgata atgcccgaga
    22141 gaacgaaaaa cggaatagcg atcagcgaaa aactattgat acttcccacc attttttgag
    22201 ctgaactaaa agcagaaaca tcaaacggta taactaacaa taaagtcatc atcgatgaga
    22261 atgcaatact tatagcaatc ggcatcccta atacaagcaa cacaacaaat acaaaaaata
    22321 acactaatcc ggcttctatt gccattttat tcctccattt ttatattttt tcttgaattg
    22381 ctggcttgtt gcttaatgta tcgattagat tgatcaggct atagataatg atgagtaccc
    22441 ccgatacagg tactgacaga taaacatatc ccataggaat acttaaagaa ggcgagatct
    22501 gccccattgt caatgtgaca gcattccatc cgccatacac catgatcatc gcagcaaata
    22561 ccagaaataa actttgaaca acaacttcta aaattttact ttttttgcca gttaattttt
    22621 cacttaaaac cgttacggct aaatgtgctt tttgtcccac aacatacgcg gaagacaaca
    22681 tggcaaacca gacaagaccg aatcgaacaa gctcttccgt gaccgtattt ggatttccta
    22741 aaaccgttct ggaaaatact tgccataaaa caacagcgac catcccaaca agcaaaacag
    22801 ctcccaggat ctctaatacc ttgtttaaaa tcattttcat cacagctacg ctccttgctc
    22861 ttggatcttt tgataaaggt ctttgtaggt aggatcgttt ttgaatatat catgaagtgg
    22921 ggcaactgcc tctcgaaacg gtgtattatc tacatcattg aattggactc catgttcttc
    22981 ttgtgctaaa gctttttctt cttcaattgc ttttgcaaaa gcttcaattt caaaaaccgt
    23041 tgattctttc gctgcttctt caatggcttt ttcctgctca tcagttagct gttctttgat
    23101 ggcatcattc atgatgatga tatctggcac acgtgtatgc tcatcatagg aatagtattt
    23161 agccacacca ccatgtccag ctgtatacaa gacaaattca ttgttttctg atccgttgat
    23221 caaattggat tgaagagacg tgtacacttc atcacttccc ataggtacgg gcgaaccgcc
    23281 taaaagctct accattttga tagcggtttc gctttgcatc acacggatct tctttccctt
    23341 caaatcttcc ggcgtatgga tcggaccgtc taccatataa aagctgcgct gaccagaatc
    23401 ataataggtc aatccggtaa atcctaaatt tttggtcgat tggtagattg gctccattat
    23461 ttcttcatta tccatcacct taaagaaatg ttcttcatca ttgaatagat aaggaactga
    23521 aaaaatcgaa taatctttag agaaactttc caacgctgag gcacttactt ttgtgaaatc
    23581 tactgctcct gactgtgtca attcgatcag ttcacgttct ccacccagtt gtccatctgg
    23641 gaaatactgg atctgtacgg aaccatctgt tttttctttt actaaacgcc caaattcatt
    23701 cattgcatca attactggct ggctgttgct tgcataagca aagcgcaagg taactatctc
    23761 atttacatct gcatctgcga cagttgccac actagaatta tttccgcagc cgcttagacc
    23821 tagcagacct aggctaagcg aacttgctgc aaacagcagt gatgtttttt tcatatgggt
    23881 ttcctttctt ttaacaataa gagacgagca attctgtcaa acaaagcacc gttaacgact
    23941 gtccataagg cattgctgtg attccgatat tccgataaaa atctaaactg tcccccattc
    24001 ctgtcccgat tgaaacgttt tgaacttctc ctttttcatc gatttgttca agcaatcctt
    24061 ggatagcacg ataagcgact tctttatatt cttgtggtaa ataacgttta tggaccgctt
    24121 tcaaaatacc gtaagcaaat cctgcagttg cagaagattc tacgtaagaa gaaggatcat
    24181 ccaacaacgt atgccataaa ccgctttcag actgataagt ttttaatgca cgcacttgtg
    24241 cttcaagtgt ttcgatcaat aatttgcgca atgcatcttc tttcgataat tctaagattt
    24301 caatgatctc tggaatagca atcgtcaacc agcagtttcc acgagcccaa aatgcttcgg
    24361 catagttatg atttccttca aacgtccagc cgtgatacca caatcctgtt cgtttatcca
    24421 tcaagtattt gatatggatc ataaactggt aacgagcttc ttctaaatat tctgggcgat
    24481 tcaacagttt cccgattttt gccaaaggaa gcactgtcat catcaatgta tcgtcccaca
    24541 actgattctt attttccggt ccatatgtgg catgctgtaa cccgtcttca tttgtccgag
    24601 gcatgtcatt catgacccat tctgcccatt gttctaaata aggcaaatag cgtgaatctt
    24661 tcgtgtcttc atacaagtaa gccatagtca atagcggtgc cattgtattg acgtttttgg
    24721 gcggagcgcc ttcttccata cgatcctcaa accattcatt gaccacttga taagcgcgag
    24781 gattttttgt catctggtaa tttttataaa taccatatag accgacacct tgtggccaat
    24841 tccacactgt ccagcttttg tcatctactt tcagtccatc aaagtccaac aaaaattcac
    24901 cattatcgtc tttgatcgtt gtcaggtttt caacgagcag gtcaattttt tctttcacta
    24961 attctttagg tacaaaaatc tcttctttaa taatcatctt tacgccacct ttttaaacgc
    25021 tttcatttta tatccttaat atagcagtaa taccgtccac tataaatatc gctttttgaa
    25081 caaaaactgt ctaaaattga cctaaatgtt aaataacaaa agtaactttc agttacttgg
    25141 aaattaaaaa aagaacttgg agaaacaacg ttttccaagt tctttttcat ctattttctt
    25201 atcttttttc tacgctttct tttgcttatc cgttgtaagc atcaatgatg atctgtttca
    25261 attcagagat caatggttct ttcggattag ctgtcgtaca ttggtcttcg taagctaatt
    25321 cagccatacg gtctacggtt gtatctagtg tttcttgtgt gactccttga gcttttagac
    25381 tcatgttgat gccgacagat tttcctaatt cataaacagc cgttgctaaa gcttcgacta
    25441 attcctctgt tgtttcgcct ttcagaccta agaattttgc gatatctgcg taatctgtat
    25501 ctgcacggaa atagtcatat ttagggaaca tcgcatgttt ttgtggatct tttgcattat
    25561 agcggataat atgcggtaaa agaatcgcat ttgtccgtcc atgagggatc ccgtattccc
    25621 cgccgatttt atgtgcgact gagtgacaaa tacctaggaa tgcgttagca aacgccatac
    25681 cagccattgt agaagcgttg tgcatttttt cacgtgattc catatcaggt gtttttactg
    25741 atttttctaa atagtcaaag actaacttga ttgcttgtag gctcaatcca cgtgtataat
    25801 ctgaagccat gactgaaaca taggattcga ttgcatgtgt taacacatcc atcccggtat
    25861 ctgctgtgac agatgctgga actgacatga cgaattgagg atcgacgatt gcgacatctg
    25921 gcgtcaatgc ataatctgct aacggatatt tcacatgtgt ttcactgtca gtgatgaccg
    25981 caaatggcgt tacttcagaa ccagtccctg atgttgtcgg gatacatacg aattgtgttt
    26041 tttctggttt gtcgatctta tatgtccgtt tacggatatc caagaatttt tgttttgcac
    26101 caaagaaaga tgtgtctggg tgttcataga acatccacat tcccttagct gcgtccattg
    26161 cagaaccgcc tccaagagcg attactgtat ctggcttgaa gtctgccatc agtttcgttc
    26221 ctgcgtatac ggtatttgtc gatgggttcg gttcaacatc tgagaagact tcgatcttca
    26281 catcattttt acgtcgtgcc aatacttcac gaactgtatc gcagtaaccg aattggacca
    26341 taccagggtc acaaacgatc atgacacgtt caacgttttc cattttctct aagtacaata
    26401 gagagttctt ttcaaagaag atttttggcg gtaatttgaa ccattgcatg ttatttctcc
    26461 gtttcgctac agttttgaca ttgattaagt ttacagcaga gacgttacga gaaacagagt
    26521 tcttaccata agaaccgcat cccaaagtca atgacggaat catttcgtta tagatatcac
    26581 cgatgccgcc ttctgcagat ggtgtattca ctaaaatacg acaagctttc attcgaagac
    26641 cgaatttgac atgcaactct tcgttttctg aatggataac tgctgtatgt cctaatcctc
    26701 caagattcaa cataccttca cacaaatcaa acgcatggtc cgtgttgtta gctttgatca
    26761 tggcaagaac tggagaaagt ttttctcttg atagcggata atctgggcct acgccttcca
    26821 attcagcaat cagcattttc gttccttttg gtacactgat tcctgccaat tttgcgattt
    26881 ccatcgcaga atgtccaacg attgatggat tgaccgcata ttttccttcg ttcatcactg
    26941 catcttctaa tttttgcaat tcatctggtt ttacgatata aacttggtgt gcttggaatt
    27001 ctgcttttac tgctgcatag atttctttat ccacgatcac agcttgttca gaagcacaaa
    27061 tcattccatt atcaaatgtt ttcgaaacga tcaaatcgtt cactgcacgt ttaatttttg
    27121 ctgttttttc aatataagct ggtacgttac ctggacctac acctaaagct ggttttcctg
    27181 tagagtaagc agacttgacc attccagctc cacctgtcgc taagacgatc gcgatacctg
    27241 gatggttcat tagcatagaa gtcgcttcga tcgaaggatg ttcgatccat tggatacaat
    27301 tttcaggagc tccggcagcg atagcagcat cacgtaccac tcgtgctgct tctgcagaag
    27361 atttttgggc acttggatgg aaagcaaaaa cgattggatt tcttgttttc aacgcgatca
    27421 ttgatttgaa aatcgttgtg gaagtcggat tcgttgttgg agttactccg caaaccacac
    27481 cgactggttc agcaatttca atcaaccctt tttggacatc ttcattgatc acaccaactg
    27541 ttttatcatg tttgatatta tgccaaatat attcggacgc atacatattt ttgattgctt
    27601 tatcttcgta aacaccgcgg cctgtttcct ctacagccat cttcgctaat ggcatatgtt
    27661 gatccaaagc tgccattgcc atttcatgca cgatatggtc tactttttct tgatcaaagc
    27721 tttccatttc tttcaatgct gcatttgctt tttctgccaa atcatcaatc attgcttgta
    27781 cgtcaattgt cttcgttttt tctttttcta ttactttagc catcattatc ctcctaatgg
    27841 gtcatggtta tttgttaaat aattcacatg tatatcatac aatatttatt tttctatgta
    27901 aacacttttt tgtgattttt ttcacagtta tttttaa
//
LOCUS       DAZULY010000009        27644 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000009 DAZULY010000000
VERSION     DAZULY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.009"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(208..2100)
                     /locus_tag="ACG81A_000265"
     CDS             complement(208..2100)
                     /locus_tag="ACG81A_000265"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5013663.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2097..3824)
                     /locus_tag="ACG81A_000266"
     CDS             complement(2097..3824)
                     /locus_tag="ACG81A_000266"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5013664.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3840..4490)
                     /locus_tag="ACG81A_000267"
     CDS             complement(3840..4490)
                     /locus_tag="ACG81A_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFS5013665.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4694..4990
                     /locus_tag="ACG81A_000268"
     CDS             4694..4990
                     /locus_tag="ACG81A_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS5013666.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5162..5782
                     /locus_tag="ACG81A_000269"
     CDS             5162..5782
                     /locus_tag="ACG81A_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS5013667.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5874..7040)
                     /locus_tag="ACG81A_000270"
     CDS             complement(5874..7040)
                     /locus_tag="ACG81A_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5013668.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7052..7405)
                     /locus_tag="ACG81A_000271"
     CDS             complement(7052..7405)
                     /locus_tag="ACG81A_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS5013669.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7533..8555
                     /locus_tag="ACG81A_000272"
     CDS             7533..8555
                     /locus_tag="ACG81A_000272"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFS5013670.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8574..8735
                     /locus_tag="ACG81A_000273"
     CDS             8574..8735
                     /locus_tag="ACG81A_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013671.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(8994..9428)
                     /locus_tag="ACG81A_000274"
     CDS             complement(8994..9428)
                     /locus_tag="ACG81A_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013672.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9538..11397)
                     /locus_tag="ACG81A_000275"
     CDS             complement(9538..11397)
                     /locus_tag="ACG81A_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5013673.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11398..11538
                     /locus_tag="ACG81A_000276"
     CDS             11398..11538
                     /locus_tag="ACG81A_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFS5013674.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11515..12675)
                     /locus_tag="ACG81A_000277"
     CDS             complement(11515..12675)
                     /locus_tag="ACG81A_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5013675.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12982..14406)
                     /gene="araA"
                     /locus_tag="ACG81A_000278"
     CDS             complement(12982..14406)
                     /gene="araA"
                     /locus_tag="ACG81A_000278"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFS5013676.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14422..15120)
                     /locus_tag="ACG81A_000279"
     CDS             complement(14422..15120)
                     /locus_tag="ACG81A_000279"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS5013677.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15077..16738)
                     /locus_tag="ACG81A_000280"
     CDS             complement(15077..16738)
                     /locus_tag="ACG81A_000280"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFS5013678.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16956..18356
                     /locus_tag="ACG81A_000281"
     CDS             16956..18356
                     /locus_tag="ACG81A_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFS5013679.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18608..19687)
                     /locus_tag="ACG81A_000282"
     CDS             complement(18608..19687)
                     /locus_tag="ACG81A_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5013680.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19773..20732)
                     /locus_tag="ACG81A_000283"
     CDS             complement(19773..20732)
                     /locus_tag="ACG81A_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFS5013681.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20744..22255)
                     /locus_tag="ACG81A_000284"
     CDS             complement(20744..22255)
                     /locus_tag="ACG81A_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFS5013682.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22277..22840)
                     /locus_tag="ACG81A_000285"
     CDS             complement(22277..22840)
                     /locus_tag="ACG81A_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5013683.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22846..23709)
                     /locus_tag="ACG81A_000286"
     CDS             complement(22846..23709)
                     /locus_tag="ACG81A_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5013684.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23723..24619)
                     /locus_tag="ACG81A_000287"
     CDS             complement(23723..24619)
                     /locus_tag="ACG81A_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5013685.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24794..26083)
                     /locus_tag="ACG81A_000288"
     CDS             complement(24794..26083)
                     /locus_tag="ACG81A_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5013686.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26055..27572)
                     /locus_tag="ACG81A_000289"
     CDS             complement(26055..27572)
                     /locus_tag="ACG81A_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFS5013687.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT         8074 a         5833 c         4780 g         8957 t
ORIGIN      
        1 ttttgaacac cgtttaagga agaggttgta aaatcagcgg tttgacctga gtattagaga
       61 tacaaaacgg aaaaatagct tctcatattt tgccgttttt tgagctaata ccgaaggaca
      121 gctgattgaa caccgtttat tcggaaaaag ggactgtgac acgacttttg tcacagcccc
      181 taaaaaaaat aataaatttg tactattcta tgaagcttct tcttcagaaa actgactgtt
      241 ataaagatct gcatagaatc cgtctttttc catcaatgta tcgtggtttc ctgtttctac
      301 gatcgaacca tgattcatca caataatgtt gtctgcatcg cgtattgtag acaagcggtg
      361 agcaacaacg aaacttgtcc ggttttccaa taggcggttc atcgcttttt gaatcagtat
      421 ttctgttcgt gtatctacac tagatgttgc ttcatcaagg atcaggacat ctggatttgc
      481 taagaatgcc cgcgcaattg tgattagctg acgctgccct tgcgaaatat tgcttgcatc
      541 ctcgtttaat acagtgttat atccttctgg caatttacga acaaaatcgt ctacgtgagc
      601 agctttagca gcctcaatga tttgttcgtc tgttgcatcg tcgttcccat atttgatatt
      661 gtcatagata cttcctgtaa acagccaagt atcttgcagt accattgaaa actgtgcgcg
      721 caattcatca cgtgaaagat cacgggtatc tacaccatcg taacgaatac tacctccaga
      781 aacatcgtag aaccgttcaa gaaggttgat cagtgtcgtc ttccctgcac cagtcggacc
      841 tacaatagcg accatctcac caggtttgac atccaagtta aaatcagtca ttagcaagtt
      901 atctggtgtg tagccgaatt gaacattttc aaaacgaact ttgtatggtg tatctttttc
      961 ttctggaagg ccagattttt cattggacat ctcttcttca tccaacactt caaatacccg
     1021 ttcggctgaa gcaactgttg attggatagt gttcatcaaa cttgcaagtt gagtgattgg
     1081 ttgagagaac tgtgttgtat attgcaagaa cgcttgaaca tcccctaatg tcatgttccc
     1141 atttgcaact tttactccgc ctagtacagc gacgaacaca tagcctagat ttttaacgaa
     1201 attcatcaac ggcatgatga ttgccgaaat aaattgagct ttccaaccag cggcatacag
     1261 attctgattt tctttttcaa atacttcctg gtctttttca gtatgattga atgttttaac
     1321 aatggtatga ccaccatatg tttcttctac ttggttattc aataaaccta aacttttttg
     1381 ctgatctgcg aaaaacactt gtgatttcgg cgctacgatc atcacgatga tcaagcttaa
     1441 agggatcatc aataacgcga ctaacgtcaa ttgccagctg attgaaagca tcatcacgat
     1501 gaccccaatc aatgtgactg cactagttac aaattgagtc aaattttgct gcagcgtgct
     1561 tgcgatgtta tccatatcat tgattgctcg agacatgata tccccatttg tatgtgtatc
     1621 ataataggaa atcggtacac gattcatttt ggcttccaaa tcatgacgta attcataaac
     1681 tgttcgttgg gaaacacgtg tcataataaa ttgctgtaaa aagttgaata cagcagaaat
     1741 caaatacatc acgattactg tcgcaatgat ctgcgcgatt ttatcaaagt ctatcggaaa
     1801 ttttgttact tgttggcctt gttgcatctg ttgagcgcca gccattacac ctttgaatat
     1861 ttctgtggtt gctttcccca agacctttgg tgtttggatt tggaagatca cggctgctat
     1921 tgctagtaca aatacagcga tgatcgcaac catacgttta gacatatagc caaacaatcg
     1981 gcgtaccgtt ccccagaaat ttttaggctt ttcacctttt acaccgagat ttctgccagg
     2041 tccaccatgt cctggaccgc ctgctggtcc atgagaggga cgtgtgtttg ttttactcat
     2101 gctaagtcct cctctcttag ttgtgaatga acgatttctt gataagtttc gtttgttgct
     2161 aatagttctt catgtgttcc tttgccaacc agttttcctt catctagcac aaggatcata
     2221 tctgcatcca ttactgtact tactcgttga gcaactaaaa cggtgatact ttctttcata
     2281 ttcgttttca atgcttggcg aagattggca tccgttttga aatccaacgc tgagaaggaa
     2341 tcatcaaaca catagatatc tgctgttttg actaatgccc gtgcaattgc cagacgctgt
     2401 ctttgtccac cagagaagtt tcctcctcct tgttccacat ggctatctaa gccatcttcc
     2461 atctctgaaa caaaatcttt agcttgtgcg atttctaatg ctttccaaat catttcatct
     2521 gttgcatccg gcttaccata ctgcatattg tctcggatcg ttccagagaa cagaactgct
     2581 ttttgaggaa caaagcccat taattctcgc aaattatatt gagtcatgtc actaatgtcc
     2641 gttccattta tttggatcga accagattcg atgtcatata atcgagggat caaattgact
     2701 aaggtcgttt tacctgaacc agtcccccct atgattgcga cgatctcgcc cgacttggct
     2761 tggaaatcaa tatcttccaa tgcaagtttt tctgctccat gatatcgata attgacatga
     2821 ttgaaggcca atgttgcgtt ttttccagca aaagaaactg ttttaggatt ttcagggtca
     2881 tggataccga ttttttcatc caacacttca ttaatacggt cagctgaagc ttgtgcacga
     2941 ggcaccatga taaaaatagc ggaaagcatc ataaagctga tcaagatctg catcgcataa
     3001 gtcataaatg caatcagatt accgacttcc aaagtcatat ctgctatgta gtgtccacca
     3061 aaccaagtga ttgctacgtt tgtaccgctc atgatcaatg tcatcattgg catcatcaat
     3121 gcgacgatcg tattaacttt gattgccgta tttgtataat ctttattcgc taaatcaaaa
     3181 cgattttctt caaagcgtgt cttatcgaat gctcgaatca ctctgacccc tgtcaatcct
     3241 tctcgaaata ccaggtttaa acggtcagtt ttcttttgca tacttttgaa taatggaaca
     3301 gcaaagtaca tgattccgcc gatcaaaata atcattactg ggatgacaac taagaagatt
     3361 tttgtcaact gatgatcttt ttgataagca agtatgctcg cgccaatcaa cgtgattggt
     3421 gcattgatca tcatacgtaa aaacatttgt gtaaccattt ggatttgagt cacatcattg
     3481 gtcgttcttg tgatcaaaga ggccgttcca tatttatcaa acgcatcttt tgtcagactc
     3541 tctgattttt tatagacatc cgtacgcaat tgcttaccca gtttctgtga ttcacgagta
     3601 gcaaaaaatg tatttgcgat agcagctaag atactgatca aagaaaagcc gatcatcaca
     3661 aaacctgtat gccaaatata atcgacatct ccttgcgcta cccctttatc aataatattg
     3721 gaggtaagtg tcggcaaata taaatctgcc agtacttgga tcaccatgaa aactgcagct
     3781 gcaattgctg aagtcaagga gatccgtttt acgattttta gcatctagtt cccttctttc
     3841 tatttatata ctccgttttt cagccaagaa agtttcaaat tgaattcact gattgcttgc
     3901 tctactgagt aatcttcaga tgttgaaaat aagcgatacg aatgcgcaat tgttgtaaat
     3961 acagtattca tcactaattg gatcagtaat tccaattcat gatcattttt tactttcagc
     4021 ttcgtccgat cgatcaattc aagcaactct tttgcatctt gtttgtgttc tttatgtgga
     4081 tcgtgaggcg ttttttggcg actcgtatga ggagcgactt ttcttgaggc acggtaatcc
     4141 atgttcataa acaagttacg ataaaaagaa gcattctcgc cttccaaaat ctcataaatc
     4201 attttagaaa aataggattc gaatcctttg aatagatcac catcggattc tttcattagt
     4261 tgtaacatat ctttagagct gtcacgtctc attgtctcaa agtaataaaa ataaagatcc
     4321 tctttatctt caaaatactg atagaaactg cctctaggga tatccgctaa tttgacgata
     4381 ttagcaatcg aagcttcttt taagggtact ctagaaaact cgattctcgc agcctcgatt
     4441 agtctttttt gtttttcttt aggcaaatgg aaaaatgtct gtgtcggcat atggatcctc
     4501 ctttctttca agatgacaca ttgtcatatg acgctgtgtc attataacac ctcctacctg
     4561 ttttgcaaga catttgcata gtttttttca ttatcatccg atacattaaa aaagtgataa
     4621 gaggaatcct tccatttgaa ttaatgagag ttgcccatta aaatagccac atactaaaca
     4681 aaggatgaga taaatggccc aatcaattag tgtccaagac ttcaataaat tgcctctcga
     4741 tgctgacagc atcgttttag atacaagaaa taccttagat tacacaaaaa accacttaag
     4801 ccactcgctt tctatccccg ctgctatctt gccaagacaa ttaaaaaatc tagatccaaa
     4861 gaaactttat tatattctaa gctatacagg caggcgatca gaagcattag ccgatcaact
     4921 tactgctaat ggttttcgtg ctgtttatat cattggtggg atggaagctc ttcagtcgag
     4981 tgttgcttga atattgcttg ttagattcaa aagaggctgt gacaaaaaat cgtgtcacag
     5041 cctcttttat accctgtctt tttttagtta tttattcttc aaccttatca atatgacttg
     5101 atagcctttt ctttttctca tagttataac aatcatagca ataaaaggag aaggtaagca
     5161 tatggcatct gttttagtag tcaaaggtca tcccctaact gcagaagaat cgcgcacagt
     5221 taaggcattg acgtcttttt taacaagtta taaagaaaat catccagatg atgaagtgac
     5281 tgttttggaa ctttatcgcg atgatattcc ggaaatcgat gaagaattat tatcaggttg
     5341 ggaagcatta cgtgctggtg cagaattcac ttccttatca gaaagtcaac aacaaaaaat
     5401 tgctcgtttc aatgaattga ctgatcagtt ccttgctgct gataaaatcg ttatcgcaaa
     5461 cgccttatgg aatctaaata tcccaacaaa attaaaagct tggttcgata ccgtcaatgt
     5521 agctggaaaa acgttccgct atacagaaaa cgggccagaa ggattagtga caggtaaaaa
     5581 agcactgcac atccaatcta atggcggtgt atacaacgga caagactttg cctctcaata
     5641 tgtaaaaggt attttaaact ttgtaggcat cgatcaagta gatcagctat tcatcgaagg
     5701 aatcgattat gaccctgacc gtgcagatga gttgatgcaa aatgcattag ataaagctgc
     5761 tgctttaggt aaatcatttt aaaaattgag ataaatgcat acaaaagaga cagaggaaaa
     5821 aatcttcaaa gcagcatttg taccttggat attttcccat ctgtctcttt ttgttattct
     5881 ttctgctttt tcaacggatt ttcttcataa agagaagaga ttgttgtgta ccattcaaaa
     5941 acatgagtaa tcactacttt agctgctgca taaacaggaa tccccaagat cacaccgatg
     6001 attccgaaca atttcccaga agtcagcaga actaataaaa tcgttactgg atggattgct
     6061 aactgattcc ctagtactaa tggagagatc aagcgtcctt ctattgtctg ctctactgca
     6121 aaaacgatca gtactttgat gatcatgaat ggacctgcta caatggctaa aaagatagcc
     6181 ggaatcatcg ctaaaaatga tcctaagtaa gggatcaggt tcaaaaaacc cgcaataatc
     6241 cccaacgtga cagcatactc taaaccaatg accgcaaaac caatcataaa catgattgcc
     6301 acagcaaaag ccacagttag ttgtcctctt atataagaag aaacttgact attcatttca
     6361 ctcagcacat tcaacgtcgg tttttgcatg cgtgtcggta aaaatttgac aaaataaggt
     6421 gctaattgtt taccgtcttt caatagataa aataagataa acggcatcgt cagcagagcc
     6481 actaaaatag tcgctaccgc gccgacaaaa cttccaagac tctgaactgt actcttagat
     6541 atatcttgga tcatatcgcc tgccgaattc atgaacttat cacctgctgt ttccaactgt
     6601 tcgcgaaatt ggttgaacag cgggtcccgc aaaatctcgg ttaatttatt atcaatcgta
     6661 tctacatatt gagggaagtt actgatgaag ctcactgttt gttcctgaat cttagggatg
     6721 atcactacgc tcccccatgc gattagtgca acaaccaaaa caaacagccc aatgatactg
     6781 taaagccttg gtacttttcg cttttcaaag taatcaacta ccggattcat caagtaatac
     6841 aagattcctg ctaaaatgat aggcagacct acgattgcta aaaattgcca gattggtgca
     6901 aacaaatatg aaacttttgt aaataaaaag acgatcagca atattaacaa tacaacaagt
     6961 aaggctgtga ctacttgatt atttaaaaac cagcgccaaa accacgatac cggtctgttc
     7021 ttcttatttt ttccttccat aattcctcct gttagttata aaaaatttgg cttcccaaac
     7081 caatagcagg cagtctatgc ggatgaagat agattccatt tgcaatttgt ccttcttctg
     7141 gacaagcatc aaaaatcaat gatacgtgag ctactgtctc taaattccca ttggccatcg
     7201 gaccgacata atcgatcgta tatacctgat catcaaaagc aattgttccg cctgctttca
     7261 aagaaaaagg ttcgtctttt gatacttctt gaatcaccga atattttcgt aaggcagctg
     7321 ttgctgtttt atcgaacaaa atcagcatgg gttcttcctt gtttattgct tctgtcccaa
     7381 tttctgtaat ctttgcctga atcattgcac tctctccttt aattgctttc aaaaacagta
     7441 taacacattc tcattcttta gccgtgtgga ataattatgt gatagaataa aaacgaaaac
     7501 tatttcatta caattaggag gaatcagctc atatgatcga aaagataatt ttaggaacct
     7561 atacgcgtcg cgaaagtaaa ggaatctata cgatcgacct tgatacagaa aaagaagaat
     7621 tagccaacct ttcattagct gttgaagaaa atagccctac ctatgtagca aaaagtaaag
     7681 ctggcaatct ttacacagtt actagtgtgg atggattagg tggtgccggt gcgtacgata
     7741 aggattttca ttttttgaat gcggtaacag agtctggcgc tcctttgtgt tatgtagcgg
     7801 ttgatgaagc acgtcaatta ctatacgggg cgaactatca taaaggtgaa gtaaatgtct
     7861 accatatatt aggagatggc gaactgaaag cagcagattc aatattccat caagaagcga
     7921 caggtcctca taaaaaccaa gatcatgcgc atgtccacta tacggatctg actcctgacc
     7981 aacgattagt cgtatgtgat cttggtacag atcgtgtgta tacttatgat gtgtccgaaa
     8041 acggcaaatt gaatcttgta actaccttca ctgcagagcc aggaacaggc cctcgccatc
     8101 ttgtctttca tccaaatgga caattcgctt atttatttgg tgaattagac agcacagtcc
     8161 gcgtgctttc ttataataaa gaaacaggtg cttttgaaga attacagaaa atatcgacct
     8221 tgccagaaga attcgacggt gaaaatggag gggcagctat ccgcatttca aatgatggca
     8281 agttccttta tgcttctaac cgaggacata actcgattgc tgtattcgca gtttttgaag
     8341 atggttccca cattcaaaac aagcaaatca tctcaacaga aggggatttt ccccgcgatt
     8401 tcgcactaga tccaagcaat aattttgtcg tgtgtgccaa tcagaacacc gataatctga
     8461 cattatatcg ccgtcatcct gaaacaggat tactggaatt actgaaaaaa gatgtctatg
     8521 taccagaatg tgtctgtgtt tattttgatc gttagtaaga aaaggagaat gaaatgaatc
     8581 agcaagacgt attagacaga ctacgggaag aactaaatat tcctttcttt gatgggaaaa
     8641 tcgaagataa agagtatagc gaagaggaat atcagaaact caaagccgat ttacagaact
     8701 actttgaaca atatgtacag aatgtagaaa actaatcagc tttccatact atctgaaaag
     8761 aacatcagga caagactttt ccgtctatcc tgatgttctt ttctacaaat aaaaaaccgc
     8821 cgagatggtt agactcgacg gaaaaggagt atttactcga ttttgagtaa gtacaaggtt
     8881 gttgttggtt ataggtatgt cattatcata aaggattttg atcactctat cataaaggta
     8941 atattacatt tatattagat aactctagtt tttattgtta tcaaagattt cttttatttt
     9001 ttcaacaaca aattagtcat tttttggatg gcagctgtca attggtctgt cgcttggtct
     9061 tgttttgctt cagatgtatt gaatagatct tcatctaccc ctgattcaag atcgatactt
     9121 tggatcattt cttcacagcc tgctacatat gaaagataag ctttttcaaa atttttatga
     9181 atgcccattg cttttgctgg tggacgcaaa gtactgactt tcttaagcat tgcccgatat
     9241 ttttttgttc cttcttcaaa taacaggatg atctcttcaa gagtcgattt gtccagttca
     9301 cttgtttttc cttcatcaat tgcgtttctg atcacttcat atttcggatg cattccttct
     9361 ccgattgctt ctgtttcctt gacgatttga tcgatggact gcacataata gccaatattc
     9421 ggtaacatgt tcgttttcct actttctcca ttgttgttat tatttattct acttgttttt
     9481 atcctcaaat gacaagtact tgaaaaaatt taatggtttc tatacagctc gccattctta
     9541 ttcgtgctca aagaaaccat ctgctaaact atattgtttc cctgcgacaa tgtcatattg
     9601 gatcaattga tattcgtcgt aaatctcttg ttgcgagcga ttgaattgcg cttctttata
     9661 ccactgtccg ttttgatagt acagttctcg ttcaaacgcc ggaagttcct gttctaatga
     9721 taaaagcagc tgataaaagc catttgttgt ctgattcgtc tgttccatta gattcgctgc
     9781 aaaataaaac ggactggtga ttgcatcgtg agtcgtttgc ttttctaatt ctcctttact
     9841 gtcgaacata agaaattgtg tttcatgcaa tgtttgtttg tcattttttg cttggatcgt
     9901 atctgaatag atccctggaa gatgatcccc ccaaaaaaca actaaagtcc ttctagaaag
     9961 attcttcaat tcttccgtga actgcttcag tgccgtgctg ctataggaaa tatcttgcag
    10021 ataattctct aagtcaagtg tcccacttcc atctgaagtt tttgcaggat agtctagatt
    10081 atcatacttt ccatcgtagg gcatatgtgt ctgcatcgtg actaaatgaa tgaactgagg
    10141 tgttttttct tccttgagaa gtgtcattac ttctttgtaa gctgactcat cagaaatata
    10201 tggattattc tctatcgtat ccgtataggt catcgtacgc tcgctgatga actggtcaaa
    10261 tcctaatgtc tgataaacat cctctctttt atacatcgaa gtattgtatg gatggatcgc
    10321 tgtcgtctgg tatgattgtg cgtttaacgc tgaaacgata gaaggaagct gatccatttt
    10381 tggtacaagc atcgtgtaag gggtggtgag ttgcgcatta aataacgcca tagaaaaacc
    10441 agtcaatgct tcaaattcga tatttgctgt tccaccgcca tagttttgag aaagcatatt
    10501 gccactatat gtttgatcag ctacttcata ataatctgcc aatggctcac cagatacttc
    10561 tacgccgttt aatcgactag gatctgagaa actttcactc atcacgaaca cgatgttcgg
    10621 cgactcttcc tctacagttt tatttttttc atcggctagc ttctggtatt tttcagtgat
    10681 ttctttgatc ttttctttcg aatatccttc aggttcatcc attggttcta ccttcagatt
    10741 gtacaaaaac ccaccgataa aaccagtgtt atagtagttc attttctggc tgtaaggtat
    10801 ccatagtgcc gttttattat atgcttttcg caacaaatta tcgggattgt tgaaattact
    10861 gatataccct agcagtccaa ttgccacaag cagtgtcgtt aatcggatca attggaatcg
    10921 ccttcctttt ttcaggcttc gaaacatgta ccagcaaaac aatcctatca cggttcctgc
    10981 ggcagctaag atcactgtaa acagcatagt gccggtcata tctttcagca atccgatttc
    11041 agtgatcatc ttcaaatcgt ctggataaat cggttcttgc cgataactca ttttcatata
    11101 atttgcaaaa cctaatacac cgatggctac gacatacgta agggaaccta aagggacaga
    11161 tcctgctagt gcaatcagaa aaataagaat aatcagcaag acgagacagg ctaagaagaa
    11221 tttttccgta tgccaagaaa aagcaaattt caacgcaagg tctacggata gatcattttg
    11281 acaccactgc aaaaataaat tactaaaaac aacaagaaaa aagatactga tgattgctgc
    11341 tgttcctttt aaaatctttt tctgtttggt cgaaagtctg cttatatatc ttttcaaatg
    11401 cttgctcctt catttctatc aataaaagaa tgttgcttct cttattttag ttgtacgaaa
    11461 ttcgaaagta aagaacggaa agaagaatta atgaaatcca aatattctct ttctttatgg
    11521 cctttctggt ttctttaata agcggacctt tcgtactcca gaaatttttt ctagctgttg
    11581 tttcacttga ttgagctgtt caaaatcagt actgtctatg tcaatcaata tgtagacatg
    11641 ctcattctta tggttacgcg atatatctgc gatcgctagc tcgttctcat tcagcagaga
    11701 aaatatctct gctaatatat tggttttctt ttgataaaat atagtgaatc gaaacggcgc
    11761 ttggaaaaac attcgtgcgg atggataatt gaccgcttct ctaaccgtac cgaacaaaag
    11821 ataatctttt aaggcttcaa ctgccaaacg tccgctgtct gccaaagctt cctgagtcga
    11881 tccccctaga tgaggcaaca taagtatacg gtcattttct aagaactctt cttctggaaa
    11941 atcagtcaag tatttagcca gatgttgttt ttccaatgct tgcataacag catatttatc
    12001 aacaatttca cttcttccta cattgataag tacagcatct tttttcattt tttctatatt
    12061 cgcctgatcg atcaatcctt ttgtatcttc tgtcaacggc agcatgacta cgatgtaatc
    12121 cgaggtagaa agaactgttt ctaaatctgc ttgccgaaca taatccaact gggcatcgcg
    12181 gatagaataa cccaagacat ccatccctaa gctatagcaa cttagcgcta cttttgtgcc
    12241 tatcgctcca agaccaagaa gaccgatggt tttgccttgc aattctcgtc ccacataagc
    12301 acttcttttg ttttctgcct gttctaaaat attaggacct gttagtgttt gtaccatacg
    12361 acttgcttcg attatcggac gggaagataa gagcaaacag cacaaaacaa gctcttttac
    12421 tgcattagca tttacacctg gtgtattcat gacgatcgtc ccgttttcag aagctttttc
    12481 aacgttgatc gtgttcaccc caacaccagc tcttgaaatt gctagcaaat ctggtgtaag
    12541 ccattcatct ttcactttcg tactacgaat aactattcct tcggctggca aatcatctat
    12601 tattttgaaa tattcccctt gtaacttttc tatctcttgt tctggaatag catcaatcaa
    12661 atgaacgtga tacataaaac cccctcctta ctaacaagta tctcgaaatt tctgcaatta
    12721 gacaactgtt taccatttga cttcctagaa gcaaaaagag cagttagagc agagtaggct
    12781 gagtgataaa aataaggcta tgacaagtta ctgccataag ccttattttc gagagcaaaa
    12841 tattgagggt gagatatctt gataaatggt gttccgttag ctgacattga taataagact
    12901 aaatttcaaa aaagttgaaa agcaaatttc gtcaatctct tcttatttct taaaactaag
    12961 tggctttttc acaacctctt tttatctaat aaccactgtt tccaactcaa ctaattttgc
    13021 ccacgtttca atttgatctg ttgttagatt tagcgagaca acggtatgat gaccgccacc
    13081 tgcctgaatc catgaatgga caccttcatg gaaattcggt ttagttttcc ataacacacg
    13141 tgcaacaggt aaattaggtg cttcagtcgt tggttcaaag gcttctactt cattgatcag
    13201 cagtttataa tgcgtaccga aatctgccat cgatacgact accccttctc ctgctttccc
    13261 atcaaataca agacgcgccg gatcttctct atctcccatt gataaaggag aaacaacgat
    13321 tcttggtttc gtcgcagcaa gcgtcggatc tacttccatc atatgggatt ccaagattgc
    13381 ttcttggcca ctagctagct cgtaagtata atcttccatg aatcctgtat ctttccctcg
    13441 agccatgatt ttcagcaacc gatcaatcgc tgcagttttc cagtcgcctt ccccagcaaa
    13501 accgtatcct tctgccatca aacgttgtac agctagtcca ggtagttgtt tcattccata
    13561 caggtcttca aaattagtcg taaaagcgtt atagcctcct tcttctaaga aacggcgaat
    13621 accgatttcc tgctgagctt gtacttttac atgttcctcc catgtctttt cttcataatc
    13681 accatagtcg aaggtataca attccttgta ctcttcaaat aattgatcga cttcttcgtc
    13741 tgatacgcga tcgattactt gtacaagatc tccgatacca aaataatcaa cagtccaacc
    13801 aaattgaatc tgtgcttcca ctttgtctcc ttctgtgaca cccacgtttc tcatgttgtc
    13861 accaaaacga gccactttta ttccaaaact ttcattatag gctactgcaa catccatcca
    13921 atcagcaatc tctttttgga tctcaggtcg tttccagtag ccaacgacga ttttattttg
    13981 tttatttaat cgagcattga tgaacccata ttcgcggtca ccatgagcgg attgatttaa
    14041 attcatgaaa tccatatcga tcgttttcca aggaatgctt tcgttatatt gcgtagctaa
    14101 atgaagcaat ggtttttgta gaagctttgt cccgcggatc cacatttttg ctggtgagaa
    14161 agtatgcatc caagtgatca cacctgctac ttcttctcga taattgacct ctttcatgat
    14221 ttttgtgatc gtatcagcag taactgctaa ttcttgaagg cggattggat aaggcaattg
    14281 gccattttca ttcagttcat cgaccatttc ttgtgcatgt ttctttactt ctcgcagtgc
    14341 ttcttcccca tataaatgct gtgatcctac aacaaaccaa aattcttttt ctccaatatt
    14401 caacataatt agccactcct tttatttgtt ttgtccgtaa tacgcatttt ttccgtgttt
    14461 tcgtaaataa tgtttatcta aaatccgttg aggcagctct tctgaaaatg aattgagctg
    14521 tctggtaaat aaattcatct tgcatacttc atctagtacg actgcgttca ttaccgcact
    14581 ttgcgcatct ttcccccaag taaatggacc gtgtccgtgt aaaagtaccc ctggaattgc
    14641 taagggatca attttgcgtt ctttgaatgt ttctatgata acttttcctg tttcatattc
    14701 gtacccgctg tcgatttcct gttgtgtcaa aaatcgagca caaggtaccg atccataaaa
    14761 agtatctgca tgcgtcgtcc ccatcgcagg aacatccaat cctgcttgtg cccagatcgt
    14821 cgcccatgtc gagtgagtat ggacgattcc tccgatttga ggaaatgaac gatataaaac
    14881 agcatgagta ggcgtatccg aagagggatt gagtttacct tcaatgatat gattgtccaa
    14941 gtccacaacg accatgtcgt ctgctgtcat ctcttcataa ctgactccgc taggtttgat
    15001 aacgaaatat cgtgtttcag gatcaaaagc gctcacattt ccccatgtat atttgacgag
    15061 tccgtgttta ggcaagtcaa gatttgcttg gtatacttct tctttcagtt gttccagcat
    15121 tttctatcac tcctttttct tgcaattgat gaatcgctgt tttttcaatg tcgatcccct
    15181 cacggtaacg tctcataaat atctgatagc ctgctatttc ttctgctgaa ggtgtgatgg
    15241 tgattccttg gtcttgtcca aacacgtctt ctgccaagaa gttttctaaa gacttcccat
    15301 gtcgatccat catataggct gctaataacg cgattcccca cgctccacct tctccggctg
    15361 tgtccatcac tgtaactggt gcctccatag cagatgctag aatccgctgt ccaacttctg
    15421 gtgttttaaa aatcccccca tgccccacta aacgatcaat ttggacattc tcagatttca
    15481 atatatccat tccgattctc attgcaccaa aagcactggc taaatggatc cgcatgaaat
    15541 tcgctaaatc aaaacggcta cctggtgtac gtacaaataa tggccttcct tcatttactc
    15601 ctgtaatgtt ttcaccggaa aaataaccat aagaaagtaa tcccccgcaa tccgtatcgc
    15661 cttctagcgc tttgttgaat aataccgtga ataatttatc tctgcttatc tccataccca
    15721 aagaattggt aaattcttca aaaatactga cccaagcatt gatatccgaa gagcagttat
    15781 tcgtatgaac catgcctact agttctcctg aaggtgtagt gaccatgtcg atttccggat
    15841 ggacattttc tagttctttt tctaatacga tcatagcgaa ggcggaagta ccagccgaaa
    15901 tgttccctgt ccgtttggct acactgttcg tagcgaccat accggttcct gcatcccctt
    15961 ctggcggaca aaccgggatt cctggtctca aatttcccgt agggtcgatc agataagccc
    16021 ctgattcgct taacttgcca gcttcttctc ctgctactag aactttaggc aaaatgtgat
    16081 ccaagtcaat cgctacgtgc gctttttcga ttaactgacc aaaaatattt ttcatttctt
    16141 gatggtaggt tttcgttgaa ggatcgatag gaaacatgcc tgatgcatct cctacgccta
    16201 gtactttctc accagtaagc tgccaatgga cataacctgc aagcgttgtc aaaaaattaa
    16261 tttctttcac atgttcttct ttattcaaaa tagattgata caaatgagcg atactccatc
    16321 tctgcggaat attgtattga aatttttccg ttaatattcg ttctgcttct tcagtgattg
    16381 tatttcgcca agtacgaaat ggtactagta actgttcctt gctatcaaat gctagataac
    16441 cgtgcatcat tgcactgaac ccaatcgacc cgattgtcgt aagattctcg ccatagattt
    16501 cgaatacttc tgctgccatt tttcggtaac tgacgcgcag tcctttccaa atatcttcta
    16561 atgaataggt ccagatacca ttttctagtt tgttttccca ctcataacta ccagcggcaa
    16621 taggagtaaa agatgaatca atcaatacag ctttgatcct agttgatcca aactcaatcc
    16681 ctaaagatgt acgaccatct atgacatcta acatactttg cgcatttact tccttcataa
    16741 tgatttaact ccctccttct cgtcaaacct gatcaaaccg tctttttagt agcgcttaca
    16801 atgattattg tatacttttg tacgtacatg tcaagcggtt atttacaaga atacaataaa
    16861 aaacacttgt tttgtacgta cataagttat aaaatgaaat tgcttacaaa atctgatcaa
    16921 ccgccttctc gaaattctag aaggagagaa tacttatgaa aaaagaaaaa aaagtttcct
    16981 catcgtttat ttattttttt ggcgcatttg gcggcattct atttggatat gatattggtg
    17041 ttatgaccgg cgccttacct tttttacagc atgattgggg tttggcaggt aaagcttctt
    17101 tgataggatg gatcacgtct tcggtcatgt taggtgcgat ccttggcggc tctttatctg
    17161 gtcttctttc tgataagctt ggacgacgaa aaatgattct tctttctgca cttattttta
    17221 tggctggttc tgtcctttct gcatctgccc ctcacaatgg ttcttatttt ctgattgcag
    17281 caaggattct tctaggttta gctgttggcg ctgcatctgc cctagttcct gcttatatgt
    17341 ctgaaatggc tcctgctaga ctaaggggaa gattgtcagg gatcaatcaa gtgatgattg
    17401 cttctggcat gctcctttcc tatgttgcag attatttgct aaaagtgttg cctgaaacga
    17461 tggcttggcg cgtgatgtta gggctcgctg ctgtccccgc gctgatttta tttttcggtg
    17521 ttttagctct accagagtca ccgcgttttc ttatgcaatc tggccgcttg gaagaggcaa
    17581 aaagagtatt gaactatatt cggacaccaa aagaagctga acaggaattc gaacaaatcc
    17641 agctgaatgt caaacaagaa aagacaaccg gaacttcttg gcatactttg tttttagaaa
    17701 aatatcgttc cttagtcatt gccggtattg gtgtagcagt attccagcaa tttcaaggag
    17761 ctaatgctat tttttactat attccgctga tcgtagaaaa agcgactggg catgcagcga
    17821 gcgatgcatt gatgtggccg atcatccaag gaatcatttt agttgctggt tctttgctct
    17881 ttttggttat tgcagacaag ttcaatcggc gaactttatt gaaaataggc ggtagcgtga
    17941 tgggactttc attcatccta ccagctgttt taggaactgt actggatgct catacaaatt
    18001 cgttgttgat tctcttgttt ttatgtattt atgtcgcatt ttattcttgc acatgggctc
    18061 cactgacatg ggtaatcata ggcgaaatat tcccactagc tgtccgcggg cgagcatctg
    18121 gcctagcctc ttcttttaat tggattggtt ctttcctagt tggcttgctt tttcctgtga
    18181 tgactgcttc tatgtctcaa gaaatcgttt tcggcatttt tgggatcatc tgctttttag
    18241 gggttttatt tattcaagag atcgtaccag aaactcgtgg gaaatcttta gaggaaatcg
    18301 aacaaagtgc atctaaaaaa acttatccta aacgtatttc tatccatcat tcttaaagag
    18361 gttgtgacag aagtggtcag cttcaagaaa taagaaggaa ccgcttagtt tccagtcaca
    18421 aggaacaatt tcaaaaaaca gcttctcatg tttgatgaat attgtgactt gtgaccagtt
    18481 ggtaggagaa caccgtttag tggagaggtg gtgaaagaaa cgttcagcct tgagcgatga
    18541 caaaagctct cacaaacatt gcgacgagta accgtaggag caataagagg cggtaaaaac
    18601 ccgtctctta tttttcgttt tctttcactg tttcccgttc aactagtaca ggttcataga
    18661 gaatatttgg ctgtgcctgt cctttttcaa tcgttgtgac gatccaccgt gcggcatctt
    18721 gtcccaactt ttcttttgga tgcgtcaatg ttgtcaattt tttattccct aaatggctta
    18781 aagaagaatc gtctgttcct acgatcgata ggtcatctgg aatatgatag ccattttctg
    18841 tcaacttatc tagcattaaa ctagctattt gatcgttata gcaaatgatg ccatctaaag
    18901 cttgttcttc tttcaaccgt tgtactgttt tttcagcgat tgtttctcgt gtttccgtgg
    18961 tgaaagtaat gatatcttcc gtcaaaaaac gcacattata ctgttcacac gcgctgataa
    19021 aacctttcat cctgtatttt ccttgaagat cgtcgatttt tgtgatgaac aacagacgct
    19081 gatgtccttt ttttatcaaa tattctgctg ccataaaacc gactttcaca tcatctaaac
    19141 aaatataaga ggcatttaac tcttcataag cagcattgat catcaccatc ggaatgcctt
    19201 gttctctcaa agcaacataa agtgctaagt ttgggttata ctgattactt ttcgtaggct
    19261 caacgatcaa accatctacc ccataagcaa tcattttctt caaacactct ttctccaatt
    19321 cgtgatcgtt attcgtactg gctaaaagca atgaataccc agattctcgt aattcatttt
    19381 cgattcctcg gataatcgac ggaaaaatat aatcagagag atatgtggtg atgacaccga
    19441 tcgtttttgt tctgttcaat ggtgtttttt ctttcttata gctgtcatct acatatgtgc
    19501 ccgacccttt ttctttacgc agatagccct catttaccaa cacagcgatt gcttgccgaa
    19561 tcgtatgtct gctgacgtca tatatttctt gcagcttgaa ttctgaggga atggttgtat
    19621 ttacttcgta ttcctcacga aggatctttt gttttatctc ttctgcaata tcttgatatt
    19681 ttgccatttg ccttgcccct ttttatttgt acgtacatat atcataacga gtctgcaagg
    19741 tttattcaag caaaaatcaa ggtttcttgt gctcataagg catacggaac aggttcacca
    19801 aattctggaa atccttgctc attccacgta aagcattgcg cgttcgcgtg ccgattagga
    19861 ttgtctaacg gatccccttt ttggttcttc tctggtcgag catgatagat caaaatatca
    19921 aagcacccgt cttctgaaac cgtaaacgag ttgtgaccag gaccaaattg cttatttttt
    19981 tcagaagaaa cgaatactgg ctccgataat ttatgccatg attcttttaa taaaagattc
    20041 ttgtgctcat cagcccacag caggcccatg gcatagtttt catctgttgc actaccagaa
    20101 tacgtaatga atattttccc attgcgagta aggactgctg gaccttcatt tactgaaaaa
    20161 ccccgttttt cccattcata ctcaggtata gaaaggagga tctgcttccc ctttaacgtc
    20221 caaggattat ccatttcgga tagataaaga ttagaattcc cagtaatttc aggatcttgt
    20281 tgggcccaca cataatatag ctttccttga gaagagaaag ttgttccgtc taaactgaaa
    20341 gtttcccttt tcgtttttat ttgtcccttt tctatccaat ctgtattcat tggatctata
    20401 tgctgatttt caagtacgaa catgcggtga tgatgagatt catcacgtat agttttatga
    20461 ttgcttgcag caaagtaaat ataccatttt ccattgatat aatggacttc aggtgcccag
    20521 atcaattcac tcatcggtcc ttcttcatga gcctgccata caatcaggct tgcagcatgt
    20581 tctaattcat tcagtgactt tgcccttctt aattcaatca cttggtagcc tggaaccgat
    20641 cctgtaaaat aataataacc atctgtatgt ttatataccc aaggatctgc ccgttgccga
    20701 acgataggat tattataaag tgtcttcatc ataacaaatt tcctcacatt ttctttattc
    20761 taattacatt ccaagagtat ttttctatcc ctacatttaa ttcgtcatgt ttttctattg
    20821 aaaaatagtt tatttcaggt tttatttttt ctggttcttc acgtgtattt ttgtccaaca
    20881 gatcgctact ggttaatgtg tagtgctctc cttgatctag caaattgaaa tcatgttcaa
    20941 tcgttatctt ttgttgttca tttgtgcgat tgatcaagaa aatagtcagc tgattgccgt
    21001 tatctacgat cacactgtcc acataaggaa cattttttat cttttcacaa tcataaacag
    21061 ggcaaatgat ttgttgtttc aatacttgac ctttagcata tttcgataag tgttgcatca
    21121 cataataagt tggattcctc catgcttgtc ctttagcatc tgtcagtatt aaaggtattg
    21181 tgtttactaa caatgattga caactaatct ttacgcgatc tgcgtgtcgt aaaatagcca
    21241 gccctaatga accaaataat aatgtatctt ccatagtaaa gtaacaccaa tcgatgggac
    21301 taccgacttc aaaatcccta tgtctttttt ccggttgggc atgtacattc cattcatcaa
    21361 acgctagata cattgttttc tcacttctca aatatccttt tacataatca caagtagcaa
    21421 cgatttcttc aatttgacga tcgaatcgat aagcttttgc aaggtatgtc gctgtatcat
    21481 cacgctcatt tttaggaggt ttagtcaagt cttcttcatc atacttatca atatagttat
    21541 gcaaagctaa ataatctaca ttctcataag cttcttctaa tgcgatacga tcccattcag
    21601 ggtacgatgg taaacgtggc gtagagcttc caactaagat tgtttcaatt gaatcatcca
    21661 ctaacttcat gacttttgag gcttcatttg ctaacctacc atattcataa gcactcttag
    21721 cccccacttg ccatggaccg tctagctcat tccctaaaca ccataattta acgccaaaag
    21781 gctttttttg cccatttttt attcttaaat cactccagaa ggttcctttt ggaaaattac
    21841 aatattctac taaatttttt gcttcatcaa ttccttttgt tcctaaattc accgtgaata
    21901 tcggtttcgc ttgtacatga ctgatccatt taaaaaattc gtggagtcca aaagaatttg
    21961 gttctatacc tcgccatgct aaatcaattt tttttggtcg ttgttctttc ctacctacag
    22021 tatccaccca atcataacca gatgtaaagt ttcctccagg atagcgaatg acacctaatt
    22081 ctaattcctt tacataagag aggacatctt gtctaaaccc gtcaccatca gctgtttcat
    22141 ggcccggttc ataaatccct gtatagacga cacttcccat atgctcaaca aatgagccaa
    22201 acagccgaga actgatctct cctaccgtaa aatcctctct gatttttact ttcacttctt
    22261 cgtccaacag ctttccctac ctttcatttc ctatagtcaa gataatagat acaagtttcc
    22321 atacgccacc agcaaagata aaaataaaag gagggaaaag aaatatgagc agtacaaatc
    22381 ctaataacaa tacgcaacaa agaatatttc tcaaaaaatc aaaaaatact ttccatgcac
    22441 tttgttcaaa aatttgtaaa aatgtgatat ctttgttttc agaaatcaca tatatccagg
    22501 ataaaagaaa catggaataa ataagtaaaa ctacatatcc actaattgaa agtacgattg
    22561 tctgttcttc taataattga ttaacaagaa aataaataat gatcgttaac agactaagga
    22621 acaagttctt accaaaatgt tgctttattc cttgaaagaa aaaataaatg atatttcctg
    22681 atccctcacc tttccattgc gccattacat aaaaaacagc acatgtagca ggcacgatag
    22741 taataacagg caaacacacg attacccaga gaatacctaa taagatgtaa tcagaaacca
    22801 caatcaactg tccataaaat ttagtctcaa ctattttcat cttcctcact ctttcattcc
    22861 agacatagct gttccttgaa caaaatattt ctgtgcaaaa atataaacca ctagtaatgg
    22921 cagcattgaa ataaccgttc cagccattac aggtccataa aaagaaatgt tctgccctgt
    22981 aaataaagct aaccctgctg gtaaagttct catttttgaa ctacttgtca atatcaatgg
    23041 ataaagcaag tcattccaac tattcatcag cgtgaaaatc cctaatgata aaagagtagg
    23101 tttgctcaat ggcaacatga ttttcaaaaa aatattaaac tcacttaatc catcaatgcg
    23161 tgctgcctct tccaaagaat ctggtaaagt aataaaaaag gaacgcatca tgaagatacc
    23221 aaatgcagtt gtcatacgag gaatgatcaa acctgcataa gtatcaagca tgccaagcaa
    23281 attgacttca ataaatagag gaatcatgaa gacttggaat gggatcatca ttgtcagcaa
    23341 aacgaaatag aacaaaactg attttccttt gaaacgcata cgagcaaatg cataccccgc
    23401 aagagaatca aagaatacag aagtgatcac tgtccctcca gcaaaaatca ctgtattttt
    23461 gatgtaatct aataaaggga tcgttgtcca aaccttaata tagttatcta gggtatagct
    23521 atctgcaaat attctaggcg gaaatgagat aatgtctttt tcgtgtttaa aagaagaaat
    23581 aatcagccac aaaaaaggaa acgcaataac caacaatata actagcatac taatcgttcg
    23641 aatgattttt ttaccactaa ctgcttttcg actattcaca gtaactgtaa tcgagctttc
    23701 tggtgccata caggtttccc tcctatttca tttgtttttc tttccgtgac atgtacacat
    23761 tagtggagat agctatagtt gcaacaatga taaacaaaac cgaagacagt gctgatgcat
    23821 agcccaattc ataaggtgct gtaaatcctc gactgtaaat atattgaaca gctgtttctg
    23881 tcttaaattg tgggccacca gcggttgcaa catatacttg gtcaaacact tgcatcgaac
    23941 caatcagatt cgttaaaata caaaaaccta aggagggaac gatctgaggt agcgtaatgt
    24001 taaaaaactg ctgtcgattt gttgcaccat ccatttgagc agattcatac aaagagggag
    24061 agattccctg aatagcagtc aagagaatcg tcatagtaac accaaaattc ttccaaacag
    24121 tcatgatggc taccgtcggc atagcaaggg ttggatcgcg aaaaaattga gggatcggca
    24181 agccgacttg tgaaaacaag taaggaatcg cccctacatt cggatcaagc aacatagaaa
    24241 aaataatcgc aattgacgtc aatgaacaaa taactggaat gtaaaatgtc gaacgacaaa
    24301 aacgattgaa tctggtattt ttagataatg ctactgctgc taataaacca acgataattt
    24361 gtgttggcgt ttcgagtacg gtgaaatata aagaatgaat caacgaatta actgctcgtt
    24421 catcactaaa tatacgtgtg aaattttcaa aacttacaaa atttcttgat gtaaagaaaa
    24481 tattcaaatc tgtaaaacta atagcaaatg ttcctaaaag cggtatgaca gtaaataata
    24541 ataaaactaa aaaagatgga gttaaaaaca aatacgcagc acgatcagga cgattccaat
    24601 atttggtgaa tccgttcata tagtccacct catctttcat tttgataaaa tatacaatct
    24661 tggtttaaac gaatctgaat aagatatccg aacaatcgct gtgatttttg caataacacc
    24721 tatctgcaaa tactaggtcg atcattcaga tatctgtatc ttgccaacag tctatcctgt
    24781 ataaagttct gtactatttt tgcagtaatt gattgatggc atcatctgca tcgttcatga
    24841 gtgtctcagg atcttctcct gctaataatt tttcaatcag tggattgatg atatcgttat
    24901 tgatcgcaga gatttgggta aaacctggta aatatggctc tgcatagtcc atttgtttcg
    24961 ataattcaga tacaatagga tcatttttta cttcttcatc ttttgctact gaatccaagt
    25021 aggcaggaaa accattttcg atactccatt ttttaccaat ttcagttgta ttccaatatt
    25081 tcaaaaactc atagacagct tcttttttat tattatctgt acttgccgga atggcatatc
    25141 ctacgccatc taaaatagct gtttttttgt ctgaatcact cccttgtggt aaggtagtca
    25201 cattataatc aatctcgttt gctttcaatc cgttatttaa ccatggccca ttgatttcaa
    25261 tggctagttg accagcattc attaaattgt ccatttcggc gccagaaatc gattctggac
    25321 cagtcttgtc tacatggatc attttttgga tcgcttttaa cgttttaagg ttctcaggag
    25381 acgcaaattc agacttagta taatcatcgt tcactaattt accgccattt gctaacatcc
    25441 agttatataa cggtgcagtt ccatccttat tgaagacaaa acctgataca tttttactac
    25501 tatcagttaa ttttggtgcc atttctgtca attcttccca agtagtaggt ggttgttcag
    25561 tatctaatcc agcttgttta aacaaatcat tattccaata catatacatg ccttgtacct
    25621 gcatggggac aaaatactgt ttaccttcta cttcacctaa ttcaatagcc gtttctttaa
    25681 aatcagattt atctactcca tcatattccc agaaatcgtc taatggttgt accgctccgt
    25741 tttctacata ctgtgcaaaa tctggataat ttaatgcaat aaagtcaggt gcttttttag
    25801 aagaaatcgc tgtaggaagt ttttcattca aattggccca agtcatgata tccatttcaa
    25861 ttttgatgtt tttatcattt gttttattaa attcattaac gatttcttgc aatatttcac
    25921 catcagatgc agtaaaccca ttccaaaaag tcaaagtcac ttctttatca gaatcagatt
    25981 ctgctgaact tccgccaccg caagctccta aaatacctgt tgcagctacc aacaaagagc
    26041 caattaacca ttttttacct ttcatttttt gttcctcctt catctgataa acgacatctg
    26101 accacattcc aagatagcgg tttcaataaa caattgattt tcccatcttc agcttttaca
    26161 tttgttgaat gacttggttt tacttgttca cttttaggaa gattagttgc ttttgtatca
    26221 aatccactca tttcagaaaa atcaatcact ccctctaatg ataattcaga caagtgaaca
    26281 gttagattca tttcttcttc tccacggtta actgcaaata tgacaagttc tttgtcattt
    26341 attacggcta tactttctaa atacggcact tcttcaaaat cttccgtcgc atagtttgct
    26401 gaacgaattt gtgggcttaa cacctctcct tgtccataca cagcagcttg cataaatgga
    26461 taatagatcg tctgtctcca agcttctccg tttgtttctg tcctgattgg tgcaataacg
    26521 tttaccaatt gagcaagaca agcaatcttt actctatctg catgtttcaa taaagtgatt
    26581 aatagacagc ctaatagtaa tgcatcttca aaattatagt tatcttctag taaaggagga
    26641 gcaatctgcc aaggcgctgc ttgctcatct tgttccgctg aatgatacca aatattccat
    26701 tcatcaaagg acaggttgat ctgcttctta ctatgtttct tcgccttgac tgtatcgcac
    26761 atcgctgtca cttcagaaat gaaccgatcc atgtctaagg aacgtgcaag ataatgagaa
    26821 agatcatttt tttggttgcc ataatattga tgcagagaca aataatctat ttgatcgtac
    26881 gcttgatcaa gaactgttaa ctcccaatca ccaaatgtag gcatttgccg attcgaactt
    26941 ccgcataaaa ccacctcaat tgaatcatct acgagtttca tcgccttagc cgtttcctct
    27001 gctaaccgtc catattcttc cgctgtttta tgtccaatct gccaaggacc gtccatttca
    27061 ttccctaaac accatgtctt aattccaaaa gggctctctt gaccattcga acgccttaaa
    27121 tcactccaat aagttccttt aggaaaatta caatattcga ctaaattacg agctgcatct
    27181 actccacgag tacccagatt gacagccata ttgatttcgg cccccacatg ctgacaccag
    27241 ttataaaatt catgaatccc aacttcattc gtttcaattg tccgccaagc taaatccaac
    27301 cttctaggtc tatttgctat gggcccaata ctgtcttccc aacgaaatcc tgacacaaaa
    27361 tttccaccag gatatctaat catgggaatg ttcaattctt ttaccaagtg aatgacgtct
    27421 tggcgaaatc cacttttatt agctgatgga tgatctggct gataaatccc ttcatatact
    27481 gctcgtccca tatgttcaat aaatgaactg tacaaccgcg gatcaacttt gcctacacgc
    27541 ttgacactac taatcaacat tgtggcttcc ataactgacc tcctttaatt tatacatgaa
    27601 taatacacct cgttgaaagc gttgtcaata atataaatgt taaa
//
LOCUS       DAZULY010000010        27413 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000010 DAZULY010000000
VERSION     DAZULY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.010"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..576)
                     /locus_tag="ACG81A_000290"
     CDS             complement(<1..576)
                     /locus_tag="ACG81A_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5013688.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLC"
     gene            complement(709..1392)
                     /gene="rpiA"
                     /locus_tag="ACG81A_000291"
     CDS             complement(709..1392)
                     /gene="rpiA"
                     /locus_tag="ACG81A_000291"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFS5013689.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            1711..2124
                     /gene="rpsL"
                     /locus_tag="ACG81A_000292"
     CDS             1711..2124
                     /gene="rpsL"
                     /locus_tag="ACG81A_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFS5013690.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            2208..2678
                     /gene="rpsG"
                     /locus_tag="ACG81A_000293"
     CDS             2208..2678
                     /gene="rpsG"
                     /locus_tag="ACG81A_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFS5013691.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            2772..4859
                     /gene="fusA"
                     /locus_tag="ACG81A_000294"
     CDS             2772..4859
                     /gene="fusA"
                     /locus_tag="ACG81A_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFS5013692.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5031..6218
                     /gene="tuf"
                     /locus_tag="ACG81A_000295"
     CDS             5031..6218
                     /gene="tuf"
                     /locus_tag="ACG81A_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS5013693.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            6625..6933
                     /gene="rpsJ"
                     /locus_tag="ACG81A_000296"
     CDS             6625..6933
                     /gene="rpsJ"
                     /locus_tag="ACG81A_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFS5013694.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            6964..7593
                     /gene="rplC"
                     /locus_tag="ACG81A_000297"
     CDS             6964..7593
                     /gene="rplC"
                     /locus_tag="ACG81A_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFS5013695.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            7621..8244
                     /gene="rplD"
                     /locus_tag="ACG81A_000298"
     CDS             7621..8244
                     /gene="rplD"
                     /locus_tag="ACG81A_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFS5013696.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            8244..8534
                     /gene="rplW"
                     /locus_tag="ACG81A_000299"
     CDS             8244..8534
                     /gene="rplW"
                     /locus_tag="ACG81A_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFS5013697.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            8573..9406
                     /gene="rplB"
                     /locus_tag="ACG81A_000300"
     CDS             8573..9406
                     /gene="rplB"
                     /locus_tag="ACG81A_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFS5013698.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            9449..9727
                     /gene="rpsS"
                     /locus_tag="ACG81A_000301"
     CDS             9449..9727
                     /gene="rpsS"
                     /locus_tag="ACG81A_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFS5013699.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            9750..10097
                     /gene="rplV"
                     /locus_tag="ACG81A_000302"
     CDS             9750..10097
                     /gene="rplV"
                     /locus_tag="ACG81A_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFS5013700.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            10111..10767
                     /gene="rpsC"
                     /locus_tag="ACG81A_000303"
     CDS             10111..10767
                     /gene="rpsC"
                     /locus_tag="ACG81A_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFS5013701.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            10770..11204
                     /gene="rplP"
                     /locus_tag="ACG81A_000304"
     CDS             10770..11204
                     /gene="rplP"
                     /locus_tag="ACG81A_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFS5013702.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            11194..11382
                     /gene="rpmC"
                     /locus_tag="ACG81A_000305"
     CDS             11194..11382
                     /gene="rpmC"
                     /locus_tag="ACG81A_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFS5013703.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            11407..11673
                     /gene="rpsQ"
                     /locus_tag="ACG81A_000306"
     CDS             11407..11673
                     /gene="rpsQ"
                     /locus_tag="ACG81A_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFS5013704.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            11731..12099
                     /gene="rplN"
                     /locus_tag="ACG81A_000307"
     CDS             11731..12099
                     /gene="rplN"
                     /locus_tag="ACG81A_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFS5013705.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            12136..12444
                     /gene="rplX"
                     /locus_tag="ACG81A_000308"
     CDS             12136..12444
                     /gene="rplX"
                     /locus_tag="ACG81A_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFS5013706.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            12472..13011
                     /gene="rplE"
                     /locus_tag="ACG81A_000309"
     CDS             12472..13011
                     /gene="rplE"
                     /locus_tag="ACG81A_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFS5013707.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13030..13215
                     /locus_tag="ACG81A_000310"
     CDS             13030..13215
                     /locus_tag="ACG81A_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFS5013708.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            13251..13649
                     /gene="rpsH"
                     /locus_tag="ACG81A_000311"
     CDS             13251..13649
                     /gene="rpsH"
                     /locus_tag="ACG81A_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFS5013709.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            13681..14217
                     /gene="rplF"
                     /locus_tag="ACG81A_000312"
     CDS             13681..14217
                     /gene="rplF"
                     /locus_tag="ACG81A_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFS5013710.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            14378..14734
                     /gene="rplR"
                     /locus_tag="ACG81A_000313"
     CDS             14378..14734
                     /gene="rplR"
                     /locus_tag="ACG81A_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFS5013711.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            14755..15255
                     /gene="rpsE"
                     /locus_tag="ACG81A_000314"
     CDS             14755..15255
                     /gene="rpsE"
                     /locus_tag="ACG81A_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFS5013712.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            15270..15449
                     /gene="rpmD"
                     /locus_tag="ACG81A_000315"
     CDS             15270..15449
                     /gene="rpmD"
                     /locus_tag="ACG81A_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFS5013713.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            15493..15933
                     /gene="rplO"
                     /locus_tag="ACG81A_000316"
     CDS             15493..15933
                     /gene="rplO"
                     /locus_tag="ACG81A_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFS5013714.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            15933..17228
                     /gene="secY"
                     /locus_tag="ACG81A_000317"
     CDS             15933..17228
                     /gene="secY"
                     /locus_tag="ACG81A_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFS5013715.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            17290..17937
                     /locus_tag="ACG81A_000318"
     CDS             17290..17937
                     /locus_tag="ACG81A_000318"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFS5013716.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            18128..18346
                     /gene="infA"
                     /locus_tag="ACG81A_000319"
     CDS             18128..18346
                     /gene="infA"
                     /locus_tag="ACG81A_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFS5013717.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            18379..18495
                     /gene="rpmJ"
                     /locus_tag="ACG81A_000320"
     CDS             18379..18495
                     /gene="rpmJ"
                     /locus_tag="ACG81A_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFS5013718.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            18513..18878
                     /gene="rpsM"
                     /locus_tag="ACG81A_000321"
     CDS             18513..18878
                     /gene="rpsM"
                     /locus_tag="ACG81A_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFS5013719.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            18906..19295
                     /gene="rpsK"
                     /locus_tag="ACG81A_000322"
     CDS             18906..19295
                     /gene="rpsK"
                     /locus_tag="ACG81A_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFS5013720.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            19375..20313
                     /locus_tag="ACG81A_000323"
     CDS             19375..20313
                     /locus_tag="ACG81A_000323"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFS5013721.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            20341..20721
                     /gene="rplQ"
                     /locus_tag="ACG81A_000324"
     CDS             20341..20721
                     /gene="rplQ"
                     /locus_tag="ACG81A_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFS5013722.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            20993..21832
                     /locus_tag="ACG81A_000325"
     CDS             20993..21832
                     /locus_tag="ACG81A_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5013723.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            21808..22677
                     /locus_tag="ACG81A_000326"
     CDS             21808..22677
                     /locus_tag="ACG81A_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5013724.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            22667..23464
                     /locus_tag="ACG81A_000327"
     CDS             22667..23464
                     /locus_tag="ACG81A_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFS5013725.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            23667..24413
                     /gene="truA"
                     /locus_tag="ACG81A_000328"
     CDS             23667..24413
                     /gene="truA"
                     /locus_tag="ACG81A_000328"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS5013726.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(24746..>27413)
                     /locus_tag="ACG81A_000329"
     CDS             complement(24746..>27413)
                     /locus_tag="ACG81A_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFS5013727.1"
                     /translation="KKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSIL
                     ASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFPN
                     QMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIMW
                     LDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLLT
                     NWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDLI
                     MKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASFY
                     LVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAAL
                     FAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMSA
                     EALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSAN
                     DSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYNI
                     SKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNLI
                     NTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQA
                     YLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGTK
                     EISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYDK
                     GWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFILY
                     VWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT         8939 a         5108 c         5854 g         7512 t
ORIGIN      
        1 gcataattgc tcgatttctt tttcaggtaa attgatatat ttataacatg ttccaactga
       61 aacaccgcat tttgaagaaa taaattggat cgtttctttt ttttcataat agagacgtcg
      121 gatttttttg atcgtttttg catcgatttt tggtcttccg ccgatttttc cttttttacg
      181 agcttcatct aatccaacta atgtacgttc tttgatcaat gcgcactcca tgtttgctag
      241 gttttccatt aattggaaat agatttttcc catttcgcgc gtatcgattc cttcatcaag
      301 actaatcaag tgcagcccgc gatctttgaa taattgcgtg agttctgtta attgccgcgt
      361 agatttgcct aatcgatgaa gttggcaaat gactagtgca tcgccttgtt gcatgtcagc
      421 gatcactgac tccaaatgaa cagcagtaga atcattttgt tccacttcat atgtctcatg
      481 gaaaatttct tgacaaccaa agtcagataa ttgatcaacc tgagttgtta aatcaacgtc
      541 atttatggtt gtgcgtgcat aaccaataat tttcatggtg attcctttct agcagtgatt
      601 ttctaactgg atataacagt aaaaatgtag cataaagtac gtcaaaaaga tagttttttc
      661 gcaaattttc cgctgtcttt ttttattgga tgatcaagca gtttttcttt atttacgacg
      721 gattgcgtca agaatctcaa cgcctttttc tgagccgaca actactttag aaaccatgtt
      781 taggaaaagc ccatgttcta ctaccccaac aagttcatct aaataggtag ctaagctttc
      841 tggctgttcg atgacttcta gatgcagatc aatgatgtaa tgaccaccat ctgtggttaa
      901 gacttctccg ttttcgtctg tacgcaagac gggttggaat cttttttctt caaaaatatg
      961 aagtagctgt tgggagccat aagggatcac ttcaactggg agtgggaact tcccaagttt
     1021 atcgactaat ttagaagaat caacgatcca aatcgtttct tttgagtacg tagcgactat
     1081 tttctcgaaa agcaaagcag cgccgccgcc tttgatccct tgaaaatcag cactgatttc
     1141 atcagcaccg tctattgtca aatcgactac ggggacttca tcaatactct ttaacggtat
     1201 tcctaatttt tccgcttgtt cttttgtcgc atttgaagta gtaactcctg tgatacgtaa
     1261 accttcttca cgcatacggc gaccaatctc ttctaccata taataggcgg ttgaaccggt
     1321 cccgagacct acgatcatcc cgtcctttac gaatttcgcg gcttcaatgc cagctaattg
     1381 ttttaaattc atctttgcgt ccaccttttt ttagtttgag catcgtgctt tcctcactat
     1441 tttagcgtga tttacggtaa aaagatacca ttctttttta ttccaataaa gctttttcaa
     1501 aaaaaattgc atctcttgtt gacatgtttt ttcattcgtg atattctaac aaaggtgctt
     1561 tatctacagg tttctgtgaa gaaacgtgct gactgtttat caaagcatat ttgataagga
     1621 tgcagacatt gcatttattt tttatcacaa aaaaagaacc acctggatgt gtggaactag
     1681 acgcaaatcg tgggaaggag gaaacaagga atgcctacaa ttaaccaatt agtacgtaaa
     1741 cctcgtaaat caaaggtgga aaaatctaat tcacctgctt tgaacaaagg atataacagc
     1801 tttaagaaaa ctcaaactaa cgtaaactca cctcaaaaac gtggggtagc aactcgtgtg
     1861 ggtacaatga cacctaaaaa accgaactca gctttacgta aatatgcacg tgttcgtttg
     1921 tcaaacttaa ttgaagttac tgcttatatc ccagggattg gtcataactt acaagaacac
     1981 agtgttgtat tattacgcgg tggtcgtgta aaagacttac caggggtacg ttaccatatc
     2041 gttcgcggtg ctttagatac tgctggtgtt aacgaccgta aacaaagccg ttctaaatac
     2101 ggaactaaaa aacctaaagc ttaattttaa ttattaaaca aatgaaactt gcttctacta
     2161 tatagaagta attactatga acaatatttc ggaaggagga gttacggatg ccacgtaaag
     2221 gtcctgttgc aaaacgtgat gttttaccag atcctattta taactcaaaa ttagtaactc
     2281 gcttgatcaa ccgtgtaatg gttgacggaa aacgcgggat tgcagctaac attatctata
     2341 atgcctttga cttgatcaaa gaatctacag gtaacgatcc attagaagtg tttgaacaag
     2401 caatgaaaaa cgttcaacct gttcttgaag ttaaagcacg tcgtgttggg ggttcaaact
     2461 accaagtacc agttgaagtt cgccctgaac gccgcgttac tctaggtctt cgttgggtag
     2521 taaactacgc tcgcttgcgc ggtgaacaca caatggaaga acgcttagca aaagaaatca
     2581 tggatgctgc aaacaacaca ggtgcttctg ttaaaaaacg tgaagacact cataaaatgg
     2641 ctgatgccaa ccgtgcgttt gctcactatc gctggtaaga ttctctctgt ttggtcggct
     2701 tttataagtc gacctcagat agtgtcctta tttttagcaa ctagatagta aagagaggag
     2761 aaacaaggag aatgaccaga gaattttctt tacaaaacac tcgtaatatc ggtatcatgg
     2821 cccacatcga tgctggtaaa acgactgcta ctgaacgtat cttgtactat actggtaaaa
     2881 tccataaaat tggtgaaact catgaaggag cttcacaaat ggactggatg gaacaagaac
     2941 aagaacgtgg tattacgatc acttctgctg ctactacagc acaatggaag ggtcaccgaa
     3001 tcaacatcat cgacacacca ggacacgtag acttcacagt tgaagttgaa cgttcattgc
     3061 gtgtacttga tggtgctgta actgttcttg atgcacaatc aggtgttgaa cctcaaactg
     3121 aaacagtttg gcgtcaagca acaacttatg gtgtacctcg tatcgtattt gcaaacaaaa
     3181 tggacaagat cggtgctgac ttcttatatt cagtatcaac tttacatgac cgtttgcaag
     3241 caaacgctca cccaatccaa ttaccaattg gtgcggaaga cgacttcaca ggaattatcg
     3301 accttgtaac aatgaaggct gaaatgtata caaacgatct tggaacagag atcgaagaaa
     3361 cagaaattcc tgaagaatac cgcgaattag ctgaagaatg gcgcgaaaaa ttggttgaag
     3421 ctgtagctga aactgatgaa gaacttactt tgaaatactt agaaggtgaa gaaatcactg
     3481 aagctgagtt aaaagaaggt atccgccgcg caacagttaa cgtagaattc tacccagtac
     3541 tatgtggttc agcatttaag aacaaagggg ttcaattact attggacgct gttcttgatt
     3601 acttgccatc accgttagat atcccagcta tcaaagggat tgaccctaaa actgatgaag
     3661 aagtagaacg tcctgcagac gacagcgctc ctttctcagc tttagcattt aaagttatga
     3721 ctgacccatt cgtaggtcgt ttaactttct tccgtgtgta ctctggtgtt ttacaatcag
     3781 gttcatacgt acaaaacgca acaaaaggca aacgtgaacg tgttggtcgt atcctacaga
     3841 tgcatgccaa ctctcgttca gaaatctctg aagtttacgc tggagatatc gctgctgctg
     3901 ttggtttgaa agacacaact actggggaca cactatgtga tgaaaagagt ctagttattc
     3961 ttgaatccat ggaattccca gaaccagtta tccaagtcgc tattgaacct aaatcaaaag
     4021 ctgaccaaga taaaatgggt gtggctttac aaaaactatc tgaagaagat cctactttcc
     4081 gtgctgaaac aaacgtggaa acaggcgaaa caatcatcgc tggtatgggt gaacttcact
     4141 tagatatcat cgttgaccgt atgcgtcgtg aattcaaagt tgaagctaac gtaggtgcgc
     4201 ctcaagtatc ttaccgtgaa actttccgtg ctggcactca agccgaaggt aagtttgtac
     4261 gtcagtctgg tggtaaaggt caatacggtc acgtatggat tgaatttaca ccaaatgaag
     4321 aaggtgctgg tttcgaattt gaaaatgcga tcgtcggtgg ggttgttcct cgtgaataca
     4381 ttccagctgt tgaaactggt ttgaaagatg ctatggaaaa tggtgtctta gctggctatc
     4441 cattagtaga tatcaaagct aaactttacg atggttcata ccatgatgtc gattcaaatg
     4501 aaactgcctt ccgtgttgct gcttctatgg cattacgtgc agcagctaag aaagctaatc
     4561 cagtaatcct tgaaccaatc atggctgtag aagttgttat ccctgaagat tatttaggtg
     4621 atgttatggg tcacgtaact gctcgtcgtg gacgtgttga aggtatggaa gctcgcgctg
     4681 gcggccaaca agttgttcgt gcgatggttc cacttgctga aatgtttggt tatgctacaa
     4741 ctcttcgttc tgctactcaa ggtcgtggta catttacaat gacatttgac cactacgaag
     4801 atgtaccaaa atcagtacaa gaagaaatca ttaagaaaaa cggcggaaaa gctgaataat
     4861 ttcttaaaac cggatagtcc tgcaaagaga ttatccaaag agactatatt tttaatatag
     4921 tttcgtgtaa aataattatc gatgatatgg gcatgtggtg taccttgtgt acatcatata
     4981 cctatcagac aaataaaaaa tatattgtat ttttaggagg acattttaaa atggcaaaag
     5041 aaaaatttga ccgttctaaa ccacacgtta acattggtac tatcggacac gttgaccatg
     5101 gtaaaactac attaacagct gcaatcacaa ctgtactatc taagaaaaac ggcggccaag
     5161 ctatggctta cgatcaaatc gatggtgctc cagaagaacg cgaacgcgga atcacaatct
     5221 caacagctca cgttgagtat gaaacagata cacgccacta cgctcacgtt gactgcccag
     5281 gacacgcgga ttacgttaaa aacatgatca ctggtgctgc tcaaatggac ggagctatct
     5341 tggtagtttc tgctgctgac ggcccaatgc ctcaaactcg tgaacacatc ctattgtctc
     5401 gtcaagttgg tgttccttac atcgttgtat tcttgaacaa agtagacatg gttgatgacg
     5461 aagaattact agaattagtt gaaatggaag ttcgtgacct attaacagaa tacgaattcc
     5521 ctggtgacga tgttcctgta gttgctggat cagctttgaa agctctagaa ggcgacgctt
     5581 catacgaaga aaaaattctt gaattaatgg ctgcagttga cgaatacatc ccaactccag
     5641 aacgtgacaa cgacaaacca ttcatgatgc cagttgaaga cgtgttctca attactggac
     5701 gtggtactgt tgctacaggt cgtgttgaac gtggacaagt tcgcgttggt gacgaagttg
     5761 aagttgttgg tattgctgaa gaaacttcaa aaacaacagt tactggtgtt gaaatgttcc
     5821 gtaaattgtt agactacgct gaagctggag acaacattgg tgctttacta cgtggtgttg
     5881 cacgtgaaga catccaacgt ggacaagttt tagctaaacc aggtacaatc acacctcata
     5941 caaaattctc tgcagaagta tacgtgttga caaaagaaga aggtggacgt catactccat
     6001 tcttcactaa ctaccgtcca caattctact tccgtacaac tgacgtaaca ggtgttgttg
     6061 aattaccaga aggaactgaa atggttatgc ctggcgacaa cgtaacaatg gaagttgaat
     6121 taatccaccc aatcgctatc gaaaacggta ctaaattctc aatccgtgaa ggcggacgta
     6181 cagttggtgc cggtgtcgtt acagaaatca aagcttaatt cacttaagtt ttaaagtaaa
     6241 gcaacattta ttcggacgta atcgctcgga acgaaggtct taggatcttt gttccgagct
     6301 tttttattta ttgcaggatc aggaaaaaag acaggagtat tataaataga agaaacacga
     6361 aataaaagag tcagtatttt ctaaatgaaa ctttatgaaa acaaaatgtt ttttacaaga
     6421 aaaagccttg ctttgtgagt ttatatatat tataatgaca aaggtgcgtt tactttagtg
     6481 aattgcacag acgtaatcag aatgatttcg tcggctaaga aacgagaggt tgcgacacgc
     6541 ccggacgctt tgccacgaac gagcgtgacg gaaattttcg tggagctatg tctacttttc
     6601 aaaataggcg aaggagggaa caagatggca aaacaaaaaa ttcgtatccg tttaaaagcg
     6661 tatgaacacc gtattttaga tcaatcagcg gataaaatcg tggaaactgc aaaaagaact
     6721 ggagctagcg tgtctggtcc aattccatta ccaactgaac gcagtcttta cacaatcatc
     6781 cgtgcgactc ataaatacaa agattcacgc gaacaattcg aaatgcgtac acacaaacgt
     6841 cttatcgaca ttgtgaaccc aacaccaaaa actgttgatg ctttgatgaa gctagactta
     6901 ccatctggtg taaacatcga aatcaaatta taaaactaaa acaaaaataa tggaggtgta
     6961 ctcatgacca aaggaatctt agggaaaaaa gtgggaatga cacaaatctt cactgaatct
     7021 ggtgaattaa ttccagtaac tgttgtagaa gctactccaa acgtagtact acaagtgaaa
     7081 acaatggaaa cggatggcta cgaagctatt caagttggtt accaagatat gcgtgaagtt
     7141 ttatcaaaca aacctgcgaa aggtcatgtt gcaaaagcaa acacggctcc taagcgcttc
     7201 attcgtgaat tcaaaaatgt tgagctagga gaatatgaag taggaaaaga aattacagta
     7261 gatgttttcc aagcaggaga cattattgat gtaacaggta ctacgaaagg taaaggattc
     7321 caaggggtta tcaaacgcca cggacaaagc cgcggaccaa tggctcacgg ttctcgttac
     7381 catcgtcgtc ctgggtcaat gggtccagtt gcacctaacc gtgtatttaa aaacaaacgt
     7441 cttgctggcc gtatgggtgg caaccgcgta actatccaaa acttggaagt tgtacgtgtc
     7501 gatgcagaaa gaaatgttat cttgatcaaa ggtaatgttc ctggagctaa aaaatcttta
     7561 attactatca aatcagctgt gaaagctaaa taattgtagg aagaaaggag gaactaagga
     7621 atgccgaatg tagcattatt caaacaagat ggaagccaaa acggtgaaat cacacttaac
     7681 gaagaaatct tcggaattga acctaatgaa agtgttgttt acgatgcaat cgtaatgcaa
     7741 cgtgcgtcat taagacaagg aacacacgcg gttaaaaacc gtagcgctgt tcgcggcggc
     7801 ggacgtaaac catggcgtca aaaaggaaca ggtcgtgcac gtcaaggatc tatccgctca
     7861 ccacaatggc gtggaggtgg cgtagtcttt ggaccaacac cacgttctta cagctacaaa
     7921 cttcctaaaa aagttcgtcg tttagcaatg aaatctgtat tgtcagaaaa agttgctgaa
     7981 aacaacttgg tagcaatcga aggcttgaac ttcgacgcac caaaaacaaa agaattcaaa
     8041 caagttcttg ctaacttatc tatcgactct aaagtattgg ttgttctaga aaaaggtaac
     8101 gactttgctg ctttatcagc acgtaactta ccaaacgttt ctgtagtaac ttctgataat
     8161 gtgagtgtgc tagacgttgt atcaaataca aaagtgttag caacacaaac tgctcttact
     8221 caaattgagg aggtgcttgc ataatgaact tactagacgt aatcaaacgc ccagtgatca
     8281 ctgaaaaatc aatgcttgcc atggatgaca agaaatacac ttttgaagtg gacactcgcg
     8341 caaacaaaac tttagtaaaa caagcggtag aagctgcttt tgacgttaaa gtcaaaaacg
     8401 tgaacatcgt gaacgtacgt gcgaaattca aacgtatggg caaatatgca ggatatacaa
     8461 aaaaacgtcg caaagcgatc gttacattga ctgaagattc aaaagaaatc caattattcg
     8521 aagctgctga ataagcacta atagataact cattaaatta ggagggaaaa acgtggcgat
     8581 taaaaagtac aaacctacct caaatggtcg tcgtaacatg acaggttctg attttgctga
     8641 aatcacaaca tcaacacctg aaaaatcatt gttacaacca ttgaaaaacc atgctggacg
     8701 taacaacaac ggccgcatca ctgtacgtca tcaaggtggc ggacacaaac gccaatatcg
     8761 cgtgatcgac ttcaaacgta acaaagataa cgttgttgca actgttcaaa cgatcgaata
     8821 tgatccaaac cgttcagcta acatcgcgtt agtacactat gaagatggag tgaaagctta
     8881 catcttagca cctaaaggac tagaagtggg tatgaaactt gtttcaggtc cagaagcaga
     8941 tatcaaagtc ggaaatgctc ttccattaga aaacattcca gttggtactg ttatccacaa
     9001 cattgaaatg aaacctggta aaggtggaca attgatccgt tctgctggta caagtgctca
     9061 agtacttggt aaagaaggca aatacgtatt gatccgcttg aactctggtg aagttcgtat
     9121 gatcttagct gcatgccgtg ctacaatcgg ttctgtaggt aacgaacaac acgaattaat
     9181 caacatcggt aaagctggac gctctcgttg gatgcgtaaa cgcccaactg tacgtggtag
     9241 cgtaatgaac cctaatgatc acccacacgg tggtggtgaa ggtaaagctc ctatcggacg
     9301 taaagcacca gtttctcctt ggggtcaacc agctcttggt tacaaaactc gtaacaaaaa
     9361 agctaaatca gacaaactta ttgttcgtcg tcgcaaaaat aaataattac tttgaccatg
     9421 tgtcgcacat tttgagagga ggttcaccat gggtcgtagt ttaaagaaag gaccttttgt
     9481 cgatgagcat ttaatgaaaa aagttgaagc tcaagcaggg gttgaaaaga aaaaagttat
     9541 caaaacttgg tcacgccgtt caacagtttt cccaagtttt gttggatata ccatcgcagt
     9601 ttatgatgga cgtaaacatg taccagttta catccaagaa gacatggtag gacacaaatt
     9661 aggtgaattt gcacctacaa gaacttatcg tggacatgct gctgacgata agaaaacgaa
     9721 acgctaattt gaggggagga taaatccaaa tggcagaaca aatcacatca gctaaagcaa
     9781 ctgcaaaaac agttcgcact tcacctcgaa aagcccgttt agtaattgac cttatcagag
     9841 gtaaaagtgt cgcagatgca atttcaatct tgaaattcac accaaacaaa tctgctggaa
     9901 tcattgaaaa agtattgatg tcagcagttg cgaatgcaga aaataacttt gacttagatg
     9961 ttgaaaactt ggtagtaagc gaagcttttg ttaacgaagg acctacaatg aaacgtttcc
    10021 gtcctcgtgc aaaaggatca gcttctccaa tcaacaaacg tacaagtcat atcacggtag
    10081 tcgtatcaga aaaataagga gggataatca gtgggtcaaa aagtacatcc aattggaatg
    10141 cgtgtaggca tcatccgcga ctgggatgct aaatggtatg ctgaaaaaga gtatgctgag
    10201 ttcttacacg aagatttaag aatccgtaaa tttatttcaa ctaaacttgc tgatgctgct
    10261 gtatcaacag ttgaaatcga acgcgcaaca ggtcgcgtaa acatttcaat tcacacagct
    10321 aaaccaggta tggttattgg taaaggcggt tctgaagtcg aaagcctaag aaaagaatta
    10381 aacaaattaa ctggtaaaag aatccatatc aatatcgtgg aaatcaaaaa accagatcta
    10441 gatgcaaaat tagtaggtga aggaatcgca cgtcaattag aaaaccgtgt tgctttccgt
    10501 cgcgctcaaa aacaagccat ccaacgctca atgcgtgctg gcgctcaagg gatcaaaact
    10561 caagtatctg gtcgtctaaa cggagcagat atcgctcgtt cagaaggata ctcagaagga
    10621 actgttcctc ttcatacttt acgtgcggac attgattacg catgggaaga agcagataca
    10681 acatacggaa aactaggagt taaagtgtgg atctatcgtg gagaaattct tccaactaaa
    10741 aaaaacactg agaaaggagg gaaataatca tgttagtacc taaacgtgta aaacaccgtc
    10801 gtgaattccg cggaaaaatg cgcggggaag ctaaaggcgg aaaagaagta gcattcggtg
    10861 aatacggttt gcaagctgtt gattcacatt ggatcacaaa ccgccaaatc gaagctgctc
    10921 gtatcgcaat gactcgttac atgaaacgtg gtgggaaagt atggattaaa attttccctc
    10981 acaaatctta tactgccaaa gcaattgggg tacgtatggg ttctggtaaa ggggcacctg
    11041 aaggatgggt tgcaccagta aaacgtggta aaatcatgtt tgaaatcgca ggcgttcctg
    11101 aagaagtagc tcgtgaagcg ttacgtctag cttctcacaa attaccaatg aaaactaaga
    11161 tcgtaaaacg tgaggaaatg ggtggtgaat cgaatgaagg ttaaagaaat cagagaatta
    11221 accactgccg aaatgcttga tcaagaaaaa caattaaaag aagaattgtt taatcttaga
    11281 ttccaattag caacaggtca attagaaaac actgcacgta ttaaagaagt acgtaaatcg
    11341 attgcacgca tcaaaactgt gttgcgtgaa caagctaagt aattatggaa ggaggccatt
    11401 aagcgtatga ctgaaggaag aaatcaacgc aaagtttatc aaggtcgcgt ggtttcagac
    11461 aaaatggata aaacaattac cgttgctgtc gaaacaaaga aaaaccaccc tatctatggc
    11521 aaacgcatga actattctaa gaaatacaaa gtgcatgatg aaaacaacac agcaaaagtt
    11581 ggcgacatcg tgagaattat ggaaactcgt ccattatcag ctacaaaacg tttccgtttg
    11641 ttggaagtag tcgaagaagc agttattatc taataacacg agattttcct atatagattg
    11701 aatgcaaaat ctgaaaggag gatacacaac gtgatccaag cagaaagtcg tttaaaagtc
    11761 gctgataact caggcgcacg tgaaattttg acgatcaaag tccttggtgg atctggtcgt
    11821 aaaactgcta acatcggtga tgtgatcgtt gctacggtta aacaagcaac gccaggtggg
    11881 gttgtcaaaa aaggtgacgt cgttaaagcc gttatcgttc gaacaaaatc aggagctcgt
    11941 cgtacagacg gttcatatat taaatttgat gaaaatgctg cagtgattat tcgtgatgat
    12001 aaaagccctc gtggaacacg tatctttggg ccagttgcac gtgaactacg tgaaaacaac
    12061 ttcatgaaga tcgtttcact agcaccagaa gttttataat cttgagacac ttatcaaagg
    12121 aggtgcgaaa cagttatgtt tgttaaaaaa ggcgataaag ttaaagttat cactggaaaa
    12181 gacaaaaaca aagaaggcgt tgtactagca gcgttcccga aaaaagataa agttattgtc
    12241 gaaggtgtaa atgtcgttaa aaaacaccaa aaaccttctc aagctgctcc tcaaggtgga
    12301 attgttgaga tggaagcgcc aattcatgtt tctaacgtga tggtggttga ctctactggt
    12361 gtagctggcc gagttggcta caaagaagta gacggcaaaa aagtccgtgt ttctaaaaaa
    12421 accggtgaag ttttagataa ataaaagttt aaggaaggag ggtcttactg aatgaaccgc
    12481 ctaaaagaaa aatatcttaa agaagtaact ccatcattga tggaaaaatt taactatagc
    12541 tctgttatgc aaacacctaa agttgaaaaa atcgtcatca acatgggtgt tggtgatgcg
    12601 gtatcaaacg ctaaaaactt agataaagca gttgaagaat taactttgat cactggtcaa
    12661 aaaccaatga ttacaaaagc taaaaaatca atcgctggat tccgtttacg tgaaggaatg
    12721 ccaattggtg caaaagttac attgcgcgga gaaagaatgt acgaattctt agacaaattg
    12781 gtttctgttt cactaccacg tgtacgtgac ttccatggtg taagtaaaaa agcctttgac
    12841 ggtcgtggaa actatacttt aggtattaaa gaacaattga tcttcccaga agtagattat
    12901 gatttagtag ataaagtacg tggtatggac atcgttattg taacaacagc aaactctgat
    12961 gaagaatctc gtgagttgtt gacacaatta ggcatgccat tccaaaaata attaaaggag
    13021 gcgaactacg tggctaaaaa atcaatgatt gctaaaaaca aacgtcctgc aaaattttca
    13081 acacaagctt atactcgttg cgaacgttgc ggacgtccac attcagttta tcgtaagttc
    13141 catctttgcc gtatttgctt ccgcgaactt gcctataaag gtcaaattcc cggcgtgaag
    13201 aaagctagct ggtaaaaagt aaattcgcat aagaaggagg taaaagatca atggtcatga
    13261 cagatccaat tgcagatttt ctaactcgta tccgtaacgc aaacatggtt aaacatgagt
    13321 ctttagaagt acctgcatca aaaattaaac gtgacatcgc tgaaatcttg aaacgtgaag
    13381 gtttcgttcg tgatgttgaa tacatcgaag atgacaaaca aggcgtgatc cgtgttttcc
    13441 taaaatttgg taaaaacggc gaacgtgtta tcacaaactt aaaacgtatc tctaaaccag
    13501 gtttgcgtgc ttatgtcaaa gctgacgaag taccaaaagt attgaacggt ttaggtatcg
    13561 caatcatttc gacttctgaa ggtgttgtca ctgataaaga agctcgtgaa aaaaatatcg
    13621 gcggcgaagt tatcgcttac gtatggtaat caagtagaat ctacaaggag gtgtctttaa
    13681 gtgagccgta tcggaaataa agtcgttgtt atccctgaag gagtaacagt tactcaagac
    13741 ggaaacaacg ttacagttaa aggaccaaaa ggtgaattga ctcgtacttt ttcagcagat
    13801 atcaaaatga atatcgaagg aaacgaagta actttcactc gtccaaacga cagtaaagaa
    13861 atgaaaacaa ttcatggaac aactcgtgcg aacttcaaca acatggttgt aggtgtcagc
    13921 gaaggtttcc aaaaagcgtt agaactaatc ggggttgggt accgtgcaca attacaagga
    13981 tcaaaacttg tattgaacgt tggttactca catccagtag aaatcacacc accagctgga
    14041 gtaactgttg aagtaccagc taacacacaa gtaattgtta aaggtgcgaa caaagaagaa
    14101 gttggcgaat tagctgcaaa cattcgtgga actcgtcctc cagaacctta taaaggtaaa
    14161 ggtattcgtt atgttggcga atatgtacgc cgtaaagaag gtaaaactgg taaataatcg
    14221 tttagaaaaa ttgcatagaa gccagcagca ggaaaaaggc atttggctga agtttacctt
    14281 caaaaaaata catttttact tgtgccgcag cttctatttt tcgaaacgta tttactgctt
    14341 gaaatagtag tacaataaaa ataaagaggt gacaattgtg attacaaaac cagataagaa
    14401 caaaacacgt caaaagagac atcgtcgtgt acgtaacaat atctctggta ctgctgagcg
    14461 cccacgcttg aacgtttttc gttctaacaa aaacatctac gctcaaatta ttgatgacgt
    14521 agcgggtgta acgctagcaa gtgcctctac cttggataaa gaaatttcag gtggaacaaa
    14581 aacagaagca gcacaagctg tcggtaaact agttgctgaa cgtgcagctg aaaaaggcat
    14641 taaagtagta gtctttgacc gtggtggata cctttaccat ggccgtgtgc aagctttagc
    14701 tgaagctgct cgcgaaaatg gactagaatt ttagaagaag gaggaatacc attcatggtt
    14761 tatatcgatc caaaacattt ggaattagaa gaccgcgttg ttgcaattaa ccgtgtaaca
    14821 aaagttgtta aaggtggacg tcgtctacgt tttgctgctt tagttgttgt cggtgacaaa
    14881 aacggacacg tagggtttgg tacaggtaaa gctcaagaag ttcctgaagc aatccgtaaa
    14941 gcaattgaag atgcgaagaa aaacttagtt gaagtaccta tggttggttc aacaatccca
    15001 cacgaagtaa tcggtgcttt tagtggtggt agaatcctaa tgaaacctgc tgtagaaggt
    15061 tctggggttg ctgctggtgg accagttcgt gccgtattag aattagctgg ggtagcagat
    15121 atcacttcta aatctttagg ctcaaataca ccaatcaacg ttgttcgcgc aacagttgaa
    15181 ggtttgaaac aattgaaacg tgcagaagaa gttgcagaac ttcgcggcaa atcagttgaa
    15241 gaattaatcg gataaggagg acaacaataa tggctgaatt aaaagttact ttaaaacgca
    15301 gtattatcgg acgtcctcaa aaccaacgcg atacagttaa agcgttaggt cttacaaaga
    15361 ttaacagttc agtggttaag ccttctaatg aagcaattaa aggcatgatc aatactgttt
    15421 ctcacctagt ggacgttgaa gaaatttaat ttagaaaaat cataactaga acattaagga
    15481 ggtgccaaac ctatgaaact tcatgaatta aaacctgctg aaggttcacg ccaagtacgt
    15541 aaccgtgttg gtcgtggtac ttcatctggt aacggaaaaa ctgccggacg cggacaaaaa
    15601 ggacaaaaag ctcgttcagg cggcggtgtg cgtttaggat ttgaaggggg acaaacacca
    15661 ttgttccgtc gtttaccaaa acgaggattc acaaacgtga accgtaaaga ctatgcagtc
    15721 attaatcttg atgtattgaa ccgctttgaa gacggagctg aagtaactcc tgttgcttta
    15781 gttgaagctg gaatcgtgaa aaacgaaaaa gctggaatca aagtattagc taatggggaa
    15841 ctaaacaaga aattaactgt gaaagcagca aaattctcta aagcagctca agaagcaatt
    15901 gaagcggctg gtggatcaat cgaggtgatc taatgtttag actattaaaa gatgctttta
    15961 aagtaaaaga cattagatcg aaaatcctat ttactgtatt tgtgttgttt gtatttcgcc
    16021 taggtgcaca tattactgta ccaggcgtaa atgctgcttc attgcaaaac ctttcttctt
    16081 tgccattttt caacatgttg aacttggtaa gtggtagtgc gatgcaaaac ttttccattt
    16141 tctcaatggg ggtttcacca tacattactg cttctatcgt cattcagctt cttcaaatgg
    16201 acatcgtgcc tcgatttgtt gaatggtcga aacaagggga agttggtcgt aaaaaactga
    16261 atcaagcgac tcgatatctg acattagtat tggcatttat ccaatctgtc ggtatcactg
    16321 ctggattcaa tcaatacgct caattaggtt tcgtgaataa tcctaacgta gcaacttacg
    16381 taacgatcgg tttgattcta acagccggta cgatgtttgt tacttggcta ggagaacaga
    16441 tcacagaaaa aggtatcgga aatggcgtat caatgatcat ttttgcaggt atcatttcta
    16501 gattgcctga aagtatcaga gaaatttatg aagattactt tataaatatt gatccttcag
    16561 acatatggaa atctgctatt tttgtggcca ttttactagt agcggtcttg ttgatcgtga
    16621 cattcgtaac atttttccaa caagctgaac gcaagatccc gatccaatat acgaaacgcg
    16681 tagctggcgc gcctacaagc agctatctgc cgttgaaagt aaatgcagct ggagttattc
    16741 cagttatctt tgctagttcg tttattgcta cgccaaatgc tgttttacaa gcattgagcg
    16801 gtagattcgg ttctgaaaac tggtacaaag tgatgatgga gatctttaac tataatacag
    16861 cacctggtgc tacgatttat acgattctga tcgttgcctt cactttcttc tacgcctttg
    16921 ttcaagtaaa ccctgagaaa ttagcggaaa acttgcaaaa gcaaggaagt tatataccaa
    16981 gcgtacgccc tggtaagggg actgaagagt atgtctcaag attactgatg cgtttaagta
    17041 cagttggtgc agtcttcctt ggtttagtgg cattgcttcc aatcattgct caaatggttt
    17101 gggatcttcc acaatcaatc ggattaggcg gtacaagctt actgatcgtt atcggtgtag
    17161 ctttagaaac tgcgaaacag ttagaaggct taatgctgaa acgtaaatat gttggattca
    17221 ttgagtaaga aagcgtgttg aggtttcccc tcaacacacg ttctcacaca tttattagga
    17281 gggaaaacaa tgaacctcat tttaatgggg ctgccaggcg caggtaaagg aacacaagct
    17341 gaaaaaatca ttgatgctta tggtattccg cacatctcaa caggagatat gttccgcgca
    17401 gcgatgcaaa acgaaacagc tcttggcctg gaagcaaagt cttatatgga taaaggtgca
    17461 ttagttcctg atgaagtaac aaatggaatc gtgaaagagc gactagctga accagataca
    17521 gagaaaggct tcttattaga tggctttcca cgtacgctag atcaagcaaa agctctggac
    17581 gcaatgctaa aagatttgaa taaaaaaatt gatgctgtca tcgatatcca tgttggtgaa
    17641 gaaatcttgg tcgaacgtct agccggccga tttatctgca gcaattgtgg agcaacttac
    17701 cataaaattt tcaaccctac aaaagttgaa gacacatgtg atcgttgcgg tgggcatgaa
    17761 ttctatcaaa gagaagatga taaacctgag acggttaaaa atcgtctggc tatcaacatc
    17821 aaaaatagtg aaccaatctt ggcttattat aaagaacaag gtttgctaaa cactattgat
    17881 ggtgctcgtg aaatcgatgc tgtatttgca gatgtacaaa aaatcattga aaaatagcac
    17941 gattgcccgt agccgacttg cattatgcag ttactcttgt caagttcaat aaattatgat
    18001 agaatatgtc agtccgactt ctagagaatc ctctagatga tccattttga gaattttgag
    18061 gtgggaactg cagtttccgc cattttatcg tgtgaaaatg cgaaacaagt acaaggaggt
    18121 actgtgcgtg gcaaaagaag atatgattga agtcgaaggt acagtcgtcg aaactttgcc
    18181 gaatgcaatg tttaaagttg aactagaaaa cggacaccaa gttcttgcta ctgtttcagg
    18241 taaaattcgt atgcattaca tccgtatctt acctggtgac aaagtaacgg ttgaattgtc
    18301 tccatacgat cttaaccgtg gccgtattac ttatcgcttt aaataattgt actccgtaaa
    18361 actacaggag gtattatcat gaaagtaaga ccatcagtaa aaccaatgtg cgaacattgt
    18421 aaagtaattc gtcgtaaagg acgcgttatg gtgatttgcc cagcaaatcc aaaacataaa
    18481 caacgtcaag gataattgga ggtgctataa atatggctcg tattgcagga gtagatattc
    18541 ctcgtgataa acgtgtagta gtttcactta cttatatcta tggtattggt aacactactg
    18601 ctaaaaagat cttagctgat gctggtgtat ctgaagatgt tcgcgttcgt gaattaacga
    18661 atgaacaaac agatgctatc cgtgcagaag tagataaatt aaaagttgaa ggggatcttc
    18721 gtcgtgaagt gaacttgaac atcaaacgtt tgatggaaat cggttcttac cgtggaatcc
    18781 gtcatcgccg tggattgcct gttcgtggac aaaacacgaa aaacaatgca cgtactcgta
    18841 aaggcccagc tcgcgctatc gctggcaaga aaaaataatt cttaagtgaa ggaggttaaa
    18901 acttcatggc agcaaaaaaa gtgaatcgta aacgccgtgt gaagaagaat atagaagcag
    18961 gtattgcaca tatccattct acattcaata acactatcat catgatcact gatactcacg
    19021 gaaatgcatt agcgtggtca tcagctggtt cattaggttt caaaggaagc aaaaaatcaa
    19081 ctccttttgc cgctcaaatg gcagcagaaa ctgctacaaa agcagcaatg gaacacggat
    19141 taaagactgt tgaagtaaca gttaaaggac ctggttctgg acgtgaagca gcaattcgtt
    19201 cattacaagc tacaggttta gaagtgactg caattcgtga cgtgactcca gttcctcata
    19261 atggtgcccg ccctccaaaa cgccgtcgtg tttaatgagt gccgctcatt ttgagtttta
    19321 tcagtaacgt cattgaacaa gacacttttc gttttgaaag gggtaaagat aagaatgatt
    19381 gagttcgaaa aaccaagaat cgcaaaaatt gatgaagaaa aagattatgg caagttcatc
    19441 gttgaacctc ttgaaagagg gtatgggact actttaggga attccctacg tcgtatttta
    19501 ttatcttctt tgccaggtgc tgccatcact aatattcaaa tcgatggtgt actgcatgaa
    19561 ttctctacca tcaaaggtgt tagagaagac gtcactcaaa tcatcttgaa cattaaaggc
    19621 ttagcgctga aaatgtatgg ccaagaagaa aaaacccttg aaatcgatat taccggacct
    19681 gctacagtaa ctgctggcga tattatcgtt gatagtgatg tagaaattct taacaaagat
    19741 atgtacatct gtagtgtctc tgaaggagct acgttccatg ctcgcttaac agtgaaacct
    19801 ggacgtggtt atgttcaagc agatgaaaat aaaaaagaag atatgccaat tggcgttctt
    19861 ccagtcgatt caatctacac acctgttcgt cgtgtgaact atcaagttga gaacacacgt
    19921 gttggacacc gtgaagattt tgataaattg acgatggaga tttggacaga tggttcaatt
    19981 gaaccgttgg aagcaatgag tttgtctgcg aaaatcatga ctgaacattt ggatattttt
    20041 gtgaacttaa ctgatgaagc aaaaaatgct gaaatcatgg tcgaaaaaga agaaacacaa
    20101 aaagaaaaaa tgttggaaat gacgatcgaa gagttagact tgtctgttcg ctcttacaat
    20161 tgtttgaaac gtgcaggaat caatactgta caagaactta caaataaatc tgagcctgaa
    20221 atgatcaaag tccgtaactt aggacgtaaa tcattagaag aagttaaagc aaaattgcac
    20281 gacttaggtt taggtttacg taaagaagat taaaaccttt tacgaaggag ggaaaccaac
    20341 gtgagttatc gtaaactagg acgcacatct agccaacgta aagcgatgtt gcgtgattta
    20401 acaactgatt tattaatcaa cgaacgtatt gtgactactg aagctcgtgc gaaagaagtt
    20461 cgctcaactg cagaaaaaat gatcacttta ggtaaacgcg gagatttgca tgcacgtcgt
    20521 caagctgcgg ctttcgtacg taatgaagtt gccagcgtgc gtgaagaaaa tgaggacatc
    20581 gtcattgaat cagctttaca aaaactgttc aacgatattg cacctcgtta cgcagaacgt
    20641 caaggcggat acacacgcat tttgaagaca gaaccaagac gcggtgatgg tgcaccaatg
    20701 gttgtcttag aacttgtcta aaatcacact aataacatca ctttatagat gattctttta
    20761 gagtgttatg atgttggaag atatttttcc gagtctagct cagcgctgct cctcaagcga
    20821 atatcgcttg gggagtaagc actcatcata aagtgttttt ttataccttc aattacctgt
    20881 catgacagat atgtttgaag gcataaaaaa acattttaag cgagaaataa ttgaaaattt
    20941 tgtctttttt tgatagtgtt agtaggaact ttaggatgaa gaaggagtaa tcatggagcc
    21001 aattattgaa ttagaaaaga taaattataa ataccagcca gatgatcttc gccccgcatt
    21061 gaaagatgtc tcctttacaa ttgataaagg tgagtggatc gctattattg gacacaatgg
    21121 ttcaggaaaa tcgacacttg ccaaaacgat caatggattg cttctgccag aatcagggat
    21181 cgtgaaagtg ggaaatcaaa tattggatga agaaaatatt tggacgatcc gacagatggt
    21241 tggaatgatt tttcaaaatc cagacaatca gtttgttggt tcgacagtag aagatgacgt
    21301 tgcttttggt cttgaaaatc aagggattcc cagagaagaa atgctagtgc gtgtgaaaga
    21361 tgcacttgag aaagttagaa tggctgagtt tgcttcacgc gagcctgctc gtttatcagg
    21421 agggcaaaaa caacgtgttg ccattgccgg cgtggttgcc ttacgacctg atatcattat
    21481 tttagatgaa gcaaccagta tgctagatcc agaaggtcgt gaagaagtga tctctacgat
    21541 caagaagatc aaagaagaaa gtcaattgac cgttatatct atcactcatg atattgatga
    21601 agcagctaat gccaatcgga tactagtgat gagacaaggc gaacttgtcc gtgaaggtac
    21661 accaaaagaa attttttctg ctggtcctga actgatcgac ttaggtctag atctaccatt
    21721 tcctgaaaaa ctgaagagcg cactgaaaga acgtggggtt gatgtgccca gtgaatatat
    21781 gacagaagaa aggatggtgg attggttatg gacatccgtt ttgaacaagt agacttcact
    21841 tatcagccga atacaccatt tgaacaaaga gccttatttg acatcaatat gacgatcaaa
    21901 gaaaacagtt atactgcatt agtagggcat accggaagcg ggaaatcaac cttacttcag
    21961 catttgaatg cgcttgttaa gccaacaagc ggtacagtcc atattggaga acgagatatt
    22021 cagccggata cggataataa gaatttaaag cccatccgaa aaaaagttgg catcgtcttt
    22081 cagtttccag aagcacagct attcgaagaa acggtagcaa aagacattgc tttcggtcct
    22141 aaaaactttg gtgtcagcga agaagaagca ctagtcctag caaaagaaac attagaacaa
    22201 gttgggctgg atgaaagcta tttggaacgt tcgccatttg aactttcagg agggcaaatg
    22261 cgtagagtag caatcgctgg tgtgcttgcc atgaggccgg aagtacttgt attggatgaa
    22321 ccaacggcag gacttgatcc ccagggcaga aaagaaatga tggagatgtt ttggcgtctt
    22381 cataaggaac agcagatcac tattgtatta gttacgcatt taatggatga tgtagccaat
    22441 tttgcagatt atgtctatgt gttggaaaag ggacgaatcg ttaatagcgg ggagccgcaa
    22501 gaggtctttc aaaacattga atggctaaaa gaaaaacaac taggcgtacc tactgccacc
    22561 gagtttgcag aagaattaat ggctaaaggg atgaactttg ctaccttgcc tttgacagct
    22621 gaagaattag cagacgctat cgtccattat gctggaggaa atgcccatga tgaataaatt
    22681 gatatttggt cgttatatgc ctggagattc cttgatccat cgattggatc cccgagctaa
    22741 attattagcc agtttttatt ttatcggaat cattttttta gcaaataatt gggaaacgta
    22801 ccttttgttg gcatttttca cattactttc tgtcttcttg tcaaaagtag atttgaaatt
    22861 ttttatccga ggcattcgcc cattgatctg gctaatcctc ttcactgtag ctttgcaggt
    22921 cttgtttaca agtggcggag aaacttattg gagttggggt gttttccata tcactgaatt
    22981 tggtgtgatg aacggcctct ttattttctg tcgattcgtg ttgatcattt ttatgtcgac
    23041 tctactcacg cttactacac cgcctttgga attatcggat gcgattgagt acttgcttcg
    23101 tccattgaaa gccgtacggt tcccagtcca cgaaatttca ttgatgctgt cgattgctct
    23161 tcgttttgtg cctacattga tggacgaaac agaaaagatc atgaacgctc agcgcgctcg
    23221 cggtgttgat tttggtgaag gaaacctagt tcagaaaatg aaagcagtca ttcctttgct
    23281 gattccttta tttgtcagca gcttcaaccg tgcggaagat cttgcgactg ctatggaagc
    23341 aagaggctac aaaggtgggg aaggaagaac gaaatatcgt gtattgcatt ggcataatag
    23401 agacacgatg gttatcattg cttttgtatt attaactgtg ctactgatct ttttaagagg
    23461 ataaacgttc agtgggccgc tatctattct tttcttgctg atctgtttgc aagaaaggat
    23521 gcaagcagtg tatgacaaga aatattaggg tctgtggcag actaaggtca caggcctctt
    23581 tcttttatct gaaaagaaag aacgaaaaaa ttttatagaa aaatcattat tccagataac
    23641 cttttaggaa caaaggagag aaaaacatgg tacgttacaa agcaatcatt gcgtacgacg
    23701 gaacaaattt taatggattc caaaaacagc caaatgggcg tactgttcaa gaagaagtag
    23761 aaaaaacatt acaaaaaatg gcaaatggaa aagaaatcac ggtctttggt tcgggaagga
    23821 cagatgccgg agtacatgcg atcgggcaag taatccattt tgattatcca gaggagcgtc
    23881 cgttagaacg aatgcgtttc gcgctagata cccagtcacc agaggatatt gctgtaagac
    23941 aagtagaaat tgtttcagaa gacttccacg caagatattt ggtcaaagaa aagacgtatc
    24001 aatttcgagt ggatataggg aaaccacgca gtccttttcg acgccattat gcaagttatt
    24061 ttccttaccc acttgatttg gaaaaaattc agcgggcgct tcctgattta ttagggactc
    24121 acgatttcac ctctttttgt gcatcaggaa gctcgataga ggataaaata cgtacgatct
    24181 acgaggctaa gatggaggta aatgaaacaa aagacgaatt gttgtttaca tttaggggaa
    24241 atggcttctt gtataagatg atacggatct tagtaggaac gcttttgaaa attggcaatg
    24301 gccgattgcc tgaagatagt atcccagata ttattgcgaa aaaagatcgt aatgcagcag
    24361 gacctacagc acaccctgaa ggactttatt tgtatgaagt gaaatatgaa taagaggtta
    24421 tgatagaagt ggtcagcttc aagaaataag aaggtattta cgaaaattac ttctcaaaat
    24481 tttgcaaaat ttcggcttat tttcgaagaa gctacttctg gaacaccgtt taagaaagag
    24541 gtcgtgagcc tgtcatttaa ctctgagtca caaagagcaa tttcaaaaaa cagcttctca
    24601 tctttgatga atatcgtgac ttgtggacga ggcgttgata agtgaatcat ctttttgtta
    24661 cctatttttt ctgtaaccaa aaaacgatgg aacgttgttt tgattgccct tggtgcggac
    24721 agtctgaaca ggttccatcg tttctttatt ttctacgtct tcttctgtgt gtgtttcgtg
    24781 gttgttcttc tattgctgga tcaatcgtgc cataaaaggg gcgattgcgg tcaaggtacc
    24841 agacataaag aataaacaat gcaatacata ggacaaaaag gattgcacct atcgtaaatc
    24901 cttcaggcaa gtaactgaaa gtgacagtat gtttacctgc tggaactttt atcatcaaga
    24961 atgcgtcttt gaaattgctg accttgactg gtttcccatc aattttagct ttccatcctt
    25021 tgtcataagg aatagttgtc accaacatgg aatcttcagc ggtatttaca gtcccagaag
    25081 cttttctgcc agtggcttcc atttcgacac ctttttcttt gatctgattc attgcggctt
    25141 gataggctac aacatctaga ccaacgactt taggctccat gaaacttatt tctttcgtcc
    25201 cataaaaact tgcagtaaat gtcactgttg tctcttgagg ataatagcca agatcatagt
    25261 attgaccaga aatgttgatt tgcgtttttc gcgatgtacc attgacagtg atcgtcgcac
    25321 tagaactttc taattgcgca aaattggttg ggaacaaact taagtaagct tgtgtatttg
    25381 caggcacatg gacttcccaa gtgagctctt ttggctggtt atctgctgtt tctgagtaag
    25441 tgacgccagt cgttgtcttt gtgatcgtcg tattcaactg attcttcaaa gtaggtgtat
    25501 agaatgtaaa atactgttca tttagaccag acaatgtatt gatcaagttt gtctgactgg
    25561 tcaaattatc tgtcactggc tgctcaacat cattaacgga caacggtgca gtaaatgcta
    25621 gtggcaatgc attgacattc tgatacagtg tatagtcgcc gctttgacca gcttggcgga
    25681 agccatattt actgaacgtg ctttcgtttt tagaaatgtt atatttgatt cctgtaaacc
    25741 catccatcag caaagtatta ttgttataac ggatattcaa atttgtccct cttgaacgga
    25801 aacctaattt atccaggtaa gaggaagaat tacgatttct tatagaagaa aacaaactga
    25861 taccactgta accatagttg atcgaatcgt ttgcagatac tggatccaga ttttccaagc
    25921 ggtaaaacga gtcggaatct ttttttgttt catttacaag agctttcaag tcaggataag
    25981 gatcagtata taaactgcgg gaagcataat tccaatcatc aagaatgcca tggatcatac
    26041 tgttcgtatt gaccagtgct tcagcagaca tgatcaataa taacaatata ctcagatgat
    26101 tgaatgaaag ttttttatgc tgataaaatc ccacagcaaa agcataaatc cctaagaaaa
    26161 ccactgtcaa tacaaaggaa gtcatcgtca cataggtgta gcttccttca ggtttgatgc
    26221 cccatgccac cgcaaagaga gcagctacga ccaaaatgat tcctaacaac agcccaccat
    26281 cgtcttgttc tatcttttcc catccttttc ctgcaagcag gagcactaag aaagagaaca
    26341 gataggcata acggaataaa aacatattcg gtgcatgcat accatgccag aacaaattca
    26401 atggaacaag ataaaaactt gcgataagta tggcgaaaag actgccgtac aaaaatttat
    26461 ttttcaacgg tactttccga ctggcaaaat aaaacagaca gaggactaat ggtagcagac
    26521 ctgcgtaaac gaacggaatc gatccgtatt ttgtcgtgtc ataaacacca atcatatttt
    26581 tcatgattag atccagataa gaagtcgctt ctgttttcaa cgtagtgatc tgactcagtg
    26641 cctcgccatt tgaccgaaga tccagcactg ctggcaatac aatgatcatt gaagcgccgc
    26701 ctgccagtaa agaagtgata ccataaggaa caatcgtttt tttatattct gaccagtttg
    26761 tcaatagacg ggcgataaaa tataagaaag aaaatacgcc aatcataaaa cccatataaa
    26821 aactggataa aaacaagagt aaataactga taaataaaag tttcggccgt ttaagatcca
    26881 taacacgatg aatccccaaa atcactaatg gaagataaac aaatgcatcc agccacatga
    26941 tgatctccga atgtgcagtg ataaatgaga tcaatgcgta actgacgcta agtgcaacat
    27001 gcccccagcg aggaatcttg aacgtacgtg caagaaacca aaaagccaat ccggcacagc
    27061 cgaccttaag caaagtcaga acatacagtg catctggcat catctggttt ggaaaaaaga
    27121 acaccagtgg cgtgaagatc ccgcctagat agtaagaaat taaagataaa taattcaatc
    27181 ctagagaagc attccatgta taaaaaatac tttgctttcc aagaagaaca tttcggaagc
    27241 tggcatgaaa attagaaaac tgtgaaaagg catcacttgc caaaatactt ctgctgcttc
    27301 caggatagat tccgatactt agataaacaa gtgctaaaat caaaaacgga atcaggaaac
    27361 tagctgctgc gtagcgtccg ttttctctaa agaatgactt taaatttttt ttc
//
LOCUS       DAZULY010000011        24621 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000011 DAZULY010000000
VERSION     DAZULY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.011"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            79..1779
                     /locus_tag="ACG81A_000330"
     CDS             79..1779
                     /locus_tag="ACG81A_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFS5013728.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            1749..1967
                     /locus_tag="ACG81A_000331"
     CDS             1749..1967
                     /locus_tag="ACG81A_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFS5013729.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            1987..2877
                     /locus_tag="ACG81A_000332"
     CDS             1987..2877
                     /locus_tag="ACG81A_000332"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFS5013730.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            2895..3179
                     /locus_tag="ACG81A_000333"
     CDS             2895..3179
                     /locus_tag="ACG81A_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013731.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            3666..4280
                     /gene="gmk"
                     /locus_tag="ACG81A_000334"
     CDS             3666..4280
                     /gene="gmk"
                     /locus_tag="ACG81A_000334"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS5013732.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            4285..4590
                     /gene="rpoZ"
                     /locus_tag="ACG81A_000335"
     CDS             4285..4590
                     /gene="rpoZ"
                     /locus_tag="ACG81A_000335"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS5013733.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            4843..7251
                     /gene="priA"
                     /locus_tag="ACG81A_000336"
     CDS             4843..7251
                     /gene="priA"
                     /locus_tag="ACG81A_000336"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFS5013734.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            7266..7757
                     /gene="def"
                     /locus_tag="ACG81A_000337"
     CDS             7266..7757
                     /gene="def"
                     /locus_tag="ACG81A_000337"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS5013735.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            7750..8688
                     /gene="fmt"
                     /locus_tag="ACG81A_000338"
     CDS             7750..8688
                     /gene="fmt"
                     /locus_tag="ACG81A_000338"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFS5013736.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            8688..10046
                     /gene="rsmB"
                     /locus_tag="ACG81A_000339"
     CDS             8688..10046
                     /gene="rsmB"
                     /locus_tag="ACG81A_000339"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFS5013737.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            10059..10799
                     /locus_tag="ACG81A_000340"
     CDS             10059..10799
                     /locus_tag="ACG81A_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFS5013738.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            10796..12865
                     /gene="pknB"
                     /locus_tag="ACG81A_000341"
     CDS             10796..12865
                     /gene="pknB"
                     /locus_tag="ACG81A_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFS5013739.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            13030..13929
                     /gene="rsgA"
                     /locus_tag="ACG81A_000342"
     CDS             13030..13929
                     /gene="rsgA"
                     /locus_tag="ACG81A_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFS5013740.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            13942..14592
                     /gene="rpe"
                     /locus_tag="ACG81A_000343"
     CDS             13942..14592
                     /gene="rpe"
                     /locus_tag="ACG81A_000343"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS5013741.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            14593..15231
                     /locus_tag="ACG81A_000344"
     CDS             14593..15231
                     /locus_tag="ACG81A_000344"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFS5013742.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            15386..16240
                     /gene="mreC"
                     /locus_tag="ACG81A_000345"
     CDS             15386..16240
                     /gene="mreC"
                     /locus_tag="ACG81A_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFS5013743.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            16244..16753
                     /gene="mreD"
                     /locus_tag="ACG81A_000346"
     CDS             16244..16753
                     /gene="mreD"
                     /locus_tag="ACG81A_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFS5013744.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            17008..18582
                     /locus_tag="ACG81A_000347"
     CDS             17008..18582
                     /locus_tag="ACG81A_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFS5013745.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(18650..20449)
                     /locus_tag="ACG81A_000348"
     CDS             complement(18650..20449)
                     /locus_tag="ACG81A_000348"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFS5013746.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            20572..21078
                     /locus_tag="ACG81A_000349"
     CDS             20572..21078
                     /locus_tag="ACG81A_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS5013747.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            21157..21879
                     /locus_tag="ACG81A_000350"
     CDS             21157..21879
                     /locus_tag="ACG81A_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5013748.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(21957..23357)
                     /locus_tag="ACG81A_000351"
     CDS             complement(21957..23357)
                     /locus_tag="ACG81A_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFS5013749.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(23527..24501)
                     /locus_tag="ACG81A_000352"
     CDS             complement(23527..24501)
                     /locus_tag="ACG81A_000352"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS5013750.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
BASE COUNT         8016 a         4547 c         5255 g         6803 t
ORIGIN      
        1 tggaatctac aaagtcgaat acgagactca tgaccttttt atgacgtact ggtatacttg
       61 aagaaaaagg agagcgcgat ggagataaaa aaagtttact tttcacagaa aatgtcgctg
      121 tattcgccta tccagcaagc aaaacaaccc cttccatttt ttcaaagtca tcaagaaagt
      181 agagacgctt tttggcgttc tgcaaaaaag caatgtgctt ttaaagcagt atcgaaaaaa
      241 tataccgtct attttactat tgctaaaagt agaggagatg ttatttttaa taatctgttg
      301 attgaaggtg aaacttttga atggaaaaaa ttttttcggt atatggaagc ttgcgctcgt
      361 ttttttatta aagaaaactg ttgtttttta tttcaatctc ctctatcaga agaatggttg
      421 cgtatttttc ataaaaacgg ctatcaagga accacacaat taaataaaaa gctggtctat
      481 catacagcac ttgttttagg tggaggagga gcgcacggtg cttatcagat tggtgtgtgg
      541 caggcgctga aggaacatga tatcaacttt gagattatca caggtacctc ggtaggtgcg
      601 ttgaacggcg cattgatttt acaaggagat atgaaaaaag ctgtgaattt atggaaaaag
      661 ctgtcgacaa gacaagtgct tgcgttacca gaaatggctg cagttgaaga ccttcgtgaa
      721 cgcttctatc gagaaagacg ccaaatgacg aaaacagcac taattgaagg cggggtttct
      781 tctgctccat tggaaaagat ggtcaaagac aatttggatt tgtccttact caaacataat
      841 cccaaaatac gtttatatac ggtgtctact aaactgccag aactagcgga agtagtgact
      901 gatatccaac agacaaaaac agaagaaata ccagattgga tactcgcctc tgcttcattt
      961 tatcccgcta tggcttatcg aaagatcgga aaaaataaat atgcagacgg tgggtatcgc
     1021 aacaaaatac ctattgatat tgcgattaac aaaggagcaa cagaggcatt tgtcgtagat
     1081 gtgcaagggc caggcccagc gaaaaaaatt cgtatgccag acatttttat ccattggaaa
     1141 tgtcaaacat tgtggacact aggcagcttt ctgctttttg atagccaaag gaaccaatta
     1201 aatttacaat taggctatct ggaaatgaaa aaacgattag gctattacta tgggaactgg
     1261 tatacatttc attctgttaa acctgcaaga acatactggc gaggattttt gtcttactta
     1321 acaaaagaaa ttcagttaga attatctttt ttcaaaagca taaaattttg gcaaaaactg
     1381 cgtaatttat ataagaacag ggtcgtacca gagacatgcg gattggcgat gctagaactt
     1441 ttagcaaaaa aacatttttt actgcccaat gaaatctatc aagtagacat gatgattcaa
     1501 atgatctgcc aacaagatat aaaaagtatg ccggatgatt tattggcaca aattggtcaa
     1561 ttaagtactg aagaatggcg aaggtatcga aaataccagc aaaaaataca aaatgagcgc
     1621 acgaaaactg tttattttga ttatctgcta caaggaaaac gcaaggatcg tttgcagcat
     1681 gaattgctta agcaaccagt tgacacatta ctgatcctat atttatatta tctcaaggag
     1741 gaacaaccat ggcacaagaa ttttcatatg aaatcgtaga agaaatcgct atattgtcag
     1801 aaaacaacaa ggggtggcgt aaagaactga acctagtcag ctggaatggt cgcccgccaa
     1861 aattcgatct tcgagattgg gcgccagacc atgaaaaaat ggggaaaggc ctgacattga
     1921 cgaacgaaga gtttgaacaa ctgcaaaaag caatagaaaa tatgtaatga ggaaggaccc
     1981 tgtcgaatga aaagtatgac tggttttggc aaagccatcc gcgaaacaaa agcgtatcag
     2041 ttagatattg aaataaaaag tgtcaaccag cgttttctag atgttcaaat acgaagtcct
     2101 aaaatcctga actttgttga aaacgatttt cgtcagctta ttaaacagca gttaagccgc
     2161 ggtcgaatcg aagtatttgt gaatctagct tatattggca aaaacgaaaa acagatcgtc
     2221 atcaactggg aattgattga ttcacttatc actcaattga cagacggaat tcaacaacga
     2281 tacggtagtc aaaatcaagt gtcaattagt cggatggcgg aacgcttcgt gctggacgaa
     2341 aacttcgtaa cggtagaaga aaaaccggaa gagagcatgg aagaattggc acagttagct
     2401 ctagatacat tgaaaagcgc acttgattct attgaggcga gtcgagaaaa agaaggtcaa
     2461 gctttagctt tagtgttgaa aaagaatact gaagaattca aagaagtact aaaaaaactc
     2521 gatagttttg tagaaatcta tgaaaaggaa tttcaaataa ggtatcaaaa aaagctggag
     2581 gaatttcttg gagcgaaagt cgatgagcaa cggttgttga cggagctagc catcttgttg
     2641 gaacgcggag atattcacga ggaactggat cgtctggcga tccatgtaca aaaactagat
     2701 gaacttatcg aagcacaatg tcctgtaggt cgggaattag atttcctgat ccaagaaatg
     2761 aatcgggaaa tcaacacgat cggttccaaa tcaaatgcaa tcgaaattaa aaatcaagtc
     2821 gtccagtcta agacgatctt agaaaaaatc agagaacaag tacaaaatat tgaatgacga
     2881 aagaggtaga gataatgaaa tattatgacg taacgttcca cgaattaagt ggaaaaactg
     2941 tggttaaacg ggatatccct tctgaaaaaa acggctttga cgtatggaaa gatgcctgtg
     3001 cagactataa tgagaacgaa ctgtttattt tgatcaacga cggtgcttac gtgacgatga
     3061 atcggaaatt tattgttcga attgacacag aagaggtaga agatccgaca gaaaaagcac
     3121 gttctcgaaa agatgagatc atgggtgtag tgaatacttt gtccaatatg ggcttttagc
     3181 aaggatgctg aacaaagaaa tacaataaat aaacagaatt ggatttcttc gcttagcttc
     3241 tttgctctag gaatacattt aaaggttgtg acaaaattcg tgtcacaatc tttttttatt
     3301 ttgaataagt gatgttcaaa aaccattttc tcggtattaa gccaaattta tgcaaaaatg
     3361 tgagaaacta ttttcacaaa atttttcttc atactcgaaa ctaatcggct tcttcacgac
     3421 ctcttataca cggcgttcaa aagcttgcat ctgcgataat aaccccaacc aaacgaaaaa
     3481 catgagaagc tgtttatgtt tggctgttct tattacttga tgcaggacag cttttttacg
     3541 acctcttggt acagggtagc ttttttacca atttctagct ataattaaag ataaccttga
     3601 aagttgcaga aaaaaagtgc aaaataaacg ataagtacaa aaagactagt tgaaaggacg
     3661 tcgtcatgtc agagcgtgga ttattaatcg tgttgtctgg tccatccggt gtaggaaaag
     3721 gcacagtacg aaaagcaatt tttgagagtg aggataatga ttttcagtat tcgatctcaa
     3781 tgactacccg taaaatgcgt gaaggggagg tcgaaggcgt tgattatttc ttccgaagca
     3841 aagaagagtt tgaatcaatg atcgaagctg gagagatgct ggaatacgca gaatatgtcg
     3901 ggaattatta tggaactcct ttgtcatatg tcaacaagac attggatgaa ggaaaagatg
     3961 ttttcttgga gattgaagtc caaggagcaa aacaagtcaa agaaaaagtg cccgatggtg
     4021 tattcatctt tttgactccg ccagatttag cggaattaaa atcacggatc gtaggccgag
     4081 gaacagatgc agatgaagta atcgaaaaac gaatgaaagt tgcaaaagaa gaaatcgaaa
     4141 tgatggctct ttacgactat gctgttgtaa atgatgaagt gccgctagca gttgaacgaa
     4201 tcaaaaacat tattgccagt gaacattttc gagtagatcg cgtaatcggt aaatatatta
     4261 aaatgttaaa ggagatgtag tcttatgatg ttaaaacctt caattgactc actattagat
     4321 cgtgtaaatt caaaatattc tctagtgatt ttagcaagta aacgcgcgca tgaattggat
     4381 gcaagtgcac agcctactct agattctttt gattcagtaa aaagcgtcgg tcaagcttta
     4441 gaagaaatcg atgcaggaaa tgtagtcaat gatccacatc ctgaattaaa acgtgaacgt
     4501 ttgaagatgg aagaagagga acgtcaagca caaaaagagc gtgaacaaca cgaattggaa
     4561 tcacgtatcc gtgaagagca aaaaatgtaa taaagaaaaa tagagcgaac atctaaaaag
     4621 aaaagcaaac ccagctgttt ccttttcaaa cattcgccac ttattgtctg gtagaaaagg
     4681 ctgtgtctga actttttgac acagttcttt tttttcttat ctgctgttaa ttttttgtta
     4741 ggatttggca gaagataaga caaatccatc gatctctagt aaaataggca aggaactttt
     4801 tgtcgcttga taagaaaaga caaaaaaagg aggaaacgaa ccatgacatt aacagctgaa
     4861 gtcattgtgg acgtacctac tatgcagacc gatcagccat tcacttattt ggttccctct
     4921 gaaatggaaa cagctttgca agtcgggatg cgagtagaag tcccttttgg taatggcaat
     4981 cgtcatgtcc aaggatttgt tatggctatc caaaaatcag aagaatcagc taatccatct
     5041 ttgaaggcag ttattcgttt gctagacctt gctccggtgg tcaatgaaga attactttca
     5101 ttagctgatt atatgaaaaa aattacttac gcattcaaaa tcacttgtct acaaacgatg
     5161 cttccgagtg tgatgaaagc agaatatgat aaattgatct atcctttggc agacacacca
     5221 gaagttcagg tattgtttga tcagcgagaa gtgatttcct ggaaggaagc agaagaagaa
     5281 ggcagccttt ctcaattgat tcgctggcgc caagagcaat tagtagatat caaatatgaa
     5341 gtccatacac gaaataaagt aaaaacgatt cgtttggtcc gttctttgtt aactgaaaag
     5401 caaatcgaag aagaatgggc aaaattacgg caaaatgcca agaaacaaaa agaacttctt
     5461 ttgtgtttat ctgaactgag ccaagaagaa ccaattgctt atttcaaaaa taaaggaatc
     5521 agtactgctg tcttgaatca aggaaaagaa aagggctggc tggagtttat agaatcagaa
     5581 cgttatcgcg acccttataa agaccgagta tttgaccaaa cgacggcatt ggaactgaat
     5641 gcagaacaaa aaaatgctgt cgaacaaatc atcacagcag gtcagcagca aaaagatcag
     5701 gtctttttgc tggagggaat cactggcagc gggaaaacag aagtatatct tcaggcaatc
     5761 gctgatgttt tgtcagaaaa taaaacggct attatgttag tgcctgaaat cgcattgact
     5821 ccgcaaatgg tcgagcgatt caaaggacgc ttcggagaat cagttgcggt cttacatagc
     5881 ggactttccc aaggtgaaaa atatgatgaa tggcgcaaga ttgagcggga agaagcgcaa
     5941 gtagtagtag gcgcacgatc tgctattttt gccccgctga aaaatatcgg gctgatcatt
     6001 atcgatgaag aacatgaatc aagttacaaa caagatgaga caccgcgtta tcatgcgaga
     6061 gatctagcaa tttggcgcag taaatatcat cattgcccaa tagttctagg aagcgcgacg
     6121 ccctctttag aatcaagagc acgagctcaa aaaggcgttt atcagttatt gcagttaaac
     6181 caccgagcca aagcagctgc acagcttcca gctattgaga tcgtagatat gcgtgaagag
     6241 tttcagaatc atcggacctc tactttttct gctaatttgc aggaaaaaat acaaaatcga
     6301 ttagacaaaa aagaacaaac tgtcctactg ctcaatcgtc gaggatattc gtcttttgtg
     6361 atgtgtaggg actgtggatt tgttcttcca tgtcctaatt gcgatatctc tcttacactg
     6421 catatggata cacgttcaat gcgctgccat tattgcggac atgaagaacc tattcccaat
     6481 cgctgtccta attgtggcgg caataaaatc cgctactatg gaacaggtac tcaaaaagta
     6541 gaagaggaac tccgagaact ttatccgcaa gctaggattt tgcgtatgga tgtagatacc
     6601 actcgaagaa aaggggcaca cgaacagatt ttacaaaaat tcggtgcaaa agaagcagat
     6661 atattattag ggacacaaat gattgctaaa ggtttggact ttccagaagt gacattagtt
     6721 ggggtgttga atgctgatac ctccctgaat ttaccggatt ttcgttctag tgagcataca
     6781 ttccaattgc ttacccaagt ttcaggacga gcagggcgag cggaaaaagc aggcgaagtc
     6841 gtgatccaaa cattcaatcc tcaacattac gccatagaat tggctaaaaa acaaaattat
     6901 gagcagtttt atcagcagga aatgcatgta cggcatcgtg gaggctatcc tccttattac
     6961 tttactgtga aaataaccgc cagccatcct gaagaacaag tagcggcaaa gaaaatcttt
     7021 cagattgcca atcagctaaa agaagtgtta agccctcaaa gcttgctatt agggccaacg
     7081 cccagcatga ttttgagagt gaaaaaccga tattattatc aactaatcat caaatataaa
     7141 catgaaccga atcttcctca tgttttggat gaaattttga atggaagcca gaaagaacag
     7201 cgacaaggtc tttttgtggc aattgacaac gaaccattaa actttattta agaaagggcc
     7261 aaattatgcg ctatcctata ttgatccacc ctaatgataa attaaaaaga actgctcagc
     7321 caatcgatgt aatcacggac gagacaatcg ctttattaga caatttatat gagacaatga
     7381 ttgcgaatga cggaataggg atcgctgctc ctcaagtagg acaaaacaag agaattgctg
     7441 tgatcgaagt agatgaaggc gagaaatttg aactgattaa cccagaaatc atcgaagcaa
     7501 aaggtgaaag ccttgacgta gaaggttgtc tcagtattcc gcatgtctat ggaacggtca
     7561 agcgagcaga tgaagtgacc gtgcgttatt atgatcgaga cggagaagag atagaagtga
     7621 cggcttttgg ctatttagcc cgtgccttcc agcatgaaat cgatcactta gacggtatct
     7681 tgttcattga gaaaatgata cagcagatcc cggaagaaga acttgaagaa tatatggagg
     7741 aacatcttga tgactaagct agtatttatg ggcacacctg ctttttctgt gccgattctt
     7801 gaagggctga tagaatcagg ttacgagatc caggcagtgg taacacagcc cgaccgtcca
     7861 gttgggcgga aacgagtaat tacgccgaca ccggtaaaaa aagcggctct gaagcacggt
     7921 atccgtgtgc tgcagccaga gaaaatctcc ggttcgccag aaatggaaga aatcattgaa
     7981 ttggtgcctg atgtgatcgt cactgcggca tttggccaat tcttgcctga aaagttattg
     8041 caagtaccaa aattaggggc aatcaatgtc catgcttctt tacttcctaa atatcgggga
     8101 ggtgcgcctg ttcattatgc catcatgaat ggggagaaag aaactggtgt aacgatcatg
     8161 gaaatgatca aaaaaatgga tgcaggcgga atctatgcgc aagaaagcat accaatcact
     8221 aaacaagatg atgtagggac gatgtttgaa aagcttagcc ttttaggaag aaagctattg
     8281 ctagaaacat tgcccaatat tttggatgga caaaaaccag tacctcaaga cgagtcagaa
     8341 gcaacttttt cgccaaacat cacacgggaa caagaagcaa tcgattggca taaaactgca
     8401 gaagaaattg acaaccaagt acgcggaatg cgtccttggc caattgcatt cacgacctat
     8461 gagggcatac gctggaaatt attatcggtt gaagtattgg cagaaactac agacaaagca
     8521 cctggtacga tcatccggaa agacaaaaaa aatctatgga ttgcttgcgg taaacagacc
     8581 gtcttagcca tcaatgaatt acagccagct ggtaaaggaa aacaggccgt acatgatttc
     8641 cttaacggaa gcggtcagca agttcagatt ggtcagcagg tgaaataatg aaaaaaatcc
     8701 ctaaaaaaat gaaaaaatca gtacgatata tggcattact gacattagag cgagtgaaca
     8761 agggtggcgc ctactcgaat cttttgttga atgagatgat caaacaaagc gagctaaatc
     8821 aaaaagatgt gggtttattt acagaattga tctatggtac gattagccat cagcgactat
     8881 tggaatttta cgttgcacca ttgatcgcta aagcaaaaaa agtggatgac tgggtcaaga
     8941 tgcttcttta tctttctgta taccaattag aatttttaga taaagtccct gcacatgcta
     9001 ttttaaacga ggcggtcgaa atcgcaaaag taaaaggaaa tcctgggaca gggaaatttg
     9061 taaatggtgt cttgaggaat tatcagcgtc aaggagcacc agatctgcaa ggcattgctg
     9121 atcctatcga aagactttct gtcaaaatca gcctgcctct atggatgacg aaacgactgg
     9181 tggatcaaat cggatacgaa gaaactgaaa agctcggttt atcgctatat cagcctagcc
     9241 atgctagtgc ccgagtagat acacgccgac tttccagaga tcaggccatc actgttttgc
     9301 aagaagaaga gatcacagct gaaaaaagcc aagtcagtcc atacggtata gtagcgaaaa
     9361 aagggcattt agcttctagc tcattgttcc acgatggcgt catgacgatc caagatgaaa
     9421 gttcgatgct tgtagcgccg tctatgcaaa tcgagcctga acatcatgtc ttagatgcct
     9481 gtgcagcacc cggaggtaag acgacgcata ttgcttcttt tttagatccg tcatgcggtg
     9541 gagaagtggt cgctttagat gtacacgaac acaaagtcaa actcattgaa gataatgcag
     9601 cacgtctagg tgtctcttct gctgtctacg ctcaaaagat ggatgctaga gaagtcagta
     9661 aacagttcga agcagagcag tttgatcgga ttttagtaga cgcgccttgt tcaggtcttg
     9721 ggctgcttcg gcgaaaacca gatattcgtt ataaaaaaca gccggatgaa ttagcgaatt
     9781 taccaaaaat ccaattggaa attttagaaa gctgcgcacc aactttaaag tcttccggta
     9841 ttctgactta tagcacttgt acgatcttaa aagaagaaaa ccaagacgtt gtttctgctt
     9901 tcttggaaag acaccctgat tttgagagga ttgatgtttt gaccaatcct atgctacaat
     9961 catcaatcga agagaaaatg ttgactttgt atccgcatca gttttacaca gatggatttt
    10021 tcatttgttg tttgcgcaaa aagtaatgag gtgaaaaaat gcagattgaa tatcaatcag
    10081 atgtaggaag acgacgtaac acaaatcaag actatgccag tgtatttacc agccgttcag
    10141 gtctcaagct tgctgtatta gcagacggga tgggtggtca tcgtgccgga gacattgcca
    10201 gtcagatggc ggtaacgaat ctaggcgaag cttgggaaaa acaagaagct gttgacgatg
    10261 aaaaaatcgc ccaatggttc attcaatcga ttcaagaaga aaatatgctg atttatcagc
    10321 gtggacaaga acagccggaa tataacggta tgggaacaac aatcgtagcg gccgctctgt
    10381 cagatgactg gttcacgatt gccaatgttg gggatagcag agcttatttg attcgtgaag
    10441 ataaaatcat gcaattgacg gaggatcatt ctttagtcaa tgaactagtc aaatcaggag
    10501 aaatcagcga agaaatggca gtgaaccatc caaggaaaaa tattttaaca cgttctgtcg
    10561 gcatgcctgg aacagtcgaa gtagatgttt ctactcatct ttggcaacta aaagatcgac
    10621 tgctgttatg ttcagatggc ttgacgaaca tgctgtctga agaagaaata ggaacgatca
    10681 tcaatcaaga aggcccattg tctcataaag taactgagct gattgatcgt gccaatgaag
    10741 caggcggagc ggataatatc accgttcttc ttatcgaata taaggaggac agtgtatgat
    10801 cgagttgggc aaaaaattaa atggccgtta tcaaatcact ggaaatatcg gtagcggtgg
    10861 tatggcaaat gtatttttgg cgcacgattt aatcttggat cgtgatgtag cagttaaagt
    10921 cttacgattt gactttcaaa atgaccagac tgcgattcga cgatttcaga gagaagcttt
    10981 agcagcaaca gaactcgttc atccaaacat tgttagtgtt tacgatgttg gtgaagaaga
    11041 caacatgcag tatctggtga tggaatacgt caaaggaatg gacttgaaac gatatatcca
    11101 aacacattat cctgttcctt atgaaacagc agtaaatatt atgcagcaga tcctttctgc
    11161 tatttcttta gctcataacc atcaaatcat ccaccgagat ttgaaacccc aaaacgtact
    11221 gatcgataat gaaggtgttg taaaaatcac ggatttcggg attgctatcg ctttatctga
    11281 aacatcgatc acgcagacga atacgatgct aggatctgtt cattatctgt cccctgagca
    11341 ggccagagga agtatggcaa cgaagcagtc agatatttat gctttaggga tcatccttta
    11401 tgagatgttg acgggtagcg taccgtttga tggtgaatct gctgtaacaa ttgctttaaa
    11461 acattttcaa gatgatcttc catctataaa agcacttgat ccaaatgtgc cacaagcact
    11521 ggaaaacgtg attttacggg caacggctaa agaacctgca gaccgttata agtcagccga
    11581 ggaaatgtct gatgatcttt ctacagtatt atcaccagct cgtgcaaacg aagaaaaatg
    11641 gcagcctcat gtgatggata atgaaacaaa agttatcaca ccccttacgg aagatgcacc
    11701 aatgcctgat tcattcaaat ctatgcctct gccaaaagac aagccagaaa acgaaacctt
    11761 accagaggag gaagaagttc tgcctgatcc taaaaagaaa ggacagaaaa aatggtggct
    11821 gatcctatta gctattcttg ctgttttagg aatcggaaca gctgtattct ttgcttctgg
    11881 cgggcgagga gaagttgttg ttcctgatgt ttctgatctt tctgaagctt ctgctagaga
    11941 aacactcgca aaagcgaaat tagaagtagc aacaaaaaca gaagaaattg cagatgacaa
    12001 aatcgaagaa ggtaatgtag tgaagacaga cccggcagcg ggaacgacag tcaagcaaaa
    12061 acgcgaagtc actctgtata ttagtagcgg cacgaaaaaa atcaaattag atgattacag
    12121 tggtaaaagt tatgaagatg ccgaagcaag tttgaaagaa ttagggtttt ctgctgactt
    12181 gattcaaaaa gaagaggaat tcagttcaga ggtagaaagc ggaatgatca tttcgcaatc
    12241 tccatcagaa gatacagaag tcgatcctaa aacagacacg attactttca cagtcagcaa
    12301 aggaccagaa acagtgactt tgagtagtca tgtaggagaa aactacgaaa ctgttcgtaa
    12361 ttggcttgtt acacaaggat tcaatatcaa taatattagt gctacttatg actatagcga
    12421 cactgtacca tcaggttcga tcatcagtca ggatccagcc tcgggtgaag ttgtagcaga
    12481 agaaacttac atcaattttg tcgtaagcca aggaacagag cctaaattga atgatatctc
    12541 cggctataca aaaagcgaag cacaaagtta tttagctagt gtcggtgcag aatatatcgg
    12601 gcatgagact tccgaatatt caaacacggt ggataaagat aaagtgattc gtacagtacc
    12661 aggggcagga acgacaatta ccaaaggaat ggttgtgaac gtaatttact ctaaaggacc
    12721 agatccagcg tcttctagtt ctgaaaaaga ctcttcagac agttctagca gctcaagtag
    12781 ctccagtagc acaagcaact cttcaaaaga agaatcttcc agctccacaa gttcatcagc
    12841 tagcacctca gaaagttcaa aataagtgct gaataaaata tatcagaagg gaatgcgaca
    12901 ttgatttgtt gcattctctt ttttgtgttt ctatctcttt tgatcaattt tcaagccata
    12961 tactttgaga agtttgcatg gaataacacc tactttcctc ttcgtatggt aaaataaaca
    13021 aaaacggagg tggcacttat caaaggacaa atcagaaaag caatcagcgg tttttactat
    13081 gtatacgcaa acggagagac ctatcaaaca agaggaagag gaaatttccg gaatcggaaa
    13141 atcacgccgc ttgtcggaga tcaggtgatc tttgagagtg aaaatcaaac agacggctat
    13201 cttctggaaa tcttgccgag aaaaaatcaa ttagttcgcc caccagtcac aaatgttgat
    13261 cttggaatca ttgtcacaag tatcgtagag cctgcatttt cttataatct acttgaccgc
    13321 tttctagtta ccttggaata cgagtcgatc gaaccgatta tttttttgac aaagaccgac
    13381 ttgtcagacg acccagaaaa aatcgaagag atcaagcaaa cctatactaa aataggctat
    13441 ccggtgattg tacccaatca tacgggtgat acgacagaac tgatccgtta cttcccagaa
    13501 cggctatcgg tctttatggg tcagtctgga gcaggaaaat cgacgctact gaatcaaata
    13561 tcaccggaac tgaatttagc aacaggagag atttctgatg cacttggaag aggacggcat
    13621 acaacacgtc atgtggaact gattcctatc tatggcgggt tagtagcaga tacaccagga
    13681 tttagctcga ttgacttttt gacaattgaa gcaggagaac tgccgagaca atttcctgaa
    13741 tttgtagaag cttctgtcca ttgcaaattt agagagtgca tgcatgctaa agaacctggc
    13801 tgcgaagtaa aacatagagt agaaacaggt ggtatcgcac agacacgtta cgacaattat
    13861 ttgcaattct tacaagaaat cgaaaaaaga aaaccaatgt atgcaaaaaa agaaggtaaa
    13921 cgaaaatagg aggaaacaag aatgaagata gcaccatcca ttttgagtgc agattttgcc
    13981 aatttacaaa gagacattga gttagtggaa aaaggaggcg cagattacat ccacgtagat
    14041 gtaatggatg gccgatttgt cccaaatatc acattagggc caaatattgt tcaggcaatc
    14101 cgtcctgtta ctaaattacc gttagacgtc catctgatga tcgttgatcc agaaaactac
    14161 attccagcat ttgcaaaagc tggagcagat atcattactg tccacgtcga agcaactcct
    14221 catattcatc gggcacttca aatgacaaaa agcttaggag ttaaagctgg agtagttatc
    14281 aatccaggaa caccagtctc catgatcaaa catgttttgc ctttggctga tcaagtacta
    14341 gtcatgactg tcaatcctgg gttcggcgga caaagcttta ttgaagaaac agtagaaaaa
    14401 attacggaac tcgctgatct acgtagagag aacaattgga cttatgcaat cgaagtagat
    14461 ggcggaatcg ttccagaaac agcaaaaatc tgtaaagatg caggggcgga tgtcttcgtt
    14521 gcaggatcat atatctatgg agccgaaaat ccagtcggac aaatcgaacg attaaaagag
    14581 gcattagact aaatgaaaat tttattagct gctggaggta atccgcaaaa ctggccgcag
    14641 tctctgctgt ccaaatacga tgcctatgtc gggatagacc gtggtgcgct gtacctgctt
    14701 caagcaggct acccagtaga tctggcagtg ggtgattttg attcattgtc gaacgaagag
    14761 cagaaggaag tatttgcaaa agccaaagag tatacccgtt cccctgcaga aaaagacgat
    14821 acagatacac agttaggtat cttaaaaaca tttgaacgtt acccgcaagc ggaagtcact
    14881 ttgattggag ctacgggcgg aagattggat catcttttag ccaatctctg gttaggtatg
    14941 gaacctcgct tcaaaccctt tgcccatcag cttattatca gagattgtca aaattatttg
    15001 tcttattatt tgcctggaga gcatatagta agacagatac cagatatgaa gtatttagct
    15061 tattgctgct tgactcccgt ctcgaatctt acattaaaag gcagcaagta tgtattggaa
    15121 aataaagaag tagaatatcc gacatcttat gcaagtaacg aatttctttc agataccgca
    15181 gaattttctt tttcagatgg tatgattgca gttattcaaa gcaaagattg atccataaac
    15241 aaataaaaaa atttgtcttt caggctaaaa atgacagaat ctattatttg ctaaaaaaaa
    15301 gcttaagagg agcaacttac cttttgtttc acggcattta gtgataaaat tgaacacaag
    15361 agtctaatta attcgaggtg tctttgtgaa gaagttcaat cctaataaaa atattattat
    15421 taccttggtt attgttatca tcgtggtgac gatactaagt gtgaccgctg cgcagcgagc
    15481 gaatgaagga aagcccaatt tcttccaatc catcgttaat gacagtgttg caatggttga
    15541 taaaacaatc tctgccccag tacggtgggt acgaaatgga gtagattctg tccataatct
    15601 tttttcaact tatgccgaaa atgagcggct aaaagaaaaa atcgatagct atgatgacgt
    15661 ttcacagcaa aacaagaatt acgagagaga aatcgaagcg ttgaaaaaag aacttgagtt
    15721 gaatcagacg ctgactagtt acgaaaaagt aacggcaaat gtcattactc gttcgcctga
    15781 cagttggcag gacatgctga tcgtagacaa gggttctaaa gacggtatcg aagcaaatat
    15841 ggcagtcatg tcgcaaaaag gattgattgg ccgagtgatc gaggtcaata cggcttcgtc
    15901 taaaatcgaa ttactgtcat cctctaatga aagctccaat cattttccag tacgggtatc
    15961 ttcggctaat ggcgaagcgt ttggtttgct taaaaactat gatgaaaagc tccatgcctt
    16021 agtggtgacc caattaactg gtgatacgga tatcaaagaa ggggatgttg tccagacatc
    16081 cggtcttgga gggaattctc cagctaactt gccgatcggt acggttatta aaacgaaacc
    16141 agatagttat gggctggatc gggaagttta tgtgaaacct tatgcagaaa tgtatgacgt
    16201 gtcagttgtg acgattgtcc aacggctagc ggaggattag tccatgttga agaaagaaac
    16261 aatgaagtac tatctgccaa tcgttttgtt ctttttgatg ttgatagatg gtcatttaac
    16321 aagaatgcta ggggagtggt cgaaaggcac ctatatgtca aatgcccact ttctgatatt
    16381 ggcattatta tgttgcagta tggcgtttga aaaacgttat ttactgatta ccacgattgt
    16441 tctcggggct atctatgacg cttactatat tggcgttatc ggtatctatg cagtagctct
    16501 ccctttaatt gtatggttga tgtatgtaat gaaagacgtt atccatgtca acatctttac
    16561 tgaatttttc agtatgatca tctttgtcac gggttatgaa ttgtttacga tggtggtcca
    16621 gttgattttt aaattagcag tagtaaataa cacgtatttt attacaaggt ttttaggacc
    16681 tacactgctg ttgaacatga ttatatttgt attattcatt tttcccttta agaaattatt
    16741 cagtgatgaa taatttcttt ttttattttt aggtcgaaag cgaaaaagaa tcgttgttat
    16801 cttaagaaat gctagactta acgcggcttt ttcatgatca ttttctgaaa cgaacagttt
    16861 tgtcattttt aaaagttgtt ttgaaacatt tttgtaacat aactattata tcgttgacac
    16921 actgctatgg tacaattttt atatcgttaa gaaagactta aatatattta gatatttatt
    16981 aaattaagcc agtcggagga atgaagagtg aaaaagagtt taatatcagc agtaatggta
    17041 tgttcaatga ccttgactgc cgtagcatca ccgatcgctg ccgcggcgga cgattttgat
    17101 tctcagatac aacagcaaga tcaaaagatt gcagacttga aaaatcaaca agcagatgca
    17161 caatcacaaa tcgatgcctt agaaagccaa gtttctgaaa tcaacacaca agcacaagac
    17221 ctattagcaa aacaagatac attgcgtcaa gaatctgcac aattagtaaa agacattgca
    17281 gatttacaag aacgtattga aaaacgtgaa gatacaatcc aaaaacaagc acgcgaagca
    17341 caagtaagca acacaagctc aaactacatc gatgctgtat tgaatgctga ttcattagca
    17401 gatgctattg gacgcgttca agcaatgaca acgatggtaa aagcaaacaa cgacttgatg
    17461 gaacaacaaa aacaagataa aaaagctgta gaagacaaaa aagctgaaaa cgatgctaaa
    17521 ctaaaagaat tagctgaaaa ccaagctgct ttggaatctc aaaaaggcga tcttttatct
    17581 aaacaagctg atttgaacgt actgaaaact tcattagctg ctgaacaagc aactgctgaa
    17641 gacaagaaag ctgacttgaa ccgtcaaaaa gcagaagctg aagcagagca agctcgtatt
    17701 cgtgaacaac aacgtctagc agaacaagct cgtcaacaag ctgcacaaga aaaagctgaa
    17761 aaagaagcac gcgaacaagc agaggctgaa gcacaagcta ctcaagcttc atctactgca
    17821 caaagctcag caacagaaga atcatcagct acacaatctt caatgactga agaatcatca
    17881 tcagctacac aatcttcagc aacagaagaa tcaacaacac ctgaatcttc aacagaagaa
    17941 tcaacagcgc ctgaatcttc agcaacagaa gaatcaacaa cagcgcctga atcttcagca
    18001 acagaagaat caacaacagt gcctgaatct tcagcaacag aagaatcaac aacagtgcct
    18061 gaatcttcaa caacagaaga atcaacaaca cctgcgccta caacaccatc aacagatcaa
    18121 agtgttgata caggaaacgg cacaggaagt agtactccgg ctccaacgcc aacaccaaca
    18181 cctgaacaac caaaacctgt aacacctgct ccagctcctt ctggaagtgt gaatggtgca
    18241 gcaatcgtag cagaagctta caaatatatt ggtactccat atgtttgggg cggtaaagat
    18301 ccaagtggct ttgactgctc agggttcaca cgctatgttt acatgcaagt aactggtcgt
    18361 gacatcggtg gttggacagt tcctcaagaa tcagctggaa caaaaatttc agtttctcaa
    18421 gcaaaagctg gagacttgtt attctggggg tcacaaggcg gaacttacca cgtagcgatt
    18481 gctttaggcg gaggacaata tatccatgct cctcaaccag gcgaaagcgt aaaagttggt
    18541 tcagttcaat ggtttgcccc tgactttgct gtcagcatgt aagaatgatt aatcaaaaag
    18601 agttagccca ttctggacta actctttttt tattgtgata aaatagaaat cagataaatt
    18661 cttctgtcaa ttccatgaac gtttggatat cttcagtcac catgtcgata cctgcttgcc
    18721 aaaaatctgg ttgggtaaga tcgacattca aatgtttctt cgcaagatct tccgtcgtca
    18781 ttgaggcagt atcgcgtagt aaatggatat attcttcttc aaaatttgct cccttttggt
    18841 tggcgtaagc atagatgccc aaactaaaga ggtagccaaa tgtgtatggg aagttataaa
    18901 acggtacgtc atcaataaag aaatgcaatt ttgcagccca gaaatatgga tggtaagtag
    18961 ctaaagctcc ttgatagcct tctttttgcg cctcaaccat catctctgtg atcttatctt
    19021 ctgagactaa tccttcttgc cgtgcttcat agaatcgatt ttcaaaaatg aatcgggaat
    19081 ggatattcat gaacatagct aaagcatttt gcagttttgt atctagcaaa ttgatttttt
    19141 cttcttttgt ttttgcgact tttaatgtag catcggctac gatcagttct gcgaatgtac
    19201 ttgctgtttc tgcgacattc atggcatatt cgcgatttaa agaaggtaaa tcccacatcg
    19261 tgctgctatg gaaagcatgt cctagttcat gagcaagtgt cgcaacctca ttaacagaat
    19321 tactgtaggt cataaagatc cttgattctt ttgtttctgg taactcagta caataaccac
    19381 caggacgttt cccaggacga tcttctgctt cgatccagct cttttcaaaa gcagattggg
    19441 caaatttagc catttttgga ctgaatttgt taaagttttc gataataaat gctgctgctt
    19501 cgtcaaaact aaaggttttt tctttcaaat cgcctaaaat aataggtgca tcttgatctt
    19561 gccattccat tttttcttta ccgaaaagat tagcttttct agtcaagtaa ttaacaaggg
    19621 gttgtttgtt tttctggatt gtatcccaca tgacagacaa tgtttctttc ttcaaccgat
    19681 tgtactctaa tggtttttgt agaaaatctg tgacaccatg aagttcatag tcagaaagtc
    19741 taaaaccatc taaatgattc aaagtatccg tgaagagggg tgccttttct ttccaggctt
    19801 cttcccatgc tgcgaaaagt gtttctctga cttttggatc tggatcgccc atcatcttat
    19861 tgaaggcttg tccagcagat aattcgacta cttgtccatc ttgttcgaag ggaatcgaaa
    19921 tgctggctac aatcgtatca taatgactgc tccaagcatt tagaccatct aaagaaagcg
    19981 tatttataat gttttcttca gcttctgata ataattgtga gccatcacga cgaatctcgt
    20041 ttaaacgaaa agcaattgtt tcaaacgaag attgagaaag catctgattc caatcattgt
    20101 cagatatttc tgcaaatttc tttgacaata cagtttcggc tgattgcaga cgaggcagca
    20161 ttgcgtaaag cttgcctgat aagacctttg catctgaatc attgacattg gcagaaagca
    20221 atgcagtaat atagctgctg cattgggtaa aaccattcgt aatcgtttct tgcaattgaa
    20281 gaatagcgtt taactgctct gcattgtcag aactaggtga ccattttgtt acgcggtgat
    20341 aatactcgtt catttggtct tccaattgct tcatccgttg gttcaaagca tcagaatgac
    20401 tgcctccgga aaaaatagaa tctagattcc aatttaatga ataactcatg aaaaaactcc
    20461 cttcatattg taatacaaaa ttaagtatag cactttttta tggaagaagt ctctatgaaa
    20521 atgattgttg tttcttcaaa aaaagtctaa gataaaatac agaggtgact tatgaattct
    20581 tcaaaaaaac aaatgaaaaa cggaaatatt tatttagtaa tcgcactgat agtcgttata
    20641 atcttatttt acagttcttc tcaaacttac ggacagcaat cacaagtttc gcgattagaa
    20701 agctggctgc ctaatcagcc atttaaagac acattagcgg gtatacgttt taattatggt
    20761 ggaagtatgg tgagtatcaa ccatctgggc tacgccaaat ttttagagtt ctttttaagg
    20821 aagggcgcac atttcggtac ctattttatt ttgggaggca gcttgttttt aggtgttttc
    20881 cccaagctaa aaatttggtg gctgacagcg atccttgctt ggctttcagc aacagggtac
    20941 gcaggtttgg acgaattcca tcagatgatg accggtggac gaacacctat gttccaagat
    21001 gtcatgttgg attcgatcgg tgctctgact gctgtgatat tatgtgtgct ttttgcggca
    21061 ttcaagaagc gtttatagat tggctgtggc tttgtttttt tgtgaaatag tactagaata
    21121 gaagtgtatt ctcaaaagaa caagggagga aaaatcatgt caggacatag taaatggcat
    21181 aatatccaag gaagaaaaaa tgcccaagat gcaaaacgag ggaaagtctt tcagaaactt
    21241 tctcgtgaaa tttatatggc agctaaagca ggcggtcctg atccatctat gaatccagct
    21301 ttgcggcttg ttgtggacaa agcaaaagct gcaaatatgc ctaacgacaa tgtggaacgg
    21361 gcgattaaaa aggctacgag tgccgtagat gaaacgaatt acgatgaaat cacgtatgaa
    21421 ggatatggtc cgggtggagt aggaatctta gtccatgcat tgacagataa ccgtaatcgg
    21481 acagcgacaa atgtccgagt agcttttact cgaaacggtg gatcactggg agaaacgggt
    21541 tcagtcagtt acttgtttga ccgcaaagga tatatcgcta ttgaacgcga tgggttgact
    21601 gttgatgaag agacgatgtt tgaggatgta ttagaggcgg gggcagaaga tctgcaaact
    21661 tctcctgaag tattcgagat ttatactgca ccagaagatt ttgctgctgt acgagatgag
    21721 ttggaaaaag agttcacttt ggcgcaagca gaattgacga tgattccaca aacgacgatt
    21781 gatttaaatg aggaacaaaa agagcaattg gaacgtctag tagataagct agaagacgat
    21841 gatgatgttt cagaagtctt tactgctgct gaaatgtaag tagcacattg ttagataata
    21901 aaaagaaagc acctgtacca atcgtacggg tgctttcttt ttatatatta tttttgttag
    21961 aagaccaata caggcgttcc tttttcgacc atcccgaata attccttcat tacacttggt
    22021 ggggtgttga tgcagccgtg tgaaccgcgg gttttccata agtctccacc gtattcaggt
    22081 tgccagtctg aatcgtggat accaacacct gtccagtcaa tcggcatcca gtagtttacc
    22141 ggactctcgt aaggtgttcc atcgtcattt gttcctttta aagttgcatc ttcttcttta
    22201 ttccaaacat agaagactcc agcaggtgta ggtgtagttg gctttccgga aacgatatct
    22261 gtttcaaggg ccacttttcc gtctttgtaa taccacatat gctggttctc cagatcaact
    22321 tcgatatacg tatcttcgat caatggatga tcagcggttg tgccaccttg cacaatgggt
    22381 gaacgagtaa aatcttggcc tgccagaatc gcttttttca atgcttctgt ctcactgtct
    22441 gtttggatcg tccaactgta agtgccaact gggaccgtaa cttctcctct ttttgtactt
    22501 ttaaattttg tgtcattcgt acttgtgtta tactttgtgc ctaaatctgt tacatattga
    22561 cgtacttgtt ctgtatctaa atcgactttt ccatcattgt aggtcagcca actcataata
    22621 tcacttgatg ggatctggaa agtctcaccg tttatactgt aagtagcttt tacatttgcg
    22681 atagcattca tggatgcgag ttgttctttc agtgagctgt cttcagatgt tacttttggt
    22741 ttttctttga attcagtcaa ttcaattgta tctttaccag agtttaccgc actttttaat
    22801 tgtttgaccg cagcatcgac gtcgatcgta tttccattga cttcaggctt gataacgaaa
    22861 gagtcacctg ctttttcgat cgttgcatca gtagtaggtg tccggtcttt gttcaaatca
    22921 gtcagcttag ttgtcaatgt ctgaacggta gtgtctagtt tttggtggtt ttgactgaca
    22981 ggatctattt cttgtttttc tgccgctgag acataagtca ttccccaagt ccatccgttt
    23041 tgttcattta ggatcggaga aagctccgaa gtgaaatcat ctttgtagcc gagctcggat
    23101 tttggtactt cctgccagac ttttccatct tctttaatag ccagtttttt atcagaataa
    23161 gcttctttta atttttcatt tgctttttcg actgttagat tgctgacatt tattccattt
    23221 atttctgtat tcggcaaaaa atgctttgtg tagtatgtac tgcgataggt ataaaagctg
    23281 atcagcacta agagaacacc aagaactgaa agcaaagcaa catgtttcga tttttttcga
    23341 tgcgaagatc ttgtcatatc gacaactcct taagtaaaag tttagggtgg aacggtctca
    23401 tgatagcata atttagagga aaagacgaag gttatctcta ctttatgaaa aaatttagaa
    23461 accataaggg tgataaaata aaaaaacgga aaacaccgaa tagatgtttt ccgtcatgtt
    23521 caaagattat tcagcgtcta gtttagcaaa agcttcatct agaatttctt ttaattgctt
    23581 agcagaagca gccatacggt cttgctcgct gtcacttaaa ggaatttcga ttactttttg
    23641 gataccttga cggttgataa cagcaggtgc accaatgtag atgtcgttca atccatattc
    23701 gccattcatg aatactgata aaggaagaac agcattttca tcatctaaga tggcacgagt
    23761 gatacgtgct aatgctactg cgataccata gaaagtagcg ccttttttct cgataattgt
    23821 gtaagcagcg tcacgtacgc caaagaatag gttaaccata gcttcttcat cgatttctgg
    23881 gttgtttttt acccattcat agatttgcaa gccggcaaca tttgcatgtg accaaactgg
    23941 gaattctgaa tctccgtgtt cacctaagat gtaagcatgg acgttacgtg catcaacgtc
    24001 aactaattcc gcaagtgctt gacggaaacg agctgagtca agagaggtac ctgaaccgat
    24061 tacgcgttct ttagggaaac ctgagaattt ccaagttgag taagtcaaaa tatctactgg
    24121 gttagcggca actaaaaaga taccgttaaa tcctgattca acgatttgtg tgacaacatc
    24181 gcggttgatt tttaggtttt tgtttactaa gtctaaacga gtttcacctg gtttttgtgg
    24241 tgcgccagct gtaataacaa ctaagtcagc atcatgtgcg tctgcgtaag tagcagaata
    24301 gattttttta ggtgaagtaa atgccaaagc gtgagacaag tcaattgcgt ctccttcagt
    24361 tttagcagta ttgatatcca caatacctac ttcttgagcg atattttgag taactagagc
    24421 aaaggcatag ctagaaccta cggcgccgtc tccaacaaga ataacttttt gatgatcttt
    24481 tttttctgca gctgcagtca tttagtatca atccttccat tgatttgttt tttcccatgc
    24541 ttatgctaac atttctcaag ccccatgtca cgctatttgg ctataaaata taacagataa
    24601 caaggttgta ttatatgtaa a
//
LOCUS       DAZULY010000012        24285 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000012 DAZULY010000000
VERSION     DAZULY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.012"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..797)
                     /locus_tag="ACG81A_000353"
     CDS             complement(<1..797)
                     /locus_tag="ACG81A_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5013751.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSE"
     gene            complement(1021..2961)
                     /gene="thrS"
                     /locus_tag="ACG81A_000354"
     CDS             complement(1021..2961)
                     /gene="thrS"
                     /locus_tag="ACG81A_000354"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFS5013752.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3298..3477)
                     /locus_tag="ACG81A_000355"
     CDS             complement(3298..3477)
                     /locus_tag="ACG81A_000355"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFS5013753.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3524..5347)
                     /locus_tag="ACG81A_000356"
     CDS             complement(3524..5347)
                     /locus_tag="ACG81A_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5013754.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5625..7904
                     /gene="helD"
                     /locus_tag="ACG81A_000357"
     CDS             5625..7904
                     /gene="helD"
                     /locus_tag="ACG81A_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFS5013755.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(7997..8644)
                     /locus_tag="ACG81A_000358"
     CDS             complement(7997..8644)
                     /locus_tag="ACG81A_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013756.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8823..9542)
                     /locus_tag="ACG81A_000359"
     CDS             complement(8823..9542)
                     /locus_tag="ACG81A_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5013757.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9673..10824)
                     /locus_tag="ACG81A_000360"
     CDS             complement(9673..10824)
                     /locus_tag="ACG81A_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFS5013758.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11041..11781)
                     /locus_tag="ACG81A_000361"
     CDS             complement(11041..11781)
                     /locus_tag="ACG81A_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5013759.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11785..12108)
                     /gene="rsfS"
                     /locus_tag="ACG81A_000362"
     CDS             complement(11785..12108)
                     /gene="rsfS"
                     /locus_tag="ACG81A_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFS5013760.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12165..12758)
                     /gene="yqeK"
                     /locus_tag="ACG81A_000363"
     CDS             complement(12165..12758)
                     /gene="yqeK"
                     /locus_tag="ACG81A_000363"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFS5013761.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12742..13392)
                     /locus_tag="ACG81A_000364"
     CDS             complement(12742..13392)
                     /locus_tag="ACG81A_000364"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFS5013762.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13402..13713)
                     /gene="yhbY"
                     /locus_tag="ACG81A_000365"
     CDS             complement(13402..13713)
                     /gene="yhbY"
                     /locus_tag="ACG81A_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFS5013763.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13729..14838)
                     /gene="yqeH"
                     /locus_tag="ACG81A_000366"
     CDS             complement(13729..14838)
                     /gene="yqeH"
                     /locus_tag="ACG81A_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFS5013764.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14835..15362)
                     /locus_tag="ACG81A_000367"
     CDS             complement(14835..15362)
                     /locus_tag="ACG81A_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFS5013765.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15542..16327)
                     /locus_tag="ACG81A_000368"
     CDS             complement(15542..16327)
                     /locus_tag="ACG81A_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFS5013766.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16320..17189)
                     /gene="accD"
                     /locus_tag="ACG81A_000369"
     CDS             complement(16320..17189)
                     /gene="accD"
                     /locus_tag="ACG81A_000369"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFS5013767.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17190..18569)
                     /locus_tag="ACG81A_000370"
     CDS             complement(17190..18569)
                     /locus_tag="ACG81A_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFS5013768.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18577..18996)
                     /gene="fabZ"
                     /locus_tag="ACG81A_000371"
     CDS             complement(18577..18996)
                     /gene="fabZ"
                     /locus_tag="ACG81A_000371"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS5013769.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19021..19497)
                     /gene="accB"
                     /locus_tag="ACG81A_000372"
     CDS             complement(19021..19497)
                     /gene="accB"
                     /locus_tag="ACG81A_000372"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFS5013770.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19500..20735)
                     /gene="fabF"
                     /locus_tag="ACG81A_000373"
     CDS             complement(19500..20735)
                     /gene="fabF"
                     /locus_tag="ACG81A_000373"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFS5013771.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20754..21491)
                     /gene="fabG"
                     /locus_tag="ACG81A_000374"
     CDS             complement(20754..21491)
                     /gene="fabG"
                     /locus_tag="ACG81A_000374"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFS5013772.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21503..22420)
                     /gene="fabD"
                     /locus_tag="ACG81A_000375"
     CDS             complement(21503..22420)
                     /gene="fabD"
                     /locus_tag="ACG81A_000375"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS5013773.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22527..22751)
                     /locus_tag="ACG81A_000376"
     CDS             complement(22527..22751)
                     /locus_tag="ACG81A_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS5013774.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22804..23766)
                     /locus_tag="ACG81A_000377"
     CDS             complement(22804..23766)
                     /locus_tag="ACG81A_000377"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFS5013775.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23776..24216)
                     /locus_tag="ACG81A_000378"
     CDS             complement(23776..24216)
                     /locus_tag="ACG81A_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5013776.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6638 a         5417 c         4236 g         7994 t
ORIGIN      
        1 ttttcgctaa gcttttcgtt tttagccata tagaaataaa aaatgatatc gatcgtagca
       61 aattgggcca aaagtgaatt agttgccgca cttcgattcg tgatttcttt tggtctagat
      121 gtttgtacga gtacatcggc tatcttgcta agcgggttat ttccaaattg cgtcaacgca
      181 atgatttcga tattcatgct tttcgccagc tcggctaatg cgactacatc tgcacttcta
      241 cctgagtttg aaacaagcca aagcactttc tttttctcat tgctgacaag ttgcgggagc
      301 aagacgtgga tatctttttc aaaaatgatt ggcttaccga cacgagacca tttttgcaga
      361 atatcttcta ctactaaaga agaggcgcca accccatata aaaaaacagt ctctgccgat
      421 tccaaacact ctacgacttt ttggatcgtt tcttcttcta gaatatctac tgtttctttt
      481 atcgctactt gtgcattgtt accaagtttg tttttgatag agtaaaagga ctcatttgct
      541 tctatatcac taaaaccttc atcgattcca gtctgtagtt ctgttgaaat cgtgattttg
      601 aagtccgtca gactttgaaa accgattttt ttggaaaaac gtacaactgt cggcgcactc
      661 aacccggaag cttctgctaa ttcattagct gtcattgtag ggaccttctc aatattaccg
      721 ataatgtagt tggccaactt tttttctgct gaagaataat cttccatcat actttcaata
      781 cgactaatta cgctcataag ttcctccacc cttttaatga tagtctatgt aagtatacca
      841 tatcagaccc ttattcacgg tcgtatactg gataaaaaac aaaaaaacaa tccgcccaac
      901 agaatatctt atcccatcaa tcccacagat tctattgagc atctgtgtct ttgaggaata
      961 cctgttatct attgttcgga ttgctttcta tctactactt ttttacagga aatttaccta
     1021 ttagttttct ctgctgtaat ttttgacttc tgcttccata gcatcaataa agatattcaa
     1081 atcttctaca gctgtttctt tacttccata acgacgtacg ttcactgttg catcttccaa
     1141 ttccttgtct ccaacaacta attggtaagg gatcttttgt gtttgagagg cacgtatctt
     1201 atagcccatt ttttcattac gatcatcaac ttctacacgc aaccccttca tttgcaaacg
     1261 ttctttgatt tcataagcat aatcgccatg tgcatcaaca gaaacaggga tgattgttgc
     1321 ttgtataggt gccaaccatg ttggaaaagc gcctttgtat acttctgtta aataagcgac
     1381 aaaacgttcc attgtagaaa caattccgcg gtggataact actggacgat gcgtattttc
     1441 cccgtcttct ccaacatatg tcaaatcaaa gcgctctggc aataagaagt ctaattgtat
     1501 agttgataat gtttcttctg taccaagagc tgttttgact tgaacatcta gttttggtcc
     1561 gtaaaaggct gcctcgcctt ctgcttcaaa gtaatctagt tctaattcat ccatagcggc
     1621 tttcagcatt gcttgggctt tttcccacat tgcgtcatca tcaaaatatt tatctgtatt
     1681 gtttggatcg cgatagctca agcggaaacg ataatctgta atattgaagt cagcatatac
     1741 tgccaccatc aattcaagcg tacgtttaaa ttcatctttg atttgatcag gacgaacaaa
     1801 tgtatgacca tcattcaacg tcatttctcg tacacgttgt aaacctgaca aagcacctga
     1861 tttttcatag cgatgcatca ttcctaattc agcgatacga attggtaatt cacgataaga
     1921 atggatcgtg tttttgtaga ccatcatgtg atgagggcag ttcattgggc gaagaacaag
     1981 catttcgcca tctcccatat ccattggcgg gaacatgtct tcttgataat gatcccagtg
     2041 tccagatgtt ttgtacaact caacatctcc cattattggt gtatatacat gttggtaacc
     2101 tagactaact tctttgtcta cgatataacg ttcgatcgtg cgtcggattg ttgccccttt
     2161 tggaagccag aatggcaaac cagaacctac ttctggggaa accatgaaca agtcaagttc
     2221 ttttcctagt ttacgatggt ctctttcttt tgcttcttca cggagacgga tatattcttt
     2281 caaagctttc ttgtcaaaaa aggctgtccc gtatacacgt tgcatcattt gattgtttga
     2341 gtttccacgc caataagcac ctgccactga taataattta aatacttgta tacggccagt
     2401 ggatggaaca tgtggtccac gacacaaatc gatgaattcg ccttgttgat aagcagtaat
     2461 cacttcttct tctggcaatt cactaatcag ttctactttg tacggatcac ttgcaaaaat
     2521 ttctaatgct tcctgttttg aaagaacacg gcgttcgatc ggcagatttt ctttgacgat
     2581 tttcatcatt tctgcttcga ttgccggcaa atcttctgca gttacttggt tttgtccatt
     2641 atctgtatcg tagtaaaacc ctgaatcgat agctggtcca acgccaaagt ggatatttgg
     2701 atagaggcga cgcatggctt gggccatcaa atgagctgct gaatgacgga ctaagccaag
     2761 tgcatcttca tggtctggtg tgatgatctc aatacttgca tcttcctcga ttggacgagt
     2821 caaatcaacg agttttccgt tcactttacc agctaaagct ttttttgcta agctttttga
     2881 aatgctttca gcaattgcca atgttgttgt tccagactca aattctttga ctgcaccatc
     2941 tggaaaagta atttttatca taatagtttc ctccttgaaa tgttttgatt gatcagatca
     3001 tcaagctgaa aaacaaaaaa atcctagcat ctcttgaaaa gagatactag gacgaaaagt
     3061 tcgtggttcc acctaatgtt caaacgcaaa tgcgttcctc aagcgttgta acggtccgac
     3121 cgcccttgct tcaacaaggg tttcgaaagt ggtcatcctt tggttttctc ctggaaaagt
     3181 ttcagcctag cttttcctct cttatcgaag actctcaaat tcagttggct ttctcatcaa
     3241 tcaattatgt gtttatttaa ccactaataa atagaaaaat caagtgggaa actaaactta
     3301 ttttttccat tccccattta caccgtatgt tccttttttc atctcattga tgatgacatg
     3361 gatgtgttct tttggtgcct gcgtgttttt atgaacagct tccgtgatat ctttcatcat
     3421 attttctaat tgttccggac tgcgaccttc tactaattcg acatgtacga atggcataat
     3481 gctctctcct ctttctgatc gttctttttt caactcgatt ctatcatact atgtttgtct
     3541 tgggtactga atcaggaaaa atggctggat ctttttttgc tctcaaggca ctcaaaatag
     3601 agaccgtatc cacgacttct tgcaaaacag ctccgaataa tgcggggatt agtcccgtac
     3661 ttgcaattaa catcaaaaaa atacaaataa agatgccaat cagtacagac tgtttcgcta
     3721 ttctcatcgt atctcttgaa atcactactg cttttgctac acgcaagaga tcatctttca
     3781 agatgaccgc atctgcactt tcactagcag ctgtagaacc atgagccccc attgcaatac
     3841 cgatatctgc agcagcaagt gctggtgcgt cattcacgcc gtctcctacc atcgcaacag
     3901 gtctttgatc tttagggata ctttctaaaa tggtcagttt atcttgaggc aagctacggg
     3961 catgtacaga atctaattgg actttttctg caatcgtttg agcaatggca gatccatccc
     4021 cggtgatcat catcaaattt ttcacatcaa gttttcttag ctccttgatt gtctttgctg
     4081 cttctgggcg aatctgatcc gaaaatagga tcgttcctgc aaaagatcga tcaattgtta
     4141 catagattgc tgtattggac gtttctattt cttcagctga aacaaaagag gcacgtccaa
     4201 tctttacttc atgattattg actactgctt ggattccttg accggtcact tctttcagtt
     4261 gactaacagg atacagttgg ctgtttttca cgttgttcac taacgaacga gctaagatat
     4321 ggctagattc ctgttctgca ctagctgcat acagaagtag ctcttctttt tttattccat
     4381 tgactggctg aatcgcctct acttccaata tccctttggt aagcgttcct gttttatcaa
     4441 aagcaatcgt ttttgtacgt gcgagctttt caatcgttgt tccagttttt acaacgatcc
     4501 cgtttttact tgaacggctc attcctgcaa ctaaagcaat gggcgcagct agaatcagtg
     4561 gacaaggtga tgccactacc agtacctcag caaatcgtaa agggtctttt gagaaccacc
     4621 aagaaatacc accgatcaaa taagcaatca acgtgaaagg gactgcataa cgatcagcta
     4681 aacggacaaa tcgggcaggc ttttcttttg attcttcgac tagtttaata atcatttgat
     4741 actggctgtc tgcggcacgt ttttcagcga tcatttggat cgatccgtct ccatttaaag
     4801 accctgacat aatctgatct ccaggtttct tggtttccgg gcgggactca cctgtcaaag
     4861 aagattcatc gaccgaagat tctcctttgg tcactcgacc atctgcgggt acaagttcgc
     4921 caggcttgac tagtaactgg tcatcaatat ttatttcatc gattactata tcctgcaatt
     4981 ttccttcttt gaatcgatgg gcaattcttg gggtgttatc taacaaagac tgcagttctt
     5041 tacccgctcg tttattcgcg taatcctcta gactatctcc gcccgtaagc atgatcaata
     5101 tcatcaaact agcccagtat tctttgacgg ctaatgttgc aaggattgcg gtaatcgcta
     5161 atacatctac gccatatctt cctgaacgta acgtcttgat catttccaaa aacatcaaga
     5221 aggcgagaat ccctccagta aatgcgacaa tcgcaaatgc aagggccggc tggtgaaaaa
     5281 caaattcact taataaagct atcccaccaa taagcaaagt aagtaaaaat tttttgaaat
     5341 gtgtcattgt tttttctcct cttttatgtg acttagagag gttagtaata aatttccagc
     5401 tgcctctttc atcttaagca tagtccttga ctagcacttt ttaaaacaat aggccttaat
     5461 tgaaaacaac tatcactatc agcgtttcca aaattcatca gttctacaaa caaaaatatt
     5521 cagcaaggat tactttttcg attcatcagt taaaaagtac cttttttcga aaaactatgc
     5581 tatactttta attcgttatt tacggcggaa ggagcaaaca tgcgatgaat gagaaacaac
     5641 tagaacaaac gcacgtggat gaaacgatcg aactaatcca gatcgaacaa aatatgctgg
     5701 atacaaaaca acaagaattg actcaggaaa tgaatcattc gacaaaagac acagctaatc
     5761 acaagattcg agctggttcc aatgaagctt tttatgaatc tgctgttgaa tatcgccaac
     5821 atgagcagga attgcttttg aaataccata ctcttgaagc acagcaaaaa cgcctgcaaa
     5881 cgttgtctgt catgaaagat agtccttatt ttgcccgtat cgactttaaa gaagaccgtg
     5941 aaacagaaac attatatcta ggtatcgctt ctttaagaga tctggcagaa gaaacaatcg
     6001 ttattgactg gcgcgcacca attgccaatc tttattatga aggtgagatt ggtcccgcat
     6061 tttatgaaac tgaaacagat agaatagatg tcgatctgca gttgaaacga caatttaaaa
     6121 tccaagaagg acgtatttta tctatggtag atacgtcgga agtaatcaat gatgactttt
     6181 tgctagagat attggatgaa tcatcaagca accatatgaa aaacatcgtc tctacgatac
     6241 aaaaagcaca aaatgcaatc attcgggaca caacaagtaa agtaatgctg attgaaggaa
     6301 tcgccggaag tggaaagaca tccgctttgc ttcaacgtat tgcttttctg ttgtatcata
     6361 atcgcaagtg gctcgaggct gaaaacgttc ttctattttc acccaaccat ttgttttctg
     6421 attacatctc gactgtattg ccttcattag gtgaaagcgg cgtgcctaca cagacattca
     6481 aaaattattt ggtgcagctg ctgccagagt tttctttact agatgaacag caacaagaaa
     6541 gtggtttttt gctaggggaa aaagatccga tacaagtgat gaaaagcgga ttgaccttag
     6601 tcgatcagat tgatcgctat atccaaggca tcacaaacta cggtcccttg ttccgtgata
     6661 tgaaaataaa tggccgtacg atcctatcaa aggaatcgat tcgccaatgg tataaagaaa
     6721 caaatgaaca attaccttta catcatagac tttctctatt acaaacgaag ctcctgaaaa
     6781 aattaggcgg tctgcaaaaa gacgaaacac gtcagcaatg ggtcaaagat ctggcggaag
     6841 aacagctcca agaactatac gcaacagatc caaatcttga atatacagaa caaaaggagc
     6901 gttcattacg taaaaaactg gccaaccaaa tcgtacgtaa aagattccgt cgaatccatc
     6961 gagggatcat gaattatcaa tttgtcaata taacgaaaca atatctccac tttctacaaa
     7021 ctgtgccact ttctcttcta acacgacata aaatcagcga gaccaggtgg caggaacatt
     7081 tgacaactgt tcgtacacac ttgcgtcaaa gagaattgcg tcaagaggat gctgtactat
     7141 tctttttact gatgcgccga acatatccag tgacggttac acacaaagct cgctatatct
     7201 ttattgatga aatgcaggat ttcgcaccat cacaagtggc attgctgcaa gccttgttcc
     7261 caaaagcaaa tttgactttc tgtggagatc taaatcaaaa tgtctttgga aatgaaacga
     7321 tcgttggatc attggaagac cttttcgtgg accgtgaagt cactcgtttc caattaacga
     7381 caagctatcg ctccacaaaa gaaatcactg attttgctaa tcattttatt gtgaaagaac
     7441 atcaagtaga aacaacagca agaaaaggag accttccttt gctcatccaa ggaacaacag
     7501 atgatatcaa attgaattgg ttagcagata ccattcgaac gactcaggga aaagcccggt
     7561 attggcgtac agcaatcata gctaaaacac aagaagaatg cgaagctctt tatgctcaac
     7621 tgccagacga gttgaaacaa caagtccagc tgattttaaa tgagagtgac tttatgaagc
     7681 gttccattat tttacttcca gcctttttag caaaaggtct ggagtttgac cgagttttct
     7741 tgtgggatgt agatgcagaa catttttctt ctcagcaaga caaattgatc ctttacacca
     7801 tgtgtacacg tgcgatgcat gaattgaccc tttttacctc caataatgaa agtcctttgt
     7861 taaacaatat ggatatttca tcttatgaga aaaaagtact gtaagtaaaa agacaactgc
     7921 tatcattaat gttagcagtt gtctttttat ttacaataat aattgcagaa aaagtaattc
     7981 aatcatttaa ggtcatttat tctatatata aatccaaatc tgtttcagga tattttaatt
     8041 tgacaagcga aataatcatt ttcagttctt ccatgttttc tggatattct gggtcaaata
     8101 ccttgctatg acggccaatc ccgccattgt cgatcaatcg gtaaattcga ttccattcaa
     8161 tgaattggtc aattgattgg aaattgctga ttggatagcc gccacaatcc aaaatctctg
     8221 atacacgaaa tgctaggtat gcactatcga tcactttatt ttttttcgtg tctatcttct
     8281 catttctttc gataagaatt ttttcaggac gatgtttcaa gctccactct aatagtagaa
     8341 agaaagcctc catccgatac tcttcttcaa ataaccgaaa ggctgaatca gaacgacgaa
     8401 aaagcagcgg aatcagataa ttttgtctat aaatcagcat tggtgcgcgg tccaccatac
     8461 actctaaata acggttcaga taccctgtac cgacatagtt tcctaatcga tagccttcaa
     8521 cgccgaatct tgcgtatttt tgcgctaatt ttgaacaatc agttacgttc acagctaact
     8581 cttctacttt gcggtaaccg cttaagatac gttcaaaagg ttctaaagtg atttttgctt
     8641 ccatattctt ccccgtccat cttccacata cttcatatca tttcacatga tacttatgct
     8701 ctcatcatac cacaggttga attagaaaag aaaataaatc acacaaaaaa gcaagacaat
     8761 ctgttagtga gattgtcttg cttttcgaaa aagactgaat acttttcgtt aaaactggtt
     8821 gcttagtctt ctaatgccac attatggaac acatgctgta catcttctag atcttccagt
     8881 gcatcaatca ttttttcaaa tgtttcttga tcttctggag atagtgttac ctcgttttga
     8941 gggatcattt ggatctcagc gattgaaaaa tcagtgattc ctttttcttt taatgcttct
     9001 tgtatactat ggaaatccgc tacttcaccg taaacgatga tttgtccttc ttcttccatt
     9061 acatcgcgaa catcgatgtc tttttccatt aggtattcaa ggatctcgtc agcgtcttct
     9121 cctgcaaaag caaagattgc tgtattgtca aacatgtaag aaaccgctcc ggaaacaccc
     9181 atgttcccgc catttttccc aaatgctgcc cgtacgtctg ctgctgttcg attcacattg
     9241 ttcgtcaatg tatcaacaat caccattgag ccgcttggtc caaatccttc atagcgcaat
     9301 tcagagtatg tttcatctcc tgcacctttt gctttttcga ttgctcggtc aataatatgt
     9361 tttggtacat tataagtttt agcgcgatca atgacgaaac gtagtttttg gtttgcatgc
     9421 ggatcaggat cacctgattt tgctgccgcg tagatttcaa tcccaaattt tgcataaatc
     9481 cggctattat tagcatcttt agcagttttc tttgctacga tatttgccca tttacgtccc
     9541 atattttcac ttcgctttct gatttgatag tttcataacg caaaaacgtc ctgttccatt
     9601 atacttgtga atcacgattc tgtgaagctt tttttctttc ttccttcatt cttctcattt
     9661 ttagtcaatt atctatactc ggattgggat ttgcccgaaa ttctgttccg gaatattgcc
     9721 tcccaattga tagatttgat cacttctaac taacagttcc gcttcttgtg cctctttttt
     9781 tccgattcgg gaataaatag aaatctcttt tttttctttt aagaatcttc ttccagcatc
     9841 ggtaaatcca agtagtcgaa gataattttt attttgttgg acaggaatct cttgcttttt
     9901 gatattcagc aagacataac aagataatcg ttggatccgt gtccatgtat atctttttgt
     9961 ttttaccttt gacacaaaat cctcaaaact atcagcagtt gcagctgctg ttttcatgcg
    10021 cgattctatt ccttcggtca tctgatagat atctgtcagt tccagcaaag tagaagataa
    10081 caaccgataa cgcaaatacg gccaataaga ttcccaagtc tgcagcgaac aagttgaaag
    10141 atcttcttct gtctgaggcg gcacaagtcc agctattctt tttccttctt ctaacgcatg
    10201 gcgaatggct gttgcactgg ctatcctctg atctgtgatg attttttcat gatagcctgc
    10261 acttttacgt cggatcggat gtaattccat agggtggaca tacttggcat tttcctttgc
    10321 ataacttaat cccaagatat gatttggcga agaaaaatca agcgtaatat cgggatacag
    10381 actgcgaaaa acctcagtca ttttttgcgg ataactatac gtttgatcag acagttcttg
    10441 aaatgcctct tctatcctat cttgattctc ttggacaaag cgtccgaaac tgctgtaatc
    10501 aaacgaagtc tcttcttctg ttccaaaaca caaactgtca cagtccagtg tttgcaagat
    10561 ttttactgca cctttagcaa aatagtcagc tgactgaacc gcccattcga cgggaagctc
    10621 cactactaaa tccacaccat tttgtaaagc ttcattcgcg cgatgccatt tatcgatgat
    10681 agccggttcg cctctttgca aaaaattccc gctcattaca gcaatgacga cctctgctcc
    10741 tgttattttt cgtgccattt ccacatgata gcgatgtcca ttgtgaaaag gattgtactc
    10801 tacgataatc ccgcagcttt tcatgcgcat cctccttcat agttatcgat aaaaaagaaa
    10861 tagagacaag taaaaaaagg caaaaatcgg aaaaaatacc cgaactatat aaactattct
    10921 cgcaaacaca atacttctgc gacgaaaagc tgcactgcaa gagcaatgcc tatatcgttc
    10981 ggatatataa tttatgttga agatttattc ttttcttgtt aactcgagca ggcaaaaccg
    11041 ctagtctttg tatgctacaa agaaccagcg ctttgatttc tctgatgctg cttcgtctgt
    11101 aaaatcagaa aatgcttcga ctttcgaaaa gccgctgttt tccagcattc ttaaataact
    11161 ttccaatgga taggtacgct cttgatgtag ttcatcttca cgaataaatc gaccagaccc
    11221 ttcttgttct tcaacgaaaa aggtaagaaa atgttcgatg ctgtgtttct cgcttcctgg
    11281 ataactatcc cacaaaaaag caaaattatc cccttgataa tgatagctat attctggaaa
    11341 aacctcatct acttgatagg tggagtgtac gtcaaataaa aatcggccat tttcttctaa
    11401 cgcttgatag acttcatcaa acacttgctg tacctcttgt ctatttttca tatagcacag
    11461 cgaatcagaa aaacaggtaa ttgcctgata ttgtccgatt tctgataaat cgagcatatt
    11521 gccttccaca aattggatcg atgcgtcttc ttcatatgct cgctggctgg caatcatcaa
    11581 catctcttct gaaaggtcta accctgtcac tttatatcca tcttttgcaa aggcaaccgc
    11641 taatgctcca gtaccgcatg ccagttccaa aatatttttt gtgtcttctt gtaaatgacg
    11701 tttcgaaaaa gctaaccatt gctgataaag tgtttcgtcc atcacttcat cgtaaatgaa
    11761 agcaaatgtt tcataagcca tatgttaatc caaccattca ctaagatcga ctagcggagc
    11821 atctgaccat agtttttcca gattatagaa atttcgttct ggcgcatgga aaacatgtac
    11881 gatcacatct ccaagatcga tcaataccca ttttccgcct tcttttcctt caatacgttt
    11941 tacttcatat ttgttttctt cttctttatc aatgatttct tctgtaattg cgttgatttg
    12001 acgttcgcta tttgctgaac agatcatgaa ataatctgcc aacaaggaaa catttcgaac
    12061 gtctaaagcg ataatgtctt ctgcccgttt tgaatctgct gcttcaatcg caatttttaa
    12121 ttgttcttta ctatctatga tgatttcctc ctaatatgag cagcttattt ttttgctacc
    12181 cagtaattat atgtttcgat tgtttttgga taaatctgtt gttcttgcgc aagcaaatgg
    12241 gccaatgtat gttttgtttc aaatgctaca gcttcgtcta gatcagccca tgcaatggct
    12301 cgtgcatctt gtaccccagg aaaatctcga cctggttcaa tatagtctgc tacataaatg
    12361 attttgtcaa ggagactcat cttagcagcc ccagtcgtat gcacccggat tgcatgaaga
    12421 atctctgcat cggtgattcc taattcccgc tctacaaacg aagcgccaac aagaccatgc
    12481 cagattgcat tattataatt caataactcc ggatcaaaac cgtctcttac gattaccatt
    12541 ttgaactcct catctggtcg ttcttttgca taatcatgtg tcaatgcagc aatactagct
    12601 ttttcaggag aagcaccgta ttttttggca agagccacgg ctgtttcttc tactccaagc
    12661 acatgcttga agcgttgttc gctcattctc atctgtattt tttgcatcag gttttctcta
    12721 gaataagcag tatattcatt actatagtcc atattcatac aacccttcat tttgaatata
    12781 atcaattact ttatctggaa caaggtagcg gatagaacag ccttctttga tcttttgacg
    12841 tattttcgtg gaactgatgt cgatctcagg tacatctacc caaattacag gatatggagt
    12901 atccgttgtg tatcctggtc gacgtatacc cacaaagttg accatagaag tcagctcatc
    12961 gattttatac cattttggca aatattctac catatcccca ccgataataa agtaataatc
    13021 tgtatccgga ttgttttgcg tcaattcctt catagtatca taggtgtaac ttttcccacc
    13081 acgagctaat tcaatcgttt caatctgcaa aaaaggattg tcttccaccg ctaattccaa
    13141 catgttgagt cgatgttttg catcaatcgt ctgtttctca tcaacatgag gtggctgata
    13201 agaaggcatc aaaaatacgc ggtcaagacc taaattccgg ccagcttgtt cagccatcac
    13261 taaatgtgca aggtgaacgg gattgaatgt tccgcccaag atccctactt gctttttctt
    13321 ctctaagaat tgaggcattt cttcaggaaa taattgtgaa cgaacaaatg tcttagcttg
    13381 cgtattcatc atggtcactc cttatattgc ttttacttct tttgatagcc tttgatactt
    13441 ttcttttgtg gaaggtttgt ataatacaat aacacgtcca atgatttgga cgatttcgca
    13501 atctatttct ttttctaata cttgagctgc ttcttctgca atctcatctg tattttgaag
    13561 taacgatact ttgatcagtt ctcttttttc taaagcttct tcaatttgaa cgatcattgc
    13621 tgaattgatc ccaccttttc cgatttgaaa aatcggctga agatgatgcg ctttgctacg
    13681 taaaaaacgc ttttgttttc ctcttaaact cataattttc cttcttcctt aaatcaatgc
    13741 tttgcgtttc aatacatcta caccctttgg cgcccagccc gcaattacta ctggcgctgt
    13801 tacagtgatc cagcctagac cggcaaaaac gatgtctgtt ttttctttca cagaaaactc
    13861 aaaacgaaca agctccggaa atgcctcgac ttcatcagga cgaggaggct gtaataatcc
    13921 acctacatgt ttttgataaa attcgtctgc tttttccagt ttcgttcgat gaatatttac
    13981 atcatttgac acataagcaa taaatgaagc acgattgccg ctgataaaat caaatcttgc
    14041 aagtccgccc aagaaaagtg tttgcccttc attcagctga taaactttag gtttgatttc
    14101 ttttaacggt gcaatgatcc gcaaatcttt tttacctaag tagtgcgcca tttgatggcg
    14161 atggataatt cccggtgtat caatcaaaaa atgcccgtca tctaaaggaa tctcgatttt
    14221 atccaaggtc gttcctggaa actgcgaagt tgtgattaca tcttgtactc ccgctgtatt
    14281 tttgatgatt tgattgataa gcgtcgattt tccgacattt gttactccga cgatatagac
    14341 atcccgtcca tcgcggtatt cttcgatttt ttctaatagt tccgtcatct catgagattt
    14401 cttagcacta gttaataata catctactgg acgaattcct gcttcgtaag cacgttcctt
    14461 catccattgt gttaatttag tttttttcaa tgatttaggt aatatatcca ctttgttccc
    14521 aactaaaagt actggattgt caccgacaaa acggtgtagt cctgggatta gtgaaccatt
    14581 gaagtcaaaa atatccacca cattgacgat caatgcatct ttttgcccta attcattcag
    14641 taagcgcaaa aagtcgtcat ccgtcaatga gacatcttgg gtctcattat aatggcgtaa
    14701 tctgaaacaa cgctgacaat agacttcccc ggaagctaaa cctttttcca aagccgtttg
    14761 cggcgtgtat cctaattcac cgggtttgtt tgtttgtata acggcaccac agccgataca
    14821 ttgtaattct tctgtcattc gattccacct cgccagccca tatcagggtg ttttgctaat
    14881 agataggcca taattctttt ttcccaaaaa cgattaaatt gtgtcttcca actatctgtt
    14941 gcgataatgg gttttacaag gatgctacgt atccctgcac gattggctcc acgtatatca
    15001 gtcataatct gatcgcctac cattactatc tcatcatctg ctaaatccaa ttgtttcttt
    15061 gctatgttga tccctcttgc taaaggtttc attgctcgtg caacatacaa aagatcaaat
    15121 ttttcaatcg ctcgcgcgac acgttttgaa ttattgtttg aaacaacaac aacagggatt
    15181 cctgcatttt tcatttctaa aatccaagta agcaactctt ctgttccatc tggattattc
    15241 catgcaatca acgtattgtc taaatcggtc aatactgctt tgatgcctag attctttaat
    15301 tgggcaggtg taatctgata gatggcttct accatccatg ttggtttgta atttgaaaac
    15361 atgctgtctc ctcatctgca aaaaatgaag gagcacataa agtactcctc aaccccgtat
    15421 tatactgcat atcagcaaaa aaaactacct ctatactcgt tcgtctttca gaaaaagcat
    15481 ttctttacaa aatgaccaaa aataaagatt gatcacagaa aaatttagaa acaaaaaaca
    15541 actaatattg gcggaacctc ttgtatcgtt tttccagtaa tttatgctga tctaatcgaa
    15601 caagttccgt caatctttca atcaacgctt ttttcataat gtgtgcgact ttatatcgtg
    15661 gcaactgctc gccgttgaac gtttcaggaa taactcgatc aacgattcct aactcgcgta
    15721 gttctggcgc agtaattttc atcaactcag ctgcttctgc ggcgcgacta ccgtctttcc
    15781 acaaaatcga cgcaaatcct tctggagaaa gcacagcata aatcgtttct tccagcatcc
    15841 agacttcatc cgctaaacct agagcaaggg caccgccact tccaccttca ccaatgatga
    15901 tggcgatgat cggtaccttt aaatcagaca tttcgagcag atttctggca atagcctctc
    15961 cttcgccgcg ctcttctgct cctacaccgc aatatgcacc tgctgtattg atcaaggtaa
    16021 tcaccggtct attgaacttt tccgcttgtt tcatcagtcg taaagctttt cgatagcctt
    16081 caggatgggg agaaccaaaa tttctgcgga tattttcctg cagatcccgt cctttttgaa
    16141 taccgatcac tgttaccggt ttgccatcta atcgagcgat tccaccaaca atcgcttcgt
    16201 catctgcaaa taaacgatca ccatggcatt cctcgaaaga atcgaagatc tcttcaaaaa
    16261 aatccaagct tgtccatcgt tctttagctc ttgctaactg aacgacatca tgtgctgtct
    16321 tatccacgat ctatccatcc tttctgtgta tgcaattcaa gaagcgtatg gattttctgt
    16381 ttcaattcca tgcggggaac aatctgatcg acaaaaccat gagataataa gaattcagcc
    16441 ttttggaaat cttctggaag ttcctgcttg atcgtctgtt cgatgacgcg tcttccagca
    16501 aaaccgatca agctttgggg ttctgctaga ataatatcgc cttccattgc aaaactggca
    16561 gttactcctc cggtcgtcgg gtccgttaaa acagttagat aaaaaagacc ttcattgcta
    16621 tgccgtttga cagcagcaga gatttttgcc atctgcatta gagaaaagat tccttcttgc
    16681 atccgtgcgc cacctgaagc tgtgaacaag acgactggaa gactttcctt tgttgctcgt
    16741 tcgaataatc tggtgatctt ttctccgaca atcgttccca tacttcccat gataaaatta
    16801 ggatccatca ctccaataat gaagggaatc ccctgaatgg ttgctttacc agtgaggact
    16861 gcttcatgaa gtcctgtttt atcttgcatt tttttaattt tttccgtata ttcgggaaat
    16921 gctagcggat ctttggttac aagatccgta tcccattctt caaaactttt ttcatcaata
    16981 atcaaagcta gacgctgcca tgcaccgatt cgaaaagcgt atccgcaatg agggcatact
    17041 ttttcttcac cgatgtcttt cgtataaagg atatgtttgc aatttggaca ctttgcccac
    17101 atgttatcag gaacagatgg cgagttagca ggtttgctct ctctattagg attgatccga
    17161 atatatttct ttttcttaaa cagagccatc tatgcgcctc ctatctccgt ttctggtgtc
    17221 caattcggta aaaattcttc ttgtaaaaat gctgtgctgt aatcacctgc tatgactgca
    17281 ggatgactaa tcaaatccat ctgaaattct gcgttcgtga tgattccttc tgtgataaat
    17341 tcacttaagg ctcgctgcat cttcattaat gcttcaaaac gcgtatttcc atggacgatt
    17401 atttttgcaa tcattgaatc ataatatggc gggatcgtat atcccgggta aactgcactg
    17461 tctactcgca gtcccatccc acctgctgga agcagtaagt tttggatctt ccctggagaa
    17521 ggagcaaaat ggaaagcagg attttctgca ttaatccggc attcgattgc gtgtccttgg
    17581 aatactactt cttcttgaca cacattcaaa ggctcaccgg ctgctatctc cacctgtttt
    17641 tttactaaat cgattcctgt gaccatttct gttactggat gttccacttg gattcgagta
    17701 ttcatttcca tgaaataaaa atcgccttct tcatccatca gaaactcgat cgttccggca
    17761 ttttcataat tgactgcttt agctgctcga acagcagcgt ctcctagtgc ttctcttttt
    17821 gtttgtgaga tcactacaga aggagattct tccagtactt tttgattgtt gcgttgtaaa
    17881 gaacaatctc gctcgcctaa atgaatgaca tgaccaaatc gatcacctaa gatctgaact
    17941 tctatatggc gagcaggata aatgattttt tccaaataca tatcatcatt tccaaatgcc
    18001 gctgctgctt cttgctgagc tgaagaaaaa tgctgtggga gctcttcttt tgattgaact
    18061 ttccgaatgc ctttcccgcc accacctgca gctgctttca gcatcactgg ataaccaatt
    18121 ctgtctgcta tttccaatgc ttcttctact gtagataata caccagtact tcctggaatg
    18181 actgggacat ttgcttcttg catcaattga cgcgcattga tcttatttcc cattgcatcg
    18241 atcgttgcac ttttaggacc aataaatgtg atattgcact cttcacacat ggaggcaaat
    18301 cggctatttt cagataaaaa accaaatcct ggatgaattg cttcggcttt tgtcacgatt
    18361 gcagcgctca gtacttcctg aacattcaga tatgaatcaa cggcttttgc cgggccaatg
    18421 cagattgctt catctgctaa ctgtgtatga agtgcttcgg catctgcttc tgaatatatc
    18481 gctacggttt ggatccctag ttcgcggcat gcacgaatga tacgtacagc gatttcaccg
    18541 cggttagcta tcaatacttt agaaaacata tttcacctat ccaatcataa aggttaattc
    18601 cgcttcggct acttttttac cgtctacttt agcgattcct ttgcctattc cggctgaagc
    18661 tttcactttc aatatctcaa cttcaaggta taaggtatct cccggtgtaa ctttcttacg
    18721 gaattttgct ttgtccaatc cgccgaaata agccgtcttt cccttgaatt gttcaagaga
    18781 aagtaatgca actgctcctg cttgagccat tgcttcaaca atcaataccc ctggcataac
    18841 aggttcttct ggaaaatgtc cttgaaaaaa gggctcattg atcgttacat ttttcttagc
    18901 aacgatccgt tctccttcaa ccatttcttc cactcgatca atcaaaagca ttggataacg
    18961 atgtggaatg atttctttta tttcttgtat gttcattgtt ttccctcttt cctgctttgc
    19021 ttatttttgc tgaatcctaa agagtggctg tccatattcg acaactgact cgttttctac
    19081 aaggatttct gtgatcactc cgtcggcctc actagttatc tcattcatca gtttcatcgc
    19141 ttcaataatg cagataactt ctcctttggc aacttgatca cctacttttt tgaaagctgg
    19201 tttgtcgggc gctggttgca gataaacgat tcccacgatt ggcgaaacaa cctcgattcc
    19261 atcaaatact gccgattctt tttctttcat tatttctgga gcagttgttg cttgttcagg
    19321 tatgacattg actggctccg aaactggcaa cgtttcgttt gtttgaacca tattcatgcc
    19381 tcgacttgtt ttatttttat tcatatacaa ttcaaattga ccttcacgca aatcgaattc
    19441 cgttagcgag gattgatcaa attgactgac taattctctg atttcattga tatccaaaat
    19501 tattcctccc agcgtttcat gcacagaacc gcattatgtc cgccaaaccc taatgaatta
    19561 ctcattgcat aagtaaaact tgcaggtttg ctttcgtttt tgacaactgt caagtcaatc
    19621 tcaggatctt gtttttctac attgatcgta ggtgggataa attgatgttc taatgccagc
    19681 aaagttgcca aggcttcgat cccgccggct gctcccaata aatgtccagt catcgatttc
    19741 gtgctgctga cgtaagtatc tctgaaattt tcacctaatg cgatttggat tgcttttgct
    19801 tctgcctggt cattggctgg tgtacttgtt ccgtgagcat tgatatatcc gacttgttcc
    19861 ggctgtacac cagcttcttc catcgcaagc ttcattgctt tagatgcacc agaaccatct
    19921 ggagtaggag ctgtcatatg gtaagcgtca cagtttgcac cgtatccaac gatttctcca
    19981 tagattttgg caccgcgagc ttgtgcatgt tctaaagatt caagaactag aacgcctgct
    20041 ccttcaccca tcacaaaacc attccgattt tcgtcaaacg gaatcgaacc acgattagga
    20101 tcagttgatt ctgagagtgc tgttaaagct gcaaatccag caatccccat ctcacaaatc
    20161 gttgcttctg ttcctccagc aagaatgaca tcagaatagc catgtttgat gttacggaat
    20221 gcttccccaa ttgaattcgt tccgctagca caagctgtta cgatagctgt acaaatacct
    20281 tttgcaccta cacgcaaagc aatatttcca gctgccatgt tggcaatcgc cattggtacg
    20341 aacattggag aaactcgttt tgcacctttt tcatgtagac gaataacttg gttttcgatt
    20401 gttggaagac caccgatccc agatccgatg atgacgccaa accggtcttg attcattttt
    20461 tctttatcta aaccgctcat ctccattgct tctaaagcag cgtagattcc atagatcgaa
    20521 aacatatcca ttcgtttgct atctttttta ataaaatatt tatcgtaagg gaaatctttg
    20581 acttcacccg ctaaggtaat ccctgtttct gaagcatcaa atttagtgat cggtgcgatg
    20641 ccattcgtcc cctcttttaa gctattcaaa aagctgtctg cgtcatttcc aataggtgaa
    20701 gtgactccat agccagtaat taccactcga ttcatttgtt tgctctcctt ttcttatccg
    20761 tgcatgacca atccgccatc gacattgatg acttgtccgg tgatatatgg attttgtgct
    20821 aagaaaactg ctgtttgtgc aatatcttct acttgtccga aacgctgcat cggaatttgc
    20881 ttttccgctt gttccttgac tttgtctgct aaaacttccg tcatatcagt tgtgatgaat
    20941 cctggcgcaa tggcattaca tgtaatgcct ctagttgcag cttctcgtgc cacagatttt
    21001 gttaatccta ctacgccagc tttactagct gcataatttg cttgaccaat atttccaatc
    21061 aacccagata cactagagag attaatgatg gccccttctc gctgcttcat catttttttc
    21121 aatacgtgct gcgtcatatt gaaggttcca atcaaattga tatccagaca ttttttgaaa
    21181 tcttctgcat ccattcgcat cacaagtttg tcatttgtga ttccggcatt gtttactaac
    21241 acatggatcg atccaagctt ttcttctgct tctttgatca tttgtcccgc tttctcataa
    21301 tcagagatgt caccggaaac tcctacgcat tttacaccga atgcttcgat ttcttctatc
    21361 ttttcttttg gaatctctcc gcgaccattc aaaatgatat ttgctccagc ttttgcaaaa
    21421 gctaaggcca tcgctttgcc gattcctctt gtactacctg tgataaatac attttttcct
    21481 tttacatcca taacgttcct ccttatagta ttgctattgt ttcatcaaat gtctgcttat
    21541 tctcaacacg cagaactggt attgttttat cgattttttt cataaatccg cttaatactt
    21601 ttccggggcc gacttcgatg acttgttcga tgccgattgt ttttaactta tggatactct
    21661 catagaaacg aacaggttgc atgacttgct gttcaagtag tcccgcgatt gtttctttct
    21721 tcattatttc cgttgttgtg ttgcttatga taggaaaaga aggttcagag aattgaatct
    21781 gacttaggtc cttcgccagt ttttttgctg ccggttctaa aatagctgta tgaaaaggcc
    21841 cgctgacatt tagagggatc attcttttga accctttttc ttttaatagt aaaacagctt
    21901 cgtctactgc tttttcttcg ccaccaatca cgatctgctg aggtgtatta taatttgcag
    21961 gagatacgat gccgtacttg ctagcttcat gacagctttc ctcgatcgtt tcaatcggcg
    22021 cattcatcac tgcaaccatt ttcccgcttc cagctggggc agcttctgtc atataagcgc
    22081 ctctttttgc tactaacgca actgcttcag tgaaggatag cgcattacta gctactaatg
    22141 ccgaatattc tcctaaactc agtcctaaag ctgcatctgg gatgattcca tgctccttca
    22201 ataagcgata atacgcgatg ctgactgtta gaatagctgg ctgtgtatat tgcgtctgat
    22261 ccaaacgttc attttctgta aagcaaagtt cagccatatc gtagccgaga atctcacttg
    22321 cttcatcaaa tgtctgcttt acaacggctt cttcatataa atcttttccc ataccttgat
    22381 attgtgcacc ttggccacta aataagaatg ctgttttcat gtttttctcc aatatatcca
    22441 gaatatatgt ttgattcttc ctattcaaaa aggccgagac attcctattt caggaaatgt
    22501 cccagccccc aacctaattt taatgattat gcagtttgtt tttctacgta agtcactaaa
    22561 tcttgaaccg ttttgattcc gtcttctgtt tcgattttca cgtcaaattc atcttcaatt
    22621 tcattgatga tttggaataa atccaagctg tcagcttcta agtcttcttg gatgtcagta
    22681 gtcaatttga tttcttcagt ttcttttcct aattcttctg cgatgatttc ttgaattttt
    22741 tcgaatacca taataatata ttcctccgat attgtttgct ttttattttt ataatttaac
    22801 agtctacaac tccatgagaa tacttcccca tgttaaacct ccgccatagc ctgtaaatat
    22861 tactttctgc tgttttccta aggtgatctt tccactagaa actgcttcat ccaataagat
    22921 agggatgctt gcagcagatg tattcccata tttatccata ttggtcagga acttttcttg
    22981 cggcatcttt acttttcggg caattttctc aataattctg aggtttgcct gatgcaaaag
    23041 cagataatca actggtttgt ctttcatgac ttcttggata ttgtttgcaa cgtctcgtac
    23101 ggcaaaatca aaaatatctc tgccagtcat ttgcaaataa tacgaacctt ctgaatcttc
    23161 tgaatagaac ggatttttat tttgctggta gccagcggtc aatgagctac ttctggtacc
    23221 gtcagctgct aatttttctt tcagcaattt tttctgtgga ttcgcttcta agagaatccc
    23281 acctgctcca tcgccaaaca acacagcagt cgtacgatca gaccaatcga ttagcttaga
    23341 tagtgtttca ccaccaatca ccagtccctt agttgagccg cagcggatca ttttttcagc
    23401 catgcttaac gcataaacaa atcctgagca cgcagcactg atatcaaaag cgatagcatg
    23461 agttgctccc aatttccctt gtacttgtgc tgctacagat ggtgtcgtgt aatcgggcga
    23521 cattgttgct actaaaataa aatccaaatc tttagggttt agatttttct gttttatcaa
    23581 tttttccgcc actaaaatgc ataaatcaga agtctcctga tttgttacac atcttcgttc
    23641 acgaataccc gtccttgacg aaatccattc atcagaggta tccatcatct tggaaagatc
    23701 attattagta ataatctttt caggaaggac gctggctgtt gcagtaatca ttccaaattg
    23761 ttccattaaa tatcctcact tatagtcttg caagaagtcg tgcaagtttt tcagtgcatc
    23821 cagcaaggca tgctgttcct ccgtagccat tccgtctaag atgttcttga ccatttcacg
    23881 atgaaaatgc tgatggaccc gaaatagtaa ctttcctttt ttcgttagtc ccaactttac
    23941 aacgcggcgg tcatcttcac tgcggatacg ttcgacatag ccttttttta ctaacttgtt
    24001 aatagcagtg gtcaaggtcc caacagtaat tgatagttct ttggctactt ctgaagttgt
    24061 ttttttcttg tacatcccga ttgcctcgat cgtgtgcatc tctgtgattg acagatcttt
    24121 gaattgagat tttttcagct cagactcttc gattgtcaaa atatcattga agacactaac
    24181 aagataatca tttactgttt ttaaatctgg ttccatgttc caccgtccac taattagttt
    24241 gattatcaaa tcttttgatg ttcaaactat atcttaactt ttttc
//
LOCUS       DAZULY010000013        23076 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000013 DAZULY010000000
VERSION     DAZULY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.013"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..543)
                     /locus_tag="ACG81A_000379"
     CDS             complement(<1..543)
                     /locus_tag="ACG81A_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5013777.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLK"
     gene            complement(620..1150)
                     /locus_tag="ACG81A_000380"
     CDS             complement(620..1150)
                     /locus_tag="ACG81A_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFS5013778.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1154..2014)
                     /gene="rlmB"
                     /locus_tag="ACG81A_000381"
     CDS             complement(1154..2014)
                     /gene="rlmB"
                     /locus_tag="ACG81A_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFS5013779.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2007..2402)
                     /locus_tag="ACG81A_000382"
     CDS             complement(2007..2402)
                     /locus_tag="ACG81A_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFS5013780.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2399..3811)
                     /gene="cysS"
                     /locus_tag="ACG81A_000383"
     CDS             complement(2399..3811)
                     /gene="cysS"
                     /locus_tag="ACG81A_000383"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFS5013781.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3808..4344)
                     /gene="epsC"
                     /locus_tag="ACG81A_000384"
     CDS             complement(3808..4344)
                     /gene="epsC"
                     /locus_tag="ACG81A_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFS5013782.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4430..4669)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4666..6126)
                     /gene="gltX"
                     /locus_tag="ACG81A_000385"
     CDS             complement(4666..6126)
                     /gene="gltX"
                     /locus_tag="ACG81A_000385"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFS5013783.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6296..7453)
                     /locus_tag="ACG81A_000386"
     CDS             complement(6296..7453)
                     /locus_tag="ACG81A_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFS5013784.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7487..8857)
                     /gene="radA"
                     /locus_tag="ACG81A_000387"
     CDS             complement(7487..8857)
                     /gene="radA"
                     /locus_tag="ACG81A_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFS5013785.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8920..9447)
                     /locus_tag="ACG81A_000388"
     CDS             complement(8920..9447)
                     /locus_tag="ACG81A_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFS5013786.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9677..10483
                     /gene="proB"
                     /locus_tag="ACG81A_000389"
     CDS             9677..10483
                     /gene="proB"
                     /locus_tag="ACG81A_000389"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFS5013787.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10480..11727
                     /locus_tag="ACG81A_000390"
     CDS             10480..11727
                     /locus_tag="ACG81A_000390"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFS5013788.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11803..12147
                     /locus_tag="ACG81A_000391"
     CDS             11803..12147
                     /locus_tag="ACG81A_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013789.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12264..12590)
                     /locus_tag="ACG81A_000392"
     CDS             complement(12264..12590)
                     /locus_tag="ACG81A_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013790.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13205..14407)
                     /locus_tag="ACG81A_000393"
     CDS             complement(13205..14407)
                     /locus_tag="ACG81A_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5013791.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14524..15039
                     /locus_tag="ACG81A_000394"
     CDS             14524..15039
                     /locus_tag="ACG81A_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5013792.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15238..15684)
                     /locus_tag="ACG81A_000395"
     CDS             complement(15238..15684)
                     /locus_tag="ACG81A_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS5013793.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15731..17209)
                     /gene="msr(C)"
                     /locus_tag="ACG81A_000396"
     CDS             complement(15731..17209)
                     /gene="msr(C)"
                     /locus_tag="ACG81A_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS5013794.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17563..18192)
                     /locus_tag="ACG81A_000397"
     CDS             complement(17563..18192)
                     /locus_tag="ACG81A_000397"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFS5013795.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18176..18640)
                     /locus_tag="ACG81A_000398"
     CDS             complement(18176..18640)
                     /locus_tag="ACG81A_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5013796.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19127..20395)
                     /locus_tag="ACG81A_000399"
     CDS             complement(19127..20395)
                     /locus_tag="ACG81A_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS5013797.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20626..21363)
                     /locus_tag="ACG81A_000400"
     CDS             complement(20626..21363)
                     /locus_tag="ACG81A_000400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5013798.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21430..21999)
                     /locus_tag="ACG81A_000401"
     CDS             complement(21430..21999)
                     /locus_tag="ACG81A_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFS5013799.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22127..22999
                     /locus_tag="ACG81A_000402"
     CDS             22127..22999
                     /locus_tag="ACG81A_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5013800.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
BASE COUNT         6479 a         4935 c         4191 g         7471 t
ORIGIN      
        1 tttgagtttt actctcccgt aagcgctcat tacttgcttg tgatccattg ctaaacattc
       61 agccatgtct acaaaatcaa cgccttgcat ataataatga aaaattttag cttctatttt
      121 tgaaaactgg atttcgcaat tttcgattgc ttcttttaat aaaagatgct gaattgctaa
      181 aggttcttgc aatgtgatct ctgctaaaaa gtctggtcct tctgtctcta ttttttggtc
      241 gattgagact tcatcttttt gatcacgacg cttcagcgcg ttctgcttcc tcaacagaga
      301 atagatttga ttgcttataa cgcttttata ataacttcca aagctaacac ctttgctttt
      361 ttcgtaagtt ctcaatgcct gatataaagc gatccttgct tcttgttccc aatcctcaga
      421 aacataattt cttaaataat aggtgttttt caaacgtagt aaaagtggaa aatatttttg
      481 atacagggca gcaaatgctt cttcgtcacc attacgtgca gcgcgcaata actcctcaga
      541 cataaaataa ttcctccata aattcgttta tggaggaatc ttatcatcaa tgattcatcg
      601 aactgattag aacgatatgt tatttatttt ttctcattaa atcgtccaat ttttgggaca
      661 atttttttaa ctgctcttca ttccatggag aattacgtcg gaaatcaata aaatgcatat
      721 ctttattttc ttgggctatt gttttttccg ttttttttat cgtcttatat aattcataag
      781 cagaagttcg taatgctcct tgggaaaaga tcatccactg ttctgctaaa tcgcttgtcg
      841 ccactgtcac ttgtgtcaaa cggtcattca gctgaccagc gattctctcg atataactgt
      901 cagccgtttc gtcttctttc gtaaaaacaa catccaactg gtatttctta taattttgtt
      961 ggatgcccgg aactagttgg gcatcgaaga caacgatgac ttccattcct tcatatttcg
     1021 cataatttga taaacgatgt aaaagagctt ctcttgcatc cgccattttg tcctgctttt
     1081 taagctggac aagttccggc cacgaaccaa tcatgttgta gccgtctaca atcaacagct
     1141 gttttttcat ggtctactcc ccctgacgtt gggaaaaagc ctgatacata agcaaaccag
     1201 cagcaacact ggcatttaag ctctggacgt gcccggtcat cgggatcgtc agcaattcat
     1261 ccatttcttt tttcaatcct ggactcattc ctttgccttc attaccaatg actaaagcaa
     1321 ttgatccttt ggcattccat ttttggaagt ttgtgccggt catatctgtg ccaaataccc
     1381 aaaatcctgt ttctttgagc tgttgcacag cttgtaccaa attcgtcacg cgtgcaacag
     1441 ggacatgttc gactgcccca gtggaagtct ttgttacgat aggtgtgatt ccaactgctc
     1501 ggtgttttgg tatgatcaca ccatcgacgc cacttgcgtc tgctgtccgt aaaatcgacc
     1561 caaaattgtg tggatcttcg atgctatcca aaatcaaaaa gaaggggtct tcttttttct
     1621 cttttgttcg ttcaatcagt tggtcaaggg ataaatattc ataaggtgta atcgcaagaa
     1681 cgactccttg atgcacgccg tgatcgctca aagtttctaa tttttgttta ggaacccatt
     1741 tgactggcac agcttgttca cgtgcgattt ctttcagttc ttctactttt tctcctcgtg
     1801 aatcttcttg aagaaagagt ttattccctc gcccttgttg tagcgcttct actgcggcat
     1861 ggtgcccaaa gacgaaatta tcttctaatg cttcttcatt ttgatcagaa actgactgat
     1921 ttcgtccagt ttgcttttta tttgaatatc tattttgttt tttgtcttca aatccacgtt
     1981 tcgattgatg tttgtttgtt ctttctttac ccattttttt caccaacttt ctgaatacac
     2041 cagcggatca attcttccat ccgctcggtt tgttttgtca aatgcagata gcccatgagc
     2101 gcttcgaacc ctgttgcgac ccgatacgtc gtaacatcag cattttttgc gctcgtatgg
     2161 cttttagcat ttcttcctct gcgataataa agttcttctt cttctgtcaa aagatgttct
     2221 tccagcattt gctgaatcaa aaaggcttgt gcattcgcag aaacataatt cgttgccgca
     2281 cgatgcagct tattgggttt tgtttctcct tgatcaatca ggtggtcacg aacatagatt
     2341 tcgtaaatcg catctccaac ataagccaag gctaaaccgt tcaattgtgt gtaatctctc
     2401 atgcttctct tctccatcgg gttccttgcg gtgtatcttc taataagatc cctttttctt
     2461 tcaacaaatc acgaatttcg tcgcttcgga caaaatcacg attgcttctt gcttgatttc
     2521 gttcttcgat caattgttca atctcttcat ctaataactc ttctgctgta aataaaatac
     2581 cgaagatcga aagccattgc gagaataaag cttctccttg tgccaagaca gcggcagaaa
     2641 ctttcggctg gtctagataa gtgttcagcc acttagcaag ctcatagacc actgtgatcc
     2701 catttgctgc attaaaatca tcgtccattt cttcgatgaa tcgctgttct aaattttcta
     2761 acaaagcgat tttatccgca tcatctgcca aattctctga ggcagtttct tggcggaatt
     2821 tcaaattgtc aaacgtattc ttcagtcgtt gtaaattggt ggctgcttct ttcatcgtcg
     2881 tttcactgta gcggatcgga cgacggtatt gtgtggtcgc catgaagaaa cgcaagactt
     2941 gtggatcaac gtttttgatc atttcatgta cagtgatgaa attccctaga gatttactca
     3001 ttttttcatc gtcttcgcca atcgttacat agccattgtg catccagtaa ttagcgaatt
     3061 ttttccctgt tttcgcttca ctttgtgcga tttcattttc atgatgagga aattctaaat
     3121 cttgcccgcc accatggata tcgatcgttt ctcctaaatg ttttgttgcc ataacggaac
     3181 attcaatatg ccaaccggga cgtcctttgc cccatggtga atcccaagaa atctcgccag
     3241 gttttgcttg tttccataaa gcgaaatcta atgggtcttc tttttgctgc tgttcttccc
     3301 ctgtacgctg acttgctccg acttccagtt catcgatgga ttggtcactt aatttcccat
     3361 aattttcaaa tttacgggta cggtaataca catcacctga agattcataa gcatagcctt
     3421 tttcaaccaa cacttgaata aatgctaaaa tatctggcat atgatccatg actcttggat
     3481 gcagtgtagc aggttcgaca ttcaacgctt tcgtatcttc ttcaaatgct cggatgaaac
     3541 gatcagcaac ttctggtgct gtaataccta attcgtttgc tgtacggatg attttatcgt
     3601 ctacatccgt gaaattcgat acgaaatcga ctttatagcc tcgatattca aaatagcgtc
     3661 gaattgtatc gaaagcaatg gtgctacgag catttccgat atggatataa ttatacacag
     3721 tcggtccgca gacatacata ccgacatgtc ccttttgtat agggatgaat tcttcttttt
     3781 ttctggtcaa tgtattgtag atttttatca ttctgatctc ctcacttttt ctcctttgat
     3841 tcgaacgact tttgctggta ctcctaccgc tgtagcgtcg tctggaacat ctttcaacac
     3901 aacggcacct gcaccgattt tggcgttttt tccgatcgtt accggtccta atatctgcgc
     3961 attcgcagag agcatggctc cttgtttaac agttgggtgt cttttacccg tttcttttcc
     4021 tgtgccgcct aatgttacgc catggaataa aatcacatcg tcctcgattt ctgcggtttc
     4081 gccaatgacg attcccattc catggtcgat gaacacgcca gttccaatcg ttgctcctgg
     4141 atggatctca acaccagtta ggaaacgcca gaattgagca ttgatcttcg ccaataaaaa
     4201 gagccggtgc tgatataaaa aatgagagaa acgatgccaa aacaaggcat gaaaaccagg
     4261 gtatgtcaaa acgatctcag ctgtcgaacg tgcagccgga tcgtttctct ttgctgcatc
     4321 aatcgctcgg gtaaaccaac tcatgacttt ccactccttt cgtaagaatt ttatgtagga
     4381 aatcatcttc ttataaagaa aagaagcgcc ttttaaataa gtttccttat caaaagacgc
     4441 tttccgcgtg gttccacttt tcttcataat caatgattat ctctgacagc taacgcctgt
     4501 tatacgttct tggctacttt gtttcctata aaacagtttt tcctgttttc tgtgtttcgc
     4561 caagaccact cccagaggca tttcggcaac cattcattga taccttgcac cacccggtat
     4621 ctctcttgta atgatcagct gcttactttt ttctgttcat cgtgtttagt tcagtacttg
     4681 atttaaatgt gctaatgatt tttcacgacc aagcaattcg atcgtttcag gcaattctgg
     4741 accatgcatt tgtcctgata cagctacacg gattggcatg aaaagatttt ttccttttac
     4801 gccagtttct ttttgaacag ctttgatggc agctttgaca gttggagcat ctactacttc
     4861 catttgttct agctgtgctt tgaatgcgtg gagaacagta ggaactgttt caccagcaag
     4921 tacttctttt gctgcttcat tcaatacagg atgttcattg aaaaatagcg tagacagatc
     4981 aacgatttct gcagcatagc tcatttgagg ttgatacagg ctaacgatct tcttcaccca
     5041 ttcgattgtc ttttcatctg ggttttctgc tacacgtcca tcagcgatta gataaggcag
     5101 acacatgtca gtaaatgcat ctagatccat tgctttgatg taatggttgt ttacccattg
     5161 cagttttttc gcatcgaaag ctgcgggtga tttacttagg cgttttggat cgaacatttt
     5221 gatcaattct tggtgttcga agatttcgtc ttcgcccaca ggtgaccagc ccaataatgc
     5281 aataaagttg aacattgcat ctggtagata gcctaattcg cgatattgtt cgataaattg
     5341 caagattgat tcgtcacgtt tgcttagttt tttacctgtt tcactgttga tgatcaatgt
     5401 catatgtccg aaggttggtg gtgtccagcc aaatgcttca tagatcatca gttgttttgg
     5461 tgtattggca atatggtcat ctccacgtaa aacgtgagtg attttcatca aatgatcatc
     5521 gaccgcaaca gcaaaattat aagttggcat accatcgcgt ttttggatga caaagtcgcc
     5581 accgatatta tcggattcga acgaaatagc gcctttcacc atatctgtaa atgcgtattc
     5641 tgtattgcga ggcacacgga aacgtacaac agattctaga ccttgtgctt ctttttctgc
     5701 ttgttcttca ggtgtcaagt tcgcacatgt tcctgcgtaa tgaggcattt ctcctcttgc
     5761 gcgttgtgct tcccgttctg cttctaattc ttcttctgtg caatagcatt tgtatgctcg
     5821 attgctagca agcagctgat caatcagcgg ttgatagatt tctttacgtt cagattgacg
     5881 gtaagggccg tattctccag gattttcagg agattcatcc caattcattc ccaaccaagc
     5941 aaggttttcc aactggcttt tttcgccatc ttcgatatta cgtttttggt cagtatcctc
     6001 aatacgaata ataaagtctc catcattgtg gcgggcaaac aaataattga ataatgcagt
     6061 tcttgcattt ccaatatgca ggtgcccagt tggacttggt gcatagcgta cgcgtacttt
     6121 cgtcattttc ttcctcttct ttcaaactag actttgacta acagcaaaaa ttccgtcagt
     6181 tacggcttct caaatatcat ttatttaaag ccactcagca caaattttac cttttctaag
     6241 gtaaatagta aagccaactg gttgaatttc cttaccagtt ggctgaattt cttttttatt
     6301 ttgattgttt agtctttttc tctgtctctt ttttattgtc gattcctcgt cctgcatgtg
     6361 caggtttagc aaagatcatg cgtccagcag ctgtttgaag ggcacttgtt acaacgactt
     6421 cgatacgttc attcatgtaa tgctgtccat cttctacgac aaccatcgtg ccgtcatcaa
     6481 ggtaggctac tccttgttgg cgttctgtcc cggcttttac tactagaaca ttcatcgttt
     6541 caccagggat gacgactggt tttacggcat ttgccaacgc attgatatta agaacaggta
     6601 cattttggaa ttcagagact ttattcaagt tatagtcatt ggttacaatg acaccatcta
     6661 aaagcttagc caatttgatc aatttgctgt ctacttcact gatatcttca aagtctccat
     6721 cgtacatttc gacagatata ccctcttctt tttgcaaggc atttaggata tctaatccgc
     6781 gacgaccacg cacacgtttc aaactatccc ctgagtccgc gatgtattgt agttcgtaaa
     6841 gaacaaaatt agggatcatc aaggttcctt ctaagaatcc agtctttgta atatcataaa
     6901 tccgaccgtc aatgatcacg cttgtatcca agattttata tttatggaaa ttttctcctg
     6961 attttcgttc taacagttga ctctcaactt gactagctgt ttcttgctcg atttttttgc
     7021 ttcgtgaacc aaaaactttc ttccattctt cgattcgtgt cgttcccatt ctcaatccca
     7081 gatagcctaa gatgatcatc agtaaaatcg gcaatacact attgacgaat ggaatttcca
     7141 tattgtaaag aggggtcgag atcacaacac ctaaagtcaa tccgatgatc gcaccgatcg
     7201 caccgaagag aagataagtc aagctgacct cacttagttt ttgttctact tgtttgataa
     7261 tagctgagat atgtttggcc aacagcaaag ataataaaaa gaaaataagt gcgcctaaca
     7321 ggctgttggt aaatgtatta ttcagccatc cgtttgactg ttgaccgatt gcctgccaag
     7381 ctgagggtaa aaatgaaata cccaagcttg cgccgacaat gatcatcagc aaagtaatga
     7441 cacgtttttg catcgttttt cctccgtttc gacaatcatt tattagttag cggaacacac
     7501 gcttcaacgt ttcagccaaa gttgctactc caactatctc gattcctttt ggcgcttccc
     7561 agttacctaa gttgttttta ggtaggtata cttttgtgaa gccgagtttt tggacttctt
     7621 tgactcgttg ttcaatatga ctgacacgtc tgatttctcc agtcagtcca atttcaccga
     7681 tgaaacattc cgttggctgt gttccttttt ctttatagct tgaagcaata ctgacagcga
     7741 tagctaaatc aatcgctggt tcatttaatt ttaccccacc tgcagctttt aagtaagcat
     7801 cttgattctg aagaagcaac ccagctcttt tttccagcac tgccatgatc agagataccc
     7861 gattgaaatc aagacctgtt gttgttcgtt tggcatttcc gaacatagta ggtgtgacta
     7921 atgcttggat ctctaccaaa attggacgtg tcccttccat tgccacaacg atagcagagc
     7981 cagttgcacc gtctaggcgt tcttctaaga aaatctgtga aggattagcg acttcttcca
     8041 atccatgctc atgcatttca aaaatcccaa tctcattcgt tgagccaaat cggtttttta
     8101 ctgctcgcaa aatccgaaaa ctatgatgtt gttcaccttc aaaatataac accgtatcca
     8161 ccatatgttc taacattctt gggccggcga tcgagccttc tttggtaaca tgaccgacga
     8221 taaaaatggc tatcccattt gttttagcgg ttttgagtag ttctgcagtg gtttctctta
     8281 cttggctgac acttcctgcc acacttgtga tgtctggctg cgtcatcgtt tggatggaat
     8341 caatgataac ataatcaggg gagatatgtt cgatggcacg actgatttcg ttcatatctg
     8401 tttcagcgta tagataaaat tctgtgttga tacttccgag gcgttccgcg cgtaatttta
     8461 tctgctctgc gctttcttcg ccggatacat ataagacttt tccgcctatg gctgctaatt
     8521 gctgagaaac ttgtaacagc aaagtggatt tcccgatacc tggatcgcca ccaatcaaga
     8581 ccatagaccc tggcaccaca cctccgccta acacacggtt cagttcttct aattttgttt
     8641 tgatccgagg ttctttttta gggacgacgt ctgcgatttt tgttggtttt gctttttctc
     8701 ctgtcaaact tgtccgagtt ctgcggtccg ttgtatcttg ttcgatttct tcgaccatcg
     8761 tattccattt tccgcaattt gggcagcgtc ccaagaactt aggcgacaca tacccgcaat
     8821 tttggcaaac aaattgtact tttgattttt ttgccattgt accgcctcct tttatcctaa
     8881 tcatgagaca ttaaatcaag cttaagtatc tttaacgttt tatttactgg aagaaccaaa
     8941 tccgccttga cgttgagcag tcgtttgatc ttgatctgct tttaaaaacg gcaaaaagat
     9001 cccttggccg atacgctcgc cttttttgat cactacatcc ttgaaaccaa agttggtgaa
     9061 ttggaacatg atatggcctt catttgcctc attattgtaa taatcactgt caatcacacc
     9121 tactccgttt gtcaacacta aaaaccgctt caacggattg cttgaacggt tagcaagctg
     9181 caaaaattca tcttctccca tgtaagcttt gattccagta ggcacgaggg ttggtttcaa
     9241 ttgtttttca ttttctgata catctttttt caatccatct gcgactgttt ccaacccctt
     9301 tttccagcct gctttccaga tacttggaat cacgacctct tctgctgctt caaaatcata
     9361 gccagcagca tgatgtgtag cgcgctgcgg tagattgata ttttgatctt tatatttaga
     9421 aataacttca aatcctcttt ttctcaccgg ttttcctccc taaataaact actcctattc
     9481 taacatatcc gaggctgtaa aagactttct ttacaaattc ttcaagaaat tctgctatct
     9541 taaagtaaac cattaattcg ttctttttcc aaacgactgc aagttgtaca aaaaaactat
     9601 tttttacatg actttactcg tcaaaacaag tagaatagaa cgaaattaat gagtggatat
     9661 ttgattgagg tgtcacatgc gtaaagaaat taaaaatgca aaacgaatta ttgtcaaagt
     9721 aggaacaagt acattgattt atcccaatgg aaatatcaat cttgcggcaa tcgatcagtt
     9781 agcttttgtc ctttctgatc ttagaaatcg gggaaaggaa gtgatcttag tctcatcagg
     9841 agctgtaggc gttggtatgc ataaattaca tttgtcagaa cgtccgactt tgattcctga
     9901 acagcaagct gcggcggctg ttggtcaagc cgaactgatg aatatctata atcaaagatt
     9961 cagtgtatat aatcaacaga cggcacagat cctcgtgaca agagacgtgg tagattatcc
    10021 tgaaagtcgt aaaaatgtga ggaatacatt ggaacagctt ttgattatgg acacgatccc
    10081 tatcgtgaac gaaaacgata ctgtagcagt ggatgaatta gatcatctga caaaatttgg
    10141 cgataatgat cagctgtcag ccatcatggc aaaacttgtc gcggcagacc ttttgatcgt
    10201 cctttctgat atcgacgggt tttattcaga taatccgact acaaatcccg aggctcagat
    10261 gtatcgctat atcgataaaa tcgatgaaaa gattctggca caagcaggtg gtgctggtag
    10321 cacatttgga acaggcggta tgcagagtaa attaaaagcc gcttctagga tatttgaagc
    10381 aaacagcgca atgattttag caaatggaaa aaatccggca attatttttg atattcttgc
    10441 aggaaaagaa gtaggtactt tatttaagga ggacttgaaa tgactgactt gatgcaatta
    10501 ggacaacagg caaaagatag cgcgcaacaa ctagcactta tgcctacaaa ccgcaaaaat
    10561 gagctacttg ttcaaatggc acaaaccatc aaaaagaatc aacaagcgat cattgaagca
    10621 aatcataaag acttagaaaa agcagtcgaa aacaatatca gtgaaacgat gcaagaccgc
    10681 ttgcagctga cacctgagcg aatcaatgcc atggctacag agatcgaaaa aattgcttcg
    10741 ttagacgatc ctattggaaa agtagatgag atgtggacga acaccgacgg attgagaatc
    10801 ggcaaaaagc gcgtgccact aggcgtgatc ggcatcattt acgaatctcg tccaaatgtc
    10861 acgacagatg cagctagtct agcattcaaa tcagggaatg ccgttatcct tcgcggtggc
    10921 aaagaagcat tcttctcgaa tcaattatta gtccaattat tgcagcaagt actggtcagt
    10981 gctggcgaat ctccttatgc catccagttt gtcgatgata cttctcatga aactgcagga
    11041 cagttgatgc gattgacaga atacttagat gtgctgattc ctcgaggcgg agcaaaatta
    11101 atccaacgag tcaaagaaca ggcaacggta cctatcattg aaacaggtac aggaaataat
    11161 cacgtatata tcgataaaga agcacagtta tctatggcag tgaacatcat cgtcaatgca
    11221 aaagcttccc gtccatctgt ctgtaacgcg gcagaaacat tgctgatcca ttctgagatc
    11281 gcaccttttt tcttgccagc tattgaaaaa gaactggtag aacacggggt ctctttacgt
    11341 gcggatgctc gtgcgttaga atatctagag acggctgctt tagcagaaga cacagattgg
    11401 gatactgaat atctagatta tatccttgct gtcaaagtgg tcgattcttt ggatgaagca
    11461 attgcacata tcaatcggca taatactaaa cattcagaaa cgatcgtgac agatagttat
    11521 gcggctagtc aacgtttctt aaatgaagtg gacgcggcgg ctgtctatgt caatgcctcc
    11581 actcgtttta cagacggatc ggtatttggt tttggtgcag agattggtat ctctactcaa
    11641 aagttgcatg cccgtggtcc aatggggtta aatgaattaa cttccacaaa atatatcatt
    11701 tatggcgatg gacaaatccg cgaataatgt ttcgtgtgaa acattattcc cgccttttgc
    11761 cttttttctg taaaggttct atccaactag aaaggtgtca atatgtttga aattttaatg
    11821 atttttttta tttaccttat ttctctgaat atcgctgctt ttctgggagt cggtatttta
    11881 tcccttttct ttcaattcaa aaaacgaagc atagggagtc aacgggaaaa atggtcgcag
    11941 tattttgaca aaatcgggca aaaagggctg gttacacgct tgcacattag ttacatggtt
    12001 gcattatgct tattagcgac aatcaattac tattcttttt tcgatcattc aatcgcttat
    12061 acgattacgc tactgattgc tgggattttt cacttatctt ataaatatca gctgaataaa
    12121 aaccacttga atcggacttt ccgctaaaaa atatccgtac gataagtaga gtgccttgaa
    12181 gatcggcaaa aaactgccgg actttttaga ggctgtgaca aagattttgt cacagcctct
    12241 ttttccatat aaacggcgtt cgcctaccag ctgcctggta aatctgtttc ctcttcgtta
    12301 agaagcgaaa atccgtcaaa tgtttcgcca tcataataac gaagcagctc atccatggac
    12361 aagttcatcg ttttttcttt ttcttcttcc gctgattcag ccagtagctg ttcccgatac
    12421 acctgataca taccagctat tttttgctgc atcgcttttc tagtttcacc ttccgcattg
    12481 attattggca attctttgat atagccacga taaatcaacc gatcatctga aaattcaatg
    12541 ggtaattcaa cgagtgtcaa cccgacttcc tgaaaatatt cttccagcat ctattatccc
    12601 cccattcttt ttcttaaatg aattttagct tacctactga aaattttcca gtttcattat
    12661 tttgagaaaa aaagggaatg tgacaaaaat cgtgtcacat cccctttttc cgaataaatg
    12721 gtgttcttct gccaactcct caatcataag tcacaatatt caacaaacat gagaaactgt
    12781 tttttgaaat tgttccttgt gattcagagt taaacgacag tctcacaacc tcttggtaaa
    12841 cggcgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggtaaaata tgagaagcta
    12901 tttttccgtt ttgtttctct aatacttagg ataaaacgct gattttacaa cctctgttaa
    12961 acggtgttca atcagctgtc cttcggtatt agctcaaaaa acggcaaaat atgaggaact
    13021 atttttccgt ttgtttctct aatactcagg acaaaacgct gattttacaa cctcctgata
    13081 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagaa
    13141 gctatttttg tttggctgtt cttatgactt gatgcaggac agctttttca caacctctga
    13201 tttcttattc ttcttcattt gtagcaaaat aattacggtg ataaatgata caacatacac
    13261 tgataagaac aatggcacct gcaaaataaa acgcatagct ttcaccaata tgttcaccaa
    13321 tcacacctgc aataaatggt gctaaagctc caccgaaaaa gcggacaaaa ctatagctgg
    13381 acgaagcaac gtttcgttgc agacctggta tttccatagc aatcgtcgtc aacagtgtat
    13441 tgataattcc ttggaaaaat cctgctaaca caatgctgat cggtacaagg actggcatag
    13501 aagttccagc tcctaataac agcaaacaaa taatgaataa gacaaatgct gctaaaattg
    13561 tccgataagt cgtaaacttc cgctcaagag ccggtgcaac gaaaatcgaa gcaatagcca
    13621 gaagcacacc ccagccaaaa aatacaaaac cgacttctaa ttcagaatag ccaatcaata
    13681 aaaatggcgc atacgcaagt aaagtaaaga atccgaaatt atataacaat gcgattagtc
    13741 caactgaacg aagtttatga ttgcttaacg ctttgacacc ttcccaaagg cctacttttc
    13801 tctttggttt ctcagtttca ggtaaaagta ccgctagggc tataaaaccg caaaacatca
    13861 aaaatgctac cccaaaaaat ggataacgcc atgaaccact gcccaagacc ccaccgacta
    13921 gagggccaac agccattccg cagccaagtg ctgcttcata catcatgatt gctttttctg
    13981 tgttaccaac taaaatactg acgatcgttg ctaaagcagt agagacaaat aaagcattcc
    14041 cgacacccca gccggcacgt aacccaatca gcattccgat attcccagat aatccgccaa
    14101 gaccagcgaa tacaacgatg ataaacaatc ctgtcatcaa tgtttttttc aaaccgatcc
    14161 gactagataa gaaacctgta aatagcatca ccacaccagt gactaacatg taactagtaa
    14221 ataacagggt cgtttcagct ggcgtagcgt ttaactgtgc agcaatcgtt tttaagattg
    14281 ggtctactag acccactccc ataaaagcga tcatacaggt agctgctaca actagcgctg
    14341 aacttttccc ctttgtttct tcatttgttt gttcttgtat atctatttga ttctccaata
    14401 tttccatccc tttctctcta ttagcatcat acatgattta ttttacatgt caatatgcac
    14461 atgtaaaacg tggaaacttt actgtgatat aataaagaaa ataagacgag aggtaggatt
    14521 tttatgaata ctttagccta tatattatta tgcgtgcttt tatccaaacc acgttctggg
    14581 tatgaattga agcagctgat cacaattttt tgggaagccc atcacagtca gatctataca
    14641 acgcttgcca agttagacca gcagggttat gtgaccgttt tggacaaaga cgagcactct
    14701 caaaaaaaga tctaccactt gactgatgaa ggacgtgctc ttgtagagga atggatcaaa
    14761 gaggaaactc ccgctcctac gcaaaaagat gaatttctag caaaaatcta tgctttttcg
    14821 acgctcgata aaacaacagc tactggactg ctattcacta gagaacaaca gctgaacaaa
    14881 atcatgcaaa aaaatcaaaa aaaattgaat gacctctcgc tagacaagaa acaagaattt
    14941 ggacgttatg ttgtgatcca gcgcagaatt atgctttgtc agcaggaact ccaatggtgt
    15001 cagcaagtaa gagaacaaat gcagcagttt tttgagtaag ggataaacag aacctttcta
    15061 gaaatatcta ttgaagagca aattatcttc aataaaagag caatacagtt ggaaaagaaa
    15121 aagcgatccg aacgacaggc caaaacctat cgttcggatc actatttttc tagaactgca
    15181 ttcaagagac tatgacaaaa agcatgccaa ggcctttttt caaataacag tgttccatca
    15241 ttcattactt tgaattgctt ttagcacact tcttgaaagc gggccaacga tgatcaactg
    15301 caacggtaaa gcggcaatca cattcatttt ccaagcattc atataagcag agaataattg
    15361 atcaggtatt ccttgttcca tgatcacacc gaataaagac atacatgtca ccattcctaa
    15421 aaccatcaag catgaaatag ttaagatcat ttggaccttg ctttctggat ttactggtag
    15481 tttaaaggca atttttttcg ccagcacacc gatgatgaag acatccaaaa tgaaagccac
    15541 cacgaatcca ggcactagac caatcaacaa acttgataaa tgaagatctt gatgcagcca
    15601 taaattatac gcgctcatcc ccaagaccat caaaaaacac atcattgttg taaaaattat
    15661 tccttctttt ttatttgtag gcaatctgta ccactccttt aaatttgtca cgaaaatcat
    15721 cgtagcacat ttaaaaattt ctcgtaagta ctttttttat tttctgccct tttatttcat
    15781 aaacttcatc agccgtacgc tcgacaaagt aagaatcatg agaagtaaac aaaatcgttc
    15841 ccggatatat ttgcattagc ttctctaaag cttcgatcgt tgccatatca ataaaattag
    15901 tcggttcatc taacagcaac acattacttg gcttagtaaa aagcaaagca atcgtcagac
    15961 gggtcgcttc tcccccactt aatgtacata acggtttcgt caaagtttcc tgagcaaacc
    16021 ctaagtgatt cagcaaacta cggacaagcg attctgaaaa ctctgtttgt ctcatgacaa
    16081 aacgaatgat ggattcttca gatgtcattt gataaccctt ttgctgatat acttgaaagc
    16141 ttactttcgg agagagaagg attccttctc cttgtttgcg gatatgttct aataaggttg
    16201 tcttacctga accattttca ccgacaatcg cgattttttt acctaaagaa aactgaaaat
    16261 caccatttac cagtaatgtt ctcccacttc tttccaattg gacattttgt cccatgatgg
    16321 gatagcggct gtggagttca agagattttg gtactggaaa acggatttgt ttcatctgct
    16381 gcggcttggt tgtttctcct attttttgga gtcttctctc taatgttttc gcattctttt
    16441 ggatggcttt ttgtacactg tctttttgtt tagaggaaga caaccgatcc agtctgattt
    16501 gttgcttttt ctttttactg gacacttttt ccaacttttc cgcttgtttt ttcttttctt
    16561 ggatcgattt ttccaatcgt tttttctctt tcatgatcga ctctgcttct cgtagctgag
    16621 tctttttctc caattccttt tgagtgagat aggcactata attccccgaa aactctcgga
    16681 tttctccgtc ttttacttcc cagattttcg atgctagctc atctagaaaa tagcgatcat
    16741 gactaacaaa aaccagcgta ccataataat aacgaagctc ttcaatcagt cgatcaatac
    16801 tttttctatc tagatgggtt gttggctcgt ctagaagcat cccttgttca taagaagaaa
    16861 gaagacgtat caactcgatc tttcgttctt cccctccgct ccatttccgc tctgaagttt
    16921 cttccagtct taactggaaa tgactggcta attccaaatt taggtccttt gcttcagcga
    16981 gataggttaa ttgaggcagg tagttgaagg tgatctctgt ttggattttc ccatgatcgg
    17041 gaaaaagttc accggctatt agtttgagca aagtcgattt accgacacca ttttctccta
    17101 tgatccctat acgatcttgc tgatagacac gaagagaatc aatagatagt tctagctggt
    17161 tgccaaagct gacttgtaga tttgttatat ttactgctaa attttccata tgttttacac
    17221 tcctatttca tgtaaaacaa caattatcga tactaaaaaa gagcagaaaa atctgtttgc
    17281 aaaaaaactg catcacatct tttcctactc tgaacgaaat aactatgact aaatcaccaa
    17341 atctgtcata gctttcacct attctactgt cgttcgaaaa aatggacata ctccatccat
    17401 gaaattgttg tttatacatg tcttttgaat ttatttagtt gttattcaaa agttcgaaac
    17461 gtaatttcat cgatgcagtc atttttccga cctccttttt tattaggtta ccacacacga
    17521 ttcaaaaaaa gcaatagcta ctgaatagta actgaaaacc ttttattcga acatagtgat
    17581 cttttctggt gctaaacgaa cagactcgta accttcttct accgcttttc ctattgataa
    17641 aataataact ggaacataac ggtctttatc caaaccaaat gcctctgcca actgatctgc
    17701 ttcgaatcca ccaattggat ttgtttcata gccatgatca cgcgcaacta acatgaattg
    17761 catagccgct aagctagaat cgactttgac tacatcgttc atttcttctc ttgaaaaact
    17821 cttgtaataa ggaatgatcg cagctaattg ttgatctctg acttcttttg gcatcttccc
    17881 tgattcgtaa gcttggttgt agatttcttc gccatattcg taacattgca tatcgccaaa
    17941 aatcatgacc attgcagaag aagtatcatt ttgtctcgta ttgaaacgga tcaaaggacg
    18001 caagatttct tttgcttcct cgctttcagc gaccacaaag cgccatggtt tcatattgac
    18061 agaagaaggt gcagtcgtcg cttctttgat catttccagc atttcttcgt gtgggatttt
    18121 gtatgtttca tcatatacgc gaacggatct tctaccagtc ataattgttg aaaaatcatt
    18181 tttttgttcc attattatta acctctttcg tttctgttgt tatattgcaa agttgggtat
    18241 tcaatttatc taaaagtgtt agaaattgtt tcatttctac atcgctgaaa gccggggata
    18301 gcacggaacc tgtatcttta tggtccattt cacaatgctt gattttcttc tgggcttttt
    18361 ctgttagcgc tacatacact tcccggttgt tctgaggatt tctctgacgc gaaacatagc
    18421 catttgcttc caataccttc agatgacgag taactgctcc ttgatcaatc tgcaactttt
    18481 cttgcaaact gctttgtagc aatggatttt cttctttcag tctcatcatg atctcgtatc
    18541 tagtaagact tataccgata ttttcttcaa agatctggac gattttttga tcggctaatt
    18601 tgatttgata taaaaggtga ctaatctctt ctaaagacat aatttcctcc tttctaataa
    18661 ttgactcgtc aacgattgac ttgtcaataa tagaataaaa aaagaaaaaa gtcaaagaat
    18721 cagctgtgta ataaaaaaaa cgctctcaat agaaaagtat agagaaaaga aattttttgt
    18781 agaatgaacg gaaaaaatga taaagaaagc acaacgctaa aaccaagatt attgattctt
    18841 atcaaactat agaagatagc tcttctgcag tagataattt tacaccgcca aaaatttccg
    18901 aaatcgcctt ttgcaagaat cccgtcgaat attagaaatt ggatcagtat gcttaaacag
    18961 taataaaaaa gttatgacaa gatgttgtca caaccttttt tgagtaaacg gtgttcaaaa
    19021 acttgcatct gcggtaataa gttcaaccaa acaaaaaaca tgaaaatctg tttttgtttg
    19081 gctgttctta ctacttgatg cagggcagct ttttcactac ctctttttaa taaccataaa
    19141 atcctattcc ttcatatctc cagcccatcg gtgttagcca atctctttcc ttagcactca
    19201 aggtatagtg atgggtacca gttttttcat ttggattaaa tagtcgataa attggcttgg
    19261 aaccaccaga ataaaaactt agaccttcat aacgccaacc gatttttgtt aaagagttga
    19321 tctcagattg tgccattgtg taatgatggt ctccactgta aggattgtat aaacggtaga
    19381 caggttgtcc gctgtttggt gcttgccaag caatcccttc atattcccag cctacagttg
    19441 gcagataatt tttttctccg gcattcaatg tatataaatg ttctcctgta tgacggttat
    19501 acagacgata catcggagaa gttgaagttg gtgctgtatc atactgtgtc agattatagg
    19561 tttcgattac attgttcaat gtagaaccat aattaggcgc agtggcataa cgacctgtta
    19621 gccaagccgt agcatctttg taagagttcg tattgctttt ccatgcaccg gaatagtagt
    19681 aaacgcctgg ctgaaaagaa gtcgtcttca ataccgttac gttatcttgc aatgattctt
    19741 tgtacgaagg atattttcta aaagcggcat tcaccgtaat ccattgccca ttcacatatt
    19801 ccgaagtagg catcgtaaca gattggccat tatagcttcc tttaatccca aatagattgt
    19861 aattaggggc agaagccaaa gtactgttcc cccagccact ttcaacgatg gcttgagcaa
    19921 tcatgactga tgcatataag tcattggcac ttgccaaact ttgtgcagtt gaggcaatac
    19981 tatcaatgaa cgcttgttga gaattagcag atgagcgagc ataaaaagca tagccagtat
    20041 agttttcgaa ttcagacaaa tctgcttgat gggtatgtgt caatgtttgc ccatctgtca
    20101 attggttcgt gatttcttct gcattctctg gattgttctg agcttctgct gatgtactgt
    20161 cttcggtttg tgtttgttct gttgtttcag ttgttggttc ttcggttgaa attgtatttt
    20221 ctgagacagt agctgactgc aaagtacttt ctgtatcgtt ttcaattcct gtttctgaag
    20281 aactcgatgt ctgagttgcg acagtttctg tcgattcttc agctagcact gttgtgttgg
    20341 caactgctag atttcctaaa agaataacgc taaataataa attctttgct ttcatctttt
    20401 ttctcctttg aattatgaaa cttttcctaa ataacagtgt aactaaaaaa gtaaaagcaa
    20461 gtcaatgtcc atatttaaat aacatttgtt tctttttaat aaatttgctt tttctgcata
    20521 aaaagacgta aatgaagaat attcttctcc attcacgcct taaattgatg gtcgtttgaa
    20581 taactattta acaagtaatt ctctctggat attatccgtt atggattata cttcctgtgt
    20641 cgtcggacgg ataatcatgt tgctgacaga catccggtct ggcgtttcta tcgcaaatgc
    20701 tacagcagaa gctatatctt cagatgttaa tccccattct ttttgcgctt catgaagttc
    20761 aagagcagcc tttttgtcgc tgatggtttg atataattca gtttgaacag ctcctggaga
    20821 gatgatcgta gatttgatgt tattttgccg ttgctcttga cgtaatcctt ccatgattgc
    20881 acggacagca aattttgttc cgcaataaac ggcggaatca ggataaacaa catgtcccgc
    20941 cacagaatca gttgaaatga tatgcccaga tttttgtttt tccatgattg gaagtacggc
    21001 ggaaattcca tttaacacac ccatgatgtt gatatcaagc atattttgcc attcatcacg
    21061 gtgaccttca actaatggag cggttggcat gattcctgca ttattgtaca atacatcgat
    21121 tcgaccgaac ttttccatcg ctaaatcgat aactgcttgg acttgtgcaa aatctcttac
    21181 atctgctgtt tgaatatata gctcagcttc tggtaaagat tcttttattg cttttaggcg
    21241 atcttctcgg cgtgcagcta taactagttt agcccctttt tctgctaata gcttcgttgt
    21301 tgcttcaccg attccgcttg aagcgcccat gatgacaatc actttttctg ataaatcatt
    21361 cattctattc tcctcctgtt ttgaaaaatc tcttgcttat actcactatc tcttatttca
    21421 tcttactgtt caaataatcc gttcgacagg tttgacgatt ttggcaggga ttcctgcaac
    21481 gatggtattg tctgggacat ctttcgtaac agttgcattc gctgcaacaa tggcattctc
    21541 gccaatcgtt acgcctggca gaatagttgc tcctgctccg atccaagcat ttttcttgat
    21601 gtggattggc tttactgaca agcctcttcg ttcttttgga tcggttagat ggttcaccgt
    21661 gatgaggttg acacgaggac caatcaatac tgaatcttct aaacagatgc cacctaagtc
    21721 aacgaacatc acttgacgat tgataaagac atttttccct atactaatat gtttgccgaa
    21781 atctgtataa aaaggcagca gaacagtcac cgtatcatct actttgtcac taatgatatt
    21841 ttctagccgt ttgcggatat ctgctggttc atggtaccca gtattcagcg ctgctaaagg
    21901 ctcttcgttt tccgcttgga tctgatggat cacttcaaag agcggatccg ttactcgaat
    21961 ctctttattg acgatttgtt tttcgatgag gcttgtcatt ttgctccctc cttttatcct
    22021 tactttataa cggacggctt atgatgtcta atacttataa aacatactta actattcctt
    22081 ttaactatac ttagtggtat gcttttttaa aaacaggagg gaaaaaatgg atattcgtat
    22141 actgacgtat tttttagcag tagctagaga aaaaaatatc agccgagcag caaaatctct
    22201 gcatatcacg caacctactc tctcaaaaca actgaaagat ttagaagaag aacttggtgt
    22261 gacacttttc actcgaggct cccgagagat acagttgacg gattctggtc gatatcttta
    22321 ttcaaaagga aaagaaatct tgtcgttggt agaaaaaacg gcgaacaatt taacaaatga
    22381 cgcaatcatc tctggagaaa tctacattgg cggaggcgaa actaaagcaa tctctttcat
    22441 ttctgatagc cttcattcgt tgatcaaggc gcatcctgat atccagtttc atttatatag
    22501 cgggaatgca gatgatatca tagacaaact ggatagcgga ttacttgatt ttggactcgt
    22561 aatcgaaccg acaaacaaac aaaaatacca gtcatttcga ctgccagccc aagatcgttg
    22621 gggacttttg atacatcgga atcatccttt agcaaagcaa acagtcatcc atccgcagga
    22681 actgaaaact gttcctttga tcatttcccg gcaaacgtca gtggacagtc aactagctga
    22741 atggctaggt ttcagcatag atcagttatc gatcgtcgga acatataact tattgtacaa
    22801 tgcttctttg atggtcgata acgggacttt tggtgcatta gctctagatg ggatcatcaa
    22861 tacttatggg acggaaaaag tattcatccc actcgatccg ccattacaag cttcactgaa
    22921 tctcatttgg aaaaaagacc agcctctttc tcctgctgct cgtaaatttt tagatgtcct
    22981 aacggagaaa tacagttaag tagagttttt ttccttcgct ttcagctttt taacaacgaa
    23041 taagttaagc aaaagatcca atgattaaat aacata
//
LOCUS       DAZULY010000014        22662 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000014 DAZULY010000000
VERSION     DAZULY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.014"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            126..1622
                     /gene="glpK"
                     /locus_tag="ACG81A_000403"
     CDS             126..1622
                     /gene="glpK"
                     /locus_tag="ACG81A_000403"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFS5013801.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            1636..3459
                     /gene="glpO"
                     /locus_tag="ACG81A_000404"
     CDS             1636..3459
                     /gene="glpO"
                     /locus_tag="ACG81A_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFS5013802.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            3460..4176
                     /locus_tag="ACG81A_000405"
     CDS             3460..4176
                     /locus_tag="ACG81A_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS5013803.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            4482..6410
                     /locus_tag="ACG81A_000406"
     CDS             4482..6410
                     /locus_tag="ACG81A_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFS5013804.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            6536..7090
                     /locus_tag="ACG81A_000407"
     CDS             6536..7090
                     /locus_tag="ACG81A_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFS5013805.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(7820..8134)
                     /locus_tag="ACG81A_000408"
     CDS             complement(7820..8134)
                     /locus_tag="ACG81A_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFS5013806.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(8148..8408)
                     /locus_tag="ACG81A_000409"
     CDS             complement(8148..8408)
                     /locus_tag="ACG81A_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFS5013807.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            8578..8892
                     /locus_tag="ACG81A_000410"
     CDS             8578..8892
                     /locus_tag="ACG81A_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS5013808.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            8894..10549
                     /locus_tag="ACG81A_000411"
     CDS             8894..10549
                     /locus_tag="ACG81A_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5013809.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            10632..12767
                     /locus_tag="ACG81A_000412"
     CDS             10632..12767
                     /locus_tag="ACG81A_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFS5013810.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            12942..13451
                     /locus_tag="ACG81A_000413"
     CDS             12942..13451
                     /locus_tag="ACG81A_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013811.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            13448..14751
                     /locus_tag="ACG81A_000414"
                     /pseudo
     CDS             13448..14751
                     /locus_tag="ACG81A_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            14859..15716
                     /locus_tag="ACG81A_000415"
     CDS             14859..15716
                     /locus_tag="ACG81A_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS5013812.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(15828..16148)
                     /locus_tag="ACG81A_000416"
     CDS             complement(15828..16148)
                     /locus_tag="ACG81A_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFS5013813.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            16302..16697
                     /gene="dhaM"
                     /locus_tag="ACG81A_000417"
     CDS             16302..16697
                     /gene="dhaM"
                     /locus_tag="ACG81A_000417"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFS5013814.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            16672..17682
                     /gene="dhaK"
                     /locus_tag="ACG81A_000418"
     CDS             16672..17682
                     /gene="dhaK"
                     /locus_tag="ACG81A_000418"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFS5013815.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            17710..18315
                     /gene="dhaL"
                     /locus_tag="ACG81A_000419"
     CDS             17710..18315
                     /gene="dhaL"
                     /locus_tag="ACG81A_000419"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFS5013816.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            18462..19127
                     /locus_tag="ACG81A_000420"
     CDS             18462..19127
                     /locus_tag="ACG81A_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFS5013817.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            19410..20069
                     /locus_tag="ACG81A_000421"
     CDS             19410..20069
                     /locus_tag="ACG81A_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5013818.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(20146..21756)
                     /locus_tag="ACG81A_000422"
     CDS             complement(20146..21756)
                     /locus_tag="ACG81A_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5013819.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(21769..22650)
                     /locus_tag="ACG81A_000423"
     CDS             complement(21769..22650)
                     /locus_tag="ACG81A_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5013820.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
BASE COUNT         7561 a         4063 c         4858 g         6180 t
ORIGIN      
        1 gcttcataat aaaaaactgt caaaaaaaga aaaaaagaat aaaattttga cagtttcttt
       61 ttttaaaaca gtagtatgat aagagtagtt aaagaaagcg tttgcatata atggagggaa
      121 tcacaatgac agaatcaacg tatatcatgg cgattgacca aggtacaact agctcacgag
      181 caattatctt tgacaagaaa ggaagacaca tcggcagttc tcaaaaagag ttcacgcaat
      241 atttcccgca agaaagttgg gtggaacatg atgcaaatga aatctggaat tctgtacagt
      301 cagttattgc cggagcgttc atcgaatcag ggatcaaacc aaatcagatt gctgggattg
      361 gcataacgaa ccagcgtgaa acaacagtaa tatgggaaaa agatacagga cgtccaatct
      421 atcatgcagt tgtttggcaa tccagacagt cagctggaat tgcagataac ttaaaaaatg
      481 aaggctatca agaatttttc cacgaaaaaa cagggttagt gatcgatgcc tatttctcag
      541 caacgaagat acgctggatt cttgatcatg tagaaggcgc gcaagaacga gctgaaaaag
      601 gcgaattgat gtttggaacg atcgattctt ggttagtatg gaaactgacc gatggacaag
      661 cacatgttac tgattactcg aatgccagtc ggacgatgtt gttcaatatc catcagctag
      721 attgggatca ggagatttta gatttactaa atattcctcg agtgatgtta ccaaaagtca
      781 cttctaactc agaagtttat ggttatacgc aaggttatca tttttatgga agcgaagcac
      841 caatttcagg gatggcagga gaccagcaag ccgcattatt cggacaaatg gcattcgaac
      901 caggcatggt caagaatact tatggtacag gttcatttat tgtcatgaat accggtgaaa
      961 aaccgcaatt atccaaaaat aatttattga cgacaattgg ttacggcata aatggaaagg
     1021 tttactatgc tttagaagga agtatttttg tggctggttc ttcgattcaa tggctgagag
     1081 atggacttca aatgttgcaa aaagctagcg attcagaaga cgcagcgaaa cgctctacaa
     1141 gcgaagatga ggtttatgtt gttcctgcat tcgttgggct aggtgctcct tattgggatc
     1201 aggcagcacg aggggctatg tttggcttga cacgtggtac gacaaaagaa gatatcatta
     1261 aagctacttt acagtcgatt gcttatcaag tgagagatat catcgatacg atgcaagatg
     1321 acactggaat caagattcct gtattgaaag tagatggcgg tgcagcaaac aatgaatatc
     1381 tgatgcaatt ccaaacagat atcttgaatg taccgatcca acgagcagaa aacttggaaa
     1441 caacggcgct aggggcagca tttttagcag gattagctgt cggttattgg aaagatacag
     1501 acgagatccg cgaattttat gaagctggca agctatttga agttcaaatg gaggaagagc
     1561 gccgagaaaa actttacaac ggttggaaaa aagcagtaaa agctacccaa gcatttgaat
     1621 aaggaggaaa tccatatgtt ttcaaacaag acaagacaag atagcattca aaaaatgcag
     1681 caagaagaat tggatctgtt gatcatcggt ggcggaatca ctggtgccgg tgtggcagtc
     1741 cagtcagcag catcaggaat caaaacagga ttgatcgaaa tgcaggattt tgcagaaggg
     1801 acttcctctc gctcgaccaa acttgttcat ggcggtattc gttatctgaa gacatttgat
     1861 gtggaagtag tagctgacac agttggtgaa cgtgcagtcg tacaaagtat tgccccacac
     1921 atcccaaaac cagatccaat gcttttgcca atctatgagg gcgaaggagc aacaaccttc
     1981 aatatgttct cggtcaaagt agcaatggac ctttatgaca aactggcaaa tgtgacagga
     2041 accaaatatg agaattatac gctgacacca gaagaagtat tggaaagaga accgtttttg
     2101 aaaaaagaag ggctaaaagg cgcgggtgtc tatctagatt tccgcaacaa tgatgcccgt
     2161 ttagtgattg ataatatcaa aaaggctgcc gaagatgggg cttatctagt aagtaaaatg
     2221 aaagcggttg gctttttata tgatggcgat caaatcgttg gcgtcaaagc tcgtgacctg
     2281 ctgacagatg aagtgatcga aatcaaagca aaattagtga tcaatacgag tggtccttgg
     2341 gtagataaag tacgaaactt gaattttacg cgtccagtct ctcctaaaat gcgtccaact
     2401 aaagggatcc atttagttgt agatgcgaaa aaactgcctg taccgcaacc cacatacttc
     2461 gatacaggaa agcaagatgg gcggatggtt tttgctattc caagagaaaa caagacttac
     2521 tttggtacga cagatacgga ttaccaagga gattttacgg atccgaaagt cacacaagaa
     2581 gatgtagatt atctattgga tgtgatcaat catcgctatc cagaagcaaa tatcacattg
     2641 aatgatatcg aagcaagctg ggcaggactt cgtccactgt tgattggtaa ttctggttct
     2701 gattacaatg gtggagataa tggatcgatt tcagacaaga gcttcaataa agttgtcgat
     2761 acagtaagtg aatataagga aaacaaagtt tctcgtgctg aagtagaaga cgtgttgaac
     2821 catttggaaa acagccgtga tgaaaaagca ccttctacga tttccagagg tagttcttta
     2881 gaaagagaac cagatggttt gctgacttta tctggtggga aaatcaccga ttaccgtaag
     2941 atggcagaag gagctttgcg attgatacgc caattattaa aagaagaata cggaatagag
     3001 acgaaagaaa tcgactctaa aaaatatcag atttcaggtg gaaacttcga tccaactaaa
     3061 ttagaagaaa cagtgacaga attagcaaaa gaaggagtag cagccggttt agaggaagaa
     3121 gatgctactt atatcgctga tttttacggg actaatgccc gacgtatctt tgaattagca
     3181 aaagaaatgg caccttatcc tggtttgagt ctcgctgagt cagctcggtt acgttatggt
     3241 ttagaagaag aaatggtttt ggccccaggc gattatctca ttcgtcgtac aaatcatctg
     3301 ttgtttgaac gagatcagct tgatgagatc aagcaacctg tgatcgatgc aattgctgga
     3361 tattttgaat ggacagaaga ggagaaggcg caacagacta aacgtttaga agcattgatt
     3421 gcagaatcag atctgcggga actaaagggg gagaaataaa tgaacagtga tatgactcaa
     3481 atcatgggag agtttattgg gacactgatc ctagttttgc ttggagatgg tgtgtgtgcg
     3541 gctgtgaatt taaacaaaag taaagcacag gcttctggct ggattgtcat cgcatttggt
     3601 tggggactcg ctgtaacaat ggctgtctat atttctggat tcatgggccc agctcatttg
     3661 aatcctgcag tctctctagc tatggcgatg acaggggcaa ttagttggaa tcttgtcgtt
     3721 cctttcatca ttgcgcaagt tttaggagct tttgcagggg ccattcttgt ttggctttct
     3781 tatttgccac attggaatgc aacgaaagat gaaagcgcga ttttaggaac gtttgcaaca
     3841 ggtcctgcta tacggaatta tccagcaaat gtgatcacag aattaattgg tacatttgta
     3901 ttagttcttg ggttgctagc ttttggacaa aacgaatttg cacctggaac gaacgtattc
     3961 gctgtaggtg gattgatctt agccatcggt ctttcattag gagggccaac aggatatgca
     4021 atcaaccctg ctcgggactt tggtcctcga ttagcacatg cggtattgcc aatagctaat
     4081 aaaggaactt cagattgggc atattcatgg gtaccaatcg caggtccaat gatcggagca
     4141 attatcgcgg taggtgtcta ttcattaatg gtgtaaatct ataaaacata ctaaagtttg
     4201 acaaccaaaa tgtgtcttgc tatagttaag tggaaaataa ataagaccta gaagaatggg
     4261 gtatgttttg tgagtgtagc tggtatctaa tcagtaagtc gaatagccaa taaacaaagt
     4321 ttatttggtg tgcttatgaa acggtacctg tcaggaaaac aatatctttg tattctaata
     4381 aggataggac atgacttttt taagctgtgt tctatccttt tatttatctc taggccctta
     4441 tttattttgg gacaggtatc tcttcaagaa aaggagagaa tatggacaac gaaatgatta
     4501 gtcaattaca atttgatcgg atcaaaaagg aaatccaagc aagagcaatt ggaaactata
     4561 gcaaaaaacg aatcagcgat atgacggtct cgacaaacct acaaactgtc ttggatcgtc
     4621 aagaagaaac aagagaagca cgcttgatct tagaaagcag tcagcatgtt ccatttatgg
     4681 ggttgccgag gatcgatgca ctgacagaac aagtcaaaaa agggttgatt ctgcagccaa
     4741 ccgatttgat cgaatatgcg gattttctta gaagcagtcg gatgatcaca aagttttttg
     4801 ataagaatca ataccagaca cccttgttgt ttgcttatag caaacatctt cctgatttga
     4861 taaatgtaga agagttgatt gaccagaaaa taaaaaataa taaagtcagt gacgacgcgt
     4921 cacgtaattt gcgtaaagta cgcaaacaat tacaactaat cgaaaaagaa atccagtcaa
     4981 aattactgaa attcttgcgt catccgaaaa acaaagaaat gatccaagaa gcaatgattg
     5041 ttcaaaaagg cgaatactat acgattccta tcaaagccag ctataaaaat aaaatagatg
     5101 gaacgattat cgatgaatca aataaaagaa cgacggtttt tattgaacca actgtgattt
     5161 ctaaattgaa tgaacactat cagttgttaa aagctgaaga aatcagtgaa gaatatcaag
     5221 tcctggcggc attgactggt gctatcgcag aaaacgaaga agtgatcgat ttattgatcg
     5281 aaacgatcac ggtgttagat atcatctttg ctcgggcaaa atttagccga gaaatcaatg
     5341 gaagcacacc tagaatcaat aaatcggaac atatcattat caagcaaggc agacatccat
     5401 ttttacctga gcatgcggta ccattggatg ttgaaatcgg taaagactat cgaggcttga
     5461 ttatcaccgg agcaaatgcc ggaggcaaaa cagtcgtgtt gaagacagtc ggtttattga
     5521 ctttgatggc gatgttcggt atgcaagttc cggcaaaaga agggacagag cttgctgtct
     5581 ttgatgagat tttcgtcgat gtcggtgacc accaaaattt agaaaatgcg ttgagtacat
     5641 tttctggtca tatgcaaaac atcgctgcta ttttgaaaaa gataaaaagg aatacgttgg
     5701 tacttttaga tgagattggc agtggaacag agccaaatga aggggctgca ctagcgatcg
     5761 caatcatgga atctatgtat gagcaagggg cattgatcat tgccacgacc cattatggag
     5821 aaatcaagaa attcgccgga gatcatgaag attttgtgcc ggctgctatg gctttcgatc
     5881 gggaagcact tcgtcctaaa tatcagttgc gggttggaga aacaggagaa agccaagctt
     5941 tgtggattgc tcataaaatg gatatgtcga tgaaattgat ccatcaagca gagcgttact
     6001 tggcggaaaa agcctatcgg acacagaaaa aagaatttct tcctaagaca gcagttacaa
     6061 ataggaagga gaataaaaaa gaacgccagc tctttgcaaa aggtgatcgg atatttgtca
     6121 atgaatacca aaaagaagcg ttagtttatg aagatatagg tgaagatacg attgatgtat
     6181 atttagacaa aaaaataatc cacgtgcctc gtcagcgtgt tcgtctggtg cgttcagcag
     6241 aagatctgta cccgacaggt tacgatttag acagtctgtt tatcgattat aaaacacgta
     6301 aacggcagcg tgacctagag cgaggatcaa aaaaagctca taaagtacta gtaaaagaaa
     6361 tgcgcaaacg acaggaagaa agaagagtaa acgatgaaaa cagcaagtga ttatctcaaa
     6421 ttagctcata atatcagtgg ctgtaaagaa aaataaaaac agtgaaatca ttggttttat
     6481 tccccgtgta agctaaaata aaaagaaaag agtgctgagg aggtagggcg ataaaatgaa
     6541 attaagcaca gactggcgta aagaaataca aacgattccg aatcttttat cgatattccg
     6601 tatttttttg ctgccgattt atctttattt tgtgctgaga cagtcatttt atatagcagg
     6661 agcggtcatt gttgtctcag gtttgtctga ttatctagac ggggtcattg ctcggagata
     6721 taatcaggta acagacttag ggaaggtgct tgatccgttt gcagataaat tgacacaact
     6781 ttttctgatt ttgtctatgg catggtatcg accgtggctt tggctgttgt ttggtctatt
     6841 cttgataaaa gaaggcttta tgtttgtagc tggattaatc gggctatcta aaaatatcaa
     6901 attaagtgga gcgaagtggt atggcaaagt agcgactgct gtcatctatg ttggcatgat
     6961 ccttttgctg cttttcccag aattaccaac gctgtgggtg agagtcattt ttgccgtcat
     7021 cacttacggt cttttacaat cttttgtctt gtatgctgtc gagtaccgaa agatgtttca
     7081 aagaaaataa aaaaggggtg tgacctatgt cgcattcctt tttttgataa atagactccc
     7141 agtagctact tctcgataat aagccaacca aaccaaaaac aggagaagtt gtttttgaaa
     7201 ttatttcttg tgattcggag ttaaatggta ggctcacaac ctcttggtaa acggtgttca
     7261 atcagctgac cttcggtatt agctcaaaaa acggcaaaat atgagcagct atttttccgt
     7321 ttgtttctct aatactcagg tcaaaccgct gattttacaa cctcttcctt aaacggtatt
     7381 caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtagaaa tccattttca
     7441 caaaattttt cttaatactc gaaactaagc gactttttca caatctcttc cttaaacggt
     7501 gttcaatcag ctgaccttcg gtattagctc aaaaaacggc aaaatatgag gagctatttt
     7561 cccgtttttt tctctaatac ttaggtcaaa ccgctgattt cacaacctct tccttaaacg
     7621 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata tgagaagcta
     7681 tttttgtttg gctgttctta ttacttgatg caggacagcg ttttcacaac ctttgattca
     7741 cggttttcca gaagtagctt cttcgaaaaa taagccgaaa tttcttgaag ctgatcactt
     7801 ctgtcgcaac ctctactgac taaggcttga tataagcata accttgggat tgcagttcaa
     7861 ctaaatcaag gacacctgct ggtacaacga ttacttgttc aggtaaagaa ctttctgaaa
     7921 tatgattagc acgcatagca ttggcgcaag cgtgaaaaac gactccttgc aaatctaaga
     7981 attctgcatt ggcagagtct agataaccgg taatcgcgat gccatttaca gatacgacaa
     8041 tcgtcgtttc tggagaagct tttatcaggt tttttacgtt tttccctgtt tctgaccatt
     8101 tttctaattc atcaatatgg aaaacgactt tcattgaaat cattccttta ctttttaata
     8161 atcatcttga tcgcttgttc tagtttagcc gcttgttctt ctggattact atcaggtttc
     8221 tcaaagcaat ttttcagatt atctgcaacg atcatgccca ttacgcgatc aatactagaa
     8281 cgaacagcgc ttaattgagt gatgacatcc atacattctt tttcgtcttc gatcattttt
     8341 tggataccac gaatttgtcc ctctgtacgt ttcaaacggt tcaggatatt tttattatca
     8401 atctccataa cacattctcc tctattttta taattagata atacccctta tcggtatttt
     8461 atgcaaatat aaaaagtagt gaaaaaaatg aagagtcatt ctggttgaca tattaataaa
     8521 gctcctgttt aatatacccc atgaggtatt tataaaaatg taaatgaggt gcagaaaatg
     8581 aatttgctat ttcaatcaat aaaatcgata acagtcccag aattaaagga aaagcttttg
     8641 gaaaatccag cgttacttga tgttagaaca cctgcagaat accggggcgg gcatataaaa
     8701 ggagctaaaa atgttccatt acaaagtata aacagatatg atggagacaa agaaaagacc
     8761 gtctatgtga tttgtcaatc agggatgagg agtaaacaag cagctaaaga attaaagaaa
     8821 tctggttatg atgtagtgaa tgtacgtggt ggcatgaatc aatggtttga tcgaacagta
     8881 ggagggaaat aagatgaaaa tcgttattgt cggaggtgta gcaggtggaa tgtctgctgc
     8941 tacacggctt cgccgattaa tggaagatgc agaaatcgtt gtttttgaaa aaggaccgta
     9001 tgtttctttt gcaaattgcg gcttgcctta ttatctttcg ggagaaatca gtgaacggga
     9061 aaatcttctt gtccaaacgc cagaatcatt atccgctcgt ttttgtttag atgtgcgtcc
     9121 aaatcatgaa gtgacagcca tctttcccga aaacaaaacg gtagaagtcg tacatgaggg
     9181 tcaaaaacac attgaacagt acgatgcatt ggttttatct cctggtgcaa aaccagttgt
     9241 tccatcgatt ccagggataa cagaagccga caatgttttt tctattagaa atgtaccaga
     9301 tatcgataaa gtgatgcatg cattagaaaa acagccaaag cgtgccgtga tcgttggtgc
     9361 aggattcatc ggattggaaa tggcagaaaa cctaaaaaga agaggtttag aagtcatggt
     9421 gatcgaacaa gcaccacata ttcttccaac actggatgaa gaaatggcag cttttataga
     9481 aaaagaattg tctcatcaag gagtagaagt gattacttct catgctgtcg ctggatttga
     9541 agaccacggg aaacgattgc gactggatga tgggcgtacc atccctgctg atttagttat
     9601 tttatccatt ggtgttcgtc ctgataacca gctagcagtg accgctggaa tcgaattagg
     9661 catacgcggg ggtatcctag tagacgaacg ttatcaaacg aatattcctg atatttatgc
     9721 ggtgggggat gctatcgttg taaaacagca aatcactgga aaagatgcac ttatttctct
     9781 tgcttcacca gccaatcgtc aaggtagaca agttgcggac acgatttccg gaatttctcg
     9841 aagaaatcaa ggcggtattg gaacagcaat tatacgaacg tttggaatga ctgccgcatc
     9901 caccggttta agtgaaagaa cagccaaaga aaacgaactg tcttttgaag tcattcatgt
     9961 atcaggaaaa gatcatgcaa gctattatcc agaagcaaca gatattttac tgaagttgat
    10021 cttccatcca gagactggcg agatttatgg tgcacaaggt gttggggcaa aaggtgtgga
    10081 taaacggatc gatattttag caacagcaat caaagggcat ttgacgatct tcgatttacc
    10141 ggaattagaa ttgacgtatg caccgccatt tggctcagcc aaagatccag taaacatgct
    10201 aggatatgca gcaatgaaca ttgcagaagg gcttagtgaa accgtacaat ggcatgaatt
    10261 gccgacagaa ctagcaaaag gaaaaatttt attagatgtg cgaacagcag aagaattgga
    10321 aaagggcaaa ttcaaggaag ccaaacatat ccctttgaat gaacttcggg accgattaaa
    10381 tgaattagac agccaacaag aatatatcgt cagctgtcat agcggattac gtagctatct
    10441 agcggaaaga atcttgaagc agtctggcta ccacgtaaaa aaccttgatg gtgcattttc
    10501 tttatatcaa actgtccgac ctgaagaact gatatatcct aacaaatgat actcgttaaa
    10561 aagcttgcat tcgactctta aaagaggtaa actaatcggt tccaccactt taaaaaaaga
    10621 ggagaatgtg tatggtcaac acacaaaaac agttagaaga agcatatctg aagaacgtgt
    10681 atcaaaagct aattgataaa aaaaacgaac tgcgggaatc gatggatacg gcaaaaatag
    10741 aaggaaacca gtcgttgaaa gaaatggctg gcgagcttcg attgaatttt gacagttact
    10801 tagataacct ggatactttt tcgatgattg aaatgaaaaa tagagaaatc gatcagatga
    10861 atatcaaaca gaaaaacgca gaaaatcagc tagcaaagat cgaacgttta ttgaaaagtc
    10921 cctactttgg aaaagtcgtt gtagactttc tggataatga gccaaaagaa tccttttata
    10981 tcggtacagc aaatttcaca gatgatgcag atgaaaatct tgtttatgat tggcgttcac
    11041 caattgccga acttttttac aataacatga ttggtccatc ttcttatacg gttcgtcaaa
    11101 ataaaatcga tacggttatt aaagaaagac gacaatttct tttggaaaga gatcgtttgg
    11161 tcaagttttt tgatacgtct gtagctattc aagatgacgt attattagaa gtcttaggcc
    11221 aagatgagac caatgaaatg aaggctatca ctgctacgat ccaatcggaa caaaacacaa
    11281 tcattcggga tgtgaaaagt caaaatatct tggtaaatgg cgtagcagga agtggtaaaa
    11341 cctctgctat catgcagcgt attgcgtatc tgctctattt atatcgagat acgatgacat
    11401 ccgatgactt gctgattcta tcacctaatc atcggtttat cgactatatt tcgaatgtcc
    11461 taccatcatt aggtgaaaga aatccactga atctaaccat catacagctg gtttcacagc
    11521 tttcagcaga ggaaatagaa ggtgaagagg cttatttcaa acgtatcacg ggagaaaatg
    11581 tatcggagca gacagaacgt ttgcgtagca aaaagtttat cgataatcta aaacaatcag
    11641 atcctttgtt tttagatcat ccgaatttta tccgtggact tactaaaaat ggaaaaacag
    11701 tcctttctaa aaaaacgatc gaaaaaatat atgagaaagt tcctgctcat cctaaattga
    11761 tcgataggct tcaagcaacg aaaaaggcac tgatgagcga atggaagaat catcttttga
    11821 aacaagcaaa aagtcctgct gtgcagaatc aggtactctc gttgacagaa gataggcagt
    11881 tggaattatt cggtaaattg atttctgatg attcggaaca aagtattgta gcttatgcaa
    11941 ggaaactttt acaaaaaaaa tatcgaaaaa taaccagaca aatcgaagaa atggcatggg
    12001 tggatgagca tcagttgttt gaaagaattt atgaaaaacg atatggctca gcttatgctt
    12061 ggcaacccac acgtactgtt gatgaagcag tcattatatt agctattcgt catttacttg
    12121 ttgaaaaggt aaatgtacca gcatttcgct atctattgat tgatgaagta caagattata
    12181 ccctggcaca attaggtcta ttgatcgaat tatttccaaa aactcacttt actttagtag
    12241 gtgacgagaa tcaggcaatc tttaattctt ccactacttt tgctgatatt atgcgttgtt
    12301 tcgacgacta ccatctgccg atccatcggt atgatcttag aaatagttac cgttctagcg
    12361 gagcaatcac agagcttttc aaaacctatg ctgtggatca agagaagatc gacatcgtat
    12421 cgatccgacc gcaaggaaaa gaacccgaat accgctcttt tcgatcctct gaagaattgt
    12481 tgaaaatact agctgagatt ttggtttctc taaaaggaga aaaagcggca gttatcaccc
    12541 aaacagaaga ggaaacccaa acgctacaag aatcgattaa aaaaaattca tatggattag
    12601 aaaactgcca aatcattcct ttgagtctag caaaaggact ggaatttgat catgtaatct
    12661 tatatccatt tgaaaacgat ggagatgagc aaagaagacg cagacaaatg tacacggcga
    12721 tatctagagg aatgaaaagc attgtcgtat tggaaagagc aacataatta agagataaaa
    12781 aataaaaaga acaactgata aaaagaacaa aatatttttc atttgctgat gagaaatgtt
    12841 ttgttctttt tcttaggaaa aatttaggaa cactttatgt gaaaagaaat gaaatgtatg
    12901 gtaaactcct acgtcgtaac aaaaaaacgt gaggtgagga tatgaaaaaa gtaacaagag
    12961 aagaagttgc atcaattttg gtgaaagata agtatttttc aaaaggtaat tactggttaa
    13021 agatttggca aacgttagtg gctcttttag gatggatgat cgttgttctt ccttttatat
    13081 gggtcttttt cccattaacg agaccagaaa gagccaagga tttttccttg tacgcttttt
    13141 tatcagaaaa gaaaatgctg tatttcttaa tcggcttttt tgtattgatt tttttcagtt
    13201 tcctgattac ctcatttgta ttgactaggt ggaacaatcg aaatctatac aaaaaagtaa
    13261 ataaatcttt tgaatatgaa gatgaaaaat tgaaaaaaag acaagaatta cttgaagaag
    13321 tgtatacgga acgttttgga acaaaagaag agagagaaac ggtccgtttt tattctgtct
    13381 ccgaagagaa aaatctagat acgacattca ttgcagattt gtataaagaa aatggggtgg
    13441 aactgaaatg atctcaatca cccatacagg taatttattc ttagatactt gtttgtctat
    13501 tatgtatttt ttctcgtttc ttatccgatt ttaggcggat ttgtatggtt tattggtgtc
    13561 tggtgttatg tgtttttata caaacataag caaaaagaat gggtagatgt tcccttgagt
    13621 gtcgaaccgt tcatcacgat catggtgcca gcccataatg aagaaattgt gatcgaagat
    13681 acaatcgagt atttgatgac gaaattgaat tatcacaact atgaggtatt agtgacagac
    13741 gatggttcaa cggaccaaac accagaaata ttagctagac tgatgaaaaa atacgctaat
    13801 cttcgtgtgg ttcggatcga aaaaaataaa ggaaaagccc acgcgtttaa tatcggactt
    13861 gcctttgcaa aagggaaatt gattttaagc aatgatgcag acacggtacc ggagccagat
    13921 gcactgatta ggtatgtgaa ttatttcatt cgccctggtg caagacatat tgctgcggtc
    13981 actgcaaata tggatgtcca aaatcgtacc aaattgattg caaaatctca aacggttgaa
    14041 ttctcaagta tcgtgggcat cattaaacga acacagtcag ctgttttcgg tggtctttat
    14101 gcgtacagtg gagcaaatac gatgtatcga aaagaagcct tgatcgatgt gggcggattc
    14161 agacaagatc gagcgacaga agacatcagt attgcttggg atcatcagtt aaatgactgg
    14221 ttatctgttt ttgctcctga gattatcttt ttcatggaag tacctgtgac gttgaagatg
    14281 ctatatcgac aaagaaaacg ttgggcaaaa ggtggaacag aagtctggct gacgaatttc
    14341 aaaaaagtga tgctccatcc ttttaaacat attggaagaa cgatcatctt cattgatcaa
    14401 acattgagta ttgtctggtc aatattcttt tgtatttccg tagttctttt tgctggatta
    14461 atcgggcatt atgtatatca aggaaactac gaacagattt atattacttt tacattcagt
    14521 tttgtgttca tctgctttga aatggtcgca ggatttttcc agttgctagc ttcgctgatt
    14581 gtagatgatc gacgaagaaa attgaaatac cttcttttcg caccactata tatgcttttg
    14641 ttctggatag tgaacgcgat aacgatcgtg acgactttca ttccagcagt caaaacgatt
    14701 ttaggttatg gaagcgggac atggaaaagc ccggaacgta caaaaaaata aaagatgcac
    14761 cgttttttag acaaacagcc tgttgtgttt atggttcttc caaacatttt tgcttataat
    14821 aagaaattag ttaaaaagta gtaaggatgg gaaaagtgat gaaaaaaaga atgaagccgt
    14881 taatctggcc aattgctgtt tccagtattt tcttattggg acagacagct tttgcggaaa
    14941 cttctgaacc tgctcaaaca gaaccttcaa caactataag ctcttctggt gctgaaaacg
    15001 gtgcctcgga aacagaaatg acttccaatg taccgacaga taattcagga gaaacgattt
    15061 catcttcaga agatacatca ataaatagtt ctacctcttc tgaccaaatc gatcatttgc
    15121 aagacttgga aaataaaaga aaagaactcg aagaagcatt agaagctgca aaaaacaaat
    15181 tgaaagaaga ccaagcagca atcgatcaag atttagctga tcttaaaaat aaagtgggaa
    15241 atgaccgtgc acaacttgag aaaaagcttg ccgaagcgaa agcaaaagcg gaagctgtcc
    15301 aaacagatct tgacgggaag atccaacaag caaaagaatt caatcagcaa ttaaatcagg
    15361 ttgctcaaac agcttctgga aagctaagtc aaactcaagc ggatatcaca gcggccagtc
    15421 aagcaatcca cggaaagatc agcgaagcac aaacagttgc taatgatttt gaagcaaaaa
    15481 aagctgactt agcagctagt ttccaacgaa taaaagatat tttgcattcg gcaggagaaa
    15541 aatcagtgac ctctggaagc acgacagaaa cagttcagca aacgacagag ccatccaaat
    15601 caacaagtca gactttgcca aaaacaaatg aaaaaacagc ttctgtcagt tattccttag
    15661 caggggcggg tgtgttagtc gcagcagctt acggaatcat ccgcaaaaag aaataaaaaa
    15721 gagaggatgg gaaggtagag tacctcgaag ttagactgaa aaatctaatt cttgagtgcc
    15781 actacatgtc ccatctctct ttagttatta aaacagatgt cccctactta tctcgtggag
    15841 acagctgttc cactacatgt gaccatcagc atgccattgt cgctgcctaa tacttcatag
    15901 tccattttta cgccaatgat cgcatcagct cctaatgctt ttgcacgttc ggccatttcg
    15961 gcaagtgctt cttctcttgc acccattagt tcgttttcat agctttttga gcggccgcca
    16021 aagatatttc gcaaccccgc ctccaagtct ttaaatgcat ggatcccagt aatgacctca
    16081 ccaaaaacga tccctttata agctactacg gcttttccct ctacactgtt cgttgttgtt
    16141 gtaatcattc gatcactcct ctcttttaat taaagtataa caaacttcaa gaagcactac
    16201 acatatcttc ccaaaattag aaatttatta tatacttgaa ttgttcatta atgaaagcga
    16261 aaccaatcgt tagcaactat gtaaaagaaa ggaagattct tgtgaaaaaa agtattttgc
    16321 ttatttctca tagtcaaaaa gtaacagatg gtattaaaga aatgattgag cagatgcaaa
    16381 aatccgaaga ggtgcaaatt ttttctctcg gcggaataaa tggcgagatc ggatcagacc
    16441 cgatgaaaat cgtagaagca gtcaatgaat caagcgatgc agctagttat tttgtttttg
    16501 cggatatcgg tagcgcggta ttgaactcgg aactggcaaa agacatgttg gaagaagagc
    16561 agcagcaacg ttatcatctg gtggatgcgc ctttagtgga aggagctttt gcagcagcca
    16621 tcactgccgg tataacagat gatactcagc aaatcttaac agaagcacaa aatgccggaa
    16681 agaaaggatg ggaataaatt gaaaaaaatc atgaatgatc caagcaatat tgtcgaagaa
    16741 atgctggaag gattagtaaa aagctatcca gaacttgttc atcgtgtaga atcttcccgt
    16801 gttgtagcca agaatcagaa agcggaacaa gttggtttgg tttctggtgg aggaagcgga
    16861 catgaaccat cccatgccgg gtttgtaggg gaagggatgt taagtgcagc ggtgcttggt
    16921 gatgtgttta cttctccaac tccagatcag attcagacgg ctattaagga agcggatagt
    16981 ggaaaaggag ttttgctgat cgttaagaat tatacagggg atgcattgaa cttcgatatg
    17041 gcaaaggaat tagctgctat ggacgatatt gaagtagaaa gtgtgatcgt agatgatgat
    17101 atcgccgttg aaaatagtac gtatactgct ggaaaacgcg gagttgcagg tactgtcctt
    17161 gtacacaaaa tcgtgggcga tgcagcaaga aacggtgctt ccctagctga gttgaaagaa
    17221 ctgggagaaa aggtcgtaca ggcaactaag acgatcggtc tagcgcttcg agcagcaact
    17281 gtaccagaag tagggaaacc aggattcgaa ctaggagaag atgagatcga gtatggtgtt
    17341 ggtatccacg gtgagccagg ctatcgtcga gaaaagatgc agccttctaa agtactggca
    17401 aaggaacttg ttactaagat tttagatgat tactcgaatt taccaaaaga agcaggtgta
    17461 ctagtcaacg gaatgggcgg gacgcctttg atggaacagt tcgtgttcat gaatgatgtt
    17521 ttagctctgt tggaagaaag aggcgtcaat gtagttttcc gaaaagtcgg taattttatg
    17581 acctcactag atatgcaagg tctttctcta acactgatcg acttgacgga gacacaatgg
    17641 aaagattctt tagaaagcaa tgtacagacg atttcatggt aaaaaatctc aagataaaag
    17701 ggtgataata tgaaactaac cgttcaagaa atccaagaat ggctcacgca atttgcagag
    17761 gcaatcaatc agaacaaaca atatctcagt gatctagata ccccgattgg cgatggagac
    17821 catggaaata acatgggaag aggggtttca gcttatgaag aagcttttca agccgatcat
    17881 cctgaaacaa tcagcgatac attcaaagta ttctctatgg cgatgatatc gaaagtcggt
    17941 ggggcttctg gtccgttata cggatctgca ttcatgaaca tgatgaaagc gacaaaagag
    18001 gtcgattcta ttgattctca agaaaaatta ggcgaagtga tcgagcaggg aacagcgggt
    18061 attcaaagcc gaggaaaggc agaagctgac gataaaacga tgttagatgt ttggctgcca
    18121 gtcacagagg cattgaaggc agggaattta acaaaagaaa tcattgacca ggcaaaagaa
    18181 cacaccaaag tcttggtagc taaaaaagga cgagcttcct atctaggaga gcgtgcaatc
    18241 ggacatatcg acccaggagc tgcttctagt gccattttat tttcaacgct tttagatgtg
    18301 atcaatcaac cttaagttca ataaaactgc ggtgatgctc atcgcagttt tttgtttggc
    18361 tgttcttatg acttgatgca gaacagcttt ttcacaacct ctatttgtcc atatcgcaaa
    18421 attcaggtag aatacaaaag ataacggaaa gaggcgaaac aatggaactt ggaaaatcag
    18481 attatcagtt gtttgatcga ccaatctacg cattcaaaca gctcaaagag agccatccaa
    18541 cagataagat tgaacaaatc aagaaagaat ataaagaaca ctggcaaaaa tggaaagaga
    18601 ttcagctcca gacagctgca cttttgcctg atatgtacgg catgagcaaa cctaaaatcg
    18661 aaagctggac gaatggctgg aatttacgca gtcatttttg gagcgcgtac cgaagcgaat
    18721 ctcgtcaaga cgagaatgcc tgtctagcag ttttgttgaa tcaaaaacaa taccaaatct
    18781 atttgatgta ccaacattat aaaagcgaag aacgatatgg tagcattgca gcgtataacc
    18841 agttgcttgc gatactgaaa gagtggagca aaacagtcga tatcaaggat tattacatat
    18901 ggccgcaacc agaacatgaa cttgacgacc acttagcttt gtctgactat ttgtccgata
    18961 caaagaaaca agaagagttg aaaaaagcga tgagagaccg aacctttcaa atgggtaaac
    19021 tattcttcta tccgcaagaa atcgaacacg ttgagcaaat aaccgctgaa accttacaag
    19081 aactagcacc actatatcaa aagctaggtg cggaaaaatt tcaatagaca acacgaaata
    19141 agcaatgcga aaaacaaacc ttactctcgg cttgcgagtg aaggtttgtt tttgattatt
    19201 caaaaagtaa atatttaaaa aattgatagg aatcaattac ttaaggtctc aaaagaagga
    19261 aaataaagca agggacaaaa agaaatccac tctatttgct ggtatctggt tcgcttgacg
    19321 tttatgctat gataaaagaa aattatcagt tggaataagg gaagttatgt ttctttatta
    19381 gaaatagctg tttagaagga ggagagatta tgagtcatga tcatttatgt gtttcatttg
    19441 ttcctttgtt caatcactta gtacagcaag atcaagaaaa agtacacgca cttgtcaaac
    19501 atcactatgt ggaaaaagga gaacagattc ttgcgccgaa tggagaacct caattagtga
    19561 tcgtggcctc cggcagtatg aaagtgtatc aattatctgc tagcggaaag gaacagcttt
    19621 tacgagtaat agagccgggc ggatatgagg gagagagcca gttgctaggc gttccaaatg
    19681 agtctttatt tggcgaagca ctagaaaaaa cggaaatttg catattgaga caaaaagatt
    19741 tctcggaact cttactgcat tatcccgccc ttagtctgaa acttttagca atcaacgttg
    19801 agaaattaac aaaagtacag cagcagacaa cttttttgat gatggaaaaa gtagaagaac
    19861 gagtcgctgt ctatctgctt gacttagcta aagcgacagg gaaggatcac tttttcctgc
    19921 caatgaaaat gaaagaactt gctgcattta tcggtacgac ccccgaaact ttatctagaa
    19981 aatttaaatt attagaggag aatggttatg taaaaagaaa gaatcgagaa gtctggatca
    20041 ttgataaaga gcagttagaa gacttgtaaa taaaaaaatg acctccaaat gttggatttt
    20101 tgacccagat ttggaggcta gcatgaaatt gtacatgctt ttttattatc cttctagtaa
    20161 gtctcttcgc ctataaccaa tagaaccaag gacgaacagt gcaaggctga tacatgataa
    20221 cgtaaggaac acggaaagat caaaagaatc gtttggcatc cgtggaaccc agctagggac
    20281 agctgttttg gaaaaccatt ctggtaaatc caaaattccg ccaaagtaat tgagtgcaaa
    20341 ggagtaagct aagtagatat aagcaagttt acccattcga ggaagccatc cgaatagaaa
    20401 gccactaatt ccgataaaca ctaaaacggc aggcaggaaa ttgaacccgg ctttaagaaa
    20461 catcaataga tccatgtctt tcgcatcctt catcgaagcc aaagcagaaa gccctagtgc
    20521 accagaacta atcaggattg ccaatatagc aaatagaata gctagtaaca tattggtcca
    20581 ataaagttgg ttcctagaaa tttttgttgc gaacagctga ttcatacgtc cgtgggtttc
    20641 ttctgtaaat aaacgattga tgctagcaat tggtaaaata gcagctagac aaatcaagac
    20701 gaccatgatc gtgcttgtaa agtttttttc aattgagacg ccagattgga taaacatctg
    20761 tttcattaag tcgttacttt ctaaaaactg ttgcatattc ccgtagatag agccgtatgc
    20821 cgcccccatc aaaccaaaag tgataagcca actgattgtc atccctttat ttagatagaa
    20881 taataaacca cgcaaggata aaagagaacg tttagcatac ggttttcctt cacgttcggg
    20941 gatatagccg gtgttcaaat ctcgatgttc ctctaaaaca aatgaactga ttatcaagat
    21001 tagactgaac aaaaacaaat aaagcaaagg aatccagttg ttagttgtaa atggataggt
    21061 caaataagac cagcctaaag gattccacat cgaaagggag gaatcagcta catctgttat
    21121 tgctcgtaga aggtaaagtc cacctaaaaa cgctaaagac agaccattcg aactggctga
    21181 acttggcatc agttgtgcag caaacagtgc aacggcactc cccaaaatcc ccatactacc
    21241 tacagatagc ccaaaaagcc aagcaccttc tgcagtgatc gaatctgctt ggaaaaaaat
    21301 catcgtacag ctgatcgata ttgctaaaag caaattgatc agaatacttt cagcgatgac
    21361 agctaaagag tttgcttgac gtccgactcg aaaggaacga ataagttcaa tcagaccaag
    21421 ttcttctttt tttctcgttt ggctcaccac gtgtaaaaca gagatcacag cagcaaacaa
    21481 actgcaaaaa agcaacattt catttgcata catcgctcct aaagtatact cagcagcatt
    21541 cgaagctgga gttggtccga ccattgcaat cattgccggg ttttgcaagg tttcgtataa
    21601 tccagctaat ccttgtccgg aagtcaattc ttcaaacgca gggacaaaac caccagaaaa
    21661 agcagtgaca ccgactagcc atatcaggat ctttttccag tcacggctaa ggttttgaga
    21721 aaaaagggtg gaccagcgtg aaaatttatc agtcatttcg cttcctcctc atccttcata
    21781 atgtctcatg aataaatcct caagagtcgg tggaatagat tcaaattttt tcacacctaa
    21841 tttggacgct tcagctaaaa tcacatcgaa agcttcgtta tcagcagcaa attgagcgag
    21901 gtgacctttt tgcacaaagt catggacacc gttcaattgt gctaagtgac tattctctgc
    21961 ttctgtttct atagtgatgg tcgaacgagt caaatgacgc aattcatcga gtgttccttc
    22021 ttctacgact gttccttcgc gtatgatcac gactttatcc gctagtcgct cgacttcact
    22081 gagaatatga gaagaaagta ggatggattt gccctgttct ttcaattttt caacttcttc
    22141 ttggaaaatc gtttccatca aaggatctaa accagaagta ggctcatcaa ataaatagag
    22201 atccgaatca acagccaaag cagcaatcaa gcccactttt tggcgatttc cttttgaata
    22261 gttttttgct tttttcttag gatcaagaga gaaacgttga atcagttcat cttttttttg
    22321 agcactccct tttccatgaa gcttcatgaa gagatcgatg atcgctcctc cagtcaaatt
    22381 tccccacaaa gagatatcgc ctggaacata agaaagacgt tcatggatag ataaactatc
    22441 ttcccataca tcttgcccaa aaatcgttgc cgttccgccg tcttttttta taatccctaa
    22501 aagtgtccga atcgtcgtgg attttccaga accatttggt ccgataaatc caacgacttc
    22561 acctgaatgt acggtgaaag agacatcttt taaagcttcg aaacgaccaa agctcttttt
    22621 caaatgtgtg actttgacga tttctgccat aaaaacccca ct
//
LOCUS       DAZULY010000015        22641 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000015 DAZULY010000000
VERSION     DAZULY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.015"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..844
                     /locus_tag="ACG81A_000424"
     CDS             32..844
                     /locus_tag="ACG81A_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFS5013821.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            841..1641
                     /locus_tag="ACG81A_000425"
     CDS             841..1641
                     /locus_tag="ACG81A_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFS5013822.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1802..3070
                     /gene="rseP"
                     /locus_tag="ACG81A_000426"
     CDS             1802..3070
                     /gene="rseP"
                     /locus_tag="ACG81A_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFS5013823.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3138..4847
                     /locus_tag="ACG81A_000427"
     CDS             3138..4847
                     /locus_tag="ACG81A_000427"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFS5013824.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5059..9417
                     /locus_tag="ACG81A_000428"
     CDS             5059..9417
                     /locus_tag="ACG81A_000428"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFS5013825.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9562..10035
                     /gene="rimP"
                     /locus_tag="ACG81A_000429"
     CDS             9562..10035
                     /gene="rimP"
                     /locus_tag="ACG81A_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFS5013826.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10058..11233
                     /gene="nusA"
                     /locus_tag="ACG81A_000430"
     CDS             10058..11233
                     /gene="nusA"
                     /locus_tag="ACG81A_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFS5013827.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11255..11548
                     /locus_tag="ACG81A_000431"
     CDS             11255..11548
                     /locus_tag="ACG81A_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFS5013828.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11545..11856
                     /locus_tag="ACG81A_000432"
     CDS             11545..11856
                     /locus_tag="ACG81A_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFS5013829.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11869..14175
                     /gene="infB"
                     /locus_tag="ACG81A_000433"
     CDS             11869..14175
                     /gene="infB"
                     /locus_tag="ACG81A_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFS5013830.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14202..14549
                     /gene="rbfA"
                     /locus_tag="ACG81A_000434"
     CDS             14202..14549
                     /gene="rbfA"
                     /locus_tag="ACG81A_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFS5013831.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14706..15162)
                     /locus_tag="ACG81A_000435"
                     /pseudo
     CDS             complement(14706..15162)
                     /locus_tag="ACG81A_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            15327..17689
                     /locus_tag="ACG81A_000436"
                     /pseudo
     CDS             15327..17689
                     /locus_tag="ACG81A_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            18057..18980
                     /gene="truB"
                     /locus_tag="ACG81A_000437"
     CDS             18057..18980
                     /gene="truB"
                     /locus_tag="ACG81A_000437"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFS5013832.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18984..19925
                     /gene="ribF"
                     /locus_tag="ACG81A_000438"
     CDS             18984..19925
                     /gene="ribF"
                     /locus_tag="ACG81A_000438"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFS5013833.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20277..21062
                     /locus_tag="ACG81A_000439"
     CDS             20277..21062
                     /locus_tag="ACG81A_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5013834.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21075..21926
                     /locus_tag="ACG81A_000440"
     CDS             21075..21926
                     /locus_tag="ACG81A_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS5013835.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22050..22502)
                     /locus_tag="ACG81A_000441"
     CDS             complement(22050..22502)
                     /locus_tag="ACG81A_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5013836.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
BASE COUNT         7682 a         3954 c         4953 g         6052 t
ORIGIN      
        1 tgggaatctt ttaagtatag gaggaaaaag aatgttacgt ttttttccgc aaaaaaacaa
       61 atatattcaa gaaaaaagcg aatttcaatt taataccgat ggaccaattc ctcagcacat
      121 tgccatcatc atggacggca atggtcgttg ggcgcaaaac cgtcgtttgc cacgagttgc
      181 tggccataaa gaaggcatgg aaacagtaaa gaaagtcaca aaaaaagcat caaggcttgg
      241 agtgaaagtg ttgacgcttt atgccttttc gactgaaaac tggaagcgac caaaagatga
      301 agtaagtttt ctcatgcaac tacctgtcga cttctttgat acgttcgtac cagaattgat
      361 taaagaaaat gtcaaagttc atgtcatggg ttatgaaaac gtattgccag aacatacgca
      421 agatgctgta agacgtgcaa ttgagcaaac aaaaaacaat actggaatgg tattgaattt
      481 tgctttgaat tatggaagca gagcagagat cgtgactgct gtccaagaaa ttgccgaaga
      541 agtagcaaaa ggagaaatac acgcagaaga aatcgatgat gaattgattg ctaagcattt
      601 aatgacaggt ttcttgccta aagagttaca agatcctgag ctgatgatcc gtacaagtgg
      661 agaagagcga attagtaatt ttctgttatg gcagatcgca tatagtgagc tgtattttac
      721 aaaagcgctt tggcctgatt ttgatggtgc gcatcttgaa gaagcaatcg cctcttatca
      781 aaatcgagac cgtcgattcg gcggtgtaaa aaaaacagaa gatgagaaag gggatcagtc
      841 atgaaacaac gtgtggtcac agcagccgtg gcgctagtct tgtttattcc gattatatgg
      901 atcggaggca tagcggttga gcttacagca gcaatcttag cagtaatagg agtttacgag
      961 ctgtttcgga tgaaaggatt gacactctta agttttgaag gagttttatc cgccgttggt
     1021 gcagtgttac ttgttttgcc aaaagaaaga tggtttttct ttcttccaga gaatgtaagt
     1081 acatttatgt tattttatct tatagttatg attcttttag gaacatcagt tatttctaaa
     1141 aatacgtata cgattgatga agcaggtttt cctgtgcttg tcagcttgta tgtgggaata
     1201 ggtttccaga attttgtcaa tgcacgaaca gaaagcttgg ctgttttgat atttggtcta
     1261 tttattgttt gggcaactga tatcggagca tatatggttg gtcgacgtta tggtcagcat
     1321 aagttaagtc ctgatatttc gcccaacaaa acgattgaag gtgcgctcgg tggtattggt
     1381 agtgctgttg tagtcgcatt tctttattta ttgctatacc cagcaaaaga actatttaat
     1441 catggtatgt ttgtcatgct tctgatgacg attatttttt cagcagtcgg acaatttggt
     1501 gatttggtcg aatctgcaat caaacgtcat tatgatgtga aagactcggg aaatatctta
     1561 ccaggccatg gtggaatctt agaccgtttc gacagtttgc tgtttgtatt tcctatcatg
     1621 catttatttg gcattattta gtaagtatac ctgcgtttct ttgtgaattt cataaaagac
     1681 gcaggttttt atttttcatt atattgatac tattggtgtc tttggtatac tatggtaaaa
     1741 ttatagtgtt ttatcgttgt tttataaaaa acacaaaaaa caagtaaagg agaaaaaagc
     1801 tatgaaaacg attctgacat ttatcatcgt ttttggtata ttagtgattg ttcatgagtt
     1861 tggtcatttc ttctttgcga aacgatcagg aatcctcgtt cgcgaatttg ctatcggtat
     1921 gggtccgaaa atttatgggc atcaagcaaa agacgggaca acatatacgc ttcgcctatt
     1981 acctattggc ggttatgttc gaatggcagg caatggggat gatgaaacag aaatggcacc
     2041 aggaatgcct ctttctcttt tactaaattc tgacggcatc gtagagaaga taaatttaag
     2101 taagaaaatc caattgacaa atgctatccc gatggaattg agccgttatg atttagaaga
     2161 tgagctgact atcactggtt atgtgaatgg agacgaaaca gaagtagtaa cctaccctgt
     2221 tgatcacgat gcgacgatta ttgaaaacga cggaacagaa atcagaattg cgccaaaaga
     2281 cgtacagttc caatcggcta aattgtggca gcgtatgctg accaactttg ctggacccat
     2341 gaataacttt attttggcaa tcctgttatt catcattctg gcatttatgc aaggtggtgt
     2401 tcaagtgacg aacactaatc gcgtaggaga gatcatgcct aatggagctg cggcagaagc
     2461 aggcttaaaa gaaaatgatg aggtagtcag tgtggacgga aaagaaatcc attcatggaa
     2521 tgatctgaca acagtcatta cgaaaaatcc ggataaaaca ttagacttta agatcgaacg
     2581 agaaggtcag gtgcaatctg tcgacgtgac accgaaatct gtagagtcaa atggcgaaaa
     2641 agttggccaa cttggtataa aagccccaat gaatacaggt tttatggata agatcattgg
     2701 agggacgcgt caagctttca gcggttcatt ggaaattttc aaagccttag gatcactatt
     2761 tacaggtttc agtttagata aattaggcgg acctgtcatg atgtaccaat tgtcttcaga
     2821 agcagcaaat caaggtgtaa caactgttat tagtttgatg gcgcttcttt caatgaatct
     2881 cggaatcgtc aatctgcttc cgattcctgc cttagatggc gggaaattag tcttgaatat
     2941 ttttgaaggg attcgcggga aaccattaag tcaagaaaaa gaaggaatat tgacattagc
     3001 tggctttgga tttttaatgt tgttgatggt gctagttaca tggaacgata ttcaacgatt
     3061 ctttttctag aaaaagttga caaatagggt ataataacaa acgataacta ttttgaggaa
     3121 ataaaggagt atatagaatg agacaatcaa aaatgttgat tccaacatta cgcgaagttc
     3181 caaatgatgc agaagtatta agccatcaaa tcttgctaag agctggatat atccgccaag
     3241 tatcagcggg tatttattca tatctgccat tagcaaatcg tgtattggaa aaactgaaac
     3301 gcattatgcg ggaagaattt gaaaaaatcg gagcggtaga aatgctgatg cctgccatct
     3361 tgccagcaga actttgggaa gaatccgggc gttacgaaac atatggacca aacttgtatc
     3421 gtttcaaaga ccgcaatgat cgaaaaatga tccttggacc aactcacgaa gaaactttta
     3481 ctgatttgat gcgtaatgaa atcaattcat ataagaaatt acctttaaat ctttatcaaa
     3541 tccaacctaa atatcgtgac gaaaaacgac ctcgcttcgg tttgcttcgc ggaagagagt
     3601 ttatcatgaa agatgcgtat tctttccatg caacggaaga aagtttggac gagacatata
     3661 aagattacga aaaagcctac accgaagtat tcaagcgttg tggcttagaa tttcgctcga
     3721 ttatcggaga cggtggcgca atgggcggga aagactcaaa agaattcatg gcgatctcag
     3781 aaatcgggga ggatacgatc tgttattcga ctgaaagtga ttatgctgca aacttggaga
     3841 tggctacaag ctactatgta tcaaaaaaat cgcatgaaac acagcttgaa ttggagaaag
     3901 tagctacacc taacgtaaaa tcaattgacg aagtagcaga tttcttttct gttgaacctc
     3961 aaaaaatcat caagtctgta ttgttcattg cggatgaaca acctgtcctt gttttggtac
     4021 gcggagacca tgaagtcaat gacgtaaaac tgaaaaactt tttatctgct gactttttag
     4081 aagaagcaac agaagaagaa gcacaaaaat atctaggtgc tgagtttggt tctgtcggac
     4141 cagtcggtgt ttcagatgac gtaaaagtat atgcagaccg ttatgtccaa gatttagcga
     4201 atgccattac tggagcgaac gaaacaggat tccactataa aaatgtgaat ccagaacgcg
     4261 atttcactgt actttcttat gaagatcttc gttttgtaca agaaggagat ccttcaccag
     4321 acaacaatgg tgttcttgcc tttactcgag gaatcgaaat cggacatatc ttcaaaatcg
     4381 gcactcgtta cagtgaatct atgggagcaa ctgtactaga cgaaaatgga cgagaaaaat
     4441 ttgtgattat gggctgttat ggtatcggtg taagtcgttt attatcagca atcgtagaac
     4501 aacattcaga tgaacaagga atcaactggc caaaaggaat tgcgccattt gatttgcata
     4561 tcgtccaaat gaatctaaaa gacgaatacc aaacaaacct gacgaatgaa ctagaagaag
     4621 cgatgacaaa agctggctat gaagtactgg ttgatgacag aaatgaacgc gcaggagtga
     4681 aattcgcgga ttctgatttg attggctgtc caatcagaat cactgtcggt aaaaaagcag
     4741 tggatggaat cgtagaagtg aaaatcaaga aaactggcga aatggttgaa gtacgtaaag
     4801 aagaattggt gaatacttta ccaatcctct tgaaccaagg cgaataataa gcgttaaaac
     4861 acagaaaata aaaatagctg aatcagctgt ttttgacaat ctgatgattc gcttttaaaa
     4921 ttggaactgc gatttgaatc gcagttccaa ttttttaatg taaaactcag aaaaaacgag
     4981 tatactaaaa ggtactgaaa aagaagagga taagatgcga tcaattgcat tgtttctcat
     5041 cagaaagggc gtatatcttt gtcagaaaaa cgagatttat ttgaaaaact gttggaacaa
     5101 atccaactag aggaagcaga aaaaaatcat ccactgctta ctgctggtga aatggatagt
     5161 gtagtggtcc atcgtaaatc gcgtttatgg gaatttacgc tgcatttttc caaaattttg
     5221 cctattatgc tatatcggtc attgactcag catctgacta tcgcttttaa agatattgca
     5281 caagtcaaac tgaatatctt agcagatgac cagacattcg atgaacagct cttgcaagat
     5341 tactgggccc aagctttgga aaatcagcaa tgcgatacac cactagcaca gcaagtattg
     5401 aaaacgcagg tacctgtgat aaaagatcgt aaagtcatct tgccaatcga cagtacagga
     5461 gctatttctt atttgaagca acaatacttg ccattagtag aagagttgtt cgtcagttac
     5521 ggattcccaa aatttcgtat cgaaccagaa gtggatgagc agcaagcgga acgagtattg
     5581 aaactttttg aagaacgcaa acaagaacag gcagaagcct ttatgaagca agcagctgaa
     5641 agtctagttg tccacgaaca aaagaaaaag gaaagaaaag agcaatcgcc agctcttgaa
     5701 ggacccatcc acatccagct aggacgaaat attccagccg atgaaccaac gacaccaatg
     5761 atcagtatcg ttgaagaaga acgaagagtc acacttgagg ggtacgtatt cgacaaagaa
     5821 gtcagagaac tacgttcaaa acgcaagatc ttgactttga aaatcacaga ttatacttca
     5881 tcatttatcg tcaaaaaatt ctcgaacggc gaaaaagatg aacaagtatt tgatgcgatc
     5941 agtgtcggta gctggttaaa ggtacgagga agtattcaag aagatacgtt tgtccgtgat
     6001 ttggtcatga atgcgcaaga tatcatagaa gtcaaacata cacctcgcaa agattatgct
     6061 cctgaagggg aaaaacgagt agagcttcat gttcatagca atatgagtac gatggatgca
     6121 acaaacagca tctctgatct tgtggcacaa gcagggaaat ggggacaccg agctatcgcc
     6181 attacagacc acggtggtgc gcaagccttt ccggaagcac atagtgctgg aaaaaaagca
     6241 ggtgtgaaaa tcttatacgg cgtggaagcg aatgtcgtag atgatggtgt cccaattgct
     6301 tataatgatg cacatgaagc attgagtgaa gccacatatg tcgtatttga cgtggaaacg
     6361 acaggattgt ctgcagtata tgacacgatc atcgagctag ccgcagtcaa aatgtataaa
     6421 ggaaatgtga tcgaaagctt tgatgaattt atcgatccag gacatccttt gtcacgaaca
     6481 acggtagatc tgactggtat cacggatgga atggttcgag gatcaaagtc ggaagaagaa
     6541 gtattgcgga tgttccttga attttcaaaa gatacaattt tggtagccca caacgcggct
     6601 tttgatatgg ggttcttgaa tactagctac gcaaggtatg gtattcctga agcggccaat
     6661 ccagtcattg atacgttgga attagctaga tatctgtatc ctcaattcaa acgtttcgga
     6721 ttaggtgtgt tatctaaaaa atttggtgtc agcttggaac agcatcaccg agcaatctat
     6781 gatgctgaag caactggtca cttagcatgg atctttgtga aagaggcaat ggacaatcat
     6841 aacatgcttt atcatgatca gctaaatgag catatcggag aaggagattc ttacaaaagg
     6901 gctcgacctt tccatgtaac gattttagca aaaaatcagg caggactgaa agatttgttc
     6961 aaactgattt caatgtcgaa tgtcgaatac ttcgaacgcg tcccacgaat accacgctcc
     7021 caattgaaaa agatgcgtga aaatctattg atcggctcag cttgtgataa gggagaaata
     7081 ttcgaagcca tgatgcaaaa aggggtagaa gaggcaagaa accgagcaaa attctacgat
     7141 tatattgaag ttatgccaaa agcggtctat gcgcctttga tcgagcaaga attggtaaaa
     7201 aatgaacatg atttggaaga aattatccaa aatttggtag aaatcggaaa aagtttggat
     7261 aaaatcgttg ttgcaacagg taatgtacat tatctgaacg aagaagatgc catttaccga
     7321 aaaattttga tcaattctat gggcggagcg aatccgttga atcgtcatag tttgccggat
     7381 gtccatttcc gaacaaccga tgagatgcta actgcatttc actttttagg ggaagaaaca
     7441 gcgaaagaga tcgtagtaga aaatacgaat aaaatcgcag atatctgcga agaagtgatt
     7501 ccagtcaaag atgaacttta tacaccgaag attccaggtt cagaagatga aatatcagaa
     7561 ttgagttaca caaaagcaaa acaaatgtac ggagatccgt tacctgagat cattcaaaaa
     7621 cgattaaaaa aagagttgaa ttctattaat ggtaatggct tctcggttat ttatctgatc
     7681 gcccaaaaac tagtgcataa aagtaatgaa gatggctatc tagtaggttc acgtggctct
     7741 gttggctcta gctttgttgc gacgatgaca ggaatcactg aagttaatcc gttagcccct
     7801 cattattatt gtccagagtg ccaatattcc gagttttttg aagatggtac gtatggttct
     7861 ggatttgaca tgccagaaaa acaatgtcca aaatgtggtg cacgcttaaa caaagacggt
     7921 catgatattc catttgagac atttttagga ttccatggag acaaagtgcc cgatattgat
     7981 ttgaacttct caggtgatta tcaagcagaa gcccataact atacgaaagt attgtttggc
     8041 gaagactatg tttaccgtgc cggaacgatt ggtacagtcg ccgacaaaac agcatatggc
     8101 tatgtaaaag gatacgaaag ggacaataat ctgcagtttc gaagtgcaga agtcgacaga
     8161 ttagcaaaag gcgctactgg agtcaaacgg acaaccggcc agcatccagg ggggatcatc
     8221 gttattcccg attatatgga tgtctatgat tttacgccga tccaatatcc agcagacgat
     8281 caaaattcgg aatggaaaac gactcatttt gattttcact cgattcacga taatgtattg
     8341 aaattagata ttctagggca tgatgaccca actgttattc ggatgcttca agatttatca
     8401 gggatcgatc cacaaacgat ccctacggat gacccggaag tgatgcggat atttgccggt
     8461 cctgaagtac ttggagtaag tcaggaacaa atttattcca aaacagggac attaggtatt
     8521 ccagaattcg gcactcgttt tgttcgaggg atgctagaag aaacgcatcc tacaactttt
     8581 gcagaactac tgcaaatttc cggactatct catggtacag acgtatggtt aggaaatgcg
     8641 gaagaattga ttcgacgagg agatgcgaca ctggcggaag taatcggctg tcgtgatgat
     8701 attatggttt atttgatcca tgctggatta gatagcggga tggcatttaa aatcatggaa
     8761 actgttcgta aaggacaatg gaacaagatc cctgatgaat tgcgagaaac ctatctatcg
     8821 gcgatgaaag aaaataatgt gcccgactgg tatatcgatt cttgttcaaa aatcaaatat
     8881 atgtttccga aagcccatgc tgcagcttac gtattgatgg ctcttcgtgt cgcttatttc
     8941 aaggtttatt tccctatttt gtattactgt gcatactttt ctgtacgtgc agacgacttt
     9001 gatctagtct ccatgtgcaa aggaaaagat gctgtgaaac aagcaatgaa agaaatcacg
     9061 gacaaaggat tagatgcttc cgtaaaagaa aagaatcaac tgacagtgct ggagttagcc
     9121 aatgaaatgc tggaacgagg attcaaattt ggaatgattg atttgtataa atcggatgcc
     9181 gttaactttg ttattgaagg tgatacgttg atcgcaccat ttagagctgt cccaagtttg
     9241 ggaaccaacg tggcaaaaca aatcgttgaa gcaagaaaag acggtccatt tttatccaaa
     9301 gaagatcttg ccacccgcgg taaagtttca aaaacattga tcgaatatat gaacgacaat
     9361 ggcgtattga aagatctacc agatgaaaat caattatcgt tattcgatat gctataagca
     9421 tctgttttcc tgtctgcttg taattttaag cagagtatgg tatagttctt ataggtaatg
     9481 atactaactg tgagtgagcg gatttttcgc tcactctttt taatggaatt acgcacttaa
     9541 ttttttaagg aggcgaaact tttgagtagc gtcgttgaaa cagtcacaga aatggtgaca
     9601 ccgattttag atgaacaaaa ttttgaactt gtcgaagtcg aatttgtaaa agaaggaaaa
     9661 agctggttct tacgagtatt tattgacaaa gaaggcggta tcgatatcga agaatgcgcc
     9721 tttgtcagtg agaaactgag tgaaaaactt gatgcagcag atccagatcc gattcctcaa
     9781 gcttatttcc tggaggtatc ctccccggga gcagagcggc cattgaaaaa agaaagcgac
     9841 tatgaaaagg cacttggtga ttatgtccat gtctccttat accaaccagt agatggcgaa
     9901 aaacaatatg aaggatttct tcaatcattt gatgctgaac agcttacatt gaaaatacgc
     9961 ataaaaacac gagaaaaaga attgactttt gaccgaaaaa atattgcaaa agctcgctta
    10021 gctatccaat tttaatcacg gagaggaatc agaaaaaatg agtaaagaaa tgttaaatgc
    10081 gttagacgct ttagaagctg aaaaagggat ctcaaaagaa atcgtgatcg atgctttaga
    10141 agcagcattg gtttctgcat acaaacgtca ctatggccaa gcacaaaacg tcgaagtgga
    10201 atttgatcag aaaaaaggaa agatccatgt ctatgcggta aaagaagtaa cggaagaagt
    10261 gatggattca cagttggaag tctcattgaa agatgcactt ttaatcaatc cagcatatga
    10321 gatcggtgat acgatccgtt ttgaagtaac accaaaagat tttgggcgga tcgctgcgca
    10381 aacggctaaa caagttattt tacagcgtgt acgtgaagca gaaagaacga ttatttataa
    10441 cgaattcagt gcttatgaaa aagacatcat gcaaggaatc gtcgaacgcc aggataaacg
    10501 ttatatttat gtcaatcttg gtaaaattga ggcagttctt tctaaacaag atcaaatgcc
    10561 aaatgaattt tatcaaccac atgatcgaat caaagtttat gtttcccgag tagaaaatac
    10621 gtctaaaggt ccgcaagtat ttgttagccg cagccatcca gacctattgc gccgcttatt
    10681 tgagcaagaa gtaccagaag tctatgatgg aatcgttgaa attgtcagca tcgcccgtga
    10741 agctggcgat cgttcaaaag tggcagttcg ctcaaatgat ccaaatatcg atgcagtcgg
    10801 tacatgtgtc gggccaaagg gacaacgagt tcaagcaatc gtcaatgaat taaaaggcga
    10861 aaacatggat atcgtcgaat ggaatgaaga tccagctgta ttcattgcaa atgcattgaa
    10921 tcctgcacaa gtcatggatg tgatcttcga tgagacaaat ccaaaggcct gcacagttgt
    10981 cgttccagac taccaattat cgctagcaat cggtaaacgc ggacaaaacg cacgtttagc
    11041 tgctaaattg actaaccata aaatcgatat caaatcagaa tctgacatga cggaatttta
    11101 tgaaaatcaa gaacaacaaa aagaaacaga agaactgcat gatgaagcaa tcgttcagtc
    11161 agatttaaca gacgatgaat atgaaacaat tgcattcaac aatgaaacgg tcgaagaaaa
    11221 accagaagct taactttagt aaggaggcaa agagatgaaa caaaggaaaa ttcctttacg
    11281 caaatccgtg gtttcaggtg aaatgaaacc taaaaaagag atggtccgaa tcacccgttc
    11341 aaaagaaggg gaagtatcga tcgatccaac tggaaaaatg cctggacgag gagcgtatgt
    11401 ctcacttgag ccggaagaag tacaacaagc ttgggataaa aaaattctcg atcgagtact
    11461 cgaaacaaca ttaacagatg agttttatca agaacttctt gactatgtca ctcaccaaaa
    11521 agcacgtaaa gagttatttg gaaaatgaat acagttaatc ggcaaaaagc catgaatctc
    11581 atcggtctag cgatgcgcgc gggaaaacta atcacgggtg aagaactgac cattgctgaa
    11641 atccgcaaac aaaaagcaaa attggtgttt gtggcaaccg atgcaagcga gaatacaaga
    11701 aaaaagataa aagataagag ttcatattat gaagttcctt gctttgagct attttctgaa
    11761 gcagaaatca cgcagatgat cggaaaacct cgcaaggtat tcggaataat ggatgccgga
    11821 tttgcgaaga agaccaagga gctaattgaa ggttaggaag gtgattgcat gggcaacaaa
    11881 agaatctatg aattagcaaa agaactcaaa aaatctagta aagacgtcgt ggaaaaagca
    11941 caacaacttg gcatcgatgt gaaaaaccat atgggtgcga ttactagcaa tgatgagaag
    12001 aaattgcaac aagcgttcaa aaacccaagc acgaaacaac aagctgcaca acaagcaagc
    12061 agaaaaccag ccaatcaaca gccgaacgat caacgtaaaa aagaaacaac gaacaatcaa
    12121 caaaaaaata aaaataaccg cagttatcaa gatcgcggtc aagggagcgg tcaagtgaat
    12181 caaggaaaaa aaccaacaac aaaccaaaat aatacgggaa ataccagcag ccaaaatcgt
    12241 caaggaacga gccaaggcgg caatcaaaac cgtactaaca ataacaacaa accaaacaat
    12301 aacaatcgcg gcaaatttaa taataataac cgtaatcgtt ataataaaaa aggtaaaaaa
    12361 ggcaaacagc aacaatcaac taaaccagct gtaccaccac gcaaattccg tgaattgcca
    12421 gatgtattag agtatactga aggtatgaac gtagcagata ttgcgaaaaa aattcatcgt
    12481 gagccagcag aaatcattaa aaaattattt atgcttggtg tcatggtcaa tcaaaaccaa
    12541 gcactggata aagatacgat tgaactgtta gcgacggact atggcatgga accacaagaa
    12601 aaagtccaag ttgacattgc tgatatcgat aaattcttcg aggcagaaga agtcgatcca
    12661 gataaattag tctcacgtcc gccagtcgta acgattatgg gacacgtaga ccatgggaaa
    12721 acaactttat tggatacatt acgccattca cgtgtaacaa gcggcgaagc cggcggaatc
    12781 acacagcata tcggtgccta ccagatcgat attgatggta aaccaatcac tttcttggat
    12841 acaccaggac atgcggcctt tacaagtatg cgtgcacgtg gagcaagtat cacagatatc
    12901 acgatcttag ttgttgctgc agatgatggc gtcatgccac aaacagttga agcaatcaac
    12961 catgccaaag ctgcaggcgt tccaatcatc gtcgctgtaa ataaaattga taaaccaggt
    13021 gcaaaccctc aacatgttat gcaagaatta agtgagtatg agttaattcc tgaagcatgg
    13081 ggtggagaaa cgatctttgt tgagatctca gcgaaattcg gccaaaacat cgaagaattg
    13141 ctggaaatga tcctgttagt cgcagaagta gaagatttaa aagcagatcc aacacaacgc
    13201 gcaatcggaa cagtgatcga agcacgtctt gataaaggta aaggtcctgt gtcaacctta
    13261 cttgtccaag aaggaacact tcatgttggt gacccaatcg ttgtcggtaa tacttacgga
    13321 cgtgtccgtg taatgacaaa cgatcttggc cgtcgcgata aagaagcagg acctgcaacg
    13381 ccagtagaaa tcactggctt gaatgatgta cctcaagcag gtgaccgttt cgtagtcttc
    13441 gaagacgaaa agacagcacg tgcagcgggt gaagaacgcg cgaaacgtgc attattagaa
    13501 aaccgtgctg ccacaagccg tgtgactttg gataacttat ttgaaagcct gaaagaaggc
    13561 gaattgaaag aagttaacgt aatcatcaaa gcagatgtac aaggttcagc agaagcactg
    13621 gctgcatcat tgaagaagat tgatgtggaa ggtgtccgtg taaaaatcgt ccattcagct
    13681 gtcggagcaa tcaatgaaag tgatgtaacc ttagcagcag ccagcaacgc tattatcatc
    13741 ggtttcaacg ttcgtcctac accgcaggca aaagtgcaag ctgatactga agaagtcgat
    13801 attcgtcttc accgtatcat ctataaagcc atcgaagaaa tcgaaactgc gatgaaaggg
    13861 atgctagacc ctgaatttga agaaaaaatc accggtcaaa tgatcgttcg tgaaaccttc
    13921 aaagtttcta aagttggtac gattgcaggt gcctatgtaa cagaaggata tattcgtaga
    13981 gatagcggtg tccgtgtaat ccgtgacgga atcgtaatct atgaaggcca acttgcaagt
    14041 ctgaaacgat tcaaagatga cgtgaaagaa gtcaaaatgg gttatgaatg tggtgcaatg
    14101 attgaaaaat tcaatgacat caaagtagat gacgtaattg aaggatttat catggaagaa
    14161 gtcaaaaatg actaaaaact gataaaagga ggccaatcat tatggctaac taccgtgacc
    14221 gccgagtcgg tcaagaaatc ttaaaagaag tcaatgatat tttgcgcaaa aaggtccgtg
    14281 accctcgtgt ggagaacgta acgatcacag atgtgaaagt tacgggggac ttacagcaag
    14341 caacgatcta ttacagcatt ttatctgact tagcatcaga taagaaaaca gcacaagatg
    14401 gattgaataa agcatcagga ttgattcgtc gagagttagg gcacaatcta agtatttata
    14461 agacacctga attgttcttt gaacttgatg aatcagttgt ctatggcaac catatcgatg
    14521 aattgcttcg cgacttgaac aaagactaat caaaaactcc cgcaaatcct gtgaaatcag
    14581 gggttgcggg atttttttgt tttcattaag acgtattacc taagagttcc atgactttag
    14641 tcttgcggcg tgtcagctaa ccaaaaaaga gaaaaagaaa agtaaaaaat tcgactttct
    14701 tttttctagt agtatataaa tttttgcata tagataaaga tcaagatacc gataacggaa
    14761 gctagattgt aatctatttt agtgacagtc aggtatagcc aagtaactag aagatcagtg
    14821 gaacctgcac cataaaagtt tgagtttgtc ggtccgttga cagttaaaag aaaaatcacg
    14881 ttgaagttat tgatatttcc gattacgccg cttcgatttg gtcacgatcc aaattttgga
    14941 taatccctgt agaaattagc atagtagctg ggataccaat tcacatattg acaaagataa
    15001 cagagacttt tacataaaac ggatcaatca ggaaaggaat tgctcgatcc agccccagtt
    15061 ttccagcaat gtgttaactg gcccagcgtc attcagcaaa ttatgcatga tgaggagcga
    15121 aatgaactgc ggaacagcca ttgtgataac gacaagtttt ttggatcaat gtaaaaaacg
    15181 tcagcgaaag gaaaaatttc gtggtttttt atacctgttc atttggctag atataaaatc
    15241 caaacaaatg aacagaagtg cttattaggt ttcaattggg agtatctgcg ctagaataag
    15301 agtaataatg aaaaggagtg ttatgtatgg attattctag taaagaatac tttgataaaa
    15361 tgacggcttg gtggcgtgct gctaattatc tttctgttgg gcaactttat cttaaagata
    15421 accccttact gagacgtaca ctgaaacccg aagatgtcaa aaaacatcca atcggtcatt
    15481 ggggaacgat tcctggccaa aattttattt atgtccattt gaaccgagtc atcaataaat
    15541 atgatttgaa catgttttat attgaagggc caggacacgg cggacaagtc atggtatcga
    15601 atgcttattt agatggaagt tacacagaga tctatccaga agtaacggaa gatgagacag
    15661 gaatgcaaaa attattcaaa cgattttcat ttcctggagg cattgcttct catgcagcac
    15721 cagaaacacc aggttctatc catgaaggcg gagagcttgg atactctctt tcacatgcag
    15781 tcggagctgt attagacaat cctgaagtaa tctctgctgt cgtgattggt gatggtgaag
    15841 cagaaaccgg tcctttagct ggaagctggt tctcaaatgt ctttatcaat ccagtgatag
    15901 atggagctgt tttaccgatc cttcatttaa atggtgcaaa aatagccaac ccaacgatct
    15961 tggcacgtaa atcagatgga gaattagcaa attactttaa cggacttggc tgggaacctt
    16021 tctttattga aggaaatgat tcagaaaaat taaatcctgt tatggctgaa aaaatggatc
    16081 aagcaatcga gaaaattaaa tccatccaaa aagaagctcg cctgaagaca gcaacagatg
    16141 tcgtgatgcc aaaatggcca gtattgatcg tacgcacacc aaaaggatgg accggtccaa
    16201 aagaatggga cggggaacca attgaaggaa ccttccgagc acaccaagta ccgattcctg
    16261 tagatcaaga acatatggat catgcagatg ctttactccg ttggctgaaa tcctatgaac
    16321 cagaaaaatt atttgatgca caaggacgta ttttagaaga aatcagagaa atcgcgccta
    16381 ctggtgacca aagaatggca aaaaatccta tcaccaatgg tggaatcgat ccaagaccgc
    16441 ttattatgcc agattggaaa aaatatactt tgcaatttga aaaaccaggc tccgttaaag
    16501 cagaagatat gaccgaactt ggtaaattcg ttcgcgagat cattgagaaa aatccagaaa
    16561 atttccgtat atttggacca gatgaaacaa aatcaaaccg attgaatcaa gttttcaaaa
    16621 cgacaaatcg ccaatggatg gaaaaaatcg aaccagaaaa tgatgaatgg ctttcgccat
    16681 ctggtcgcgt catcgacagt caattatcgg aacaccaaga tgaagggttc ttagaagggt
    16741 acgttctgac tggacggcat ggttttttgc aagttacgaa tcattcttgc gagtggttga
    16801 ctctatgttg actcagcact tcaaatggat gcggaaatca catgaccttt cttggcgaaa
    16861 tgattatcct tcgctaaatc tcattgcaag ctcaacagtc ttccagcaag atcacaatgg
    16921 atatagccac caagatccag gaatattgac acatttggca gaaaagaagg cggagttcat
    16981 ccgtgaatat ctgcctgcgg atgcaaatac attgctcgca gtcatggata aagcattccg
    17041 ctcgtcagaa aaaatcaatt tgatcatctc ttccaaacat ccgcgtgctc aattttactc
    17101 agcagaagaa gcagccgtct tagtgaatga aggcttgaaa atcattgatt gggcttcgac
    17161 agcaaaagag gaggaacctg aattggtcat tgctgctgca ggaacggaat ctaacttaga
    17221 agcattagca gctgtaactt tgctgctgga agaattccca aaactaaaaa ttcggttcat
    17281 caatgtcgta gatttattga aattacgtca tccaagtcaa gatccgcgtg ggttgagcga
    17341 tgaagaattt gatcaatatt ttacaaaaga caaaccgatc ttatttgctt tccacggtta
    17401 tgaaacattg gtccgcacga ttttctttga tcgtcataac catcatttaa tgattcatgg
    17461 ctacaaagaa aatggagata tcacgacacc atttgacatg cgtgtagtaa atgaacttga
    17521 tcgttatcat ttggcaaaag acgcagcttt aaaaatcaaa ggaagtcaag cagaagattt
    17581 tgcgaaaaaa atggatcaga aattacaaga acatcaaaac tatattcgtg aaaatggtat
    17641 tgatctgcca gaagtattgg attggaaatg gaaaaatctt gaccaataaa atgataaaat
    17701 cgatcccgca aaatagaatt ttttacattg attaagaaac tgaaaaaata aataaaaaaa
    17761 acggaaaaaa gactggaaga atggccagtc ttttttttcg tttttgggaa gtgaaaacca
    17821 aactatttac ctgtaagcca cccataaatc caatcaaatg gtgcaaagaa aaaatcttcc
    17881 attctattcc ctcctttaaa aaagcataac atgatttcta tttacgtgag catgaatttt
    17941 gttacgtttt tattgccgaa aaataaaaac cgttcattat gatgtcaaat tcgtcagtaa
    18001 ttgatgcaat gtgttataat aattcagtta aatcaaaaat gaaatggaga aaagacatgg
    18061 acgggttatt acctttatgg aaagaacgcg gaatgacaag tcatgattgc gtattcaaat
    18121 taagaaaaat cttacatacg aaaaaaatcg gacatggggg tacgcttgac ccagacgtag
    18181 acggtgtgtt gcccatttgt attggaaaag ccacaaaagt tatcgaatat ttgacagatt
    18241 ctgggaaaat ctatgtaggc gagataactc taggtttcag tacaacgacg gaagatcgat
    18301 ccggagaacg ggtagaaaca aaaccagtag atatgtcttt atctgaaaaa aaaatagatg
    18361 aagtgatggc ttcatttatt ggtgagatca cccagatacc accaatgtac tcagcggtaa
    18421 aagtcaacgg taagcggtta tatgaatatg cccgcaataa tgaaacagtg gagcgtcctg
    18481 ttagaaaggc tcagatttac cgttttatcc ggacaagtga attgacaaga gacgaagtga
    18541 caggaaccat ttcttggcgc ttcgaagtag aatgcgggaa aggaacctat gtccgcacat
    18601 tagctgtaga tacaggcaag aaacttggtt accctgcgca catgtctgat ctgacccgaa
    18661 cagcaagtgc cggtatgtcc cagaaggatg ctctgacatt agaagaagta gctcaagctg
    18721 tgaaaaatgg gacaatcgat gattgtttgc tgccaataga aaaaggagtg gaacggttca
    18781 aacgagtaga tattactgat gaagtatggc agaaagtaaa aaacggtatg agacttgatt
    18841 atactgtatt tgcgctccca gaaatgccgt cagaagaaat tgctttgttt tataaaggaa
    18901 aagttgtcag tatttatcag ccaaacccga aagaaaaaaa caaattaaaa cctagtaaag
    18961 tattgagaaa tgaggaataa cgaatgaata tcatcaagat cagacatcct taccaatcag
    19021 aacaaattcc agaagacgat gtcgttttag tgctgggatt tttcgacggc gtgcatatcg
    19081 gacaccagaa agtcataaag acaggtatgg aaattgcaca aaaagaagga ttgaaattag
    19141 cattgatgac tttcaatcag catccatcta ttgtctttaa aaagatcgat cctagcagcg
    19201 tgaaatattt gactagcctt aagcaaaaag aagaaaaaat ggccgctttg ggtatagatt
    19261 atttatatga agttgatttt acttcttctt ttgctcattt agcaccgcag gaatttgttg
    19321 atcaatacat cgtagggtta catgcaaaat atgctgtttc cggttttgat tatacgtatg
    19381 gaccaaaaga cattgcagat gtcgctcatt ttcctgaata tgccaaagga agattcaaaa
    19441 tcgtcacagt ttctaaagaa gaagaacaag gtgaaaaaat cagctctacc cgtattcgtg
    19501 gattactaga cagcggacaa gtaaaagaag cagcaaaact attaggttgc acatatgagg
    19561 tagaaggggt agttgtccat ggcgatgcac gaggcagact actaggattc cctacagcaa
    19621 atgtcaaagt aaagagcacg gttcatttgc ctaaagaagg tgtttatgtg tcggaaataa
    19681 aaattgggga aacatggtat ccagccatgg gttcaattgg acataatgat acattcggtg
    19741 aaggacggga gctgactgta gagttgtata ttttggactt taatcaagat atctatggtg
    19801 aacatgtcgc ggtacgctgg aatcaatttt taagagatca agtgaaattt tctagtgtag
    19861 aggaattgat tgaacaactc aaggaggatg aacaagcaac agctgaatat ttttctcagc
    19921 aataaaacat tatgcataaa atctcttgtt tatgtataac tagcaattac aataaaatgg
    19981 gtgataaata agcccaggaa aggggctgtg acaaaagtct tgtcacagcc tttttttcca
    20041 aataaatggt ttttcagaag tagcttcttc ggaaataagc cgaaatttct tgaagctgac
    20101 cacttctgtc acaacctctt tctttataga aataaaagga cactagcaat tcatgaaaac
    20161 gattgctaga gcgtatcatt tgctttagac tatatatctt ggtgtacact cactttaagg
    20221 cggaaataaa agttatccgt aataacaggt ttaggtatga aaaaggagtg ggtactatgt
    20281 acaaggaatt agaaaacaaa gtcgcagtag taactggtgg ctcaaagggg attggaacag
    20341 cgattgctca acgattcggg aaagaaaaaa tgaatgtagt tgtcaactat aactcggata
    20401 aagaaggagc agaaaaagca gtcaaagcaa tcgaagaggc aggcggaaaa gcagtctctg
    20461 tacaagcaga tgtcagttct gaagaaggaa tacagcgttt acttgatacg gctttagaaa
    20521 attttgggac actggacgtt tgggttaata atgctgggat ggaaaatcag catccaaccc
    20581 atgaacttag ccttgaagat tgggaaagag tgttaaaagt caatttgact ggcgttttta
    20641 tcggaacgaa agcggcttta aattacttta cagagaataa taaaaaagga aatatcatca
    20701 atatgtcttc tgttcatgaa cagatcccat ggccaacgtt tgctcattac gctgcttcaa
    20761 aaggcggggt aaaattattt accgaaacgg tagcaatgga gtatgcaccg aagggaatca
    20821 gaatcaatag tataggccct ggtgctatcc gtacacctat taatgcggca aaatttgatg
    20881 atcctgaaca aaaagaactc ttggaaagta tgatcccact aggacgcgta ggtgaaccaa
    20941 aagaaatagc ggcgacagct gcatggttgg cttcagacga atccagttat gttacaggta
    21001 ttacactatt tgcagatggt gggatgactt tatatccttc tttccaaggt ggaaaaggat
    21061 aagggtgaat aaaaatgggt ttattgattg cattgatccc ggcagttggc tgggggatcc
    21121 agccactgat tttgaaaaag attggtggca gaccaacaaa tgagatatta ggaactggta
    21181 ttggagcggt gcttgtcggc ttactcgttc aattattcgc ttctccagct ggtatttcag
    21241 tgactacatt tttgattagt ttactttctg gtgcattttg ggttattgga caaacaggac
    21301 agtatacatc tttcaacttg attggtgttt ctaaaacgat gccaatttcg actgcattac
    21361 agttagtcgg aacctcgttg attggtgtct tcgcttttgg agaatggtct ggaagtatgg
    21421 ggaaaatcat tggagcagca gcaatcattc ttcttgtatt tggttctgct ttaacggcaa
    21481 tcagtgatga tcctggaaaa aaaggcggta tcacaaaagg aattagtcta ttagcatcaa
    21541 caagtattgg ttattgggta tacagtgcat tgcctaaact agttgatgca agcggacttt
    21601 ccattttctt tccgcaaatg atcggtgtct ttttaggggc agtgatttat gtgattttcc
    21661 gtaaaccaac cgcattttct gaagacaaga gttggaaagc aacgatcgtt ggagccatct
    21721 ttagtgtgtc agcattggct tatatttttt cagcaaatga aaatggggtt gctactgcat
    21781 atatcattac acagctaaac gtagttatct ctacattagg cggtatgtac atcctgcatg
    21841 aaaataagac accaaaagaa aaaaaattaa cgattatcgg attattgctg atcgtagtag
    21901 gaagcatggt cacggtcttt atttgaggat aaagatggta aaatttcatt tattaaaaaa
    21961 atatttgttt gaaccaaaca gtaagtactt ataaaaaaat agcgctagaa tttccaacat
    22021 ctctggaatt tctagcgctt tttaattgtt taagatacaa gggaaatatc tgtctggatc
    22081 aataattttt cattgatcca ttgactaaag tttacgacat aattgaagtt accaccgatt
    22141 agtatctccg catgattgtc tttataaaga tcgtattgta aaagacggtc ttttttattt
    22201 gcgtaaacat cgttatggta ctggactaat tgttctcgga attgattgat tgctgtaaaa
    22261 tcacgatatt gaaggttgaa acgcatcgtg attggtgcat ttagcatgaa tcgctgcatt
    22321 tcttttgctg cttcctctgc ttgatctccg tataggaact caaaaagatt acgttcagaa
    22381 aatttccatt gcccgccaac ctttgttcct tttagttttc cttcatgaag ataatttctg
    22441 atagaacgtg tagtaacacc taattgttcg gctaattcat ttactgagta aaaatctttc
    22501 aatgttcacc ctcctatgtg aatggttcta ctgacttagt tgatatttgc ttaatcacga
    22561 aatgtagaaa gaacgatttt aattaaggtc attgtaaaaa tcaagtcaga aaattctaac
    22621 ttttatttct ctaaagaaat t
//
LOCUS       DAZULY010000016        22062 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000016 DAZULY010000000
VERSION     DAZULY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.016"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            196..1902
                     /gene="efbA"
                     /locus_tag="ACG81A_000442"
     CDS             196..1902
                     /gene="efbA"
                     /locus_tag="ACG81A_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFS5013837.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2060..2962)
                     /locus_tag="ACG81A_000443"
     CDS             complement(2060..2962)
                     /locus_tag="ACG81A_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5013838.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2959..3651)
                     /locus_tag="ACG81A_000444"
     CDS             complement(2959..3651)
                     /locus_tag="ACG81A_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFS5013839.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3694..4335)
                     /locus_tag="ACG81A_000445"
     CDS             complement(3694..4335)
                     /locus_tag="ACG81A_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFS5013840.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4373..4651)
                     /locus_tag="ACG81A_000446"
     CDS             complement(4373..4651)
                     /locus_tag="ACG81A_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013841.1"
                     /translation="MRFVSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFI
                     FLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            complement(4678..5304)
                     /gene="pyrE"
                     /locus_tag="ACG81A_000447"
                     /pseudo
     CDS             complement(4678..5304)
                     /gene="pyrE"
                     /locus_tag="ACG81A_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5311..6027)
                     /gene="pyrF"
                     /locus_tag="ACG81A_000448"
     CDS             complement(5311..6027)
                     /gene="pyrF"
                     /locus_tag="ACG81A_000448"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFS5013842.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(6024..6956)
                     /locus_tag="ACG81A_000449"
     CDS             complement(6024..6956)
                     /locus_tag="ACG81A_000449"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFS5013843.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6953..7729)
                     /locus_tag="ACG81A_000450"
     CDS             complement(6953..7729)
                     /locus_tag="ACG81A_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFS5013844.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7783..10968)
                     /gene="carB"
                     /locus_tag="ACG81A_000451"
     CDS             complement(7783..10968)
                     /gene="carB"
                     /locus_tag="ACG81A_000451"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFS5013845.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10973..12049)
                     /locus_tag="ACG81A_000452"
     CDS             complement(10973..12049)
                     /locus_tag="ACG81A_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFS5013846.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12054..13334)
                     /locus_tag="ACG81A_000453"
     CDS             complement(12054..13334)
                     /locus_tag="ACG81A_000453"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFS5013847.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13353..14279)
                     /locus_tag="ACG81A_000454"
     CDS             complement(13353..14279)
                     /locus_tag="ACG81A_000454"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFS5013848.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14292..15575)
                     /locus_tag="ACG81A_000455"
     CDS             complement(14292..15575)
                     /locus_tag="ACG81A_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFS5013849.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15589..16128)
                     /gene="pyrR"
                     /locus_tag="ACG81A_000456"
     CDS             complement(15589..16128)
                     /gene="pyrR"
                     /locus_tag="ACG81A_000456"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFS5013850.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16363..17274)
                     /locus_tag="ACG81A_000457"
     CDS             complement(16363..17274)
                     /locus_tag="ACG81A_000457"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS5013851.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17271..17735)
                     /gene="lspA"
                     /locus_tag="ACG81A_000458"
     CDS             complement(17271..17735)
                     /gene="lspA"
                     /locus_tag="ACG81A_000458"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFS5013852.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17740..18438)
                     /locus_tag="ACG81A_000459"
     CDS             complement(17740..18438)
                     /locus_tag="ACG81A_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFS5013853.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18425..18811)
                     /locus_tag="ACG81A_000460"
     CDS             complement(18425..18811)
                     /locus_tag="ACG81A_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013854.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18958..20628)
                     /locus_tag="ACG81A_000461"
     CDS             complement(18958..20628)
                     /locus_tag="ACG81A_000461"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFS5013855.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20811..21044
                     /locus_tag="ACG81A_000462"
     CDS             20811..21044
                     /locus_tag="ACG81A_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5013856.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21143..21574)
                     /locus_tag="ACG81A_000463"
     CDS             complement(21143..21574)
                     /locus_tag="ACG81A_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFS5013857.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21653..>22062
                     /locus_tag="ACG81A_000464"
     CDS             21653..>22062
                     /locus_tag="ACG81A_000464"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFS5013858.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
BASE COUNT         6236 a         4932 c         3896 g         6998 t
ORIGIN      
        1 ggctttttta tttgtctcgc ttaattatac aaacggcgat acctaaaaat ttggatttca
       61 acgatagaaa cactgttttt tatgtaatta gtccaagatt tttctagact tttgctttac
      121 tattcggcca aattggtttt tcttcttttt ttcgttataa tgaatatact tatgaaaaga
      181 aatgaggaat gaaccatgtc ctttgatggt gtatttaccc acgcgatggt caacgaattg
      241 cgtgaaacgt tactatctgg ccggatctcg aaaatccatc agccttatga aaatgaaatc
      301 gtgctggtga ttcgttcacg cggcaaaaac catcgattat tattatctgc gcacccaagt
      361 tatgctcgtg tacagattac ccaaatcgat tatcagaatc ctgataatcc gcctaacttc
      421 gtgatgatgc ttcgaaaata tttggatggc gctattttag aagacatcga acaaatcgaa
      481 aatgatcgag tgatccattt ccactttgct aaacgaaacg agttgggtga tctacaaaat
      541 atcatattga tcgttgaatt aatgggacgt catagtacga tcgtcttagt taatcgagaa
      601 actggaaaaa tactggatgc gatcaaacac attggaagct ctcaaaacac ttatcgttct
      661 ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag aggtcttgaa cccatttagc
      721 tccgagaaag aaaaaatctt tcagcggctt tcccaaactg aactagatcc aaaagcaatc
      781 caacgtcagt ttcagggaat cggtttcgat atcgctcaag agctgaccaa aagattactg
      841 gaacgtccta atgaaaagat ggttgtatgg gacgaatttt tctcagccat cagccatcag
      901 cctattccta ctttttacga aacggaaaat aaagattttt tcacaccgat tgcttatcag
      961 gtattgtccg aacaggcatc tgctgtaaca gcttatccaa cactcagtca attattagat
     1021 agctattatc acgaaaaagc agagaaagat cgggcaaaac aacaaggcgg agaactgatt
     1081 cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat tgaaaaaacg tgaacaaaca
     1141 ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg gcgaactact tactactttt
     1201 ctgacacaag taccacgcgg cgcaaaagaa gttgttttac ctaattatta tgaagaagat
     1261 cggccaatca aaatagcact agatccggca ttgacaccaa atcaaaatgc acagaaatat
     1321 ttccatcgat accaaaagct gaagaacgct gttaagctga ttggcgaaca aatccaagaa
     1381 gccaaagatg aaatccaata tcttgaatct gtcttatctc aactggaaat tgccggccca
     1441 atggatatcg aagcaattaa agaagaattg acttcagaag gatacctgaa aaagaagaac
     1501 ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc aatacttttc ttctgacgga
     1561 acgctcattc ttgttggtaa aaacaatctt caaaatgacc agctgacaat gaagacagct
     1621 aaaaaaaccg actattggct tcatgcaaaa aatatacccg gttcgcacgt catcattaaa
     1681 agtgacaagc cctcagatga aacaattaca gaagctgccg agttagctgc ctatttttca
     1741 aaataccgat actctgccca agtgccggtt gatcttgtac aggtaaagca tatccgaaaa
     1801 ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc aaaagacgat tatcgtgaca
     1861 ccagaagaag aaaaaattac agctatgaaa caaaacaggt aatcaatgag tgtttctaga
     1921 atcatatttg cgtactttcc cacaacagca attagttata aattgtttgc tacaaaaaga
     1981 ggagttgtga caaaattctg tcacaacccc tcttttttga tcaacggtgt tcaataaaca
     2041 gcgttctcat ctgcttttct tagctctgct ggtccagtaa ccgagtaagc tctttgataa
     2101 atctttgagc gatttcactg agcggacgtt tcgccgaata aatgatataa aattcattta
     2161 gttcgccttc tgccaaaggg attaaccgca agtgctcgcg aatccctcca atcacaatac
     2221 cagatcctga agcgtaagca tcagtttcaa gaacgatatt catcaaagta cctcgatcac
     2281 tcgtataaat cactgtttct tgatcaggga tctcgataag gtcttcataa aaatacggga
     2341 aattatttcc atcctgtgta aaacgaactt gaggataaga taacaattct tctttggtga
     2401 tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa aatatgcgtt ttgaattttc
     2461 caatgctgcg gccacttagc tctccttgtt gtaaatatct tctgatacca gcttgattat
     2521 gctcattcat ataaataatg ccgatctcac tgtgctgctc tttgacttct tcgatcactc
     2581 tcgctgttgt cgtctccatg atccgaaatt gtttgatctc gtctttgaat tcctcgagta
     2641 gctgtgcggt caaaggacct aagaaatcat aatgctgggc agcgatcgaa aaattgtctc
     2701 tcttttcact cgttagatag tgagtttcta atgtatcaag ctggaccaat attttctcgg
     2761 cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc ccctcgattc gttcgagtaa
     2821 acagcgtgat ccctaactcg gtttctaatt ctttgatacc agctgaaaga cttggctggg
     2881 agacaaacat tttctttgcc gcttctctga aactaccatt gtttgctaca gcgaccacgt
     2941 atttcatctg ctgaatattc attgctcaaa ctcctcgaaa taataaattt ctcttccatt
     3001 caagggctgg attggtcggt tgttcggtcg agcatactga ccatcgaagg ggagaagaaa
     3061 ctcttggttc agttttccta catgatccat tacaaaccat tggattggcc cgctggtagg
     3121 cggagttgtc aatgagccaa tataatggaa atggcttttt tgctccggaa agaacacatc
     3181 cggatttacc caatgctcat gtttttctaa gttccaactt tccccatttt cttgattata
     3241 cagccaacct tcctgtacca ttagctggta catgacaccc acaactagat tctctttttt
     3301 gctgttcata tgaacaaaat gaaattcaat atcttcctgt tttccgttga tgatatgttc
     3361 agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt tctttcttga actctacata
     3421 actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg aattccttgt cagtaaaccg
     3481 ttgcttttga taatgaaagg aaagcgtgtc tccttctatt ttggtttctt cccccttggt
     3541 caaatgaata ggcgactgca atggaaattc cgcacctctc gcaaaccaat cacataaact
     3601 cgcccaattt tccggacctt tgtctccaga atagccccat tcgacatcca tattcttttt
     3661 caactatact tccccctgca tctcatatca tttttatttt tcttctttct ttacaatcgt
     3721 tgagacggtt tctaaaaacg cctcttttcc ataaaattcc tggttcaata gttccggaaa
     3781 tatctcttta aagaaatact gaacagaagg ccgatgttca aagcttttaa tcgtagtcac
     3841 tgcctcaatg aacatctcct tataaacttt ttccgggtta tttttgatga tttcatcaaa
     3901 agattcataa agaaggtcaa ccttatctga aatggctaat agttctcctt ctacggtttc
     3961 atcttttcct tcgaacaacc gacggcgata aacttcttgg aactcagctg gaatctcttg
     4021 gttgatgaat tcgtccgtca tcttttcttc aactgtttgc agcatccctc ttaactcttt
     4081 gcttgcgtat ttcaccggcg ttttgatatc accaataaaa cgctctgtgt agtcatgatt
     4141 caatgatttt tcatagagca gtttccaatc gatcggttgt ccattcgctt cttcaatgtc
     4201 tcctagaact tgcgcaatag atgctacacg atacgaatgt gctgcaactg tatgctctgt
     4261 atatttaaaa aatccaggtg cccttgtgat cgtctctaaa ttgttcaatc ctaaaataaa
     4321 ttcattcaac cccataaaag ttacctccct ttgagatttc tttttccgaa tatcatatca
     4381 ttaaataact gatattcgca acatttccgc tctttaccat ttttgaaaca gctcccttac
     4441 caaaaaatag agaaaatcta tatataatag aaaaaagaga agagattatt ctcttctttc
     4501 tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa aaactgatca cgatagaagt
     4561 ttcttttcat acctgatgaa aagatgcttc tcgcaatcag tttttggatt tttgctattc
     4621 caattgatga ttctggagac acaaatcgga tttctctatc ttcatcaagc gcgcttcaat
     4681 ctgccaattt tcaggatctt ttttccattc ttttagcaga gttaattctt gctcatcgat
     4741 ataacgatct tctaaagctg cttcaatcaa tacagaataa ttggtcaacg tcataagagg
     4801 gatttcggct ttttcgaaat ttgcttttcc ttttggcaac tcatacgtaa agatagccgc
     4861 aacaccaagg atatccgcac cttctctttt tgctgcttca gctgcttcca agacacttcc
     4921 gcctgtagaa atcaaatcct cgatcacaac catcttctga ccttcaaata tacgtccttc
     4981 gatttggtta cctttcccat gatctttcgc cttacttcga atatagacca ttggcaaatc
     5041 cagtatttcc gctacccatg cggcatgagg gattcctgct gttgcagttc cagcaatcac
     5101 ttgtacttca gggtaagctt ctttgatttt gcttgctaat ccttttgcga tctcttttcg
     5161 gacttttgga tagctcatcg tgattcggtt atcacagtaa attgggcttt tgattccact
     5221 tgcccatgta aaaggattac ttggatttaa aaatactgct tcaatttcta ataagtcttt
     5281 tgcgatactt tgttcaacat tcattttagg ttagctccat tctgtttgaa tcgtttgata
     5341 tgctttataa ggattagttg actgagtgat tggtcgacct ataacgataa aagttgagcc
     5401 gatctttttt gcctgagaag gtgtgactac tcttgtctga tctcctgcga ctgttccttc
     5461 tggtcttata cccggagtta agcaaacaaa ttcgtctcct gccatttctt tgatcgcttc
     5521 tacttcccaa gcagaagaaa ccacaccatc taatcctgct tttttggcac agctagcata
     5581 atggatcaca cttttttcta aagaaacttc gattagttga tcggcatgca tttcttcttc
     5641 acttgtagaa gtaagctgag tgatagctag aagtagcgga cgttttccac cttctggagt
     5701 cccctcttct aatcctcgca ttgccgcctc catcatacga ataccgccag cggcatgaac
     5761 acaggtgaga tcaacaccaa gctttgccaa tccccgcatt gctttttcga ccgtattcgg
     5821 aatatcgtga agtttcagat ctaaaaatac attatgacca gcctctttta gataacgtac
     5881 gatttcaggt ccttcttggt agaacaattc cattccgatt ttcacaaaca atttttcttc
     5941 tttcgggaaa aatcgaagaa agtcttctac ctcttgccta gttgaaaaat ctaatgcaat
     6001 aattggtcgt tggtctagtt gattcatcgt tcacgttcct ctctcacttc tttgattaat
     6061 ctttccagcg attcgatgcc tagttcgctc attcgcacag ggagctcttt aattagttta
     6121 ggacagatat acggatcggt gaaattcgcg gtccctatag ctacagcact tgctccagcc
     6181 ataaacatct caagaacatc atccactgtc tgcacgccgc ccatcccaat gattggaagg
     6241 ctggatatac ttgctacttg gtgaatcaaa cgaatagcga caggcttgat tgctggtccg
     6301 gagagtccac ctgtttgatt ggctaaaatc ggttttcttg tcttaaggtc aatccgcata
     6361 ccaagaagag tattgatcat cgtaaaccca tctgcccctc cagcttcgat tgcctgagcg
     6421 attggaacga tgtctgttac atttggggac aatttgacat agacaggaac agaagaaact
     6481 ttctttactg cttgtgttaa ttgaaaagca acatcagaat ctgttccaaa agcaattccg
     6541 ccatgtttga catttgggca agaaatgttc agctcgattg ctttcacatt tggtgcttcg
     6601 ccaattttgc tacaaacttc tacatagtct tcttcacaag caccagcaac atttgcgatg
     6661 atgggtaact cattgtactc ctctaattct ggaagaagtt tatgcataac aacgtctaga
     6721 ccaggatttt gcaaaccaat tgcattcaac atgccagatg gtgtttccgc cactcttggc
     6781 gtgggattgc caaagcgggc atttggagtc gttgccttga ccatgatcga tccaagctga
     6841 ttcaaatcat agtattttcc gtattctttg ccaaaaccga aacagccgct tgccggcatg
     6901 ataggatttt ttaattctag gccaggaagt ttgaccgcta aaggattctc attcatataa
     6961 ttacctctcc tattttaaaa atcggtcctt cgtcacaaac tttcacactt tttgttcctg
     7021 tttcatcttc cggcgtatga caaacgcaag catagcaagc ccctatccca catgccatgc
     7081 gggattctaa tgataactgg acattttgat gcgaagaaaa cagctgttcc actgtcttca
     7141 ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc cgtcttcgtt cctaacaata
     7201 aattaccgac atttccgtgc ataccgtatg ttccatcgtc agtagcgatt cttgtttctc
     7261 ctagacgatt aaattctttt tcataataaa cgacttcatg tgtggcaaaa cctaaaaaat
     7321 gcgttggctt aattcctttt tcgactaact gttttgataa ctcatataga ggagggacac
     7381 cgattcctcc accgattata aatacttcgt ctcctgtatg aagattggac aaatcaaaac
     7441 cattgcctaa aggacccatg acatctaaat ggtctccggc agaaagagta gagaagactc
     7501 tagtcccctc accttccacc cgataaatga tacggcaagt atttgtttcg tggtcgatat
     7561 gattcaagct aatcggtcgg cgtaatagca agtctgcacg cggcacgaga atatgcaaga
     7621 actgtcctgg ttgctgcatt tcttttacta gatcaccttt caatgtcatt tcaaaaatct
     7681 ttggtgccag ctgtttttgc tgaacgattt ccatcatttc ttgtttcatc tgtcccctcc
     7741 tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc cgttaaatag ggttagttga
     7801 aaaagcacgg gattccatca ctcgaacgat cgcatctgct gtgtctaaag aagtaaacaa
     7861 tggaatacca tgttcaactg cttctcttcg gatgatgaaa ccgtctttag aagcatttcc
     7921 ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta ccatctctta ttaagtcgac
     7981 tacatttttt tctgctgctt cctcagaaat tttcgctact gtttcaacga ccagaccatt
     8041 tttttcgaaa taagcagctg tatgtttcgt tcctatcaga ctatagccaa tttctgtaaa
     8101 acgtttagct aattctagtg cttcttcttt tgtttcgtcc gcaatcgtaa ataatacggc
     8161 tccatattca ggcaagtgta atccagaagc ttcgaatgcc ttgtacaaag ctttgtctaa
     8221 ttgtctgtca gatcccatta cttctccagt cgatttcatt tcaggcccta agtaagtatc
     8281 aactttctgc aatttcgtaa aagaaaagac aggtgcttta acatgaactt cttttgtttc
     8341 tggataaagt ccgttttcat aaccaagtgt ctttagtttc tctcccaaaa tagctttcgt
     8401 cgcaatttgt gccattggaa ttcctgtcac cttacttaag aatggaacag tccgacttgc
     8461 tcgaggattg acttcgatca cataaacctc attttcatgg atgacaaact ggatattcat
     8521 catcccgaca caattcaaac ctaatgctaa gcggatcgtg taatcttcga tcgttttctt
     8581 gatcgattca gataatgttt gaggtggata cacagccatt gaatcccctg aatggacacc
     8641 agcacgctca atatgctcca tgatccctgg gatgagtact gtttcaccgt cacaaatcgc
     8701 atccacttcg cattcaccac caagcaagta acggtccact agtactggat gttctggaga
     8761 tgctttcaca gcatgttcca tgtaatcttc taaatctcgc tggttttcaa cgatttccat
     8821 cgcacgtccg cctaatacat agcttggacg aacaagaact ggataaccaa ttctcgaagc
     8881 gataaccacg gcttcttctt tacttgtagc agtatcccct aaaggttgag gaatatccaa
     8941 ctctttcagc gcttgctcga ataaatcacg attttccgca cgatccaagt cttcgatcgt
     9001 tgtccctaag atttttacac cagcagcagt caaaggttcg gctaagttga ttgctgtctg
     9061 accaccaaat tgaacgatta ctcctatcgg ctgttctagc tcgatcacat tcatcacatc
     9121 ttcgaaggtc aatggctcaa aatataattt atccgaaata gaaaagtcag tcgaaactgt
     9181 ttcaggattg ctgttcatga tgattgcttc gtatcctgcc tgctggattg ctttgacaga
     9241 atgcacagtc gcatagtcaa attcaacccc ttgtccgata cggataggac cagatcccag
     9301 tactagaatt gagggctttt ctgatcgtga actttcattt tcatattcat aggaactata
     9361 aaaataaggt gtttttgatt cgaattccgc tgcacaagtg tcaaccatct tatatacagg
     9421 aatcaaatca ttttcttttc tcatctggcg aacggcttct tcgtttattt cccacagctc
     9481 cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt ttcaatatct tgtcatccaa
     9541 cggctgctgt ttcaactgct gttcgatttc tacgatatgc aacagtttat ctaaaaagaa
     9601 taaatcgatt tttgtcaaat ccgctaattc ttctatggtg taccctcttc taatagcttc
     9661 agctacataa aacaggcgat catcttgtgc tcgaaccact ttttggatta ggtcatcgtc
     9721 cgtgatgtct gccaactcag gaagttcgat atggtgtgta ccgatttcta aagaacgcac
     9781 agctttcaat aaagactctt cgatattccg accgatagcc atcacttcac cagttgcttt
     9841 catctgcgtg cctaaaagcc gttctccgtt ttcaaactta tcaaacggcc agcgaggaat
     9901 tttagctaca acataatcta atgcaggttc aaattctgca aaagtcgttc ctgtaacagg
     9961 atttttcatt tcgtctaatg ttaatccaag cgctatttta gcagctagct tagcgatcgg
    10021 atagccagtc gccttacttg ctaacgcaga tgaacgagaa acacgcggat tgacttcaat
    10081 cacataataa ttaaaactat gtggatcaag agcgagttgg acattacagc ctccctcgat
    10141 cttaagtgct cgaataatcg aaagagaagc atcacgaagc atttgatatt catggtcaga
    10201 caatgtttga ctaggggcaa atacgataga atcacctgta tggataccca ccgggtcaaa
    10261 attttccata ttgcagacaa cgattgcatt atcagccgaa tctctcatca cttcatattc
    10321 aatttcttta aaacctgcaa tacttcgttc aatcaaacat tgggtagcgg gggaaagact
    10381 taatccattt tctgatacga tacgtaattc ttcttcattt tcacacatac ctccaccagt
    10441 tccgcctaaa gtaaaggctg gacgaacgat gacaggatag ccgattttat ttgcaaaatc
    10501 tacagcttct tctactgtat tgacgatttc agactcgggg attggctgat tcaattcttt
    10561 catcaactgt ttgaatagat cacgatcttc tgcttgatcg atcgcagaaa gctttgttcc
    10621 tagaagttct atgttcaatt cttctaaaat acctgatgct gccagctcca ttgccatatt
    10681 gaggcctgtc tgtccgccta aagttggaag gagagcatct ggacgctctt ttcgaagaat
    10741 ccttgataca aattcaagtg tgatcggttc gatatatact tgatctgcca cttctttatc
    10801 cgtcatgatc gtcgcaggat tagaatttac taaaaccact tcatatcctt cttcttttaa
    10861 ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg gcttgaccaa tgatgatcgg
    10921 accagatcca atcaccataa ttttcttgat atctgtacgt ttcggcatct gtttactgct
    10981 cctttcctgc gtccatcatt tccataaatt catcaaataa atgtagtgca tcatgcggac
    11041 caggggcagc atctggatga aactgcacac tgaaagcggg ataatctctg tgtttgactc
    11101 cttctactgt tccgtcattt acttctacat gagttacgat gagtttttct ggatcaatcg
    11161 tggcttcatc gactgcatag ccatgatttt gagaagtaaa atcaattctt ccggtcgcta
    11221 cttctcgtac cgggtgattt aatcctcgat gtccgaattt cattttatag gtatctgcac
    11281 cgttggctaa agcaaataac tggtgaccga gacaaatacc aaaaatcggt acttttcctt
    11341 ggattttttt gatcatttcg attgcttctg gcacatcttt tggatcacca ggtccattcg
    11401 tcagcatgac tccgtctggt gatagcgcta aaatcgtctc ggcatctgtg ttatagggaa
    11461 gtactgtgag gttacagttt cttttactca attctcgtaa aatactatgt ttcaagccga
    11521 agtcaatgac aatgactttc cgacctgttc ctggacttgg ataaggtttg ttagttgaga
    11581 cttgctgcac ttgattggtt gctagaacgg ttgctttcag ctgatcaaat gcatgttcga
    11641 aggaatctcc agcatcaact atactagctt tcatcgttcc gacttcacgg attttgcgag
    11701 taagggcgcg cgtatcgata cctgatattc caggaattcc tttttgtttc aaaaatgaat
    11761 ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc atgttctttt actactactc
    11821 ctttgcatgt tggagaaatc gattcataat catctctatt taccccataa ttacccacca
    11881 taggataggt aaatgtaatg atttgaccgt taaaactttg atcagtgata gtttcttgat
    11941 aaccagtcat acttgttgta aaaacgattt ctccgaagac actagcatct gcaccaaaag
    12001 cctttccttc gaaaactgtt ccatcttcta atataagcaa acgttccata cttttattct
    12061 cccttctgcc acacaagttt tccgttgaca aaagtcattt gtgtttcgcc ttttactttc
    12121 cagccgataa aaggtgtatt ctcccctttt gataaaaagt cttctttatc gatcgtgcaa
    12181 gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac caaccgttag cgttcctgca
    12241 ttcaaaccaa aaatctcagc cggtttaact gccaaccaat tgatcacttg ttccaacgtg
    12301 aatcgaccag tttctacaaa atgagtatag atcagttgaa acgctgtttc gcttcctacg
    12361 ataccaaatg gtgatttcat gaaactttgg cttttttctt ctaatccatg gggggcatgg
    12421 tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt cgatcaaagc ttctctgtcc
    12481 tcccgtcctc ttagcggcgg gttcattttc caaaagccga aatcttcggg aatatcttcg
    12541 tcgatcaaaa taagatgatg gggtgaaact tctgctgtta cgtggattcc tgcttttttt
    12601 gcgtctctga ttacacggac actttcttta gtagaaacat ggcaaacatg gtaatgaaca
    12661 ccagttgctt cagctagcaa cagatctcta gcaatttgag aagactcggt tacgcttaag
    12721 atcccgggaa gaccaagttc ttctgccttc ttgccagcgt gcataacgcc gccaaataat
    12781 aaagattcat cttctgtatg ggctactagg gctttattgt tttttgcagc ttctttcata
    12841 gccagataca tcgtcccagc tgtttggaca cctacaccgt catttgtgaa agcaaaagca
    12901 ccttcttcaa tcagtgcttc ttgatcaact aatttctcac ttcgcagatt ttctgtgatt
    12961 ggcgcatact gcaatacctt cacaactgca tcttttctaa tgatgtcata tacttgtctc
    13021 agcttttctg ccgtatcagg tacaggattt aaattcggca tcgcgcatac agtagtgaaa
    13081 ccgccacgag cagctgagcg agtcccagct tcgatcgtct ccttgtatgt aaatcctggc
    13141 tctctcaaat gaacatggac gtccactaaa ccaggtgtga tcaattgtcc ctttgcatca
    13201 aacacttcat caaattcggc ttctgagaat cctgtaccga ttgctttgat tcgcccatct
    13261 tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg tattgatctg accgttttta
    13321 atgagtgttt tcattttttc atcctcctat aattacgctt ttccatttag cactgcttct
    13381 aagattgcca ttctgacgaa tactccattg ctcatttgct gaacgatccg cgactgtaat
    13441 ccttccacta atgaatcagc taattctaca tcacggttaa ctggtgctgg gtgcataatg
    13501 attgcatgtt tttgcaactt cttcgcacgc tcaactgtta acccatgttc ctgatggtag
    13561 ccttcttttg agaaactttc ttttccatca tggcgttcgt gttgcacccg aagcatcatc
    13621 atgacatcaa cttgatctag tagttcatcc aatggcatat aatgtccgta cacttcgaat
    13681 tcatcgtcgt accattctct aggacctgag aagaatactt gtgcgcctag acgttttaac
    13741 atctgcatat tggatttagc tacgcgagag tgagtgatat cacctacgat ggcaactttc
    13801 agaccttcaa aatgaccaaa ttcttcataa atggtcatca aatcaagcaa gcattgggtt
    13861 ggatgctgtc cgcttccgtc tcctccattg ataatcgaac actggattgt tttactttgt
    13921 atcagttcgt cataatagtt ctcatcacca tgacggataa ccgctacgtc tacgcctaat
    13981 gctgacatcg tcagtactgt atcgtatagt gtttcccctt tttggacaga gcttgtgctt
    14041 gcttcaaatt caatcacttc tagccctagt tttttctccg ctacatcgaa gcttttatgt
    14101 gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt actggcgctt ttgcggagtc
    14161 caattagcac cttgcttgaa ttcctgccct ctgcggatca agcccataac ttcttgatca
    14221 gtaagtgcct caacggtcaa taaatgcttt aagctgatac gttctgattt gatgatcata
    14281 atgtttcctc cttagttttc ttcgcttcgt gaagtttctg gtaagataag atttaaaatg
    14341 atgccaagta cagttgctaa tgccattgcc gacaatgtaa atgtgcctac ttcaaagacg
    14401 agtccgccaa ttcctactac taagataact gaagcaatca acagattttt ctttttatca
    14461 aaatcgattt tgttctcgat taaaatcttc attccacttg cggcaattac tccaaataag
    14521 ataaaactga ttcctgagat gactggtcca gggatactta aaatcaaagc gcttaatttt
    14581 cctacgaatc ctaaaatgac tgcaaatact gcagctccac cgatcacaaa cacgctatgg
    14641 acacgtgtga tagccagaac accaatgttt tcaccgtaac ttgttacagg aggacctccg
    14701 acaaaggctg ccacgatttg tgctaatcca tcacctgtca acgtcttatg aagacccggt
    14761 tcttcaaaga aattacgttt agttagttta ttcaatacca taagatggcc gatatgctct
    14821 gtcatcgtca caaaagcgat gggtgccatt gttgtgattg ctccaagata tagctttggt
    14881 tcatattgta caaagggaat ctcaaagtta ggaacagcaa accaggcagc gtctacgatt
    14941 ggcttagtat ccacgattcc aaacagcaag gctacgatgt atccgctaat aatacctagt
    15001 aaaatcggaa tcaatccgag aaaacctctt agccacatgt tgaagaaaat tgttaatcct
    15061 aatgtgatca gcgctacagc aatatacttg aaatcatacg tgccctgatt atacatagca
    15121 tttgttgctg catttgccgc aagtcccaac ccgataacca tgatgactgg accgacgacg
    15181 attggaggca agattttatc cagccatgct gaaccgatct ttttgatgat cagcgcaaca
    15241 atcaaataga ctaatccagt tgtgactgct ccttgtgcaa tcgctggata accatctgtt
    15301 ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc ttcctaagta cgcaggtatc
    15361 tttcctttag tagtaaataa gtacaccaat gtacctagtc ctgaactgac tagcgcaata
    15421 cctggatcga tacctactaa gatggggact aacacggttg ctccgaacat cgtgaacaag
    15481 tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc gatcgtggat atctaacacc
    15541 acgtcatcat tatgaaatcc ttttttctcc gacatgtttc tctctcctct aatcttcctc
    15601 tttcaaaatc atgatgcgat cttgtccatc aagctcttgc atctctacta aaatctcttc
    15661 cgcccgagat gttggaatgt tcttcccaac ataatctgca cggattggca attctcgatg
    15721 cccacgatcg accaggactg ctaatgaaat ttttcttggt ctgccaaaat ccattactgc
    15781 gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca tcatcaatca aaataacttc
    15841 ttttccttct aaggaaactg ggatgtcaga agaatgaagt tccggttctt ctggattttc
    15901 ttttttgtca tctcggtata aagtgatatc cagttctcct acaggaatat cgatatcttc
    15961 tagctgcttc aggcgttcgg caattcttga tgcaatgtag attcctcgtg ttttgatacc
    16021 taccaatacg atatcttgga tactatggtt tctctcgata atctcataag taattcgagt
    16081 caatgctcgc ttcatggtta cctgatctac tacttctttt gcttgcattt tcattccccc
    16141 tacttttttg cataaaaaaa ctcctgccct aagaggacag gaggaatgtt tagactgagc
    16201 gtatactgat acactgatag ctatactatg tcaatgagca atctacttat ggcgcaccat
    16261 acgtatcctt gctcccaccc ttcttagcct cacgggacta aacttaaagg aattctatta
    16321 agttttatgc tttcgttgtt aattactata ccgttatcta aattaaaatg caatgttttt
    16381 tcttaatttt tctaaagttt tttcaaaaac ttctggaaga ggtgcttcaa agatcatctt
    16441 ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca tggagaaact gaccattgcc
    16501 tttcaaagtt ttcttcggtc cgtatactgg atctccagct aacggataac cgatatactt
    16561 catatgcaca cggatctggt gtgttcttcc ggtttctagt tttaattcaa ccagagtaaa
    16621 tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc gacttcccat cttctcttat
    16681 cgcctgcatt ttccggtttt ccttcgaacg gccgattggc gcattgatgg ttcctttttc
    16741 gtgaggaatc tctccatgga ctaaagcaac atatttgcgc aaagaggtct tatcttttaa
    16801 ttgctgagct aatgattcgt gtgcctggtc atttttggca accattagca gtccggaagt
    16861 atctttgtcg attcggtgaa caatacctgg acgaataacg ccgttgatcg tagaaagatc
    16921 cttgatatgg tacatcagcg cattgaccat tgtgccatgc gtatgacctg ctgaaggatg
    16981 aacgaccatt ccttgaggtt tgtttatcac cgctacatct tcatcttcgt aaacgatcgt
    17041 caacgggata ttttctggga ccacatctaa tgtttctggt tcggggatca agacagtaat
    17101 ctcgtctcct tgttttactt tgtaatttgc tttgactggc tgtccattga cagaaaccgc
    17161 tccatttttc agccacagct gaatttgcga acgactgtaa tcggtgaata aattagtcaa
    17221 tactttatcg atcctaccag attcatggtg aatcgtcgtt ttaagttctg tcattttttt
    17281 ctttgatcct tttcatctaa aataatataa attaacatac aaatcactcc acatacaagt
    17341 gtcacatctg ctacgttaaa aatcggaaag ctcataaaat ctgtctggaa catatctact
    17401 acatagccaa gacgaatacg gtcgataaaa ttgccaatcg caccagctag aatcaaactc
    17461 aatccaatca tgaaccagcg attacctttt tcacgatttt taatcatcaa tgtgagaatc
    17521 acaacgactg caatgagtgt cactatagta aagaaccaca tcttcccttc tagcatactc
    17581 catgcggctc cagtgtttcg aatatgattc agtgacaaaa tacctggtat aagatttttc
    17641 gtctctccaa gcgctagatt atcagtgatc caccatttga ccaactgatc cactcctact
    17701 agcaaagcgc tgaatatcca ataaataatt aacaaaaaat caatcctttt ctatttgttt
    17761 ataaatatca cgaggtcgta cgatcccgag cagattacca tcgggattcc catctttagt
    17821 gataagtacg gcttccaaag tcccttgctc acgaaacatc gcttcaacct cgtaaatatg
    17881 tgttttagct ggaacaaaat gataatttgt gtactttccg ttttggatga tcaaatccaa
    17941 cgctcgttta tttgttaaat caaccggacc tttttggctt tcttttgcta gccaaaaccc
    18001 gatattacgt aaagtaatca atgctacaaa ttttccttta ttgtataacg gaaattgtga
    18061 ataccttttt tctgctacta tctttaacag ttcaagtaac ggaagatctt gttcaaaacc
    18121 tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc tgaatcagtt cttgttcgat
    18181 cagctggatt cgcttgactg tccaatcatt tggctctgca atgacaaaat cgactgcgat
    18241 ccgatcatgg acgatcgcat tccttaactg agcaagctgt aaaagatcat cttcgtattt
    18301 tctgatcatt aattgttttc tttgagctag gcgtctgaca agttcggtaa atcccatatt
    18361 tcgtgcattt cccatctcgt ctctcatcca tttttcgatt cgattgaaac tgattaaaaa
    18421 aagatcagag tttgccataa ttttcctcct tgatcaaata tgcccggttt tttatataga
    18481 aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa aaataaattt tgtaggaaaa
    18541 cggcccccaa cccagttgct agaaataaaa acggatagat ttgaagaatc aagtaattca
    18601 ttgtgatcac ataagcaggc tttgcttctt tcactgtata aacggggctt tttagccaat
    18661 aaaaggtgac ctgcatcgta aagggcactt ccagatcaag cgttatcttt tgttcatgac
    18721 tgagaacact gcgtttttca ttgttgattc ttagctgaat cgagctaccc ataccgtaaa
    18781 aaccggtttt tcttataaat gtcgttttca tcataccctc ctattatgct ttagtatagc
    18841 tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac aaaattaatt ccctatcgtc
    18901 taaaaaaaga agctgtagtc aaaatacagc tccttcgatg gtagattata atcaagctta
    18961 aaacaatccc aacacttctc cgtcatttgt cacgtccata ttcaatgcag caggtttttt
    19021 aggcaaacca ggcatcgtca taacatctcc agtcaaggct actaaaaatc ctgcccctaa
    19081 ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc ggtgcaccta gcagtgaagg
    19141 atcatcagaa aatgaatact gtgtttttgc catacaaatc ggcagtttgt cccatccatt
    19201 cttcgtaaac tcttctattt gtttttgcgc ttttttgctg aaaacaacac catttcctcc
    19261 gtaaattttc ttgacgattg tttctgtctt ttcttgtatc gtttcttcta ggcggtataa
    19321 tggcttgtaa ttagcttctt ttctatcaat caatcgtact acagcttttg ctaaatcaac
    19381 gccgccttct gctccatctg cccatacact cgctctcttg gcaagaatcc cctgattttc
    19441 acacaagtgc tccaacagtg tgagttcatt gtctgtatct gttacgaatt cattgattgc
    19501 gacgatcaca ggtagctgat attgttccat attacgtata tggcgcttta aatttgcaaa
    19561 acctactttc aaagcatcaa gattctcatt tttaagttca tcttttttca ttccgccatg
    19621 cattttcaat gcacggacag tggcgacaat caccacagca tccggcgctt ttttcaagtt
    19681 aggtactttg atatctaaaa atttctctcc gccaagatct gcaccaaatc ctgcctctgt
    19741 gacaacgtaa tctgctaatc tcaatgctgt cttggtagct aaaatactat tgcagccatg
    19801 agcgatattg gcaaacggtc ccccgtggac aaaagctggt gttccgtaaa ttgtttgtac
    19861 aagattcggc ttgattgcat ctttcagcag taatgccaga gcgccttctg ctttcaagtc
    19921 tccagcagtc acaggctgac ggtcaaatgt atagccgaca acgatccttg caagacgagc
    19981 ctttaaatct tcaaggtctg ccgcaagaca caagatagcc atgatttcgc tggcaactgt
    20041 gatatcaaac ccatcttctc ttggaacacc ttggattggc ccacccaagc caacaattac
    20101 tttacgcagt tcacgatcat tgagatcgac tacccgtttc caaatcactc ggcgggcatc
    20161 gatgttcaat tcatttcctt gatgaatatg attatctaaa agagcagata atgcattatt
    20221 agctgttgtg atagcatgca tgtcaccagt aaaatgcaag ttgatatctt ccataggcaa
    20281 tacctgtgca tatcctccgc ctgcagcacc acctttgatt cccatcacag gaccaagtga
    20341 gggttctcgc aatgcaataa cagatttttt attgatttta ttgagcgcgt ctcctaaccc
    20401 tacagtgatc gttgactttc cttcgccagc tggtgttgga ttgatggaag taactaagat
    20461 caaatgcccc tcagcgttat cctttgatcg attgattcca gaaaaattga ttttcgcctt
    20521 atatttccca tacagttcca aatcgtcttc cttaagatct aatttactag ccacttcaac
    20581 aatcggttct agctctgttt cttgtgaaat ttgcagatca ttcttcattt gattacccct
    20641 tttcacgaac tttattcaac aaaatatctt ctttgttaag atatgccaat tatacctttt
    20701 tttattcaga aaaaaaacat aaaattacag gtttttaatt tatttgacgt acttataaat
    20761 actctttata atgaaggcta tgatcagact ttagaaaggt cgtgagtgct ttgaccacag
    20821 gaattgtaaa atggtttgac aacaaaaaag gatacggctt tatcagttat gatgacacag
    20881 aagaaatttt tgtacatttt actgcgattg aagaagaggg attcaaaact ttggaagaaa
    20941 atcaagtggt cgaatttgag atcatcgaag ggaatcgagg aacacaagct gcccatgtca
    21001 agaaaggcaa cgttcagact accgatacta gtatgcatga atagataaac aaatagagtg
    21061 agacgctaaa cgggatgaac cgtacgtctc actcttttct tttcctgtaa aaattcgttt
    21121 ttttaggttc tgatttttaa aattagcttt ttcttttttt cgtataagga taaggaattg
    21181 attctgattt gggtccgttt ttttcaaaat gatcaaaaag gactacttga tctttttttc
    21241 gcatcatgaa aacagatggg gctttctcat cttgcaacat catttgcacc ccagtattag
    21301 ttggcgtcaa agatcgaatc tgtccgacag atatttctcg gtctttccaa aaagcagcat
    21361 aacgtataat cacagagttt gattttaaaa taaaagaacg ccgcaaacct agaaatagaa
    21421 aaacaagaaa aatacctaaa acaaaattgc ttttccaata aggacctgtg ttttctaaag
    21481 aaagaatcag actaaaaaac agaatgatca aggtcaatga ccaatagatg atcgtatgtg
    21541 ccagttcagg ctgccatcga atattttttt tcattctcta acctctcacc gattcagtaa
    21601 tgatataatc ttaccataac attagtttga acaaaaagga gaatctcttt ttatgttaaa
    21661 agtatacatt gatgcctcta caaaaggcaa ccctggtcct agtggaggtg gaatcttgat
    21721 tgtctatcaa ggtaaacaag aacagctatc tatcccgctt tctataggga caaaccatca
    21781 agcagagttc gaagtctttt taaaaacttt agaatttcta aagaaaaacg acttacagaa
    21841 tgaaacgatt ttctgttttt cagacagcaa aacactagtt tctacggtcg acaaaaacgc
    21901 aacgaagaac gagctcttcc ttccctacct tcaaagaatc caagaacttc tatccgaatt
    21961 ttctttattg atcctgcaat ggttgcccga aaacaaaaat aaaggagcag ataatcttgc
    22021 acgtcaggct ctccataaac aattaaaaga aaaaaagact ct
//
LOCUS       DAZULY010000017        21988 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000017 DAZULY010000000
VERSION     DAZULY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.017"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACG81A_000465"
     CDS             <1..395
                     /locus_tag="ACG81A_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS5013859.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="ACG81A_000466"
     CDS             398..1147
                     /locus_tag="ACG81A_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5013860.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="ACG81A_000467"
     CDS             1159..1428
                     /locus_tag="ACG81A_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013861.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="ACG81A_000468"
     CDS             1433..1597
                     /locus_tag="ACG81A_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013862.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="ACG81A_000469"
     CDS             1590..1892
                     /locus_tag="ACG81A_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013863.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="ACG81A_000470"
     CDS             1889..2050
                     /locus_tag="ACG81A_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS5013864.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="ACG81A_000471"
     CDS             2047..2352
                     /locus_tag="ACG81A_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013865.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="ACG81A_000472"
     CDS             2352..2708
                     /locus_tag="ACG81A_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFS5013866.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2668..2913
                     /locus_tag="ACG81A_000473"
     CDS             2668..2913
                     /locus_tag="ACG81A_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013867.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="ACG81A_000474"
     CDS             2910..3329
                     /locus_tag="ACG81A_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFS5013868.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="ACG81A_000475"
     CDS             3326..3883
                     /locus_tag="ACG81A_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFS5013869.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="ACG81A_000476"
     CDS             3880..4176
                     /locus_tag="ACG81A_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013870.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="ACG81A_000477"
     CDS             4253..4666
                     /locus_tag="ACG81A_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS5013871.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="ACG81A_000478"
     CDS             5012..5386
                     /locus_tag="ACG81A_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013872.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="ACG81A_000479"
     CDS             5423..5590
                     /locus_tag="ACG81A_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013873.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="ACG81A_000480"
     CDS             5616..5960
                     /locus_tag="ACG81A_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFS5013874.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="ACG81A_000481"
     CDS             5965..6246
                     /locus_tag="ACG81A_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013875.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="ACG81A_000482"
     CDS             6349..6663
                     /locus_tag="ACG81A_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFS5013876.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="ACG81A_000483"
     CDS             6641..8335
                     /locus_tag="ACG81A_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS5013877.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="ACG81A_000484"
     CDS             8355..9533
                     /locus_tag="ACG81A_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS5013878.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="ACG81A_000485"
     CDS             9496..10182
                     /locus_tag="ACG81A_000485"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFS5013879.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="ACG81A_000486"
     CDS             10182..11342
                     /locus_tag="ACG81A_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS5013880.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="ACG81A_000487"
     CDS             11352..12227
                     /locus_tag="ACG81A_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013881.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="ACG81A_000488"
     CDS             12224..12535
                     /locus_tag="ACG81A_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013882.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="ACG81A_000489"
     CDS             12525..12878
                     /locus_tag="ACG81A_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS5013883.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="ACG81A_000490"
     CDS             12868..13269
                     /locus_tag="ACG81A_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013884.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="ACG81A_000491"
     CDS             13262..13666
                     /locus_tag="ACG81A_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013885.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="ACG81A_000492"
     CDS             13678..14286
                     /locus_tag="ACG81A_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS5013886.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="ACG81A_000493"
     CDS             14306..14668
                     /locus_tag="ACG81A_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013887.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="ACG81A_000494"
     CDS             14671..14853
                     /locus_tag="ACG81A_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013888.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="ACG81A_000495"
     CDS             14870..18289
                     /locus_tag="ACG81A_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS5013889.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="ACG81A_000496"
     CDS             18340..19077
                     /locus_tag="ACG81A_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS5013890.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="ACG81A_000497"
     CDS             19087..21378
                     /locus_tag="ACG81A_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFS5013891.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="ACG81A_000498"
     CDS             21402..>21988
                     /locus_tag="ACG81A_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFS5013892.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3245 c         4705 g         6105 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatttg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DAZULY010000018        21901 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000018 DAZULY010000000
VERSION     DAZULY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.018"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACG81A_000499"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACG81A_000499"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFS5013893.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACG81A_000500"
     CDS             complement(1172..1867)
                     /locus_tag="ACG81A_000500"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFS5013894.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACG81A_000501"
     CDS             complement(1880..2596)
                     /locus_tag="ACG81A_000501"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFS5013895.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ACG81A_000502"
     CDS             2926..3534
                     /locus_tag="ACG81A_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFS5013896.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="ACG81A_000503"
     CDS             3859..4182
                     /locus_tag="ACG81A_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFS5013897.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="ACG81A_000504"
     CDS             4364..5734
                     /locus_tag="ACG81A_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFS5013898.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="ACG81A_000505"
     CDS             complement(5864..7165)
                     /locus_tag="ACG81A_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS5013899.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="ACG81A_000506"
     CDS             complement(7519..9510)
                     /locus_tag="ACG81A_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFS5013900.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="ACG81A_000507"
     CDS             complement(9654..9920)
                     /locus_tag="ACG81A_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5013901.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACG81A_000508"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACG81A_000508"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFS5013902.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACG81A_000509"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACG81A_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFS5013903.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /gene="def"
                     /locus_tag="ACG81A_000510"
     CDS             13264..13827
                     /gene="def"
                     /locus_tag="ACG81A_000510"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS5013904.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="ACG81A_000511"
     CDS             complement(14080..14601)
                     /locus_tag="ACG81A_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFS5013905.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="ACG81A_000512"
     CDS             complement(14604..15446)
                     /locus_tag="ACG81A_000512"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFS5013906.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="ACG81A_000513"
     CDS             complement(15439..16164)
                     /locus_tag="ACG81A_000513"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFS5013907.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="ACG81A_000514"
     CDS             16340..16636
                     /locus_tag="ACG81A_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013908.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16892..17066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17204..18796
                     /locus_tag="ACG81A_000515"
     CDS             17204..18796
                     /locus_tag="ACG81A_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5013909.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="ACG81A_000516"
     CDS             18809..19549
                     /locus_tag="ACG81A_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5013910.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="ACG81A_000517"
     CDS             19717..21066
                     /locus_tag="ACG81A_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5013911.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="ACG81A_000518"
     CDS             complement(21194..21784)
                     /locus_tag="ACG81A_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS5013912.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6289 a         4461 c         4158 g         6993 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 a
//
LOCUS       DAZULY010000019        19916 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000019 DAZULY010000000
VERSION     DAZULY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.019"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..287
                     /locus_tag="ACG81A_000519"
     CDS             3..287
                     /locus_tag="ACG81A_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013913.1"
                     /translation="MKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLASKKT
                     NSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(931..1884)
                     /locus_tag="ACG81A_000520"
     CDS             complement(931..1884)
                     /locus_tag="ACG81A_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5013914.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1907..2662)
                     /locus_tag="ACG81A_000521"
     CDS             complement(1907..2662)
                     /locus_tag="ACG81A_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5013915.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2659..3618)
                     /locus_tag="ACG81A_000522"
     CDS             complement(2659..3618)
                     /locus_tag="ACG81A_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS5013916.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3611..4561)
                     /locus_tag="ACG81A_000523"
     CDS             complement(3611..4561)
                     /locus_tag="ACG81A_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS5013917.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4711..5163
                     /locus_tag="ACG81A_000524"
     CDS             4711..5163
                     /locus_tag="ACG81A_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFS5013918.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5252..7519)
                     /gene="gshAB"
                     /locus_tag="ACG81A_000525"
     CDS             complement(5252..7519)
                     /gene="gshAB"
                     /locus_tag="ACG81A_000525"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFS5013919.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7706..8704)
                     /gene="ftsY"
                     /locus_tag="ACG81A_000526"
     CDS             complement(7706..8704)
                     /gene="ftsY"
                     /locus_tag="ACG81A_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFS5013920.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8715..9524)
                     /locus_tag="ACG81A_000527"
     CDS             complement(8715..9524)
                     /locus_tag="ACG81A_000527"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5013921.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9533..13114)
                     /gene="smc"
                     /locus_tag="ACG81A_000528"
     CDS             complement(9533..13114)
                     /gene="smc"
                     /locus_tag="ACG81A_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFS5013922.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13134..13820)
                     /gene="rnc"
                     /locus_tag="ACG81A_000529"
     CDS             complement(13134..13820)
                     /gene="rnc"
                     /locus_tag="ACG81A_000529"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFS5013923.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14168..15949)
                     /locus_tag="ACG81A_000530"
     CDS             complement(14168..15949)
                     /locus_tag="ACG81A_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5013924.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15975..16889)
                     /locus_tag="ACG81A_000531"
     CDS             complement(15975..16889)
                     /locus_tag="ACG81A_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5013925.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16916..17878)
                     /gene="opp4B"
                     /locus_tag="ACG81A_000532"
     CDS             complement(16916..17878)
                     /gene="opp4B"
                     /locus_tag="ACG81A_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS5013926.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17878..18822)
                     /locus_tag="ACG81A_000533"
     CDS             complement(17878..18822)
                     /locus_tag="ACG81A_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5013927.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18823..19827)
                     /locus_tag="ACG81A_000534"
     CDS             complement(18823..19827)
                     /locus_tag="ACG81A_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5013928.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5536 a         4273 c         3539 g         6568 t
ORIGIN      
        1 atataaaaaa catctatttt atcgaagaaa cacaaaatat cgagggttct tatattgaag
       61 taaaaacact ctttgtcaat gaagataaaa ccaaagcatt ggatatttat aaaaaattag
      121 cttcaaaaaa aacgaatagt tttggattga tattaagcga gtataaaatc aaagcagaag
      181 agagttattt ctatcaatta ttaaaacggt ggagtaagct tcccgccgat ttttatcgaa
      241 aaatgcagat catcaactat caaccgcttg ctgagacaca tgcctaaata gaggttgtga
      301 aatcagtgct ttgagctaat accgaaggtc agctgattga acattgttta gaagagattg
      361 tgacaactgc gatttgcttt gagtaataag aacgctggac agaaaatagc ttctcatatt
      421 ttctagtcta gtgggcttat tgccgagaag cagcagatgg aacatcgttt agaagagatt
      481 gtgacaactg cgatttgctt tgagtaataa gaacgctgga cagaaaatag cttctcatat
      541 tttctagtct agtggactta ttgccgagaa gcagcagatg gaacatcgtt taaagaagag
      601 atcggtacaa aataaaaata tctaaattta agttttatct aagtttcgct ttgtaccttt
      661 gattcaaatc aaaggaggcg atcttgatgc tgaaaaaatg gatcacacta ttttttgcca
      721 ctccccattc tagaatcaac ccgtatgatg tagataatga attcacgttg aaaaaagatt
      781 tctcaaataa ataaaaacca tccgtaaaat catcaatacg attttacgga tggtttttga
      841 taacttcttt gaggataggc tcttgctatt taagaatgta agaaacatta ttgttacata
      901 gtatgagagt ttaatcctct gttatttatg ctatttaaat gcttgattca catcttcgat
      961 catcagtttc attgattcta aaccgccccc gcttaaatac cagacatctg gttcaaggga
     1021 gatgatctgc tgatttttac cagcattcgt ttgtgctacc agttcattgg cagaaatatc
     1081 atctttgctg tcgtctccac caatagcttt tgttctgtct actacaaata agatatccgg
     1141 atttttttcc agcacatatt cataagaaac actttgaccg tgagtggatg cttcgatttg
     1201 atcatctgct tgttcaaagc caaataaatc atggatgaaa ccgaatctag aaccagatcc
     1261 gtaggctgat aattgccctt cattcacaag tgtgactagt gcttttttgt cagtagcttc
     1321 tgctttcttt ttcgtctgat cgattgcact agataattcc tctaattgag ttttagcttc
     1381 ttcttgctta ccaaagatct cccctaaagc agtgacattt tgctggaaag attcccatgg
     1441 atttttcgca tctaaagaaa ggaaaatggt cggagcaatt tgacttagat ctttttgaaa
     1501 atctctttgt cggccagaaa tgatgattag atctggctga agctgattaa ttttttctaa
     1561 gtctggttct tttattccac ctgctgattc tactttttta taggatgaaa gatactcagg
     1621 aaggctgctt gtagctgctc caactacctt atctccaaca ccaagagcat ccatcgtatc
     1681 taaagaacca ttgtcgaata cgacgacttt ttctggatta tttggcacct cgatttggtc
     1741 tccattacta tctgttatcg tcaatgtagt ttcagtttgt gtgctgcttt tttttgtgtc
     1801 tgcctgactg gtgttgttgc tatttccaca tgctgctaaa ccaaatgatg ctagaatcaa
     1861 gatacctact actaattttt tcatctcttc tctcctaatg ttagttttag aaataaagac
     1921 agaatctgcg gccttcaatc tcacaaatcc gtatgtccat ttcgtataat tcatccaata
     1981 catctttttt tatcatttct tgggtcgttc cttgcgtaaa taactgtcca tctttcattg
     2041 caacaatttc gtctgcataa cttgcagcaa agttgatatc atgaagaaca ataacaattg
     2101 ttttcccgat ttcttgtact aaatttttta aaatctgcat gatttgaacc gcaaaattca
     2161 tatctagatt gttcaaaggt tcatccagta aaatataatc cgtgtcttgc gctagcacca
     2221 ttgcaatata cgcacgttgg agttgaccgc ctgaaagagt gtcaatcgat tgatccgcta
     2281 tttcttctaa cccaaggtgt tccagtgatt cctgaacgat ttcctcgtct tttgtcgtca
     2341 attttccttt cgtataagga aatcgtccaa attctactaa ttctttcacg gtgatttgga
     2401 catttatttc attcgattgt cgtaggatcg ataatttttt ggctaattca ttggatttcc
     2461 aagcctttac ttcgttatgg tctaaataga tttcacctgt atcttttggt atcaaacgac
     2521 tgatcatcga caacaccgtt gactttccag caccatttgg accgataaaa gcagtcaatt
     2581 tcttttcagg aatcgttaac tgtacatctt taaccactga ttttccatgg tacagtttat
     2641 tgaccttttg taaatcaatc ataggatact cttcctttcg cgaatcaatt ttgtgacaaa
     2701 ataaattcct ccgacaaatt caacgacgac actcactgtt gtattccagc cgaaaatctg
     2761 ctctactgcg agctgtcctc ctgtcagaaa cagcatagct accagaaagc ttccgataaa
     2821 tagctgacca tgacgataag tagaaaagac acgataactg atatttgcga caataaaccc
     2881 taaaaaagta actggaccca caagagccgt tgatgtccct gtcaaaagac tgacacaaca
     2941 taaaatcaaa aattcaaaac gatctgtgtc tatcccgagg tttactgctt gttcatttcc
     3001 gagatggaaa acgtctagca catgtcgata tcgaaataac aggtaccagc agccgataat
     3061 gatcaaagaa gcaggcaaaa gatactgact ctggatattg ccaaagcttg caaataatct
     3121 gccttgaagc aaatcatatt cattcggatc catgatgact tgtaaaaagg tactgacact
     3181 accaaaaagc gtacctaaaa tcatccctga catcagtaat aaaaaaagat tcgctctcat
     3241 ttttttcaaa agaaagaaaa ataacaatag actcagcccg accatcagcc caacgttcaa
     3301 taagaaaaaa actgtacctg tctgctgaag catcgtcact cctccgataa agaagaaaag
     3361 gaccgtttgg acaaggacat ataacgactc aagtcccaat actgaaggag tcaaaaactg
     3421 gttatgcgta attgtctgaa agctgattgt ggcactgctg acagctagcg agacaaggat
     3481 aaatgcaaaa attttcttac tcctcaatgc acatgcaaaa tgccagtttc catatgtatg
     3541 gatcgtcaag tacattcctg taacaaataa aacaagaaca agcataccaa taaaaataga
     3601 tggctttttt ttaagcatat cgtcgtcccc ttatcagaag ataaaggaag atagccccac
     3661 cgatgattcc aacgatcaca ctgactggta tttcataagg aaaaatcaac acgcgagcga
     3721 caagatcgca aaatactaag aacaaactac ctgctaaagc agttacgcca attgttttgt
     3781 gcacttgatc tccatatatt ctagagacaa gattcggaat gatcactccg ataaaaggaa
     3841 tgcttcctac catgatcaac atgactgcac tagccaatga tactaagcca atgcctaaaa
     3901 actgcatctt ctggtaatct attcccagat ttactgcaaa cgattcgccc agaccaatga
     3961 tcgtaaatcg gtaagcaaat agccaaacca agagcaacaa tggaacaaca gcatacaata
     4021 actcataatt tcctcgtatc acagtcgcaa aattcccttg aagccatgaa gtcatatttt
     4081 gtaccaactg gaattgataa gcaaaaaaag tagcaactga accaatgata ttccctaaca
     4141 tgactccaat caacggaagc atcaattgat ttttctggag gaacaatcgc gtaagggata
     4201 aaaaaagcaa tgttcctgca aaagcaaagc aaaatgctgt caaggatcgg acaatcaagg
     4261 atgcaccagg taggaaaatc atagccacta aaatacctaa acgcgcacta tccatcgtgc
     4321 ccgcagtcgt tggtgaaaca aatttatttt gggtcaaatg ttgcatgatc aacccacaaa
     4381 tactactggt cgctcccaca aggatcaaac aaatcgttcg tggcaaacga gtcactagca
     4441 agatttgtaa gctttgccca tctaactgga gcagttctga caattgtaca ttcgatactc
     4501 cgatgaacaa tgaaatacta cttaaaataa ctaataatgc tactagtcgt ccgtagcgca
     4561 tccaatccac ctttctaatt gagaatgatt ttcattctta ttttaagtga atcgttgaac
     4621 aaaagcaacc gctttttcat atgttttagg tattattggg aatatgctat aatcgtaaga
     4681 aagacaaaga gaagaaggag ggtatttttt atggcacaag atgatgtaaa aagccatttg
     4741 gataagggac tttatggtac acctttagtg aaccctgaag aacagcataa atatttaggg
     4801 acattcaggg aacgctgcta tcttagtatg actgttgctg aaatgatcca accaaaaaac
     4861 aaagaaaact ttttaaaaga actcaaaaaa catccagatg cttccatact cttgaatggg
     4921 gctctacccc tttctgtcca aaaccaatat atccagctag caacgaaaaa caatagccgt
     4981 ttcactgtag tcaacgatta cgtcactgca aatcctgagg aattcggatt gctcttaaca
     5041 gcaaaagaag cagtaaatga acctgtgatt gatatcgaaa aaaaataccc agaagaaaac
     5101 tcttcgccag ctgaaaaaga aaaaccaaaa aagaaaaaaa gcttctggga tcgtttattt
     5161 taaaacgaaa aaacacataa atttttacat gcaaaaaaag cttttgatca gattgacatc
     5221 aaataggaca atctgtcaaa agctttttct tttaaaatac ttcaggatat aacaatttca
     5281 atacattcat cgtcaatctt ctgccttttc ctgcaaaagg ataaacatgc atatgcatgg
     5341 ctggattgaa atttgcttcg atgatcccat atgctttttt atcagttgta ggatcgattt
     5401 ctttatctgg tataatcaaa tcgataccac taatcttagc tcctagtgct tctaccgcag
     5461 aaacggcgat ttttttgtaa gcttcagaaa attcatcagt catatcgatc gaatcaccac
     5521 ctgtactgat atttgagttc tcccttagat aaacaatttg attggcttgg ggaactgatt
     5581 cgatcgtcaa cccttgttct ttcaacatca attgttctaa ttcacctaat tggatcaatt
     5641 ctaaaggtgc acgatgattc gttccacgca aagggtcact atttttttct tcaacaagtt
     5701 ctttcactgt tcggatactg tcaccaataa cattagcagg gactcgcaac atgatggctt
     5761 ggacttctcc gtcgatcaca aagaaacgat attccgttcc cggcataaat tcttctacta
     5821 aaaccgaact gtcttcacga aaagcaatgg ctaaacctgc ttgataatct tccagactag
     5881 ctccttcctt gaaaatcgtg atacctaacc cgtaattggt tgatttaggt ttgatcacaa
     5941 atgcctgctc ggcaaatctc ggataagctt ttaccgcttc ttccatggat gaaaattctg
     6001 cgcctcctgg tacccggaac ccagcttctt ttaagacttt tttcgttact gttttgtttt
     6061 ccatgataag tgggacgata tagctgtcct tacttgtcat gtttgcattt ttcacatatt
     6121 ctacatggtc ttggaattgc agacgcaaaa attgatccga ctcatctaat actttgactt
     6181 ggacaccttt ttggatggca tcgaacataa atatctgagt agaaagttcc atttcgcgaa
     6241 aaccggccaa ctgataggga cgttcatggc tttctttata atattgatgt cccaatgcca
     6301 cagcaacttc ttgctgactc gatagctgtg cttcttgata gagtttcgct gctaacgttt
     6361 gttcaggatg atccatttga gtcaatgctt cctctactaa ataaagagaa tccaaccaat
     6421 ccaattcaac cagcatttgc ttcattcctt caagaatttc tctaccttct tgatgaaatt
     6481 ctgtttgatc tgatggttgt tctagtgcta ctttgttatt tcgagtcgta ccttctgcta
     6541 cccattggtc acagtcttct ttttcttctg tccataacat gtacatcagg aataattgta
     6601 agaaccgaac ttgctctgga ttgataccca atcttgcata aggattcaaa tcgatattcc
     6661 gcaattcaat ataacgaaca cctgttgttg ctaaatctgc gactttcttt ccgcctcgga
     6721 aacgaagcgg tgtataaaat tctttctcct ccgaaagttt cccttcttcg atcattcgct
     6781 cgatatctgc taaatactgt ttcatagaag cataggaaac tttcacattt ggatgattcg
     6841 tatagcccag cgcactgttg cgaatactgc gaacaggttc ttgcggatgt gattcatcta
     6901 aaaagtagtt cttctcactc attggtgaag caccgaataa ataagtgatc atccagcgat
     6961 agcgcataaa gtttctcgct gttttcaaat aaagttctgt tttgaattcg gaaaaatctt
     7021 gaaattcttc ctgatggctg aacaatgttc ttagcaactc atcgccaaat tcaaaattga
     7081 aatgaatacc gcttaccatc tgttttcgct tcccgtattc ttttgctaaa taacgtcgat
     7141 acaagacatc ttcgaaattt tttaattttg caataatgat ctcttcgtct ttttccggta
     7201 aggcaggtgg catacttaat ggccagatca tctcttcttt cggtatagaa cgagcagtca
     7261 catcataaac agcagcgaga tactgaaata attcgggaat agaatcggta acaggggtga
     7321 tcatctctgt ttgtgtttcg ctaaaatctg tttggatata ggggtgatag gatcgatcgc
     7381 caaagattgc tggatgatcg gtttttgcta gatttcctgc aagatcaacc cgctgtcctt
     7441 cccgttctat accaaatcga gcttggtcga taaaagggcg cgcattgaca tgcaataata
     7501 attgcttaaa attcatcatt aagtgacctc caattcatat gaatctaatt ataatcaaaa
     7561 agtctaagaa aaagaacgaa attgtttgag aaccgcttac aaataagtat aaaaatgaag
     7621 gcatcaaaaa cacagctatg ttaaaaacaa tcattcaaac aaggagctgc aacttttgtc
     7681 acagctcctg ctttttagac atgggttatt cttcttttag taatcctttg aacaatccaa
     7741 cgacaaaatc atttggatca aacggctcca agtcatcgat tccttcacca agaccgacca
     7801 atttcactgg taaatgcaat tcattacgga tagcaagtac gataccgccc tttgctgtac
     7861 catctaattt tgtcaaaaca agaccggtca catctgttgt ttctttgaat tgttttgctt
     7921 gaaccatcgc gttttgacca gtggttgcat ctaatacaag aagtgtttct tggggtgctt
     7981 ctggtaattc ccgctggatt actcgtttta ttttttctaa ttcgttcatc aagttcactt
     8041 tattttgcag tcggcctgca gtatcaacta gtaaaacatc tgcctgctct gtctttgctc
     8101 tttccaaagc atcgaatacg acagctgctg gatcgcctcc tgcattgcca cgaacaactt
     8161 caacaccagc gcgttcgccc cagacaacta actggtcgat tgctccagcg cggaaggtat
     8221 ctgctgctgc catcaatact tttttacctt cgtttttgaa ctgatgggca agtttaccaa
     8281 tacttgttgt tttccctaca ccattaacac cgacaaacaa aataactgtt agtccgtctt
     8341 cttggatatt tagttcattt acttcattga ttccttccgc ttcatagaga tccacaagtt
     8401 tctcgatgat cgcattttgg acttctgccg attttttcgc atttctgagt ttcacttctt
     8461 gacgtaatgc ttcggtaatt cgtatcgctg tttcaaaacc tacatctgca ccgataagtg
     8521 tttcttctaa ttcttcaaaa aagtcttcat cgactgttcg aaaattggca aacaactcat
     8581 tcatccgttc accaaatgtt ttacgcgtct tttctaaacc cttgtcgtac ttctcctgta
     8641 cttcaacttc ttctgttttc tcttcgccag taaaagcacg cttgatttta tcaaaaaatc
     8701 ccatttgact ccccctactc taatacaaat ttttggatca ctttggctac gccattttct
     8761 tcattgctgg ctgtcacgaa atctgctgct tctttgacga aaggcacagc attctccatc
     8821 gctacgccta gtccagcata ttggatcatc ggtaaatcat tttcctcatc accgatcgcc
     8881 atgatttctt cttgtttcaa accaagatct tttgccaata aagaaatacc ataagctttt
     8941 gtaacacctt ttggcataaa ttccaaaagc atacttcttg ttttgatgat ctcatacatt
     9001 tcatggaatt ctgctggtat ttctttgatt ttaggatcta attcttcttg gtcataagcg
     9061 attactgcct tattaagcac aaattcatct gtaatatcgc ttatatccgc tggttggaat
     9121 tgcagcagct tattcaacac attgtacaag gattgcttcc ttggggcact aggcagctgt
     9181 aaaaccacat tgtcagacaa aacatctagt ggcagatcca attgctgagc cagtttgtat
     9241 aagcgttgga tatctgatac ttccatcact ttcttttcca tgatttcacc agtatcattt
     9301 ttttgtacca aaccgccgtt gaaagtgatg ctgtagtcac cttcatctac cagtcctaat
     9361 tcttgcagat aatgcgccat cgcagctaag gggcgtcctg tacatagaac gatcttgacc
     9421 ccttgctgtt ttgcttgtgc taatgcttgt ttattctcat ctgaaatcag cttttgttca
     9481 tttaataatg taccgtccaa atcgattgct actaatttga tcattttcgt tcctacactt
     9541 tcgtttcgtt gattttcgcc tcttcattga tatcttccat ccgtacagaa acgatttttg
     9601 atacaccgga ttcctgcatc gtcactccgt acagtacatc tgctgcgacc atcgttcctt
     9661 tgcggtgtgt gactacgata aattgtgtgt catcttggaa atcgctcaag taacgaccaa
     9721 aacgtttcac gttggcttca tctaacgctg cttctacctc atctaagata caaaacggaa
     9781 caggacagac acggataatg gaaaagagca acgcgatggc tgtaagtgct cgttctcctc
     9841 cagaaagcaa acttagactt tgcagttttt tgccaggagg ttgtacttca atctcaatac
     9901 ctgttttcag caaatcagtt ggttccgtca gtactaattc agctttaccg ccgccaaaca
     9961 tatttggaaa gacaattttg aattcttgac ggatagcttc aaatacttct ttgaatcttg
    10021 tccgcacttc atcatccatc tcatccatcg tttcaaacaa ttggtttttc gctgctaaca
    10081 gatcatctcg ctgtgttgct aagaatgtat gtctttcact gacttgttcg tattgttcga
    10141 ttgaatttaa gtttactggt cccagttttt caatctgttc ttttaaggat gaaacctttg
    10201 ttctactgtc ttcgatatcg gttgtttctt gatagtcttg ggaagctttc tcaaatgtca
    10261 gctgatattc tgactgcaga taggtcaaat gattatctaa aatcatttca gctcggtctt
    10321 tttggacttc taatttggat tgttccgaca gtcgtgcttt ttgctcacga ttacgctctg
    10381 ccaagactgc ttcaagctta tcgatctcct tttgccttct atctcgctgc tctttggctt
    10441 ttgccaactc ggcttttaat gtttctcgct gagcagataa ttcgttaatt tgtttttcca
    10501 aactttcttc tgtgacttca tgatcagaaa agtctgctgt caatgtcgcc aactgctttt
    10561 ctatcgcttc ttgacgttcg gttgcctctg ctttttggac tcgggcaccg cggagctgga
    10621 tctgcagatg attaaactgc tctttcaaga cagcaagatc tgcttgttct tgtgcttttt
    10681 gactttgtac ttgtgctctt ctggcttcca tctggtcgct ttcttgactt aacgatttga
    10741 tttcttcgtc gattttttgt cgttgcgatt caagatctgt ttgtttgtct ttcaattcgt
    10801 tcgtttgctt ttcgtaatct tcgataaact gctgcagttc acgtgtctca aaagttgaga
    10861 tttgcttttc tttttcaaaa cgttcaagtt catttatgat gttttgtagc tgatttgtcg
    10921 cttcttgttc ttcaaaacgt aattgttcgc ctcgtgtacg caaaacttct tgttcttcgg
    10981 ctaaacgagc tgtttcttgt tgcagctcct gaactttttt ttcttgtgct tggagttgtt
    11041 tatctgcttc ttcaaattca ctagtcaact gtttcaattc ttgattttgt acaaacaaac
    11101 tgccttggtt tcctcgttta ttcgctccac ctgtcatgga tcctccggca ttcatcacat
    11161 caccttctaa ggaaaccact cgatattgat agcggatcgt ttgcgcgatt gcattggcac
    11221 tagttaagtc tttggctaaa aggatcgttc ctagcaggtt ttgaaccaca gtttggattt
    11281 gatcgggata actgacttgt tcactggcaa tccctataaa cccttctaca gctgctgctt
    11341 gtgtcaatat atgagccggt aattgtcttg gtttgattgt cgtcagtgga aggaatgttg
    11401 ctcttcctcc tctttgctgt ttcaaatacg tgatggcttg ccgagcatct ttttcatttt
    11461 caacgatgac atgctgggct gccccaccta aagctgtttc gatagctaac gtgaaatctg
    11521 caggaacatc gatcagttca gccactgctc cgacaatacc cgaaagctgc tgtttatgct
    11581 gcaagaccaa tcgaacccct tggtaaaaac caaaatagtt ttcttggatt tcttgaaggc
    11641 ttttcttacg agcacgtagc tgctggactt gattcattaa ttgatacatt ttaggctgtt
    11701 cttttgctaa tgcttcttgg aactttttcc cgttttgttg gatcatttct aacttctctt
    11761 ttgtttcagt taaagatgat tttagctgcg cctgcttttg tgtcaattct tctttttgta
    11821 agatcaatcg gtctactgat gcttctactt ccgattgctt ggctaacgtc tgcttgcttt
    11881 ttgctgtttc ttgtatatat tgacgttcta gatacttcag ttcgtttcct acagctgctt
    11941 gttcttgcat caaatcaaca tattgatctc ttaattctgc caataattct ttcgttgatt
    12001 tttggtattt ttctaattct tgctgtgttt ttatgagatt tgcttcagct ttttggatct
    12061 ctgtttcttt ttctgctgct gctttcatca agctttcttg aagcttttca aaatgtttaa
    12121 cctttttttg tgcttctgct aatgaagttt gatattcttg cgagcttttt tgtgtatgct
    12181 ttgtccgctc ctgcaaaaca tctttttgtc cttctgtctg cttcagtttt tccgaaagat
    12241 ctaataatac ttgctgattt ttttcgatca gacgatcggc ttgcgcattt tctttacgct
    12301 gcttcgccaa aatggattct tgttcttgga tcgactcaga tagtttgcct aattctagat
    12361 tgaatttcgc aagctgtgct tgtttgttgt cccaatcttt ttttgctgtc ttgatttcag
    12421 caaccatcaa agagacatcg gtttgagtca gtgtttcttt tagccgtaaa aactcctttg
    12481 ctgcctcact ctgggcggcc aaaggagtta actgttcttc caattcgtgg atgatgtctt
    12541 ggacgcggct gaggttgtct tctgtttcaa aaagcttctg ttctgctttc tttttccgct
    12601 gcttgtattt caagacacca gctgcttctt caaagatccc acgacggtct tccggtttac
    12661 tactgaaaat cgcttctact tttccttgag aaatgattga gaacgattct tttcctaatc
    12721 cagaatctaa gaacaattct tggatatctt ttaatctgca gctttgttta ttgatgaaaa
    12781 actcgctttc cccagtccgt cgataacggc gggttacact gatctcattg tactccaatg
    12841 gcagataatg atcactattg tctaaaatca ctgtgacttc tgcaatattc agctgcttac
    12901 gggtatcgga accggcaaag atgatatccg gcatttttcc gcctcgcaga cttttagcag
    12961 attgttctcc taatacccag cggatagcct ctgtaatatt gctttttccg ctgccatttg
    13021 gtccaaccac tgctgtgacg ctattttcaa aatcaatgac tgtcttgtct gcaaatgatt
    13081 taaaaccggc tatttcaatt cgtttcaaat acaaagcttt ccctcactta cttttacagt
    13141 gctgccaatg cattttccgc cgcagcttgt tctgctaatt ttttcgattt cccttgtcca
    13201 gtgccaatca attgatcatc tacataaact tcgatccaaa atactcgttc atgagctgga
    13261 ccttcttcat tgatcaatcg gtattcgatt gatacatcac ctgatttttg caagacttct
    13321 tgtagttttg ttttatgatc catctcatgt gaaaaagcac ctgctttgac tttaggaaaa
    13381 atcacttttt cgatgaatgc atgggtcgtg tcgaatccct ggtcaagata taacgcgcct
    13441 aaaaaagctt cgaataggtc gcaaagcaat gctggtcgcg tacgtcctcc tgaattctct
    13501 tctcccttgc ccaacaaaac atattgatca aagtgacatt ctttggcaaa tttcgacaag
    13561 ctgtcttcgc gaacgatcgc agctcgtgtt tttgtcaatt ttccttctgg caaatgagga
    13621 tataaacgga ataaatactg agaaaccatc agttctaaaa cggcatctcc taaaaattca
    13681 agtcgctcat tatccgataa ttgcagattg cgatgctcat tcacatagga tgaatgggta
    13741 aaagcttgtt caagcaaatt tagatcgtga aaaacgatgt tatagttttc ttttagttct
    13801 tttgtcagct ggttgtccat tttccacatt ccttttatgg atattttttc gatacttcct
    13861 tattatactg gttttttgtc aaatttgaaa tggttcggtc aaaaggtaag taaagcttta
    13921 ataatcctag tgattgacga gaagttgtga aaaattcgtt tagtttcgag tatgaagaaa
    13981 aaattttgtg aaaatggctt tccatatttt tgcataaatt tgacttaata ccgagaaact
    14041 ggactttgaa caccgtttat tggagaagtc atgaaatcag cgccttgacc tgaatattag
    14101 agaaataaac agaaaaatat caaaaaaccg aaaaaggaca tcgccctttt tcggtcattt
    14161 ttagtgatta tgaacgtgaa tctgctgtaa cagaaacatt tgcccatggg tttggtacat
    14221 cataagacca atcccagtct ttgacacggt cgttgactgg caatacttca ttacggaaca
    14281 acgttggaat cgcaaatgct tcttcaaatg catattgttg ccatttttga tatgcttctt
    14341 tttggtactc ttcatcaaat gctttgcttg aactgatatc gttgatcaat tttgtatttt
    14401 cttctgattc ataacgagtg tagttgaacg cagcatttgg tccccatagt ccacttggat
    14461 ttggatcagt tcctgtattc caagcagctt ggtaaacatc aatttcagga tcatcgtttt
    14521 ctaatttatc atagaaagaa ttgaattcga tcagacgacc tgttgtgtat tggacatcca
    14581 aaccaatttc tttccattgt tggatatagt aatctgccaa tggttgcgct gtttccccac
    14641 cggacatcga agcaaagttg attgttagtt tatttccatc tttgtcttct cttaaaccat
    14701 cgtcatccac atctttgtag ccagcatcat ctaatagttt tttcgctttg tctagatctt
    14761 gtgtgaatcc tttcatctca tcatcatggt atgactggaa tactggagga atcaatgaag
    14821 tcgcattgct tctcaaacct gcatagaaac gttcacctac tgcattatta tctaatgcat
    14881 agcccattgc ttgacgtaat gatttatcag ccatttttga atctggattg tattccacac
    14941 tctttgtatc tgcattccat ttacctaact tgaatcctag ataagtataa gaagtttgtt
    15001 cacggcctaa gtttgtatag ccagcaattt ctttataggt atcgtaagta tctgttggca
    15061 tttggaaaat catatcatat aatttagagt tcaatgcttc agttgcactt gccatcggta
    15121 cagatttcat cgtgatcttt ttcaatttag gcgtaccatt ccagtagtgc tcgtttggca
    15181 agtattctac tgattctcct gcaacgatat tactcatgta ataagggcca aaagtcactg
    15241 gatttttacg tacttgatcg cttgattcca tatctttgat tgctacacct tctaacgtat
    15301 gttttggtgc tgcatatgac cagataccac cacccgattg aagcatgctt gggtttactt
    15361 ctttatattc gatttcaact gttttgtcgt ctattttttt gattccggag attgtgtcgg
    15421 cacttccatc atgatattct tccataccta cgatgttacg gaaagtgtcg tcataacgga
    15481 taccagtgta atctttattc ccaatgattt cgtaagggaa aatcacatcg tccgccgtta
    15541 catcttcacc atctgaccat ttgacgttgt cttttacttt gattgtcgct tttttcgtat
    15601 cttcatctag atcaagcgtt gcagctccgt cgtctgtgat tctgaaatcg tcatcgtttg
    15661 caaatagcgg ttcatgagaa ggagccataa aagctgcatc atatgaatct tggtaaaact
    15721 cccactggaa caatccttta aactgactgt ccgttgcgac cgcagcttct aattcgccgt
    15781 cttcgattgc atcatcatta ttttttgttg caattggaaa agttaattcc tctttgttat
    15841 tgccgttgcc tccagagttg tttccagagg tatctgtacc tccgcccccg catgctgcaa
    15901 gaacgacagc agacatcagc gctgcaaatc caaaaatcgt tttcttcatt cctatttccc
    15961 ccttcgcttc tatactagcc taaccgttgg cgcgcatctg cagaacgctt gaatgcttgt
    16021 ccaacataat tgatactcaa catcaaaact aaaataagga ttgatgcggg taaccagatc
    16081 cattgccgat tcactaagac ttctccgtta cttgcataac cgatcaatgt acctaaacta
    16141 ggtacatttg ccggcaagcc gaaacctaag aaagtcaaag ttgtctcgat accgatgttt
    16201 gccgcaaagt tcatcgtcaa attagtaatg attaatgaac ttagattcgg catgatttcg
    16261 cggaacatga ttttaaaatc attggttccc attgtttttg aggcactgac ataatctctt
    16321 cttgcttcag ataatgtctt gcttcggaat aatcgggctt tagctaccca ataaaatgca
    16381 ctcatgatga atataaagga ccagacattg tatctggaaa taatcgatac gaagacgata
    16441 atgatcagca tgatcggtag aatcatgata aaatcaacga tccgcatcaa gatgttgtca
    16501 atgatccctc cgtagtagcc agagatgatc cctaatccaa ccccgatgat cgaagtaagg
    16561 atcgtgatcg caaatccgat cgtaacagag tttctagcac ctatgatcaa ttgtcctaat
    16621 acatcacgtc cgccttcatc tgctcccaga atgaactttt gtccttgtgt cgaaactgtt
    16681 cctggtgcag cgtatttgtc gaagatacta acatacatca ctttgtcttg atcggtcagc
    16741 atcgcaccga taaagatcac taataataaa accactagca aacctaatga aaaaatagca
    16801 attttatctt ttttaaattc tcgagcaatc attcgtatcc ccataggagg aatactttct
    16861 tgaactgctg tattgttttt ttcactcatc gatccattct cctttctgat tggttttatt
    16921 gaatccgaat acgcgggtca acgatactca tgatgatatc tgaaagtaat gtccccacta
    16981 gcgtcgcgat tcctaaaatc aatactaagg atgtgatcac ggtatagtcc cgttggccga
    17041 tggaatcaat aaatagtttt ccgactcccg gataaccaaa aatcttttcg ataactacag
    17101 aaccagcaat caacgaagtg atttcatacc ccatttgaga agccatcggc aacgctgcat
    17161 ttcggaaaat atgtcgactg aatactttat tcgtcggtac ccctttggaa cgggctgttc
    17221 tgacaaaatc gagcgatttc gaatcgatta cttcacttcg cagatattgg attgtgacgg
    17281 cagtcgccaa aaatgcttgg gtaattgatg gtaacagaat atatttcaaa cggtctatcc
    17341 attgagtgat aaatccacct tgcaaaccta gtgtttgtga tccacttgtt gggaaccagc
    17401 ctaaacgata accaaaaaca aatagcatga tcaagccgaa gataaataaa ggaacagcaa
    17461 aactaaagaa gttgtagatt acgacaatct tatccaagat tgaattttga taccggccag
    17521 caagcatccc cagaggcaca gcaatcaaat aagtaaggac aactgtcaag atcgatagat
    17581 ataaagtgtt gatgatccgt tcgccgatca agtcagcaac agagcgtttg tataagaaac
    17641 tttgaccaaa atcgccttgc aaagcatttc cgatccaacg gaagtactgg acataccatg
    17701 ggtcattcaa gccagctgct tgtcgcatct gttcgatcac tgccggatcc tgatttggat
    17761 tgatcaaacc ggtaaatgga tcccccggca tcgcttgggc taataagaag atcagaacac
    17821 ttaaaataat aacttgcggg atcatcaata aaacacgtcg cagaatcgtt ttccacatta
    17881 gcttgcacct ccatctttca acgccacttt gtgtgtagtt gtgatcgtac gcaaatcgta
    17941 gactcgacca gtcgcatcat agtattcttt ttggttttgg atatactctt gttctactct
    18001 tcgtcgattt tctttgtggc tttccctatt ttctacatcg atttgcggaa tagctgaaag
    18061 cagacgtttc gtatagatat gtcgcgggtc attataaatg tcttctcttg taccgatttc
    18121 tacaaatcga cctttgtaca taattgcgat attgtcgcac atgtgtttta ctacacctaa
    18181 atcatgtgaa atgaataaat agctgaggcc gtattcttgc tggattcgtt tcatgaaatt
    18241 caatacttgc gcttgaacag atagatccag agcagataca ggttcatcgg ctacgatcag
    18301 ttttggattg gtagcaacag cacgggctac tcctagacgt tgacgttgtc cgcctgaaaa
    18361 ttcatgagga tatttatata gcgcatcttc cggcatccca acgatatcta gcagtccttt
    18421 tacctttttc ttttcttctt gatcgcttaa ccgttcaaaa tttcggatgg gttcagcaat
    18481 gatatccaac actcgctttt taggattcaa actggacatt gaatcttgga agatcatctg
    18541 cacatccttg ttataaccca tttttttgcg gttgcttcgc ttcgttacat cttttccttc
    18601 atacatgatt tgtccggctg ttaccttttc taatcccaca acggcttttc ccgtagtaga
    18661 ttttccagag ccagactctc ccactaaacc atatgtcttt cctttttcga taataaaatc
    18721 cacaccatct acagcatata catgatccgt tactcgattg aagaatccac tgcgaatagg
    18781 gtaatgaact ttcaggtctt tgattgtgat taattctgtc atctacacct ctcctttttc
    18841 gtctctaaag tggaaatgct tatagcagga gcaacgtacc aagtggttcg gtgcgacttc
    18901 atgaaggata ggctcttctt catgatcgct tgctggtatc catggaatac gtggtgcaaa
    18961 ccgacaacct ttacgaggca tgtttttcag agaaggaacg acaccttcga tcacatggag
    19021 ttctgcatca tgatttcctt cttgtggtat agagttcagt aaagagcgtg tataaggatg
    19081 cttaggatga tcaaacagtt cttttacacc tgctacttca acgaactgtc cgccatacat
    19141 cactgccact cgatcagcca tttctgctac gactcctaag tcatgagtga tcaagatgat
    19201 ccctgttttt gtttcttcct gaaggtcttt cagtaaatca agaatctgtg cttggatggt
    19261 tacatctagg gctgtcgtcg gttcatctgc aatgacgatt ggaggtttgc aggcgattgc
    19321 gattgcgatg atgacacgtt gtctcatacc gcctgatagt tcatggggat attggcgtcc
    19381 aactctcgca ggattaggaa tccctacttg ttctagcaat tctagtgcac gagcttgacg
    19441 ctgttctgca ttcatctttg tgtgatagga aagtccttct ttgatctggt cttcgattct
    19501 catgagagga tttaatgcag acaacggatc ttggaaaatc attccgatat catttccgcg
    19561 gattttatta tagagcgttt cgttcaggtc gattaaattc agatcgttat ataaaatctc
    19621 acctgtaatt tttgtgttat taggatcaag taatcccata atcgtagtag ccaaggtact
    19681 tttcccgcac ccagattccc caacgattgc taaaatctcg tttttgtcta aagtaaatga
    19741 aacgtcatct actgcatcgt aatagtcatc tttgagtcgg aatcctgtat gtaggttctg
    19801 aacttctaat aattggttat cggacaatcg aatacccctc tctgcttcct aaatggaagt
    19861 ttttcttaat aaaaaaggac attacacaga ataaatcgtt ttataacaaa ggttat
//
LOCUS       DAZULY010000020        19521 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000020 DAZULY010000000
VERSION     DAZULY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.020"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..751
                     /locus_tag="ACG81A_000535"
     CDS             68..751
                     /locus_tag="ACG81A_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFS5013929.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="ACG81A_000536"
     CDS             753..1589
                     /locus_tag="ACG81A_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS5013930.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="ACG81A_000537"
     CDS             1600..3354
                     /locus_tag="ACG81A_000537"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5013931.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="ACG81A_000538"
     CDS             complement(3630..3971)
                     /locus_tag="ACG81A_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS5013932.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="ACG81A_000539"
     CDS             complement(4039..6210)
                     /locus_tag="ACG81A_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS5013933.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6421..7278
                     /locus_tag="ACG81A_000540"
     CDS             6421..7278
                     /locus_tag="ACG81A_000540"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFS5013934.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="ACG81A_000541"
     CDS             complement(7349..8206)
                     /locus_tag="ACG81A_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5013935.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="ACG81A_000542"
     CDS             complement(8191..10893)
                     /locus_tag="ACG81A_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS5013936.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="ACG81A_000543"
     CDS             complement(10906..12195)
                     /locus_tag="ACG81A_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFS5013937.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12345..13391
                     /locus_tag="ACG81A_000544"
     CDS             12345..13391
                     /locus_tag="ACG81A_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5013938.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13393..15546
                     /locus_tag="ACG81A_000545"
     CDS             13393..15546
                     /locus_tag="ACG81A_000545"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFS5013939.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(16019..17470)
                     /locus_tag="ACG81A_000546"
     CDS             complement(16019..17470)
                     /locus_tag="ACG81A_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5013940.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(17467..19191)
                     /locus_tag="ACG81A_000547"
     CDS             complement(17467..19191)
                     /locus_tag="ACG81A_000547"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5013941.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(19184..>19521)
                     /locus_tag="ACG81A_000548"
     CDS             complement(19184..>19521)
                     /locus_tag="ACG81A_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5013942.1"
                     /translation="SVQIKGLIFLAIDFILLFALLCLFATYQYSMILDSEYTISVPNL
                     LKLSFISVFSSFSSFLKIIIGSGIILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREE
                     LDKQLESYE"
BASE COUNT         5526 a         4029 c         3520 g         6446 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa agattatact
    12241 tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact ttttcatcca
    12301 ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgact caaccattgt
    12361 accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta cctgtagaca
    12421 gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga atcacttcca
    12481 aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc gggaaaggca
    12541 gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa acacgacgga
    12601 ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc aagggattga
    12661 accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac tttctacaaa
    12721 ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat gctcttgata
    12781 cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct gtcgtcatat
    12841 tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat ttccaaggcg
    12901 gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct tatttgtttg
    12961 gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt gaagcattgg
    13021 acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca ttagatgaat
    13081 tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac gatgttgtcg
    13141 ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa gatttttcaa
    13201 ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg acaaccatcg
    13261 cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact tggttagaga
    13321 caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga caatcaacaa
    13381 aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc gaatcagcca
    13441 aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat gaattatttt
    13501 gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga ccgcgttttt
    13561 caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag tcatatgctg
    13621 tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca aagttcttac
    13681 cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt acgttatggc
    13741 atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga ttacagcaaa
    13801 aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt tatagatcct
    13861 tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga tttttctttt
    13921 tattttatat tgagatttga atatccttta acaattgatg agttagctgt ttccaacaaa
    13981 aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat ccgatttggg
    14041 acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca aaaccaaagc
    14101 aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga tcgtatacag
    14161 ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata tcgggtgttt
    14221 ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca ctatgatacg
    14281 actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg ggatacgtat
    14341 aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga aatgctggaa
    14401 ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc tcctgatgaa
    14461 cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc tgtaaaagat
    14521 atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc tacggttcaa
    14581 agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata tggctatgtt
    14641 cctaatcact accacgaatc agtcaatcac acattagact atgcttatag cgatttttgt
    14701 attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata tcagcagcaa
    14761 tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc aaaagataaa
    14821 gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta tgcagaagga
    14881 agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat cggaaaatat
    14941 ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc cccaacgttt
    15001 gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc gatagaattt
    15061 ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt cagttatata
    15121 ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac cttcgacgat
    15181 tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg gtatatcttc
    15241 aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat cggtatgcca
    15301 ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat tgaagcaacg
    15361 cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacag agaatatacg
    15421 aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta ccaattaggg
    15481 atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt atcaaactat
    15541 aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa
    15601 aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa gcttttgaac
    15661 accgtttatt gagaggttgt gaaatcagcg ttctgacctg agtattagag agacaaatgg
    15721 caaaatagtt cctcatattt tgccattttt tgagctaata ccgagggtca gctgattgaa
    15781 caccgtttag tggagaggtc gtgaaaaagc tgtttagttt cgagtattaa gaaaaatttt
    15841 gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgagaa actggctttt
    15901 gaactccgtt tatcgagagg tcatgaaaaa gctgccttgc ctcaagtaat aagaacagcc
    15961 caaaattaga tttctagaca attttttata agttatttca ttacattggc tttttctttt
    16021 atgattctcc actgttttct cggtactctt taggggatct gccgacgatt ttcttgaata
    16081 atttagagaa atacgtggtg ttgttgtagc caatctcact tgctatctca ttgatgtttt
    16141 gattactgtg caaaagcagt tgtttcgctt tttccatacg taaatggttc aaataagctg
    16201 aaaaactttt tttcgtttct tttttgaaca gctgtcctag atacatgaca tttaaatgca
    16261 ggttttctga cacttctttg agtgtaagtg gttcttgata atgctggtgc aggattacga
    16321 ttactttttc cacattgtca ctgtactttt tctgtctctg atgctttttg atgaatgaca
    16381 gaagtagctg ctgaagttct tgcaccgttt ttgcctgatg gattttttcg attccttgta
    16441 aatattcctc atcttccaga tcaatcaatt cccgttgaat ctccataaac agtaaaaaac
    16501 tggaatatcg tatatcttcc ggcatcatgg ctgctaactg aaactgttca aaaaaatcag
    16561 tgatcatttt ttgagcgact tccaattgtt tattttgcag cacgcgtctg aaacttgaaa
    16621 aatcgatggt ttgatctttt gtttctgctt gtactgcgta aaaaacatgc tcgtctttat
    16681 ctccataaaa ttgatgcaac tgtagattgt ctttcgcttg ttggaatgtt tcagggattt
    16741 tgtccaaaga atctgtctct tccccgatac tgatcagcca ttcctgatca ggaaagtttt
    16801 cttgaacaaa acaacagaag ccatcaactt catctgcctc caacaacaac gtgatcaata
    16861 cacggtctcc gtaatttcgt tgatagtaaa agatttgttg ccgttcttgc aaccatctat
    16921 tgataagagt atcatgagtt cttgccagtt gaatcaatac tatcctatgc tgcttctcac
    16981 ttatttgtcc tattatttct tcttcactta tttcatccaa ttcttcattc aaccattgac
    17041 tgaataacag ttcttgatag atttcttgtt gatttttttg ttcattttgc tgatctaacc
    17101 gtgttttgat tttcttgagg ctttcaacca attcgaactt attcacgggt ttcatcaaat
    17161 aattcacggc gcctaattgc atacctcctt tcaaataatc gaattcttgg tatcctgata
    17221 aaatcataaa ttcaaaatta tgacgttctt tttgagccgc ttcaataaat tccagtccat
    17281 ttatttctgg cattgtcaca tccgttacta ctaaatcaat ctgtcgctgt tcaagtacag
    17341 ctagtccttc tttggcattc tctgctgttg ctactatttg aaagcctaaa ctttgccaat
    17401 caattatctt ttttaaccca ttcaaaatca tgtattcatc atctaccaat aacacttgat
    17461 acacgctcat ccctccttga atgtaataga aacggtcagt ccacctgtct cattattctc
    17521 aagcgacata taataactgc tgccgaagct ggcacgtaaa cgttcattca cgttttgcag
    17581 accgatagaa ccgtgaagtt ccactttagg atgagacagt ttttcttcaa tctgttttag
    17641 ccgtttttca gtcatccctt ttccattatc tttgatgatg atccttaccc gtttgccttc
    17701 ctgcaagact ttgacactta gcgcattatc gaatcgagta aaatcaatac cgtgtttaaa
    17761 ataattttca actaatggct ggatcacaaa tttaggtact tcgatctttt ccaaatcagg
    17821 atcgatcata aaatgatagg ctactcgatt aggataacgc atttgataca aatagacata
    17881 tttttcgcaa aagtccagtt cttcttttaa agtgatcgtt ttctcttgat tcgtattgtt
    17941 tcgaagcaaa gcggaaaacg catacacaac atctgctaat tcttcacttc cttcgctgat
    18001 tgcatacatt cggatatatt ctaatgtatt atataaaaag tgtggattga tctgtgcttg
    18061 aagcgcccgc atatgtgcgt cttgctgttt gatttctaat ttgtagatgt cttctacaaa
    18121 ctgattgatg ctatccaaca tttcgttgat accgattgaa agttccctca gttcatattc
    18181 cgtgtttgtt atgtcgatcc gcgtctctaa attccctgct gctgtttgat ccatggcctg
    18241 catgacgata gatacttgct gtgaatatcg tttgaatgtt ttataaagaa agataagcag
    18301 tatattgtct aaaataatga taccaatgat cagcggggca agattttttc ccgtttcgat
    18361 ttttacactt tttttatcta gtacagctaa tatacgataa tcatcagaca gttcttggtg
    18421 ttgaacaaaa ttgttttttt ccagatcgtc aatcggcaat tgtgaatttt tcttcagact
    18481 ctctgtgatg atcatttctt gtctttcaaa cgtttcttta gctattttat tatcatggaa
    18541 tgtgtatagt cgatttgtcg ttccagaaat catgtataaa gagagtccat tgaatgacgt
    18601 tagattttct aaaatcatat ctagttcttt cttttgaaaa ccaataaaaa tacttcctga
    18661 ctcagctcct ccttgtgtga taggcaactt gatataaaag gcgtctagta attttggtgt
    18721 cccctttttt atttccccac ctttgttttc tctagaagat tcataatact ccggtagttg
    18781 attcaacacg atatatatcg cacttagttc ttcataattt gcgtagagat ctttgatttg
    18841 atttgggaaa gaaactaatt catcggtact gttttgctgt tcgtatacat acatcagata
    18901 ctgatctatg ggttttgtta agtaggtcgt cacattttcg atcttttcgg aattccctac
    18961 aagctggttt ttgatgatcc tactcaaggt tgttttatca ttcagcattc tgcttgtgct
    19021 ttgaacggca tcacttgtca tgttttctgc ctgtttttga ttttgttccc aagtatggat
    19081 actaaaaata acggtagcag tcatcgttac accgatcatg ataaccgcgt atttccgcaa
    19141 cagacgattt agtaaggttc cttttgaaaa tagaatccga aacttattca taactttcca
    19201 actgtttatc taattcttca cgccaatgtg ttgtcatcgt accattccaa accgtcaaaa
    19261 gaccaatgac accaaacagg atcaatcctt tgaattgcca agtcacgcct aaaattatac
    19321 cgctaccaat aattattttt agaaaagacg aaaagctgga aaaaaccgaa ataaaactta
    19381 gctttagcag attaggaaca gagatcgtat attcactatc taaaatcata ctgtattgat
    19441 aagtagcaaa taggcagagc aaggcaaaaa gtaaaataaa atcaatagct aaaaaaatga
    19501 gccctttgat ttgaacagat a
//
LOCUS       DAZULY010000021        19401 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000021 DAZULY010000000
VERSION     DAZULY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.021"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            374..1063
                     /locus_tag="ACG81A_000549"
     CDS             374..1063
                     /locus_tag="ACG81A_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5013943.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1050..2258
                     /locus_tag="ACG81A_000550"
     CDS             1050..2258
                     /locus_tag="ACG81A_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS5013944.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2552..3823
                     /gene="serS"
                     /locus_tag="ACG81A_000551"
     CDS             2552..3823
                     /gene="serS"
                     /locus_tag="ACG81A_000551"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFS5013945.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4372..5856)
                     /gene="guaB"
                     /locus_tag="ACG81A_000552"
     CDS             complement(4372..5856)
                     /gene="guaB"
                     /locus_tag="ACG81A_000552"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFS5013946.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6361..7056)
                     /locus_tag="ACG81A_000553"
     CDS             complement(6361..7056)
                     /locus_tag="ACG81A_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFS5013947.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7146..8246)
                     /gene="ychF"
                     /locus_tag="ACG81A_000554"
     CDS             complement(7146..8246)
                     /gene="ychF"
                     /locus_tag="ACG81A_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFS5013948.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8255..8446)
                     /locus_tag="ACG81A_000555"
     CDS             complement(8255..8446)
                     /locus_tag="ACG81A_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFS5013949.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8467..9357)
                     /locus_tag="ACG81A_000556"
     CDS             complement(8467..9357)
                     /locus_tag="ACG81A_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFS5013950.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9344..10111)
                     /locus_tag="ACG81A_000557"
     CDS             complement(9344..10111)
                     /locus_tag="ACG81A_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS5013951.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10123..10836)
                     /gene="rsmG"
                     /locus_tag="ACG81A_000558"
     CDS             complement(10123..10836)
                     /gene="rsmG"
                     /locus_tag="ACG81A_000558"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFS5013952.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11068..12969)
                     /gene="mnmG"
                     /locus_tag="ACG81A_000559"
     CDS             complement(11068..12969)
                     /gene="mnmG"
                     /locus_tag="ACG81A_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFS5013953.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12986..14383)
                     /gene="mnmE"
                     /locus_tag="ACG81A_000560"
     CDS             complement(12986..14383)
                     /gene="mnmE"
                     /locus_tag="ACG81A_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFS5013954.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14682..15065
                     /locus_tag="ACG81A_000561"
     CDS             14682..15065
                     /locus_tag="ACG81A_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013955.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15233..15991)
                     /gene="jag"
                     /locus_tag="ACG81A_000562"
     CDS             complement(15233..15991)
                     /gene="jag"
                     /locus_tag="ACG81A_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFS5013956.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16009..16827)
                     /locus_tag="ACG81A_000563"
     CDS             complement(16009..16827)
                     /locus_tag="ACG81A_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFS5013957.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16824..17180)
                     /gene="rnpA"
                     /locus_tag="ACG81A_000564"
     CDS             complement(16824..17180)
                     /gene="rnpA"
                     /locus_tag="ACG81A_000564"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS5013958.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17298..17432)
                     /gene="rpmH"
                     /locus_tag="ACG81A_000565"
     CDS             complement(17298..17432)
                     /gene="rpmH"
                     /locus_tag="ACG81A_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFS5013959.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17955..19289
                     /gene="dnaA"
                     /locus_tag="ACG81A_000566"
     CDS             17955..19289
                     /gene="dnaA"
                     /locus_tag="ACG81A_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFS5013960.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
BASE COUNT         5501 a         3988 c         3572 g         6340 t
ORIGIN      
        1 actcagttat cttatcatgt catttccaac atgtcaacaa cttatttttt attatttaat
       61 aagttattgg tgttcttttg aacaaataat aatatatcaa catcacatgt gaaagtcaat
      121 aagaaaatgc ttatttatca acatttctag cttacatgat cattggttag ttctggtcct
      181 ttttcgttcg tctttataac tccgagagac gtaaaatcct tttgttttca gtttaatgtt
      241 catgttcttg tcttattcac accaactaga tgtctcacct tctaaaattt tccttaacaa
      301 agtgctgctt ttatctattt ctgctttttt ttgctagaat aatagtaaca atatgactct
      361 ggaggattta tttatgaaaa ttttgattgc tgacgatgat aaagaaatcg ttgaattatt
      421 aagtatctat gttcacaatg aggggtatga agcagtaaag gcttatgatg ggaaagaagc
      481 attatctaaa atcagaacaa tccctgatat tgaattactt attttagata tcatgatgcc
      541 tgagatggat gggatgcaag tagtgaaaga gctacgaaaa gaatcccaaa taccaatcat
      601 tatgctgaca gcaaaaacta ctgatatgga taagatcaaa ggactggtcg ctggtgcaga
      661 tgattatgtg accaagccat ttaatccgtt agaagtaatg gctagagtaa agtctttatt
      721 acggcgtaca aaaatgcaga ttgccccaga tcagcctgat gtattagaag taaatgcatt
      781 aatcatcaat aaagactctc atgaagttaa aacaatcgat gggaaagaaa tccagttaac
      841 agctttggaa ttcggtatcc tctacttgct tgctagtcat cctaatcgag tatttagcgc
      901 ggatgaaatt tttgagcgtg tttggaaaca agaaagtatt gtctctgcta aaactgtcat
      961 ggttcacgtt agtcatttaa gagataagat tgaagaagca actggtggtg aaaaagtcat
     1021 tcaaaccgtt tggggagtgg gctataaaat tgacgcaaga tagtaaacga gcaactgaaa
     1081 aacgccgtcg gatcaatttg acttcaaaag aaatcagcga attgttagcg gaaggaatca
     1141 tcacgattat ccttttgctt ctattaaatg tttctattct ggttgttatc agttcggtga
     1201 tcaacagttc accttctttg acgaatgcga tttgggattc aaagaatatt tttgcagaac
     1261 gattgaacac tgatttgttc tggaacggcc ggaattttat tattcccttt ttctttctat
     1321 tagatattgg tgttttgtat tggcgcttga ttcgaagata tcgccaaatg caattgcggc
     1381 atatcatcag cgaacttcac tacatcgcaa acggaaatta tgatcatcgt atcccttttg
     1441 aactgagtgg tgacttaagc cgagtggtaa caagtatcaa tggattggtg gatagtaccg
     1501 tagcagcgat tgaagacgaa cgtaaaatcg aaaaatcaaa agatgaattg atcacgaatg
     1561 tcagtcatga tatccgtacc ccactcacgt ccatcattgg atacctagga ctgatagaag
     1621 atggccagta tcactcagaa gaagatcttt taaagtatac gcatactgct tatatcaaag
     1681 caaagcagat gaaatcttta gtggacgact tatttgaata cactaaggta cgccagcctg
     1741 ctgtcccggt gaatttttct gcctttgaca tgatccagtt gatcgaacag ttagcagctg
     1801 attttgagtt ggaagcttct aaaaaaaata tccaaatact tgttcaatca aaagtagact
     1861 cccttatcat ggatggcgat accgaaaaac ttgtacgtgt cttcaataat cttttgacga
     1921 atgcgttgaa atacggaaaa ggagcaacga agatcgtaat cgaagtagaa cgtatcggtt
     1981 cagaagttgt tgcaacagtc aaaaataacg gagcaatgat cccacaacag gcaatcgata
     2041 atctattcga ccgtttttat cgtgtagaag aatctcgttc acaagcaact ggaggaactg
     2101 gacttggttt agcaatcgct caaagcattg tggctttgca cggcggatac atttatgcaa
     2161 aatcagataa gcaatggact tctttcatca tgcaccttcc aatcaagaaa aatgaaaaat
     2221 tacctattga tacacaggaa gttattgacg aaagctgaaa tattcgctat tcttattaca
     2281 attattagaa taaatgaaaa acacagaacc cagtacactt tattcttttt cagagagctg
     2341 atggcaggtg taaatcagca aaggataatt tggaagttcc agttcgttta cgtgcccttt
     2401 cgaaataaga aaggtcggtt caacagccgt tatgtgttca acaagtgcag ctgacgctga
     2461 acttgggtgg taccgcgaat ttcagactga ttcgtcccaa gaataatggg atggatcagt
     2521 ctttttttat tttcaaaaag gaggaaatca tatgttagat gtaaaaatga tgcgacaaaa
     2581 tattgaagaa gtacaagaaa aattgatgac tcggggcgta aaaaaagaaa cattagctga
     2641 attcatggaa ctagatgaaa atcgtcgtaa gctactagtc aaaagtgaag aattaaaaaa
     2701 atatcgaaat gatgtttctg ctgagatcgc ccaattgaag cgtgcaaaag aagatgcttc
     2761 tgctaaaatc gctgaaatga aagaagtcgg tggcaacatc aaagcattgg atactgaaat
     2821 tgctgaagtg gatgaaaaat tgaaacaaat tgcaactact ttgccaaacc ttccccatga
     2881 ttctgtgcca gttggaacag atgaagagga caatgtagaa atccgtcgtt ggagcgaacc
     2941 acgtacattt gcttttgaac caaaaccaca ctgggaaatt gcagaaaatc ttgatatttt
     3001 agattttgaa cgtggcgcaa aagtagccgg tagccgtttt gtctattaca aaggattagg
     3061 tgctcgatta gaacgtgctg tctacaactt catgcttgat cagcatattt acgaacacgg
     3121 ctatactgaa atgatcactc cttatatcgt caacagtaaa gcgatgtttg gtacgggaca
     3181 atttccaaaa ttcaaagaag atgttttcca attggagaat actgatctaa cattaatccc
     3241 aacagctgaa gtaccattaa ctaactatta taatgatgaa attttagatg gtgcagattt
     3301 acctatttat ttcactgcac taagccctgc ttttcgttct gaagccggaa gtgctggtcg
     3361 ggatacacga ggactgattc gtcttcacca attcaataaa gtagaaatgg tcaaattttc
     3421 ggatgtagag cattcttacg aagaattgga aaaaatgaca gctgatgcag aagatatctt
     3481 gaaaaaattg catttgcctt atcgtgtgat ggccttatca actggagaca tgggcttttc
     3541 tgcagctaaa acttatgact tagaagtatg gatacctgca caagatactt atcgtgaaat
     3601 cagttcttgt tctaactgtg aagatttcca agcacgccgc gcaatgatcc gttatcgcga
     3661 taatgatgga aaaatccact atactcatac tctgaatggt tcaggacttg cagttggccg
     3721 tacagtagct gccattttgg aaaactacca aaatgaagat ggttctgtca cagttccaga
     3781 agcgctcgtt ccttatatgg ggaatctgaa agttattaaa taaagaggct gtgaaaaagc
     3841 cgcttagttt cgagtattaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa
     3901 attggactta ataccgagaa actggctttt gaacaccatt tatcgagagg ttgtgaaatc
     3961 agcgaattaa cctgagtatt agagaaaaaa atggaaaaat agtccctcat attttaccgt
     4021 ttgttgaact aataccgagg gtcagctgat tgaacaccgt ttagtggaga ggtcgtaagc
     4081 ctacccccca gctcctagtt acaaggacaa attcaaaaaa cagctcttca tattttatga
     4141 atgttgtact tgtgattcgg aggtggcaag ggaacactgt ttattcaaaa agaggctggg
     4201 acagaagcgt ttaactccaa gaaataagaa gaaattcacg aaaattgctt ttcaaatttt
     4261 tgtgaatttc ttcttatttc cgaaggagtt gcttctactt ccgccgttta tacgtgttta
     4321 gagcgtgaga caaaagtgtt ctttactttt gtcccacgct ctttaatgtt tttaagattc
     4381 aactgaatag tttggtgctt cttttgtgat ttgtacatca tgagggtgtg attcttttaa
     4441 tccactgccg ctcatttgga taaattgcgc ttcatcacgt aattgctgta gattggctgc
     4501 cccaacataa cccattcctg aacgcaaacc gccgatcatt tggaaaataa tatctgcaac
     4561 acttccttta taagcaaccc gaccttcgat tccttctggt actaatttat ttgcttcatt
     4621 gacactacct tggaagtaac ggtcttttga acctttttcc atcgcgccta atgagcccat
     4681 acctcggtat gttttgaatc gacgtccttg gaagatttcg aattcacctg gtgattcatc
     4741 tgtccctgcc aacatacttc ctagcatcac tgcatgtccg ccggcagcta atgctttgac
     4801 gatgtcacca gaatatttga tccctccgtc tgcaataatc gctttgccat attgacgagc
     4861 aactgatgct gcatcataaa tagctgtgag ctgaggtaca cctacaccgg ctaccacacg
     4921 tgtcgtacaa atcgatcctg gtccgatacc aacttttacg acatctacac ctgcatcata
     4981 taaagctttt gtcgcttcag ccgtcgcaac gtttcctgca atcaatgttg catctgcaaa
     5041 agttgaacgg atttcttgaa tttttcggat cacacctgca ctatgtccat gagctgtatc
     5101 gatgatgatt gcatctgccc ctgcttctaa taaagcattc gcacgctcaa atgtatcgct
     5161 tgttacacca acggctgctg ctacaagcaa tcgaccatgt tcatcttttg ctgcatttgg
     5221 aaattcgatc actttctcga tgtctttgat cgtgatcaat ccgcttaaac gaccttcgtt
     5281 atctacgatt ggtaattttt cgattttatg tttttgcaaa atcttttctg catcttttag
     5341 agaggtacct actggggcag tcactaagtt gtcttttgtc atcacatcag caatcggcat
     5401 gtgataatct gttacaaaac gcatgtcgcg gtttgtaatt atccctacca attttctgtt
     5461 ttcaagtgtt tcaacgattg gcacaccact gatacgatat ttgctcatca attcttctgc
     5521 gtctgctaca agatgctgtg gcgttaagaa gaacggatca atgattacgc cactttcaga
     5581 acgtttgact tttcttactt catccgcttg ctggctgatc gtcatgtttt tatggatcac
     5641 acctagtccg ccttgtcttg ccatagcaat ggccatcttg ctgtcggtaa cagtatccat
     5701 acttgcactg atgattggaa tattcaactt aatattttta gctaattgta cgctcatatc
     5761 cacttcgttt ggtaacacgt gactttctgc tggtatcaat aagacatcat caaaagtaaa
     5821 tccttttttc gtgaactttg tttcccagtt ggacattttt agtaccactc ctctaattta
     5881 gtttatctat taaaataatt gattttttca tttaagtcaa tgatctactt ggaatcaagt
     5941 tagctttctc atagaatacc aatataaagc gtttacatct gtgtatatta aaagagattg
     6001 taaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc ttctcatgtt
     6061 tttgaattgg ttgggtttat taccgcagat gcaaactttt gaacaccgtt tagtggagag
     6121 gctgtgaaaa agctgcttag tttcgagtag taagaaaaat tttgtgaaaa tggctttcca
     6181 catttttgca taaattggac ttaataccga gaaactggct tttgaacacc gtttatcgta
     6241 aaaagggact gtgacaagac ttttgtcaca gtcccttact attatttaac tatggataaa
     6301 caaaatgagg ttctttacta cccctagata tctagcaaaa tagctgatct ttttgctagt
     6361 ttatcttctt gggacggata aacttccaat aatcccgtat tttttcttca agtagtaacg
     6421 gattgctaaa gcagttgccc cgataatgat aactactact ggatctaata ctggattgat
     6481 aactggtggc aatagtgttg tcgcagaaaa cagtacgacc caaagaagtg tcgttccacc
     6541 taggataagc aatgtcttac ccattcctgg tttcttgctt cgatctgctc cgggatattc
     6601 atattgatag atgtatttgt acatcatata aaagacccag ccaccgattg cagaacctag
     6661 aacgagtgta gaaaagccat aagctggcgt tgttccacgt gaaaacaatc cggtaatcgc
     6721 aatcatgata cttaatagac caagcaacag caacgtatta tctaaccaca tcaataaagg
     6781 ggtggatgaa ttttttactt ctgctggctt attgatgatt gcttccgttc tttcagagac
     6841 tgtcccaaac aattgacggg ctgttcttcc gcctttttgt tctgcaacta aagtaggcag
     6901 aatgtcatga agggctttta ctttttcttc ttcagacaaa ttcgctgctt ctaaagattt
     6961 tttcaaatca aaaatgtact gttgatttct tttggttaat tttgtttcta aactattgtt
     7021 ttcttttacg attgctcgta attgttctgg ttccattatc taatagtcct ccttagccgt
     7081 tccttttctc aacggtcgtc acaaaagaca ttctaccata ttctacagct agtttgatag
     7141 cccttttaca cattaaagcg gaataacatt acgtcgccgt cttgtacgac ataatctttt
     7201 ccttctaatc gaacacgtcc agcttctttt gctgcatgca tgtttccgta atgatccaag
     7261 tcttcaaaag agacagtttc tgcacggata aatccgcgtt cgaaatctga atggatgatt
     7321 cctgctgctt gtggtgcttt gattcctttt ttgaatgtcc aagcacgtac ttcttgttcc
     7381 ccagcagtaa agtaagtagc taagcctaac aaatcataag cagcacggat caactgatcc
     7441 aaacctgatt cttcgattcc taaagcttct aaaaattccg ctttgtcttc ttcatctaat
     7501 tctgcgattt cttcttctgc acgagcactg accacgatca cttctgcatt ttcttctgca
     7561 gcaaagttgc gcacttcttg tacataatga ttcgcgtctg catctgctac gtcatcttct
     7621 gagacattgg ctacataaag aattggtttt gtcgtcaata aaaatagtga tttaacgatt
     7681 ttttgttctt cttcagtaaa ttcgatcgtt cgggcagatt ttccttcttc cagtacaggt
     7741 ttgattttat ccagtacagc tagttctgcc actgcatctt tatctttggt tttagcaact
     7801 tttgctacac gagtatagcg tttgttgata gattctaagt ctgctaacac tagttccaag
     7861 ttgatcgtat caatatcttc tagtggatca acacgtcctt ctacgtgagt aatattatca
     7921 tcatcaaaac agcgaacaac gtgacaaata gcgtctactt ggcggatatg gcttaagaat
     7981 tgatttccca aaccttctcc tttacttgcg ccttttacga ttccagcaat atctgtaaat
     8041 tcaaaagtcg tcggaacagt ttttttcgga tgtacaagtt ctgttaagcg ttgcaaacgc
     8101 cagtcaggta cttctaccat ccctacgttg gggtcgatcg tcgcaaaggg atagtttgct
     8161 gcttctgctc ctgcttttgt gatcgcatta aacaaagttg attttcctac gttgggcaac
     8221 ccaacaattc cagcagtcaa tgccataaat ttattcactc tttctctgca tttttttcaa
     8281 ttactttttt taatttcttt tcaaaatctc gacgggtcat catgacgata tgtccgcaat
     8341 tcatacatct gattttgata tctgcgccca ttcgaatgac ttcccagcga ttcgtctggc
     8401 aagcgtgagg ttttttcatt tcaacgatat cgtgcaagtc gtacataaaa atagcctcct
     8461 ttttgtttat tcttcatcaa attgaatatt caagatatca agaattcgag tcaaatcttt
     8521 ttgagaaaga tattcaatct cgatcttacc tttcccattt ttttcttgga tagctacact
     8581 tgtaccaaat ttatccatca aacgatcttc gctttctcgg atataatatg gtttttcctt
     8641 tgctagcctt gggatttttt tcttttcttt tccatcattt tgatttaact cagaaacaag
     8701 ctgttccagt tggcggacag ttaaattttc agttactgca cgattggcta atttcaagat
     8761 caatgtttta ttttttaaac ctaacagcgt acgggcttgt cccatagata aacgctgatc
     8821 ttgtaccatt tccttgacaa gatccggtaa agaaagcaag cgcaaataat tggcgatata
     8881 aggtctgctc tttcctaaac gactagctac ttctgcttgt gttagtttca ggtttttcat
     8941 cagcatctca tatgcttctg cttcttctaa cggattcaga tcttctcgct gcaagttttc
     9001 cagcactgct acttgcatca tcgcttcttc gtcaaagtct ctaacgatag cagggatcgt
     9061 ttctttttca gctagtttgg acgctcgaaa acgtcgctcc cctgcaataa tctcatatcc
     9121 ttttactgcg gaacggcgta cgatgatggg ttgaaagaca cctgattgtt caatagaatt
     9181 agctagttcc tgcaaagata cttcttcaaa tgttttacgg ggctgatacg gatttggtcg
     9241 cagctcattc aatggtagct ggacaacggt atcatctttt acatccaccg tctctaagtt
     9301 ttctaaatct tgaaacagtg catcgattcc tcggcctaat cctttacttt tcttcacgac
     9361 ttaccacttc ctttgccaat gcttggtaga cttcagctcc tcgagaacgt ggatcgtagt
     9421 cgatgatcgg ttttccatga ctaggtgctt ctgataaacg aatatttctt ggaatgatcg
     9481 tttcgtaaac tttttcgcgg aagtatttac gtacttcttc tacgacttca tttcccagat
     9541 ttgttcgtgc atcatacatt gtcagtaaga ctccctcgat ctctaattct ggattaaagt
     9601 gtttttggac taaacgaacc gtattcagta attggcttaa tccttccaat gcgtaatact
     9661 cacactgaac aggtatcaaa atcgaatcac ttgctgtaaa tgaattgatt gtcagatggc
     9721 caagagaagg cgggcaatcg atcaaaatat aatcatactg actgcttact tctgctaaag
     9781 atccttttaa tcttgattct cttgccatca ttgacgtcaa ttcgatctct gctcctgcta
     9841 attggagagt cgcaggcaca atactcaaat tttcgtgttc agtgatcaaa gttgcctcat
     9901 caattggcaa ttcattgact agaacatcat agatgtctct tgtcacatca ggttttctga
     9961 tcccgacacc actggtagca tttccttgtg catccatatc cactagcaat acttttttgc
    10021 caaggctagc aagacaagca cctagattca ccgtcgtcgt tgttttgccg acgccacctt
    10081 tttggttcgc aacagatatt atttgtgcca tctcttctcc tcctatttga ttggctgctt
    10141 attaggcagt ccgggctttc ttgggtattt ctttggggtt ggtttctttt tttgaataac
    10201 gatgatatgc cgttgatcag agacattcgg caaggtaacc gcctcttctt tgaggaattt
    10261 accacctaat aaagcaatcg cttgttttgc ttctgtcaat tcctcttcac tttttgctgc
    10321 tttcaatgca taaaaatagc cttcttgttt agccaagggc aaacacaatt ccgctaacac
    10381 attcaaacga gcaaccgcac gagcagtgac aaggtcaaat gatgcacgaa agtgaggatt
    10441 ttgcccaaat gtttctgccc ggtcatgata taaggaagca tcgattccta ataatcgggt
    10501 taactcagat aagaaggtta ttcgtttatt cagagaatca acgatcgtta tctgcagttc
    10561 tggaaaggca atttttaaag gtatgctagg aaaccctgcc cccgaaccaa catcgcataa
    10621 agataaaggt tgcgtaaagt ctacttcaaa tgctaatgta agtgaatcgt aaaaatgctt
    10681 cagatatacg tctttttcat ccgtaatagc tgtaaggttc atcttttcgt tccattcgac
    10741 taatgtccga taataaatag cgaattgatt catttgatga tcggtaagct ctatcccttt
    10801 ttctgccaag gattggcgaa attcttctgg tatcataaat attcctttca ttgtgaaaca
    10861 gcaattgttt cacagattct ctccctactt taacgcaaaa tctctagaca tgcaatcctt
    10921 ccatatggat tcttaaaaat ttgaaaagta agaaaagccg atagactttt tctttctagt
    10981 atgactcata aaatatacac agaagagaga actagctttg agcaatttct gcaaaaacta
    11041 gttctctctt ctatttatta ttacgtatta cgctggttca ctgactttcg caatcttccc
    11101 ttgttcgata tagaccatta ggatacttag gtctgcaggg tttacgccac tgattcggct
    11161 agcttgggca attgtttctg gttggatttt ctgcaatttt tgttttgctt ctgttgctaa
    11221 tccgttgatt gctgcgtagt cgatgttttc tggaatcttt ttcgcttcca tgcgttttaa
    11281 tttttctact ttttccatgg cttttttgat gtagccttca tatttgattt ggatttctac
    11341 ttgttcgatt tcttttcgtg tcaattcttc attttctgga ataaaccgta gaagttctgc
    11401 ataagtaatc tcaggacgtc ttaaaaagtc gcttgctagt acaccatctt ttaatggtgc
    11461 cgcattaatc gttgctaaaa agtcttggac ttcttctgtt ggtttgattc gtacattcat
    11521 caaacgtttg atttcttgtt caactgcttc ttttttcttc aaataattct tgtattgatc
    11581 ttcttttact aagcctacac ggtgacccat ttctgttaat cgcaaatctg cattatcatg
    11641 acgtaaaagc agacggtatt cagcacgtga agtcagcaaa cgataaggtt cgttcgttcc
    11701 ttttgttacc aaatcatcga tcatgacacc gatgtaaccg tcacttcgtt tgatcactaa
    11761 aggttctttt ccctgaactt tcaaagctgc attgataccg gcaatcagcc cttgtccagc
    11821 cgcttcttca taaccgcttg taccatttgt ttgtccagct gtataaagat tttcaacgac
    11881 tttagtttct aatgtcggac ggagctggtg tggctctact acgtcatatt cgatagcata
    11941 tcctgtacgc atcatttcta ctttttctaa tccagcaata gaatgaagca tgttgacttg
    12001 aacatcctct ggtaaagaag tagataaacc ttgaacatag acttcttccg tatttaagcc
    12061 ttctggttcc aaaaataatt gatgacgtgg tttgtctgca aaacgcacga tcttatcttc
    12121 gatggatgga caatagcgag cgcctacccc ttcaacgatc cctgtaaaca ttggggcacg
    12181 atgtaagttg tctcgaataa tttggtgagt cgtctcacct gtataggtca gccagcaaga
    12241 ttcctgatct agtttatagg cactatctgg tgtgctgaag ctaaaatgat tcggctcttt
    12301 gtcaccaggc tgctcttctg taacagaata gtcgatcgta cttgatttta ctcgaggcgg
    12361 tgttcctgtt ttgaaccgtt cgatagcaaa gccaagttct tttagatgat tcgctagtcc
    12421 aatagaaggt tgagagttat tcggacctga tgaatatttc aattcaccga taatgatttc
    12481 tccgcgaagt gctgttccgg ctgtaataac aactgctttg gcccggtagc tcgctcctgt
    12541 ggacgtcact actccatgac aaacaccatc ttcgacgatc agttcttcga cgattccttg
    12601 tcttagcgtc aaatttggtt ctttttcgat cgtatgtttc atttctgttg aataagcatg
    12661 tttgtctgct tgtgctcgca atgctcggac agctgggcct tttcctgtgt tcaacatacg
    12721 catttggata taggttttat cgatattctt acccatttct ccgcctaacg catcgatttc
    12781 tcgaacgaca acgcctttag ctggtccgcc tacagaagga ttacatggca tgaatgctac
    12841 catgtccaaa tttattgtca gtaataatgt ttttgatccc atacgtgcag cagcaagtgc
    12901 agcttctgaa ccggcgtgtc ctgcgccaac aacgatcacg tcatacgcat ctgcttcata
    12961 atgttccaat cgtttgttcc tccttttatt ttcctaaaca aaattgactg aataattgag
    13021 tgattagctc atcttgtaca ctgtcgccga caacttctcc tagatagtcc cagcaacgcg
    13081 tcatatctat ttgaacgaga tccacaggca ttcctgaatc aatacctgca atgacttctt
    13141 caagtgagag ggaagctttt tctaacaacg cgatatggcg ggtattagaa acataagtag
    13201 cgtctctttc tcctgtttct ccgctgaaga ataaatcaga aatagctgat tccaaacgat
    13261 ctagtccgtc gtttttagct acggaaattg aaaaaactgg ttcattttcg atcaatttct
    13321 tcaatttttc ttgttccagc tgtgctggca aatctgtttt atttagaaga atgatccgtt
    13381 tcaatccact tgtcgcttcc aataattgtt catcttctgc tgtcagtggt tcgctttggt
    13441 ttagaacaag taagatcaag tctgcttctg ctaatgcttt tcggcttctt tcgaccccga
    13501 tccgttccac cacatcttct gtttcccgaa ttccagctgt atcgattagc ttgagcggta
    13561 ccccccgaac attgacatac tcttcgatta catctcgagt cgttcctgca atatccgtaa
    13621 cgattgcttt ttcttctctt aacaaatggt ttaacaaact tgatttgccg acatttggcc
    13681 ggccgatgat tgccgtactt aacccttccc gcagaacctt cccttgcttg gatgtagcaa
    13741 gtagcgcttg gattctttgt tggatcatct gcgctttttc aagcaataac ttcgtagtca
    13801 attcttcgac atcatcatat tctggataat caatatttac ttctacttgt gctaatgtct
    13861 ctaaaatctc ttgtctcaat gagcggatca aagcagacag attgccgtct aactgattaa
    13921 gagctagtcc cattgctttg tctgtttttg cccgaatcaa atccatcaca gcttctgcct
    13981 gtgaaaggtc aacacgacca ttcaagaatg ctcgttttgt gaattcgcct ggctcagcta
    14041 atcttgcacc ttctcttaac aatagctgca aaatttgatt gactacaacg atcccaccgt
    14101 gacaattgat ttctactaca tcttcccgcg taaatgtctt tggtgctctc atcacggaga
    14161 ccatgacttc atcgacaagc tgattgctct tgggatcaac gatatgtcca taatggatcg
    14221 tatgacttgg tacttctgat aatcgtttgt ttccagattg ataaacttta tctgctagtt
    14281 gcacagcttg atcgccactt aaacgaacga tactgattgc accttctcca ggtggggtag
    14341 aaattgccgc aatcgtatca aattctaaag taatttcagc cattttttca cttccttttt
    14401 cattttgcgc acaaaaaaag tgcccactcc accctttatg cacaataaac ggtgaacttc
    14461 gcactttgac tgcttgttct tgattaaatt ctggtcatag attatcacac ttgctataag
    14521 gaatcaagtg tttaaaacaa ttagatgaag ttgagcctgt tctgcttcca ttaccttata
    14581 tttaattgtc cttggatgtc tctttgcgtt ctttggttta ttcgaagaga agatactgta
    14641 taaacaaggc tttatctttc ttaaaaaaaa cggtacacta aatggtagcg ctatctaaac
    14701 tatacacttt tgaaaggaga aaatatgtga gcccgttcag atctgctgca ttggaagaag
    14761 aaagtgaccc tcggatcaac gatgtcgttt tatctacagg gaatatcaac cagaaatata
    14821 tcgtccgcga tatcgttttt gttgtggaat gcttagaagc tgatttgttt gacccatctt
    14881 ttcccaaaga tgaccttttc ttaacaacga agaggaaatt aaagacgaag gtacttgaat
    14941 atggtgcgaa tgcagtaatc aactgtcatt ttgagtatca gcaaaaaaaa cgcgacggaa
    15001 agtattttct ggaacttttt gcttatggaa cagtgatcca atttatccaa acaacgattg
    15061 gttaaatgaa acagacaaaa aaagcggatc aaaactgaag acagtttcga tccgcttttt
    15121 tctatcaaag ttagtgacta cttcatcgta tactctgata agaatgattt ctagtaaaat
    15181 atacttggtt tgaaaaactt tgtcaacgtg atttattcaa aaagtaatgg aatcaataat
    15241 attttttagc aggttctact actaaataac gataaggttc ctcaccttcc gaatgtgttt
    15301 ttacatgttc atctttgctt aatgcagcat ggatttgttt acgttcaaat gcaggcattg
    15361 gttctaagaa aacaggctgc cctgtccgtt ttactttttc agccgtgcga tcagctaaac
    15421 gctgaatgat ctcttggcgc ttttcacggt aattgcctac attgactact acagacagct
    15481 tatttcttgc taaacgatgg atgaagactt gtgccagata ttgcaatgca ttcagtgttt
    15541 ttccatgttt cccaatcagt atgccttgct tttgtgtttc taaatggaaa acaaccatat
    15601 tatttgattc tctttccatt ttgacaagag ctggagcatc aagctgtttt gaaatttcag
    15661 taagataaac tgccaattcc gttaaagctg cttcatcacc tagctcactt acttcttctg
    15721 ttttttcttc aagaatcgtt tcgttgacta cctcgatctc agctgttggt tcttcttcca
    15781 aaatttcttc tacagtctgt tctactgttt cagaaacttt ttcgctgata cttggctgga
    15841 tcgaaagccg tgcattcttt cgacccattc ctaggaaacc tttttttcct tcttccagta
    15901 tttcgatttc gacttggtct ttggccaaac ccaaatcagc cagacctagt tggacagctt
    15961 cgtctattgt tgcagcttca taaatcggca ttagcattac ctcccctatt atttccgttt
    16021 tttcttagga ttcattgctt tcttaagagc acgttctctt tcgcggattc tccgtgcttc
    16081 ttcttcacgc tcttttcgaa ttttgaaagg gttgttgatc agcagcgttt gtacaacttg
    16141 gaaggcattg gaaactaccc agtataatga aagaccgctg gctaaattga cacccatcac
    16201 taaaatcatt acaggcatca cgaaattcat gatcttcatt gatgcattcg attcaatttg
    16261 actcatgcta gataaatacg tacttgcaaa agtaaagatt gctgctaaaa tcggcaaaat
    16321 aaatgttgga tccgcgttcc ctaagttcaa ccataagaaa tgcccttgtt tcaactctgg
    16381 tacacgtgaa atagattgcc ataacgccat caagattggc atttgtacta ataaaggaag
    16441 acaaccagca tatggattga cgccattttc tgcatataaa cgctgctgct cttctcttaa
    16501 cttactttgt gtttccggat ctcttgaact atattgttgc tgcagtgctt ttaatttcgg
    16561 ttgtaattct tgcgttttac gcatgctctt tgtttggaaa tgcatcaacg gcaataggat
    16621 cacgcggatg atcagtgtaa ataaaatgat ccctatccct gcattaccaa atgacaacgc
    16681 cttgatcgcc tcagcaaagt aataaacaat ataacgatcc caaattcctg tactctgtgc
    16741 gctgacttcc ccagttccgc aaccggataa gacgatgacc aaactaacga taccggccat
    16801 caaaagtaaa cgtttgtatt tcttcactta ctcttttcct ctctgtcgat tatttttgca
    16861 agttttaaga cgtggactaa attggagcga acctcatcat aagtcaaccc cttcacactt
    16921 ggtctagcaa tcacaataaa atccaactcc ggatcaattt cttcttttat agaataaact
    16981 gctgctcgga tttttcgttt gacttcattg cggtttaccg cgttgccgac cttttttcct
    17041 acagataaac caactcgaaa atgaggctgt cccggttttt ccaaacggta aacgacgaat
    17101 ttgcggttag caaaagaatc tcccgtatgg aacacggctt gaaactctcg ttcttttttt
    17161 actcgatacg cttttctcat tagttttctt tccttttcta tctctcactt acgctcataa
    17221 tcataccata attacgattg tcctttcatc atttccctaa aaaagagtat aaaaaaaacc
    17281 actgaaaccc cagtggccta agctgaaaga acttttcttc ctttgcgacg acggctagct
    17341 aacacacgac gtccattttt agtactcata cgtttacgga atccgtgaac tttttgacgt
    17401 ttacgtttat ttggttggta tgttcttttc atatattcca cctccaaaat gttcttatct
    17461 attttataaa cacagacata ctttgatagt ataacgacaa agtagtgatt ttgtcaatat
    17521 aaaatagaag atcgattgtt aaaacttatc cacaagatta tttttttcta tgggaatcct
    17581 atgtggaaaa agttatgcac agggattatc cacagataat aaaaggctgg ttttacacag
    17641 aaaacacacg tgtaaaaaaa gtaatccaca atcaggcgat ttcctgtgta ttccgtctcc
    17701 taactattgg tatatctgta ttttttatcc acagacgcaa gaggaaatct attggttttt
    17761 acgagttttc cacctgtgca ttagtggtgt ggaaaacttt ttttgacaac tgtgtttttc
    17821 tcttttttct ttttgtcgat tgtgtggata attgtaatta aaaaattttt ttcttcggtt
    17881 tctgtggaaa actattgaaa aaaatgctaa attagtacta gttattcact ttttaatgaa
    17941 ggaggaggcc ttatatggta tccctcgatg ctttatggaa tgaattaaaa gcaacatacc
    18001 aaaaggattt atcgcctgct agctataata catggatcga aacagcgaac cctcgaaccc
    18061 ttgatcaaaa ccagctcgtt gttgaggttc ctagcaagat tcataaagaa tattgggaaa
    18121 aaaacctggc gacaaaaatc gttgaagtcg gttatatgtt atcaggaaat gagattatcc
    18181 ctcgctttat tactggcgaa gaagcagagc aagaagaagt tatagaagaa aaaaatccaa
    18241 aagttgtggc acctagtccg ctaaaaaaag ccatgctgaa ccctaaatat acctttgata
    18301 cgtttgtcat tggtaaagga aaccagatgg ctcatgcagc agcgctcgtt gtagcagaag
    18361 atccaggttc tatttataac ccgctcttct tttatggagg cgtgggactt ggtaagactc
    18421 acttgatgca tgcaattggt catcaaatgc tgcaaagtca gcctaatgcc aaagtaaaat
    18481 atgtaagcag tgaaactttt gctaatgatt tcatcaattc gatccaaaac aaaacagcag
    18541 aagagttccg tcaagaatat cgaaatgttg atttactgct agtggatgac attcaatttt
    18601 ttgcagaaaa agaagctacg caagaagagt ttttccatac tttcaatgcc ttgtacaatg
    18661 aagggaaaca aatcgtacta accagtgatc ggttgccaaa cgagattccg aaactgcaag
    18721 aacgccttgt ttctcgcttt gcatgggggc tgtctgttga tattacaccg ccggatcttg
    18781 aaacaagaac agcgatttta cgtaaaaaag cggcggctga acgtctagag atcccagatg
    18841 acacactaag ctacatcgca ggacaaattg attctaacat tcgtgaatta gaaggtgcgc
    18901 ttgttcgagt gcaagctttt gctgcgatga atagtgaaga tatttcgact agtctagctg
    18961 cagacgcact gaaaacgtta aaatcaggaa aaggacatcc tcaattgtca atcctgcaga
    19021 tccaagaaga agtggcaaag tattatcaca tccaattgaa agatctcaaa ggaaagaaac
    19081 gcgtcaaatc tattgttgtt cctagacaaa tcgctatgta tttggcacgt gagctcacag
    19141 ataattcttt accgaaaatc ggagctgaat tcggtggcaa ggatcataca acggttatcc
    19201 atgctcatga aaaaatccag cagctgatgg attctagtgt cagtatgcaa aatgaagtat
    19261 cagaaatcaa aaacttttta ctaaattaat ttgtcgattc tatcaactga aaatagcatg
    19321 tggataaaag aaaaaaacgt tcaaaagtta tacacacttt ttccacagct gttttttcgt
    19381 ttctctctta cgttttttga g
//
LOCUS       DAZULY010000022        19393 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000022 DAZULY010000000
VERSION     DAZULY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.022"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(317..1342)
                     /locus_tag="ACG81A_000567"
     CDS             complement(317..1342)
                     /locus_tag="ACG81A_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5013961.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1617..3302
                     /locus_tag="ACG81A_000568"
     CDS             1617..3302
                     /locus_tag="ACG81A_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFS5013962.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3316..4599
                     /locus_tag="ACG81A_000569"
     CDS             3316..4599
                     /locus_tag="ACG81A_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5013963.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4669..5538
                     /locus_tag="ACG81A_000570"
     CDS             4669..5538
                     /locus_tag="ACG81A_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5013964.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5535..6371
                     /locus_tag="ACG81A_000571"
     CDS             5535..6371
                     /locus_tag="ACG81A_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5013965.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6397..7464
                     /locus_tag="ACG81A_000572"
     CDS             6397..7464
                     /locus_tag="ACG81A_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFS5013966.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7461..8288
                     /locus_tag="ACG81A_000573"
     CDS             7461..8288
                     /locus_tag="ACG81A_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS5013967.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8333..9358
                     /locus_tag="ACG81A_000574"
     CDS             8333..9358
                     /locus_tag="ACG81A_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5013968.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9355..11538
                     /locus_tag="ACG81A_000575"
     CDS             9355..11538
                     /locus_tag="ACG81A_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFS5013969.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11538..12668
                     /locus_tag="ACG81A_000576"
     CDS             11538..12668
                     /locus_tag="ACG81A_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS5013970.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12665..13342
                     /gene="pgmB"
                     /locus_tag="ACG81A_000577"
     CDS             12665..13342
                     /gene="pgmB"
                     /locus_tag="ACG81A_000577"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS5013971.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13426..13953
                     /gene="msrA"
                     /locus_tag="ACG81A_000578"
     CDS             13426..13953
                     /gene="msrA"
                     /locus_tag="ACG81A_000578"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS5013972.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14154..14840)
                     /locus_tag="ACG81A_000579"
     CDS             complement(14154..14840)
                     /locus_tag="ACG81A_000579"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFS5013973.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            14920..16458
                     /locus_tag="ACG81A_000580"
     CDS             14920..16458
                     /locus_tag="ACG81A_000580"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFS5013974.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16578..17045
                     /locus_tag="ACG81A_000581"
     CDS             16578..17045
                     /locus_tag="ACG81A_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFS5013975.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17579..18007
                     /gene="rplM"
                     /locus_tag="ACG81A_000582"
     CDS             17579..18007
                     /gene="rplM"
                     /locus_tag="ACG81A_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFS5013976.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            18023..18415
                     /gene="rpsI"
                     /locus_tag="ACG81A_000583"
     CDS             18023..18415
                     /gene="rpsI"
                     /locus_tag="ACG81A_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS5013977.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18503..>19393)
                     /locus_tag="ACG81A_000584"
     CDS             complement(18503..>19393)
                     /locus_tag="ACG81A_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5013978.1"
                     /translation="LPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMF
                     DIAEILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFN
                     NHLLTFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGR
                     KDTVIQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYL
                     NYRINSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNT
                     PDIVDKAVFEAFQRGLNKCD"
BASE COUNT         6265 a         3512 c         4116 g         5500 t
ORIGIN      
        1 cagtagacat gtctgttatt ctatttatgt tttagagata agtatcccca aataacatgg
       61 attgaaataa gggtaaatgt gtttaaaaaa acgaactata aaaacttata cacaacgtgg
      121 ttaaaaatag ttttttcttc cctgtggaaa aaatagttta acacaaacat tatagttatc
      181 cacagctatc taaaacagat ggaaaataag aaaaattcgc taaaataccg ggagggggta
      241 acgagggatg agtgattgaa aaaagaacag agtaactgac ttatcctcag tgtttctgtt
      301 cttttataat tctgttctac atttcttgtt gttggatcga atctgtgaga gtaatctcgg
      361 ttgaaagaat gattcttttt tcttcagggt attggtggtg gattagttgg aataaaagtt
      421 ctgtggcttt gattcctaac tgatttgtcg gaatgtctat ccgagtcaac tgtggcgaaa
      481 atagggagga caataaggga tcactgaatc ctgcgatcga aagatcatca ggtatctgct
      541 ttccagattg ttctgccact ttgtaaatac tcaatgattt taatgcatcg gtagaaaaaa
      601 tcgcagttgg tctttctcgt agcttcatta aatcattcat tgctgccatg ctatcttcta
      661 agcgaaagcc gccgtctata atccattcat tacggatatc taggtggtga tcaaataagc
      721 attgacggta accattgaga cgatcattgg atacataaac ttctgtcgga gcatggagac
      781 agccgatatg acggtgtcca ttcttgatca gtgtttccac caaatcgtaa ctatcctgga
      841 aattatccgt gtccactgag tagatgttcg tgtagtcgtt tccgactttt ccgattacca
      901 caataggaat attatattga tccaattcaa aaagaagtgt ttcatccatc gtagagctta
      961 aaataataat tcctcggatc attttttctt ggatcttttt cgcgcatttt tctagctctt
     1021 cttctggtgt ttttgccgtt tgtagaatca agtcgaagtt attttgttca gaaacggcag
     1081 aaatggattg aataacatct gaaaaaaaag gattgccata ggtcgtatga gaagtacgat
     1141 gagataatgc tagaatcgcg tcaaaaccat ttcctgacag tcctcttgca attttgctcg
     1201 gttcataatt caattctttg attgcttgaa gtacacttgc ttttgcttta tcagagatat
     1261 ttggctggtt atttaatact ctggagacag ttgattttga gacattagct tttttcgcaa
     1321 tgtcataaat agtaggtttc aattttatat gcacctcctt tttgggagcg ttttagaata
     1381 attccatagt acaatagaca ttcttttttg tctatgattt tgaaaattta ggaatagctt
     1441 gcatgatcat gagagaccat atttttttaa gaagctatta attattgata cataagcttt
     1501 cttatttgta tttttgtgta tatcaaaatt atttaaaaaa atgctattga cagtaagcgt
     1561 tttccataat atgatcaaat cgttgggaac gctcccaaaa aagaaagggg aatccgatgt
     1621 atacaaaact tttacaagcg atttatcgcg actcagattt gtctgagaaa accgcctcta
     1681 tgattgaaca aaaagtcagt gtttatcagc agaagaaagg ccaggcaggc aaactgcatt
     1741 gggacgaaga agatattttc ttgattactt atggggatca attttttgaa gagcagcaac
     1801 caactttgcg tacatttaag aaattttatc aaaagtattt ggcagataca tttagagttg
     1861 tacatttctt gccatttttc ccatatacat ctgatgatgg tttctccgtt gtaaattatc
     1921 aggcaatcaa cgaccgtttg ggcacatggg aagatataga agaaatgaga agcgaaacac
     1981 ggttaatgtt cgactttgtc tgcaatcata tgtctgccga aagtacttgg tttcaggaat
     2041 atttaaactt gaatgatacg tacaaagatt tttttattga tttgccactg gatacagata
     2101 caagcagcgt gactagacca aggaccagcc cgttacttac aacgttcatt gacaaaaacg
     2161 ggaaagaaaa acacgtatgg acgactttta gcgaagatca agtagacttg aattttaaaa
     2221 atccagaagt tttattgaaa atgatcgata ttcttttgtt ttacttagaa aaaggcgctt
     2281 catttattcg cttggacgct gtgggatttt tatggaaaga actaggaact tcttgtatac
     2341 acctgccaga aacgcataaa atcatccaat tattccgtgc aatcgctgat gaagcagctc
     2401 caggaacggt attgatcact gagacaaatg tgccacacaa agataatatc agttattttg
     2461 ggaatggcga gaatgaagca cagatggtct atcagttttc gctaccacct ttgacccttc
     2521 aagcgattcg ttcgggtaat acaagtaagt tgaaagaatg gttaaaaacc atcgatttcg
     2581 catcgaaaaa aacaactttt tttaattttt tagcctccca tgacggtatc ggattgaatc
     2641 cggtacgagg tattctgccg gaaaaaacga tccttgaatt agtggaacag ctgaagaaag
     2701 agggtgcccg agtaaactac aagaaaaacc cagatggaac gaccagtcca tatgagataa
     2761 acgctacgta tctagatgcg ttaagttctc caacagatac gacggaatgg agaagcaaac
     2821 gctttttagc tgctcattca tttttggtaa cgttaccagg cgtgccggca atttatattc
     2881 aaagtgtgct gggaagtcga aatgattaca aaggcgtaga aaaaacagga gagaatcgaa
     2941 aaatcaatcg gcaaaaattt ttgttagcag atattgagaa agagttgcag acagatccat
     3001 taagaacagc agtatatggt gaattgaccc agatgctatc tattcgaaaa aaagaaaaag
     3061 ccttccatcc gaatagtttg atttacttac tggactcagc agattctttt atcagtttta
     3121 ttcgcgaagc gaaggaagag aaagtcttag ttatccataa tttttccaat caatcagcag
     3181 gttatctttt accaaaagga gattacctag acctgttttc tggacaaatt atccattgca
     3241 gtcaagaacg aaagttgata ttccagccgt atgaatatat gtggctgaaa ataataaaat
     3301 aagaggtgga aaaaaatgaa aaaaattatt gttactacct gtctgctagc tactggattt
     3361 cttcttactg cttgtggtga aagtcaaagc tctaacgaat cacaacagac aacgattcaa
     3421 ttcatgcact cttctgtcga acaggaacga ttaagtgtga tcaatgaact ggttgctgat
     3481 tttgaaaaag aaaatccaga catcaaaatc aagcaagtgc cggtagaaga atcagcgttc
     3541 aatacaaaaa tcgttacgtt agctcgttcc ggtaaattac cagaagtaat ggaagtcagt
     3601 caggattttg ctaaagtgat ggataaagat gaactgattg atcagacggc agtccaaaac
     3661 gttatcgagg aagctggaga agataattat tatgacggag ccaaaaacct tgttcgttca
     3721 gaagatggaa aaagctatat tgctgcacca attagcggct gggttcaagg tatttggtat
     3781 gacaaagaaa aattatcaga agctggtttt tcagaaccag aaaattggga tgatatttta
     3841 aaaattgctc aacatttcac agataaagag aataaacaat atggaattgc tatgcctact
     3901 gctgaaggaa caatgagtga gcaagcgttc tctcaatttg ctttgtcaaa tggagcaaat
     3961 gttttggatg atgaaggcga ggtaacgatc gatacggaaa agatgagaga agcattgtcg
     4021 ttttatcaaa acttatccca atacacgatg ccaggctcta atgatgtgac agaaatcaaa
     4081 gatgctttca tgaatggaac agcaccaatg gcaatctatt caacttacat tttgccttct
     4141 gtttatgaag cggggaactc agaaaatatc ggttttgcta tcccaacaca aaaagatcaa
     4201 gctgtctatg gcacagtatc tggattgacg atttcttctg gtctagacga gaaacaaaaa
     4261 gaggctgctg aagcattcac agctttctta tctcaaccga aaaacatgga aaagtgggtc
     4321 ttgatgtctc ctggtggtgc tcaaccagtg aataaacaag tagtcgaact ggatggttac
     4381 aaagaaaatg aagtcatcaa gtcttttggc gagctgccat ctgagattgc tagtgctttt
     4441 gatgaagtac aagtctttgg actagtcggg gaaaagaatt tcacgaaaat gggagatatc
     4501 acaagctcag gtgtgatcgg aaaagcagtg aaccaagtaa ctgtcggaaa cgaagatgtc
     4561 gatcaagctt tagctgacgc acaaaaaaat acgaaataac aagaagttct gccaatggtg
     4621 ttcgaaaaaa gtagatagct attggcagat tatcaggggg aggtttcaat gaatcgtcca
     4681 aggaaacatt ctgacttaaa attagccatg ggacttttag caccaagcat gcttttatta
     4741 gctgttttag tgctgattcc aatgctttcc aatattcaga tcagtttttt cgatatgccg
     4801 ctgaatccta actcagacgc cgtttttatc ggattaagca attatcttga aattttaagt
     4861 gatagtgaat tttatcgttc tttaggttta acagttgctt acacattctt agttgtagca
     4921 ggcagtacag ctttaggatt agccgtagct attttgctta accgcaagtt cagatttagg
     4981 aaaacggtgc gttcactgat tttattaagt tatgttacgc catctatttc tttgattttt
     5041 gcttggaaat atatgttcaa taatagttat ggaatcgtca atgatctgtt agtagatagg
     5101 ttgcagcttt tcaacgaagc accgctgtgg ttcgaccagc cagttgctag cttctttatg
     5161 gtcgttttgt ttgctatctg gagatactat ccgtatgcgt ttatttcatt tttggctatt
     5221 ttgcaaacag tcgacaaaac gctttatgaa gcagctgaaa tggacggtgc aaatgcttgg
     5281 cagaagttca agatcgtgac actgccggca atcaaaccag tactagcaac tgtgattgtt
     5341 ttacgatcta tctggatgtt ctatatgttt acagatgtct atctactgac gaataaagtc
     5401 aatatcttag gcgtttattt gtatcagaca gcctttgcgt tcaatgatct tggaaaagca
     5461 gcagctatat cgattctatt gttcatcatt attgcagcgg tcgtcttgct ctcacgaaag
     5521 aaggtggact tgaaatgaaa aataaaaaga gcaggaaaat cttattttac gcgacgattt
     5581 tgttgctatt agctgtcaat ttgtttccat ttttgatcat gctgatgaca tcattcaagg
     5641 attcacaaga agcaattgca acgacacaaa ctatttttcc gaaagaatgg acttttgaac
     5701 attatcagga tattttcaat gctgagattt ttccattcat catgtatttc aaaaatagtt
     5761 tagtgatctc cttgatagcc tccgtaactg cagtagtgat tgggatcagc ggggcatacg
     5821 ctttgtcaaa actacgcttc aaagctcgct cgaaaatcaa tgctagcttt tacaccgttt
     5881 atatgttttc cggtattttg atcgttgttc cattatttaa aatcatttcc ggtctaggtt
     5941 tgtatgatac caatacagca ctgattatca cgatggtcgt tcaaacactt cctactgcga
     6001 tattcatgct aaaaagttat tttgacacga ttccgactga tattgaagaa gcagcaatga
     6061 tcgatggtct tagccggatc cagatcatta ttcggatcat tataccattg gctatttctg
     6121 gaatcatttc tgtcttcgtt tattgcttta tggtttcgtg gaatgattat ttatttgctt
     6181 cgattttcct gtcgacctcc gaaaaattta ctttaccaat cggtttgaac acattattca
     6241 gtacaccaga ctacatttgg ggacgaatga tggccgcttc attggtgacc gctttaccag
     6301 tcatcattat gtatgcgtta tctgaacgat ttattaaagg tgatttagtc gaaggcggcg
     6361 taaaaggata aaaagaaaag aaagaggaaa gaaaagatga aaaaattagt tgcattagaa
     6421 ccaagaatag ccggattcaa agattacgaa gaccgtgaga tcgaaaagaa cgaagtacgt
     6481 atccgagtaa ttttcgctac tccaaaacca ggaacagaaa ttgtcgactt tagaggatta
     6541 agtccattta tcgatgaaga attttcagaa gaatggcgcc tattcttgcc aagagaagaa
     6601 ggaagccaac gcggtgtggt atttggcgaa tttcaattag gaaatatgat tgtcggtacg
     6661 atcgaagaaa cgggcagttt ggtgacagag tatcaaaaag gagagttagt ctgcacttat
     6721 ggacccattc gtgaaacgct gattacaaac ggtgttgata attataaact acgtaaactt
     6781 gatcggttgg atcaatggaa aaatgcagta tgttacgatc ctgctcaatt cgctttaggt
     6841 ggtatccggg aagcacatgt tcgagcaggt gatgctgtag caatcttcgg tttaggcgct
     6901 atcgggcaaa ttgcgattca attagctaaa aaagcaggtg ctactaccat cgttgggatc
     6961 gatcccatag cgcagcgtag agaattagca gaaaaacacg gtgccgatta cacgctagat
     7021 cctatgaaag cagatgtcgg ttttcgtcta aaggaattga cagataaacg tggagtagat
     7081 tcagttattg aaacgagtgg cagtgctgcc gcattacaag cttctttaat agggatcggc
     7141 tatggcggga tcatttctta tgttgccttt gctaaacctt ttccagctgg gtttaatctt
     7201 ggtcgcgaag cacatttcaa taatgcaaaa atcgtctttg ctcgagcatc tagtgaacca
     7261 aacccagatt acccaagatg gaaccgtaaa cgaatcgagg acacatgttg ggaattattg
     7321 atgaacggtt acttggattg tgaagaaatc attgatccag ttgtgccatt tgcagaaagc
     7381 gcagaagctt acatgcgtta tgtagaccag cacccagaag aaagtatcaa aatgggcgtt
     7441 caagtccgag gaggagaaca atgaaattcg gtacgcaaga tcaagcattt ttcccaagag
     7501 acattttaga aaaatttaga tttatcaaag actgtggatt tgaagaatac gaaatcgacg
     7561 gtaagttgtt aatagaaaat atcgacacag tcaaacaggc aatcaaagaa acagggatcc
     7621 cagtagtgac agcttgtaac ggctacgatg ggtggatcgg tgactttatc gaagaacggc
     7681 gtcttaatgg tgtacgtcaa attgctgaga ttttgcgtgc actaagcgag gttggaggca
     7741 aaggaatcgt tatcccagca gcttggggaa tgttcactta tcgattaccg ccgatggttt
     7801 caccccgaag caaagaagga gactataaag cagtcagcga atcacttcgt tatttagata
     7861 aagtagccga agaaacaggt acggttattt atttggagcc tttgaaccga taccaagacc
     7921 atatgatcaa tttgttagcg gatgcgcgtc attacattga ctctaatcaa ttgaaacatg
     7981 tgcagatcat cgctgatttt tatcatatga atattgaaga agatgatatt tcaaaagcat
     8041 tgactacgca aaaagagtat gtgggtcata tccatttagc agataatcat cgctaccaac
     8101 caggatctgg cgcaattgat tttactcgcc attttcaaac gctacttcaa aatgagtatg
     8161 aaggttatat ggtatatgaa tgtcgtttgc gaggagaaaa tgaagtgaaa atttatcagg
     8221 aatcattgca atacttgaaa aactgtctgg aaacagcgcg tcatttaaat gatttagaga
     8281 aagaatagag aaagaataac aataagtaaa taagcaaagg agagagaaat aaatgaagaa
     8341 agtagcaatc ataggcgctg gacaagtagc agaaaaagta catgctgctt attatcaaaa
     8401 aagatcagat gtcttagaat tggccgctgt ggttgatcca gtcagccagc gagcagaaga
     8461 cttttgccga cgtacaggat tttctcatgc gtatacatca gttacacaaa tgctgaatga
     8521 gatttctccc gatatcgtga gtgtctgtac acccaatcgc tttcacttcg aacatgtatt
     8581 ggctgccctc gaagctaatg cttcagtgat gtgcgagaaa ccaccagcta tgacagctga
     8641 tcaagcaaga aagatggcag agacagccga taaaaacggg cagattttgg cttatggatt
     8701 ccagcatcgt ttttctgacg aagcaacaat gatcaagaat catcttgatg ttctcgggga
     8761 tatttattac gtagaagcga atgcgcttcg tcggtctggt gttccaggct ggggaacgtt
     8821 tatcgacaaa gaacttcaag gaggcgggcc tttgatcgat tatggcattc acatgcttga
     8881 tacagcgatg tatattactg gatttcctaa agtaaaaagc gttcgggcgt acagcttcca
     8941 aaaaattggt acgaaaaaaa attcagggaa attcgggaaa tgggatccaa gcaaattcac
     9001 agtagaagat gcaatttttg gcacactgga attagaaaac ggcaagatca tccaactgaa
     9061 tacttcgttt gctttgaata tcagagaaga caaactgaat gtggcttttt gtggcgatca
     9121 ggcaggtgct aatctgtatc caggcgaact attcgaagat gtagatggaa agctggtttc
     9181 cttacaaaag accacgccag ttaatgaccc acatgaacta agtatggctc attttgtgaa
     9241 tcgggcgctc ggtgaccaga ctcagatgat tgcagatggc tggcaaggat atgtcatcca
     9301 gaatctggtg gaaaatttat atcgttcggc agaaacagga gaagttatta ccttatgaaa
     9361 gtagaagaaa aacagtttga tttagagtat atcaataagt atgcgagtca gatgaccatt
     9421 ggaaatggat tttttggtat cagaggaagc caggaggaag actatcggaa tcaagtaagg
     9481 ggaatgtttg cagctggtgt atataaccga cctttaggag cagaatcagc cgagttagtg
     9541 aatctcccag atgtcatcag atgggaaata aaaatcgacg gtgaacattt ttctcttgag
     9601 acgatgaaag ttcttgcata cgagcgggca ctagatttag aaatgggaga gcttaaacgc
     9661 tatgcgctca ttgaaactgc tcaaggaaaa aaagtggaag tcattacttg tcgtgttgct
     9721 gatcaaaata atctccatca ggcagccctg aaagtggtca tcaagccgtt gaccgaagac
     9781 tgccaagtag atgtgaaaac gggtatcgat ggacagcaaa ccaattttgg tacacagcag
     9841 ttggttgaaa aagaactgcg agtatttggt gacgatttga tcagtgcatc ctaccagaca
     9901 acagaaagta aaattgatat tgggattgca gctaaattca ataaagcagg tgtattccaa
     9961 gcaaaaaatc gccggatcac cgcttccttt tcagaaaaga tcagaaaaaa tacgtccttt
    10021 acgctggaga agatcatcgt gctctctacc tctcttcagc aaacagaccc gtttaagaaa
    10081 agtattgccc aagcccagaa aagcgttagt tatacagaaa ttttagagca gtcgatgtct
    10141 tattggacag atttttggca tcagcatcgg ataacgatcc aaggcgattc ttctttcgat
    10201 cagttagcga tggacttcgc tgcttaccat ttacagatta tgactccccg caatgatcca
    10261 agtgttagtg taggggcgaa gggactaact ggcgaaggtt ataaagggca tgttttctgg
    10321 gatacggaaa tttttatcct gcctttcttt ttatatcagt ttcctgaagt agcaaagaat
    10381 ttgctgctgt accgttatga tcgattagaa ggcgcacggc aaaaagcaag agattatcac
    10441 tatcaaggtg cacttttccc atgggaaagc gcacacactg gtcaggaaga gacacccaaa
    10501 tttgctgcat tgaatatcaa aaccggaaca agacaagaag ttgcttcagc tgccgcagaa
    10561 catcatatca gtgcagacct tgcttatgcc gttcaagagt atgtccaagc aacagaggat
    10621 caatcgtttt tagaaacaaa gggaaaaacg ctcattcatg aaacagcagc tttttggctg
    10681 agcagagctg taaaagtcga tgatcattta gagatacaca gagtcattgg tccagatgaa
    10741 tacacagaat atatcgacaa taatgcatat acgaactatc tggcgcatta taatgtggcc
    10801 ttagctgtta aatatcaagc tggcgatccg ctttttttga aagaatgtca aaatttttta
    10861 gataagttat atttgccagt gcctaacaaa gaacagctga ttccgcaaga tgacacgtat
    10921 ctcagtaagc cagttattga tttgaccact tacaaagaac gtcctggcag ccagtctatt
    10981 ttacttgatt atgcccgtag cgaagtaaat gaactccaag tcagcaagca agcagatctt
    11041 gtcatgctgt tatacttatt acctgagtat ttttctcaac caatcgtttc taagaatttg
    11101 cattactatg aaaacaaaac aatccacgac tcatcgttga gtaaagcgat tcacgccata
    11161 gaggcttgcc gtatccaaga aatggaatgg gcgtatcatt tattccaaga agcgtgcctg
    11221 attgatttag gaccagatcc gcattcatca gatgacggaa ttcatgctgc ttcattagga
    11281 gcactatggc tagcagttat atttggtttt tcaggtatta cagtaacgaa tggaactctt
    11341 tctatttctc caagacttcc ttcagcatgg gaaaaaattt cttttccttt tatttggaaa
    11401 aatcacaggt tgctatttgt tttgacaaaa aaacaaatag taataaaaaa agagacgaaa
    11461 gaaacattac ctatttttgt caatggagac caataccagc tggaaaaaga actgatcttg
    11521 gaacggaggg gaaaataatg cgtattctga ttgcgccaga ttctttcaaa gaaagtctga
    11581 cagccttaga ggcagcacaa gccattcaat caggttttca agaagttttt gttcaggctg
    11641 aatatgatct gctacctata ggcgatggag gagaaggaac ggtttcatca ttagcagaca
    11701 gtttaaggtt gaagcggcaa acagcagtcg ttactaatgc atttggcaaa aacaaagaaa
    11761 tccagtacgc ttcagacggt caaactgcga tttttgaaat ggcagaaatc gtcggattga
    11821 aagatgtccc actaaaccaa caacagccac ttaggttatc cacgcgagga gtaggagaac
    11881 tgatacgaca attgttgcaa caaggtgttc gacacatcat catcggcgtt ggcggaagta
    11941 gtaccaacga tggcggcatc ggcatggcag aaggtctagg ttatgctttt tacgatcaag
    12001 ataatcaaaa agtagaagca ctgggagaaa atctaggtaa aattgctaca gttgataaaa
    12061 cgaacgtgcc tattgagctg attaaagcca aaatcactgt cattactgat gttactaatc
    12121 ttttatgcgg aaaagaaggc gcttcttttg ttttcggacc acaaaaaggc ttgaaagaaa
    12181 atgaattagc agtggctgac caagccatgc atgattttta tatgagattt gcccctcaag
    12241 taatcgaggc atcgggcagt agtgctggtg gtggaatggg tgcaggactg ttagcatttg
    12301 cttcagggaa aatagtctct ggcatcgact acgtattgga tttactggag ttcgataaac
    12361 gggtacagca agcagacctc gtaatcgtag gcgaaggacg tctagatgga caaagtctaa
    12421 aaggaaaagc gccagttggg attgctagaa gagtaccaga gggcgttcct gtgatagcta
    12481 tttgcggaag tgttggtaaa ggaagcgaaa aagcagcaga tgttggcatt tcggccgttt
    12541 ttccgatcct tcctgcagct gtgcctttac aagaagcttt acagcaagcc actgataatc
    12601 ttcatcgcac agctaaaaac atcgcatctt tacttcaagt cgttcatttt caaggaggta
    12661 atcgatgaaa atcaaagcag cactattcga tttagatggc gttcttgtag acacagctcg
    12721 ttatcattac gaagcgtggc tggttttagc taaccagctg tccattccat tcactgagaa
    12781 agaaaacgag caactgaaag gaatatccag aaccgagtca ctagagcgat tgctgtcatt
    12841 tggaaaaatg gaacagaaat tctcagaaaa agagaaatca gcctttgctg agcaaaaaaa
    12901 caatctatat cttcaagcga ttcaaaaaat ggatgaaaca tccgttttgc ctggtgcaat
    12961 agctgttctt gagtacttga aaaaaacaaa catcaagatc ggattgggat cagcaagcaa
    13021 aaatgcacgc ctcattttag aaaaaacgaa tttgacaagt tattttgatg tccttattga
    13081 cggcacgcaa gtatcaaaag caaaacctga tccagaagtt tttctcaaag gagcacagca
    13141 attgaatgta ccgccaaatg catgcttggt cattgaagat tcagaagctg gttgtcaagc
    13201 agctttggct ggtaacatgc atgtattggg aataggtgaa aacataaatc ttccttctgc
    13261 tgaatatgtg attcctgatt taaccgtttt tgatcaagta cgttcgtttt ggcatttatc
    13321 agaagccgta tcacgtatat aatagtttcc gttttttctg tcacaagcaa aacgttatcc
    13381 tccttttgga aatcaaggta tgatgagaaa aaagggggat tttatatgac aaaaaaagca
    13441 gttttcgcag gcggatgctt ttggtgtatg gtcaaaccat ttgaagagca gccagggatc
    13501 atttctgtga catccggtta tactggcggg catgtaccaa atccaactta tgaacaagta
    13561 acaacaggca caacagggca tacagaagca gtagagatcg aatatgatcc agaagttatt
    13621 tcttatgatc agttggtgga aatctattgg cagcaaaccg atccgactga cgcattagga
    13681 cagttcgctg atcgcggtga ttcttatcgt ccagtgatct actataatga tgaagaagaa
    13741 cgaaatattg cagagcgttc aaaacaagca ttgcaagaca gcgggcgatt cgataagcca
    13801 atcgtgacaa aaatcgaacc aagaagcgaa ttttatcctg cagaagatta ccatcaggat
    13861 tattacaaga aaaacaagct ccattacaat ctttatcgag aaggttctgg acgtgcaggt
    13921 ttccttcgtc gacattgggg cgtcaagaaa taagaagccg aaaaagctac ttatggaaca
    13981 ccgtatatca gaggttgtaa aaccagcatt ttgagctaat actgagggtc agctgattga
    14041 acaccattta gtggagaggt tgtgaacctg tcgcctagct ccaagtcaca aggatttggt
    14101 aagagaacac tatatatcag aggttgtgac aagtcgtttg tcacaacctt ttttcaatac
    14161 ctaagatgaa ggacaatcag caacgcactg ctcaaaaaag gaatgaaagg cagcttttcc
    14221 aacttttttt tatgacagat aaagtaaaca gaaaaaacgg atattccaaa cagactagca
    14281 agaaacaaga tctgaagcaa ctcttctaga gacaagccac cactccaaga aagcatcaac
    14341 agagtatcgc cagctccgag ataggattga ccatagcgaa ggcacacacc gcctacgata
    14401 aacgttaaca gcacactttg ccattggaag acgccggcct gcaaccaaaa aatccataaa
    14461 attgcccaag aacagaatag cgtgtgagaa tggaccatga agtaaaataa atccatcaac
    14521 gaaaatagca aagccgaaag catccatatg aacgtcgaaa aatttcttgc ggaagaaaaa
    14581 gtaaagaacc aggcaaaaag accaccacat aaggcttctg ccaacaaata gtagaagggc
    14641 aaccgacacc tgcaatagcg gcagcgaaat cgttggacga tgatcgagag aatcggaatc
    14701 aattcatacc aagaaagcga acgacagcat ttgacacaat gagatctagg ataaataata
    14761 gagtgaccta acggaatccg ctgggcggtt aaacataaaa atgatccgat acagcaacca
    14821 atgataaaat aaagtaacac gccatctccc ccttttcaaa accaagatac gcaagcttaa
    14881 actaaatttt tgtacataca taaaatttga ggtgaaaaaa tgaacgtttc aatcggatta
    14941 gatatcggaa ccacacaaac caaagcagtt gctttcacag atgaagccca agaagcagca
    15001 tccgcttatt ttcgctaccc tttgatccaa gaaacacaag ggatggcaga acaagatcag
    15061 gaattgatct ttcaaggtgt ctgcaccgtt ataaaagaag taattaaaca gttgccttca
    15121 gacacaatca ttcaatttgt ctccttctcc tctgcgatgc atggcctgat attgttggat
    15181 gaagagaaaa caccgttatc tcgaatgatc acttgggcag ataaccgagc agcggacgaa
    15241 gcagaaaaac tgaaatatac cgtgcttggc aaagaatttt atcggcagac tgggacgccg
    15301 attcatccaa tgacaccatt gaacaaaatc aagtggctgc aaaacagcca acctgaatta
    15361 ttcaaaaaaa ctgcttattt tgtcggtatc aaagactata tcttttatag attgtttggc
    15421 gaactgatca gcgactacgg aacagcttca ggaactggat attttgatat ccatgaattc
    15481 caatgggcag aagaagtgct tgactacttg aacattcgaa tagagcaatt gccgcaagtc
    15541 tttccttcca cctatcaagt aacaggtcta ccatcaaaaa cagcagcaga actaggtttg
    15601 ccaaatgata tatcatttgt tttgggaggg gcagacgggc cgctttctaa tctagggtta
    15661 ggtgcttttg gtgatacgat cgccgctttg accgtaggaa cgagcggagc acttcgtttt
    15721 attactactc agcctcagtt ccatccagaa atggagacgt tttgttatgt tctggatcag
    15781 aaacattggg tagtgggagg agcgactagc aatggtgctg gcatctttga ttgggcaggg
    15841 catacatttt tacaggaagt cgttcaatct gctaaagaaa aaggagaaaa tccatatgat
    15901 gcactgcttt cggctatcgc ttttgtgcca gcgggtgcca atggtctgat ttttcatccc
    15961 tatttacttg gtgagcgagc accgctttgg gacgcagatg cgtttggcag ttttatcggt
    16021 ttacagcggc aacatacaga aaaagaaatg atgaaggcga ttctagaagg gatctgtctc
    16081 aacctttatc ggattttaaa aggagtttgc caggaaaatc agctcacaga gatccgtgct
    16141 acaggcgggt ttgctcaatc tccgattttt cgtcagatga tggctgatgt actgggttac
    16201 ccgcttgttt tccctactgt cacagaagca tccgcattag gagctgtgat tttaggctgg
    16261 agaagcatcg gaaagatcca aagtatcagt gaagcacagg aattgataga attgaaagag
    16321 caagttggag tacaagaaac tgatcataaa aaatatcaga aaatctatcc attatttgtt
    16381 tcgacacaaa agcatttagc acagacttat cagcaatttg ctcaactgcg agaagaatta
    16441 gctgattctg aaaaatgatt ggctttacgg ataaaaaagc gaactgaaaa tcgttctcag
    16501 gtaaatgatt ttgttgtact tttcattgaa agtctactat tattttaagt gtaagaaaat
    16561 gttaggaggc aagaaagatg aaattccaac aaaccaaaga agtattaaat caattagttg
    16621 cagatctaag ccaaatgtct gttgttgttc accaaacaca ttggtacatg agaggaccag
    16681 gtttccttac attgcaccca atgatggatg agttcatgga tgatttgaac gctcagttag
    16741 atgaagtatc cgaacgcttg atcacattag acggttcacc ttattctaca ttgcgtgaat
    16801 ttgcagataa cacaaaaatc cctgatgaaa ttggccgctg ggatcgtact attccagaac
    16861 gtttggaaac acttgttgca ggctatcgtt atctagcaga tctttaccaa aaaggaatcg
    16921 aagtttctgg agaagaaggc gacgatccaa cacaagatat ctttatcgaa ttcaaaacag
    16981 ccacagaaaa acgtatctgg atgatccaag ctcatttggg caaagcgcca gagatcgacg
    17041 cgtaaaaact gttgttgaac ttgaccgaaa aagaggttgt gaaatcagcg ttttgacctg
    17101 agtattagag aaacaaacgt caaaatagtt cctcatattt tgccgttttt tgagctaata
    17161 ccgagggtca gctgattgaa caccgtttac caagaggttg taagcctgcc gttcagctcc
    17221 gagtcacaag aaacaatttt aaaaaacagc ttcttatgtt ttatggatat tgtgacttgt
    17281 gaccgaggag ttggcaggag aacaccgttt attcaaaata aggggttgtg acaagacttt
    17341 tgtcacagcc tctttttgtt tttcattttg acaggaaacg acccttttat ataaaagaaa
    17401 gaaaagaagt aaagtttcat ataaattttt caatcaagca gaaaaacgtt gacattgact
    17461 agattagcgg gtaatatgtt aaatggtatt gtttgcccca caaagagccc cggaaactcg
    17521 aagtgtatgt caaacatcag aacttaaatt ggaggaaaat aacgtgcgta caacatatat
    17581 ggccaaacca ggcgaagtag aacgtaaatg gtatgtagta gacgcaactg atgttccatt
    17641 gggacgtctt tcaacagttg ttgcatctgt actacgtggt aaaaataaac caactttcac
    17701 acctcatttg gatactggcg atttcgtcat cgtcatcaat gcagacaaag tgaaattaac
    17761 aggtaaaaaa gcgactgaca aaatttatta ccgtcacagc aactacccag gaggattgaa
    17821 atcaatcact gctggcgaat tgcgtgctaa aaattctcgt cgtttgatcg aaacttctgt
    17881 aaaaggtatg cttcctaaaa acactttagg ccgcaaacaa tttacaaaat tgaacgtata
    17941 cggtggagca gaacatccac atgcagcaca acaaccagaa gttttagaca tcacaaactt
    18001 aatctaatag gagggaattt cattggcaca agttcaatat atcggcacag gccgtcgtaa
    18061 aaacgcagtt gctcgcgtac gtctagtacc aggaactggc aaaattactg taaataaaaa
    18121 agatgttgaa gaatacattc cacatgctga cttgcgtgaa gtaatcaatc aacctttcgc
    18181 tgttactgaa acaaaaggcg catatgacgt atttgttaac gtaaacggcg gaggatacgc
    18241 tggacaatca ggagctatcc gtcatggtat cgctcgtgca ttgctagaag ttgatcctga
    18301 cttccgttta gctcttaaac gtgctggctt acttactcgt gacgcacgta tggtcgaacg
    18361 taaaaaacca ggtcttaaga aagcacgtaa agcttcacaa ttctcaaaac gttaatatcc
    18421 tttatatatc aacgtttctg gcacttttca attatttgga aagtgcttct tttgttttta
    18481 agaacacatt taagaacaca tatcaatcac acttattcaa tcctctctga aaagcttcaa
    18541 aaacagcctt atcaactata tctggtgtgt tcacatatat ctttgttgta gaaatatctg
    18601 agtgtgttaa agcatttgaa atttgattca tatctgctcc accttgtcta gctatagttg
    18661 cataagtatg acggagtttg tgtggagata aatgaactaa gtcaggatgc cttcttttaa
    18721 ttgaattgat tcgatagttt aggtaatcgg catgaacagg acaatttact tctccagatg
    18781 gtttagtata agtaaagaga tactgatcta acgtttgctt aatttttaat ttcaacaatt
    18841 gatcagcttg taccgatttc cattcactta gcaatgtcat tacaacttct ggtacttgaa
    18901 tgacggtatc ttttcgacct tttgtaaact tttttctttg atatttatct aaagtatgtt
    18961 taagacggac cgtttgtttt tcaaaatcaa tatatttcca ttgcaaagcg taagtctcac
    19021 tttttctatc accaagatac agggtaatca taaaaagtgt gtaatcatga aaagtaagta
    19081 aatgattatt aaaatcatca tttactgctt ctatccaatt agtaagttct tttgatgata
    19141 gtgcttgctt accttcttgt attctttttt cttctaaagc atttttttta ggtgcagtga
    19201 tactttgaat aattttagta gttcgattaa attcgatata atttagtatt tctgcaatat
    19261 cgaacatgga acgaacatat ccttttatga tttttatatt tgcatattct ttcgattttt
    19321 tggttaattc atctgatatt atctcagtat tgatatttag ataattcatt gcgtattcac
    19381 caaacatagg taa
//
LOCUS       DAZULY010000023        19351 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000023 DAZULY010000000
VERSION     DAZULY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.023"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(407..766)
                     /locus_tag="ACG81A_000585"
     CDS             complement(407..766)
                     /locus_tag="ACG81A_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFS5013979.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="ACG81A_000586"
     CDS             complement(796..1137)
                     /locus_tag="ACG81A_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013980.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="ACG81A_000587"
     CDS             complement(1134..1808)
                     /locus_tag="ACG81A_000587"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS5013981.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ACG81A_000588"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ACG81A_000588"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFS5013982.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="ACG81A_000589"
     CDS             complement(4322..5512)
                     /locus_tag="ACG81A_000589"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS5013983.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="ACG81A_000590"
     CDS             complement(5533..6060)
                     /locus_tag="ACG81A_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFS5013984.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="ACG81A_000591"
     CDS             complement(6107..8896)
                     /locus_tag="ACG81A_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFS5013985.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="ACG81A_000592"
     CDS             complement(9043..9243)
                     /locus_tag="ACG81A_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5013986.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="ACG81A_000593"
     CDS             complement(9696..10016)
                     /locus_tag="ACG81A_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013987.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="ACG81A_000594"
     CDS             complement(10519..11637)
                     /locus_tag="ACG81A_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013988.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            11870..11992
                     /locus_tag="ACG81A_000595"
     CDS             11870..11992
                     /locus_tag="ACG81A_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5013989.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            12108..13415
                     /locus_tag="ACG81A_000596"
     CDS             12108..13415
                     /locus_tag="ACG81A_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFS5013990.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="ACG81A_000597"
     CDS             13536..14735
                     /locus_tag="ACG81A_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS5013991.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="ACG81A_000598"
     CDS             complement(14762..16030)
                     /locus_tag="ACG81A_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFS5013992.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     regulatory      complement(16086..16152)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(16200..16841)
                     /locus_tag="ACG81A_000599"
     CDS             complement(16200..16841)
                     /locus_tag="ACG81A_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFS5013993.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="ACG81A_000600"
     CDS             complement(17250..17753)
                     /locus_tag="ACG81A_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5013994.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="ACG81A_000601"
     CDS             complement(17876..18640)
                     /locus_tag="ACG81A_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFS5013995.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="ACG81A_000602"
     CDS             18748..19023
                     /locus_tag="ACG81A_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFS5013996.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
BASE COUNT         5542 a         4126 c         3444 g         6239 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgccatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca t
//
LOCUS       DAZULY010000024        19341 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000024 DAZULY010000000
VERSION     DAZULY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.024"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            115..843
                     /gene="purC"
                     /locus_tag="ACG81A_000603"
     CDS             115..843
                     /gene="purC"
                     /locus_tag="ACG81A_000603"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFS5013997.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            843..1097
                     /gene="purS"
                     /locus_tag="ACG81A_000604"
     CDS             843..1097
                     /gene="purS"
                     /locus_tag="ACG81A_000604"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFS5013998.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1099..1770
                     /gene="purQ"
                     /locus_tag="ACG81A_000605"
     CDS             1099..1770
                     /gene="purQ"
                     /locus_tag="ACG81A_000605"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFS5013999.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1771..3993
                     /gene="purL"
                     /locus_tag="ACG81A_000606"
     CDS             1771..3993
                     /gene="purL"
                     /locus_tag="ACG81A_000606"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFS5014000.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3978..5417
                     /gene="purF"
                     /locus_tag="ACG81A_000607"
     CDS             3978..5417
                     /gene="purF"
                     /locus_tag="ACG81A_000607"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFS5014001.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5440..6492
                     /gene="purM"
                     /locus_tag="ACG81A_000608"
     CDS             5440..6492
                     /gene="purM"
                     /locus_tag="ACG81A_000608"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFS5014002.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6489..7067
                     /gene="purN"
                     /locus_tag="ACG81A_000609"
     CDS             6489..7067
                     /gene="purN"
                     /locus_tag="ACG81A_000609"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFS5014003.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7064..8599
                     /gene="purH"
                     /locus_tag="ACG81A_000610"
     CDS             7064..8599
                     /gene="purH"
                     /locus_tag="ACG81A_000610"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFS5014004.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8615..9889
                     /gene="purD"
                     /locus_tag="ACG81A_000611"
     CDS             8615..9889
                     /gene="purD"
                     /locus_tag="ACG81A_000611"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFS5014005.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10011..10925
                     /locus_tag="ACG81A_000612"
     CDS             10011..10925
                     /locus_tag="ACG81A_000612"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFS5014006.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11058..12623)
                     /locus_tag="ACG81A_000613"
     CDS             complement(11058..12623)
                     /locus_tag="ACG81A_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5014007.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12616..14067)
                     /locus_tag="ACG81A_000614"
     CDS             complement(12616..14067)
                     /locus_tag="ACG81A_000614"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5014008.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14081..15097)
                     /locus_tag="ACG81A_000615"
     CDS             complement(14081..15097)
                     /locus_tag="ACG81A_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5014009.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15317..16405
                     /gene="chvE"
                     /locus_tag="ACG81A_000616"
     CDS             15317..16405
                     /gene="chvE"
                     /locus_tag="ACG81A_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS5014010.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16459..17985
                     /gene="mmsA"
                     /locus_tag="ACG81A_000617"
     CDS             16459..17985
                     /gene="mmsA"
                     /locus_tag="ACG81A_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5014011.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            17987..19192
                     /gene="mmsB"
                     /locus_tag="ACG81A_000618"
     CDS             17987..19192
                     /gene="mmsB"
                     /locus_tag="ACG81A_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFS5014012.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
BASE COUNT         6181 a         3288 c         4435 g         5437 t
ORIGIN      
        1 taaatgtaca tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa
       61 acgaacaaaa aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag
      121 aagaaagaac ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac
      181 ttgatttgga tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag
      241 ataagtggaa aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact
      301 caaagaggaa taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa
      361 aaagtacaaa ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca
      421 aaacgtctag gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat
      481 aaggatgacc agttggatga tccatttatc aatgaagagc atatctattt cttacaactt
      541 gcaaaaccgg aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg
      601 accgaattat ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg
      661 tcgaatggac aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat
      721 gaacgtacga atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc
      781 ccagtctatc aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta
      841 tgatgtattt cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg
      901 aggcagtaaa aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag
      961 gaaaatactt tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa
     1021 cgatttgtga caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa
     1081 aaatggagga agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac
     1141 gcagatatgt tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat
     1201 gatgagtcct ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt
     1261 gactatttga gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt
     1321 tttgctgaag aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa
     1381 gcaggacttt tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact
     1441 gtaccattga aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata
     1501 atctatcttc cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa
     1561 gaactaaaaa aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg
     1621 gaaaacattg cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca
     1681 gaacgtgcag tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt
     1741 ttaaaaaact tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa
     1801 gagatcaaga aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg
     1861 atcgaagaag atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt
     1921 atgtggagcg aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact
     1981 agtggacctc atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat
     2041 ggactagcag ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat
     2101 gaaggagctg cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt
     2161 ccaattgcgt tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat
     2221 ttactagaag aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact
     2281 gtaggcggcg aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg
     2341 tgcgtaggtt tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat
     2401 tcgatcatgt atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc
     2461 tcagaagaat ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct
     2521 tttatggaaa agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct
     2581 ctaataggta tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct
     2641 tcaaaagctg gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg
     2701 atgacacctt atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa
     2761 tcaggagagg aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact
     2821 atcggtaaag taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca
     2881 aatctgccag tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa
     2941 ccagctcgaa tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct
     3001 ggacaagtat tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac
     3061 gaaacttatg attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct
     3121 gtgatgaggg ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc
     3181 tatctgtatt tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat
     3241 attattgcta gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct
     3301 gataaaccag aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt
     3361 gaaacgttag gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt
     3421 caagcaatct atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat
     3481 atcactactc aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt
     3541 gcagattttg atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc
     3601 cgaaactttg atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa
     3661 aacggattga ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca
     3721 gaatcagctt tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa
     3781 ttatttgcag aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga
     3841 ttcgaagaaa tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata
     3901 atcgaaattt cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc
     3961 tgggaggatg caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct
     4021 tcggtgtttg gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc
     4081 agcatcgagg acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc
     4141 atcgagattt ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa
     4201 caggagaagc tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca
     4261 atattcagcc ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta
     4321 acttgatcaa tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt
     4381 ctaactccga tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt
     4441 taaataaatt aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga
     4501 cagaaacggc aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac
     4561 aaatgaaaaa cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg
     4621 cacgttttat aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc
     4681 agattgattg ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt
     4741 ttgccagacc agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg
     4801 gacggaattt agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt
     4861 cttctttgtc agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat
     4921 tagtaaaaaa tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc
     4981 aaggtgttcg aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta
     5041 tggtcgatga ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag
     5101 aagcaggggc aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt
     5161 tttacgggat tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg
     5221 aaatcaaaga atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg
     5281 aatcgatcgg tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg
     5341 gagattatcc gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa
     5401 aagtatcgtt cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca
     5461 tacgcaaaag ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag
     5521 catgtcaaaa aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt
     5581 tttgatttat cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg
     5641 gggacgaaat tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc
     5701 gtagcgatgt gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac
     5761 tacttagcaa cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca
     5821 gatggatgtg ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt
     5881 atgtactcag gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct
     5941 caattattga caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc
     6001 gggattcatt caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt
     6061 tctttatcat caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca
     6121 acgaaaatct atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct
     6181 gcacatatca ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg
     6241 gctgcacaaa tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa
     6301 tatggtgaaa ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc
     6361 ctggcagtcg aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa
     6421 aaagtttatg aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga
     6481 gaagaacgat gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag
     6541 ccgattatct atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc
     6601 ctgaagcata tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac
     6661 ctaaagaatt tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa
     6721 aaaagatcga tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga
     6781 aaaattatga taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt
     6841 tgcatggcat acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt
     6901 atatcgacca aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac
     6961 aggaggacac gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc
     7021 cagaagtgat tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat
     7081 cagtgtatca gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt
     7141 agaaatcatt tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct
     7201 ttcgatcgaa gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca
     7261 tcctaaaatc catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat
     7321 ggaaacggaa aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga
     7381 aacgattatg aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg
     7441 tcctagtatg ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc
     7501 atctgactat gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt
     7561 cagacaaaaa ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc
     7621 tcaatactta acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga
     7681 acgaaaacaa acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc
     7741 cgctcttctg gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc
     7801 ttataataat atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc
     7861 aacggtagtc gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt
     7921 agaagcgtgg gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt
     7981 tttgaatcga gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat
     8041 catcattgcc ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat
     8101 ccgattactg acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc
     8161 agtcttaggt ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg
     8221 gaaagtcgtg acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg
     8281 gagagctgtc aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt
     8341 aggaatcgga gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc
     8401 gcaagagaaa atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc
     8461 ggttgaatat gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa
     8521 agaccaagat tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt
     8581 tcggcatttt agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg
     8641 tggtcgtgaa catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta
     8701 ttgtgcgcca ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga
     8761 gcgagatcat tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt
     8821 tggtcctgaa gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa
     8881 aatcttcggt ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca
     8941 attaatggat acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa
     9001 agcagcagcg tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc
     9061 agctgggaaa ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca
     9121 aatgttgaac cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt
     9181 ggcaggagaa gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt
     9241 catttcgcaa gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat
     9301 gggtgcttat tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga
     9361 agttttaaaa ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt
     9421 atatgcgggt ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt
     9481 cggtgatcct gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat
     9541 cgatgattta ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt
     9601 aggcgttgtg gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc
     9661 agattttctg gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa
     9721 actagttgga gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga
     9781 agcacagaaa aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg
     9841 tacagatatt ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt
     9901 ccatttataa aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca
     9961 taaagagtgt ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa
    10021 ttcaattagg tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca
    10081 acaatgcgga agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt
    10141 ttgaccatgc agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag
    10201 caaaaacatc aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag
    10261 gtgtgatgtt tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc
    10321 gtctgaaaat ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac
    10381 cagaggaagt agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg
    10441 gcgtcagcaa tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat
    10501 tagtagctaa tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc
    10561 atgtcaatat gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc
    10621 gattgcatga tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg
    10681 tatttatcgg gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta
    10741 aatatcaaac gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata
    10801 tgcaagtgat cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag
    10861 aaattaatat gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac
    10921 cttaaagatt gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga
    10981 aagctgtttt cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat
    11041 tgtacggatt tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct
    11101 tttggagaaa tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa
    11161 ccaatttcaa aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct
    11221 aattcgattc tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa
    11281 atcgtactga aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata
    11341 tctggatcgg taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat
    11401 ttgttccttg aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc
    11461 gaacggtaaa gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga
    11521 accatttctt gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag
    11581 acaaataaac gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg
    11641 atgtacttag atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa
    11701 aagacagaat caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt
    11761 tctttaaggg aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat
    11821 tcgctgattc tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt
    11881 tctaattcat ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca
    11941 agagaatcaa ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt
    12001 tgattctccc taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct
    12061 gtactgactt ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg
    12121 aaagattcag caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc
    12181 aagaaatgat tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc
    12241 tgcaaatcta aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc
    12301 ggtttaagca gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg
    12361 tcgaatccac taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa
    12421 aaagtcaacc cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag
    12481 tcgataattg aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga
    12541 taataagaac caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct
    12601 tcgatgataa aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat
    12661 catcagtgtg acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc
    12721 atttccgtaa taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa
    12781 atctgaatcg atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat
    12841 gccaatcccg ttgtctataa ctttgaattg gatcatctca tcaattttgc atgcggtaat
    12901 acgaataaat cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa
    12961 tggctgcaag atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt
    13021 atagtcaagc atatggccgt atcttatttt taagatgact agataatcat tgatatggct
    13081 aatttctttt tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc
    13141 ggaaagtgca tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat
    13201 aatggcatcc aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac
    13261 ttcactttgt gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc
    13321 ttccgccatt ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt
    13381 taaacgaaag ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat
    13441 cggttcttgg acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt
    13501 actaagaact ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt
    13561 ttttttgctt gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag
    13621 ttgagtgaca gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc
    13681 caagatatta ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga
    13741 tacatctaac gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc
    13801 attcctaagt tcttctagga tattttcctc attgaactca tctacggtta cttggccaga
    13861 aatcaagtcc cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc
    13921 tttttggata tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat
    13981 gctcagtagc aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat
    14041 ctgttttatg cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga
    14101 tgttagctat attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg
    14161 tttttcctgt ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga
    14221 catcaggttt acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat
    14281 cttcttgtcc atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt
    14341 ttatcgcagc taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt
    14401 cggataaatc atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa
    14461 aatcaccgac aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt
    14521 tttttagggt tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg
    14581 gtgtggagtt ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg
    14641 tgaagtgatt gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa
    14701 tgtgggtgac gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag
    14761 ggatccctgc tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag
    14821 taaaaatgat catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt
    14881 cctgattcat gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga
    14941 taattgattc agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa
    15001 cgactagtgg gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga
    15061 ttacaacaag ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt
    15121 gttagtacta tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa
    15181 aatccaagaa acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa
    15241 atcccataaa aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg
    15301 aaatggggga taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt
    15361 gaccgcaagc attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg
    15421 gtatgtagga atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa
    15481 catggtctcg gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa
    15541 agtagaaaat caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt
    15601 gattgcttct atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga
    15661 tatcaaggtg atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc
    15721 gacttttgat aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg
    15781 attgaaagaa ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa
    15841 caacgcacta atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg
    15901 tcagttggtc gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga
    15961 tggttctacg gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac
    16021 attggatgct gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa
    16081 aggcgtgggg tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac
    16141 agttgctggc gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac
    16201 acgtatctta gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt
    16261 tccggtcaat gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc
    16321 aaatacagta tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta
    16381 taaagaatcc gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc
    16441 agagagagga taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa
    16501 ttttctggaa tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat
    16561 gcattgtgcg gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg
    16621 tatccatatg gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa
    16681 gatttgaaag atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt
    16741 ccttatcttt caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg
    16801 attgattggg aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct
    16861 atcgacccga atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata
    16921 gctaaagcat tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg
    16981 aatgaagaag aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta
    17041 acctccatca ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc
    17101 attttacggg atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat
    17161 attattcgcg ggatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat
    17221 attggagaag tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag
    17281 cgaatcataa acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct
    17341 ggactgatgg gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga
    17401 agcaagatat ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct
    17461 tcagccattg caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat
    17521 ctgctgatgg atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa
    17581 ggagtattag ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt
    17641 ataaaaacaa actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa
    17701 gtcgttttat ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc
    17761 cgtgggatag atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca
    17821 aatggcaaag ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat
    17881 cgaatctata cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat
    17941 caggaagtct tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa
    18001 aaatcttacg gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt
    18061 tggtcgctac agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac
    18121 ggatggtatt ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat
    18181 attagtacta gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt
    18241 agggtcagtg atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat
    18301 gaacccgtgg atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca
    18361 aggtttttgg gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct
    18421 gatgtttcgt ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc
    18481 tgttttccaa aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca
    18541 cttcttgaca ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg
    18601 aacaagaaat cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt
    18661 aaaggcggta tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca
    18721 agggtatccg attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac
    18781 aaataaaaca gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga
    18841 actatcaggg atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat
    18901 ggcagcttta gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg
    18961 tacatcgatg gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg
    19021 cggcattgga acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa
    19081 tggcatgtcg attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt
    19141 gcttttagcc gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc
    19201 cggttattca gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag
    19261 cgaacattca ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt
    19321 ataaattctc tttttgctct t
//
LOCUS       DAZULY010000025        19077 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000025 DAZULY010000000
VERSION     DAZULY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.025"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..707)
                     /locus_tag="ACG81A_000619"
     CDS             complement(<1..707)
                     /locus_tag="ACG81A_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS5014013.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQA"
     gene            complement(1068..1138)
                     /locus_tag="ACG81A_000620"
     tRNA            complement(1068..1138)
                     /locus_tag="ACG81A_000620"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1104..1106),aa:Gly,seq:ccc)
     gene            1255..2007
                     /locus_tag="ACG81A_000621"
     CDS             1255..2007
                     /locus_tag="ACG81A_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS5014014.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            2023..2502
                     /locus_tag="ACG81A_000622"
     CDS             2023..2502
                     /locus_tag="ACG81A_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFS5014015.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(2753..4333)
                     /locus_tag="ACG81A_000623"
     CDS             complement(2753..4333)
                     /locus_tag="ACG81A_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFS5014016.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(4326..5069)
                     /locus_tag="ACG81A_000624"
     CDS             complement(4326..5069)
                     /locus_tag="ACG81A_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5014017.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(5245..6021)
                     /locus_tag="ACG81A_000625"
     CDS             complement(5245..6021)
                     /locus_tag="ACG81A_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS5014018.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(6269..7105)
                     /locus_tag="ACG81A_000626"
     CDS             complement(6269..7105)
                     /locus_tag="ACG81A_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFS5014019.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(7116..8045)
                     /locus_tag="ACG81A_000627"
     CDS             complement(7116..8045)
                     /locus_tag="ACG81A_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFS5014020.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            8237..8590
                     /locus_tag="ACG81A_000628"
     CDS             8237..8590
                     /locus_tag="ACG81A_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS5014021.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(8664..9320)
                     /locus_tag="ACG81A_000629"
     CDS             complement(8664..9320)
                     /locus_tag="ACG81A_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS5014022.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            9546..11480
                     /locus_tag="ACG81A_000630"
     CDS             9546..11480
                     /locus_tag="ACG81A_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5014023.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(11566..12789)
                     /locus_tag="ACG81A_000631"
     CDS             complement(11566..12789)
                     /locus_tag="ACG81A_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5014024.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(12783..14654)
                     /locus_tag="ACG81A_000632"
     CDS             complement(12783..14654)
                     /locus_tag="ACG81A_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFS5014025.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(14675..15793)
                     /locus_tag="ACG81A_000633"
     CDS             complement(14675..15793)
                     /locus_tag="ACG81A_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5014026.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(15876..16709)
                     /locus_tag="ACG81A_000634"
     CDS             complement(15876..16709)
                     /locus_tag="ACG81A_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5014027.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(16702..17619)
                     /locus_tag="ACG81A_000635"
     CDS             complement(16702..17619)
                     /locus_tag="ACG81A_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5014028.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(17642..17821)
                     /locus_tag="ACG81A_000636"
     CDS             complement(17642..17821)
                     /locus_tag="ACG81A_000636"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014029.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(17846..18970)
                     /locus_tag="ACG81A_000637"
     CDS             complement(17846..18970)
                     /locus_tag="ACG81A_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5014030.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         5949 a         3764 c         3191 g         6173 t
ORIGIN      
        1 gcttgtcctg tctcataatt gatcgtgtag ccatcttgaa cattgtagat aaagacattg
       61 aattcaattt ttttgtcttc aatgctttct gcttccaatt gcactcccct ggctaccagt
      121 tcattcccct caaaatgagg agtgacacgg tagcgtacat gatgatttgt ttctttgata
      181 tatgccgcaa tatcattttc gtgagcaagc atgtatggag cattcaacga acgtgtccct
      241 gtcatcaagt ttttaatgtt gttattttga ccagtcagct ggaaaccaat taaatggctg
      301 cgattatata accattgccc gtcacttaat ttcttttgtt tccaacctgt cgggtcaata
      361 tacaaaggct ctctcttttc agtagggaac aaatcttttc caagcatagc attggcttgt
      421 cctactctgt tcaatttatc aatatttgaa tagctttccc atgttccttt ggaaaggcta
      481 agatcctctt ttgtaaattc cggctgattc ccattgattt ctatttcttg atgttttcca
      541 tcatattctg gcacatccgc tacttgagaa gcttgtggtg tggaatcaaa aatagttgta
      601 atctgctgaa agatatctga atctttttgt gtggtggaaa caccgaatgc ccctgcaatc
      661 acagcagcga cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt
      721 tactaacagc tccctaaatc ttaacatatg ttcccctata aataaatact agaaagaaat
      781 agaaacagca aaaaataaaa cctgttttaa gatggcttaa aaatacaaaa aagagacgga
      841 ttcctcgtct ctaatcagtc accactctat ctgtaaggta tttattatta ttcaaaaatt
      901 ttcttcatac tcaagacaat acactgattt cacaaccgct tggtaaacgg tgcgcaaaag
      961 cttgcatctg cggtaataag tccatccaag ttaaaaacat aagaagttgt ttttgcttgg
     1021 ctgttcttat tacttgttgc agagcagctt tttcacaacc tattttaagc gggtgacggg
     1081 aatcgaaccc gcgacatcag cttgggaagc tgggatttta ccactaaact acacccgcaa
     1141 tacaaaaaca gtatacgcct cttttattcc ttcctcaagt ttttttattc tttacgcttc
     1201 tggtatgttt tgtagtaaaa taaaactaat caatcaatta ttggagaaga tgctatgacg
     1261 ggaaaagaat tagatctgtt gctggacact tgtttactcg ctgggaaaat catgatggaa
     1321 agcaatgcgg agatgtatcg ggtagaagat acgatgagtc gaattgcttt agcttctgga
     1381 aatttccgtt tggtcagcta tgtcactcaa accggtctct ttattggtct tgatcgtacc
     1441 tcgaccatcc gaatggaaca aattaccaac cgttcgatta atttagaaaa agtagtaaaa
     1501 gtaaatgatc tttcacgtaa atatgttgct tcagagctaa cattagaaga actttactgc
     1561 gaactgcaag caatcgaccg agaccgttct ttctttcctg tctggttgca aatcgttagc
     1621 gcggcagtga tcagtggaac aattatggtg ttatttggcg gaaagatgac cgatttacct
     1681 gtgactttag tcattggagg attgggatac gcagcttatc tatatagttt gaaatttttt
     1741 agaatcaaat ttttatcgga atttttatct tctttattga ttggcatagc cgctatctac
     1801 agcgtacgtt ttggcttagg aagcaatcag gatctaatca ttatcggatg tgtgatgcct
     1861 ttagttcctg gagttcaaat tacgaacgct atccgtgatt tgctagctgg acattatgtc
     1921 tcaggagttt ctcgcggaac agaagcgatg atgactgcta cgatgatcgg ttttgcgatt
     1981 gcttttattt tccagctttt ttattaggaa aggaacacaa gtatggggac gatatttatt
     2041 cagttttctt ttagtttttt agcgactgcg gcatttgccg ttattacaaa tgtacctagg
     2101 cgttcattga tttgttgtgg tctttctggt gcttttggct ggatgatcta ctggatctgt
     2161 gttcaaatag gcggatctgc tgcttttggt tcattgcttg gttctttagg agtagcattc
     2221 atcagcgatc ttttctctaa acgtttgaaa atgcccgtaa cgattttcaa tattcccggg
     2281 atggttcctc tagtccctgg tggtttagct tatcaagcgg ttcgaaatct cgttactgaa
     2341 tcctatcgag aagcggctca ttatgcggtc caagcaatta tgattgctgg agcaatcgct
     2401 ttaggtcttg tattatcaga agtctttaac cataacatcc gtaatttcaa agtaaagcgt
     2461 gacgcaatca tgatgaaacg aaaaaaacgg ctttttaaat aaacgaagtg taacaaagaa
     2521 aaggttgtga aagagacgtt cagccttgag cgatgacaaa atatctcaca aacattgcga
     2581 caagtaaccg caggagcaat aagaagtaat tatgagtatt aaaaaaagtt ttgtaaaaat
     2641 agcttctcac atttttacat aaaattgact taatactgag aaactgactt ttgaacaccc
     2701 tctatctgta aaaaaggggc tgcgacatga tttttgtcac ggcccctctt ctttattcaa
     2761 attttttcca aaacgttcgt tgataataaa cgatccatag agcggaaagt attagaacta
     2821 aaccgatcac aggaatattc actaaccaaa atggataaaa caaagccaac attccgtata
     2881 agacgataag aataatactg atgatcaatg aacccatcat agttgcgact aaacccaaac
     2941 ttcctgaacc tctgttaaat aattggttaa tattggtcca gttcagcagc agcaaccgat
     3001 aatctctagc gaaataacgt agtgacaata agtagcagcc gaataaagaa ccaaatagca
     3061 agctagggat aaaaaacaaa ggcagctgaa aagacagtcc gcttaataat gcgatcccac
     3121 cagttaaaaa actctgcaat atccatccga atctgaactt ttcttttaaa tattggttca
     3181 tagagattgg caaagaacgg ataaacagaa aattttcttg atccaaagaa ataagattgc
     3241 tgataaaaga agtctgatta actgttaaga aagccaatgc gatccctacc aggaaaacaa
     3301 caccgattaa cttattatct aaatcagtta attttatttc gcctcctagg gcaaaagcaa
     3361 gaataaaaat gattggagtt aacaaggaag aagaaagaac ttgcataatc aaattaggtt
     3421 cttttactaa ttgagcattg taactaaata aaagctgatg cacattttgg ttctttttat
     3481 gggttctttt tattttgaac tcactgtttg aatcagtcag ttgctctgta agctttggta
     3541 ctatcagctg ttttacagca aggaaaaatc ccagagccgt gatagctagt attccccaac
     3601 ccaataaacc ttgttgagaa agtggctgac tggcagcatc atagaatggc aataatatcg
     3661 aaatcggatg ccgatctgtt aactcatttt caatagtccc cgtttgatga ttcatccaca
     3721 tgattccagc aaatgcgatc agcatcgagc tgccaagcaa gatactagaa actgtttttc
     3781 tatgtttttt aaacactgct gttcgagcca aaccaaaaac aatcaaacta gccagacaaa
     3841 aaagaataat caaaaataga gaaaacaaaa ggattgagat aatgatagta agcaaaatcc
     3901 ctagatcagc tcgccatccg gttaaaataa acatgactag aagcggaaaa acaaaaggaa
     3961 tgatggtcaa agcaactacc aaaatctttg ctgaaaaaac ctgttcttgt ccaaaaggca
     4021 gaggcaaaaa tacttgcagg tcatttcctt caaaaaagat attataaatg actgatatac
     4081 tttgagttaa acctaacata ccaaaaagtg ccacgtaata agtaaagaaa cctggcaatt
     4141 taacaaagtt catagcaaac atagcaaatc cgtaaatcag taaaaaaatc agccctgaaa
     4201 aaagattttg agacaaaaga tagatagtta atcgctttcc cttttttcct ttctctcttg
     4261 ctttattcgt gagctgtgga ttaacatata aaagattaac tcgaattaat tctaataatt
     4321 gtcgcttata catacggtcc atctcctgtc gcttgccttc cagccatttt caaataaatc
     4381 ttttctagcg tttcatcagg tgagccattc aataattcac taactgcccc ctgataaatc
     4441 aattcacctt tcttcaaaat agccagttta tcgcataact gttgtgcggt atccaataca
     4501 tgtgtagaaa aaatgactgt ttttcctttt gcggcatgag ccttcatcat ttctttcaaa
     4561 tcaaatgctg cttgaggatc aagtccttgc aaaggttcat ccaataccca aatatctgga
     4621 tctggcagca acgtgccgat caaaatcgtt ttttgacgca ttccatgtga aaaacttgcg
     4681 attgtttcat tttgatgact atacatatca aaaagcgctg tcaattctgc taaacgcttt
     4741 tctttttttt gtgtgtctaa ttcataggca gctgaaatca agtcccaata ttctccagcc
     4801 gtcaattgta aaaaaatatc tggggaatca ggcacataac cgattttttg tttgatactc
     4861 aatcgatttt ccgtcaattt cattccatct actaaaatcg ttcccgatga tggttcaata
     4921 atactaacta aacttttaat cgtagttgat tttccggcac cattatgtcc taaaaatcca
     4981 aaaatttccc cttgtcttat cgagagagac aagtctttta atgcttgttt tttcccgtaa
     5041 cttttactta cattgatgaa ttcaatcatt tctgtactcc ttttgtttgt tattactttt
     5101 tattgtaccc ttcgttattg gtggagtaaa tgtcaaaaag agtgcataac aaaaaaggat
     5161 agaaacgact aggctgctac ttgggttgtt ctcttctaca ggaaaacact cttctaacag
     5221 gtaagatatg agtctttgtt ttctttaaaa tgattcattt tctatctttt cttgcataaa
     5281 attctttagt ttatcaatac ttctctttcc catctctagt tgttctccat ttgcaaatgt
     5341 tactatttcg tttactctgc taatagatgt aatgttttca gtattaatga tataagcttt
     5401 taaatcttta cataaatact catattcgag tgatttttta tattttttaa ttgtgccgtt
     5461 tacgagaatc tcttcatctt tcaatactag gcctatttta tttttataac ctggcatgct
     5521 cattagataa aggatttcat cgtaatggat atagtgatcg ctattaccat aacggacagt
     5581 tgtccacctt tttttcgtag catgttgaac aggatagttt tctttttctt tcaataagta
     5641 actttggatg ctttttttat ttttttcata agtgaattct tttatcaaat aaccactagg
     5701 cgtaatcttt cgattaataa cagttattgc tttattcgga tgagacgtta gataaacaat
     5761 tttacacttt tcattctttt ctctaagcat ttcgcctagt tcgataccgc tataataggc
     5821 gttcaaatcg atgtctagga aaaaaatatc tgaggaatag atttcattat ttttaatgaa
     5881 ttcaacaaat tgtataacgt tcaatccctg aacgatctca aatttccaag gacttaataa
     5941 aattctggat tcttccaaaa tttctactag cattttttca aaaaccaaac tatcatccaa
     6001 aataaatata cgcgttacca taaaaagtac ctccactcga tattttacta tcaaaatcat
     6061 ttcatacaac ttttcaacat acatgttagg aataaattgt aattctcttc acaatctttt
     6121 ccaacacctt tgaaaccgat atcaaatact ttcaggatgc tgcttctaaa ataaatatca
     6181 aacaaatgaa tacatcatgc aaaaaaagcc agacaaccga cgtccttgcg tgtcagctgt
     6241 ctgagctatg tttatttatt ttgttctttt aaaaagaacg atacacatac tgatttttcg
     6301 gagcagagac tttctcagcg tttgtaacaa ctacagatgg gatttcacgc ttgaatggtt
     6361 gataatagtc tgattggatg gttccttcta ttttatacca ttcatcattt ttaaccgtta
     6421 ctccttccgg catatgcgtc aacaaaccaa agactcctga atctgccaca caatggataa
     6481 tgccgaatcg gaacaagaag atatctgtat tgctatcgtt cggtgtttgg taaacaaatc
     6541 cttcatagct gatttttttg ccaatgaatt cgctagggta attataaatt agctccataa
     6601 cttctaaata gttttcatct gtgatcttga tagtttgctt atccccatac ttttctttca
     6661 actcattcat ttggtctaag tagtctgatt tgttgaaata aatactggta tccggcttta
     6721 agtattgtgt ctgcatctcc ggatccccta ctgattcttt acttactggg aaattaaatc
     6781 ctttggcttc tacgatcgta gtgtcgagac taactgttgg aaacaacgtc cccacgatta
     6841 ctggcaaagc tagcagtaca taagcgattc cacgttgata aggttttgac aacccatggt
     6901 catgatcgtg ctcgtgtgcc ttttcttctg cttttccgtt tttgacccac aaatacagct
     6961 gaactaaagc caaaagagca gataaaatca ttgaaagaat agctaagtag cgataatgga
     7021 cattgatata ttgatcgagt ttgccagaaa cttgcaagta catcatcaat tcgaaatacc
     7081 cagataaaat caaaaaccgc agcatatttt ccctcctaaa taaataatgt gaatccaata
     7141 acgctaaggg caatcagtcc gatcagcgat aaaataaagc gtcctgtaaa atatcgtttc
     7201 atcatcagta gatttttgat atcgaccatt gggccaaaga ccaaaaaggc agctacaggt
     7261 gctgtgccga acagacttaa caaagaagat cctataaacg catctgcttc tgagcaaaga
     7321 gacatcgtaa aggccaataa cagcatgatg ataatggcta aaatctttgt tgagccgagc
     7381 gttagcataa ttctagttgg taaataagtc tgcatcgcag ctgcgattaa tccgccaatg
     7441 atcaaataac gacctgtatc aaaaaattcg tcgattgaat gtgtcaacac atgcccagtt
     7501 tgatggatca gctgattttt tggttcattc ttctcacttt gttcttcttc atggacatga
     7561 tgatggtgat tttctgctac tttagacgtg attttttctt tcaatacagc gtctttttga
     7621 aaataggcaa gccagacacc aatcacaagg gcaacaaaaa agctgcctat cacccgccag
     7681 cttgccatct gccaagaatt gccaaatgcg ataaaagtgg aaaaaatcac gattggattg
     7741 atgatcggag cagtcagcat aaaagcaaac gctgtgtgaa ctggtacgcc ttttttaaca
     7801 aattgatgaa cgatcggtac gatgccgcat tcacatgaag gaaagaaaaa tcctaagatc
     7861 gaaccagtaa aaatagagaa gatacgattt tcaggaagcc atctcttcac acgttctggg
     7921 gtcaaaaata cttgtaaggc gcctgagata agacagccta ataatacgaa aggtaatgct
     7981 tcaataacga tcgataagaa aatcgtactc atctgcaaaa ccgaatttgg aataaaagaa
     8041 aacatgagtt tcctcctttt tatttctagt tcattcgctt tgcaaatata cgcttttatt
     8101 caaaaaaaac aagttgctct gtaaattttt acacaactct tattttttac caaagcactt
     8161 caattgactt tttctgttat tcccattaaa ttaaggtctc atacatatca cttctatcat
     8221 aaaagggaga ataaaaatga aacaaaccac taacttgact gaagttcaag acatactgca
     8281 acagtcatcc gttgccgtca tttatctttc gatgcctaat tgcagtgttt gtcacgctgt
     8341 tcgtccacga ctagaggaac ttctcacaca ttatgatatt ccggcacttc atttggatgc
     8401 atttgaaaca ccagaagtag ctagtaaatt tgaagtgctg actgcgccag ttgtcctgat
     8461 ttttcatcaa ggaaaagaag tctttcggca agcacggttc atcgacttta aaaaaatccg
     8521 ttatctacta gatgaactga cagcagaaga tgattccctt tcttatgaag aaatcttccg
     8581 aacaaaataa tcgttgtcca aacagaaaaa agcgattgaa caagtgaaag ttttgccttc
     8641 atttgttcaa tcgcttttta tttttattct gctgtatttt cggaatgcgc atttgcgtca
     8701 gaatgcaaga aatagatcaa ggtttgcaat tcattcgtca aatcaacatt ttggatcaat
     8761 acatctgacg gagcattcaa tcgagcagcg gtaaagttca agattccttt gattcctgct
     8821 tccgttaact gattgactac ttcttgtgct ctttcagcgg gtatcgttaa aattgctact
     8881 tcgatttgtt ggatcttgat ttgttctgac atatcttcca ttggataaac ggggataccg
     8941 tctacgatgc gcccaacgat ttcctcattc acatcaaaag cacaactgat acgtatgcta
     9001 ttactttgat ggaatttgta ttttagtaaa gcacttccca agttccctac accgatcagc
     9061 gcaacatttg tcaattgatc gtcgtttaat gtctttgcaa aaaagtgcat aagatcttct
     9121 acatcataac catacccacg ttttcctaat tcgccaaaat aagaaaaatc tcgacggatc
     9181 gttgcactat ctacttgaac ggcttcactt aattcagttg aagacacttt tttctttcca
     9241 gcattattta aaatccttaa atagcgataa tacaaaggca gtcttttagc tgtcgccttt
     9301 ggaataatat gatctttcac gaaatgacct ccacgtattg tctaattaca tctttgattt
     9361 ctccatcata gcagtattta taggaaaaaa tcaattccat tgctcagata ttgtgaaatt
     9421 ttattcttta ttcatttatt tatcatttct gcaaatctcg cagtataatg gaatttatgg
     9481 tagactacta attgaaaaat agttttttat ttgaggagaa ttcacaaagt agaaaagagg
     9541 attccatgat attattacaa gccaatcaga ttgctcgcca ttttggtgca gaagtcttat
     9601 ttgaaaacat acatttagaa atcagtacag gcgctcgtgt cgctttagtt ggcagaaatg
     9661 gcgctggaaa atcgacttta ttaaagatca ttgctggaat tgaagcacct gatgaaggaa
     9721 agatcgctaa aaataaaaca gcgactctcg gatacctcgc tcaggacaca ggacttgatt
     9781 ccaatgaaac agtttggaac gagatgctga aagcttttga acaagttaga caaatggaag
     9841 agcggatgcg cgagctagag attttcatta gcgaaggtac gccagacaca cctgcttata
     9901 gttcttattt aaaagaatac gaccggctgc aacacgattt ttcagatcga aacggctatg
     9961 gttatgaaaa tgaaattcgt tccgtattgc atgggtttaa attcgattct tcattttatg
    10021 atcagaaaat cgatacttta tcaggcggac agcgcacacg tttagcttta gctcgcatgc
    10081 ttttgcaaca ccctgacatt ttgattctag atgaaccaac gaaccattta gatatcgaga
    10141 cactttcctg gcttgagaat tatctgcaaa gctatactgg tgcattattg attgtttccc
    10201 atgaccgtta ttttttagat aaagtagtca atgaagtcta tgaattaagc agaagaaaaa
    10261 tgcgccatta caaaggtaac tattcgaaat atctagattt gaaagcagaa cagttagcaa
    10321 gtgaatggaa ggcttatgaa aagcagcaaa cagaaatcca taaattagaa gacttcgttg
    10381 ctcggaacct tgttcgcgcc tctaccacaa aacgagcgca aagccgcaga aagacattag
    10441 aaaaaatgga ccggctggat cgtcctcaag gggatgaaaa acaagcaaac tttctattca
    10501 gtatagaaaa gccttctgga aatgtagtct tgcaaatcga agatgccgct atcggatatg
    10561 ataatgtcac cttatctgaa ccaatcaatc tagatatccg tcgtcaagaa gcgattgctc
    10621 tagtaggtcc gaatggtatc gggaaatcga cgttgctgaa atcaatcatt caccagatcc
    10681 cttttatccg tggaaaagaa acgctaggga caaatgtcac catcggctat tacgatcaag
    10741 gtcaagctga tctgcataca aacaagacga tcctagcaga attgtgggat gagcatccta
    10801 ccactccaga aaaagagatc cgaagtgctt taggtgcttt cttattttct ggcgaagatg
    10861 tagaaaaacc aatccctctg ctaagtggcg gtgaaaaagc acgggttgct ttagccaagt
    10921 tagcgatgaa caaagaaaat tttttgattt tggatgaacc aacaaaccat ttagatattg
    10981 acaacaaaga agtattagaa aatgcattga tcgattatga cggcacactg ctttttgttt
    11041 cccatgaccg atatttcatc aatcggatcg cgacaaaagt cgtcgaactc tctgaaaatg
    11101 gcagcaaagt ctatctagga gactatgatt attatttaga gaaaaagcag gaagaagaag
    11161 aaatcgccga attgttggca caagatgagc cagaatctgc tcctgctccc acgaacaaat
    11221 ctgattatta ccaaagcaaa gaacagcaaa agcttatccg tagccttcaa cgaaaaatta
    11281 ctcaggttga agaagaaatg gcgcggatcg atgaatggat cgatcagttg gaacaagaaa
    11341 tggtcgcgcc agaaaacctg gaagatcacg taaaactcaa tgagctgaac caagatttag
    11401 aagctgctag acaagatcaa gaaacaaaat tgacggaatg ggaagaacta agcttggaat
    11461 tagaagaata tgaaaattaa cgaaaaaacc aaaacagaaa ccattcatgc tgttttggtt
    11521 tttctttttt tattttatca aacaaaaatt ccatatttac ctttttcacc aataagaaga
    11581 ccaattacta gtcattctca ctaaagcttc taaataatag taatcacccc aaatacaagg
    11641 tgtatttacc ccattatttt tgggcttatt ataaacagac tgtgttaaaa agccatcttc
    11701 ttcttccgtt gtagcatatc tttctttcaa ttgtcgcata attttccaaa cagcagactg
    11761 gtataagttc ttttctgttt tgtctaatag acctgattct attaattcta ataatccaca
    11821 aaccatgatc accatactag atgtatcttt ttcttctctg ctagaatcat caaagatcaa
    11881 atcccaataa caaatggtat cttctggcaa ccgattcata aaataattgg ctaatatttt
    11941 gcttaaatca aaatattttt gttctttcaa ataacgataa cttaatgcaa aaccgtaaat
    12001 cccccatgct tggcctctag cccaacagga attatcacta taaccttgat gcgtacttcc
    12061 atatcttggt tttccatctt tgacatccat ataaaatgta tgataagttg aaccatcttc
    12121 tcttactaaa taatctgctg cattttgaat atgagcctga gcagcttttt tatatttttg
    12181 atttccagtt tcttcactcg cccaatacaa taatggaaga ttcatgcaac aatccatgat
    12241 cattctacct tgttgtgaag gatcactagg atctccccaa gcttgtataa ctcctgcctt
    12301 ctcccaaaaa cgctgaagta atgtttctgc agcatcaatt gaagcttcct tcatttttcg
    12361 atttcccgtt attttataat ctgctacggt tgataaagaa tacaaaaaac caagatcgtg
    12421 atgattagtg ataatttgac agtcaatcct gtggataaaa tctatatctg ctttttcagc
    12481 tgtttgttga aataaaggat cacccgtctt ctcaaataac aaccaattaa ttcctgtcca
    12541 aaatccttct gtccaaccaa tattgtctct taatggataa cgtcctttta taggacactc
    12601 gcttgggaat tgatactgac tgttactgat acgtttgatt aacctctccg tgtgtctgcg
    12661 acaacgctcg atctcattat tccattcttt ttccattaat tctttataac attcatttat
    12721 ccatttttct ggatgtatta tctcttcaaa catcacttta tcaagcattt ccccaccagc
    12781 ctttagcata attttagtct ttttttattg tttttatttt ggatagttag cccggttgtc
    12841 ccatcaaaat caattttggg acgattgttc catacttcta aactataatc ccgatcttga
    12901 tgagcaacca ctcctgttcc gagccaatgg gttccttttc caaatgtacc ctctaatcca
    12961 ggaataaaag tgttttccca tgtcatcaag tttgtattgg gcaaacccat aatagctgtg
    13021 gattttcgtt taatagtatc gtcacttaaa cagataatac ctgaaaagcc agcttcactc
    13081 accctatagt tttccttgtc ttcttctatg attattcctt ctttctcagg agaacattca
    13141 atagcaaacc ctgtctcata tagaacatat tctgtatcta attctacttt gtgtacgcga
    13201 atctgccagc cttcaaaagg aataagccaa gttctgattt ttgcctgtaa agtaggttcc
    13261 catgtagagg caagtacctc tttcttacat acaatgtctc gtacattatt tcgaacgatg
    13321 aactgtccat aggttttgat gctctcattc ttaaaactgg tcgtgatata tgtgtccgct
    13381 cgctgaatcc ctagcattga atccattgcc atttctgctt tcaattgatt cgctcgcggt
    13441 atcgaaaaac caaattgact agaataagca aattttaaat atttttcatt ggtcaatgta
    13501 tggaaatttg gccctaattg tcccgcattc aaaaaaaatg tatgtccatt atcatgttgt
    13561 atgagcaaat tggcttctga aagaagttta gttccttcac gaataggcat aggcatttct
    13621 ttagccgtcc aatactcatg gtcttcaggt agcgccaaca ataaaaaaat cttattcagc
    13681 cataatggag ataacgtact attatagggt tcagtcaatg tcaattgagg gtaggtataa
    13741 ccaatattca ggataccttt ttcatcaaat atttcttttt gcatccacca tcttatattc
    13801 cgattgacta accccttgat ttcacctaat ttaaaaggca aaagatcggc ccacaacata
    13861 gctgaccaaa agcttgaaac tgcaaaacga taaatcatgc ttcgaccaaa aggcacattt
    13921 gcaccatctt ccgcaaaaaa atgaatatag tctctagaaa aatcaatagc tcggttctta
    13981 tatatctggc taatgggctt ttctggttct agtacgctat aaatcaaacc ataataatga
    14041 aatgcaaaag gattataata atcttggcga cctcttgaac tgtctcgata ccagccattt
    14101 tgaagataac aattttctat attatcaaat gcttctttca gtgctgattc tttaactgga
    14161 taaccaaatt tttggataat tgttccgaca attatcttaa aaaattgcca attaccatct
    14221 gcacattcat attcaaaaat ttgcgagaac caatgaataa tctggctttt ttctcctgta
    14281 tctaattttt tccataataa ttgttcatga tgatataacg ctaataacat tgcgggcatt
    14341 tctaccatac gctggtctct ttcatgtatt tctccccaat aatcgggatg ggaaggatct
    14401 gttccagctt ttattcctga tagaattaat ttcaaccatc gttcatctaa ttggtcaatt
    14461 gcgggccccg ctccccataa aacccgagag aatccttcca ttcctgcaat tttatttcca
    14521 tatcctaccc cttgatgtat ataatcaatt tgtgcgccac ttgctcgata aaacggctgg
    14581 agtggttgat ttatcttttt aaataagtca aagtaatcct gtctggtcct taatagtggt
    14641 actggatgtt tcattgtatt ttctcctctt tttactacca aatagctaat tccgactgtt
    14701 ctaatcgaag taagccttca ataaaaaaat aatcagaata gatcagtggc acattataag
    14761 tcagttctct atggtatgct tctgctccgc ctccaatgat cccatcttca tccatattcc
    14821 aatttgcata gtgcttttct gtcttttgga gtatattgat agcagcttca tgatacattt
    14881 ttgatcgtac agaggaaaca tatcctgcta attccagaag accgcatgca gcgcatactc
    14941 cagcactggt atcaatattc gaatctttta cagcaaaatc aatgagagga atataaccag
    15001 ttttttcgat tttctgaata aaatttactg ctatcttttc tgctatagct agatagtacg
    15061 gattttttgt gtaacgataa cttaacgcga atccatataa agcccatgca tttcctctgc
    15121 tccaacaaga attctcattg tatccttgcc cgccaagtaa ccgaatatat ttccctgatt
    15181 ctgcatcaaa ttctgcgata tgcccggttg atccatcttt cctcactaga tacttagctg
    15241 ttgtatctgc atgctgccga gcaattttag agaacctcgg atcaccagtt tccctcgaag
    15301 cccaatacag taatggtaaa ttcatcatac tatcaataat catccaacca ggtttatggt
    15361 cattccatgc tactaaatag ttccctgaaa tattatagcg tccagcaagt accgtagctg
    15421 catgtaaccc tagttgtcga ctatgtttgt tttgagactg ttgataatgt gcaatagccg
    15481 atggtaagaa taaaaaacct acatcatgat gaacgtctga aaaatggtac agggcagatt
    15541 ctaataattg ttcttgttgt attgctgcct ctttgaactt ttctcgtttt gtcttgtgaa
    15601 agagctgcca taaaatacca ctccaaaaac cattggtcca ccagtcgatt cctctgtcct
    15661 ttattacgtc tgtatatttt ccgttgtaag aaatatatgg aattttattc cccattctgt
    15721 ctaattccgc atctatcttt tcagaagcta cattcaaaat ttttcttgac cattccttcg
    15781 tctgttcatt catggatatc ccgccattct tttaattttt atgaatgtgg agttgctcct
    15841 agaacgaact agatggttaa cattcgttat tttttttact tatttgcagc tccaatgtca
    15901 ttctccttaa atttagactg cgcccacaca taaatcaaga ccattgggat cactgaagtt
    15961 gctaaaatta gcataattgc gccataatta attggctcac ctgtactaga aaccgtcata
    16021 tttttgataa ttaagggaac cgtatattta tctttatctg aaagcatgac taatggaaat
    16081 agatattgat tccaacttcc cataaagata agtaaaccac tagtaataat cgtaggtgat
    16141 aaaatcggta cagcgatttt cgtaaaaatc acccactcat tggcaccatc gattctggca
    16201 gcctccatca gtgctgtagg aaattgactg ctatattgtc gcatcataaa cactgaagtt
    16261 gtactggcaa tcgaagggat gatcacacct gcataagtat tcgataatcc taaacgactc
    16321 ataatgtaaa ataaaggaat caatgtggta aaaaaaggca ccatcctaga tagcataact
    16381 gtattgaaca acagtttatt ccccataaaa cgatactttg ctaatgcata acccgccatt
    16441 gtagaactgg ctgtagctaa aactgtactg attacagcaa taacaatcga attccaaata
    16501 atcaagggat aattatatct cgccatgatg gcttgccagt tttttaatag catatcccca
    16561 aacttgaaat tgattccgcc acttaaaatt tcttgattac tttgcgttga agctacaatc
    16621 atatataaat atggcatcaa aaatattaag gcaccaaaca caataatgac gtacgtaaaa
    16681 attctgcttt gtatcttttg tttagtcatt tttatcacct ccgaatttaa attgaatagc
    16741 cgataatata acagatacaa acaccatcgt ccaggcaata gcagaggctt ttccaaaatc
    16801 aaccatttca aaacttgttt tatataaatt gactcctacg accaaagctg aattttctgg
    16861 tccattggaa gtcccaaaga atatatttgg gatattgaaa ttacccaagc cattgatagt
    16921 ggctaaaact gtggccatta aaatgattgg ctttagcatg gggatcgtta ataaaaagaa
    16981 ttgccgaaat ttcgatgctc catctatttc agcactttcg tatatctctt tagaaatccc
    17041 ttgaagacca gctaaaaata aaatagtgtt atatcctaca ctaagccaaa tcatcgtgat
    17101 gatcattgat attcgtatcc aaaaaggatt tgttaaccac tgtacaccgt taattcctag
    17161 cgcattcaaa aatccattga ataacccagt cggattgaaa aaagtcatga atacggaagc
    17221 tacagccacc gttgatgtaa tggttggaat ataatagaca gttctaaaaa atccttttcc
    17281 ttttaactgt gctgaattca ataaaacagc aaagtaaata gctaatagca tcgttattgg
    17341 aattgatcct gccatgataa tcaaaacatt cgtgtaagat ttaataaaca atggatctcg
    17401 gaagatagct agataattgt caaatccaac aaattcaaat gagtagccaa ttttcataaa
    17461 actaaaagca aacgagataa tgatgggaat caaaatcgtc acaataaaga aaataacaaa
    17521 aggtgtgacc aatccataag cacttttttt atgagacgta tctgttgctt ttttcttgct
    17581 gttctttatt gtcttgcttg gtaacgaaga ttgcatcatc gttaagtcct cctaatattt
    17641 tttaatcacc agttaatttt acgccgtact tatgagcaaa attttctgct tcttttagat
    17701 atgaattttc aaaatcacct gtttgccaat agttatatgc agcggtagtc aaataagacg
    17761 tagcatctga ataagtggct ggtaaaacag tatttgatgt attttcattg attttattca
    17821 atagttcata gtatttttgt cctccttagt agtcaaactt tttctcagct gcttctgttt
    17881 gataggccaa tgagttagat gtagccacgc ccagctccag tgcgttggct aagcattctt
    17941 catctgtcag cataaatgta agaaattgtt gtgccacttg tggatttttg gcatctttag
    18001 ggacataaaa acttgatcct cctgatactg caccttttcc tggattttct tttgaaaaag
    18061 gaatcagtgg agccatgcgc catttgtcag atgcatcagg aaaattcaaa gacatattcg
    18121 tagcatacca gccgccagca acaaacattg cagattcttg aaatgctttc tcagcatctt
    18181 gtgcaccgta gaaatgtaca atatctgagt cgatcatttc tttgattaac tttgcagcat
    18241 gtttcgcttc tttagtattc agacggatat ttccttcttc atcaagcaaa gattgattct
    18301 gctgggctag catgttgata aacaattctt ggtccccaga ttctggcaag gcaattccaa
    18361 aactaccagt tttttctttt aatgcttttg ccacctggat atattcttcc caagaactga
    18421 tttcttcaaa atctacacct atttcagcaa atttttttgg ttgataataa caaagtgctg
    18481 gggtaaaatc actagcccaa ggaataatcg tttcattact cgtttgttga cgcaaaatat
    18541 tcaatttttg atcataaaac tgatcgccat atttgtcata aaatccatat tcatcagcac
    18601 tataaaaagc atcttgaaat ttttccagca cacctgctgc tgaagtgtct gccatggaga
    18661 taatatccgg catatcttcg tgtgttacca attttggggt gatcatttgt actgttgctg
    18721 ttcccgaagc tacattcaca atttttaagt caatatttgc tccagttttg cggttataga
    18781 tttttgcagc attttcaaca tagtttgtca gtttgccgtt tgcgacatat acttctaatg
    18841 tagtggcttt ttttatcatt ggatctgcga tgtttgctgt tacttcttcg ttagtttttc
    18901 cacatgcact taagctgaat aatagggtaa tgtttagcaa agctatcacc aatttgtatc
    18961 tttttttcat gtctatccct tctttctttt caagttctta tataaaacgc tttcacttat
    19021 gtatattaac ggaattagta acacaaatat ttatataaaa aaatatttta cttgtcg
//
LOCUS       DAZULY010000026        18797 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000026 DAZULY010000000
VERSION     DAZULY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.026"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..3393
                     /locus_tag="ACG81A_000638"
     CDS             4..3393
                     /locus_tag="ACG81A_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5014031.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            3396..4817
                     /locus_tag="ACG81A_000639"
     CDS             3396..4817
                     /locus_tag="ACG81A_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5014032.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            4814..6691
                     /locus_tag="ACG81A_000640"
     CDS             4814..6691
                     /locus_tag="ACG81A_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5014033.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            6783..7625
                     /locus_tag="ACG81A_000641"
     CDS             6783..7625
                     /locus_tag="ACG81A_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5014034.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(7777..8652)
                     /locus_tag="ACG81A_000642"
     CDS             complement(7777..8652)
                     /locus_tag="ACG81A_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5014035.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            8859..9890
                     /locus_tag="ACG81A_000643"
     CDS             8859..9890
                     /locus_tag="ACG81A_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5014036.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            9979..10284
                     /locus_tag="ACG81A_000644"
     CDS             9979..10284
                     /locus_tag="ACG81A_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFS5014037.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            10463..12478
                     /locus_tag="ACG81A_000645"
     CDS             10463..12478
                     /locus_tag="ACG81A_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5014038.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            12480..12764
                     /locus_tag="ACG81A_000646"
     CDS             12480..12764
                     /locus_tag="ACG81A_000646"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5014039.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            12790..14163
                     /locus_tag="ACG81A_000647"
     CDS             12790..14163
                     /locus_tag="ACG81A_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS5014040.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(14246..16261)
                     /locus_tag="ACG81A_000648"
     CDS             complement(14246..16261)
                     /locus_tag="ACG81A_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFS5014041.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16283..17497)
                     /locus_tag="ACG81A_000649"
     CDS             complement(16283..17497)
                     /locus_tag="ACG81A_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5014042.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            17717..18661
                     /locus_tag="ACG81A_000650"
     CDS             17717..18661
                     /locus_tag="ACG81A_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS5014043.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
BASE COUNT         6391 a         3502 c         3578 g         5326 t
ORIGIN      
        1 aaaatgacga ccacagggaa gaaactgaaa gttattttca tgctgataat attgagttta
       61 tcaaactttg tgccattatc tgcaatagca gacactacag atgatccaac agttttagaa
      121 acaatttcag ctgaagtcat ttcggatcag tctggaaaaa aagcactgaa catcaagcta
      181 aatgcgaata acaccagtgc tgaaaagata gaaaaagaaa ttggtctagt cgaaaattac
      241 ttaagtgatg tggaaagaaa agaaggagat ggctatgctt atcaggtaaa tagcgggaaa
      301 attacgttgg aaatctcatc aaacactaaa caaactatcg atctgagttt tccaatcgat
      361 ccagcacttt accacagcca ggcaaacaag ctgatcgtcg ataataaaga atatgacatt
      421 attgatgaga cagaaaataa gaaagataca gatgtgtcag taccaaagcc agacgaaata
      481 gaagaagaat catcaaaaga aaacgaaaat tctgtcagcc catttacatt gcctacatta
      541 tccttgccag ctgtgagtgt gccatctaat caaacgattc ctacagaata tacaacagat
      601 gatcagggca cttatcctaa agccagttgg caacctacag gaaatacaaa tgttcttgat
      661 catcaaggca ataaaaacgg aacaaatcaa tgggatggta taaattcttg gaatggagat
      721 cctaatgatc ggacccattc gtatatcgaa tatggaggaa ccggtaatca agcagactat
      781 gcgatacgaa agtatgcaaa ggaaacaagt acacccggat tgtttgatgt ttatttgaat
      841 gctcgtggaa atgtacaaaa agatatcacg cctcttgatc tcgtattggt cgtagactgg
      901 tcaggaagta tgaacgacaa taatcggatc ggtgaagtaa agattggtgt cgatcgtttt
      961 gtcgatactt tagcagatag cggtatcaca gacaaaatca atatgggata tgtcggctac
     1021 tcaagcgaag gatatagcta cagtaacggt gcagtacaga tgggttcatt tgattcagtg
     1081 aaaaatcaag taaaatccat tacaccttca cggacaaatg gtggtacttt tacacaaaaa
     1141 gcactaagag atgcaggaag catgctatcc gttccaaatg gacataaaaa agtgatcgtt
     1201 ttgctgacgg atggtgtacc aacattttcc tataaagtac agcgggtaca cgcacaatca
     1261 agcagcaatt attacggaac tcagttttct aatacgcaag atcggccggg aaatacttct
     1321 ctaatctcaa gaatctatga tgcacctgac caaaacaatc tatccagaag aatcgacagt
     1381 acgtttatcg caaccatcgg agaagcgatg gcactcaaag aacgaggaat cgaaatacat
     1441 ggtcttggca tccaacttca aagcgatccg gcagctggtc tctcaaaagc agaagtagag
     1501 tctcgtatgc gacaaatggt ttcatcagat gaaaaaggcg atctttacta tgaatcagct
     1561 gatcatgcaa cagatatctc tgaataccta gccaaaaaag ctgtacagat ctcagcaact
     1621 gtaagcaatg gacaaataaa tgatccaatc gcagaaccat tcatttatca gcctggtaca
     1681 ctttcagtca agagtgtggg gacaagtcct acaacggtca ctccatctat ttccatagaa
     1741 ggaaatacca tcaagagcaa tcagatctat ttaggaaaag accaagaaat ccaaatccat
     1801 taccaagtga gaatccaaac agaaaatgag gacttccatc caaatttctg gtatcaaatg
     1861 aacggcagga caactttcca gccaaacatt gataccaatg aattagctga attcggtata
     1921 ccatctgcta aagctcccgg agtcagtctt cacatcaaaa agttatggga agaatttgac
     1981 aacaatctag ctgatcgtcc agatcaagtt acttttgaga ttcaacggga acatacgaca
     2041 aatgctgcag cttggaaaaa cggatatatt cgaatcatta aaccagctaa agatacaaca
     2101 aatacgtggg aacgtgcaga cattgacaaa ttatctgcaa atagcggaga aagttatcaa
     2161 gagatattat cactacctca atacaataat caaggtcaag cattcagtta ccaaacaatc
     2221 aaagaattac ctgtaccagg atacgattct caacaaatag atgcaatgac atggaaaaat
     2281 actaaacaat tcacaccgtt aaacttgaaa ataacgaaaa attcctctac aggtgaaaag
     2341 gatcttattg gcgctgtttt caaattaaca ggagattcta ttgatacttt actaacagat
     2401 catggcgacg gaacctattc tcttccagaa aatgtcaaat tgcaaaaaga aatgacctat
     2461 acgctgacag aaacaaaagc tccagaaggg catggattaa gcaaaaagac tacttgggaa
     2521 atcaagatcg cttctgatgg tacggtaacc attgatggaa aaacagtcac tacttccgat
     2581 gatacgatcc agttgactat tgaaaatcct tttgttgaag ttcctgtagc agtacgtaag
     2641 tatgcgatgc aagggacgga caaagagata aatcttaaag gagcagcatt ttccctacag
     2701 aaaaaagaag caaatggtac ttatcagcca attgacagcc aaacaacgaa tgaaaaaggt
     2761 cttgccagtt ttgattcact cacacctggt aaatatcgag tcgttgaaac agctggtcct
     2821 gccggatatg atacttcgcc gggaaattat gaattccaaa tcgataaata tggaaaaatc
     2881 atttacacgg gaaaaaatac cgagatgaca aataatgtat ggacgctcac tcatcaaaat
     2941 cgactaaaag cgtttgatct aacggtacac aaaaaagaag acaacggaca gacattaaaa
     3001 ggagcaaaat tcagactgca gggaccagaa atggacttag aatcgccaaa agatggacaa
     3061 gaaacagata cctttctatt cgaaaattta aaacctggaa cttatacgct gaccgaaact
     3121 tttacaccag aaggatacca aggtctaaaa gagccagtta ctatagttat acacgaagat
     3181 gggtcaattc aagtggatgg acaagatcat gaatctgttc tgtcaccagg agccaaaaac
     3241 aaccagattt ctttagacat cacgaatcag gcaaaagtac cattacctga aacgggagga
     3301 attggccgtt taggaatcta tctagtaggg atgattggtt gtgcgttttc tatttggtat
     3361 ctttttttga aaaaagaaag agggggcagc taaatatgaa aaaacttggt tggcttagta
     3421 tgtgtctctt cttgttacta tttaaaccag cttttactca ggtagcaaca gaaacagaaa
     3481 cagaaatggt tcagattact ttacacaaat tgcttttccc aaacgggcaa ctgccgaaaa
     3541 atcatccaaa tgacggacaa gaaaaagctt tattacaaac gtatcgagga ttaaatggtg
     3601 tcacattcca agtttatgat gtcacagatt ctttttacca tctacgggaa aagggcaaaa
     3661 cggtagaaga agcacaagca gagatcgcaa aaaacggtgc gtcttccggt atgtttaccg
     3721 cagaagcaac aactacaact cttaacaacg aagatggtat cgcttctttt tctctggccg
     3781 ctaaagatca agaaaaaaga gataaagcgt atcttttcat tgaatccaaa gtaccagaag
     3841 tcgtcaaaga aaaggcagag aatatggtag ttgttcttcc tgtacatgga caaaacaatc
     3901 aaaaactttc aactatccat ttgtatccta aaaatgaaga aaacgactac cctgatccac
     3961 cttttgagaa ggtattagaa gagcctagaa atgattttac gattggtgaa aaaatcactt
     4021 attccttgca tacgacaatt cctgtaaata tccttgacta tcaaaagttc gaattgtcag
     4081 atagtgcgga tgaagcatta acgtttttac ctaatagttt aacgatttca tcgaatggag
     4141 aaaagctgac agaaggcttt gtcatacaca agaaacctca cggatttgat gttttatttt
     4201 cgatcccttc gttggaaaaa tatgctggaa aaaaactgac catttcttat cagatgcagc
     4261 taagcagtac agcacaggcg aacaaggaaa tcaacaacaa cggaacactg gattttggtt
     4321 ttggtgtcag tacaaagaaa gtctctgtat atacagggag taagcaattt gtcaaaatcg
     4381 agacaaataa accagataaa cgattagctg gcgcagtatt ccttattaaa aacaaagcag
     4441 gaaattacct ccagcaaaca gccaacggat acaagtggac aaagaacgaa tcagatgcgc
     4501 ttcacctgat ttccgataaa aatggcgctt tttcaatttc cgggttgaaa acaggaagtt
     4561 atcgattaaa agagatcgaa gcaccttctg gttatatttt aagtgaaaca gaaattccgt
     4621 ttaccatttc aacttttctt tctgaggata aagaggcgga cagtatattg aaagtagtca
     4681 ataaaaaaga aaatagccgt ccatttcttc caaaaacaaa cgaaacgaaa aatacacttt
     4741 taggcgttgt tggtatggta ttcgcaagct ttgcaatctg gttgtttatc aaaaaaagaa
     4801 caggagtgaa aaaatgaaaa atcataaaaa aataaacgtt atgttaggag tccttttcct
     4861 tattttacca ttactcacaa acagcttcgg cgcaaaaaaa gtgtttgcag aggagacagc
     4921 agctcaagtc atccttcata aaaagaaaat gactgattta cccgatcctt taatccaaaa
     4981 cagcgggaaa gaaatgagcg aattcgatca ataccaagga ttagccgata tttcattttc
     5041 agtttataac gtcactcaag aattttatgc gcaacgagat aaaggagcgt ccgtggatgc
     5101 agcaaaacaa gcagtccagt ctttgactcc tggtacacca gttgcttcag gaacgacaga
     5161 tgctgatgga aatgtcactt tatctttacc taaaaaacaa aatgggaaag atgcagtcta
     5221 cacgatcaaa gaagaaccaa aagacggagt gtcagctgcc gcaaacatgg ttttagcttt
     5281 ccctgtatat gagatgatca aacaagcaga tggctcttat aaatacggga cagaagaact
     5341 agatactatc catctctacc ctaaaaatac agtcggtaat gatggaacgt tgaaagttac
     5401 aaaaatcggt actgccgaaa acgaagcact aaatggagca gaatttatta tttctaaaga
     5461 agaaggaaca ccaagcgtca aaaaatacat ccaaagtgtc acagatggat tgtacacttg
     5521 gacaactgat caaaccaaag ccaaacattt cattactggt cattcttatg acatcggcaa
     5581 caatgacttt gccgaggcat ctattgaaaa aggccagttg atcgttaatc atttagaagt
     5641 tggaaaatat aatttagaag aagtaaaagc tcctgataat gcggaaatga ttgaaaagca
     5701 aacaatcacg ccttttgaga tcctggcaaa tagccaaaca ccagtagaaa agaccatcaa
     5761 aaatgatacg tctaaagttg ataaaacaac acctcaattg aatggaaaag atgtcgcaat
     5821 cggtgaaaaa attcaatatg agatttctgt caatatccca ttaggtatcg ctgataaaga
     5881 aggaacgcaa aacaagtaca caacattcaa acttatcgat actcatgacg ctgctttaac
     5941 atttgataat gattcttcag gaacgtatgc ttatgcctta tatgatggaa ataaagaaat
     6001 cgacccagta aattattctg tcactgagca aacagacgga ttcacggttt cagttgatcc
     6061 gaattatatt ccttcattaa ctcctggcgg tacattgaaa ttcgtttact atatgcattt
     6121 gaacgaaaaa gcagatccaa ccaaaggatt ttctaaccaa gcaaatgtcg ataacgggca
     6181 tacaaatgat caaacaccac cgtcagtcga tgtcgttact gggggcaaac gatttgttaa
     6241 agtagatggt gacgttacat cagaccaaac acttgctgga gcagaattcg tcgttcgtga
     6301 tcaagatagt gacacagcga aatatttatc gatcgaccca tccacaaaag ccgtcagctg
     6361 ggtatcggcg aaagaatcag caacggtttt tacaaccaca agtaacggtt taatcgatgt
     6421 gacaggtcta aaatatggca cgtactatct ggaagaaacg aaagcgccag aaaaatatgt
     6481 tccattaaca aaccgtgtag catttactat cgatgaacaa tcttatgtaa cagcaggaca
     6541 gttgatttct cctgaaaaaa taccaaataa acacaaaggt acacttcctt caacaggcgg
     6601 taagggaatc tatgtgtata tcggtgcagg agtagtcctt ctactgattg ctggactgta
     6661 ctttgctaga cgcaagcaca gtcagattta gtctttttgc aagaattgga aaaaggagca
     6721 gcggattacg caaatgctcc tttttctttt attatcaaac agtcatcaca aggagaacac
     6781 aaatgaaacc aaagaaaagg aaacttctag atctgctgat gcttttcttg cttctctcag
     6841 gaatagggat actagcttat ccatttgtta gcgacgcgct gaataattat ctggatcagc
     6901 agatcatctc tcattatcag caacaagcgg tcaaagaaaa tgaagaagtc atggctaaaa
     6961 tccaagaaaa catgacaaag aaaaaccagc aattagcaaa agaaggtgga aatccaggtg
     7021 cagatccctt tactaagaaa aaagaacctg cgaaaaagga tagaacctat tttgagaaac
     7081 atacgatcgg tattttgacg atccctgaga tcaatgtcca tttgccaatt tttgatgaaa
     7141 cgaatactgt gctgctagaa aaaggtgcct cattattgga aggaacgtct tatcctacgg
     7201 ggggagaaaa tacacatgcc gttatttcca gccatcgagg actgcctcaa gcaaagttgt
     7261 tcacagactt accgaaactg actttaggtg attctttcta tatcgaaatc aatggacgga
     7321 cactcgctta tcaagtcgat cagatcaaga cggtagaacc gactgataca aaagatctga
     7381 gaatcgaaaa agggcaggat ttcgtgacac tcctaacttg tacaccttat atggtcaaca
     7441 gtcatcgctt acttgtcaga gggcaccggg ttccttatca tgcaaaagaa gttcaaaaag
     7501 aagtacagaa agtatccgag aacaaacgat ttttcctgta tggattgtta gctgcatgta
     7561 tactgatttt cctccttctt atatatcttt ttcgtcaatc gttcaaaata agaaacagaa
     7621 gttagctatt cctcatttct ataatatatc tattcgaatc gttccgtaaa caaacaggct
     7681 gaggtttgaa aaagaaaact tcagcctgtt tttatgatga tatcaaagag agcctatcaa
     7741 acactatctt tcttattact aaatgaggat cttttcttaa gttttccgat attctttaag
     7801 aaggcctttt tgcatcaatt cttgtttgtt tttcaagttg cggatattct cttcaaaatt
     7861 ttgagaatat atcactgaga ataacgtatt taatacgtaa tcgaaggcaa tttgagagga
     7921 gaaagtcgct actttagcaa aatcatattc ctcctgcgat ataacaagac acatatcagc
     7981 ctgtttggcc atttctgaat gctgatttcc ggtaatcagc aaggtagggg cctctacatg
     8041 cttcaaatag gtcattattc gttcataatg atggatgcga ccgctgtagc taataaaaat
     8101 ggcgcaatcc tcgctagtca aattggctgc attccaagaa gaatcactgt attcttcagc
     8161 catgatcaaa aacttattca acttgaccat tttattctgg aatttccttg ccgtgatttg
     8221 agaatcacct tttgcaaaaa gaaagattcg ctgtgccttg ttcaatactt taccgatttg
     8281 atctaaatct tcatgttcaa tctgcaattg tgcttttttt attgactgga tcgataaatc
     8341 cgccattttc tttgcaattt gctgagtgct atcttcactt gaaaaaggaa aattaggatc
     8401 aatcgtttga ttgaactgag caatcaattg atgaacttcc cgccccagct cgaaacggaa
     8461 ttctttaaat ccgctgaatc ccattttttt gcataaacga atgatagctg aatgggaagt
     8521 atacgtagct tttgctaatt cttcaatcgt catgtagact gcagcttcga tattcctacg
     8581 gatgtaatcg acaatccgtt tctctgtcgt ggtaaacaac tcttgttttg ttagtttttc
     8641 ttcaataagc atgatctgac ctccaaattc tatcttactg ataaacactt ggttccgcta
     8701 ctatcttttt gatttttatc cttactttga acattaattt caccaaaccg aatgataaca
     8761 agtgtttttc tgaacttttt taccagaata gctattataa atactttctg cgcttttcta
     8821 ggatatactg aaaacatcaa taaaaggagg caccactcat gcttacaatc gcttatcttg
     8881 gcaatggaaa gagtacaaat cgctatcatt tgcctttttc aagaaaactt acgaacaaaa
     8941 tcaatatcaa aaaaattttt tccccatctg aaccagtgtg gaaaacattc gaagacatcg
     9001 aatatactga tcagctgtct gatctttggg atgatcctga gatccaatta gttgtcatca
     9061 ctacacctag tcaatttcat tatgaatatg gaaaaatggc actcgaacat ggtaagaatg
     9121 tactagtaga gaaacctttt gcagaaacgt caaaggaagc agaagagtta tttgcattag
     9181 caaaagaaaa aggtctattt atccaatgtt accaaaacag acggttcgat tctgacttct
     9241 tgacagtcca aaaagtaatc gaaagcggaa aattagggga tattttagaa gtagaaatgc
     9301 attatgatta tttcagaccg gaagtaccag aaaatacgca tgaattttct actgcgaaca
     9361 gctatttata tggacatgct tgtcatacag tagatcaagt tctttcttat ttcggcgaac
     9421 cagatgatat cagctatgat gtacgtcaac ttttagggaa aggacgaatg aatgattact
     9481 tcgatctgga ttttttctat ggaaatatga aaatttcgat aaaatccagt tatttccgca
     9541 tcaaagaacg tccaagtttc gtcgtttacg gaaaaaaagg gatgttcgtg aaacaaacaa
     9601 aagatcgaca agaagaacat ctgaaaatgt tttacatgcc tgatcatgaa gactttggta
     9661 tcgatttgcc cgaacattat ggtgtattga cctatataga tgaacaaggg agttatcatg
     9721 aagaaaaagt aatttccgag gttggtgatt acagccgtgt ctacgaaggg atttacgaga
     9781 cattagttaa tggcgcagaa aaagtcatca aagatgaaga aacaatctta caaatgaaaa
     9841 ttttggaaga aggaatcaaa agcattcaag aatcagaagg aaaaatatga catatttgta
     9901 agaatcgggt ttgttttttg acgcataaac tgtcttattg tacgatgaaa aagtatagaa
     9961 ctactaggag gaaccaacat gacaaattat gacggtgaaa aacacaaacg attaagaggt
    10021 tcaatggccc ctaaaacaga agaagaagtg gctttagcag gaactaattc tggtgaaaca
    10081 gaagacaaag aagaatcatt agctggcaca aactctggtg aaacagaaaa taaagaaaca
    10141 gctttatctg gttcaaattc tgtagaagag gaaacaaagc ctaatgagcc caatcacttt
    10201 gaaaaagata tcgaaaaagc ggttgaagat gtcaaagaaa aatttgaaga actgacctca
    10261 aaaaataaac atgatcatca ttgatcggac gaaatcaatt cataaaagca ggagacattt
    10321 ggtcacctgc tttttttctg tatccattcg atagaaatcg ttcggtacag tcgtaaaaaa
    10381 aatccaactg taagttgtac caaacacccc tcgatttaaa agcgctacca tactagaata
    10441 taagtaggag gtgaacagcg ctgtgaacta caaaattagt tatctcctga aaacaccaag
    10501 agaaatcagt ctagaaaaag cagcagagat catcgaagta aatacaagcg aggcgatcaa
    10561 acaattaact ccttttgccg aatatgtgga tgatacccgt atccgtataa agaaattccg
    10621 agcaatcgac tgggcagaaa aatttttgga tattactcga tttgaagctg tgtataccga
    10681 acaagagcga agagatcttc tttatttgat ggtttattcg actgcaacgt ctgtttctgt
    10741 ttttcatttt caagattttc ttcaggtttc taaagggaca gtcctgtcag atatcaaaaa
    10801 gataagaaat gaattagaag tggaagattg tcagctgtcg tatactagga aaactggttt
    10861 taggctgcta gggtcagaag caatattaag aagagaagca aaaaatcgca ctgctcgttt
    10921 gatgcagtca cttgctggaa gatttgggct cacgtattta accgcaaccc tttccattga
    10981 ttattacgcc aaagttagag atttgctaga taaaacgatt tcactatcag atcttcgatt
    11041 cgcaccaaat cgaatggatg aattagctta ttatttgagt ttgacaacca ttcgaattgg
    11101 aaaaacatcc atggaactgg taaaagatgc tgaactgttg gcttctcttg cagtcactga
    11161 cattagtcga caagtattga ctggccttgg ctattccgaa catcaaaatt cggagattat
    11221 atttttctct ctttgcttag catcgatcgt tgaaggagat gtccaggagc ctgcacttga
    11281 ttttttgctg gaatgttctt cagaagtcat ccatcgcatg gaattcctga tggccgtaga
    11341 atttgcttcg tttcgtgaat tattgatgaa tgtcttttat cacttggtcc ctgcctattt
    11401 tcgtatttcc tattcttttt atttacctaa tgtgatgatc gatcaaatta agcatcaata
    11461 tgcttccatc tacgaaatga caagaaaagc attacttccg ttagaaagac gaatagggaa
    11521 gagcatccct gaagaagaga ttggtttttt cactatacta tttggcggag agatcagaaa
    11581 agcggacgca gaagaaagaa atagaaaaat aagagccgtg atcgtctgtc caagtggcat
    11641 tagctcttca ttgatcttaa aaagtgaact tcagcaatta ttcccgatga tcttttttac
    11701 agagactaat tcagctgacc gcttacacga ggttgacgaa acttcttacg atattatttt
    11761 ttctactgta cctctcacac ttactgataa gaaaaagtct ctctatgttc tgcatccaat
    11821 catgactgac ttggaaaaaa atcagctgat caatcaagta caaagagatt ggctgatacc
    11881 aggattttca atgccttctg cccaagaatt gttgtcggcg ctactgcctt atatcgaatt
    11941 aaaagaaggc gtagacgaag aaaagctcta tcaagtatta aatcgtaaaa tgaacaaact
    12001 tttagaaaag aaggaggatc aacgtcctat gctatctgaa ttattaacag aagaaatgat
    12061 ccaattatcg gatcaaccaa aaaactggca agaagcaatc acatttgccg cacagccgct
    12121 attatcagaa aataaaatag aagaaagtta tatcaaagct atgatcgagc gcgtggagca
    12181 gtatggtgcg tttatccata ttggcgatca catcgcatta cctcatgctc gtccagaaga
    12241 tggcgtaaac gaagtaggga tgtctttgct gaaacttcaa caacctgtgg atctgcttga
    12301 taatccagcc catcccatta gtttgttcat ctgtttagct gctatcgata atgaggcaca
    12361 tcttcgggct ttggcaaatt taacaaagct actttctaat aaacaaaatc ttcaagattt
    12421 attacacgca tcaacaaaag aagaaatatt agcgatcata agaaaaggag acgaatgata
    12481 tgaaaatttt tgcagtatgt caaagtggat taggaacaag ttttatggta gagatgaaca
    12541 tcaaacaagc acttgcagat cttggagcag atgtggacga gtttgaagta agtcatacgg
    12601 atgttggaag tgtatctagc gatatggctg attatttctt cattgagcat tctttgaaag
    12661 atacgctaac ttctatacct gatgaaaagc tcgtaccttt gcaatcgatc attgacagcg
    12721 aagaagtaaa agaaaaagta acgaagatct tgtcaaaaga ataaaaatga ttcaagggag
    12781 gaactatcaa tggataaatt gttgaatttg tttgtgtcaa tcgtcagtca gccctccatc
    12841 ctcgtttctt tgattgcact gcttggtctc gttcttcaga agaagaaatt ttcagaagtg
    12901 atccaaggaa ctatcaaaac atttgtcgga ttcctagtac tgatcggcgg tgctggttta
    12961 atcagtaatt cactaacccc ttttgcttcg atgttccaaa cagctctgca tactcaagga
    13021 gtagttccta gcaatgaagc agtagtggcg attgctttgc aacaatacgg tacacctact
    13081 gctttgatca tgctagtagg gatgatcgtg aatattttac ttgctagatt cacaagatat
    13141 aaattcattt ttcttacagg tcaagcaatg ctttatgttt cttgtctaac agcagtcatt
    13201 ttagtaagcg cggggtttac tgcaaatttg tggatgatct tattaggcgg ggtcttcgaa
    13261 ggaacactat taactattac gcctgctctt tgtcaaccgt ttatgaagaa catcactgga
    13321 aacaatcaag ttgcgttagg gcataccgga aatatcggtt atgcttctag tggatggatc
    13381 ggaaagtttt ttggaaacaa agaccgttct acagaggaca taaagattcc taaaagtttc
    13441 agctttttgc gagattctac tgtatcgatc atgctcttga tgtcgatcgt atatgtcgca
    13501 cttgctttgt tagctggtcc tggatttgtt gaaagagaac taagcaatgg agacaacgct
    13561 gtaatctttg cactgatcca agctggaaca ttcacagctg ggtttgttat cgtccttcaa
    13621 ggggttcgaa tggtgttggc agaaatcgtt ccagcattcc aagggattgc taaaaaactc
    13681 gtacccgatt ctaaaccagc attagatgtt cctatcgttt ttccatatgc acctaatgct
    13741 gtattaattg gcttttttgt tagcttcttt gtaggaacac tatccatgtt tgctatgata
    13801 ggcttgcaaa ctacagttat cattcctggg gtagtaggac atttcttctg cggagctgct
    13861 tctggtgtct tcggcaactc gactggcggt agaagagggg cggtattagg ttctgctttc
    13921 aatagcttat tgattagttg gctgccatta tttatccttc cagtattagg agatcttaaa
    13981 atcgcttctt ctacatttgc cgatactgat tatctagtac caggacttgt acttgggaaa
    14041 ctaggagaat acggtcaaat agccttgaca gttggaatca tcgctttctt tttacttgtc
    14101 gtattatcta gctttctttt tgccggaaaa agaacaaaca ttgtttctaa agaacagaat
    14161 taagcaaact tcaaaataaa aattgcttgg acaatatcag aagaagattt aaagtcttct
    14221 gatatgtcgc aagcagtttt ttgttttatg catttatttt tgaatagctt gttaagacaa
    14281 gtggctgttt tgctagaaga ggataacttt taccattcat tttcactgtt ccgcctgttc
    14341 tatcagaagt aatcacgatc ttatcttttg ttttttcaag cgaaatcttt ccatctttga
    14401 taggtagcgt acaattcagt gtttcaaatg aatctagatt aggttgtatc ataaattctt
    14461 cataacctgg ctttgttgga tacaaaccga taaaatagcg tcctaaaaga tagattgggc
    14521 ttgctcccca tgcatggcat aagctttttc cataaggatc tccatacata ctgtactttt
    14581 catttcctgt ttgagtaggg tcgtattctt cccagaaagt taccgcatca tgagataaca
    14641 tccctcccca ataatcaaga atcgtttggt agacgatatt cgtctgtccg agtttgcata
    14701 aagcatcttg ttcatagaat ttgaaatatg gagtagtaat ctgtgtgata gtgtcattca
    14761 ataagacatt ctttaagatc gattgctgtt ggtctttgtt agcaaaatcg aataaaacag
    14821 cgaaaatatt cgcatgacgc gtcacatttc ttctcccaga ttcatagcaa tcgataaaag
    14881 cgccaagttc ttcatcccaa aagtagtcaa ataggttttg tttcaatagt tgatattttt
    14941 cttgatagag atgagtatga taattttcgt tctctccata tgtttcagct aagatatctc
    15001 cgcaagtgat catggaatga taggctttca gtaataagac ttgttctgca gcaagcgttc
    15061 cttctttatc catttcagac caatcaataa atatccaatc ttttgctctc ccataaataa
    15121 accctaattc attcgtttgc tccatcaagt atctcattaa tgattgcatc ttagggtaga
    15181 cgatgcgtaa aaattcatag tctttcgtca tcatatagtg gttttcgata ccaatcaacc
    15241 acaagatcga atagtcaacg atagtattca tatgctgctt gatttcattt tgtcctctta
    15301 atgctaatat cgttcgttta ttgatttcct cgtcaaaaaa aagatattga ttgatcatga
    15361 atgattgata cgcatctcca gaccaaatcc agcgatctcg tttgataccg tcgataaaaa
    15421 acaaatcact acataacttg aaggcttctt ctgatactga ccagatctga ttcagtaaag
    15481 aagaatcgct tgaaaaagca gcttgtttcg aaagagataa aaattcatga aaaacttgaa
    15541 gttgaaccat tccagtagaa acctctggta taaacaaata gcgaaaagcg cgtttacgag
    15601 ctttcatttt tcttgtcatt cctatttgct tatagtagca ataatcgata tccatagctt
    15661 ctacatcgga ctctccataa catagagtga tagattcgat atggtcatcc agagatatta
    15721 gttctacaca gccattcact gctcgcccaa aatcgaataa gattcctccg ccaatctccg
    15781 tttcagtgac tggatggacg cattcttttt gataatagat ttgatttgga tcttttgcga
    15841 tatcgacata aagcggactc cagccagctg gatattcttc aataaaatta cttgctgtcc
    15901 agccaatatc tgattttact tcgtctccct cgataaacat cgctggtagc cctgatgtat
    15961 gtcctacaaa gatacgaatt tttgctttac cagctggaca agtcagccat tttccacatg
    16021 gatgtttttg tccattaatt tctacatacc caactccttg gatcgtcact ttaaacatgg
    16081 aaggttgatc aagaaagtaa tatctcttaa acttgacgtt ccgatggcaa tcatccatgt
    16141 accagtacgc tggccagtca aaacctcgtt cttcccgttg gaaattttgt agcagtccat
    16201 ggcggatctc aaagtctcca ggataccaaa gccatctatc tttatgtttt tcatttgtca
    16261 ttttacacga cctccatcaa atttattttg ttgtcaattg ttcttttatt tcttcatcta
    16321 ctcttatcac tgtctttcta ttcgtcctgt gtactactag aaggctaata agcgcgctaa
    16381 tcaacagcaa gatactgcca ttgacgaaaa cagcaagtgt tttactccca agagaagcaa
    16441 aagagagaaa gagcaaacca tatggtgcaa tgaaagtacc taaattagcg ccaaccaata
    16501 agattgaagt attgaaactc tccgcgtact tgggcgcttg gatacttact tggttgaata
    16561 agtaaggaat gaataacgaa aaagaaattc ctgttaggat cgcaccaaac ccaatcacat
    16621 acaggtttat cacagtggca atcaatacaa agccgattcc catcagtagt aaagaaagtg
    16681 taagcatata gcgatgtgct tttgtctgaa gccagccaaa acaaactccg gcagccatac
    16741 ttgccaattg catgattgtt aagatattcg ctgcattagt ttcgtttcct agagaataag
    16801 tcgtcaggac tgtggctaat ttaatcgata cagcattata taccacgatg accagcaaga
    16861 gaaaaatacc ataactaatc attttaggat tcgttttgat tacttgtctt gttggggctg
    16921 tgccatgcga ttgaagttca gttgtttcag gtgtaggaac aaacaaaaca aacaagacaa
    16981 gaataggtaa gctgatggcg tagactaaaa aggtatattg ccaaccgaac tgtactagct
    17041 gtcctgcaac aaatgtcatt aaagctgcac caaatccttg aaatgcactt tgaaaaccaa
    17101 tcatagaggc tcgttcatct ccgtgataaa aggcagcaat catgctgact gccaaagaat
    17161 tgatcaatcc aaagccaata cctagtccag ctcttgaagc taagacaata ctgtaacttt
    17221 gtgagaagag ggggaggata ccacaaatcg atgcaatcac taatccgatg atcacagttt
    17281 gtttttgtcc gattttccta gcaataaaac tactaatcaa aacaaaaata gtgaccataa
    17341 aagagggagc agtcgttaat aattcaacag cagataaaga acgcgtggca taagtagctg
    17401 acatactggg aattgcagac gtgactgcac cagcagaagt caaaataaga gagactgaca
    17461 gcagagaaaa tttcatcatc tttttattca taagcatgtt cctttcattt ttgtaagcgc
    17521 ttatcaaaaa tatagtatga agtggttatt ctctcaatag cggaatcaca ggaagttcat
    17581 gtcccaacca tagaaagtct ttatgaaacc aaaaatagct ttgcatataa tagcagtaca
    17641 gcgtgaacta ataagcgaaa gaaaccgctt acaaaaagga gggagtcaat tggaactagc
    17701 tgaattagac atttttttgc gtaacgaaga atcaatcgaa actctgcagc gtgaacaagg
    17761 aatgaatatc aatgatttaa acttagctta ccatcttcag acaccgccaa aaatccaccg
    17821 aggggatcta tttcaacagg gaaatattca tatcagtaaa caccgtcgat atgcggaagt
    17881 tcccgagcat acacatgatt ttgttgaaat caattatatg tatagcggta catgtttaca
    17941 aattattaat ggagaaagga tagagatgga agaaagtgac gtggtgatca ttgataaaga
    18001 agctactcaa agaatcgaat acacagggca agaggatatt ttaatcaaca tcttattaaa
    18061 agaagaaacc ttatctacag agatattgaa tcatttggca agcacaaata atctactttc
    18121 ttcttttttg ataaatgcat caaaagaaaa aggggaacat acacaattca ttcattttcg
    18181 cggcggaaaa acaagtcgtt tgacgcattt gattgaaaat atgctgttga tttattttga
    18241 tacatacagt cataagatcc gttcgttaaa tctctataca tcactattac tgatcgaatt
    18301 gactcattta ttagaacaac aaagcttttc atcagcagat ggatgcgcag aaaatgagat
    18361 gcttagagta ttagattata tcgataagca ttttacttct tgtacgttaa aagaaacagc
    18421 agctcatttt ggctacaatc cggtctatct tagtaataaa ttgaaaatgt attttgatgc
    18481 ttcctttcaa gaactggttc taaagaaaaa attcgacttt gcgttggaac tgattcaaga
    18541 aacgagtttt tcgttggaag aaatcgctaa tcgtatcgga tataaacaaa cgacttcttt
    18601 attcaaatta ttcaagaagt attcaacatg tactcccaat gaatggagaa ataaacatta
    18661 agaagaaaat ctttcactaa agcatatgaa ggaaaattgt tttaggtgcg attgctgtct
    18721 tattatatcg ttgcagcgaa tttttttgga tttattttca gttctaaact tttggttgta
    18781 ttatcagggg atatagg
//
LOCUS       DAZULY010000027        18694 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000027 DAZULY010000000
VERSION     DAZULY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.027"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1114
                     /locus_tag="ACG81A_000651"
     CDS             <1..1114
                     /locus_tag="ACG81A_000651"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFS5014044.1"
                     /translation="RFFLTILLSLILTPVFKIISVQTGMVDKPNERRINKVPMPSAGG
                     LPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPKQKSIGILIA
                     SLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDGLDGLAAGIS
                     LIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLGDTGALFLGF
                     MMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTADKKHLHHRL
                     ISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAILLFELIGLIN
                     EKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            1107..1907
                     /locus_tag="ACG81A_000652"
     CDS             1107..1907
                     /locus_tag="ACG81A_000652"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5014045.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            1907..2734
                     /locus_tag="ACG81A_000653"
     CDS             1907..2734
                     /locus_tag="ACG81A_000653"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5014046.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            2739..3446
                     /locus_tag="ACG81A_000654"
     CDS             2739..3446
                     /locus_tag="ACG81A_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5014047.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            3546..4412
                     /gene="rfbA"
                     /locus_tag="ACG81A_000655"
     CDS             3546..4412
                     /gene="rfbA"
                     /locus_tag="ACG81A_000655"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFS5014048.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            4426..4998
                     /gene="rfbC"
                     /locus_tag="ACG81A_000656"
     CDS             4426..4998
                     /gene="rfbC"
                     /locus_tag="ACG81A_000656"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFS5014049.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            5020..6048
                     /gene="rfbB"
                     /locus_tag="ACG81A_000657"
     CDS             5020..6048
                     /gene="rfbB"
                     /locus_tag="ACG81A_000657"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFS5014050.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            6146..6997
                     /gene="rfbD"
                     /locus_tag="ACG81A_000658"
     CDS             6146..6997
                     /gene="rfbD"
                     /locus_tag="ACG81A_000658"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFS5014051.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            7032..9065
                     /locus_tag="ACG81A_000659"
     CDS             7032..9065
                     /locus_tag="ACG81A_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014052.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            9109..10389
                     /locus_tag="ACG81A_000660"
     CDS             9109..10389
                     /locus_tag="ACG81A_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFS5014053.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            10599..11405
                     /locus_tag="ACG81A_000661"
     CDS             10599..11405
                     /locus_tag="ACG81A_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5014054.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            11417..12637
                     /locus_tag="ACG81A_000662"
     CDS             11417..12637
                     /locus_tag="ACG81A_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5014055.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            12627..14213
                     /locus_tag="ACG81A_000663"
     CDS             12627..14213
                     /locus_tag="ACG81A_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5014056.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            14252..16390
                     /locus_tag="ACG81A_000664"
     CDS             14252..16390
                     /locus_tag="ACG81A_000664"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5014057.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(16758..17741)
                     /locus_tag="ACG81A_000665"
     CDS             complement(16758..17741)
                     /locus_tag="ACG81A_000665"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS5014058.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            18118..>18694
                     /locus_tag="ACG81A_000666"
     CDS             18118..>18694
                     /locus_tag="ACG81A_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS5014059.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLA"
BASE COUNT         5908 a         3122 c         3893 g         5771 t
ORIGIN      
        1 aagatttttt ctgacaattt tattatcact gatattaaca cctgtattca aaatcatttc
       61 agtacaaaca ggtatggtcg acaaaccaaa tgaaagaaga ataaataaag ttccaatgcc
      121 ttcagcgggc ggactgccta tctttatttc atttgttata tcgactttat tcttattccg
      181 taaaattatc cctcaatcgt atattgtacc gatcttgatt gcttctttag taatcatagt
      241 cacaggagtg ctcgatgata aatatgagct gacacctaaa caaaaaagta tagggatctt
      301 gattgcttct cttattattt attttgtagc agatatacgt attgattcct tcacgcttcc
      361 ttttattgga tatatccaac taggctggct tagttttccc gtaacgattt tttggatctt
      421 cggtatcacg aatgcggtca atctgattga cggtttggat gggctggcgg cagggatttc
      481 attgatcggt ttaattacga taggaatcat tggatatttt ttcctccatg cttctacagt
      541 gtatattcca gttgttattt tctgtcttgt agcaagtatt atcggttttt ttccttacaa
      601 tttttatccg gcaaagattt atttaggcga tacaggagct ctattcctag ggtttatgat
      661 ggccgtcctt tcactacaag ggctgaagaa cgtgactttt gtttcatcga tttctttgct
      721 gatcgtcatg ggagtgccgg taacagacac gttttttgct atcattagga gaaaagcaaa
      781 ccgtgtttct ttttctacag cagataaaaa acatcttcat caccgactaa tcagtttagg
      841 ctttactcat aaaggtgctg tcctgacgat ttatgcgatg gctttgatgt tttctttcac
      901 agctatggtc atgaattata ctggaagaat cggaacgatc gtattgatta ttgccatgtt
      961 gtttgctgca attttattat ttgaattgat tggtctgatc aatgagaaac atcagtttat
     1021 tttaaaaaca ttgcgatttt tagggaataa agagtaccgt acgcaagtaa aacagaaata
     1081 tttgaagaaa tggaaccgtc atatagatga ataacaaatt aaaccagccg atccttatta
     1141 gtattgtgac ggcaaacagt gataaaattt ttcggacact agataatttt atttcaactg
     1201 taaaagatga aacagcagta aaaatccgaa tttttgacaa caattcactt cccgagtaca
     1261 aaaaaagact aacagagtat aggaaatatc cctacatcga tatctatttt catgatgaaa
     1321 attccggatt tggttatgga cataatcaca atctgatagc tagcgattct cgctatggca
     1381 ttatttgtaa cccagatatt ttagtggatg aagagacagt tacagcattg gtcttgttat
     1441 tgaaagaaca tccggaatgt gcgatgttag cacctaagat tcttaatgaa gatggaacaa
     1501 ctcagcacct catccgtgag cagctggctg tttttgatta cgttttgcgc tttgtgcctt
     1561 tcaaatgggt caaaaaaaag tttgacaaac gactggctgt gtttgaatgc cgaggattgc
     1621 ctactgatgc taatagttat gtacggatga tctcaggtag ttttatggta gctgatattg
     1681 ataaatttaa agagataaag gggtttgatg agcgtttctt catgtacttt gaagacaacg
     1741 acttgtgttt gagttttgaa aaagcaggtt acaagttatt gtacacaccc ttttatgcag
     1801 ttatccattt ttacgggaag ggtgcgcatc gaaatgcaaa actgtttcgc gtgtttttga
     1861 cttctatgat gaaatttttc aataagtggg ggtggcgttt tttctgatga aaccagaaat
     1921 attagtagtc gttgtgcttt atcagcaact attcagtcaa tctccagctt atggaccatt
     1981 aagcaaagca cttagcgaaa agcaaattca gcttattatt tacgataata gtcctcaaag
     2041 gcagcaaaat gagctgtttg aaaaagaaaa tatcgtttat catcatgatc cgcaaaatcc
     2101 tggtttagcg gtggcttata attttgcgtt gtctcaagca gatgaaggaa cacgttgtct
     2161 cgtcactctg gatcaggata cgaggctagt cgacgattat tttgaactat tgcgtaaagt
     2221 aacctttaca gatgaatgtg ttgctgcagt tccgatgatt ttttctggga gccgacaagt
     2281 ttctccccta tattcagatc gttatatcaa tcgtacagct caagcagtag aagttgaagg
     2341 cccaacagct gaacgaatca tggcaatcaa ttcaggtgtt gcctggtctg tcaaatttct
     2401 aaaaaagatt ggcgggttta atcctgcttt ttctttagat tttttggatc attggctgtt
     2461 ttggaaagtg gatcagttgg aaaaaactgt ggagatcctt cctgtacgaa tggaacatga
     2521 tctttcggtt ttagactatg aaaaagtttc aagtaatcga taccgttcta ttttacaagc
     2581 agaaaatcga ttttatcatg aatatgatca aaggcattta tccagtcacc gacgacaatt
     2641 attgttaaga acagctaaac agttcttgac cgtcaaaaac cggcatatct ggcggatgac
     2701 tttgcgctca tatgttaaga attggaaggt gtgaattcgt gcgttcggtt tgtatagcta
     2761 cctttaatgg aggcagatat ttaagagagc agttagatag tattttatct cagctttcag
     2821 cagaagatga agtagtcatt tctgatgatg ggtcagtaga tgatacatgg gatatattaa
     2881 ctgaatacgc agaaaaagat aagcgaatcc agcttttttc ggggcctcgt caaggattga
     2941 tcgctaattt tggttatgcg atccagcaga cgaaaggaga tctaatcttt ttggcagacc
     3001 aagatgatgt ttggttggag acaaaagtga aaaagatcac tgactatttt gagcagaatc
     3061 cggatcagct agtagtagtt tctgatttaa tcattgtgga cagcgagctg caagagatcc
     3121 atgattcgta tttttcgttt cgaaattcca aagaaggatg gcttaataat gtgataagaa
     3181 gtcactatat tggtgctggt atggctttcc ggtcttcttt aaaacccgat attttgccga
     3241 tcccgtcaga agtgccgatg catgatatgt ggattggttt gctgggagga aagaagactg
     3301 ggttcctaag agaaccgtta actctgtacc gaagacatga tttaaacgcg agtgagattg
     3361 aaacacattc taaattacgg caaaaaattt cttggcgtgt tcatctgatt acggcgttga
     3421 tcaaacgaaa atggtttaaa cgttgataaa agatgtttaa atccttaata tttccatgac
     3481 aaatataaca attgatgaga taataacaaa gtgaaatttg aaatcaaaaa ggagagcaga
     3541 tatccatgaa gggaatcatt cttgctgggg gcagcggaac acgtctgtac cctttaacaa
     3601 aggcaacatc gaaacaattg atgccgattt atgacaaacc aatgatttat tatccaatgt
     3661 ctactctgat gttggcggga atcaaagaaa ttctgattat ttcaacgcca caagatacac
     3721 cacgtttcaa ggaattattt ggaaacggaa atgatttagg actgcagatc gaatatgcag
     3781 tacaagaaag tccagatgga ttggcacaag cgtttatcat tggagaagaa tttattggag
     3841 atgatagtgt ctgcttggta ttaggggaca atatttacta tggtggcggt ctgtctaaaa
     3901 tgttgcaacg agcagcttca aaagatacag gcgcgacagt atttggctat catgtcaatg
     3961 atccagaacg ctttggtgta gtagaatttg atgagaatat gcaagctttg tcgattgaag
     4021 aaaagcctgc tcagccaaaa tcaaattacg ctgtaactgg cctttatttt tatgacaatg
     4081 aggtaatttc tatcgctaaa ggtatcaaac cttcagaacg tggagaacta gagattacgg
     4141 atgtcaataa agcatatttg gataaaggaa aactatccgt agaacttatg ggacgcggat
     4201 ttgcttggct tgatacagga acacatgaat cattgcttga agcatctacc tttatcgaaa
     4261 caattgaaaa acgtcagaac ttaaaagtgg cttgtttgga agaaattgct taccgtatgg
     4321 gctacatcga taaggaacaa ttgctggccc ttgcacagcc attgaaaaaa aatcaatacg
     4381 gtcaatattt attgaatttg gccgctgaat aaggagtcct tacagatgaa agtaacagat
     4441 acaaaattaa cagatgtcaa aatcgtagaa atggacgtct ttggcgatca tcgcggtttc
     4501 ttcacagaaa gttattcaaa agcaaaattt gcagaacatg gattgaaata tgactttgtt
     4561 caagacaacc attcactatc agctgaagct ggtgtccttc gggggcttca tttccaaaaa
     4621 ggagaagctg cacagacaaa attgattcgg gtagtcactg gagcagtttt agatgtagta
     4681 gtggatatcc gcaaaggtag cccgacttat ggtcaatggg acggatttat cttatctgaa
     4741 cataatcatc gacagttgct tgtacctaaa ggatatgccc atggattcgt tactttgaca
     4801 ccaaatgtga acttccttta taagtgtgat aattactata atgcagaagc agatgggggc
     4861 atcgcatttg atgatccaga tttagcgatt gattggccaa ttgatccatc aagagcgatt
     4921 atgtcggaaa aagatcaccg tcatccgaca ttaaaagaat ttgaagcaga aaatccattt
     4981 gtttacggag aaatctaaga attaaggaga aattttctta tgaaaaacat cattgttacc
     5041 ggaggtgccg gattcatcgg ctctaatttc gttcattacg tggtaaacaa tcatccagaa
     5101 gtgcatgtca ctgttttgga taaactgact tatgcaggaa acaaagagaa cttggccgga
     5161 cttcctagcg atcgcgtaga attggtcgta ggagatgttg cagatgcaga attagtggac
     5221 cgtctagtaa aagaagcgga cgcagttgtt cattacgcgg cagaatccca taacgataat
     5281 tcattaaaag atcctttccc atttgtacaa acgaatatca ttggtacgta tacactttta
     5341 gaagcttgcc gcaagtatga tgtacgctat catcacgtat ctactgacga ggtttacggc
     5401 gatcttccat tacgtgaaga cctgccagga catggggaag gcgaaggaga aaaattcact
     5461 gcggaaacac catataatcc ttcaagtcca tattcttcta caaaagctgg ttcagatctg
     5521 ttagtaaaag catgggtgcg ttcttttggc ttacgtgcaa ccatttcaaa ctgttcaaac
     5581 aactatggtc cttatcagca tattgaaaaa tttattcctc gccaagtaac gagtatctta
     5641 agcggtattc gtccaaaatt atatggcgaa ggaaagaatg ttcgtgactg gatccatacg
     5701 aatgatcatt cttcagcagt gtggctgatt ttgacgaaag gcagaattgg cgaaacctat
     5761 ctgatcggtg cagacggtga agaagataat aagaccgtga tggaattgat cttagaaatg
     5821 atgggccaac caaaagacgc atacgaccat gtaaacgacc gcgcagggca cgatttgcgt
     5881 tatgcaatcg attcgacaaa attgcgagaa gagctaggtt ggcagcctga gtttacgaac
     5941 ttccgtgatg ggttggctga cacgatcaaa tggtacgaag aacatgaaga ctggtggaaa
     6001 aaagaaaaag aggccgttga agcagcatat gccaaaaatg gacaataata cagaggaaat
     6061 ttcactgtat taatggaaaa acacatgtga ggcaggcgct cgcctgtctc ttttttaaaa
     6121 taaaaatcaa ggagcggata accatatgtt tttattaact ggaggaaatg gacaattagg
     6181 tacagaattg cgccatttat tagatgaaaa aaatctgcca tatgtttcaa cagatgcaaa
     6241 agacttggat atcacagatg ctgagaaaac aatggcttat attacagaat taaaacccga
     6301 tgttattttc cattgcgcag cttacacagc agtagataaa gcagaagaag aagcaaaaga
     6361 attagatgag aaaatcaatg tcgatggcac aagaaatgta gcacttgcag caaaagctgt
     6421 gaatgcacaa ttcgtctata tcagtacaga ctatgttttc gatggaaaaa acaaaaatgt
     6481 agaatacgaa gtagacgacc aaacgaatcc actaaatgaa tatggtcgta cgaaattatt
     6541 aggcgaaaaa gcagtagaag agattttaga agactattat atcatacgaa cttcttgggt
     6601 tttcgggatc tatggacaca atttcgtctt cacgatgcaa cgcctagcgg aaacccgcga
     6661 caaactgact gtagtaaatg accagtttgg gcgaccaact tggacacgta ctttggcaga
     6721 atttatggta tatgtcatcg aagaaaaagc accatttggt atttatcatt tgtcgaacga
     6781 aaatagctgt tcttggtatg agttcgcaaa agaaatattg aaagatacag atgtagaagt
     6841 tgcacctgtt acgtcagaag aatttccgca aaaagcaaca cgtccgcagt attcagtcat
     6901 gagcctgaaa aagacagaag cgttaggttt tgtcatacca acttggcaag aagcacttgc
     6961 gcaaatgctg gaagctgcca aaaaaatgaa taagtaattt cctttttatc aaatagaatt
     7021 tggagcatga tatggaaaaa caaacaagaa gtgcacgacg aaaagaaaag ccaaaagcga
     7081 atcaggcaga ttcgaatttg gtgcttgacc tgtttctaaa actccgctat cttattggcg
     7141 ttttagtcat cgtggtcatc gttactttta atttaaacgg aagttcactt ggtgtttggg
     7201 ataaatatgt gtcgcaaaga gacgatggga aaaaaactga tgtgattttt ggacagaatc
     7261 gggaagtgcg gtcagacgaa tggctggtgc agacaccttt ttacctctca caagctgaag
     7321 aggacttttc gttggtgaac gaggattatt cattaaacgg acaaaacatg atcattgcgt
     7381 ataattctcc tgtaaaggat attacggtta tcggaaaacc attcaactgg ggattctttt
     7441 tcctcggaag agatcgagga ctttcctttt attgggcgat gaaattagtc gtcatggtct
     7501 tgcttggatt cgaagtactg atgatattaa cgaagaggaa aaaagctcta tcgctaatcg
     7561 gtgcatttgc gctgacattt tctccagcag tccagtggtg gttcatgcaa catgtgggcg
     7621 atttgatttt ctttactttg ggactgatga ttgcttttta tcattatttc tacaatcatg
     7681 ataaaaaatg gctacgaacg ttgatgatgc ttttggtggt tattttcggt attgggttta
     7741 ttttagtgat ttacccagcc catcaagtga tgctggcgta cttgctgatt ttttattttg
     7801 ttggcctgct tatttactat agcaagaaag tttcatggga tctgttggat gtagtgttga
     7861 ttggaggggc agttttattt attggcggag tcatggttca tttctggcta acgtcaaaag
     7921 acgcattgtt agcttctttg aataccttat atccaggaaa acgggtaagt actggaggaa
     7981 agtggacgat tggcgaattt ttttctttcc ttacaaattg gaaaattcct tttgaagata
     8041 tcaactattc gaataactct gaagtggccc tgttctatca cttttttcca actgtattcc
     8101 tagcaagtcc atttgtgtta tttggcaaaa aagatagcga acaaaagtta ctgggacgta
     8161 ttttgatgat tttttgtctg ttttctatct tttggattac cgttgggttg ccaaaatggc
     8221 tagcagaaat cactctgctg tcttatgttc ctccattaag agccatcctt acttttagtt
     8281 ttgcggcttg tttattatcg atctggttta tcgcttatat ttggagagaa gatgttattc
     8341 ctatttggta taaattcatt ttgttggcag taaatgcatg gtcttatttc tatgcgatta
     8401 cgcattcgaa aatggataat tatttttcgg attttgaaac catcgcagtt gctgtgttgg
     8461 gaacagtgat tttagctttt gcattatttg gctggagacg cttgttttac ctcgcttttg
     8521 tcgccttagt tatcgtatct ggctttttcg tcaatccagt cgtagaaggt acaggagcga
     8581 tttttgaaaa aacactggca aaagaaatcc aaaaaatcga tcaacaagat cctggtaaag
     8641 tttggttgtc tgaagatgaa ttatataact tcaccccaac attgggagtg aaatccttca
     8701 atacagtacg cttttatcca gatatagctg catggaagaa aatcgatccg aaagaagaat
     8761 acgagaagta ttataatcgg tatgctcata caagagcttt tatcgctaag gaagatacaa
     8821 agttcatttt agatcgtcca gatatgtttt cggtcacgtt gaattttgaa gacgcagaaa
     8881 aattagggat cgactatgtg atcagtaaac gaccattaac tgattacaac caattatatg
     8941 aagcgcagtt cacgcagcta tatggaccgg ataaagacgg ttatatgatt tttaaagtag
     9001 aatatccgga ttatccagat gtaccacaaa cacaaactca ggaaaatcag ctagtcgtac
     9061 agtaataggc tagctgagaa taatcctgaa atattagtag aaaggagagt gaagataatt
     9121 caagcaatcg tacaaaaatt tgtcaacaaa atgagtgtcg gtttcttgct tttgttgctg
     9181 acgctagtct tttttgtttg gacagcaggg gtaggttttc cagcaacttt gtttatatat
     9241 aacaatagtt cattacttat cgcttttagt gtggctattt tgctcatttt aaatgtttat
     9301 aaagcaaaag gcgtcgattt tctttatcta ggtgttgcgc ttgccctttt cattttcttt
     9361 tcttactttc gggaattaag aaatgagtct ttatttggag acttactgtt tcctttggtt
     9421 attttatttt gtttagtaat taaatggatc gagtggacaa aatgggatcg cggtatttat
     9481 ctgattgtct ttacggtctt cctcggaatc acgttatatc gtgtatttac agagatccaa
     9541 gtaccagaag gacaaagcat ctgggctcca ggcaattcgc tttctgatat ttggatcaat
     9601 acgaatacaa tcggaagttc tttgatgact cttgctttat tgatcagtgg atttgccagt
     9661 tcttttgaac gatggtatgt tcgaatcatc ggtgggatag cagtcgttgc agccttttta
     9721 ggtatttggg tctgccaatc tagaggtgca ttagtcgcgt tgattgtttt tgcgatattg
     9781 gatcttttac ctaaatcgtt gatgcgtgtc attcgtgcgc catttattgc ctacacggtt
     9841 acgattcttt tagctttgcc gatttcttat ctggcggctg tctctgaaaa agtcaatttg
     9901 tttaccgatc gagaagatat ttggcataaa ttctatcaga cgctaggaga aaaatctgaa
     9961 caagttttac taggaatgaa aacatttatc ttccagcgag gaaaccaatt tttaggaaac
    10021 cacaatagtt ataattctat tttgaatatc tatggattga taggatttgg gattgctgct
    10081 ttgctgctta tcctttttat tggtcgctta acgttgaaag cagatttatc caacgggcag
    10141 atgactttta tctgggcctt ttttgcggtg atgatgcagt catttatgga ggatacattg
    10201 acttcatacc catgggttcc gattatttat atcctgttgg ctatggccgt tcatcgatat
    10261 gatgaaccag aagttgcgga ggaattctat gttatggaag aggcatatga cgatatgact
    10321 tatgatgata catcttatga ggaacaacca gatagcgctc attcttccag gatccaacgc
    10381 tatcattgat aggcacaaaa tacgagagag gctgtgacaa ccgtcgcagc cttttttggt
    10441 ttattgcaat tgtctagcat cttctgtaaa atagaaacgt tagagaaaat cttgtatcat
    10501 agaaaattaa gaaagaaata agaacgcagc tcggtaccaa aaatatggta aaatggcact
    10561 tgactgtaat tcactgaaaa tcgcttggag gaacacaaat gtttaaagat ttgtatttat
    10621 ttatgaagga catttatcag aacagaaaat tattgctaca gttttcgatc aatgacttca
    10681 aatcaagata tgcaggatcg tttttaggaa tcttatgggc gtttatcaat ccgctattta
    10741 ctgtattgat ttattggctg gtatttggtg ttggactgaa agcacaaatg atcgatgcaa
    10801 aatacccatt tatcgtatat ttagttaccg ggatgattcc ctggttcttc ttctcagatg
    10861 catttgcatc aactacggtt gtttttcggg aatatactta tttagtaaag aaagtcgtat
    10921 tcaatattcg tatcttgccc acgacgaaaa tcctatccaa tctgtacacc catttattct
    10981 tcattctgat tggcatagct gtcgcgctta taaatggtat atatccttca gcaatgtctt
    11041 tacagctgat ttattattta ttttgcatgt gtgctttctt gacaggattg acttggctga
    11101 cggcatcgat ccaaccattt ttacctgata tcatgcaatt aattacgatc gtgctgcagt
    11161 tgatcatgtg gacattaccg atattatgga gccctaatca gtttgattca ggtcttgtaa
    11221 gcatattgaa attaaatccg ctgtactatg ttgttcaagg ttatcgtgag tcatttttaa
    11281 gtgaagcttg gttctgggag catggaacat atactttgta tttctgggtt ttcacgttag
    11341 taatgttcgt aatcggctct gcagtgttcc gtagattgaa accacatttt tcagatgtgc
    11401 tgtaaggaga atagctatga cagaatatgc aattagacta aaagatatca caaaatctta
    11461 taatatgtac gcaaaacctt cggatcgatt tcgtgaagcc ctcaatcctt ttaagaaatc
    11521 ctatcatgat gtattttatg cgttgaaaaa cgtgaatgta gaaataaaaa aaggtgagat
    11581 gatcggtttc gtgggagaaa acggatcggg aaaatctaca atgttgaaaa tcattacggg
    11641 agtactgaca ccaacatccg gcacgatgga aatcgaagga aaaatctcag ctttgctaga
    11701 actaggatca ggattcaatc ctgagtattc tggctatgaa aatatctatt taaatggaat
    11761 ggttctagga ttttcaagag aagaagtcga tgaaatggtc gatgatatca ttgaatttgc
    11821 tgacatcgga gaccatattt atcaacctgt aaaaacttat tcaagcggga tgttcgttcg
    11881 tctagctttt gctgtagcaa tcaatgtgaa tcccgatatt ttgattgtgg atgaagcatt
    11941 ggcagtaggc gatcttgaat ttcaactaaa atgtatggaa aaatttacag aaatcaagaa
    12001 ctcgggaaag acgattttat tcgtttctca tgatatcagt tcgattcgtc gtttttgtga
    12061 tcgttcctat tggttgaaaa acggagaagt cgtcgaatat ggcgatacga tggaagtaac
    12121 gacgaattat gaaaactttt taaagaaaaa atcagttaaa acagtggatc ggaataaaaa
    12181 ccaagtagaa cggttcgccg ctccagatat cgtcgacgtg acgaaagcgg aattgttgga
    12241 taaagacaaa aatccggtgg atatgctgga acaaggagaa aaactatacg ttaaagtaac
    12301 ttatgaagta aaagatgata caatcaaaaa tccggtttta ggaattgcgc tacgaacagt
    12361 cgacaatttt tatgtatgcg gacttaatac tctactagat ggtatcaaga ttccttggaa
    12421 aaaaggagag aatatctttt atctggagta tgaaaagtta ggtttgttag ctggtgaata
    12481 ttattttgac gttgcggtct ttgaagaaaa tgcaacggtt cctttggtat ataagacaaa
    12541 gtacatgaac ttgtttgtca gcggttctta tatcggagaa ggcatcgtgg tcttagacca
    12601 taaatgggaa gaagggactc atagcaatga aatatgattt tgaaatggac ctagatgaac
    12661 aaagcagtgt gggaaaaatt gccgcacaga tcaaaccagg aagtaaagtt ttagagtttg
    12721 gtcccggaaa cggccgatta acgaaacatt taatcggagc aaaacaatgt gaagtgagca
    12781 ttgttgagtt agataaggaa ttgttcgatt ttgtcagcga atttgctcaa gatggatttt
    12841 atggtgatat cgaaagtttt gagtgggcaa actattatgc tggacagaca tttgattacg
    12901 tcttgtttgc ggatgtttta gaacatttgg tagatcctgg aaaaacattg aagaaagtca
    12961 gagaattttt gaatgaagaa ggagagatcc tgattacatt ccctaacttg gcgcataatt
    13021 ctgtcatgat cgatttgttc aataaccaat tgccgtgggc ttcttatggt ctcctagatg
    13081 aaacccataa ttctttttat actcatgatg gcttccaaaa agtttttgaa aaagcgggat
    13141 tattcattaa tattgaagat tatttgtact tagctgtagg tgatacagaa ttaaaatcaa
    13201 cttatgaaga attgccggaa gctgtacgct atgatttcaa aatgcgacca tttggtgaag
    13261 tctaccaata ctttttctca ttgatgaaac atccagtagc acaaagttcc attgcagagc
    13321 cacaaaattc taattatgta aaagtgttag aagtaaccca gcaaacgaaa caagatgaaa
    13381 caatccaaca aataccattc aataatttca caggggaaaa tgagacacta agtttccccg
    13441 tatcagaaac aacagaaaga atggtgtttc gcttcgcgca acaacctagc tttatcgaat
    13501 tcagcgcaga agctgccgga gaaaaactaa catttatcga ttcgaatgct gtagtcaaaa
    13561 cagtaaatga ttgttatttg tttgatggga aagaattacc agagttcgtt ctaacggata
    13621 tttctgggaa agaagtgact atccattgtc actatcgctt tatcggggaa ttgacaccaa
    13681 caatgaaaga attgttggaa acgattcgtc ccatggctga agtcacaaag cagctttctg
    13741 agaaaaatga cgaattagaa agagaaaatc atcatcttct tgaggaaaat acaagactag
    13801 accatacgtt aaaaacaacc acaaatcgct attgcacatt gttggaaagt gatgaatgga
    13861 caataaaaca tagactagga cgacgtaaga aagaaacttc gaaaaaaatc caagaaaaag
    13921 aactttctat atgtattgac gagaaaatct gggatgcaga aacaaaaata ttaaaaatca
    13981 tcggatgggg aatcgctaat tcggatcgcc agcccttgtc ctataagtta tcagctgacc
    14041 aatcgccatt tttcgaagca attcctgttt cacgagaaga agtcaatcaa gcagaacagc
    14101 tcgcaaaagg aacagaagcg ggatttgagt tacgaatcct ttgcgagcaa gaacgttcat
    14161 tcttagtgga agcagtagct caaaatggcc aatcatggat cattgaaatg taaataatga
    14221 ttaacttata aagataagga gtaaacgaat cgtgcaaaaa aaagaaattg ccgtatttat
    14281 cgatcaaatc actcgtgaac gggccactgg cgatttgctt attgtgggct gggcaattga
    14341 tgaagtgacc aaagaaatcc ctacgataaa ggtagaaaaa gaaaatgtga ttgctgaagc
    14401 aacacatgtc gtaagattgg atatcaatca tttatataat cttgatgtta agacccaaag
    14461 cggtttcaag gtacgtttat cagggaaaat gagaggaaaa gccatcttag acttccaaac
    14521 agcgagacat caaaatggca tagctgtgaa attaaacggt aaataccctt atgatgacgg
    14581 cattgaatct tcatgggaaa gaaaaaaaag attactgaaa aaagggataa attatgcaag
    14641 gactcatggc gtaaaaaaag cgatccgtcg ggtcaaattg gaattgaagc caggatcgat
    14701 cgactatgca gagtggatca gtcgtcatga gaaaccagat ttgaaagagc aaaggaaaga
    14761 agcacaagga tttgcgtacc gtccattgat ttccatactt atgcctgtct ataacgttga
    14821 aatcaaatgg ctcgaaaaat gcattgattc ggtactggat caaacgtatg atcattggga
    14881 actatgtatc agtgatgatg cttctactga tccagctatc cgaaagtgtc tggaatccta
    14941 tcaagcaaaa gatgatagaa tcaaagtcgt tttccgaaaa gaaaatggac acatcagtct
    15001 ggcaactaat tctgcattgg aaatagcaga aggagaattt atagcactct tagataatga
    15061 tgatgaatta ccgccatttg cattatatga agtggcaaaa gtgctaaacg tacatccaga
    15121 actggatgtg atctacagtg acgaagataa aatcgatgca gatggcaatc gctttgatcc
    15181 gcatttcaaa gcagactggt cgcctgatac attgatggga aataattata tctctcactt
    15241 aggcgtatat cgaacaagca ttgttaaaga acttggcggt ttccgtaaag gctatgaagg
    15301 ttctcaagat tatgatttgg tccttcgtgt gacagaacaa atccctgcag atcatattta
    15361 ccacattgac cgagtcttgt atcattggcg gacgatccca ggatcaactg caagtaatgg
    15421 cgaagccaaa agttatatct atgattccgg agtgaaagca ttgacagatg ccctgtctag
    15481 aagaaacatc aaaggaagcg tacgtcctgg cagaatttct ggattttatg agattgctta
    15541 tgacgtcttg caagaggatt tggtcagtgt gatcattcca acgaagaatg gctatgaaga
    15601 tctaaaaact tgtgtggatt cgatcatcga aaaaacgtct tattcaaatt atgaaatcat
    15661 tattgcggat aacggaagta cagatccaaa aatgcaagaa ttatttgcag aatacaagca
    15721 tcagctgaaa gaccgcttta tcgtagaatt gattgacatt ccattcaatt attctcgaat
    15781 taacaattta gcagcagaaa aagcaaacgg caaatacttt ttattcttga ataacgatac
    15841 agaagtgatt gagccagatt ggatgacggt gatggtttct tatgcacagt ttgatcgaat
    15901 cggctgtgta ggagcaaaac tgttttatcc agatgataca acgcagcatg caggagtatt
    15961 attaggaatc ggtggtgttg cagggcatgc attgaataac tatgatcgaa cacattgtgg
    16021 ttatttcggc cgattggtca ttgacgtcaa ttacttagca gtcactgcag cttgtatgat
    16081 ggtcaaggca gctgacttca atgctgttaa cgggtttgat gaaacattag aagtagcatt
    16141 caacgatgtt gacttgtgct tgaaagttta tgaactgggt cgttacaatg tctatgcaca
    16201 tcaagcagaa ttgtaccatt tcgaatctaa atcaagaggt tatgaagata ctccagaaaa
    16261 acaaagacgt tttgctggtg aaatcaaaaa aatgcaagat aaatggccag catatattgc
    16321 gcatgatcca ttttacaatg ataatttaac aaaagacgga atcggcgact tttcgttgag
    16381 accagattaa actataataa aaagctgtta tgttgtgatt cgctcgcaca taacggcttt
    16441 ttatagtttt ttgtcaaaaa aaggcagaag gtgccgactc aaaaaaataa gtcgaggttg
    16501 ggacagaagt gtttaactcc gagaaataag aagaaattca cgaaaattgc ttttcaaatt
    16561 tttgtgaatt tcggcttatt taagaagaag ttgcttctgc tcccaccgtt tattcgtttt
    16621 taaagaatga gacaaaagtg tttcttactt ttgtcccacg ctcttattca tgctcagagc
    16681 gccaatagat acaaaatcat agccaaagtt tcacgaaccc tagctgtcaa agaatcgata
    16741 ttttatttta atataggcta ttttaaaata aatggtttaa ggtattcctc tacaaaagcc
    16801 actctatcaa ttatcatggg tttaaaataa gaagtgatgg agataaccag attgttctta
    16861 gcgtcaatat aaagaacatt tccactatta ccaattgctg aatatatttc gttctcagaa
    16921 tctaataccc accataaata gccatatctc atattttgta agtcttctaa agcactggtt
    16981 ctaggtttga acatctcgtt tatccacatg ggtggtacga tttgttttcc tttatacatc
    17041 cctttattta ggcataatac accaattttt gccagatcac ttgccgataa acacaaacca
    17101 tatcctgccg tcccgatatt tagtggatca caaaaccaga cattggtttt aggagactta
    17161 tccattgtaa actgcttatg ttcttcagct gtttcagcat aataattgac atggtttggt
    17221 atccctatag gatcaaatag atacttattg gcaaaatcaa cagttgttag cttacttgtc
    17281 tgatacaaca agccagttaa tatgtgtaaa cagacagttt gatagttgaa ttcgccggtt
    17341 ataccttttc ttcctcccaa taaatctagt gatgtttttg tccaatcttc acttgaacag
    17401 acttttgtcc atggatcgcc tttacatttg tagggtgctt tcattgttag caagtgcttg
    17461 attgtaatag cgtaaatagt tttctccccg cgtttcactt tatactctgg aaaataatct
    17521 aatactttat catcgatact tttgattaat ccttgctcta aagcaatgcc aatcagcaaa
    17581 gatacaatac ttttcgttgc tgacatgata tgacaagtat cgtctttttt ataaccattc
    17641 cattcatctg tataaacttc taccccatcc ttatatgcga caatttgaca tatatttggt
    17701 tgtcgaaaac caataaaatc atgcaactct tgtttattca taagatatta cctcactttc
    17761 ttctataagc ataatgtcat taaaaatcga tttcaatttt ttcgattctt cacgtaatac
    17821 aggtattctt atggactaga cagcgctttg ggaagatgga ttctctgaaa aatataaagg
    17881 ttcactttat gagtggataa aaaaagcagc aaatacttcg acttctttaa tttaccaaca
    17941 ttagtgaaaa tcgattcatc tggagatttg taatttagag tccttgtctt gattagaggt
    18001 gtataaagct ttttattaga catttttaaa ttccattaca atttgcttat aaactaggtt
    18061 tttaaaggtg actatggtaa gataatagag atatattttg tgattaggag tgtatatatg
    18121 aataaaaatg gagaatggag tgctgcacga cggatattca ttatattaat ggatgttatt
    18181 gtatacaatg cagcaatata tagtagtttc ttactgaagt ttcgtggaga gatcccatta
    18241 cgaaatttag aaactttcca acactcagca attattattt cagtgatttt tattgcgttg
    18301 aacattttgt tgggtgcata tgttttttat aatcgaatga tcagtgacat cgtgtttgtc
    18361 acagtcatcg gtcaggtatt gatgacacta gggatcatgg tagtgacttt tgcgggccgc
    18421 tggtttgctt ttccacgtct tgttatctta ctttcatttg ttctcggtac gcttttctta
    18481 agtatctatc gtattctcgt atataaactt tatttacgcg taagtcgaga taaaaaggtc
    18541 gtcattattg ggttagaaaa ggatgtcgca ccagcaatcc aaaattttgg ttcaaagaaa
    18601 aacaacaaac ataaagtcga agcagtcatt cttgataact attatgcaaa catcaaaaat
    18661 atgattaaag acatcgatat tgtctatctt gctt
//
LOCUS       DAZULY010000028        18549 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000028 DAZULY010000000
VERSION     DAZULY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.028"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..487)
                     /gene="yajC"
                     /locus_tag="ACG81A_000667"
     CDS             complement(107..487)
                     /gene="yajC"
                     /locus_tag="ACG81A_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFS5014060.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(584..1729)
                     /gene="tgt"
                     /locus_tag="ACG81A_000668"
     CDS             complement(584..1729)
                     /gene="tgt"
                     /locus_tag="ACG81A_000668"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFS5014061.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1947..2645
                     /locus_tag="ACG81A_000669"
     CDS             1947..2645
                     /locus_tag="ACG81A_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS5014062.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2813..5452)
                     /locus_tag="ACG81A_000670"
     CDS             complement(2813..5452)
                     /locus_tag="ACG81A_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5014063.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6071..7102)
                     /gene="queA"
                     /locus_tag="ACG81A_000671"
     CDS             complement(6071..7102)
                     /gene="queA"
                     /locus_tag="ACG81A_000671"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFS5014064.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7295..8470
                     /locus_tag="ACG81A_000672"
     CDS             7295..8470
                     /locus_tag="ACG81A_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5014065.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8474..9115
                     /locus_tag="ACG81A_000673"
     CDS             8474..9115
                     /locus_tag="ACG81A_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5014066.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9112..10029
                     /locus_tag="ACG81A_000674"
     CDS             9112..10029
                     /locus_tag="ACG81A_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5014067.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10026..10697
                     /locus_tag="ACG81A_000675"
     CDS             10026..10697
                     /locus_tag="ACG81A_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5014068.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11238..11726)
                     /locus_tag="ACG81A_000676"
     CDS             complement(11238..11726)
                     /locus_tag="ACG81A_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFS5014069.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11927..12193)
                     /locus_tag="ACG81A_000677"
     CDS             complement(11927..12193)
                     /locus_tag="ACG81A_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS5014070.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12193..12456)
                     /locus_tag="ACG81A_000678"
     CDS             complement(12193..12456)
                     /locus_tag="ACG81A_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS5014071.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12977..13117
                     /locus_tag="ACG81A_000679"
                     /pseudo
     CDS             12977..13117
                     /locus_tag="ACG81A_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(13259..15082)
                     /locus_tag="ACG81A_000680"
     CDS             complement(13259..15082)
                     /locus_tag="ACG81A_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5014072.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15680..18385)
                     /locus_tag="ACG81A_000681"
     CDS             complement(15680..18385)
                     /locus_tag="ACG81A_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFS5014073.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT         5289 a         3930 c         3475 g         5855 t
ORIGIN      
        1 tcaatattga gcaatatatc ccatgctctt atctctttct tttttgaata aaaaatgagg
       61 taggacatct ttctgcccta cctcgtcttt aaataagctt tgatacttat ttttcttcct
      121 tctcttctgt ctcttccagc gtttctgttg tttcagtttt tacttcaact gtctcagctg
      181 gttcttctgt tgtttcaaca gtagtcgttg catcatttgt tacgacagtc cctggtttga
      241 ccgttttgat tgctgcacga tcgtactcaa ggaagatccc ttcgcagtct aaaacaactg
      301 tccgtttgtc attgtcgatt tctgaaacaa ctccatgtaa tccgccgatc gttacgactg
      361 aatcacctgg tttcatgcta tctaacaagt tttgacgttc ttgctgttgt ttcttttgcg
      421 aacgtgacat aaaaaaccac attgctaata gcgcgataaa catcacaagc attggtaatg
      481 ttcccatttt tttcacctca ctaattctgt atctacaaaa aacactttag cagaaaaatg
      541 ctgataacac tagtttttta cttaattttt aaaagatttt cctttagaag ctttttgcgt
      601 tttctttatt gaacccgtat tcttcaaaaa aggcttctct aaattcaagc aaattatcat
      661 ccatgattgc ttgacgtact tgtttcatta gattcaacaa gaagtataaa ttgtggtaag
      721 aagtcaaacg aatcccaaat gtttcatcac atttgatcaa atggcggata taggcacgtg
      781 tatagttttt acatgtataa caatcacatt tctcatcaat tggacggaaa tcatgggcat
      841 atttcgcatt tttcactact agacggcctt gagaagtcat acatgtgcca tttcgagcga
      901 ttcgagtagg caagacacag tcaaacatgt ctactccacg gataacacca tcgatcaatg
      961 aatcagcagc accgacaccc atcaagtaac gtggtttgtt ttcggggatc aacggtgtag
     1021 tgaattctaa tacacgattc atttctgatt taggttcccc gacagataac ccgccgattg
     1081 agtaaccagg gaaatccatc ccgactagat ctttcgcgct ttgacgacga agatcttcaa
     1141 accctgctcc ttggatgatt ccaaataatc cttgacggtc tggattagca tgagctttca
     1201 atcctcgttc tgcccatcga gacgtacgtt ctactgattt tttcacgtag tcgtaacttt
     1261 catcgaatgg tggacattcg tcaaaactca tcatgatatc agagcccaat ttattttgga
     1321 tgttgattgc tttttctggt gataaaaaca tacgcgaacc gttcaagtga tttttgaaat
     1381 gcacgccttc ttcttcgata ttccgcatat cacttaatga aaaaacttga aagccgcctg
     1441 aatctgttaa aatcggttga tcccagttca taaatttatg cagtccgcca gcttcttcta
     1501 ctaagtcttc accagggcgt agccataaat gatatgtatt acttaaaata acgccagcac
     1561 ccatttcttt caactcttct ggggacattg tttttactgt agctaatgtt cctactggca
     1621 tgaacattgg tgtggggaaa gttccgtgtg gcgtgatcaa ttcccctaaa cgcgcgccag
     1681 tatgtttttc tttcttgatt aaacgatagc gaatggcagg ttgcgtcatt catttcccta
     1741 ctttctatat aaaatatctt gacaactccc accatcataa tcgtttcaag tgctttttgc
     1801 aagttccctc ggcttccttc tactttattt aatattcttt tttttatcct ttttttatga
     1861 ataacaaaaa aaagaaaaac attatgacag aaatattttt tgatacatgg tatattggtg
     1921 ttattaaaga aaaggaggga aaaatcatgc aatcttctgc agaattaaaa cgccaagcaa
     1981 aagagagtct aaaaggacgt tggggacaag ctgttttact aaatctaatc cctacattga
     2041 ttggtattgc tactattctt cttattatga ttcctattat cttgtttgcc accaccatat
     2101 atgatggatc agcgagcgtc tcagaagcga caacttcagg aggcggtagt tcaggaggcg
     2161 gtggcggaat tatttccagc atcattagcg ctttgttcat gagcggtatt tcttggactt
     2221 atcttgatat tttacgtgga caaaaagatc agatcgaacc attcaaagat gcatttcgtg
     2281 gattcaaagg tttgtttatc ggcggagtga tcagcttagc tgtgctgatc tcgatcttta
     2341 caacattatg gggtttgcta ttagttattc ctggaatcat taaaagctat tcttattctc
     2401 aaagctattt tatttactac gatatcgtcc aagaaacagg acaaacacct aaagtactgg
     2461 atacgatcac tgcaagtcga aaattgatgg atggttataa aggacaactt ttctggttag
     2521 atctttcatt tatcggctgg catatcttag cattagcaac tgctggtatt ggttatctat
     2581 ggctcagccc atacatttct gcaacaaaag cagcatttta tgaacaattg ccaaaagata
     2641 tttaaaagag agtgtgactt gtgaccgagg gggtggcagg agaacaccgt ttatcgagag
     2701 gcagtaaaag aaacattcag cccattagca aaaaggaggt gtgacaacat tcatgtcaca
     2761 cctccttttt gtatagttcc agtaaaaaga aaatcaactt agaaacagtt aatcattcct
     2821 cttcttctgc ttccggtaat cgatggaact tgatcagttc attcaccgcg attggtacga
     2881 tagataaaat ccctaccaag aaccagtgat tcaaactgat gtgtactagg ccgaatagtt
     2941 cttgcgtaaa tggcagcaaa gcaacagccg ttgtaatagc taatgccagt aacgccattt
     3001 ctaccaaagc tttattggtc gctaaattga ctttgaagat cgattggctg ctgcgtacat
     3061 tgaacacatg cagaatcgat gaataagcta agatcaagaa tgcaacagtt tgaccgacat
     3121 gctgacttgc gtctacccat gggctgatag tatcgacata actacctaaa taataaccaa
     3181 acaaggttac gatggtgaag acccctgcat ttatggcaat tttttgcaat aaacctcgag
     3241 caaagatccc ttcatttttc ggaatgggtg gctgatccat gatgccgtgt tccgctggct
     3301 ctcttcctaa agcaaatcct ggaagtccat cagcaactac attgacaaac agtagttgca
     3361 cagctgtaaa tggtgctccc catcctaaca gcatcgcaat caaaacaata aagatctccg
     3421 aaatattaca gcttagcaaa aagttgatag ctttacgaat attctgataa accgaacgtc
     3481 cttgactgac agcgtctacg atcgtcgcaa agttgtcatc cgtcaaaatc atatctgcgg
     3541 cactttgcgc cacatctgtt cctgtgatcc ccatggcaca cccaacgtta cttgctttca
     3601 acgctggtgc atcattcaca ccatccccag tcatcgctac gactgcaccc gtacgttgcc
     3661 aagatttgac gatccggatc ttgtcttctg gcgttacccg agcatagaca gagagagctt
     3721 tgacacgcgc atctaattct tcgtctgata attgatgcag ttctgatcct gaaagtgctt
     3781 ctgacggatc attcaagata cctaattctt tcgctatggc actggcagtc accacatgat
     3841 ctccagtgat catgactgtt ttgatgccag cctttttcgc tcgcttgatt gctcctttac
     3901 tttctggacg tggtggatcg atcattccga tcagaccaat caaacgtaag tctttttcca
     3961 gtgcttcaga agaaatatct tgcggttctt cgtcataagt agcataacca acagcaatca
     4021 ctctcagtgc ccgtttacca aagcgatcat taacgatcgc cgcttgttct acatcgcctg
     4081 aactaaagcg cggagcaaga acatcgaatg ctcctttggt gattgagatg tatttttcgc
     4141 ccatttggtg aactgttgtc atcatttttc gttcagaatc aaaaggaagc tcgtttactc
     4201 gtggatattt ttcttctaat tctgcctttg tatgataatt ctcttcaaca gcccttacga
     4261 tggctacttc tgttggatta cctttgataa caaggtcgtc atcttcttgc tcgaccgtca
     4321 catctgtaca tagtgcagcc attttcaata tttccatcgc gctgttcgtc attgaatctt
     4381 cgatattcgt tacttcatct tctttgctcc atacccggcg gacgcgcatt tcattttgtg
     4441 tcagcgttcc tgttttgtct gagcagataa cattagcggt tcctagcgtt tcaactgctg
     4501 gcaactgtcg gatgatcgca tgtttcctcg ccatcttctg cacaccgtat gcaagtgtca
     4561 acgtaacaat aacagtcaag gtttccggta cagctgcaac agccaaggaa atcgaagtca
     4621 tgaacatctc aaggagtggt tctccttgca gttcaccaat cacaaatact aaagccgcag
     4681 ccgctaaagc gatcatactg attcgtttgc ctagctgatt caatcggacc tgtaaaggtg
     4741 ttttttgcat agtattttcg ttcaaaagat ccgcgatctt acccatttct gtagccatgc
     4801 caatggcagt gacgatcgct ttcccacgac ctgcagcaac cgtacatcct ttaaatacca
     4861 tattaagttg gtcacctaat gactcatctt cttcagcaat aaatgctgca tctttttcaa
     4921 ctgcttcact ttctccagtc aacgccgatt cttctacacg taattgtgat gcttcgatca
     4981 aacgggcatc ggcagaaata gcatcaccgg actctaaaac aagaatatca cccactacca
     5041 gttgctctga ctcaacagtg gtcactttgc cgtctcgtat gacagttgcc gtttgtttat
     5101 tcatatcttg caaggattcc agtgcttttt ctgcgtttcc ttcttggaca atcgccaata
     5161 ccgagttgat cacaacgata gcgatgatca gcagtccttc aaataaatcc ccatgttctg
     5221 tagcaaatgc ggtataaaag gaaatcaccg ctgctacaag aaggatgatc gttgtaaaat
     5281 cggacaagct tcgaaagaat tttttgatca tcgattcttt cggcgcctca tcgaatttat
     5341 tcaatccctg cttttttcgc cgttcatcca cttcttgtga agacaaccca gtatctggat
     5401 ctgcttctgt taccttcttt acttcttcta ttgtctgctt gtattcttcc atcctattcc
     5461 tcctttaaaa tcttcgtaca aaaaaaggta cttattttac tataacatcc tttttttatt
     5521 tacttcaaat aaagggctta tcgattattt gttaagaaag cgtaaaattt aaagtgctta
     5581 tatttgcaaa cgctaccaaa ggatgctatt atatagatgt aaggaaggag gataccatca
     5641 aatagacaga agaatttctc gatagaacag tgtaagcaaa caaagttgtt agaagtacag
     5701 gtttcaccca gacatgcgtg tagagtacaa acctataaat agtgaatgcg tatggatgtt
     5761 tggatcacga agtatccgaa acaagaagta atgcgatacg taacaattga ataacgtaaa
     5821 cgtctgaaaa ttgaatagga acaagagatg tttcaaatgt tgaagatgta acgaaaacaa
     5881 aatttttgtt taacatgaag aaattcaaca ccgtgctttg catgtatgat ggaaagttac
     5941 aatcatttct gtttatttga atccttccga ggtacaagaa agccgagctt atcgagagct
     6001 cggctttttc tttgtcataa aatctttgta aagctttttt gttattcaaa actagcggct
     6061 tttcataacc ttatttcacg aacatcgcat ctccaaaact aaagaaacga tagcgctctt
     6121 caatcgcatg ctggtaagca gataaagtca attctcttcc tgcaaaggca ctaactaaca
     6181 tgactaaagt cgattttggc aaatggaaat tagttgagaa agcttctacc actttgaatt
     6241 catagccagg cgtaatgaaa atatcagtcc atccgctgtc tgctttgatt tctccattaa
     6301 atttcgttcc aatcgtttct aatgttcgaa ttgaagttgt tccgacagcc acgattttcc
     6361 cgccattttt acgtacttgg ttcaatcgct cagccgcttc ttccgtcaaa cggtagaatt
     6421 cactatgcat atggtgttct tcaattttat ctacgctgac aggtcggaat gtacctaacc
     6481 ctacatgtag tgtcagatag actaattcaa caccctttgc ttcgatctca tcaagtaatt
     6541 ctttcgtaaa atgcaatcca gctgttggcg ctgcagcaga accattttct ttcgcataga
     6601 cagtttgata gcgatctggg tcttccagac gttctttgat atatggaggc aatggcattt
     6661 ctcccaatga ttcaagaatc tctagaaaaa ttccttcata ctcaaagtca atgatccgtc
     6721 cgccgtgttc caattcttct ttcactactg cttgcaatct gccatcacca aaagagattc
     6781 ttgtcccaac tttggcacgt ttcgcaggtt tgatcaatgt ctcccacgta tctccttgtg
     6841 tattcgttag aagaaggact tctaaatgcg caccagtatc tggtttttct ccatataaac
     6901 gagcaggcag aactcgtgta ttattcataa ctagcgcatc accaggctgt aactcttcaa
     6961 tgatttcatg gaaatgttta tcttccatct ctccagtttt ccgatccaaa acaagtaaac
     7021 gagaatgatc gcggtctttt aatggtgttt gtgcgattaa ttcttccggc aagtcaaaat
     7081 caaaatcttc tgttgttaac atatcgtcac tcactttcta tcatgtgctt catcattcta
     7141 tcatgttttt cgttctgttg gcaaaatcag cactttttca cttctatttt ttgttttcct
     7201 aaataaaaaa actaccctat ttgtattttt tcattgtttt aggcatacta gtaatagata
     7261 gttcttcatt gaaaatagaa aaagaggtgg caccatgatt gaatttcaaa atgtgtcaaa
     7321 aatttataaa ggtggaaaag tcgctgtaga ggacgtcaat ctttcatttg aaaagggtga
     7381 gttcatttgt tttatcggta cgagcggaag cggaaaaacg acgacgatgc ggatgatcaa
     7441 ccgaatgact gatccaacac aagggaaaat tttgattgat ggaaaatcca tccaagaaat
     7501 tgatccagtg gaattgcgac gtcagatcgg ctatgttatc caaagtattg gattgatgcc
     7561 tcacatgacg atccgtgaaa atatcactct tgtccaaaaa ttgttgaaag tcagtcaaga
     7621 agaacggaat aaaacagctg aaaaaatgat tgatcttgtg gaattgcccc gtgagatgtt
     7681 agatcgctac ccacatgaat tatctggtgg tcagcagcag cgtattggag tcgttcgtgc
     7741 ccttgcagcg aatcaagata tcatcttgat ggacgagcca ttcggtgcgc ttgatccaat
     7801 cactcgtgat tccttgcaag accttgtcaa agatttacaa gagcgtttgg gaaaaacgat
     7861 cgtatttgta acccatgaca tggatgaagc gattaaacta gcaagccgta tcgccatcat
     7921 gagtgaaggc cgtgtgatcc aatttgatac accagaaaat attttacgtc atcctgcaaa
     7981 cgatttcgta gaagagttga ttggagaaga tcgcctacta caagccaagc caaatgcgac
     8041 aagagtcggc gaagttatgc taaatacagc aatcacgatt acaccggaaa aatcattaca
     8101 agaagcaatc aaactgatga gggaaaaacg tgtagatacg ttgttagtta ccgataattc
     8161 gaatgtatta aaaggattta tcgatgtaga aacgttgaat aaaaaacgag gaaaagtatc
     8221 cagtgtcggc gacattttga ataaacatgt tttttacgta aaagaatccg cctatcttcg
     8281 tgataccttg caacgcattt taaaacgcgg gctgaaatac gtgccggtcg ttgatgaaca
     8341 gaacaaagtc gtaggtatcc tgactcgtgc atcattagtg gatatcgtct acgatgtgat
     8401 ttggggagaa gatcccgcaa ctactgatgt tgcagagtca gctactcctg aagaatctaa
     8461 ggaggtctga cctatgcagc agttttttca tcagcacggt tcagaaattc tctcaaaagg
     8521 ttttgaacat ctttatattt caggacttgc tttgatcatt ggtatcatca ttgctgtacc
     8581 gcttggtgtc ttactgacac gttttccaaa agctgccacg attgtgattg gcttaaccag
     8641 tgccttgcag acaatccctt ctcttgcttt attggcattg atgatcccgc tatttggcgt
     8701 aggtaaatta cctgcgatta tcgcactctt tatttattca ttattgccga ttttacgtaa
     8761 tacgtacatt gggatgaaaa atgtcgattg gaattatcgg gatgtggcaa aaggcatggg
     8821 gatgactgag ttccaatcca ttttttccgt tgaattgccc attgctatgc caacgatcat
     8881 ggctggtatt cgtttagctg ccgtttacgt gattgcatgg gctacacttg cctcttatat
     8941 cggcgctggt ggtttaggcg atttgatttt tagtggactg aataattacc aaccagatct
     9001 cattttggca ggtacgatcc ctgtaacaat tttagcacta ttggctgatt ggcttctagg
     9061 tctcttggaa caccggctga caccgatcgc acttagagag gagaatgaag aatgaaacgt
     9121 atactaaaaa tgaccatggt tcttttctct cttcttatac ttgcgagttg ttccttccct
     9181 ggtcttgcca gcaatacaga tgaagacacc atttcgatca caggtggaat cacttcagaa
     9241 gcacagatct tagcaagtct agttgccgga atggttgaac attatacaga taaaaatacc
     9301 gcaatcatca acaatctggc tactacaact atcaaccatc aagcgatgat gaacggagat
     9361 gcttcaattt cagcggctcg ctacacagga acagatttaa cgactacatt gaaccttcct
     9421 cctgaaaaag atccgaaaaa agcgtttgct accgtgaaag atgaatttga gaaaagatac
     9481 ggtcaaacgt ggttcccgtc ctatagtttt gaaaatacgt atgtctttct tgttcgaaaa
     9541 gacaccgcac aaaaatacca tctatctaaa gtcagcgatc tgaaaaatgt agcggatgaa
     9601 ttagtagctg gtgtagatac ttcttggatc aatcgtaaag gagacggtta tgacggcttc
     9661 caagaaactt atggcttctc cttcaattcg atattaccta tgcagattgg acttgtttat
     9721 gacgcagtcg aagcaggaaa aatggatatt gttcttggct actctacgga tggacggatc
     9781 gccagttatg atctagtcat gttggaagat gatcgtcaat tcttcccacc atatgatgct
     9841 gctccagttg tagacaacaa gatattagct gatacgcctc atttaaaaga agccttaaat
     9901 aagttaggtc ataccatcag tacggaaaaa atgcaggaat tgaactacga agcagataat
     9961 aatctagtgg aaccatcagt agtagcacaa cgtttcttac aagaaaacca ctattttgaa
    10021 gggaagtgaa gcaattggat aaaatgaatg tctttcaaca atttttctat tatttccagg
    10081 aaaatggctc ctatatcttt gcacaattta tccgtcactt cctgatttct atctatggtg
    10141 tcctctttgc agcagttgtc gggattccgg ttgggatcat gatttctagg agacgtaaat
    10201 tagcaaactg ggtgattcgt ttggcgaata tcatccagac gatcccttct ctagccatga
    10261 tctccatttt aatcattgga ttaggcctgg gagtcaatgt cgtgattgtt actgtatttt
    10321 tgtattccct cttaccgatc atcaagaata cgtataccgg gatgatccaa gtagacaaaa
    10381 acatccttga tgtcggcaaa ggcatgggga tgactgctag acaacgacta ttcatggtag
    10441 agttaccact ctctgtttct gttatcatgg caggcataag aaacgcgtta gttgttgcca
    10501 tcgggataac cgcgatcggt gcattcgtcg gtgctggtgg tcttggagat atcattatcc
    10561 gaggaaccaa tgcaacagag ggcacatcga ttattttagc aggtgcattg ccaactgcat
    10621 taatggcgat catcacggac tggcttttag gtattttgga aagacggcta gatccagcca
    10681 gccgtacatc acgctgatgt tattgtaaaa aacaaaagct tctgcgacca tttgttggtc
    10741 gcaaaggctt ttttagttct gtcattttta aggcaataat ccactctgat tcttccctcc
    10801 ttatgggtaa agagttgctt ttcttagcta tttatccatt ccgtatactc caacttcata
    10861 ctgccaacgg gttcatatct gcatccaccg actaagtttt tttcttatcc gctaaggcat
    10921 tttgccaatc cattttccca tcactcgtca tggaattcat accacatgat ctctaaatac
    10981 tcgtctgctt gttctttgaa ttgggcatca attcttacat cctttggcgt ggctttcatt
    11041 taagcctttt ccatcagtcc tcgattgttc gatgccttca gctgcatcat caaaactggt
    11101 gactgccgta tcggtaactt cgatcatctt ttatacaatc gggaacttgc catcctcatc
    11161 cttggctaaa aacatatcta gccattccag cttctcgcca tgggaggttt gcggttccta
    11221 aaaccggtac acgattctta tggactgcat ccgtcgcaac tagacttggc gatacgatga
    11281 tccttcacca gaagagcacc cccgtaaacc aattgatcga tatattgcca ataagaaaag
    11341 acattggcgt aaaagatatc gatccctcgt gatgcattgc cgttgatact gctattcatg
    11401 gccgaaagcg ccacgacttt aacttcttcc gtttgagtga cattcatgtt cggcaatgtc
    11461 gctttggata cccgctctgc taaaggaacg acactggtat tgtatttcgt atctgtatct
    11521 tttccaaaag accatgccaa cgaatcctct gggaaccaat aagacgactc cggttgattg
    11581 tccattgctt ttttcaacat acttttggct gtttctcgat aaacgacatt tccaattgtc
    11641 ttcgcctcac tttggcgata atcgatggtc gttgccactg ccaacactaa caacgatgct
    11701 ccaattttac tgattttcct tgtcacactt cttcctcctt gttttacata aacccgtcaa
    11761 cattccattc ctaaactcaa acaatgaccc ttcaaaggtg gagatatagg aacaaattaa
    11821 agaaatcatg caataaatcc aagaaggttg gttttagaac aaaaaaagag tctccttttt
    11881 ggggagactc tttgttaact atctaaaaag gttggctctt ctcacttcac tcataatgtg
    11941 tccaggcaga ataaatttct acaactttat tttcctcatc aactttgtaa accactcgat
    12001 gctgtctatt cagcctgcgt gagtatctgc cttcatgtgt aggtctcagc ttttcaaaag
    12061 actgcgtggg catatatgga tcttccttca atgtctgaat cacttcttca aattgtttct
    12121 ttaaattcgt ttgcttgatt tttcttaaat ccactttcgc agagtttttg attgcgactg
    12181 tatagttact catcaaagag cttcccaatc aatatcatcg atattcgtaa agctactgtc
    12241 gtctttttcg cggtctcgta ctttatctaa agtacctgtt tgctcaagaa aaagggtttc
    12301 ttgaatcgct ctccagtctt ccagcccaat caatacagca ttgtttccac tacgatcact
    12361 aataatctcg atttcagtat gattttcatt tacctctttc aataactgat aaaaattttt
    12421 acgagcagtg ctaggattca tcgttgtatt agccattgtc tctcctcctt tttctgatat
    12481 aattatagcg tacttgatta agtacgtcaa aaaaatacaa taaagcactt aaattcagca
    12541 aacttttcct cttttttaca tagagaaaaa gacccctttt tagggaggat ctttgtccac
    12601 gctcacttct tactccttta cgactagcgg tagccaaacg taaagtaatt ggtaacaacg
    12661 tatcatcttt agcaaaggtc gaatccaaag cctgtgagtc tatgcaacaa caccaataca
    12721 cgaaggttcc tcgttgcagt gtgacttgat tgatatcttg aataacctga ggatacgcac
    12781 gaaagatttg taacggttat tcaaaactga ttttggccgt caagtcttct actgcctcaa
    12841 aaatgattgc ctctttcgtc tcctgtcatt catgttcact atcgatttga tacttgctta
    12901 gaccgttggg tttgtgaatc accaattaaa aatggcactg agttatggta acaaaaaatg
    12961 gtcacttgac gaggctgaga ttcctactta tgaagacaca tggcacttaa gagtcgaggt
    13021 aaaggaagaa gctgaaaata taggaggctg tacattattt ggcaaaggat tcggaaatta
    13081 tgatcaagac atcaaaatca aaatgtttta cagctaaata ggaagaataa taacatttca
    13141 tacgctgttc ccatctttaa tggcttatag agggcacctt tagctgagaa taagacaaaa
    13201 cagaccctct agcttcaatt aagagctaga gggtctgttt ttgattttaa aacttggttt
    13261 atttcttcaa atggtagcta taggtgctga caacgatatt ttcacgccaa gacaatcgca
    13321 atcttaaccg caaacttgtt tggttatgga gaatattctg ccagcgtctg tttggtacaa
    13381 attgaggaat caacacagta gttgtatagt tttgtttcgc tgcattttta cttacgatat
    13441 ctacataacg gataattgga ttttcaatcg agcgataaga agagtggacg attgaaaaac
    13501 ggacatctgg gaaatgcttt ttaaattctg cttcgatttc tttttctttt tcgacatctt
    13561 cgtctagcga aacatgcatc gcaacgacat aatcaccgat cgaacgtgcg taattcaacg
    13621 caccgatatt tacccgtgtc acattcccga ctaatacaag aacggtattc ccgtcataat
    13681 catgtagttt tgcatcattt tctaaacgaa gctgttctgc tactttttta taatgatcat
    13741 ggatcttata aaaaatgaac ataacgattg gcataatgat gaagaaaggc caaatgtccc
    13801 ctaaccgata gacaaacaaa atcgcaatga ttgcatatga gatgaacgca cccgtaatat
    13861 tggcaatcga tttgcttaac catctttttc cttctttttt ccatttgacg accatccctg
    13921 tttgagataa agcaaatgga atgaacacgc cgatagagta taacggaatc aagcgttctg
    13981 ttgagccatg gaaaataaac agcaatacga tcgaacctag cgctagtgtg ataatcccat
    14041 tggaatagcc tagtcgatcc ccacgatctt gatacatgtg aggcatgaat ttatcttttg
    14101 ccagattgta tgcaagaact ggaaaagcag aaaaaccggt gttagcagca actgctaaaa
    14161 tcagcgctgt agcaaattga aggacgtaat acaaaatacc atgtccaaag acagctttac
    14221 caatttgaga caaaacagtg acttcttttt caggaacgat cccataccaa tagttgataa
    14281 atgtgatccc cacgaagaag aaacctaaga tcaatgccat catcgtcaat gtagcagcgg
    14341 cattttttgc tcttggcttc ttaaagaatg gtacagcatt actaatcgcc tccaccccag
    14401 ttaaagagga agaaccagaa gaaaatgctc gtaaaatcaa agcgatagaa acacccggca
    14461 cagccgctcc tggaagtgca gtagcttgca atggcacagc acctgtcacg atattatata
    14521 aaccaacaac gatcagtaac gagatcacaa ggataaatgt gtacaccgga aataatagga
    14581 acgatgcaga ttccctcagc ccacgaagat tcagcatcat cagtagcaaa acgattgtca
    14641 cagaaatagc gacttgatga ccgtagagtg ctggcaccgc tgatgtgatc gcttcagcac
    14701 ctgctgagac agatactgct actgtcaaca tgtagtcgat caataaagag ccaccggaaa
    14761 tcaagccagc attctttcca agattctcac tgctgacgac atatgctccc ccgccatgag
    14821 gataagcatg gatgatttga cgatatgata gcgttaatga aatcaataaa ataataacaa
    14881 aagcggcaat gggtagcgaa taccagatgg ctgcagcaga caaagcgact aaaacgacaa
    14941 cgatttgttc cgtaccatat gcaatagatg ataacgcatc agacgacaat aatgccaaag
    15001 cagcaaatcg ggttaatttg tgctcatcat tttcagcaga ttttaaaggc ttacctacta
    15061 acaacctttt taagtaatcc aatttctaaa ctcctttaca cacaatttta cttcaagttt
    15121 tatagtcgaa aatttatagt aaacaagatg ttatagtacg ctcactgacc ttaaaagtca
    15181 ataaacttta agaaaataat tggaaaaaaa cagaaaaata tcaagcgctt tttcaaacgc
    15241 tttaatacag caaaaatcta acattttttt atgggctttt tctttgaatt ctataataaa
    15301 aggatagaac ttgacttaga aaatcaagtt ctatcctttt tgatatctta acttttggaa
    15361 gagagatgta aggctgcttt ctacccatca ttcttactac tcgaagcata gcgcagttgt
    15421 cacaacctct tgataaacgg tgtttaaaag cttgcatctg cggtaataag cccaaccaaa
    15481 ttaaaaacat aagaagctgt ttttatttgg tcgttcttat tacttgatgc agagcagctt
    15541 ttttacaacc tcttggtaca cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa
    15601 acggcaaaat atgaggaact atttttccat ttttttctct aatactcagg tcaaaacact
    15661 gatttcacaa cctctttttt taatgaaaaa tatggctctc atgcgccgct tctttgaatt
    15721 tcgtcatttt ttcttcgatg aacggataga ttgccgttcc tacgattcca aagacaacaa
    15781 acaaaccgat catgatccag attgctttcg tagcatttgg ccaataaatt cctcctgcag
    15841 attcccgtaa caagtttacc gcatgagtaa atggcaagaa cggattgatc atttggaaga
    15901 atttccctga gacttggatc ggatagttcc cgcctccgcc agagatcgac agtactaaga
    15961 tgataatagc gatcccttta ccgacattat cgaataatgc agccaagaca tagacgatca
    16021 tcatgaacgc taaggcgatc aagacagcaa atgacacgct gtagaccgga ttttggacat
    16081 ctactcctag taagaaaata ttccctaatg taacgatcaa gctttgcgca atcgccattg
    16141 tcaaaaatgt cagcatacga gcaacgaatt gttctcgttt cgtaaaattg tttcggtctt
    16201 tctttcctaa atagtattct gtcgtagcaa cacttgaaag aagaacagcg ccgacccaca
    16261 gacatagtgc agtataaaac ggggtgctag ctgaaccgtt atttgcgatt ggataaagcg
    16321 cattggtttg tagctcaaca ggttgtgtaa agaagtcact ttcttttgtt gcgtctagtt
    16381 tcaataattt gatgacttcg cctagatcga cttcttgttc gccttttcgg atcgcgtctg
    16441 ctgctttttg gataccagct ttgatatttg gccagtcatt gttgatcaag tccgcagcag
    16501 cgttcacagc tgtttcgact tctggcattt tttcatttac tgttgtcaat gtaccggtga
    16561 tttcattttt tagtgctggc caatcatttt ggataaaatc agcggccaat cctaattttt
    16621 gctgaatgat cggtaattca ttgttgtata ggtctgcccc ttcattgatg ccattgacga
    16681 tggtatccat atttccattc agcattgtat tggcatcatg gatttcttgt ccaattgcag
    16741 gcatttcttt ttgatatttt tctaaaattg ccactgcatt cgttacagtt gctttcgttg
    16801 agctgagaag cgaatcaaag tcgatctggt ccgctttgct cagtgcgcct tgtgcagtcc
    16861 caatcgtatc gatgacttgg tctagtagag aagtgacagt ggaactgatt tgacctgtat
    16921 tgataccagc aatgatgcca ttgatattat taacaacggc atcaatttga tctagcaatt
    16981 catcaatagc cttcgtgtct tcattttgaa cagctgtgtt caaagcagat aggcggtttt
    17041 gcagattgct tagcaagggt tttaattgat ttagctgatc aataattggc tgtaatttcg
    17101 tatttccagc acttgtttgc aatttagtca ggaactcgac cagtttatcg attgcatttt
    17161 gttgttgaga tattccgtca ataaagccgt tcaatacatt gctgatgttt ttcttttcat
    17221 catcagtcag cttaccatct tcaatagctg ttcttacagt atctattaac gaacctgtcg
    17281 ttgttgccac ttgtccaatt gacttcaatg taacatctac actgtttgtg atgcttggta
    17341 tcgcatcttt cagctgagag gcacctgatt ttgtttggtt tgctagatca cttgcctgat
    17401 tttctaaatt tttcacatct ggcaagatat cttgtacttg ttggatgatc gtcaagcctt
    17461 gttttgcttc atcgattcct tggttcattg tctcttggat cgaagcaaaa tcgccgtcga
    17521 tctctgctag ttgcttaccg gcattttgga tctctgggat tttttcttgc aaatcaagga
    17581 tgatttttgc ttgatctttc aactcaggca ttttgtcatt caatgcaacg actttttcac
    17641 ccatctcatc tactttaggg atgtaatcca cgaattcatt tgctttattc agtttttcct
    17701 tgatttcagg tagttgactt tgtaattcga ggacttgttt tgtgtagccg tcgatcgtgt
    17761 ccaaattttc atctgttgat aaaatcatat ctttaacttt attgatactt actaggttcg
    17821 tatcgatgtc gtaacctatc tcattgaata ctttcaataa agtcgaactg gctgttttga
    17881 taaattcatt tgtgatttgc gattggatcg aacttgctcc tttatcggtg attttcggtg
    17941 caatcgcatt gattttttca tttaccgtat attcgatttt tggtttcttg atatcaccac
    18001 tagtaaaact cagcagatct tctgagaaat cctttggcaa gtaaatacct gcgtaatact
    18061 ttccggaacg gactccgtct tctaattgtt cttttgaatc aacgaattgc catcctaatt
    18121 gtttattttt atgaagcgat tcgatgactt gctccccaat ggccacttct ttaccttgaa
    18181 actctgctgg cttgtctgca ctgtaaacag caattggcag ctctccagta tttccatatg
    18241 gatcccataa tgctttgatg ttaaaccacg catatagcga agggatgatc atgatcgcga
    18301 tgatcagaag tgttgcaatc gggtttttga aaatccgttt ccagtcaagc aaatataatc
    18361 gaagcgtatt tttgattgac tgcatagttc tcccccttct tcttttttca gctgaattac
    18421 tttaccatgt atttcatact tttttcaatc ggcttttttc aaaaaagtcg aatttttgga
    18481 catatcacgg caattgtctc attgtaatct cttacatatt gagaaataat taaggattac
    18541 agcctttac
//
LOCUS       DAZULY010000029        18460 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000029 DAZULY010000000
VERSION     DAZULY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.029"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..485
                     /locus_tag="ACG81A_000682"
     CDS             3..485
                     /locus_tag="ACG81A_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFS5014074.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            522..1532
                     /locus_tag="ACG81A_000683"
     CDS             522..1532
                     /locus_tag="ACG81A_000683"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFS5014075.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1647..2360)
                     /locus_tag="ACG81A_000684"
     CDS             complement(1647..2360)
                     /locus_tag="ACG81A_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014076.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(2533..3140)
                     /locus_tag="ACG81A_000685"
                     /pseudo
     CDS             complement(2533..3140)
                     /locus_tag="ACG81A_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            3624..3851
                     /locus_tag="ACG81A_000686"
     CDS             3624..3851
                     /locus_tag="ACG81A_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFS5014077.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            3867..5033
                     /gene="hemW"
                     /locus_tag="ACG81A_000687"
     CDS             3867..5033
                     /gene="hemW"
                     /locus_tag="ACG81A_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFS5014078.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            5267..6310
                     /gene="hrcA"
                     /locus_tag="ACG81A_000688"
     CDS             5267..6310
                     /gene="hrcA"
                     /locus_tag="ACG81A_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFS5014079.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            6404..6967
                     /gene="grpE"
                     /locus_tag="ACG81A_000689"
     CDS             6404..6967
                     /gene="grpE"
                     /locus_tag="ACG81A_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFS5014080.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7018..8847
                     /gene="dnaK"
                     /locus_tag="ACG81A_000690"
     CDS             7018..8847
                     /gene="dnaK"
                     /locus_tag="ACG81A_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFS5014081.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            8998..10164
                     /gene="dnaJ"
                     /locus_tag="ACG81A_000691"
     CDS             8998..10164
                     /gene="dnaJ"
                     /locus_tag="ACG81A_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFS5014082.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(10246..10875)
                     /locus_tag="ACG81A_000692"
     CDS             complement(10246..10875)
                     /locus_tag="ACG81A_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5014083.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            10887..11060
                     /locus_tag="ACG81A_000693"
     CDS             10887..11060
                     /locus_tag="ACG81A_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014084.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11105..11842)
                     /locus_tag="ACG81A_000694"
     CDS             complement(11105..11842)
                     /locus_tag="ACG81A_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5014085.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(11844..13346)
                     /locus_tag="ACG81A_000695"
     CDS             complement(11844..13346)
                     /locus_tag="ACG81A_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFS5014086.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13605..15599
                     /gene="uvrB"
                     /locus_tag="ACG81A_000696"
     CDS             13605..15599
                     /gene="uvrB"
                     /locus_tag="ACG81A_000696"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFS5014087.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            15609..18425
                     /gene="uvrA"
                     /locus_tag="ACG81A_000697"
     CDS             15609..18425
                     /gene="uvrA"
                     /locus_tag="ACG81A_000697"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFS5014088.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
BASE COUNT         6080 a         3234 c         3963 g         5183 t
ORIGIN      
        1 aaatgattga cttggcaaag aaattcttac acgaaaatat atattttgag ttgtatcatc
       61 atcgagccat ttatacaaat gaagatgctt tggtaataaa gaaagaacaa ggatttacag
      121 gtacagagac caaaagctta ttcttaaaag ataaaaaaga taataactac gtatatctga
      181 cttttactac gaaacaaacg gatttcaaag cattagggaa gatcgtcggt aaaaggcttt
      241 ctattgtttc atcagaacgc atggaagaac aaacgggaca aaagcagggc gctgtttctc
      301 cgtttggtta tgaaaaacag attccgatta ttattgatga agagctctta tcaaatgaaa
      361 aacttgtttt tgcaccaggt agaccagatc aaacgatggt catccaaacg gttgacttaa
      421 acaaaatcat ccagctactt gagatgccga tatttttgat gccggcaaac gaaaaaaaca
      481 attaaccaag taaaatagtg catacttgga ggtgaagcaa catgataagt tatccattat
      541 tagaaagaaa tgcaacgatt ggggttactg caccatcctc aggcgtatca gaggaacacc
      601 attcgttact cgaatcagct ttaaagagaa tggaagaaaa atctttccaa gtagttactg
      661 gaaagacgct ttggacgcaa gataaggaaa aatcaggtcc agcaagaaaa cgagcagatg
      721 aattcaatca aatgatgcaa gatgaaaata tccaaatgat ctttccgact tggggaggag
      781 aattattgat tgagattctc gattttgtcg attttgatgc tctacaacca aaatggatag
      841 taggttattc agatattagt acgttgctac ttgctgtcac attaaaaaca gggatagcca
      901 ctgcacatgg aactaacctt attgatttaa gaggagagta cttggatgaa acgacagcaa
      961 tgtgggaaag tgtactgaaa acaagagaag gagaatcgat tctacagcaa tcttctgaaa
     1021 agtatcaaaa agtgtggccc catgagcatt catcatcttc tgttttccat ctcactgaac
     1081 caacaaattg gcaatcagtc agcggagatg cagtaaggat gaagggacga ttattaggtg
     1141 gttgtattga tacgatcagg catttaatcg gtactcctta tggagatgtc caatctttta
     1201 aaagaaagta taatgatgga aagccgatct tgtggtttct agaaaactgt gagctatcgt
     1261 cagttgattt aagaagatca cttgttcaga tgaaactggc aggctggttt gatggcagct
     1321 cggggatcat gttcggtaga agtgcggcag aggatttgtt agattataaa atgaaggatg
     1381 tatacgaaga tctgtcaaag gaattaaaac taccgatctt gtatgatatt gattgtggtc
     1441 atgtaccgcc acaactgaca ttgattaatg gtgcctatgc agaagtagcg gtagaagata
     1501 gaaaagcgac tgttttgcaa acgtttattt aaaaaatttt ttcctgcctt ggagtctatc
     1561 aaagaccaat aaatctaaat agaaaagaaa gtgaaagaag ctgacaaaat gctgtcagct
     1621 tctttttgta taacatatta gaaagtctaa ttatctataa ctgtcacatc gattaactgt
     1681 ttcacgtcat tgggatttcc tgtaggaata gctactacag atgcattatc aatgatttct
     1741 tgagttgttt ctctaatgaa cgctggataa gatgtttgat ctgcaggcat ttgccaatgt
     1801 gaaggaactg gataactagt gattgtcccg tcatggttac ttgaatacac taaaactccc
     1861 tggtaactgt atttcccagt gagcataacc acatctttcg gatagactgc actccctgca
     1921 tcataagggg cgataggtgt tcctgcagga taatgaataa catgtaattc gaaatcaccc
     1981 gatgtatctc taaaagtatt acctctaata gctagccaaa cacgtgcgta ctcaatttgt
     2041 agatcagaat atcctgctaa cggatctatt gactgagaag actgtgagga ggttgtgtct
     2101 attgttgaag aagaaatatt tgtctcaact gtactactag atgtagaaga agtattgggc
     2161 tctgagactt tactgcttgt tgaagatgta ttcgtttcag tagaagctga tttctgagta
     2221 cttgattttt cagctgaact ggtttgtctg attgaacttt tgtctgtggt cgcagatgga
     2281 tcactatttt ctttactagg agacgtacat cctacaagga ataaagagac caacaataaa
     2341 ataaaaccta actttttcat ataaaatacc tcattatttt tttaatacca gtatagcata
     2401 atcctgaatt tttgaatccg taaaaccttg tctatgaata taaatagagt gtatcttctt
     2461 tgataaatgt cagaaaaagt gtgacaaata atcatcagta aaattacttg tcacactatt
     2521 gataaggagt atttaattga agagaatgat taggagaaag gatctccgta ctttttatag
     2581 taatcataga atccaagttt gtatagacga atcgtttttt ctcttatttc ttctgtttca
     2641 aaatggactg cttgaagaat caagtctgtg aattctatag gagctgttcc attcgcagta
     2701 atcaaattac gatctttcac agcttgttgt tctataaaat cttctttatt ctgataattt
     2761 tccgattcta accaaggaaa ttgcgcattt ccggtgtgtt tgtaaccttg caataatccg
     2821 ctctgagcta aataatcaac tgccccacaa atagcagcta ctggtacgtc tgtttttaac
     2881 aatttatcga ttatttgata taaatgatga ttttttatgt cccatgaatt gccaccgatc
     2941 ataataaaaa ggtcaacttt ttcaggtatt tgttcgatta agtaatcgat ttttgttttt
     3001 aatcctccaa tggaagtgac ctctgattgg atagaagcag ttttgttttg ccatttctca
     3061 ttttggttta gtgtgctagt aatataagca ccttcccaat cggcatattc ctctaataga
     3121 aacgtaatcg cagttttcat tttcaagctc tcctttacta tctataacaa ggacaacagt
     3181 tttaacctac agacattcat taaaattggg tagcgacagc gaaaacaaca attcttaagt
     3241 tttagacaga gtatacagat agtcaactta attaaatacg aactaaataa aaatgacagg
     3301 taatcatttt tgtttaccag cattataaca ttttgcatac atggatgaca aaagaaccaa
     3361 ttcccacttg cttgagtaca atattaaaag aaactggaag gcaaaaagta attatagaaa
     3421 cgaaaaataa atcatatcgg gcactcagac atgaaaacaa ccctagagat atataactag
     3481 gtaaccagtt taatgaaaga aaaaatcatc caagaagtcg attcttggat tttttagtgg
     3541 atttgttcgc caaatacgtt tagtatgcgt atatgagagg ttgtaagcaa agcgttgtgg
     3601 tctgagcact taaggaggaa tctgtgaaaa tagcttttca tatttttgca gattttgact
     3661 taatgccgaa ggtcagcttt gcgattaccg tttatcgaga ggctgtaaaa gcaccgtgta
     3721 gctccgatga caataagcaa gcatttttag agctaggttt ccttgattct aaatatgcgg
     3781 cttatttcaa tgagttggtg cttgaacgcg attttaagaa ggtcgaaaac aggcacgact
     3841 tttcgacgta aggagaaaga atttcaatga tagaaacaat aaatcccgca gaaaacaagg
     3901 tttctgcgta cattcatatc ccattttgcg agcatatctg ctattactgt gattttaata
     3961 aagttttttt agaagggcag ccagtagatg agtatgtgga aatgctgctg aatgaaatga
     4021 aaataatgct agaaagtcat ccgacagcga atttagaaac actttacgta gggggcggga
     4081 caccaacttc tttatctgct aaacaacttg atcgactgtt atcaggtgct agagagatcc
     4141 tgccttttca aaatggtaat gagtttactg tagaagcgaa tccaggtgat ttgacccgtg
     4201 aaaaacttca agtaatgaaa aactatggcg tcaatcgttt atccatggga gtgcagacat
     4261 ttgataatcg cttattgaaa aaaattggac gtaaacacac cgcagaagat gtgtatcaaa
     4321 ctatgcaatt tttagaagca gaaaatttta ccaatgtaag catcgatttg atttatgcat
     4381 taccgggaca aacactagaa ggatatctgg atactttgga aaaagctttg gctttggatc
     4441 tgcctcacta ttctttgtac tcattgatcc tcgaaaacaa aacaatgttc atgaactggg
     4501 tacgacaagg aagactcgaa ctgcctgatc aggaaacgga aaccagaatg tttgaagaga
     4561 cgattgaagc aatggaaaag catggacgtc accaatatga aataagtaac tttgggctct
     4621 ccggacatga atctcaacat aacctcatgt actggaacaa tgaccactat tttgggtttg
     4681 gagctggtgc tagcggttat cttggagata ttcgttaccg caacaaagga ccaatccaac
     4741 actatttacg cccattacga gcaaatgaac gcccggtatt ggaagaagaa aaattgacac
     4801 gaaaaaatca aatagaagaa gaaatgttct taggactaag aaaaaaaaca gggattacta
     4861 aaaaacattt ctatgatcgc tatcaaaaaa caattgaatc ggtgtacggt gatgtgctaa
     4921 gggaattaga gcaagaaggg ttacttgtta atgaagaaga ccgtgtttat ttgacaccaa
     4981 gagggacctt tttggggaac aatgtgtttg agcgcttttt gtttgaaaaa tagtttaaag
     5041 aatatttgtt ttttatctga atttaaataa aaaaagttca aaaatacaat aaaggactat
     5101 tgaaacgttg atataactgg atttacatca attatttaga attagcactc ttgtgtttag
     5161 agtgctaaaa aatggagtct ttctcttgac aggagaaggg cttcttgcta tatttataag
     5221 tggattagca cttagaatat atgagtgcta atgagaggtg aggaagatgt taacaaagcg
     5281 acaaaacgac attttacgtc tgatcatcca acactatacg agtagtggcg tgcccgttgg
     5341 ttccaaaaca ttgatgtctg agggagtcaa ggccagctca gcaacgattc gcaatgatat
     5401 gaaagcatta gaagatgaag gattgctgtt gaagactcac tcttcttctg ggcggattcc
     5461 atctgcttta ggctatcgtt attacgtgga tcatttgtta aaacctgctc gcgtagcaga
     5521 agatgatttg ctgctgattc gtcagtctct aggcagagaa tttcatgaaa tcaatgagat
     5581 tattaagcaa tctgctgaga tattatctga gctgacaagc tatacgactt tttcattggg
     5641 acctgaaata aaagaccgcc gactgactgg ctttcgtatc gtacccttga ataatcgaca
     5701 gattattgct atcgtcgtaa cagatatggg gaatgtagaa agccaagtgt tcactcttcc
     5761 cgaatatatg ggaagtcagg aactagaaaa aatggtacag attgtaaacg accgtctagt
     5821 cggagatcct ctgatgacgg tgtaccataa acttcgtacg gagattccga tgattcttca
     5881 taaatatttc caaacgacag aagggatgtc tcagttattt gacaccgttc ttggtcatgt
     5941 ttttgaagat aaggtttatg tcagtggaca aatgaatctc ttggattatg agccccgtca
     6001 agatcttgat cagttcaaat cgatgttttc ttttatgaaa gactcagacg gacttacaca
     6061 aatgatcact ccgatggata gcaatattca tatcaagatt ggtgctgaat taggcaatga
     6121 tttattgcag aatatgagta tgattcaagc aagttacgag atagatggtc atggaagagg
     6181 tacaatagcc ttgttagggc ctactagtat gccttattct cggatgttcg gtctagtcga
     6241 tgtttatcga agagaactag cagctaaact tgctgattac taccgttcgc ttgacttgtc
     6301 aaactcgtaa tgtgaataac gaagctattt gcagctgatc gttctctctt tcttagtaag
     6361 agcaatcaaa cagctgactg aataatgaaa ggaagactgt atcgtgaaag aaaatcaaga
     6421 agaactagat aaagaaatga cagatgctca gccagaaccg gaaatcgatg tcgaagctgc
     6481 agaagattcg ggtatttcag aagctgaagc ggaagaattc gaaacagcga aattaaaagc
     6541 tgagttagaa gaaatggaag ataaatactt gcgggctcgt gctgaaatcg caaatatggc
     6601 aaatcgagga aaaaatgagc gtgagcaatt acaaaaatac cgttcacaag atttagccaa
     6661 aaaattactt ccatcaattg acaacctgga acgtgcatta gccacagaag tgtcagatga
     6721 tcaaggggca ggtttgaaaa aaggcgtgga aatggtcttg gaaagtttgc gtaacgcact
     6781 ggaagaagaa ggaattgaaa aaatccctgc taaaggagaa gcctttgatc ctaatcttca
     6841 tcaagcagta caaactgttc cagcgactga agatacacca gccgatacga ttgtagaagt
     6901 actacaagaa ggctacaaat tacatgatcg cgtgttaaga ccaaccatgg tcattgttgc
     6961 acaataattg tgcggatata aaacttacac tttcaattaa agaaggagac gttaattatg
     7021 agtaaaatta tcggtattga cttaggtacg actaactctg cagtagcagt attggaagga
     7081 aacgaagcaa aaatcatcgc taacccagaa ggaaaccgta caacaccttc tgttgtatca
     7141 ttcaaaaatg gagaaatcca agtaggggaa gttgcaaaac gtcaagctgt gacaaaccca
     7201 aatacgattt catcaatcaa acgccatatc ggtgaagcag gttacaaagt agatgtagaa
     7261 ggtaaatcat atacaccaca agaaatctca gcaatgatcc ttcaatatat caaaggattc
     7321 gcagaagact atttaggtga aaaagtcgaa aaagcagtta tcactgtgcc agcttacttt
     7381 aatgatgcac aacgtcaagc aacaaaagat gctggtaaga tcgctggtct agaagtagaa
     7441 cgtatcgtca atgaaccaac tgcagctgct cttgcctatg gtttagataa aacagatcgc
     7501 gacgaaaaaa tcttagtatt cgaccttggt ggcggtactt ttgacgtatc tatccttgaa
     7561 ctaggagacg gtgtctttga tgtattgtca acagctggcg acaaccatct aggtggggac
     7621 gactttgata acaaaatcat cgactacatg gtagcagaat tcaaaaaaga aaacagcatt
     7681 gacttgtctc aagacaaaat ggctttacaa cgcttgaaag acgctgctga aaaagcgaaa
     7741 aaagatttat ctggcgtatc aagcacacaa atcagcttgc catttatcac agcaggagaa
     7801 tctggaccat tgcatttgga aatgacattg acacgtgcaa aatttgatga attaacagct
     7861 gacttagtag aacgtacaaa agtaccagta cgtcaagcgc taaaagatgc aggtttatca
     7921 caatcagaaa tcgacgaagt tattttagtc ggtggatcta ctcgtatccc tgcagttgta
     7981 gaagcagtac gtaaagaaac aggaaaagaa ccaaacaaat cagttaaccc tgatgaagta
     8041 gttgctatgg gtgctgctat ccaaggtggc gtgatcactg gtgatgtgaa agatgttgtt
     8101 ttacttgacg ttacaccatt gtcattaggt atcgaaacaa tgggtggagt gttcactaaa
     8161 ttgattgacc gcaatacaac gattccaaca agtaaatcac aagtattctc aactgcggca
     8221 gataatcaac cagcagtaga catccatgta ttacaaggtg aacgcccaat ggctgctgac
     8281 aacaaaactc taggaagatt ccaattgaca gatattccag cagctcctcg tggtattcca
     8341 caaatcgaag taacattcga tattgataaa aacgggattg taaatgtatc tgcgaaagat
     8401 ctaggtacac aaaaagaaca aaaaatcacg atcaaatctt cttcaggtct aacagacgaa
     8461 gaaatcgaac gtatggtaaa agatgcagaa gcaaatgcag aagcagataa agctcgtaaa
     8521 gaggaagttg acctacgtaa tgatgtagat gcattgctat tctctgtgga caaaacattg
     8581 aaagaattag aaggcaaagt ggacgaagaa gaagtgaaaa aagcagaaac agctcgcgat
     8641 gaattgaaag ccgctgttga agcaaacaac atcgaagaaa tgaaaactaa acgcgatgca
     8701 ttgaacgaaa tcgttcaaaa cttgactgtt aaactatacg aacaagcggc acaacaacaa
     8761 gctcaagaaa acccagaagc agctcaaggc ggagcagatg atgtcgttga tgctgatttt
     8821 gaagaagtcg atggcgacga taaataatca gttcactgaa tttcgagaga aggccaaagt
     8881 tgctactttg gctttttctt ttgaaaaata gtttgaagta tcggtttttg tttcaaacaa
     8941 aatatgatat gataacaagc gacgtgaaaa tgatgagaac caataggagg aaagcctatg
     9001 gcaacaaaac gtgattatta tgaagtctta gggctctcaa aaggagcatc agaagatgaa
     9061 ataaagaaag cttatcgtaa gctttcgaaa aaataccatc ctgacatcaa taaagaacct
     9121 gatgcggaag aaaaattcaa agaagtatca gaagcatatg agattttaag tgacccgcaa
     9181 aaacgcgctg catatgacca atacgggcat gcaggcacag atccaaatta cggtgctggt
     9241 ggagctggtg gcggatttgg tggattcggc ggcttctctg gtggcggctt cggtggtttt
     9301 gaagatatct tcgactcttt ctttggagga ggcggtcggt cagtcgatcc aaatgcgcct
     9361 cgtcaaggtg ctgatttgca atatacggtc gacttgtctt ttgaagaagc gatttttggt
     9421 gtagaaaaag aaatcaagta taatcgggaa gaaatctgtc atacttgtgg tgggaacggt
     9481 gcaaaaccag gcacacaacc aactacttgt cataaatgtc atggtgcagg tacgattaat
     9541 gtagaacgcc aaacaccact tggacgagtg atgagccgtc aaacttgtga tgtttgtcat
     9601 gggacaggga aagaaatcaa agaaccttgt ccaacctgtc atggctcagg acatgagaag
     9661 aaagcacatt cagttaaagt gaatgtacca gctggtgtag aagaaggaca acaaatgcgt
     9721 ttagctggtc aaggagaagc tggagaaaac ggcggaccat ttggtgattt gtatgtggtc
     9781 ttccgtgtag aagaaagtga tatctttgat cgtgaaggtt ctgaaattta ctatgaattg
     9841 cctttaagtt ttgtacaagc agctttgggg gatgaagtcc aagtaccaac tgtccatgga
     9901 gatgtgaaat tgaagatccc tgctggaaca cagacaggta cgaacttccg tttacgtgga
     9961 aaaggcgctc cgaagttacg tggtggtgca acaggcgacc aacatgtcaa agtaaaactg
    10021 atcacaccga aaaatctaaa tgaacaacaa agagaagcat tacgtgcatt cgctgaagct
    10081 ggcggcatca aagttgaaga acagcaagaa gacggtttct tcgataagtt taaagatgct
    10141 tttggcggga aaaagcggaa gtagtttttc tagtaaaaac gctaagtcat taataattaa
    10201 aaaccagacg aacaatcata tgttcgcctg gtttttttgt agttgtcatg tttgtaccaa
    10261 caaaaaagcc agctttagaa tatgataatc gtcatagttt ctaggggtat aaccagtgag
    10321 agaatggaac ttgttcaatc gatattgcaa ggtgttttta tgaataaata attcttctga
    10381 gcaatttttg atgcttccgt tatttttact gaagcatgcc aaagtcttct tgaacaaatg
    10441 tacttcttct tctgtcaggc ttttcaaaat acgttttaca tataatgttc gttgatctga
    10501 cgggattgct ggtaaaacga tcccgaggtc taaatcttca aaaaagcgga tctcgttcac
    10561 agattgatgg aaagctatcc attcgcaaac ttgtttggca tgaaggaaag attgcggtgt
    10621 ctcttgctct gtataacaaa gcgacccaat tccaaagttt agctgtaact gatacatttt
    10681 attaaacttt gtctggattt tatcaagcaa tgatagcaat gaggctctgg aaatccgatt
    10741 gactaggata atgatttgtt gtctatgtac agtgagcaaa gcctgtccgt aataagagaa
    10801 gaccctttcg agcaactcca ataaaggatt gatccgttct tgacacaatt gctgattgtc
    10861 agaaagttgt cccacaacta cataatatgg agaagaaaaa tcaagctcaa ttaagtgagg
    10921 gtggtatcga ctatgtactt gatatcatca atgaaccgat ggtactttct tatgcgatgg
    10981 aacaggtaga gcgattgctt gtttgtgcag gaaaacaagc gatgcggctg atacgattag
    11041 gtgagaaaat aagaagataa aaatacacgg tcccttcatg gcgaccgtgc atttttggtt
    11101 tttattaaat atttaatact ttattcaaga aatcttttgt ccgttcattt tgcggatttt
    11161 caaagatttg ttgaggcgtt ccatcttcta aaaagtttcc gtcgtcaatg aacatgacgc
    11221 gattggccac ttctttagcg aaccccattt cgtgcgtaac gataaccatc gtcatacctt
    11281 gttctgctaa ctttttcatg acgttcaaga catcaccgac catttcagga tcgagtgcag
    11341 aagtaggctc atcaaaaagc atgatgtcag ggttcatcgc tagagcacga gcaatagcca
    11401 cacgctgttt ttgtccacca gaaagcattt caggatacat atctttttta tcttctagac
    11461 caactgtctg cagtaatttg attgcttttt tctctgcttc ttctctagat tctttcttca
    11521 ggtccatcgg tgctaaaatg atgttttcca aaatagaaag atgagggaat aggttaaagt
    11581 gttggaatac cattccgata tgttgtctca ccaagttgat atctgtgctt ttatccatca
    11641 agtgggcgcc gtcaataata atttcaccgt cagaaggttc ttctaaacga ttcaagcaac
    11701 gcaagaatgt actttttcct gaacctgaag ggccaatgat acagacgaca tctccttcgt
    11761 tgatggatac attgatgtcg tttaatactg tgttatcgcc gtattttttg actaaatgct
    11821 caactaagat tttttctgcc atcttacttc accttctttt ctaaaacttt tgctaatttt
    11881 gtcaatgctg tgatcaaaat caagtacatg atcgcaatga tcaagtaaac atacgtactt
    11941 tgtgttgtac gtgcaacaat gatcttacca gtttgcagca attcgactac accgattgcg
    12001 gaaatgatcg ttgtgtcttt taatgagata acgaactgat tgacgaatga tgggatcatg
    12061 atgcgtatcg cttgcggcaa aatgattttt tgcattgtcc gattataaga aagacctaaa
    12121 cttctggaag cttccatttg tccgacagga acggcgttga tcccgcctcg aacaatctct
    12181 gcgatataag cacttgcgtt aagagtcaag gtgataacac cagcaaggaa gtcaggaatc
    12241 gtaaatccaa cgataccagg caacccaaag aaaataaaga acgcaagaac catcattgga
    12301 attcctcgga tgatatcgac ataaatacta gcaattgtgc gaagagcgcg tattggtgct
    12361 acactgaata atccaaaaat cacaccgatt actatagcca aagcaaagga gatcaaagtt
    12421 aggagaattg ttctccaaag tcctttcaac aatgcttgat agttgttttg gataagtcca
    12481 agaaatgttg attcatcagc agtggaagac tcactggaag tttctgaacc atccgctaca
    12541 tattctttaa taatcttatc gtattcgcct gttcttttta attctttcaa tccttcattg
    12601 aacatattaa gaagctcagg attttgtcct tttttaacgg caaatgcata ttttccgcca
    12661 gattcttttt tgatcggtgt ttccaattct tgtccttgtt tgatcgcata accgattacc
    12721 ggataatcat ccatcattgc atcgattgcg ccaactttta acccgtcgta caattgatca
    12781 gcagcatcaa aatatttgat agaatagcca tattcagatt catggtcgct taaaaattca
    12841 gcactttcgg taccaacttt tgctccgact gtttttcctt tcaaatcttc ataagacttg
    12901 atcgaagtat ctcctttttt gacagctaat tggatcccgc tatcaaagta agaatcagaa
    12961 aaatcatagc ttttctttcg ttcatctgtt acagtcattc cggcaatcat tccgtctgct
    13021 tgtcctgatt ccaaagcttg caaagcagca gagaaaccac ggtagtccat tcggatgtta
    13081 aatccttgca gtttggcgat gttttccatc aattttacat cgatgccttc gtactcgccc
    13141 tttgcgtttt gaaattcaaa tggagcaaaa gcagagtcac tggcaattac gtatacgtct
    13201 tttttgggct ctattttttg cattgtctcg cttgtgcttg actctgccgt tgtagtgttg
    13261 tcagcttgtg caacatgacc aaacgcaaat gtcacaagtc ctgtcattaa gaaaacaaat
    13321 gagtgaaata aatgctttct tttcataatc aacctcctaa atttccaata ttttattatt
    13381 ataggcgaaa agtgctgaat cagacaagac tgtaagctcg aaaaaatgaa aaaaatgcga
    13441 gtttctaaca ttttatgtta taaaacataa catgtataat aagataaaaa agtggcgaaa
    13501 gtatgttcgg tgtggtataa ttttttagta aggaatgcct ttgctttcct tttcttttta
    13561 ttggtcgtaa aatgaaaagg aatgaatgaa aaggaggacg aagaatgatt gagagagata
    13621 cctcccgaca ctttgagctt gtctcgaaat accaaccggc tggtgatcag cctgaagcga
    13681 ttaaccaatt agtggatggc gtagtcggcg ggaaaaaagc acaaatctta ttaggtgcga
    13741 ctggaacagg gaaaacatat acgatttcca atcttattga gaaagtgaat aaaccaacac
    13801 tgattattgc ccataacaaa acattggctg gtcagctgta cggggaattc aaggaatttt
    13861 tccctaacaa tgctgttgaa tattttgttt cttattatga ttattatcag cctgaagctt
    13921 atgtgccttc aagcgatacg tatatcgaaa aagattcaag tgtaaatgat gagatcgaca
    13981 aattacgaca ttcggctact agttcattat tagaaagaaa tgatgtgatc gtcattgcat
    14041 ccgtttcatg tatttttgga ttaggctcgc cttttgagta ccaaaaacaa gtagtttcga
    14101 ttcgacaggg agcagaacta gatcgcaatc agttgattcg tgacttagtc agtatccaat
    14161 tcgaaagaaa tgatatagat tttcagcgcg gtcgttttcg agtgcgcggt gatgtcgtgg
    14221 agatttttcc ggcttcaaga gatgaacgtg ccttacgtgt ggagtttttt ggagatgagg
    14281 tcgaacgtat ccgagaagtc aatgcactga caggtgaagt tttaggagaa acggaacacg
    14341 tagcaatctt cccggcgact cactttgtga cgaatgatga acatatggag catgccgtag
    14401 cgaatatcaa agcggaatta gaacagcgtt taactgtctt gcgaaatgaa aacaaacttc
    14461 tggaagctca acgtttagaa cagcgaacca attacgatat tgaaatgatg ttagaaatgg
    14521 gctacacgtc gggaattgaa aattattctc ggcatatgga cggacgtaaa gaaggtgaac
    14581 cgccttatac gctattggat ttctttcctg aagatttttt gatcgtggcg gacgaatcgc
    14641 acgtaaccat gcctcaaatc agaggaatgt ataatggtga ccgagcgaga aaacagatgc
    14701 tagtagatta cggtttccgt ttgccatctg ctttagacaa tcggccactt cggttagaag
    14761 agtttgaaaa acatgtcaat cagatcattt atgtctctgc gacgccagga ccatatgaac
    14821 atgaacaaac agataccgtt atccaacaaa tcatccgtcc gacagggctg cttgacccag
    14881 tgatcgaagt tcgtccaatc atgggacaaa tcgatgatct agtaggcgaa ataaatgaac
    14941 gagtcgaaaa agaccagcgg gtgtttgtaa cgacattgac gaaaaaaatg gcagaagacc
    15001 tcacagatta tttcaaagaa ttagggatca aagtcaaata tttgcatagt gatatcaaaa
    15061 cgctggaacg aacagaaatc attcgagatc ttcgattagg tgaatttgat gtgttagttg
    15121 gaatcaattt gcttcgtgaa ggattagacg tacctgaagt ttcccttgtt gctatcttgg
    15181 atgcggataa agaaggcttt ttacgtagtg aacgttcctt agtccaaacg attggacgag
    15241 ctgctcggaa tgaagaaggt aaagtaatca tgtacgcaga caaagtaaca gattctatgc
    15301 gtctagcgat ggatgaaacg tcaagacgtc gtacgattca gcagaagtac aatgaagaac
    15361 atgggatcgt tccaaaaacg atcattaaag aaatccgaga tttgatttct attacgaaag
    15421 aatcggaaga tgatacaaaa gaagcggtac aagtctcgta cgaagaaatg acgaaggaag
    15481 aaaaagacac gttattgatg aaattagaaa aagaaatgaa agatgcggca aaagctttgg
    15541 attttgaaac agccgcgaat gtgcgagata tgattttaga attaaaagct tcaaagtagg
    15601 aggacaacat ggctaacgat aaaattacga ttcatggtgc acgtgcccat aacttgaaaa
    15661 atatagatgt aacgattcca cgagataaat tcgtggtcgt tacaggtttg tccggttctg
    15721 gaaaaagttc cctagcattt gatacgctgt acgctgaagg gcagcggaga tacgtagaga
    15781 gcctttctgc ttatgctcgt cagtttttag gacaaatgga taagccggac gtagatagca
    15841 tcgacggatt gagtccggcg atttctattg atcagaaaac aacaagtaaa aatcctcggt
    15901 cgacagtagg tactgtgacg gaaatcaatg actatttacg cttgttgttt gcacgtgtag
    15961 gacatccgat ttgtcccaac gatcatatcg agattaccag tcaatcagta gatcaaatgg
    16021 cagatcaagt aatggctttg cctgagcgca caaagatcca agtgttggca ccgatcgtgg
    16081 taaagaaaaa aggacaacat aaaaaaatat tcgaacgaat ccaaaaagag ggatacgtcc
    16141 gcgttcgagt cgatggtgag atctatgatt taagtgaagt gcctgagctg gagaaaaaca
    16201 aaaaacatga tatcgcgatc gtcatcgacc ggatcattgt gaaagaaggg attcgttctc
    16261 gcctatttga ttctttagag gcggccctgc gattagcaga cggctatgct ctggttgacg
    16321 tgatcggtga agaagaaatg ttgtttagcg aacactatgc ctgtccttat tgcggattta
    16381 ctgttggcga actagaacca agactgttct cattcaatgc gccttttgga gcttgtccag
    16441 actgtgatgg tttaggtgtc aaattagaag tcgatcaaga tttagtgatt ccagatagaa
    16501 cgaagacact tagtcaaggt gctatcgttc catggaatcc aatcagctct cagtattatc
    16561 ctcaaatgtt agcgcaggct tgtgaaagtt tcggtatcga tttagataca ccattcgaag
    16621 aattaccgga agagcatcag gagatcatct tgaatgggac agatgatctt ttccatttcc
    16681 attatgaaaa tgattttggt ggcgttcgcg atgtagaaac gactttcgaa ggggtattga
    16741 agaatatcga gcgtcgttat catgatacga acagtgattt cacgcgtgag caaatgcgtc
    16801 tttatatgac agaattaact tgtcagacat gtaaaggtta tcgattgaat ccccaagctc
    16861 tatctgttca aatcaatgga acgaatatcg ggcaaacaaa tgagttgtct attcaaaaag
    16921 cagtagattt cttctcaaac ttaacgttat ctgaacaaga gaaagtaatt gccaaaccga
    16981 ttttaaaaga agtaaatgac agactttcat tcttggaaaa tgtgggacta gactatctaa
    17041 ctttgagtcg cgcttccgga acattatctg ggggagaagc gcagcgaatc agattggcta
    17101 cacaaattgg atcgaatctt tctggagttt tgtatatttt agatgaacca tcgatcggat
    17161 tgcatcaacg cgacaacgat cgactgattg gctcattaaa gaaaatgcgt gatcttggca
    17221 acaccctcgt tgtagtcgaa catgatgaag atacgatgtg ggcagcagat tatttgattg
    17281 acgtcggacc tggggccgga gaacaaggcg gacaaatcgt ggctgcaggc actcctgaag
    17341 aagtagctaa tgatgaaaat tctttgacag ggaaatatct ttcagggaaa aaatcaatcc
    17401 ctgtaccaaa agaacgccgt aaaggtaatg ggaaagcaat caagatcact ggtgcttcag
    17461 aaaacaatct gaaaaatatt agtgttgaat ttccattagg agaatttgta gcagtcacag
    17521 gtgtgtctgg ttctggaaag agtacattgg tcaatagtat cttaaagaaa tcgttagcgc
    17581 aaaaattaaa taagaattct gctaagccag gtaaattcaa gacaatttcc ggctacgaaa
    17641 gtatcgaaaa gatcatcgat atcgatcaaa gcccaatcgg ccggacgccg agaagtaatc
    17701 cagcgactta tacaagtgta tttgatgata tccgtgggtt atttgctcaa acgaacgagg
    17761 caaaaatgcg gggttataag aaagggcgtt ttagtttcaa cgtaaaaggc ggtcgttgtg
    17821 aagcttgtcg cggggatgga attattaaga tcgaaatgca ctttttgcct gatgtctatg
    17881 ttccttgtga agtatgtcat ggtaaacgat ataattctga aacattagaa gtgcattaca
    17941 aaggaaaaag cattgctgat attttggaaa tgacagtaga ggatgctgta gaattcttca
    18001 agcacattcc aaagattcat cgcaaactgc aaacgattgt tgatgttggc ttaggttatg
    18061 tgacgatggg gcaaccagca acgacattgt ccggtggtga ggcacaacgg atgaaacttg
    18121 ccagtgaatt gcacaaaatc tctaatggaa agaatttcta tatactagat gaaccaacga
    18181 caggacttca tagcgatgac atcgcccgct tgttgcatgt attacaaaga ttagtagatg
    18241 ctggtaacac agttttagtg attgaacaca atctagatgt aatcaaaaca gcagattata
    18301 tcattgattt aggaccagaa ggtggagaag gtggaggaac gatccttgcg actggaacac
    18361 cagaagaaat cattaacgta aaagaaagtt atacaggtca ctatttgaaa aaaataatgg
    18421 tataaagagg atagatagct gagatagtta aaaaaacata
//
LOCUS       DAZULY010000030        18346 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000030 DAZULY010000000
VERSION     DAZULY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.030"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..566)
                     /locus_tag="ACG81A_000698"
     CDS             complement(111..566)
                     /locus_tag="ACG81A_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014089.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(720..1928)
                     /locus_tag="ACG81A_000699"
     CDS             complement(720..1928)
                     /locus_tag="ACG81A_000699"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFS5014090.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2040..2372)
                     /locus_tag="ACG81A_000700"
     CDS             complement(2040..2372)
                     /locus_tag="ACG81A_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS5014091.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2520..3395
                     /locus_tag="ACG81A_000701"
     CDS             2520..3395
                     /locus_tag="ACG81A_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5014092.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3477..4058)
                     /locus_tag="ACG81A_000702"
     CDS             complement(3477..4058)
                     /locus_tag="ACG81A_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFS5014093.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4064..5323)
                     /locus_tag="ACG81A_000703"
     CDS             complement(4064..5323)
                     /locus_tag="ACG81A_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS5014094.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5325..6371)
                     /locus_tag="ACG81A_000704"
     CDS             complement(5325..6371)
                     /locus_tag="ACG81A_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014095.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6606..6881)
                     /locus_tag="ACG81A_000705"
     CDS             complement(6606..6881)
                     /locus_tag="ACG81A_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFS5014096.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7124..8434)
                     /gene="der"
                     /locus_tag="ACG81A_000706"
     CDS             complement(7124..8434)
                     /gene="der"
                     /locus_tag="ACG81A_000706"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFS5014097.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="ACG81A_000707"
     CDS             complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="ACG81A_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFS5014098.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9980..10660)
                     /gene="cmk"
                     /locus_tag="ACG81A_000708"
     CDS             complement(9980..10660)
                     /gene="cmk"
                     /locus_tag="ACG81A_000708"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFS5014099.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10709..11344)
                     /locus_tag="ACG81A_000709"
     CDS             complement(10709..11344)
                     /locus_tag="ACG81A_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFS5014100.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11410..12813)
                     /locus_tag="ACG81A_000710"
     CDS             complement(11410..12813)
                     /locus_tag="ACG81A_000710"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFS5014101.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12800..13831)
                     /locus_tag="ACG81A_000711"
     CDS             complement(12800..13831)
                     /locus_tag="ACG81A_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5014102.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13862..14083
                     /locus_tag="ACG81A_000712"
     CDS             13862..14083
                     /locus_tag="ACG81A_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFS5014103.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14248..14985)
                     /locus_tag="ACG81A_000713"
     CDS             complement(14248..14985)
                     /locus_tag="ACG81A_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFS5014104.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15138..16478)
                     /locus_tag="ACG81A_000714"
     CDS             complement(15138..16478)
                     /locus_tag="ACG81A_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS5014105.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16715..18238)
                     /locus_tag="ACG81A_000715"
     CDS             complement(16715..18238)
                     /locus_tag="ACG81A_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS5014106.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT         5178 a         3909 c         3102 g         6157 t
ORIGIN      
        1 aaaaatcttg tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt
       61 agtaataaga cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa
      121 aattccccgt atacatcttc tacacgttga tctggattga tcacgatata caagttacct
      181 gttttttcgc tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct
      241 agatatggga agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg
      301 atatcttcgt atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata
      361 tcatgtgtcg caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga
      421 tggatttcaa caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc
      481 caaagatcgc cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat
      541 tttagcgctg tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg
      601 tactacctca gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa
      661 tgacgtatct gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt
      721 taaatcaatt tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt
      781 tgttctacga cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt
      841 tttccagttt cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata
      901 ggtaaacgat cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc
      961 atcaattctt ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc
     1021 aaagctccag tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt
     1081 tggttagaac acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt
     1141 tggctgatca ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca
     1201 gcaaaacgta aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct
     1261 gtttcaataa atgcatggat tcccgcggcc ccttcatttc ccaagagcat cttgatgaat
     1321 tccacattta tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg
     1381 tagaaggttt cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc
     1441 attcgtaagg catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt
     1501 tcttctagat cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca
     1561 aacgcattga tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg
     1621 acatgatctg gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca
     1681 tattgttcat cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt
     1741 tttggaaaaa gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg
     1801 attggctggt tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct
     1861 gcattctcta attttttcag aactggaatt gccttttggt attccagagg taattgtttt
     1921 aatttcattt ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca
     1981 tggtgactgt ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt
     2041 tactcgtcct tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt
     2101 tcaaaaactt cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa
     2161 acatcaccaa gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct
     2221 ccataagtgt ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc
     2281 tgagctaatg gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc
     2341 tgcatactgt ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa
     2401 aactttaacg caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt
     2461 aggaaggaat ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata
     2521 tggtcaaacg aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg
     2581 catttggtct tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga
     2641 tcaccttgat cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca
     2701 acatcccatt gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg
     2761 ttctaggtac actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa
     2821 tcaatttaca gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag
     2881 gaagcgggat cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga
     2941 ttttagaaca aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg
     3001 tcttacttgc ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt
     3061 cgtatgtttt cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc
     3121 tacttgtcgt ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa
     3181 gaggtgtgac tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct
     3241 atatggtcgt tagctctagt gaattaactg aagctaagag gatcgtccac gagattgacg
     3301 aaaaagcttt cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt
     3361 tgaagccaca gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga
     3421 ggaaagattc cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa
     3481 ttgttatgtt cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta
     3541 acttcatcag ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt
     3601 tgagcataaa gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt
     3661 aattcagtat cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag
     3721 atagatagat attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc
     3781 tttttccaat tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc
     3841 tgcaaaaaca atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga
     3901 tccgcaaaat tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca
     3961 agataattca agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac
     4021 gttaaaaaat tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt
     4081 catctaataa tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata
     4141 attcttgcgc tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga
     4201 aatctggttc atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa
     4261 agtcttctaa ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt
     4321 cttccatctg attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat
     4381 tacttaaagt aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa
     4441 gttcttctgc tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa
     4501 gttcgacttg gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa
     4561 tcatttcttc ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat
     4621 taacttctcg aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat
     4681 tcgtaaaagc tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta
     4741 aaggtacgat agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct
     4801 gcatcgagat tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg
     4861 ctgcctcttt gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg
     4921 gttcatcagg aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc
     4981 cgataacttg gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct
     5041 tatccacttt ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt
     5101 ctgctaacgg gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa
     5161 tctgttttgc ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata
     5221 tatctgctgg gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt
     5281 catctgataa agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc
     5341 tcactgacga tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt
     5401 tggtatccgt ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc
     5461 aaaaaaagaa ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga
     5521 tagccaagca agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct
     5581 tggagattcc acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt
     5641 agatggccat tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg
     5701 ttttctgcct ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga
     5761 gtcgttaatt gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca
     5821 caaatcattc cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc
     5881 tcgctgattg tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca
     5941 cgatttggta gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc
     6001 gatacaggga tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc
     6061 agatccatga tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag
     6121 cgtttgacga aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca
     6181 caaatatacc agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac
     6241 aaatcataat agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt
     6301 agctgactga ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa
     6361 tcaagttcca tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc
     6421 tagaaaagat aagagcaaag ctcacctcat aacttggaaa taaataaaaa ttaaataaaa
     6481 tacatgtgtt ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt
     6541 ttctgctagg aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa
     6601 ctgtcttatt taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta
     6661 gctggaattt cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg
     6721 cgaacttcaa agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct
     6781 tgaatagttg aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta
     6841 gctgaagcaa ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc
     6901 acaagagata tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa
     6961 aaagaaatat tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata
     7021 gcaaggtttt taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac
     7081 tgtctttttc ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag
     7141 ggataattcg gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga
     7201 aacgttcata agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg
     7261 gtttgacagc gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg
     7321 ttggattgat tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa
     7381 gattctggtt catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag
     7441 tcactgcaga aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct
     7501 cttgttcgaa atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga
     7561 cgataataat tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc
     7621 gaatcccttc ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca
     7681 ttgcacgcat cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac
     7741 cagctgtatc tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg
     7801 cgtctcgggt cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat
     7861 tgatcaaaga ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt
     7921 cgtcctcttc ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa
     7981 tccctagtcc atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt
     8041 catagatatc gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt
     8101 tattacttct gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc
     8161 cgctgactac acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga
     8221 tctgatccat aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt
     8281 ctcgacctaa ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt
     8341 cgacaatcga gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg
     8401 ggcggccaac gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa
     8461 tctcacttag tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa
     8521 gtatttttat tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc
     8581 agctgctttc atctcagtca cagatacaaa atcaattatt tttcagaatc atcggttgct
     8641 ttttcttcta cttcttcttt caacgcatca cctaaaatgt ctcccatagt aaatccagta
     8701 ttttcttctg gtaattcata agactcaaca ttttttggtt cttcttctga agctggtttt
     8761 tcttctaaag ctttgatact caatgcaata cgatgttctt ctggatggat ctctaatact
     8821 ttcactttca cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt
     8881 gaaatttgag agatatggac tagcccttct actccaggga acacttcaac aaaggcaccg
     8941 aagcttgtca aacgtttaac tgttccgtct aatactgtgc caactggagc ttttgtatcg
     9001 atttctgacc aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca
     9061 ggattgatag acaacacttt gacatttaca tcatcattga cagccaatac atcacttggt
     9121 tttgctacat gactatgtga aatctctgag acatggacta gaccgtcaat tccgcctagg
     9181 tcgacaaatg cgccaaaatc agtaagacgt gcaacacgtc cactgagaac atctccctca
     9241 tggatagttg aaagaatttc ttttttcttt tcttctcttt cgttttccac taccgcttta
     9301 tgcgataaga tcaaacgatt ttctgatggt tcgatttcga tgattttaaa gctcaatgtt
     9361 ttgcctttat agtctgaaaa gtctgcaaca aaatgatctt ccaccattga tgcagggaca
     9421 aaaccacgta caccgacgtc aacgactaaa ccgcctttga caacatttgt aacaggtgct
     9481 tcgatgacct tgccctcttg gaaatctctt tcgatatctt cccaaacttt tttagcatct
     9541 aaacggcgtt ttgaaagtag gtagctgccg ttttctttat ctttgccgat cgtactgata
     9601 acgactaaat ctaatacatc accgactttt actacttcgc tgatatcttc taccggcaaa
     9661 gttgataatt cttttgccgg aacgactcct tcaactccag ccccttcgat tccgacaatc
     9721 acttgtttgt cttcaatgac taaaacttcg cccttaacaa catcgccaac ttttacttct
     9781 tgtacgcttt tcattgcgtc ttccattgac tggttgttct cttgattttg atcaaattct
     9841 gtcataacac ttgtcctcct aaccaacatt ctgccgtaag tacggtactg tttttagttt
     9901 aattgattat tcaaaaaaaa taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt
     9961 tttcattttt tttcttaatt taaaaaagaa gataaccttt tttcaaaaca acagctttta
    10021 ttgctgcaac gacttctgga atcgatttgc cagtcgtatc gatacgaaca gcatcctctg
    10081 cttgcttcag cggagagacg tcccttgttg aatcataatg gtctctttcc tgtatctctt
    10141 tcgtcagtgt ttcaagatca gtcggaattc ctttttcttg gttctctttg aaacgtcttt
    10201 ctgcacgctc acttacgctt gctactaaaa agattttcac ttctgcattc ggtaatacag
    10261 ctgtcccgat gtctcttccg tccatgacaa cgccaccgcg ttcccctatt tttcgttgga
    10321 cagcaactaa ttcttctctg acttttccat gtgcagagac ttcagaaaca gcttttgtga
    10381 catctggttg acggatttct aaagtcacat cttttgatgc tatgaataca tgctgtccat
    10441 cttcttgttg cgaaaaagag ataggatact ttttgatcag cgaaactagc ccttcttcat
    10501 ctgaaaaaga tacattgttc gtaatagcta aataagttac tgcgcgatac atggcaccgg
    10561 tatctgtata aatataattc aattcttttg ctaatatctt ggcaacggta cttttcccag
    10621 atgaagcagg cccgtcgatt gcaatactga tttgtgtcat gatataactc ctttttctca
    10681 gaaaaaaagc cgcaaagcgc ggctattatt atttaattct taattcttga ccagggtaaa
    10741 gcataaagtt gtttggatct aagccattca attcaaacaa ttgatcagtg gtgatccctg
    10801 cacgctcagc cacttgtttt ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg
    10861 cagctgatga agaaggtgtt gtttctgctg cattgctgct tgaatcccct gcttgataag
    10921 ttgcctcact tgaggaagtc ggtgttgtct ctgctgcaat actgctttct gacgttacac
    10981 ctgtatcttg agacacagta gattcttccg cgattgtact agattctact gttgaagtct
    11041 cagttgactc ctcttttgtt gtacttgtag aactttctac tactgatgtc gaagtttcag
    11101 aagaagttgc tgctgttgca ttgttgcttg atccatttct gatccagaaa taagcacctg
    11161 ctggaatagc gatacataag accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc
    11221 ctttcttttg ttgtctctga gaacttcttg atgaaacttc atcatctgta tcgtagattg
    11281 gttgttccca tggttcttgt gtttcgttat tttctgtaga ttgatgtcta tcttttctgc
    11341 tcactgtttt acctcctaaa ctctatcgtg tgtattatac cataggaaaa agtgtcttgt
    11401 cctaaaaaat caatgttcca tgtttttttt aaataaattt agtaaaatcg tttcccaatg
    11461 aagttgttca gtcttagaaa aaagcttcct ctttggcaga agttctgctt ttgctccatc
    11521 aatatcacaa caattctgcg gcttttcgga gagtttttcg ccaaaataag ctaagatgaa
    11581 ttttctgcgg cagttttctt cattaatata gcgaagcatc tgctgcaggc ggaattcttt
    11641 ttctttttcc tgacgtttga gccgttccac ccaattttct ggtttttcag actgctggat
    11701 cagctccatc catttttttt gcagctcatc aaagggagct tgctttgtgg catattccaa
    11761 ataaagctca aaactttgtc gctgttctct agaaagttga ttaaagaaat aatggatacg
    11821 ttcatcccca ctttgataaa aaagaattgc tgcactttct tcctgatctc tgcctgctcg
    11881 accgatttct tgtacatagt tttctagact gtctggcaaa tcataatgga ccacataacg
    11941 gatatcagat ttatcgatcc ccataccaaa tgcattcgta gcaatgagaa actgcagctg
    12001 attttttaca aactgctgtt gaagttgtct tctttgagct gcatcaagac caccgtgata
    12061 gtatccaact gtaaaacgtt ccctgaataa atgatacaat cgttcgactt ctttttttgt
    12121 tgcacaataa atgattgccg ctccttgtgc tctttccatg aagtgttcaa gttcttgttc
    12181 tttttgctgc gttttccgaa caaaaagcga gatattttgc cgattgactg attgtctgat
    12241 ctcttttggt ggtcgatcaa atagtacatg acgtatatct cttgctacta aatctgttgc
    12301 tgtcgccgtt agtgccagtg tgaccgggtt tcctaaaagc tttcggattt ttcctaactg
    12361 ttgatattct ggacggaaat caactcccca ttgagacacg caatgagcct catctactac
    12421 atataacgca atattttgtt ttttcagatg ctctaaaaga gaaggattag tcaacatttc
    12481 gggactcaaa aacaaaaatt tatattgcga tagatgacgg aggacgtatc ttctttcaga
    12541 ttgcgaaagt aaactattga aagcagctac tcgtttctcc cctcgttttt gtaattgcgt
    12601 cacttgatct tccattagag aaagcagcgg tgacacgatg atgaccagac cttccattaa
    12661 ataccccgtc aattgatagc aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt
    12721 atcttctcca tcaagcaacg cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa
    12781 tccaaaatat ttttttaatt catttattaa cattctgtat cgcttccttt ctcaatagat
    12841 agatttgata caaacgaaat tctccataat ctaattgtcc atctacattc cattcgctaa
    12901 agcgccactc tcgcacggaa tccttatttg gttccaaacg ttcgattgtt tctgcagaga
    12961 gaatccactc gtatggaaaa tcatcaaaaa acaaggccca ctcaataaga tgatctgtta
    13021 ctgttcctct ttttaaatga cggattgcca tgatctggtc aattgtctga ccattcataa
    13081 acatctgctt ggtgatcatc atgctttgat tgaaattttg ctgtaataat gaatcgatca
    13141 aagcgtgcca aagcgaatct ggccgttttt cgatttgtgc taacaaaagg tggatacatc
    13201 tgctttgttg cagataaacc tctgactcat cattgttttt tgccagctga taaggaagca
    13261 gccctgttcg ctcatttcca gataattggt tagctaaaaa atctgctgct tcttctggta
    13321 tctccgaaaa cagctgcacc aaactctcat acgcagtatc aatcaaaatt tcacgtggca
    13381 attggctttc actaagccat ttttttagtt ggaaagtata aaaaggactt gtctctgctg
    13441 gaagatagtc ttgaataccc gttgccagat tagaaattac ttgaacagca aatttaatga
    13501 gccgccaact agtttcccct gttctgccat agcggtcaaa acgcaaacca gtatgttcta
    13561 tttgatgttc ggacaacata tcagcccctg caggcgttag ctttgcttca ttttcattta
    13621 tttcgatcca accttgctgc gcaatttttt gcaaaatttg ataaaactta tcctgcttca
    13681 attctggaaa actgttatgg ataaacaaaa gttcgtgggt aaatccataa ataagtactg
    13741 aagtcgttcg ttttcccaca aggagttggt aaagagaact aatccgcagc ttgtgtcctg
    13801 atgaaaatag cgataaaata aaaacaatca tattattctc cttaagttag gatggttaga
    13861 aatgtcacta ctatgtaaac ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat
    13921 cgaagcgcca gatattttcg attatgatga agaaggaatc gttttatttg cccaagaacc
    13981 agaggctcat caacagttcg tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca
    14041 aaaatgtcca gttcgagcga ttttattaga aaataaaata taaatataaa cgaaacttta
    14101 aaaacgtaca ctaagttctc taaacttgaa aataagccgg gatctgtttc tcttgttccg
    14161 tgaaatcccg acttattttg aaagattcat ttgctatcta tagttaattg actagttttt
    14221 tcacggaccc tataggaccc atttatatta tttcaacttt ttcagccagt tttccaatgt
    14281 attgacgatc acagtttgct ggtctgcgtt agaaatttct gcctctccat ctcctttttg
    14341 ctcaccatag ctgccaaaac ctccatgatt tccgccattg attgatacat actctgttct
    14401 ttccggtaaa tactgcttag cttcttgata agcttcctga ttcaatactt tatcttttgt
    14461 cgccgtgatc gataaaacag gaacagctga ttctgtcaat gttccttttt tgtcgggata
    14521 acttgccatg aagaataccc cttttaaatg tttgtctgaa tgattcttat tgacataacg
    14581 acttgccatt acgccaccta atgaatggcc accgatcaca taatcggaat ttggaaaatc
    14641 ttctttgacc ttatccgctt tgtttccgcc catcactgct agatcaagtg gcattttgac
    14701 aatagcgact gaaaagccgt tttttgctaa atcttgcgcc caaatactat aactttctgg
    14761 ctctacgaga gcaccaggat aaaaaataag taatggagac tttccatgat tctttttctc
    14821 tccttcaaaa aataagtaat cttctttgtt ttttccttct tttaccaaca cttgcgcttc
    14881 tgacgatggt tcgtacgtat tttgctgtat atagtaataa ccggctccac cgagtaatcc
    14941 taaaatgatg actaagccaa ttagtattcg tctaaatatt ttcactttct cccacctttt
    15001 ctatctgagt gacccctttt tcttaatcaa gtatgattat accttaaaaa aataagagag
    15061 aaatgcaaac tttccagcat caaataaaaa aagacggaat atccatttga atatccgccc
    15121 tttttgtatg tttattttta tttagcacat aaacgataca cagcttctgc ataaatttcc
    15181 attgctttat acatactact taattcccat cgttcattcg cctggtgcat aaagtcagga
    15241 gtatcaggga acatcgcacc gaatgccaca cactggttca tcgtacgagc aaaagtagcg
    15301 ccgcctgata caaacggttc agtcatgtct ccagtaatat cgcgatatgt ccccaataga
    15361 tttttcacta actcactgtc tagtggcacg tataatgaat caaggaagtc aaattcttca
    15421 taagtcaacc catatttttc tgctgtttta gttaatttag ctactaaatc atctttcttg
    15481 aatgtaacag ggattctcat atctacaccg atttttgttt gttctggtgt gatttccaaa
    15541 cttgcaaagt tcatcgttag ctcgccggat tgttcatctt ctgttttgcc cacgacagct
    15601 tcaccagttg cattttcttg aactaatttt cctaagaaat tgattgggcc aaaatcaaat
    15661 acttctgaaa gagcaattgc cagacgagaa atagcattca ctccttgtgc tgcatctttc
    15721 gcgtggatac ttttaccaag aacgacgatg ccttcacctt gttcttcgaa atcgaattcg
    15781 tgttttttca atgcttcttt tacttcagaa agcttttctc cagagtaagg agctgcatca
    15841 ggaacaacat tcaacgctcc tccagctttt acagaaaact cgttcgttcc tggtcctgtc
    15901 aagtaagctt gcaataagcc tttttctgca taaattaaag gaaattcagc atctggcgca
    15961 aatccttgcg taattccttc ttctttttcg ttatattttt ctaaacaacg ccataaattt
    16021 tcttcatctg taccaaagat aaaacgaata cgtgtcttga attccacgcc agcatcaatc
    16081 aaagctttga cggcatacat tgccgcaata gaaggacctt tgtcatcttg ggatccgcga
    16141 ccaactaacc agccgtcttt tatagttgga tcgaatggtt ttgtttccca attattttcg
    16201 tcccctgctg gaacaacgtc catatgacac aagataccga ataattcatc tccagagcca
    16261 atctctgaat aaccgtaata gccttctgga tctttaaatg ttctaaatcc tagcttttca
    16321 ctgatttcca atactttatc caaagcttca atcacttttt taccgaaggg atgtccttgt
    16381 cctgaatctg cttcatctag tacggaagga attcggatca attcgctcaa tgattctaat
    16441 gcttgttcat gatgttgttc tgtcacaaat tttttcatga tattatcctc ctaatatgtt
    16501 aaaaagggtc atgtcaaatt cctccgacac aacctcttct tctcaaataa tgacgtttta
    16561 agcctgctcc atcagcaagc agaatgttct taacaaatga tgaaagaaaa aacactcact
    16621 gaatggagct attgacttgt ccacgtgctt atcgatagcc gtttatcggt ttcttcattc
    16681 ttatgataga ccttgtacct gtatttgtct aggtttagaa cattgctgct acacctagga
    16741 tcacacagat ggctacaaat aatatcgcaa ttaatttacc ggtaaatttc caccacactg
    16801 aaacatcgaa acgagcaatt gctacagcac ccattacgat agcagatgtt ggtgtaatta
    16861 agtttactaa accagatgca gattgataag cagtgattac taaatcctta ccgacgccgg
    16921 caaattcacc cataggtccc atgattccca ttgttgcagc agctaagccg gaagttgaag
    16981 gaatcaagaa tgacataggg atatagaaaa taaatgtaat aatgatgaat acacttgaag
    17041 ataatgaact taatccttgt tcgccccaat gtaagatggt gtctgtgatt aatccattgt
    17101 tcataaccac ttggatacca cgagcaacag ctactacgat tgctacaccg atcaaatcag
    17161 aagcaccagc taaaaattca gagacaaatt cagactcttt catgccataa gtgattgcta
    17221 ctacgatgga cattactaag aataacatag taatttctgg gaagtaccag tcacctaatg
    17281 gaagcatgct tttacctaga accgtaccta gtaccggaag gtttgttaac cagtttgtca
    17341 atgattcaaa aatcgtgaaa ttcgcattca aggttgtcca aggaatcaaa ctgatgatca
    17401 tgataccaaa agtaataaag aacaatacat taacatgttt ttgttttttc gtgattactt
    17461 cttgacgacc gattgcttca atatcgaaat gcttcatatc ttctttacgt tggttgtata
    17521 ctaatgattt tgttggatct ttttcgatct ttgatgcata acgatacaca tatacaatag
    17581 aaatagctaa caaaataacg aacatcaaga atcgaagacc aataccttca cccatcgaaa
    17641 tcccaacagc ttgtgaagct acgcctgttg caaaaggatt taccgttgaa gctagacagc
    17701 ctacttgaga ccctactaat acgattgcta cagctactac tgtatcaaag cctacagcca
    17761 tcatgactgg gatcagtaaa gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag
    17821 agccgcctaa tgcaaataac agcatcaata ctggaataag cattttttct tttcctttga
    17881 atttcttcac aactgaagca atcccggcat ctagtgcccc tgttttgttg ataataccta
    17941 aaaatccacc aacaaccaaa atgaagaaag agattgggat tgcagcaggt gtttctttag
    18001 ttagctcccc cgctggtaca cctagcatcc cattgattgg cgccatgaaa atatcccaca
    18061 gcccttgagg attttgttgt actcgttcat atgatccagc gacgatgttg cctgcatcgt
    18121 ctacactata gtgcccagct ggtataaacc aagtaagtac agcgattact gcaataataa
    18181 taaataagac cgtatacgct gaaggcattt gaaatttttt cttttttgtt tgttccatct
    18241 tcttcttcct ttcttttccg ttagagacaa caattttggc tctaatgtta ttttatcaaa
    18301 aagagtatca gaacaaaaat aataacacgc attttgtaaa actttt
//
LOCUS       DAZULY010000031        17973 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000031 DAZULY010000000
VERSION     DAZULY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.031"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(247..1974)
                     /locus_tag="ACG81A_000716"
     CDS             complement(247..1974)
                     /locus_tag="ACG81A_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFS5014107.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1967..3160)
                     /locus_tag="ACG81A_000717"
     CDS             complement(1967..3160)
                     /locus_tag="ACG81A_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS5014108.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3347..3991)
                     /locus_tag="ACG81A_000718"
     CDS             complement(3347..3991)
                     /locus_tag="ACG81A_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5014109.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4085..4219)
                     /locus_tag="ACG81A_000719"
     CDS             complement(4085..4219)
                     /locus_tag="ACG81A_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFS5014110.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4221..6353)
                     /gene="feoB"
                     /locus_tag="ACG81A_000720"
     CDS             complement(4221..6353)
                     /gene="feoB"
                     /locus_tag="ACG81A_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFS5014111.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6350..6823)
                     /locus_tag="ACG81A_000721"
     CDS             complement(6350..6823)
                     /locus_tag="ACG81A_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFS5014112.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7224..7448
                     /gene="nrdH"
                     /locus_tag="ACG81A_000722"
     CDS             7224..7448
                     /gene="nrdH"
                     /locus_tag="ACG81A_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFS5014113.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7607..9766
                     /gene="nrdE"
                     /locus_tag="ACG81A_000723"
     CDS             7607..9766
                     /gene="nrdE"
                     /locus_tag="ACG81A_000723"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFS5014114.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9816..10781
                     /gene="nrdF"
                     /locus_tag="ACG81A_000724"
     CDS             9816..10781
                     /gene="nrdF"
                     /locus_tag="ACG81A_000724"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFS5014115.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10870..12009)
                     /locus_tag="ACG81A_000725"
     CDS             complement(10870..12009)
                     /locus_tag="ACG81A_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5014116.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12318..13031)
                     /locus_tag="ACG81A_000726"
     CDS             complement(12318..13031)
                     /locus_tag="ACG81A_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFS5014117.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13285..13986)
                     /gene="nagB"
                     /locus_tag="ACG81A_000727"
     CDS             complement(13285..13986)
                     /gene="nagB"
                     /locus_tag="ACG81A_000727"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFS5014118.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14220..15752
                     /locus_tag="ACG81A_000728"
     CDS             14220..15752
                     /locus_tag="ACG81A_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5014119.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15943..17145)
                     /locus_tag="ACG81A_000729"
     CDS             complement(15943..17145)
                     /locus_tag="ACG81A_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5014120.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17227..17916)
                     /locus_tag="ACG81A_000730"
     CDS             complement(17227..17916)
                     /locus_tag="ACG81A_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS5014121.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
BASE COUNT         5610 a         3749 c         3150 g         5464 t
ORIGIN      
        1 aatatttttt gagaatgcac ttcgtacata ataaacttta atcacattag tctttcatac
       61 ctacgatcga tctctttgac tcgcacccat aaattcataa tagtttgtcg gcgtctttgg
      121 acgtttcgaa gatgagctac gcattctctt tttttataga caaaaaagat agagacgaac
      181 atggagcttc caaatttgga tatttgatcc aattttggaa gctccattta tttcgtgtct
      241 gtctttttat tttttccagc tttcgactaa ttcttggttc tctttgatcc agtcttttgc
      301 tgcttgctgt ggatcttttc cattgttgat ttccaacata actttttcca tatctttttc
      361 tgaccaatgg aaattgtcta atactttgta agcttctggc tggtcttctt tcaatccttt
      421 tcgaaccatc gtatggatct gctcttcgct tcccattccg ttttcaggat cttctaagta
      481 tttcagatca tatttggcaa acatccaatg aggtgtccat cctgtaacta caattggttc
      541 gtgttttttg atcgcttgac ctagtgcaac ggtcattgct cctgaagaag atgtctctac
      601 tttccagtct ttcaagttgt catatttttg gatcgtgttt tcagcagccg taactacacc
      661 agctcctggc tcgattccta tgattttctt acctgcctga tcggttaact catcgatcga
      721 atttacatcc atgtaggctg gcactgctaa accaactttt gctcctgtca aattggcacc
      781 taggtcttcg acttgatctt tatattgtgc atattgtgaa ccatgagttt taggtagcca
      841 agctgaaacc atggcatctg actcgccatt tgaaacagat ttccacatga ttgaattatc
      901 taatggcgtc atcgttacat catagcccat ttgtttcaat acttcaccga ctacatttgt
      961 tgaagcaact tctgtatccc attcaacata agataagttg atacgttttg tttctttggc
     1021 agatgaaaaa ctccctatac caatcgcacc taagatcact actactacag caccaatgat
     1081 cagtccttga cgttttttac ttttctgtgt tgttgctcct gcagcttttt tattgtttgg
     1141 ctggttcaaa tgctgtgtaa aacggtcaat gatgattgct aagataacta atgcaacccc
     1201 gttgacaaat ccattcccga cttgtgctct ttgcaatgct gataatacgc cgcgtcctaa
     1261 gccgggtgca ccgatcatag aggcaataac gaccattgaa agtgacagca ttgttgtttg
     1321 attgatccct gccatgatcg tacttttagc taaaggcaat tctagtttga atagtttttg
     1381 acgacttgtg ctcccgaatg aatcagaagc ttctactaat tctttaggaa cttgtcgaat
     1441 ccctaaattc gtaaaacgaa cggttggtgg cagagcaaag atgactgagg caaatacccc
     1501 tggaaccatc ccgataccga aaaatgcaac tgctggaatc aaataaacga atcctggcat
     1561 tgtctgcata aagtcaagga tcggtttgat aatactttgg gctttactgc tttttgccat
     1621 caagatccct aatggtaccc caatgatgat cgagatcaca ctagataaaa gaacaagtgt
     1681 gacagtattc atcaaatcat tccatagacc ttggttataa ataaaccata gaccgattaa
     1741 tgtgaacaac gtaagtcctg gacgtttctt agaaataaag aaagctgcta ccgtcaataa
     1801 caagataaac aaaggagctg gaatcactaa cagtaaattt gtaatccccg acatcactgc
     1861 ctgtccaatc gtttgtaaaa atgaaaatag gccagaaaaa gttgtcgtaa accattctgt
     1921 gatattttct acccagtttg ccactggtaa ttgatagttc aatagattat tcatgttcac
     1981 tcacctcgct tgtttctgct aatgcttcaa tcacactacc tcggatgacg acacctttta
     2041 attttccatc ctctacaact gcaagtggag tcggtgagtc ataaataata ttgaaaatat
     2101 cacttaccac catgtctttc ccaatcaccg tcacattttc atctaggaag tcgatcaatg
     2161 gacgtttttg cttcctagct tctaacgctt gatctgcagt gatgatccct ttcaggtgtc
     2221 tctttctgtc aacagccatc agcatgctga cttcttcatt acgcatcctt gtcaatgcaa
     2281 cgtttggtcc atcagattcg atattcgtcg tcaatgcagg taccatgatg ttttgagccg
     2341 taagcacttt ggaacgatcg acttcttcca caaattcacg tacataatca tttgctggat
     2401 tggtcaatat ttcttcacct gttccgatct gcatgatttc gccgtctttc atcaaggcga
     2461 ttctgtcccc aatccgtaaa gcttcattca aatcatgggt aatgaaaata atcgtttttt
     2521 gaacattggc ttgcaggtcc agtaattcgt cttgcatttc tcgccggatc agcggatcaa
     2581 gagccgagaa cgcttcgtcc atcagcaaga tctcaggatc atttgctaaa gctctggcta
     2641 ggccaacccg ttgttgcatc ccaccagata actgactagg atattggtct ttaaatgaaa
     2701 gcaaactaga attttctaat gctttttctg ctttttcttg tcgttcttct ttaggtacac
     2761 cacggacttc taacccgtat tctgtatttt ccaagatcgt tcgatgtgga aacagaccga
     2821 agttttggaa aaccatattg attttatgac gacggacttc tcgcaattct tctttgctta
     2881 acttcgatac atcttctcca tcaatataga tactgcccga agttggctca atcagacgat
     2941 tcaatagacg gatcaatgtg gatttcccac tcccggaaag ccccatgatc acgaagattt
     3001 ctccttcttt tacgtcaaaa ttcacgtcat acacaccaac cgttgcaccg gttttcttta
     3061 aaatctccgt tttcggctgg tgatcattca tcatatctaa agcggcttgt gtctttttac
     3121 caaatatctt tgttaaatgt tttacttcta cttttaccaa acaaattctc cttttttatg
     3181 taaattcatt tttctttctg aaaaaagtga ccacactatg ttaacatcat gtagtcactt
     3241 tgtcaaaaat gaaagaaatt tgcttgtcca aactgaaaaa aacttggcta aatctttgta
     3301 aataaaaggt ttagccaagt taatattttt ttaatatttc acacaatcat tctgcaagct
     3361 gatgctccgg gtaaaaaaag tttgtcatac gctgtagtgt atattcattt ccaataaaat
     3421 gaacgatgtc ccctgccgta aatttagcat aggggccagg tgaaacaagc agttcttcat
     3481 tatgagaaat tgcgataact gtggcgccag tcgcctgcca gatattcaac tcgctaattg
     3541 aacggtccag atgcattgct ttttctgata aagataattc aaacggaagt aaaggattca
     3601 tcttatgtat tcgcttcgtt tgattgacca gctgatccaa taattgagaa aaatgattca
     3661 gctcatcttg ttgcttctcc acacttgcca tgatttctgc ttttgtatct tggatggatt
     3721 gtacatcttg atattgcaat aggaaatctt ttgctttttc cttcgaagca acaaatgccc
     3781 cacttccatg ccgtacttcc atgattccaa gatctaccag cacattgata gccttgcggg
     3841 tagtctcagg agaagcattg tacgtactgg caagtgttga gcgggcatgg atcttctcac
     3901 ccactttata tcggttttca acgatacgtt cggctaaatc aacagcaatc tgctggtagc
     3961 gcgggcggac gatttttttc tttccagaca ttttctttcc tctttctttt tttactttcc
     4021 ctcatcttac tatgctctag caaaataagc aaaaaagggg agaatagcct cttgcaacga
     4081 ctgatcatcg cttgatctgt tcctttttta ctgggcaagc tgtttgacag tcatcacaat
     4141 tcttaccgga ttttaaacga gtatagacaa ttgcgcctgc acttccaaag attaacacac
     4201 ttaataagaa tgtcgccatc ctattcctct ccttctaatg acaatatagt ataaaactgg
     4261 ttcttggatt ttggctgacg gaacaaaaca taaacgagtc ctatcatcag taaaatagca
     4321 agatacgtcc ctaacgctac ctgtccaccc tccaataagt gtcccaactg ataaatcaca
     4381 aaactaacta aataagctaa accgcattga tagcctatgg cgatccaagt ccatttcttg
     4441 gcattcattt cccgatggat agccccaatt gccgcaaaac aaggcgcaca aagtaaattg
     4501 aaggctaaca atgaataagc tgctgcggga gtatatgtgt gttgtagcac ttgccagatt
     4561 tctctgccat tttcggatac ttccgcatga ccaaacaaaa taccaaatgt gccaataata
     4621 ttttctttag cgaccaatcc tgtaatcgct gcgactgttc cttgccagtt tccccaacca
     4681 agcggctgaa aaacaactgc caggattcgc ccgaaaaagg ctaagatact ttggtcttca
     4741 gaaacaggtt gagcataaaa attataagaa gagctgaacc agatcacgat actagaaaca
     4801 aagataatcg ttcctgcttt tttaacaaaa gacttgctgc ggtcataggt ttgtcttagg
     4861 accgctgacc attgaggaaa atgatatgcg ggcaattcca tgataaaagg agctgtatct
     4921 ccagcaaaca aacgtgtttt tttcaagctg attccagaga aaataattgc agtcatacca
     4981 agaaaatagg cagaaggagc tacccaagat tgatgaggaa aaaaggcccc tgctactaag
     5041 gcaataatcg gcaactttgc agaacaaggc atgaaggttg tgaccatgat cgtcaaacga
     5101 cgatcttgct cgttttcaat cgtgcgactg gccatgacgc ctggcactcc acatcctgta
     5161 gcaatcagca taggaatgaa ggattttcct gaaagaccga atttcctgaa aagtcgatcc
     5221 atgacaaaag cgatcctcgc catatatccg cagtcttcca gaaagctcaa gcataaaaac
     5281 aatataatca attgcggtag aaatcctaac actgctccca cgcctgcgat gatgccgttc
     5341 aagatcagct cctgcatcca tcccgctact tgccattcag ctaacaatct gccaacatgt
     5401 tctggtaacc atgtaccaaa caatgtgtca ttgatccaat ctgtccccat tgttcctact
     5461 gtttgaatag ccagataata gatcagccac atcacaaatg cgaagatcgg caaggctaac
     5521 caacgattcg ttgcaatctg atcgattttg tcactcatcg ataattggaa agaatcattt
     5581 gacacaacac agagtgtaca tagttgggta ataaattcat aacgttcatt gaccagaatc
     5641 gtctcactat cgtcgtccaa tagtttctct gttaactgga tcagttgctc gatctctttt
     5701 tgctgagaaa ctgctatctc gtaatcctct cttaccttct gatctcgttc aaaatatttc
     5761 attgcatacc agcgtttctt gccgctttgt tctcctagta catcttcaat ttcatgtaaa
     5821 gctgcttcta aacgtggatc atatgtacag ggaacaggaa tagaaggccg cttttttgca
     5881 gactcctgaa tcttttcgat tagtttttcg attccttgct ttttcaatac gctgattggt
     5941 aaaactggta caccaagacc ataggaaagc ttttctagat tgattttttt tccttctttt
     6001 ttgatgacat ccatcatatt cagaccaatg atcataggaa tgtctaaatc caataactgc
     6061 gtagttaaat aaagatttcg ttctaaattg gtagcatcaa taatgttgat gatcagttca
     6121 ggctgttcct gaaccaaata atctcttgca atcacttctt ctggtgtata aggagacaaa
     6181 gaatagatgc cgggtaggtc ctgaatatga atgttcggat tttttttcaa cgatccttct
     6241 tttttttcta ctgttacgcc cggccaattc cctacatatt gattcgaacc agtcaagaga
     6301 ttgaacaagc tagttttccc gctgtttgga tttccgacta acgcgatatt cattcttttt
     6361 cctccctttg tatcaagatt agttcggcct ctgactttcg taacgtcagt tcataccctc
     6421 gcactgtcag ttcgattgga tctcccagag gggctagttt tcggataact atttttacat
     6481 ttcgcgtcaa tcccatatcc attaaccgtc gcttcactgc gccttgtccg tggattgcta
     6541 cgacgatccc agtctctcct gcttttaatt gatcaagaga agtccagctt tcagaggaag
     6601 cagtcttttt attgacaaag atttgaccta gtatttcccg acttactgcc actcgatgac
     6661 tttttaccag taaaatggca ttgttgcctg ataaattgac gacgacgatt ttacttccgg
     6721 caacgatccc gatattttgc aaatggcgtt ttgttttttc ttctgtttcg attcgttcga
     6781 cttgataaac gaaattctgc tttgcctgcg ctaaggtctg catattgatt cctctcttgt
     6841 gaaataaatc atgtattaaa atcattcatt atatgttcta attttcttac gtgttacgca
     6901 aataaatgag aatcattctc acatagaata ctctcggtaa ttgagaatgt caattatact
     6961 tgtgatatta tgagcgaatc tttagtattg atagataaaa atgagaaaaa aatatttctt
     7021 catagttttc cacaattttt tctctattaa atttgctatt ttcccacacg atacttgttt
     7081 tcagctcttt tttcaaaaac acaaaactta gtattccaca gtttccacaa cacaatatgt
     7141 agtgtttgtt gtttgacttt tttactaaga gttttatact atctattata gataataaga
     7201 atgtgttaga ggaggcactc accatgaacg taaaaatctt ttctaaaaat aattgtatgc
     7261 aatgtaagat ggtcaaacgg tttttaacag aaaatcaaat tgcttttgaa gaagtcaata
     7321 tcgatgaaca gcctgaagca gtagactggt tgaaagcaca aggattccaa agtgttcctg
     7381 tcattacttc tgatgcagaa acagttatcg gattccgtcc tgatcagttg cgtaaacttg
     7441 ccagctaaca tgactaaaag aatcaaaacg tgatacatgg atttgaaggg cttgagacag
     7501 caccagtttt tgtcgcaaac ttccaaatcc ttttttaaag cttaaaaaca atgcaatgat
     7561 ttgcaatcaa gcatcatcaa atacgaaatg aaggttgggc gtacgtatga gtcttaaaaa
     7621 tttacaagat gtcagttatt tcaagctgaa taatgaaatc aatcggccag tcaatggtca
     7681 aatccctttg aataaggata aagaagcttt agttgccttt tttgaggaaa atgtcaaacc
     7741 aaatacgatg acttttctat ctattatgga aaaaatcaat tacttgatcg atcaagaata
     7801 tatagaagca gaattcattc aattgtattc accatcattt attgaaaagc tatatcaatt
     7861 tttggacgag caacattttt ctttcaaatc ttttatggct gcttacaaat tttattctca
     7921 atatgcgttg aaaaccaacg acggttccgc ttatctggaa acttatgaag atcgagtagc
     7981 cttcaatgcg ctttactttg cgaacgggga tgaagaatta gcacttgctc ttgcagacga
     8041 aatgatccat caacgatatc agccagctac accttctttc ttgaatgctg gacgtaaacg
     8101 tcggggcgaa ttggtgtctt gtttcttgac tcaagtcact gacgatatga actctatcgg
     8161 gcgctcaatc aactctgctt tgcaattgtc ccgtattggt ggcggcgtag ggattacttt
     8221 atcaaatctt cgtgaagccg gcgctcctat caaaggatat gagggtgctg caagtggtgt
     8281 cgttccagtc atgaaattat tcgaagatag tttcagctat tctaatcaat taggtcagcg
     8341 tcaaggagct ggcgtggttt atttgaacgt tttccatcca gacattattg ctttcctttc
     8401 agcaaaaaaa gaaaatgcag atgaaaagat tcgcgtaaaa actctttctc ttggcgtgat
     8461 cgtacctgat aaattttatg aattgacacg taaaaacgaa gatatgtatt tatttagtcc
     8521 atatagtgtc gaaaaagaat atggtgtccc attctcttat gtaaatatta cagctgagta
     8581 tgatcatcta gttgcaaatc caaacattcg caaacaaaaa atcaaagcgc gtgatcttga
     8641 aaatgaaatc tcaaaattac aacaagaatc tggctatcct tacgtaatca atattgatac
     8701 agcgaaccgc gagaatccaa tcgatggcaa gatcatcatg agtaatttat gttcagagat
     8761 tttacaagtt caaacgcctt ctgtgattaa cggcaaacag gaatatgaag tattaggaac
     8821 ggatatctca tgtaacttag gctcgacaaa tatcgtgaat ttgatgcaaa gccctaactt
     8881 tggtcaatct gtgcgtacga tgactcgtgc attgacattt attacagatg catctgatat
     8941 cgatgtcgta ccaccgatcc aaaacggaaa taagctgaat catacgattg gattaggtgc
     9001 catgggctta cacacttatc ttgcaaaaga acaaatggaa tatggctcag aggattcatt
     9061 agactttaca aatatttact tcatgctgtt gaattattgg acacttgttg aaagcaacaa
     9121 tatcgctcgt gaaagaaaac aagttttcca taattttgaa aaatcagctt atgctgacgg
     9181 cacttatttc gaaaaatacg taactggaga attccagcca aaatcagaaa aagtcaaagc
     9241 attgtttgaa ggcattttta tcccaactgc tgaagattgg aatgctttga aacaagcagt
     9301 aatgaaagat ggactgtatc atcaaaatcg cttggcagta gcaccaaatg gctcaatttc
     9361 atatatcaac gatacgagtg caagtattca tcctatcact cgcatgatcg aagaacgcca
     9421 agagaagaaa atcggtaaaa tttattaccc agcgccttat ctatccaatg agacgattcc
     9481 gtattacact tctgcttatg atatggacat gcgtaaagta atcgatgttt atgcaatggc
     9541 acaacaacac gtcgatcaag gcatgagttt gactttattc atgcgttctg aaatcccaga
     9601 aggcatttat gaatggaaga caacaacgaa gcaaacgaca cgtgatttga atattttaag
     9661 acattacgct ttccacaaag gaatcaaatc catttattac gtacgtacct ttactgatga
     9721 tgcagaagaa atcggaagca atcaatgtga aagctgcgtg atctaatatc atagatagat
     9781 tcgcttattc atattttaat taaaggagca ttctcatgtc agaaacatat tatgaagcaa
     9841 taaactggaa tgaaattgaa gatatcattg ataaatccac ttgggagaag ctgacagagc
     9901 aattttggct agatacacgt atcccacttt caaatgacct agatgattgg cgtacattgt
     9961 ctcaattgga aaaagataca gtaggacatg tatttggcgg tttgacactg ctagatactg
    10021 tccaatctga aagtggcatg gatcagttgc gtaaagatgt ccgcactccc catgaagaag
    10081 ccgttttgaa caatattcaa ttcatggaat ccgttcatgc caaaagttat tcttctattt
    10141 ttagtacact gaatacgaaa aaagaaatcg aagaaatttt tgactggaca aatacgaatc
    10201 cttatctgca aaaaaaagct gagcgtatca atgaaatcta caaacacggt acaccgctag
    10261 aaaagaaaat tgccagcgtc tttttagaaa cattcttgtt ctattcaggt ttttatactc
    10321 ctctttatta tttaggaaac aacaaattgg ctaacgtagc agagatcatc aaactaatca
    10381 tccgagatga atctgtacat ggaacctaca tcggttacaa attccaatta ggattcaacg
    10441 agttacctga agcagaacaa gaaacgttaa aagactggat gtacaatctt ctttatgagc
    10501 tttacgaaaa cgaagaacgt tatacagaag aattatacga tccaatcggc tggacagaag
    10561 aagtcaagac attcctacgc tataatgcca acaaagcatt gatgaaccta gggatggatc
    10621 cactctttcc agatactgcc aatgatgtca acccaatcgt aatgaacggg atttcaacag
    10681 gcacaagcaa ccatgacttc ttctcacaag tcggaaacgg ctatttgtta ggtcatgtag
    10741 aagcgatgaa agacgacgat tatttgattg gattggaata aggatttatt ttaattcaaa
    10801 aatttttcga aaaataaaaa gagtttagcg tacagatatt ttaaatgtac actgaactct
    10861 tttttatgct tattcacttg tttcttccag cacttttgga ctgtctaact tttccgaaac
    10921 atgttccatt cttctcaata aatagatttt ccggatattt aatattatcg tcttaatcac
    10981 tgcatagaca ggtatgcaaa tcagcatgcc taatattcct gcgacacttc ctgctccaat
    11041 caataccaag atgatcgtca aaggatggat attcattgat ttcccaaata acaacggctt
    11101 gatcaaattt ccatctaatt gttggatcac taaaatcgac aacgccatat acaaagcttg
    11161 aaatggagag ataaacaaac cgacaatgat tgctggtacc gctccaataa aagggccgac
    11221 atacgggata atattcgtga ttccgcaaat gaccgcaagc aacaagctat aaggctgatc
    11281 gaatattctc attgccaaaa agctcattac accaataatc aaagcatcta agatcgtact
    11341 gctgatatag gcagataatg tttcgtttaa ttcttttacc gtttggcgta actctttacg
    11401 aatcgctttc ggaaagaagt gagacaacgc atcaataaat ttgtgcccat ccttgaacat
    11461 aaatagcaaa ataaatggaa cggtaaataa caaaataaaa aattgcatca taaatgaaat
    11521 gatttttgag acacttgcag ataaactgac taatatgata ttgaacaaat tactaagaga
    11581 taaatttgct gatgctaatt gttcttccaa attgaaattt ttaaactctt cccgctgcgc
    11641 caattcattt aaccatttgc ttgtttcatc tgcatacatt ggcaagctct gggtcaattg
    11701 aattgtctgt tccattaact gaggcactac attcatgaca gttaaaacaa ctaaaatgat
    11761 tacgaccaaa aacgtcaata aaaaaccgaa cactctcggg atccgatgct tttcgagaaa
    11821 aacaacgatt ggatcgaaca tataatagat aaaaccagca acaagcaatg gtaaaaaaac
    11881 tgccgaaagc attttcaata ttggactaaa aatattcggc atttgtaaaa taaagtacac
    11941 gccgatgatc gtgagtacta gctccactgt ccagaaaaat aattttgatt gtttaaaacg
    12001 tgattgcata catctcctcc aaggtccttt gatacttcta ttctatcatt taacaaagag
    12061 ttacgcgctg agattttcat ttgcttaaaa aaaacaaaaa aaataaacat attttaatga
    12121 gtagacgagt acttaaacaa aaacacggtt tggagtagtg acaaaaatta tactgcagtc
    12181 cctttttcca aataaacggt gttcaaaagc ttgcatcttc gtaataagcc cagccaaacg
    12241 aaaaatataa ggaactattt ttatttggct gggcttatta cttgatgtag gacatctatt
    12301 ttacaacctc agatcattta ttgcgcaata ctgactaaat gcaactttgt gatatttttg
    12361 tgtacggaca actcctcgat cacatcggca taagtgagcc cttttggaag atcaatcgta
    12421 tagatatttt tgtacactcg gtagtcatca acgaatttta cgtcaaaatt cacatcttcg
    12481 attacgatat ttttctcttc aaaataatcg gtaagaaaat ctttggtttc ttttctatgg
    12541 ataaattgga tttctaactt tttagtcgta ggtacatgga tcacgcgttt cactactgtg
    12601 agtgcaatag caatcccgat aaaactgatg atcgcaatgg aatagtaacc catgccgatg
    12661 gcgatcccta atccagcaat tgcccataaa gaagcggcag tcgtaagtcc cgtcaccgat
    12721 tgtttcgtga cgatgatcgt tcccgcgcct aagaaaccga caccactcac cacttgagca
    12781 atcagccttg cttcatctga acgaatcgtt ccagcaagct gtggatactc taaagcatct
    12841 cttaatgcac cactagcgat ctcaacttgg atcaatgcaa tgatacatgc tcccatacaa
    12901 actaaaatat gcgtacgcat acctgctgga cgatttttat attgtcgttc aaaaccgatt
    12961 gctccgccaa atagcatagc gatccctaaa cgtaaaataa tctctggaat tgtcaatacc
    13021 atatcattca tctaaacacc ctccagtcca atatatcatg tctgaaagga gcagacaaaa
    13081 aaagtgcaat ttttacctct tacgaaaaaa ggactgtgac aaaatttttg tcatagtcct
    13141 ttttatgaat aaacggtgtt caatcagctg accctcggta ttactcaaca aacggcaaaa
    13201 tatggggagc tatttttcct ttgtcttctc taatactcag gtcaaaacgc tgatttcaca
    13261 acctctttag cctactattg aaaattacaa cttacttgct gcttcttcat cgatgatcac
    13321 aacaacattg tcgtgatttt gcaatacact tgctggcaaa tgattcgtta caggtccttc
    13381 aatcatccct ttgattgcat cagctttgct ttcgccaaat gccatcaaga cgatttcttt
    13441 acttttcata attgaaccga ttcccattga aacagctttt gttggtacat cttctgcttt
    13501 atcgaagaat cgtttgtttg cattgattgt tgattctgtt aagtcaacta ctgaagtcgt
    13561 tccgtcaaat gaagcaccag gttcattgaa accgatatgt ccattttgcc cgattcctaa
    13621 gatttggata tcgattggat gtgcatcgat gatttgatca tactttttgc atgctgcttc
    13681 cagatcttct gcttttccat ctggtacaaa cgtttctttg aatggttttt tatcaaataa
    13741 ttggacatcc atgaaatgac ggtagctttg ttcatctgaa ccgcctagtc caacgtactc
    13801 atctaagttt acagaagtca tttcactgaa atccaaatcg cttgaagtca tttctttata
    13861 taaagtaact ggtgtgcttc ctgttgccaa tcctaatact tttgcacctt tgttcatacc
    13921 ttctttgatg atttcaaatg ctttttttcc gccttcttca gcgtttttta cacgaatgat
    13981 ttccatagtg attgctcctt tatattttgg tatagcccaa ttatagcatt ctttttttct
    14041 ttttacaata attagtatag accaaattgc atttttttca ttttcttgac aataaatagg
    14101 aatccgtgtt aggtttcata agatggttca atcaaacaat ccatgttgca tgaaaaccaa
    14161 gcaagtgatt cactgtggac gctttatgtc tgcagttttt ttatgaaaaa ggagttatta
    14221 tgagtatact gacattaaac aacattaccc agcaatttgg cgacaaaatc ctctatgaag
    14281 gtgtcgatct gcaattaaat gctggcgaac atcttgggct gattggacaa aacggagccg
    14341 gtaaaagtac attgattaaa atcatcactg gtgagatctt accagatgac ggacagatcc
    14401 attggcaaaa aaatattcac aaaggttacc tcgatcaata tgtggaagtc gatgaaacat
    14461 taacaatcga agaatttcta aaaactgcgt acgcaaaaga atacgaaaag gaagaaaaaa
    14521 ttgcttttct ttaccaaaca tatagcgaga ccttcgatga ttccttgctg gaaaaagctg
    14581 ggaaactcca aacggaatta gaccaaggag tgttttatca aatcgacacc ttagttgctg
    14641 agatgagcag cgggttgggg atcgatgtct taggtttaga ttcactgttg aaaaatctaa
    14701 gtggtggaca acgttcaaag gtgattttag caaaactact tttagaaaac ccagatgttc
    14761 tgatcttaga tgaaccaacc aatcacttag atgatcagca tatccaatgg ctgacgcagt
    14821 ttcttcagga ttttgacggt gcgtatatcg tgatttccca tgaccaagaa tttcttgatc
    14881 agatcaccac ccatatcgca gacatcgagt ttggaaagat tactaagtat actggccatc
    14941 taaaacaagc attgaaacaa aaggaacaaa atcgcgaaag ctatttgcgc caatatcatg
    15001 ctcaacaaaa acaaatcgaa aagaccgaag cttatattcg aaaatacaaa gccggttccc
    15061 gatcaacgat ggcaaaaagc cgacaaaaac aattagataa aatcgaacgg ctgacgcctc
    15121 cagcttcagc aagtaaagct gtctttgatt ttccattctc gcctattgtc acgacattag
    15181 cagtggaaac aactgattta gtcatcggat atgatcgacc gctacttgca cccatcaatt
    15241 tgaccatccg ctttggtgaa aatattgcca ttcgaggctt caacgggatt ggaaaatcta
    15301 ctttattaaa aacactgatc ggtgagattg aaaaaataag tggcgatttc cacttcccag
    15361 ataacacaaa aatcaattac ttttcacaag atctgtactg ggaaaatcca ttagaaacac
    15421 cactacaata tttaagcagt caatttccaa aagcgactat caaagaatta cgtcgccaac
    15481 tagctaaagc agggttagtt aatcaattag caagtgaacc tctcactaca ttaagtggtg
    15541 gggaacaaac taaggtgaaa cttgcacagt tgacaatgaa ccaaggtaat ttactgattc
    15601 tcgatgaacc gacgaaccat atcgatcaag aaacgaaaga aagcttacaa gacgggatac
    15661 agaaatttcc aggaacagta atcatcgtct ctcatgaaca agaattttat caagatcttg
    15721 tgcaacgagt cattgagatt gaaccaaaat aaacgtttga ttgaatagtt gaaaaaaagg
    15781 agctgtgaca aaagttttgt cacaactcct tcttctgaat aaatggtggc caattagctg
    15841 accttcggta ttagctcaaa aaacggcaaa atatgaggag ctatttttat ttggctattc
    15901 ttattacttg atgcagaaca gcttttcaca acctcttttc gtctattttt tattttcgag
    15961 atttctcttt cctttaaaaa caactgtttg aaagaagaac gcacatcctt gaaaaacaag
    16021 cccgctaacc gttacaccta accagccata ctgctgccac attgtcgtcc ctagcaatga
    16081 accagccgca cctccaataa agtacataaa cataaataca gtattatttc ggttgcttgc
    16141 tgcttcacct agcccttgta ctcgtgtttg attggcgact tgaccaaatt gattcccgac
    16201 gtctaataaa ataatagcta aaagcaaaat ggccaaatgg cttccgccaa ccaataaagc
    16261 aataaagctt attgtttgca taacaagtcc catatacacg atttttcttt cagaataaga
    16321 atctgccagt cgtcccacga taggtgcagc aactgctcca gacaaaccaa aaatcgctaa
    16381 tatccctgct tcgaatgttc cccaattgta ggcgggactg ctgatataaa aaatcaacgt
    16441 agaccaaaag atcgaaaatg ttccaaacat gaaaaatcca ctgactgccg cttctcttaa
    16501 tagacgttga gaagcaacta aatgcggcaa acttttaagt gaatcaaggt atgaaacagc
    16561 tgtactactt tgctggacag aacgcggcat tttccagtaa agcagaagtg taagaattcc
    16621 gactgcacat aaagcaaata aatagatcat ccgccaagaa aaaagactgg caacaatacc
    16681 agataccgtc cgagaaagca aaatccccac gagtaaaccg ctaagcaatg tacccatgac
    16741 cttccctctt gcttttggtc ccgctagtac cgcaccgtaa ggaatgatga tttgcggcac
    16801 gatcgaaagt aatcctatta aaaaagaagc gcatgcaaat aacgtaaacg aaggcgcaaa
    16861 aaaagcggcc aacagagaga cggcagataa cgctgccatt cgaatgatca attttggacg
    16921 atccaccacg tcccctaaag gaactaaaaa cagtaaccct aacgcatagc ctaattgggt
    16981 caacatcgtc aatatcccga ctgcacttgt gctaacagat aaactagctg ctacttttgt
    17041 tccaatcggc tggatgtaat acatattggc aacaacaacg ccacaagtga ttgataatag
    17101 taaaataagc gaagttgtta aaggtttttt ttcattactc ttcattgatt tcccccattt
    17161 cctatcttag tttacaactt atggaaaaga ttggaaatag aaaaatacaa ctattcgcca
    17221 cacttattat ttgtgctttt gttttccttg ttttttcttg cgatctttat aaatgaagac
    17281 aactaaccct actagcacag taacacttgc aaccaacgtg actgcagttt tgttatatcc
    17341 gtgaaacaga gcttctccac cacacgcata caacacagac gaagggatga ctccaatcac
    17401 gatcgacaat atgatttgct gtagaggcaa tttcatcgca tctgcagcaa aattgaccat
    17461 agaagatgga ataaccggta tcatatagcc taacattaca cctatttttg gatgtttcat
    17521 ttgtcggatt gcttgcaccc aatgattcgt ttcggttttc ttatctaaaa attttaagcg
    17581 atgcataagg aaaattgcag tgatgttacc aagagcatta cccaaaatgt tgatgatcgc
    17641 acctgtcagt ggtccaaagc tcaaaccagc aatgactcca actactgaag taggaatacc
    17701 tggaacagca cacatcaccg ctgtcaatac gatcaagagt atcgatgcaa aagtcccatg
    17761 cgaacgaata tgtgcgagta gctctgcctt actcgctctc ggatttaaaa ataattggat
    17821 gtctgtctga tattctaaat acaaccgata gcccactacc agtatcagaa tgattcccaa
    17881 cgagatcaat atcaatcgga ttattttctt tttcatattt cctcctgaaa aaaatctata
    17941 ttcattaaat aatagtttaa cataaaaaca aaa
//
LOCUS       DAZULY010000032        17717 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000032 DAZULY010000000
VERSION     DAZULY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.032"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..1255)
                     /locus_tag="ACG81A_000731"
     CDS             complement(47..1255)
                     /locus_tag="ACG81A_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5014122.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(1274..1552)
                     /locus_tag="ACG81A_000732"
     CDS             complement(1274..1552)
                     /locus_tag="ACG81A_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014123.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1745..2401
                     /locus_tag="ACG81A_000733"
     CDS             1745..2401
                     /locus_tag="ACG81A_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5014124.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(2843..3640)
                     /locus_tag="ACG81A_000734"
     CDS             complement(2843..3640)
                     /locus_tag="ACG81A_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFS5014125.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(3637..4821)
                     /locus_tag="ACG81A_000735"
     CDS             complement(3637..4821)
                     /locus_tag="ACG81A_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFS5014126.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(4846..6093)
                     /locus_tag="ACG81A_000736"
     CDS             complement(4846..6093)
                     /locus_tag="ACG81A_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS5014127.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            6308..8053
                     /gene="dnaX"
                     /locus_tag="ACG81A_000737"
     CDS             6308..8053
                     /gene="dnaX"
                     /locus_tag="ACG81A_000737"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFS5014128.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            8073..8387
                     /locus_tag="ACG81A_000738"
     CDS             8073..8387
                     /locus_tag="ACG81A_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFS5014129.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            8412..9008
                     /gene="recR"
                     /locus_tag="ACG81A_000739"
     CDS             8412..9008
                     /gene="recR"
                     /locus_tag="ACG81A_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFS5014130.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            9072..9716
                     /gene="tmk"
                     /locus_tag="ACG81A_000740"
     CDS             9072..9716
                     /gene="tmk"
                     /locus_tag="ACG81A_000740"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS5014131.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            9731..10060
                     /locus_tag="ACG81A_000741"
     CDS             9731..10060
                     /locus_tag="ACG81A_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFS5014132.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            10074..11012
                     /gene="holB"
                     /locus_tag="ACG81A_000742"
     CDS             10074..11012
                     /gene="holB"
                     /locus_tag="ACG81A_000742"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFS5014133.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            11048..11872
                     /locus_tag="ACG81A_000743"
     CDS             11048..11872
                     /locus_tag="ACG81A_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFS5014134.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            11865..12212
                     /locus_tag="ACG81A_000744"
     CDS             11865..12212
                     /locus_tag="ACG81A_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFS5014135.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            12281..13153
                     /gene="rsmI"
                     /locus_tag="ACG81A_000745"
     CDS             12281..13153
                     /gene="rsmI"
                     /locus_tag="ACG81A_000745"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFS5014136.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            13262..14383
                     /gene="dinB"
                     /locus_tag="ACG81A_000746"
     CDS             13262..14383
                     /gene="dinB"
                     /locus_tag="ACG81A_000746"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFS5014137.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(14437..15039)
                     /gene="nrdG"
                     /locus_tag="ACG81A_000747"
     CDS             complement(14437..15039)
                     /gene="nrdG"
                     /locus_tag="ACG81A_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFS5014138.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(15354..17543)
                     /gene="nrdD"
                     /locus_tag="ACG81A_000748"
     CDS             complement(15354..17543)
                     /gene="nrdD"
                     /locus_tag="ACG81A_000748"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFS5014139.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
BASE COUNT         5585 a         3292 c         3637 g         5203 t
ORIGIN      
        1 agattatttt cagatctgaa cggttttttt tatctattca tcaagatcaa atttcatttt
       61 tcaatgattc aatcaattcg ccctctgctt catgactatg ttttcggcca ttaacaagat
      121 agagtgctgt acatatcaat ggaatgactc cggcaatcac atacaatcct ctaaatgaag
      181 taagagattt tagcatcccc atcaaataag gaccaacgcc taagcctagg tctaaaccaa
      241 taaagtaggt agataaagca atactgattc gatgttcgcg gacgattttt aaacaaacag
      301 cctgtccatt tgacataaac gtaccataac ctaggccgac taatgctcca gagataagca
      361 atgtcacact attcgtcgtt acactcagta aaaataatcc tgctgttaag aataaatagc
      421 taggatacat cacgtattgt tctcctttga catcgaaaat ccttccagtc aatggacgtg
      481 taaatgtgat gaccaaagca tatacgacga agaaaaatga cccagcagca actaagtgga
      541 ttacttttgt ataagaagat aaaaatgata aaacacttga ataagaaaga cccatcaaaa
      601 aggcaattcc tgtaatgaaa agtgctttct tttcaataaa actattgata ttccatgagc
      661 gaagagatat tttttgttct tcagataatg gaatattttt tacaggaaag acaaggcagg
      721 caatcgtaac aaagaagacc agtacaattg aaaaccagat aatgaaacga aaatcagtca
      781 tattcagcaa aatcattcct aaaaatggcc caacagcggc agctaaacta gtacttaatc
      841 cataataatt gatgccttcc ccttttttag atgcagggat atatgcagta acgatagcat
      901 ttgtagctgt agaaactgta ccgtagccga atccattcaa gaatcgaact aaatacaatg
      961 ccccgatagt tggcgtataa agatatgcta atgttgaaag gagatagaag atcgcgccgc
     1021 ctcttaaggt aaacttccga ccaaatagtt ctaactgttt ccccatgaat aagcgtgcca
     1081 gcaatgttcc gatgatatag atcccagaag caaaacctgc ttggctcaag ctggcgtgta
     1141 aggtgtcttg cgcaatgact gccatgatga ccatcaacag ataataaatc aggtagacaa
     1201 taaaattgat caatgtgatt ccaataaaat atttattaaa taatttcttt tccatgtatg
     1261 ttatacattc cccttaagct gaatatgctt ttaatttttg tatttgaact tctacatcgt
     1321 gataattgga tggatagcga tcaacaagtt ggcgaataaa taaatctctt gtagacataa
     1381 acagatttaa aaattcttcg cattcgtttg ttggattctc atccactgct ttgatcaatt
     1441 cgatcgagtt ttgatacgca tcatttaaaa agactaaaga agctttcata tcaaatcttg
     1501 tttgatatgc tgtttctaat cgattaatga ttgtgttcat ccagtaattc atgttttttc
     1561 cctctattct taaaaaattt tacttctgca gtataacgaa taaaaaaaag gaaaaaaatc
     1621 gcataagtga tggaaaaagg tacttaacaa atgttaatat cattagtaaa atacctttgg
     1681 actttaaaaa gttttaaagt actgtatgat ctttttagat gaaagtttgg atgggggagc
     1741 atcattggat agtagagtct taaaggaata tttggataaa aatgattttc cgattatacg
     1801 caaaggatat cataaatatt tgacattcga agggatggaa gattcccata cgtatatctt
     1861 gaaagaggga acggtaaaag caagtgttat ttcaaatgat gggagagaat ttaaccttcg
     1921 ctatatcaaa gatcttgaaa tcgtctctct tttaaaagac gaatattcac aattcatcga
     1981 ttcgccgtat aatattcgag ttgaatcaga atatgcagag ttttatcgaa tcaatcgtgt
     2041 gcgcttttgg caaaatatca accaagaccc aaagctacaa cactatgtaa aagagtatta
     2101 ccgctatcgt ctgctttatt cgatgaaaaa aatgcagcaa atgctattaa acggaaagct
     2161 gggagctgtt tgtacgcaac tttacgaatt atatgattta ttcggtgtgc ggacagaaga
     2221 agggatgctg atcgattttt cgatcacgaa tgaagaaatc gccaaatttt gtggcatctc
     2281 aactgctagc agtgtaaaca gaattttaaa acaactgaaa aaagatggta ttatcaccgt
     2341 taaagaccat catatcgtta ttttgaatat ggacttgtta gaagacaata ttatcttttg
     2401 atgaacaaag aggttgtgaa atcagcgttt tgacctgagt attagagaaa caaacggaaa
     2461 aatagttcct catattttgc cgttttttga gctaataccg agggtcagct gattgaacac
     2521 cgtttaccaa gaggttgtga gcctgccgtt taactctgag tcacaaggaa caatttcaaa
     2581 aaacagcttt tcatgtttga tgaatattgt gacttgtgac cgaggagttg gtaggagaac
     2641 accgtttacc aagaggttgt gaaaaagctg ttctgcatca agtaataaga agagccaagt
     2701 aaaaacagtc tcttatgttt ttaacttggc tggacttatc accgtagatg taagcttttg
     2761 aacgccgttt atttgcaaca aaaggggctg tgacacgatt tttgtcacag cccttttttt
     2821 gcgaacattc atttagcaat ttttagaaac ttctgccgcc accgccaaaa gtcccgccac
     2881 cgctagaatg ggtagtactt ccgccaccgc cgcttccagg aggtgggctt ttggggattc
     2941 ttcttgtcgt gacaaaagaa tttgtcagac gatcggtttt ttcagttaat ttgatactgg
     3001 atttttctcg gaatggatat ttgtagatgc ctgatttcaa ttgataacgc gaaacagtca
     3061 caaaataaaa gacaagacta aggataagag ccaaaacaac agcaatgatg atttctactg
     3121 tcgtcaaaac cttatagcgt gtaattttgc cagtttcttc gtctacacga taatgtcctc
     3181 ctggaactcc gtcttccaca tatgttgctg atttagacag gtaagctttt gcagcattgt
     3241 aataggcagc atttgtcatc tgatcataaa catcatctaa gatcgaatcg attctgctat
     3301 cagtcaaata atcgatcata ttaccagaag tcgaaatgta gatttcacgc tgtcccatat
     3361 ctaatagcaa tactgagcca ttttgatcat ttccgactgc gtctcttaag taattatcag
     3421 caaagtctct tggttcttct gaattagatg tagtcgttac gatcaagact cgtcctttga
     3481 ttttttcatt cagcttttct gcctgttgtt ccaaagattg gatttgatct gttgtgaata
     3541 acccagcctc atcatttacc gtagctgtat ctgcatgagt agccaccggc cagccaacac
     3601 ttactagcaa aagaaagaat aaaaaaaact ttttcatcat atcaagtaac ctccgatcag
     3661 aaagaggatc gctagcccag cgaaaatccc aagagcggtc agtcccaatt ttttctggct
     3721 gatcggcagg acaccgctga cctttcctgt ttgtccattc attgcgtagt agtagacttt
     3781 tttacttgag tcattgcttc gataagtaac aagccatacc ggcagtaata cgtaattatt
     3841 tacttcgttt gtgattgaag cagaagttcg cacaccggtc aatgttgtat atccatttgc
     3901 ggtatctttt aacagcttct ctgaatagtc ttttaattca gattgaatct gtgatttgat
     3961 tgcctcatat tcgatatctc ttttttctgc ttggaacccg gctaaatact ggcttttaaa
     4021 atcaattgct ttatctaacg ggaaaggttg cacgccttcg accatctttt gttgcgtatt
     4081 ttttgataag gcatttttta ctagttcttt aaaagaaagc ttgcctgctc ttgaaacagc
     4141 aaattgcttc gtttctgtgt attcgatgtc acctatccgc catatgcgga taactgtacc
     4201 attcgcctgc atggcaccat ctacttcagc atctgtagcc caataaggga aatacacacc
     4261 tgtcaactta tcgatttgat ccttattgaa aaaagctttg ggaacaaacc attttttctt
     4321 ggtccaagcg agaaatctgt tgattgcttc ttctttctcg acagcaaaag gaagtacttt
     4381 atttggcaag aattctccgc ttaaacgtcc cgatagtacg acgggattat ggcagtagta
     4441 acaataagtc gcagcagttg tcgcatcagt cacgatctct gcaccacagt tgggacaaag
     4501 aaaaagatct atcgctgcgg tattatctgc agattcgtcc gctgtatctg aaaaaccgcc
     4561 tgcctcgtca accgctttgc gctcggcttc attcatatcg gcaagctgtt cttgggctgt
     4621 aaaagtaaaa tcttcggtag gtgaaggttc ttctttgctt tcttcgccag ccatacgcgc
     4681 ttctttttgc ttttgctcat attggctgac ttcttcttct gtatagatat tcaggcagta
     4741 ttcgcaatga aatttttggt ctttgggatc aaaagtcaac ggaccgccgc aattaggaca
     4801 tttatgtgta agaacatcca tcagctgcac acctttctaa ataaattaat taacggggat
     4861 tcctttaaac ggtttaccac agtttggaca gaatttaggg atgccgttgg aaagatccac
     4921 gatctcattg cattcactgc atcgcatttg tagcttaggc tgaacgtttt ctacaggttt
     4981 ggcagctccg cagttggaac agaattttcc ggaattttct gtcccgcaaa ctggacatgt
     5041 ccaagtatcc gcatttcctt gtgcagtttg ttgattttgt ttttctgctt gttgtttgat
     5101 ttgttcttgg ttattttgag ctgcttgaga aagataagaa ccatttgcat tcattcccat
     5161 acctacaccc ataaagccgg tcattgctcc tgcttcattt tttccggctg cttccatacc
     5221 acgtgcaatg gaaccttgca cgtatccttc acgtacgcta ggatcaccta gcatcgcccc
     5281 ttcgttacgc atattgatca gtttcacaga atcatcggtg taagaaatgc tggctacagc
     5341 cacagaaaca atctccatcc cacgtaattc acgccaagaa tcatccaatg cagtatccat
     5401 gtatttgctt aattccaaac tcttagatgg tacataagaa atccgttgac catctgcaga
     5461 catctgattg attgctgatt gtagcgctgt taggaattca gctaaatact gctcattgat
     5521 atcattgatt tccacttgtg ttttgttttt agggattgcg tttgtataaa ataaaatcgg
     5581 gtcaacgaca tgaatagaat aagtgccatg tgcccgtaag aataattctg cgttatagaa
     5641 attgtcaaag tagttcaacg gcgcgcttgt accgaatttg attcccttta tttcttggag
     5701 attgatataa aaaacttgtt gtttctgcgg tgtgacgccg ccaaatttga atcggtcaaa
     5761 agtttcagca attgcttctt tcagtgttcc attgaacatg gaaggagctg cgtcattttt
     5821 aatggtgtaa taaccttcct cagcggtata atcgatgatt tttccaccat cgaccaaaag
     5881 catcatcatg tttggataga catggacgac tgttccgtct gttaaaacat cttctgttcc
     5941 tttacgattg gagccacgtt tgtcatcttt gcggactttg acaccttttg tcatgaccgt
     6001 tgtatcactc atattgtctg gttcgatgac ttctagccat tgatctgcca gaccgccgcc
     6061 aaccatactt gttgctgctt tgataagacc cataaaaaat tcctcctctg attcagctag
     6121 tgccaaacaa ttattgttta tttattatac cataggaaga tgtgaaactt ctcggactag
     6181 agacggaatt gaaatggaaa attgcgtatt gtttaaatac ctgaaaaagc cgagaaggat
     6241 agtagttttt ttacctaata tcacgtataa taagagatag tgaatttttt aaaaaagggg
     6301 gatccttatg gcatatcaag cactttatcg agtctggcgt tcgcagcgtt tcgaagacat
     6361 cgttggacaa aaagctgtga cacagacact aaaaaatgct attgtttccc acaaaacctc
     6421 acatgcctac ttgtttacag gacccagagg aaccggaaag acgagtgcag caaagatttt
     6481 cgcaaaagca atcaactgtc cgaacagtaa agacggtgag ccatgcaacg aatgcgaaat
     6541 gtgtcgatca attactgctg gtacgcaaga agatgtgatc gaaatcgatg ctgccagcaa
     6601 taacggagtc gaagaaatcc gatttatccg tgatcgggca aattatgcgc ctacaaaggc
     6661 agcgtacaaa gtgtacatta ttgatgaggt acatatgctt tcaacaggag ctttcaatgc
     6721 cttgttgaaa acattggaag agccaaaaga aagtgtcata ttcatcttag caacgaccga
     6781 acctcacaaa ataccagcta cgatcatttc acggactcag cgatttgatt ttaaacggat
     6841 caatacggca gatattgtcg aacatcttga atttatcttg aaagaatcga ctatcagcta
     6901 tgaaccagct gcattgaatg tgattgcccg ttctgcagaa ggcgggatgc gggatgcatt
     6961 gagtattctt gatcaggcaa tctcgtttag tgaaggaaca attactttag atgatgccat
     7021 gcaagtgaca ggtagcttaa ctgacgaaat gatggacagt tacttgagtg cttgcgtgaa
     7081 aaaagaagta ccagaagcat tggaaacatt gaaccaaatt ctcgcagccg gcaaagaatc
     7141 acgtcgcttt ctagaggata tgttacaata ctgccgtgac ttgctgatgt atcagcaagc
     7201 acctaaccta gttgaggaac aatcgagtac gatcagaagt acatttacag aattggcaca
     7261 aactgtttca gcggatcagc tgttttcaat gataaagatt ttgagcgata ctcaaaatga
     7321 aattcgtttt accaatagtg ccgctattta tttggaagtc gctacagtca aacttgcaca
     7381 gctaccgtct gcttctgttc cttcagcaga tgtagcattt gcagatgatc agatagtaaa
     7441 acagctacaa caagagatta aggaactaag aaacgaaatc aaggaactac gcagtaatac
     7501 aggaattgag aatacaaaag tacagccggt acagcaaaca aatcataaaa aacagacaag
     7561 tacctatcgt gtaccgacgg aacgagtata taaagttctg cgggaagcga caagacagca
     7621 tctgaaccaa gtaaaagaag tatgggaaga cttgctgatg agtctatctg tcactcagcg
     7681 agcaatgctt aaagcaagcg aaccagttgc agctggacct acaggactag tgattgcgtt
     7741 tgattatgag attgtttgtc aaagagcagc aaatgacgaa gaattgcagc tttcggtaca
     7801 caacaactta agccgaatga tcaaagatta tgcgcctgat cctgtttata ttacaagaga
     7861 aagctggcca gaacttcgaa gaaattattt gacgcaggca caagaagagg gaacagaaaa
     7921 ctcttttgct tctgaagact cagaaggaga aatcgagttg cttcctcaag aacaagaagt
     7981 accagaagaa acacttgtag taaaagcaga ggaactgttt ggctccgaag ctgttacagt
     8041 ggtagatgat taataaaaag gagtagataa ttatgatgcg tggaatgggc aatatgcaag
     8101 gcatgatgaa acaagtacaa aaaatgcaaa aagaaatggc agaagcacaa gagctgctaa
     8161 ataaaacaga gtttacaggt aaagccacca atgaattggt caaagcggtt tttacaggcg
     8221 atcgtcgcat gaaagatatc caaatcaatg aagaagtcgt tgatcctgat gatatcgaga
     8281 tgcttcagga tcttgtcgta atggcagtca atgatgcgat cgcacaaatt gatgtggaaa
     8341 ctgagaaaaa attagggaaa tacgcaaaag gtttgccagg attttagttt taaactgaag
     8401 gagtaatgaa tatgcaatat ccagaaccaa ttgcgaaact gatcgacagc tacatgaaac
     8461 ttccaggaat cggtcagaaa acagcgacaa gactcgcctt ttatacgatc gatatgaaag
     8521 atgaagtagt aaatgaattt gctaaatctt tgttaagtgt aaaacgcgat ttgcattttt
     8581 gtagtatctg cggaaacatc acagaagaag atccctgcga aatatgtaga aatccttcac
     8641 gtgatcgaag cgttatccta gtggtcgaag agccgaaaga tgtaatggca atggaaaaaa
     8701 tgcgagaata tcacggactt tatcatgttc tccatggtgt gttatcaccg atggaaggga
     8761 caggtccaga agacatcaat atttcttctc tgttgcagcg gctgcatgat gcagatgtaa
     8821 aagaagtgat tatcgcaaca aatgcaacaa cagagggaga agcaacagct atgtatctct
     8881 cacgtctgat caaacccgca ggtatcaaag tgactcgtct agcacatggt ctgtctgtag
     8941 gctctgatat cgagtatgca gatgaagtta ctttattgaa agctgtcgaa ggaagacgtg
     9001 agttataaat ttctaattaa gtgttttttc agatacaata taaaagcagc acaagagatg
     9061 ggggcaacaa tgtgaacgga ctatttatta caatcgaggg gcctgatggt gcaggaaaaa
     9121 ccagcatctt gaatgaactt taccctcttt tgaaagaagt agcaaaaaca gaaatcattc
     9181 aaacaagaga accaggaggc attccgattg ctgaagagat ccgagcagtg atccttgatc
     9241 caaaaaatga tcggatggat gaacgtacgg aagcactgct ttatgcagca gcaagaagac
     9301 aacatctagt cgaaaaggtt ctgcctgctc tggcaaaagg gaaaatcgtt ttatgcgata
     9361 gatttgtcga cagttctctt gcttatcaag gtgcaggccg acgcatcggc gttaccgaga
     9421 ttgctcggtt gaatgagttt gctacagaag gtactacgcc ggattttacc ctttatcttg
     9481 acgtagattc agacaccgga ttaagaagaa tcaagaaaaa cagacaaaat caaatcgacc
     9541 gtttagattc ggaaggtttg gaattccacc agcgtgtacg tcatgcttac ttaaagctgg
     9601 cagaagagaa tccggaaaga atccacaaag ttgatgcaag aaaaagcttt gaagaagtgc
     9661 tgcaaacaag ttatcataca attattgaac agtatccgca attttttgaa aactaggaag
     9721 gtgaacgttt atgaaaatta ttttagcgat tatccaagac aaagacagca accgtttatc
     9781 aaatgaattg attgatgcaa atatccgtgc cacaaaacta tcatcgacag gcggcttcct
     9841 taaggctgga aacagtacgt ttatcgtggg tattgaagat gaccgtgtag aagaagcatt
     9901 ggaaatcatc aaaaaaacgt gtgaatcaag aaagcaattt gtatctacac ctgttacact
     9961 ggatatttcg atggatgggg gcgtccctta tccagtagaa gtagaagtag gcggtgccac
    10021 agtcttcgtc ttacctgttg aaggattcca tcaattttag gaggagctga gctatgaatc
    10081 aagaaataaa cttggctcaa atgcaaccga tcgtgtatca gcagcttcaa cggagttttg
    10141 agcatgggcg tatcgctcat gcttatcttt ttgaagggga gaaaggaaca ggaaaacatg
    10201 aagtaggtat atggcttgca cagcacctgt tctgtaccca aatgaaagac aatctgcctt
    10261 gtggaaaatg caacaattgt cagcggatcg aaaagaaaga acatccagat gttttggtta
    10321 ttgaaccgga aggacaaacc atcaaagtcg atcagatcag acgtttgcag acggaattca
    10381 gtcgcagcgg ttacgaatca agaaaaaaag tttttttgat caaagaagct gaaaaaatga
    10441 atagcagtgc ggcaaacagt ttgttgaaat tcttggaaga gccccctgga gattttttag
    10501 cggtgttaga aacagatgca gtaggaagaa ttctccctac tattcaatct cgttgtcaag
    10561 ttctgcattt tcaagaactt tcgaaagaag cactgatcca tcgattgcaa gaagagcaga
    10621 ttccattgga aaaagcaaaa cttttggctt tcttaacaaa cagtttagga aaagcagttg
    10681 aaatctctca agatgaatgg tttaatgatg ctaaggactt gatttatcaa tggttcgttt
    10741 acttgcaaaa gcaagatact caagccttta tctatgtcca aaaaaaattg gtaaaatcat
    10801 ttaaagaaaa aacacaacag tttacaggat tatctgtctt gatgttttat tatcaggaag
    10861 cacagcgtca agcagtagca aacggcgaat atggcaaagt gaaacagata aaccagataa
    10921 ttgaacggat attgatcgca gagcagaaac tacgcagcaa tgtcagtttt caggctgttg
    10981 cggaacagtt cgttttgcat acgatttttt agcttgccgc aaaggatgat gatcaaggag
    11041 gatggaaatg gtagaagtag taggtgtccg atatagagat gcaggtcata tctattattt
    11101 tgcaccagga gactctgaat atgtttatag tgacaaggta ttagtcgaat ctcagcaatc
    11161 cttacatatt gctactgttg tccttcctaa aaaagagatt gatcccaatg accttcctga
    11221 agctatccgt ccgatcttgc ataaagcgtc acaagctgaa ttagaaaaag acaaaaaaaa
    11281 caaaaaagat gcttatgatg cattgaaaat cgcaaaagaa aaagtgcagt tgcgtgagtt
    11341 ggaaatgaaa atggtcaaag ccgaatacac atttgatcga aataaattga ttttttcttt
    11401 cacagcggac gggcgaattg actttagaga attggtaaaa gatctggccg ctgtatttcg
    11461 tactcgtatt gagcttcgac aaattggtgt ccgcgatgaa gcaaagataa taggcggaat
    11521 tggtccatgt gggcgtccat tatgctgttc ttctttttta ggcgatttcg tgcctgtatc
    11581 aatcaaaatg gcaaaagatc aaagtttatc tttgaatcca acaaaaattt ccggtttatg
    11641 cggacgcttg atgtgctgtc tgcaatatga gagcgaagaa tacgagacag cgaaaaaaga
    11701 actccctgac tttggaaaag taattgctac acctgatggt aaagggaaag tcgtagggtt
    11761 gaatctgctt agccgtatcg ttagtattcg gctgactgga cgggaaaatc ctgtcgaata
    11821 tgaatgggat gaattgaaag aatttatcca agtaggtgag atgaatggat aagagatcat
    11881 tatacgacgg attgaatcaa ttagaatcag agttgagaaa tacattaaca gaacttgttg
    11941 aaatgaagca tgctttgcat gagatcgtag aaaaaaatac aactttggag atggaaaatc
    12001 aacgtctgcg ggaacacctg cgggaattga accaagttgc acaagctcca gctgaaaatg
    12061 tgaagcagga actttctaag tctcgtatga atcttgaaaa aatttatgaa gaaggattcc
    12121 atgtctgcta cgatttatat ggatcaagaa gggacaatga cgagccttgt gcgttttgtc
    12181 tagaagtgat ctatcgcgaa agcggtcgat gaccaataac aagaggagac tggagcggag
    12241 aaatactgct ccagtctttt tattaaggag attgtgacag atgtataatc aaaaaagttt
    12301 caagggaaat ccggtatatg gggatttata tcttgtaccg acgccaattg gaaatttaga
    12361 agatatgagc tacagaagtg ttcgtatgct gcaagaatcc gatcttatcg caagtgagga
    12421 tacgcgtaat acacaaaagt tattgaatca ttttgaaatc catacacccc aaaaaagttt
    12481 acatgaacat aattataaag aacgtgtccc tcaattgatt gctttgttaa aagaaggaaa
    12541 aacgattgct caagtcagtg acgcaggaat gccttccatc agcgatcctg gacatgagct
    12601 agttttagcc tgcataaaag aagggatttc tgtgatctct atcccaggac ctacagctgg
    12661 gatgacggct ttgattgctt cagggctagt gcctcaacca tttttatttt atggcttcct
    12721 tccacgtaag aaaaaagaac agaaagaaac attgaaaaag ctaaaagaac agacagttac
    12781 gctgattttt tatgagtcac cttatcggat cactgctaca ttgacgaatt ttttagaaat
    12841 ttttggaaat cgccaagtcg ttttgtgtcg ggaattgaca aaggtccatg aagaatacct
    12901 tagaggaagt acggaagagc tactcgctta tatcgaagaa catccagtaa agggtgaatg
    12961 ttgtctgctt gtagaaggga atttgttcga aatgccagtg acggaagttg atgaagagtt
    13021 gggaacgtta aaggaacaag tagaagcaaa gatcgcttca ggcgaaaaac caaatgctgc
    13081 tatcaaagca gtagcgctga aaaatgggct gaaaaaacaa gaagtctacc gtcagtacca
    13141 tgaattagac tgagatagta taagttgtct ataatgaaag actaatgagg aaatcagcta
    13201 ctaacttttc aaatctcttg aacgcgaacg aatgttcttg tataatgaat aagaggtgag
    13261 gatgatggag ctgcaatttc ctttacataa tgatacatca agaaaaatca ttcatattga
    13321 tatggatgct tttttcgctt cggttgaaga acgagatcat cctgagtttc gcggacatcc
    13381 attagtaatt gccagacacc ccagtgacac tggtgggcgt ggagtagtga ctacagcaaa
    13441 ttatgaagca agaaaattcg gaattcattc agcaatgagt gcacaaaaag cgtatgagct
    13501 ttgtccgcaa gccatcttta agcctggcga ccatcataaa tacagcgata tttccaaaca
    13561 agtacgggaa gtattctatc gttatacaga tattgtggaa ccagttagta tcgatgaagc
    13621 gtatcttgat gtgaccgaaa ataaaatcaa ttgcaagtca gccatcaaaa tcgcccgaat
    13681 gatccaagca gatatttggg agagtgtcca gctaacatgt tctgcgggag taagttacaa
    13741 taagtttttg gccaaattag catcagacta tcaaaaacct agaggaatca cggtagttgc
    13801 ccctcaagac gctgtgccat ttttaaaagc tttgccaatt gaaaaatttc atggggtagg
    13861 gaagaaaact gtacctagaa tgcatgagct agggatttat acaggaaaag acctatatga
    13921 atgcacagaa atgatgttga ttcgaaattt tggaaaaatg ggctattctc tttatcgtaa
    13981 agtcagagga atccatgatt ctccagtaaa cgttacaaga gagcgaaaat ctgtgggaaa
    14041 agagcatact tatgggcagc cgttgcaaac ggaagaagca gtgctgactc agttgcgcca
    14101 actagctgaa aaagtagaag aagcactgag aagagtccaa aaacatggga aaacagtagt
    14161 tttgaaggtc aggtacacgg actattctac tgtgaccaaa agagtgacat tgccagaata
    14221 tatctataaa aaagaagcac tcttttatca agcaagcctt atatgggagg aaatcttggg
    14281 agtagaaaaa ggaatccgat tgttagggat tactttgacg aatctggacc cgatgactta
    14341 tgaaaacatc gtccttccat tatgggaaaa tcaagaaata taaaaacagc tttgagagtt
    14401 gaaaccggaa atccgttttc tctcccaaag ctgtttttat atcaagtctt gctttttgat
    14461 ttgttcatag gtttctgttg catccgtaca tttttcccaa atcacaactt gattggattc
    14521 taaagatttt tggacgtcaa tgatccgctg gttgctactt ccgcgaaatt gtagttttag
    14581 gtcacgtttg cttaattcaa agcgtccgtc aacaaggata tcgatttgcg aaaggagttc
    14641 taatttatct tctgaatctt gtagcaattc atcaaaggca tagcctgtcc atgaccagat
    14701 atcttttgtc gtaccgaact cttttcggat acgtcgtacg acttgcagac aaacatctgt
    14761 attcaaaaaa ggttcgccac caagtaaagt taatccttgc acgtaatcgt tgcgcaagtc
    14821 agcgatgatt tgttcttcta attcaggtgt gaatggtttg ccatatcgga aattttgtgc
    14881 agctttgttg aagcagcctt cgcaggcaaa taagcagcca ctaacgtaaa gactgttgcg
    14941 gacaccttca ccatcgacaa aattaaacgg tttgtagtca gcaatatagt tttggctgta
    15001 gtcttttgct aaccattctt ttggttttgg atttctcata aaaaaacttc tttcatttaa
    15061 ggttcaaaga tatagctatt gaagataaaa tctactcgac cacataaata agaacaaact
    15121 gacctaaatc cattgttcaa gaaagcttgt tcttattttg atagagggcg ttcaaagata
    15181 caaagccatc gaaagagggt ggtgaaaaaa gacttgtcat agcctcttat tctgaataaa
    15241 cggtgttcaa aagcttgcat ctgcagtaat aagcccaact aaacgaaaaa tatgaggaac
    15301 tatttttgtt tagctattct tattacttga tgcaggacag ctttttcaca acctcatttc
    15361 atgtgtttca cacgtgagga aatttcttta tggcggccat gtaccatcgg acgagcttgc
    15421 ggatttccta aatagccaca agtacgtttg actacatcac atgtttttgg atcatgattt
    15481 ccacattctg gacattcgaa ccctcgttct gtcggtttga aatcaccttc gaaaccacat
    15541 tgatagcaat gatcgattgg cgtattcgta ccaagatacc cgattttgtc atatgcaaaa
    15601 tcccatacag cttctaaagc tttcggattt tgttggagca tagggtattc acagtagtgg
    15661 atgaatccac ctgaacaata ttttggataa tccttctcaa attccaattt ttcaaatggc
    15721 gttgggtttt tacgcacatc atagtggaaa ctgtttgtat aatattcttt atctgtgata
    15781 ttttcgatga tgccgaattt ttctgtatcc aaacggcaga aacggtcagt tagactttca
    15841 cttggagttg aatagacact gaaatgataa ccatattcat ccccccatgc atctgcatta
    15901 gcttttaatt ctttgacgat atcaagtgtg aaagcttttg cttctgggtt tgtttcccat
    15961 tcgccaccga agaaagctga agcaacttca tacaatccaa tgtagcctaa cgatacagta
    16021 gctcgacggt ttttgaataa ttcattgaca gaatcggtac gtgaaagacg tttcccgaaa
    16081 gaaccataca tatataaaat tggcgcattt gctggaatgg cttctttaca acgttctaca
    16141 cgatagagaa gcgcatcttt catgatttct aagcggtcag acagtaactg ccagaattta
    16201 ttctgatctc cttttgattc gatcgcgatt cttggcaaat tcaatgtaac aacacctaaa
    16261 ttcattcgtc cgacatttac ttcttgacca ttttcgtctt tccacccttg caagaatgaa
    16321 cgacagccca taggtacttt gaaacttcca gttaattcaa cgattttatc gtaattcaga
    16381 atatcaggat acattctttt cgttgcacat tccaatgcta gttgtttgat gtcgtaatta
    16441 ggatcttcag ggtttaagtt cacacctttt ttcaaagtga agatcagttt agggaagata
    16501 gctgtccgct tttcactccc taatccattg attcgaatct gtaaaattgc tttttgaatt
    16561 tctctttcaa accagttttc tcctagtcca aatcctaatg aagtgaaagg agtttgtcca
    16621 tttgaagtaa ataaggtatt gatttcatat tccaaacttt gcatagcatc gaagatatct
    16681 tttttggttt ttgcttttgc atattctttt tggcgttctg gcgaatcgat ccattcttcc
    16741 gcgtctttta gatgtttttt atagttcaat tctgcaaaag gagcaagcaa ttcatctgtt
    16801 cggtcggcag aacagccacc atattgactt gacgctacat tggcaataat ctgtgagatt
    16861 tgtgctgtgg cggtctggat agattttggc gattcgactt cggcattgcc gattttgaac
    16921 ccattgttca gcatgccttt gaaatcaatc aagcagcaat tcgtcatcgg tgtataagga
    16981 tgataatcca aatcatggta atggatatcg cctttttgat gcgcatttgc tatatgtggt
    17041 ggcaacattt tcagtccgat tgattttcca acgatcccgg ctgtcagatc tcgctgtgta
    17101 ttgaaaacat tactgtcttt atttgcgttt tcgttcacga cagtttggtc cttgttgatt
    17161 agcttttcaa tcgaaaagtt gatatctgtt gctttactgc gagcaaaatc tcgtttggta
    17221 cgataattga tgtactcttc tgctaattca tattcgtttt tttctagtaa tatatgttcc
    17281 acgatgtttt gaatctcata gattttgaca ttttcagcaa accggctttt gatttcttgg
    17341 acaaccaagt ctgcaatcat ttgaattttt tcataagtaa gtggtgataa tgagccgtga
    17401 attttctttt ctgctttgat cagtgcttca ttgatctttt gttcatcgaa atcaacatgg
    17461 cggccatcgc gtttgatgac tttcatctgg gatgtctctg gctgctgagt aacgacagtt
    17521 ttttcttcat aatttacttt cattatcttc aactcctgct aaattgttct tgtctatcat
    17581 aaaacaaaat cggggaagga gcaactaaat atcggctata tatagtattc aatgtgctgt
    17641 ataattggat ttctatacta tatgtagtgt atattttatt gtgaaaaaag gtgaaaactt
    17701 gtagctggtg gatttgt
//
LOCUS       DAZULY010000033        17437 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000033 DAZULY010000000
VERSION     DAZULY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.033"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..664)
                     /gene="rpsD"
                     /locus_tag="ACG81A_000749"
     CDS             complement(53..664)
                     /gene="rpsD"
                     /locus_tag="ACG81A_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFS5014140.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            926..1777
                     /locus_tag="ACG81A_000750"
     CDS             926..1777
                     /locus_tag="ACG81A_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFS5014141.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            1805..2440
                     /locus_tag="ACG81A_000751"
     CDS             1805..2440
                     /locus_tag="ACG81A_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFS5014142.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            2460..3233
                     /locus_tag="ACG81A_000752"
     CDS             2460..3233
                     /locus_tag="ACG81A_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5014143.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            3641..4438
                     /locus_tag="ACG81A_000753"
     CDS             3641..4438
                     /locus_tag="ACG81A_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFS5014144.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            4416..4829
                     /locus_tag="ACG81A_000754"
     CDS             4416..4829
                     /locus_tag="ACG81A_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS5014145.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            4813..7458
                     /gene="mutS"
                     /locus_tag="ACG81A_000755"
     CDS             4813..7458
                     /gene="mutS"
                     /locus_tag="ACG81A_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS5014146.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            7476..9584
                     /gene="mutL"
                     /locus_tag="ACG81A_000756"
     CDS             7476..9584
                     /gene="mutL"
                     /locus_tag="ACG81A_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFS5014147.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            9606..10166
                     /locus_tag="ACG81A_000757"
     CDS             9606..10166
                     /locus_tag="ACG81A_000757"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFS5014148.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(10251..12239)
                     /gene="treP"
                     /locus_tag="ACG81A_000758"
     CDS             complement(10251..12239)
                     /gene="treP"
                     /locus_tag="ACG81A_000758"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFS5014149.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            12462..13175
                     /gene="treR"
                     /locus_tag="ACG81A_000759"
     CDS             12462..13175
                     /gene="treR"
                     /locus_tag="ACG81A_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFS5014150.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            13252..13698
                     /gene="msrB"
                     /locus_tag="ACG81A_000760"
     CDS             13252..13698
                     /gene="msrB"
                     /locus_tag="ACG81A_000760"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFS5014151.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            13868..14470
                     /gene="ruvA"
                     /locus_tag="ACG81A_000761"
     CDS             13868..14470
                     /gene="ruvA"
                     /locus_tag="ACG81A_000761"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFS5014152.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            14483..15484
                     /gene="ruvB"
                     /locus_tag="ACG81A_000762"
     CDS             14483..15484
                     /gene="ruvB"
                     /locus_tag="ACG81A_000762"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFS5014153.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            15513..16091
                     /locus_tag="ACG81A_000763"
     CDS             15513..16091
                     /locus_tag="ACG81A_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5014154.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            16143..16625
                     /locus_tag="ACG81A_000764"
     CDS             16143..16625
                     /locus_tag="ACG81A_000764"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFS5014155.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            16742..17116
                     /locus_tag="ACG81A_000765"
     CDS             16742..17116
                     /locus_tag="ACG81A_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014156.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         5786 a         3114 c         3818 g         4719 t
ORIGIN      
        1 aaaagaccag ctatccaata gatagcggtc tttttcaaga aagcttttga aattataagc
       61 tttggttgta gtattcaacg acaagagctt cgtcgatttc tggatatagt tcgtcacgtt
      121 ctggtaaacg agtcaatgaa ccttccaatt tttcagcgtc aaagcttacg aatgctgggc
      181 gtccaacagt tgcttctaca gcttctttta ctgttacgat gttttgtgat ttttcacgaa
      241 ctgagatcac ttgacccact tctacgtggt atgaagggat atcaacgcgt ttgccatcaa
      301 ctaaaatgtg accatggtta actaattgac gtgcttgacg acgagtcgtt gcaagtccta
      361 aacggtaaac aacattgtct aaacgttgtt ctagtaagat catgaagtta acaccatgtt
      421 taccttcttt gattttgcta gcttttacga acaagttacg gaattgacgt tcgttcattc
      481 cgtacatgtg acgaagtttt tgtttttcag ttaattgcat gccgtattct gattgtttgc
      541 cacggctatt aggtccatgt tgacctggtg cgtaagggcg acgtgctaat tcttttcctg
      601 tgcctgacaa tgagatgcct aagcgacgag aaattttcca tgatggtcct gtataacgag
      661 acattaaaat ttcctccaat gaatataagt ttttggagta aaataatccg ttgaaaaatt
      721 cataatcata tagtttgttc ttcaatcttc acctttgcag ccgcggttac acaatcgaac
      781 ccgagacggg gcaacaaact agggatgagt tattattttt ctgctgcatt attttacaca
      841 aattacagta tatgatagat catccacgaa tgtcaagata attagtacat aaaaacgctt
      901 tacaaaccta ggctgtcttt ataaaatgag agggtatgtg caaatggaaa aaagcgaagg
      961 aagtttaagg agaaaatcaa tgaatccggt atcaccatta tttgagaaaa tcgatgcttc
     1021 aattgaaaag aaggcgaatc tttttgaaaa cagcttttca cgttacgctg ttcgagcggt
     1081 tctcgcctgt ttgtttttga ctttaggaac ggctgtggca tttgcgatcg cgatgaaggg
     1141 ggaagatatc gcgcatggtc taggaaagat tttatacgca tttatgttca gctggggtct
     1201 ggtcatgatt ctatacatga acgccgaatt aggaacatcg aatatgcttt atatgaccgt
     1261 gggtgttttt cggcagagac ttgctttaag taaggcagcc aagattctat ttgcctgtat
     1321 ttttttcaat atgattggcg gtatattatg tggatttcta atttctatga ctacgccatt
     1381 tcaagattta acgttggaca actttatgtt agaatcaata gcagtaaaac tagcaaaacc
     1441 ttcagcaacg attttggtgg aagggatgtt tgcgaatatc gttgtcaata ccgctgtact
     1501 gatcagcatg cggatgaaag aggatgtcgg aaaagtgatc acagtcgtat tcatcatttt
     1561 catttttgct tttttaggat atgaacacgt gatagcgaat tttcctgcct ttacgcttac
     1621 ttactttgct tctcacggac agatggatgg aatgacagtc agcaatgtgt tacataattt
     1681 gttttttgct ttggcaggaa attatattgg tggcggttta gtaatgggac tgggatatgc
     1741 ttggctgaat cagtcaaaat catcctatgt ggactaaaaa gtcgttcatt aaaggggaag
     1801 tgccatggaa gttttagttt ttccaaaaaa tgaggaaaaa gcgttacaaa tggaaaagta
     1861 catgaaaaat cttttttcct ttgctggagt acctaagccg gaacgcgctc ttttacaaca
     1921 accgttactg gatgagagca aaaatttatc tatttctgaa gttttagctt ttgttcatta
     1981 ttattatgaa caaatcgaaa gagagtatca gtatgtagcg atcgatttag tccagaaaaa
     2041 catcaaacgg cttacatttg aagacttagt cgtattgttg ccactagttc agaaaaaaga
     2101 atggtgggac agcatcgact cttggagaaa agtatacagc acatggtgta aagcctaccc
     2161 agagcagcgt gaagaagtct ttcaattgtt tgaaaaaaag gaagattttt ggctacgtcg
     2221 aatagcaatc acgttgcaat taggttataa acagcaaaca gacacaggac tattgatccg
     2281 agcaatagac gaagatcgtc aaacaaagga attctttatc caaaaagcaa ttggttgggc
     2341 attacgcgat tatagtaaga ccgatgccga ttgggtaaaa agacagctga caacagattt
     2401 gagtaagtta gctgttcgag aggcaagtaa gtatttataa gaaagcgaga cgacaagaga
     2461 tgagacgcaa attatttgct tttgatattg atggaacgtt gttagattca aacaaacaag
     2521 ctttagatag cacaagagaa gcattggaaa aattacgcaa agccggtcat ctggtcacga
     2581 ttgctacagg gcggagccga tttcatgcgc aagagatcat tcgcgatctt gattttacga
     2641 actacatttt atgtaatgga gcggccggtt ttttagacca tgaacaagta tataagaatt
     2701 tactagatcg cgaccagttg gatgcgttcg ttcaagaagc gcatcatgag cagatcgata
     2761 cagcttttgt cagcttagac aatatcaagc gttcttcttc caatgatgta acggtgatgg
     2821 aagaagcgat gcattctttc ggtgctgtct tgcctgaatt agatcagttt tttgcagagg
     2881 aacaagaaat ataccaagcg ttagcatttt tcgatcaaac atatgatggg aagttctctt
     2941 catatgataa aattcgattt gtccgctggc atgagaacag tgtagatgtc gtacctaaaa
     3001 atggttctaa agcagcgaca attctgcact tagcagaaca agtaggcatc cctcatgaag
     3061 acatcatcag ctttggcgat ggacaaaacg accgtgaaat gttgcgcatg tcaggaattg
     3121 ggatagctat ggggaatgct gtgccagaag tacaagcaga agctaagatg gtcactgata
     3181 caaacgatca ggatggaatt tggaaggcat tgaaagagtt aagcgtgatt taaaagaggt
     3241 tgtgaaaaag ctgttctgca tcaagtaata agcccagcca aacaaaaata gctcctccta
     3301 tttttcgttt ttctgggctt attaccgcag atgcaagctt tcgaacaccg tttaaggaag
     3361 aggtcgtgac agaagtggtc agtttcaaga aataagaagg gatttacgaa aattgcttct
     3421 caaatttttg taaaattacg gcttatttct gaagaagtag atgaaacaac gtttattcgg
     3481 gaaatgggac tgtgacaaga tgtttgtcat agtcccattt ttttatttta tttttcaaaa
     3541 ctaagaaata ccaggaaaga gttccttttg tttgaccttt tctttcttat tgaaaacaac
     3601 tataatatag agtgatgtta aaagagaaga ggaatttagt gtgcgggtat tgtttattgg
     3661 tgatgtagta ggttcaatgg gacgagagat gattactgaa tatcttccta gattgaagaa
     3721 gaagtaccgt ccgcaagtaa cgattgtaaa tggtgagaat gccgcttctg gacgtggaat
     3781 cacagaaaaa atctataaaa aatttttgca agacggcgta gatgtcgtga cgatggggaa
     3841 tcatacgtgg gacaaccgcg gaatctttga gtttgtaaat gaagctaaaa agatggttcg
     3901 tccggcaaat tttccggaag ggacgcctgg acaagggatg gtctttgtca aagtgaacca
     3961 agtcgaactg gcagtgatca acatgcaggc tcgttctttt atggtggact tggatgatcc
     4021 ttttcgtaaa atgaatgagc tggtagaaga agccagaaaa cgtacgccga tcatttttgt
     4081 agattttcat ggagaaacaa caagtgaaaa acaagcaatg ggctggtttt tagatgggaa
     4141 agtttcagca gtggtcggta cacatacaca cgttcagaca aatgatgcgc gaattttgcc
     4201 aagaggaaca gcgtatctga cagacgttgg gatgacaggt ccctatgatg gtattttggg
     4261 tatgcgcaga gaacctgtaa tcgaaaagtt tctgactgca ttaccaaaac gttttgaagt
     4321 tgtggaacaa ggccgatcga ttttaagcgg ctgtatcttg gaattggatg atacgacagg
     4381 tcatgcaaaa gaaatccagc tgattcagat caacgatgat cgaccgttca tggagtagaa
     4441 agagaggcag ttttttgcag caggagccgg aaattcaaaa agaagtaagg aaactgacaa
     4501 aacttttaag agagaatgaa acgatcattc gctacaaaga acttgaagaa aagatccagc
     4561 aaaaccagta tttagctgag ttgagagaaa aaatcaaaca agcgcaaaaa gatgctgttc
     4621 attttgccca ctatgacaaa ccagcagcag aaaaagaagc aatcaaacaa gcggatcaat
     4681 tcatgcaaga atttgatcag catccgcttg ttgtagctta tagaaaacag ctgttggaag
     4741 cagatgatct gcttcatcat ttaacaacta tgatacaaga agaaatcaat ggacaaatag
     4801 aggaggaaaa acatgcctca aaaaactaaa aatactccta tgatggaaca atatctaagc
     4861 atcaaggccc aataccaaga tgcttttttg ttctatcgtt taggagactt ttatgaacta
     4921 ttttacgaag atgcgatcaa agcgtcacag cttttggaat taacgttgac gagcagaaat
     4981 cgaaatgcag aagaacctat tcctatgtgc ggtgtacctc accatgcagc acaaggatat
     5041 atcgatgcct tgattgaaaa aggctataaa gtagcgattt gcgagcaggt agaagatcca
     5101 aagacgacta aagggatggt caagcgcgaa gtgatccagc tagtcactcc tggtacagtc
     5161 atggacagca aaggattgtc ggcaaaagac aacaattttt taacagctgt agttagtcaa
     5221 ggaaatgaat ttggctttgc ttatgccgat ttaagtacgg gagaattgaa gacggctcgg
     5281 ttgcatgatg aagaagctgt tttaaatgaa gcctctgcat tgcagacaaa agaactcgta
     5341 ttaggcagtg agatcactga tacccttcgt gaacagctaa gcgggcgttt agggctagtc
     5401 ttttctcaac aaaatgagat ggaagaaaac gcggaattta gctttttgac aagcgaacta
     5461 gtcgatccgc tggaaaaaga agcaaccggc aaactattga cttatttatc ggtgacacaa
     5521 aagcggagtt tagctcatat ccaaaaagca gtagaatacc aaccagatca ttttttgaaa
     5581 atggattatt attcaaaatt caacctagaa ttaagccaat ctattcggac tgggaaaaag
     5641 cacggcactc ttctatggtt gttagatgaa acaaaaacag caatgggtgg ccgtttacta
     5701 aaacaatggc tagaccgtcc gcttatccag gcaaaacaaa ttaaagcaag acaagaaatg
     5761 gtcgcttctt tgctagacag ctattttgaa cgtatcgatt tgcagtcagc attaacgaaa
     5821 gtctatgatc tggaacgcct agcaggtcga gtagctttcg gtaatgtcaa tgggcgagac
     5881 ttgatccagc tgaaaacttc tttagaacaa gtgccaatga tcagagggtt gattgaagga
     5941 atcgatcaag gtcaatggca aagtctgctt tcagaaatgc agccgatgga ccatctcgtc
     6001 caattgatcg accaagctat ccaagatgat ccgccgttac aaataacgga aggaaacatc
     6061 atcaaagatg gattcaatga gcagctcgat atgtaccgtt ctgctatgag aaacggaaag
     6121 aaatggttgg cagaacttga agcaaaagag cgtcaagaaa caggaatcaa gaacttaaaa
     6181 atcggattta accgcgtttt cggttattat atagaaatta caaaagcaaa tcttggaaat
     6241 gcagaactgg agaagtatga acgaaaacaa actttagcaa atgcagagcg tttcatcaca
     6301 ccagaactaa aagaattgga gacacaaatc ctcgaagcag aagaaaaatc tgttgattta
     6361 gaatatcagt tgtttttagc tgtgagagaa gaagtcaaaa aggctatcca gccgctacaa
     6421 gtacttgcaa aagcaatcag tgcagtagat gtgctacaaa gttttgcaac gatcagcgaa
     6481 cgctatcagt atgttcggcc agaacttgtc agtgacaagc atcagcttct gatcaaagac
     6541 ggacgacatc ctgtagtaga aaaagtatta ggacatcagg aatatatccc taacagcgta
     6601 gaaatggcag aagacgaaat gatcttgctg attacaggac cgaatatgtc tggtaaaagt
     6661 acgtatatgc gacagttggc attaactgtt ctgatggcgc aaatgggctg tttcgttccg
     6721 gcaaaacaag cagttttacc tatttttgat cggattttta cccgaattgg tgcaagtgac
     6781 gacttaattg ctggacaaag tacttttatg gtagagatga tggaagccaa tcaagccctg
     6841 cgttatgcta cgccaaacag tttgattttg tttgacgaac tagggcgagg aacagcaacc
     6901 tatgacggaa tggcgttggc ccaagcaatc atcgaatata tccatcgtca tgtacaagca
     6961 aaaacactct tttctactca ttatcatgaa ctgacggtac ttgagaaaga acttccgcag
     7021 cttaaaaatg tccatgttgg agccgttgaa aaagacggag aagttgtctt cttgcataaa
     7081 atgatggatg gacctgcgga taaaagctac ggcatacatg tggcaaagat cgcaggattg
     7141 ccttccaatc tactagaacg agcagcagat atcctgcata cgctagaaaa tggcgataac
     7201 ggacaccata ctgttccagc tgaagtagaa gagaaaacta aaacagccaa acaggagcaa
     7261 ataaaaccac ctgcagaaaa agtaaatcaa gaccatatac aagaccgtgt aaaagaagaa
     7321 aaccaacagc tttctttatt cagtgaatta tccgaaaatg aaacagaagt cattgaatca
     7381 ctgaagcaaa taaatttact agaaatgacg ccaatggatg cactaaacac attatatgaa
     7441 ttacaaaaac aactgtagga agaaggcgga tatcgatggc caaaatccaa gaattatctg
     7501 aacgtttagc taatcagatc gctgcgggag aagtagtgga gcgaccagct tccgttgtga
     7561 aagaattagt ggaaaatgca attgacgccg gcagtactca gattgatatc ttagttgaag
     7621 aagcgggact gcggacgatc caagtgattg ataacggaga aggcattttg gcagacgacg
     7681 tagagaatgc cttcaagcgg catgcgacaa gtaagatcca taatcgcgac gatctttttc
     7741 gcatacgaac gctgggcttt cgaggggaag ctttgccaag tattgcctct gtttctgaaa
     7801 tgactctgga aacggcgaat caggaagaaa aacaaggcac ctatctgtcg ctgaaaggcg
     7861 gagaagttat tgagcatcga ccagcaccac tgcgtcaagg aacgaagatt accgtcagca
     7921 atctgttttt caatacgcca gcccgcttga aatacgtgaa aacactgcac acagaattag
     7981 cgaacatcgg ggacatcgtc aatcgactgg cattgagtca tccatcgatt gcttttcgtt
     8041 tagtacatga tggaaacaaa atgctggcaa ctgctggaaa tggcgatctg aaacaaacaa
     8101 ttgcgggaat ctacggatta tctaccgcaa aaaaaatgct gaaaatcgaa ggaaaagatc
     8161 tagattttga gttatttggc tatgtttcat tgcctgaggt gactcgtgcc agtaggaatt
     8221 acttatcgac gatcatcaat ggacgtttta tcaagaactt ttccttgaat aaagcaatcg
     8281 ttgcaggtta tgggtcaaaa ttaatggtcg gtcgtttccc tattgctgta ttggaaatca
     8341 aaatggaccc gcttttagta gatgtaaatg ttcatccgac gaagcaggaa gtccggctgt
     8401 cgaaggaaaa agaactaaca gagctaatta gtaacgccat tcaaaaagca ttgtctcaag
     8461 aaaatctgat tccaagtgca gcggataatc tacggtttaa gaaaaaagta cctgatcagc
     8521 ctaaaacaga acaaatcgaa atcgatttgt cctacgcttt tgaagaagaa aaacctaaaa
     8581 agccaagtag tttgaatttt gatcgagaga caggtacctt tttcgtagaa gaactaaaga
     8641 acacatttgt tgacagaatt gttgataagt ctgtggaaaa tgttgaaaac tattctggtt
     8701 tttctgaaga aaacaccact tatccagaaa aaacagcgaa aacggtggat aagtttggtg
     8761 aaaatgttga taactttgta gaaaaaacag tggaaaaatc tgaaaagaaa gaatttccca
     8821 ttgtggataa catggagaaa acaaatgaag aacaagcaga taaatccgta gaagaggatg
     8881 aaaagattta cgaggaagaa ttgacactcc atcctgaatt tgatttcact caaggaaacg
     8941 acaaaacatt agaacaagcc atcaagaaat tagaaacgga aaaaccagta caacgttttc
     9001 ctcagttgga atattttgga cagatgcacg gaacgtatct ttttgcccaa agtagcgatg
     9061 ggttgtacat cattgatcag catgctgccc aagagcgcat taagtatgaa tatttccggg
     9121 aaaaaattgg ggaagtatct gatgatctgc aagaattgct tgttccattt gtgttagact
     9181 atccaaacag tgatgctttg aaattaaaag agcaaaaaca cttgctagaa gatgcaggga
     9241 tttatttaga agaatttggt caaaacagct ttattgtacg ggcccatcct acttggtatc
     9301 ccaatggaga agaagaatcg attattcggg agatgatcga tatgctgctg acaactggaa
     9361 gtgtgagtgt aaaaaaattc cgtgaagcaa cagcaatcat gatgagttgc aaacgatcga
     9421 ttaaagccaa ccattatttg aatgaagcac aagcacgtgt gctgctgaag gatctggcac
     9481 gatgcgaaaa tcctttcaat tgtccccatg gccgtccagt attgattcac tttaccaatt
     9541 cggatatgga aaggatgttc aaacgaatcc aagacccgca ttaagtcaaa gagaagaggg
     9601 aaaacatgaa gattgtatta gcatcccaat caccaagaag aaaagagtta ttggctcata
     9661 tcgtttcaga attcgagatt gaacctgctg atatcgatga aacgccattt ccagacgaac
     9721 agcccgtcga ttatgttcgt agaatggctg aagaaaaagc acgaactgtc tgggaacaag
     9781 atgaaaaaga agaccattta gtgattgcca gtgacacgac tgttgttctc aatcaagaga
     9841 tcatgggtaa accagaagat ctggctgagg ctgaattcat gctgaaaaaa ctaagcgggg
     9901 aaacacatac tgtatataca gctgttgttt taaaaacaaa ggaacgagaa gaacggatac
     9961 tagcagaagc acatgtcact ttttatcctt tgacagacga agagatcaaa cgctacttgg
    10021 agacggggga ttatgcggat aaggcagggg catacggcat ccaaaatgct gcgagtgtat
    10081 tcgtcaaaga aatttctgga gattattaca gtatcgtagg atttccgatt ggtgctgtca
    10141 atcaggcact gaaaacattc atttaaacaa aaaagcggac tatctgattt tcgattgaaa
    10201 atccatgata gcccgttttt tttattgaaa agattactta tctactctga ctaagatttt
    10261 acttcagctt taccgactgc ttcgcccggg cgaacaagtc ctgattggtc aagagaaagt
    10321 cctgcaactt tttcattatt ggtcaaagca acaataatgt cagttttctt gccagcttgt
    10381 tctatttctg gcaaatccat ttcagcaatc agggtctctg gtgtgacaag gtatccttct
    10441 ttcacgaata cattgaaagg aacacccttt agttcaacag tatctaatcc catgtgaaca
    10501 agtacttcca gcccctcatt agaaagaata ccaatggcat gttttgtttc aaagacactc
    10561 gtcactttac cgtttactgg tgcatagact ttgccgtttg acggttcgac agcataaccg
    10621 tcacccatca ttttttgtga gaaaactggg tcatttactt cagttatttc cttgatggtc
    10681 cctgcagcga ctgcgaataa tgtttctttt gttgcaactg ccgtaccagt tgctgaaaca
    10741 ggttcgaaag actgtggcgt tgctgttgcg ccatttgctg tttgaagttg tacatcagca
    10801 gcattttcag gaacagcagg atcaatctta ttcaaaattc cctgacgtct aaagatgatc
    10861 gtcaagccaa atggaatgat gacagcaatg atcattgcga tgatgaaagg tacccatgtt
    10921 tctgcttgga tagctagtat tcctggcaaa ccgcctactc cgatcgcgtt agctcgaaca
    10981 cccatcaagt tagcaaacat gccagctaat ccagagccga ccattgcagc gacgaaagga
    11041 tagacgtatt tcaaattgat tccgaacata gcaggttcag ttacgcctaa gtaacaagaa
    11101 atcattgctg gaatcgagat ttgttcttct tttttattcc cacgatgtaa gaagatgatg
    11161 gctaagactg cagagccttg ggcgatattt gataaagcaa tcatcggcca taaattcgtt
    11221 gatccgaaat ctgcaatcag ctgcatatcg atggcattgg tcatatggtg gagaccagta
    11281 atcactaaag gagcatacag gaatccgaat acggcactaa acaaccagct gatagaagac
    11341 gtcaatccag tatttacgat atttgagatc cagcttccga ctgtccagcc gataggacct
    11401 aaaataacgt gtgcagctaa aacagttggt aacaatgaaa acaatggaac aaaaatcatc
    11461 gagatcgact gagggatata tttgcggaag aagatttcta gatacgctaa cataaaccct
    11521 gccagcatgg ctggaataac ttgcgcttga taaccaatca tctggacttg agcaaaacca
    11581 aaatcccaaa atggaatatc tgctgcggct gtggaagcta cactataagc atttaataat
    11641 tgcggagaaa ctaacgtaat tcctaaaacg attcctaata tttgtgtagt acccatcttt
    11701 tttgcaatac tccaagtgat acctactgga agaaaatgga aaattgcttc acccggtaac
    11761 cacaaaaagg cattgacacc attccaaaac ttcgagtgtt caacgattgt tccaccaaga
    11821 ctttcaaact ggataccttc aagtacattt ctgaatccaa ggatcaatcc accgacaatg
    11881 attgcaggaa tcagtggtgt aaagatttct gctaaaacgg caatagcacg ttgaacggga
    11941 tgaaggtttt gttttgcagc tgcttttcct tgttctttgg aaaccccttc aactccagat
    12001 actgcaacga agtcgttata aaaagtagaa acatcgtttc ctatgatgac ttggaactgt
    12061 cctgcattcg tgaacatccc ttttacgcta ggtatatctt cgatcgcttt ttcgtccgct
    12121 tttcctggat cattcaggac aaaacgcata cgagtcgcac aatgactaac tgcagcaata
    12181 ttttcttttc cgccgatatc atgtaacagc ttctcggcat ctgcttgata ttttgccata
    12241 tcaataaacc cctttctctt aacttgtcta tgcaagttgt ttcttcattt actatacttt
    12301 ttttcggaga gaattgcaag cgttttatta aaaagtacga gagaaaatga tatcgtttta
    12361 aaaagcttaa aataagcttt tcttaaaaat aggaacattg aaatacaaaa agagctgtag
    12421 tatagtgaag aaatgatgga agaacgagtg agggaaaaaa catgaataag ttcaatgaaa
    12481 tttttttaga tttggaacag aaaattttag cgaaagaata tcctccgcat tcgcttttac
    12541 ccagtgagaa ccaattgatc aaaatttatg gtgtttcacg agaaacaatt agaaaagcgc
    12601 tgaatctttt gactacggaa ggttatattc aaaaaaaaca aggaaaaggt tcgatcgtac
    12661 tggatcggaa tcgcttcgac tttcctattt ctggattgac tagctataaa gagctacagg
    12721 agacccaacg tattccaagt gaaacgatca tccatacgtt agaagaaaca gaagtgacaa
    12781 aagcattgaa cgagatcact ggctgggaga tcggtgcgcc tgtttggcat ttgatacgcg
    12841 aaagaaaaat caatggcgaa gtagttatat tagacaccga ttatctatta aaggaaatcg
    12901 ttcctatttt aactccagca caagcacaag gatcgatcta tgaatatttt gaaaatgagc
    12961 tttctttgac gattgcttac gcgcaaaagg agatcacagt agaagaagta acggaaatca
    13021 tgaaaacagc gatggatctt cacgatgaca cttatgcagt agtagtcaga agcctcgttc
    13081 atctggaaga tacccgctgt ttcgagtatt ctgagtcggt tcaccgatta gataaattcc
    13141 gtttcgttga gtttgcccga cgccgaaaag tgtaaatagt tcaaaagaaa gcaaattgtt
    13201 ggcatctgtc cactttggcg ctatgatagg atcacaaagg gggcattttt aatggataaa
    13261 ccaataagac cagaaaaaga agaactgaaa gaaaagttat cacccatggc ttatgcagtc
    13321 acacaagaaa atgcaacaga acgaccattt agcgggaaat atgatgattt ttacgaaaag
    13381 gggatctatg tagatatcgt cagcggtgaa ccgttattca gctctgcaga aaaatatgat
    13441 gcaggttgtg gctggccatc attcagcaaa ccaataacga aaagacaagt gcgcgaaaaa
    13501 gccgattttt ctcatggcat gcatcgaata gaagttcgaa gcaaagaagc agattctcat
    13561 ctcggacatg tgtttaccga tggaccagta gatcaaggtg gtctacgtta ctgcatcaat
    13621 tctgcctcac tgaaattcat tccatatgaa aagatggatg aattaggtta tggggaattc
    13681 aaatcacttg tagaataatg gaaatacaca aagagcggta caatacgggg atttactcga
    13741 tcgtccgctc tttttttatt caagcgattg ctatttgaaa gaaacgctcc ctagactcct
    13801 tttttcacaa ggtgaaatgt gctacaataa agggaacgta tgtgtgcaaa agaaagagga
    13861 ctagaatatg tatgaatatc tgacaggtgt ggttaccttt atcaacccct attacatagt
    13921 agtagaaacc aatggtatcg gctaccagat cgcactagga aatccttacc gctacagtag
    13981 caaactaaat caaaaaataa aaatttatgt ccatcaggta atacgtgaag atgcgcatct
    14041 tctgtatgga tttgattcgc tagaagaaaa gcaactgttt ttgcgattag taagtgtttc
    14101 aggaatcggt ccgaaaagtg cccttgcaat catggcaagc gaagaccact ccggtttgat
    14161 tcaagcagta gaatccagtg atgtcactta tttgacgaaa tttccaggag tagggaaaaa
    14221 gacagcacaa caaatgatct tggatctaaa aggaaaattc ggtgagctga gtatcgatac
    14281 accttttagt ctatttgatg aagcaaacac aaaagacgct acagctctat ctgaagcaat
    14341 agaagctctc tcggctcttg gttacagtga tcgagaaatc aaacgtgtag aaaaacaatt
    14401 aaaagaagta gataatcaga caacggatga gtatctacgc caagcactca agctaatgat
    14461 gaaaaaataa ggaggagacc aaatgacaga tgaatacttg ttatcaccag aatctggcga
    14521 aaatgaactg agtttggaaa aatctcttcg accacagctg ctttctcaat atatcggaca
    14581 gagaaaagtc aaacaagaac ttcagattta catcgaagct gccagaaatc gggaagaacc
    14641 tttagatcat gttctgttgt acgggcctcc tggattagga aaaacgacga tggccatggt
    14701 cattgctaat gagatgaccg taaatattcg tacgacgagt ggccctgcta tcgaacgtcc
    14761 gggtgactta gtagcaatct tgaatgaact ggaagcaggg gatgtcctgt ttatcgatga
    14821 gatccatcga ttacctagag tggtagaaga gatgctttat tcagctatgg aagatttcta
    14881 tgtagatatc atggtaggac aagggccaac cgcacatccg gttcatttcc ctcttcctcc
    14941 ctttactttg attggcgcga cgacacgggc aggtatgctt tctgcacctt tacgagatcg
    15001 atttggcatc atttctcata tggaatatta tgacgaacag gatctgcagg agatcgtttt
    15061 acgttcttcg gatatttttc aaacagagat cattgaagaa ggggcattgg aaattgctcg
    15121 ccgatcacga ggaacgccta gaatcgctaa tcgcttgctc aaacgggttc gtgattttgc
    15181 gcaagtccaa ggagacggga aaatcgatcg tcctatagcg gatcaggcac ttactttgct
    15241 acaggtcgat caggctggat tggattatgt tgaccagaaa ttgttgcgaa caatgatcga
    15301 gttatatggc ggcggaccag tagggttaag tacgctttca gtaaacatag gagaagagcg
    15361 tgagacggta gaagatatgt atgaaccgta tctgatccaa aaaggatttt taaagcgtac
    15421 acctagaggg cgaattgcta ctgcttatgc ctatgaacat tttggttacg actatcatat
    15481 atgatttttg cttttagatt ggaggaaaat gaatgagtga tacaacacgt acgaaacaat
    15541 tgatcaaaaa cgtattgatt gatttgtgcc aaacgcaatc ttttcgaaaa atcagcgtcc
    15601 aaaaaatcgc aaacgaagca aaaatcaatc gacaaacatt ttattatcat tttacagata
    15661 aatacgattt gcttcgttgg gtctattgtg aggatgcatt gcattattta gatccacaat
    15721 gtctttcact tgataattgg gaagagcaag cattgaaaat gctgaaagca attgctgaga
    15781 agaaagaatt ttatgcaaat actgtgcttt ctgacccaga aattttacaa aaagagtttt
    15841 ctcaactgat ccaaaaatca tttatcaaga tttttgaaca aatggatgaa gaaagacaac
    15901 tttctgcaag agacaaacta ttttatgctc gttttttttc ttacggttgc agtggtgtat
    15961 tagtcaattg gatcacgaat gaatatgaag aaacaccgat ggaaatcgct atgcagcttt
    16021 tccgtttagc caaggatacg gaattatatt cttatcgtct ttatgcaaaa gaagagttgg
    16081 aagaacagta atagaaaaat tttttgttgg aaaagcaata atcgctaaaa ggagatgggg
    16141 aaatgacaaa aattttattt gtttgtttgg gaaatatttg ccgttcaccg atggcagaag
    16201 gcttgctgag aaaacgaata gcattagaag gaagggaagc agaatttttt gtggattctg
    16261 ctgctacaag cacgtatgaa gttggaaaga caccgcaccc tggaacgaaa aaaatattaa
    16321 accaagaaaa agtagatatg acgaatatgg ttgctcggca aatcacaccc cacgattttg
    16381 aaacttttga ttggatcatt ggcatggatc aagaaaatgt agaagaactg aaacgaagag
    16441 caccacgctc agcgcagggg aaaatccatt tgtttttaag tagcattcct ggaaaagaaa
    16501 aagaaaatgt tcccgaccca tactatacca ataactttga tgagacatat cacttgatca
    16561 cagaaggcct gaatcattgg tggaatattt ggattgatga aaataaaata acaaataatt
    16621 tgtaaagtta acgctttctt tttaaggcga tgttagagca tttccttgtt tttttgtaaa
    16681 atgttaggta aaataaaatt aattaagtaa cactttctta gctaaaggag gagtgcattc
    16741 gatgaacgaa gagacagtat cgccaatgaa gaagcttacg aagttagtta ctcatacttc
    16801 gtatcaactt tttgattctt ttgtaaatga cactgaagaa catgctaaga atgtacgatt
    16861 gcaaggaaag tttattcttg agctgcaaaa agctgctact gaacatagtc tagttatctt
    16921 acaagtaaga gcaaatcgtt tgagtagcaa gtatgaaacg atctcaggct gggtggcgac
    16981 aaaaacggtc gaaaaagacc gagtgatgat ccgcctacaa aacgaagagc agcagatccg
    17041 gatggttcct gtagaacaaa ttgaaaaaat aacaatcctt tcaccagcag gggaacagga
    17101 acggttatca aaataattta taccagatag aaaagcacgt atgagcttgt accatcaatc
    17161 attactaatc aaaaataacc gaatgcttta agctgatatc agtaaaacgg aacgttttag
    17221 gatgagtatc atagcatgac agcattcggt tgattagttg aatcgattgg atggtacaag
    17281 tttttttgat aaaaaaaggt cctgctagcc ggggagctag caggataagg agttaaaaat
    17341 gaaaaagtgt tgttagggtt gtttgttggt atgcttatat aatagtcatt agttgtgaat
    17401 attttgtgac gaaagttttc gaaagttgtt aagtttt
//
LOCUS       DAZULY010000034        17141 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000034 DAZULY010000000
VERSION     DAZULY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.034"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..369
                     /locus_tag="ACG81A_000766"
     CDS             1..369
                     /locus_tag="ACG81A_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFS5014157.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="ACG81A_000767"
     CDS             549..1490
                     /locus_tag="ACG81A_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFS5014158.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="ACG81A_000768"
     CDS             1559..2290
                     /locus_tag="ACG81A_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5014159.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="ACG81A_000769"
     CDS             2434..4614
                     /locus_tag="ACG81A_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFS5014160.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="ACG81A_000770"
     CDS             4634..5116
                     /locus_tag="ACG81A_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFS5014161.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="ACG81A_000771"
     CDS             5132..5893
                     /locus_tag="ACG81A_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFS5014162.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /gene="murC"
                     /locus_tag="ACG81A_000772"
     CDS             6024..7358
                     /gene="murC"
                     /locus_tag="ACG81A_000772"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFS5014163.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7665..8150
                     /locus_tag="ACG81A_000773"
     CDS             7665..8150
                     /locus_tag="ACG81A_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFS5014164.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8143..8826
                     /locus_tag="ACG81A_000774"
     CDS             8143..8826
                     /locus_tag="ACG81A_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFS5014165.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8952..11597
                     /gene="polA"
                     /locus_tag="ACG81A_000775"
     CDS             8952..11597
                     /gene="polA"
                     /locus_tag="ACG81A_000775"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFS5014166.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11642..12478
                     /gene="mutM"
                     /locus_tag="ACG81A_000776"
     CDS             11642..12478
                     /gene="mutM"
                     /locus_tag="ACG81A_000776"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFS5014167.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12442..13071
                     /gene="coaE"
                     /locus_tag="ACG81A_000777"
     CDS             12442..13071
                     /gene="coaE"
                     /locus_tag="ACG81A_000777"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFS5014168.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13123..13455
                     /locus_tag="ACG81A_000778"
     CDS             13123..13455
                     /locus_tag="ACG81A_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS5014169.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13767..14258
                     /gene="nrdR"
                     /locus_tag="ACG81A_000779"
     CDS             13767..14258
                     /gene="nrdR"
                     /locus_tag="ACG81A_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFS5014170.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14281..15654
                     /locus_tag="ACG81A_000780"
     CDS             14281..15654
                     /locus_tag="ACG81A_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS5014171.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15654..16580
                     /gene="dnaI"
                     /locus_tag="ACG81A_000781"
     CDS             15654..16580
                     /gene="dnaI"
                     /locus_tag="ACG81A_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFS5014172.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16709..>17141
                     /locus_tag="ACG81A_000782"
     CDS             16709..>17141
                     /locus_tag="ACG81A_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014173.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSL"
BASE COUNT         5837 a         3003 c         3782 g         4519 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7561 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7621 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7681 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7741 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7801 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7861 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     7921 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     7981 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8041 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8101 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8161 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8221 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8281 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8341 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8401 tactctcttt tgaatggcat aatattagcg gttactcttg catactacga tattggaaca
     8461 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8521 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8581 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8641 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8701 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8761 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8821 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     8881 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     8941 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9001 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9061 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9121 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9181 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9241 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9301 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9361 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9421 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9481 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9541 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9601 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9661 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9721 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9781 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9841 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
     9901 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
     9961 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10021 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10081 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10141 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10201 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10261 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10321 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag
    10381 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10441 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10501 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10561 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10621 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10681 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10741 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10801 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10861 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    10921 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    10981 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11041 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11101 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11161 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11221 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11281 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11341 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11401 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11461 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11521 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11581 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11641 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11701 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11761 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11821 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    11881 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    11941 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12001 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12061 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12121 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12181 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12241 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12301 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12361 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12421 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12481 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12541 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12601 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12661 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12721 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12781 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12841 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    12901 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    12961 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13021 atacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13081 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13141 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13201 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13261 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13321 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13381 gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13441 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13501 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13561 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13621 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13681 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13741 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13801 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13861 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    13921 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    13981 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14041 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14101 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14161 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14221 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14281 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14341 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14401 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14461 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14521 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14581 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14641 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14701 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14761 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14821 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    14881 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    14941 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15001 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15061 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15121 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15181 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15241 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15301 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15361 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15421 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15481 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15541 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15601 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15661 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15721 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15781 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15841 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    15901 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    15961 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16021 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16081 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16141 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16201 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16261 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16321 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16381 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16441 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16501 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16561 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16621 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16681 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16741 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16801 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16861 atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa cgcttcagtt
    16921 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    16981 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17041 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17101 accgcaagtt cctggagatt cgattattga tgtttcattg a
//
LOCUS       DAZULY010000035        17109 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000035 DAZULY010000000
VERSION     DAZULY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.035"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..145
                     /locus_tag="ACG81A_000783"
     CDS             <1..145
                     /locus_tag="ACG81A_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5014174.1"
                     /translation="KNKAYNLLKDRSIENIKIGARYKIPKKAVIEYILKIQDEGKNHA
                     IIK"
     gene            189..1358
                     /locus_tag="ACG81A_000784"
     CDS             189..1358
                     /locus_tag="ACG81A_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5014175.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            1932..2501
                     /locus_tag="ACG81A_000785"
     CDS             1932..2501
                     /locus_tag="ACG81A_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFS5014176.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            2488..3348
                     /locus_tag="ACG81A_000786"
     CDS             2488..3348
                     /locus_tag="ACG81A_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5014177.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            3538..4242
                     /gene="yycF"
                     /locus_tag="ACG81A_000787"
     CDS             3538..4242
                     /gene="yycF"
                     /locus_tag="ACG81A_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFS5014178.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            4247..6082
                     /gene="walK"
                     /locus_tag="ACG81A_000788"
     CDS             4247..6082
                     /gene="walK"
                     /locus_tag="ACG81A_000788"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFS5014179.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            6079..7395
                     /gene="yycH"
                     /locus_tag="ACG81A_000789"
     CDS             6079..7395
                     /gene="yycH"
                     /locus_tag="ACG81A_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFS5014180.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            7396..8265
                     /locus_tag="ACG81A_000790"
     CDS             7396..8265
                     /locus_tag="ACG81A_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFS5014181.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            8320..9129
                     /locus_tag="ACG81A_000791"
     CDS             8320..9129
                     /locus_tag="ACG81A_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5014182.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(9232..9861)
                     /locus_tag="ACG81A_000792"
     CDS             complement(9232..9861)
                     /locus_tag="ACG81A_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5014183.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            10031..11323
                     /locus_tag="ACG81A_000793"
     CDS             10031..11323
                     /locus_tag="ACG81A_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5014184.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(11399..12061)
                     /locus_tag="ACG81A_000794"
     CDS             complement(11399..12061)
                     /locus_tag="ACG81A_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS5014185.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            12286..12570
                     /gene="groES"
                     /locus_tag="ACG81A_000795"
     CDS             12286..12570
                     /gene="groES"
                     /locus_tag="ACG81A_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFS5014186.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12621..14246
                     /gene="groL"
                     /locus_tag="ACG81A_000796"
     CDS             12621..14246
                     /gene="groL"
                     /locus_tag="ACG81A_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFS5014187.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(14335..15474)
                     /locus_tag="ACG81A_000797"
     CDS             complement(14335..15474)
                     /locus_tag="ACG81A_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5014188.1"
                     /translation="MWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWKKAQ
                     KILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHISDD
                     TIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPALAVK
                     IHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRYGEL
                     LALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIESVIA
                     DNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSHVSL
                     LSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(15585..16169)
                     /locus_tag="ACG81A_000798"
     CDS             complement(15585..16169)
                     /locus_tag="ACG81A_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFS5014189.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(16296..16727)
                     /locus_tag="ACG81A_000799"
     CDS             complement(16296..16727)
                     /locus_tag="ACG81A_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS5014190.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(16745..17065)
                     /locus_tag="ACG81A_000800"
     CDS             complement(16745..17065)
                     /locus_tag="ACG81A_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5014191.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
BASE COUNT         5670 a         2883 c         3456 g         5100 t
ORIGIN      
        1 aaaaaataaa gcttataatt tgctgaaaga tagatcaata gaaaatataa aaattggagc
       61 aaggtacaaa atacctaaaa aagctgtcat tgagtatata ttgaaaatac aagatgaagg
      121 taaaaatcat gctataataa aatagtcaaa atttttgccg atatttcagt ttaagaggag
      181 agattgaatt gaaattaaga gcaaacataa ttgaaaaaag aggaacatta caagtagttg
      241 tatcttataa agactctaac ggaaaaatta gacaaaagtg gaaagataca aaattaaaaa
      301 agaaaggaaa taaaaaacga gcagaaaaaa ttagagatga atttttacta gaattggaaa
      361 gtcaattagc tatggaatgt gaatgtgcta atccaaattt attgttttat gactatttgc
      421 ttaattattt ggaagtatca aaaaaacaag tagcatttaa cacatatgtt gggtataagc
      481 attacgtcca taatcgaatt tacaagtttt tttatcctaa aaaaattaaa ctaaataggt
      541 taaagcctta tcatttacaa gagttttatc aatatatgtt aaatgataac tgtacagcaa
      601 atacggttat tcattatcac gcatttatac gcaaagctct tcaagaggct gttattacag
      661 aattgataag tacaaatgta gcagataaag taagaaagcc aaaaaagaaa caatttgttt
      721 ctcaagttta taatcaacaa gagatcttga aattacttga cattttgtca gaagaaaaac
      781 tctatttggt agttctttta acagcatttt atggattgcg aagaagtgaa gttttaggat
      841 taaaatggtc tgctattgat tttttaaaca aaaaaatcat gataaaccat gtaatcatag
      901 aaaatccaga gaatatgtca cagttaataa agaaagatca aacaaagaac tcttccagct
      961 atcgtacatt acctttggtt gaaacgattg aacgagcatt agtaaatcaa gctaaatggc
     1021 aacaagataa tcgtattctt ttaggagatg attatatttt agaagataat gagtacgtat
     1081 ttactatgga agatggacgg ttgatgaagc ctcaatatgt aacgcatcgt ttgagtaagt
     1141 tgattaagaa aaatggattg aaaaaaattc gctttcatga cttgcgtcat tctaatgctt
     1201 caataatgtt agatagtggc cagaatatga aatccattca agaatggctg ggacatgcat
     1261 cgtattcgac aactgctaat ttatacacgc atttaaccgc aggagtaaaa gatcgtgcag
     1321 cagaagcatt agaaaacgca tttgggttta aagaataaaa aaacaaaaaa caatatcttt
     1381 ggggtatcgc ttcttgtttt tagaattttt ttgttggcaa tttgttggca gtatgttggc
     1441 aatgaaaaaa ttcacaaact aaaaacctat ttataaagct ttataaaact tttttcacaa
     1501 atagagcatt tggttaagag tcagctgctc taccaactga gctaacggcc cgtacaaaaa
     1561 gtacttaata atagtagcac taagaatttt aaaatgcaag actacattta aagaaaatca
     1621 gaaaaatctt tacttcctta taggaaatca ttcatagatt attcacattt gctcgctatt
     1681 atataagcat accaacaaac aaagttcttg caagagaaca aaccctaaca cttttttcat
     1741 tttttactcc tttcccgccg tctccccggc gggtctcctg ttcttaacga taaaagacaa
     1801 gaaggcttag ccttcttgtt ttttgttttc atggaatccc gctaatcaaa tcacctagtt
     1861 aaagcaacta gattgaatgt ttatcataag tctgatatca tgtttatgaa atgaaaaaag
     1921 gagcggttat gatggaaaaa atgcgtcagc aattaatgga gtgggggaag gaactagaaa
     1981 cattccgtct gcctcactgg gaagaactcc ctgacttaga actttatatg gaccaagtca
     2041 gaacgttagt tgatcgttat ctttcacctg tgatacaagg agaaaagcat ccattgctta
     2101 cctcttctat ggtaaacaat tacgttaaac taggtttgat accagcacca gttaaaaaaa
     2161 gatataacaa agaacacgta gcatttttac ttgcaatcac tacgttgaaa caagtattga
     2221 ctattcctga aatcaaagaa gggatattgt tccaaggaaa aacagtagga attcgtgaag
     2281 cctataatct attttgcgat gaacaagaag cagctgtgtg gatggtcagc caattagcac
     2341 aaggtaaatc tcatccgcaa aaattcgacc aggcaacccc tgtagaatat attgctgtaa
     2401 gagcggcaac tttatcgttt gcaatgaaac ttcttgcgga aaaaacgatt gttctagaaa
     2461 ccgaatattt aaaagaggag aaaacaaatg aaaaacaata aaatcgcaat tttagtagat
     2521 tcaggtacgg atgttccaga agaattgata aaaaaatacg agatgtatat gatcccattg
     2581 aaaattattt ataaagatca ggtatataca gataaaattg acattacccc tgaagagatc
     2641 tatcaacgtt tgccagagga aatccctggt acatctttgc cggatggtga gtcgatcaca
     2701 gaaatctttg agcaaatcaa aaaagacggt tatgaaaagg tacttgcggt aacaatttcc
     2761 agtggattga gcgggacata taatgtggtt cgtttaattg cacaacagca agaagaattg
     2821 gaagcatatg tactagatac gaaaaatatt ggtattggtg caggtttttc tgcgatccaa
     2881 gcagcaaaat ggctggaaga aggagtagaa tgggatcaac tgatcagtaa tctgaatgag
     2941 ttagtcgaaa gaacaaaagt tttctttaat gttgcgacat tggaatatct tcaaaaaggt
     3001 ggacggatcg gtttagttgc ttctattgta ggaaccgctt taaagcttaa cccaatcatc
     3061 tcatgtaatg aagaagggat ttattacaca gtaggaaaag ctaggggacg taaaaaaagt
     3121 ttggataaaa cggttgctta cgtcaaagaa aaaatcggta atgcaaaggt cttcaactta
     3181 gctgttgccc atggggatgc gaaagaagaa gcaattgaaa tggaagcgag acttaaaaga
     3241 gagttcccac aagcaaacca aatctacttt ggccagattt ctccggcatt agtagtccac
     3301 actggtccgg gattattggg tgtcggtgta caagttttag aaggataaat caaaaaaagg
     3361 aaacactgag gtgaaaatca gtgttttcct tttttattga agttagaaca aaaaataata
     3421 aagtattttg cactttcatt ttaattttct cttttgtttt ataggcaaag atgaaacaaa
     3481 agcagaaata cggtataatg agaaggtatg acaataatat ataagaggtg gctctttatg
     3541 aaaaaagttt tagttgtcga tgatgagaag ccgatttcgg atatcgtcaa attcaattta
     3601 gccaaagaag gctatgacgt ttatactgca tatgatgggg aagaagcctt ggaaaaagtt
     3661 gctgaggtag aacctgattt gattttactt gatttgatgc tacctaaaat ggacggctta
     3721 gaagttgctc gtgaggtacg caaaacttat gatatgccga tcattatggt aacagccaaa
     3781 gattctgaaa ttgataaagt cctaggctta gagttaggcg ccgatgatta cgtgaccaag
     3841 ccattttcca accgagaatt agttgcacgt gtcaaagcaa atcttcgtcg cggtgccacg
     3901 gctgcgaagg aaccagaaga ggcagcacct gcggaattga cgattggtga tttgacgatc
     3961 catccagaag cttacatggt gacgaaacga ggagaaacaa tcgagttaac acatcgtgaa
     4021 ttcgaactac tcttttactt ggcgaagcac cttggacaag tgatgacaag agaacattta
     4081 ttacaaacgg tttggggtta tgattatttt ggagatgtac gaacagtgga tgtaactgta
     4141 cgtcgtttgc gtgaaaagat cgaagataat ccaagccatc caaattattt agtgacacgt
     4201 cgtggtgtcg gttactattt gcgtaaccca gaacaggagt aattggatga aaagaaaagt
     4261 ccgctttttt cgatcagtca attttaagat cgctattacc tttattctga ttttattgat
     4321 ctcgatcgaa attattggtg cctactttat acgtggttta gaacgttcca cgatcaatac
     4381 atttattaaa gatatgaatc aaacggtgga atcattagca acaacaatca gcccggaatt
     4441 gaatcgaaaa gataatgcag atgatgaaga ggtaaatgcg aatatcaaaa gatttattga
     4501 aaacagtgcg actagcgata tcatcgaaat acgggtcgtt gacgaaaaag ggatcatccg
     4561 aggaacaacc gatgtcaatg aacaaagcgc tgtgggaaag aaaaatgatt atgttgatat
     4621 caatgatttc acgactaaac gttatgttgc tttagataac gacgataaac gggtgaatat
     4681 caatgttcaa ccgatcctgt ccccgaccgg cgatgccgtt atcggggctc tttacgtcaa
     4741 aagtaatatc gaacaaaaat attcggaaat caataacaca gcagtcattt tctttactgc
     4801 ctcactaatt gcggcattta tttcaatgat cgtatctgtt ttagttgctc gttcgattac
     4861 acagccgatc ggtgaaatga gagaacaagc cattcgaatc gctagaggag actacagtcg
     4921 gaaagtcaaa gtgcatggac aagatgaact tggacagctg gctgatacct tcaatcagtt
     4981 agctgaacgt attgaagaag ctcaagatac aatggaagct gaacgcaatc gtttggatag
     5041 cgtgctatcc catatgacag acggcgtcat tgcgacagat cgcaggggta aagtcattac
     5101 gatcaatgat atggctgttt ccttgctaga tatcaaaaac gaggaagcaa tcggacaatc
     5161 gattttgaca cttttagata tcgaagaaga atatacgttg cggaaattat tagaagatcc
     5221 aaacgaaatg gtacttgacc gttcaaccag tacattggaa agtgatcaga tgatcttgcg
     5281 agtagacttt tctatgatcc gtcgcgaatc aggttttatc agcggattag tagctgtctt
     5341 gcatgatgtg acagaacaag aaaaaacaga acgggaacgt cgtgaattcg tctctaatgt
     5401 ttcccatgaa cttcgtacac cgttgacaag tatgcgtagt tatatcgaag cattgagcga
     5461 aggtgcgtgg aaagacgagg aagtagcacc aaattttttg aaggtaacgc tagatgaaac
     5521 agaccgaatg attcgaatga tcaatgacct cttaaatcta tctcgcatgg atacaggtaa
     5581 tacccagctt caattagaat atgtcaattt caacgagtta gtgaattttg ttcttgaccg
     5641 gtttgatatg atggtgggaa atcaggaaaa aaattataag atccgtcgtg aatttacgca
     5701 aagagatctt tgggtcgaag tagatacaga taaaatcatc caagtagtcg ataatatcat
     5761 gaataacgcg atcaagtatt caccagatgg gggaacgatt acttgtcgct tattagaaac
     5821 gcataataac gtgattttaa gtatcaccga tcaaggattg gggattccta aaaaagatct
     5881 gaaccgagtg ttcgagcggt tctatcgagt agataaagca cgtgcaagag cacaaggtgg
     5941 gacaggactt ggattagcta tttctagaga agtgattaag gcgcatcgag gcgctatttg
     6001 ggcagagagc aaggaaggaa aagggtcgac tttttatatc tcgctgccat atgaacctta
     6061 tgaggaggaa tggtgggaat gagaatctct gaaaaagtcg ttcgtatagg actcattttg
     6121 atggttgcgt taagcatata cttctcttat gcaatctggc tgagcccagc aggaaaaagt
     6181 tcaatcaacg tagatgagac aaacactcaa atgatcgagt ctcaaagcaa tcgaaaaact
     6241 tccgaagtct ttcttccgct tcatgttaca tggcaccatt cgggaatgat ccaagaaacc
     6301 aacagtgaaa atctcgtgta ccgattgcaa ttgatcatcg ataaagcaag atttggaaag
     6361 ctttctgaag tagtttcagg agacaaatca cagtttgaac aggtgaaaaa tctgaactct
     6421 ggttttgagt tggcctataa tgcgcctttt ttgctaagag aatacaagga agcatttgat
     6481 ttagatttag atgtgtcgaa cttggcaaaa gaaagcgaaa atatgtattt tactagaata
     6541 caattcgatc aaggaaataa aaaaatccga tttttgaatt attcgaacca tctcgtatat
     6601 gaagcaaata tttcgttgaa ttggacagat atcaataaag aattaacagc aaaagacaca
     6661 aaatggacag aaatgacaac agaaccatcg acgatcgaca cgcaatatgc tacgaaacat
     6721 tcgatcaaac tgaagaagta tagttatatc ctgtcacagc aaccctatac actttttaga
     6781 aatgcttttt tccagaaacc agatagcgtg aaaaataatg atgaaagcaa tgaattgatt
     6841 ttttacggtg ggaatgaaac aatgtctatc cattcggaaa cgcaagtagt tgattttcgt
     6901 ggagagctgc cagagaaaaa agaaaatgct agtgtatttt ctgaaagttt cccctatatt
     6961 agcaaattag gcagttcttt agggaacctc agatattttg accgcagtga caatacgatc
     7021 gattatcgga tattcgtgga aggcttcccg gtttttggga cagacagcag aggacagatt
     7081 ggtgtggaag ttggcggtga aacgaatggt tctgtgcaag tgaatatcca aaccagtttg
     7141 aatacgattc aagtgccgat accttcagac gaagaagtag aacttgcaag tacgaatgaa
     7201 atcatccagc agttaaaagc taagggagca gatagcaaca agattggttc agtgatcgtt
     7261 ggctatacat ggcaaaatgt caaagaaacg aatcgtgtcg ttgatctctt acctgaatgg
     7321 tatgtgaaat ataaagataa ctggtattca gctaacgacc ttctggaaaa attacctgga
     7381 ttggaggcga attaattgga tttcaaaaaa atcgaatgga tatttttcgt cgctttttta
     7441 gggttgaatg tgtttttatt cagcagttat catgacgcgg tataccaaga aaacaatgtc
     7501 attcgctcta atcagactga atcaatccaa caacgtctgg ctagcgacga tatcacttac
     7561 acggggaaga tttcgtctga gaacaagcaa ggctattatc ttagcgcgga gcagaccaat
     7621 cttagcactt cattagcgaa ttatcgcaag gctggtaaaa gcggcttgct ggcaagtggt
     7681 gtgtttttag acggtgatgt tttgacgaaa tctttatcca gttcagatgc tgaaaagaac
     7741 gtgatcgatc cagataaaat tagtagttcc ttggaagatt ttttatctaa caaagacaat
     7801 attttattcg gtaatgatta tgtgtacacg aaaaactttt ctcatttaga agaagaacca
     7861 ctagaaatcc aagcaagcca agagtatgaa ggcatcccat tcaatgatga tacagcaaaa
     7921 atcgttcttt ctttagaaaa aaacgataat gattatagca tcacgaaata catgcagacg
     7981 catttatctg atatcgagca gctgcgcgaa aagacggaac tgtatacgga agaggaagca
     8041 atcaatacat tgtacatcaa taacaaaata ccaagaggat caaaaatttt atggcgtcag
     8101 ctagcttatt cctgtattct gaaagtccgg gaaaaaaatg tgtatgttcc tgtttggcat
     8161 gtagcgatcg aaacaagcga taaagtggtc caaattgaga gcgtcaacgc tttcagcaat
     8221 acgatcgtga caaacaatac tgtaccaaaa gtggaagatc aataaaaaag cgagtataat
     8281 gaaaaaagtt tacaaagtaa gaagggaagt aggagtcaaa tggctgaaga aaaaccagct
     8341 tttaaaatca gtatcctagc aagtggaagt tcgggtaatt cactttatat cgaaagtgaa
     8401 aaaaaacggc ttttggtcga tgcaggactt agcggtaaaa aaatcacttc cttgatggct
     8461 caaattgatc ggacaccgga ccaattagat ggaatacttg tgacgcatga acatcgagat
     8521 catatacatg gcgtgggtgt cttggctcgt aagtatcatt tggatgtttt tgccaatcag
     8581 cagacttggg aagcgatgga accactgatt ggaaatgtac cagtggaaca aaaacatcta
     8641 tttgagatgg ggaaagtcag aacattcggc gatatcgata ttgagagttt cggcgtctcc
     8701 catgacgcag cggctccgca attttatcgt ttccataaag aaaatcggtc attcgtcatg
     8761 ttgacggaca ccggatattg cagtgattat atgcgaggtg tcattgaaaa tgccgatggt
     8821 tatttgatgg aaagtaatca tgatcttgag atgttacgca tgggacctta tccgtggaat
     8881 ttaaaacagc gtattttagg agatagagga catctttcca acgaagacgg tgcacttgtc
     8941 atgactgatg tgatcggtaa tcggaccaaa cgaatctatc ttggccatct tagcaaggaa
     9001 aacaatatga aagaacttgc tcatttaaca atggaaaatg ttttgaaaga aaaagatttt
     9061 ggcgttggac atgattttga aatctatgat acagatccag atgaagcaac tgagctattt
     9121 tcgatttaaa cagttcaatc aataaaaata taaaaagcag aagataaaat caatcagggt
     9181 tggttttatc ttctattttt atttttttag catattaaaa aaataaccgt attatcgact
     9241 gactacactg atgtgctgct tttcatgttt ctcatccagt atctcatcta ctaatgcaat
     9301 cgcgtaatct gccatgctga tcgtactgtc gctagcagcg tttctttgta aataatcatc
     9361 acttaacaaa taagtacctg tacgtttgcc atgtggatca aataaagaag ctggactgat
     9421 ataagtccaa tgtagatttt tagattgttt caattttaac aatccttcgt acatattgaa
     9481 agctgttgga tagaagggtg catttttagg tacttggtca atcatacgag tcgtcttttc
     9541 tgtatctaag aataacgaag aggcaccccc aacgacaatt aagcgtgtat cggttccttc
     9601 aagcatctca atcaaatggg acaaagaagt ttgatgcatt tcttcttttc ctttgggagc
     9661 gttgaaagca tcaacgacaa catcaaaaga tgcaagatca gtagttgtca actcaaataa
     9721 atccttttgt ataaacggaa catctgatgg caatttttct ggatgacgca cgattgccgt
     9781 cacgtctaaa tctctggaca atgcttcgtc taaaataaat gaacctgcat ggccagtagc
     9841 tccaatgagt gcaattttca taaaaaacct ccttttttat tcctgtccat tgtaagcagt
     9901 tgctgaatat tcgtcaaaga atccgatttt tgaaaagctc agtgcgcatt cctttcatgg
     9961 ttcgtgatca ttgggtatag ttaaattaag aaaactgatt gataaaaata gaagttgaag
    10021 gagataagaa atgaaagagt ggttagatcg tgccttttta ccaatgataa catctgttgt
    10081 gaatcttaga cccttaactg ctttgcgtaa tggaatgact tacacgatcc caataattat
    10141 tgttggctcg ctgtttttct tatttgctca ttttcctgtg gattacatcg ctcagtggat
    10201 agacaaaaca ggattttcta tgtattttac acaagtatac caggctacat ttgatgtgat
    10261 agccttttgg atcgtttttg ccattgcata cgcttatgca aaagaagaag acatcgatcg
    10321 cttaccagtc ggcataacag ccttgtctgc ttatctactg cttattcagc cactaaatac
    10381 tcagatagat actgtctgga caggtgctga agggatacta ggagcagtta caactgggct
    10441 catcatcgga tggggctatg cacgtttagt aagacttgcg aaagggtgga agcaaagaga
    10501 gaagatacct gaaaatgttt ggcagtcttt tacttcattg attccaatca ttgttatagt
    10561 cacttttacg cttatcggtt cagcggtttt tcaacatttg ttcagaatga ctgttgttca
    10621 aggagtatgg acatatgtcc agcttcccat tcaatcgttc atagacacgt tgtttggtgt
    10681 gcttttatta ggtttttttg ttccgttttt atggttattt ggtcttcatg gatctgcgat
    10741 ggtcaatggg ttggttagtc ccgtcttgca agcgaattca ttagcaaatg cagaaatctt
    10801 agcctcagga caagagttga cggtggcaaa tggcggacat atcgttactc agcaatttct
    10861 cgatcagttc atgacggtta cgggtgctgg attgacgtta ggggctgtat tttttatgac
    10921 ggtttttgcg aaatcgaaga aatacagaga attaggaaat cttgctcttt taccagcctt
    10981 tttcaacatc aatgaatcaa ttattttttc cacaccgatc gtgatgaacc cgatgatggc
    11041 tgttcctttt attttcgcac cggtcgtttc aggattgatt acctattccg ctttatattt
    11101 tggattcgtt cctttattta cagcagtcca agtcccttgg acaacaccgc cgatcctctc
    11161 gggtttctta gtaggcggag tgcctgcagc gattttacaa ggtatcgtgt tgtcaattag
    11221 ttttttcatt tatttcccat ttttgaaaaa aatagattct gcaaactgca aaaaggaaag
    11281 acagacaaaa aatgatggag cagcagaaaa gatgaacgac taaaaaacaa aaatactcct
    11341 gtcgctttag attgggaaaa tcaccaatct aggatgacag gagtatttat agtcatgttt
    11401 atactagccc tttttggaca gctaattgaa caagaacaac taatgtgttc attcctacgt
    11461 gtgtgatcat cgatgtccag atccgaccag ttgctttgta ctctaggctg aagaagaaac
    11521 ctaggaaaaa gtagattagc agatgaccat cattatgagc aaacgcaaac agcaaagagc
    11581 tgatcactgc tccgacccaa aaatttgaat atcgactgat gatccccagt atactgcggc
    11641 ggaatacgaa ctcttccatg atgggtccag caacagttgt cgccaaaata aaagcgggtg
    11701 cttccatgat catctggatg atattttgtg tattctcgga aggggttgct ttgccaaaca
    11761 atacagattc taacatgaca gcgatgcttt gcagaatcaa tgcgacaaag attccaacca
    11821 atccataaat aacaatcttt ggagcagaag ctttttttac cgctgattcg atcgctaagg
    11881 gttcacgttg cttaaaataa aaaatagcta ggacaacggc gcctagtata tacatgactg
    11941 cagttgctgt gatcaattca gacgtagatt ttacaagtcc tatcgaagct aggatcaagg
    12001 gtgagataaa aattagacca tagcaaaaaa tgctgaggaa gctatatttt tttaaagaca
    12061 tgtaattcct ccttttaagg gataacaagc atgcaaggca agcttatatc gattgatttc
    12121 aattatatca taatttcaat aagcagctgt cagaataaaa tttcttttct ttgatttaaa
    12181 agcttgcaaa aaagagggag aatgagtatt attaaaaatg taggttagca ctcgaaggtt
    12241 aagagtgcta acagattaaa aaaagagtta tcggagggat ttatcgtgtt aaaaccatta
    12301 ggtgatcgcg tcattatcga agtcgcaaaa gaagaagaaa aaactgtcgg aggaatcgtt
    12361 ttagcttctg ctgctaaaga gaaaccccaa actgggacag ttgttgcagt cggtgaaggc
    12421 cgtttgttag aaaacggtga aaaagttcct gccgctgtaa aagcaggcga ccaagttatg
    12481 ttcgaaaaat acgccggtac agaagtaaaa tacgaaggaa aagaatatct gatcgtcgca
    12541 ggaaaagata tcatggcgat tgttgagtaa atttggaaga taaaaaaata atcaaaaata
    12601 atcaatgagg tgaatcaatc atggcaaaag aattgaaatt tgcagaagac gcacgcgcag
    12661 ctatgttacg cggggtagat aaattagcag atacagtgaa agtaacatta ggacctaaag
    12721 gccgaaatgt cgtactagaa aaatcttacg gctctccatt gatcacaaat gatggtgtga
    12781 cgatcgcaaa agaaatcgaa ttagaagatc attttgaaaa catgggtgca aaacttgtat
    12841 cagaagtagc atcaaaaaca aatgatattg caggagacgg tacaacgaca gcaactgttc
    12901 tgacacaagc aatcgttcgc gaaggtttaa aaaacgtaac tgcaggtgct aatcctctag
    12961 gcattcgtcg cgggatcgaa ttggcaacaa aagcagcagt cgaagaattg cataatatct
    13021 caactgttgt tgattcaaaa gaagccattg cacaagtagc agctgtttct tcgggttctg
    13081 ataaagttgg acatttgatt gctgatgcaa tggaaaaagt tggaaacgac ggtgtcatca
    13141 caatcgaaga atcaaaaggg atcgaaacag aactagatgt tgtagaaggt atgcagtttg
    13201 atcgtggtta cttatcacaa tatatggtaa cagataacga caaaatggaa gctgttctag
    13261 aaaacccata tatcttgatc acagacaaaa aaatctctaa catccaagat attctgccat
    13321 tgttagaaca aatcttgcaa caatcacgtc cattgttgat tattgctgac gatgtagatg
    13381 gtgaagcatt gccaactctt gtattgaaca aaatccgtgg aacattcaac gtagtagctg
    13441 taaaagctcc tggtttcggc gatcgtcgta aagcaatgct tgaagatatc gctattttga
    13501 caggtggtac agtaattact gacgatctag gtttagaatt gaaagatgca acaatcgaaa
    13561 acttaggaaa tgcttctaaa gttgttgtag ataaagacaa tacaacgatc gtagaaggtt
    13621 ctggagaaaa agaagcaatc gaagctcgtg tgcaattgat caaaaaccaa attgctgaaa
    13681 caacttctga ttttgaccgc gaaaaattac aagaacgctt agctaaatta gcaggtggtg
    13741 tagctgttgt taaagttggt gcagctactg aaacagaatt gaaagaatta aaattacgca
    13801 ttgaagatgc attgaatgct acacgtgccg cagtagaaga aggaatggtc tcaggtggtg
    13861 gtacagctct agtgaacgtt atcagcaaag tttctgctgt agaagcagaa ggagacgtgg
    13921 caacaggtat caagatcgtt gttcgtgctt tagaagaacc aattcgtcaa atcgctgaaa
    13981 acgctggtta tgaaggatca gtgattgttg ataaattgaa gaacgtggaa ctaggcactg
    14041 gtttcaatgc tgctactgga gaatgggtaa acatggttga agcaggtatc gtagacccaa
    14101 ctaaagtaac acgttctgcc ttgcaaaatg ccgcttccgt ttctgccttg ttattaacga
    14161 cagaagcagt cgttgcagat aaaccagaac cagcagcgcc agcagctcct gcaatggatc
    14221 catcaatgat gggcggtatg atgtagtaat taagaaagaa ccctatttta acagggttct
    14281 ttcttctttt tttgttttat tttaagcaaa aggggcaaag aaggggcaaa aatatcaaag
    14341 gctatttagt ttatcaatta cttgttgttt ggctcttttt gttacatggt tataaataga
    14401 cagagttgtg tttgcatcag aatgccctac acgctccata atggctttaa gaggtacgcc
    14461 taattcagat aatagtgaaa cgtggctatg tctgaatata tgtgaggata gacttttttc
    14521 taattccaac tcttcctcta ctttatggag tatagcgtta aatgagtgca gcgcaagcgg
    14581 agtgccactt gtagatataa atatatattg atcgggatct gtgggtctac ctgcaagaat
    14641 attgtcagct atcacacttt caatcaattc ttttgcacga ttgggtaatt gcacttcacg
    14701 ttgcgaataa gtattttttg gagttgtttt tatagcatta tccattttca cagatgtgta
    14761 atctaaggtc ccattaatgg aaattttccc atcctcatag tccttcattt gcaaagctag
    14821 caattcccca tatctcaaac cagttaaata tagaaactca gctattatgc cgtgtagttt
    14881 tctgcgagga ttggagtata gctgtttcag tatttgatca atttcttctt tctccagata
    14941 ttttttattc atagaaagcc ttcttttttc ttcttccact tttttaggat ggattttaac
    15001 tgctagcgca gggtttcttt gaatgtattt tctatcgatt gcatagttta gcataacaga
    15061 tagagttgtt tttgtttgtt ttgtgtagtt cagtgagagg tcaccaaacg tatacatatc
    15121 ttcaattatc ttattaatga gtgtctcatc aatgtttcta acgatcgtat catcgcttat
    15181 gtgcttagaa acatgtttca tcatcattgg aaccttcaaa tagctagtac gtttaacatg
    15241 ctgcttataa tattcatacc attctttata cagctcacca aaagtgatat ctgatttatt
    15301 gtaatcttcg agtgcttctt taattttttt atctagaatt ttctgagctt ttttccacgc
    15361 ttgtggtgaa ttacttgtaa gtgttgtaga tttttttcgt gttttttctg tataaggatc
    15421 tacatatctt tcaataaact tgaattggcc atctttggtc tgttcaaccc acacttttaa
    15481 catctcctat catttgctat aataggcata acaaatagac ctatataggt ttgttttcta
    15541 aaagcacgct cttactctgg acggtggggc gtgtttttta tttattatac agttgcttta
    15601 aaaatagctg ctttttcaaa tgatactaaa ggagagaagt ggatttctat ttctcccttt
    15661 tggtttaacg tgaaatgagc agttacatcc atttgttttc ctggagcaac agatcccatt
    15721 gtattttcgt tggcatatgt ctctgatttt ttgtcatctg gtccatatac ttccacatct
    15781 gtacctacag ggatatctga atcgccatca ttttttacgg tataagtaat ttttactact
    15841 tgtgcaggtt ggttttcttc aaattgattt ctttcatcag ttagttctac actatttagt
    15901 gtatattcag catcaccaac ttttacagtg tcaccaatct tatagaaggt atcgcttttt
    15961 tcttctttag aagatgaagc agaggttgaa gatttagtta ctttttcgcc accattatca
    16021 cttgctttat tactatctga tccaccattt aaagcagaac caataattat aattaaaatt
    16081 actgctaata cccaaaacca tacttttttg taaaaaggtt tactaacttt atacattttt
    16141 ccgtcttgac ccataacttt ttttgccatt taaatattcc tcacttcttg ttataatata
    16201 tttgcgatct cagaaatgag gtatgagtcc gtgtgccagc acgggctttt ttaatgtttt
    16261 gctgaaatcg gtttttttaa taattcttga tgtattcata catattgcct tgcgtaagaa
    16321 tattttttct taaaatagca ttggcagaca gcataacaag tgtatctgag cttattctga
    16381 atagaataat actccataaa tttttcgaga ttaaactgag attcatcagt cagttcgttc
    16441 tcaatataga tatttaataa aattagaata gctattttat ctgcttctgt ttcaaatttt
    16501 gagtgaaaag ttgtagaagt atcgtacaaa actgaatatt caaaatgaga agcggtgaaa
    16561 tgcgcaagtt catgagataa atgaaaggct tctgcagttt cgccatatag attttcattc
    16621 aaaaaaatga ttctaggctt cggataatag aagccaggct ctttcatttc catgtaaact
    16681 actttcaaat tgtattcgct caacatttct ttcaatttca aatacataca acccatcact
    16741 ccaactattc attttcctct aaagctttag caattgcaat cgctttacgc attgtctcct
    16801 tagatatttc ttttccatca aaagaaaaaa cagtatcgtt ttctgataaa tccacatgtt
    16861 taggggtttc tctttcttct ctacctagaa ggtagtctac agagactcca aagtaatcag
    16921 ctaaccgagc taactcaata gaattaggag tttgtctttt ccaacttcct aaatatccat
    16981 tcgaataccc gaaattcatt tcgagctgtc taatggataa tcctttttct tttgctaact
    17041 cctttattat ttcgtaagta ttcattgata aatcaacctt tctgaatgct tacaaaaaaa
    17101 gtttagata
//
LOCUS       DAZULY010000036        16949 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000036 DAZULY010000000
VERSION     DAZULY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.036"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACG81A_000801"
     CDS             <1..1152
                     /locus_tag="ACG81A_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFS5014192.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG81A_000802"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG81A_000802"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFS5014193.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACG81A_000803"
     CDS             2728..3003
                     /locus_tag="ACG81A_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFS5014194.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACG81A_000804"
     CDS             3009..3776
                     /locus_tag="ACG81A_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFS5014195.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACG81A_000805"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACG81A_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFS5014196.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ACG81A_000806"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ACG81A_000806"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFS5014197.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ACG81A_000807"
     CDS             7920..8222
                     /locus_tag="ACG81A_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFS5014198.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ACG81A_000808"
     CDS             8225..9388
                     /locus_tag="ACG81A_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS5014199.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ACG81A_000809"
     CDS             9414..12842
                     /locus_tag="ACG81A_000809"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFS5014200.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ACG81A_000810"
     CDS             12863..13987
                     /locus_tag="ACG81A_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFS5014201.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13968..14414
                     /locus_tag="ACG81A_000811"
     CDS             13968..14414
                     /locus_tag="ACG81A_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFS5014202.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ACG81A_000812"
     CDS             14398..14736
                     /locus_tag="ACG81A_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFS5014203.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG81A_000813"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG81A_000813"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFS5014204.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ACG81A_000814"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ACG81A_000814"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFS5014205.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ACG81A_000815"
     CDS             15893..16936
                     /locus_tag="ACG81A_000815"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFS5014206.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT         5743 a         2932 c         3739 g         4535 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggttatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataatta
//
LOCUS       DAZULY010000037        16546 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000037 DAZULY010000000
VERSION     DAZULY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.037"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(23..847)
                     /locus_tag="ACG81A_000816"
     CDS             complement(23..847)
                     /locus_tag="ACG81A_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFS5014207.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(820..1500)
                     /locus_tag="ACG81A_000817"
     CDS             complement(820..1500)
                     /locus_tag="ACG81A_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS5014208.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1523..2461)
                     /locus_tag="ACG81A_000818"
     CDS             complement(1523..2461)
                     /locus_tag="ACG81A_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS5014209.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2706..3557)
                     /gene="ispE"
                     /locus_tag="ACG81A_000819"
     CDS             complement(2706..3557)
                     /gene="ispE"
                     /locus_tag="ACG81A_000819"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFS5014210.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3711..6284)
                     /gene="mprF"
                     /locus_tag="ACG81A_000820"
     CDS             complement(3711..6284)
                     /gene="mprF"
                     /locus_tag="ACG81A_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS5014211.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6425..7231
                     /gene="proC"
                     /locus_tag="ACG81A_000821"
     CDS             6425..7231
                     /gene="proC"
                     /locus_tag="ACG81A_000821"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFS5014212.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7310..8866)
                     /gene="rny"
                     /locus_tag="ACG81A_000822"
     CDS             complement(7310..8866)
                     /gene="rny"
                     /locus_tag="ACG81A_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFS5014213.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9277..10326)
                     /gene="recA"
                     /locus_tag="ACG81A_000823"
     CDS             complement(9277..10326)
                     /gene="recA"
                     /locus_tag="ACG81A_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFS5014214.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10435..11676)
                     /locus_tag="ACG81A_000824"
     CDS             complement(10435..11676)
                     /locus_tag="ACG81A_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFS5014215.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11916..12494)
                     /gene="pgsA"
                     /locus_tag="ACG81A_000825"
     CDS             complement(11916..12494)
                     /gene="pgsA"
                     /locus_tag="ACG81A_000825"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFS5014216.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12703..13626)
                     /locus_tag="ACG81A_000826"
     CDS             complement(12703..13626)
                     /locus_tag="ACG81A_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFS5014217.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14365..15651)
                     /locus_tag="ACG81A_000827"
     CDS             complement(14365..15651)
                     /locus_tag="ACG81A_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS5014218.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15644..>16546)
                     /locus_tag="ACG81A_000828"
     CDS             complement(15644..>16546)
                     /locus_tag="ACG81A_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS5014219.1"
                     /translation="QFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYFENSPEQ
                     KIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQLPFESRE
                     TVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFNGLFGGF
                     PHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQLESLRNG
                     EITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRMESVTIK
                     EIQQVAEQIELQAIFFLAGGNANE"
BASE COUNT         4709 a         3649 c         2891 g         5297 t
ORIGIN      
        1 taaggcttct tttctttata atttagttat tttttcttct gaaggcaacc aatattcgtt
       61 tgatgatatt tacaatcagg aacaaagcaa taaagactaa tgtgattgtt gctcctggag
      121 gtgtatctag ataaaacgag ctagttagtc ctgacagcat ccctagaaat cctacacaaa
      181 cactgatagc aatcacgaca ttgaagcttt tgcctaatcg cataccgatt gctgcgggaa
      241 gaaccatgat tgcggatatc aaaagtgcac cggcaatcgg gatcatcaca gcaatagcca
      301 caccagtaat cacattgaac atcatcgaca tcaaatgaac gggtagccca tccacgtggg
      361 cggtatcttc atcaaaagtc agaacataca tcggccgttt gaataaaaag aacaatatac
      421 cgatggcaac gaataagatt cctaaaaaga tgacttgctc ccatgtaatg gtcacgatcg
      481 aaccaaacag ataggattgg atacttgttg ctgaattccc tccgctcaaa ttcatcaata
      541 ctaacgctag cgccaaacca ccagccatta aaatggcaat ggagatttct gaataggttc
      601 ggtagatcgt tcgcagatac tccaatatca cagctgctaa tacaacgact agcatggtcg
      661 taagtgtagg attaaggttc aaaaagaatc ctaacgctac ccctgctaaa gagacatgag
      721 aaagcgtatc agccatcagc gattgtcttc tgatcactaa aaacacacct agcatcgggg
      781 cgattccggc aatgaagata gcggcaagaa acgctcttct catgaattca tatgaaagca
      841 acgccattga gaatcctcct ttctcaccag tcggatctgt ctgtccgcat actgtttgat
      901 atcttcatga tcatgggtga tcattagaac tgatttccca tggacatgcg cactatgtcg
      961 gagaagacga taaaattcat ttctggattc ttcatccata cctgttgttg gttcatcaag
     1021 aacaaataag tcagggtcag tagcaaaaat acgcgctaaa ctgattcgct gcttttgtcc
     1081 gccagacaat tcacctattc gccgatttcg catatcccac atccctacag cttctaaagc
     1141 acgctgtaca tgttgatgat ctttttcaga gagacgcttg aaccagcggc ctcttggata
     1201 tcttcctgaa cggactaatt cgatcaccgt actaggaaaa cctgcattga aagatgcaac
     1261 ttgttgagga atataaccga tacttaattt ttttccttct tgatttttct cagcaatctt
     1321 tatcattcct tttgaaggtt tcagtaatcc taacgatgct ttcaccaagg ttgacttggc
     1381 tgctccattt tctccagtta aaatgacaaa ttcacccgca tctacatgat aagagacatc
     1441 ttctagtaca ggttcatcat catagtaaaa ggtcaaatca gatatttcga tataacgcac
     1501 cttacaacct ccattctaaa acttaattga tactttcttt taaagctttc agattttttt
     1561 ccatgacaga aatataattt tcgcctgctt tgatttgttc attagtcaag ctttctaaag
     1621 gattcaacac ttctagcttc actcctgttt cattcgacaa tgtctcagct accttcgaag
     1681 aggcattttc ttcaaaataa atcactttga tgttgttttt cttcacaaat tcttttagct
     1741 cagctaagcg acttggtgtg ggttcctgat ctggtgaaac gcccgaaatc gctacttgtt
     1801 ttaagtcgta ttgattcgct aaataagcaa aagcagcatg ctgagtgaca aatgtgcggt
     1861 ttgttgcatc ttttagctca tcggagtatt tttgatccag tgcttcaagc tttttgatat
     1921 aagcatcggc attcttcgta aaagtttcct ttttatctgg atatttttcg cctaattctt
     1981 cagcaatcgt tctgacttct tttatagcta ataccggatc gagccaaaca tgtggatcgt
     2041 attcatgtga atgaccatgc ccttcttctg attcttcatg ttcgtctgct tcttgcgatt
     2101 ctaaacggtc gattccttta gctgcttcga tcatcaaggt ctgtttgcta tccactgaat
     2161 cctttaacga atccacaaaa aattccatgt ctgaactatt atacacgaaa acatctgcat
     2221 cggaaatttc tgctctttct tttgcacttg gttcgaaatc atgcggctcc gttccagcag
     2281 ggatcaaaag tttcacgttt ccttcatccc ctacgatctc ttttgtaaaa tcatacatcg
     2341 gataaaaagt cgtaacgatc gtcaaatcct cttttttgtc tgcttccttg ttcgtatttc
     2401 cacatgctgc aaacagtaaa gcacctatca ttaccgtagc tgtcaaaaga tattttttca
     2461 tttatatgct cctttcaatg aacaaatcgg aattttacga tttattaacg tgaactaatg
     2521 tatcaaaata aaaaaacaca gtcaatagaa atccatcaat ttctgattta agcatgtata
     2581 ttcttgatta aatcgtaaaa tggatgagtt gctgctcata tctagccatt tcactacaat
     2641 aaaaaactga agaataccat tttaggattc ctcagttttt catcacctat tttacttctt
     2701 atattttatt gcagggttcg tactaaataa acttcctcac aaaaaccttt taacccatta
     2761 tatactctct gcgctcttga ataatgttga cacaaggcaa aaacggttgg tccgcttcca
     2821 ctcatcaatg cagcatctgc tccgtacttg atcatacgct ctttgatttg atggatgact
     2881 ggatgccgct ttcccgttat atcttctaaa ctgtttccca tttttttgat catctgctga
     2941 taatcttgct ctcggatggc ttcagcaagt ccgtggatgt cttgatgttg gatggcttgc
     3001 gtatctactt ctctaaacac tgtacgtgta gagacactca ttcttggttt gaccaacaca
     3061 acccagcact gtggcataga aggtaatact tggatttctt cccctttgcc accgacaaat
     3121 gccgtacttc cgtagacaca gtaaggaaca tctgtaccga cttccaaacc gatttctgcc
     3181 agttcttcta acgaacagcc cagattccat aattggttga tccctcttag agcagctgca
     3241 cagtctgtac ttcctccgcc taatccagct gctactggaa tattcttctt aacaaagatc
     3301 ttgacaccgg ttttgatatt gtatttattt tttagcaata aagcagcttg ataaacattg
     3361 ttccgttcat ctaccggtaa aaatgcttta tttgtttcta caatgatttt gtcttctgtt
     3421 atttcttcaa aaaatagatg atctgccaag tctacgctcg acatcaccat ttccaactca
     3481 tgatacccat cttgccgtct atataataca tccagtccta aatttatctt ggctggcgct
     3541 ttttcaatca attccatcct ctcacccatt cgaaaagtct aagtcgcaag tattctatca
     3601 tagggaaaaa taaaagtata gcttatgtct gcctagtggt tgattttgtc atcgtttcaa
     3661 cagctttcac agaattttct cttcataatc caaacaagtt ctagtgattc ttataattct
     3721 gtgagcactt cttctgccaa actcggaaca tttccttttt cttttcctat cagactgagt
     3781 agctgcatgg tagcaaatcc gaaattactt cctttcctat aagcaaaata acgtccttcc
     3841 caatgatcga catattttaa tttcttttca taagcagact ggaaggaata gaaaaaatta
     3901 ccataattat atacgatatg catgactttt tcttgaaaga aagaaaaatc tgtttctcca
     3961 acattagcta atggagctaa gcctagattg gcttccgagt agcccatttt ctcgtataca
     4021 ataaacagat tggctaacag atagtcagct aattcctcag attctttagg taagacccgc
     4081 aacaaatcat atcccgcctg cttcttcgtc atcggctgag cggtgatgaa gccaacgact
     4141 ttttgatctt tacggacaat accgacaccg ctagataata catatgattt atcaaatcgt
     4201 cccacagaga agtatttttc tcgtgcacct tgaagccatt tttgtgaaac ttcatttaca
     4261 gcttgatgta attcttccgg cagttgatcg taccaagtaa aatggtatcc ttctttcatc
     4321 aaacgttgat attcaagttg ctgactcagc cgagacaagt cttctgttgc tagatcaaca
     4381 atccctgctt cacctacttt cgtgaagtga aaacccaatt catgaagcat cactacgtag
     4441 ctttctgata tcttataaaa cgcaagctga tagcctaaac aatctgcttg tttcataaac
     4501 tgattcgttg ctgcccgcat cattttagga ttgccaactg gatctcctaa aacaaagcat
     4561 ttgtttcctt tgatctgata accaaaaacg acttgatctt ggtcgtcttc ttggtaataa
     4621 aaataactgt aacctgacaa gcgaagctga tggcttgtag ctgtgcctcc gtatgtttcc
     4681 aataaacggt caaaacggtc tttttcgtac tcactgccaa gcttcgtagt atcttcagct
     4741 aaatattgat aaagcgccac taaagctagc aatgagatgc ccaaaccaat aagccctgag
     4801 aaccacacat catctgaagg aaacaaaata aaatgatttc ctgttattcg accgactaaa
     4861 aagcgtccgc tgtgatagcc tgcaattgcg taaacgataa acaaaaagcc gaacaagcta
     4921 gcatcaaaga ttatagctcc ccaagaataa acgaattttg ttcgataaaa ttcttttctg
     4981 gataaccaca cagctaacag aatcaagaca tataccaata tcaactgcca agataacgta
     5041 cgtgtgattg tatttaaaat accaaaagat agtaaagcaa tcgtaggcag aaatgccttt
     5101 ttgactttta ctgaaagtgc tctagcaagg ccgagtaata aaaagccaac caatagattc
     5161 actgtctgat ctaaaaaatt gaatgagaac ggcaataata cttgaaatag ccgactgatt
     5221 gcagacagat tagtgattgt tgatagaagt accatcatga tccccgcaaa atagacagca
     5281 cttacgagca cgatatgcgc aaccttttgg aaaaacagtc gcggaatgtt atctaaaaat
     5341 cgattgattt tgactcctgt atgtgtgatg aacaatagaa gtcccgtcat aaaaggtaga
     5401 acataataaa acaaccgata atataatagc caaactactg ctacatcttg ccgaacagac
     5461 aattgactca ttcctaagat catcaaaacg tcaaaagtcc ccattccacc tgggaccatc
     5521 gtcagcattc ctatcaatgt agcaatcatg aacataggat agatcgaaag catcgaaata
     5581 tctattctca tcaacttgcc gatgatcaaa aataccgcaa gggctcccgt ccattgtcca
     5641 aaggatgcca gtagcagacc aagcaagcct tttggataaa actcgaagaa caatgtcttt
     5701 ctctttagat aagcaaaaaa gaaaatgact ggtgctaaaa aactaccgcc gaaaagccag
     5761 atccaatagc ttcgaaaaat actttctgac tgaataaaaa agacatcaat aaatgtaaga
     5821 aacgaccata cagacaaacc agtcagcata aatagtgcga cttttgatac agtggctaaa
     5881 accatttttc tatttgtctg ctttccataa aaactagccc gcagacttgc tcctaccaca
     5941 ccaccaaaac cggcgagatt gttgatcgtg tttgttaccc acgcagcaag aagaaactct
     6001 tttctttcca tttttggttt tccttgtttt tctaagactc tgatcgtcac ccaatcatac
     6061 aacaacatcg gagtgacacc cagcagtcca gccagaatca tgagcaggat cgtcatgcgg
     6121 ctttgttgtc ccatcctttg ccaaatatct tgccaactca tgccattagc aatatttgcg
     6181 acttgatttg ccacaaagac taacacggag ccgaaaaaaa tcagttttaa aatcagacta
     6241 tgttccttta tccattgcag catcaagtat cgtttatttt tcacattttc ccattcctaa
     6301 cttgtatact tttgtgtcat accccaatgt tatcttattt attttaattt ttccacttta
     6361 gacctgttct ttgcttgcga tgatagaaaa agaaagatat cctaatgcta aaggagagga
     6421 taacatgaaa gtaggattta tcggcacagg gaatatggca caagctatta ttgatggttt
     6481 attaaaaaaa ggaacagtct cgaaagaaga ggtgttcgtt tccagtggac attttgaaaa
     6541 tgcacaaaaa tttgctgctg aaactggcgt ccatgcatgc aaaagtaatc aggaagttgc
     6601 atcacaatca gatatcgttc ttttagccgt gaaaccaaaa atcattgaaa aagtattgga
     6661 agaactaaga gaatatagcc agaaagtctt attcatttct attgcttcag gaaaatcatt
     6721 aatagattta gaaacagcgc ttgaagatcc tcaggcctcc atcgtacgtg tgatgccgaa
     6781 tgtcaatgtc agcgtaggag caggcgtttc tgcgatttgc aaaaatgaag ctgccacgga
     6841 ggaacagctt cagcaagcta cgatgatttt tgaagcaatc ggcagcgtct atcctttagc
     6901 agaatcagat ttcagtacat tcattggcct tgctggcagc tcacccgcat tcgtttacat
     6961 gctgatcgat gcgatgggac ggactggtgt actgaatggt ttgtccaaac agacagccac
     7021 tgagattgct gcaaaagcaa ttatgggttc ttgtcaaaaa ttcctttcaa gtgataaaaa
     7081 tccatgggaa cttgtagacc aagtttcttc tcctggcggt acaactgttg caggtgtagt
     7141 tgccttagaa gaagcaggat tcatcccagc aatcatcaaa ggaatcaata cgacgatcga
     7201 gaaagatctt gagatgttga ataaaaaata atgatagcaa tgttttttcg tgccttacaa
     7261 aaaaagaaga gctgtggctt ttgccgcggc tcttcttttg tattttttat tatttagcat
     7321 aatcagttgc acgagtttcc cgaataacga ttactttgat gtgtccagga taatccagtt
     7381 cttcttcgat tcgtttacga atatcacgta ctaaacgaac agcgtctaaa tccgagattt
     7441 cttctggctt gaccattacc cgaacttcgc gtccagcttg aactgcgtaa cttgattcta
     7501 caccggcgaa gctgttcgaa atattttcca gacttcttaa acgacggatg tagttttcta
     7561 atgattcact tcgcgcacca ggtcttgctg ctgagagcgc atctgctgca gctacaagga
     7621 cagagataac agaagtagct tccacatctc catgatgaga agcgatcgcg ttgattacta
     7681 ctgggttttc tttgtacttg gcagctaatt ccgcaccgat ctcaacgtga gatccttcaa
     7741 tctcatggtc aagtgctttc ccaatatcat ggagcaaccc ggcacgtttt gctaattgga
     7801 tatcttcgcc aagctctgct gctaagatac cagctaattt tgcaacctcg accgaatgct
     7861 tcaagacgtt ttgtccataa ctagttctga atcgcagacg tccgatgatc ttgattagat
     7921 ctggatgaag tgtatgagca ccgacttcaa acgctgcctc ttccccgtat tcgcgaatac
     7981 gttcatccat ttctttacga gatttctcga ccatttcttc gatacgcgct ggatggattc
     8041 gtccatcttg tatcagtttt tccaaggtca tccgtgcgat ttctcttcgg attggatcga
     8101 atccggaaag aacaactgct tccggtgtat catcgatgat taaatcgata ccagtcagcg
     8161 tctcaagcgt tcgaatatta cgaccttctc gtccaatgat acgacctttc atttcatcat
     8221 tcggaagggt aacgactgaa acagtcgttt ctgaaacttg atctgctgcg cagcgttgaa
     8281 tcgctaatga taataagttt ttcgctttgc gatctgcttc ttctttggca cgctgttcag
     8341 attcttttac cattagagtt aactcatgat tcagctcttc ttcagtcgat ttcatgatga
     8401 catctcgtgc gtcctctttt gataaaccag caattttttc tagttctttt tgttgatctt
     8461 caatcatttt ttcaacgtct ttttctcgct cttcaattaa ttgctgtttt gaactaagtg
     8521 cttcttcttt actttcaagt gagttctcgc gtttttctaa cgagtcatct ttacgattca
     8581 gcgtttgttc gcgttgaatc aaacgattct cttgagattt taattctaac ttactttctc
     8641 tcagctcact ttcaacttca gaacggtatt tctgattttc ttccttagct tctaacaatg
     8701 cttctttttt tagagtttct gcttcttttt ttgcactatc gatgattcca gcagctgatg
     8761 attgtgcacc agctatttct ttctcgtgac gtgactttgc gaccataaac cctaaaccaa
     8821 gaccgacaat taaaccgatg atagcgagga gaatgttgaa taccataaaa tccacctcca
     8881 tactatcttt ttcattttta tcatctatac agcttctatg ataaattatt ctaaactcgt
     8941 caatatgcat cttcgcatat atgtgtacaa aaaacatgca caactttatt ctaatgtttg
     9001 taccccgtag tgtcaacttt aaataaacaa agaaacggct ttctacttat tcattaaacg
     9061 atggttttta aaataaatga cactagtttc atataggtaa acctttaaat tgagaaactt
     9121 ttctattaaa ttattctttg tagatgaaca gatattttta ttttatgtac ccactcgtat
     9181 aattaaataa ctcacgattt ctttattcaa taaaaaaacg gcaggtcaag gagagtacct
     9241 actcttcaga ccagccgttt ttgtttatca taatttttat tcttctacta aaggcaattc
     9301 ttcttgtcct ttttcatctt ctgggacagc gacttcttca ccaataccat aagctgctct
     9361 aactagagca gaaacttctg ccatcatttc aggatgatta gccatataga ttttagcgtt
     9421 ttcccgtcct tgaccaatcc gatcttcttt ataagaatac caagcaccgc ttttatcaac
     9481 aatgtctttt tctactgcca tgtctagaag ttctccttct tgtgagatcc ctaaaccata
     9541 catgacatct acttcagcga ttttgaaagg aggtgctact ttgtttttta cgactttgat
     9601 ctttgtacgg ttaccaacaa tatctgtacc ttgtttcaat tgttctgcac gacgtacttc
     9661 caaacggatc gttgcgtaga atttcaatgc gcgtcctcca ggagtgattt ctggattccc
     9721 aaacatcacg cctacttttt cacggatttg gttgatgaaa atagcgattg tttttgtttt
     9781 gttgatcgaa ccagagagtt tacgcaatgc ttgagacatc aaacgtgctt gtaacccgac
     9841 atgtgagtca cccatttcgc cgtcgatttc tgctcgtgga actaaagcag caactgaatc
     9901 gactactacg atatctacag ccccacttga tactaaagca tcagcgatct ctagaccttg
     9961 ttctcctgtg tcaggctgtg aaagaagtag ttcatcgata ttcacaccta atttttgtgc
    10021 atattgcgga tctaacgcat gctcagcatc aatgaaagcg gccgttccgc catttttttg
    10081 tacttctgca atagcgtgta gtgcaactgt tgttttacct gaactttcag gtccatatac
    10141 ttcgatgata cgtccacggg ggtaaccgcc gacacctaaa gcgacatcta acgcaagaga
    10201 gccacttgga attgtagaga tttgttggtc gattttttcg cccaatttca taatagagcc
    10261 tttaccatag ctcttttcaa tttttttcag tgcagcatct aaggctgctt tacgatcatc
    10321 tgccaatacg ataatcctcc ttatatttac aaatcagagc aacatttcat tatccttatc
    10381 atatcttttt tagtgaaaaa aagcaagaaa aaaccgaaca aatattcgca tttatcattt
    10441 tcttaaaagc tgttttctca aaagatcaaa tccttgcata accgcattat atctgatagc
    10501 agcacgatct ctggcaaagt gatatttttc cgaaaccacg ccgtctttag atgaaatagc
    10561 gatccatact gttcctgcag attgaccttc taacgtgtcc ggtccagcca ctccggtgaa
    10621 agctacactg taatctgttc ttgctatctc acgtgcttga atggccatct gctccacaca
    10681 ttctttgctg actgttccat attctgctaa tagctccgga tcaatctcca gaaaaccagc
    10741 ttttgtttgg agcgagtagg taacgaatcc gcctggaaat acttcagata caccagcaat
    10801 gtcgcctaat gcggcttgga aggcgccggc tgtcaaactt tccgctgctg ttacagtctg
    10861 cttgttttct ttcagtagtt ccactacgac ttttgctagc gaattatcat cgccgtatcc
    10921 ataaaaatat tcccctactc gttcttggat tttttcttct aatgctaaaa gagcttgatt
    10981 ccctgcctga acatcatttg ttttcactgt cagtcgtaat gtcacttcat ttggtttggc
    11041 atatggtgcg atcgttggat tgatctgtgt ctcaatcaga tcttttagct cagtgaccag
    11101 tcgcgattca ccaatcccat aaaagcgcaa aactttagaa attagttttt cttcagaagg
    11161 aaaacgttcc tctagtaaag gacgtacttg ttccacaaac attggtttca actcattagg
    11221 cggaccggga agaaggatat acgcatgtgt atctgtttga taaaaagtcc ctaaagcaag
    11281 ccctgtacga tttggtagag gaaccccgcc ttcgataatc tgggactgtt gcagattgtt
    11341 tttggtcatt tttcgatggg ttgtttcaaa atatgccaat aattttttgt atccttccgt
    11401 attttgtatg agagacttac cgaggtgagc cgcagttgct tcttttgtca ggtcatcttc
    11461 tgtcggtcct aatccgccac ataagagtat gagttcactt cttgtttcag ctagagcaat
    11521 caactcttct aaacgctgct gattatctcc tacaactgtt tgataataaa cttcgatacc
    11581 tagatcagct agctgttctg acaagaaagt ggcattcgta ttcacgactt gtcctagtaa
    11641 aagttctgtt ccgacagcta ttatttctgc tttcattact tcccgctccc ttatatagta
    11701 tacgctgtat ttttatattt ttttatttaa aaagagggca aggagctgtg acaaaaatct
    11761 tgtcacagct ccttgttcca aataaacagt gttctcctgc caaaacctcg gtcataagtc
    11821 acaatataca taaaacatga aaagctgttt tttgaaatta tcccttgtga ctcagagtta
    11881 gacggcaggc tcacaacctc tccactaaac gatacttaca ttgatccttt gaacacaccg
    11941 ctgtttttga tgaagtaatc cacacctgaa tagatagtaa agatcagaca agcataaagc
    12001 atgatttgat ctactggaat accgatcagg ctgaatggga tattattgat aaacagcaag
    12061 ataatagcaa ccatttgtgt agccgtcttg acttttcccg gccaagctgc agccatgact
    12121 tctccatgtt cgacaagtag taaacgcaag cctgtgacgg ctaattcacg acagacaatg
    12181 atcgctacta cccaaccagg ggcctttcct tggtcaacca acatgataaa agcagtcata
    12241 actagcattt tatctgccaa cgggtccgca aacttgccaa aattagtgac aaggcctcgc
    12301 gcacgggcaa tttttccatc taaccagtct gtgatactag ctaaagcaaa aataactgca
    12361 cctactagct gtgtgacagc cagtgtggtg tctcctacgt taaattgtcc ccaatctaaa
    12421 ggtaaaccta caaccaaaat gaaaatcggg atcatacaaa ttctaagtac agttaattta
    12481 tttggtaaat tcacactatc gactcctttc ttccctattc tacgtgacta tcagcgaagt
    12541 ttccaccgat attatttact tttccaaatg aatttattca aaggagtatt tttattgaaa
    12601 ttgatctttt tgaattaacg aagaaggacc gcgacacaga tttgtcacga tccttctttt
    12661 ttatacctac aagtaaacag atgcgtaaac taatttatac gatcattctg cgtattgaag
    12721 agtaaggttc acattttttg gactctgatt gttttcccct ggattaactg gtacggattc
    12781 tccattcgct ttgatcgttg cattgcttgc tgcaccaatc gtgatggtcg catttgttgc
    12841 tgtttccggt aacacggtac tctgtgtttc atttgctgcc agtgtccctt gataaacata
    12901 ggcattgtct actagcacac ctaaccatac acgattgaca gcagtaaatt caagggttac
    12961 aggcttggta gcgtctgtga tatttactgc gatggcactg ccagaatctt ctcctgtagc
    13021 tattgccatt ttcttttctt cacttgattt tgttgttgat tctactgtag aagactcttt
    13081 ggtctgcgtg ctttgctctg aggaagagga ctcctcgact gatccttcta ctttaagcga
    13141 agaagctgtg tctccgatta ttggatcagc ttgacgatct tgccaagtca tatagcccac
    13201 gacaacgaca atcgccaaag caaccaatcc taataaaatg acaggaagac ttgtccaaaa
    13261 gcgacttgga tgcgattctt cctgatgcat tgcttttcta gaagtactta ctgtttcagg
    13321 ctgcggacgt tcagggaccg atcctgtcaa tttgcttttc ccatcaaaaa catcgactaa
    13381 atggtctcca tcttctccta cagcttcagc gtattgccga ataaatgccc gcacgtaaaa
    13441 tttaccaggc aattggtcaa aatcatcatt gtcgattgct tccaaataac gtttttgaat
    13501 ttttgtaatc tgctgtaatt catctaaaga tattttttta ttcaggcgtg cttgccgtaa
    13561 tttttctccg atggtttcaa aagccactcg ttcatcttct ccagtctgtt tgattctccg
    13621 aagcatctgc tgttgcaacg aaagacgtta tgtttgggtg ctgtgacaaa agtcatgtca
    13681 cagctccttt tttccgaata aacattgttc tcctgccaac tcctcggtca caagtcacaa
    13741 tatccaataa atataagaag ctgttttttg aacttgttcc ttgtgactcg gagttaaacg
    13801 gcaggctcac gacctctttc ttaaacggtg ttcaatcagc tgttcctcgg tattagctca
    13861 aaaaccggca aaatataagg agatattttt ccgtatgttt ctctaatact caggtcaaaa
    13921 cgctgatttc acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag
    13981 tccaaccaag ttaaaaacat taagaagctg tttttacttg gctgttctta ttacttgatg
    14041 cagggcagct ttttcacaac ctcttggtac acggtgttcc agaagtagct tcttcggcaa
    14101 taagccgaaa tatcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg
    14161 aagctgacca cttctgtcac aacctctttg attacttcgt ttgattttat tttaatataa
    14221 gttgattaaa agagagcatg atttgtttta ttttctcact aatttttcca acacaactaa
    14281 gaataccata aaagctgatt ttggaggagg ttcctactac aagtctaaaa cgaaatttaa
    14341 gacttttcct cataaaatac tggtttattt tggtctcatg taaaactcgc tgaaggcctc
    14401 ttcacttata aatgcttccc cagctgccag tacgtctttc atttggatcg aatcgatgat
    14461 ttcaggaaga tcaaataaag ttcgatcgcc aaataaactt tgcgtaaatt gattggcaat
    14521 atattcgatc gaatttaatg attggaaata ttgtccaagc attttcttct tcagcaaatc
    14581 gagatttgtt tctgacactt ccggatcatc agcgaatcca agaatgattt ctttgacttt
    14641 ttctgctgct ttctctggtt cgtccgtatc tccgctgaag tccgcaaagt gaaattcacg
    14701 atccaagctg aattcaaaac caaagctgtc atcgataatt ccttgattgt acatcgctag
    14761 atagtttcga gaagtattgc ctaaaatcat ttggaacaac agattgattg ctgttttata
    14821 gcgtaacagt tctgttcctt cttgcggcaa tgtgtctaat cctttaatac caagtacaaa
    14881 tttatctcgt gtaatggctg cttccaatgc actttgtttg atgatttctt ttgtgttttc
    14941 agggaaataa cgtacaattt cttgctttgg agggaaattt tttgcttctt ggttttcccg
    15001 aataagtttc atcaatttct caggctccat cttaccaact acaaataaaa ccatattgct
    15061 tggctgataa aacgtccgat agcacgtata caagtcttga gctgtgattt tgtcgatact
    15121 ttcaaccgtg cccgcaatat cgatatgcaa tgggtgtgta ggatacaagt tatttagtat
    15181 gccaaaaaac atccgccagt tagggtcgtc ttcatacatt tggatttcct gaccgatgat
    15241 tcctttttcc ttgtttacag tttcttctgt gaagtaaggt gcttgtacaa aatcgatcag
    15301 agtcgtcaga ttcttttcta cctgatccgt cgtagaaaac agatagctgg ttttcgtaaa
    15361 acttgtgaaa gcattggcgg aagctccttg tttcccaaat aattgaaaga catcaccatc
    15421 ttctttttca aacaacttat gttctaaaaa atgggcaatt ccatctggaa ctttcacctt
    15481 ttctttttca ccatagggaa taaattcgtt atcgatcgaa ccataattcg tactgaacaa
    15541 accatatgtc ttgtgatagt catttttagg caacaaatac acggtcaaac cgtttggcag
    15601 tacttcatga taaagtgttt cattgatttg ctcatattct gttttattca tttgcatttc
    15661 ctcctgctaa aaagaagatt gcctgcaatt cgatttgctc agctacttgt tgtatctctt
    15721 tgatcgttac gctctccata cgtttcaacc attcttcatc agataatttt gtttccggca
    15781 gccagctgtc caaaaaagaa gcttcgattg ccgcttgcgg agagtctaga gaaagcagat
    15841 attggtttct cagcattgct tttgtctgag acagttccaa ctctgttatt tcaccatttc
    15901 gcaaactttc taattgctcg tgaatcagat gaagtacttg atttcggttc ttttcatcga
    15961 taccggtttg gacactcatg aatcctcgga acgtatccac gctactagag gcataataag
    16021 ctagactttc tttctctctg acattcatga aaagtttaga gtgaggaaaa ccgccaaata
    16081 aaccgttaaa gaccattagc gcaaaacgtt ctggttcatc gtaatacaca tttgtctgat
    16141 aagctaggtt cagctttgct tgaacgattg gctcacgtac ttgtcgttcc ttcacgatat
    16201 tcacttgagg ttgggtataa aacagatctg gataaactgt ttcccgcgac tcaaatggta
    16261 attgcccaac tgcttcagct gctttgtccg gatcaacatc gccgacaaca aaaatgtcga
    16321 tttgatcatt tgccatcatt tcttgatagt aggacgctag cgaactagca gtgatcttgt
    16381 ccaaatcttc gactacgccc aaccctggga tcttttgttc cgggctgttt tcaaaataaa
    16441 gttcttgcaa ggcataggaa gcaaaagttt gtttatcttc cttcaaactt tccaaatata
    16501 gacgcagatt gttcttttcc aagtcaaacg tcaactgatc aaactg
//
LOCUS       DAZULY010000038        16465 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000038 DAZULY010000000
VERSION     DAZULY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.038"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(29..727)
                     /locus_tag="ACG81A_000829"
     CDS             complement(29..727)
                     /locus_tag="ACG81A_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFS5014220.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(720..2072)
                     /locus_tag="ACG81A_000830"
     CDS             complement(720..2072)
                     /locus_tag="ACG81A_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFS5014221.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2295..3695)
                     /locus_tag="ACG81A_000831"
     CDS             complement(2295..3695)
                     /locus_tag="ACG81A_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS5014222.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3856..4593
                     /locus_tag="ACG81A_000832"
     CDS             3856..4593
                     /locus_tag="ACG81A_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFS5014223.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4718..5197
                     /locus_tag="ACG81A_000833"
     CDS             4718..5197
                     /locus_tag="ACG81A_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFS5014224.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5194..5961
                     /locus_tag="ACG81A_000834"
     CDS             5194..5961
                     /locus_tag="ACG81A_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFS5014225.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6191..6760
                     /gene="lepB"
                     /locus_tag="ACG81A_000835"
     CDS             6191..6760
                     /gene="lepB"
                     /locus_tag="ACG81A_000835"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS5014226.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6864..7466)
                     /locus_tag="ACG81A_000836"
     CDS             complement(6864..7466)
                     /locus_tag="ACG81A_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFS5014227.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7572..7802
                     /locus_tag="ACG81A_000837"
     CDS             7572..7802
                     /locus_tag="ACG81A_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014228.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7793..8041
                     /locus_tag="ACG81A_000838"
     CDS             7793..8041
                     /locus_tag="ACG81A_000838"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5014229.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8147..8731
                     /locus_tag="ACG81A_000839"
     CDS             8147..8731
                     /locus_tag="ACG81A_000839"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFS5014230.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8734..9807
                     /gene="prfA"
                     /locus_tag="ACG81A_000840"
     CDS             8734..9807
                     /gene="prfA"
                     /locus_tag="ACG81A_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFS5014231.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9800..10639
                     /gene="prmC"
                     /locus_tag="ACG81A_000841"
     CDS             9800..10639
                     /gene="prmC"
                     /locus_tag="ACG81A_000841"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFS5014232.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10632..10811
                     /locus_tag="ACG81A_000842"
     CDS             10632..10811
                     /locus_tag="ACG81A_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014233.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10833..11858
                     /locus_tag="ACG81A_000843"
     CDS             10833..11858
                     /locus_tag="ACG81A_000843"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFS5014234.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12033..13277
                     /gene="glyA"
                     /locus_tag="ACG81A_000844"
     CDS             12033..13277
                     /gene="glyA"
                     /locus_tag="ACG81A_000844"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFS5014235.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13430..14377
                     /locus_tag="ACG81A_000845"
     CDS             13430..14377
                     /locus_tag="ACG81A_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFS5014236.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14511..15140
                     /gene="upp"
                     /locus_tag="ACG81A_000846"
     CDS             14511..15140
                     /gene="upp"
                     /locus_tag="ACG81A_000846"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFS5014237.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15279..15521)
                     /locus_tag="ACG81A_000847"
     CDS             complement(15279..15521)
                     /locus_tag="ACG81A_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014238.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15642..15714
                     /locus_tag="ACG81A_000848"
     tRNA            15642..15714
                     /locus_tag="ACG81A_000848"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15675..15677,aa:Thr,seq:cgt)
     gene            complement(15833..>16465)
                     /locus_tag="ACG81A_000849"
     CDS             complement(15833..>16465)
                     /locus_tag="ACG81A_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFS5014239.1"
                     /translation="NILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITGFI
                     AFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPINE
                     AFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNGNI
                     LHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         5612 a         2941 c         3314 g         4598 t
ORIGIN      
        1 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
       61 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
      121 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
      181 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
      241 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
      301 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
      361 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
      421 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
      481 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
      541 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
      601 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
      661 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
      721 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
      781 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
      841 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
      901 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
      961 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
     1021 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
     1081 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
     1141 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
     1201 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
     1261 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
     1321 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
     1381 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
     1441 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
     1501 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
     1561 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
     1621 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
     1681 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
     1741 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
     1801 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
     1861 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
     1921 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
     1981 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
     2041 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
     2101 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
     2161 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
     2221 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
     2281 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
     2341 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
     2401 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
     2461 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
     2521 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
     2581 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
     2641 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
     2701 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
     2761 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
     2821 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
     2881 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
     2941 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
     3001 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
     3061 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
     3121 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
     3181 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
     3241 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
     3301 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
     3361 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
     3421 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
     3481 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
     3541 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
     3601 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
     3661 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
     3721 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
     3781 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
     3841 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
     3901 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
     3961 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
     4021 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
     4081 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
     4141 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
     4201 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
     4261 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
     4321 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
     4381 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
     4441 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
     4501 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
     4561 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
     4621 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
     4681 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
     4741 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
     4801 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
     4861 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
     4921 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
     4981 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
     5041 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
     5101 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
     5161 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
     5221 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
     5281 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
     5341 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
     5401 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
     5461 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
     5521 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
     5581 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
     5641 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
     5701 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
     5761 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
     5821 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
     5881 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
     5941 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
     6001 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
     6061 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
     6121 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
     6181 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
     6241 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
     6301 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
     6361 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
     6421 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
     6481 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
     6541 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
     6601 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
     6661 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
     6721 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
     6781 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
     6841 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
     6901 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
     6961 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
     7021 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
     7081 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
     7141 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
     7201 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
     7261 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
     7321 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
     7381 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
     7441 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
     7501 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
     7561 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
     7621 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
     7681 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
     7741 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
     7801 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
     7861 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
     7921 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
     7981 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
     8041 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
     8101 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
     8161 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
     8221 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
     8281 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
     8341 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
     8401 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
     8461 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
     8521 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
     8581 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
     8641 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
     8701 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
     8761 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
     8821 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
     8881 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
     8941 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
     9001 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
     9061 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
     9121 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
     9181 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
     9241 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
     9301 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
     9361 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
     9421 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
     9481 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
     9541 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
     9601 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
     9661 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
     9721 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
     9781 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
     9841 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
     9901 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
     9961 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    10021 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    10081 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    10141 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    10201 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    10261 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    10321 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    10381 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    10441 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    10501 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    10561 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaacgaat
    10621 gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt tttattataa
    10681 aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa gtttatccac
    10741 tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc agacaaaata
    10801 agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat gatgaaacag
    10861 aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt cctaccgaaa
    10921 cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa gtgttttctg
    10981 taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt gaacaagtaa
    11041 aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac ttctggcctg
    11101 gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt gtgacgggag
    11161 gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa gtaatcgaac
    11221 tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca agtccaacga
    11281 ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt gatgatggag
    11341 ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca gccatgccta
    11401 tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc gaatctcctg
    11461 ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct gggatgaaat
    11521 acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat tggtcaactg
    11581 cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc cccgaaattg
    11641 cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac tcagtagaag
    11701 cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca cttggattag
    11761 atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac atgaatcgct
    11821 tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat tgtcgcattc
    11881 ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa aactaagcgt
    11941 aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg ggaaaaataa
    12001 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    12061 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    12121 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    12181 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    12241 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    12301 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    12361 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    12421 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    12481 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    12541 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    12601 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    12661 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    12721 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    12781 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    12841 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    12901 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    12961 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    13021 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    13081 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    13141 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    13201 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    13261 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    13321 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    13381 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    13441 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    13501 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    13561 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    13621 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    13681 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    13741 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    13801 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    13861 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    13921 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    13981 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    14041 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    14101 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    14161 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    14221 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    14281 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    14341 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    14401 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    14461 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    14521 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    14581 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    14641 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    14701 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    14761 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    14821 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    14881 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    14941 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    15001 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    15061 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    15121 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    15181 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    15241 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    15301 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    15361 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    15421 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    15481 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    15541 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    15601 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    15661 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    15721 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    15781 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    15841 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    15901 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    15961 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    16021 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    16081 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    16141 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    16201 gttgcaaaaa taccgagact gcggcactga ttactacatt gataaaaaaa tatttggcta
    16261 tatttttgac aggagcgaca ggtttcgaga acattttttt cgtatctttt ttaccaaaaa
    16321 caagtgtaaa tgctataaat ccagtgatag ttaaagaaaa aatgctaata atggatggcg
    16381 caaatagcaa ccctaacaaa ccgagtacaa cgacaatagt gcccaatatc ccttttttaa
    16441 gttctatttt ttttcctaaa atgtt
//
LOCUS       DAZULY010000039        16382 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000039 DAZULY010000000
VERSION     DAZULY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.039"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..550)
                     /locus_tag="ACG81A_000850"
     CDS             complement(152..550)
                     /locus_tag="ACG81A_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFS5014240.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="ACG81A_000851"
     CDS             complement(547..1320)
                     /locus_tag="ACG81A_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFS5014241.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="ACG81A_000852"
     CDS             complement(1317..2000)
                     /locus_tag="ACG81A_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFS5014242.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="ACG81A_000853"
     CDS             complement(2167..3054)
                     /locus_tag="ACG81A_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5014243.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="ACG81A_000854"
     CDS             complement(3051..3920)
                     /locus_tag="ACG81A_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5014244.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="ACG81A_000855"
     CDS             complement(4057..5343)
                     /locus_tag="ACG81A_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5014245.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="ACG81A_000856"
     CDS             5616..6194
                     /locus_tag="ACG81A_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5014246.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="ACG81A_000857"
     CDS             6194..7123
                     /locus_tag="ACG81A_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5014247.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="ACG81A_000858"
     CDS             7120..8856
                     /locus_tag="ACG81A_000858"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5014248.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACG81A_000859"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ACG81A_000859"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFS5014249.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="ACG81A_000860"
     CDS             complement(9752..10261)
                     /locus_tag="ACG81A_000860"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFS5014250.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACG81A_000861"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ACG81A_000861"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFS5014251.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="ACG81A_000862"
     CDS             complement(11562..12653)
                     /locus_tag="ACG81A_000862"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFS5014252.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACG81A_000863"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ACG81A_000863"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFS5014253.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACG81A_000864"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ACG81A_000864"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFS5014254.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACG81A_000865"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ACG81A_000865"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFS5014255.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4728 a         3621 c         2912 g         5121 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aa
//
LOCUS       DAZULY010000040        16366 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000040 DAZULY010000000
VERSION     DAZULY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.040"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="ACG81A_000866"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="ACG81A_000866"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFS5014256.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="ACG81A_000867"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="ACG81A_000867"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFS5014257.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="ACG81A_000868"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="ACG81A_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFS5014258.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="ACG81A_000869"
     CDS             2857..3732
                     /locus_tag="ACG81A_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFS5014259.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ACG81A_000870"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ACG81A_000870"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFS5014260.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="ACG81A_000871"
     CDS             4629..5063
                     /locus_tag="ACG81A_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFS5014261.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="ACG81A_000872"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="ACG81A_000872"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFS5014262.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="ACG81A_000873"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="ACG81A_000873"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFS5014263.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="ACG81A_000874"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="ACG81A_000874"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFS5014264.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="ACG81A_000875"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="ACG81A_000875"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFS5014265.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="ACG81A_000876"
     CDS             13334..14260
                     /locus_tag="ACG81A_000876"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFS5014266.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="ACG81A_000877"
     CDS             14442..15335
                     /locus_tag="ACG81A_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFS5014267.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="ACG81A_000878"
     CDS             15469..15594
                     /locus_tag="ACG81A_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFS5014268.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="ACG81A_000879"
     CDS             complement(15728..16360)
                     /locus_tag="ACG81A_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014269.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         5812 a         2789 c         3492 g         4273 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatc
//
LOCUS       DAZULY010000041        16280 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000041 DAZULY010000000
VERSION     DAZULY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.041"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..1371)
                     /locus_tag="ACG81A_000880"
     CDS             complement(61..1371)
                     /locus_tag="ACG81A_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFS5014270.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="ACG81A_000881"
     CDS             complement(1383..2801)
                     /locus_tag="ACG81A_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5014271.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="ACG81A_000882"
     CDS             complement(3041..4609)
                     /locus_tag="ACG81A_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFS5014272.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="ACG81A_000883"
     CDS             complement(4613..4894)
                     /locus_tag="ACG81A_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFS5014273.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="ACG81A_000884"
     CDS             complement(4891..5814)
                     /locus_tag="ACG81A_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFS5014274.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="ACG81A_000885"
     CDS             complement(5811..6689)
                     /locus_tag="ACG81A_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFS5014275.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="ACG81A_000886"
     CDS             complement(6676..8013)
                     /locus_tag="ACG81A_000886"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFS5014276.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="ACG81A_000887"
     CDS             complement(8016..9119)
                     /locus_tag="ACG81A_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFS5014277.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="ACG81A_000888"
     CDS             complement(9193..10701)
                     /locus_tag="ACG81A_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5014278.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="ACG81A_000889"
     CDS             complement(10667..11359)
                     /locus_tag="ACG81A_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFS5014279.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="ACG81A_000890"
     CDS             complement(11381..12355)
                     /locus_tag="ACG81A_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5014280.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="ACG81A_000891"
     CDS             12655..13527
                     /locus_tag="ACG81A_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5014281.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..>16280)
                     /locus_tag="ACG81A_000892"
     CDS             complement(14109..>16280)
                     /locus_tag="ACG81A_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5014282.1"
                     /translation="IFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTDKKNLLRQVK
                     TNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFIETIVMDDNN
                     YDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIEMVISLPMFE
                     KEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQPTVLTDVDLD
                     YNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAVSLTLVGLIG
                     LFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEALSAHLVTQK
                     PQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLEDSHGGERDLL
                     LLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYCRLIQKRVKE
                     YLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIVPAAHEKTLI
                     RYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRLINELVQLGQ
                     LLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITDHEFKNLSEN
                     IMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHRLEVAKKWLK
                     ETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
BASE COUNT         4788 a         3428 c         2655 g         5409 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat
//
LOCUS       DAZULY010000042        15973 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000042 DAZULY010000000
VERSION     DAZULY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.042"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(155..781)
                     /locus_tag="ACG81A_000893"
     CDS             complement(155..781)
                     /locus_tag="ACG81A_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014283.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1076..1411)
                     /locus_tag="ACG81A_000894"
     CDS             complement(1076..1411)
                     /locus_tag="ACG81A_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS5014284.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1398..1682)
                     /locus_tag="ACG81A_000895"
     CDS             complement(1398..1682)
                     /locus_tag="ACG81A_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFS5014285.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1738..3261)
                     /locus_tag="ACG81A_000896"
     CDS             complement(1738..3261)
                     /locus_tag="ACG81A_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS5014286.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3254..4429)
                     /locus_tag="ACG81A_000897"
     CDS             complement(3254..4429)
                     /locus_tag="ACG81A_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS5014287.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4433..4618)
                     /locus_tag="ACG81A_000898"
     CDS             complement(4433..4618)
                     /locus_tag="ACG81A_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014288.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4584..6278)
                     /locus_tag="ACG81A_000899"
     CDS             complement(4584..6278)
                     /locus_tag="ACG81A_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS5014289.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6275..6748)
                     /locus_tag="ACG81A_000900"
     CDS             complement(6275..6748)
                     /locus_tag="ACG81A_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFS5014290.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(6817..6972)
                     /locus_tag="ACG81A_000901"
     CDS             complement(6817..6972)
                     /locus_tag="ACG81A_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFS5014291.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7106..7486)
                     /locus_tag="ACG81A_000902"
     CDS             complement(7106..7486)
                     /locus_tag="ACG81A_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS5014292.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7490..7705)
                     /locus_tag="ACG81A_000903"
     CDS             complement(7490..7705)
                     /locus_tag="ACG81A_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014293.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7708..8115)
                     /locus_tag="ACG81A_000904"
     CDS             complement(7708..8115)
                     /locus_tag="ACG81A_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFS5014294.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8381..9856)
                     /locus_tag="ACG81A_000905"
     CDS             complement(8381..9856)
                     /locus_tag="ACG81A_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFS5014295.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(9846..10694)
                     /locus_tag="ACG81A_000906"
     CDS             complement(9846..10694)
                     /locus_tag="ACG81A_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFS5014296.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10731..11093)
                     /locus_tag="ACG81A_000907"
     CDS             complement(10731..11093)
                     /locus_tag="ACG81A_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014297.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11094..11252)
                     /locus_tag="ACG81A_000908"
     CDS             complement(11094..11252)
                     /locus_tag="ACG81A_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014298.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(11393..12017)
                     /locus_tag="ACG81A_000909"
                     /pseudo
     CDS             complement(11393..12017)
                     /locus_tag="ACG81A_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12014..12325)
                     /locus_tag="ACG81A_000910"
     CDS             complement(12014..12325)
                     /locus_tag="ACG81A_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014299.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12369..12662)
                     /locus_tag="ACG81A_000911"
     CDS             complement(12369..12662)
                     /locus_tag="ACG81A_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014300.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            12856..13503
                     /locus_tag="ACG81A_000912"
     CDS             12856..13503
                     /locus_tag="ACG81A_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5014301.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13564..14709
                     /locus_tag="ACG81A_000913"
     CDS             13564..14709
                     /locus_tag="ACG81A_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5014302.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(14790..15095)
                     /locus_tag="ACG81A_000914"
     CDS             complement(14790..15095)
                     /locus_tag="ACG81A_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFS5014303.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(15120..15551)
                     /gene="ruvX"
                     /locus_tag="ACG81A_000915"
     CDS             complement(15120..15551)
                     /gene="ruvX"
                     /locus_tag="ACG81A_000915"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFS5014304.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(15548..15814)
                     /locus_tag="ACG81A_000916"
     CDS             complement(15548..15814)
                     /locus_tag="ACG81A_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFS5014305.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
BASE COUNT         4580 a         3317 c         2538 g         5538 t
ORIGIN      
        1 atgaatgaga aacccatatc aaagaactat aaaggttatc aaaacagcgt ttatgactaa
       61 atgaattaga gttatctatc actatttttc aagcgattta aaccaaatgg catacaatat
      121 ggcatacaaa aacttcaata agccgtgggg gtttttaaat ttcttcttgt tctttaaaat
      181 caatgagtgg agctacataa tttgaatcca ctttttcaaa cgtcactttt tgcatcgacg
      241 aatgttttct ttcccaagat aacgtaaata gatttgactc tggacttata aagtctactt
      301 caccttctag tattactttc ttatgcaaat attcctctaa taaatataga cttgctaaag
      361 catacaacac attttccaaa tttgcttcct tataatttga agaacgacca tgttttactc
      421 tgttatatat tttccaccaa tctggtgtat cttcaggcca cccatctcca aaaggtttta
      481 gtgaagtcaa tgttaaagaa ttcataaccc taacaccatt atccaaacac attaattcgc
      541 tagatttaat aacagcttta aaatcagtta tgcttttatt gctactgcta tcaaatccgc
      601 aaagttcttt gaaaataatc tcacactccg aaccaatcgc tagtagtagt ttttgatatt
      661 caatagagaa tgttttgaaa ttattctcat cgagcatcac aaaatctgtg gtattaataa
      721 actcattttc taaatgtaga taatacttcc aataaaaatt tgtaaactct gctttgtcca
      781 ttaaatcacc tcttttgaat tataacaaaa aaaacacgtc cacaacagac gtgttctttg
      841 atttctacac aatcagcttg ccaaaaccat ccaatcagtc ctagcatgcc tgtattaaaa
      901 ttgaacgttc atcatctatc atctttctta gtccagcatg tttgcaacca cacctacaat
      961 tttcagtcca acaatagatt tcgactagct aagtcttgca aaattcaggc agctaaccct
     1021 tcatttacta ccttaattat actaaatacc cacaacgttt acaagtgtaa tcattctaag
     1081 aaatctgttt agctatgatc gttgtataat ctttaaacgc cgttccttca ttgattgaaa
     1141 taattttata actcttgttt ttccaagcta ctttcatatc gtttgttagt tcataatctt
     1201 gatcgtatcg aataataaac gtcaacgttc cttcaagaat ggtacctaca ctagcaagca
     1261 cgtcttttag catttgtgaa cgcacattag cccaacattc aaatacagtt tcttcactat
     1321 ccaccatctg cccatcttca tcttttaccc gttttgtctt tttaaacttg actctctcag
     1381 tcaattcatt tacattctta atgattggca taataagcac ctctcatttg ttgtactaat
     1441 gcttgtaccg ttactggtat ccgttcgctg ctggcttctt gacggttcaa ataccaatgt
     1501 tgagtaagta atgacaccgc caaatcaaat tgctgataat tatccatatc tttatccgtt
     1561 gcgctactat caatagcact ttttatatat tcgcctgctg tatcaatcaa ttgttgaata
     1621 aagtcatcat caatagtgtg atcgatccgc atactttttt ttatgctatc caaaattacc
     1681 attttatctc tcctaatgaa aaaaaggcag tgggataaac cgccgcccaa tatttttcta
     1741 tacttttggt gttacaactg gcgtaaactc aatatacact gaagcatttt gatcaatttt
     1801 cttataatcg ttgcgaacga tcactgcaag tccttgtgag tagtaatcga atttttccca
     1861 ttgagtcgtt acttgatttc tacgagcaac aaagatagat tgtgcaatat cccccataat
     1921 catagggaat gttccagctt taggattggc aaacaatgta tctgcaatca atactactgg
     1981 cataccgaat aatgatttac cactaggtgc tgtcacatcg ggttgtaaaa tgtaacgtcc
     2041 atctgaatct ttcaatgtat ctaggtggta atatccgctt tggtttagaa ttaccatttt
     2101 atttaatgct gggtctaatg ccacattgta tagttgtttc aaatcgtcca acgtagcagc
     2161 cgttttctta gtgaatgttt ttaataaatc catgatatgc ttattgtcag tgttttctac
     2221 caatttcgct aactgatctt tgacctcttg tacaatattc actgctgaat cttccacaac
     2281 ctcatttgat aaggcaatct taccagcacg agtttctact ttataatcaa ctgaagtaaa
     2341 catttcagcg tcaatatcac caatttcagc aagttcagct tttgttgcta acactgcttg
     2401 ttggttagtt gctactggat atttaccttg tccatttgat actgttttta ctgttgcata
     2461 ttgagctaag ttataatttg aacgtttcaa atcaaagact tcaccgatta cttcttcggg
     2521 aacaactgcc gctgcattta cagtagtgac tccatcacgc acttcgcctt gtgaacggat
     2581 atattcttca tacccacgca attctgtttt ttcgttatcg attaaagttt tttccatgtt
     2641 attactctcc ttttgtgctt tagaagtgga ttctaagcta ttgtttgttt gttttgcttg
     2701 gttgttactc ataaacgatt cgtaagaacg cttatttact tggacatttg atgaatcata
     2761 agcgggaacg gtcacgacac taatttcatt aagtgctttg attttatcta ttgatcgttc
     2821 aattgtgcca tcttcttttt tgtcgaatga atctaatcca agcacgaacc caaaggacat
     2881 agaatcaaca accccttttg agatattctc atacacatca tttgaatagg tcgtgtcggt
     2941 tagctctgct tcaaaatgta atcctgtatc atcgacattc aatttcaacg tacctgcttt
     3001 gacactagct agtggctttg aataatcgtg tccatataat aagaacacat ttgataaatc
     3061 cacctctttt aatgcttcgg gtgtaatcac ttctacaaag ccgcctaaat cttttgacgg
     3121 ttgcccgaat ttcaacgcat agcctgaaat agttttgccg tctgttggtt gtttttcatt
     3181 ttctttctct actttgggag aatcggctgt tagctcagct tcttctgtca gccgtttttc
     3241 cttttcttct tcattattca actggtgtaa ctccttcctg ttcttttaat tgatatttct
     3301 ccaacgtatc taaatacgta aagttcaaac ttgctagtaa acgatcgcca ccatccatgg
     3361 ggggtagccc cattttcgac cgcccctcat taatcgtgag aagtgaccct ttgacctgat
     3421 ccaatacatt ttttactttt gtttcaggat cagtctctag taactgatcg gaattaaagc
     3481 ggatattggt ttcaagtttc gtatctaatt cactcacaaa cacattaaag taatgaatca
     3541 gcgtactttg gatatactgg agattacttt gaacggtgct agaatgttca ttttcaacgc
     3601 ctaaacgctc aatcggtaca ccaaacgcct tggcaatttg ttttgtgttc caatcattgg
     3661 aattgataag cttcaaaaca tcggtgttta tttctaatgt tttgtagtcc attgtttcat
     3721 caagaatgat cgttctaaga gcattatctc cactacttga accgttggct tcttcaaact
     3781 tttcacgaat tgcgttcttt gctgatccgt ctaaatctga cttatgaacc ttcaaaatac
     3841 ctgacccact gacaccacga gtaaagaaat tgtgcaatgt gcggttgcca gcttgttgaa
     3901 ttttcagttc atcacgtaac gcataaagtg gtggtaatcc tgttaaacca tcttgggtga
     3961 aatatttgaa gtgcaaaata tcgctagact tcacacgtct ttttttatca ccaatctgat
     4021 aagacaacgt accattatct aattgagtaa ccgttacact tgagttaggt aataagtgaa
     4081 tttcttctac cttttcaccg ttgcgtttaa tctctgcaaa gctattaccg tttagcaaca
     4141 tattgaccgc caaagcaaac tttaaatgcc aaccatccat ttctgaatta ggtttctcgt
     4201 ttagtaactt caccagttca ttatcagctt gtggcatatt gtttttgacc aattgaatcg
     4261 gacttgaagc aatatcacta gcaataattc gaacggctgc aaacacatca ctatttctta
     4321 atgcacgaac gcttgtaaag ctggtggtgt aatcatccga ttggattgat accacatgtt
     4381 gtaggaaagg atctcccgtt gtattttgtc tcgctttaaa aaatgccatc ttttaacctc
     4441 ccttctgttc tgtatttagc atgaccgcta ccccaataag cactatgccc agcgctaaca
     4501 agccatagta gacatttgtt agtaaggtga tagcaaccaa cacacacacc aatccaagaa
     4561 gtaaaagaac ggtatgaatg taattagaaa gaaaattctt cacttgtata aaattcattg
     4621 tctgctttcg ctccttccgt tgtggtgaag taatccattg caaacacata ggcattgatt
     4681 aatgcagcta ttggatcaat tttgttactg ttctttgatt tattaatctg aatgccgttg
     4741 ttatccgttt ttaaaatagc gttgttcact gcatgggtga gaatcgtatt tttattgtga
     4801 ataacgttgc cttcataaac ttgttctcta aaagttcggg tcggaacgtt tagagtaatc
     4861 gttccttgtc ttacttctaa cattgggtag ttggcttttt cagctttact gataagcgaa
     4921 ttagcgttat acgggtcata acaaatcccc atacattcca aatcattttc ttgaatcaaa
     4981 tcttgaataa actgaaatac ttggtcatag tccacgattc cactttctaa ttgagtaatg
     5041 gaacactcac cagctttttc tagttctcga taaggcaaac cgtcgcgctt ttctttgtct
     5101 tgaagcccat atttggtggc tacaaagcta tgtgaatcac aataaagttc gccgttatct
     5161 agcggtacga tccatgaaac gctagttaaa tcatctgttt tagataaatc cactccgata
     5221 taaacgggct tgcctgtaat atcttgtggt tctacttcgg ttgcctgcca gtcttcagca
     5281 atcatatagc tgtcctcact cgcttgtctc cataaattga agtttttcac caatacagaa
     5341 ttaaggttat tttgtttcaa agcaaggttc acatcatctt gaatggttgg aatcattact
     5401 tttttgattt cttcactttc aaaaatcgga ttggctttga tccatttctc ttgatcgtga
     5461 atttcttcgg gatcgtccaa ttcccatatc gctataaagt acctgtcggc ttgttcttcg
     5521 ccttttagca cacgatctaa caataagtat tcctcgtaca taggaacgtt taaatcaagc
     5581 ccagcggtac taataacaca caataagccg ttcttttgtt gggtcattcc tgacttaatg
     5641 acgttgtacg ttttacgagt ttttgcttcg tgccactcgt caagaattgc ggttgttggt
     5701 gcaaacccat ctagcgtgct ggtttcacta gccagtgcca tagcgaacga attagagggc
     5761 aagtgctgaa tcttagagtt cataattttc agttgtggtc ttaaaaactt actagacttg
     5821 accacgtttc taagtgaatt agacagcata tcatagccca atttagcttg tttcaaagcg
     5881 ttacttacaa acaatacttg ccttgcttct gctggttctt gttccataat caacgcattg
     5941 gcagccatgc cacttgcgat atacgttttc ccattcttac ggctcatact ggtaaacgct
     6001 cgattgaatc gcctgtaatt cccttcttta gtacgccagc cataaagact gcctagaatg
     6061 aatttttgaa atcctaacat cttgattgct ttaccgtccg tagatggtag caactcaatg
     6121 aatttaatcg ctttggtggc ttgttcttca tcgaacacat aagggaagct atctgactta
     6181 gaacgctcca aatcgcttat atgacgctca cacgcttgga taattttctt gggtgcaatc
     6241 agcttacctg ataggacttt ctcaatgtaa tcattcatga attcatcaac tctttgaacg
     6301 ggtcttcggg ttcatcattt ttaaccttgt tcaaagctag ttttgatcgt gattctaagg
     6361 tcatacctag tgaggtggct tgttttttca aatcactcat agcttgtgat tgaacccgaa
     6421 ccgctggatt ggctttcttt actccgtttt cgcatgtgat aaacgtacct gattttctta
     6481 tctcggcttc ggctgttttc attcgagcaa aggctaaaca ataggctacc aatgaaccat
     6541 aatctgcttc actcaatgga taatcttttt ttaatgttgg tacgatacga ttccattcac
     6601 ttctagcgct cgctgccatc cagtcagggg gcgtgatatt tattaatggt tgctgcttga
     6661 acaattcttc tctagcgtcc acacggattg ctttttcttc acttgatata ttgccttttg
     6721 tatcgtgtaa tattttaggg tgtcccatgt tgtcctcctt tcagacaaaa gaaaaaggaa
     6781 cgtaagcaat taagctcaca ttccttcgtt tttcgatcag aatttttctt tatctaattt
     6841 atctatataa atgatttttc cgttctgcat ttgaatggtc acttgaccaa aatgaggtaa
     6901 ctcaatggtg cgctgtttcc ctgttttatc agtaataaca attgtatttt tcaataaatc
     6961 tcctactttc attatatact ttttcacttg tgattacaag tgtaatcgtt atttgtaatt
     7021 acccttatgc cttttcacaa cggttatttg taccaaaatg agtaggcatc gattgtcagt
     7081 aagtttcaca ctatgccccc ttaactcaaa gggtactctt ttctcgtctt tgtctttcat
     7141 tggctgtctt ggaattatgg tgtgcatggc ataaagaacg aaaattagtt tcatctaacc
     7201 gtctattcca atcatctttt agttcaattt tgtgatctac aacatcagct ttccttatca
     7261 agccttcttc taggcaatcc tcacatactg gatgatttaa ccgatacaag tatgaagcct
     7321 ttcgccatgt tctactttga tagaattgaa aatatttacc ttcgctttgc tttctttctt
     7381 cacgcttaat tgccgtttgc tttgctttat gcttcccaca atacttctgc ctataatcaa
     7441 tcaatgtttt acatccagca tgattacatt gttttttaac tgccatagat taccaaccaa
     7501 agcgatctcg ttcgtcagaa tcatcttgtt cccattttga tttgtattca atataaggcg
     7561 aaactatctt caagcacgtt ccgtctttaa aattaacttt agtgatttta tcttcctcaa
     7621 aactaataaa gtctacatct tcttgagtgt ttgaatactc aatgtgtttc tcaccatcga
     7681 agtatctaat actttccata tgcataatta ttctacctcc aataaattag ttatcaattc
     7741 atcatctgtt agtagcaaag ccaagaactt atcatggatg tttaaaaaca aattatcctc
     7801 attcgatgta agctctcgtt gtctcgctcg atcatatagg ttaagcagac aattaaattc
     7861 atccattagc tcacctgtat catcatagtt gccactatct accaatgatc ccactactag
     7921 cgattgacac cacgctactg actcctgcag ccaatcaggt tcttcttcca ttatctttaa
     7981 tacttctgga tcaaatgtgg aataatcttc tgttaccaat cccacactaa atgccagcgt
     8041 gttcattaga aaccgatcca tttcatctgt atattgaaag ttcggtgctt tgtttgttga
     8101 aataatattg agcatttact ttcctccttg attcgtaaca cgtggtaaca cctacgtaac
     8161 aggtacccgt taccactcaa tcccaacggg gacaagggtt ctagcgtttt ggtaacaggt
     8221 aacgggtaaa atccagtaac attattcccc agcatattag ctaactattt caaaagtttt
     8281 aaggaaataa ggtgttaccc cgttaccaaa ttgctgtatc ccttggggca gtaagggtaa
     8341 actagtaacg ggctaggtgt tactgacccg ttactacccg ttactttttg tactttccag
     8401 ctcgattttt attttcatag gcatttgact tattgtaaaa tcctcgtcgt ttatttcttt
     8461 ttggcagttg cttccgttcc caaccaggaa tactgctaat aatcaaggca atctttttag
     8521 tttctgcatc tgatcgacca ttcaataaat cctttgcttg tttatcaaaa acagcttcca
     8581 aaatatcagc ggtgagtacg ctatcaacta aataaaactc ttgttgcata gtagctccat
     8641 tatagaaatg ctgtaaattt ttctcattac atttactgtc aggatagttc tgataataag
     8701 aacgtctcgc ccacgctgga gcttcttccc atttatacgg gatttccatt tccaaatatt
     8761 tagtgattgc ttctttaatt ggattctcca tcttggcatc ctcttgatag tcttttgcaa
     8821 tttctaagac ttctttatct tcatcgggac taaaatagat tcgttgtcct ttatcaaaaa
     8881 gcacctttgc ttcagctaac acttgaagaa aatattcatc ctttgtttta aacggattca
     8941 tctttggctt attcttacaa ggcaaaggat aaaaacggcg gtttcctgta tcatctttca
     9001 aatactgccc atcattcgac gttccaataa atacacattg ccttgcccaa gctatgacat
     9061 tccgaccata aggaggtcta attttatcga tcttggcact gatgaagttt tttatctgtt
     9121 caattttagt tttgttcatt gaagataatt ctccaagttc aataattaca ttgccttgaa
     9181 gctgcatata atcatctttc ttttcaccta gcgaatctaa tgaatcggtg aaaaaatcat
     9241 ccgtgtaaag taatgctgga acggtactct taccaatccc ttgcggtcca tccaaaactg
     9301 gaacgatctc aaactttatt cctggtttat agatacgtgc gactgatccg actatccaac
     9361 gttttgttac ttctcgtgtg taaaggttgt cgtctacccc taaaaggtca ataaacagtg
     9421 tttccagtct aggctgttga tcccatttaa ctttttcgat acgttctttt actgggttga
     9481 aaacattctt tctagccaca tttactactg ctttgtgtat tttgttgtca tcaaaaacta
     9541 cattgtagtt ttcttcaata tatgccgtta aggcatcctc aaaactgtct tcccattctc
     9601 cagcggtacg attgatccaa ggagaatctt ttaataggtg aatgtatccg ctaaattcgt
     9661 tataagcaat tgttccagct aaattgctat cattggttaa aatcaattct gcattcacaa
     9721 cactgttact tttaatctga tcgtctttat tgcatcttaa tcgtgacatc caggttttca
     9781 attcaatgat attgtcttca cgctcgctgg cttcgggaaa gtctaaaata ctatctaatt
     9841 tatgcttatg agccatgagc tttccccctc aaatcttttt taatcatcga tcgaataatt
     9901 tgattcactt ctttatcttt tagcggtggt tcattgtacg catttgctgt ataaatcaat
     9961 tctgctaaag tttgattatc tacaccgtgc catagtaacc aaccgcataa cttagttaaa
    10021 tgatcgtttc tactaccttc gccagcgcct tgaacaatca tatctaattt ttttgctgtc
    10081 cctgtttttg ctacgggtcg gtattgagtc tttatttctt gcggtttttt tagcagtagg
    10141 ttcactagcc aatcgggagc tggtgcaata ttatttagag atacgttttc aataggttca
    10201 tatgctctcc caacacttct tgaaataatc atagttggcg ctattggaac tcctttagtg
    10261 ataatatcaa tcccgcttaa acttttacct ttatcatcac gcccgaagat atcagcaaat
    10321 gcacctgttt cttgttggat aattacacca tcaggaattt taaagaagaa atggattcct
    10381 ttacctggtg tgatttccgt ataagtagaa ggaaatggat catacattga agcaagtttt
    10441 ttataattct caacgccgtt tcgtgtttca gaatgttgat ctaggtcaac taccaccatt
    10501 tgatgatctt caagcattaa cccaatattt ctagttgcat cttccgtcca ccattcatta
    10561 atttgattag aatcgttagt tgcatcattc caaccactag agccttttat tggaatttta
    10621 gttccaggag ataagggata aattgaccat cccgactttt gtaattccgt tgctgcttga
    10681 taattatttg ccaataaacc tctcctcctt tttcaaaata ctggtataaa tcattcagac
    10741 acctccatga gcttcaactg ctcaagttcg tatttcaacc catcccgaac aaattcagca
    10801 ccttttaatt gattctgata ctttacccat gtattaaatg ttttcaccct tgcttcttgc
    10861 atatcagtaa tgtttaacca tgcggttaaa atctgatttt ttagctcgtt gatttctcga
    10921 caaattattt tgtgttctgc atgttttttg agtacataca aaactgttag tttttcattg
    10981 aatgaatttc caaagtaggt tttcccgata ttattttcta gttcagcgat cccttgatta
    11041 atttcctcta attcaaggct atcgagttgg tttaatagtt ttgttttgta catctactta
    11101 tgccctccag ttcttaattg atagtcgtac tcgtcataag cagtgaagag atataacgct
    11161 aatcctgcaa agatagctat ttttgcaatt aatggtaata agccgaaaat tccgccgatt
    11221 aaaatgcttc ctataattaa gtttcgtttc ataatggttc cctccgattt tttaatatgg
    11281 tataatcgga acaaagaata tcgggtttta tcccaattaa gttgccttta tcctgtccgc
    11341 caagactgtt atggataagg ctttttcttt gttctcgatc atgttaatgc tcctaagttg
    11401 tctgcaattc agcagaacga ttataaattt tctcactcac tgaaaactgc aacccatatt
    11461 tttctttaac agtcattaat tctactgtct catctaaaat aggttcacga tcaaccaaca
    11521 tttcaggagt catatctttc ttttttacca ttttggaatg accataaata gttgatacag
    11581 ctttattggc tatcacattc gctttaatgt agtctactgg ttttggttca gtaatagcgt
    11641 ggcttaattt agtcattgct tctttttggt gttctttgtc tagcatgcgg aatacttgaa
    11701 agccttcaag tcctgtggac tggcgtagtt ctttgatgat gtcgaaaatc cagtcttgaa
    11761 atgcctcggc ttctttttta cggcttttga atgccatgcg gtaaatattt tttttcgttg
    11821 atgatgttta catcttgtgt tctgcccaat gaatcgatga ccttactagt agtaacccca
    11881 tctttaggca aagagtgaat ggctctagta acctgtttaa gacctaacgc ttccgcaaca
    11941 tctttcgcca ctgcccacca ttcatcatta atatcgacaa atcttataat gtgtccgttc
    12001 caaatttcag ttttcattca acgtcctcca gttcaagccc tagcaagctc gctaacaact
    12061 caacttgctg atacaaaatt tcttgatact cttcaataga aagtcgttct ttaaactctg
    12121 cttgtactaa atcgttcaag ccacgcaata ctccgcttac cgttggttct tgtggtagca
    12181 atttttgata gtccatttgt tcgtacaaac attcaaacgt ttcttctagc gctttgcctt
    12241 ccaagtttgc taattctgcc gctgtgtctt ctgtaagaat tgttgataat actttttcga
    12301 ttgctttatt ttgtgtcgat tccattatta tttcctccta ttaatccctt tgcccccacg
    12361 gcttgcgttt atttttggta ttcttccata aagcgatcac attcatcttg tgaaattctt
    12421 acgactccat caactataat caactttaaa ccgttaggaa catatttctg catcatagta
    12481 ttgtatgaaa cgttcatata cttagcagct tgtgccttgt tcatatattt tggaaactgg
    12541 tacttttttt ctaataatga aagtaacgtt ccttgagttt gctctacttg ttgaacaact
    12601 cgatcgtcaa taattttaaa agctggatca tcaagcgtga gtttgtcaat ttttaattcc
    12661 atatttatcc cccctcgtta gcatacattt ctatacaaag attttaaaaa aatgcaagag
    12721 gctcttgaat acatttctat acctcttata aactcaatat accatcatac cttttcattt
    12781 gcaagaattt taacatactt ttttgaactt ttatgtttca cacatattta aatcgtgata
    12841 tagtagagaa aatatatgaa tggtggtgaa aatgtggcta gaaaaaaata ccccgaagca
    12901 gtggagacag gacaaagaat tagactggct agaattgaaa agggactctc aatggaacaa
    12961 cttggaggta aattatcccc accggccagt aaaggtgccg ttagtaattg ggaaaatggc
    13021 tataatcttc ccaataacac caggcttaaa caactttcaa aggtattaga tgtatccact
    13081 acctatctat taaacggatc ttatacatta agagatatgc atatgatgcc cgaaattaag
    13141 cgaaatgtgc ttgaaaagca attgaaaaaa actattgaaa aaaataactc tataattgat
    13201 tctaatcttt ctaatgctat caataacatc gaagaagata acctgactgt ctcacagaag
    13261 atttatttaa caaacgcaat aaaatatctt aaaacaggat ctcgaattga atttcctgac
    13321 gggagtaccc caattgctta tctcgcagct cttctaaata cgcttttacg atatgactca
    13381 ttaattaatg attcagaaaa aaatacagat gagaaaaaag agtatcaaga attttatgaa
    13441 aatgatcttc taaaagagtt cacagctctt gttactgagc taaataaaca ctattcaaaa
    13501 taataataaa ctgcctcctc caaccgcccc cacggcttgc tacactatgg agggaaaact
    13561 attatggcaa cattcaagca atatgaaact aaaaaaggta aattttggct ctatgaagcc
    13621 tatcttggaa tgaacaaaat gactggcaaa cctgataagg ttcgtagacg aggttacaaa
    13681 acaaaaaaag aagcccagct tgccttgtca cgcttgcaag tggactacga taaaaacggt
    13741 attaaaaaag cgaacaacga aacgtttaaa gaagtttatg acttgtggct agaaacatat
    13801 aaaaccactg tccgagaagc caccttcatg aaaactgaaa tcaagtttag aaagtggatt
    13861 cttccaaaat atggaaatct tcgtgtgaat gaagtcacgg taaaaaaagc ccaacaaatt
    13921 gtaaatagct gggctgaaac tactgaccaa tacaaggtac ttcattcaac ttctaagcgt
    13981 atttttagct acgctatcaa tcttgggatt attgagacta atccactaga caacgtatta
    14041 atgcctaaaa gaaccaccac taagcataaa gaaaaggtca aagtttacag taaagaacaa
    14101 ctgatcacct tgtttgatta cctaaacagc aaacctgaaa cttatcgaaa cgactttgat
    14161 aaaacgttac tacgctttct cttttatagc ggggttagga tcagtgaagc ccttgcattg
    14221 aattggtcag acattgattt tcaagaaaat acggtcacca tcaataaaac actaagccaa
    14281 tccaaaaatg gctataaaat cagtgatcct aaaactgatt gtagtgctgg ggttcttcca
    14341 ctagatgaaa gaacaatttc tatattaaaa aaatggcaga ttaatcagcg gaaatatatg
    14401 ctgacacttg gtgtaactga tccgactatg attttttgtg gtatctataa agaactaatc
    14461 acacaccacg ctatttatgc aagattgatt actatttgtg aaaaagctat ggttccattt
    14521 ctcggtgtgc atgtagcaag acacactcat gcttcaatgc tcttggacgc tggagctact
    14581 atggtagaag ttcaaactag attacggcat acaaaaatta gtcagacgat cgattgttac
    14641 ggtcacttgg caaaagaaac gaaagaaaag actgttgaaa aattagtaca acacttaaat
    14701 attgactaga tggcatacaa tatggcatac agtaaaaaat aaaaaaagga gaaaccctat
    14761 catatcaagg tttctcctag ctttatagtc tattcttctt cttccgccat gaatgtattg
    14821 aatacttctt caatcatgtc ccattctgcg tctgtttcga tttgttctaa ctcgccttct
    14881 gttccatctt cgttttcgat gtatgcataa gcttgtaatt ctacatcttc atcttctgaa
    14941 acaccagcag gatataaaag aacatagtta cgtccaaatt cttcttgtcc gtcaactgtt
    15001 agtaggattt catataatgt ttcatttcct tggtcatcca ccaatgtgat gtgttcgtgt
    15061 ccttcatggt cgtgggaatg gttatgttct gtcataagat tacctctttt cttttattat
    15121 taaaataatt gcttacctga gccgtctaaa taattttgta agatcatcac agctgctaat
    15181 ttatcgatga ctttttttcg tttggctctt gaagtatttg cctgctctac tagcatacgt
    15241 tctgcctgaa cagtggttaa tcgttcatct tgataatcaa ctggcaagga gaatagttct
    15301 ttgatctttt ctccgtatga catggaagct tctgcccttg gtccgatgga attgttcata
    15361 tttttaggta atccaactac aaaccggtct acttcatatt gtgctactaa ttccttcaac
    15421 cgatcaaagc caaactcttc ttctgcttca ttgatccgaa tgatctctac tccttgagct
    15481 gtccagccaa aggggtcact gatagcaaca cctactgtct tagaaccgac atccaagccc
    15541 attactctca taaattcact ccatgatttg agaggtagtc cttgacaatt acttccatga
    15601 tctcatcgcg ttcgtgacgg cgaatcaaat ttcgagcatc acgataacga ggaatgtagg
    15661 ccgggtctcc agaaagcaag tagccgacaa tctgattgat tggattgtag cctttttctt
    15721 ccaaagcccg ataaacaatc gctaatgttt cgctcacttc ttttttgcga ctatcgtcaa
    15781 aaccaaaacg tacggtttca tctgtaaaac ccatgattct acacctcttt cccgcaaggt
    15841 aattccagct taccttctac aaccttattt tactcgaaga aagggaaaac tacaaacaaa
    15901 tattccaaac ttccgaataa attaagaaac actatttgta tgtttttttc cttttttctg
    15961 tagctttatc aaa
//
LOCUS       DAZULY010000043        15955 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000043 DAZULY010000000
VERSION     DAZULY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.043"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(390..812)
                     /locus_tag="ACG81A_000917"
     CDS             complement(390..812)
                     /locus_tag="ACG81A_000917"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFS5014306.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(826..2232)
                     /gene="atpD"
                     /locus_tag="ACG81A_000918"
     CDS             complement(826..2232)
                     /gene="atpD"
                     /locus_tag="ACG81A_000918"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFS5014307.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2255..3157)
                     /locus_tag="ACG81A_000919"
     CDS             complement(2255..3157)
                     /locus_tag="ACG81A_000919"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFS5014308.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3171..4727)
                     /gene="atpA"
                     /locus_tag="ACG81A_000920"
     CDS             complement(3171..4727)
                     /gene="atpA"
                     /locus_tag="ACG81A_000920"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFS5014309.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4750..5292)
                     /locus_tag="ACG81A_000921"
     CDS             complement(4750..5292)
                     /locus_tag="ACG81A_000921"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFS5014310.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5279..5803)
                     /gene="atpF"
                     /locus_tag="ACG81A_000922"
     CDS             complement(5279..5803)
                     /gene="atpF"
                     /locus_tag="ACG81A_000922"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFS5014311.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5894..6109)
                     /gene="atpE"
                     /locus_tag="ACG81A_000923"
     CDS             complement(5894..6109)
                     /gene="atpE"
                     /locus_tag="ACG81A_000923"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFS5014312.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6162..6881)
                     /gene="atpB"
                     /locus_tag="ACG81A_000924"
     CDS             complement(6162..6881)
                     /gene="atpB"
                     /locus_tag="ACG81A_000924"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFS5014313.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7183..7647)
                     /gene="smpB"
                     /locus_tag="ACG81A_000925"
     CDS             complement(7183..7647)
                     /gene="smpB"
                     /locus_tag="ACG81A_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFS5014314.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            complement(7669..10026)
                     /gene="rnr"
                     /locus_tag="ACG81A_000926"
     CDS             complement(7669..10026)
                     /gene="rnr"
                     /locus_tag="ACG81A_000926"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFS5014315.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            complement(10029..10784)
                     /locus_tag="ACG81A_000927"
     CDS             complement(10029..10784)
                     /locus_tag="ACG81A_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5014316.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10887..11123)
                     /gene="secG"
                     /locus_tag="ACG81A_000928"
     CDS             complement(10887..11123)
                     /gene="secG"
                     /locus_tag="ACG81A_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFS5014317.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11241..11519)
                     /locus_tag="ACG81A_000929"
     CDS             complement(11241..11519)
                     /locus_tag="ACG81A_000929"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5014318.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11632..12600)
                     /locus_tag="ACG81A_000930"
     CDS             complement(11632..12600)
                     /locus_tag="ACG81A_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5014319.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12815..12859
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12883..13857
                     /locus_tag="ACG81A_000931"
     CDS             12883..13857
                     /locus_tag="ACG81A_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFS5014320.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13862..14437
                     /locus_tag="ACG81A_000932"
     CDS             13862..14437
                     /locus_tag="ACG81A_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFS5014321.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14504..15643)
                     /locus_tag="ACG81A_000933"
     CDS             complement(14504..15643)
                     /locus_tag="ACG81A_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFS5014322.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4359 a         3443 c         2856 g         5297 t
ORIGIN      
        1 aatctgagcc agaatgtggt tcattctgac tcagatttga ttttgcacaa ctcatcagtc
       61 cttattgaca aagagcccaa aagtcttgtc gcagtccctt attccaaata aacggtgttc
      121 aatcatctga ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttccat
      181 ttgtttctct aatactcagg acaaaacgct gatttcacaa cctccgatat acggtgttcc
      241 agaagtagct tcttcgaaaa ataagctgag atttcacaaa aatttgagaa gcaattttcg
      301 taaattcctt cttatgtctt gaagctgacc acttctgtca taacctcttt ttactgttct
      361 taaacacgga taaacatccg catctcattt tatgagtgct tagatacttt gatacgattg
      421 attgctcggt gcaacgcaac agttgcccgt ttcaattcgt tcgtatcttg ttttgctttt
      481 gcttcttcga tttggcgttc tgcgcgttgt tttgcacgtt ccgcacgaga tacatcgata
      541 tcacgttcac gttccgcact atcagcaacg attgaaacaa cattatcacg aacttccatg
      601 attccgccat taacagcgat ccaatcgacg tgggtatctg aatctgttcg ttttacacga
      661 acttcatcaa tcgtcaatgg aacgatgatt ggtgcatgtt taggcaaaat cccaagctca
      721 ccagctgtcg tacgtgcaac gacgatagct gcatggtgat catagaccaa accggcagga
      781 gttaccacat ttactgttaa atattgatcc atggggcacc cctttctagt agcctagtgt
      841 tttcgctttt tctacgacgt cttcgattcg gccaacactt cggaaagctt cttcaggaag
      901 atcatcgtat ttgccttcca agatttcgcg gaatcctttc actgtttctt ctacagggac
      961 atatgaacct ggaagaccag taaattgttc agccacgtta aagttttgtg acaagaagaa
     1021 ttggactctg cgggcacgag aaactaacac tttttcttga tcggataatt catccatacc
     1081 taagatagca atgatatctt gtaattcacg atatcgttgc aatacgtgtt ggacttctgt
     1141 tgctacttta taatgttctt ctcccacgat ttcaggtgct aaagcacttg aagaagaagc
     1201 caatggatct accgctggat aaatcccttg ttctgttaat ttacgttcca agttggttgt
     1261 cgcatccaaa tgggcgaatg ctgttgccgg cgccgggtca gtgtagtcat cggctggcac
     1321 atagattgct tggatcgatg tgatcgaacc ttttttcgta gatgtgatac gttcttgcaa
     1381 ttgacccatt tctgttgcca atgttggttg ataaccaacc gcagaaggca tccgtcctaa
     1441 caaggctgat acttcagatc cagcttgagt gaaacggaaa atgttgtcga taaacaacag
     1501 tacatcttga ccttctacat cacggaaata ttccgcaatc gtcaaaccag tcaaagcaac
     1561 acgcatacgt gcacctggtg gttcgttcat ttgtccgaac accatggctg ttttttcgat
     1621 aactcctgaa tctttcattt cataatacaa gtcattacct tcacgagtac gttcacctac
     1681 accggtaaat acagaaatac caccatgttc ttgggcgata ttatggatca gttcttggat
     1741 cagtacggtt ttaccaacac cggcaccacc gaatagtccg actttcccac cttttaaata
     1801 tggggcaagc aaatcgataa ctttgatccc tgtttccaaa atttctgtac ttgtacttaa
     1861 ttcgtcaaag gctggtgctt ttttatgaat ttcgcttctt tccgcatctt ctgggaaagg
     1921 tgtttctaaa tcgatcgtat ctcctagtac gttgaatacg cgacctaatg tttctttacc
     1981 tacaggaaca gagattgctt tgcctgtatc aatgacttcc attcctcttt gtaaaccatc
     2041 tgttgattcc atcgcaatgg tccggatcac tccgtcccct aattctaacg ctgcttcaag
     2101 aacaactttt gatttatttt cgtcattttt ataaacaact aatgcgttgt tgatatctgg
     2161 taaggattgg tctaaagaaa attccacgtc gacaacggga ccgattactt gaacaatctt
     2221 gcctgaactc atcttttttc ctccaatctc aatactattc tagtgccgaa gctccggcaa
     2281 caatttcggt aatttcttgg gtaatagccc cttgtcttgc acggttgtaa gaaaccgtca
     2341 gctcatcaat gatattagca gcattatcag ttgctgtttt cattgctgtc ataccagcag
     2401 catgttctgc tgttttggcg tcaacgatcg caccgtagat caaactttca gcatactgag
     2461 gcaataactg agacaagatt tcttcttttg aaggttcaaa aatatattct tgttcgaaag
     2521 tagttgcttc ttctggatct aagtctgaga ttggcagcat tttttctaca cgaaactgac
     2581 tagttagcga attgatatga tggttataac atacgtataa ttcatcgaat acttcgtttt
     2641 ggtacatagt tgttgccata tttacgattt tgcggacttc atcaaagctt ggctgatcag
     2701 atagattgcg cagctcatat gctagattga taccgcgtgc tttaaagaag tctgcaccag
     2761 ttccgccaat tgcgatcatc acgtaatcat cgggtgattg gtggtcttct tctaaaatag
     2821 acatcgtttg tttcaatatc gagctgttat atccaccgac taaaccgcca tctgcagtga
     2881 tgacgatata acccgttttc ttaacaggac ggctgagcaa catgctgttg taattgatat
     2941 cccctctagg attttctgag gcaatatcat ttaactgagt agcagtcaaa tgcgtaacga
     3001 tttctcttac ttttgttgca taaacctgaa acttctgtga agaagcttca gattttgtca
     3061 acttagaagc agacaccatc tgcattgccc gcgtgatttg gctcgttttt ttcgttgaag
     3121 cgatacgtgt cttgatttca tttaatgaag cacccattca tctcacctca ctatgcattt
     3181 tgaatggatt tcaatttatc ttctgtagag gaattatttc cttttgtagc taagaacatg
     3241 tctttatatt cctgaatcgc actgttcaac gcttcttctt ctggaagatc ttttgttgta
     3301 cggattgttt caaagatatc tgaatgattg gtatccagat attcgaataa ttcatgttcg
     3361 aagtctaaaa tactgtctac agggatacta tctaagaatc catgtgtcaa agcatacaaa
     3421 atcactactt gtttttcaac agctagcggt gcatgcaatt tttgtttcaa gatttctact
     3481 gtacggcgtc cacgatttag ttttgcttgt gttgctgcat ccaagtcaga accgaattgt
     3541 gtaaatgctt ctaattcacg gtaacttgct aagtctagac gtaaagtccc agcaactttt
     3601 ttcattgctt tgatttgcgc agaaccccct acacgagata cagatagtcc ggcatcaacc
     3661 gctggacgag tacctgcgta gaacaagtcg ctttctaaga agatttgtcc atcagtgatc
     3721 gaaataacgt ttgttgggat ataagcagag atatctccag cttgtgtttc aacaaatggt
     3781 aaggcagtca ttgaaccgcc acctaaatca tcacttaatt ttgctgcacg ttccaataag
     3841 cgtgagtgta agtagaaaac atcccctgga taagcttcac gacctggtgg acggcgaagc
     3901 agcaaggaaa gttcacgata agcaacagct tgttttgaaa gatcatcaaa aatgatcaag
     3961 acatgtttcc cgttgtacat gaactcttca cccattgcag taccagcata tggtgcgata
     4021 taaagcaacg gcgctggttg agacgcaccg gcattcacaa cgattgtgta atccattgcg
     4081 ccatattttt tcaatgtttc aacttgtgta cgaactgtag aatctttttg tccgattgct
     4141 acatagatac agatcatatc ttgacctttt tggttgatga tcgtatcgat cgcaatggaa
     4201 gttttccctg ttttacggtc accgataact aattcacgtt ggccgcgtcc gattggtaca
     4261 agagcgtcga ttgcttttag acctgtttgc attggttcgt taacagattt acgttgcata
     4321 acgcctggtg ccattgcttc tactggacga gctttgtcag taacgatttc acctagtccg
     4381 tcaattggct gacctagcgg attaactacc cgtccaatca aggcctctcc aactggaact
     4441 tccatgatct ttcctgttcg ttttacttta tctccttcac gaatggactc gaaatcgcca
     4501 aggataatga tacctacatc atttgattct aagttttgcg ccattccgta tgagccattt
     4561 gaaaattcaa gcaactcacc actcatcgcg ttttctaaac catgtgcacg agcgatcccg
     4621 tcacctacat aagttacggt accgatttct tcgactgaaa gcacgttttg ataattttca
     4681 atttgttctt taatcaaggc actaatttct tctgctttga tggccattcg attcacctac
     4741 ctctttgtac tatctattta attgattacg catattttct aattgcgtgc gaatgcttcc
     4801 gtcgattact cgatggttag cttcaacaat cgcgccacca ataatagaag aatcaacgat
     4861 ttgttttaat tcgactgatt gatagttcat tgtttttgca acattctttt ctaattgtat
     4921 tcgttgttcg tctgaaagtg gaactgctgt tgtgacactt cccaacagca aaccttgatg
     4981 ttcatcatat cgacgttcat attcttcgat catgaacaaa agatcatcca ttcggttata
     5041 cgcataaaca acttctaaaa agtttttaac gattccatca tagccactaa caagcttgtc
     5101 catgatttct cgtttttcat gaggttctag acgaacatca cttaacatat tacctaatcc
     5161 aggtacttgt tcatagattt gacgtaagct tacgagatct tgataaattt cttctgcttt
     5221 atttgaatcg atcgccagtt caaataaagc tttcccgtaa cgtcttccta ctgtatattt
     5281 atctagtttc atttgaagaa cctagacctt cgatatattg attgattaat gattcgtgca
     5341 tttctggaga taattctttg ttcaagattt tttccgcgat ttgaagagat agttctgcaa
     5401 catcgtcttt tacagagttc agcgctgaat cacgttctac agtgatatca gcttgggctt
     5461 tactttttag acgagctgct tcttcttgag catccttcaa aatattttgg cggcttaatt
     5521 ctccactttc tttcgcattt ttgatgatat cagctgcatc agaacgagag gctaacaatt
     5581 gttgttcgcg ttcttgttcc attttcgctg agttgatgcg agattgttct gcagaatcca
     5641 aatcattggc gatcttgtct tcacgttttt tcaaaatatc gctgattggt ccccaagcaa
     5701 aatgcttcaa gagagccagt agtatcaaaa atgagccgct gacaacgatg acatttccta
     5761 acatcgcatt gccaacttct gcaattgcca attgattcag cataggcgag ccttcctttc
     5821 tttgaagtac ttctagtttt catgtaaaaa agcagtaaca aaccaaattg tttctgcctt
     5881 aatactgatt caattaaaca ccaaaaacta gaatcaaggc gacaactacc cctaagatag
     5941 gaacagcttc gactagagct accccgataa acattgttgt acggaattga ccagacattt
     6001 caggttggcg agccattgat tcgattgttt tagagataac ttgaccgtta ccataaccgg
     6061 ctccgattgc tgctcccatg attgcgattg ctgctgcgat ataattcata attgttttcc
     6121 tcctaaatta gataaatagt agtttgtttt taaaacgaat attattcttc cacttcaatc
     6181 ttatggctca tataaaccat agataaagta acgaagacaa atgcttggat gcttccgata
     6241 aacaaggaga atgcgatcca gaccatctct aatggaattg ctaacggcaa tgagaaccag
     6301 cctaagctta ttaccatttt tgcaatcaag gttagcaaaa cttcccccgc ataaatattc
     6361 ccataaagac gtaaagcaag tgttaataag tttgtaaatt cttcaatcag tttcatcgga
     6421 aacataaaac tatatggttt caagaacgaa tttacgaaat atcctttgaa gcctaacttt
     6481 ttcaccccaa ataagtgagt aagtaagatc attataaatg ccaaagttaa agtaacaaac
     6541 gggtctgctg tcgggctttt ccaaagtgtt gtttcgttag gtaaaacgat ttttgttact
     6601 aaaccgatgt tgtttgcaac aaaaacaaat aaaaacaatg taaatgccaa caaatggaaa
     6661 ttgctcaatt cttttctagg cagattatct gttacgattc ctcgactaaa atcaatgacc
     6721 cattctatcg cattttgttt gcctgttggt ttcaacttaa gatttcttgt aaaaaagtaa
     6781 gctaagaaga aaacaatcaa acaggtcagt aacaccatta tacaaactgt accatcaaac
     6841 caaattggtc caatgtggaa cgttagcgag cgttcatcca atccatttca cctcttttcc
     6901 ccgaaattca cataaaatca attcatgtgc gtatctggtt ttttccaatc ggtttctaac
     6961 cacacggtat cataccacca tgaaacagaa aattcaaaac attttcatga aaattagaca
     7021 cttttttatt tcagtcaaaa acgaagcgaa tttttcttta tacacggtgc tcctgatcta
     7081 agaaaaaacc tatgataatc atgttttatc agattaaatg ctaacaaatt ttatcattaa
     7141 gtaaaagcaa aagcaccgaa tatgcgaaaa aggataatga aattatctag agaaattttt
     7201 taatgttcga tcaatttgtc gatcgatatc ttttcgtttc aaatcttccc gtttatcata
     7261 agattttttc cctttcgcca gaccaatcaa taccttggca tagccgttac gaatatagac
     7321 ttttaaagga acaacagtga ttccagtatt tttcaattca ttttcaagcc ggatgatttg
     7381 ttttttgtgt aataataact ttctcgtgcg caacggatca tgattaaaaa tatttccttg
     7441 ttcataagga ctgatatgaa cattatgcaa gaaagcttct ccgttgcgga cgcgaataaa
     7501 tccatctttt agattgatcc ggctgtttcg tatcgacttg atctcggttc cttgcaagac
     7561 catccctgct tccatcgtgt cgatgatcga ataatcatgg cgagctttct tgttttgtgc
     7621 aattaatttt ccctcgcctt ttggcatagc tgtttcctcc aactcatttt attttttctt
     7681 tttcttcttt ttaacacctt tgtaaaatgg cttcttccct tttttcttac cgaacgtttg
     7741 cttactatcg ctatatcgtt tgttcttctt gccatttctt tctgggcgtt ttcctgcttg
     7801 ttttccttcg cgttttcctg attttccatt tcgtgttttt ggccgttcca acgctgtaac
     7861 ctcttctgcg gaaacaagtt caaaatctac tgctcgttca tctggatcag ctttgactac
     7921 ttttactcgc actttctgtc cgattttaaa agtcagacca gttctttcac ctacaagagc
     7981 taaatgattt tcaacaaaat ggaagtagtc ctgtttcaat tcattgatat ggatcaatcc
     8041 ctctactgta ttcggcagtt cgataaacag gccgaatttc acaacagagc tgatgatccc
     8101 atcgaattct tcccccactt tgtccatcat gaattctgct ttcttcatgg aatctacttc
     8161 acgttctgtt tctactgcac gacgttccat cttagaacta tgatctgcaa tttcaggaag
     8221 ttccgtagcc cacgaacttt gattgctttc acttttatct tcactgtaag aacggatcag
     8281 acgatggacg attagatcgg gatagcgtcg aataggagat gtaaagtgtg tgtagtaatc
     8341 tgccgctaag ccataatgcc caaaattgtc ttctgaatag cgtgcttgtt gcatgcttct
     8401 tagcaacata gtattgataa ctgcagcttc tggtttgtct tctactcctt cgatcacatg
     8461 ctgtaaatct tttggtgtga tcgttccttt cgttcctcgg accagaatgc ccaaagctga
     8521 agcaaaatca aagaagcgct gcattttttc ttcttttggc tgttcatgga ttcggtaaat
     8581 aaacggcaga tttaatttat tataatgttc agctaccgtt tcattggccg caagcatgaa
     8641 agattcgatc aagcgttcac caattcctct gcttcttagc tggatatcat ttgggtgtcc
     8701 ttcagcatca acaaggattt ttgcttcgcg atcctcaaaa gaaatcgcgc cacgtttgat
     8761 tcgcatcgtt tcaaggattc gatgcaattc acccatttct tggaacatag gtacgagctg
     8821 agcatagcgt tgcatcgttt cttcgtcttg atcttctaag atttgattga cagctgtgta
     8881 ggtcatccgt tctgttgttt ggatcacact tgggaaaata tcatgagaga ctacttgtcc
     8941 atttggatca atctccattt cgcaactcat ggtcagcctt ggaacatggg gattcagtga
     9001 acagatacca ttcgataaac gttgcgggat catcggaatc actcgatcag tcaaatatac
     9061 acttgtccct ctttcatatg cttctctgtc caattgactg ccttgtgtca catagtaaga
     9121 aacatctgca atgtgtactc caagaaaata attaccgtta tctaattttt taaccgtaac
     9181 tgcatcatct agatcttttg catcttctcc atcaatcgta acaatgagct gatttcttaa
     9241 atctctacgt ccagttatat ccttttcgct gatttgatct ggaacttcat ctgcttcaga
     9301 caatacatca tcaggaaatt tcgtagggat accattagcc acgacaattg agaggatatc
     9361 catgccaggg tcattcttat gaccaatcac ttgcttgatt atcccttcta aactagtcgc
     9421 ataccctttt tcagggtaat gggtcagttc gatcagcaca atacttccgt caactggacg
     9481 aactccctct gctgctgcat atactttaaa ttggttgagc tttctatctt tcgggataac
     9541 aacgccgtaa agatccgttt cttgtatttc ttcttctgaa taagcaacga attcccctgc
     9601 taactgagta gtcgcccggt ctcgaatctc gactactttc ccctctgctc cgcgatccat
     9661 atctgtattt gctcgcttca tgatatcgat tgctacggtg tctccatcca ttgcaaaacc
     9721 agtcgcttct tttgggatat agatgtcatc ttcttcggga tcaatcgtta caaatccaaa
     9781 tcctcgttca tttgctcgaa atgttccttc gatcaagatt ggttttaaag gaagtttcac
     9841 ttttcctttt ttattgaaaa ctatactctg ttctctttcc atttgagcaa tcgtttgtac
     9901 tagtaattta aaatcatcgc ttttttggaa ccctaaacct tcagcgattt cttccataga
     9961 aaaactgcgt ttgcgactgc tttccatgaa aaagagaatt ttctctttta tcgtttcttt
    10021 tgtcatattt attcctcatt ccaaggtaat ttttctaaaa attccaagac gtctttttct
    10081 aattcgcgtc ttgctgtacc aactgtaatc acatgaccac tttcaggata ccatcgaagt
    10141 gtcactcgct gacggctcaa cttgcgtgca gtttcaaata cgccattagg gtcgatcatt
    10201 tcatctttcc cagcttgagc taaaaatatc ggctgttgga tatctgacaa cctactttct
    10261 acgattgctg cctgatcttg tatatccatt aattgttgtt cagctaacga gcggtaagca
    10321 actgcttttt cattgatttc tttctctgat ttacctgctg tcttcagcac ttgacgtacg
    10381 tatttttcaa agttttcagg cacgtttgtt tttacaggag acaaaggcga acagaaaaaa
    10441 ccgccgccaa tcactgattc ttcttctagt gctcgtacag caaagatgcc gcccattgat
    10501 aatcctaata cggcaatatc agaaaactgc tcagactgta aaaaatggat ggctttttta
    10561 gtgtctgccc accagctttc tgctttttct tgcaaaatat catatggctc cattgttccg
    10621 tgtcctttaa aaagcggcgc ataaactgta taatctgctt tttccaaaaa tcgtgcgagc
    10681 attcgaacat cgtttggact tcctgagtaa gcgtgaagca gcaaaaccgc tcgctttcca
    10741 tgttttgcat aaaggggttt tggtaattgt ctcactcgtc tcacctcttt cgcctcattt
    10801 taccatagtt tctttcataa cttcttctta agcgttctct ttgcaataaa aaaagccgcc
    10861 cgccgttttg cgagaggctt attcaactac ttcgaagata aatatgctag gacaaataat
    10921 aagatcatcc agatagctcc cataactgca gttgatcgct gcatgacagc ttcaaagccg
    10981 cgagcttttt gtttgccgaa tagttgatcc gcaccgccgg taaatgcact cgctgcacta
    11041 ttttgcttgc ttggctgcat cataatcgca atgaccataa caatcgataa aacgatcacg
    11101 attcccaaaa ttaaattata catatcgcag cctccttccc aaaagatatc gtctctttaa
    11161 ctttatcata gataaaagtc cgtgacaact ctatataaat ggaataacag ctgaaagttc
    11221 aaaactttca gctgttattc ttatgactga cgtaagacgt cttgatattc tggtttcttt
    11281 tcaaactctt tttttgcata agggcataaa ggaatgatct ttttcccgtc tcgtcttgct
    11341 aattctactc cagtatacac tagcttttca gctaactttt gaccacgata tgctggatct
    11401 acaaatgtat ggtcaatgat catgatgtct gggcccgcat ctgaccatgt catttctccg
    11461 atctcttgtt gttcatcatt gaataaagca agacgatttt tttcttcttt gatttccata
    11521 tgccgcactc ctcaaataac tgatattttt ttattcctac cttattttag cagtctcctg
    11581 atctataaac aattatttat cctcgaatta cttacaaaaa agaagagaaa attaattcat
    11641 cgttattctt tgttcaataa atgaaaaaat cactgtccaa taggttttcg gatctttcat
    11701 cgatgcttcc aagtgttctg cttctggaac aagcagcagt gctttatccc ctgccgtagc
    11761 attttgaatc gtgtacgctg cttctgtagg gacaaaatga tctctttcac catgaatgac
    11821 caataaaggc agatggttgc tgccaagctg tctcgtagct gatgcttgct ttaaggagta
    11881 accggcgtat ttctgggcat aatgattcgc atatctcatg accatttttt taggaaagac
    11941 agtgatttta cttaacatat atcggaattc tgcataaaca gaaacatatc cgctgtctaa
    12001 aataaatcct ttgaccgctg atggaagttt ttcaccactt gccatcatga tcgtagaagc
    12061 tcccattgat ccgccgtgta aaataatgga cgcattcggc tgttcatcta agatgagttg
    12121 gatccaggca attaaatcta gccgatctag ccatcccatt ccgatgattt ctccttcact
    12181 ttctccgtgc gcacgtaaat caggtacaag aacgttgaat cctttctctg catatctttt
    12241 acctatatga gacatgtctc ttttccctgt gcttcggtaa tcatgtacgc aaatgaccca
    12301 tttattctgc ttatttggat ggatgaacat ctgaccagct agtttcaacc cgtcataact
    12361 tttgattgtt ttgggtgttc cctgctccca aaagctttca cactcttctt ctaatttttg
    12421 tcgttttttt tctactgacg tgactgcagc agggtccatc aaacgatgac cagtgtcttc
    12481 ataccagtca tatcctctct gtactcccgc tcttactaat ttctttgcat aaaaataaga
    12541 aagaaaatgg taactgatcc ctataattat cagaataaga atgaaataaa aaataaccat
    12601 tgtacacccg ccgctttctg ccaaactata atctaactat tatccaatgt accgatccat
    12661 aggattcttt attttcggca cacgtaaatt ccatttcttt ttcattgtat catttactta
    12721 aagttctcct caatagattt acttgtaaga gagcatttac attcgcaata aaagccagta
    12781 tactaatttc gtgcattata atcaagacgc tcgtcttgga ctggttcgga aacttcccag
    12841 aataaaaaac taagtatctc taaagaaagg agattggaaa taatgcgaaa agtaattatt
    12901 gattgcgatc cagggatcga cgacacttta gctttatcat tagctgtcaa atcccctgat
    12961 atcgaagtag ttgctattac cgtagtttgt gggaatgtcc ctgcagatat aggtacgcag
    13021 aatgtgctga aatgcctgga acgctgtgat cgtttagaca ttcctgttta tcaaggaatg
    13081 gaaaaacctt tgaagcaacc tttcatcagt gcacaagaca ctcatggtat ggatggttta
    13141 ggggaaacaa atttcccgat gatcttgaga aaacaggcag aacctctcca tgccgttgat
    13201 tttttggctg attatttcaa agaaaagaca aatacatcag tcattgcttt gggtcctttg
    13261 accaatatcg cttttgcttt gaaagtcaac ccaaatattg ggaaacatat ggatcgtttt
    13321 gtctcgatgg gtggaactta caaaagccat ggtaactgtt cgccagttgc tgaatacaat
    13381 tactggtgtg atcctgaagc agcttcttat gtattcgaga atctcaaaca aacgatagaa
    13441 atggttgggt tagatgtgac aagagaaatc gtcttgacac cgactatcct cgaatactgc
    13501 tgtcaaatga atcctgaaga gggagagtat ctaaaagcga tcactcgttt ttattttgac
    13561 ttccactgga aacaagaaag aattttagga tgtgtcatca atgatccgtt agctgttgct
    13621 tattttatcg agggggccat ctgtgaaggt ttcaaaagct tcactgctgt ggaaactcaa
    13681 ggaataagtc gtggacaaac attggttgat cgttatgaat tttggcagaa acctgctaac
    13741 agtaaaatta tgacaaaagt agatactcgt ctttttttcc gaaagttttt aaccgttctt
    13801 ttaaatgcac aagaagaaac gattatgaaa gatttggaaa ggttaaagat gggctaacaa
    13861 aatgaataaa aacaaaatgg caactcgcgt acttacagtg atggctttgt gtattgggat
    13921 caattatatt ggaggaacga ttgctttatg gcttcgtttg ccaatctatc tcgattctat
    13981 cggaacgatt tttgctggtg cattgcttgg gcctgtacct ggaatgctga caggattgtc
    14041 cagcggtatt ttaagtggag tgactacaga tattttttct ctgtattatt cgccaatcca
    14101 aattctcaca ggcttgcttg ctggattgat tttgccaaga tatctcggca aagataaaaa
    14161 cagcttgaaa ctgttattac tcgctttgat tttgtcggta cctggaacga tcgtttcttc
    14221 gatcattacc gttcagttat ttggcgggat cacctcctca ggatccagta tgatcgttca
    14281 gctattacat gggttaggtt taagtcagac aacaagtgtg atcttggtac aggcaggtac
    14341 agactacctt gatcgtttgt tgtctatcgt cgttgtttca ttagtcatca ctatgttgcc
    14401 acaacgcttg atatattcaa aaagaaaaat aggctgaata aacagagctg gattagcaaa
    14461 ataatgctaa tctggctctg ttttttcatt tattaaaaaa atcttacgct aagtaggata
    14521 aaataattcc ttgatatttt tcaatcatat ccaaataatt atcgatttca acatactcat
    14581 ctacttgatg aggcaaagta acgacaccag gcccaaagac gacaaagtcg aaggaatggt
    14641 tagctttcgt aaattcggct gcatccgttg ttgctgccgc tccaactaaa ggtaatggct
    14701 ggctgaattg ttgctgaatg caatgaatca aaggagaatc tggatcagct ttgacaggta
    14761 ttttgttgta atcgatcatc aattccaaat gatagtctgt ctcttgattc aattcattga
    14821 cgattgattg cagcaatgca atgattttat catttgggta ttctggaatc gatcgaatgt
    14881 tcccttggag ctgcgcatgg cttgggatgc tgttgacttg attcccgcca ctgatcaatg
    14941 taacattatg gattgttttt cctaggacag gattctcgat tgtttcagcg aggtgattca
    15001 tttcagcatt tgctttcgta atgaattcat ttaggtgatt gattgcatta taaccttggt
    15061 ctggcataga gctatgcgct tcttttccgt gcgaggtgac tgtatagttg attgatccca
    15121 tgtgcgtata catcaaactg taattagttg gctctccgat gatcaaggca tctaagtcat
    15181 ctacatagcc tgctttcgtg agctgttccc cacctagttc acccacttct tctccgacag
    15241 tggctaataa tttgactgtt ccgttaaatg gttttcctga ctctttcagt tcgatcatgg
    15301 caatcaccat tgctgccaag ccactcttca tatctgtagc accacgtccg tacaaacggt
    15361 tcccatggat ttctgctgca aagggtgcat aggtccaaga cgattcatca ccagccgcca
    15421 cgacatccat atgcccagat aggcctaaca cttttcctga ttggcccttt tggtaacgag
    15481 caatcaaatt atctctacca tctctgtaag aaacaatctc tcctgttatg tcatgctttg
    15541 ccaaaagctt attcaaatag gctgctactt caccttcgtt tccattcact gatttaattc
    15601 ggatgatctc ttgtaaaatc gcgatttttt cttctttttt catctaatcc tctcccttgc
    15661 catttctttt tattgtattt gagtgtgctc gaattttcaa ataggagcaa ccaaagtaaa
    15721 aatgaagaaa gagagccact ttatgaaaaa tatgctgcta cttccctcct attttattta
    15781 tacgagagga cttgctcata gattcctatt ctactgttct atccttaaaa gctcttatat
    15841 gatgctagtt attgttagct ttcctttttc aaggatgatc ttttcttgta ccattctttg
    15901 taaacatcat atacagaagc tagttgcaaa aaaaactctc tacctgctga tattt
//
LOCUS       DAZULY010000044        15896 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000044 DAZULY010000000
VERSION     DAZULY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.044"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACG81A_000934"
     CDS             226..894
                     /locus_tag="ACG81A_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFS5014323.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="ACG81A_000935"
     CDS             complement(1094..1480)
                     /locus_tag="ACG81A_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014324.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="ACG81A_000936"
     CDS             complement(1452..2834)
                     /locus_tag="ACG81A_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFS5014325.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="ACG81A_000937"
     CDS             complement(2990..4177)
                     /locus_tag="ACG81A_000937"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFS5014326.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="ACG81A_000938"
     CDS             complement(4184..5215)
                     /locus_tag="ACG81A_000938"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS5014327.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="ACG81A_000939"
     CDS             complement(5231..6685)
                     /locus_tag="ACG81A_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFS5014328.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="ACG81A_000940"
     CDS             6860..7765
                     /locus_tag="ACG81A_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFS5014329.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="ACG81A_000941"
     CDS             7856..8533
                     /locus_tag="ACG81A_000941"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS5014330.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8530..8700
                     /locus_tag="ACG81A_000942"
     CDS             8530..8700
                     /locus_tag="ACG81A_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014331.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8814..9710
                     /locus_tag="ACG81A_000943"
     CDS             8814..9710
                     /locus_tag="ACG81A_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5014332.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="ACG81A_000944"
     CDS             complement(9765..11135)
                     /locus_tag="ACG81A_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5014333.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="ACG81A_000945"
     CDS             11373..12119
                     /locus_tag="ACG81A_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS5014334.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="ACG81A_000946"
     CDS             12116..12955
                     /locus_tag="ACG81A_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFS5014335.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="ACG81A_000947"
     CDS             12956..13906
                     /locus_tag="ACG81A_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFS5014336.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="ACG81A_000948"
     CDS             14047..14799
                     /locus_tag="ACG81A_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014337.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..>15896
                     /gene="queG"
                     /locus_tag="ACG81A_000949"
     CDS             14818..>15896
                     /gene="queG"
                     /locus_tag="ACG81A_000949"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFS5014338.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILA"
BASE COUNT         5144 a         2992 c         3204 g         4556 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagc
//
LOCUS       DAZULY010000045        15767 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000045 DAZULY010000000
VERSION     DAZULY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.045"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..844)
                     /locus_tag="ACG81A_000950"
     CDS             complement(11..844)
                     /locus_tag="ACG81A_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFS5014339.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(1148..3313)
                     /locus_tag="ACG81A_000951"
     CDS             complement(1148..3313)
                     /locus_tag="ACG81A_000951"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFS5014340.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            3573..5867
                     /locus_tag="ACG81A_000952"
     CDS             3573..5867
                     /locus_tag="ACG81A_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFS5014341.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5860..6525
                     /gene="pgmB"
                     /locus_tag="ACG81A_000953"
     CDS             5860..6525
                     /gene="pgmB"
                     /locus_tag="ACG81A_000953"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS5014342.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            6518..7525
                     /locus_tag="ACG81A_000954"
     CDS             6518..7525
                     /locus_tag="ACG81A_000954"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFS5014343.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            7547..8566
                     /locus_tag="ACG81A_000955"
     CDS             7547..8566
                     /locus_tag="ACG81A_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5014344.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(8847..10214)
                     /gene="nhaC"
                     /locus_tag="ACG81A_000956"
     CDS             complement(8847..10214)
                     /gene="nhaC"
                     /locus_tag="ACG81A_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS5014345.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(10324..11745)
                     /gene="tyrP"
                     /locus_tag="ACG81A_000957"
     CDS             complement(10324..11745)
                     /gene="tyrP"
                     /locus_tag="ACG81A_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFS5014346.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(11988..13865)
                     /gene="tdc"
                     /locus_tag="ACG81A_000958"
     CDS             complement(11988..13865)
                     /gene="tdc"
                     /locus_tag="ACG81A_000958"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS5014347.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(14156..15412)
                     /gene="tyrS"
                     /locus_tag="ACG81A_000959"
     CDS             complement(14156..15412)
                     /gene="tyrS"
                     /locus_tag="ACG81A_000959"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFS5014348.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(15524..15729)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         4754 a         3398 c         2882 g         4733 t
ORIGIN      
        1 gttatatttt ttattcgttc acatctaatt cgatttccac cccgtagtga tcactgatga
       61 ttggactatc gatcccatca aaaatccgtt gatatttttt aacctgcatt ccttcaggga
      121 cgaaaataaa atctatacgc aatttttctg tattttgttc ccaaccatct attttctttt
      181 ccacagtagc tgcttttcct ttttcattcg cgacaacata cgaatcttga agaagaaggc
      241 aactttctct tacagtataa taaccttgag agtctactgg attattgaaa tcacctagga
      301 aaaaaagtgg tacgcgagtt tccaaaaagt agttttccaa gtttttccac tcaaaataaa
      361 agccactttt tttatctgtc caccaagaaa agtggcagct cgagacaaaa atgtcctgat
      421 catctatctt tgttttccca atcaagatct tgcgtgtctg ataatcttct ttcttttgag
      481 attgggagac ggtaaggacc tgactttcca atgttttttt cgtaagcagt gcatttcctt
      541 cttcatattt atcataaccg atgtggctca tttcccaact ccaaaaatac tcttttccgt
      601 gttcctttaa aagctgaact aaacggtagg caaaattatc tttacggata ggtacttgat
      661 tctttacctg acagaacttc attagcttcg caggttctac ttcttcagat tccagtaatt
      721 ggttgatttc ttgtaaagca atcacttcat aatcttctaa caggatctta tctgctaatt
      781 cttgcaattt tttctctggt tctggctcaa gccagctatg cgtgttgagt gtcatgaatt
      841 tcattggttt tctccttgtc ttcatttcga aacaaaaggg ttgtgacaaa atttttgtca
      901 caacctctcc agcaaatagt gttctcctgc taactcctca gtaataagct caatggacca
      961 ggagctattt tttgtttggc tgtttttatc acttggtgca gggcagcttt ttcacgacct
     1021 ctccactaaa cagtattcaa actgctgctt ctcggtaata agcccaacca aacgaaaaat
     1081 aggaggagct atttttgttt ggctgttctt atgacttgat gcaggacagc tttttcacaa
     1141 cctctcctta cattttaatg gagccgatca ctttaccagc tgtcactgta cctaattgct
     1201 tgatcactac ttcttctact ttatcgtttg taaatacgat catgatcgtt ggatcttttt
     1261 cttcgttttg gatcacatca agatccatct cagctaattt cgttcctgtt cctacttctt
     1321 ggccttcttt gacagcaatc tcaaaagctg gctgattcat ttggactgta tcgattccca
     1381 tatggatcaa aacttcgata ccatctttcg tttccagccc aattgcatgt ttgctaggga
     1441 aaatacttgt cacttttcca gcgatcggcg caacaacttc tcttgatgtt ggaataacag
     1501 caaagccgtc acccatcatt ttttgtgaga ataccggatc ttttacttca gataaaggaa
     1561 tcacttctcc agaagctgcg gaaactagtt cttgttctat acctaagtat tgcttactac
     1621 ctgcttgtcc atttcctgta gaaaccattt ctggctctgg aatctctgct cctgattgca
     1681 gcagatcttc gatatctgat ttcaagacat ctgcttttgg tccataaact gcttggactc
     1741 cattgtcttt gacgatcaat cccatcgccc ctgctcgttt ccaggcttct tcagtcccta
     1801 ctttttctcg atcatttaca cttacgcgta accgagtcat acaagcatcg acatctttga
     1861 tattttcttt tcctcccagc aaatgaatga tctggaccac ttgctgatct gctgttcctg
     1921 ttccgctgtt agatacaacg gtttcttcac tgtcattgtc gtaatttccg ttacgtcccg
     1981 gtgtagcaaa gttgaatttt ttgatcaaga aatttgctaa gaaatacgta acaacaccaa
     2041 aagcgatcac acacaacacg aagttgataa gatctccacc tagacccgct ttgatagcta
     2101 acggtgtccg agtcagcaat tcgatattcc caaatgaatg gacccgcaat ggcaagatat
     2161 ctgccatcgc aaatgcagcc ccttggatca ctgaataaat gacatataat ggcacagcag
     2221 caaacataaa catgaactcc aatggttctg ttacacctgt tagaaagaca gccaacgctg
     2281 ctgaaaaata cattgattta tatcttgctt tcttatcagg atcgacatta cgatacatcg
     2341 caaaagccat tcccatcaag atacctgaag aaccaatcat ttgtcctact ttgaaacgag
     2401 ccggcgtcca gttatgcaac acaaactcat attgcgacat atcccctgca cctttaagat
     2461 tcactaaatc agtggcccaa gccaaccata acggatcttg cccaaatact tgtgtccccg
     2521 cctgtgcgcc tgataaaatc tcatacgatc cgccaagctg tgtatagttg ataggaatcg
     2581 tcagcatatg atgcaatcca aatggcaata gtaaacgttc taatgttcca aataaaaacg
     2641 gtgctagaat cggtgcgctt tcttgtgatt ctgcaatcca taaaccaaaa ttattgattc
     2701 cagcctgtac gtttggccac accacagcaa gaatcaatgc cacgattgtt gaccataaga
     2761 taacaacaaa cggaacaaag cgctttccat tgaaaaatga caaagcatcc ggcaattttc
     2821 tataattata gtacttatta taagccattg ccccgacaaa tccagcgatg atccctacga
     2881 atactcccat attcaaagct ggtgcttcca atacacttgt aaaaaatcct ttaaccatga
     2941 tcttcgtacc gaacagagtg tgggtaaatg catcttcatt cgcaagcatg tcacttgtga
     3001 caccaaagat agccccagtg atccgattga ttaaaataaa tgagatccca gctgcaaatg
     3061 ctccgcctgc tctttctttt gcccaacttc ctcctattgc taaagcaaat aaaatatgaa
     3121 gattaccaat aattgcccaa ccaatatttt caattactcc gcccgttgtg acaagcggcg
     3181 tccaatcagg attgatcaag ggaattgatt tccctatact gatcatcaac ccagctgccg
     3241 gcataactgc taccacaacc attaaagcct ttccaaattt ttgccaaaac tcaaaactaa
     3301 atatcttttt cattccgttt agctccttta cgcaatcggt ttcgttaaac gtattatagc
     3361 gtgcgttttc ttttttgtaa atgctttatt ttttattttt ctgtctatac cattctataa
     3421 aacctataat acgtgtgttc ccttctataa ttttctccta aaaatttatt tttttaagct
     3481 tttttctatg aaatattttc ttttgtctat tgacttgtgc aatcggttgc gtttattatg
     3541 aacgtataaa acagctcatt aggaggagaa aaatgaaaca gatcaaacga ctatttgcta
     3601 ttgatccatg gaaaatcagc acgacacaat tagataaaaa aaatcttcga ctgcaagaat
     3661 ctttaacaag tattggaaac ggttatatgg ggatgcgcgg gaactttgaa gaaacttact
     3721 caggggacca ccatcaaggt acttatttgg cgggtgtctg gtatcccgat aagacacgtg
     3781 tcggctggtg gaaaaatggt tatccagaat actttggaaa agtgatcaac gccttgaact
     3841 ttatcgcgat agatatttcg atcaacgatc agctgatcga cttggcttct ttggaaccag
     3901 aagactttta ctgggaatta gatatgaaaa atggtgtatt atctcgtaca ttcacaatca
     3961 ctacttctac taataaagta cgcatttcct tcgaacgttt tctgagcatt gtcaaaaaag
     4021 aatcagctta tatctattta actatagaaa tgcttgaagg tagcggaaca gtcaaactcg
     4081 tatcaaaatt ggatggaaat gttcaaaacg aagacagcaa ctatgatgaa catttttgga
     4141 atgagattga tcgtggaact atagaagaaa tcggctattt gacgactaaa actattccaa
     4201 acaactttgg gattgaacaa tttactgtta ctgcagcaat gcgccatttc gttaatggaa
     4261 aatcagtcat ccctgactat cacgaagata ctttagcatt acatggttgc ttgtctgcag
     4321 aattatctca aggtgagaca tggtcagtgg aaaaagaaat tatcgttctt actagtagag
     4381 atattccaga agcgcagcaa gcagaaaaag ctgtctcaga attaacgaac cagacaaaca
     4441 attatatcca agcaaaagaa gagcaaacag cggcttgggc agaacgatgg aagcttgccg
     4501 atgtggtcat cgaaggagac gaagaagcac aacaaggtat tcgattcaat ttgttccaac
     4561 tattctctac ttactacggc gaagatgaac gattgaatat cggccccaaa ggattcacag
     4621 gagaaaaata cggcggagcc acttactggg atacagaagc gtatgctgtc cctctttatc
     4681 ttgctttagc caaacctgaa gtaactaaga atctgctgaa atatcggcat aatcaactgc
     4741 cgcaagcaat ccacaacgct cagcaacaag gattaaaagg cgcgctttat cctatggtca
     4801 ccttcactgg tgtcgaatgc cataacgagt gggaaatcac attcgaggag attcaccgaa
     4861 atggcgcgat tgcctatgca atctacaatt atacgaatta caccggcgat acggaatatt
     4921 tgaaaaatga aggactggaa gttttagcag aaatcgcacg cttctgggca gaccgtgtcc
     4981 atttttcaaa acgagcaaat aaatacatga tccacggtgt gaccggccca aacgaatatg
     5041 agaacaatat caataacaat tggtatacaa atacgatcgc agcttgggtc ttgcgttaca
     5101 cgcgagaaaa ttatttaact tatcaaaaag aagctgctat cactatcagc gaagaagaac
     5161 tagccaaatg gaaagatatc gcagagaaca tgtacttccc agttgacgaa gaattaggaa
     5221 tctttgtcca gcatgacaca ttcttagata aagatctcat gcctgtatcc gatattccca
     5281 caagcgatct tcctctgaat cagcattggt cttgggacaa aatccttaga tcttgcttca
     5341 tcaagcaggc agatgtacta caggggattt atttcttcaa tgatactttt acaaaagaag
     5401 aaaagcaaaa aaactttgat ttctatgaac caatgactgt ccatgagtct tccctttcgc
     5461 caagtatcca ttcgatttta gcagcagaat taggaatgga aagcaaagct gtagaaatgt
     5521 accagcgaac agctcgcttg gatttggata attacaataa cgacacagaa gacggcttgc
     5581 acatcacttc catgactggt agctggttgt cgattgtcca aggatttgct caaatgaaaa
     5641 ctgcccatga tcagttgaga ttcgcaccat ttctgccaaa aacttggaaa gcttattctt
     5701 tccatatcaa ttatcgcgga cgtttattgt tcatctcagt cacagatgaa caagttactt
     5761 tcgagttatt agaaggtttt cctctagaaa tttacgtcta tgaacagtct tattctttag
     5821 cggatcgtct agaaataacg ctaagaaagg aggaagcaaa tgtttaaagg ggtattgttt
     5881 gatttagacg gtgtcattac cgatactgct gaattccatt atcgtgcttg gcgaaagctt
     5941 ggtcaggaaa tcggcattac gatcgaccac acattcaacg aacagctaaa aggcgtcagc
     6001 cgagaagact cactctcttt gcttttagct tatggcggaa aagaacactc tttttcaaag
     6061 gaagaatttg cagaattagc gaaaagaaaa aacgattatt atttagagat gatccagacg
     6121 atcgaaccaa aagatgtctt ccccggggtc gtgcctttac tggattcttt aaaagaagaa
     6181 aaaatcaaga ttgctcttgc ttctgcaagt aaaaacgggc cattcctact tgaaaaaatg
     6241 ggtcttacac cttactttga tgctattgcc gacccggcag aagcaacaaa cggaaaacct
     6301 gcgccagata tcttcctcct tgcagcaaaa gctgtcggac taaaagcaga agaatgtatc
     6361 gggatcgaag acgcgcaagc cggtatccaa gccatcctct caagtggcgc tcaaccaatt
     6421 ggcgtgggac gtgctgaaga ccttggagag gagatcccgc ttgtccctga tacgactttt
     6481 cttacgatcg attacttgaa agaaaagtgt catgaccatg gataatccag agatctccgt
     6541 cgtaccagtt ccagagactg aaataaagaa aatcacattg aagaacgact acttagaagt
     6601 cgttcttctc aattacggtg cccgcctgca ccaaatcttc gcaccagaca aagaaggaaa
     6661 atcagaaaat gttttattgt cttacgatcg tttcgaagat gttctaacag acaaaagctt
     6721 ttttggagca actgtcggac ctgtagccgg aaggatccgg gatgcttctt ggggtgagca
     6781 tagattggag aaaaactgcg gatcacacca tatccacggt ggcacgaaag gctggtcttt
     6841 ccaattttgg gaagtagaag ttttcaaaac tccatcatca ataggtgtgg tcttttactt
     6901 aaaagatgaa ttttctacct atccaggacc aatcactgct acgatcactt accgtttaac
     6961 aaaaaacgaa ttagagatga caacctctgc tagtagcctt gttgaaacca tctgcaatcc
     7021 gaccaaccat gcctacttca acctttcagg aaatggaaca agagacatct atgaacatca
     7081 gttaacggtc tttttagatg gtgtgcttga attagatcaa gaaaaactgc ctactggaaa
     7141 ttggacaaaa aaagaaaaac tgccaatcga ctttagaaaa tccccaacac tgcaagagat
     7201 ccttgcatgt tatccagatg gactagatga tgtatttctt ttgcatcatc ctaggctttc
     7261 caaaacttcc ttacaattat tcgagaagca ttctggtcgc caaatgacta tagcaacttc
     7321 taataaaagt atggtattat tttctacgac tggattcgaa gctgatttct ctgtcaatgg
     7381 aaaacagatg cattcaaact acggattagc aatcgaaccg caagaaatcc ctgatatcgt
     7441 tcatttcccc aaatttggat cgatcaacct ccatcctggt caagaacgga tcagtcagac
     7501 gatttatcgg ttctctgcac aataacaaga gaaaggtgtt tttatcatgg ctattaccgt
     7561 aaaagatgtg gcgaaaaaag ccggtgttgc cacctcgacc gtctctcgtg tcatcaacga
     7621 tcatcctagt atttccgaga caacaaagaa gaaagtccga aaaattatgg atgaactagg
     7681 ttatgttcct aacatgactg cccgcaatct aggaaaacgc atttccagtg ccatcggcgt
     7741 aatcttaccc cctcttgatt caaaagagcg gatcggtaac ccattttatc ttgagatcat
     7801 ggaagcaatc aacgaagaag cgcggaatta tgatatgact acagcaatcg ctactgctaa
     7861 aaattttgaa ttgctgttag agaacgtcca gcgtatgcat ttacaaaaac aagtagatgg
     7921 ttttattcta gtgtattcag atagtcaaga tcctgtcatt gattatttat accaaaacca
     7981 catccctttc actttgatcg gacagcctta tcatcatgag aacgaaatcg tttatgtaga
     8041 taatgataac cagcttctag gtaaacaagc aacagaattt ttgatccaga atggacataa
     8101 gaatatcctg tttgtgacaa acacgactca tgaaagtttg tatttcgaac gatatttcgg
     8161 ttatcaaaaa gcaatgatga tggctggtct tcctgcgtta tccagtgtag tactagagca
     8221 tccggaggat tatctgtcct ttgagaaaac gttgaaagaa aacgaagcca cagcattggt
     8281 ggtcattgac gatatcttcg cccttcgcac gatgcagttg gcgcaaatgt acggctatgt
     8341 cattccagag acactctccg tcattagctt caacaactcg atcttctcaa cattaacaca
     8401 cccttacctt acaagtatcg atatcgatat ctccgaatta ggacgtgtcg ctatgcaaaa
     8461 gctgcgcgaa ttgatccatg aagaattgag cagtggcgta cgtttggtca tccctcatcg
     8521 cttgatcaaa agagagacag tcgttccttt agaaaaccca tactaaatag ctgactcact
     8581 aaatacgctg accatttatc ctatggtcag ctttttatgt aaaagaagat actacttcta
     8641 acacattgtt tatctgtaaa aaagagcgtg ggacaaaagt aaagaacact tttgtctcac
     8701 gctctaaaca cgtataaacg gcggaagcag aagcaactcc ttcggaaata agctgaaatt
     8761 cacaaaaatt taaaaagcaa ttttcttgag tttcttctta tttcttggag ttaaacgctt
     8821 ctgtcccagc ctcttttttc cttccttcaa ttttttacac gtcgacgatt caataaaaaa
     8881 ccgcctaata ttgtaaagac aggtgcaaga atcgagaaaa agacgaatgg caagtaatgc
     8941 aacgcgctaa ctttcatcgt tcccataatg aatgtgccgc tgactcccca cggtacgaga
     9001 gaattgacgg tcgcacctga atccgctagt gttcgtgtca aataggtttt tgataagcca
     9061 acgtcatcaa aagaacgctt gaatgtttct cctggaagaa tgatggacag atactgttct
     9121 ccaacgatca gattgattcc gatacagctc aaggcagtaa acccgactaa acgagaaggt
     9181 cggtccatct tttcttttaa ttcttgaacg atggtatcaa cgatcagata tttgatcagt
     9241 aacccaccta atgccaatgc aagtaaaatc aaactagcag aacctaacat actcatgatc
     9301 ccgcctcgag aaagcagttc atctagcttt ggatcaggtg tatttgcgac ataaccattc
     9361 atcaagatgt ctgctgcttt tccgaccgtc aaacttttct catggataaa agaaagaacg
     9421 agagcagctg tcgatcccgc tagtaatgta gggatagccg gaattttttt gattgccccc
     9481 ccaaataaaa taactagtgg aatcaatgtc caaggagaga tccaaaagct cgctttcatg
     9541 ttttggatca tttcattgat actagctaga tcggctgaat gactgttatg tcctaatatc
     9601 gtataaaaaa tcaacgaaag gacgaatgct gggaaatccg tataacgcat accagcgata
     9661 tgttcgaata gattaactcc tccaataccc gctgccaagt tggtcgtatc tgataaaggc
     9721 gagcagttat tccctaaaaa ggcaccggaa acgactgcgc ctgtagtcat tgcatcattg
     9781 aatccaagta aatgaccgat ccccaaaaaa gcaattccaa tcgtcgaaat cgttgtaaat
     9841 gaactgccga cagtcactcc gacaattgca caaacaagaa atgctgtcgg taaaaagaaa
     9901 cgcacagaaa caagtttaaa tccataaacc ataatcgtag ggatggttcc agataaaatc
     9961 catgaagcaa ctaagacacc aatcaaaaga aaaatcagga ttggaatgat ccctggagtg
    10021 attcctttga tgatcccgtc atggatctca tcccatgtga actttttaaa tctgccataa
    10081 aacaacaaac acatgaaagc tgcaaggatc gggatatgtg gcgagagctg gaatccgatg
    10141 atcagtactc ctaggagtgc caacaacacg atcaatacag cgatactttc ttttaaagaa
    10201 atatttttat tcatcgtcga tctcctattt caaatgaaaa ggccctgcca tgaaaaaact
    10261 attcacttgg caggacctcc tactaaatca gcgacaaaac gtcggctgaa agacttattt
    10321 tttttatttt tcttctaaac ttttttctac ctcaatcccc caagaatctt tcttgaagtg
    10381 attgatgatc aatggaataa cgaacatgac cactgtgata gctgctacta ataatgcatc
    10441 actcaatgtc gctgtaccta atgtagcaaa tacgacaacg atgatagaga agatcagcat
    10501 gcagctgact acccaagcta atccgtttcc gtttttacca attcggtaag gacgttcgaa
    10561 ttctggatgt ttcttacgca ttttaagcaa tgaaacagca atcaaaatgt acacgataca
    10621 ataaataaca gttgtagcat ttgtaagtgt caagaataca gcacttacgt catccattac
    10681 accataaagc aaagcaaata atgaaataac gattgattgt gttagaacga catttctaga
    10741 tacgccgtat ttgttttctt tatggaatcc gaattttggt ggaaggaatc cttcacgagc
    10801 tacttggatg attgtttttg atggtccagt aacccaagct gataattgta aaagcacacc
    10861 aataaatacc atgaagctga agatatttcc gatcacttct ggtaagccta ggatttgaca
    10921 gtcaatcaag ataggctgcg tgatatttgc taattccatt tttccatctg gtacgacatt
    10981 tgctacaagc atcgcattga tgacattcaa taaaactaat ccgatcaaag cgataaatac
    11041 gccttttgta taattttttg ttgcgtcttt caaacgaggc atgtaaacag aactcatttc
    11101 gatcccaaca aaaataaatg tgataccagc taagtatttc aatgaatcaa tatgttccaa
    11161 gtcagggaaa gcttttgacc atgaaaagtc acctagatag ccgtttgaga ctagtccgac
    11221 tttgaaggct gccaaaacac ctaaaacgaa catgatgact actgggatat atacccctag
    11281 ccatacaccg atgttcccgc cgacttttgc catatcgaat ttcaagttca agatcgtgat
    11341 gatccagtag ataactaaaa tacaacctaa aacgaaccag tgattattgc caagttctac
    11401 attaccaaat gtgtttccta acaatggtcc taaagtagaa gcgaccatta ccatacctgg
    11461 gaacatttgc acccataata gccatgcaac gacaaatccc catttgctgc ccagtgctgt
    11521 ttttacccat aactgaggac cgccttcttg aggaagcatc gtggaaagct caccggaaac
    11581 cattgatatc ggaccggcaa aaaacaaaac tgcaaaaatt gaataaaaaa tcaatgtcca
    11641 accgactgcg gctaacgtag gaatactccg aaccgttgca cacagtgcca ttgtcagacc
    11701 gataaaggtc ccaagaccca ttttacttac ttcagattta ctcaccgttg acactctcct
    11761 tgattttttg gataaaataa cggtgtcttt gaagtagttt cagcaataaa cgaaacaatt
    11821 cagcgaatat ttgaggggat tctttgagtc tattgcttga agggtttctt caaagacaac
    11881 ctcaaaacaa agggatcgaa gagcttaggt ctccccttgc tcttcagatc caaacatgcg
    11941 tcagaaacag ttagtattca agctgttttc taacatgatt accttgctta ttttgcttcg
    12001 cttgccaata attggtctgc gtagattttt tccaattttt cttgaagagc tgctttgatt
    12061 ttttcagcat attcttcaaa gtgttcttct ttgttcatgt atggtgtcat tacgctggca
    12121 cgcaatacag ttacttttcc ggcacggttc cattcttcat ctgagaatcc tagttggttc
    12181 acaaattgta atgggctgtt gccatagtct gggatagcaa agtcagtatg tgaagttaag
    12241 aactcattac cgtaaatgct tcctttcacg tatgaagaat aatcatatac gtcatggttc
    12301 aatttattca ttgcaactaa gtcatcgttg cctttttctt tgaatacata gtctaccata
    12361 ttgaaatctg gataagttaa tggatgaact tcgatttctt tgtctcctac tttaaatgac
    12421 aagtcattca agaagttgta gaaacgatgt gcaccttcga ttgatgcacc cattaattta
    12481 ccatatcctg tgacattcaa aggtaagaca tggtgtgctg cccaaacgct ggctgctgtt
    12541 gcccctgctt ttgaaccttc caagatataa gccccaagta atgctgggat atctgcgcct
    12601 ttttcaaata catatgtcgc aaagtaagag ataacgtctc tcatgcggat atctttgatg
    12661 acgataccac cggcagaata tggaacatag cccattttat gtgggtcaat cgttacagat
    12721 tctgcttctt cgattgcttt atatgcactg tgtacttctt ctaagatata atctttgttt
    12781 tctgtaaaga cattgtattt gtaatgaaca tcttttaaat cttcaaatgg aatgaagttg
    12841 ttgtcttcgt ctaagaaaat cgcacggccg tatccgccat aagcagcatc tacgtgtaag
    12901 tagaagtaaa taccgtcttt ttctaatacg cgacgcaatg caacgatttt atcgataccg
    12961 tcgattgctc cttcttctgt tgatcctaca acaccgacta cacctaggat tggtgttttt
    13021 tcagctgcta gaccacgaac gattttttca agttcgttga tgtccatgcg atagttgtgg
    13081 tcaactggca ctggaatcac ttggtctaaa ccgataccga tgatgtcggc agcttttagc
    13141 catgaatagt gtttagtttg tggaaccaac cattttccta atttttctaa atgttttccg
    13201 ctacgtgctg agtgagcttt gatttcgtcg attttttctg ggacgctgtc taacagattc
    13261 atgatttctt ctgttgacaa gttcattaat tcccaatcgc tttttccagc aactagttca
    13321 ggtgtcactt ctttcattgc aagcggcaat gatttgatgt tgcgggcata ccaaagtcct
    13381 tctaagtttg ctaaagaacc atcagcaacg atgtggcccc agccatcttt atagctcatt
    13441 aattttgcaa attccattcc tacttcttct tccatttggc ttgttgcagg agaagattca
    13501 taggcaacgt tattaccatt ccatagcata gcaaaattgt acgctagtaa agatggcatc
    13561 aatgtttcag agttcatatg accccagtaa cgtccggcat tatgccaagg aacggaatgg
    13621 gtacgcatcc gcgctgaaat ttctgataga acatcctttg ttttattcac agtgtgttca
    13681 aaactagcac tgcttttttc ttctggcgta ataataggca tgtcttgagg catgtagttt
    13741 tgacgccagc ctaaatgttc atccaccaat tcattcaaca aagctttata gatttggccg
    13801 ttctcagctt tgtccccaat gaatagggca tttaggttta gatctttcga caatgattca
    13861 ctcatagtga aaccctccta aaaaaatatt taaattttgt cgagccctac attacacctg
    13921 ttcttctatt ctgtaaacta agttttggcc tgatttttaa tcatatttta atatgtatca
    13981 attatccgaa ttgttcacac ttttgaaata attaaaaaaa acgagggaat ttacaaatgt
    14041 aaactccctc gcaaagattg tattaacgac aaccagcgtt ctattttagg atgggtttca
    14101 aaattggaat gttgtaatag tatttccctt tttcaaggta aaaacttact aaattctaat
    14161 caattacttt ggccaaaaag taatttttct taccttttcg aataaccaca aacttaccat
    14221 cgaattcatc ggatggattt atgacaaaat ttaaatcggt aacgcggtca ccgttaatac
    14281 ttatagctcc gtttgaaaca tcttcacgtg cttgacgttt agatggttca atttttgtgg
    14341 aaacaagcag ttccacgata ttttcaggtg tgcttgaaat ttcaacgttc ggcattttac
    14401 cgaatccttg cgcgatttct tccgcattta gttctttaat atttccagag aacaatgctt
    14461 gcgttatttt ttgcgcttct ttcaagtctt cttcactatg cacaaatctt gtaacttctt
    14521 ctgccaaacg gcgttgtgct tcacgtttct caggttctgt ctctactttt ttcgctaagt
    14581 cttcgatttc ttcttgagac aagaaagtaa agaatttcaa gtatttcact acatcgcgat
    14641 catcttgatt caaccagaat tggtagaact caaatggtga agttttcttc ggatcaagcc
    14701 aaacggcacc gcctgcagtt tttccgaatt tcgttccgtc agccttcagc atcaatggga
    14761 tcgttaaacc gaacgcttct acttctggtc cttctaattt tcggattagg tctaatccag
    14821 ctgtgatatt tccccattgg tctgctccac cgatttgcaa ttgcacatcg tatgttttat
    14881 gcagatgcaa aaagtcgata gattgcagaa tttggtaagt aaactctgta aatgagattc
    14941 ctacttccaa acggctggca acgatatctt ttgctaacat cgtgttgatg ttgaagtttt
    15001 taccgtagtc tctcaaaaat tcaagtaaag agatttttga caaccagtcg tagttgttca
    15061 caaatgtgat atttgcatct ttaccaaaca attttctcat ttgattggaa agtgcgtcca
    15121 cgttatgttg cacttgttcc atcgtttgca acacacgttc tgttttgcgg ccgcttgggt
    15181 cgccgatcgt tcctgttccg ccgccaatca gaatatatgg atgatggcct gccaattgga
    15241 atcttttcat catcataaat ggaatcaaat gtccgatatg catcgaatca ccagttggat
    15301 caacgccgca ataaagtgaa atgctctttt cttcaacgag ctcgcgaagt ctttcttcgt
    15361 ttgtttgctg attaatggcg tcgcgccaag ttaattcatc aataatattc attttactga
    15421 cctccgtgta gtttttttat gaaagcagga acctgctagc atgtgtaatt aattatttct
    15481 tcaggatagg ataggaaata aaaaaagtcc cttggcatat aagccaaggg acgaaatctc
    15541 gcggtaccac ccaatttgta tgtctagata tccaacatac ctctcgaaca tttaacgctg
    15601 tcgcgtggca atcactgcca aagactcctg aaatgtaatt cgctttaaag catgtactgg
    15661 tttgcagcga ccaccagctt tctgaaacat tggctttaac aactacttcg tttcgatctt
    15721 cgtcttgtta ttattaatat gtgtctatca tagagcgcaa ctttttc
//
LOCUS       DAZULY010000046        15562 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000046 DAZULY010000000
VERSION     DAZULY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.046"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..3228
                     /locus_tag="ACG81A_000960"
     CDS             61..3228
                     /locus_tag="ACG81A_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFS5014349.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            3296..3793
                     /locus_tag="ACG81A_000961"
     CDS             3296..3793
                     /locus_tag="ACG81A_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFS5014350.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            3812..4276
                     /locus_tag="ACG81A_000962"
                     /pseudo
     CDS             3812..4276
                     /locus_tag="ACG81A_000962"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            4273..5031
                     /locus_tag="ACG81A_000963"
     CDS             4273..5031
                     /locus_tag="ACG81A_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS5014351.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(5117..5857)
                     /locus_tag="ACG81A_000964"
     CDS             complement(5117..5857)
                     /locus_tag="ACG81A_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS5014352.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            5918..7171
                     /locus_tag="ACG81A_000965"
     CDS             5918..7171
                     /locus_tag="ACG81A_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS5014353.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            7493..7720
                     /locus_tag="ACG81A_000966"
     CDS             7493..7720
                     /locus_tag="ACG81A_000966"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS5014354.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            7843..8169
                     /locus_tag="ACG81A_000967"
     CDS             7843..8169
                     /locus_tag="ACG81A_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFS5014355.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            8237..8950
                     /locus_tag="ACG81A_000968"
     CDS             8237..8950
                     /locus_tag="ACG81A_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFS5014356.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            9260..10111
                     /gene="licT"
                     /locus_tag="ACG81A_000969"
     CDS             9260..10111
                     /gene="licT"
                     /locus_tag="ACG81A_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFS5014357.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            10232..12097
                     /locus_tag="ACG81A_000970"
     CDS             10232..12097
                     /locus_tag="ACG81A_000970"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5014358.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            12111..13554
                     /locus_tag="ACG81A_000971"
                     /pseudo
     CDS             12111..13554
                     /locus_tag="ACG81A_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            13662..14363
                     /locus_tag="ACG81A_000972"
     CDS             13662..14363
                     /locus_tag="ACG81A_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5014359.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            14489..15001
                     /locus_tag="ACG81A_000973"
                     /pseudo
     CDS             14489..15001
                     /locus_tag="ACG81A_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         5201 a         2644 c         3370 g         4347 t
ORIGIN      
        1 ttggaaatag attaaaacga taaaataaaa ttgtcaaaac gtcagagagg atggtggaga
       61 atgaatcaat gtgtaaaaaa gctgattgcg tcgattttgt ttgtcttccc tctcttgatg
      121 gtgacatccg tttttgccga tgtagagaaa acaaacgaat caacgcaatc agagaatcaa
      181 agttttgaca agactacttt ttcagaagaa tcgattactt ctgaaactac agaatctcaa
      241 agtgaggctt ctactaataa aaggcaagaa gaaaataaga caagtgagac ctcagaagta
      301 gctgaacaaa cgtctgatag cagtgaagcg gcactggcaa gagctgtttc agcggatggg
      361 tacagcgaag ctgccacaac agaagaactg gcgcagcttc tcgcagacga atcagtaact
      421 aagatccgac tgattcagcc tttgacttta gacagggaat tagagatcaa acgagatatc
      481 gttattgatt ttggtggatt cgctcacaat ttcggcacac accatattta tatcaatgag
      541 atacctcagt cgatcgagtt ccagaatttt aaaggaacag ctgcacatcc aggtggattg
      601 acaccaaata tcgatggaaa tgccattatc attgcttata tgaccgattt ttggggaagc
      661 agatatcatt ttacgggaga aataaaattt accgggacac ttgatttgta tgatgggtcc
      721 aaattaggac tgatttacgc tcctcgtgca actgtcacat tagatggtgt ttccggtgtg
      781 ttggatgtcc aaccagtgaa ggaaggactg aatgccgctc cgggtaaagc ctatttttgt
      841 agaagctatc aattgaatgt gatcaatggt agtcaacttt atggaccgta tctagggaaa
      901 ttctacggtt ttctagattc aggagatgag actggtagtg gaaaaacagc gccaggaatc
      961 catatcttaa gcggttcaaa ggtctctctt gattatgatc gggcagatgg cgttagcgat
     1021 gaaggagagg cagtcgatac gctacctggt aatgtcatgt ttgaagtcag tggttcggga
     1081 agcgaatttt cggtaaatac aaagatcaat attaccaatg acaacaatcg cggaatcatc
     1141 caaatgagag gttcaggatc gaaagtcact gtatcgaatg gtgggaaaat caccatcaat
     1201 acagcaacta cgggcggatt tcgcttgcaa ggagattcta gtcaaatcca tgtatcaagt
     1261 ggtggacaaa tctcggtcaa aatggctgga gatgacgatc aacccgggaa caatgggatg
     1321 cgttttgtag gaaaaggcct ttctttgatc gtagaaggag ccggatcagt tatcgacatc
     1381 gataagcaga gtgggcgttc cagcgcagtt cgttttgaga atggtacgca aaagctgaca
     1441 gtaaccaatg gaggctcatt gagggtaaga aatacaggtg atggcaaatc ttacgtgaac
     1501 agactcaatc aaggaaatca agcgatccaa ttttatgacg cttctggttt tctagaaagt
     1561 ccaggttcag ctgattttac agtcactgga gaaaaatcaa atatcgatat acgcgcagat
     1621 tccggagcaa ctgtagatac aactggagat attgatttaa attttacggc aggtgaaaaa
     1681 acttatttag tcatggtcgg gaaaacaggg gaagataaga ccggcatcat cagtggggat
     1741 gtgttgaatg tggacttaga atctcctacc tattttgatt tccaaaacaa acggattgga
     1801 agcgagacca atggcatagg cggctgggta ttccaaggaa atggagattc ttctttaaag
     1861 atcgcccatt cagaaatcgc tttatggcga aaaacaggcg caacattcga taactttgaa
     1921 aaagacccgg attatttttc tgatatgaca gacttatcga tcaatgggac cgacttaggc
     1981 aatttcgtat caagccctag tgcaggttta acaaatgaat ttgctcagac aggtgccgga
     2041 atgaaaaatt ataatcgaat cagtgccaat aatcagctcc cgatgattga atccttaaga
     2101 gtgccaacga atgcagataa aaaaatctat gggcatgtcg ttgttcccga aggtgtcgag
     2161 gcaaagccaa gagatgcttg gacaggcgaa gtagaagtga ctttacgtat tacttacgca
     2221 gatacgacga aatcatctgt agaaattact gctctcacgc aaggacaaac agatgaaaat
     2281 gaaggggcag gatataaccc atggggagaa ggcgaacaag gcggcttgtt cgaagttcaa
     2341 gtacccaatg atgagagatt aaaaacggag gatcaagtgc aagttgttgc tgctaaaaaa
     2401 gtaagaggtg gccaagaagt gacagaactg gactctgcca aaacgacagt cgatgtgatt
     2461 cctcctgaac ctgctaattt aagtaccaca ttgatcaatg cagcttctcg cacgatcaca
     2521 ggtacaggtg aagcaggagc cttggtttca ttgaaaaaaa atcaagctgt tttgtcagaa
     2581 acaactattg atgatacagg ggaatttgtt ctttctatcc cagaaaatca actttttgca
     2641 ggagatgtat tagaaattgt attaaacgac caagcaggag aagctactgc taagggtgtg
     2701 cagaacaagc caagtaccaa cgatgaaatt gggaatcaca atccaagtga tacgccagtt
     2761 gtttatcatg atgccccgcc ttttgcacct gctacgaaga tcgtagtaga aggtggtctc
     2821 tcatttcttg ccccttccaa actcgatttt ggtcagatca aagcaactgg tttgacacaa
     2881 gaagcatttg gccaaatgga tatacaggaa aaattatgga tttatgatca gcgagaaacg
     2941 aaaaatccgt ggatcttaac gataaaatta tctcagcctt tcacaaatga tgcaggattt
     3001 tctatgcagg atgtcctata tttcaagaaa ggagaggagt atcaattagt gaatgaagaa
     3061 gaactggaaa ttgtcagagg acaaaattca acgaatgtgc aagcagatta ttcagaatat
     3121 ttaaataacg gtcgggcgtt taaacttgtc ttaaccaaag aaaatcagat agctggtaat
     3181 tactcagcag agataacatg gacattagca gatgcgccga cagaataaag atcaaggagg
     3241 atgattgatg aatcgaatat ggaataaaag tcatcgaaac aactgctctt tacttatgat
     3301 ccttctcctt ggattcatcc tttggcagac acctgtccat gcctccgaat tcaattttgc
     3361 ggtagatttt gaattaccgg ctaatcagat tgataagaac aaaacatata taaatgtaag
     3421 gatggagcct gggaaaacac aggaaatccc ctacacggtc tttaaccgaa cagataaatc
     3481 agtaacagtt gagacattga tccactccgc tacgacgaat tcaaacggtg tgatcgaata
     3541 tggagaatca aaaagtaaat ttgatgaaac attaagttat gatatgaaaa acttagcaaa
     3601 aacagtggga gaaataactg ttcctcctca aagtgcggtc actgagacac tttcactgaa
     3661 agcgccagaa aaagagctgg ctggtgtggt cgcaggaggg atcacgtttc aagaaaaaaa
     3721 agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat cagttttctc aagtgaaagc
     3781 aatcttgatt tgaaatagcg aaaaagagtc ttccccagat ttgaaattaa aaaaagctta
     3841 tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat cttcaaaatc caaatgcttc
     3901 ttatatgttc ggcttgacta catcggttga aatctacaaa aaagaaacaa aagagcctta
     3961 cctttcaata aaaaaagaaa aaatagatgt agcacccaat acaaattttg atttacttgt
     4021 cccattagaa ggaaaaaaat tagctccggg acattatcaa gcggttgtga agggtgcttg
     4081 gaaagaaaaa cgctggaatc tccataccga ttttgaaata actgatgatg cagcggacca
     4141 attgaatgaa aaggatattg atttaaaaaa tcagccagaa acatttgact ggcgatggct
     4201 gatacttgtt ggagtcattc ttattggctt atttgctgga ttatttattt taaaagaaag
     4261 aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg ttctagctgg aagtctatta
     4321 ttatgtacac aaagtgtttt tgctgagaat aagagctcag ccactacaga aggaacgatt
     4381 tctgtgacca aaccagatgc tgcaaatcct gaagacgttg ttccagttcc tcctgttgat
     4441 ccaaatgatc ctgatactcc ttttacacct gacgaaccaa atcagggaac atccggatta
     4501 ctgacgattg accaagctcc agatcttgat tttggaacaa tcaagttagg tacagcaaaa
     4561 acagtatatg ctgcattaca aagcggaacg gacagtactg gagcacaaac acaagtacag
     4621 aatttcgtac aagtaacgga taaaagcgga aattatgccg gttggaaatt atcagtcaaa
     4681 cgaacagaat ttattggtac aacatctgat acgaataaac taactggttc aaaggtgaaa
     4741 ttcaaaaacg ccgtgattcg aaatagcgca actaatagca gtggaatgcc atctaccgtt
     4801 tctggaactg gcgctgaagg aatcgaaatc ccagcagatc aggcagtaga tctagtaact
     4861 gcaggagcaa atgaagggat tggcacatgg atttattcat taggagagaa tgtggaacaa
     4921 gggagacaat cagtggaatt atttgttcct gtatccaatt atgctgtaga caactacgtt
     4981 tcaacattca catggtcgtt aacagatgct cctacagaag aggaagcgta aaatcatatc
     5041 aacgatttca gggagtggag ccgattcaag gcccactccc tgaaatctgc tatgcttacg
     5101 tctcagctct tattcatcaa ctcgaagcag ttttttggaa gtcatggaaa cgttcctttg
     5161 tcttttcaaa gatctttcga ccaacgagta aggagtcacc gttgaaaaat tctaacacat
     5221 aatgagcaga atcaattttc tgcaattgcg ataagttgat acagtaagat tttaaaagta
     5281 aaaagtaagc atacttttct aactctgtct tcaattgttt taaagaagta ttcactaatt
     5341 tatcttctgt agttgttttg accataactt ggtttttcaa acctttgata ctttcaatat
     5401 ataaaatttc ccgaggatac aggacgagac ttccaccttt ttgatcaata aagattttat
     5461 cgacatttac gatctgctgt tctttccaat tgatctgttc tagcacttga cgaatttgtt
     5521 cagataaatt cctgtctttt ctgatatatc caatcggaag gattttccga ttgatgatct
     5581 ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt actatgtgca ttttttttac
     5641 gtatttgttc ggcgacatca atacctgtga gtgattgctt caagtcgata tcgataaaga
     5701 aaacatcgtg attatcaata gtgcccaact ggatcaactg attgatttga gagatagaag
     5761 tgagctgcac gatattctcg atatgatgat tgaccaattc tctgatgatc cattcgctgt
     5821 gaaactggtt atcttctatg ataaaatact ttttcatatg caggctccta ttaaaaaaat
     5881 atgtgtacat tttgaaacaa aacaatggag gaataagatg gtcgcaacag tgacacttta
     5941 tttgaccttt actttgattt tagatttagg tatctattta ttcttttcga aacaactgct
     6001 agtaaaaaac agctttggtt gtatagcttt gatggtggtg acacttttac ttcctatccc
     6061 taaagtgagc tttccttacc tagttggctt attattgatg tttctttttt attttactac
     6121 gaaaaacctg tttatcagct gtttgatcag taatcttttt tttgctatca ctggactcag
     6181 cttttttctg gtttttgatc tgatggatta tttaggaaag agacatcttg gaacagcaag
     6241 tttttttgca ctacttatcg catttatttt ctatctatta gtaaaaagta tagacaaaca
     6301 gacgagattt ctcaacttgc tttttactta tccaactgaa cactattttg gaggtatttg
     6361 tattttatta agctggggcg tgatttactt ggtattggga agtgttcact tccgctcatc
     6421 taattatctg cttctatctc ttttgtcgat agtcatcagt tttttttctt tgtttttttc
     6481 cattatgttg atcagtttcc aagtaaaaga aagacaacat caggaatcgc ttcaactgta
     6541 tcaaataaat gaagaatatt accatcatct agaggaattc aaacatgatt ataaaagtct
     6601 actgttttca ttaaaaatta ctcttcagga aaataaaaaa gaaacgttgg aatttttaga
     6661 aatattagaa gatcattctg cagctatttt tgaaaaaccg caaatacaac agctaacaaa
     6721 tattgtatcc ccagctgtca ggggtgtctt tttaaaattt ctagaaaaag cagaatcgga
     6781 gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa acaatcccga ttgatctggt
     6841 tgtttttatt cgctgtttat ctatttttat ttctaacgca ttcgatcatc gtgtagccga
     6901 tcagagtccg attacgatca atctgctgga agaaaaagag ggatcacgtt ttagtatagc
     6961 gaacaaggca aatccaacga atttgacgct atcggagatg gtcaaaagag gaaaaacctc
     7021 taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa atgttggatg tttacgaaaa
     7081 tgcagaacta aaaatcgaat tttcaaaaca gaatcactat ttttttgtag aaatgtatct
     7141 ggcatctaga aagagcaaat cagcctactg acttactgtc aaaggataag tttgaggaaa
     7201 gtctacacgg agatagagac aacaacacag catattcaaa agaaacagaa gcgtgatgac
     7261 cgttgtcacc cttctgtttc ttttgctatg tagttaaaca aagtagttga cacttccgct
     7321 acttagtatt agtacgaaag tatacttcat gatactaagt agataagaag tgaaaagcgg
     7381 aacccacgat cgtcaaaatg ctaactgctt tgcttacacc agataaaggg aagagcaata
     7441 tcgcttattt taggcgtgga gaagcaagca aatccctacg atcacaaaaa aaatgctgac
     7501 ccagcagtta agagagttag aacaagatga aagtatccat cgaaaagcct ataaccaagt
     7561 cccgcctaaa gtagagtact cgctcaccga agaaggaaaa tcattaagag agattctagt
     7621 agcgttgtct gtttgggaag aagaacgtat cgaaaaacaa aaagcctcaa gggaagaaga
     7681 cacgctactg catacacatg gtggctacct aaattattga ttttctatga catttatttt
     7741 aaataaaaga aaacaggagt ttttttaaaa gcatcatcta ttttttatgt aaaaaaatgg
     7801 tatgattaat caagtaagaa gtgattggaa gaaggcaagc aaatgaatag tatggaaaaa
     7861 gtcaaccagt ttagagatga acgtaattgg cgacaatttc ataacgaaaa agatttggct
     7921 atctctattt ctttggaagc atctgaattg ttagaacttt ttcaatggaa aacgccggaa
     7981 gaagtaaaag aaacacaatt agaacgaatc aaagaagaac tggcagatgt attgatctat
     8041 tcatacatga tggcagacaa ccttcacctg gatctagatg agatcattga agaaaaattg
     8101 atcaaaaata atttgaaata tcctgtagaa aaaagcgcag gtgtacgaaa aaaatacaca
     8161 gagttgtaga aatgtgtcat tcggctgttg gaaagagcaa aagaaaaaca gtaaaatgag
     8221 tctggaggga gagaaaatga aaaaaaaagg aaaagcaaaa agatggctag tcaatatcct
     8281 cttgttcctc ttgttattag taggactagc tttgattttc aatgaacaga tcaaggatta
     8341 ttttgtcaga gagacaggag ataaatatgc tatagcgaat gtaacaaaag aagatctgaa
     8401 aaagaataac gataaagatg tcagttttga ctttgatgcg gtagaaccaa tgacgacaga
     8461 aggggtcatg aggtcgcaaa tgagaggaac agatctacca gtgattgcta gtatcgcagt
     8521 cccttcagtt tcaatcaatt taccggtttt caaaggcttg gataatacgt cactacttta
     8581 cggagcagga acattatctc cagatcaaga aatgggaaaa ggaaattatg ccttagcaag
     8641 ccatcgtgca acgaaccctg aattgctgtt tacaccgcta gaaaatttag agatgggtgc
     8701 caagatctat ttgacagatt tagaaaatgt gtatacatac aaaacatttt ttaaagaaaa
     8761 agttgctcct acagataccc aattactgaa tgaagtcgag ggaaaagaaa ttgtcacact
     8821 aattacttgt ggcgacatgg acgccgtcac gcgactagtt gtacaaggag aactggaaag
     8881 tgttacatcg ataaaagatg cgacagatga tatgagatct gctttcaatc tagagacaaa
     8941 aacattttag gatactggga cataagtcgt tacagattaa aaacagccaa aggatatgaa
     9001 tcgataagga aaaaccaatt tgctgaaaaa aagagaaacg caaagaggtg catctctgat
     9061 ttggttgttt tctagataga cttgtgttcc agttttttta ttttttatat gaaaaggctt
     9121 gcataaataa aaattcgatg gtatgatgat tttgaataag aaatggattg ttactgtacg
     9181 gcaggcaaaa ccagaattca gctaagaaac agtaggcgaa ttctaggttt ttttattttt
     9241 cttgagaggg cgaagcaaaa tgaaaattga taaaattttg aataacaatg tcgtcatttc
     9301 gaaaaatgga ttcggggaag aagtcgtttg tatgggaaaa ggactagctt ttcaaaaaaa
     9361 gattggggac gagatttcgc cagaagcggt ccaaaaagaa tttgttttaa gagattcttt
     9421 tgctaaaaac caattccagc agctgatggc tgatattccg gcagaggaga tcgaatgggt
     9481 caaacaagta gtcgaactgg ctgaagataa actaaaagtt gaattttcgc ctaatattta
     9541 tctgacattg accgatcatc ttcattatgc gatcacacga gcaaaagaaa atatccaact
     9601 gcctaatcca ttattatttg aaacaaaaaa attttatccg aaagaatatc aagcagcgaa
     9661 agaggctctt ttgttcgtga aacaaaagac gggtgtggaa ctttctgagg atgaagccgg
     9721 ttttattgcc tttcattttg tgaatagcca acaaggaaac gaagacatgc aagtgacgat
     9781 gaccgctacc acaatggtaa gagatatact gagtatcatc agtaaattct tcggcatcgt
     9841 gttcgatgaa gaatctttga attatcagcg tatcattact catctgcagt tttttgccca
     9901 gcgttatctc aaaggggaag gatctgacga gcaagatgaa tttctctacg cattagttca
     9961 aggaaagtat ccaaaagcgt ttcattgtgt cgaacggatc aatgagtatc ttcgacaaac
    10021 gcaaaaacag gaaatgggga tcgcagagca gatttattta acgattcata ttcaaagagt
    10081 agtaagtgag aaaaaaagca tccaatcata aggattgtta ctgtatggca ggcaaaacct
    10141 ggattgaaaa aataggttca cgaatgtttg tgactgtttt tttcaatctg ggtttttatt
    10201 ttttaaataa aaaagaaaga aggaaaaaac aatggataat caagcagttg gtcgtcgtgt
    10261 atgggaagcg gtaggaggac aaaagaatgt caaaagttta gtacattgtg ctacgcggtt
    10321 gcgattcaga ttaaaagatg aatctttagc agatacgcaa aagttaaaag aagacccgga
    10381 cgtgatccaa gtcgtacaaa gtggcggtca gtatcaagta gtgattggca gtaatgtagc
    10441 agatgtttat caatcgatcg tggatgagga aggtcttgca gtttcggagg atggaaaaga
    10501 agagtccaaa aatattttga atcgtctgat cgatattatc tctagtatat ttacaccttt
    10561 tctaggggcg atggcagccg ccggggtatt gaaaggattc ttatcattgg caacggtgat
    10621 gggctggctg acagctgata gcggtactta tcaaatattg tttgctgcag cagatggtgt
    10681 ctttaccttc ttacctgtca tgcttgcttt tacagcagct aaaaagttca aagccaacca
    10741 gtttttagca gtagcaattg ctatggcact tgtgtatcca gctatcacag cagttgctgg
    10801 tgcgggagaa gctatcagtt tctttgggat cccagtgatt ctttcaccat ctggttatac
    10861 ttcttctgtc ataccaatca ttctagccgt atgggttcaa agtaaattag agccattcgt
    10921 aaaaaaagtg attccgcaat ttctgcaaat gattttagtt ccgcttgtcg ttttagtagt
    10981 gatggttcca ttaacatttc ttgctttagg accaatcggg acggttgccg gtaatgcttt
    11041 aggcggtctc ttcaactcga tttatggatt tagtccaatc gtagcaggtc taatcatggg
    11101 aagtttatgg caagtattcg tgatgttcgg catgcattgg ggattcgtac cgatcatgtt
    11161 tttgaatatc gaacaatatg ggtttgatgt attgatgcca atgcttcttc cagccatctt
    11221 ggcacaaggt ggggctgctt tagctgtagc attgcgaaca aaagatacga aacttcgagc
    11281 attagggatt tcttctacag tgacttcgtt atttggaatc actgaaccaa ctgtgtatgg
    11341 tgtgacatta cctttgaaga aaccattcat tgccgcttgt atttctggag gtattggtgg
    11401 agctattatc ggattttccg gagtaaaagc tttctccagc agtttggtca gcttattgac
    11461 gattccgact ttcattaata cggtggatgg tgtagagtca aatgtgacag tagcagtaat
    11521 agctacagga attgcttttg ttcttgcatt cgtaggtaca ttgattttag gatttgacga
    11581 acaaacacaa gacaatcaat tagaaaacaa acatgcgaat gcaggaaaac cgattacttc
    11641 agcacggcat accttaaaaa gtcctttaac gggtaaagta ctgccattga gtgaagtacc
    11701 agaccaagtt ttttcttctg gcgtgatggg aaaaggtatc gctattgatc cagaagtagg
    11761 agaactagtt gcacctgcag acggagagat cacgactatt tttccaactg gacatgctgt
    11821 agggatcaca acgactgacg gagcggagat tttgattcat atcgggatgg atacagtaga
    11881 attaaatggg aatggctttg agatcttagt caaacaaggg gatctcgtca aagcaggaga
    11941 tttattgatt cgttttgaca tcgaagcaat tagagcagct ggctatagcg taatcactcc
    12001 agttgtcatt acgaatactg atgcatttgc cgatatactt gaattagacc aaaaagagat
    12061 tattgcaaac gaagatgttc tggcaattgt aaaataagga ggaaatcagg atgcaatcac
    12121 gtttttcaga aaaattttta tggggtggag caactgctgc caaatcaatt agaaggggct
    12181 tatgatcgag acggaaaagg actatccgtt gcagatgcta tgcctggagg taagcagcgc
    12241 tttgcgatta tcggcagtga agaatttgac tggacgattg atcaagaaaa atatatttac
    12301 ccgaatcatc gaggaatcga tcattacgat cgtttcaaag aagatcttgc cttgtttgcg
    12361 gaaatgggat tcaaatgtta tcgtttttcg attgcctgga cacgaatttt tccaaatgga
    12421 gacgaaggta ctcctaacga agcgggatta gaattttatg atcagctgat tgatgagtgc
    12481 ttaaaatatg atattgaacc tgtgatcacg atttctcatt acgaaatgcc tcttcatttg
    12541 gcaaaagaat acggtggctg gaaaaatcga aaactgatcg acttctatga acgatttgct
    12601 cagacagtat tagagagata cagcagtaaa gtcaaatatt ggatgacttt caatgaaatc
    12661 aattcggcct ttcactttcc agctttaagt caaggattag taaaaagcaa tggagcagga
    12721 gagtaccaaa atattttcca agcatggcat aatcaattcg ttgcaagtag caaagccgta
    12781 aaaatcggcc acgaattgcg ttcggatatc caaatcggct gcatgattat ctatgcaaca
    12841 acttacagca tcgatgccaa tccagtcaat caggcagcaa caatgatcca aaaccaagaa
    12901 ttcaattttt tctgcacgga tgtccaagta cgtggagaat accctgcata cacagcgcgt
    12961 acccataaaa aatatggagt cgatccggaa aaattagagc aaacggaaaa agacttccag
    13021 ttgctaaaag aatatccagt ggactacatc ggatttagtt attatatgtc tacggcaatc
    13081 aatgagactg atccacaagc agcaacttca gaagggaact tgctgggagg agtaaaaaat
    13141 ccgtttcttg aggcaagtga atggggctgg cagatcgatc cagaagggct gagaatcgca
    13201 ttgaatgaat tatacaatcg ttaccagaaa ccattattca ttgttgaaaa cggtcttgga
    13261 gccattgatc atatggaaga ggatggcacg attattgatg attatcggat cgattattta
    13321 cgtcgtcata tcgaagcgat ggctaatgca gtagcagacg gtgtagattt gatgggctat
    13381 acgccttggg gatgtatcga tcttgtcagt gcatcaacag gagaaatgag taaacgttac
    13441 ggctttatct atgtggatct agacgatgaa ggaaatggga cactgaatcg atcaaagaaa
    13501 aaatcttttg attggtataa agaagtaatt gcctcaaatg gcgtaaactt gtaattaaag
    13561 cagctgaatg aaaaagaaca cacttctcac atgtgatttg tgttcttttt ttatttttta
    13621 tgtgctattc ttttagtaat agtaaagcaa agggtgacgt tatgaaaagg acacgtgtac
    13681 tatacttgga agtcgcagat aaaattaaag aagatatttt ctcaggaaaa tatccagtcg
    13741 gttcgatgtt gccgacagaa agtgaattag aagaactgtt tcaagtcagc aaaatcactg
    13801 tccgaaaggc aatcgaactg ctggcatcag atgaatatgt cgcaaagaaa agtggtcgtg
    13861 ggacgacggt tctaagcaat cgtccttaca atcgattatc caaagcggca agctttacgc
    13921 aaatcttgga gaactcccga tatgaagtcc ggaaagaaac gctggatttg caattggtcc
    13981 atataaagaa ggaagatcct ttgttttcct ttttcggtga gcaagctgtt tgttttaagc
    14041 gcttatatta tttgaacaat cagccttata tttattttgt tcattatctt ccacaagaaa
    14101 tgatgcaatt cagcagagaa gaatttggca aagaatcttt gtatcgtttg ctgacgaaaa
    14161 aaggatatac tattgactca ttttctgatg atttctcggc agtattttta acagaagaag
    14221 aacaaggaat tctgaaaaca gacgcacaga tcggcatcaa acggatacgg aaatcgtcta
    14281 cgcaaattgg ggaaatgatt gaatattcgg aggcggtcta ccatacggag ctgcatcctt
    14341 accatattga ttatgaaaca taaataaaaa aagaggttgt gaaaaagctg ccctgcatca
    14401 agtaataaga acagccaaac aaaaatagct tctcatattt ttcgtttggt taggcttatt
    14461 accgcagata cacggctgtt tctggattac catttattct ggataagggg gtgtggcacg
    14521 attttcagaa ggaatctatg aaccaatcac tgtattgatt ttagcttcga tggtaacccc
    14581 attcggcttg acgatcgctt atttcttagg gaaaatcatt cgtaaaaata tactcaatcg
    14641 tcaagaaatc gatacattga agaccgcttt tcctatgggg atctgtcaga tcacagaggg
    14701 atgtttccct atcgtattga acgacttggc acgcaatgtc atagcaacgg gagtcggtgg
    14761 gacagtaggt ggcggattaa gcatgttttg gggcgcagat agccgtattc cggcatcggg
    14821 aatgtttgca gtcgctacga tgaccagacc atgggcattc attggtgcat tgttagctgg
    14881 ttcttttgtc acaggaataa ccattttatt attgaaaaaa ccagtagatc cgaatgcaga
    14941 aatcatccaa gaagaaaaag aggaagaaga catatcttgg gatgatttga caattagtta
    15001 ggagaggcag aatggattac aagaaaaaag tacaatagat gtaccttgag acaggaatgg
    15061 tatgaaagaa tcagagttag aaagaatcga atatacagat tttggtttag gagcgatcga
    15121 aactgtccag agcatcggca ccctccttga aatgacgatg aaggaaaaat gagaaccttt
    15181 cgttgtcgaa agggtctggt ataccttatg tcgaaggaaa ggaaacaaaa aaactttcct
    15241 gtactattcc aaaaaggaaa gaaagttatt atacagtaaa aaaagaaatt attcttcatg
    15301 ctagagatca gtacacgttc gagcccaata tcaaacacta gttccaggca ggcgcaacag
    15361 gtgcggttat cactgaattt tcttccagca gtgacgattc aagtgatatt tttacaaatc
    15421 cagctattta tcgttaacaa aaagattttt aggagaagct gatgaacaaa aatgatcaaa
    15481 ttttgatcgt ttagttcgtc ggctttttta tttagagaaa catcatttat cagtgaaaca
    15541 taacacgaga acaaaatgat tt
//
LOCUS       DAZULY010000047        15445 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000047 DAZULY010000000
VERSION     DAZULY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.047"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..1384)
                     /locus_tag="ACG81A_000974"
     CDS             complement(20..1384)
                     /locus_tag="ACG81A_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS5014360.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1381..2940)
                     /locus_tag="ACG81A_000975"
     CDS             complement(1381..2940)
                     /locus_tag="ACG81A_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5014361.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(2996..5476)
                     /locus_tag="ACG81A_000976"
     CDS             complement(2996..5476)
                     /locus_tag="ACG81A_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014362.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(5460..5795)
                     /locus_tag="ACG81A_000977"
     CDS             complement(5460..5795)
                     /locus_tag="ACG81A_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014363.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5809..6843)
                     /locus_tag="ACG81A_000978"
     CDS             complement(5809..6843)
                     /locus_tag="ACG81A_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS5014364.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(6933..7613)
                     /locus_tag="ACG81A_000979"
     CDS             complement(6933..7613)
                     /locus_tag="ACG81A_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS5014365.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(7897..9000)
                     /locus_tag="ACG81A_000980"
     CDS             complement(7897..9000)
                     /locus_tag="ACG81A_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFS5014366.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(9629..10099)
                     /locus_tag="ACG81A_000981"
     CDS             complement(9629..10099)
                     /locus_tag="ACG81A_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFS5014367.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            10429..12069
                     /locus_tag="ACG81A_000982"
     CDS             10429..12069
                     /locus_tag="ACG81A_000982"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFS5014368.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            12223..13545
                     /locus_tag="ACG81A_000983"
     CDS             12223..13545
                     /locus_tag="ACG81A_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFS5014369.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(13613..14047)
                     /locus_tag="ACG81A_000984"
     CDS             complement(13613..14047)
                     /locus_tag="ACG81A_000984"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5014370.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(14112..14949)
                     /locus_tag="ACG81A_000985"
                     /pseudo
     CDS             complement(14112..14949)
                     /locus_tag="ACG81A_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
BASE COUNT         4512 a         3116 c         2798 g         5019 t
ORIGIN      
        1 taaaagaccg tgatatttct cacagtcttt tatctactct attgctattt gctctcttag
       61 ctagtacttt aaaagctaac tcattgaggt agtgaaaacg agaggtgtgt tcagacagaa
      121 atccaaaatc ctcaactgct agctgtggaa tcgtagtttt gtgtttcacg atatccgcaa
      181 gcaggttcaa atctgataat aagaattctt ggcttatcag ctgtccgcca attatttcta
      241 atgtatccgg atggtagatc agctttgcat ttactgtttt gttttcttca tcgtattgtg
      301 gaaatagatc aaattctcct gaacacgaac cggtattttg ttccaagaat ggcgcttcat
      361 cttctgtcag ccctacagtt cctaaaaagt agtctataat gactgctgct gttatctttt
      421 gtctgaggtc gtaagcggtt ggctgaccac tcaaagttag cgctactgct cttgccatat
      481 gtacagctcg agtaactaaa ggtaaaaaga gatgccgttt atttttgtca aaactgactg
      541 ggacgatatc tccaatcgca tagatgtccg gatcacttgt ctgcagatat tcattgacta
      601 tcacagtctc atcctcatac aaagccactt tttcttttaa caatgtcgta ttaggacgag
      661 tttgggcaga aaaaatgacc atatctgcct ttattgcttg agatagaagt tgtacggata
      721 ctactttctc ttctgatttt ttccaatcga tcaagaattc atttggataa aaattgattt
      781 gcttagtttg cagctgattt tgaaaactgt tgatcatatc cttatcgaaa tatcttgata
      841 aaacattcgg catataatca actagatgaa taatgtatcc tttatttctc aaagcatctg
      901 ctaattccaa tccaatataa ccagcaccga agatcactac ttcttttgct tcttttaaag
      961 cggcaagtac ttgcttagat tgcactagat ttttataggt gtagacattt tcagcttcaa
     1021 tttgtttata caaggtgaca ggagaggaac ctgttgctac gattaattta tcataggtta
     1081 tggtctcgtt ttttccttct ttggatgcga ggatgagctg atgtttgtct ggatcaagtt
     1141 cagttactct ccattcggtg atcagttttg ccccactttc ttgaagagtc cgcatgttat
     1201 gggcaacttc agctaaacta cgtatctttc cttttaaata aaggttgatt ccatttgaaa
     1261 catagcctag tccatcttta tgatcttcat cgatcagtaa aacttctgct ttcggttgaa
     1321 gttttcttag atttagggca gctgtgatgc ctgcatggga agcaccaatg atcacgattt
     1381 tcatgtttta ttcatcctta aattcaattt ttctaaataa cattctttta aaaattggtt
     1441 caaatagggg aaatcataat tatattcgtt gctagtgaat acaaaaactt tagcggactt
     1501 ctgtgacggg tactctttca attggctagt caaaatgata tcatactgct tttctttctc
     1561 gtattcgact aaatcatatt tgatcgtatt cagcaattgt tcctggatga tttctttcac
     1621 gatatccgcc attagccttt cataaggaag atcataagca atttgcagac gtaattctaa
     1681 ctgcttgatc agcgtccgca aaatagatgc atagcgagca aataattcaa agcgggcatt
     1741 ttcctcgtct acttcagtaa aatcaacaat agcaaatgtt tgtctgacca atctttcaca
     1801 taaaaccagg aattgtttat cttctacttc agttaaccgc tgttttaatc cttcttggcc
     1861 aaaaatattc atccatcctc taaaataggc aaaatgagcg tgattttgca tcactgtcaa
     1921 cgcaatcttt tgcgaaatct cttctggaaa aacataaaaa gagaaatatt cttctatcga
     1981 ttgtttaaac gttcgattca attgagctac tacgtcgtct ttgtattttt cgcttatcaa
     2041 aaactcctgg acatgcgagt tatgtgtatc taatgtaaag ttactgatca tgaaaagata
     2101 aaaaaccaac gtttcatttt gattccaagg aaaggcatat tgaaagaagt aacgggtcaa
     2161 aaatgtatgg aggtccaata aaaattgtgt cgtttgaaac aatccaacag gaagattgct
     2221 ataatctggt ttggattttt tcgtacagcg cattcgcttc ttcataatgg aaagccatat
     2281 ttcaaatttc aatcgtcctt cttctgtaat agtaaaacta aactcttttt gcagcagatc
     2341 taaaaaagtg tttcctgata aatcactgta ttcttttttg ttttcaagat aaggacgtcc
     2401 aaaccaaaat aattggaaat aaaaataaca aatttgactt tctggaccgg tcagccgccc
     2461 gctttttatt ttcagatgaa actcatttaa agaatcattt aattccttca ctctgcgaga
     2521 caaagtagcc gaactgatat taagttccat gacaagtttc tgccgatcaa atgagccatt
     2581 ttccaacaga taaagaagta tctgatattt tgtagaatag cgcaagtagc aatgataata
     2641 aacatcgact gctgctatat ttccatgtct gattaataaa atattccctt cttggataac
     2701 aatttccaat tgtttgatat tttccaagtt agcttgtaaa taaaacagat cttctttcaa
     2761 ggtagcccga tcaactgata agcgggtaag tacgttatta acggaaatct ctcctttttc
     2821 ctctaataaa agttggaaaa gtttgatttg ccgttgttca tttttttcaa gcaaactgaa
     2881 aagttccaag tccactcctc caagcatttc tgattttaac agcagaagtt tccttctcat
     2941 ccttgaaaaa gaggatctgt gacagttatc acagatccat acaaattcta atcacttagc
     3001 tggttggccc agctgtcagt tcccatgtga ccatgccttg atacgttgcg ccaggttgag
     3061 cattcgtact gctatttacc tctaagagta aaccatcggt atcgggaaag tcaacgagaa
     3121 cctctccttt agctgttcca tcactctcat aaatcaacgt gttttgacct tgctgaatca
     3181 attgttttga tccagaatga ttgaagtata attttgttcc gcttaattct tgaccttgat
     3241 cattcttaaa taaagaagtc aatgtccctg tcagttgcca aggagacgtg cttggaagcc
     3301 gagtatcccg cacatcaaaa gaaaaatcgc cttttgcttc ataataactt gtcttcaacg
     3361 gtttggggag atcgttacca aatgtaaaat catctggtac attaactaat gttaattctt
     3421 gtttggggac aagaattgtt ttagcagatg tttggacaac tgggttagga agcttagcac
     3481 catctgctaa tgtaatattg ctggaaagta cagctgcctt tgtcagtatg ctgacggatt
     3541 gtacagaagt atctacagta tttaattgat agttgtaagt aagatgaatg gtatctccta
     3601 ttttgctcaa tagatctgct gggacagaaa ctccttgttg aatgccggta tctgcgatag
     3661 ttgtaccatt taattttaag gaaccattga tataagtagt attttcagga agtgagatcg
     3721 tgattttttt gtcggttatc gcatccccag attttgttaa cgtgaatgtc acattttctt
     3781 gaatcgtatc tcctgataga gcagacaggg gatctcctgt ctcaggttgt ctgctaaggt
     3841 tttttagctg actatctgca ctcacagatg gaaaattagc cactgttatt tgaggaagag
     3901 tggtgattcc agcttgttca gactgccatt tgtacgcaaa agacggatac atgttagtaa
     3961 gtacatcgtc caccaagcga taatcaaaat aatattgccc tgaagccaat gcaggtagtg
     4021 tagaagtagc tgtatttaca cccccgttat ttgattggat cgtttggaaa agttgatctg
     4081 tccatcctgt cgctaatttt tgggttcctt tcgaataaag cctgtaatgg atcgaaccct
     4141 tttttagttc agttgctgac caattggccg tgacttggta cgatgcagaa gaaaggagct
     4201 ttccccactg actatcggct gttaagtttg ttggtaaaac cgttccgtct agcttgtact
     4261 gctccaatac ctttaaagca acaggagaaa gcaatccgcc ttgcgcatct ttcccactgt
     4321 acacgatatt ggctgtctga ttcaatccac ttgtgcttgc tggagtatga gtcagtattg
     4381 ctccttgtgt aacgtttttt cctgtaaaat agttgactac gcgatcaaag cgactaaatg
     4441 gcaactgatc aaaacgtcct tcagtcagcc ccatcgtcaa tgtccgacta ttggtagagc
     4501 ctggaaggtt ctggttattt ccatccacgt attctgtggt atcagcaaat gtattatatt
     4561 ttcctgttga atcacttgtt gagatgtaac tattccacgt atttatctct gcacttgcag
     4621 aaaatgcata tgctcctgga ttcaagtttc cgccagttaa tttaggataa aatctacctg
     4681 cagctggagc taaaatcgaa ttgaacatac gatcttggat aatattattc gaatcataaa
     4741 aatcaacaga aaaaacggcc tttttagaca tagaaggttg taatttaccc ttggtcaaaa
     4801 ttttgtttgg actcgtatca tatttatctc cgttttctgt catcttcgcg acattagctg
     4861 taacatcaaa ttgaagcggg atatcgtcca cgttgaattc gcctcgcgta ataatagcaa
     4921 taatatctgc caatagagta tttgtatccc acgaccccat cccaaaacgc aaggtatggc
     4981 ttcctgctga taataaacta ccgctagtaa ccttgatggg ggcaccggca atatacaagt
     5041 aggcgttgtt ccagtcaatt gcttttaata catcactgct tgatacatgt tccggcaaat
     5101 tgatgatcgc cacaaatgca aatccttgtg cagtgatatt caccaaatca agtacatttg
     5161 ttagtttcac atggctttcc caatgaaaac ggaccgtgcc acctttccaa ggatcatttg
     5221 aaccatcgtt caaataagta gcagtcaatt taggcaattg gttagcccca gttagttgga
     5281 caagcttccc gccattgact gcactacgtc caccatttgg aggtacgata ttttcatacg
     5341 tgtatggatc ataagaaatg gcaggaggcg ttgctccaac agcttctgcc gaaggtgaaa
     5401 agataaatat acttaaaaca aaaagcgaag taaaaaagaa ggagcgaaaa aatgaaaatt
     5461 tattttgtag caacatgttc atcctccttt tcctttcttc tccataacaa caaccacgca
     5521 agaagccata attctatacc aatgatcaaa taaagaaaac ggatcttttc ttcattcgtc
     5581 tccgggaata cgccatgttg attagattct ttataaatcg gtagctgtgt ctgctgtttt
     5641 gatgcgccat tgactgaacc acttggatgc ggaactggac ttagatcata aaatgtaatc
     5701 gtacctgaac ttttggtatc tgcttggact ggctcttgcc agctgaagaa cagcaaactt
     5761 gcaataatcc ctcccaataa gatcgatgtt ttcaacgaaa tcacctcatc atgttgtttc
     5821 tccttttttc tttttcttgt ggacccacaa aaacagcaaa atcaaccata acactagaac
     5881 tgcagcaacc aatcctgcat aaagccatgg gaaagattgt tcttgtactg caaaaacgga
     5941 attttcattt actttttgtg ctttttcttt tttgatcgta aagttttgtg taaatcgcca
     6001 ttcattactg tcgcctgatt ttgctatgat ttctgcgcgg taatcgccgg cttttgcttt
     6061 atcaccagct aaatcaagat tcaagtgaaa attcgaattt ggtgcaacta aaaattcatt
     6121 agacgtattt tcaatataaa gctgattttc ataaaagatc ttgctcgtaa atatcaaatt
     6181 gctgataatc ctaggctgag gattttggaa acgaagctga acagttggct ttgcgttgac
     6241 ctcgtccaaa tctcctgata aaagtttcag atccggttct atcgtctctt gactttcttg
     6301 taaaacgact gcgagattat aagcaatacg atttttgatt tgtgctcctt ctgtagcatt
     6361 ttctgtttca gcttcttctt taggtgaaac atgaataccc gctaatacac gtcccttaaa
     6421 gctttttgct gggattttca cttctatagg tatagttgtt tctgcatgtg ctgatacatt
     6481 gatttccttt ttcgcaatga gcaaaacgtc acggacatca aacggcaatg tctcatctaa
     6541 cgttgggttt ttttgactat aatcgatcgt tccaccatca ctcgttaccg ccggattgac
     6601 agagacctca aatgttcgtt cttcatctga agaattgctg ataattactt ctagtgtctg
     6661 attttgttcc ggtgccacta ataaatcaaa ataaccgatt gctttatcta tttggttctc
     6721 tggaaatacc ggtttgattc caaagtctcc tgctgaatcc tttgcttggc tgattgacgg
     6781 aaaaatgaaa aatagactta aaatagaaaa aaacgaaaca aacagtcgtt tactttgatt
     6841 cattgtccac cacatccttt caacaacaac tgcggtaaaa aatccttcac ctatagtatt
     6901 ttcaccgcag tttgttgaat ttatttcata ctctatgcta ctgggccagc cacgaggttc
     6961 caagtcaaat cagccgtgaa tgttccagct gctgctttcg gcactttcag tgtgatattt
     7021 gctggatcat aaacagaaag ccatgttccc agaccttgat cttttgctgc tgtaaatatt
     7081 ggtgtcgctg cacttgcttt actcaaccca gttacagttt gactagttgg tccagtggat
     7141 tcgttatctg acgtactttt aacagtggat gttccaaaat ctaattcagc gccttgcaaa
     7201 acttgtgaac cgtttacaaa atctgaaacc gaaactgtca cgttccaccc ttgtgcttgg
     7261 ccatctgcac ctacacctcg acgatctgat acttggagat aaggtgtatc gttttttgaa
     7321 gcgtctactt gatacgtttt tgttccgctt tcaatctcgt gtgtaccaaa tggcaattca
     7381 ggaactacat ccaatgttaa agaacctgtc tggccagttg ctggattttc cggatctgct
     7441 ggctctactg gttttgaagg atcagtaggg ttaagaggct caacggtatt tgttggttca
     7501 gtaaaggtaa tatctccttt tgaagtcgct tgaccctcag caaaagctgg tgttgccaag
     7561 ccgcctagaa gaagtgttga aagtaataat gtcccaatca tgttcttttt cataataaat
     7621 cactcctttt tcttagtgac ctaagtatag tagagacagt atgcaaaaaa tcctcatgaa
     7681 ttcatccgct tacttgaaat tatgaccctt tttgtataaa agagaaaaaa acaataaaat
     7741 cgtttacatg aatattcttc cttttttatt aagtatgaaa aaatatttca attttatttt
     7801 ttcgtttcaa attattctat tagtgacatc cttgaactat aaaaatagga gtacggcatc
     7861 atctgccgca ctcctatttt tttcgtcatt tatttcttaa ataaaacgat tctcacgcaa
     7921 ttttattttt ttgatatctt ctactgtttg tgttccagct aattgcatga ccatttccaa
     7981 ttctgtttta aagaaatcaa acacttgatg gactcctgta cttcctccta aagacaatcc
     8041 ataaataacg ggtctgccaa tagcaacaag atcggcacct gatgcgattg ctttgaaaac
     8101 atgttgtcca cgtcgtacac cgctgtcaaa cacgattggt acacgtttat ctactgcttc
     8161 tgccacatat tgcaacgaat caaacgcagc aggcccccca tctaattgac ggccaccatg
     8221 gttagatacc caaattccac cagcaccaga ttctaatgat cgatagacat cttcttctga
     8281 ttgaacacct ttcacgtaga ctggcaaatc agaatgagca gcgataaatt ctacatcttt
     8341 tggactaagt ttttgtttgg aagatttata tacagcatcc atcgtttggc ctactcctga
     8401 ttgataagcc tgaacgatcg gcattggcag cgggaaggta aacccgtttc tgcggtctgt
     8461 ttcacggttt ccgccaactg ttgcatccgc tgtcaatacg attgcttttg ctccattacg
     8521 cttggccact tcaagaatat ccaaattgat tccattatct ttgctcatat aaaattgaaa
     8581 ccattgcggt gctttctccc cgcctgcttc tcttatctct tctaaagtac aagaagcgta
     8641 agaacttgct gtatagatcg tcccaaaatc tgctacacct tttgcagaag ctttctccgc
     8701 cttcacatga gctaatccat gagcagcaac tggtgccatg atgattggtg cagtcagcat
     8761 ttcttcgtca aaatgtgtag ttgtatcggg taattctaca tctcttaata catgaggaat
     8821 aatcaaacga tgattgaatg cccgttcatt ttcttgataa gtaaataagt cgccagcacc
     8881 actgctgatg taaccataac caccagctgg aatcacttgt gaagccgcga tttctaaatc
     8941 ttccatattg ataaaatcta ctattccttc atttgtacct gcttgatatg ttttttccat
     9001 ctcaatgcct tctctctata aaatttgaat gatgaacaat atgatagcgt ttacactttt
     9061 tatattactc ttcgacattg atacaggcaa ataatctgtt tacatttcag ccattccaga
     9121 tgtcactact ctggaagaaa tgattgtaat aaaaatagat aaaaaaccga tgggactttg
     9181 acaaaagtag tgtcaaagtc cctttttttg aataaacggt gttcaaaagc cattttctcg
     9241 gtattaagtc aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt tttcttacta
     9301 ctcgaaacta agcgactttt tcacaacctc tcgataaaac ggtgttcaat cagctgaccc
     9361 tcggtattag ctcaaaaaat ggcaaaatat gaggaactat tttgccattt gtctctctaa
     9421 tactcaggtc agaacgttga cttcacagtc tcttccttaa acggtgttca aaaacttgca
     9481 tctgcggtaa taagcccaac caaacgaaaa ataggaggag ctatttttgt ttggctgttc
     9541 ttattacttg atgcagggca ggtttttcac gacctctgat tcacggtgtt tctttttcat
     9601 caacattttt acgaaatact gtggaagact attgaaataa gtgtggattg tttctttgtg
     9661 tttcaatcgt ttcttgccat tcttcattta attttggatt accgttttct tccttgaact
     9721 cataaggtcc taattcaaaa atcccagaca gtatttgatt cacgatttca tatagttcag
     9781 tgtgatcaga cacatgattc ttgaagatca tctcattatc accacgtttg aacctcacat
     9841 tcgggctctc atgatgaacc tctatgcatt tttctccttt ttttaactga cggacttctg
     9901 aaaacatctc ataaatatct tctccagtaa aaccttcagc aagcgccaga ctgtccacaa
     9961 gaccgcctac tgtcagttca gagattattg cttgattgga atctaatttt aatgtatagc
    10021 ttgtcgcttc tactacgcca catgcattgc aaccgccttc tgatagttcg ctttttctaa
    10081 tcaattgaac ttctttcatt ttctcttcct cctcttaatt ttctacttat gtatttttat
    10141 cacctgacat aggtgactcc tatcaacgct atcatgtgct tttttcctca gaaatcctat
    10201 ttatccattc catctatccc ttattcttct acttttcttt ctctagcata taagagatac
    10261 gcatagtatt caagcaagga ttgtcgatat aaaaagaaca aatactgcta ttttgatttc
    10321 ttttggctat attacatata aataaaattt atcatacctg ctctttttga ctatatcatc
    10381 atctgacttc tatacttcgt ttgtattaaa acttaggagg aatcattcat gcttacaggg
    10441 ttaaacttat taaataatcc ttttctaaat aaaggaacag cttttacaaa agaagaacgg
    10501 gcaaaatatg gaatcactgg tatgcttcca agtactgtcc aaacactgga acaacaatct
    10561 gttcaagctt atggtcaata cttaagcaaa caaacagatt tggaaaaacg tatcttctta
    10621 atgaatctat tcaatacgaa tcggacattg ttttataaat tgatgggcga acatttagtt
    10681 gaattcatgc ctgtcgttta tgatcctgtc gttgcggatt caatcgaaca atacaacgaa
    10741 attttcttga aaccgcaaga tgctgcattt ctttcaatcg acgaaccaga aaacatcaaa
    10801 gcaagtttga agaacgcggc agatggacga gacattcgtt tgattgttgt gactgatgca
    10861 gaaggaatct taggtatggg cgactggggt gtgaacggtg ttgacatcgc tatcggcaaa
    10921 ttgatggtct atactgctgc tgctggtatt aatccagcac aagttttgcc tgtatcgatc
    10981 gacgctggta ctaataatga agaattattg aataatccct tatacttagg caatcgtcat
    11041 gctcgtgtag aaggcgaaac ttactatgaa ttcatcgatc aattcgttca atcagctacc
    11101 gaattattcc cagaattgtt attgcattgg gaagattttg gacgaggaaa tgctgcagcg
    11161 atcttagaaa aatacgaaga tcaaatcact actttcaacg acgatatcca aggtacagga
    11221 attgttgtcc tagcaggtgt attaggcgga ttgaatatat ccggtgaaca attgaaagat
    11281 caaataattt tgacatttgg tgctggtact gctggtgtgg gtatcgcaaa catcctttta
    11341 gatgagatga tccgtcaagg tgtacctgaa gaagaagcac gccaacattt ctatcaagta
    11401 gataaacaag gtttattgtt tgaagataca gaaggattga ctcctggtca aatcccattc
    11461 gctcgcaaac gctcagattt cactaatgct gatgaattga caaacttaga agcagttgta
    11521 aaagcgatcc atccgacaat catgattggt acttctaccc aacctggagc attcactgaa
    11581 gaaatcgtga aagaaatggc tgctcataca ccacgtccga tcatcatgcc tttgtctaat
    11641 ccaacaaaat tggcagaagc aaaagcaaaa gatttaatag aatggacgga cggaaaagca
    11701 ttagttggaa caggtatccc tgctgctgat gttgaatata atggtgtaac ttaccaaatc
    11761 ggtcaagcaa ataatgcttt gatgtaccca ggtttaggac ttggtttgat tgcttcaaca
    11821 gctacacgag taaatgctga aatcatttct caagctagtc gagcattagg cggaatcgtt
    11881 gatgtaacaa aaccaggagc agctatcttg cctccagtag caaaaatcac tgaattttct
    11941 caaacgattg ctgaaactgt cgcaaaatca gttgtcgcac aaaacttgaa tcgtgaagaa
    12001 atcacggata tcaaagaagc agttgaatct gctaaatggg ttcctgaata taaatcttta
    12061 gaagattaag atcgttatag taaaagaaca tacagaaact ttgtatgacg tctaaaaaga
    12121 aaaaagcgac tgcggcaaaa cagcggccag ccaactcctg ctcttttgtc gcttttcttt
    12181 ggcttttttc atatgataca gaataagaaa ggatctgtaa aaatggcaca aaaaacaaca
    12241 actgcaactg cagttgagga aaagaaaaaa gaaggttttt ggtccacaaa aatcaccggc
    12301 gtaagtttgc cactttattt gatcatggtc cttgtcctga tcattgcaat ggctttaggg
    12361 aaattaccga cgaatatgat tggaccaatc gcagttttgg ttattttcgg taatttattt
    12421 cattttatcg ggacaaaaat ccctatcgtc aaaagttatt tgggtggtgg ttccgttttt
    12481 gcgatcttcg cttctgctgc gattgctaca tttgggatct tacctgaata tgtagtgaaa
    12541 tccacagaaa actttgtcag caatatgggc ttttgggact tttatattgc tgccttgatt
    12601 actggtagca tccttggtat gaaccgtaat ctattgatga aagcttctgt tcgttttatt
    12661 cccgttgcac ttatttcaat ggtcgtctcc ttttttgccg taggactcgt gggcatgctt
    12721 atcggaaatg gttttgcaaa ttccgtttta tacatctcat tacctgcgat ggctggtggt
    12781 gttggagccg gagccgttcc tttatcaacg atttatgcaa atgttttagg tacggatgct
    12841 tcttctatca tttcccgcct gattcctgcg acagcaatga caaatgtctt agctatcatc
    12901 ggtgcagcat tagttgctcg tgctggacaa tcaatgccaa aattcaacgg caatggtaaa
    12961 ttaatggaaa aaggcgatct tggcgatgga aaacctcgaa ctgtgaaacc ttccattcca
    13021 cagttaggtg ttggtttgat ggtatcattg actttcttca tcttaggaca aattggtaac
    13081 tattttgttc ctaaagtcca tgcttacgca tttatgatta ttatcgttgt catctgtaaa
    13141 atcgccaacc tattaccaga gtattatgaa gatgcagcta tcatgttcaa taacttgatt
    13201 gtcaaaaact tgacagcagc tgttcttgcc ggtatcggta ttgcgttgct gaatctgaat
    13261 gtattagctt ctgctttgac atggcaattt gttgtcttat gtctgacaag tgttatcgcg
    13321 atttcagttg tttcaggatt tgtcggtcgt ttgtttggtt tatatccaat tgaatcaact
    13381 attactgctg gcttatgcaa caactcgatg ggcggaacag gaaatgtagc cgtcttatct
    13441 gcagctaatc ggatggaact tattgctttt gctcaaatgg gcaatcgact tggtggtgcc
    13501 atcgttttga tcatctctgg tttcttgatg caattacttt cttagtcaaa aaaacaaaaa
    13561 tacctgatca tcaagtatcg gcttggtgat cgggtatttt tttgataaag atttacagga
    13621 ttttcaaaaa tcttttttgc attttatcgg aatgataacc gatgtgttca taaaattggt
    13681 gtgcttctac tctgtattct gcagaattaa gccttattgt cgttattcct cgttttttcg
    13741 cttcttcttc tacgccagtc atcaaagcct ttccgatgcc tcttttttca gcatatttag
    13801 acacagcaag tgccataata ttgaacattg gatcgctaaa aatcgattca taggactccg
    13861 cttgtacata gccagctgct tttttagaag taggatcttc ataaaccaag aaaaaatttt
    13921 tctctggctc cttcagcagt ttcttcattt gtctcaccgt aacggttaat ggtatatcat
    13981 accctaattg atccgcattg atttcacgta gtgcttctgc atcttctaaa cgcatttccc
    14041 gtaacatcca aatcaccagc caatttctga ttttttaaaa tgaatttttt tatcatagta
    14101 tactcttttt ttcatttaga acaatatgtt tcagtgattt tgacttgcgt tagtctagat
    14161 tctcctaatg tttgtaagat gacaaaggca ttgtaacaat tgaccaaaag aaccacgagg
    14221 acaattactt gaaaaataaa ggtcactttt tttggagaca aaaccctgct aaatcttgct
    14281 ccccacaaac ctccaataac agcagcgggg ataacataaa acaacataga taaatcaaag
    14341 cgcataaagc tacttgttaa agcaatcgtt accaattttg ccagttgaga aaagaaaatc
    14401 gtagatagag aatatagtgt tgcttctttt attggcaatg caaacatcag catcaacaaa
    14461 gaaacattga ctggtccacc accaatacct aaaaaactag ctaaaaagcc cagaaccccc
    14521 cccacatata aggtaccaag tccatgacgt caaatgaaat tttggctgat gatgcttggt
    14581 gtagaagaac gcaaaaacca aggtcaatac cgtcaaaaaa atttggatca gctgcacatg
    14641 cttctcgttt tcaaataact gtaaaaaaac ttcgaataca atgttgccga ctactcctcc
    14701 aacaacagct ccactagata cccaaccgac aatctgccaa ttcagtgatt tcccgcttga
    14761 tacttgtcgc acagtcgaaa caatggacat cgtgaatacc gcaactgttg agtaaaaaga
    14821 aatccctgca acagaatggg cgccgataag atcaagaatc ggtttgatca acacgcctcc
    14881 acccatcccg gaaatagcgc caactgtgtt cgccagaacg atcaccacaa aataaatcag
    14941 accaatcatc ttgtatttcc tttctcttgt ttatatcttt tagcgattgt tactcttttc
    15001 ttttattata cagtaaaaga aacaaacgag gcttcgaaag cttttctact ttttaaggaa
    15061 atttagtgct aaacgaaaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca
    15121 gccaaacaaa aatagctcct cataattttc gtttggttga acttattacc gcagatgcaa
    15181 gcttttgaac accgtttaag aaagaggtcg tgagcctgcc gttcagctcc gagtcacaag
    15241 gaacaatttc aaaaaacagc ttctcatgtt tgttgaatat tgtgacttgt gaccgaggag
    15301 ttggcaggag aacaccgttt aaggaagaga ctgtgaagtc aacgttttga cctgattatt
    15361 agagaaacaa atggcaaaat agttcttcat atttcgccgt ttttcgagcg aatgccgagg
    15421 ggcagctgat tgaacatcgt ttatt
//
LOCUS       DAZULY010000048        15352 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000048 DAZULY010000000
VERSION     DAZULY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.048"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..1061)
                     /locus_tag="ACG81A_000986"
     CDS             complement(93..1061)
                     /locus_tag="ACG81A_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFS5014371.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            1293..2126
                     /locus_tag="ACG81A_000987"
     CDS             1293..2126
                     /locus_tag="ACG81A_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFS5014372.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            2160..2999
                     /locus_tag="ACG81A_000988"
     CDS             2160..2999
                     /locus_tag="ACG81A_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5014373.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            3025..3585
                     /locus_tag="ACG81A_000989"
     CDS             3025..3585
                     /locus_tag="ACG81A_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5014374.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            3784..5505
                     /locus_tag="ACG81A_000990"
     CDS             3784..5505
                     /locus_tag="ACG81A_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFS5014375.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            5670..6812
                     /locus_tag="ACG81A_000991"
     CDS             5670..6812
                     /locus_tag="ACG81A_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS5014376.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            6828..8039
                     /gene="thiI"
                     /locus_tag="ACG81A_000992"
     CDS             6828..8039
                     /gene="thiI"
                     /locus_tag="ACG81A_000992"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFS5014377.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            8612..9259
                     /locus_tag="ACG81A_000993"
     CDS             8612..9259
                     /locus_tag="ACG81A_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFS5014378.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    9388..9604
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9652..12297
                     /locus_tag="ACG81A_000994"
     CDS             9652..12297
                     /locus_tag="ACG81A_000994"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFS5014379.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            12607..13920
                     /locus_tag="ACG81A_000995"
     CDS             12607..13920
                     /locus_tag="ACG81A_000995"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFS5014380.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            13910..14563
                     /locus_tag="ACG81A_000996"
     CDS             13910..14563
                     /locus_tag="ACG81A_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5014381.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            14618..15169
                     /gene="radC"
                     /locus_tag="ACG81A_000997"
                     /pseudo
     CDS             14618..15169
                     /gene="radC"
                     /locus_tag="ACG81A_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
BASE COUNT         5179 a         2624 c         3257 g         4292 t
ORIGIN      
        1 tctcaaattt ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc
       61 tctttttaag tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct
      121 tcacccgaca aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta
      181 atactgttac agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca
      241 taaacaggct ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg
      301 acaagaagct tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct
      361 tcgattacca aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc
      421 ccagcatatg ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt
      481 gtttctaaaa gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc
      541 aatgtaggtg ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca
      601 gaatggatct catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt
      661 gcgacacgaa tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa
      721 cgcattgccc cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa
      781 gcagtttctt ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca
      841 taagcggaca agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag
      901 ggaagattga agatatcttc aactaataat tctacatgct tggggattgt gatttctgca
      961 tctaataatg ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg
     1021 gcgatcgttc cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga
     1081 ataattttgc atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt
     1141 gaagaataac gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga
     1201 ctcaaccttg acctcctaat acaatggagt atctggttga gtttctttta caagaaagca
     1261 gccaaggaaa tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta
     1321 aagaacatga caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc
     1381 tacccttagt ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga
     1441 attggataag tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca
     1501 gcggtcaagg agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt
     1561 tagatgttat gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc
     1621 cctcatcatt caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt
     1681 ttgagggtta tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc
     1741 ttgaacgtac aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc
     1801 cacagttgct gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc
     1861 tttttcgatt ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta
     1921 aaaaaagaac gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca
     1981 aagtagttga ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg
     2041 atgcggctga aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca
     2101 atccaaccca aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta
     2161 tgatcaaagc tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt
     2221 tggaatcaac aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag
     2281 caacggggcg cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt
     2341 atgtcgtgta taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt
     2401 ttgaacaaaa agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg
     2461 tttttggtgc gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt
     2521 ttatcaagcg actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa
     2581 tcctccaggt ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa
     2641 aagaaccaat ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac
     2701 atcgctttcc cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa
     2761 aaggtggttc aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg
     2821 aagaaattat ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa
     2881 ttggagtggc catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc
     2941 aatcaaatga agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt
     3001 caatgaaaga aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca
     3061 ttccacaaaa cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa
     3121 gctcatacac gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca
     3181 cctaataaag aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca
     3241 gaagaaaaaa tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat
     3301 gcactgacag atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct
     3361 gatcgaatat tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa
     3421 cctcattatg accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat
     3481 gctccagaac ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag
     3541 aagaaatccg ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat
     3601 ttttgttatc tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg
     3661 aaaatagtat gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat
     3721 ttatgccaag cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca
     3781 taaatgggaa ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc
     3841 gtctttttgg ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa
     3901 aaacgcaagg aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa
     3961 atgcatctgg ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac
     4021 atatctactt cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat
     4081 gaaagttttc ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac
     4141 caaatggaga aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa
     4201 gaacgcaact cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa
     4261 gtcagagaag acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa
     4321 gtgatcgaaa aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa
     4381 gcccgcgagg ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa
     4441 aaaattccac cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc
     4501 agtgatactt acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct
     4561 gaagatatcg caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca
     4621 gaagttgaaa cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat
     4681 gatatcttag aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt
     4741 gcagagtata tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac
     4801 acatctcaaa gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc
     4861 gaagtggaag aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa
     4921 attccttatt cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc
     4981 gaaaagcaac aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa
     5041 gcgcaagaaa agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa
     5101 aaacaacgtt tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac
     5161 cgtattgaag aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat
     5221 cgtttagttg ctctatgtga agaagatcta gaattattag ataagaagac acatgattta
     5281 gtagatgcag cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca
     5341 cacgaagaag tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac
     5401 tatcaagaag cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc
     5461 aaacgcatcg aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt
     5521 aaaccagcaa gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa
     5581 attgaaacaa gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag
     5641 aaaagaattt caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc
     5701 caatctttcc gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc
     5761 catcaagctt gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc
     5821 agatcgctga tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag
     5881 aaggagataa ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata
     5941 tgatcatttc aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat
     6001 taggaatcga agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat
     6061 tgaaagaatt aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag
     6121 taggatcgat tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc
     6181 atttccatgt agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag
     6241 aaagagttga ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt
     6301 tcatttattg gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa
     6361 gaaatcagcg aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac
     6421 gtttatcatt agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc
     6481 taatcaatgc gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg
     6541 caccgcatat tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct
     6601 tagaagaaaa acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg
     6661 caagcagtac attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa
     6721 tcagcttaga tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc
     6781 agctttataa gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg
     6841 aaattatggt ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca
     6901 tgcaactagc acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg
     6961 ctgatcggga ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa
     7021 aactagcaaa aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg
     7081 tcgaagtttt gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa
     7141 catttaaaat tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga
     7201 atcaaacatt aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc
     7261 aaccagatat tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa
     7321 cgatcaaggg agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt
     7381 ctggggggat tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg
     7441 aagctgttca ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag
     7501 atctaacagc caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta
     7561 ccgagattca agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc
     7621 gtcgaatgat gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt
     7681 tgaatggtga atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca
     7741 atgaagtaac gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca
     7801 ttgaagtagc agaacagatc gatacattta atctttctat ccagcctttt gaagattgct
     7861 gtacgatttt tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat
     7921 atgaagagcg tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg
     7981 aagagataca agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag
     8041 aggttatgaa aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc
     8101 atgtatttta ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa
     8161 gaagttatgg aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat
     8221 caagctacag ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg
     8281 acagaagtgg gcagcttcaa gaaataagaa ggaatttacg aaaattgctt ttcaaatttt
     8341 tgtgaaattt cggcttattt ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt
     8401 gcgaaaaagc tgccctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat
     8461 tttacgtttg gttgagctta ttaccgcaga tgcaagcttt tgaacaccgt tttatttgtc
     8521 tcacaccctt ttccttccta cttgtaacct tgctcccgtg ttagaaacat gttacactaa
     8581 atatgtgaaa ttattaacaa ggagcgtcgt tatgcgagaa aaaacagata cactgccaaa
     8641 agcaactgcg aaaagattac ctttatattt acgctacttg aagatgctgg atgactcagg
     8701 tataagtaga atcaaatcaa atgagttcag cgaaatcacc caaattcctt ctgcaacgat
     8761 ccgcagagac ttctcgcaat taggcgagct aggcagaagc ggatatggct atgatgtccc
     8821 ttttcttatc gatgtattca ataatatcct taatactaaa gaagaaaaac gaatcgcatt
     8881 agtaggatat ggaaatcttg gcaaagcatt gaaacacaac aattttagaa gaaatgaaaa
     8941 tctaaatatc gtgtgcgtct ttgataatga tccagcactg atcaatcgag taattgatgg
     9001 agaaatgatc tatccgattg atcgtttcgc ggagatagcc aaagcaaaaa atgtgacagt
     9061 agcaatctct acggtaccaa gtaaatattc acaatctgct attgatgaaa ttgtcaaagg
     9121 aaatgtaacc gctatactta attttgcgcc tgacagagta actgtcccag cttatgtcaa
     9181 tgttcaatac attgatttga caaccgagtt gcagacgctt atttactttg atgagaatta
     9241 ctcagaagtt ttttcttagg aaaatttgag taggcttatt gatcttgcga gaactcgtcg
     9301 taattctttc aaaaattttt tagtactaaa tgaagtggtt ttttcacgac cacttgttga
     9361 ctaaaaaaaa aaaccattgt agaatgaagc ataacgctga acaagaggag taactggcac
     9421 attcattctt agagagagaa ctatctgctg aaaggttctc aatgacaacc agtgaaggta
     9481 gcttgggagt ttctgtcttg aatggagtag agatagacga gtcgtgatcg ttaactcact
     9541 tgagaggcaa taatgattga ttattgcaat ttaggtggta ccgcgtaaat catacgtcct
     9601 agaatacgaa agtattttag gacgtttttt agtttaaagg aggaaaacag catgacggaa
     9661 gaaaataacc tatcaacaaa atataatccg caagaagttg aagctggacg ttatcagcat
     9721 tggttagaac aagatttgtt taaaccgagc ggagataaaa aagcaaaacc ttattcaatc
     9781 gtaatcccgc ccccaaatgt tactggaaaa cttcatttag ggcatgcttg ggatacgacc
     9841 ttgcaagata tgctgattcg tcaaaagaga atgcaaggat atgatacttt atggctgcct
     9901 ggtatggacc atgcaggaat cgcgacacaa gcaaaagtag aagcgaagct tagagagcaa
     9961 gggatttctc gttatgatct aggtcgtgaa aagttcatcg atcaagtgtg ggaatggaaa
    10021 gaagagtatg cgagccacat ccgcgaacag tgggctaaaa tgggcttatc tttagattac
    10081 agtagggaac gttttacatt agatgatgga ttatctgaag cagtacgtaa agtttttgtt
    10141 accttatacg aaaaaggttt gatttatcgt ggtgaatata tcatcaactg ggatcctcaa
    10201 gcacgaacag ctctatctga tatcgaagtt attcataaag atatcgaagg cgccttttat
    10261 catatgagct atgaactagc agatggatca ggcgtagtag aaattgcgac aacccgtcca
    10321 gaaacgatgt tgggagatac ggcgattgct gttcatccag aagacgagcg ttatcaagcg
    10381 ctcatcggca aaaaagtgat tctaccttta gtcaataaac aaatcccaat cattgcagat
    10441 gaatatgtag atatggaatt cgggacaggt gtagtaaaaa tcactccagc acatgatcca
    10501 aacgactttg aggtaggtaa tcgtcatgat cttccacgcg taaatgttat ggatgatgat
    10561 ggtacgatga atgaactagc aggaaaatat gctggaatgg aacgttttgc tgctcgtaaa
    10621 gcaatcgtgc aagatctgaa agaaatcggt cgattgatta aaatcgatac gatgatccac
    10681 agtgtgggtc attccgagcg gacaggtgtc gttgttgaac ctcggctgtc tactcaatgg
    10741 tttgtaaaaa tggcgccgtt agcgaaaaaa gccattgata accaagaaac agatcaagca
    10801 gtggaattct tccctccaag attcaatcaa acgtttttac gttggatgga aaatgtccat
    10861 gattgggtaa tctcacgaca attatggtgg ggacatcaaa ttcctgcatg gtatcacaaa
    10921 gaaacaggtg aaatgtatgt agggatggaa gcaccagcgg atagtgaaaa ctgggtacaa
    10981 gatcctgatg ttttagacac ttggtttagt tcagctttgt ggcctttctc aacaatgggc
    11041 tggccagata cggaaagcga agattataag cgatacttcc ctactagcac tttagtaacc
    11101 ggttatgaca tcattttctt ctgggtaagc agaatgattt tccaaagctt ggaatttact
    11161 ggcgaacgtc catttgagaa tgtcttgatt catggattga ttcgtgacga acaagggcgg
    11221 aagatgagta aatcattagg taatggaatc gatccgatgg aagtaatcga aaagtatgga
    11281 gcagatgctc tccgctggtt cttatcgaat ggttctgcac caggacaaga tgtccgcttc
    11341 agctatgaaa aaatggatgc ttcttggaac tttatcaaca aaatctggaa tgcttcacgt
    11401 ttcgttatca tgaatatcga aggaatgacc gtagaagaga tcgatttatc tggagaaaaa
    11461 tcagtggctg accggtggat ccttactcgt ttgaatgaaa cgatcgaacg agtaactgag
    11521 ctatttgatc gttttgaatt tggtgaagca ggtcgacaac tgtataactt tatctgggat
    11581 gatttttgtg actggtacat cgaaatgagt aaagaagtac tttatggaga tgatgctgtt
    11641 tcaaaacaaa ccacccgtag catcttagtc catacacttg atcagatcct acgtttattg
    11701 catccaatca tgccattcgt tacagaagaa atatgggaac atattcctca tcagggaaat
    11761 tctctagtag tggcagaata tcctgttgta cgtccagagt ttgatgatga aacagcttct
    11821 aaagggatgg aagtattgaa agaattgatt cgagccgttc gaaatattcg ttcagaagtg
    11881 aatacaccat tgtctaaacc aattacgttg ctaattaaaa caaacgatgc taaaatcgat
    11941 caatttttag ttgaaaatac aagctatatc gaacgcttct gtaatcccga agagttgacg
    12001 atatcaagtg agatcactgc gccagatcta gcgatgtcag ctgttctgac aggtgcagaa
    12061 atcttcttgc cgttagcagg attgatcaat attgaagaag aaatcaaacg attagaaaaa
    12121 gaactggaaa agtggacaag cgaagtaaaa cgtgtccaag gaaaactttc taatgaacgt
    12181 tttgtttcta acgcgccgga agaagtagta gaggcagagc gtgcaaaaga aaaagattat
    12241 ttagagaagc aaacggctgt caaagaacga atcgaaagtt tgcgtactat tcaataagaa
    12301 tagaaaaaga gagtgtgaaa tcagctagct attactaaaa gctagctgat tgaacatcgt
    12361 tacaggaaag agtttgagcc tcactcttag cttcgattca taaggagatg tgacataaat
    12421 tttgtcgcat ctctttttta gtctactctt attatgtagg agaaggaggg aggtggatca
    12481 tgtatcaaga gtcaataaag tagttgataa aatcgttcct ctctctgttt cttttctctt
    12541 tgtcatactt ttatttttct tatgtgttct atttgttata ataaaagaga aatgggggaa
    12601 ttgttgatga cgattgaaga agcaatagaa tggatccata gtcgattacc atttggttca
    12661 cgtcctggcc ttgaccgagt cgaagcttta ttagaagtgg tagggcatcc agagagaaaa
    12721 gtaaaaacga tccatatcgc tggaacaaac ggaaaaggtt ctactgtagc ttatttgaga
    12781 agcctattag aagaggctgg gctgacagta ggtacattta cttcacctta tatcgaaacg
    12841 ttcaatgaac ggatcgcaat caatggcaaa agcattccag acaatgattt gatccagctt
    12901 gtaaaaaaat atcagccact gatcaaacaa cttgatcaat ttgaagcagt agctggtatt
    12961 actgaattcg agacattaac agcaatggca tttgattatt ttttgatgaa gaaagtggac
    13021 gtagcgatta tcgaagttgg cttaggcggc ttattggatt ctaccaatgt agctgtacca
    13081 atgctgacgg gaataacaac gattggactt gatcataccg aaatacttgg agatacaatt
    13141 gaagaaatcg ctgcacaaaa agctggaatc atcaaacaag gcgttccagt agtaacagga
    13201 aatattgccc cagaagcttt tactgtcatc caacaaacag ctaaagcaaa aaatgcaccg
    13261 ctttatgtat acggggaaaa ttataaaata aactatctgc atcctgatga gatgtgggga
    13321 gaagtatttg aattttttaa tgaaatagga aagatccctc aattaaccac tccgctttta
    13381 gggcaacatc aagctgaaaa tgcaggagta gccatcgaat tgtattttgt ttactgtcaa
    13441 ttggaaaagc tgcctttccg agaaaaagat gttttgaagg gattgaagaa atctcgatgg
    13501 ccaggaagaa tggagcgttt gagtaacgag ccattagttg ttttagatgg tgcccataat
    13561 acgcatgcaa tgaaccggct tgttgagaat ttgaaaaaag aattcaaagg atatcaaatc
    13621 aacattttgt tttccgcctt acgaactaaa aatgttagag aaatgctaga acgattgcag
    13681 caagtaccaa atgtccatat ttatttgacg acctttgaac atccaaaagc catcgaatta
    13741 agtgaagtat cagatttagc aagtgagaga gtatcaatcg tttcattgtg gcaattcgga
    13801 ttagcagata tattggaaaa gatgacaaat gaagacgtat tagttgtgac cggttcactt
    13861 tatttcattt cagaagttcg ccatttatta atacaattag gaggagatca tgaagtataa
    13921 tgcagtgatt tttgacatgg atggtttgct gttcgacaca gagttagtgt attacgaagc
    13981 cacacaagta gtagcagaca agatgggttt tccttatgat aaagacctgt atttaaaatt
    14041 cttaggtgtt tcagatgaag aagtatgggc gaattatcat atgattttct cggaatttgg
    14101 caaaacaagt gtacaaagat tcatagatga ttcataccag gaaacgcttg aacgtttttc
    14161 gcaaggagcg gtacaaatga agcccggtgt gttggaactc ctctcttttt tagaagaaca
    14221 tcagattccc aaagttgtcg cttccagtaa ccaacgagca gtcattgaac ttttattgaa
    14281 gaagaacaat ctgtttgaac gatttgagac gattgtttct gcagaaaatg ttcaacgagc
    14341 aaaaccagac ccagaaattt tcttgttagc taatcagaag ctaggtacag cgaagaaaga
    14401 aacattgatt ttagaagatt cacaaaatgg tattttagca gcttctgcgg ctgagatccc
    14461 ggtagtcatg gtaccagatc tgcttccacc atcccaagaa ttgatcaaaa aaacagctgg
    14521 ggttgtttct tctttacatg aaatccctgc attgttaaaa taaaaatcca tctcttttcg
    14581 tatcttattt tagataagat agaaaggaga tgtttttttg ggaaaacgat tgatcaaaga
    14641 agtacctact agctctttac caagggaacg tatggaaatt tatggcgcgg aagctttatc
    14701 ggatcaggag cttttggcaa tcttactgcg taccggtcaa cacccttata gtgtgatgtc
    14761 gattgctgga aatctgttaa aaacgtttgg aggactggcc tctttacgac aggctacttt
    14821 gcatgagtta gaagagattc gagggatagg acgcgtgaaa gcaatcgaaa tcaaagcctt
    14881 gatcgaatta ggtcgacgaa ttcagacgga tcatgaaaga gcggctccac cggttcgtac
    14941 cagctacgaa ttggcccagc aattgattct ggaaatgaaa gatcacaaac aagaacattt
    15001 ggtttgtatt tatttggaca cgaagaatca agtgatctta aaaaaaacag tgttcatcgg
    15061 ttcgctgaat cagtcagtcg ctcatcctag agaaattttc cattatgctg tacgttattg
    15121 tgcagcaagg atcgttttag cgcataatca tccttctggt aatgttttaa ccatagtata
    15181 ataaataata gtagttaaag tagtttaaga ggagatatta aagggatatt ttactttaaa
    15241 taaaaattaa caaggtttat aatttgtagt agttaaagta tttaaagagg tgggaacatg
    15301 tgaaggttca aaggatagaa gtggagaata agccgtatcc attgtattta tt
//
LOCUS       DAZULY010000049        15167 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000049 DAZULY010000000
VERSION     DAZULY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.049"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACG81A_000998"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="ACG81A_000998"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFS5014382.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACG81A_000999"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="ACG81A_000999"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFS5014383.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACG81A_001000"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="ACG81A_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFS5014384.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACG81A_001001"
     CDS             complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="ACG81A_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFS5014385.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACG81A_001002"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="ACG81A_001002"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFS5014386.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACG81A_001003"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="ACG81A_001003"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFS5014387.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="ACG81A_001004"
     CDS             complement(8054..8524)
                     /locus_tag="ACG81A_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFS5014388.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="ACG81A_001005"
     CDS             complement(8570..8995)
                     /locus_tag="ACG81A_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFS5014389.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="ACG81A_001006"
     CDS             complement(9062..9331)
                     /locus_tag="ACG81A_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFS5014390.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="ACG81A_001007"
     CDS             complement(9342..10937)
                     /locus_tag="ACG81A_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS5014391.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACG81A_001008"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="ACG81A_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFS5014392.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACG81A_001009"
     CDS             complement(14490..15050)
                     /gene="pth"
                     /locus_tag="ACG81A_001009"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFS5014393.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
BASE COUNT         3920 a         3436 c         2688 g         5123 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agctttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt cccctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggaa
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttacac ttgtcaatgc atagtataac acaaaac
//
LOCUS       DAZULY010000050        14815 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000050 DAZULY010000000
VERSION     DAZULY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.050"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACG81A_001010"
     CDS             complement(296..1024)
                     /locus_tag="ACG81A_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5014394.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACG81A_001011"
     CDS             complement(1002..2576)
                     /locus_tag="ACG81A_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS5014395.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACG81A_001012"
     CDS             2805..3743
                     /locus_tag="ACG81A_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5014396.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACG81A_001013"
     CDS             3755..4666
                     /locus_tag="ACG81A_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5014397.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACG81A_001014"
     CDS             4690..6162
                     /locus_tag="ACG81A_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5014398.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACG81A_001015"
     CDS             6213..6824
                     /locus_tag="ACG81A_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5014399.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACG81A_001016"
     CDS             6854..8767
                     /locus_tag="ACG81A_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFS5014400.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACG81A_001017"
     CDS             8895..11876
                     /locus_tag="ACG81A_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFS5014401.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACG81A_001018"
     CDS             11873..13534
                     /locus_tag="ACG81A_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFS5014402.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACG81A_001019"
     CDS             13574..14506
                     /locus_tag="ACG81A_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFS5014403.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DAZULY010000051        14607 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000051 DAZULY010000000
VERSION     DAZULY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.051"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            67..963
                     /locus_tag="ACG81A_001020"
     CDS             67..963
                     /locus_tag="ACG81A_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFS5014404.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1113..2054)
                     /locus_tag="ACG81A_001021"
     CDS             complement(1113..2054)
                     /locus_tag="ACG81A_001021"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS5014405.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2180..3301
                     /locus_tag="ACG81A_001022"
     CDS             2180..3301
                     /locus_tag="ACG81A_001022"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS5014406.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3559..5061
                     /gene="eat(A)"
                     /locus_tag="ACG81A_001023"
     CDS             3559..5061
                     /gene="eat(A)"
                     /locus_tag="ACG81A_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS5014407.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5128..5655
                     /locus_tag="ACG81A_001024"
     CDS             5128..5655
                     /locus_tag="ACG81A_001024"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5014408.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5866..6927
                     /locus_tag="ACG81A_001025"
     CDS             5866..6927
                     /locus_tag="ACG81A_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFS5014409.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6930..7826
                     /gene="murQ"
                     /locus_tag="ACG81A_001026"
     CDS             6930..7826
                     /gene="murQ"
                     /locus_tag="ACG81A_001026"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS5014410.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7840..9822
                     /locus_tag="ACG81A_001027"
     CDS             7840..9822
                     /locus_tag="ACG81A_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFS5014411.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9887..10333)
                     /gene="nrdI"
                     /locus_tag="ACG81A_001028"
     CDS             complement(9887..10333)
                     /gene="nrdI"
                     /locus_tag="ACG81A_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS5014412.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10544..11968
                     /locus_tag="ACG81A_001029"
     CDS             10544..11968
                     /locus_tag="ACG81A_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFS5014413.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12149..12922)
                     /locus_tag="ACG81A_001030"
     CDS             complement(12149..12922)
                     /locus_tag="ACG81A_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5014414.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12919..13824)
                     /locus_tag="ACG81A_001031"
     CDS             complement(12919..13824)
                     /locus_tag="ACG81A_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5014415.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(13978..14298)
                     /locus_tag="ACG81A_001032"
     CDS             complement(13978..14298)
                     /locus_tag="ACG81A_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFS5014416.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         4663 a         2607 c         3096 g         4241 t
ORIGIN      
        1 actggctttt tactatttaa acaggctatc ataggaaaaa gtgacagaga aagtgaggcg
       61 gaaaagatga acaaaaatga cgtactgcta gttgcaaaaa ataaatcact ttattttttg
      121 ccttccatcc gctctttaga tgtttggtcc ggaaaagatt tactacagtc ggaattcatt
      181 aaatcagcta gacaacagtt ggcaaattat cagctgatcg tttttttaga ttatggattt
      241 gatatggaga tggctcgacg cgtgcgccca tttaccaatg caaaaattgt gttatttttt
      301 tggaatcact tcaaagcaga acacgaggcg atgctgaagc aggcacaaga tgaaccggca
      361 atcgatgaga tttatcattt tgattttttg gaagcaaaag aacttgggct gaaacataat
      421 agttcatttt attcgaaaca tatgaatttt tcttctagcg aaccaaagac ggatctattt
      481 tttggtgcaa cgaataatgg gagaaaagaa cgagcagaaa gctataagca ggaatttata
      541 aaaagagatg ttacgaccaa ctatttcatt cttccacatc gagggaatga acaacctgga
      601 tatcttactt atgaagagta cctgaaaaaa acaggggaat cgcgcgggat tctggaactg
      661 ttgcgggagg gtcagcaagg agtgactttg cggacatttg aatccatgta ttttcaaaag
      721 aaactagtga cggataatca agcgattgct cattatcatt tttatcgtcc tgaaaatatt
      781 tttttgcttc gagaacgtga tttagatgaa ttaccggaat ttctgcatac accatttaag
      841 ccaatcctat cagagctatt ggaattttat gatgcagaaa attgggcaaa acgatttatc
      901 actaacgatt ccactattta tgagcaatac gaatatgacc tctcctctgt tgaaaacaaa
      961 taaaaaacct acattttgaa gagattatgt actgaaacaa acaagcacaa ttcttatggc
     1021 atcatgagag ttgtgttttc tttttgatct tttattgggc ttaaaaaaag agaaccagct
     1081 gctgattcag ctgattctca tcagagagcc ttttattctg cgccaaggat tccttctttt
     1141 gcatggacta attgggtcaa aaaggcacaa tatggtgtag caatatcata tttttgtcct
     1201 tttcgccaaa tggcaccatt gatgtagtcg atttcagtca aacgatggtt tttgatcagg
     1261 tcttggtaca tagatgggta atgcaacccg atgttgtcag gatcataagt agaagcaata
     1321 tgttggtaca cttcttcttg atctagtatg attccttctt ttgctgcgat agaagcaaat
     1381 tcagatacga ttgttcgaac catgctctct gccgcttttt gcgcgcctag ttcagcaata
     1441 ttacaatcca aaatcgtgca taaaccattc aatgttccat ttacacaagc ttttcgccaa
     1501 atggaataac gaacgttatg gctatagatc ggatttaaat ttgcttcttg aaagacttct
     1561 acaacttttt ttgtaaatgc ttcgccttct ggttccaagt tctctagttc gatttctcca
     1621 tcacctaaca atgtgacttt tccgggacca gctagaccag ctgtccacat cgtgatccca
     1681 aataagatat ttttctttgg cacatatttt tccagtacat cttcatgacc taatccattt
     1741 agtaaacaca aaacataagt attgtcagta ataatggatt ggatcgaaca aaacatatca
     1801 tctaattgat tggcttttgt taatgctacg atcaagtcca cgtgttcgtt gctttcgatg
     1861 atttcttctg gtgaatagat tggcagtttt gcgaccactt ctttaccgtt aaagtcagca
     1921 atcaacccat ttgtacgaat ggcttcaatg tgagcaggcc aacgatcgat caagaggacc
     1981 tcatttccgc tttgatgtag catcaatccg attcggctgc ccattgctcc ggccccagct
     2041 attgcgattt tcatgttttt tctctccttt attcaatgag ttccctttta gtatatagaa
     2101 aaatggaaga aaatagcatt gttttgtttg aaaaaaatga aaaaaagtgt cttacttaag
     2161 aagaaagtga gggatacaga tggatatttt ggcggcaatt gattcattta aaggaagtgc
     2221 ttcttcattt gaactaaatc aggcagttct aaatggctgg gaagatcgta aaggaagaaa
     2281 aatcaatgtg ccgattgcag atggcggaga aaatactgca gaagcaatct attacgcgct
     2341 caggggaaca tggcgtacca ttgatgggat ggatctctta ttccgtcagc gaaaaatcag
     2401 ctatttattg acaacttggc aaggaaaaaa caccgcagtc atcgaatctg cacaagcatt
     2461 ggggttagat ctacttgata aaccaacgga tcaaactatc cgacttgctt ccagttatgc
     2521 cttaggagaa cttattaaag atgcactgct tcaagaagta gaacaaatta ttgtcacttt
     2581 aggaggaagc gggtgtagcg atggtggtct aggattactc caatccttgg gtgcttcttt
     2641 agaagggtac acagaaggaa atcctttatt atctactaaa aaacttcatt ttgatcgaag
     2701 aaacgttctt gtttctaaag taaaattgat tatcgctgca gatgttacca atccatatac
     2761 aggtcaacaa ggagctgctt ggcaatttgg aagacaaaaa ggaggaatag atacgacgct
     2821 agctttctta gataaacaag caggaaagat tgctcagcaa ataaaagatt tctgggaggt
     2881 ggatttagat gatattccag gaagtggagc agcaggcggt attggtggag cccttttttt
     2941 gttgggagcg aagatggttt ctggtttcga tttagtcagc caaatcactg gattggaaaa
     3001 aaagatcgaa caagcagatg tcatcattac aggagaaggg cggatcgatc agcaaacgat
     3061 ccacggaaaa gttccatacg gtgtagcagt gcttgcaaaa aaatatcgaa aaccaataat
     3121 cgctatttgc ggaagtagag accaagagct agaaggactt tctgattttc tcccaattgt
     3181 cttaagtatt catttgggtc caataggttt agaagaagca atggaacaca ggcaaacatt
     3241 agaaaaaaca aaaatcttag gacagacact tagcagattt ttcaccatcc tcccgaagta
     3301 gttgacaaaa tcattttctt tatgctatcg taatttcaaa tataggacaa agtgagggat
     3361 ccgtgcattg aaaaattaag tcgattgatg aaagctgcat tcagactgta ccttgtggag
     3421 acaaggaagc agtgtgcaga ttttcgggat tggcttggtt ttttcgatgt atgggtcttt
     3481 ttgtgtgtat ccaacccttg catcctcttt agccagttct gaaaatagga ctggtctttt
     3541 tttacgaggt gatggaaaat gtctaaaatc gaaataaaaa atctgacatt cggctacgac
     3601 agccaaggca cattattatt tgaacaagca aatctaaatt ttgacacaca atggaaacta
     3661 ggacttatcg gacgaaacgg tcgaggaaag acaactttac tgaatattct acaaaacaaa
     3721 ctaccttatc aagggcaagt aatccatcag caagaatttg cctatttccc gcaacagaca
     3781 aaagataaag aacgtttaac ctattacgtg ttaaatgata ttacggattt tgagatatgg
     3841 gaaatcgaaa gagagctcca attgatgcaa acagatcctg aaatcttatg gagagaattc
     3901 agcacactat cggggggaga gaagacaaaa gtcctactgg cacttttatt tgtggatgac
     3961 actcatttcc cgttaatcga tgaaccaacg aatcatttgg atatctctgg tagaaaacaa
     4021 gtagcggctt atttgaaaaa gaaaaaacaa ggcttcatcg tggtcagcca tgaccgggga
     4081 tttatcgatg aagtagtgga ccatgtttta gcaatcgaaa aaagtcaact ggaactttat
     4141 caagggaatt tctctatcta tgaagaacag aaaaaacttc gtgatgaatt tgaaatggct
     4201 caaaatgaaa aattgaaaaa agaagtcagt aggctaaaga aaacagcagc tgaaaaagcc
     4261 gaatggtctc gttcccgaga aggagataaa acaaagaaac aagtcggatt catcgatact
     4321 gaatctagac gagtgaataa aggagcagtg ggtgctgatg ctgcacggac gatgaaacga
     4381 tccaaagcaa tcgtgaatcg gatggagacc cagatcagcg agaaagaaaa actattaaaa
     4441 gatatcgaat atatcgattc tttgacgatg aatagccaag cgtctcacca taagcgactt
     4501 ttaagcgtag aagatcttca attagggtat gaaaatctgt tattcgagcc aattcatttt
     4561 acaatcgagc ctcatcagcg ggtggcgatt tcaggtccta acggtgcagg aaagtcatcc
     4621 attatccatt atcttctggg ggcattcaac ggcaaggtta taggagaaaa ataccagcca
     4681 aaacatctga gcattagtta tgcaagccaa aattatgaag acaatcgagg aacgttggcg
     4741 gaatttgcag agaaaaacca agtagactac caagcatttt tgaacaacct ccgaaagctt
     4801 gggatggaaa gagatgtttt tcataacaag atcgagcaga tgagtatggg ccaacggaaa
     4861 aaagtggaat tggctaaatc tttatcacag ccagctgaac tatatacatg ggatgaacca
     4921 ttgaattatt tggatgtctt caatcaagaa caattagaac aactgatctt gaacgtgaaa
     4981 cctgccatgt tactagtgga acatgatcaa accttcctgg ataaagtatc tactgagatt
     5041 atttctcttg agagaatcta acccttgtaa tctcaagaac aacaaggtat ggttaagggg
     5101 aactttgcca acgaaggaga gaatcacatg ctttatgtaa cgagacacgg agaaacaact
     5161 tggaacgcac aaggattagt ttgcggacgg gcagatgtgc cgctgactga aaaaggacag
     5221 atgcaggcac agaaactagc agaaaaggta gtagatttgc ctgttccaat cacgaaaatc
     5281 atccattccc ctcttcaacg agcaagagat acagcacaag cagttgctga tcgtctcagc
     5341 ttgccgctga ccgttgatga acgattggta gaaatggatt ttggggatta tgatggactg
     5401 ccaagcaaag atgagaactt tcaaaaagca cgtttagcgt tcgctgtacg attcccaaat
     5461 ggggagtcag tcttagatgt gtatgcacgg atcgttcctt tattgaaaga atgcatcgaa
     5521 gacgaagaaa atgtctatct gttagtttgc cataatgcct tgataagagt gatcaatgct
     5581 tattttcatc cgatgcctaa tgaggggttc tttacattca tggtagataa tacagaattg
     5641 gtttcttatg agtaattatg tttttgtgtt atttaaataa aatatttcaa ggcactgctt
     5701 ttgcttagca gtgccttttt taggttatga caattctttt gataatataa actcaacctt
     5761 tagaaatgaa atttaattat tttaataaaa gtattgaaat tttgtttctt tagatatata
     5821 ctctagtcat agaaatgaag acggatacag aaaaagaggg ataatatgtt tggcttttca
     5881 atatttatga acgattcttt gtcagaagag aagaaacagt atatccgcca aatggcatcc
     5941 aatggctttg tcggtatttt cacttccatg catataccag aagatgatgt ttcggcatat
     6001 caaaagcgat tgatcgaact aggaagcgaa gcgaaaaaga ataaactaga attgatggtc
     6061 gatatttcag gagaggcatt ggaacgagcg ggtttttcat tagaagatct aacgcctttg
     6121 aaagcaattg gtgtaacagg tttaagaatg gattatcata tttccaatca gcagattgcc
     6181 gactggtcac atcaattgaa aatcagtttg aatgctagca cgatcacgcc taaagatatc
     6241 gatgaattga aagaagcaga agctgatttc tcacagattg aagcttggca taattattat
     6301 ccacgtcccg aaacagggct ggacaaagaa tggtatcaga aaaaaaatca atggctcaaa
     6361 attcaaggtc tccttgtcca aggttttgta ccaggagata cagagttgag aggcccacta
     6421 tatcaaggat tgccaacttt ggaagaacat cgagggatcc atcctcttgc aggcgctcta
     6481 gatctattag caagtaacac ggatattgtt tacatcggag atgcaggtct gtcagaaaac
     6541 gttcaggagc aatttgcttc tttccaaaaa gaacagacag tcttgctcca tacggaacct
     6601 gtagatgaag aattttacga atatatatta gggaagcata cgaatcggca agacgatgca
     6661 agagatgtca tacgaagcgt agatgcacgt tttcgagaga ttccgcctat cccagcaaga
     6721 aatacagcta cgcgtatgaa agggtctatt actttagata acgaaaagta tttgcgctat
     6781 atgggtgaga tccaattaac caaatatgat ttgccagcgg atgaaaaagt gaatgtagta
     6841 gctaaagtta tcaaagaaga gctaccattg atcaaccaaa tcaaagctgg tatgaactat
     6901 caatttataa gaaaagaagg aagataaaaa tgagcaaaat caatttagag aacctaacaa
     6961 cggaacgcag aaaccaagaa acttttggat tagatgagat gagtatttct caagccgttc
     7021 aactaatgaa caaagaagat cagaaagtgg ccgctgcggt tgaaaaagaa ctgacaagta
     7081 tcgaaccagt gattgctaac acgattgagt cctttaaaaa aggtggacgt ctgatttata
     7141 tgggagccgg aacaagtgga agactaggtg tgttagatgc tgctgaatgc gtaccgacat
     7201 tcggtgtaga accggaaatg gtcataggtc taattgctgg tggagaagaa gcaatgactg
     7261 tagctgtcga aggggccgaa gattcttatg agctagggca agaagatcta atcaagctga
     7321 atttgacaaa aaatgatata gttattggta tcgcagctag cggtcgtaca ccatatgtga
     7381 tcggtggatt agattacgca aaatcgatcg gtgctgtcac aggaacgatt tcttgcaata
     7441 aacaagccga aatcagtaaa cacgcggatt tcccaatcga agtcgatgca ggtccagaat
     7501 ttttgacagg ctctactcga ttgaagtcag gaacagcaca aaaacttatc ttgaatatga
     7561 tttctactat cagtatgatt ggtataggta aagtattcaa taacttgatg gtcgatgtca
     7621 aaccaacaaa tgaaaaacta atcgaacgct caaaacgaat catcatgcaa gcgacagaag
     7681 ctgattacga aacagctgaa aagtactttg aagaagcaga tcaaaatgtt aagctggcta
     7741 tcgtcatgat tttgacaaat agtgataaag aggaagctca agcaaaatta gctgaagcaa
     7801 acggatttgt aaaagggacc ttatgagggg gaaaagacca tggcaaaaaa cgaactgact
     7861 ttggaagaac tggcaaaaca aatttataaa ggggcaggcg gcatgggcaa tgtagaaagc
     7921 attgtccact gcatgactcg tgtgcgtatg aagatcatca atgatgatct tgtaaatgtc
     7981 tctgaattaa aagcgattcc aggagtgctt ggtgtagtag aagatgaaca gcttcaagtc
     8041 atcatcggac ctgggaaagt gaacaaagta gcacagcaaa tggttgattt ggcaggcgtg
     8101 aaattaggag aaaaactttc ttctgtaagt acagctacag caaattcatc agcatctgga
     8161 aaagaccgtg taaacgaacg ggcacaagaa atgaaagcgg cacaaaaatc gaaacaaaaa
     8221 ccatctaaaa tcaaatcggt attaaaagac atttctaata tctttgtgcc aatgattcct
     8281 gcatttgtcg gtactgggat cgtggcaggg attgccgcag ttttagcaaa cttagtcaca
     8341 gccggaacac ttgatgcagc aacatggcaa caatatatcg atgtgatgaa catcttgaaa
     8401 aacgggatgt ttagctacct agttatctat acaggtatca atgctgcaca agtttttggt
     8461 gcaacaccaa ctcttggtgg agttatcggg gccgttgtga tgctgacagg gatgaatccg
     8521 gaagcaccac tgaaaaactt atttactggt gaagctcttg cagctggaca aggtgggatc
     8581 ctaggtgtca tcttcgctgt ttggctattg tcaatcgttg agaaaaaact gcataaagta
     8641 gtacctgatg cggtagatat catcgttaca ccaactcttt cattgattgc catcggtctg
     8701 attgaaattt tcttcatcat gcctttagca ggtttcattt ctgatggcat ggtcggtggg
     8761 atcaactggg tacttggcgt tggtggagca tttgccggct tcgttctagg cacattattc
     8821 ttgccgatgg ttatgttcgg actgcatcaa attttgactc caatccatgt gcaaatgatt
     8881 gatgaaactg gtcgtacgct attgcttcca attcttgcga tggctggtgc aggacaagtg
     8941 ggtgcagcat tggcattatg gattcgctgc aaaaaagata aagaattaac agaaatgatc
     9001 aaaggagctt taccagtcgg gatcctagga atcggcgaac cattgattta cggagtaaca
     9061 ttacctctag gacgcccatt tattactgct tgtatcggtg gaggtatcgg cggggcagtg
     9121 atcggaatga tcggaaatgt cggggctatc gctattggac cttcaggagc cgctttgatc
     9181 ccattgatct ctgacggtaa atggttaggc tacatccttg gattattggc agcttatgct
     9241 ggtggcttcg ttgctacatt cttcttcggt attcctaaag accgtttaga aaaagaagaa
     9301 ttagcgactg aaacagccac agtaaatgaa gtgcctgaaa aagtagtacc tcaaacttca
     9361 gctgaaagtg tgactatcct ctcatcagtt gctgacggaa cagttgtttc tttagataaa
     9421 gcaagtgatc cagtttttgc acaaaagatg atgggtgaag gctactttgt agaaccagaa
     9481 aacggacata tctattctcc agttactggg gtagtcagct ctgtcttccc aacgaaacac
     9541 gctattggga tcacaacgcc aaatggttta gaaatcttgc ttcatatggg aatcaacaca
     9601 gttgacttag gcggtacgcc atttgatgtg aaagtaacag aagggcagca agtgacacct
     9661 gatacgcttg tagcagaagc tgacttagaa gcgatcaaag cagcaggcaa agaaacatcc
     9721 atgatcgtct tggtaaccaa tatggatcgt gtaaaaaact ttgtcttgga aaaaacaggt
     9781 aaagcaaaag caaaagcacc tgtgatggat gtcaaagctt aatatgatag aaacaataga
     9841 aacgaaagaa gaaactgaaa tctatttttc agcttcttct tttttattat ttattgtttt
     9901 ctgctaaaat ctgataaatt cgtgtcacat cagatggtgt tccccgtaat tcataatctg
     9961 ccaagaaagg aaagccaaat gtttgtgcat attgtttggc tgttagacaa tactgattat
    10021 tgaagttttt atttccgcta ccgactacac caagacaaag tttatgattg tcttcatgtt
    10081 ccagatattc acgcatcgtt tcggtcaaga tttctgtatc accgttatct aaaccatttc
    10141 cgccgtctaa ataagtagga acgaaagtaa aaaatggttc tgtttctttc gtaaaatcac
    10201 tattttcatg gatttctttt aaagtaactt caggattgac aggatcttct gcatgcattt
    10261 tttcagcata ttctgctaga tgtttggcaa aggcacgcgt atttcctgaa atcgatatat
    10321 ataaaatgtt catggatcat cctccaattc tttcctttta gtatagcatc ttttttaagt
    10381 tttttggtgg tgtaatttgg aaagggacta ggcgctttgc ttgtataatg agaaaaaagc
    10441 cggtagaatt atatttctat taaagttttt attaattaag taaaaataaa aaaggtttgt
    10501 tatacttttt gataaaagcg gttttatttt ggaggatgct tacatggata cagagcaaca
    10561 gaaaaaagga attggtttaa ttccacttgc tgccttagta attggttcag cgattggagg
    10621 tggcgtattt ggtattatga cggacatctc cggatcggct ggcggcccag ctattttcag
    10681 ttgggtactc gtaggaacag caatgttgat gctctccttg tcgatcaata atatcaataa
    10741 aaaacgtcca gatttaaaag gcgggatctt tagttacgca gaagaaggtt ttggccgttt
    10801 cgctggtttt atcagcggtt ggggttactg gctcacatgc tggttaggca acgtagcttt
    10861 tgcaacattg ttgatgagcg cattaggata tttcttccca gtatttgaag gtgggcaaaa
    10921 tctggcatcg atcgtgatga gtacagtctt tttatgggga tatgcatggc tagtgaatcg
    10981 gggagtcgaa aatgctagtt tcgtcaatgc tatcgtcaca ttctgtaaat tagtgccatt
    11041 atttttattt attgtagtag ctatcagcac gttttcgatc caacaattca ttgataactt
    11101 ttctagcgac attatattga atgcgaatgg acagccgatc ccgttttcca gtcaagtatt
    11161 gagttgcatc atggtgatgc tttgggtatt tgttggtatt gaaggagcct ctgtcgtagg
    11221 ttcacgagca aaaaaacaat cagatgttgg gaaagcaacg attctaggga tattcggctt
    11281 gattattatt tacgtcttgg tttccatgct tccatatggg acaatgacga tgaatcagct
    11341 gcaaacgatc aaggcgcagc ctgctatggg ctatgtgttt gaaatgatgg ttggtaaatg
    11401 gggagcagtc gtgatcaacg gcggtttgat catttcttta gttggtgttt ggctctcatg
    11461 gacgatctta ccgattgaaa caatgggaaa catggctgaa gacggacttt tgcctaaagc
    11521 atggggaaaa ctcaacaaaa atggggcacc aacttatgct atcgtattga cgacgttatg
    11581 tacgaatgtc tttttacttt cattattatt cactgacagt gcttataact ttgcttacac
    11641 acttggtaca gcagctattt tcttcacgtg gcttttctta ggattgtacc aaatgaaact
    11701 ttcttatcag cggaaagaat gggtccaatt tttagtaggg cttttagcaa gtgcctttca
    11761 agtatgggct atgctagtcg tagcctttaa tgaagtaatg cttgttcttg ttcttttctt
    11821 gccaggcatt tatttttacc tcaaagcacg taaggagcaa aacgagcctg tacaacatat
    11881 ccacttagat aagattttac ttataggtgt gacgatggtc ggagtgattg caatggtttt
    11941 atttgccact gggatgcttc acgtatagaa gtataaaaga agtataaaga gaggttgtga
    12001 gcctgtcgtt tattcaaaaa catactagga aaggagctgt gacacaaatg ttgtcacagc
    12061 tcctttttca gaataaacgg tgtttaagag acagccaatc ggcaataagg ctgaacgtct
    12121 ctttcacagc ctcttttttg atgagtcatc atacattttt tcgcttaaat acgagatatc
    12181 ctaatgctaa gaacaaaacg atgtaagcta aattaccgaa tagcatttga ttagtagaaa
    12241 gatgagtcaa ttgcttcatg atctcttcgt ttccaatttg gttttgcaga ttcaacatac
    12301 tgatcggatt ccatttgatc cactcccatt tttggatgag tgcaataagg ataccagata
    12361 tgattgacga agcaaaataa aagacgattc ctgctgagat ggatgcgccg gaactattga
    12421 taaagcaagc aatcatcaac actaaactta aaatcagcca taaaccgata taactaccca
    12481 aagtataagt gaacaagctt tggatcagtg tttgaccagt agaaaccttc tccgtgaagg
    12541 aaatcgaagg gaaaagaatc agtttcatca acacagtgac aataatagct aaaaggtata
    12601 aataaacaga ataaataact aatgtgatcc atttgcttac taatatttct cctcgagaat
    12661 actttcgata aaggagattt ttcaatgtac catattgtgc ttccatcgaa atgatcgtgc
    12721 ttgcagctgc aatcatgata aagacaatcc aagatgtggc actaaaaagc tgagcagtca
    12781 tttcaactgg atcaatgatc gtcgtgtatt ttttggctag aagtgctgtt cctatgagca
    12841 aaacaactaa tagaacagat aaaacagcag ttgacttttt cttgatcaat ttaaataatt
    12901 cttgttttac aagcaatgtc atttttcttc ttcccccttt actgaatcca gaatttccaa
    12961 tagagaagat tccaaatcat tcgtcagatg ctggacgtct gtcacatgga tattttgtgc
    13021 agacaatagc tggatcacgg ttcctaataa ttcttcgttt tcttttaaaa gcttgatttt
    13081 atcaggatct tccaaagtat atccagcttc tgtcaagatc gttcgagcag ctagatcatt
    13141 tgttgtagag ataacgatga aatcttgcgc tgtttggttc aattcagcca ttgtacattg
    13201 tttgatgatc tttccttgat tgatcaggat cagctcatcg accatttttt caagctcgct
    13261 gagtaagtga ctggagatca agaaagaggt gccctgagta gccaagtcgg taatgatttt
    13321 tcgcacatct cttgttgctt gtggatccaa accgttcatc ggctcatcta aaataacgat
    13381 agacggatgg ttcagtaagg caagagcaat tcccaatttt tgtttcattc ccaacgagta
    13441 ggatttcgct tttttgtgaa tgtacttttc catcttgagc tgattcacga tagtgtcgat
    13501 agcagataca tcgttggttt cagagaataa cttcagatga tcatagcctg ttaagaatgg
    13561 atagataccc gggtattcga tcaatgctcc tacttgttcc aaccctttat gactagaggg
    13621 agaaatctca ttttcaccaa tacagattgt tccatctgag taatggatca gtcctaaaat
    13681 agctttcatg atcgttgttt ttccagcgcc attaggacct actaatccaa cgattttgcc
    13741 aggggataag ctaaaactga catcgctgag ggcttgtttg tgaccaaagt tcttggataa
    13801 ttcttggacc gaaagacttt tcaaaaatat tcactccttt tctatagata gtacaagcat
    13861 acacctgatg agtggagaag tcgctaggtc attggactta tttcttattt tttcataaaa
    13921 aaattgaata ttaaataaaa aggcttattt tttctgatca gtacttattc caagtgtcta
    13981 gtggccgcta gtaatcttga tgccaatgat actgatgagt agcatcccca caaagaacca
    14041 agtggaaaga ttcagcttat catgaaacaa gaccacacca attatgaccg aaccaactgc
    14101 gccaatccct gtccagatag gataagagag actcatcggc aaacttttga ttgcctttga
    14161 caaaaagtaa aaactagcaa tcatgccaat cactgtgtaa aaagaataat tgattttaga
    14221 aaaaccatca ctccatttca tcatcgttga ccagaatact tccaaaattc ctgctatgat
    14281 taattccatc catgccatac ttacattcct ccttaactat aaaaaaatac acaaaaagag
    14341 gaaaatcatc ccttcaaaga cctaatggaa tgatcatcct ctttatccta cccggtggca
    14401 gattgtatat ctttttctgt agtatagcac acctatccag atatcggcaa tctcatactt
    14461 tttagtaaag tgtaccaagc actgacggat atatcgttag cggaaaagaa gatgaatgct
    14521 ttgcaatttc atagaaagaa gttgttattt attaatcatt taaaaaaaac agtaactaga
    14581 ccttatttct atatattatc gggcaat
//
LOCUS       DAZULY010000052        14259 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000052 DAZULY010000000
VERSION     DAZULY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.052"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            27..914
                     /gene="cdaA"
                     /locus_tag="ACG81A_001033"
     CDS             27..914
                     /gene="cdaA"
                     /locus_tag="ACG81A_001033"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFS5014417.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            911..2080
                     /locus_tag="ACG81A_001034"
     CDS             911..2080
                     /locus_tag="ACG81A_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFS5014418.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            2104..3459
                     /gene="glmM"
                     /locus_tag="ACG81A_001035"
     CDS             2104..3459
                     /gene="glmM"
                     /locus_tag="ACG81A_001035"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFS5014419.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(3531..5336)
                     /gene="glmS"
                     /locus_tag="ACG81A_001036"
     CDS             complement(3531..5336)
                     /gene="glmS"
                     /locus_tag="ACG81A_001036"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFS5014420.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            5745..6035
                     /locus_tag="ACG81A_001037"
     CDS             5745..6035
                     /locus_tag="ACG81A_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014421.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            6048..7073
                     /gene="sppA"
                     /locus_tag="ACG81A_001038"
     CDS             6048..7073
                     /gene="sppA"
                     /locus_tag="ACG81A_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS5014422.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            7076..7735
                     /locus_tag="ACG81A_001039"
     CDS             7076..7735
                     /locus_tag="ACG81A_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS5014423.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            7892..8824
                     /locus_tag="ACG81A_001040"
     CDS             7892..8824
                     /locus_tag="ACG81A_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014424.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            8978..10267
                     /gene="tig"
                     /locus_tag="ACG81A_001041"
     CDS             8978..10267
                     /gene="tig"
                     /locus_tag="ACG81A_001041"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFS5014425.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            10514..11764
                     /gene="clpX"
                     /locus_tag="ACG81A_001042"
     CDS             10514..11764
                     /gene="clpX"
                     /locus_tag="ACG81A_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFS5014426.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            11847..12434
                     /gene="yihA"
                     /locus_tag="ACG81A_001043"
     CDS             11847..12434
                     /gene="yihA"
                     /locus_tag="ACG81A_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFS5014427.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(12508..12663)
                     /locus_tag="ACG81A_001044"
     CDS             complement(12508..12663)
                     /locus_tag="ACG81A_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFS5014428.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            12799..14127
                     /locus_tag="ACG81A_001045"
     CDS             12799..14127
                     /locus_tag="ACG81A_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014429.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT         4681 a         2511 c         3065 g         4002 t
ORIGIN      
        1 atctcaaaag agaagagagg ctttttatgt catttcaaat cggggaactt tttaatttgg
       61 actattggca gcaaatgttt tccggtgact tgttctctct gaattttttg gtgaacatac
      121 ttgatattct ggttgtctgg tatcttgtat acaaactgat ccaactgctg cgaggcacaa
      181 aagcgattca gcttctgaaa ggtgtagcta tttttattgt tatccgaatc gtagcggaaa
      241 tcattggtct gcacaccttg tcttggctga tgaaccaagt cattacgtac ggggtgatag
      301 ctgctatcgt gatttttcag ccggaaatcc ggagaggact ggaacatctt ggacgcagtt
      361 cgcttttcaa acaatcaaaa agcgaacagc aggaagatga gaagatgatc ttgtcttttg
      421 acaaagccat ccaatatatg tccaagcgaa aaatcggtgc gttgatcact atcgaacgtc
      481 atactggatt agatgagtat attgagacag ggattgcttt agatgcagat atcactgggg
      541 agctgctgat caatatcttt attcctaata caccgctgca tgacggtgca gttattgtaa
      601 aagaaggaaa gatcgcagta gcaagcgcct atcttcctct atcagaaagt atgttgatcc
      661 ctaaagaatt cgggactcga caccgggcag ctgtcggtat aagcgaagtc agtgacgcga
      721 ttaccatcgt tgtctctgaa gaaaccgggg acgtcagcat cacgttagat aatgaactga
      781 tggctggctt atcccagcaa gaatatttgg caatcttgcg tagagaacta attgctgaag
      841 agaaaacaaa aaacaagaaa aacgtgattc aatctttcct agacggtatg actaagggag
      901 gggatcgcaa atgatctcaa aagagaaacg gacaagcatt ctttacagtt ttattgcatt
      961 gctgttcagt attgcgctgt acttcaatgc aaatggccag tccgttccaa atacgctttc
     1021 tggaaatgaa gcgtataatc aaacagttac gggggtgccg atcaagattt cttatgattc
     1081 aaagcgctac tatatccatg gatatgaaaa tgcagtgact gtcaaacttt ccagtgcaaa
     1141 ccgagttcag ttgaatgcag aggcaaacga agatacacgg atgttccgcg tcacagcaga
     1201 tatcactaag ctgggcgaag gaacacatga agttccgcta aaaattcaaa atttgagcag
     1261 tgctgtcaca gcaaagatcg aaccatcgac tattacagta acggttgaga aaaaggtaat
     1321 aaaagatttc gatgtggaaa cgttgattcc ttcaacgttc acaccatctg gatatgaact
     1381 tgatgatact tccgttagtc ctaaaaaagt ctctatcact acaggtgaca aaacattggc
     1441 agaaatcaaa cgagtagtag ctgatgtgga cgcttcgatg gtcacagatg atgggattaa
     1501 tagtgaggtc ccaatacagg cgctgaatgc agcaggggaa gtattaagta tcgtctctga
     1561 tccagtgcaa gtaacagtga gagctgattc gatcaagcca agcaagtcgg tacgacttta
     1621 tgggatacag caaggaacac cagctgccgg ggtagaaagc tatgatttca gtttttcaaa
     1681 actggaagca gaagtaagcg gctctagcga tttgcttgca tcgatcggtg acagtattgc
     1741 tgtaccgatc aatgtctcag gtatcaatca tcgaacaact cgtaaaattg aaataccagt
     1801 agaagagggg ttaagtatca agcccgaatc agttagtgtg gagattacac ccgtgttgca
     1861 aacgtcaagc agtagctctg cttcgacgag cagtcggaca caagaaaata caagtacgag
     1921 tagaagtatt tcatctgctg attcaggaac ggaaccatcg acaacaacca tttcttccag
     1981 ttcggaatct gggacaagca gctcgaccac aagtacagaa gaaacatcag aaacaagcga
     2041 atcaacacaa atgagcagaa caaattcctc agaaaattaa gttaaaggag taattttaca
     2101 actatgggta aatattttgg aacagacggc gtacgcggtg aagccaacaa ggaacttacg
     2161 ccagaacttg catttaagct gggacggtgc ggaggttatg tcctaagcca gcatgaagaa
     2221 gaaggaaaac gcccacgcgt gttagtaggg cgggatacaa gaatctcagg tcaaatgtta
     2281 gaagaagcgc tgatttcagg acttttatcg gtgggtatcg aagtgttcca gctaggtgtg
     2341 atctctacac caggtgttgc ttatctgaca cgtttgcaaa aagcaagcgc aggggtcatg
     2401 atttctgctt ctcataatcc agcacaagat aatggaatca aatttttcgg tgcagatggt
     2461 tttaaactag tagatgacca agagctagaa attgaagcat tattagatgc tgaagtggat
     2521 gaattgcctc gtccatcagc tgaaggattg ggaactgtag aagaatttcc agaaggctta
     2581 ttgaaatatt ctcaattcct tgtacaaacg atcaacggtg acctttccgg tttgaccatt
     2641 tgtgtcgatg cagcaaacgg agcaacttca acaagtgtta atcgtttgtt tgccgatctt
     2701 gaaacagatt tttatacaat gggcacaaat ccagacgggt taaatatcaa tgacggcgta
     2761 ggctctaccc atccagaacg tttagctgag atggtcgttg aaaaaggagc agatgctgga
     2821 ctagctttcg atggcgatgg cgatcgtatt attgcggtgg atgaacttgg aaacattgtc
     2881 gacggtgata aaatcatgtt tatctgtgct aaatacttgg cagaaaaaaa tcgattgaaa
     2941 aaagatacga tcgttacgac tgtcatgagt aacttaggat tccacaaagc ggtggaagga
     3001 atcggtttga aagatgtcgt tacacaagtt ggtgatcgtt acgttgtaga agaaatgcgc
     3061 aaaaacgatt ataactttgg cggcgagcag tctgggcaca tgattttctt agattttaac
     3121 acaactggag atgggatgct ttcaggaatt cagttactga atatcatgaa acagacaggt
     3181 aagaaattat cagagcttgc agcagaggta acaatctatc cgcaaaaatt agtgaatatc
     3241 cgcgtaagtg ataaaaacgg tgcgatggat gttccggcaa tcaaaaaagt gattgaagag
     3301 gcagaagctg aaatgggatc agaaggaaga atcctagttc gtccttccgg tacagaacca
     3361 ttacttcgag tgatggcaga agcgcctact gatgagaaag ttaattacta tgtcgataaa
     3421 atcgcggcag tagtcaaaga agaaatcgga ctggattaag agatattttc ataaaaaata
     3481 aaagaacaag gaccagtgtc agatgtctga cactggtcct tgttctttta ttattctact
     3541 gtcactgatt tcgctaaatt acgtggtttg tcaacgtcga atccccgtaa tagcgttgcg
     3601 taatatgcaa gtaattgagt cggtacgact gtggttagag tagttagata agggtggaca
     3661 gttggtagca ccagctggtc actttccttt tctaatccct ctgagacaat aactagtgtt
     3721 ttagcgccac ggctttcgac ttctttcagg tttcctcgag tatgcgcacc agtcacttct
     3781 tcactgatga ttccgatcac tggtgttcct tcttcgatca aagcaatcgt tccatgtttc
     3841 aattcgccag cggcaaaacc ttctgcctgg atataactga tttctttcaa tttcaaagca
     3901 gcttcaagag aaacgttgta gtcagcacca cgtccaatgt aaaaggcatt tcttgtatcg
     3961 ctgagatata cttctgccaa ctcttctaaa taatcttttt gggcgatgac cgattccatc
     4021 gcattagcaa ctaaactcaa ttcatgagtg atatcgaata caagagaagt ttcaaattct
     4081 tttttatcac cgatagcttt ggctaataaa gttagtacag ctatttgtgc agtataagct
     4141 ttcgttgatg ctactgcgat ttcgggtcca gcatgcaata gcagtgtaaa ggatgcttcc
     4201 cgcgaaagag tagaaccagc aacatttgta atcgtaagtg aagggtagcc gagacggttg
     4261 atttttacca gcacttggcg gctgtctgct gtttcgccac tttgactcaa gaaaataaag
     4321 aatggttttt ttgaaagaag aggtgtgtta tagccaaatt cactggctaa atgaacctca
     4381 acaggaatct gtgtcaattt ttccaaagtc tgtttacctg ctaaccccgc atggtaacta
     4441 gttccgcaag caacgatata gattcgatcg ctatcagaca atgtatcgac cagcttttga
     4501 tcaagagaga cacgattgtc ttctgttacg tatttttgag cgatacgacg catgataata
     4561 ggctgttcgt cgatttcttt cagcatataa aaaggatagg tacctttctc gatatctgaa
     4621 gcatctactt gtgcttcgaa tgaagaacgt tctacgatgt cacctgcaaa attttgaata
     4681 ataacttttt ctgatgtgac ggtaaccatc tcttcatctt caatctcaac gaattccttc
     4741 gtatgagcaa gcatagccat tgcatcgctg gcaatcacat tgaatccatc gccgagtccg
     4801 attaatagtg gacttttatt tttagcgacg taaataacat caggtgctgt ccgatcaatc
     4861 aaagcgaaag cgtaagaacc tttgatgacc cgcaaagctt ttaaaaacgc ctcttgcgtt
     4921 gtagtatcct tcgcgaaagt ttcaattaga tgagcgataa tctcagtatc ggtctcacca
     4981 ataaagtggt catttgctaa ataagcaatt ttaagttctt caaagttttc gattacgccg
     5041 ttgtgaacga gcacgaagcg tccactttgg gaagtatgtg gatgggcgtt ttcttcagaa
     5101 ggttgcccgt gagttgccca tcgtgtatga ccaataccta tatttcctgt agtatcaata
     5161 tttattttgt ttttcaaatt ttgaattctg ccttgtgctt tgaccagata atcagttgtg
     5221 ccatctgaga caaagatacc agctgaatca taaccacggt attctaattt ttctagaccg
     5281 tcaatcaatc ctggtgttac attttttaat ccaatcattc caacaattcc gcacataaaa
     5341 aacatcctac tttctataat gaattcgtat gaatgcttcc agctgcagga aaatcctttt
     5401 gttttatctt tgcagacatt ttttgtggat tttctgtaga gtgcagtccc tagggccatc
     5461 cgccgaatct ttcgataaac cctctcctcg tcatctgttt acacagacct ggcgctatcc
     5521 taaaaggtat aactatcctc ctttttgttt tttatctgga aacatttaaa atcataacct
     5581 tttgcgaatg ttcttgtcaa caaaaaaaga ataaaaaata aattggtata tatcaaattc
     5641 tttgtcaaat gtccttttta tgtcaaaaaa taatgggttt ttatctatca tctttttcag
     5701 gagttcggta caatagaaag taatcggata gaggaggagt aaaaatggat tcaaaaattg
     5761 agatcatgac actgggaatg ttaaaaaaac aattgtccaa attcgaagca tcagctggtg
     5821 tatcagatga tacgaaaata tttttagata ctggctggga tagcatccaa gaaatcgcac
     5881 ctgatgcctt ggaagttgct caagctcgtg agtttacggt agaagatgaa ttaacaaaag
     5941 agtcgttttc cgggtatgcg cgggaagaaa aagcagagcg gttcgacact tctgagcagt
     6001 cagaaactgt gatcgtcata aaaaacttgt attagaaagg agaaagaatg aataaaagac
     6061 gttgggcagc agttctgatt gctgcaggat tatttgtggt ttcgttagtt tcagctagtt
     6121 tgactaagcc tcaggaaaac gatcaaattg agggattgaa cagttggtta tatggggatg
     6181 aagaactagc tccaaatgtg ttggaagagg gagacagtac gcaaaaaatc gtaaaattat
     6241 cagtagatgg aacgattgct gcgggagggt cttcaggttt atttgcttct gaaggttaca
     6301 atcataaaaa ttttatggaa caactgaagg cgattgaaga agatccaaca gtcaaaggta
     6361 tctttttaga agtaaattct cctggtggcg gtgtctacga aagtgcggaa atcgctaaga
     6421 aattggatac gattcgtaaa gaacatgata tcccaatgta tgtcagcatg aaaaacatgg
     6481 cagctagcgg tggctactat atttctgctc aagcagataa aatctttgca acagaagaaa
     6541 cagtgaccgg ctcaattggt gtgatcatgt caggattgaa ttattcagga cttctagaaa
     6601 aactgggtgt ggaagataca acagttaaaa gcggagcgtt gaaagatatg ggttcaggaa
     6661 cgcgaccaga gacaaaagaa gaagaagcgg tgcttcaagc atatatcgac agcgcatata
     6721 gtcgttttgt agcaatcgtc agcgagggac ggaataaatc tgaagaagaa gtcaaaaaaa
     6781 ttgctgatgg aaggatttat gatggtgtcc aagcaaaaga agtaggttta gtcgatgaat
     6841 tgggatttcc tgaagatgct ttaaaagcta tacgaaagga acaacagctt gaagatgcgg
     6901 aactagtaga atattcttcc acgccatctg ggtttgctag tacatggtta ggaagtaaac
     6961 tagcagaatt ccagggattg aagccttctg agactagtca gatcttatcc gttcttgaga
     7021 atctagggac agcggaatca ccaaaagcaa tgtattatta cggaggtgag taagcatgtc
     7081 agagctttca aatgaaaata aggaacaaga aaaagataag caacagcagc ctgctgtagg
     7141 acctgactcc aacttttccc aaaaggtact gagctctttt gaaagagagc ctttgacgcc
     7201 cgaagaaatc aaaaagaaac aacgtaaatg gaaaaagtat acggacacaa gaaatgaacc
     7261 gccaaaagca aatgattttc ctaagtattt ctatgcaggt ttttttatcc gaatgtttgc
     7321 cttcttggtc gatcttttgt gtatcggagc tattacgcgt ttaacacttg gtattgccaa
     7381 aaatctaggt tggcttaatt tttcagattc ttatctgagt ctctacggat tcacagcctt
     7441 acttatttat ctagcgtatt ttattttatt gacgaaattc aatcatggcc aaacgattgg
     7501 gaaaatgatc tttggtattc gagtcatcag ctttgatgaa gaagaactta gctggtcaac
     7561 cgttttagtt agagaaggtg cttgccggtt cattctaaaa tttcctttgc tgatgttagg
     7621 gtatcttcca gcagtcttta atcagaaaaa gcaacatctg ggcgattttt tcactaatac
     7681 aagtgttgtt acgatcaatc tgatcaaagc gttcaaccag gaagtcaaca gctgatgagc
     7741 aataaaaagc agtaacagtt gaaagcacct tctaaaagtc agatctaaaa tctagctttt
     7801 agagggtgct tcagttattt ttaattgtca tgcttttttt gttttgctac tattgaacat
     7861 gagctgaata tgagcaagag gaggacactg cgtgaagccg attgattttc cagaaaaata
     7921 tgagaattta atgcgagtgg cacaacaagc actagcaaac cagcaatatc agcaggcaaa
     7981 agaattattt caacgagctt acgagttgaa agaaagcttt gaggctaata gcctgcttgt
     8041 tttttgtctt tacgagttgg acgagaaaaa agaagcattg aagcaagcac tcttgcatga
     8101 aaaacagtat cttcaaaatg aggagtttgc tgaattttat tttgacttgt taatcagtac
     8161 acaagatttt ttatatgcta gaaaattgat agcaagtaca gatttttatg aagctttcga
     8221 gcagcgggtc attgaaaaaa tccaatttgc agaagaatta tccggacaaa tggagaggca
     8281 gaagatcaag gcactccaca ataaatcgga ggaacttcct tcgttggaac ctgcacgtca
     8341 gcttgcgtct attgatgaga tcgaacagct tccttatcat gagtttatcc aaacagcgag
     8401 taaacttatt gtatcgccag aggtccatat tttagcaagg gcaaaattac tggagacact
     8461 taggcaattg aacgaatgcc atcctgtttt ctatttgacg attgaagaaa agttagtcaa
     8521 agtcatacca aaagatctgc caaagccgca gcagcaggca agttaccagc aattatgtgt
     8581 attttcagat cgttacggga atgaagatgc gctgcttagt tccgtgttaa aagaagaatt
     8641 cacgttgcaa tctgcaattg tttatcctgt ctatgataca tacatcgaag acccgaagcg
     8701 ctggtttcaa ctgactgtag aggcgtgtac aggaaaaacg atgtatgaag gtacagaaga
     8761 tgaaaaacaa gattttttaa aaaaaagaga aaaaattttg cagcaaatga tcttcttcca
     8821 ttagatttta tcagtgaaaa agataaaaat gttatgaaaa acaaggattc tatccattca
     8881 atatgtttac aatttgaaag ggttagtgta ctatttaaag gtatgcatga aacgtttttg
     8941 atgtataaaa tgaatttcta aactcggagg gaattaaatg actgcaacat ttgaaaaaaa
     9001 aggcacgaat gatggtgttt taaaatttac gattgcgcaa gacgaaatcc aaaaaggatt
     9061 aacaactgcg tttaataaag tgaaaaaaag cttgaacgta cctggattcc gtaaagggaa
     9121 agtttctcgt caagtattca accgtatgta cggtgaagaa gctttatatg aagatgcatt
     9181 gaatgctgta ttaccagaag cttatgaagc tgctgtcaaa gaagctggca tcgatcctgt
     9241 ttctcaacca aaaatcgatg ttgaaagcat ggaaaaaggt caagactggg taatcactgc
     9301 tgaagtaaca gtgaaaccag aagtaaaatt aggacaatat aaagatctag ttgttgaaaa
     9361 acaagaccgt gaagtaacag atgctgacgt ggatgcacgt atcaaacgtg aacaagaagc
     9421 acaagctgaa ttagttatca aagaagatgc tgcagctgaa aatggcgata cagtagtaat
     9481 cgacttcgaa ggctttgtag atggcgaagc gtttgatggc ggaaaaggcg aaaattactc
     9541 acttgaatta ggttcaggct cattcattcc aggttttgaa gatcaattag ttggtcataa
     9601 atcaggtgaa agtctagatg taaatgtaac tttcccagaa gattatcaag cagaagatct
     9661 tgctggaaaa gaagcagtct tcaaagtgac aatccatgaa gtaaaagcaa aagaagtgcc
     9721 tgaattagat gacgaattcg caaaagatgt tgatgacgaa gtatcaacat tagacgaatt
     9781 gaaagaaaaa tatcgcaaag aattagtaga aacaaaagaa aaagcagcgg acgatgcaaa
     9841 agatgaagca gcgatccgta tggctgttga aaacgcagaa atcgttgaat tgccacacgt
     9901 gatggttcat gatgaagtac atcgttcaat ggacgaattc ttgaacaaca tgcaacgtca
     9961 aggtatctct cctgaaatgt actatcaatt aactggttct acagaagaag acttgcataa
    10021 acaattcgaa ggtgaagcag aaatgcgcac aagaacaaat cttgtgatcg aagcaattgc
    10081 agctgctgaa aatcttcaag caacagaaga agaaatcaat gcagaaatcc aagaattatc
    10141 agaaacatac aacatgccaa tcgaacaaat caaacgcgtg ttgacagaag atatgctgga
    10201 acatgatgtg acaatgaaaa aagcaatcga attgatcaca ggtacagcga cagaagcgaa
    10261 agaatagaaa tcaagtaata aatcatttct gaagaagcta cttctagaaa attgtttatt
    10321 cgaaataagg gactgtgaca cgattttgtc acagttcctt attttttgtt aagagtaaaa
    10381 aatgcaaagg tataggtcag ttgatccttt gttaaacact ttttcttttt actaaactat
    10441 gttatgatag taaaagactg taaagtcttt gatagtttct tgcatacttt ttgcaagtta
    10501 aggggtgaag accatgtatg acaatccaag tagcaatgag accgtacgct gttctttttg
    10561 cgggaaaacc caagaagaag tgaaaaagat cgtagctggt ccaggggtct acatctgtaa
    10621 tgaatgtatc gacctatgta aagaaatcat tgatgaagaa ttttatgaag aagctgtacg
    10681 cgaattcact gaagtcccta aaccactaga aattcttgaa gtatttaata actacgtgat
    10741 tggacaagat cgcgcaaaac gctcgttagc tgttgccgta tacaatcact ataaacgtgt
    10801 caatcagcaa gcacaagaaa acacagatga tgttgaactg caaaaaagca atatctgctt
    10861 gatcggacct acagggtctg gtaaaacatt tttagcacaa acattagcaa aaacattgaa
    10921 tgtacctttt gcaatcgcag atgcgacaag tttgacagaa gctggttatg tgggggaaga
    10981 tgtagaaaac attttgctta aattgcttca atctgctgat tacaatgtgg aacttgcaga
    11041 aaaagggatc atctatattg atgagatcga taaaattgcg cgtaaaagcg aaaatgtatc
    11101 tattactcgt gacgtttccg gcgaaggtgt ccaacaagcc ttgctgaaaa ttctagaagg
    11161 aactgttgca agtgtgccac ctcagggcgg acgtaaacat ccacatcaag aatttattca
    11221 gattgatacg acgaacatat tgttcatcgt aggtggtgca tttgatggca tcgaaacgat
    11281 tgtgaagaat cgtatgggcg aaaagacgat tggatttggt acacaaaacc aaaaactttc
    11341 tgaagacgaa agtgtcatgc agcatatcat tcctgaagat ttactaaaat tcggattgat
    11401 ccctgagttt atcggacgat tacctgttat ggcagcttta gagaaattga caacagatga
    11461 tctggtacgg atcttgactg agccgaaaaa tgcgctagtc aaacaatatc aaaaacttct
    11521 ttctctagat aataccgaac ttgaattcga acctgaagcg ttgagagcca ttgcgaaaaa
    11581 agcaatcgaa cgcaataccg gtgctcgagg acttcgttcc atcattgaag aaatcatgat
    11641 ggatgtgatg tttgatatcc cttcaaatga aaacatcgaa aaagtgatta tcacaaaaga
    11701 agcagctgaa ttgtctggtg agccaacagt aatctataaa gattcagaga aaaaagcggg
    11761 ttgatgaaaa gaggctgccg taaaagtcat tttggctttt gcgacagccg cttgctttat
    11821 tcatcacctt gttaaggaga gagaccatga aagtccatca agctgaaatt atcataagtg
    11881 cagtggcacc aaaacaatat ccagatacac aattgccaga aatagctctg gcaggacgat
    11941 caaatgtagg gaaatcttct tttatcaaca cattgattga tcgaaagaat ttagcccgta
    12001 cttccggtaa gccaggaaag acccagacat tgaattttta tttgatcgag aacgcattac
    12061 attttgtcga tgttccggga tatgggtatg caaaagtatc caaaactgag cgagcaaaat
    12121 ggggaaagat gatcgagaca tatttaacga ctagagacca gctacgtgcg gtcgtttctt
    12181 tagtagattt gcgtcataag ccttctcaag aagatatcca gatgtatcaa tttttgaagt
    12241 attatgatat ccctgtaatt gttgttgcca ctaaggcaga taagatccct cgtggtaaat
    12301 ggaacaagca tgagagcgaa atcaaaaaag cgttggattt tgatcgcaat gacacgttta
    12361 ttctcttttc ttccgttaca aaacagggaa aagaagaagc gtgggcagca atcgaacaag
    12421 caattgaatc ataaaaagaa taaaaaagac ttaccagtca aactctttca taaaagtttg
    12481 gctggtaagt ctttttttga gcttaactta cttttgtttc ttgtcttttt tcttttcatt
    12541 ttgtggctgt ggttttagaa atcgatcgat ctcttctttt ggtgcaactg ttttttccgg
    12601 atcgacagca aatgattcga aaagtttatc taaattatct gtacgttcaa atttcaaccc
    12661 cattgtaaac acctctttgt ttctttttac ttagtctatc atgtaatcgt ccaaattggt
    12721 ttctaagaca taaagtttta gtttttcttt atcattggtt ataattaaat tataaaaaga
    12781 tttgaaagag gtgagaaaat gattggcgaa gataaattat tgatgactat ccagctcaca
    12841 gacttaaatg aagaagaaaa aaaacaggaa ataagcgatt gggctatttt gacacgtctg
    12901 ttgatccagc caacatttat ccgctctttc acacaacaag cggatccgta tgatcttcga
    12961 aagcttcaag aaaaaataat gctagcatcg gtaagagacg aaagttggtt agttgtagga
    13021 aatgacgaga acgaatgtag ttttcgcttg gaagataatc aattattgat aaaaaatgta
    13081 ttgtctatct ctgttttcaa agaaaagcaa tttttgattc gagacttcat tcagaaaaaa
    13141 atgattgctc atggggtgtt cgcctatatg cgagcttata gcgagtttat ctatcacaat
    13201 acaaaacaga tctctcaacg gctattgttt gagaaaaagg atgaaatcga acatttacct
    13261 aaaatgaaac aacaaaacgg ggaagtagta gtcgattgca accaatttcc aggatacgat
    13321 cttttttacc aaggtttatg tttcacttcg tgttgggaaa tgtattatag tcgctattat
    13381 catcaaatca tccctaaacc gatttttctc gacatccaac aagtagaaaa agtgaaggaa
    13441 ttagaaaacg aagtcatatg tatccagctt tatcgagacc cctttaactg gcaaaagcca
    13501 aacaatcaaa tgtttcaatc ctattttagg gaccagcttg ggtttgatca tcttgcttgg
    13561 gacaatggtg tcggtttatt gaaaccgcca ttcgttgaat acatttacac tgaccatacg
    13621 atccaatctg tccagtatca gaatgcacaa atgcaacctg ttcctaaaaa gaatgcttca
    13681 ttttttgtaa caaaaagtta tgatatccaa caaggagatt ataaagaaag gcgtgttcgc
    13741 ggaacactaa atgctcaggc gtattttcct tgggttgacg agaatcgatc acgcatgatg
    13801 tgctataaag ttatcgatcc gactgttgct ttggataatg gaatcgaagc ttattgttat
    13861 tatattaggg aatatctaga agtagaggta acagatgaaa aatatcaaga gtatctttta
    13921 tccttaagaa tctatgttcc ttcagaatcc ctgtcagaat taccgttaaa ggaaatcaaa
    13981 catagactct ctgatgttac cttcaaacga ataaagaaaa agcggcgaag tatccaattc
    14041 gatgtcaaga aaggtgagca gcatctgcgt gtgcaatttt tagattaccg agaattagaa
    14101 caattgatta ctttacaaaa aatatgaggt gaagaaaatg gataaacaac tctattatcg
    14161 aataatggtt ccggcatcat tattatccgt ctgtctcttt tttgttccta attttcctat
    14221 tcgatcagtc atcggttttt tggtcatgtt tggcgggat
//
LOCUS       DAZULY010000053        14235 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000053 DAZULY010000000
VERSION     DAZULY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.053"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(147..3800)
                     /gene="rpoC"
                     /locus_tag="ACG81A_001046"
     CDS             complement(147..3800)
                     /gene="rpoC"
                     /locus_tag="ACG81A_001046"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFS5014430.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3879..7496)
                     /gene="rpoB"
                     /locus_tag="ACG81A_001047"
     CDS             complement(3879..7496)
                     /gene="rpoB"
                     /locus_tag="ACG81A_001047"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS5014431.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8224..9192
                     /locus_tag="ACG81A_001048"
     CDS             8224..9192
                     /locus_tag="ACG81A_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5014432.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9210..10115
                     /locus_tag="ACG81A_001049"
     CDS             9210..10115
                     /locus_tag="ACG81A_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5014433.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10112..10918
                     /locus_tag="ACG81A_001050"
     CDS             10112..10918
                     /locus_tag="ACG81A_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5014434.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            11075..12043
                     /locus_tag="ACG81A_001051"
     CDS             11075..12043
                     /locus_tag="ACG81A_001051"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFS5014435.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12360..12884)
                     /locus_tag="ACG81A_001052"
     CDS             complement(12360..12884)
                     /locus_tag="ACG81A_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFS5014436.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13171..>14235
                     /gene="scm"
                     /locus_tag="ACG81A_001053"
     CDS             13171..>14235
                     /gene="scm"
                     /locus_tag="ACG81A_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFS5014437.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         4035 a         3068 c         2804 g         4328 t
ORIGIN      
        1 aagatgatcg ttccgatacc ttaattggat acgaaccgat gttgttaaga tcctttccca
       61 cttttcttct agttagacaa attattttag acaaaccaaa aaagaggttg tgacaataat
      121 ttgtcacaac ctctttttta aaatatctat tttgtttgat tgttcattgc gtcttctgca
      181 gccatttgcg cttcaatatc tgaaatactg tagacatttt cgcttgctac accgacttct
      241 tttggttcca tgtcacgata acgttgcata ccagtaccag ctgggatgat cttacctata
      301 ataacatttt ctttcaaacc aagaagagga tctttcttac cgcggatcgc agcatccgtc
      361 aagacacgag ttgtttcttg gaatgatgca gcagataaga aactatttgt ttctaatgat
      421 gcttttgtga tacctagtaa gacaggtcga gaagttgcag gaacgcctcc tgagactagc
      481 gtattgtagt tttgatcttt aaagtctgcg atgtctaata atgtacctgg taagatatct
      541 gtatcacctg gatccattac acgaacttta cgcagcattt gacgaaccat tacttcgata
      601 tgcttgtcgc cgatttctac cccttgcata cggtaaacac gttgtacttc acgaagcaag
      661 tagttttcaa cagataatac atcgcgaact tgcaacaatt gttttggatc gattgatcct
      721 tctgtcaatg gtgcaccacg gtggatcata tcgccttctg ctactttcat acgtgctgta
      781 taaggaacag tatacgtacg tgtatctgtt tttcctttga ttgttacttc tttggtacgt
      841 gttgcaggat cttctgagat atcgatgaca tcccctgtta cttctgtgat cactgcttgc
      901 cctttcggat tacgtgcttc aaagatttct tggacacgag gaagaccttg agtgatatcg
      961 tctccggcaa ccccacctgt atggaatgta cgcatcgtca actgagtacc aggttcaccg
     1021 attgattgag cggcgatcgt accaactgct tctccgactt caacgtcaga gccagttgca
     1081 aggttgcgtc cgtaacaatg tttgcagaca ccatgttttg tattacatgt gaagacagaa
     1141 cggattgtca ctgtttcgac acctgcatca acgatttgtt ttgcgatgtc ttcggaaatc
     1201 aattggtttc ctgcaatgat caattcattt gtttcaggat gacgtacttc tttacgggtg
     1261 taacgtccca ctaaacgatc ttctaatgat tcgatgattt catttccctc gcggatggct
     1321 tggatttcaa gacctcggtc agtaccgcaa tcttcttcac ggatgattac atcttgggcc
     1381 acgtcaacca gacgacgagt caagtaacct gaatcggcag tcttcaaggc tgtatcggtc
     1441 atccctttac gagccccgtg agttgagata aacatttcca agacggatag tccttcacgg
     1501 aagttcgaaa tgattggcaa ttccatgatt cgaccattag gagcggccat caaaccacgc
     1561 attccggcaa gctgtgtaaa gttggaaatg ttaccacgag ctccagaatc agacatcatg
     1621 aagattggat ttttcgcttc caaaccttcc atcagttttt gttggatgct gtcttttgca
     1681 gcattccaaa cagcaatgac acgttcataa cgttcgtcat ctgtgatcag accacgacgg
     1741 aattgttttg taatagtttc aacttgttta tgggcatttt caatgatttc ttgtttttca
     1801 ttcagtacac tgatgtcagc gatccctact gtgataccgg catgtgtcga atgtttgtac
     1861 cctaagtctt tcatacggtc aagcatctta gaagtttctg ttactttgaa tcgtttgaag
     1921 acttctgcga tgatgtttcc taagtttttc ttcttgaacg gtaatactaa ttcttgctct
     1981 ttgatatgtg caggaatatc agtaccagct tcaacaaagt atttgtccgg tgtttggacg
     2041 gtcaagttga agtctgttgg ttcattcaga taagggaatt caggaggcat gatctcgttg
     2101 aagatgatct taccaactgt tgtgatcatc gtacgttctt tttgccattc agtaaatggt
     2161 ttttcaccta atgagttagg gttcacaccg atacgtgaat gtaaatgaac atatccgttg
     2221 cgccatgcga taacggcttc gttttgatct ctgaagacca ttccttcgcc ttcgcgtcct
     2281 tcttcttcca ttgtcaagta gtagttacct agaaccatat cttgagaagg agtaacaact
     2341 ggtttaccat ctttagggtt caagatgttt tgagcagcta acatcaacat acgtgcttct
     2401 gcttgtgctt cttcattcaa tggaacgtgg acagccattt ggtctccatc gaaatcggca
     2461 ttataagctt cacatacaag tggatgcaaa cggattgcgc gtccttgtac taaaactggt
     2521 tcgaatgctt ggataccaag tcggtgaagc gtaggtgccc ggttcagcaa tactgggtgt
     2581 tccttgatta cgtcttctaa aacatcccat acttcgtctt cttgacgttc gattttacgt
     2641 ttcgcatttt tgatgtttga agctagttcg cgttgaacaa gctcacgcat cacgaatggt
     2701 ttgaataatt caatggccat ttcttttgga agtccacatt ggtacatctt caagaaaggt
     2761 ccaacaacga taacggaacg acctgagtag tcgacacgtt tacctagcaa gttttgacgg
     2821 aaacgacctt gtttcccttt caacatatga gaaagagatt tcaatgggcg gttacctggt
     2881 ccagtaactg gacgtccgcg acgaccgtta tcgatcaatg catctactgc ttcttgcagc
     2941 atacgttttt cattttgaac gatgattcca ggtgcattca agtctaacaa acgtttcaaa
     3001 cggttattac ggttgattac acgacggtaa agatcattca agtcacttgt tgcaaaacgt
     3061 ccaccttcta attgaaccat tgggcgtaaa tctggtggaa taacagggat aacgtccata
     3121 accatccagc ttggttgatt tcctgaagcg cggaatgctt ctaaaatatc caaacggcgg
     3181 attgcacgtg tacgtttttg tccttgtgca gatttcaatt cttctttcaa ttcggctgct
     3241 tctgcatcta gatcaacatt atcaagtaat tgtttaatcg cttctgcacc catagcagct
     3301 tggaattctt gtccatattg ttcacgtttt tcgcgatatt cgcgttctgt caacaattgt
     3361 tttttctcta aattcgtatt tcctggttcg atgactacat atgaagcaaa ataaatgatt
     3421 tcttccaaag cgcgtgggct catgtctaat acaagaccca tacgtgaagg aatcccttta
     3481 aagtaccaga tatgtgaaac aggagctgct aattcaatat gtcccatgcg ttcacggcga
     3541 actttcgaac gagtaacttc cactccgcat cgatcacaaa cgattccttt ataacggata
     3601 cgtttgtatt ttccgcaggc acattcccag tcttttgtag gaccaaaaat ccgctcacag
     3661 aacaaccctt cacgttcagg ttttaaggta cgatagttaa tcgtctcagg tttttttact
     3721 tcaccgtaag accagcttct gattttttcg ggagaagcca aacctatttg catactttcg
     3781 aatttattta catcgatcaa aaggggttcc ctccatttca tttaatggca aaccgcaaga
     3841 cggctgatct tcgaccgcct tgcagctcat agaccggatt agtctaaatt atcttctgcg
     3901 gtttcaaagt tttggatgac atcgtctttt tcatcttcca cttgttcagc agctttcttt
     3961 tctaattctt tggcagtttg ctgttcggca aattttgtca aagcatcaac agtgatcaaa
     4021 tcgtcgtctt catcgtccat gtcgcgaagt tcgatctctg tttcttcgat atccaagaca
     4081 cgcatgtcca gacctagtga ttgcaattct ttcaccaata cgcggaatga ttccggtaca
     4141 cctggttttg gaattggttc acccttgacg attgcttcgt atgttttcac acgtccgact
     4201 acgtcatctg atttgtatgt caagatttct tgtaaagtat aagccgcacc gtaagcttcc
     4261 agtgcccata cttccatttc accaaaacgt tgtccaccaa attgagcttt acctcccaat
     4321 ggttgttgtg taaccaatga gtaaggtccg attgaacgtg catgcaattt gtcatcgacc
     4381 atgtgagcaa gtttgatcat gtacatcaca ccgacagaca cacgtccatc aaatggttca
     4441 cctgtacgtc cgtcgtacag aacagttttc gcatcgctgg ccataccggc ttcacgaact
     4501 gtttcccata cgtcttcatc acttgcacca tcaaatactg gcgttgctac gtggatacct
     4561 aactgacgtg cagccatacc caagtgtaat tccaatactt gcccgatgtt catacgtgaa
     4621 ggcaccccta aaggattcag catgatatcg attggtgttc catcaggtag gaatggcata
     4681 tcttcttccg gcataatacg ggatacaacc cctttatttc cgtgacgacc ggccatttta
     4741 tccccttcat ggattttacg tttttgaacg atataaacac gaactaacat gtttacgcct
     4801 ggtgacaatt catctccagc ttcacgagta aagattttca catcgtgaac gataccgccg
     4861 ccgccatgtg gtacacgtaa tgatgtatcg cggacttcac gagctttttc gccaaagatc
     4921 gcatgcaata gacgttcttc tgcagataat tctgttaccc cttttggcgt gactttccct
     4981 acaagcaaat cgccatcttg gacttctgca ccaatacgga tgatacccat ttcgtctaaa
     5041 tcttttaatg cgtcttcccc gacattaggg atttcgcgtg tgatttcttc aggtcctaat
     5101 tttgtatcgc gggcttctga ttcatattct tcaatatgga tagaagtgta tacgtcatct
     5161 tttaccaaac gacggctcat gatgatcgca tcttcatagt tgtacccttc ccaagtcatg
     5221 aaaccaacta aaacgttttg gcctagagcc atttcgcctt gttccataga tggtccgtct
     5281 gccagagtat cccctttttc gactttctca cccaaatgaa cgattggacg ttggttgtag
     5341 cttgttccgg agtttgaacg acggaatttt gttacatcgt atttatctaa tgcaccgttg
     5401 tcgcggcgta cgcggatttc agaagcatca acgtattcaa cgacaccgtc atgtttacat
     5461 aacaaagcag ctcctgagtc atgcgctgat ttgtattcca taccagtacc aacccatggt
     5521 gcttgaggat tgatcaacgg cactgcttga cgctgcatgt tggcacccat caaggcacgg
     5581 ttggagtcat cgttttccaa gaaaggaata catgctgtcg caacggcaac tacttgttta
     5641 ggtgatacgt ccatgtagtc tactttatca atagatactt ctaagttttc actttgagca
     5701 cgagccataa caacgtcttg agcaaatgtt ccatcttcat tcaatgggct gtttgcttgg
     5761 gctacgatgt aatggtcttc gatatcagct gtcaagtagt cgatttggtc agtaacacga
     5821 cctgtttcac ggtctacacg acgataaggt gtttcgataa atccgaattt atttactttc
     5881 gcataactgg acaaactatt gatcaacccg atgtttgggc cttcaggagt ttcgatcgga
     5941 cacatacggc catagtggga gtagtgaacg tcacgaactt cataaccggc gcggtcacga
     6001 gtcaatccgc caggtcctaa ggctgaaaga cgacgtttgt gtgtcaattc gccaagaggg
     6061 tttgtttgat ccatgaactg tgacaactga gaagaaccaa agaattcttt gatacttgcc
     6121 acaactggac ggatattgat caattgttga ggtgtcaatg tttcagtatc ttggatcgac
     6181 attctttcac gaaccacacg ttccatacgt gctagcccga tacggaattg gttttgtaaa
     6241 agttcaccga ctgaacggat acgacgattt cctaagtggt cgatatcatc gacattgccg
     6301 ataccttcca tcaagttcaa gaaatagctc attgaagcaa tgatgtcggc tggacgaacc
     6361 gtttttacag ctgcttctgg atagccgttt ccgatcacgt ttacttcacg ttcagggtct
     6421 cttggtgaaa atactttgat gacttggaca gtcattggat ctgtgaccac accgtcttct
     6481 gatggatagt aagtcactga atttaggcca ttatcaatga aaggagctag tgtttccatg
     6541 acttggtgag tcagaactgt tcctttttca acgatgatct cgcctgtttc tggatcaact
     6601 aatgtttcag ctaatgtcag attcaataga cgtgttttca aatctaattt tttgtttact
     6661 ttgtaacgac caacgtttgc taagtcgtaa cgttttggat cgaagaaacg agcattcagc
     6721 aagttacgtg agctgtctgc tgtttttggt tcgcctggac gcaaacgttc gtaaacgtct
     6781 ttcaaacctt cttctgttct tgagtcgctt gcgtttttat gcaagtcttt ttcgactgta
     6841 ttgcgtaatg tttcgctgtc gccgaagatt tcaaagatcg tatcatcgga accaaatcct
     6901 aacgcacgaa ctaatacggt taaaggaatc ttacgtgtac ggtcgatacg tacataagag
     6961 atgtcttttg catcagtttc catttctagc caagctccac ggttagggat aacagtagaa
     7021 ccgaatcctt cttttccgtt tttgtcgact tttccatgga aatagacacc tggtgaacgg
     7081 actaactgag aaacgattac tcgctctgcc ccgttgatga tgaatgttcc ttgttctgtc
     7141 attaatggga aatcaccgaa gaatacttct tgtgctttga tttcgccagt ctcgcggttt
     7201 gtcaaacgca atgttacatg taatggtgca gagtagtttg catcatgcgc acgagcttct
     7261 gctactgtat actttggttc tttcaattca taatctacaa attccaaaga tagattgcca
     7321 ttgaagtcat cgattggcaa aatgtcttca aacatttctc taagtccctc atctaagaac
     7381 cattggtaag agtctgtttg aatttcgatc aaatttggta attccaacac ttcactgatt
     7441 cttgcgaaac ttctacgttc gcgatgtttt ccgtatttta ctacgtgtcc agccaagctc
     7501 ttcacccctc taaaatgtca ttcaaaaaga ctacccgatc aattgttaca ataatatttc
     7561 aaatattatt tttaggtaac aacagacatt ttttgcggct tttaggcaaa aaaaaaccaa
     7621 aaatagaaca ttctcttgaa accttacttc gtgagcttct acttttgttt ccttatatta
     7681 aagccgaaac ggacaatatt tcgtttctgc cttttcttca tcagatagtt tttgcaactt
     7741 catatactac acttattttc tataaaagtc aacacttcct ggcgtttctt ctgctttttc
     7801 gcaacatttt cttaatgttt tcaattttat gttttcaatt atctgacaat tcttaattga
     7861 cagacctttt tatctcggtt atactttaga aaattcattt tgtggaggaa cttctatgcg
     7921 aaccaacatc aaccaaatcg caccaacaca actgaataac ttttacttta gctactttag
     7981 ataggtttgt attcatcatg atggatacaa acgttaaacg acagtttgta tctatgatgg
     8041 ctaaagaact ttgtattgca gcaaaaagcc acgtagacaa aatgctaagt ggctgagcag
     8101 tgaaaataca aagtaaaagt atcaagcatt ctactttttc aatttatttt ccgacttttc
     8161 aatccatttg gacattttgt ctgaatggat tttttgtttc ccaaaattaa ggaggaaaaa
     8221 gaaatgaaaa aaaatataat catcggatta ggcgctgccc tgctactagc tttaggaggt
     8281 tgcgcacaaa aagacaattc cgcagaggca tcagatggac aaaaaacaat tggcgtattg
     8341 caacttgtac aacataactc gttagatgct gcctataaag gattcaaaga aggattagca
     8401 gaagaagggt ataaagaagg cgataatcta acaatcgatt atcaaaatgc ccaaaacaat
     8461 caagacaacc tgaaaagtat gagtgagaaa ctcgtcaaag aagatcctga tttgctactt
     8521 ggaatcgcca ctccagcagc acaatcttta ttaaatgaaa caaccactat cccgatgact
     8581 gtcacagcgg tcacagactt ggaacaagcc catttagtca catccaatga aaaacctggc
     8641 ggcaatgtga ctggcacgac agatatggtg ccaatcgaaa aacaaatcgg cttactgtta
     8701 aatatcgtgc cagacgccaa aacaatcggc atcatgtata acaacggtga agcaaactct
     8761 aagatccaag ctgatttggc agaaaaagaa ttgaaaaaag cgggcgtcaa agtcaaagta
     8821 ctgacagcaa attccacaaa tgatgtccaa caagtgacga aaagtctagc taaagatgta
     8881 gatggaatct atattcctac tgataacacg ttcgcaagtg cggcaagtgt tgtcggtgaa
     8941 gtcgcaaaag aaacaaaaac acctgtcgtt gctggcgcag ttgatcaagt caaagacggc
     9001 ggtctagcaa cagtaggaat cgactacgaa agcttgggca aacaaacagg gatcatggca
     9061 gcaaaaatct tagacggcaa gaatccaagt gaacttcctg tcgaaacagc gaatgatttg
     9121 gaactgtatg tcaataaaga catggccaaa gcattaggca tcaatccaga cagcatcaaa
     9181 gaagctgaat aaaacacagg aggattctca tgacaaattt gctctctctt ttcttatcat
     9241 ctgcatcgca aggggtctta tgggccctac ttgcgattgg tgtatacgtc actttccgta
     9301 tcttggacat tgccgacttg actgctgaag gaagctttcc tttaggtgca gggatcgcgg
     9361 cagtaagtat tacaaatggc tatcatccat tgacagcttg tcttctaggc tttttagggg
     9421 gtgcggcagc agggcttgtt tccggactgc ttcacacaaa attgaaaatt cccgcccttc
     9481 ttgcagggat catcaccatg actggtttat attctgtcac atcccgtgtg atgggtgcgc
     9541 cgaacatctc tttattgggt cagccaacga tctttcattg ggtcgagtca ttcggcataa
     9601 caaaagacaa cgctgtactg atcgtcggtc tgctttttgc tttgatcgtt gtatgtttac
     9661 ttgttttgtt cttacgtaca gaaacaggac ttgctattcg tgcaactggc gataatctgg
     9721 caatgagcga agcaaacggg atcaacacag ataatatgaa gatcatcggt tatatgattt
     9781 ctaatggatg cattgctctt tctggctcct tacttgcgca aaacaacggc tttgctgatt
     9841 taaattctgg tattggaacg atcgtaattg gacttgcttc tattatcatc gcagaagttt
     9901 tgttacgtaa ccagccacta tttctccgtc tcttgactgt cgttttaggt gcggtcattt
     9961 atcgttttat tttagcgtta gtcttcgaac tgaatgttga accaagtgat tcgaaacttg
    10021 cttctgcttt cgtgctagtg atctgtcttt ctttcccgca gatccagcaa aaattccatt
    10081 tacctcaaaa aaagaaaggc ggtagtgtca aatgaatgac gtcttaacat tggaaaatct
    10141 ccatcatacc tttgaaaaag gaacgatcaa cgaaaatcat gtcctgaaag gcattaatct
    10201 ttccttagat cccggcgact tcgtgacgat catcggtgga aatggggctg gtaaatctac
    10261 tttgctgaac agcatcgccg gtacattttc tgtcgatcag ggtcaaattt tattgaatgg
    10321 aaaaaatatc acgaaaaaat ctgttgtcga acgttcgaaa tccatcagtc gcgtattcca
    10381 agatcctaaa ctagggacag ccgttcgttt aacggtagaa gaaaatcttg ctcttgctat
    10441 gaaacgagga aaaaaacgcg gcttcttccg tggtgtcaaa ccacaagacc gaacattttt
    10501 caaagaccag ctggttcggc taaatttggg gttggaaaat cgtttatcct cagaaatcgg
    10561 tttgctgtct ggaggacaac gtcaagctat tacgttattg atggccacat tacagcgtcc
    10621 agaactgatc ttattagatg aacacactgc agcgcttgac cctgctactt ccaaaacggt
    10681 gatggaattg acagaaaagc tgatcactga gcagaatctc actgctttta tggtcactca
    10741 tgacatggaa gacgcgatcc gctatgggag tcgactgatc atgctgcatc aaggaaggat
    10801 cgtcgtagat atccagcaag aggaaaaaca gcagctcaca gtgccccaat tattagcgtt
    10861 gttcaaacaa aacagcggca cggcattaaa agacgatcaa gtacttttat cttcataatc
    10921 agaaagagga tgggacaaaa aatgaacgcc caccctcttc ttttttctct ttccttcttt
    10981 cgtttttgct ttatgtatca accgttgttt cttcgaaaac tattttgttc tctccttctt
    11041 cctgatagaa tggatgggaa aggagcttga tgccatgaca acgaaagaca aggtacttca
    11101 attattgaaa aagacaccag attttctttc cggagaaaag ttggcacagc aactggaagt
    11161 ttcccggaca agtatttgga aagccatcaa agaattggaa aaggcaggct atcgttttga
    11221 gcacgggcct acagggtatc gttacctgcc ctctgacgta ctagatgcaa aagaaatcca
    11281 gcaagagcta cggtcgttta ttcctgattt gaaagtacaa acgatgttga cttctgaatc
    11341 tacgatgaaa gatgccaaac tggcagcagc tcaaggagat tctgtttcga agttatttgt
    11401 tgctgataca caagaacaag ctcgtggacg ttttggacgg ccttttttcg ctgaacctgg
    11461 acgaggaatc tacatgagtc tcttgctcca tccagacaaa cggtttgatg aattgcctca
    11521 atatacgatc atcgcagcca gtgcctgtgt gaaagccatt gaatctgtaa ccggtcgttc
    11581 tgcttcgatc aaatgggtca atgatatcta catggatgga aaaaaagtct gtgggatttt
    11641 atctgaagca gtcagtgata tggaaagcgg caggatcagt catatcatca ttgggatcgg
    11701 gcttaatttt tctgttcctc aaaatcattt tccagctgat ctccaagaaa aagccatctc
    11761 tgtctttccc gacggacaag ctagtgtcac aaggaatcag ctaatcagca aaatctggca
    11821 agaattgttc acttatcttt ctcacccaga aaaagcgttg gacttctata aagaaaaatc
    11881 attcgtattg ggaaaaaccg tgtcgttcaa tcaaaaagga acgacttatg tcggtcttgc
    11941 aaaagaaatc ggtgatcatg gcgaactgat cgtccaaaca aatgaacaga agatgagttt
    12001 gtcttctggc gaaatcagtc tcacatctat ccgtacgcaa taattatcag atttcacaaa
    12061 aggacacctg atctcataca gtgtcctttt ttctgaattc tcttcaaaag actctcctta
    12121 ttctatcagc tgacagtttc tctatcctct agaaaaaggt attctaaaaa tatctataaa
    12181 gcatggtaca ctttctttaa atgaatcaat gcaaaaaaca agagagtcat cagtttacac
    12241 gatcaaaaga gatagcaggc cgcttccttt ttcaatctta tagatagacc atgggtaacg
    12301 ataaaaacaa aaaagaactt gcgacttatt ttgccgtaag ttctttttta gctagtgcct
    12361 taatctctta atcccaacaa acgtctcgca tagatcacct gaccagagtg catagcagca
    12421 tcatcaataa tagaaacgag acgcattcct cgtgtgactg gtggtgtcca gttctcgtca
    12481 attacttcat ctagctcgga aggttccagc cttcttaagt aagaaatagc aaaatcagca
    12541 gctcctcgca agtatcctag cagatcttct atttgattag cttggatctg ctttgcttgt
    12601 tccagactat gcttccaatc ttgttcgtta tctgctatcg tcatcggaaa ccggctttcc
    12661 cagtcttgac tatgccaaat tggagaagtt cctgctaaat cagcaatctg gaaatctaat
    12721 tctcgagcgg tatgccaagc cagccaagtc attgatttga tctgcggggc tctatcttcc
    12781 actggaaaac gattggcctc ctcaatagag acgccttcaa acaaacgttc aaaccgatcg
    12841 tatgcccgat ccacactctc gcatagcata tctaaataat ccatttattt tcctcctttt
    12901 tatttatcct acttactttt tcctaattct aggataccta cttatatgtt actacacacc
    12961 cttatttttt acactttaac agtcaccatc atcaatagtt tggagattga aagaaaaaga
    13021 tagtgtcgaa ctatcaattg aacaagaagc aacaatatga acgtttcatt aatattcttt
    13081 aaataaacaa tgatttttca aacgaacagt ttaagactag gagaaaaaga gtataatagg
    13141 aaaatgtttg aaaggaaagg aaggggaaaa atgaaaaaaa gggcaagttt actagtccta
    13201 gttgctttgt tgttcaatat cttttcgtat ggtgtggccg tttcagcgga aacatacatt
    13261 cctaaagacc ctgggaatat ttataatgat ctaactggta actatggctt gttaggaatt
    13321 gctagtcaat tccacgtatt tgctaaaaat aagacaactg tgaatgcaca tacagacgga
    13381 aatatagcaa cacatgaact tgatgcaaaa aataattttg gtacagagct tcatgaaggt
    13441 ttattgaacc aagaaataaa ttatatacaa aaaactgatt ctttcgctaa ttcttcaggc
    13501 atacctacag gtagcacaag aatgaataaa tttgtcgttg gcagtaacgt ggatgtagca
    13561 cttcaagatg gtcatcctac agtcaatgga tccaggcttg atcacttaac tccagatgat
    13621 ttttataaag atcgtgctgg gcatgaatat attgatttcg agcaggaatt tgctaaattg
    13681 aatgtagcag caaatgatct tgcaacgatc actccagcta aaacatatac tgctgcagat
    13741 tttccagaca tgaacaatcg gacaatcgat ttaactgatt tatcagatac cgggttttta
    13801 ttggtaaata tcgatgcaga agtgttaacg atgaatacgc cacttcaaat cattaatcca
    13861 aatgatcaag tagttgtatt taatgtcatc aacagttctt ctgcattgaa cgtacagtca
    13921 ccaatcaaat acaatgatcg ttcaaatcat gaaacagaag atttttcgga tgcgaatatc
    13981 tcttggaatt ttggaaatga gatgactgat ttgacaatca gtgctccttt tcaaggaaca
    14041 atccttgcgc ctaatgctac tatccgcatt actcaaaacc aagacggaac gattatagga
    14101 acaaatgtga ttttagatgc agctaccaat cgttgggatc ctaacgaaat atttatcagc
    14161 gacaatggta cagatacgac agacacaagc gacagtacag atacatctac cacaagtgat
    14221 agctcagatt catca
//
LOCUS       DAZULY010000054        13917 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000054 DAZULY010000000
VERSION     DAZULY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.055"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1193)
                     /gene="hflX"
                     /locus_tag="ACG81A_001054"
     CDS             complement(<1..1193)
                     /gene="hflX"
                     /locus_tag="ACG81A_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFS5014438.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFA"
     gene            complement(1195..2112)
                     /gene="miaA"
                     /locus_tag="ACG81A_001055"
     CDS             complement(1195..2112)
                     /gene="miaA"
                     /locus_tag="ACG81A_001055"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFS5014439.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2116..2853)
                     /locus_tag="ACG81A_001056"
     CDS             complement(2116..2853)
                     /locus_tag="ACG81A_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS5014440.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2944..3471)
                     /locus_tag="ACG81A_001057"
     CDS             complement(2944..3471)
                     /locus_tag="ACG81A_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014441.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3651..4244
                     /gene="clpP"
                     /locus_tag="ACG81A_001058"
     CDS             3651..4244
                     /gene="clpP"
                     /locus_tag="ACG81A_001058"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFS5014442.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4348..4833)
                     /locus_tag="ACG81A_001059"
     CDS             complement(4348..4833)
                     /locus_tag="ACG81A_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFS5014443.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(4986..5183)
                     /locus_tag="ACG81A_001060"
     CDS             complement(4986..5183)
                     /locus_tag="ACG81A_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014444.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5278..7038)
                     /gene="efrB"
                     /locus_tag="ACG81A_001061"
     CDS             complement(5278..7038)
                     /gene="efrB"
                     /locus_tag="ACG81A_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFS5014445.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7035..8765)
                     /gene="efrA"
                     /locus_tag="ACG81A_001062"
     CDS             complement(7035..8765)
                     /gene="efrA"
                     /locus_tag="ACG81A_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFS5014446.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9158..9370
                     /locus_tag="ACG81A_001063"
     CDS             9158..9370
                     /locus_tag="ACG81A_001063"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFS5014447.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9372..11051
                     /locus_tag="ACG81A_001064"
     CDS             9372..11051
                     /locus_tag="ACG81A_001064"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS5014448.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11305..11505)
                     /locus_tag="ACG81A_001065"
     CDS             complement(11305..11505)
                     /locus_tag="ACG81A_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5014449.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11655..11798)
                     /locus_tag="ACG81A_001066"
                     /pseudo
     CDS             complement(11655..11798)
                     /locus_tag="ACG81A_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            complement(12033..12695)
                     /locus_tag="ACG81A_001067"
     CDS             complement(12033..12695)
                     /locus_tag="ACG81A_001067"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5014450.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            13087..13146
                     /locus_tag="ACG81A_001068"
     CDS             13087..13146
                     /locus_tag="ACG81A_001068"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFS5014451.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            13204..>13917
                     /gene="erm(A)"
                     /locus_tag="ACG81A_001069"
     CDS             13204..>13917
                     /gene="erm(A)"
                     /locus_tag="ACG81A_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFS5014452.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNS"
BASE COUNT         4163 a         2928 c         2369 g         4457 t
ORIGIN      
        1 gcaaaccctt taacttcata ttcgtttgtt tcttccatgt acgcttcgtc caaaaccatt
       61 gtttgtcttc tcaactcgct taatttctgt ccttcgttgc ttggcacaga aagtgtataa
      121 ggggtcatta gttccatcag ctgacgtttg attgcttcga tcaacgccat cttaccttct
      181 ttcgtctgag tagaaatcaa tacatttggg aatagcgtag gcgtgaacat cgctgggtcg
      241 atctggtctg ccttattgta aacagtcagt atcggcattt ctttcatatt cagttcatcc
      301 atcaatttta agacagtctg ctcctgtaaa atacgatctg gagaactagc atcgactaca
      361 tgtagcaaaa gatccatatt ttgactttct tctaatgtag aatggaaagc atcgatcagt
      421 tgcgtaggca ggtcttggat gaacccgacg gtatccgtta cagtaatctc aaatccttca
      481 gcaaaacgcc atctcttagt caatggatct aacgttgcaa acaattgatc tttcgaataa
      541 gtatccgctt gtgtcaatag attcaagata gttgacttcc cggcattcgt gtagccaata
      601 agtccaatct gaaaaatctc gctatttttt cttttttggc gattgcgctc tcgatgagct
      661 tctacttctt tcaattcttt cttaacagca aaaattttat tccgtatatg tcggcggtct
      721 gtttctaact ttgtttcacc tggccctctt gttccgatcc caccgccaag acgagataat
      781 gttttccctt gccctactaa acgaggcagc aggtattcta actgagcaag ttcaacctgc
      841 agcttccctt ctttcgaacg agcacgcatc gcaaaaatat ctaagataag ctgcacgcga
      901 tcaattaccg gaagaccaac agcatcggta atcagttggc tctgtctcgg agttagctca
      961 tgattaaaga tgatcaaatc cgcttcatgc gcatccgctt gttgaactaa ttcttgtaat
     1021 ttccctttac cgataatcgt ttgccgatcc acttgtggtc tcttttgtgt aattgtgaat
     1081 acgacttctc ctgatgcggt attcgtcagc tgaactagct cttccattga ctctgaaaag
     1141 taacgctggt ttgcttcggt ttctacccct acgatgatca ctttttcttc cattttattc
     1201 cgcctccagc cattttttga ttgtttgctc aatctgactt ttttgatttg gctcacttat
     1261 aagatcaaac cactgtgcat ccaaacggtt acgaaaccac gtcaactgac gtttcgcgta
     1321 tcttctcgaa tgtagtttga cttgttctgt tgcttcttcc aatgtttctt tcccttcaaa
     1381 ataagggaaa aattctttat atccaatacc ttgactggcc tggacttctc caagctcata
     1441 caccttacgc gcttctgcta ctaaaccttc tgctagcatc aaatcgactc tttgattgat
     1501 tcgttcataa agaatactac gcttcgtatc taatccaatc atgaggtaat catacaattg
     1561 ctttggctct tctttaggtg ccgtgatact atgtcctgtc gtctctaaaa cttccagagc
     1621 tcttatcact ttgcgttgat tgttccagtg gattttctca gcagctgccg ggtctttttc
     1681 tttcaactga ttccataatg cttgggaacc aattgtttca gctaacgttt catacttttt
     1741 tctaatctca ggattcgctt cttctgtcgc tcctaactga taatcatata gaagggattg
     1801 gatatagaga cccgtaccac cagctatgat aggtaggtgc cctcggttcg caatctcggt
     1861 aatcaattgt cgacctgaat gctgaaaatc agcggctgag taacgttgag taggttcgat
     1921 cacatcaatc aaatgatgcg gaacattatt catctcttct tctttgattt ttgctgtacc
     1981 gatatccaga tttttataga tttgcaatga atcaccactg atgatctctc ctccaaatag
     2041 ttgtgcgagt tccacactta gcgctgtttt accgactgct gtcggaccga cgatcactaa
     2101 tactttttcc atattttacc tacttttcgt tgattgtttc caccgaagag ctttctccgg
     2161 aaaatcagtt attattccat ctattttgag ttcttgacat ttggccattt cttcttcatc
     2221 gttaatcgtc catgcacgaa ttgcttttgg aaaatcagct gcctggactg gatgttcgaa
     2281 caaccaagta ttttttggat gtactccttc tatataaggg gtttctgctg ctagttggat
     2341 ctttttatca gaagtactca tcaataagtc cagctgtatc tccggttcca gtaatgcgat
     2401 tttttccaaa gaatccaaat gaaagctgca atatgcatgt tgaaaaggcc aatctctact
     2461 ttttaatgtt tcggctaatt tttcttcgat accgggatat tcatactgat cagttttaat
     2521 ttcaatcatt agcatccctc ggtaattttt ttggaggagc agtcctaaca cttctctcaa
     2581 ttcaggaact ttagctgctg gaaattcttt gctgaatttg ccccctgcat tcaatttttt
     2641 gagttcttcc aatgtttttt ctcgaatcag ccctttaccg ttagtcgtcc ggtccacttc
     2701 ttcgtcgtgc ataacgatca aataaccatc tttgcttaga tggacatcta gttcgatacc
     2761 atctgcacct actcgtacag cttctgcaaa tgcaggaagc gtattttctg ggtgggtgcc
     2821 gctgcttccg cgatgggcat agatttgtaa catctttctt cactccgttc agttctattg
     2881 tatcattaag aaaaaaagaa agcattacca cgctctcatg tgcaacggca atgcttcaaa
     2941 atatcagctc caaacatcat ctttcgagaa gaaaccaatc ggctggcgcg tcatagtctt
     3001 caactgagct tttttttcat tgatcatttc agtcagacta tccgtaaaat tctctaaaat
     3061 cacgcgtccg cctttataag catatccacc aatcttcatt gtttcatctg gagataaaat
     3121 cacttttcga tgggagatat tcttaaatag ctcgtctaaa atataatttg gcagataaaa
     3181 actattttgc gctgaaaatg attggagata cgcaaaatcg tcaatcgtga tataacaatc
     3241 atcaaatgca tcaaagctga catattcttc catcagcgtt gtccgttttt gcaagatctc
     3301 gttgatcttt ttatttaatt gattcagcgt ctcgatttct ttgactttca attccaattc
     3361 acgtctacgc tgttctttat tttccaagtg acgcatttgc agtatcatgc cgatatagac
     3421 aactacagca cctgtcagca ctccgataag gccagtaata ccaactatca atctgttgtt
     3481 cctccttgtt ttgtttctct gcttatcata ccataaagcg ggtttatgcg actaatttgc
     3541 ctaagttttt tatttgacct tttttgacca aaaatgtata ctacatttta ggtattagaa
     3601 actaaattat ggtacactat ttctaaacga aatcaaggag gaactctatt atgaatttaa
     3661 ttcctacagt cattgaacaa tcttcacgag gtgaaagagc ttacgatatc tactcacgtt
     3721 tattgaaaga ccgtatcatc atgttaagcg gccaagtaac agatgatctt gctaattcga
     3781 tcatcgccca attgcttttc ttagacgctc aagattctga aaaagatatc tatctttata
     3841 tcaattctcc tggtggttca gttacagcag gtatggctat ttacgacaca atgaactttg
     3901 tcaaagcaga tgtccaaaca attgttatgg gtatggctgc ttcaatggga agcttcttgc
     3961 tgacagctgg tacaaaaggt aaacgatttg ctttaccaaa tgctgagatc atgatccatc
     4021 aacctcttgg cggcgctcaa gggcaagcaa ctgaaatcga aattgcggca cgccatattt
     4081 tgcaaacacg cgaacgctta aacaaaattt tagctgaacg cactggacaa ccgcttgaag
     4141 tgattgaaaa agatacagat cgtgacaact atatgacagc tgaacaagca aaagcatatg
     4201 gattgatcga tgaagtcatg gaaaacagtt cttcattaaa ttaatctgtt tcaaaggaag
     4261 cttttttaag ctctcttatc taaaccaaat aaaaaaggat ggtcaactca aactgaccat
     4321 ccttttttat tttatggatg ctactgccta tctttgctcc ttgaattttc cttgcgcatg
     4381 catccgacct atctcgacat aatgctggat gttttctttg tttttttgta tttcttcttt
     4441 tgtcaaagga cggatcactt tagctgggcg accaaaagcc aagacattag gaggaataac
     4501 ttttccttct gttactaatg atccagcacc aatcaaacta ttctcaccaa taaccgcatg
     4561 attcaatatc gtggtcccca tcccgatcag cgcccctttt tcgattgtac agccatgtag
     4621 catgcactga tgtcctaccg tcacattctc cgcaatatct acaggtgcat catgatcgac
     4681 atggatgatt gtaccatctt ggatattcgt tctttgtcct atttttatcc aattactgtc
     4741 cccgcgtaaa acagcttgat accagatcgt cacatcttct gacaatgtca catcaccaat
     4801 cactgtggca tttgatgcta taaaccgttc catatttatt tttctccttt cattttttgt
     4861 agaaactaat cctattgaat aaaggtatca tagcatatat ttatttcata agaaaaaaaa
     4921 caggtaaaac ctgctcaggc cagatgtcta tttacagtat ctggtaactg acgttttacc
     4981 tgtttttatt gtacatctat attttcttct attacttttt ttaaaattaa ttcgtacact
     5041 tctatttgtg ctgccaagcg ttcgatttga aactcatttt caaaaaaatc agctgaatca
     5101 aaaggcttcg tctgacagac gtgcgttaaa cgatcaactt ctcttttggc ttcagtaatt
     5161 ttttcattta aaaactgttc catctccgtg tcctcacttt tcttgataac tttactttac
     5221 cattatcaag aatagagaac aaaaaataag ttttaaactg ttttattctt atatgactta
     5281 ttctgcttcc tcagcaaatt ggctgttata cagtttttca taaaatccac cttgtgccaa
     5341 taaactctca tgcgttcctt tttcaatgat ctgaccttga tccatcacta gaatcagatc
     5401 tgcatcacgg attgtcgata aacggtgagc gatgacgaaa cttgtccgtc cctccatcac
     5461 gcgatccatt gctttttgaa tcaacgcttc taaacgggtg tctactgaac ttgttgcttc
     5521 atctaaaatc aagattttag gatcggagac cactgctcgg gcaatcgtca gcaactgttt
     5581 ttgtcctaaa gacacattgt ctccctcaga attgatggcc atatcatatc catctggcat
     5641 cgttcggata aaatgatcta cgtttgctgt tttcgcagca tcaactactt cataatctgt
     5701 cgcatcaagc tttccgaagc ggatattatc tgcaatcgtt ccttgataaa gccatgcatc
     5761 ttgcaagacc ataccaaata gcgaacgcac atcactgcga ctcattgatt tggtgtcgat
     5821 tccatcaatc cttattgctc ctttgtctac gtcataaaaa cgcatcaata gattgatcaa
     5881 tgtcgtcttg ccggcaccgg tcggaccgac cacagctacg gtttgaccag ctttcacttc
     5941 aaaattcaaa tcttggatta atggtttctt cggatcatat gcaaaatcga catgatcgaa
     6001 tgtcacatct cctaaaatcg tttctggcag tgaagcatct actttgtttt ctttttcttc
     6061 tggctcatct aaaatctcga atacacgttt cgttgccgct gatgcacttt gcaaaactga
     6121 agacaattgt gtcacgttcc ccattggctg actgatttgc caaatatatt ggatgaatgc
     6181 ttgtagttga ccgacaacga tcgccccagt aataacataa aagcttccta gaacagccat
     6241 accaatataa gtagcatagg cagttaattg aactagaggc atcatcaagc cagaaatgaa
     6301 tgctgcacga aacccaaagt atttcaagct gtgattaact tttttgaatc cttccaatgt
     6361 ttctttttcg cgtccatata ctttcaatac actaaaacct gtcatatttt cttggacgta
     6421 gccgtttagc tcaccaagag aattctgcat gctttgaaaa tatttctgcg aagcattgat
     6481 cactcgtttt gagatccaga tcgatgttgg aatcatgatc atagagatca gcgccatcat
     6541 cggctggata taaaacatca ttgctgcagc caacgtaatt cccaatatcg catttacgat
     6601 accaataaaa gcttgttgta aagcaccgct gactgcatct acatcattcg tcacacgaga
     6661 caaaatattt ccttgctggt ttttatcgaa atatgaaacc ggaagacgat tgattttttc
     6721 ttctatgtct cttctcaaat catgcatggc actttgaacg acatttgtca tcaaaacacc
     6781 agaaagtaat tgcgaaatac aataccctat tccaacaact gtgatccaaa tgagacattt
     6841 gaatatataa gaaaaattga ttgattcgcc attggcaata ttacggctga tttctgtagt
     6901 aggaagtcct gtaacatatg gcaaagccac gttgaaagcc actgtcagaa ctgtaaatag
     6961 gatcactaaa agaaaagtct cacgatacgg tctgatatat cgagctaaac gttttaagga
     7021 agaaaaggtt tttttcatgc taattcctcc tttgataatt gagaagcggc aatatcataa
     7081 taaatcgggc atttttccaa caactcccga tgcgtcccca taccgacgac ttctccttca
     7141 tttaatacga tgatcttatc tgcatgcatg atcgttccca cacgttgtgc tacgatcaat
     7201 acagtagatt ctgtcgtttc ttttttcaga cgagcacgta acttcgcatc cgtttgataa
     7261 tctaatgcag aaaagctgtc atcaaaaata tagatttccg gacggcgtat gatggctctg
     7321 gctattgcca atcgttgttt ttgtccacct gaaaaattgg aaccaccttc tgataaatgt
     7381 tcattaaatc catctggttt tcgagaaatg aattctgaag cttgagcaat ctcagctgca
     7441 cgttccattt cttcttgcgt agcatcttct ttcccgtatc gcaaattatc tgcaatcgtt
     7501 ccagtaaata gcagcgcttt ttgcggaata aacccaatct tatctcgcaa cttgcttagc
     7561 cgataatctc tcacgtctac tccatcgatc aagatctttc cttcactcac atcataaaag
     7621 cgagggatca attggatcaa cgtagatttt ccgctaccgg tacttccaat aaaagcaacg
     7681 gtctctcctg gagaggctgt aaaactgaca tttcgtatca ctggctcttg cgaatggcct
     7741 ggataagcaa atgttacatt ttgaaattcg atataccctt tagtttttgt atcagatata
     7801 ccatttggat cttctacaat cgaaggtttg gcatctaggg cttcttggat acgtccagct
     7861 gaaacagctg ctcttggata catcatgaac acactagcaa ataacatgaa tgagaacaag
     7921 gcatggaata tatactcgat aaacgcaatc aaatctccta cacgaagctc gcctgcatcg
     7981 atttgcactg ccccgctcca gatgatcaat accattacga tattaaataa gaaataaaaa
     8041 cccggttgtg caacagccat caaacggaat aagctttttg aactagaagc ataattctca
     8101 ttgactacag caaaacgttt ttcttcaaac ttttcattga cgaatgcacg aatcacacgc
     8161 aatcctgaaa gattttcccg caagatactg ttgattttat ctaagttttt ttgttgcttt
     8221 ttagataacg gttcagacac cttagcaatc gctacgactg ccaataatag taaaggtaat
     8281 gcacctacta catacaaact gagtgtcaca cttgtacgca tgaccatgta caaactgaca
     8341 atgaacatca ttggtgccat aaaaccgata cgtaaaatat tttgcaggaa caacatgatc
     8401 tgataagcat cgtttgttgt acgagtgatc aatgaagata cgcctagatt ttcgtattcc
     8461 tgatgcgaat aactttggat cttttcgaac aaatcatctc gaatatctgc tacgatatta
     8521 gtcgtaatcc tcgaaccaaa ataacctaac aagatattca aaataatacc gattacagta
     8581 ataacgatca taaatatccc ttgttgcttc acataatcaa aatcatcatt gcggattcct
     8641 acatcgatca tacgtgctaa aatcgtagga agtcctaatt caatcaaaat aaaaccaaat
     8701 acgcatatga aattgaaaaa caagaatttc ttataacgca tggtataatg ccacattagt
     8761 ttcactttct catctccttt taatcttttg ttacattttc attactaaaa atgatagcac
     8821 aacgagccta ttaatagaac acttctctca taaaaaagtc attatgatta tagcgggatg
     8881 ataaacattg attttttctt tcaatctagt tgatttctat acttatttgc tgttttatgc
     8941 tagaataaca attaattgtt gtatattttt tatacagaag gtacggttga aaaaatatcc
     9001 gaacagataa gcaaaaacga ggcttgtctc tttgactcga atccagtatt ctagtcaaag
     9061 catatgaaaa accaggtggc ttgtcccctc gttttgtgtt gtctaaaaaa atcaactagc
     9121 taaatcactg ctttacgcag ataaaggaga caaaacaatg atctataaag tttattatca
     9181 agaaacaaaa gtacgcaacc ctaaacggga agaaacaaaa tctctttaca ttgaggcaaa
     9241 aagcgatgta gacgctcgcc aacgtgtgga agaaaatacc ccatacaata ttgaattcgt
     9301 tcaacttcta gaaggaagtc atctagagta cgaaaaagaa aatgctgatt tctcattagt
     9361 ggagttttaa tatgaaactt tccatcaaaa ataacgagac aggcgtcttt gctattggtg
     9421 gattaggtga aatcgggaaa aatacgtatg gggtacaatt ccaagatgaa atcattttga
     9481 ttgatgccgg gatcaaattc ccggaagacg atttacttgg aattgactat gtcattcccg
     9541 actacagcta catcatacaa aacttacaca aagtaaaagc cttggtgatt actcacgggc
     9601 acgaagacca catcggcgga ataccctact tgctgaaaca agcaaatatt cctatctatg
     9661 ctggtcctct cgcattagcg ttgatcacaa acaaattaga tgagcatggc ttgcttcgag
     9721 atgcagagct ccatgagatc aatgaagata cgatcatccg ttttagaaag acttctgtca
     9781 gctttttcag aacgacccat agtatcccag atgcattagg gatcgtggtg aaaacacctt
     9841 ctggaaatat cgtagcgact ggagacttca aattcgattt cactcctgtt ggcgaaccag
     9901 caaacttgca taaaatggct cgtatcgggg aagaaggagt cttgtgcctt ttatctgata
     9961 gtacaaatgc tgagattcca acatttacta aatcagaaaa aacgattggg cattctattt
    10021 tgaagatctt ttcaaaaatc gaaggacgta tcatttttgc cagctttgct tctaacatct
    10081 tccgtttgca gcaagcagca gatgccgctg tggcaactgg tcgtaaaatc gctgttttcg
    10141 gacgaagcat ggaaaatgcc atcgtcaacg gtcaacgttt aggatatatc aaagttccag
    10201 acggcacatt tgtagatgca cacgaaatca atcgcttgcc tgccaatgaa acgatgatcc
    10261 tatgtacagg ttctcaagga gaagcgatgg ccgcgttgag ccgcatcgcg aatggtactc
    10321 accgtcaaat ccaaatccag ccagacgata cagtgatctt ttcaagttca cctattcctg
    10381 gtaatacaac aagtgtcaat catttgatca atttgctgtc tgaagctggt gcagaagtga
    10441 ttcacggaaa aatcaataac atccatactt ccggtcacgg tggacaagaa gaacaaaaat
    10501 taatgcttcg tttgatgaag ccaaaatatt ttattcctgt ccacggagaa ttccgcatgt
    10561 tgaaagtaca tgcttctcta gcacaagatg taggtgttcc tgcagaaaac tgcttcatca
    10621 tggaaaatgg cgatgtttta gcgttaacta gcgattctgc gcgtcctgct gggcattttg
    10681 tggctggaga tgtgtatatc gatggtagcg ggatcggtga tattggtaat gtcgttttac
    10741 gtgaccgtca cctgctttca gaagaaggtc ttgtattagc tgtcgcgaca gtggatatca
    10801 agaaaaaaga aatcttggct ggtccagata ttctctctcg tggatttgtc tacatgcgag
    10861 aatcaggaga aatgatcaat gaagctcagc gaatcttatt ccacgcttta agagaatgtt
    10921 tgaatggacc tgactgttca gaatataaat tgaaagaagc gatgactggc gcgcttcagc
    10981 ctttcttatt cgaacgtaca gaaagacatc caatgatttt gccaatggta atgacaccag
    11041 atgaagaata aataagaggt tatgaaatca gcaccttgtc ctgaatataa gagaaaacaa
    11101 atgaaaaaca tgagaagcca tttttgtttg ttcttattcc ttgatacaag gtggctcttt
    11161 catagcctct taaatttatt tactttttca tacaaaaaaa tacctgccac aagagaaatt
    11221 gtctcttgtg gcaggtattt ttgttactcg ctttgttctc tcgtaaaaat tgatgtctac
    11281 catcaatttg atttacagat aaaattaagc ttttacaacg tttgcagctt gtgggccgcg
    11341 gtctgattct tcaacgtcga aagtcactgc ttgaccttct tctaatgttt tgaatccgtc
    11401 accttggata gctgagaagt gaacgaatac gtcgcttcca tcttcacgtg agataaatcc
    11461 aaatcctttt tcagcgttga accattttac tgttccgttt tccataataa tatcctcctc
    11521 gtgccaaaag cactaattta aattgtaaca ttgctcgtgt acgatagagg tgcaacagat
    11581 gtttcaactt ctattcccaa acaaaattac aatataattg tacacttgct tcattttttt
    11641 tgcaagaaag caacttactt ctcttccatc attccttctt ctctcaaact aaaatatcgt
    11701 ttgcgtccga tgatcaagtg gtccagtaca gtgatcccca tcatttctcc acacttttgt
    11761 atccttttgg taaaattcat atcctgctgc gatggaattt catattatca agagtaaatt
    11821 acaaaagaat catttaaatc aatactttca ctttgaatac atgtatttga agtggaaggt
    11881 acttatttca aaaatagtaa aacctgtatc ttaaattact taatagtaac ataagataca
    11941 ggctgatttt ttattcattg ttatttatac taaagcaccc gatagctctg aaaacaatca
    12001 caaattcaac ttttcaaagc cacagcttta agttattttg tccagacaac ccccattgcc
    12061 caacccattt tatggaattg gcatccaggc aacaactttt catataaatc gtaataattt
    12121 tgttcagata ggtacttatc tgaagctaaa tgctcaagcc atgatttaga tgtgttgtga
    12181 ttataaattc taatcgcatt ttttattcca aatttgaaac aatgcggtaa aaactcttgg
    12241 attgccccta atttataaac ataggtaggc ggtgtctcga cttcagatac attatcaaga
    12301 ataactattc ttccttcctc attcaacagt tccttcattt gctgtattac gctggctata
    12361 tcatccaaat gatgaaaggt tgttcggctt acaataaaat caaacttctc attaaaatta
    12421 agttgttctg cattcatatt cagatagacc gtatttgtta gttgacgttt agatttggca
    12481 agatcgagca tttgattaga aatatcaatc cctaccactt catcataata acttgctaat
    12541 ttctccacta acaaacccga gccacatccg atatctaatg ctctgccttt ctttggagac
    12601 atattagaca caaagaatga ataatcattc aaaagctcat tcacgaaatc gtaatcttct
    12661 gcaaccttat caaactgtga ttctattgta ttcaaaaaga tcccccattc ctactttatc
    12721 gacattcttt cattacttac cactttagat gttttttcgt tggggataaa acttcccttt
    12781 agacaatttt atccaaagac aatacaacag tgcaacttta ttaaagtcac tgtcctttat
    12841 cgcagccttt actttttagt aaagacagtg gcttctctta tcaagtttca aaacatatta
    12901 ttttgaagaa aacgtccatc tgaagtgtca agtgcaaaat tacatataaa ggtttattct
    12961 aaaatgaaaa gatgatacaa tcatattcag ttacataagg aggtttcaat tatgtgcacc
    13021 agtatcgcag tagtagaaat tactttatct cattcataat gaaaaaaatg gaaaggagat
    13081 aaaagtatgg gtactttttc tatatttgtt attaataaag ttcgttatca accaaatcaa
    13141 aattaattgg ttataatgaa cgcttaatgt cagttcatta taaccagtaa ggagaaggtt
    13201 ataatgaacc agaaaaaccc taaagacacg caaaatttta ttacttctaa aaagcatgta
    13261 aaagaaatat tgaatcacac gaatatcagt aaacaagaca acgtaataga aatcggatca
    13321 ggaaaaggac attttaccaa agagctagtc aaaatgagtc gatcagttac tgctatagaa
    13381 attgatggag gcttatgtca agtgactaaa gaagcggtaa acccctctga gaatataaaa
    13441 gtgattcaaa cggatattct aaaattttcc ttcccaaaac atataaacta taagatatat
    13501 ggtaatattc cttataacat cagtacggat attgtcaaaa gaattacctt tgaaagtcag
    13561 gctaaatata gctatcttat cgttgagaag ggatttgcga aaagattgca aaatctgcaa
    13621 cgagctttgg gtttactatt aatggtggag atggatataa aaatgctcaa aaaagtacca
    13681 ccactatatt ttcatcctaa gccaagtgta gactctgtat tgattgttct tgaacgacat
    13741 caaccattga tttcaaagaa ggactacaaa aagtatcgat cttttgttta taagtgggta
    13801 aaccgtgaat atcgtgttct tttcactaaa aaccaattcc gacaggcttt gaagcatgca
    13861 aatgtcacta atattaataa actatcgaag gaacaatttc tttctatttt caatagt
//
LOCUS       DAZULY010000055        13898 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000055 DAZULY010000000
VERSION     DAZULY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.056"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..1545
                     /locus_tag="ACG81A_001070"
     CDS             121..1545
                     /locus_tag="ACG81A_001070"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5014453.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            1844..2551
                     /locus_tag="ACG81A_001071"
     CDS             1844..2551
                     /locus_tag="ACG81A_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS5014454.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            2538..2861
                     /locus_tag="ACG81A_001072"
     CDS             2538..2861
                     /locus_tag="ACG81A_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS5014455.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(2950..3987)
                     /locus_tag="ACG81A_001073"
     CDS             complement(2950..3987)
                     /locus_tag="ACG81A_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS5014456.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(4308..5291)
                     /locus_tag="ACG81A_001074"
     CDS             complement(4308..5291)
                     /locus_tag="ACG81A_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFS5014457.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(5706..6476)
                     /locus_tag="ACG81A_001075"
     CDS             complement(5706..6476)
                     /locus_tag="ACG81A_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5014458.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6469..7404)
                     /locus_tag="ACG81A_001076"
     CDS             complement(6469..7404)
                     /locus_tag="ACG81A_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5014459.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(7720..9369)
                     /locus_tag="ACG81A_001077"
     CDS             complement(7720..9369)
                     /locus_tag="ACG81A_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS5014460.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(9486..12089)
                     /locus_tag="ACG81A_001078"
     CDS             complement(9486..12089)
                     /locus_tag="ACG81A_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFS5014461.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12101..13150)
                     /locus_tag="ACG81A_001079"
     CDS             complement(12101..13150)
                     /locus_tag="ACG81A_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS5014462.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13176..13490)
                     /locus_tag="ACG81A_001080"
     CDS             complement(13176..13490)
                     /locus_tag="ACG81A_001080"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFS5014463.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(13506..>13898)
                     /locus_tag="ACG81A_001081"
     CDS             complement(13506..>13898)
                     /locus_tag="ACG81A_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5014464.1"
                     /translation="DKKSVVEKLSKLLETNGFVSDHKQFQKDVFDRENHMTTGIGKNI
                     AIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLLAIKDTDKGTNHLRILAEL
                     SGRLMDDEFVKRIKDSRNKKELVKALSF"
BASE COUNT         4104 a         2888 c         2378 g         4528 t
ORIGIN      
        1 cgataaaatc cacatcaaaa ttatacacac aataaaaaag atatatatat ttaaacaaaa
       61 ctatttactt taagttgtaa gcggtgttat cattaagtgg aaacaggagg taaaaaaatt
      121 atgacaacta tgaaaaagct aacttttcca gatgattttt ggtggggttc cgcttggtca
      181 gctgagcaag cagaaggaaa aggagaaaca ggaaaagcag agactatctg ggatcgctgg
      241 tttaaagaac aaccttaccg gttttatcaa cgaatcgatt cctctattac aaccgatcac
      301 atccatcaat ataaagaaga tatagaacta atgaaaaaaa ctgggcataa ctcctttcgc
      361 gtttctattt cttgggcacg aatgtttcca aatggcggta ccggaaaagt aaatcccaaa
      421 gccatttctt tctatcgaaa attattttcc gaaatgaata ataacggaat caaagtattt
      481 gccaacttat atcattttga catgcctgct actcttcaag ataaaggagg atgggaatca
      541 cgtgaagtag ttgatgctta tgttcattac gctgaaactt gttttaaaga atttggtgac
      601 ttagtttgtc attggtttac cttcaatgaa ccacttgggc ctattttagg tacttatctt
      661 gaagattttc attatccaaa cctcattgac tttaaacgcg gagcgcaagc agcattcttc
      721 actatacttg cacacgcaaa agcaatacaa gtatttaaag aactagctct tcctagtaaa
      781 ataggcgtta ttctaaattt gagtcctact tttcctcgta gtcaaaatcc tgctgatgtg
      841 aaagcagcag aaatcgctga tttattttat acgcggagct ttttagatcc tatggttaaa
      901 gggaattttt ctaaaaagct tatcctgtta ttacaagaat ataatcagct tcctgaagat
      961 tatacgaaag aagatcttgt atgtatctac gaaaataccg ctcaaattct tggactaaac
     1021 tattacgagc cacgtcgcgt caaatctcgt tcttctatgg tggatacatc tagtccattt
     1081 ttaccagagt ggtttttcga accacatatc atgcctggac gacgaatgaa cgaataccgg
     1141 ggatgggaaa tttacgaaaa aggaatttat gatttgtgca tggatatcaa gaaccattat
     1201 ggaaacatcg aagcatttat ctcggagaac ggaatgggcg ttgcggatga agaacgtttc
     1261 atggatgata cagggcaaat aattgatgat tatcggattg agtatatcaa agatcatctt
     1321 gcttacttat ggaaggcaat aaatgaagga tgtaacatta aggggtatca tctttggaca
     1381 tttattgatt gctggtcatg gatcaacgcc tataaaaacc gatatgggct ggtttcactc
     1441 gaccttacta ctcaaaaaag aacaattaaa aaaagtggcg aattttacaa gcaattaagt
     1501 gatacaaatg gatttagata cgatgtagac ttgttagttg aataatattc taaaattcca
     1561 cttgaagtga acacattaga aaacaagaat aatgagaaat accaataaca aatacttttt
     1621 cttcatactt catttatact tgtcagtata agagactgtg acaaatgtag tgatcctcag
     1681 aagttaggtc tggaaatcta acttgagaag atttttttaa actacgtcca gtctttttta
     1741 tttgtttaaa attgaagttg acaagtcttc attaaaaatt tagaatattc acaagtttga
     1801 cgacaattat gaaaatttat gtcgtgctaa aggagttttg ttattggatg aaaagttaga
     1861 tataaaaact gcgatcaaag acacgttacc taccgttttt ggttatatcg gtattggact
     1921 tgcatttggt atcgttggga aagctgccgg gtttcatccg ctggtcgtca cgttgatgtc
     1981 cctgctgatc tatgctggtt ctgcccaatt tatcacggtc agcatgcttg ctagccatag
     2041 tccactgctc tccatcgttc tctcgacctt tctagtcaat tcccgaatga ttctgatgag
     2101 tatgacgatt gctccttatt tcaagaaaaa tcggctgctt caaaatctgc tgattggcac
     2161 attgctgaca gatgaaagtt tcgcactggg aatgaataaa ttgaactata ctggacaaaa
     2221 gttaaacttt cgttggatga atacagctaa ttggatttct tatttgacgt gggtgggttc
     2281 ttctcttgtt ggcgcacttt tagggaattt tattactgat ccgaaaaagt ttggtttgga
     2341 tttcgccatc gtcgctatgt ttatcgggtt actttatctt caagtcattt ctgatagaaa
     2401 cacctctaaa cgattacagt tgatcctgat tggtctcact ttgattctgg tatatgtcgg
     2461 actgattttt atcccaagta acttagtgat tttagttgta acactgattg ggtgcggatt
     2521 gggggtgtgg atcaaacatg ccttcttttg agtttacatt gctgactatt ttaggttgta
     2581 ctttagcaac ttggctttct aggatcctac catttgtctt actgaaaaaa ttcgatttgc
     2641 cgcaaccgtt gcttgagtat ttaagttttg tcccgatcgt cattatgtct gctttatggt
     2701 tcagcagtct atttacccaa aatatcggac atctgccgca gatcaatctc gaaaatgctt
     2761 tggcttcagt tcctacactt ttagctgcta ttctttcaaa aagcttattg gtgatcgtct
     2821 tggtaggaat tctttccctt tcgttgattc gcttattata aataaaaaaa caagagctta
     2881 tggcaaatta ctgtcatgcg ctcttgtttt tatcaaagtc aaataagaaa aaaatcggcg
     2941 tattcttgct tatccgttga acggaccact aacaacgact tttcctaata tatgtcctgt
     3001 ttctacgtct ttggttgctt gtttcaagtt cttcgcattc atcccatctg aatagacctt
     3061 gctcaatgta ctgcggataa tcccttcatt tgctaaagca gcaatgtgcg ctaaagcttc
     3121 tccttgcgtt tcgatttcat agttatagtc tgttttagcg aacatatact cccagtcgaa
     3181 gctgatagat tggtttttcc aaacacctat atctaacggc tctttgatcc ctacgatcgt
     3241 tcccacatgt ccaaatggtc ggacaaggtg tttgatttga tcaaagtagc gggtaatatc
     3301 gaataataca gcgacatact ctacttgatc gattcctaat tcacgaaaac ttttacttaa
     3361 gtcgtgatga taatcgattg gatagtctac acctgttttt ttcagccatt caaaattctt
     3421 cggacttgca gtcgcgtaga cttctaggcc agcccaatgc gctagttgat tcaaaatcga
     3481 accgacgccc ccagctccgt tgatcactaa gatttttttc ccgcggttgg cattttcttc
     3541 tggagtcaga ccaaattttt caaataacaa ttcataagcg gtcaatgagg tcaacggcaa
     3601 ggctgcggct tcttcatctg aaagattttt tggtgcaaga gcgacaatac gttcgtcaac
     3661 cagttggtat tcttgattgc ttcctgctct tgtcgttgta cctgcgtaaa aaacgcgatc
     3721 gccgacagaa aatttttgca cttcatcacc tacagcaaca actttcccga ctccatcaaa
     3781 ccctaagata cgtaaagcat ttcttatccc gttatcttgg cgaagtttcg tatcgacagg
     3841 attaacagat actgcgttga cttttaccag aagatcccgt gaactgggta caggtatctg
     3901 ttctttttca tcaataaagc tttttggatc atcgatcgac aacccttcat aaaaaccgac
     3961 taccttcatt aattcctttt ctgacatacg aattcctcct tagcttcatc aagttggagc
     4021 atccatacag ttttcatttc aatatatata gtatgctatt tgttccactt ttattcatca
     4081 taattattct cttacttaaa gtaaactatt tgcacttttt tggttaaaaa agaggttgcg
     4141 acaagacttt tgtcacaacc tcttttaaac ggtgttcaat caactgaccc tcggtattcg
     4201 ctcaaaaaac gacaaaatat gaggagctat ttttgtttgg ctgttcttat tacttgatgc
     4261 aggacagctt tttcacgacc tctaattcac ggtgttcaag caagaaatca ttgttcactg
     4321 aaaagcgttg gatgttcttt ttcaaaggtg tctaagatct ctcgtacttc atcagtagat
     4381 ttcgtattca tcaactgaac tcgcaagtca tttgctcctg gaaatccttt gatatagatt
     4441 ttaaagaaac gatgtagtcc tgtgatggaa cgcgggatga tttctgaata atgatcttgc
     4501 agatctagtt gcagacgaag tagtccgatc agttcttttt ctgtatgttc cctcggttct
     4561 ttttcaaatg cataaggatt cttgaaaatc ccccggccaa tcatgatgcc atctacgcca
     4621 tatttttcgg ccaattcttc tcctgtctgg cgatctggga tatctccgtt gatcgtgatc
     4681 agtgtttgcg gagcgatttg gtcacgtaaa gcaatgattt tagggatgac ttcccaatgt
     4741 gcatctactt tactcatctc tttacgcgta cgcaaatgga cagaaagatt ggctatatct
     4801 tgatgaagta ggtgactgat ccattcttcc atctcagaca tttccgtata gccgatacgc
     4861 gtcttgacgc tgacaggcaa accgccagct ttagccgctt caatcaattg tgccgctact
     4921 tctgggcgaa ggatcaagcc actgcctttt ccacgttctg ctacattagg gactgggcag
     4981 cccatgttga tatcgattcc ttggaagccc atttctgcca tagcaatgct catttctcta
     5041 aaaaattctg gcttgtctcc ccaaatatgg gcaaccatcg gctgttcatc ttctgtaaag
     5101 gtcaatcttc ctcgtacact gtcttttcca tctggatgac agaaactgtc tgagtttgta
     5161 aactcagtaa agaagacatc cggtgctgct gcatgcttta cgacatggcg aaatacaaca
     5221 tctgttacat cttccattgg tgctaaaaca aaaaaaggct ttggcagctc tttccaaaaa
     5281 ttagtagtca tttatttgtt actctccatt ctatcaagca gtgaagtcgc aatctttctc
     5341 aaaaacattg tcattatact aggaaacaaa aaaagaagca aagtgaacag cttaaaagat
     5401 gtttctgatt gagcaaaaaa agactgtgac aaaaatctta tcacaattcc ttttccggaa
     5461 taaatggtgt tcaaaagctt gcatctgcgg taataagtcc aaccaaacga aaaataggag
     5521 gagctatttt tgtttggctg ttcttattac ttgatgcaga acagcttttt cacaacctct
     5581 ccactaaacg gtgttcaatc agctgaccct cggtattagc tcaacaaacg acaaaatatg
     5641 aggaactatt ttcccgtttg tttctctaat actcaggtca aaacgctgat ttcacaacct
     5701 cttttctaac ttctcaatcc tcttccttta ttgatcaaat accaagtaac ggcgtacaaa
     5761 acaacgatga atagcacaag tacgatcatc gatgttgcta ctggcacatc gattgttcca
     5821 aggaatccgt aacggaaacc ggagatcata taaacgatcg gattgacttt cgaaacagct
     5881 tgccaaaatg gcggcaacat agagatggca tagaaaacgc cacctagata agttaacggc
     5941 tgcaagacaa atgtaggcac gatcgataca tcgtcataag actgagcaaa aatcccgttg
     6001 agcagtcctg ctaacgaaaa caagattgcc gtcatcaata atgtaatgat gaccatgaac
     6061 catgaataca catgaagtgg tacaaagaac aaggaaatga tcgttacgag agagccgacg
     6121 agaatactgc gacccactcc gccaatcaca aagccccaaa tgatcacatg cgtaggaaca
     6181 ggcgctacca agatttcttc gatgtttttc tggaattttt gagagaagaa ggacgaagaa
     6241 acgttggcat acgaactagt gatagcagac atcataatca aaccaggcac gatgaattcc
     6301 atatatgaaa aaccacccat gtcaccgatc cgaccaccga tcatttttcc gaaaataaca
     6361 aagtacaagg atgtcgtgat cacaggcgga accagtgttt ggacccagat acgcaaatag
     6421 cggttcgttt ccttgatagc taaacttttt aatgcagtaa aataaagatt aaacattttt
     6481 ctcctccgta tgattatttt cttctgtgat tttcaaaaac agttcttcta aacgattcga
     6541 cttattcctc atagaaagaa ctttgattcc ttgtcttgtc agctgatcga agattccgtt
     6601 caccccttga tttcgttcaa cttcgacagc gattgtctgt tcatcttcaa atgtacaagc
     6661 gtatccttca atcacaggtt tggtctcata tggcgctaaa tcaaagataa atgtttcaaa
     6721 ttgcagtttg gatagaagct ctttcatact agtattctcg atcagttcac ctgattggat
     6781 gatcccgatg ttgcgacaaa gcatttcagc ttcttctaaa taatgcgtcg tcaaaatgat
     6841 cgttgtacca ttttcgttca attctcggag aaactcccac atctcgcgtc tcaattcaat
     6901 atcgactcct gctgttggtt catccaagat cagcaaacgt ggttcatgca tcaatgccct
     6961 agcaatcatc agtcttcgct tcattccccc ggaaagcata cgtgcacgga catttctctt
     7021 ttcccaaaga ttggactgct tcaaatattt ttcgctgcgt ttcatcgctt ctttgcgaga
     7081 gacaccgtag tagcctgctt gattcacgac gatttgctgg accgtttcaa agggattgaa
     7141 attgaactct tgcgggacta atccaatctg ttgctttgct cggactagat ccgtatccag
     7201 atcataaccg aagacattca cttttccaga tgttttattg actaaagaag taatgattcc
     7261 gatcgttgta gacttcccag ctccatttgg tcctaatagc gcatagaaat ctccttcttt
     7321 cacggaaaga tcgattcctc gcaatgcttc gaccccactt ccataaacct ttttcaaatt
     7381 atttatttca agcgcatttg ccatttattt cctatctcct tatagacatg attctttatt
     7441 gtatcatgga atacagcaga tgaaaaaaca acttggttcc agcgaaaaat caggttgctg
     7501 ggctcaggaa aatctcctaa tgaaagacat caatcggacg aagtcagaca accttttcag
     7561 aaaaaacaaa tcgaagataa aggattacta ttcatatgtg caacaaaagg agcgtgagac
     7621 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg aagcaaaagc
     7681 caagattttt gatccaataa atactttcga tcacttcgcc tatcccaata catgccactg
     7741 ccgcttttca tcaaataacg cggcttccgt ttttgcggtt tctactaaag tctctgggta
     7801 atccaaaact ttcagcaagg caatcgcatt tcgggtcctt gaaggtcctt cttttaattt
     7861 gaaatcaaaa ctgattcctt ttccttctgt tacttgttct tcaaagtgga cattctcaca
     7921 ataattcttt aaaatctctg tcaattcgat atcatgtgtg gcgacaaatg ctaaagatgg
     7981 atactccgca agccaccgaa cgactgaagc ggaagaagca atccgttcga tcgtatttgt
     8041 cccttttaaa atctcatcta cgaagcagta gcagcgttct ttggaagcca cttggtccaa
     8101 caaacgtttg atcgatttga tttctgaaac aaaataactg tcgccttcaa ataaatcatc
     8161 ttcgattgcc atggaagtca acacatgtcc aggttgcagg gtaaactgtt ctgcaatcgc
     8221 tgtttggatc gtctgtgcta gaatacagct gatggctata cttttgacat acgtagattt
     8281 tcctgatgca ttggaacccg tgactaatgt attcttctgc cagttgaccg gattcactac
     8341 cgcttctttt aacaatggat gatagatatc tttcgctgtc acaccgccct cagtaaactc
     8401 tggtcggcaa gtgatgggca tataagtacg gtaattcaat attgcagcgg ctacttctaa
     8461 ttctcccagc aattcccaca gcttgatggc ttcagttgaa tgcttttcta atttagtgag
     8521 aacgaaatga taggagataa atggcagcat gaacatgatg ttgacatatt caaaaatcaa
     8581 ctctgcttca ctcccactct tcatacgaaa tgaaaaacct aatttaggga tgtctcttaa
     8641 tggtttagtt tgctgtttca gctcttcttg aagtggtgtc ggcagttttg ctagctgatt
     8701 ggcggctgaa atcgttgaaa ccaaataact catgctattc agctctgttt ctagtgtttg
     8761 tttcttcact agataataga ccgtattgaa tactaaccca ccgattgcca aatagattcc
     8821 tagtacttgt cctaaaagaa gcaaacccaa acccaccagc ggaagcaagc caagaaaaat
     8881 aaataaacct acgcgtccaa tctttttact ttttccatcc gctagatatt gttttgcaaa
     8941 attatgatct ttttttccta aacgagcgaa ttgatattgg attttttctc tcagttgagg
     9001 attttcctga taaaaagcaa tcagtttttc taactgctga tcttcaccaa aatcaaagct
     9061 gcgtaaacgt tgatacaacg cttctgaacc tacactagaa taggtactgt tgattccttc
     9121 gaatacttcg aacatatcca ggtcatacca agtaagatca tcgatttcgc tatcccatga
     9181 acggaacttc ttctccgtga gccatgcttc cttcaagctt tcttctttat caaatctcgg
     9241 ttgataagga attttttccc atttgctacg aactgtttcc tttaatttca aacgtgaaaa
     9301 aatgtctaaa gcaactaata aaataacagc tgcaataata attaaaacga taaattctct
     9361 ttgacccata tccctctccg ttcctttatt tgttagcttt agtatagcac tgttgcgcta
     9421 tttgaaaaga gggtaaaaaa acacctttca aaataaaagg tgtttttttc ttgtatatta
     9481 catatttatc tctcatttat caaaattgac aaataatcat agggaggaat tgttgttaat
     9541 tcttgtttat tctccagtgc attgacaact gtcccaaggg aaaggatagc ctcaggtact
     9601 attttttctt gttctgttgg attagctaag cgaagaacaa atccttgttc atgaaaataa
     9661 gaaggataaa tcgatgatac aattaagtct tcaggtaatt caatcaatga aaatttacga
     9721 ggaacatcaa gttcattttg taatggccaa atcttgttat ctaacctgtg aataaataca
     9781 ttcagtttct gcttttgata agaaatagat tgttctaacc gtttttctgc ctgttttgcc
     9841 actttgaact cattaaatct tccttcttct acattaaatc caaaagtgac tttatactca
     9901 cctatctctt gagctagcgg tgtttccatc atgacatgcc cttcatttgt cgtatctcct
     9961 gaagctcgcc caggtcgcca agcaagatta ggttttccaa gttcccctgt tgttgaaaat
    10021 aaggttatga ttaattgatt ttctatacgt tcatactctt ttaaaccatc tgcataaaac
    10081 gtaaaataat gatcattttc tgccacagag acactcttat cgaatatttc taaattaaca
    10141 ggtttctcta ccatagtttc tgaccaattc ggaggaatga catcaggggt attttcaata
    10201 aatccgtttt gaatttgtgc aattgatttt tcagatttat ctaaaacatt aattgctaag
    10261 cgcatacgat gcgagtaaac agtattatga attttgagtg tgccttcaac gatttgactt
    10321 tctcccagac tgaacgatat ttcataagat atttcttcca tttcgggttt ttcagataaa
    10381 cgatctttta aatctttagg aagttgagct tttccgtaaa caactaatgt ttcttgtaaa
    10441 gaatctttat atactttagc tgtttctaat tttagagata gttctgtatc tccttctaat
    10501 ggtgaataat catacgtatc gccatcattt gctgaatcga tcaaatgaac gaaatcaaga
    10561 tattgtctat ctccaactat caaattcaat tgcccatctt taaaagaaag tttatattta
    10621 ttgttactta tctcagtttt gtctatctcg attgctgttt ctaatttttc attatttttc
    10681 tcaaattcaa caacagtgta tcctagtgct ggcacattta catttatttt tatgtcaagt
    10741 tcataataag ctggttcatc aaaatattcg aaacctgtag caatcatccg ataagcattt
    10801 ttccgttctg gaaaataaac ttctttttca atgatattat agggactacc tttgaagtgt
    10861 attttctttg aatcagctac tacatgcacg attttcgatc cactataatt ctcagtcgtt
    10921 gtgttgaaaa caaggacttc attcgatttt agctttaatg catcagccat ccgttttaca
    10981 ataaggtttt cgattccgtc agctatctca ttagcttctt tgattcgatg gaaaatatct
    11041 tccgcaacat tatctgaaac acagccacca atactatcat gagcctggtt ttgcaaaatc
    11101 tttttccata accgacgaag taggccttta ctgatatgga taccatttct ttctgcaatc
    11161 agcatgagcg gctcaactcg ttttagaatt ttttgttcca atttaaaatt ttctattttc
    11221 atttttgttc ttactgagcc aatagaacga tgaacacgag agtaaaccgg ctgacgaagt
    11281 tcacctttat attcaggtaa ttcttttttg tttctgataa cttctacata agaaggataa
    11341 tctgaaataa tattgtggaa tttactgtat ttattcaact tctctacgac ttgatcaaaa
    11401 tttaaaatca ttgacttttg atctacaccc gaaggtacta aaatatcttt gttttttcct
    11461 tgcatatgaa gcatttcaac tgcttcatcc aacttttctt ttgcaaattc aggaatatct
    11521 ttaattgcgt ctaaggtcat cattccagtc atataaccaa atggaaaatt catggcataa
    11581 acaaatttat ctcccaatcc tttccagata aaataaggag atggaaccat cgtttcaaag
    11641 tttaatcctc gccaacacat gaaattatcg atttctactt gatgcaaaag tgtaggcaaa
    11701 ttcgcattga acccaaacgt gtcaggcaaa taccctacca tcattggatc tccatattta
    11761 aggatcgtat cactgattcc aatcattaag tttcttaaaa ttgattcagc atccactaat
    11821 aaggcatctg tttgtgcata ccacggtccc acgaatagtt gtcctgtttt catcagtcgt
    11881 tggataacct taagcttttg cggattgatc tccaaatact catcgacgat tgaagtttgt
    11941 ccatctaaac aaaagtttac tttagggttc ctttccaatt catctagaac ttcagtaaag
    12001 acctgatcag acaatacgat ggcatcttca tttgtaaaat accattctct gtcccagtgc
    12061 gtgtgttgaa ttatatgtgc atttaccata tcagattctc ctactttcct tcttctactg
    12121 aatttacttg ttgttctttt ttatacttga tggattttag tgcattgacc attaacgctg
    12181 taaccactcc cccaataaca attgatacta aatataaagg tatattcgta acaaaaggaa
    12241 ttgccataaa ggttgaaagc attgtttctt gagtaacacc aaaaaatcca tttagaccac
    12301 atgctactgc agtgcccacc acgatgcttg gtattactcg taaaggatct ttcacggcaa
    12361 acgggatcgt cccttcagta atccccacaa ggcccataat catagctgtc tttgcattat
    12421 ctcgttccga ttcatcataa agttttttgt ttaaaattgt tgctaaaaac attccaaatg
    12481 gcggaatagc aattcctaat aacatggctg tactaggtcc aaagcttccg cttgaaaaag
    12541 aagcaacccc aaaagcatat gcgattttat tgataggtcc tcccatatca aacgccatca
    12601 ttcctcctaa aatagccgac atcaaagcag catttgctcc tgacatgcct tctagccaat
    12661 tagtcagtcc tgttgtcatt gcttgaatag gcgtagctag tacataccat aatatgattc
    12721 caacaactaa tgtagtaaca aaaggaatca gcataattga ttttagacta ataacttgca
    12781 tcggtacttt tacttttttc aatgcattag ctaaatagcc acaagctaat ccagcaacca
    12841 ttcctccaat aaatcccata ccaagatttt gacacatcat cccagtgata aaagctggtg
    12901 ctagaccggg acggtcagca attgaatatg cgatataggc tgaaatcatc ggtacgataa
    12961 actgtaatcc cgtctgacca atcattcgca ggactcccca gaatgtatcc gtctggtccc
    13021 aaacacctgt gccaccaaaa atgactgcaa tagaagttag taaccctgct gctacgacaa
    13081 gcgggatcat ataggaaacc ccactttgga aatgatttag aatttctctt ccttgtttct
    13141 tgaattccat tagtaactcc cccacgatat attttttatt tgttaattaa tgcaattgct
    13201 tcatcaatcg tctcttcacc atcttttact acctctgagg tccctactgt taaaatcggt
    13261 ttaccgtcaa agcgacctcg ttctgaaaca tttgtatcta ctgcaaatat gacaacatct
    13321 gcctcggaaa catccgaaat tgaaagtttg ttttctatcc ccatagcccc ctgtgtttct
    13381 actttaatat ctaacccttt cttcttcgca aacttttcta aattagcttt tgccatataa
    13441 gtatgggcaa tccctgcgat acatgctgtt actgctacta tttttgtcat atgaaattcc
    13501 tccttttaaa atgatagagc ttttaccaat tcttttttat ttctcgaatc ttttattctt
    13561 ttaacaaact cgtcatccat caatcttcca gataattctg ctaaaatacg taaatgatta
    13621 gtacctttat cagtgtcttt tattgctaac aaaaagacga gatcaactgg ttgatcatct
    13681 aatgcgcccc attcaacaga ccgctttaac ttggctacag ccactgttga ttcaattact
    13741 gctaggcttt tgccatgagg aatggcgata tttttcccaa ttcctgtggt catatggttt
    13801 tctctgtcaa atacgtcttt ttgaaattgt ttgtggtcag acacaaaccc attagtctct
    13861 aacaatttcg ataatttttc gactacactt tttttatc
//
LOCUS       DAZULY010000056        13857 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000056 DAZULY010000000
VERSION     DAZULY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.057"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..163)
                     /locus_tag="ACG81A_001082"
     CDS             complement(<1..163)
                     /locus_tag="ACG81A_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFS5014465.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISAD"
     gene            complement(642..1361)
                     /locus_tag="ACG81A_001083"
     CDS             complement(642..1361)
                     /locus_tag="ACG81A_001083"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS5014466.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1410..3056)
                     /locus_tag="ACG81A_001084"
     CDS             complement(1410..3056)
                     /locus_tag="ACG81A_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFS5014467.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3235..3825
                     /locus_tag="ACG81A_001085"
     CDS             3235..3825
                     /locus_tag="ACG81A_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5014468.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3917..5422
                     /locus_tag="ACG81A_001086"
     CDS             3917..5422
                     /locus_tag="ACG81A_001086"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFS5014469.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5506..6858)
                     /gene="celB"
                     /locus_tag="ACG81A_001087"
     CDS             complement(5506..6858)
                     /gene="celB"
                     /locus_tag="ACG81A_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS5014470.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6858..7376)
                     /locus_tag="ACG81A_001088"
     CDS             complement(6858..7376)
                     /locus_tag="ACG81A_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014471.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7392..7718)
                     /locus_tag="ACG81A_001089"
     CDS             complement(7392..7718)
                     /locus_tag="ACG81A_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFS5014472.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7731..9710)
                     /locus_tag="ACG81A_001090"
     CDS             complement(7731..9710)
                     /locus_tag="ACG81A_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5014473.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9731..10045)
                     /locus_tag="ACG81A_001091"
     CDS             complement(9731..10045)
                     /locus_tag="ACG81A_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFS5014474.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10213..10506
                     /locus_tag="ACG81A_001092"
     CDS             10213..10506
                     /locus_tag="ACG81A_001092"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFS5014475.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10584..11696)
                     /locus_tag="ACG81A_001093"
     CDS             complement(10584..11696)
                     /locus_tag="ACG81A_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5014476.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11849..12697
                     /locus_tag="ACG81A_001094"
                     /pseudo
     CDS             11849..12697
                     /locus_tag="ACG81A_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13050..>13857)
                     /locus_tag="ACG81A_001095"
     CDS             complement(13050..>13857)
                     /locus_tag="ACG81A_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5014477.1"
                     /translation="GYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSG
                     EVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPI
                     EFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLT
                     TGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVAD
                     SSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4326 a         2827 c         2370 g         4334 t
ORIGIN      
        1 catctgctga aatatgaaaa tttttgaatg ctacgacatt cgccattaca ggtatgtaaa
       61 agaaaaaaat catccaaagc atacccggta cagccatcag gacaagtgct ttataacgcc
      121 atagattcat aaaaaaatga ttgaacccct ttcgtttttt catctaaaaa cccctttcga
      181 ttcattcgca acgtaattat agcgctttca ataatgagaa taaataaaca aagtataggg
      241 aaaatgaaac aaagtatagg agaggttgtg aaatcagcgt tttggcctga gtattagaga
      301 aaaaaacggg aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag
      361 ctgattgaac accgtttatc agaggttgtg aattcagcgt tttggcctga gtattaggga
      421 gatcgtgtcc ttactatcta actctgagtc acaaggaaca atggggactg taacaaaagt
      481 cttgtcacag tcccttattc cgaacaaacg gtgttccaga agtagcttct acggaaataa
      541 gccgaaattt cacaaaaaag ccatccgaaa gtcagacgga caagtctgct tttcagatgg
      601 cgctacaact actctgctta ttcttttagc tatccattca attatttttc tctcacttgt
      661 tctagctctt ctttggtcat gggtcgatat tgccctttat tcaaatcttt aggcagccaa
      721 aattttccca tacggattct ttttaaatat aagacttctt ttcctactgc tttgaccatt
      781 cgcttaactt ggtgaaactt tccttcatgc aaaatcagac ggataattga agtttctgtc
      841 tcttgatcta cttgatcaat ttggagtttt gccggcaatg tctgctcatc gccatcaata
      901 atcagtcctt ctgcaaataa acggatatct tcttctgtca ttataccgct aacctcagct
      961 agatattctt tctccacatg ttttttaggt gatagtaact gatgggcaaa caccccgtca
     1021 ttcgttaaaa tcaagaggcc ctctgtgtct ttatctaatc ggccgactgg aaaaagatct
     1081 tcccgataat cctggtcttc caataaatcg attacggttt gatcatgatt atcctgagta
     1141 gcagaaatga ctcctgcagg tttatgcaaa atgtagtaaa aatctttttg atacgtaatg
     1201 atttctccta aataagtaac cgcatcttcg aattcattta cttgcgcttt atcgcttttc
     1261 accacttgtc cattgacttc gatcgttcct tttttgatta atcctttgac ttctttgcgg
     1321 ctgccaagtc ccatatctgc taaaaactta tctaaacgca tgcgtttccc ccatttcaaa
     1381 agagcatcaa tctagctctt ctgattcact tattttattt ttaatttatg tcgtaaacgc
     1441 gctattcctg gtcctagtag tctttctgcc aagcggattt tcaaagctaa ataaccatac
     1501 actaaagccc ctatacctgc tactacaaga atcaacaata gagactggaa tttgctatct
     1561 tcgtttaaaa aagcaccaaa aatcatcttt gtgatccacg aagccaaaag cataatcaag
     1621 gtaagaataa agatcagcaa tgtcctgcgc cagacaaaac ttggtttaaa gcgggctact
     1681 tcatggattc tctttaagat caaggtacaa gacacagcaa atccgatcat tgtcgccaat
     1741 aaagggccgt atacttcaaa taaacgaatg gctgggtatt gaagaaggag tttcactaga
     1801 aacccaataa ccaaataatt aattgccgcc ttgttttcaa acattccttg cagcatgttg
     1861 gaaactagca tatatagtcc taggaagagt cctacaaaac ttgcttggat cagtacatta
     1921 cttcctagag aatccggtgt ataaaataat gtatttagcg gataggcaag taacataaca
     1981 ccaaatgtcg ccggaaacat aaaaaatgaa aacaactgta aatttccgct tgtcaatttt
     2041 gccagaccta ctcgatcttt caacgtaaca gcttctgtaa tcaacggtaa cccagtagac
     2101 gcgatcgatg ttgccaatgc aataactacc atcgtcaact tatctggatt ggcactaaaa
     2161 atcgaaaaaa gatccagtaa ctgtgcattg gaatactcgg tggatgcaga catgatcttc
     2221 ataaaagtga actgatcgac taatttgaag atcgttaccc ctgaacccac aataataaac
     2281 ggaatcgctt ctttaacggt atctaacaac aattccttcg ttgcaatatt tattttgttt
     2341 tcgctgtagt gaataaacgc cgatgtatac gcttgatgtt tcttcaaaaa atacaaaagg
     2401 acaagaatac ttgctagcat accgataaat gccgcgaaag ttgattgcgt gaccgccgtc
     2461 acatagtcac catccatgac tttcatgatg atgaatgtag ccaatagcat ataaaaaata
     2521 cgtgccacct gttctacaat ctgcgacagc gcataaggca tcatttcttg attgccttgc
     2581 aaataccctc gtatcacact catacaagga aaaaccaata cagcgatact caaagaacgc
     2641 atcgttggaa tcagttcttc tccgccacca gaagcatgag ctagccaagg agaggcgatg
     2701 tacatgaaca aagcaaacaa caggcctaac ccgcccatca tctgtaatgc acggataaat
     2761 aatcgtcgtg aagtgccata ttcattcaat gaattatagc gtgcagtttg ttttgcaatc
     2821 gcagcaggta ttcctgctgt agagatcagt agaaacaatg catatatatt gtatcccata
     2881 ttgaataatc cattggcagc cttagcattt tcgcccatcc aagcatacca gggaatgata
     2941 tagacagcgc ctagcaatct tgaaatgata ttactggcgg tcatccaagc agagcctcgt
     3001 gccatttttt cttgcggtgt taattgatct aatgctggtt ttctttgatt atccatcggt
     3061 cagattccaa ctttctacaa aacattacta tcattttaat tggtaaaaca gaaactggca
     3121 atcgatttta tataactttt ataagaacaa ttgttttttt gaattcggtt tcgttttttt
     3181 ggtaaagtaa agaaatatac agccttttat ggctaacatt tataggagat atcaatgaca
     3241 caagaaataa aaaaactacc cgttatattt ttaggtcttt tctttatcac agttggattg
     3301 aattggttct tgctccctca cgatatagct tccgccggag tgggcgccat cggacattta
     3361 gttgaaacgg ccttttctat ccaacgctca gttactgttt ggagcatcaa tcttatgatg
     3421 ctgcttctag ccgctgtcct attaggaaaa tcagtatttt ttaagactgt agcgggaagt
     3481 ctgctttttc ctgttgtttt agagattgtt ccaaaagtgg aaatgatctc ctcacatttc
     3541 ttttcactga tttcaggaag tcttttgttc agtcttgggg tttacacatt gtacacagtc
     3601 ggggcctcaa atggcggtat aacgataccg ccgattattt tccacaaatt ttttcggctt
     3661 ccaatgccgc aaggactttt acttaccaat agtcttatcg tctttttgaa ttattatgtt
     3721 tttggattgt tggagacact attcgttctc ctatctatta cagtcagttc attgtttatg
     3781 aaaatactca tccgcttgtc ccctgtgtca agaaaatttg aataaagctg gattttgtca
     3841 tagcggaaac aaacgatata ttaaaaagag tgtgctataa taaaccaaga aattgaataa
     3901 agcgagggat caatcaatga gcttctcttt agaagacatc cgtcatttac tattaaaaga
     3961 aaatctatta aaagaatttg tttcaacaag aggctggcat cttgatgtac cagaaaaagc
     4021 tgaatttctt cagttaagtt atgattcaag aaaagctgat gcttctactt tgtttttctg
     4081 taaaggaatg aatttcaaag aagaatattt ggattcagca atcgaacagg gtattcaata
     4141 ttatgtatca gaacaaccat acgaaaattc agcagtagga atcatcgtaa cggatatccg
     4201 taaagcaatg gcgcttcttg ctatggcttt ttacgactat ccgcaaaata aattgaaagt
     4261 gatcggattc actggaacaa aaggtaaaac aactgctgcg tactttacaa aagcaatctt
     4321 ggatcacaca acaaataaaa aaacagcatt gctttctact atgaatacaa cgttagatgg
     4381 caaaacatat ttcaaatccc atttgacaac gcctgagtca ttagatttat atcgtatgat
     4441 gtcagaagca gttgaaaatg gtatgactca tctagtgatg gaagtttctt cacaagctta
     4501 caaaacacaa cgcgtatacg gcttgacttt agatgtcgga atcttcttga atatctcgcc
     4561 tgaccatatc agtccgatcg aacatccgac atttgatgac tatttctatt gcaagcgaca
     4621 attgatcttg aattcaaaaa cagttgtttt aaatcatgaa agtgactatt ttgatttact
     4681 tagggaaaca gccgatctat tccagattcc aacgatcact tatggtcgtt cggaagaaag
     4741 caattatcaa gtgatccgtt cagataaagg tactcacggt tttactttat cttctcatga
     4801 agatcgctta gctatctgcg atactgctta tgatattttg ctggctggag gattcaacca
     4861 agaaaatgca gccagtgcga tcattgccgc agcactaaca ggtgcctcca gtcaagatgc
     4921 acaagacgga ttaaaagaag ctcgtgtacc aggccgaatg gatcaattga tccaatccaa
     4981 tggagcgcat gtctatgtgg actatgcaca taattatcta agcttgaaaa cattgctgaa
     5041 atttgccaaa aatgaacatc cagatggccg tgtaattgtt gtattaggca gcccaggaaa
     5101 caaagcaatc tcccgtcgac atgattttgg aaaagtgcta tcagaaacag ctgacatcgc
     5161 ctttttgacg gcagatgacc cagcatttga agatccgaaa aaaatcgcgg aagaaatcaa
     5221 tgaagcaatt actaatccag atttgatcgt tcattatgaa atggatcgtc ctgaagcgat
     5281 tcgacgcgct ttagcagaaa gcgctccgca agattcagtc gttattgcgg gaaaaggcgt
     5341 ggatccctat caaaaaatca atggtgtaga tgaaccttat gaaggggatt atgcaatcgt
     5401 gaagcggttg attgaagaat aatggtattt atatactagt ggtttcccat ttctaagaaa
     5461 aaaagggact gtgatttttt gtcacagttc cttttttctt agaaatcatt ttaacttttc
     5521 actagctaat tctgctgcct tttctctttc ttcatttaat aatttattat catagaattt
     5581 gataaatggg aaccagataa tgaatgcgat gattgcacaa atgactgcta acacagcagc
     5641 tttccaatca ccaccactac caacaaatgc tccgatcccg actggtgaag gccacggcat
     5701 ctgtgcaagc atcggtttga cgatttccag ccgaatggca aaataagcga gtgtagcaga
     5761 agccatcgga gctaagaaaa acggaaccgc taaataggga ttgtagacga ttggcatccc
     5821 gaaaatgatt ggttcattga tattgaataa tcctggtaca agtgaggctt ttcctaacac
     5881 tttcagctga tcagatttcg cgaggaatgc aataaagatg atcaatccta aagtcgcacc
     5941 tgatccacca acggttacat aagaattgtt gaattctcct gccaacggat aatttgcgcc
     6001 ttgtgcgtta gctgccatat tagctaatac gatcggggtc aagaaagaag tgatgatatt
     6061 cgctccatgg atccctacga tccacaatgc atggatcaag aaataaatca ccatgacacc
     6121 aagccatgta ttcgtcaact gcgtaacaaa cccaaatggg atagcaatca ttttgaagat
     6181 atccgttcct agtgcaacta ataatccatt gatgatcatc accacaaacg caatgacaaa
     6241 tgttgggatc agagcagtga aagaacgaga aactcctggc ggaacaacat ctggcatttt
     6301 tatgacccaa tttctcttga cacacataca atacaattcg accgcaataa tcgacataat
     6361 aatagctgta aagattcctg aagttcctaa acgttcgaca aaagagccca tcctaatacc
     6421 atttactaca gtttctgtct cattgataga agcaatcaag ctgattttac catcagtcat
     6481 gatcaattca gggatacaca taaagaatgc aaaaacagat agtaatgccc ccgtgagcgg
     6541 attaacattt aatttttgtt catctcgttc aattgacgtt aattcataag caaacacaat
     6601 ggcaaagtaa atagataaaa tccccatcgt cattgtattg acgatcatgt acaagtcact
     6661 aactttgaaa aaagttgcat taaaaaatcc ttctaaaaat gggaacggta atggcaaaat
     6721 atttaaaacc aaaaacatcg agcctacaat cgtgaaagga atagaagcca tccccgctgc
     6781 catgacagcc cgaacgattt taagttgcgc aattttcccc ataggtccca tcaggtattt
     6841 ctgcaaaaaa tcaaacatta gttattctcc tttcctaaat ataacgcttt ttcatattgt
     6901 ttaatacgga tatactgttg gtcttggttg agtgaaattt tctttaaacg atactgtata
     6961 aatgcactgg caaaaatacc gcttacaaca agaatgaaaa caaataactg tcctccattg
     7021 ttatttgcca taaaaggata caagaatgta aatcctgagg taaacaacac tggaatcact
     7081 aataaattaa agacactctg gaacaaaaaa taccactttg tccatggaac gttcgtttgg
     7141 tgttttcgat aaagctttac ttgttctcct actgcaggca aaatagctaa aatcagaatc
     7201 atcggtaaac tacttcctaa acttttagct gatagaagca gaatcatcca ttgcatgttc
     7261 atgaaaaaga aaaaggcagt caggtaacgt acgattagat aacgattgaa atacatgttt
     7321 ttcaaactaa gttgcttttt ttctaattcg attttccttt ttactgtatc ctgcacaatg
     7381 atccttcctt tctatttatt tttttgatac aaataattca tttcctgtgc aacttccaat
     7441 aatgtcatcg tcgtcatcag atgatcttgt gcatggacca tgataatttc catttctatt
     7501 tttgtcccgc tggcatattc tttcagaagt tgagtttgtg actgatgcgc atgaagaatc
     7561 tcttcatttg cttgtcgcaa cttctcttct acttcttcaa acatctcttt acgcatcgag
     7621 cgaaatgctt catgtatcag tgtccgtgca tttccgctat ggaaaatgat atcaaaagaa
     7681 gtcacttgta gtgtttcact gtttattttt tcagccatct gttttcctcc ttattcgatc
     7741 agtttgataa atttactgct gaaattctca aaaataggtt cagctaagat ttcctgctgt
     7801 acttttagct ggtcaactaa cctaacgatt gctttagtca cgaccgtaat tccttcattt
     7861 tcgacataag aaggcgaaac taaaaaaaca aaatgaattt cttgatattg atcccactgc
     7921 atcccagaag gaatcaatgc aacggctacc tttgtttcga ttccaactgg aacagcggga
     7981 tgtgggactg ccactgtatc actgaatatg atttgtccca tattttcacg ttgctggatc
     8041 tgctttcgca tttctcttat atatccttct tcttcattta tgctcagctt ttgcagcaaa
     8101 tcatgaagaa tttcttcctt tttcggttcg tacgtataag tcttgaaaaa tttttcagac
     8161 aactgttcgg tgtaaatctg ctttttttgc tgtagagaaa atgctggtat cggtctatct
     8221 gaggaatgat agggtataga ttctccaact acctttcgta ttttacgaac atcatcttca
     8281 ttcaaaaaaa tactgacatg taaaactgga attttgaaaa acattgtcga aagatcaata
     8341 gaagaaataa tcaggtcgat tccttttaaa gttgtttcat tgatctcata atagcctttt
     8401 acacttacca ctgtaatatt tttaccgaat tcactgacta cgcgattctt taatagttgg
     8461 gcactgccgt aacccgttgc acagataatc aatgcatgta gtttatgcgc agctctttct
     8521 ttttccaatg ccgccatgag atgcaaagcc aaataggccc attcatcttc attgatggta
     8581 tagtttttca aagccggcat gttgctgaaa tgccttttta ctgcgtcaaa gactttggga
     8641 ttttctttct ttatctcctc agtcaatggg ttctcaatgc gaatacctcg gctcaatcga
     8701 atcatcatcg gtgacaaatg atcgagtagt ccattgaaaa gctggagatc ctctactaat
     8761 gaaaaatgaa attcctgaga caatcttttc agaactttcg ctaattcctt tgatagttcc
     8821 tgatcatttt gattttcttt gtgattcgac ttagccatga gatgtaatgt caaataagca
     8881 atttcttctt cgggaaacga tacacctgcg attacctcaa tacgttgaac gatccgctgc
     8941 gccacttgat actctactcg cttagaaact tcttgttcga tgcctaattc cttgatttca
     9001 aacccttctc ggagccgttt gatccccaaa gccaagtgta aagtcagatt ctgtatgata
     9061 aaatccgaaa cttttaactt agcttctctt gtttcatcga gaatgataat gactagctct
     9121 tcaaaactga tgtctgggaa aaaacggctt tgccctaaat attctttcaa tgaatttcca
     9181 taatggtttc caaaaaaagt atccatgata aaatgccgtc gatttctttc ttccccttcg
     9241 atccatgtcc cttgaccatg cttagaaacg atttttagtg aatatggaat caatcgctcc
     9301 ttgatttctt gcatgtcctt tgtcaaactg gaacgactga tataaagctc ttctgccaaa
     9361 tcatccataa aaagaatggc atcttccaaa agcaattggt tcaatatata cttttgtcga
     9421 tcttcggact cataaaatcc ttgcggttct ctagcatatc caggtactat ttcttgccga
     9481 ctcaaaaaca agtcaaatgt cagtctttgt aaaatatgca gttggtagcc ataacctggt
     9541 ttagcaatga tcttacctcc gtttttttct actaactctt tgatccgctg caagtaatta
     9601 cggattgtcc gatcagataa taacaattca gaagctaaac gttgactggt gacatactcc
     9661 ttcttgtgat caatcaaata tctcaacagc aatttttcct tctgtttcat tatcatccac
     9721 tcctattcca tcatatgttc tcttcgatca cctctgctaa cttctggatg cctgtaggga
     9781 ttgggacata cgcttggaaa gggatactaa caacaggctt tccaacagat tctcctaatt
     9841 ttttgaattt atccaaaaac atcgtcgttt gcgggctgat cagaaataaa tcaaaatcac
     9901 tattcttaat catattatcg ccttctgtag ctgaaactgc atctacgagt acctcttctc
     9961 ttttttgctt aaagaaatcc gttgtctttg aggccatcat cgaggatgac attcctgccg
    10021 cacaaataat caatgctttt ttcataaatc aatcactcct tttattttta taaaccaagc
    10081 atatcttgta agcgttttct attcaaaata tatatttccg tagcattgcg gaaaaaatgt
    10141 gtatcatatc ttgaataata atttctggca ccgtgatata ctagtgatag aattttttca
    10201 ggaggggatt tagtgagtat cacagtgaat attttgtata caagtacaaa tggacaagcc
    10261 agaaaatttg cagaagaaat ggtcgaaaga aacattgtca aagcgattcg tgcagaagaa
    10321 ggaaacgaga aatatgacta ttactttcca ctagaagatc cagaatctct tttactgatc
    10381 gaccgttgga aagatgaaga agcaatcgaa aagcatcaca aatccgaaat gatggctcaa
    10441 ataaacgaac tccgtaaaaa gtataaatta aaaatgaaag tcgaacgttt cactgattta
    10501 ccgtaaaaag atacacatgg aatgatagca atttatcatc catgtgtatc ttcacttttt
    10561 ccttattttt tattcgatct catctaatga ttcaaaccgt gtttttctct tatttttcgg
    10621 ataagtattt cagtcacccc aatatacatg attcctaaac ataccccaag gatattctca
    10681 aaaatgcgga tcgttactgc tcctggaacc ccgaataaac tggcagtgat tgccaaagca
    10741 ccaaaagaat taaaaactgt tttatatcta taggtagaac aaacacctaa agctagacca
    10801 cctaatatac cgacccagtt aaaaggaata aattggctga taccaataaa taaaacacat
    10861 ccaatcaatg agccaatgat tcggtctact gctcgttctt tgaaaccaat cgacatcaat
    10921 ccatagctag atacaataga agaaaatgca aaccctgccc acatcaatcg ctgaaaaggg
    10981 acatactctc ctgcaacgat caaaagacta atgccaaatg catagatgag catccaaatt
    11041 ttttcctgcg aatacatgcc tttcaaaaaa atctctttaa acaagctttt gtctttgttc
    11101 ttttctcgat gttttctgta aaggagaact gaaaaccaac aaaaaaacaa aaataataag
    11161 ctacttgttt gtgtaaagaa attcttgttc aacgaatctt taggtaacga ataaacaata
    11221 aagagatatg aaaagccgta caaagaagca ttccccattt tgggatcact agcggttgcc
    11281 attaatatgc tgcttagtaa aataaaatga atcaacatgc ccataactga ccatttgatc
    11341 agctggacat acggtgctac taatagtgag aacatgacga ttgctaaacc tatgatcgaa
    11401 tgggaaatct tataaccaaa gtcaacaaaa cgaatactca tcaacataca gaataaaacc
    11461 acagcgtatg gtttatgctt cgattcaaat aatgttgtaa ttataatgat aaaacaaatg
    11521 gcaaacaata cgatgaataa tgatcgtaaa aacaatgaac aaatatacat gcacttttct
    11581 tttttagaag ctgcttctcg tattttttgt tttaaaataa acggatctag ttgtagagac
    11641 tgataaaaat ccacctgttg tcgtcctttc tttttacttt tattttttac agtcatctat
    11701 gacaggttgt aaatactatg gaacatcata tggcgttgtc attttcttgt caactatttt
    11761 tcctattcat gtttttataa aaaaacatat cgttttcttg tatgattaaa tcaccacaaa
    11821 ataaacaact gagagaacga tatgctttat gaatggttct atcaaaaaaa ttctgagatc
    11881 aatagacaaa aatttgatga ttacagaggt ctcttacgaa accttacaga agaaaaaaac
    11941 attgatcgtt gctgcagttc tcttccctgc tccatgtgtc tgtagaaatt gtggttctgc
    12001 tgtggtagat ggaaatagga aattaatggt ggtgaaaaac aagaagaagg aaaccattgt
    12061 ccactttgaa taatataatc atatgccaat ggttatacgc ctaaaaaagc agcgctatac
    12121 ctgtaaaaac tgtcgaagcc attggaacgc tcaaagttat tttatccgtc caaggcactc
    12181 gatttcaaat catgtcagac ataaaatcac ttctttactg actgaaaaag tgtcgttatt
    12241 ttttatttcg aaaagttgtt aggtatcttt gacaaccgtt attcgcacat tgaaagagtt
    12301 gagaagctat ttaccaaagt cttccaaaag aattctgtct tgagtgttga gggttgtgaa
    12361 tttcgttctc acgtttccgt tgaggataaa ctgagtttta tttccgcgga tggcgagaca
    12421 gaaaaattaa tcgagaaaat ttacctacac aaaaaattcc tcgattaaca aactatttcc
    12481 ttagctgcac taatcctaaa gaaatagaat tcttagttac agacatgaac gccacctact
    12541 ttcagctcac caaacatgtt ctaccaaatg cgaaattggt gattgatcga ttacttggtt
    12601 tttccacgcc tttcaaggag gcgtaacctt tattcacgag ttggttgaag ctttccgaga
    12661 caaaagaccc gacttgtttt tctccttatt gacaaagagc cattaaaact agagaaaaaa
    12721 ttacaactga tcgctgatta tttgtagaaa gaaaatcaaa gtattaccca ataaacttga
    12781 gtcattaggc aaaaaacact gcgaaactca agaatgattt tcgcagtgtt tttgtatatt
    12841 ttttattaac tagtattact acaaaggttt atttttcact tctcaacgca gcacaaaatt
    12901 gtctttactt atttactggt tgggttcaac cttctctact ccgatactaa tatttgtttg
    12961 tatacccctt ctaaaatcgg tagtttccat tcaattgatg ggtactagtt gattttaaaa
    13021 actatgctta ttcaattttt tattttagct catatatttc gtttctttct aaattttata
    13081 ctgatgaaaa caagtagcac acccaatatt gacaatataa actggctaat gatggtttct
    13141 ccagtttttg gaagatgctt atttccactg cttgttggat tgtttcctct tatatctgag
    13201 ctatcagcaa cttttctatt ctcttttgtt aaaaagatcg ccacttcttg ttctttttcg
    13261 atcttgaaag ttactggtgt cgcatccaat tcataccccg ttggcgcttg agtttctacc
    13321 agctgatatg ctcctggtgc taagccatct atcgccagtt ttccatctgc tcctgtggtt
    13381 agacctgact gcagaacggt tccattggca tcttgtaact caaaaacagc tccttgcagg
    13441 acttctccgc tttggtcatc gatctttgtc aacacgacac cgcctggtgt aagtcgattt
    13501 tctttcgtca attctaccac cgctgtctga cttctttcga tctcaaactc gattggtgtc
    13561 gcatccaatt cataccccgt tggcgcttgg gtttctacca gctgatacac tcctggtgct
    13621 aagccgtcaa tcgccagttt tccatctgct cctgtggtta gacctgactg cagaacggtt
    13681 ccattggcat cttgtaactc aaaaacagct ccttgcagga cttccccgct ttggtcatcg
    13741 atctttgtca acacgacacc gcctggtgta agtcggtttt ctttcgtcaa ttctaccacc
    13801 gctgtctgac ttctttcgat ctcaaactcg atcggtgtcg catctaattc atacccc
//
LOCUS       DAZULY010000057        13852 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000057 DAZULY010000000
VERSION     DAZULY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.058"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(221..1423)
                     /locus_tag="ACG81A_001096"
     CDS             complement(221..1423)
                     /locus_tag="ACG81A_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5014478.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="ACG81A_001097"
     CDS             complement(1426..1620)
                     /locus_tag="ACG81A_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS5014479.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="ACG81A_001098"
     CDS             complement(1667..2515)
                     /locus_tag="ACG81A_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFS5014480.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="ACG81A_001099"
     CDS             complement(2720..3070)
                     /locus_tag="ACG81A_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014481.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3248..4111
                     /locus_tag="ACG81A_001100"
     CDS             3248..4111
                     /locus_tag="ACG81A_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5014482.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="ACG81A_001101"
     CDS             4735..5727
                     /locus_tag="ACG81A_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5014483.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="ACG81A_001102"
     CDS             5743..6627
                     /locus_tag="ACG81A_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5014484.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="ACG81A_001103"
     CDS             6676..8283
                     /locus_tag="ACG81A_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5014485.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="ACG81A_001104"
     CDS             8284..9768
                     /locus_tag="ACG81A_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFS5014486.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="ACG81A_001105"
     CDS             9945..10937
                     /locus_tag="ACG81A_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5014487.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="ACG81A_001106"
     CDS             10989..11858
                     /locus_tag="ACG81A_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5014488.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..13843)
                     /gene="guaA"
                     /locus_tag="ACG81A_001107"
     CDS             complement(12278..13843)
                     /gene="guaA"
                     /locus_tag="ACG81A_001107"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS5014489.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT         4243 a         2343 c         2861 g         4405 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt tggctcttcg tcaaatcgtg ttggtgaaga caaatttgaa ta
//
LOCUS       DAZULY010000058        13852 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000058 DAZULY010000000
VERSION     DAZULY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.059"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(195..1487)
                     /locus_tag="ACG81A_001108"
     CDS             complement(195..1487)
                     /locus_tag="ACG81A_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5014490.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(1484..2107)
                     /locus_tag="ACG81A_001109"
     CDS             complement(1484..2107)
                     /locus_tag="ACG81A_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014491.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(2110..2946)
                     /locus_tag="ACG81A_001110"
     CDS             complement(2110..2946)
                     /locus_tag="ACG81A_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5014492.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(2963..3850)
                     /locus_tag="ACG81A_001111"
     CDS             complement(2963..3850)
                     /locus_tag="ACG81A_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5014493.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(3858..5093)
                     /locus_tag="ACG81A_001112"
                     /pseudo
     CDS             complement(3858..5093)
                     /locus_tag="ACG81A_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(5776..6459)
                     /locus_tag="ACG81A_001113"
     CDS             complement(5776..6459)
                     /locus_tag="ACG81A_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS5014494.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(6477..7094)
                     /locus_tag="ACG81A_001114"
     CDS             complement(6477..7094)
                     /locus_tag="ACG81A_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFS5014495.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(7104..7928)
                     /locus_tag="ACG81A_001115"
     CDS             complement(7104..7928)
                     /locus_tag="ACG81A_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFS5014496.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(7921..8481)
                     /locus_tag="ACG81A_001116"
     CDS             complement(7921..8481)
                     /locus_tag="ACG81A_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014497.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            8655..9611
                     /locus_tag="ACG81A_001117"
     CDS             8655..9611
                     /locus_tag="ACG81A_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5014498.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(9916..10872)
                     /locus_tag="ACG81A_001118"
     CDS             complement(9916..10872)
                     /locus_tag="ACG81A_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFS5014499.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            10976..11578
                     /locus_tag="ACG81A_001119"
     CDS             10976..11578
                     /locus_tag="ACG81A_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5014500.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(11666..13165)
                     /locus_tag="ACG81A_001120"
     CDS             complement(11666..13165)
                     /locus_tag="ACG81A_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFS5014501.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(13181..13705)
                     /locus_tag="ACG81A_001121"
     CDS             complement(13181..13705)
                     /locus_tag="ACG81A_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFS5014502.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
BASE COUNT         4009 a         2984 c         2422 g         4437 t
ORIGIN      
        1 ttattccttt ttgttcgggt ttaaatagtt atttttattt tctcctatct aaaaaagcag
       61 cccacataaa aagaaggcat gacaaaaagc atgtcatagc ccctttttcc gaataaatag
      121 tgttcaaaag acagcctatc ggcaataagg caagatttta taaactacgg gaagtaattt
      181 ccgtaaatct attcttattc ttttaagctg acaagctcgt caatcactac tgcttttcct
      241 gaagcaatag aatgatttgc tgcgattcct gtcaaaatcg acaaaatacc atctacgtga
      301 gttgctgccc gattccacgg atcttcttct ttttcttcac caaatagatc atctaataaa
      361 cgcggatctc ctccgccatg tccgcccgct tcttgttcga cgattacctc atatggctca
      421 ccgaacatcg gatagacatg gatggactga agaacggctg ctccttcatc ctttttatct
      481 ccacctgcat tgatgtaaga cttttcgatc actttcattt cgatccgtcc tttgctgcca
      541 ttgaaagaaa cattgaatcc ttcccacggc atatacgcgt ttagcgaata cgtcaaaatc
      601 gcaccagttt catatttgac cgttacgcct agtgtatctt caatagagat atcatcggaa
      661 aatacactgc ggtcacgaat atatccactg tctttttctg cttctaaata catttctcgc
      721 aattcctcat tggctgacag gtctatagca aatggatcag ctttagctag ttcgcttcca
      781 tagcatcgac tgtaaaatat ttctactccg cgtttctccg cattatgcat cccataaaag
      841 tttaatccac cgaatgcaaa taccttttgg ggacggctgt tcaaccaaaa attcactaga
      901 tcaaaatgat gggtagattt atgaacaaga agcccgccgc tattgtgctt atttcgatgc
      961 catcttctga aataatctgc cccgtgttgc gtatttaaca gccattcgaa gtggatagaa
     1021 aacacatcac caatcacatc actttctatc agttccttta tcttcgtgtg atacggtgca
     1081 taacgataat taaaagtgac tcgcaggctt cttcccgtct gtttttggac cgttattatt
     1141 tcttgagctt tttcctcatc gatagtcaga ggtttttcgc aaataacatc atatcctttt
     1201 ttcattgcac gaatgatata atcatgatgc gttcgatcta ctgacgtaat aatgacttca
     1261 gccggccgta cgtcatccag tagttgctca aattgtgatg gcatacatgt ctttactttt
     1321 ttctgtccat acttgttcac aagtatccga tttgcataat ccatccttgt ttgagataca
     1381 tcacaaaaac cgactagttc acaagtttga tgatacgtag aggcaattgc ctcataaaaa
     1441 aaacgcgctc tcccaccaag tccgacttgt acatattttt tcttcattta aagtcattct
     1501 ccttttcttt taagcaaaat actgtgacaa atggcagccc tggatacagt aatgcagtga
     1561 gtaaaaaatc aggctgtata caccagaaca gtccatacaa aaggcataaa taccacagaa
     1621 accccttgac gttttttgac aacagccaga tattcctttt gatcaaataa acaagactat
     1681 ctgctttatc ttctttgcag atatcaatta gcgtactaaa aaaactatgg gatacaaaaa
     1741 gcaacagaca caatagaaaa ggaatcagat accaagctcc aatctttccc actaataaat
     1801 accaatcaac tgcaaaaata atgacaaaca gctgaaacaa cgtaatgatg aaacacatcc
     1861 gtttgatttt ccctttcctc caacatacct ttttttctgc caagtaatgg acagatagat
     1921 aataagaagg atacatcatc aaaaaataaa tcagatagac aacacgagca gacagcacta
     1981 atcctaaagt gaacaaacat cctagaacta aagcgaaaag agcattactg ataagactgg
     2041 ttaaaaaaat ccgacagaca tagtagttga gcttgtaaag aaacatctct tctattggct
     2101 ttttcatatt cagcccttca gaccgcttgt actgatcccc tggacaagat atttattgaa
     2161 gaataagaaa atcagaaaaa ccggtagcag cgacaacgta gacatcgcaa acatactcgc
     2221 gtaagtcgta ctagatccag cgtctgcaaa taatttgatg gcaatcgata ccgtgtaaga
     2281 tctcggcttg tttaaataaa tcaatggtcc catgaagtcg tcccatttcc aataaaactg
     2341 gatgatgatc gtcgtgatga tcgaaggttt cagcaaagga aaaataattt tggtaaagac
     2401 actatacttg ctgcatccgt caataatcgc tgattcatct aactctttgg ggatgctgac
     2461 catgaattgc atgatctggt aaatgaaaaa tgcttgtcca aaaaaatgtg gtaaaatcag
     2521 cggcacatac gtcccaacca aattcagccg attgtaaata ataaactgcg gaatcaaaat
     2581 cacttgtgca ggaatcatca tcgttactaa catgacgacg aaccagaatc tttttccttt
     2641 gaaatcgatt ctcgataatg cgtaagcaat acaagtcgag ctcagaactg tccctatcgt
     2701 cgaaactgca gagatgacaa acgaattcct aaagaaagta aaaaatgaaa ttccagcaaa
     2761 ccccgcccat ccttttgcgt aattttccca ttgaaaagtc tctggaatca aattcagcga
     2821 accactcata atctcttgat ccgttttaaa agaacctgcg atcatccata agaccggata
     2881 aatcatgact agacctaata gcaaaataaa accgtattga aaccatttcg ttactgtatt
     2941 attcatttct tcctcctctc attcacacaa cgacccattt ttttgatgat ttgaaaatca
     3001 gaaaggtgac agctcccata atcaacagca tgatccacga aatagcacta gcatacccca
     3061 tagaattaaa agaaaaagct tggttataca cataaagagc ataaaagttc gtggcatcat
     3121 tcggaccacc ttgggtaatg ataaatgcct gcgtaaaagt cataaacgcc gcgattgtct
     3181 gcataaccaa gttatacaaa atgatcggcg aaagaagagg taacgtgatc ttgtaaaacg
     3241 tctgccattt gttcgctcca tcgatctctg ccgcttcata atacgagcct gggatctctt
     3301 tgatacctgc agcaaagatg atcattgccg aaccgaattg ccaagcagac atcaaaacaa
     3361 gagggatcat gaccagtttt tgatcgctaa accaggaaag cgcaggtgcg cctaatcgtt
     3421 ccaggaaaat attcactaac ccgtttttag aaaacaactc ccgccaaact agagcgatag
     3481 aaatactgcc accgatcaaa ctaggcacat aatacaaaga acgaaagaaa gtcgttcctt
     3541 tgatattcct cgttaaaaga taagcaatca atagaccaaa gatcagtttc acaggtacag
     3601 atgcaaatac atagagaaaa gtgactcgga tggagttcaa aaaacgggca tcactcagca
     3661 tctttatata attatccaag ccgatccatg agactggatt tcccaaacga taatcggtga
     3721 acgaataata aagtgacaag atcatcggta tcactgttat caatagaaat cctaggatga
     3781 atggagaagc aaatacgtaa ccgaccacat tgtcttgatc caagaaccgt tttagttttt
     3841 cttttttcat gccttccttc tgctgttact tcttcataaa tgactttttc aactaaaaga
     3901 ttgtattggt cttgaatttc cggattcaaa gcactatcaa agacattcac ttcgccgttg
     3961 tccatatcgg aaatcatttc taaataatca aatgtcgctt gcaaagttgc atcatctgca
     4021 cgagcgagct gttctcgaat attcgacatg attggtacac cacgttctcc ttttaaaatt
     4081 tcattggctt cttcactgtt ttgaaagaaa tcaataaact tagcagcttc ttctttatgg
     4141 gctgagcttt ttgccatact cagcatttgc gaagaacgta aagggatacc agattctcca
     4201 tctgcttttc tgcgaggtgg attaatcaac tgaatctcca tatcttcagg agcccctgac
     4261 aatatttctg tcaactggtt acttgataga aacaccattg ccgcttctct atccctaacc
     4321 gcctgaaaag acagatccgt cgtacttgca gctccaccac ctggttctgg atgggcccct
     4381 ttgtctacca gatttttacg cagctggaaa aaatctgtta gatagtctgg attttcaaat
     4441 cccaagctgt tagatagatc ttcccgatta taaaaattca atcctttctc aacttgtggt
     4501 atttgaataa tacagccagc tataaaatct tcgaatcttg aaatagcata gattcctaat
     4561 tcttcagtga tttgatagct gatttgttca aattcatccc atgtccaatt tgaagctggt
     4621 tcagggatat ttaattgttc aaatatattt ttattgtaag caatcgcaaa cgaattcacc
     4681 ccgttagaca gtccaactaa ttgcccgtcg taagtcgtcg ttgctaaata ggattcatct
     4741 gcattagaaa tatcaattat cccttgctcc acatattcat ctaatggttc aatctgattg
     4801 atgtattgag gaaattgatt gttcagttgg aaaatgtccc agatatcatc tgatgctgct
     4861 agtgtaccta attttgtgta atatccttca taatcataaa attcataatc gatcgtcaca
     4921 tggggatttt tttcttcata aagctcgatt acttttactg ttgcatcgtg tcggacttgc
     4981 gaaccccacc aagccatgtt cagcgtgatt tttttgtcat cttcttgccc acaagccgtc
     5041 aagaagaata acataacact taaagctaag atgattctca ttcttttctt cattctctgc
     5101 acctctactt ccattgttat ttttcaatat actgtatttt tatacagaac atatttatta
     5161 aacgatcttt ttcaagggga ataaaaacca agacttgcct tatcgttctg cctaggatga
     5221 acaactcaat actttcaaga aaaattagat agtgctgatt cttctttcat cgctaaaaac
     5281 acttcttgct tcaatgatgg gaggataatt acgcaaagaa aaaaggatat cagacaactg
     5341 cccaatatcc ttgacatagc taaataagca tgcttcttcg tgtgcgctct tccctaaaaa
     5401 acagaaaaat aactagtaat tctgtatgaa attaggtaca atatacacgt cgatgcacaa
     5461 gcattgacta tggcaacttg attggtcagt tcccgctgac ttttcaagcc ccgacaattt
     5521 agctgctact aaattgtcgg gtgccttttt tagtttttat tcgtttttca ttctatagaa
     5581 agcgcttcaa taaatcaaga catttttttg ttagaatgct aataataaca aaaggtttta
     5641 actcttctta tgtacattac aggtaaaaaa acaaaaaaat ttctcttttt tttgaattct
     5701 gaacaaatag tactggagta caaaagacga ggaagagcca ctatctgctc ttcctcgtct
     5761 tttgattctc acctgttatt catgattctt tttctcagtt tcccgatatt tttccatttt
     5821 acgactaaaa agctctccgc tacttggtgg cgtatatgtg atggcatttg ctcctgcttg
     5881 aatggtctca gcaatacttt cttccgttgg accacctgtc gcaatgatag gaagttcagg
     5941 atatttttta cgaaaatggc gtactgcttc tgcagtttct ttacctgcac tgatattgat
     6001 aatcgaaata ccagctgcta acttttcatc tatttctgta tatttcgacg ttaccgtact
     6061 gataactgga atatcaacta ctcgacaaac ttcttttact gtctctaaag gcgtaggacc
     6121 gttcaaaacg acccctaacg aaccttgcgc ttctgcgaac atgctcatgt aggaagaacg
     6181 agcaccttgt gtcaaaccac cacctacacc ggagaaaact ggaatgtcag ctgcctcaat
     6241 aatgctttta gtaatcgctg gatgaggtgt aaaaggatac acagcaatta ctgcattcgc
     6301 atttgtatta cgtatgatcg caatatccgt agtaaagatg atcgaacgga tcaattttcc
     6361 aaatatcttg atcccgcttg cttctaaaat gatttccggt actcttacga tatcttttct
     6421 caattccgtc atgatttctg gcgtaaattg ttctgccatc agccacttct cctttgctat
     6481 ctccggtcat actgattcaa ttgatacgtc tcgatgacct ggatgatttt ttgcgcataa
     6541 ccggggtctg tcgcataacc tgcatcttgc aatgcttgtg cggcttcctg atagttggtg
     6601 gccgtaatca ctttttcata cagcgcagga ttccaatcta ctccttggac gaacagttgt
     6661 gtatggtcat ccatggactg ttcccatgaa tcatacactt tgaagtctcc ttggatagtt
     6721 atccattctt cgttaacaaa ttcctttgtt tccaggctta ctttcttctg atccccataa
     6781 gccttgatcc cgaaaagatt attgtatttg ctagccaagg tactttttcc ccaatttgat
     6841 tctaatatag cttgtcctaa aatgatacta ggaagcactc cataaccatc ttgtagttct
     6901 tgggcatgcg gaacaagccg ttcaataaat tgctgatggg attgcgcttc attttcttgc
     6961 tgattatcag taaaaggaga agacagattc ttcaaggaaa aaacaaaggc taagagtatc
     7021 actgctacac cggcaaccac tgctggccaa cggatattct tgcgcttttg ctttttccac
     7081 ttttttttcg ccatgatcaa ttcctacttt tcatttaatt cattcagata ttcttctagc
     7141 gtcaattggt gtttggtgat atactcagcg ttttctttac cgacataacg taagtgccaa
     7201 ggttcatatg tgatcttagt tatatcttcg tgtccttttg gataacgaac gataaatcca
     7261 tactgcgcag cattatctgc gatccattta gctccaggct gatctccgta acttgcatcc
     7321 aatacagtag aaggataact gttgtaccag ttttgatcga cgacatcgac tgccagacct
     7381 gtgtggtgtt cgctgtatcc tggctccgta atcgtttctt tagtaatctt tgtcgcctcc
     7441 tcttctgata caccttgacg actcatcaca tcttggacat tttgttcgaa aacttgttgt
     7501 tgggatgata cagaccggta agctgagacc atcactaatg gaaatccagc tgcttcagca
     7561 gccttggcaa atgcttcata ttctggagca atgcgtttgt cgatcaagta tccgttactt
     7621 aatgatgcca attgagtaga ctcatcaatt tctttttcta atttgtgatc tggaccaacc
     7681 aaaactagct tccaatcgtc tgttttaaca tctggcaatt gtttggacgt acgatctttt
     7741 gtttctttgt cctttacctt gctgctggtt gtttgactgt tcttttcttt actagcagtt
     7801 gttttggtgc ttgttcgttc gactgcttga ttgccggctt gaacatgcga atcatcatcg
     7861 ctaaaaataa catagcccat tgctgcgatc ataatcacaa ctgctccaaa tcctaaaaat
     7921 ttattcactt gaaaaatcct ttcgttgttc ttcacgttcc ccattcgcct catgaacgta
     7981 agtttcttcc agattttcat tcacccaatc gagaagttcg attttttctc cttcgatctg
     8041 tttttgaaca tctgctggca gacgaaaatt caaaatatca tcatagcccc attcatcata
     8101 ttgaatactt actttcaagt caaggtatct accagcctgc tgattatttt tcaaagggat
     8161 cagctcgact tttgctgctt tgctgctaag ccatttttgc gcgatgcgtg ttttgatttg
     8221 cttgtactct gcaacggcaa cgtttcgttt ttcgggaaaa ataatcgttt gacgcaaaat
     8281 cttttgcgtt agcatccatt cataatcagt gaaaagattt tttactttct gcatcgcttc
     8341 agatggcaga tttttctcgc catctttcca tttttgccat tgttctggac tgatcagcaa
     8401 gtattcatct aaaaaatttt tttcagtttc aaactgttct aataatacac tcataataag
     8461 atcaatatat acttgatgca cgtttatccc ctccttttaa ctattctact taagttttga
     8521 tcaaaaagca cctcttaaat cgctgctttc atttttttta atgtttcaag tcctctcctc
     8581 tttattcttc gtgatttttg ttataataat agacagacaa gaaactcaat ccaactatga
     8641 aggagcgttt tcagatgaaa aaatatcaat ggggaatcgt tggtcttggt accatcgcac
     8701 atgagtttgc cgaaagcttc aatcaagaga cgagcgaact aacagcagtt gcttccagaa
     8761 cttcagaaaa agcggaaaac ttcgctcacc gttacaatat tccaaaagct tacggaagct
     8821 atcaagaaat gttagacgat gctgaaatcg acatcgtgta cattgctgta ccaaatagac
     8881 agcatatcga ccatatcttg gcagcattgg aagcaggcaa acacgttcta tgtgaaaaag
     8941 ccatcacgat gaataaaaaa gaactggctg acgctatgcg cttagcagaa gaaaagaatt
     9001 tgatcttagc agaagcgatg acaatcttca acatgccact ttatcagcag ttacgctcaa
     9061 taatggatac aggaaaactg ggggctttaa aaatgatcca agcacctttc ggcagttaca
     9121 aagaaccaga ccctaaaaac cggttcttca atccagaatt agctggagga gctctgttgg
     9181 atatcggtac gtatgctgta tctttcgctc gtttcttttt gagctctcaa cctgaagtag
     9241 ttgcctctac aatggttcct tttgaaacag gcgtggacga acaatccgtc actatcttgc
     9301 gtaacaaaga aaatgaactt gctgctgtca gcttgacttt tcaggcaaaa atgccaaaag
     9361 tcggtatcgt tgcctttgag aacggttatg tgacgattgc tgattacccg cgtgctgatc
     9421 gtgcggagat cttatttaca gatggtacaa aggaattcat cgaaagcggg aatacatctg
     9481 aagcaatgaa ttacgaaata aagaatatgg tacacatgat tgaaggtgac ttgcccaatc
     9541 gctctctctt cttgacaaaa gatgtcatcg aaatcttgga tcaaatgcaa gttctatgga
     9601 aaaatatgta attttaaaag gttgtgaaaa agctgttctg catcaagtaa taagaacagc
     9661 caaacaaaaa tagctcctcc tatttttcgt ttggctggac ttattaccgc agaggaaagc
     9721 ttttgaacac cgtttaagaa agaggttgtg agcctaccgt tcagctccga gtcacaagga
     9781 gcaatttcaa aaaacagctt ctcatgtttg ttgaatattg tgacttgtga ccgaggagtt
     9841 gacaggagaa caccgtttaa agaagaggtt gtgaaatcag ctttttgacc tgaatattag
     9901 ctgaattcac aacctctatt tttggatttt ttgttgggag aattctgcct tgatttcaat
     9961 aaaactttta aaagactgca aaaactggaa caattctgct cgatcctcga attcagggcg
    10021 actagtcggc agaggcattt gccgatacaa tacataaact tcttcaatac gtgttaaaat
    10081 ttgtctgcca tcatttgact ctgaaaatgt ttctgcagta taaatcaata aaccatagat
    10141 atgttttcca taataagctg gttgctggat tctctctaga ttttccagca tgtctttaat
    10201 cacattggcc tgtgctcttc tcatagagaa gtaagtttcg tagtatagcg gctgtctgag
    10261 ccagtagttt tcttgatgtt ctctggcatc ttcttggctt tcgcgaatga atgtcagtaa
    10321 atgttcgcat ttgatctgca catcttcttt attttctgat aaaagaaatt ctgccatttg
    10381 tctcaaaata tttcgaaatt ctttttcgat ctgtatttgg ttttccttca gctgcttcgt
    10441 acgatctggc atgtaaacat ttgcaagcaa agcaaatact aatccaatcg ccattaatcc
    10501 cgcttcattg ccgatcagct gccaagacat gttttcctcc actaaataat gagtgaccaa
    10561 aaccgaattt actactatcc cttcggttaa tttgaagcga gaggcaagcg ggatgaacag
    10621 gaataaaaaa agactgaagc caaaaatagt atacccaaca gcaaaaaaac tgaacagtgc
    10681 tgttgccgta gctaatataa aggctgcgat tcgattcatg cccgtacgca atgtagagcg
    10741 cttggtattt cctacactta ataatgcaat gatccctgca gctggccaat acaacagcga
    10801 taagctaccc gctgtgacca tcgaaaagaa gacactgatc actgttttga ctgttcgcat
    10861 tcctatacgc atttacttcc ccctttttta ttttaattat acttgaataa tgaaaaacta
    10921 gctatcagac aaaagtttag gtatgatagt aaaaagacca aaaaggagaa tctctatgca
    10981 atttggccgt tttcgactag gtatgcgtac gatcaaaagt gccttagctg tttttttatg
    11041 tatcttgttt tttcatttga ctcaacgagg aatcccgatg attgctgcat tatctgctgt
    11101 tttttctttg cgtcaggact tgactacaac cgtttctttt ggaaagtcta ggatcatcgg
    11161 caatacatta ggtggtacat tagctatcat ttattttttt gtcaaaagct attttcacaa
    11221 tgattttttg gtagaattga tcttgcttcc tattctagtt attattgtga tcgtcgtgtc
    11281 agatggtatt aacaataatt ccggtatcat ttccgcaata gctacgttgt tgctgattgc
    11341 gttgagcatc cctcaaggcg aatccgcttt gtatgctatc caacgtgtac tagatacttt
    11401 tatagggaca ttcattgcaa tcggtatcaa tttctttctt cgtcctcctg aaacagaaaa
    11461 gaaggaagaa ctatcagaag atttggtcga actccaaaag aaagaacgat tgctaagaac
    11521 aaatttacaa gagatagaag ctaagatcca aaaacaaaag aaaaatagcg aggactaatt
    11581 ttcttttgca ataaaataaa aaaatggaga gaagaacttt aaaaagtctt cctccatttt
    11641 tgattgaacg gtgaagtgta ctactttatt cttccttcat cgagttttgt tctgcctctt
    11701 ttggcgcttc atacatattt acttggctag tagtcccacg tgtcgacaaa ttctctacga
    11761 tttccttcaa gtcaagtcct gttgtcgctt ttagtgtttc ttgagtagaa gaaagcaaat
    11821 ttgtggcata atttgtcact cggttcgctc cactggtttc tcctgaactg cttcctgtat
    11881 cgacaacaga gattttttcg atatttccta gcggctgtgc tgcttcgcgc atcaattgcg
    11941 gtagcatatc gataaccata ctcaagacag ctgcttcgcc atattccttg aaggcattgg
    12001 cgatcttttg ttttgcttcg gcttctgctt ttccttttgc tagtgctgct tcggcttctg
    12061 cttgtccagc taaacgtgtc ttattggctt ccgcttctgc cagtgcttcc acacggaatc
    12121 gttctgcttc ggcttcagtc acttctttga ctttttgagc ttgtgcttct tgttctttag
    12181 cgtaccgatc ggcatctgct tttttcttca cttccgaatc atattgcttt tcacgacgtg
    12241 tgatttcttt ttcttccagt tcgatttgtt tttggcgttc tacgatttta atctgcattt
    12301 cctgttcgat tacttgttgt tgtgctcggg cactttctaa attgtaggct tggtctgctt
    12361 ttgctttagc aacatcttgt tcttgtttgt aggttgctaa tttcagttct ttttctttta
    12421 atgattctgc gatctctgtt tgtcgctgta attcagctgc ttgtgattct ttttctgctt
    12481 gagctttttt gatacgcgtt tcttttagag cttccgcttc tgctatatct gcatctcttt
    12541 tgacttgggc gattcttggt ttccctaaag agtcaagata accgtttttg tctctgacct
    12601 ctttgatagt gaaagaaacg atgatcaacc ccatttttgc aaggtctaca gaagcgactt
    12661 cttgtacgct ttgactgaac ttatcgcgat tttgatagat ttcttctaca gtcatagaac
    12721 caagaatcga gcgcaagtgt ccttctaata cttctcttgc ttcattttcc agttcttctc
    12781 ttgtcttgcc taaaaactgt tctgctgctg tagcaatctc ttctacagat gaaccgattt
    12841 tgatgatcga agtaccatca cacatcaccg gtacgccttg ttcggtatac acttcagggg
    12901 tggagacgtc caatttactc gataatagac tgatccgatt cgaacgttga aaaactggca
    12961 agacgaatgc gccacctcca cgcacaattt tgattttgtt attgctttca tcaatgtgca
    13021 catttttgct tcctaaataa cttccactga tgatcaacgc ttcatccggt tttgctgtct
    13081 ggtacttcgc tacaaagacg atcagcaaca tcagtaaaat caaagctgcg attgcgatcg
    13141 cgataaaaac aactggtatt tccatttaac acacgctcct ttaagctttt atcatgcttt
    13201 cataaggcat gacataacag attctttcct tgatctcaat gatcaatact ttcgttcccc
    13261 gctcagctgt ctgttcttgg gggctgtaaa aacaagctgg acgactaatg gaaccggtaa
    13321 cacttttcaa ctgaatttct cccatacctc gtacagggat cggtgtgatc acaacagcta
    13381 cttgtccttc cagagttttt tcagaagacg acatcgttga ttcggcatgt ttcatcggca
    13441 tcaatatata gaaattaagc aaaaatacca acactatcga tatgcctcca ctagcaataa
    13501 ataaaaataa agatgattga tctgttaaac gctcacctag aaaaccaaat aagcttgtga
    13561 aagttaacca agggataata agcatcggat caagcgggcc atcaaaatcg aatacatctc
    13621 caaaaataat caataggact gccaatcctg cacaaatcag caatgtatac caataaatcg
    13681 tctcaagtgc gattccccct atcatataca cgcctccttc catttctacg tctatcatac
    13741 caatgtttga aaattctgta tagcttcttt taccgactca gtggtgtttt ttataggaag
    13801 gttgtgttta tcgttgacaa tttgttaaat ttttgttttc atgttaaaat tt
//
LOCUS       DAZULY010000059        13731 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000059 DAZULY010000000
VERSION     DAZULY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.060"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1452
                     /locus_tag="ACG81A_001122"
     CDS             <1..1452
                     /locus_tag="ACG81A_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5014503.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1801..2433
                     /locus_tag="ACG81A_001123"
     CDS             1801..2433
                     /locus_tag="ACG81A_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFS5014504.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3021..4460
                     /locus_tag="ACG81A_001124"
     CDS             3021..4460
                     /locus_tag="ACG81A_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5014505.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4755..4973
                     /locus_tag="ACG81A_001125"
                     /pseudo
     CDS             4755..4973
                     /locus_tag="ACG81A_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            5222..5437
                     /locus_tag="ACG81A_001126"
     CDS             5222..5437
                     /locus_tag="ACG81A_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014506.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5654..5910)
                     /locus_tag="ACG81A_001127"
                     /pseudo
     CDS             complement(5654..5910)
                     /locus_tag="ACG81A_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6097..7008)
                     /locus_tag="ACG81A_001128"
     CDS             complement(6097..7008)
                     /locus_tag="ACG81A_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5014507.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7027..8031)
                     /locus_tag="ACG81A_001129"
     CDS             complement(7027..8031)
                     /locus_tag="ACG81A_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFS5014508.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8028..10151)
                     /locus_tag="ACG81A_001130"
     CDS             complement(8028..10151)
                     /locus_tag="ACG81A_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS5014509.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10156..12603)
                     /locus_tag="ACG81A_001131"
     CDS             complement(10156..12603)
                     /locus_tag="ACG81A_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5014510.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12590..12979)
                     /locus_tag="ACG81A_001132"
     CDS             complement(12590..12979)
                     /locus_tag="ACG81A_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5014511.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13039..13542)
                     /locus_tag="ACG81A_001133"
     CDS             complement(13039..13542)
                     /locus_tag="ACG81A_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS5014512.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
BASE COUNT         4221 a         2710 c         2509 g         4291 t
ORIGIN      
        1 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
       61 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
      301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
      361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
      421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
      481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
      541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
      601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
      661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
      721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
      781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
      841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
      901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
      961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     1021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     1081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     1141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     1201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     1261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     1321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     1381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     1441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     1501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     1561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     1621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     1681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     1741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     1801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     1861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     1921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     1981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     2041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     2101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     2161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     2221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     2281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     2341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     2401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     2461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     2521 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     2581 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgatattta
     2641 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     2701 ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa atttgcttac
     2761 gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt
     2821 gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca aataaagcat
     2881 ctccaactta catggttaca tgaggatatc aaatcgaaat tactcaagct tcctatggct
     2941 agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc
     3001 attagaaagg aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc
     3061 gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta
     3121 ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg
     3181 aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa
     3241 ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg
     3301 gatcgctgtt gtgtgggttc aatcattcgc taggtatttt attgttcgca cgggtggtac
     3361 aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc
     3421 ccatacatga acgtggtcgg gcattgggag caataggtgc gtttgtttca ttaggctcga
     3481 ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt
     3541 ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag
     3601 atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga
     3661 ttatcagctt ttttggagca atctttttgg ggcaagaaag tggctttttc catccagtcc
     3721 cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga
     3781 aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt accatgagct
     3841 tgattacagc cttgttgatt ttttcttcca acttctttgt caatgtagtg attccttttt
     3901 atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg gctgttgatg atggtctttc
     3961 ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg gttggcccgg
     4021 agattttggt attagtaggc ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt
     4081 tgtcagtgga ctctcctttg tggctataca ttgtcgctac tgccatcatg ggtgccggca
     4141 atgccttgtt ccagtccccc aataatacga tggttatgag ctcggtcact atcgaaaact
     4201 tgggactggc aggctcgatg aattcctttg cccgaaattt ggggatggtc agcgggattt
     4261 cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccag caagtaacta
     4321 actatatcgc aaatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca
     4381 gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag
     4441 gtattcagaa aaatgtctga aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg
     4501 gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc
     4561 aagtattatc ttcaaaatac tagtgctcaa tgagatgctt gatttgatca caagaagact
     4621 gcgaaagccg ctggcaaccg caagctaggt atcccaccga tggactacgt tttagaggct
     4681 actggttatg aatacggagc caacgctctc atctgcataa gaatacatca cccagattat
     4741 gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat agataaatga tttccgtcta
     4801 gtcaaattgg ttcagaatgt agacacacag atggtaagaa gtcactcaca attcgagaat
     4861 ggacttgtcc tatttgccgt atacatcata atcgaaacgt caacgatagt atcaatattt
     4921 tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca tcaagaacct taggaacaac
     4981 ggggatagct tggttaataa gagataccgc tgtttaggct tgcaagtata ctcttttatc
     5041 aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc tttagcattt
     5101 ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc tactgaaata
     5161 tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa tcttcattct
     5221 tatgaacgct ggaattgcgg atatgaattt aataaaaaaa acccttaatg attttacttc
     5281 caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg
     5341 tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg aatttgctat
     5401 gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg aattacttgc
     5461 cacttagctg aatttaacga tccttggtgc ttgaaggggg agattcttgg gaatagagca
     5521 tacttgcaag cctatgtctt tactaacaac gatatctctt attaaccaag ctatccccgt
     5581 agttctaagg cttttacttt tatctaagca ataatcgtat tcaaaggaaa tttttgaccc
     5641 ttagaaagtc tcattttatc agacgtgagc ttaggcattg ctgaattttg ttaattcggt
     5701 tttaggttgt gctttgagca taatatgaaa gtattcttta tcacgatttc attcatctaa
     5761 ggtaatgtga tgtgattctg aaattctttc aaaggcagtt tttgcataag ctgacattac
     5821 atcatcaatg acatgtttac aatgtttcac tactaaaata agggggtaac caagaagaaa
     5881 aactgaatga ttatttgtat ctaattccat ggtatatcaa ccatttccta tatagactga
     5941 ttatgacatg gattaaaata aaaaggcact atgtcttaat tgtcggtttt cgagttaccg
     6001 aatggcattc atcacccacc tatagaggat gggcgacttc tgcctgaaat aagttacact
     6061 acttataact tctaataaaa ttagattgag tcattattat ttaacatcaa cgattttcca
     6121 gtttccattt ttttgtaata ctaatttgta ctgaaagtag ctagtggttt ttgatacatt
     6181 atcaaggtat tttatctttg tactcactag aaaatttttt ccgttttgta gataaactgg
     6241 attctctaac tccgaaaact ttaagtcaat attgattggt cgcatcgcac cattttcaac
     6301 ataatactcc aattcttttt cagaagctga tggataaagt ttaaaaaatg tttctaaaaa
     6361 atcggtgatt tcttccgttg ttttggcatc aatttctgaa tcgttttcta cttgttttgc
     6421 ttcatatttt gccttgtctg gctttccaat cattgttggc aaactagtta ctaaatgatt
     6481 cctgttttca tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt
     6541 tccctctgta atatgttgct ccactaaaaa tgaaacgaca aattcttccg agtgtttttc
     6601 aacatctaga atttttacag attgaacttc ggaactcgtg ggaatatctg ctcgaaccat
     6661 atcttggatt aaagcgagac cgttttctgt aaggtactgt tcgagattac tcattctctt
     6721 ttcaattgct tctttgttat ttttccatga aaaatattct ttcgcaaaat cctctgtaaa
     6781 actttcaacg ccagaagtat tcactacctt ctcttttata accacttttt catgagttgt
     6841 atgttgatca atggcggtga aatttttata aacgccaaaa gcaagactac aagacaataa
     6901 aagccaaagg gcaagcacca tttttcgatg cttacccacg gatacaactc gttctttttt
     6961 cttttttggg ctcttttcct tttgattccg ttcaattttt atcttcattc tttttcctct
     7021 tttctcttat ttgtatcggc cagctccgat aagatgctgc cgccaatagt tttccgttag
     7081 attcgtccac cccactggat ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc
     7141 cacatgggtc acataagtac cggcatcata ggttgaatga aagaaaacta aatcacctgg
     7201 tttggcatct tttagatcaa tatgagtcat ggcatcatat tgatcctgag ccacccgagg
     7261 aagtttcagg tcaactttgg cataacacca ttgggtcaag ccactacaat caaaccccgt
     7321 ttctggactt gagccaccaa acacataggc cgttccttca tacttaaatg cctcatcaaa
     7381 aatttcttgg actattttgt catcaaactg agttgtagtt aggtattgct tcactaataa
     7441 acagtaaaac atattgccat aattataccg ccacccacca ttttccttaa tcgcgattgg
     7501 gttggggtaa tcgacttttt gaccacctga tttttccttt gaaaaagatt cagctaaagc
     7561 aaaactatgc ttcttcccgt ttttcccgac atgatctaga tacccgccac catagttata
     7621 ggcctgaata atactatctt gatcacaccc tagttctttg gaacgcttaa ccaaagacgc
     7681 aaaatagaga cagccttgtt tgatggatgc ctcggtactt aatgaattag gtggtaatcc
     7741 ggctgactca gaactttgca taacgtcttc cattgtgcca ccagattcca cttgaataat
     7801 cgccaaaaga attggaatat actctgttat atccgattct ttggcatact tttccaccat
     7861 tgcacggtgt ttcaatactt cttctgagac actagttccg ccaggagaag tgccagaact
     7921 accatttccc tcactgtctt caccaaacaa caaacccaca cacatcaaaa tacccataaa
     7981 agagatggtc agaaaagtta atgaaataac acccactttc ttcaagttca ttttttctca
     8041 cctgaccttg attttggacg atggttcatt cttttttgta ctttttgatt ggtctgcttt
     8101 ggcagagtgg acctctgttt cacttgttgg aaagaatttt tcttacttgc tgattggctt
     8161 ttcccttttg aaatgatttc ttgactagtt agttttcgtt tgatttcggg attcagattt
     8221 gctctcacaa tccgctttgg tgcttctttt ttcagcatca tatctttctg aggttgagct
     8281 gctttatcta ttggaatatt cgcccttctt tttccctgac gatttgttgc ttcactcagt
     8341 atcttgcgtt tttcgtccat tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc
     8401 actcgatccg ctttttcttg gaacagtccg cgcttgaatt cttctggtgc ttttttcgac
     8461 ttttcgattc ctttatggag attgtatttc aaattcgttg gcgtattcct tacctgatcc
     8521 tttacttgtt tagtcttatc tttcaccaaa gcttgggtat ccaaaacttt gccgattttt
     8581 ctcccagcca aattcattcg ggattgtttc ttattatttg aaactggatt ctccttagtt
     8641 aagtcagata gtatttcgtg atcgttcggt aatcgtgaat tctttcgtag agaagaaccg
     8701 gatggcttaa atttggattt tgattgttcc tttgccaact tatttccgac tgctgcacca
     8761 ccaccaaaag ccaaagctcg tcccacattt cgattgagtt gccgcaattt tcgattcatt
     8821 aacatttgcg gttttcgcat gacgcgtctt cccattgact ggctatcatt actctggagg
     8881 ttgaacatac tcataatgtc gcccaatttg aaatagatac cggcaaaggt cacaatttgc
     8941 agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat cgtggagatg
     9001 ctaaatgcag tcgtaatcac caaagtaaca cccgctcgca tcataatcgt attgaataaa
     9061 cgaataatcg ccttctttcc taaactttca taagtcggca acatggacaa taagaaacta
     9121 attggcagaa acatcgcaaa gataatgaag agaatttgcg agaacaacat aatccctgtt
     9181 agaagaaaaa caaagaagga aatgccaata ttgaacaata ccaaaaagac cacggtccct
     9241 aaacggttca tggtcttggt aatactgaga ttcgcattgt cattgtcttc aatctctgcc
     9301 ttgaccgctt cttcccgatc tttgcctttg ttttcatctg gactcacaga tagaatcttg
     9361 ttgacccggt cttcaccaat ttcttctatg ttggaatcat caaattgcag tagtaaccaa
     9421 ggttgttgga cttgaatcga aaacaaacta tcccgaatca aatccacact atcttttcct
     9481 tggctttctg aattgggtac aacgatctcg gttccaaggg taagggccgc ttcactaaca
     9541 tctgagctaa agtcattgat ttttgtgata taggtaggcg cataagcaat gaacgaaccc
     9601 gatagtagaa aaatcatcac aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt
     9661 tttaataatc cggtatacgc cacatacacc cccagagcta aaatcatcaa taatagaaac
     9721 ccaaaataaa atccggaggt ctggagtcca ttttcagtaa taccagccaa tgtttggata
     9781 ttctttccaa tactttcagc cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc
     9841 acataacccg tggcgttcga caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca
     9901 taaagaccat acatcacgct tttccctaag ccatcacccc aattccaagg caaccaatcc
     9961 caagagctat ccacaaagaa atctagttga tagttgttca gtgaatattt ggaatataga
    10021 tttcctgctt caactgtatc atcaactagc cctgtcgcct cggctaccgt accaactaaa
    10081 ctcaatagaa ctatcagagt gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt
    10141 ttctttttca tcccctcact ccattctctc ttgaacaggt ggacgggtat caaaggcatg
    10201 gaagagttct tcaaagactg gatgtacctg aaccacgcct acccgaccat acaaatcctg
    10261 catcaagcat tggccatttt ccaaatctcg taggcgtttt tgattgcttt catcttctcg
    10321 atccaagcca aagaattcca aggttttctt gatttcatga atatcggtac tgcgaaacgc
    10381 aaacttcaac ccgatattat tcttcaagcg ttcatccagt aaatcatcac tattttgcgt
    10441 gacaaagtaa accccggcat tcatagcccg cccagcacga acgagcttat tcgataatgt
    10501 tttcccttgc gctacttgaa gaaaactcca cgcctcatct aagtctacaa ttttgaagat
    10561 ttctcgatta ctatggataa agtccaaggc aaaagtggaa atgacgatta gcatggaaac
    10621 actaagcaat tccatggtgg tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa
    10681 atccgctact tgaatgatat ttaactgcct atctaaacta atcgactggg taatcgaacc
    10741 atctgagaaa agcaaatggg caaaatcata atcggtaaag gactcgatgt gatccgcgat
    10801 attttccgca actggtgagc cttcttttcg aagctcatta attacttgta ataatccccg
    10861 ttgattgctt tgggtaaccg tccgaatggc cttacgcaac actggaaact tctccccatc
    10921 tcgactagaa attcctgtta aaaaggtcag aatatcaatt gctagacttt cagaatcctt
    10981 ctttttctcc ataatcacat aaggatcaag taatccttga ttttttggct tactagttaa
    11041 gttgagaata ttgatttcat ccgcaatttc tggcaaggtt tctttccagc caccccgttc
    11101 cccttttgga tcgacaatca cagcttgtcc accaaaaagg acggcgtaat aaactaagag
    11161 attgttacta aaggactttc cgccaccgag tgaaccgagg aatgccgctg ctagcgcatt
    11221 ggtaactgaa tccttgaccc cttgtgaggc caatgccggt ttcaggtaaa cattgcgccc
    11281 cgtatctacg ttataaccaa agtaaatgcc ttccaactcc cctaacatct gagtggcgcc
    11341 aaaaccaagt ccggcgagaa aatcagacgt aacatactgg atataatcat tcatgtatcg
    11401 cttactagaa ggtaagaatt cttcatgtag ccccatcata tcgccaaaag gccggactag
    11461 ttttacattg gtatcatcat aaaaatctag cacttcatca caccggcgct ttagttcatc
    11521 tactgattcg gcacttactc gaaccacata gctcagtttg tacatggatt ctttggactg
    11581 atccaaggta gtttccaatt catctacgga atccaatgca tctaagacat tggaatttgt
    11641 ttcattgtca gattgatagg catggttgtc caagtctttt aattcctttt tcttattgcg
    11701 aacggtagca agtgcctttt tgttcgtcac aatctccaca ttcatactag tgtcaattgg
    11761 gaatgtaaat tgttgttgct ggtaatagaa aagttctgag gagggaaact ccatttcccc
    11821 cacaattgta ttaattgtta agtacgctac atatgattca tgattctcat gttccatgca
    11881 taagtaccgg ggcttttctt caatcaaaca acgactggga cgtaataagt cgtaacgttt
    11941 caccaaagtt tccgacttca gctttttctt tggtagctga aattcatatt cttcaaatgg
    12001 tgtacccttt tcgccataga tatgttcaat caaatacatc agatcactcg gtgtgactcg
    12061 tctgacttta aatcttcgag ccaatttact ttccattagt ttttcaagct tggtatatcg
    12121 gcgaatttct tcattggata aggaaacaaa atcgcccatc aaatgatgat tcactccata
    12181 gacaaattct tgaaagccgg agaagaaaga tttcttgaga ttctttagat tgacctcttc
    12241 atctgtcgca atcagtttga agccaataaa aaagcgataa tcaatctgac tatcgccaat
    12301 catggatact aatgcctctg tctgtaaatc aattcgttgt ttcgctactt ctgttaatcg
    12361 cccggtaatt tccttttttg atcgttcttg cgtggcacga atactacttt ctgtggcaat
    12421 ttgtaaggca tgaatcttgc cttcacgatt ttgcgcaatc aattgacgaa agttatcgtg
    12481 gacttgatat ttctgttcag gtgacaaaaa ggagtaattg taaggtatta actcatagta
    12541 ggcaaaacat tctccctctt gattaaagac aagattattt tcaatatact taatcgggta
    12601 catagttcac actccttacc agagtaatgg gttcgtccca tcgtttcttc tgttctttga
    12661 ctttctttcc tgcgaaagtg accttaaatc gtaagtgata gctaataaaa gatcgtaaaa
    12721 atcccaatgg tttcttaccg tcaaacgttt tttgcgacat gaaccaagta actgccaccg
    12781 gtatacctaa gtattttaaa aaggccccct ctatcataga tagtggaggc acatgggcaa
    12841 atacgatgac aaacagcaag gataaaacaa accaagccat ctggttaaaa gtcacaggaa
    12901 aaggcagttg aaagtcatta attgcataaa tgaccttttc aactgcccaa atactggtgt
    12961 agctttttat tttcttcatc aaatcattcc tttaaaaaga aggatagttt agaaatcaac
    13021 tacccttcaa taattttgtt aatatggata ctcaaaaaca cctcgactcg ttaccaaata
    13081 attgcctcca atctccaagt ctctaccaca cgcatcaaaa tcaatatacg tcttcaagtg
    13141 ttctggtact tctccaaaat caccagcttc tacaatcaag aaatatgcta catcactcat
    13201 atcatcgcaa tctggataat agataatatc atccacatgc tctaccatct cctcaaaaga
    13261 attgaagaaa gcatgttgaa tctcagaagc aacttcacca actggcgttc cttcaagttc
    13321 ctccgctaag ccacacaacc gattaatttc ctcaattggc gtatactcat caatctcaaa
    13381 gggaagttca taatcatgaa ttgcatattc ctcgtattcg tcatttaacc caattcgttc
    13441 tttcacttca tcaaaatcga caggtggggt aaaccacgcc cccaccaatt caccttcgtt
    13501 atatttcccg aggttcgcaa tataaacacg catctgttcc atcgtgcttc ccccttacgc
    13561 accgacaatt cggttaaaca aactgagtaa gacatccttc accccagaag cattaaagac
    13621 caatccgacc gcaatcaaag caatcaccaa aaagccaatc agtttggaaa actcccgctt
    13681 aaatcctaaa aacaaaccaa tgaccacaat tgccagtaag actaaggatt g
//
LOCUS       DAZULY010000060        13598 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000060 DAZULY010000000
VERSION     DAZULY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.061"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            139..477
                     /locus_tag="ACG81A_001134"
     CDS             139..477
                     /locus_tag="ACG81A_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014513.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(682..1257)
                     /locus_tag="ACG81A_001135"
     CDS             complement(682..1257)
                     /locus_tag="ACG81A_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HFS5014514.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1989..2570)
                     /locus_tag="ACG81A_001136"
     CDS             complement(1989..2570)
                     /locus_tag="ACG81A_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5014515.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2753..3379)
                     /locus_tag="ACG81A_001137"
     CDS             complement(2753..3379)
                     /locus_tag="ACG81A_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFS5014516.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3401..4732)
                     /locus_tag="ACG81A_001138"
     CDS             complement(3401..4732)
                     /locus_tag="ACG81A_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFS5014517.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4887..5288)
                     /locus_tag="ACG81A_001139"
     CDS             complement(4887..5288)
                     /locus_tag="ACG81A_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS5014518.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5374..5580)
                     /locus_tag="ACG81A_001140"
     CDS             complement(5374..5580)
                     /locus_tag="ACG81A_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014519.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5675..7177)
                     /locus_tag="ACG81A_001141"
     CDS             complement(5675..7177)
                     /locus_tag="ACG81A_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFS5014520.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7195..7650)
                     /gene="argR"
                     /locus_tag="ACG81A_001142"
     CDS             complement(7195..7650)
                     /gene="argR"
                     /locus_tag="ACG81A_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS5014521.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7748..8365)
                     /locus_tag="ACG81A_001143"
     CDS             complement(7748..8365)
                     /locus_tag="ACG81A_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS5014522.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8586..9959
                     /locus_tag="ACG81A_001144"
     CDS             8586..9959
                     /locus_tag="ACG81A_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5014523.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10034..11146
                     /locus_tag="ACG81A_001145"
     CDS             10034..11146
                     /locus_tag="ACG81A_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5014524.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11218..12855)
                     /locus_tag="ACG81A_001146"
     CDS             complement(11218..12855)
                     /locus_tag="ACG81A_001146"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFS5014525.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13023..>13598)
                     /locus_tag="ACG81A_001147"
     CDS             complement(13023..>13598)
                     /locus_tag="ACG81A_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HFS5014526.1"
                     /translation="TFAAGATAMFVGTTNAHADEVYTVKSGDSLSKISQKFAGDNSMI
                     DAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTETATQEHTYVAPV
                     ETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQLDASYLNGDYSA
                     ANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3665 a         2868 c         2447 g         4618 t
ORIGIN      
        1 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
       61 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
      121 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
      181 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
      241 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
      301 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
      361 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
      421 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
      481 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
      541 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
      601 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
      661 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
      721 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
      781 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
      841 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
      901 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat aaaagccact
      961 cattgggaaa acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac
     1021 tgtctctgca cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt
     1081 catgatttcc ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga
     1141 cggaaaaact tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc
     1201 tcgataataa ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct
     1261 aatccctccc aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac
     1321 gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg
     1381 tattctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct
     1441 ttttgaaatt gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac
     1501 ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca
     1561 ttttcacaaa atttttctta atactcgaaa ctaagcgact ttttcacaac ctcttcctta
     1621 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagca
     1681 gctattttta tttggctgtt cttattactt gatgcagggc agctttttca caacctctcc
     1741 actaaacggt gttcaatcag ctaaccttcg gtattagctc aaaaaacggc aaaatatgag
     1801 gaactatttt gccgtttgtt tctctaatac tcaggtcaaa gcgctgattt cacaacctct
     1861 aatacacggt gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaattt
     1921 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
     1981 tctaattatt aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc
     2041 tgcctaagta gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca
     2101 gcaatcaacg ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt
     2161 gccaggatat tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga
     2221 cggtattctt catccactgt actcaactca ataatcaaat tacctacagg acagccatga
     2281 taagtgatct gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct
     2341 tctggctgtt ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata
     2401 tgcgcatgga tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga
     2461 ccttttcctg tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt
     2521 ttataaataa gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc
     2581 ctttttcatt tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc
     2641 gtttaaaaaa gactgtgcca aattatctga cacagccctc actataaaac tgttctcata
     2701 tagctagatg ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata
     2761 gttgctctaa tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca
     2821 acgctgcatc cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat
     2881 tgaatccact ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc
     2941 caccaagaat tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt
     3001 ctggtccaaa gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca
     3061 attcttcgat aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag
     3121 caaacatctg ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt
     3181 catgtgggta aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt
     3241 ttaccatttc aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac
     3301 caaagccacc gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg
     3361 tatttttatt attgaacatg gtggattctc cattcgttat ttattttaat acatcattta
     3421 gagcttcact cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt
     3481 ttccattcat agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac
     3541 ttgcccctag aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat
     3601 cttctaatac ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga
     3661 cgccttgtgc tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa
     3721 atacactgta aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact
     3781 gatccataat gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga
     3841 cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg
     3901 ctccgcgatc tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt
     3961 ttcttccagt tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg
     4021 taaaggtgac ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat
     4081 ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt
     4141 cacgtggaag aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga
     4201 acattgatgc aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta
     4261 attgttcctg atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt
     4321 tgattggtaa aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa
     4381 tcactttttc accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac
     4441 cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca
     4501 tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat
     4561 tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac
     4621 gtgttgactt ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag
     4681 tcttaccgcc ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct
     4741 cgcttctttt ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc
     4801 caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt
     4861 agtctggttc gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct
     4921 ctagttgctt tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct
     4981 tcttcgctga tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca
     5041 tctaaattat aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct
     5101 ttgatcatat gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt
     5161 tctggatttg ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca
     5221 tttggtgcct cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct
     5281 tttttcattg tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta
     5341 tgacaagtaa ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc
     5401 tactattttt ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc
     5461 aaaataaata gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata
     5521 taccagaaag ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat
     5581 tttttcctcc ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg
     5641 ccgtccaagc aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat
     5701 cagaaaaatg tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt
     5761 catcattttc tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc
     5821 agccgtttcc gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga
     5881 agttcatccc tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt
     5941 gttcgcgtgg ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt
     6001 tcaactcttt gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca
     6061 tcaggatctt tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag
     6121 cgtgtccgct tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg
     6181 ctttattgat tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt
     6241 caatgaatct ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat
     6301 gctcttttaa tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc
     6361 cagaagcact gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg
     6421 cttcatttgc taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg
     6481 ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac
     6541 ctaagctatt caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa
     6601 tgattttcca accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg
     6661 tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt
     6721 cgctttttat tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc
     6781 cttctgaggt aaagaatttt attccattat aataataggg aagatggcta gcagtcagca
     6841 taaccgcagc atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag
     6901 tagctaattc gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct
     6961 ctttcagtgc tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat
     7021 ttggatcggt tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt
     7081 ttaccatgtc tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa
     7141 tatccgatcc attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc
     7201 cctcattttt tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat
     7261 aatgaatgta tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag
     7321 ccaattcgtt accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac
     7381 agactggata cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga
     7441 aacagatggc tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac
     7501 taacttcatt tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc
     7561 atgcagccgc gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg
     7621 gatcagtgct tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca
     7681 aaagataagt aatatttctt catattttgg tattttttac ttttattttt tgttttcatt
     7741 caggttgcta acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg
     7801 attttcttgg atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt
     7861 cttttgcgat gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga
     7921 cgtgtaagat ttgggcacaa agaccttctt ccatcttttt gaatactagt tgttctttca
     7981 agtcttccgg aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa
     8041 agtcaggctg tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat
     8101 gcaaatactt ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat
     8161 aatcaggaat cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata
     8221 gacaggctac tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa
     8281 agaatggttt tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt
     8341 ctttacgcca ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta
     8401 ttatcatacc tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg
     8461 ctagaataag tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag
     8521 gcggtttcta tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga
     8581 tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga
     8641 tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca
     8701 gacagtttga tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga
     8761 tttcgatcat tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct
     8821 ttttagcttc aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct
     8881 tcatggtcct attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt
     8941 tgatgttcca tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg
     9001 tcggtacttt gacaacggcc attgctccag caattggccc cacttatgga ggcattatga
     9061 cttcacattt tacatggaat catatcttcc tgtttcttgt gcccgttttg attctttctt
     9121 tgattatcgg ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt
     9181 ggataagcgt tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt
     9241 tgggaacagt ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt
     9301 actatcgaag taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa
     9361 cattccgctt gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt
     9421 ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag
     9481 cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg
     9541 atcaatatgg tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc
     9601 tagcattatt cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt
     9661 tttatatgat cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac
     9721 tgaatgaaga actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg
     9781 gcgctgtggc tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc
     9841 ataactatga agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt
     9901 tgcttctcgt aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga
     9961 ttccatatac ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa
    10021 aggaggcttt tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgactct
    10081 agccagttac ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca
    10141 ggcaactaaa gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca
    10201 atggtatcgt ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca
    10261 acttgatcaa acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt
    10321 agctgaattg gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat
    10381 cgaatggctt tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc
    10441 actgcgtatc agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca
    10501 gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag
    10561 cgattcttgg ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac
    10621 acctggcccg gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc
    10681 gcaaaaaggg cagctcttag catttcagac atcttataag atgagtcaag actggcctgt
    10741 tgctatgctt gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg
    10801 tgctacccat gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg
    10861 attgactaag agtacagaac agttcttgac tgatccagat cagataaaaa actggccata
    10921 cgagttccgt gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact
    10981 gccagatgat ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg
    11041 tccttatatc ggctatctat tagccaactt cttaaatact ggtcattggt ctacggaaga
    11101 ttatcaaaag gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt
    11161 caagtaagga tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta
    11221 gttcaatttc tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt
    11281 tcctatctga gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt
    11341 ataggacgta tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc
    11401 aaatggacca aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa
    11461 tgtcgtttca ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc
    11521 tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac
    11581 acggaaagga ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat
    11641 attttccgcg tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg
    11701 gtcattgatc ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc
    11761 gatcttttcg ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg
    11821 cccatttccg tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt
    11881 cacgcccaca ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc
    11941 ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat
    12001 aacgaaagca tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt
    12061 aatcgttgat tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt
    12121 actagcatat tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc
    12181 ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc
    12241 cgctgtatta tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca
    12301 ttcgggatca caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt
    12361 ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc
    12421 aaatccttct ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat
    12481 tacttttgta tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt
    12541 ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct
    12601 tcccacaatg cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc
    12661 ttcagcatgt gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg
    12721 ataagctgtc ttcctgtcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac
    12781 aatcgatcga atactggata acagtggatt tccacttgtg atttcgttta attcgagact
    12841 ctcttttgta ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata
    12901 cattattcga gtttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac
    12961 acaaaaaaac cgagaacttg aataaacaag tttctcggtt tcatcatttt ccttttatcg
    13021 gcttagtacc agccgtttgc taaccagaat gttttagcag catcccatga accataacga
    13081 gatgttacat attgttctgc tacacgttct tgattggcag ctgaatagtc gccgtttaag
    13141 tatgaagcat ctaattgata acggccgatg taacgtccgt tagtagctgt gtatgaaccg
    13201 cctgattctt tttgtgcgat ccattctttt gcagagcttg aagttggcgc tgttgcagca
    13261 gctggggcag caggagctac ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc
    13321 gttgctgttt cagttgtaga tgctgtattt tctgttgttt tgttttctgt tgttgctgaa
    13381 gaatcatttg aagttgggat tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg
    13441 atcgaatttt tttcagcaat tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag
    13501 atcttagata atgaatcacc tgattttact gtataaactt catcagcgtg agcatttgta
    13561 gttcctacaa acattgcagt agcaccagca gcaaaagt
//
LOCUS       DAZULY010000061        13577 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000061 DAZULY010000000
VERSION     DAZULY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.062"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1046)
                     /locus_tag="ACG81A_001148"
     CDS             complement(99..1046)
                     /locus_tag="ACG81A_001148"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFS5014527.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1236..1505
                     /gene="rpsN"
                     /locus_tag="ACG81A_001149"
     CDS             1236..1505
                     /gene="rpsN"
                     /locus_tag="ACG81A_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS5014528.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1681..2043)
                     /locus_tag="ACG81A_001150"
     CDS             complement(1681..2043)
                     /locus_tag="ACG81A_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5014529.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2054..3175)
                     /gene="alr"
                     /locus_tag="ACG81A_001151"
     CDS             complement(2054..3175)
                     /gene="alr"
                     /locus_tag="ACG81A_001151"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFS5014530.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3191..3541)
                     /gene="acpS"
                     /locus_tag="ACG81A_001152"
     CDS             complement(3191..3541)
                     /gene="acpS"
                     /locus_tag="ACG81A_001152"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFS5014531.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3671..5182)
                     /locus_tag="ACG81A_001153"
     CDS             complement(3671..5182)
                     /locus_tag="ACG81A_001153"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS5014532.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5504..6856)
                     /gene="murF"
                     /locus_tag="ACG81A_001154"
     CDS             complement(5504..6856)
                     /gene="murF"
                     /locus_tag="ACG81A_001154"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFS5014533.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(7009..8085)
                     /locus_tag="ACG81A_001155"
     CDS             complement(7009..8085)
                     /locus_tag="ACG81A_001155"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFS5014534.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            8281..10026
                     /locus_tag="ACG81A_001156"
     CDS             8281..10026
                     /locus_tag="ACG81A_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFS5014535.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10306..10743)
                     /locus_tag="ACG81A_001157"
     CDS             complement(10306..10743)
                     /locus_tag="ACG81A_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFS5014536.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(10727..12100)
                     /locus_tag="ACG81A_001158"
     CDS             complement(10727..12100)
                     /locus_tag="ACG81A_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014537.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12422..12910)
                     /locus_tag="ACG81A_001159"
     CDS             complement(12422..12910)
                     /locus_tag="ACG81A_001159"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5014538.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(12932..13534)
                     /locus_tag="ACG81A_001160"
     CDS             complement(12932..13534)
                     /locus_tag="ACG81A_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS5014539.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3887 a         2945 c         2458 g         4287 t
ORIGIN      
        1 tttatctatt atacgtatac gcaaaaaagc taaaacattt tttgtctttg cccctacaaa
       61 aatgtttcag cttttttcgt tttttattcc tgtatttctt atttttcgaa tgacaggatg
      121 attttcccag caggaggatg tccttcaact agctgatgtg cttggatgac atgcttaagt
      181 gaagcagaca gattcattga aataaaaaca tgaaaggctc ctttttggta ggctttgatt
      241 atgccagcaa aggcttctgc atctagatat tcctttcttg gaacaaacga ttcataaaag
      301 ccttcctttt tttgccgcaa atctagatct gggaggtcat ttaatgcaac atacctgccg
      361 cctggtttca tgatctggat gccggtttct cctttgatac ttcctttcgt cgcatcgatt
      421 acaaggtctg cctgatcagc aaattgcaga cctggatttg ttttatcata agcagcaaat
      481 gctgaagctc ctaatttttt taccttttcc tcatttttac tggatgcact ggttaagatt
      541 cggattccct tttcatggag aagctggatc agacttgacc cgaccgcacc tgatgctcct
      601 agtatcatta cagtatctgt cggctggatt tccgtcaaat ggttgattag attataagcc
      661 gtaattcctg gcgtgaccat tccggctgct tcttcccaac tcattttatc tggaattttt
      721 gctacttttg ctgatggcaa cacgacttct tctccgtaag tcccgccgac tgcatggaca
      781 gctactcgat cacctacatg aacagttgtg acatctgaac caatctctgt cacaatacca
      841 gcgccatcat tacctggtac ataagggaat ttcaatgttc gtatctcttt catttctcct
      901 aaacgcaagg caacatcata aggattcaca ctgaatgctt ttatttctac acgaacatgt
      961 ccagaaccga cttctctggg atgagcatcg atttcttcaa aaacatcttg tgcttctccg
     1021 taaccgttaa ttgcaaatct tcgcatggtc accatctccc tttttcttta agtttacgtt
     1081 tatgccttta ttttgacaaa aaaaatgtct tttcgttttg gcgcatgatt caaaacaaaa
     1141 tagttgtctt ttttcgcgta acgtgttaaa ttatttatct aaaagcgtaa agattacgat
     1201 ttacgctaat aaaaaataat tggaggaatt tgttgatggc taaaaaatca aagattgcca
     1261 aagcaaaaaa acagcaagaa acgattgaac gctatgctgc tttacgccag gaattaaaag
     1321 cgcaaaaaga ttacgaaggc ttgcgtaaac ttcctttaga tgctcgtcca gaacgattga
     1381 aaaatcgtga tttaatcgat ggacgccctc gcggatacat gcgtaaattc gggatgtccc
     1441 gtataaaatt ccgtgaatta gcacataaag gtttgattcc aggagtaaaa aaagccagct
     1501 ggtaatccaa gtctggctgt aatgtaaagg aggagggcgc atggaccact ccttggatca
     1561 tcaaaaaaga tacgttcgct tcttctagca gaaatgctgg tactagaagc agacgtatct
     1621 ttttttacgc acagctggtc cttttttata tgtaaaggct gcatggttac ttcgctatat
     1681 ttatttagta acaggagtca gtgcttcgat acccacacta attttcaatg ctcggtcaac
     1741 ttctgccata atgggtcgat ccaaatggca gaccttttct ttcaatcttg atttatcgat
     1801 cgttctgatc tgctccaaca ggataacgga gtctctttct attcctgttc cagtagcttt
     1861 gatcccaatg tgagtcggaa gcttaggttt agccattttt gccgttatgg ccgcaacaat
     1921 gatcgtcggg ctaaaatggt tacctagatt attttgaata accaaaactg ggcgtgttcc
     1981 gccttgctcc gatcccacga ctggagaaag atcagcaaaa taaatatctc ctctttttac
     2041 cataggatcc tccttattct tgatattctc ttggtatccg ctgtcctaat ccgcaagcca
     2101 cttcatagtg gatcgtacct aattgatctg ctatatcctg catagtgatt tctttgtctg
     2161 catttttgcc gataagcgtc acttttgttc cgacaggaaa ttcttgaggc aatcggatca
     2221 tcagttgatc catacataca cgcccaatcg tttcacagta gttcccttct accaatagag
     2281 aaaaaccctg catctttcgt ggccagccat ctgcataacc gatcggtatc gttccgatcc
     2341 actctgcttc aggagtgata tatgtttctc catagccgat tccttcacct gctgggagtt
     2401 ttttgacttg aatcaattca gaaaccagct caagtgctgg ctgcaatgga taaacttccg
     2461 gtagtgcgtg cccagaggga ttaagtccgt acattgctac tccataacga atcatattgc
     2521 cgattgtttc atcatgccat aaagccgttg cactattgct gacatgtaca tatcgaggaa
     2581 gttcgggaag ttttttcagc acttctataa agcgttcttt ttgcagattc caatatgtat
     2641 catctgcctg atctgctgta gaaaaatggg taaataaacc ttcccattct aatacacgac
     2701 tttctttgat gaatgcagct gcctctttta cttcttcagg tgtacaaaat ccgatacgac
     2761 ccatcccagt gtctgctttt agatgaatcg aaagaggcgt ctctagctta cttttctcta
     2821 aaacagcagc tgctgcttgc aaccattccc gtgtacctgc agttactgat aaatcatgag
     2881 caacagctac agtcagtgcg tcgtaaggaa ccatattcaa gataaggatg ggctgagtaa
     2941 atccagcttc tctcaactcg actccttcat ctaaatttga aacacaaaag cccgaagctc
     3001 cacctgcaac tgctgcctca gctgtttcaa tagctccatg accgtatccg ttcgctttta
     3061 ctactgcaaa caattctttt ccttgcggaa gtcttgcaac ttcatttgct acattttcag
     3121 ttatagcttt tttatgtatc actgctttcg ttgggcggtg ccaagcgaca accatcgttt
     3181 ttccttcttt ctagtcttcc aaaataattt gtgcaaaagc tgtttcattt gtatgagtaa
     3241 tactgaccca tacattgcct tcatgaggtg aacgcgtcac aacaggctga ccattttcat
     3301 tttttaaaat ctcgacatct tgaaaggtaa cttttccgat cccagttccc caagctttag
     3361 agaaagcctc tttgcaagcg taacgcccgc ctaaaaattc tacttggcga tgaaaaggca
     3421 aggactggaa tagtttcatt tcatcagagg tcaagattcg tgcaatgaat tttggttttt
     3481 cttcgatcag ccgagtgatc ctaggaagtt cgactgcatc gatcccaatt ccttttatca
     3541 tatgcttttc tcttttcaat aaatttagaa cactctcatt ctaccaaatt ataaccattt
     3601 tgccacaaat aagtccacaa ataacaaaaa tgaaagtttc tcttaaaaaa gagaaacttt
     3661 cattttatgt ttaatcgtta tttgaacgga ttacaaatcc gcgtttttta tcattgtgac
     3721 gtttaccgcc atctttttgg ctgttattat ttttgctata accgcctttt gatttgtttc
     3781 cacgataatt accgccgtca cgacgattac ggttgtttcc gccaccacgt ttgccatttt
     3841 tattgtatcc ttttttctgc attggcaatg ggcgttctgg tgtgatcttc actggcactg
     3901 catcagctgg atcttttgct gtcgttttta gcaaaagtgc aactaaatct tgtgcagaat
     3961 attcttctaa taatttatct gctgtttgca ggtatttatc tagaccattt tctgcaagtt
     4021 ttgtttcgat ttgttcaact gcagcaccta attgaccttt gaatgcttct ttttcggttg
     4081 gtggacgtaa agtcgtcatc cgttttttcg tcaagttttc aatcacatgc aagtagtcca
     4141 tttcatttgg tgtaacgaaa gtcacagaca taccgccttt tccggcacgg cctgtacgtc
     4201 cgatacggtg aacgtagctt tctggatctt gagggatatc atagttgtac acatgagtga
     4261 cacctgaaat atctaatcca cgtgcagcaa cgtctgtcgc tacaaggata tctaaatgac
     4321 cgctcttaaa tgaacgcaat acgctcattc gtttttgttg agacaaatcg ccatggattc
     4381 cttctgcacg gtaaccgcgt gcttctaatc cgcgtgccaa ttcgtctaca cggcgttttg
     4441 tacgtccgaa gacgattgtt aattcaggtg tttgaacatc aaacaagcga gtcatgatat
     4501 cgaatttttc gtattctttt gcgcggacat agtattgatc gatcaagtca gctgtcattt
     4561 ctttcgcttt gatcttcaca tgatgtggat ttttcatgaa tttaacgcca atattcttga
     4621 tagctggcgg catcgttgct gagaaaagta atgtttgtct ttgatcagga acttgtgaaa
     4681 tgattttttc gatatcttcc aagaagccca tattcaacat ttcatctgct tcatccaaaa
     4741 ctaatgtttg aacggttcca agtttcaatg tgtggcggtt aatgtggtca agcatacgtc
     4801 ctggtgtacc cacaacgata tgtggacgat ctttcaagcc acggatctga cggccaatat
     4861 ctgctccgcc gtaaacggct tgtacacgta cttttttgtc gcgccctaaa cgatataatt
     4921 cttcttgtgt ttggatagct aattcacgtg ttggtgcgat aactaaacct tgtaattcgt
     4981 gacgatctgg atcgattttt tctaacattg gtaatccaaa tgcagctgtt ttccctgtac
     5041 ctgtctgtgc ttgtccaata acgtcttttc cagctaatgc tagtggaatc gtttctgctt
     5101 ggataggcgt tgcttcttca aaaccagcgc gttcgactga tttcaacaat tctggtgata
     5161 attctagttc tttaaatttc aaatgaatcc tcctatatgg ttgttaactg aggatgtagc
     5221 ttttttgcta ccccgtcgat ttgttcggtc aaagtgtatt gtttcttttt gacagtggcc
     5281 acgcacgttt ttatccattc ttagcccctg tggcaccacc gaaaaatcac tttgcgaagt
     5341 ttaggcatta gaaaagccaa acgggtcgcg ttcagcttta tcattattat gacatctggc
     5401 ttcaacaagt tgtttctatg gcaaaaccag tccaatttct cttcatcttt cggcaatagg
     5461 ttattctaac atagtttttc gttttctgca agtttacaga tttttatttc atttgctgaa
     5521 gttgttctac cacttcaaat aagcccatgc tgttgctgcc ttttagcata atactatctg
     5581 ttgcttcaag attttgtttg atagcatcaa tcaatttatt cttttctttt tcaaaataga
     5641 acacaggtag ttgggggtat ttttggttca attcttcttt tagtgcatgc atttgttccc
     5701 cataaaggaa aatcatggcg aatttctcag gatcgatatg ttcactcatt tgcgcatgca
     5761 tatttaaaga ctctttccct aattccagca tatctgctaa cacagcgatt ctgcgtcctt
     5821 ttgttggcag atttttaaaa gtatctaata ccagtcccat ggctgttgga tttgcattgt
     5881 agacatcgct caaaatagct gcaccatttc ctgccgttag ccattcggtt ctgttttgag
     5941 tgatagaaac atgggcgagc ccttggcgta tctcaggtat ggacagtcca aagaagcgcc
     6001 cgatcgtata cgcaatcaaa gcattcgtta cattataact tccaggcagc gggatcgtaa
     6061 atgtttctcc ttctactaca aaatcggttt gtgcttttcc ttttgctgtc acttccgcat
     6121 gaacatcgcc atttccaata ccgaatgttg tgactgtttg tactaatgtt gccgttaaag
     6181 gtgtcagcaa cggttcatct gcgggcacaa cgagtaatcc gtctgataca agtccgtcag
     6241 tgatttccat tttggcttta gcgattcctt ctcgcgaccc aagattttct atgtgtgctt
     6301 caccgatcat tgtgattgct gcagcttttg gttgagccat aagtgaaagt tccgtgattt
     6361 cattggcgtg atccatgccc atttccaaaa cgatcttttc tgtatcttgg ggcatgtgca
     6421 aaatcgtgta aggaagacca atattattgt tgtaatttcc ttgcgtctta tatgttttgt
     6481 atttttcaga caagacagac gcggttagat ctttcgtggt ggttttacca ttgcttcctg
     6541 taattgcaac gacttctgcg cctacttttt ccaagtaata ggttgccaat tgctgcatgg
     6601 ctttcaaagg atctgctaca ggaataactg gaaaattttt tggagcttgt tcaacaggca
     6661 agctccaaaa tgaagcagat gctccgtttg tgatcgcttg ctggataaat tcatgtccat
     6721 ctcgcgtacc agcaagagga acgaataaac ttccttcggt tatcttccgg ctgtcaaatt
     6781 cgacattggt aatctttgtc agtgcagagc attcgctgcc cacggcttgt gcaatttctt
     6841 gtattgttag attcattgag atctctccta aaaaaggttg tgaaaaaagc gtttggccta
     6901 aacggcgggt tcgcatgact ctcacttcca agtaaatttc tctattcgta tttagaagct
     6961 agaatgaacg cggttacgca atcactccag cctcttcttt atttctcttt attcattttt
     7021 ttcaaaaaaa gattgacgct gatggtatcg attcattcct aactggatca gttcttcaat
     7081 caaatcaccg tatttcaagc ccatattttc ccataagagt gggtacatac tgaactccgt
     7141 aaatcctggc atagagttta attcattcag gaataattca tttttatttg tcaaaaagaa
     7201 atcgcaccgg ctcaatccgc ttccacctaa catcgtgtaa gctaacttcg cgtactcttg
     7261 cgctttttga taaacttctt ctggcacttc ggctggaatc tgcatttcga ttttattatt
     7321 gatatatttt gcttcataat catagaatgc tacgtctttt acgacttcac caggcaaagt
     7381 cgtccgaacg tcttcatttc ctaatacagc aacttcgatt tcgcgcgctt caattccttg
     7441 ttcaacgatt gctcgagaat catactgata ggctgttgct aaagcatttt gcagctcttc
     7501 tcggttttct gcttttgtaa tgccgacact agaacccata ttcgccggtt tgacaaacat
     7561 cggataaagc aaagaacctt cacattgatc aaatactttt ttaggatttt ctttccattg
     7621 attcttaagt actggtacat aaggaacttg cggcacacca gcagcttgta aaatatactt
     7681 ggtcatgatt ttatccatgg cacatgcact ggtcaatacg cctgcgccga cataaggcat
     7741 attcaatgtc tctaagaagc cttggatcgt tccatcttcc ccgtttggcc catgtaaaac
     7801 tggaaaaacg atggctcctt cttctttgat ttcgcccgga ttgatcactt ttcctgtaaa
     7861 gccttcctct gtctgtccac ttgggtccca tgaaagatgc aagacatctt tgctggcagg
     7921 tttttctgtt agtaatggac ctttgaccca ttgtccttct tttgtaataa atacgagttg
     7981 aacttggtaa taattataat aaatggcatt taaaactgaa aatgcggaaa gaatggacac
     8041 ttcatgctct gcgctgcgtc cgccatatag taaagtaatc ttcaaaagat tgtcctcctg
     8101 tattctaaat cgttcagtga tttactctca tattttagca caaaacaaaa ataaacgaat
     8161 aacttattta taaaaaaacg agccagcttc taaaagaacg agcgtcattt attttttgtc
     8221 agctagatta tttgtcccgt tttttataaa aagttatgat aaagaaaagg agggatgatg
     8281 atggcgcaaa caaatccacg cagccgcttg gcagaaatca ttcgagtgtt catcaattac
     8341 aatgtagtgc caaactttgt acagcaaaaa aatcccgaac aggtaaaaaa agcctttgaa
     8401 gaactaggcc ccaccttcat caagatagga cagatgcttt ctgttcgaga agatttgctt
     8461 tcttctgcct tcactcaaac attcaaaaca ttacaagata gtgtacctag cgacacattt
     8521 tcaacggtca aaaagacgat cgaaacagaa ctttctcttt cattatcaga tatattcgat
     8581 gatttttcaa aatcgccttt tgcctctgct tctatggggc aggctcatcg tgccaagctg
     8641 aagaacggtg acgcggtagt cgttaagatc caacacccta acatcgccga agagatccga
     8701 cttgatcttc aactatttga acgagcgatc cctttgatca aatacattcc tgaaactagc
     8761 gtagttgatc taaaaggggt actccaagaa gtaaaacgtt ccttgatcaa tgaaatggac
     8821 ttcttaaaag aaagtcaaaa tggcgaacaa ttctatcaaa agaacaatgg ctggaaagag
     8881 atccgcagcc ccaagattta tgacgctttt tgttcgaaaa aagtgatcgt gatggaagaa
     8941 atgtctggta aaaacttgaa tcatttaatg aacgccgaaa acaaaactga aacttttatc
     9001 actggaattc aaaacaagca gctgaaacaa gaagttgcaa agcttttagt tgagaacttt
     9061 atgaaacagg tgtttgatga tggttttttc catgctgatc ctcatccagg gaatctgcta
     9121 ttccatgtac taacaaaaga agaacagaca caagctagcc gaaaaactga aacagtccat
     9181 gaaaaagagt tcggttcctt tgcttttcga gcctccactt ctgcagaaga ccctgttgcg
     9241 ccatatacca tcaactacat cgattttggt atgatggggc atctttctgc aggacttagg
     9301 caaaaactca cacaagccgt tttagcgctg tatacgaaag atgcttatcg aatcgaaaaa
     9361 gcggtccttc gactttgtca gcaagaaggc tcgtttgatg aaagtcggtt ccaccaagaa
     9421 ctgacaagtt ttcttgaaca gtattatgat tctcctattg atgaaatcaa cttgcaagaa
     9481 gtattcacac atgtcgtgac gatttgtcat caaaataatc tgcagttcga tcgtgatatc
     9541 acgcttttgc tgaaagcatt tgggacatta gaaggggtga tccgcgtcct cgatcctgaa
     9601 gtttccctca tggaagtcgc ttcgcctttt gctcaacatt attttctcac tcatctggat
     9661 gtagaagata cattgaaaca aagcggatta gacttattgg aagggatgaa ggctgcgcct
     9721 aagatccctc aacagctcca ccatctatta gaaatgtgga cttcgggaca aggaaaagtc
     9781 aatttggaat tgaaaaagca agacaaatta ttgagcagga tcgaaagtat gatcaatcgg
     9841 cttgttttcg gtatgatttt agctgcattg atcgtagggt cctccctgct tgtccaagca
     9901 gcacctgtag aaaacgcaga ggtcgtttcc ttattaggga tatttacgta tgctattgcc
     9961 gcattcgtca ttattttttt ggcaatcgat gcattgatcc aaatgtataa gaagaggaaa
    10021 aagtagaaaa aggttgtgac agaaagaatc agcttcaaga aataagaagg ggttttacat
    10081 aaattgtttc tcaaattttt gtgaaaattc ggcttatttt tcgaagaggc tacttctgaa
    10141 acaccgagaa taaaaaatgt gaaaaagctg ttctgcaccc agtaataaga acaatcaaaa
    10201 agggaacatg gaacacattt atcctgtttc cctgttccct ttttgatgta ttcgagcata
    10261 aataaaatac cttcgttacc tactatcttc tgtacttggt aaaccttagt caattgcctt
    10321 ttttaacgct tgacgaaaag acatatgatc tttctccgta aagggttttg gaccctttgt
    10381 ccttatgcca gcttttctta actttccact atgataacgt tgttctagaa gcgtgccgat
    10441 attcgcttcg tcaaattctt ttccagagtg atggaacact cgcacaccct tcggcaatac
    10501 aagtgaagct aaaccataat cttgcgtgat caaaaagtca ttcttcttaa ccataccgac
    10561 gattttataa tccgcactat cagctccgcg gtctacgtag acgatttgga catgattagc
    10621 gtattctttg gtcgtataat ggtccacact ggtcacgata accacgtcaa gaccatagga
    10681 ttctcctaat tttatgacat cttcttttac tggagaacca tctccatcaa tgaatagcct
    10741 cattcttttt cgccttcttc cataaatgat aaacagttac taatccgctc agtaaaagac
    10801 caacattgat ccattttgcc aagataagca gttgtacact ttctatcggc actgattcat
    10861 ggttcagcgt gatcaataat agatggattg gctctcccat ttcttttgct aaaacaaaca
    10921 aaagcgtcat caataaaaac tggtggatca cactcccttt attttgagta gtatacttga
    10981 caacgatccg gcgatccatt ggaatagctg tatcagtttg ataatattcc ttatttagcc
    11041 agctgcctgt tactacgtgc cagaaactaa gcagaaagac acctatcgca aaaccatttg
    11101 gaatcaagag gaccaataaa gaagaactca gtccaagcaa ttgcatagcc aatggagacc
    11161 atgttaaacg tttcaaaatc catggtccga ctatttttgc tgcaatcaag cctgctgcat
    11221 aaggcaaata cataaaaatc gaaagttttt ctaatccgta cagtatgcca acataaaaac
    11281 tgctaaataa aaaaaggaaa ttccctagca ttccatttag aaaagtaaac agatttagcc
    11341 agcttggcaa cttccattcc ttcttctggt gtcctaaaac aaacacgaac aagatgaaca
    11401 gcagcaagaa accataaata gcaagatcaa aaagcctttc atcgaataac aatcgggcat
    11461 ttctcaaaag aaacaataag ccaaagaata tcaaaaataa aacaagctct cgcgtagcta
    11521 ccagatttct tttgatgtct ttgaaatcca gttcgtaacg cggataatga ctaaccgtat
    11581 ggtaggccat gacgtaaaaa agtgtataaa tagtaaagct tgctgcaatc tgagtcggtg
    11641 caggcaactc aatcgcttga tacaggagaa atagccagat agctgcaaat aagtactttt
    11701 tcccatccat attgataaaa ccttgcgtct tttcgtgaaa attgaccgtg gtattagcag
    11761 gtacaagcca tgctgcactc agccctagca aaacacttga aaaataaaat aaaggaaaat
    11821 aaaatacgcc taatattcca agcaatgaac cagcaccacc aatcagcaga gcgatcatca
    11881 gcaacgtgta actatctaga ccaaatcgta gactgttgac taaaaatagt ccagtcatcc
    11941 gaaaggcata aaacagcgta aatggcaaaa tcgtcagcca attatgcgag tctgttagtc
    12001 ccaaaaataa aagataagga ataaaaataa ccgtgttact taagataaag ggcgttgttt
    12061 gtgtaaaatt acgcccaaat cgtttgagtt tttcgaacat gacaatggct ccttaaacag
    12121 tcttataact aatctgctct tcgattttag cattaaaggg gaaataaacc aattttacag
    12181 aatatattct tgtaaaaaaa aatacagaaa gaagcataag cttctttctg catttttacg
    12241 gtacttgatt actcaagcag acaagccctt ctccacaaaa gtcttgcccg acacccctgc
    12301 atccggcagg tgataaagat agaaatcaat tatcctgtgc ctccaaaatc aattaggagc
    12361 tctgtaaacc agagtccttc tcgacccgtc gcataaattt actttaacac agttcctatg
    12421 tttatgcgag agcttttttc attttataat cgaccatttg aaatccttga ccgatatctg
    12481 aaataagttc ttctgtaatc tcaaatcctt catgcttata aacctcgatt gcttgatgat
    12541 tcccctgatt cactcgcaaa aagatctctc tttcctttgt ttgcgcagca agttcttcat
    12601 accagcgaaa aactgaagaa gtcaatcctt gaccacgtac tttatttagc aaatataatt
    12661 tacttaaata aatttttcca tttatctctt cataagcagt gtaaccgaca gtcttttctt
    12721 cgctttttaa taaaaagtag gacacacctt ctgtcagttc tttttgaatc tgtttttttg
    12781 actgataagt tttcagcatg taatccactt gtttttgacc aatgatcggc acaaaaactt
    12841 ccggccatac ttcttgtatg actgccatca gctcatccag ttcattttct gttactttta
    12901 ttttttccat acacccacgt ccttttttac attataaatg accttgcgct tgatggaata
    12961 aaaagagcat gatcatgcta tacacaccca ccgaccacgg tttcaagaaa atcacaatct
    13021 gcataaattc cctaaaagat aatttcgtca aaccagctac tagacagatc aagtcgtctg
    13081 gcgcaaaggg aagcagcaag gtaacaacta gtactttttt aatatctttc gtttttcttg
    13141 tcagcaattc atcaaatttc tgatactttt ttggcgataa aataagctgg acaaaggcta
    13201 cgccatagta acggacaagc aaaaatccga ttgcttcccc aatcactagg ccaatacaac
    13261 tatataaaag cccaaatcca ttgccaaaca tcagcatgcc cgctactgag gagatccctc
    13321 ccggcaaaat aggaatgatc acttggatca cttgcaacag aacaaatatc atcactgcat
    13381 acttgccaaa ttgctggata aacgcttgca aagacgtgct cgatcgaaaa atcccatgat
    13441 gataagcata aagaatcaac acaaagaaaa aaaacagacc aataatgggc atgatctgaa
    13501 gacatttttg taaaactgat ccttttttag acatctatat tccccacatc ttttttcatt
    13561 tattaaaact gcctcct
//
LOCUS       DAZULY010000062        13398 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000062 DAZULY010000000
VERSION     DAZULY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.063"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(14..712)
                     /locus_tag="ACG81A_001161"
                     /pseudo
     CDS             complement(14..712)
                     /locus_tag="ACG81A_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            791..967
                     /locus_tag="ACG81A_001162"
                     /pseudo
     CDS             791..967
                     /locus_tag="ACG81A_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="ACG81A_001163"
     CDS             complement(1143..1712)
                     /locus_tag="ACG81A_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS5014540.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="ACG81A_001164"
     CDS             complement(1786..2109)
                     /locus_tag="ACG81A_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014541.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="ACG81A_001165"
     CDS             complement(2263..3075)
                     /locus_tag="ACG81A_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014542.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3402..4277
                     /locus_tag="ACG81A_001166"
     CDS             3402..4277
                     /locus_tag="ACG81A_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFS5014543.1"
                     /translation="MSKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4291..4614
                     /locus_tag="ACG81A_001167"
     CDS             4291..4614
                     /locus_tag="ACG81A_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014544.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4611..5363
                     /locus_tag="ACG81A_001168"
     CDS             4611..5363
                     /locus_tag="ACG81A_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFS5014545.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="ACG81A_001169"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="ACG81A_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="ACG81A_001170"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="ACG81A_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFS5014546.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="ACG81A_001171"
     CDS             complement(9827..12013)
                     /locus_tag="ACG81A_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5014547.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="ACG81A_001172"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="ACG81A_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFS5014548.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="ACG81A_001173"
     CDS             complement(12235..12675)
                     /locus_tag="ACG81A_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFS5014549.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="ACG81A_001174"
     CDS             complement(12749..13288)
                     /locus_tag="ACG81A_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS5014550.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2380 g         4446 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaataa
//
LOCUS       DAZULY010000063        13389 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000063 DAZULY010000000
VERSION     DAZULY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.064"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            40..1938
                     /locus_tag="ACG81A_001175"
     CDS             40..1938
                     /locus_tag="ACG81A_001175"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFS5014551.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2237..2956)
                     /locus_tag="ACG81A_001176"
     CDS             complement(2237..2956)
                     /locus_tag="ACG81A_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFS5014552.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    3146..3193
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            3293..3625
                     /locus_tag="ACG81A_001177"
     CDS             3293..3625
                     /locus_tag="ACG81A_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014553.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3612..5615
                     /locus_tag="ACG81A_001178"
     CDS             3612..5615
                     /locus_tag="ACG81A_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFS5014554.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5634..6104
                     /locus_tag="ACG81A_001179"
     CDS             5634..6104
                     /locus_tag="ACG81A_001179"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFS5014555.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6125..6709
                     /locus_tag="ACG81A_001180"
     CDS             6125..6709
                     /locus_tag="ACG81A_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFS5014556.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6724..7710
                     /locus_tag="ACG81A_001181"
     CDS             6724..7710
                     /locus_tag="ACG81A_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFS5014557.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            7700..8011
                     /locus_tag="ACG81A_001182"
     CDS             7700..8011
                     /locus_tag="ACG81A_001182"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFS5014558.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8033..9814
                     /locus_tag="ACG81A_001183"
     CDS             8033..9814
                     /locus_tag="ACG81A_001183"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFS5014559.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9807..11183
                     /locus_tag="ACG81A_001184"
     CDS             9807..11183
                     /locus_tag="ACG81A_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFS5014560.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11190..11825
                     /locus_tag="ACG81A_001185"
     CDS             11190..11825
                     /locus_tag="ACG81A_001185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFS5014561.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11855..13210
                     /locus_tag="ACG81A_001186"
     CDS             11855..13210
                     /locus_tag="ACG81A_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFS5014562.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
BASE COUNT         4336 a         2350 c         2987 g         3716 t
ORIGIN      
        1 tttataatgt tgagtatacc aatttttgga ggcgttttaa tggaccgcta tttagattta
       61 ctttcagaga agttccctac aacggaagat gtcatcacgg aaatcatcaa tctcgaagca
      121 attttgcagc ttccaaaagg aacagagtta tttttgagcg atatacatgg cgaatttcct
      181 gcttttgatc atattctgcg tatcggttca ggaaatctta aagaaaagat caaagacctt
      241 ttctctgaac aaatgacgga aacggagatc agccaattca ctatttttac ggcttatcca
      301 gaatatgtgt tgacgactga ttggtacaaa aaacaagata aatcccagct gatccatcgt
      361 ttgatcgatc ttttacggtt tacgacagtc aaatacactc gatcaaaagt tcgaaaagga
      421 ttgccgaaag aatatagcta tatcattgag gaactcttgt atatcgatga tcgtatcaac
      481 gggaaaaaag actatgtaaa aaaaatcatt gaacaacttg tcttgatgaa ggaagaagaa
      541 aaatttttaa caaaacttgc gcagacgatc caaaaatctg tcatcgatca tttgcatatc
      601 gtgggtgata tttttgatcg aggaactcaa gcagccaaag taatggatcg aatcatggat
      661 ttttcctctg tagatatcca gtgggggaat catgatattt tgtggattgg tgcgtattgc
      721 ggctctgagg catgtttatt gacgctttta cggattgccg cacgctacaa ctatttattt
      781 gaattagaga aagaatatgg attaaattta cgtccattat tttcttttgc ccatcaaacc
      841 tatcagaaga atccagtatt tacccctaaa aacaggaaag atctatctga aagagagcaa
      901 gaagaattag aaaagatcca tcaagcactt tctatcctcc aattcaaatc agaacatcag
      961 ttgttgaatc gacgacctga atttaaaatg gacgatcgta aattgttgga aaaaatcaac
     1021 tatgaggaat caacgatcgt tttagaaggt cagctctatg agttgaaagg gacttgtttt
     1081 caaacgatcc agccagatga tcctaaaaaa ttcttggaag aggaacaaaa agtgatcgat
     1141 agtttgatgt attcattcca gcactcgtta agaatgcaaa aacacatgac gtttttgatc
     1201 gaaaaagggg gaatgtatct cacgaacaat cagcatgtac tgttccatgg ctgtatacct
     1261 gtagatgaaa aggggcagct cttagcattt gaattagacc aatcttatcg cggaaaagaa
     1321 ctgctgggat tttttgaaaa acagataact gaaagcgcaa aagatttgac tgcaaatgaa
     1381 gatttggcga ccgatttgat ttggtatgcg tggagcgggc cattttcgcc tttattcggc
     1441 aagagtaaaa tggcaacttt tgagcgctat tttctcacag agcctcatac gcacgtcgaa
     1501 aaaagcaatc cctattatca cttgcgagat gaagagtggt tcagtgaaaa aattttggca
     1561 gaattcgatg caaaagaaga aggttcagca atcatcaatg gacacacgcc agtaaaagta
     1621 aaaaaaggag agtcaccgat caaagcaaac ggaaaagcat ttgttattga cggaggtcta
     1681 tccaaagctt atcaaaaaac aaccgggatt gctggctact cattactatg caattcttac
     1741 ggtttccaaa tcgtgacaca ttatccgttc ttgcctattg aacaacaatt tgcaaaaaaa
     1801 tctgatcaaa cgaatgtaaa aaaagtgatc gagcagcagc tcccacgcaa acttaatcgg
     1861 aatacgacaa aaggaatgga attacagcaa cagattactc aactccaaag gctgttggat
     1921 tatcgaaagg acgattgaga aaaatgctag ccaacattgc tcaatagact aaaaagcgac
     1981 tgcaacaaaa ggcgtgtcgc agtctccttc ggaattagct caaaaaacgg taaaatatta
     2041 gaagctattt ttctgtttat ttctctaata ctcaggtcag ggcgctgatt tcacgacctc
     2101 ttccttaaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccaacaaaca
     2161 tgagaagctg ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac
     2221 ctaatcagaa aaacgattaa aattcattca caaagaaatc gaataaacgc tgttcagatg
     2281 aatctacacg atgagaaagt tccggatgcc attggacacc aaggattttg tgcatattat
     2341 ctttactttg gactgcttca atgagtccat cgttggaaaa ggcgatggct tttaacaaag
     2401 gagaaagatc tttgactgcc tgatgatgat aggaattcac aaagtatgaa tcagggagaa
     2461 ggtcacttaa taaactgtcc gaaacgattt tgacttcgtg agttgcaaac tgcggggcag
     2521 tgggctgttg ttcgtgtttt acggaccatt ttggatataa tgaaagatct tgataaagtg
     2581 ttccctccaa cacgacattc aatagttgaa gtcctcggca gactgcaaaa atcggttttc
     2641 tttgtttcaa tgcttccaag atcaatgcct gttcgaattg gtctcgttga atatttgttt
     2701 ccgtcagttt tggatgaggg tcttccatgt aaagttgcgg actaacatcc tgaccgcctg
     2761 caagaatgag tttgtcgatt ttctcaatta gttgaggcgc tgaatctgga gacatgactg
     2821 gcagaataag aggtaatcct tgagcatcta aaactgcatc cacaaatccc tgcggtgtat
     2881 agcttacttg atttccttgg aaagtatctg tcgctcgaat cagttgattc ccggcaatgc
     2941 cgatgattgg tttcattttt attcccccta aagtagacta attgtaacaa ggatttaaaa
     3001 aaatttaaac tatgtaagac ttgcccctat acttatctgt tcttgctgtg ttactatctg
     3061 taaagagaat tttcgtgtga aattcatgac gatagttgaa aatattgctt aatttgttat
     3121 aataaaaagt aagcagaaat aaaaaggttg ggcgtaagct tcaaggatct agttcttcaa
     3181 ggggtgagaa ggacatttta ttgaaggggc ctgcctatac taagttatct ttagaggtac
     3241 atactttcaa tgaaaaaaga aagcatgtac ctctttttta aatcgaaagg aagtgaagca
     3301 agtgttagaa gctttggaac aaatccgaaa agcagaagaa gaaaatgaga agcgtgctcg
     3361 aaccctacgg cagcagttga agacctatgc agaagaaaaa gagcaagaat tggagataat
     3421 ccaaacgaac agtcggcaac agctgaaaca gttggtagaa gaaaaagaac aattcgaaaa
     3481 gcaagaatta gaaaaagaaa aaatgaaatt gaaagcagaa gcagacaagg tgcaagcaac
     3541 tttgaaagac caatatcaaa agcatcagag tcaagcaatc ggtgcaatca ttgaaagggt
     3601 gaaagaaact tatggccgtc actaagatgg aaaaagtgac cctcatttca gataaaagaa
     3661 atcaggagag gattttacaa gcagtacaag gaatccaaaa tgttgaaatc cgggatctgt
     3721 atcaaaatag aacaaacaac cagtgggtag aaaaatattt tccagatacc acgattattg
     3781 ataaagaagc aaaattaagc gctttaggca atcggctgaa agacatccaa gaagctatcc
     3841 aattcataca acaccacggc gatagccgtc aaaagaaact ccatttgaaa cgtcaagaac
     3901 tttccttaca agacttggaa gcaaattatt ccgaagaaaa tttcatgaat aaattagaag
     3961 aagtccttga tttaaaagaa aagtgggaac agctaactga aaaacgtgat cgtttagaag
     4021 aagaggaaga atggcttctt cattggcaac agctggatat tgctccaagt atgtatgcaa
     4081 gtaagacgac catttttgag atggggactg tgagtgcagc aaattttgaa agtttcaagg
     4141 aagaactaag taagcttgat gacgtttata tggaagaaat agattatacg ccaaaacaag
     4201 tttatctttc gtatgttgct ttaaaaaaat cagttgctgc tatagaagaa attgctggga
     4261 aatacgggtt caataaagaa acgtatcctt atgaacaaaa tcctaaagaa aaattgcaag
     4321 aaaccaaaca gcaattatcc attgttcacg agcagcaaaa aaagataacg acagcgttag
     4381 gtcagtgcag cggctatatc caagaatttg aatgggtaga agaagtcaca cttgccgttg
     4441 cagaacggga aaaagtaaaa gaacggttta tccatgccac ttatctgatc gttttgcaag
     4501 gttgggtaga tgcagaagaa aaacagaatt tgatgcatgt gttaaacgaa actgtatcag
     4561 aagaagatat atacgttagt ttcgaaacac caaactcaga agagattcag caggaagtcc
     4621 ctactaaatt gaaaaaccat ccgcttatcg aaccttttga attgctgact gaaatgtaca
     4681 gtctgccaaa atatgaagaa gtagatccaa cgccttggat gacaccattt tatcttgtct
     4741 tcttcgggat gatggtcgca gatgttggtt acggattgct gatgctcatt gggacaatac
     4801 tagctcaaaa actatttgtt ttaccccgcg ggatgaaacg tttcgtaaaa ttctttgaga
     4861 ttctctcggt accatcgatc atctggggat tgatctacag ttcatttttc ggacaggcat
     4921 taccaaaaga actatttggt atccgcttgc ctttcccaat cctatctacg acggatgacg
     4981 tcaatacgat tttgatttta tccgtgattt tcggtctgat acagatcttg gttggattat
     5041 tcgtggcagc aaaggaaaat atcaggagga aagattactt gagtgctgtc agtgatggtt
     5101 ttgcctggca agggatcctg attgggatcg ttattgcatt gattggtgtc atgttgataa
     5161 aaaatacagc atttgtttat cttggcgcgg ggctatcgat cattgctgct ttatgtatct
     5221 taatcgtgcc gattatccaa tcgccgtcta aagtcaaggg tgctgccaaa ggagcgtata
     5281 atctttacgg attgactggt tatatcggtg atttagtcag ttatacacgt ttgatggcac
     5341 taggtatttc aggcggaagt attggcgcgg cattcaatat gctagttgct ttcatgccgc
     5401 cagccgctcg attcagtgta gggatccttt tgatcatcgc tttacacgca ctgaatatgt
     5461 ttttgacact gttaagtgcc tatgtccatg gtgcacgatt gcagtatgtc gaattttttg
     5521 gaaaatttta tacaggaggc ggacgtgcct ttgacccgct gaaaacggcg gaaaaatacg
     5581 tcaacatcaa tcataagaaa aaaaacaata aataattgga ggatttttat acaatgatgg
     5641 attacttaat tagtcaaaat ggtggaatgg tatttgcagt attagcgatg gcaacagcga
     5701 cgattttttc agggattggt tcagctaaag gtgttggaat gacaggtgag gcagcagcag
     5761 cgttaacaac aagtcaaccc gaaaaattcg gacaggcttt gattctgcaa ttgcttccag
     5821 gtacgcaagg gttgtatgga ttcgttatcg cctttttgat ttttatcaac ttaggaaatg
     5881 atatgtcagt tgtacaagga ttgaactttc taggcgcttc tctgccgatt gcttttacag
     5941 gtttgttttc aggtatcgcc caagggaaag ttgcagcagc aggtatccaa attttagcga
     6001 aaaaacctga acatgctaca aaaggaatca tttttgctgc gatggttgaa acatatgcga
     6061 ttttaggttt cgttatttcc ttcctattag tattgaatgc ctaagcataa aggaggaaaa
     6121 gcagatgaac gccattgaaa aaatcatctc tcagatgaat gaagcagcag agcaagaaag
     6181 agcggcgtta gagcaagaag agcgaatgaa gatcgatcaa aactttgaac aaaaacgtac
     6241 gcaaatcgaa acagaacatc aaaaacaaaa agaaaaacaa atcgaattac ttgaaaaaaa
     6301 ataccgtcag cttaggaatc gccaacaagt agaagtacga caagaaaatt tgaatgcgaa
     6361 gcaggaattt ttacgacgat tgtttgcaga tgcaatagct gaaatggaga attgggatga
     6421 atctgaacaa atccagttta tcaaaaattc attgtattca ttacctttga caggcaaagt
     6481 aacattcatt gctggagaaa aatcagcagc ttatctttct cagacattgc tggatgaatg
     6541 gaacaatgaa cttcccttta tgatggtttt aagtgatgaa actgttgctg atcaagcggg
     6601 ttttctgata aacgatcaag gagttcagta taattttctt ttcagcagcc ttgtacaaga
     6661 tatccaagga acgatgagtt ttgagattgc caatcaatta ttcgaataag gagggatcag
     6721 tgtatggaat accacgaact aaatcccttg attcgcggaa gagagcttga acttttgtcc
     6781 aatgagacat ttgaacggat gattcaaaca aattctgtcg aagcacttgg tgaaatattg
     6841 aaatccacga tttacagtcc atatatttat gacggatatg aatatgactt cgaacagaat
     6901 cttttaaagg agcagagccg tctatttcgc tggttaaagg aatcagcacc ggagcctgaa
     6961 atcgtttgga tctatacgat gcgctatact ttccataatc tgaaggtttt gacaaaagcc
     7021 gagatgacaa atcaaaatct ggaccatttg tatatcgacg atggatttta ttcattagaa
     7081 accttgaagg atgccattca tacgcaagtt tcaaatgaac tccctgaaag cattatggac
     7141 agtatacgag aagtacgtga ttattttgaa gagtctacca ttcttcaggg aattgacgtg
     7201 atttatgatc gacagttttt gacagagcag cgacggcttg ccgataagct aggatatcaa
     7261 gaattgctga cagaagtcat tgcttttatt gacttgacca atatcacaac gatggctcga
     7321 ggaatattgc agaaaagaac atatgggttc atgacgacgg tactttccag ctcgggaagt
     7381 atcgaaaaag aagtattttt gaactttgta gataaagata tggaagatta cacgaatttt
     7441 ctattagaga cagattatgc tgattttctg cgccctgtca tccgtgaggg aaatcttgat
     7501 ctcgtaaagc ttgaacagtt gaaagatgat tatctaagtt cattgtatca agaagcacaa
     7561 acccaggctt tcggaccact gccactacta gcctttttga acgcaaaaga ggtagaaagc
     7621 aaaaaccttc gtttactggt tatcggcaaa agaagccatt tcgaaccaga aacaatcaga
     7681 aagcgggtga gacaagtcta tgacgcataa gatcggtgta gttggcgaca aagattctgt
     7741 tttgccattc aaattgtttg gctttgatgt tcgttatggt acaaccaagc aaaccgttcg
     7801 tcaggcaatt gaagaaatgg caaagaatga gtatggcgtg atttatgtca cagagcaatg
     7861 tgcagcattg agcccagaaa cgatcgaacg ttacaaggaa caaatgatac ctgctatcgt
     7921 attgattccc aatcatcaag gatcattagg gatagggctg gaagaaattc aaaaaaacgt
     7981 agaaaaagca gtaggtcaaa atatcttata gtatgaaaag gagatagaaa ttttgcaaat
     8041 tggaaaaatc ataaaagtct ccggacctct tgtaatggct gagaacatgt ctgatgccag
     8101 tatccaagac atgtgtctag tgggagatct aggagttatc ggagaaatta tcgagatgcg
     8161 tggcgatgtg gcgtctattc aagtatatga agagacttct ggaattggtc caggcgaacc
     8221 ggttcgctct actggtgaag ctttatctgt tgaattagga ccagggatca tttcacaaat
     8281 gtttgacgga atccaacggc cattagatac ctttatggaa atcacacaaa gtaatttctt
     8341 agggcgcgga gttcaattgc ccgcattgga ccatgagaaa aaatggtggt tcgaaccaac
     8401 agtagaagcc ggagaaacgg tgagtgctgg agatgttatc gggatcgtag aagaaacaaa
     8461 agttataaaa cataaaatca tggtgccaaa tgggataaaa ggaacgatca aaacaatcga
     8521 aaaaggttca ttcactattg atgaaacagt atgtgtcatt gaaaccgaaa atggtgacaa
     8581 aaatttgacg atgcttcaaa aatggccagt aagacgtagt cggccaatca aagaaaaatt
     8641 aaacccggat gcacccatga tcactggaca gcgagtgatt gatacattct tcccagtgac
     8701 aaaaggagga gcagcagctg tacctggtcc ttttggtgca gggaaaacag tcgttcagca
     8761 tcaaattgca aaatgggccg atgtggacat ggttgtctat gtgggatgtg gcgagcgtgg
     8821 aaatgagatg acggatgttt taaatgaatt ccctgaattg attgacccta atacaggaga
     8881 atcgttgatg gaacgtacgg tattgattgc taatacatca aacatgccag tagcagctcg
     8941 agaagcttct atctatacgg gaatcacaat tgcggaatat ttccgtgaca tgggttatga
     9001 cgttgctatc atggcagact ccacttcacg atgggcagaa gcacttcgtg agatgagcgg
     9061 acgtttggaa gaaatgcctg gtgatgaagg ttatccagct tatcttggtt cacggcttgc
     9121 ggaatactat gaacgatctg gaagagtcat tgcattagga tcagaaggtc gcgaaggaag
     9181 tataaccgca atcagtgctg tatccccgtc tggaggggat atttcggaac cagtcacaca
     9241 aaatacattg cgggtcgtaa aagtattctg gggtcttgat tccagtttag cccaaaaaag
     9301 acattttcct tcgatcgatt ggatacaaag ctattctctt tattcaacag aagtagggaa
     9361 gtatatggat catatccttc aagctgactg ggcatccatg gtcaccgagg gcatgcgtat
     9421 tttgcaggaa gaacagcagc tgaatgaaat cgtgcgtttg gtagggattg attccctttc
     9481 agataatgac cgtctgacat tagaagtggc aaaatccatt cgtgaagatt atttgcagca
     9541 aaatgctttt gatgatgtcg atacattcac ttcaagagag aaacaattca aaatgttgaa
     9601 gatgatcttg acctttggtg aagaaggacg ccgtgcgttg tcgttaggtg cttatttaag
     9661 tgagatcatg gacggtaccg tatcgattcg tgaaaggatc ggacgaagca aatatatccc
     9721 ggaaacagaa ttagaaaaac tagatcaaat tattggtgaa atcaagcaaa cgatgcaaac
     9781 gatcgtttca gaaggaggca tgagcaatga ttaaagaata tcgtacgatc ggtgaagtcg
     9841 tcggtccatt gatggcggtt gaaaaagttg ctggtgtcaa atatgaagaa ttaatcgaag
     9901 tccgtatgca aaatggcgag attcgtcgtg gacaagtatt ggaagtccaa gaagataaag
     9961 caatggtcca aatattcgaa ggaacaagtg gtatcaattt acgtgactct tctgtacgat
    10021 ttttaggcca tcctttagaa ctaggtgtat cggaagacat gatcggtcga gtattcgatg
    10081 gtttgggacg accgaaagat aacggaccag agatcctgcc tgaaaagcat ttggatatta
    10141 acggagaagt aatcaatcca gtagctcggg attatccaga tgagtttatc caaacaggta
    10201 tctctgcaat cgatcatttg aatacattag ttcgtgggca aaaattacca gtattttctg
    10261 gctctggtct tcctcataaa gaattagctg ctcagatcgc gcgacaagca acggtactcg
    10321 attcatcaga tgatttcgcc gtagtctttg ctgctattgg aatcactttt gaagaagctg
    10381 aatttttcat ggaagatttt cgtcaaacgg gcgcaatcga ccgttctgtc atgtttatga
    10441 atttagcaaa cgaccctgca atcgaacgta tcgctactcc tcgtatggct ttgactgctg
    10501 cagaatatct tgcttatgaa aagggaatgc atgttttggt tatcatgaca gacatgacca
    10561 actatgcaga ggcattgcga gagatttctg cagcacggcg agaagttcca ggaagaagag
    10621 gatatcctgg gtatctttac acgaacttag ctactttatt cgaacgagca ggaagaatcc
    10681 gcggactaaa aggatcggtt actcaaattc cgattctaac aatgccagaa gacgataaaa
    10741 ctcatccaat ccctgacttg actggttata ttacagaagg acagatcatc ttgactagag
    10801 agctttacaa gagtgggatt cagccaccaa ttgatgtact cccgtctctt tcgcggttaa
    10861 aagataaggg aactggaccc ggaaagacaa gagaagacca tgcagctacg atgaatcagc
    10921 tttttgcagc ctatgcacaa ggaaaacagg caaaagaatt agccgtagtt ttaggtgaat
    10981 ctgctttatc tgatgtagac aagatctatg cgaagtttgc tgagcggttt gaaaaagaat
    11041 atgtcaatca aggattctac acgaaccgtt cgattactga aacacttgat ctagggtggg
    11101 aactgctatc gatgttgcca agaaccgaat taaaacgaat caaagacgac atgctggaca
    11161 aatatctgcc agaagggaag tgagttctga tggcacgatt gaatgtgaat ccgacccgca
    11221 tggaattgac tcgattgaaa aaacagttga caactgccac aagagggcat aaattattaa
    11281 aagataagca agatgaactg atgcgtcaat tcatcctgtt gattcgaaag aacaaccagc
    11341 tgcgtcaaga aatggaagag tctatgacag aagcaatgtc tgattttgta ttggcaaatg
    11401 cttcgatcac ggaacctttc atcgaagaat tatttgtttt gccggcggaa aacgttgatt
    11461 tgtctatcgt agacaaaaat atcatgagtg taaaagtacc tgtgatgaat tttgaatatg
    11521 atgaacagct gacagacaca cctttggaat acgggtattt gaattccagt gccgaactgg
    11581 atcggtcaat cgatcggttt actaccctgc ttccaaaact tttggaactg acagaaatcg
    11641 aaaaaacttg tcaactgatg gcgagtgaaa tcgaaaagac acgtcggcga gtcaatgcat
    11701 tagaatatat gacgatccct caattagaag aaacgatcta ttatattcgt atgaagctgg
    11761 aagaaaacga gcgcgcagaa gtaacaaggt tgatcaaagt aaaaaatatg ggtaccactg
    11821 actaagtggt atcttttttt aaaagaggaa aacgatgatt aaacaaagca aacaaataaa
    11881 gaaacgtttg ccggctgttc agcttctcgc attaggattt tttctcctga ttttagttgg
    11941 cggcggccta cttaccctac cattttttag taatagtggg catgggacaa attttatcga
    12001 tgcgcttttc actgcaactt ctgctgtctg tgtaacggga ttgacaacat tgaatacagc
    12061 agagcattgg aacagtgctg gtcaattttt gatcatgctt ttgattgaga tcggcggtct
    12121 tggttttatg atggttccga ttctatgctt tgcattgata aagaagaaag tcagtctcag
    12181 cacacgtatt atattaaaag aagcgctgaa tctagaagaa gtatcaggtg tgatgaagct
    12241 catgctttac atcctaaaat tggcctttgt tatccaattg attggtacgg ttgctttatc
    12301 ttttgtattt gtgcctgaat ttggctggtt gaaaggtatc tggtacagtt tgttccatgc
    12361 aatctctagt ttttgtaatg cagggtttga tttgttagga gacagtttag ccaatcacca
    12421 gcaaaatgtt tatttgatca tggtggtatc tgctttgatc atttctggtg ggttaggctt
    12481 catcgtatgg cgtgatttac tggaatatca tcagaaaaag aagatgacga ttcattcaaa
    12541 aatcgcatta accgttacag gtaccctttt attaggcgga ttcgttgtgt tctttttcac
    12601 agagcgcaat ggggcaacgc tagtggaagg gaatatgttt caacgattgg caaacacctt
    12661 ttttatgagt gtgacaccta gaacggctgg atattattca gttgattacc tccatatgag
    12721 ccatgcaggt ctgattttaa cgatgttttt gatgtacatc ggcggaacat ctgggtcgac
    12781 ggccggcgga ttgaaaacaa ctacgttagg tgtgttgctg atccagatgc gttcgatgtt
    12841 gaaaggacgt acgagagcgg aagcatttgg ccgtaccatc cgacaaggcg cagtgttgcg
    12901 ggcacttact ttattttttg ttacgttgac gttgtgtgta gtcgccatca tgattttatc
    12961 ggtgactgaa acgataccta aaacttctgg catcgaatat attgcttttg aggtcttctc
    13021 tgcgtttggt acagtaggtc ttacgatggg attgacgcca gacttgactg caatagggaa
    13081 attgatcatt atagcgctta tgtacattgg acgagttggt gtattgactg tggtcttctc
    13141 tctgatgaca aaagcacaaa gaacacaatc aaaatacaaa tatccagaag aaagtgtcat
    13201 gattggataa tttcatcttc tgacagaaac ctagtgattt agtaaaataa gaaaataaaa
    13261 taatttttct tgcttcttgg atactttaag tgcaaaatgt tcgtttattt tttgaggcac
    13321 tacccgttgt tgaacctcgg gtagtgcctc ttttttattg aaaggaatca ataagatttg
    13381 attccttta
//
LOCUS       DAZULY010000064        13270 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000064 DAZULY010000000
VERSION     DAZULY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.065"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..1475
                     /locus_tag="ACG81A_001187"
     CDS             138..1475
                     /locus_tag="ACG81A_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFS5014563.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="ACG81A_001188"
     CDS             1523..3652
                     /locus_tag="ACG81A_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFS5014564.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="ACG81A_001189"
     CDS             3627..4316
                     /locus_tag="ACG81A_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014565.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="ACG81A_001190"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="ACG81A_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="ACG81A_001191"
     CDS             complement(5546..5740)
                     /locus_tag="ACG81A_001191"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014566.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="ACG81A_001192"
     CDS             complement(5790..6230)
                     /locus_tag="ACG81A_001192"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFS5014567.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="ACG81A_001193"
     CDS             complement(6234..7079)
                     /locus_tag="ACG81A_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFS5014568.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="ACG81A_001194"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="ACG81A_001194"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFS5014569.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="ACG81A_001195"
     CDS             complement(8703..9650)
                     /locus_tag="ACG81A_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5014570.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="ACG81A_001196"
     CDS             complement(9873..10223)
                     /locus_tag="ACG81A_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS5014571.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="ACG81A_001197"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="ACG81A_001197"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFS5014572.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="ACG81A_001198"
     CDS             11646..11966
                     /locus_tag="ACG81A_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS5014573.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="ACG81A_001199"
     CDS             11988..12452
                     /locus_tag="ACG81A_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5014574.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /gene="ytpR"
                     /locus_tag="ACG81A_001200"
     CDS             12657..13262
                     /gene="ytpR"
                     /locus_tag="ACG81A_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFS5014575.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
BASE COUNT         4163 a         2454 c         2773 g         3880 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg
//
LOCUS       DAZULY010000065        13223 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000065 DAZULY010000000
VERSION     DAZULY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.066"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1256)
                     /locus_tag="ACG81A_001201"
     CDS             complement(<1..1256)
                     /locus_tag="ACG81A_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5014576.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERK"
     gene            complement(1249..2148)
                     /locus_tag="ACG81A_001202"
     CDS             complement(1249..2148)
                     /locus_tag="ACG81A_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5014577.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2293..4071)
                     /gene="uvrC"
                     /locus_tag="ACG81A_001203"
     CDS             complement(2293..4071)
                     /gene="uvrC"
                     /locus_tag="ACG81A_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFS5014578.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4253..4567)
                     /gene="trxA"
                     /locus_tag="ACG81A_001204"
     CDS             complement(4253..4567)
                     /gene="trxA"
                     /locus_tag="ACG81A_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFS5014579.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4654..7014)
                     /locus_tag="ACG81A_001205"
     CDS             complement(4654..7014)
                     /locus_tag="ACG81A_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFS5014580.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7129..7676)
                     /locus_tag="ACG81A_001206"
                     /pseudo
     CDS             complement(7129..7676)
                     /locus_tag="ACG81A_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            complement(7734..8171)
                     /gene="zapA"
                     /locus_tag="ACG81A_001207"
     CDS             complement(7734..8171)
                     /gene="zapA"
                     /locus_tag="ACG81A_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFS5014581.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8373..9290
                     /gene="rnhC"
                     /locus_tag="ACG81A_001208"
     CDS             8373..9290
                     /gene="rnhC"
                     /locus_tag="ACG81A_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFS5014582.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9546..10457)
                     /locus_tag="ACG81A_001209"
     CDS             complement(9546..10457)
                     /locus_tag="ACG81A_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS5014583.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(10860..11438)
                     /locus_tag="ACG81A_001210"
     CDS             complement(10860..11438)
                     /locus_tag="ACG81A_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5014584.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(11458..12126)
                     /locus_tag="ACG81A_001211"
     CDS             complement(11458..12126)
                     /locus_tag="ACG81A_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5014585.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(12141..13211)
                     /locus_tag="ACG81A_001212"
     CDS             complement(12141..13211)
                     /locus_tag="ACG81A_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5014586.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
BASE COUNT         3750 a         2846 c         2186 g         4441 t
ORIGIN      
        1 ttttttcgtt catttctcat aattgaaatc gattgtttca atgaactcca taatccgcct
       61 tcgctataca cgagtacatt cgatttgtac atgtttgcag acaagatcat caataagaat
      121 gttacggcta caagtaccaa caaggaaatc aatgctccac ccataccgac tgtctcattt
      181 gctaagcgaa ccggcataat atatgaagat aagaatggaa tatacgaagt gatccgaata
      241 acgatattgt ttggatctga agctcctagt atcaatccaa gcatatatcc gcctagagat
      301 agataagtca ctggcaaaat tgcttttgct gtatcttctg ctttgttgac caaagagcca
      361 cataaagctg ctaaaacaga aaaaatcaaa atgccaaaga tcatgaatag aagagaatac
      421 cacaagaatg gtccaatcaa gttatccaat gagataccgt ctaatatatt tttgaccatt
      481 tccatatctt tgaattgact gtatcctata ccaaaaataa taccgtataa tgccatttgc
      541 gttaatgcaa caagtaaaac acctgttagt tttccgtaaa aatgcttttg tgcagtcgta
      601 ctggacagga tcacttccat gatccgcgtg cctttttcag aagcgatctc ttgtgcaatg
      661 atctgcgcat acgtcaaaat aataatgaac aagacaatcg ttgctacata acttacgaca
      721 tattggactg tcgagttatc ttctccaact gtcatttttc cttttgaatc aaaattgact
      781 ttttgtcgag tcaaacttgc ttgttggctc aagctagcta cttcttctgg tgaaatgcct
      841 aattgacttg ctcgaagcat cgattgcaag ccagtcagtt gttgttgtat cagtagttgt
      901 gtactttgtc ctaatgaatt ttcgctataa agcatcccat ttatcttatc attctcagtt
      961 gagactacta aatacgcatc gattttttct tccgaaagct ctttttgtgc tttttcttca
     1021 gaatcaactg ctttgaactc aaaatcatct gttttgactt ggctcatttg ctgactgatt
     1081 tgctcattat cagccaatac gccgatttta ttgacatcac tattttgttg agcaaagttt
     1141 cctgctacat aaatgatacc cagcatgata aaagggacta atatcatgat caagaaagaa
     1201 atcgacttaa cattttttaa atagacatct ttggtaataa tccaaaactt acgcatcgat
     1261 ttcacctgct ttcaatttga aaatctcttc caaagttggc ggttgctggt taaacatagg
     1321 aatgtagccg aactgagtcg cacgagtaaa gatttctttc ccagcttcag gatcttctaa
     1381 ggtaatctct aacgtattgt cttcatgaag gatcactttt ttcacgcctt cgatttcttc
     1441 tacttcctgt tggcttaaac cagattccaa gaataacttc gtacggccaa atgattcacg
     1501 aatctcatgt acttttccat tcaaaaccat ttttccgttt cgcaacatga tcaaatgatc
     1561 gcagattttt tctacattat ccatattgtg gctagaaaaa atgacacatg aaccatgttt
     1621 tttcaattcc aatattccat cttttagcaa ttcagcattt accggatcta acccgctgaa
     1681 tggctcatcc aaaatgatca atttaggttc atggattaac gtggcgatca gctggacttt
     1741 ttgctgattt cctttagaca aagattttac tttatccgtc tttttccctt ttacctgaaa
     1801 tttttccatc caaaaatcga tttttggttc aatctctttt cttgatttcc ctctaagttc
     1861 tgcaaaataa agcagttggt cttgaatcgt tactttcgga taaagtcccc gttcctccgg
     1921 aagataaccg atgacatcat aatctttctc cccaagatga tggccattcc ataacacttc
     1981 tccttggtct tgtgtcaaaa aatccaagat caaacgaaaa gtcgttgtct tcccagcccc
     2041 gttttggcca attagcccca tgatttttcc atcggggatt tggaaagaga catgatcgac
     2101 agctgtgtag ttcccaaaag ttttaacaag atccttgact tctaacattt cttcactcct
     2161 tcttcaagaa atcacatata tccttagaca ccagacgtaa gcgatttatt atttaccaac
     2221 aaaacgtaaa cgctcttttt gttgtacata aaatgatagg acactttact ttctttgtaa
     2281 agtgcccttt cctcattcct gatgtaattt atcaaatacg ttttgtgcga catttttcgg
     2341 cagtccgatt tcttgtaatt cttctgtcgt ggctgctgtg atatttttca acgatttgaa
     2401 ctctttaagc agcatttttt ttcgttttgg tcctaacctt tcaatttcat ctaatcggga
     2461 agcaaaactg tttttactgc gtagctgccg atggaaagtg attgcgaatc gatggacttc
     2521 atcttggatt cgctggagta agaaaaactc tggtgaattt cgctcaagcg gtactacagc
     2581 caaatctata ccgaatagca actcatttgt tttatgttta tcgttttttg ccatgccagc
     2641 aataggaata tcgattccta attgattttc taagacatct tttgccacat ctacttgtcc
     2701 ttttcctcca tcgatcaaga tcaaatcagg taaaggcaac ccttcgcgaa tcactcgtga
     2761 atatctgcga tagatgactt ctctcattga tgcataatca tccggtccgt tgactgtttt
     2821 tattttatat ttccgatatt cttttcttgc cggttttcca tcaatgaata cgaccatcgc
     2881 agccacagga ttcgtcccca tgatattaga gttatcaaaa gactcgatac gtattggggt
     2941 cgggatattc atcgcgtttc ctaaacgttc aactgctccg atcgtccgtt cctgttttct
     3001 ggcaatcaga tcaaaccgtt cattcaaagc aactgaggca ttcttattcg ccaattcaac
     3061 taattttttc ttttctcctc gttttggttg cagcactttc gtagctaaca aagcttctac
     3121 actgggcttg tcgatcgtat caggaatcaa gacttcttta ggaatgaaat gctcattttc
     3181 ctgataaaac tgaccgaggt aagtcaagaa atcttcttct gcttcgttat agaaaggaaa
     3241 cattgaaaca tctcgttcaa ttagctttcc ttgtcttacg aaaaacactt ggacacacat
     3301 ccatcctttg tcaatcgcat aaccaaatac atctcgatcc agtaaatcag tatttgtcat
     3361 tttttggcga gtcatgattg tctcaatggc acggatctgg tctcggtatt ctgcagcttt
     3421 ttcaaactcc atgttttcag cagctttttc cattttctga tggatctctg actcgatttt
     3481 ttcgtgtcct ccgtttaaaa actttttcac ttcatcaacg attttcgtat agactttcgg
     3541 atctacgtcg aaataataag gacacagaca ttgtcccaaa tgatagtaaa gacaaggctc
     3601 cttcgtctga ctaggtttgc attttctcag aggaaataat cgatcgagga tcttctttgt
     3661 ctcattcgcc gcaccgacat cgggatatgg tccaaaatac agtgctttgt cttttagtac
     3721 tttacgagta atcaccaaac ggggatattt ttcattcgtt atcttaagga aaggataggt
     3781 tttatcatcc tttaacataa tgttgtattt aggatcattt ttctttatca gattgatctc
     3841 taagagcaat gcttcgatat tcgattctgt aacgatgtat tcaaagtctt cgatttcact
     3901 aacaagccgt tccgttttcg tattatggct gccagtaaaa tatgagcgga cacgattttt
     3961 taatattttg gctttgccga cataaatgat cgttccattt ttatctttca tcaagtaaca
     4021 gccgggctga tcaggaagta aagctaattt gtttttgatt cgttcgttca tctggtactc
     4081 cccttcatat tcaattaaag ttattatagc atgtctgtca aaaaaacaat ccagcctgac
     4141 aacgtaatta tactcaaaaa atattaattt gaataacctt aactcaaccg caaaaaaacc
     4201 gaggacggaa ctggattcag tcccaccctc agcttatttt tgctgtaagt ttttataaat
     4261 attgatcgat catttgacgt agttgttctt ttgaatgaac accaacagct ttttctacta
     4321 cttgaccatc ttttttcaac attaatgttg gaatgctcat gataccgaat tgttgaggag
     4381 tttctggatt ttcatccaca tccattttag caattctgaa ttcttcttca tcatattctt
     4441 gttccaactg atccaaaatc ggtgcttgca tacgacatgg accacaccaa gttgcccaaa
     4501 aatcaatcaa tacaagtcct ttatctgttt cttcattaaa tgtcttatct gtaattactt
     4561 gtgacatatc aaagacctcc atttttaaaa tctatgatta gtatagcatt tctatacttg
     4621 gatagcgaac catctgctta cctttagtaa gctttactta aatttaacaa ttgtcgcacc
     4681 atttccgcct tggtttgccg gtgcaaactc aaagcttttg acgctccgat ggtttttaag
     4741 ataatcagta attcccttgc gaagtgcgcc tgttcctttt ccgtggacaa tagtcacttg
     4801 aggataacca gctagaatgg cagaatccaa atattgatct acctcactta gcgcttcttc
     4861 gtatcgtttt cctcgaaggt ccaattgatt cggcacgtga ctactagatt cagatcgtac
     4921 agcatggata actcgttgtg tcggctcttt ttgaggagca acaggcgtca gctcgtcttc
     4981 ggacacattc atttttaata taccgatttc tacttgccac tgattcccgt ttttacgtaa
     5041 aagcgtccct ctttggccgt aagtcgtaac caacacttcg tctcccggtt tcaacgtttt
     5101 ttgttctttc gccttcttca atactttatt tttcttcaaa gtttcttctt gatgtagatt
     5161 tgctaattgg gtctttgcat cgatcaactg atgctctttc acatttcctt gcccaatctg
     5221 ctgttgcatt ttacgaatat cagcaatgat tttttctgct ttttcttccg cctctgacac
     5281 gacttcgttt gcttttttct ttgctttttc catttctttt tcacgttctt caaagaaata
     5341 gctgtaagct tctttcaaat catcgtgaag ttcttgtgcc tcagaaacaa aatgacgcat
     5401 ttccagatat tcggtctctg ccattttccg gcgattctct aaatctgtga tcatttcgtt
     5461 taaatcttgg ctctcatcat tcatcaactg cttcgcttca tcaataactt ctgtatcaag
     5521 acctaatcgg ctagagattt caaacgcatt gcttcgtcca ggaacgccaa tcagcaaacg
     5581 atacgttgga cttagtgtat ccacatcgaa ctccatgctg gcattaatcg tatttgcccg
     5641 attgtatcca taaactttca attcaggata atgcgttgtt gccatcacat aagcagactt
     5701 tttaccaaga tcatctaaga ttgcgattgc tagagcagca ccttcttgcg gatctgttcc
     5761 tgcaccgagc tcgtcaaata ataccagact tttttcattg acttttgata aaatatctac
     5821 agtgttcgtc atatgagaag aaaaggtcga tagactttgc tcaatcgact gttcgtctcc
     5881 tatatccgca aatacttcct caaaaatacc gatttgactt tcttcacctg caggaatcgg
     5941 taatcctgct tgccccatca gttgtaacag acctaaagtc ttcaacgtga tcgttttccc
     6001 acctgtgtta ggtcctgtaa tgacgatggc ttgatagtct tttccgattg tgatgtcatt
     6061 agaaactact ttttcctgat cgatcagtgg atgtcttgct tgtttcaaga cgacatgatt
     6121 gtcttcactg attcccggca cgatggcttt tacttcttta ccaaatcgtg cttttgcatt
     6181 catcagatcc attttaccaa tcacataagc attgtggatg atttcttgac gatggggaac
     6241 aagttcagca gaaagttcac ttaaaatacg ttgaatctca tttcgttcag cgatttggta
     6301 ttgacgcaaa cgattattca aatccaccac ttgttttggt tcaataaata aagtctgtcc
     6361 tgaagcactt tgatcgtgga ctacaccgcc aaatacaccg cggtattctt gtttgactgg
     6421 gataacgtag cggtcatttc gcatggtgat gatcgcatca cttaaatact ttgcattttt
     6481 cccacggacg atcccatcta actgttcacg aacagcttgt tcacttcgcc gaatgttctg
     6541 tcttatgctc tttaactcag gagaagcgtc atcagtcacg cgtccgtctt catcgattgc
     6601 ttcctttaaa cggcggctca aaacagggat cgtgactaac tgatctgccc acatatacaa
     6661 acgtgcaaat tcgatttcac tgtcagaaag atcatcaatg aaacgattca attcgcttgt
     6721 cgtagacaat actctggaaa cttgtgcaag ctccactcca ttcaagtctg ctccgatctc
     6781 gatccgtttc atatggggac ggatattttc aagtttaggt atagggatcc cgcctcgcag
     6841 acgttgcact ttcaatccat cctcagtttc ttgaagccag tttgtgatcg tttgtttctc
     6901 actcactggt accaattctg ccagttcttc ttttccttgt gcagtcacta caaattgtct
     6961 gaccatctgt ttgacttttt caaattccaa tgtttcaagg attcggttat tcattttatc
     7021 accttatctt tcactatgaa aaaagagctg tgccggacat acgctacgca gccttcttaa
     7081 ataaactact ctgtacaaaa aacgaaatat tttccagcac tattttcctt aattcaaaat
     7141 attcgttacc caaagctcat acatcttgtt ggtaagaaat ggcgttctct caacgataaa
     7201 acgagcgaaa ctatgctcgc cgaattgatt ttggaccaca ctaatggaac aaaggcaagt
     7261 aactgcaaaa tcaaaaatat agtaacgtaa gccatgatta gtgacaggat gcctcctgca
     7321 atccagtcta cctgtctcaa gatcggcaca tacgtcaatc catggacaaa aacccccaca
     7381 aatcgggtaa gcagccagcc aattagcaaa ataatcagaa aagcagttcc tgcataaaaa
     7441 gcttcatcta ggtgaaaagc aaatacctga tcaaaaaatg ctaactttga tgttggggtg
     7501 acagctgggt atggaatgta taattccaaa tgacttgcta atggtttgta aaaatgctgg
     7561 gcagcaataa atgacaatac atatccaatc gcgaatatga tttgcaaagc aaacccgcga
     7621 cgtgctccag aaaaaaacgc aattagcaat aaaaataaaa tcagcaaact cagcatgcta
     7681 cgttcctttc tcttctaact tttttaaatt tttcttattt tgataaaagg atcttaatta
     7741 gaagattttt gtttaatttg tccttttcgt ttctcgttta aaatctgttg tgcttccaca
     7801 tgatttttaa tggtatgatc tgcttgacca ttttctttca atacttcccg agcttcgttc
     7861 tcgattgctt caatgcgttt cacacgattt tcaagctctg taaatttgat catttttttc
     7921 tttaattctg ctgtttcttc ttccagctcc aagattcttt cttgtttctt caattgatcc
     7981 gaaagcgcat tgacagccat caagattgct gcttgttcat tatccatttg tggtgataat
     8041 tgtttcaatt ctgctaactg ttcattgatc agcttcgtta cgatatccat atgatgcttg
     8101 gtttcccgtc cgataatagt atatgtttgg ttggcaatca ctgctttgta gcgtgttttt
     8161 tcgtgcgcca tacgaatcat ttcctcctat ttgatgaaag ttttatttag tgtaccatga
     8221 agttggttct ttcaccgttc tccacaaata tttttcacat acttaactat tatatgataa
     8281 actttaaaaa aatgctatgt ctcgcaatct cttagaaaac gaaaagtcgt gagttgcggt
     8341 atagttttat gtataaaaac gaggagaatt ttatgagcaa cgtcattatc aaagcaacaa
     8401 acgaccaact tagtcaaatg aaagcatact atcgctctta tttgttaaat aaacagattc
     8461 cttatactag ctttgctgca aaaaaagacg ggacgaccat taccgcttat acatccggaa
     8521 aagtgatgtt tcaaggtacg aatgcagcaa tagaagctga taaatggggg gaggcacagg
     8581 ataaacaagc caagccagca aaaacagcct cttctttgcc aaaaaatatc agtcaattat
     8641 ccgttttagg tagtgatgag gtcggaaatg gcagctattt tggtccgcta acagtatgtg
     8701 ctgcttatgt gaaacgtgat cagcttgcag aattacgtaa acttggtgtt agagattcaa
     8761 aagagttaaa agatagccaa atcatccagc ttgctgacat cttaaaaaaa accatcccct
     8821 ataaactgct tgtcttatct ccagaaaaat acaatgagat ccaaccaaaa tacaatgccg
     8881 ttcggatgaa agtcgctttg cacaatcaag cgatccattt gctgctaaaa aaaatcgagc
     8941 cagaaaaacc agaagcgatt ttgatcgatc agtttacgcc ggaagcaaat tttaaaaaat
     9001 atgcgcgttt agaaaaaaat caattacaag aaaaactgta cttcgttact aaaggagaac
     9061 aatatcattt agctgttgct gctgcttcta tcatcagtcg tgccagcttt ttggaagagt
     9121 tacacaaagc atcagctgaa gctggaatca cccttccatc aggagctgga acgaaatctg
     9181 accaagtagc ggcaaaacta ttggagaaag gcggcatgcc tatgcttgaa aaatatgcca
     9241 aacttcattt tgccaataca gaaaaagctt tgaagctgct aaaaaaataa tggaactgtg
     9301 acaaaagtca tgtcacagtc ccttgttccg aataaacggt gttctcatgc caactcctcg
     9361 gtcacaagtc acaatagtca tcaaacatga gaagctgttt tttgaaattg ctccttgtga
     9421 cgcggagtta aacggcaggc tcacaacctc ttccttaaac ggtgttcaaa agcttgcatc
     9481 tgcgataata agcccggcca aacgaaaaat atgaggagct atttttgttt ggctgggctt
     9541 atttattact gaaaataagt ggtcaatgct tgtgtcaaac ttgcagcaat ctgctgctga
     9601 taactgtctg ttactaattc ctttaaatca ctatcattat tcatataacc catctcgatt
     9661 aaaatagaag gttgaagatt ttctcgaaga acttgatagt tcccagtgga gattcctctt
     9721 gaatccagag gcagatcagt gaaggagtca gcaattgtct gtgctaatgt ttcctcttgt
     9781 ccatagtaat aatatgtcgt gattccagtt gcttcattcg catattccgt agagtctgcg
     9841 tgtaaactga taaagacatc tgcctttttt tcttcactgt agtcgcatat atctccaagc
     9901 tgtactaatt catctgtagt atgggtcaaa taaaccgttg ctccagcatc ctctagcgcc
     9961 ttttttactt ttatggctgt acttaatgtt atatcttttt cttctgtttg ttcatcattg
    10021 cttaatgccc cagtatcatt tccgccatgc ccaggatcta atacaatcac tgcatcagat
    10081 agctcttccg gttttgtttg tatattcttt gtgactttgg ttccatcttc gagggctaaa
    10141 tatccagtat agccatcatt tgtcttgatc tgataagcat tttttgtttc agaaagaaac
    10201 tccgccagct ctccgccgtt tatagttgca agtggggtag ttagcgagtc atctgtatat
    10261 agtgtacggt cattttttcc gattttgatt aatgtctctt gttctgtatc atcaaatgat
    10321 tcctttgtct ttgtaaccgt actgttcgta ctgctgaccg tttgtccaac tatctttttt
    10381 tgaccaaaaa catctatttg aaaacagata ataatgataa tgattgcaaa tacaaaagct
    10441 ccaattattt ttttcattct tgactctcac ctcgtagatc cagactaagg gtcaaaactt
    10501 aaaatcagct taagtacgca aaaaaggggc tgtaaaagat ataaccgtag aaaataacat
    10561 tactgctaaa atgatttttt tcatttttgg catatcctga aatctccttt tgactattcg
    10621 tggtgattca tctacccaat aaatagtttc cttcattcta caaacaatta atttcttcgt
    10681 tattaaatta aaacacttcc tggaacaccg acataataaa taaaaatata ccctgataat
    10741 catgagagaa taacaaattt gtatttatga aaaactacct atataaaaaa aacaagatat
    10801 aacgatcttc gtcatatctt gcttcttcac ctaactattt ttctccctat ataacagctt
    10861 tacgcttctc ctaatacacc attcaaatat aaatcgatca attgcgccac atttagcggt
    10921 tcttccacag gtcttttcga ccgagtgatg taaggtgcta acactaagaa aaaatgcctt
    10981 gctgcgattt cttttgaaat cccatttctt agcacagatt ggtttgctga aaagatttct
    11041 tcaaaaggtt cacgataatt tttcttccat agaaagaaaa gatgatctct tgtttccttt
    11101 gaaagattgc tttcaagatc gtgaagcatc agaaacaaat cgaccagatg tgtctcaatc
    11161 aaaaactgcg ccatttttgt caaggttact tgaaatcccg tttcgcttgt ttgaacgatt
    11221 tctctcaaag cttttccaac atcttttaag tggttttcta aaactgccgt atatagtttt
    11281 tctttatcac cgaaataatg atagagattg ggttgggtga tcccaacttt ttgtgcgatc
    11341 tctcttgttg aagtgccgag atatcccttg gacataaaca agtcagtcgc ttctttcaaa
    11401 attttttgct gtgtatcttt gttgtatcga tggaccattt ttccacctgc tttttggtta
    11461 ttctttttct ttcaatacac catcttccat cataaatatt ctatcgcatt tgtcaatcag
    11521 ccgtgtatcg tgagtaacca tgatcgttgc tttatttttg tcttttgatt cttttgccaa
    11581 gatatcgact acttctcgtg ccttttctga atccaaacta gcagttggtt catctgctag
    11641 aataatggac ggatcattgt acaatgcacg agcgattgcg accctttgcc gttctccacc
    11701 agatagttct tctggatact tgttgatcaa cttatccacg ccaagctgct caaataattc
    11761 ttttgcttct ttttgacgat gttcatgttt gatcttatct actaataata actgcttttt
    11821 tactgtcaga aaaggaacca gattggaagc ttgcaaaata aagccaattt ctttaaaacg
    11881 tagtttaacg cgttcacttt ctttttgatt actgaattct tgatcattga taaatacaga
    11941 gccactgcta ggcgtctgca atccgcctgc caaagtcaaa aaagtacttt tacctgaacc
    12001 agaaggaccg atgattgcaa caaattcccc tttatcgata ctcatggttg tttctttcaa
    12061 tgctttaact acttggtcgc catcttggaa acttttttct acttctttaa attctattgc
    12121 tttcatgctc ttcctccttc ttatccaatt gccttcaacg gatcgatttt tacgattgtc
    12181 cgaacagaaa atagtgcgcc taacacagct actaccagca tcaacaagct aatgacgcca
    12241 aaaaacagcc aattgctttg gaatggaacc gcatcaggta gaacaaggga tgttcccact
    12301 gttcctaata gaccaagaag aataccaaca aatgataaaa tgaatgtttg tgccactaca
    12361 gaacgcgcaa taaatcctcc tgtgatccct tgtgctttca ttacaccaaa gatattaatc
    12421 ttttgcatcg tcagtacata aatgaaaatc ccgatcacaa tagccgcaat aacgatcaaa
    12481 aatccgatca taaatccaaa agtcaatact tgcgcattat atccaggtaa ttcattgatg
    12541 aatttagaaa ttttgatctg ttctaaatct tcaggtaaat tactgatttt acctctgaca
    12601 actattgcgt taatccgtgc attttcactt gtatcttctt tttcgaagcg gatctcttgg
    12661 taagcattga tggttgtata gagaactggt gagacattga atttggcatg atccgtaaag
    12721 ccagttagtt taaacgtctt gtcactacca gataacttaa ctgtatctcc tacggctaga
    12781 ccatattctt ctttcaaact aatatcgcct actgcctcat catcgttatc aaaagctttt
    12841 ccttcaacaa gattgggcat gatgaattga tttttatcga taccgaagaa gctaacattg
    12901 atttttcctt cttctgtact gtctttacta gtcacgactc caggtgtctg ggcaagatac
    12961 gccactttcc caccttcgac ttcttctgct atcttcttag taatcatgga catccctaga
    13021 tttgaatttg catctttcga aagcacaatc gaatctgcct cccacttgtc gactgctgtt
    13081 cgattatctt gagctaagcc ataagctaac ccagtcaaaa aaaataccag gtaggcgatc
    13141 aaaaacatca cccctgcaac taacgcataa cgtaactttg aatgcataat ttcatttaat
    13201 gctaaaaaca tttaactcct cct
//
LOCUS       DAZULY010000066        12943 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000066 DAZULY010000000
VERSION     DAZULY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.067"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="ACG81A_001213"
     CDS             complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="ACG81A_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFS5014587.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1195..3036)
                     /gene="dnaG"
                     /locus_tag="ACG81A_001214"
     CDS             complement(1195..3036)
                     /gene="dnaG"
                     /locus_tag="ACG81A_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFS5014588.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3218..5533)
                     /locus_tag="ACG81A_001215"
     CDS             complement(3218..5533)
                     /locus_tag="ACG81A_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5014589.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5718..6578)
                     /locus_tag="ACG81A_001216"
     CDS             complement(5718..6578)
                     /locus_tag="ACG81A_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFS5014590.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6637..7269)
                     /locus_tag="ACG81A_001217"
                     /pseudo
     CDS             complement(6637..7269)
                     /locus_tag="ACG81A_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            7436..8197
                     /locus_tag="ACG81A_001218"
     CDS             7436..8197
                     /locus_tag="ACG81A_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5014591.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8223..10814
                     /gene="mprF"
                     /locus_tag="ACG81A_001219"
     CDS             8223..10814
                     /gene="mprF"
                     /locus_tag="ACG81A_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFS5014592.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="ACG81A_001220"
     CDS             complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="ACG81A_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFS5014593.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12557..12913
                     /locus_tag="ACG81A_001221"
     CDS             12557..12913
                     /locus_tag="ACG81A_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5014594.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3713 a         2708 c         2300 g         4222 t
ORIGIN      
        1 tttttctttt aaaataaacc aaacagtttt actgatttct cttctggtca atagtccacg
       61 cttattctaa gaaatctttt aattgttttg aacgagaagg atggcgcaat tttcttaatg
      121 ctttagcctc gatctgacga atacgttccc gagtaacacc aaatactttc cctacttctt
      181 ccaatgttct cgtccggcca tcatctaaac caaaacgtaa gcgaaggaca ttctcttccc
      241 gatcagtcaa tgtatctagt acatcttcca attgttcttt tagtaactca taagctgcgt
      301 gttctgctgg acttgttgct tcctggtctt ctatgaaatc acctaagtga gagtcatctt
      361 cttcaccgat tggcgtttca agggaaacag gttcttgcgc gattttcaag atttctcgga
      421 ctttttctgt tggaagatcc atttccgcac cgatttcttc tggcgtaggt tctcttccaa
      481 ggtcttgcag caattgacgt tggatacgta tcaatttatt aattgtttca accatatgaa
      541 ctggaatacg gatcgtacgt gcttggtcag cgattgctcg agtgatggct tgacgaatcc
      601 accatgtagc ataggttgaa aacttaaatc ctttgcggta gtcaaatttt tcaactgcct
      661 tcatcagtcc catgtttcct tcttggatca agtcaaggaa ctgcatgcct cgtcctacat
      721 aacgtttagc gatacttact actaaacgaa ggtttgcttc tgccagacgc tgtttcgctt
      781 cttggtcgcc ttcttcgatt ttcaatgcta acgctacttc ttcttccgca gttaaaaggg
      841 agacacggcc aatttctttc agatacatac gaaccgggtc attgattttt acaccggttg
      901 gcgcagaaag atcttctgtt tttgcttgtt cagctttttt ctcagcactc ttcaagctgt
      961 gaattgatgg atcaccattt tcatccacca cactgatacc tgcgtcttca atcttttgga
     1021 tcaatttgtc catttcatct gcattcaaag tgaatggtgt agcaagctga ttggataaat
     1081 catcatatac aacttgtcgt ttgggtttgt tttctttgat gaaagctgct actgctgctt
     1141 catacgtttt tttatctgtt ccatttgcca taaaagaagg ccccccttga gtagctattt
     1201 ggcttgtttt aactgtttcg ttaaattgat gatttcaatc gtcaattcaa gctcaagctg
     1261 tttgtttcca gttcgtctgg cttcttgctg catttgtttt tttaatgtta tttcatcagc
     1321 taagccagat ttgctgatca catctagcaa gtcttggatt tctcgtggcg aactttcttc
     1381 tgccaccggt aaatattcga tttccaccac caaccgcctt aaattttcat cttgcaaaaa
     1441 gtctaaaaac tctgcaaggt tgaattcata atgttgtgag acatatgcat cgaataaaaa
     1501 ataaacttct gcatatgcat catgaataaa ctgaaattct cgttctttta aaatattacg
     1561 gacagaaact tctttaaatg cacgatataa tagaatctgc tctgcctttt ctacttgcgt
     1621 caaaggtctc tttttccgtg cagtttcttg tctcgtaacc tgagtgtgtt gactttcctg
     1681 aggttcttga cgattttgct gatttgtcct tcgaagatcg cgcagttgtt tttgtatcgt
     1741 ctctctgccg atctgaaact ctgttgacag ttgagataga tacatatctt gttcgattgg
     1801 tgaaacaact cggctaagtt caactagtaa ttcttcgagg tattcgatct tgtctttttc
     1861 attatctaaa tttttccctt gttttaaata acgtgattta aaagtaaaga ctgtttcgcg
     1921 cccatgagcc actaattctt ggaaagatgc ttctccatac ttacggagat attcatcagg
     1981 atcaagcttt tctggaacga ctacgacaga aagtgctaat cgactgtttt gccgaagcaa
     2041 ttcaatccct cgattggttg cttcaaaacc tgcgttgtct ccgtcatagc aaaagaccaa
     2101 ttctttcgtc agcttttcca tttgttggat ctgttgagct gttagacttg tccccataga
     2161 agctaatcca gttttgatac cagcctgcca tgcagcaatc acatccataa acccttcgaa
     2221 caacaaaatt tctccgtttt tccggatatc gcttcttgct ttatcaagat taaataaaac
     2281 ttgtctttta ttgaacagtt ctgtttctgg actattcaag tatttgggct gatctttttc
     2341 ctgccctttt tcaggtgcaa gccagcgtcc tgaaaaaccg atcgtctggc cttgagaatt
     2401 acgaagtgga aacatcactc tttgataaaa acgatcagac atacggccat catcatgcgt
     2461 gacaaacaat ccagagcgtt ctaacaattc atttgaaatt gattcattct tgaatacttg
     2521 ctgaagaaaa gaacgctcat taggtgcaaa acctatgttg aattcttcaa ttaattctaa
     2581 agacaaccca cgactttgca aatattctaa agctggctgt ccagcttttg tatgcaatag
     2641 catgtgatga tagatttccg ctgctttttc atgagcagca atcagtttcc ccgtagcaga
     2701 ttcaggattt tggacagcta tagtacgctg acgccattga tcttctaacg gtatctgttc
     2761 gaagtctgcc acttttacca ctgcttctgg aaaacttaga ccttctagtt cttgaagaaa
     2821 agtaaacacg tttccgcctt taccacaacc aaagcaatga aaaatctgct tgtcttctgc
     2881 aacagaaaaa gacggtgtac gttcttcatg aaaaggacac agccccaagt aatttttacc
     2941 agactttttc aactggacat attgtccaat cacctcaacg atattcgttt gactgcgtac
     3001 tgtttcgata acctcttgag gaatccgttg agccattttt tcaccccctg ctctattgat
     3061 atcttttggg tttaacagaa gatatttcag gagaataacc ggtaaaaaat cgcactaact
     3121 gaaatcagtg cgattttaaa ggacacaatt atacatgttt gattgtatca tatttcattg
     3181 ctttttcaag tataacacat ttctatctca ttaagcatca atttgctact cttttcagta
     3241 actgattttg aatatcagcc ttccagtaca atactcgaag aacaatattt acgagcatga
     3301 atacaaagaa gtatcctacg ctctctccca accctaagtt tacttccaaa attccatgga
     3361 acagcattgc agctacatat atccctacga tcgtcgttac tgctaggaag atgcgcaatg
     3421 ggttgaaggg ataacaagct tttacaactg ccatgcaact gatcccaatc agcaagtaat
     3481 acatcaaagt agtagtttca gcatttcccc atgcgaatgt ttttccaagt agatacaccg
     3541 caatgatatt cactactaca agcaaggcat ttggtaaagc attaataagt gctgtaggca
     3601 aataacggta agtgattttt cttttatctt gttcaaaaga taaaaagaag gaaggatacc
     3661 cttcaatcgc taaatcaatc agcgtgattt ggatagggat aaacggaaat ccaattgctg
     3721 taacggcaca tataatcgat aaaataaatg aataaatcgt tttgataaaa aagatgcctg
     3781 agactttcgt cacattgttg accactcggc gtccttcaaa caggacttct ggcaatgtag
     3841 taaaatcaga gtccaacaat accagattcg caatctgacg tgttgctcca tcaccttctg
     3901 ccatagcaat gctgcaatct gcttctctta acgccaaaac atcattcaca ccgtcgcctg
     3961 tcattgcaac cgttctgccg ctttctttca attcatttac cagcaatttc ttttgctgcg
     4021 gactcactcg tccaaaaacc gtatagcgat gagcagcttc tcgcacttcc atttcttcag
     4081 taatttgaga aagatcgaca tacgcatcat aattcggtaa tcctgcacgt ctagcgatat
     4141 tggagacagt aaaaggattg tctccagaaa tcactttcag atccacacct tcctcatgaa
     4201 gataagctag tgtttcttga gcgttttttc ggattggatc atcgatttca aacagagcaa
     4261 ccggctcaac aaatgctggt ttttcttcgt tcaattgctg atttggagcg atccccaaca
     4321 taagtacgcg atagccattc gcttgggctg tttttagact gtccggaagc cgatcttctt
     4381 ccaccaaacg ttcaggcgct cctacgtaca cgactcccag ttctggaaat tccatcgcgc
     4441 cccatttgcg atcagaagaa aatgcgactt tgtttgtcac atcataccga ttagaaagct
     4501 caaaataatc tctcatagcc cgcatagtga tgttgttatc actgctttct gccagatacg
     4561 tccccataat ttctgggatg gctttctcgt acagcggatg tagtgcttct acccgctgga
     4621 tactcatttt cccttcagta atggtccctg ttttatccaa gcataatgta tcgacatggg
     4681 ccagcgtttc aattgaatac atgtcttgaa caaggattcg tttttttgcc agtttggtta
     4741 cccctgtcgt taatgcaata cttattagca ataccaaacc ctttggaagc atgcctaaca
     4801 aagctgctgc agatgcaata acagaaattt tgatagaagc atcgcggatg aacagtgctt
     4861 ccaaaaatag tatgatcccc aaaggaataa tcacgaaact agtgaatttg gatactttgc
     4921 gaatcgaatc tacaagttca gaacgaatag ctttgtgagt ctttgcctcg ctagtcaagc
     4981 ggacagcata attatctgca cctactcgga taacttcagc catgacagaa ccgcttgtca
     5041 aaaagcttcc agaaagcaat tcagctccag tttctttgac aatcaaatca gattcgcctg
     5101 tcaaaagagc ttcatttgct tctgcccgtc cagatataac ctgtaaatcg gctggtatct
     5161 gttctcctgc tccgattttt accaaatctc ctaataccag ctctttagta gaaatcgatt
     5221 gttctttacc gtcccggatc actagtactt ctgattggga aacaatcgat aatttccgta
     5281 caagatttct tgcatgtatt tcttgataaa ttccaataac gatattgacg acaataattg
     5341 ctaaatacgc catgttggaa tacgcaccta caaataacag acaaatccca ataccaaagt
     5401 tcagaaaatt gaataacgtc aatatattat cgctgaatat ttgttttgta ctttttgatg
     5461 catcttcctg gtagtcattt tgctggccag aagccatttt ctgaactacc tcttgctgtg
     5521 taagtccatt cattttcttt ttctccattt tccgccaact ttcaaaaata atcacgtgtt
     5581 taagtctagc gtgttccaag attaaaaaca acttgttttt ataaaattta tagaaaaaca
     5641 cacgaatgat tttacaaaaa agatgccaac ctttttgttt gaatcacccg tgtgctgttt
     5701 ttattatcaa tctatcttta cttatattgt tcctccaaac ggctcatcca ccaacctagt
     5761 gctgtaccgg ctataaaaag aacaggacaa gctagtaccc cagcaactcc caatcccgcg
     5821 taatcggtag gaatgatcat aatcgttgaa gcaaaaacta cacctaaaat gaaatggaat
     5881 agcttgctat atgcctttga gaaaatgtaa tccatgattt ttgacaatcc aagaacacaa
     5941 agaatcccac caattccgat cggcacaatc acttcaagtc gtacttcttt aaatccgtca
     6001 gccatcgctt tatacaatcc catatacacc aaaaaattag aagggctcaa tcctgggacg
     6061 atcataccca aaccaatcaa tccgccggca atcagccatg ttccaaaatt ctgatcgact
     6121 tgcccgtcaa ataattgagc gccgtataat aaaaacaaat aagccagaac aaaagtaaca
     6181 accatgatga tcacatcaga agtatctctt ccttttttcc ctgcttcttt ccataacgca
     6241 ggaacagttc cagcgataca tccgacaaaa aaccataata tcgttgtttc atacgcacct
     6301 aataaaaaac tgactaaaaa agaaagtaga aagatgcctg tcactccgcc tagaccaaca
     6361 ggcagaaaaa acaaaacatt ttctttgaaa ttttttgtaa ggtgggctaa aaaagaaatc
     6421 agccgctcat acatgccaaa gacagctgct aatgcaccac cgctgactcc tggcaagata
     6481 aatcctgaac cgataaacat tcccttcaca aaccgtaaca tccagtcttt ggttgtgtac
     6541 cccacttctt gattttccat tttttgcctt ttttccacta tttgcgcact ccttctttca
     6601 tttaatttta ttcatgattg aacatttcct gctattttac ttaaagactt tttcataggt
     6661 ctctattatt ttttcattca ttttgacaat atcaggataa aacatgttac ctccattatg
     6721 atagagatag ccgccattgt aaagcaagga ctggatccgc caatatcggt attgtttccc
     6781 ttcctcattt cctaaaagtg ggctcagcac atctttagaa tacgcttctg ctaattctac
     6841 tgtgttttct ccgccatgat tttttacata ggcaatataa tctttcccaa aattatatgc
     6901 ttggacagct gtccataaat cgcatccttg tttctttgct tctactatca tttccgccaa
     6961 atatttgaca ccttgatcaa tactgtcagc tggattatca atgatctcag cttctccatg
     7021 cagactttcc gagctctgca tgagatctgt cccttttcct tttgattcgg tcaatattat
     7081 cgccttgatc aaattctcgt agttttccat atgataattt tttgcagctg ttgttatttc
     7141 tatttgatag ctttccactc gttttaccga acgataagtt cttcctcata aaaacaacac
     7201 cgcaccaata accgtcagaa tgatcatgta aaaaagaata cggataatag ataatcgttt
     7261 tcgcttcatt tcatcaccta gttattttga catctttttt tatcttacta tttctttatc
     7321 ttcttggcta tttcttcctt gatttctgtg tacgccttaa gaaaaaatga attttcctgt
     7381 attttttgac atcttacgaa aatgatcgtt gtttttcagt aaaaacaatt gcccaatgac
     7441 tgatataatc gttatacttt acacagtagg tgattatgtg aaaaaagaaa agaaatatgc
     7501 aaaaatgcca gatgggagcg aaatttacta tgaaaagagc ggtcaaggct tccctctctt
     7561 tttactgcat ggcaatgacg gcagcggccg ctttttttca gaacaggtcc ctgtactcga
     7621 acgctactat accgtgtatc tagtagatag ccgaggacat ggtcgttcga caaacgaagc
     7681 atctatgttg aattttcagt taatggccga ggatctgaat acgatcatgt tgctggaaaa
     7741 aatcgatcag gcagattttc ttggatttag tgatggagca aatctagcac tggtatttgc
     7801 cagcagcttc cctaaaaaag ttcatcgatt gattttaaat tctggaaaca cgcttgttaa
     7861 aggcgttcgt ttttctgcca gagtcatcag taatattcat tatgcatggg tatggttact
     7921 ctctttattt cgcccaagtc ttcgcaaaaa tcttttagtt atcaaacttt tgctgcatga
     7981 catcgggctt acagaaaatg atttaaaaaa aatcaactcc cccactttga tcatcgtggg
     8041 aaaaaaagat gtaatcaagc taaaacattc tttgtatata gctaaaacta taccaaaagc
     8101 ttcctttgtg cttgtgaaag aacaaggaca tgaattagct agaaaagatc ctgaacgctt
     8161 caatcgagaa gttcttcaat ttctttctga aacataacta ttaggaatag aaggtgattt
     8221 atttgttaaa aaataccata caatggctga aaaaccacct aggactgttc aaaacaatct
     8281 ttttgatttc tgtcattgtc attattgtcg gtgaattgat gtccatcggt aaaactctat
     8341 ctatccagca attaggagaa acgtttgcca ctatacctgt atggaaaagc ggactaatgc
     8401 tattgattgg tcttatctct gtgttaccta tggttggtta tgatattatt ttgaatcgga
     8461 tgcttgaaca aaagcagaat cctcgttacc tgtttgagac aagctggctg atcaatacga
     8521 tcaacaatat tgccggtttc ggcgggtttg ttagtgtggg gcttcgctct gaactatatg
     8581 gacaaaagaa agagagcaga aacgtgatcc aagcattatc gaaagttttc ttgtttttga
     8641 tggctggact ttctatttac agtctgcttt cttttttact tgtagtcttc acgccagttg
     8701 ctcctttttt gaagcaatat tggatatggt tgatcggcgg cggtctatat tttcctgtcg
     8761 tctttttatt tactactttc aaaaagaaag gattccttgg tggtacctca cttaaaactc
     8821 aaggacagct attgctagtt tcattgttgg aatggaccgg tgtcctgttt agtttccttt
     8881 ctgttggtta tttaatggaa atccggattg atctgtggca aacaattcca ttattcattg
     8941 ctgcttcagt gataggaatc gtgtctatga ttccaggaga aattgggagt ttcgatgtaa
     9001 tgatgattat cgggttgtcc gctattggtg tgccaagaga aatagtcgtt gtgtggatct
     9061 tgctctatcg cctgttctac tacatcattc cgtttttgat tggaatcgtc tttttcttca
     9121 aaaatatcgg ttccactttt gatcagcgtt attctggtat ccctaaacaa ttagcaacag
     9181 aaattgcgca taaaatcgtg gttgtcttat tatacttttc tggaatcatg ctagtacttt
     9241 ctgccactat cccgcaagct tttacagagt ttcgctggct tcatagtttg aacccattga
     9301 aatttcattt tattatccaa tttcctagta tactattggg atttctttta atcattatgg
     9361 gcagagggat cgcagcacgt gtgaaacgag catatttgcc tacaatcttc ttgattgttc
     9421 tagctttact ttatgtttta ttaagtgatt ttagtttcac accggtggtt ttcttgtcta
     9481 ttctgcttct gatcattctt gcttctaaaa acgaattatt tcgggaacaa ttgatctatt
     9541 catgggagtg gcgtacgatc gatggaatca ttattggtgc actgtccttg ctttacatcg
     9601 ttattggggt atacaatctg ccggatttcc ctcatcggcg tcaccatttt atttcctttt
     9661 tcttatttcc ttctgaaaag atttggtttt cgggattgtt ggccatcatc gctgttagtt
     9721 tcatgatcgt tctatttgtt catttccttc aaggagaaaa gaaacagatt ggcgaagcct
     9781 ttaatgaaga aaaggccctg aagatcctta caacttatgg aggaaactcc gatagtcaat
     9841 tgatcttctt aaaagataag cggatgtttg cctatgaaaa agacggtgaa ccaactgttc
     9901 ttctccaatt cgcttgcttc aataataaat gtattgtaat gggtgatcct tctggtaaga
     9961 aagaagattt tccagaagca atcgaagctt ttatcgaaga aacagatcga ctatgctatt
    10021 taccagtttt ctacgaaaca agcgaagaga ttgttatgat tttgcatgaa ttcggctacg
    10081 atttcattaa gatgggggaa gaagcttatg tagacctgaa tagttttaca acttcaggga
    10141 aaaaaatgaa gggcactcgt gctgtcttga atcggatcga acgagaagga tttacatttg
    10201 atgtcttaca accaccattt tctactgagc aaatgagtac tttcaaaaat atctctgata
    10261 attggttggg ttcacgaaag gaaaaagggt tttctttagg ctttttctct gaagattatc
    10321 tacaaagagc gcctattgca gttgtcaaaa atacccaaga agaaattgtc gcttttgcaa
    10381 cgatcatgcc tacttacacg aacaataaag taggaacgat tgatctgatg cgatatgatt
    10441 ctaaaaaagc accttctggc agtatggatt tcttgttctt gcatttgttt gcctatatga
    10501 aagaagaaca tatccaatgg ttcaatttag ggatggcccc tctttcaaat gtcggaactt
    10561 ccagaaagag ttttttacag gaaaggattg catacttggt ttacgagttt ggttctcatt
    10621 tttattcttt ccatggatta agagaatata aaagcaagta cgctacaaac tgggtctcac
    10681 gttatactct atattctaga gatagttgga ttgcatatgt tatgattgct ttgctgatta
    10741 ttgataatgc gccagttgag cagacacaag gtagaataag tggtttgaaa aaatggatac
    10801 gaagaaagta ttaaaaactg cataccgata gatgaacact ctcatgatga ataaaagaga
    10861 ctgaagtaaa acgaaagttc atgcttctac aatctcttct tatacaattg aagaacaaag
    10921 agcggtccaa acagaaatcc tgtttggacc gctcttttat tttaaacatt tttcattacc
    10981 aaaatattcg agtcgcgttt cttatgcttc gatctctgta acgatacctg aacctactgt
    11041 ccgtccgcct tcacgaattg agaatgtcgt tccgttttca acagcgattg gatggatcaa
    11101 gtcaacatca atcgtcacgt tgtcaccagg catgaccatt tctgtatctt ctggcaatgt
    11161 gattgttcct gtaacatcag ttgtgcggaa gtagaactgt ggacgatagt tgttgaagaa
    11221 tggagtatga cgtccacctt cttcttttgt caacacgtac acttctgctt tgaattttgt
    11281 atgtggagta atggaacctg gtttagcaag tacttgtcca cgttcgatat cgtcacgttg
    11341 gatcccccgt aacaacacgc ctacgttatc cccagcttcc ccgtaatcta acgttttacg
    11401 gaacatttct acacctgtta caactgcttt ttgtgtttca ggtttgatcc ctacgatctc
    11461 tacctcatca ccgacacgaa cagcaccacg atcaatacga cctgaggcaa cagttcctcg
    11521 acctgtaatt gagaagacgt cttccactgg caatagtaat ggtttatctg tatcacgttc
    11581 tggtgttggg atatattcat ctactgtatc catcaattcc ataatagcag cttcagcatc
    11641 tggatcgcct tgcaatgctt ttaatgcgga acctttgatc acaggagtgt catcgcctgg
    11701 gaaaccatat tcgctcaata actcgcgaac ttccatttct accaaatcga tcaattcttc
    11761 atcatcgact aaatcaactt tgttcaagaa aacaatcagg tattttacac caacttggcg
    11821 tgacaataaa atgtgttcac gtgtttgagg catcggaccg tcagttgcag aaacaactaa
    11881 gattgcacca tccatttgtg ctgcccctgt aatcatgttt ttcacatagt ccgcgtgtcc
    11941 tggtgcatca atatgcgcgt agtggcgttt ttctgtttca tattctacgt gtgcggtatt
    12001 gatcgtaatc ccacgttcac gttcttctgg agctgcatca atactagcat agtcttgagg
    12061 atttgctagg ccttttttgc ctaatactgt agtgatcgca gcagttaaag tagttttccc
    12121 atggtcaacg tgtccaatcg taccaatgtt aacgtgtggt ttacttctgt cataatgttc
    12181 ttttgccata atttacaaac ctcctaaata atatagatca ctgaaaaatt tcagctctca
    12241 cctttttatt atagtctttt tttgactaaa tcaaaaagaa tacgtccgac ttactttttg
    12301 taagattcga actaagctta ttatacgtca atattggtca aagtcaacta agagcactcg
    12361 tctgtcttat acgcgaaaaa agtacctttt ataaataaaa aaatgtgttt tttgagaaaa
    12421 acttcaagat agtccttaac tttcttttga agcaataaga acaaaatgag gaaaagttta
    12481 aaattcagct gttgccaaca actttcaata gacgaaatct tctatttcgt taataataag
    12541 gaaagaggtg aagtacatga aaaaattaat tggcgtactt gtcgttttag gcatatttct
    12601 cggcggaagt tttgcagctt atcattattt ttacggcggc gaagcttatt atacaaaaat
    12661 cacgacgaat ggcgaaatgt cagctaccca agcagataac ggagagaaat tcacgactta
    12721 tacctacaag caagcggctt ataataaaga tggtgaaaaa aaacaggtga cgcttcgcga
    12781 agaaagagaa catccattaa agatgaatgc gtatcttaaa ctcaaagtca atcctcgtaa
    12841 aggtgtatta agctgggaag aaataaaggc ttctgaagtg cctaaaaatg cggcagaaaa
    12901 aatcgataat tagtaaatgg tcttaaaatt ttattagata aaa
//
LOCUS       DAZULY010000067        12898 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000067 DAZULY010000000
VERSION     DAZULY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.068"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACG81A_001222"
     CDS             133..3660
                     /locus_tag="ACG81A_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFS5014595.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACG81A_001223"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACG81A_001223"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFS5014596.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACG81A_001224"
     CDS             7448..7789
                     /locus_tag="ACG81A_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS5014597.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACG81A_001225"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACG81A_001225"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFS5014598.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACG81A_001226"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACG81A_001226"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFS5014599.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG81A_001227"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG81A_001227"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFS5014600.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4380 a         2241 c         2737 g         3540 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaac
//
LOCUS       DAZULY010000068        12769 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000068 DAZULY010000000
VERSION     DAZULY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.069"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1120)
                     /locus_tag="ACG81A_001228"
     CDS             complement(<1..1120)
                     /locus_tag="ACG81A_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5014601.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEI"
     gene            complement(1242..1838)
                     /locus_tag="ACG81A_001229"
     CDS             complement(1242..1838)
                     /locus_tag="ACG81A_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5014602.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(2038..4680)
                     /gene="alaS"
                     /locus_tag="ACG81A_001230"
     CDS             complement(2038..4680)
                     /gene="alaS"
                     /locus_tag="ACG81A_001230"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFS5014603.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            5068..5499
                     /locus_tag="ACG81A_001231"
     CDS             5068..5499
                     /locus_tag="ACG81A_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5014604.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(5544..6893)
                     /locus_tag="ACG81A_001232"
     CDS             complement(5544..6893)
                     /locus_tag="ACG81A_001232"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS5014605.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(6963..8126)
                     /locus_tag="ACG81A_001233"
     CDS             complement(6963..8126)
                     /locus_tag="ACG81A_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS5014606.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            8255..8578
                     /locus_tag="ACG81A_001234"
     CDS             8255..8578
                     /locus_tag="ACG81A_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014607.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(8761..10110)
                     /locus_tag="ACG81A_001235"
     CDS             complement(8761..10110)
                     /locus_tag="ACG81A_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5014608.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(10179..11132)
                     /locus_tag="ACG81A_001236"
     CDS             complement(10179..11132)
                     /locus_tag="ACG81A_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFS5014609.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(11234..12424)
                     /locus_tag="ACG81A_001237"
     CDS             complement(11234..12424)
                     /locus_tag="ACG81A_001237"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFS5014610.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         3665 a         2695 c         2210 g         4199 t
ORIGIN      
        1 taatttccaa ttggtaatct ccgatttttt cttctatttg actacgtaag attctttcta
       61 gttctgtatc aacaaactgg aatttctcag ggaccactac atcaaattgc agcgtttttc
      121 cttgatgaat tactctaaaa tcatgaatat taagccctgt acctgtttca agaagaattt
      181 gctttaactg ccgcaagata ttatagtatt tttcgttatc aacaggcatt ggatccaagt
      241 gacagacaag ttccacatcc aaagactttt taaagtcttt ttctatcaga tcaataatct
      301 tatgagccac attcaaattc attgattcat ctacttcgat gtggactgat gcaaagcgtt
      361 tattgggtcc atagttatgc accaataaat catgatagcc aagtatatca tcgcaagcat
      421 ttaaatgctc ttccatttct tcaatctctg ctggcgttgg gcgacttcct aatagttcgt
      481 atacaaaatt tcgaagcatc gtcaatccac tataaaggat atataatgct aaagcaaaac
      541 ctatatatcc atcaatacgc catccagtta cccattcgat tgctgctgaa agcaatacag
      601 ccagtgtagt aaagacatca ttataactat ctgttgctgt agctctcaat gtttcagaat
      661 caatggattg cgcaataacc tgatacattt tgccttgtgc aaatttcaaa aagatggaaa
      721 tgattaggac aaaaaagacg atgggtgtta aagagacatt ttctggatgg atgatttttt
      781 cagcagaaga ctctaaaaat tgaaatccta cataagtaat gatgatcgaa acaaataacc
      841 cactgatata ttcgaagcgt tcgtgtccat aaggatgttc ctgatctgct ggctttgcag
      901 caattttaaa tccgatcaaa gtcaatatgg atgaagcagt atctgataga ctgttcactg
      961 catcagccat aatggagaca cttcctgaaa aaagcccgat cattagctta gagacaaaca
     1021 agatcagatt cgtaaccaat cctaagatac ccgctagtac tccttttttt gtccgctgtt
     1081 gcggtatttg tttctttttt tcttcttgtt tcttagccaa gttttttacc ccttctagtt
     1141 tgcttgttgc tcaggtaaaa agatggagag gacgattgtt ttcctctcct ctcttccttt
     1201 ttctatctat cgtgcgtcct attactatta aaagatggtc attattctgg tttcaacggt
     1261 gttgctgcaa ataacgcttt tgctagtaat ggtgttatga ttcccgcacc tattgcttct
     1321 ggtatcccat tgattgctgc aacccctgct aaggttttaa ataaaagagc agagtctaca
     1381 ccataggcat ctgccgttgc acctgtataa aataatccca tgaatcctaa cactaagact
     1441 gtattgacca atgaacccag tacccctgca aaggctgagc tgatcgttat tgatttattt
     1501 ttttgataaa tccagtggaa aaccaagcct gctactaagc caacaagtac tctgggaact
     1561 accgagataa tcggattcgt gaacactaat gtatcaaatg gcgttgttgg catggcatag
     1621 gcacgaacaa ttgtagcgat tccccaaaac aagccaatca ccatgccatc ttttgggcct
     1681 aatactactg cagcaataat tactgtgata tggataattg ttaaactaat gaatcctaat
     1741 ggaataaacc ccagccaagg aaccatcgct tgtacgatga tgattgctaa caagatcgca
     1801 cgtaagacta gtcgaaaggt cttatttcta ctattcattt tttcacccct ttttatttat
     1861 actaatcgta aaaatttctg tcactttcgt caactaaaga ccatccgaat ccgaccattt
     1921 gattagatgc gccatccaat tgaaaaaagt atgagggcaa gacttgtttg tcttcgcccc
     1981 cataatcatg tttcgttcaa tgtttgaact tttgtttttc tgtcaaacgt aaacgcccta
     2041 ttgttgcgca acccattctt tcgcagcttt aagtgcatct ggaataccag caggattttt
     2101 cccgccagct tgtgccatgt ctggacgtcc accaccgcca ccgcctactt tcggtgcgat
     2161 tgctttaatc aagtcgccag cttttagtcc agcttcattt gattctttcg tcattgccgc
     2221 tagcagactg acttttcctt cttgttcgtt tgctaaaaca agaatatctg aaatattttt
     2281 ttgtttccat tggtcagcta attgacgcag ctgattcata tctttcactt ttacttgtgc
     2341 tgcaatgatt gtttttccat tgatcgtttc tggatctttg aatacatcac cagcttgttg
     2401 attcgctaac ttgctagcta attgttcatt ttccttttgt agctctctta cttgctgttg
     2461 caattgttcc gcttttgaaa caacttcttt caattgaggt gatttaacaa ttgttgctac
     2521 ttgttttagt tgtttttctt cttcttcaag tagttcgtac gcttctttac ttgttacagc
     2581 ctcgattcgt cgcacgcctg caccaattcc tgattcagaa acgattttga agattccgat
     2641 gttttctgta tttgctacat gtgtgcctcc gcacagttcg attgaccagc cgccaatatt
     2701 tactactcga acgtcatgtc cgtatttttc accaaacaaa gccattgcac ccatgttttt
     2761 ggctgtatcg atatctgttt caattgtttc aactgggata gcttcccaga ttttttcgtt
     2821 gacgattctt tccatttcag ctaattcatc tgaagtgatt tgaccaaagt gggtaaagtc
     2881 gaatcgtaaa tgtccaggag cgactaaaga accagcttga tttgcatgct cgccaagtac
     2941 ttctttcaat gccttgtgca gtagatgggt tgctgtatgg tttttgatga tccgattgcg
     3001 catcgattga tcgatcaata gatgataggt ttgtccttca ctcattggtg cttttacttc
     3061 tacggtgtgc ataaattgac cattaggcgc tttttgaaca tctgtaacaa ctgctacgat
     3121 ttcgcctact tcattcatga tccatccttg atccgctact tgcccaccca tttctgcata
     3181 gaaaggtgtt tgatcaaaga tcatttgagc ttcaccttct gcgacttcat tcaaaagaga
     3241 ctcaccttta acgatgacca ataattgact atctgattct tgttctgtgt atccaatgaa
     3301 tttactttct actttgatat ctgtcaataa agcagattgt acccccattg atttttcagt
     3361 actgcgggca gagcgagcac gttcgcgttg tgcatccatt tctttttcaa aaccagcatg
     3421 atcaactttc agtccttcat cttctgccac ctcttccgtt aattctaccg ggaagccata
     3481 tgtgtcgtat agtttgaaga tgtcttttcc tgctagagtc gtcccgtttt cgttttttac
     3541 ttcttggatc acttgattta agatttccaa accttcattg attgtttcat tgaaacgttc
     3601 ttcttctgta cggataactt tttcaataaa ttctttttgt tgaagtactt caggataata
     3661 acttaccatg atttccccaa caacagggac taaacgatat aagaatgctt gttcaatacc
     3721 taattttttc ccatgcatga cagcacgacg aagcaaacgg cgtaagacat accctctccc
     3781 ttcattcgat ggcaatgcac cgtcgccaat agcaaatgaa agggcgcgaa tatgatctgc
     3841 gattactttg taagaaatat ctgttactgg gttttcacca tattttactt gtttgcttag
     3901 cttttctacg gcgtggataa taggcataaa caagtctgtc tcaaagttcg ttgacgcatc
     3961 ttgaacgatt gagaccatgc gttccaatcc catccccgta tcaatgtttt tatgaggaag
     4021 tggttcaaat gtgttatcag gcttatggtt gaattctgaa aatactaagt tccagatttc
     4081 taaataacgt tcattttctc ctcctggata gttttcagga tcgtcttctg ctaaatcgtt
     4141 ataagcttct ccacggtcat agaaaatttc cgtatccgga ccacatgggc ctgcaccgat
     4201 atcccagaag ttgtcttcta catcaataat gtgatctagt ggcaggccaa cttcttcatg
     4261 ccagatgcgt tttgcttctg tatcttttgg ataaactgtt acataaagtt tttctggatc
     4321 gaatgccatc cattttggat cagtcaaaaa ttcccatgcc cagtggatgg cttcattttt
     4381 gaaatagtca ccaatcgaaa aattacccag catctcaaac atcgtatggt gccgtgctgt
     4441 tttaccgaca ttttcgatat catttgttcg aatagatttt tgggcatttg tgattcttgg
     4501 attttctggc acaactgaac catcaaaata ttttttcaat gttgcaacac cagagttgat
     4561 ccacaataag gttggatcgt tcacggggat caaagacgca ctaggttcaa tcgtatgtcc
     4621 tttagattgg aagaaatcaa gaaacatttg tcgaacttgt gcactactta attgtttcat
     4681 gtcattacct gctttctata aattaaatac aaaaaaacac catcacagcc aaggacgaaa
     4741 actcgcggta ccaccttgat tgcaatgatg tgtaatcatt acctcttaaa ctcgataacg
     4801 ctgagctgcg tcggtatttc taccaaaata gaaaaaggga gcaatttgat tagtgactat
     4861 tcttttttca gccaagaaag aattttctgt gagtcttaaa aaacaaatca tatccttttc
     4921 gtacttaatt agtatacgca ttctattgat cactgtcaat atctcaattt ttattttcat
     4981 ttccttttgg tcatactcca cgatttttgg tacgataaga ataaaggaat caatacatct
     5041 ctgctattca tgacaaggag gaatatgatg aaaaaaatag ttggagcgga tgaatggata
     5101 cgcgcagcta gctgcttttt gagatatcaa gtttttgtat tagaacaagg gattcctgca
     5161 gaactagaat tcgacggaaa agatatcccg caacaaacct attatctatt tcttgatcaa
     5221 aaaagaccag tagctacttt gcgttatgaa aagaaaacac ctacttgctt caagccggaa
     5281 cgattttgcg tagcaaaaga ataccgagga aaagggttag gcggtcagtt gcttgaagct
     5341 gctgaaaaac gggcggtttc agaaggttgt cagactagtt atgtagtagc tgaaatgacg
     5401 gctgtagatt tttatgaaaa acaagggtat catacttgca cagaaccttt tattgaagat
     5461 gaagtacttt gtatcggaat gaaaaaaagt ctagcttaaa aacaagagat tgtgacacgc
     5521 cttttgtcac aacctcttgt tttttattta tgtgaatttt tcttattttt acgagcagta
     5581 cggctttgct gtctgcgttc gattttgcgt tcttttttat ttttttctgc aattgcccaa
     5641 tcgatttttt tcttataacc aggcttgatc tttttctttt tcttcttcac aagaccaatc
     5701 agcgtgggat ctaatacatc ttttgatttt tctcgtttgg cacgtctatt acgatcgtat
     5761 gttttgacga tttccccgtt tttgatttct tttggttcaa atgagacgcc caatttttct
     5821 atcgcatcaa tggcttgctc atcatttggc gaatataacg taatggccgt tccttttaag
     5881 ccattacgtc cagtacgtcc gacacgatgg ataaagaagt ctagatcttc tggaatttct
     5941 gcattaataa catgagagac accttcgata tcgatccctc tagcagctaa atcagtcgct
     6001 acaacatatt gatactccag attctgtact tgtcgcatca ctcgttttct ctctcttggg
     6061 gtgatgtctc catgaatctt tgctactttc aagccttggt ttttcaagta atcggcaatt
     6121 tcatccacat gctgttttgt attggcaaat acgattgcta aataaggatg accaattgtc
     6181 agcagttgat aaatcatttg attctcatct ttaccttttg ttgaaatcag ccaattttca
     6241 atgtcttcag aaataactgc ttttggtttg atttcttcaa tgattggatt ttccatgtat
     6301 tttcgtaaga atggacgaag tttttctgga atagtggcag aaaaaactaa aaactgcagc
     6361 ttttctggta atcgacttgc aatttggtct acttcattta aaaaacccat atccaatgtc
     6421 atatccgctt catcgacaac aaatgcaaat gcggtgtgaa tcttcagcgc ttgttcattc
     6481 atcatgtcta atattctacc aggcgtgcct actacaacct gtggctgttg gtgatgcaga
     6541 cgatttaatt gacgctgttt gtctgtaccg ccgacaaaat tcgagacacg gatctccggt
     6601 gtagaaaatt tagcgatttg tgtagctgct tcatagatct gtgcagctag ttccctactt
     6661 ggagctgtaa taacaacttg tacttcctct ttttctgcat tgattttttc aaacaaaggc
     6721 aacaaaaacg tatgtgtttt tcctgtacca gtttgagatt gcccaatgat attttttcct
     6781 tttgcaatta aaggaatcaa acgttcctgg acctctgtgg gctgtttgaa gccctttgcg
     6841 gttaacgctt cattgataaa cggtttgaag cggaatatat caaaattatt cattcgtgca
     6901 cctcttctct ttaaattatc acgactttgg cattataaca cagacaaagc gtttttgctc
     6961 agctaatttc gagaatcatc atcctttgtt tggccaatcg tgctaacagt atatgtgtga
     7021 ctagcttttt cataaaacag aatccgtttt tttctcaaaa atgatagcaa gatatttaaa
     7081 ataaaaatac cttgtagtaa caagacaccg agagaaatga atactgcact catctgcaac
     7141 accgtattcg ttgtcgaatt catgatttgc tgaagcaact gccccaacca gcttaaacca
     7201 taaaaaagaa agtacagata cccgtagcgg atcatcaatg cttttaaaga aattcgttcg
     7261 cggtcttctt ccaccaaacg gatacgcaca atttttttgc caagtgtcca tccattggtt
     7321 ataaacggta caatcataaa gtatacccct acttctaata aaagccacca gatatgagaa
     7381 gtaactgccc ctgaatcgaa cgcaatttta aagaaaatcg ccgctaccaa attaataaga
     7441 gataaaaatg tccagtcgat caaataagcg accaagcgtc gagtcaatga gacagtcttt
     7501 cctttttcat aagaaagttc atccattcgt cttcttgtag gaagcataaa tgtaaataac
     7561 ggcgtaacga caaaaccaat gattccaccc aatgtatttg tcactaaatc attcacatca
     7621 aataagcggt atggtcttgg gtagataaaa taaagtccac ttagctgcgt caattcaaaa
     7681 aatagagaaa ggcagaaact agcaatcatc gttttcataa aagaaagccg gaaataatat
     7741 cttaaataaa tgccaaatgg tatgagtaat aaaacattaa aagctggttc caaaaataca
     7801 ctctgcttca ttgccggcag gtaggtacta ggttcagaca agaccaatac cgtgttgtct
     7861 ctgaaatgtt gccatgaagc aaataaatgc agctccatct ttggccctgt catttgggca
     7921 accgtctcct ttgccggaag tggtaagatc accaaaaaat acgcacacaa taaataaaaa
     7981 acgaaagaat acaaaataac tgcccttgtt actagaaatg ttccatactt tcgatattca
     8041 taaataaaaa aaacagatga gatcgctaag gctaaaaatg gaaaaacaat cagcgcaaaa
     8101 cgaatcggtt cgatataact tgccatatga ctcctccttg attctttgta tctctcttat
     8161 cttagcagtc ctttgctgtt tcaagcaagt aacgaggcta ttatttctca tttctttcaa
     8221 agtaaagtaa gataaacaca aagggggtgt cgagatgctg aatatgtact ttgtttttgg
     8281 cgtacctatt tttttactgt tcctttatgc tacgatcgct tatgtaagaa aaagaactac
     8341 aattcattat ttaggattta ttctcctgat tatttcgggt ttcatgctag tcttcaatct
     8401 tcagacttgg cagcaagcac ttcttgaaat ggataaaatg actccacatg ctttgtctaa
     8461 agtgcttggc taccctgtct atctgatttg gcttcccatt ttcatttctg gttgcttggt
     8521 tttattaaat atttatcgcg gcgtacgccg aatagttcag ttacgcaaat caaagtaaaa
     8581 gacagaaatc ggttgagccc gatttctgtc ttttactttg ctatttcatc gtagacaaaa
     8641 cgacatttac gtgatcaaaa ttaacgagta cttttgacat ttcttccacg tactcctcat
     8701 ttaaataaaa tattttgttt ggcagagaag tgcagttcta ataaaattcg aatgttttag
     8761 ctatctacgt tttaaataga gataaatcgg gataccagca agtgtcaata ctatccccat
     8821 cgaagctaaa aaggtctgag tgataagcgt gcttgatacg ataaaagtcc ctccaatgat
     8881 tgctactagt ggaataaacg gatacattgg aactttgtat ggtcgaaaca aatcaggttc
     8941 ttttttacga agaagaatga caccaacaaa gaccatcgta taaaaaatcc aaatgacaaa
     9001 gatcagcata tccgttagtg tatcaaaacc accgatcatc atcatgccaa tagcgatgac
     9061 tagctctaag atacctgcgg ctacaggaac tttagtttta tcatgaagtt tagcaaagag
     9121 cttgctaaaa ggcaggttgt tttcttttgc cataacataa gggagacgca ttccagtaag
     9181 ggtatagcca ttgatggttc cataaacaga aatcaagata ccaactgtga ccagtcttcc
     9241 accgaagccg ccaaaaatca tttttgcaac gtcacttgcc gcattgctgt ttcctgcgac
     9301 tccgtcgata gatgcggttc ttaagaacac cgcatttact aataaataaa cgatcatgat
     9361 tccgatgatt cctaaagaaa ttgcttttgg caaatctttc gccggctttt tcaattctcc
     9421 cgaaatattt cctacgtgta tccaaccgtc gtatgcgaac atcgttgcta gcaagcccgc
     9481 tcccaaggct gagaaaaatg aaagattttc tcctgcttgg atcggaaaca gttggaagtc
     9541 tactccttct tgtcggaaca aaccgaaaat gacgatcaca aacaaaggaa taagcttgca
     9601 gacaagcgta atcgactgaa aagcccctcc agctttagaa cctaaaaaat tgatcaacag
     9661 gatcgataca gcagctgtaa cagctacggg aacaatcatt gactgggaaa gaccaaatag
     9721 attgacgaac tgtgtaccga aaatgatcga caaggctgct acatttgctg gaaaataaat
     9781 cacgacttgc gcccagccca ataaaaaagc tgctgtattt ccgtaaatcc gttcgatata
     9841 tttaatcatc cctcctgttt ctggaatagc agctgccagt tcagctcctg ttagtcctgc
     9901 acagacagaa atcataccac cgagaaacca agaaaacata tgcaaactgg cggatcctgt
     9961 aacttctgct acgcttgcgg ctttaaaaaa tacccctgca ccgatcactg tgcccataac
    10021 agtcgataaa gcagtaaaaa atcccatcgt acgttttaat tcattttcta tttttacttc
    10081 ttttactggc gtggcttgat attcttccat cccttgtccc ctttcatacc atagccaatc
    10141 ccaaaagtat aaaaaacttt ttggatgtgg ttcttgtttt ataccgtctc taccaatttt
    10201 tctttcagtt tttccaacat atcctttgtc atcacatcta agtcgtattt cggcgaaaag
    10261 ccccattcct cttgcgcaca agaaacatct agtgaatttg gccaagattc tgctattgac
    10321 tgacgtacag gatccacatc ataatccatt tcaaaatctg gtatgatttt ttgaatagat
    10381 gctttgatct cttccgggtc aaaagacatc gccgatatat tgaaggcatt tcgatgaacc
    10441 aatcgatcag gatctgcatt catgagtttt ataatggcat cgatcgcatc tggcatatac
    10501 atcatatcca ttgatgtacc ttgatcaatg aaactagtgt actttccttc tttgattgct
    10561 gaataataaa tatcgactgc gtagtctgtc gtaccgcctc ctggcaaggt tgcataagaa
    10621 atcaatccag ggaaacggac accacgagta tctacgccat atttcacata ataataatca
    10681 catagtagtt ctccagctac cttcgttacc ccatacatgg ttgtaggacg ctgaagagta
    10741 tcttgtgggg tatgatcttt tggagtattt ggaccaaacg cgccgattga acttggcgta
    10801 aaaaatttca gctggaattc tctagcgact tccaatgcat tgaccagccc agtcacgttc
    10861 aaattccagg ctaattgtgg ttttgcttct gcaacagctg acaaaagagc agccaaatga
    10921 atcaatgtgt ccacttgata ttgttctgct atgctgcgca ttttttcgta atccattaca
    10981 tctaggatct caaagatatc gtttttctca atgacttcag gcatgcgtat atctgtcgca
    11041 atgacatttt cactgcctag ttcttgacgt agccgtgtga tcaactctga accaatttgt
    11101 cctaaacatc ctgtcaccaa tacttttttc atgatgatcc tccctgatct tttttactac
    11161 tatcttcaga tgattaacaa tatatgcgtg aacatgttct ctaattttaa atagacgaaa
    11221 aaactaaatt tagttaaata atttccattt cttttcctat tttttgataa actgctactg
    11281 cttcatcaag catttccctc gtatgttccg cagtcggcat atttctgatc ctgcctgtcc
    11341 caagaggcac agttggataa acgattggtt tagcataaat cccatgttcg atcagcttct
    11401 tagagaaagt ctgagtcact ttttcgtctc ccaaaatgac tggagtaata ggtgtttcag
    11461 acataccgat gttatagcct acttttttca attcttcttt aaaataatta gcattttccc
    11521 aaactttttc gacaagttcc ggatgttctt gcatcaaagt aatcgaagcc aatgcagctg
    11581 cagctgcacc tggagttagc gaagtagaaa ataggaatgg ccgacttcta gctttcaacc
    11641 agtcgattag tgttttcgat ccagctacat aacccccaac aacacctata gctttcgata
    11701 acgtgcccat ttgcatatcg attttgtcgc ttaatccaaa atgcttgacg gttcctgcac
    11761 cttttccagt tacgcctgag ccgtgcgcat catcgacgta ggtgatcagt ccatattttt
    11821 caacaatcgg aataatttcc ggcaatcgtg caatgtctcc atccatggag aaaacaccat
    11881 ctgtgatata catgattttt ttatatttct tactttctat cgcttccttt gcttttttct
    11941 caaggtcttt catatcttga tgtttaattc ggatgatttt tgctccggat aagcggcaac
    12001 cgtcaataat agatgcatgg tttaattcat ccgataagat agcatcttct tttgtcatta
    12061 ctgcagagat agcgcccata ttgcagttga atccagattg aaaggcgatt gctgcttctg
    12121 tccctttgaa ttctgcgatt ttttcttcta attgctgatg gattttcagc gaaccattga
    12181 tcgttcggac tgctcctgct cctacaccat aagtttcggt tgcttcaata catgcttttt
    12241 tcaattcttc gcgattggca aatccaagat aattgttgga tgctagattg atcatctttt
    12301 ttccgtctac gatgatacaa gcaccatttt cactttctag tatgtcgatc gtattgtata
    12361 accccttttc tttcagttga tccaattctt tctctaaata cattgttagt tctttggaag
    12421 acattgtctc cacctccata ttcatctgtc ttgcagaaca taccaaacat atatcgtgtc
    12481 attcatctac aaagcaagta tgctatattt ttgataacgc ttacactgat tatgagaatt
    12541 atttaaccac attttttatt tacagtatct ttttactttc aaatgactta tatagtatga
    12601 acaattacct tttagataat gatctatttt actctacctg ggcagataaa tacaacacaa
    12661 ccattgatag aagccctttc gttaaaatca attgtattct gaatatttaa tttatttatt
    12721 gcatagacca ataaaaatac ataaaatttc catcttcgtt tatcagcaa
//
LOCUS       DAZULY010000069        12734 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000069 DAZULY010000000
VERSION     DAZULY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.070"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACG81A_001238"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACG81A_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS5014611.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ACG81A_001239"
     CDS             complement(322..891)
                     /locus_tag="ACG81A_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFS5014612.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ACG81A_001240"
     CDS             1077..1979
                     /locus_tag="ACG81A_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS5014613.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACG81A_001241"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACG81A_001241"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFS5014614.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACG81A_001242"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACG81A_001242"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFS5014615.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACG81A_001243"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACG81A_001243"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFS5014616.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ACG81A_001244"
     CDS             8547..8732
                     /locus_tag="ACG81A_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFS5014617.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ACG81A_001245"
     CDS             complement(8794..9990)
                     /locus_tag="ACG81A_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS5014618.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ACG81A_001246"
     CDS             complement(10160..11596)
                     /locus_tag="ACG81A_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFS5014619.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="ACG81A_001247"
     CDS             11889..12698
                     /locus_tag="ACG81A_001247"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5014620.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DAZULY010000070        12642 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000070 DAZULY010000000
VERSION     DAZULY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.071"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..1495
                     /locus_tag="ACG81A_001248"
     CDS             68..1495
                     /locus_tag="ACG81A_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5014621.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1587..1952)
                     /locus_tag="ACG81A_001249"
     CDS             complement(1587..1952)
                     /locus_tag="ACG81A_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS5014622.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1949..2314)
                     /locus_tag="ACG81A_001250"
     CDS             complement(1949..2314)
                     /locus_tag="ACG81A_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFS5014623.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2506..2754)
                     /locus_tag="ACG81A_001251"
     CDS             complement(2506..2754)
                     /locus_tag="ACG81A_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014624.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2753..2881
                     /locus_tag="ACG81A_001252"
     CDS             2753..2881
                     /locus_tag="ACG81A_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFS5014625.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2938..4632
                     /locus_tag="ACG81A_001253"
     CDS             2938..4632
                     /locus_tag="ACG81A_001253"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFS5014626.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4699..4905
                     /locus_tag="ACG81A_001254"
     CDS             4699..4905
                     /locus_tag="ACG81A_001254"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014627.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5283..6416
                     /locus_tag="ACG81A_001255"
     CDS             5283..6416
                     /locus_tag="ACG81A_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFS5014628.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6447..6767
                     /locus_tag="ACG81A_001256"
     CDS             6447..6767
                     /locus_tag="ACG81A_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFS5014629.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6796..7260)
                     /locus_tag="ACG81A_001257"
     CDS             complement(6796..7260)
                     /locus_tag="ACG81A_001257"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5014630.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7392..8645)
                     /locus_tag="ACG81A_001258"
     CDS             complement(7392..8645)
                     /locus_tag="ACG81A_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5014631.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8674..8991)
                     /locus_tag="ACG81A_001259"
     CDS             complement(8674..8991)
                     /locus_tag="ACG81A_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS5014632.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(9017..9337)
                     /locus_tag="ACG81A_001260"
     CDS             complement(9017..9337)
                     /locus_tag="ACG81A_001260"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5014633.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9801..11216)
                     /locus_tag="ACG81A_001261"
     CDS             complement(9801..11216)
                     /locus_tag="ACG81A_001261"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5014634.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11233..12549)
                     /locus_tag="ACG81A_001262"
     CDS             complement(11233..12549)
                     /locus_tag="ACG81A_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5014635.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
BASE COUNT         4042 a         2555 c         2195 g         3850 t
ORIGIN      
        1 ttcaaaaaga aaacatgtca taatatttcc catattgtgc aaaaaaatac aaggcggagg
       61 ctctactatg attgaagaat atattgagaa aaatatttta agacagctgt ttttatgtgg
      121 gcaattctat gtaaataaag aagtcaatct agaaaagctc tccaatctcc tgcatgtttg
      181 caaaaccact ttactcaatg atatcaataa cataaaaaaa gaatttgaag aacagatcgc
      241 ttatacacat cgggaaaagg accgttacac tttatatttt tcagaacata tacctcgatg
      301 caagatcatg cagcaattgt ctcaaaattc gttgttttta aaaacttgtc tgctttattt
      361 ggaagaagat gaacctgatt atcttcaatt aactgaatgt gaattcatct ctgtcagcaa
      421 agcttattca ttaaaaaaac aagtattagc ttactttaat gactgcggga tcgaaatcga
      481 ccgctactcc cctcgattca ctgaaatgga aagacgtctg ctgcttctta atgtgtcgta
      541 tcgtttaggc ggttttaatt cttgggagct acctgaatcg ttttttgaac gtgccgatcg
      601 tttcattgaa tccgtaacgg aaaatagtgg cagattttat gataaagaaa ataaagaaat
      661 cttatcgatt ggttttgcta tcagtttcct acgccagcaa gtgtgtgctg tgacaataga
      721 ttcaaaattt atcgaagaaa tcaaaaaacg tcctatttac aattatgtcg aatcagcatg
      781 ggaaaacact gatttccaaa cctattataa aaaagaagaa ttcgccttta ttttgacact
      841 attcaatctt tgtaattatg gtttccattc ttatcagttg attgcggaag actttcaaca
      901 gctacaccaa gtgtttattg acaatactcc cgaaataaaa gaactagttg ctacctttga
      961 atctcacttc aatcaagaat tattcggaaa ccaaccgttc gaacgtgcgc tgattcatct
     1021 gatgcgttcg gcttgggata actatcaatt attcatgcca gaaaaatttt atcttttaaa
     1081 tgaggaacag acaaatttat ataaagaagt ccagacaatt ttttccagct ggtccagcca
     1141 gttaccttat gatctacggt tgaatcctaa ttgcatgcgg gcttttgtga tcgaattgag
     1201 cggaattctc cgtctgacaa aagaacatct gactatctat atcgtcacaa attcggatgt
     1261 tcattatctg atttatcggg aagccttgga agctgtaact actttcgatt ttcaagttgc
     1321 accaacaatt tattcaagta tatctgatat aaagaagtat gcgcagcagt cctcaaaccg
     1381 ggtattatgt gaacgtacat tatatacacc agatgcggtc caatatgaaa atattattcc
     1441 tatttcgatc aatacgatcg atcgagctat catttcagct gtgcaaaaca aataaggggc
     1501 tgtgacaaaa atcatgtcac agcctcttat ttgttgaaga tgcccatttc tgtaacaatc
     1561 tcttgataca cgtcaggctc acaacctcac ttattttgga catctagggg tccgatctct
     1621 ttatttaaaa aataagaaat gatggttcgg ataactacca acgccgctag ttggaaaata
     1681 tcctgcaagg taggtttgat aatcgattcg atgatcccag ctgaaacaag aatttccaaa
     1741 cttagaagta tatagcctcc caacatcttt ttaatagcat tgttctcgct tactcggtcg
     1801 ctttgcactt ttgtatggac aaataaatat ttcaaaaaat ccttgattga tagacaaaca
     1861 ccccaaacca ataaacagat agcaaagata tcaagtccca gtacaagttt ttcgaaatat
     1921 ggaagaagaa actgcatcaa ctctgtcgtc atcctacctc tcctcttctt tgtttttctt
     1981 tgtcttctat ttcctgattc aagaaataag aaatgactgt tcgaatgata ataataagtg
     2041 caagcttcaa aatatctgtc caagtcggct tgataactga ctcgatgata tcagcaacga
     2101 ctagtacttc caagcttaac aaaatataac ttgctagcag ttttcttgta tctgcattac
     2161 gagtattacg ttctgcatag tttttcttga catgagacca atcaaaaagt ttgaatagaa
     2221 caagaacgat cccgacaatt aagataccga tagaacaaat atttaaaata gcaatgatcc
     2281 attctagaaa ttcaattaaa tttgagcgaa acatatacgc tacctacttt cttttaattt
     2341 gttcctattc ttttattgta aaagaaagtt accttgttcg tctatttaaa agttgttcta
     2401 atgataacgg ttcgcttaaa tttttctttt tcactctccc atttttctcc ctccatccat
     2461 aaatcgtgga agtatccaaa acaacgtagc aagttaaaat aaggcttagt ccgtcatcag
     2521 cttttcaaag aagcactgtc tcggtgcttt ttttgtaccc aaatttctaa aaatagaacc
     2581 atacacctcc tcaaatcttt tcttcttacc taccctattt ttatttacag gaaaatcaga
     2641 cacttcttct tttttgtcaa aatttccttt ttcttctcgt cctatcattt ggcattctaa
     2701 taaaaacgaa ctatccttgg atatagaaag agaaaacaag gaggcgtttt tcatgagtaa
     2761 aaaattaatc aaagtcggca tcggtttagg tttgcttgcc cttggcgctg catatctagg
     2821 aaaaaagaca ggactattcg aagatgacag tcatttatat gatgaattcg aatcaatcta
     2881 gcatcctatt tagttgaatc actacggaca tacttggaag acattggagg aatcacaatg
     2941 agaaaaagta aagcaataat gattggtgcc ggtattgcca atatggcagc ggctgtttat
     3001 ttgattcaag aaggcaaatg gaaaggggat cagatcacct tttattcatt ggatgatcac
     3061 ggttcaaatg atggtgcacc gaccgacact gtagtggatg aatattggaa taaaaaccat
     3121 ccattagaaa accaaaaagg ctatattgct cgaggcggtc gcatgcttaa ttaccgtacc
     3181 tatgtagatt tgatggatct gttaagtcga ataccttctg ttactgagcc agggatgact
     3241 gctgaagaag atacgagaga ttttgatgca aaacaccgaa cctttgacaa agcacgtttg
     3301 atggaaggtg gcaaaggaat cattgatggc ggaaaactgg gattcaataa taaagaccgt
     3361 attcttctga ctaaactgat tgctatgcct gattcagaag aagaaaaact agacaatatc
     3421 actatcgaag aatatttcaa agatgatcca cacttttttg aaacaaactt ctggtttatg
     3481 tgggaaacaa catttgcttt cagaacacgt agttctgctc aagaattgcg tcgctatatg
     3541 catcaaatga tttatgaatt cactcaaatc gaacatctag tgggcgtcaa tcgaacacgt
     3601 tacaatcaat acgaaagtat tatgcttcct ttgatcgagt acttgaaaga acaaggatgc
     3661 aaaatcattt tgaaccgccg tgtcactgac tgggaattca aagaaacacc gatgcaagat
     3721 gaaatcactg tgactggttt gaaaattacc aatacactga ctcaagaaga agaatacgtt
     3781 gtggtcgatg aagatacagc agttattttc actaatgggt ccattacaga ttcagctact
     3841 ttaggagatt acgaaacacc agctgtagaa aatatggatt atggtgcagc ttctagtctc
     3901 tggaaaaaag ccagtgaacg tttctacaac ttgggaaatc ctgacaaatt ctttgctgat
     3961 cgagatgcaa gcgaatgggt cagctttacc ctgacgacga aaaaccattt gttgttgaat
     4021 gaaatcaccc gtatcacgac tcaagaacca ggaaatgcct tgaattcatt tatttcaacc
     4081 acaccgatca cgccgttagg ccaaaaagat gtcaatatgt ctattgtcgt gcatcatcaa
     4141 ccgcatttta cgtctcagaa accaaatgaa acagttattt ggggttattt cctttatcca
     4201 agacgacgtg gcgaatttgt ggataaacag tatattaaaa tgaatggaaa agaaatgcta
     4261 gaagaactga ttggacaact ttcaaaagta gatcctggac cagtgaatat tcgtgaaaaa
     4321 gaaacagaaa ttttcgacag cgtaataaat aatatccctg tctatatgcc ttatgcttcc
     4381 gcgttgttca ataatcgtgc caaaagcgat cgaccaaaag tcattcctaa acattcgacc
     4441 aatctggcct ttacaggaga atttgtcgag cagccttatc agatgatctt tactgaacaa
     4501 agtgctgtac gttctggaga aatcgctgct ttccattttg cgggtattcc tatgtcccgt
     4561 cttgtcaaaa caccgcgtta cgacaaagat attcctactc tagctcgtgc cgcaaaaaaa
     4621 atgtttgaat aatcaagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacaacc
     4681 aggctaaaac agcttcttat gtttttgatt tggttggact tattaccgca gatgcaagct
     4741 tttgaacacc gtttaccaag aggttatgaa aaagctgccc tgcatcaagt aataagagca
     4801 gccaagctaa aacagcttct tatgtttttg acttggttag acttattacc gcagatgcaa
     4861 gcttttgaac accgtttacc aagaggttat gaaaaaggcg tttagtttcg agtatgaaga
     4921 aaaattttgt gaaaatggtt cctcacgttt ttgcataaat ttgacttaat accgagaaac
     4981 tgatcccttc tgtcataacc tctttttatt tatgtctgtt tctttttgaa aacaactatt
     5041 ttttcaacgt ttactctctt gccaatccaa catcatctaa cggatacaac tatttcaatg
     5101 attaccatta cactaaaaaa tcggaaacac acctctccga acacaaatac ataagactat
     5161 agaatgtttc atttaagaaa aaaagcgttt cattatcaaa aaggtaagat ctcctttaaa
     5221 atgaaattat ctgtagtact aataatgcag acaattttat gaatctgaaa ggagcatctc
     5281 caatgaaagc agtcacttgg caaggaaaac agaaaatgga aatcagaaac gtagatgatc
     5341 caaaaatatt agaaccaaca gatgcaatca tccgtatcac agcaacagcc atttgcggct
     5401 ccgatctgca tctttaccat catggtgaag cagttttgga aaaagggtat gttgtaggac
     5461 acgaaccaat gggaatcgtt gaagaagtgg gatctcaagt tcaaacattg aaaaaagggc
     5521 aacgtgtggt tatccctttt aatattagtt gtggtcattg tcatttctgc ctgaatcaca
     5581 tggaaagcca atgtgatgaa tcaaatccag atcagttata tggcgggctt ttcggatttg
     5641 gcaagttgaa cggtaatcat tggggtggtc aagcggaata tctaagagtt ccttatgcag
     5701 actctacttc atttgttgta ccagataatg atcttcctga tgaaaaagtt ttatttctat
     5761 cagatatttt gcccacagct tattggagcg ttgaaaacgc aggagtaaaa aaaggcgata
     5821 cagttgtgat tctaggttct ggtccagtcg gtctattcgc tcaaaaattc gctgttatgg
     5881 ctggagcaga gcgcgtaatc gcagtagatc cagtacagca ccgtctagat aaagcggcta
     5941 attacaatgg ggttgaaact tatttcttag aagacacggc acaagctgga acagaattgt
     6001 atgaactggc taaaggtggc gcagatgtca ttatcgattg tgtaggaatg gacggattag
     6061 agcctgtcaa agaaaaagcg aaaaatttag taagcttaca aagcggcaca atctctccat
     6121 tacagatggc atcgcaagct gtcaaaaaat ttggaacagt ccaagtaact ggtgtttata
     6181 tgactccagc ttcttcttac ccgctgcaag aattttttat gcgggatatc gctgtcaagc
     6241 atggacaggc accaattatc catctaatgc caaaaattta cgcaatgatt gcagaaggac
     6301 tattcgatcc tacacaaatc attactcatt cgctgccact atcagaagca gcacaagcat
     6361 atgagatctt tgacaaaaaa gaagataaaa atataaaagt tgttttgaaa ccttgaattc
     6421 agtaaaaaat acaggtgatt ttctttatgc ccaaaatcaa acgaaatacc catgagatcg
     6481 aaataggaaa agaagaagat atcgaaatac gaggacgtcg cttccgcttc tatttccaaa
     6541 atcgttacac attgatctca ctggctgtcg atttattaac cgggattttc tatatttttg
     6601 gaagccttgc ttccttaact aaaatccccg atcgatatgg cacctatttt tatttggctg
     6661 gtgctatttt cttgacgatt cgtcctattc tcagaatcgt aaggaacatt tttatttacg
     6721 acgaaaaaaa agcaatgaag agaacaagca aaaaaaaaga ggcatgagaa tttttcaaat
     6781 ctcatgcctc ttttttcacc ctatcgtatc actttgatac agacgagcgg tcgtcccact
     6841 ttcttccttg accattgtca aaaatgtcca ctcgtatcgt tcataaaacg tggtatgatc
     6901 tgtaattaag taaagttttt cgtacccaag ttgatgcatt tcttttctga tttccgctaa
     6961 tatccgcttg gctatcccct gcttacgctt ttccggttca acaaacaggg cacaaagatt
     7021 tggtgtcaga tcagggcggt tatggaagtc gttttcaatg acgcccgctc ctccgacgat
     7081 ggtttctttt tcatctaaga ccacatacca ttgaggaata ctttccttat tagcgattcc
     7141 agtacgaata ctttcctcat acacaacagc aggaactccc cattttgatg aaaaccaatt
     7201 cgcgctttgt tttactaatt ctgggtgttc tcttaatttg atccagttat atgtaaccaa
     7261 tgtttaaacc tccgactaaa ataaaacgtt tacaaaaatt atacaagtct ttatgaaaat
     7321 tagcaatgac aacaatagtg atgtaaaaaa aaaaacggaa aacaatcact tgttctccgt
     7381 tttttgatta atcaagcatc taaagcttct tttcttcttt cttggatcgt agctaccttg
     7441 acgaacggta gatagatcaa aatagaaaga accaaattga cagcggctag tacaccacct
     7501 gcaatacttt ttgttgccaa tacaccgcca ataattggtg gagttaccca tggaggcata
     7561 acggtagctg ctggcactaa acctgtagaa gtcgcaaagt aagcaacact aactaagacc
     7621 attggtgtca aaataaatgg aatgaacatg atcgggttca acacgatagg taacccaaac
     7681 atcgttggtt cattaatatt gaagacacct ggcgcaatcg acaagtttgt tacaaccata
     7741 tatggtttgt ttttacgtcc tactaaataa atagctaaca gtaaacctaa agttgcacct
     7801 gttcctccta agttgacaaa gctgtcaaag aaaggtttgt tcacgataaa cggtgcgcta
     7861 tgtccagctg cgattgcatt aacgtttgct tcaatggctg gcgcattgat tgtttgcatc
     7921 aaaggatcga ccatattcgc tccgtgtaaa ccaaagaacc ataagaatgg tgtgataaat
     7981 gcaagcagta aggcagatgg ataagagttt gcaagtccca taaatggttc ttggaccact
     8041 gtatagaaag aacctacgat atcatcaaca ccgaatgcag caaaaattgc agcaactaaa
     8101 gcaaacgcag agatagtgat cattgctggt agtaatgaag caaatgcttt tgcaactgcc
     8161 ggtggcacac catcaggcat tttgatgatc aatttttcat ttccgcttaa acgtacaaag
     8221 atttctgttg aaataatccc aacaataata gcaataaaca aaccgtttga gctcatacct
     8281 aatgctccgc ctaaagcgaa gaaagagcct aaagaaatca cacctgcgga aacaccgtct
     8341 tttcggtagc cgttagctaa atgataagca agcaagaaag caatcaagac agaaagaatg
     8401 gcaaatgtac cgttccaagc agcagccccg aatcctttcc atgattcacc accaaaaatt
     8461 gagttcatca attcttggaa cccgggaatc ggtaagttat tgatcattac tgctaatgct
     8521 cctaagatca ttaacggcat actcaccata aatgagtccc gaatcgcaac taaatgccgt
     8581 tgtgcaccaa tttttgatgc tacaggtata aaatgtttct ccataaatgc aacaaattta
     8641 tccataaatc ttccctcttt tctttactgt agattacgcg ttcaatcgac gatacagatt
     8701 aatgatctct tttgctaaat ctgtaaaagc tatagatgtc atcagatgat cttgactgtg
     8761 gaccgtcaaa agcgtgactt caacgtgatt tccttgtgct tcttgtgtca gcatctctgt
     8821 ttgagagtga tgtgcttgga ctaaagattc ctctgcatct ttgatttttt catctgctaa
     8881 agtgaaatca ccttttttcg cagcagcaat cgcttccatt gcatcgcttt ttgcatttcc
     8941 gccaaacatg atcagaccca tgatggcttc tagatttttt tgatcttcca tttattgtat
     9001 cctccgataa tttttattat ttgtttatta gtttttctgc ttggtcgagt actttttcac
     9061 cattcatcat gccatagtca gccatattga ttacatctaa aggaatgcct ttaggtgcta
     9121 gcttttcagc aaattgattt tgcataaagc gtacttgcgg tcctaaaagt aaaacgtcta
     9181 catttttaga ttctagttga ttatccgcat ctgaagcaga tacagcaaag atgtctgctt
     9241 ccattccttt tgcttctgct gccttttgca tttttgtgac taggagactt gtactcattc
     9301 cagctgaaca tacaagcatg attgttttct tttccatttt aaacacgttc ctttctatac
     9361 ttacacttgt atataagcaa gtagcgtgcc aactttaaaa gcgctttaaa aagttgatat
     9421 catcacattt aaggtctata ccaagttgca tacacactaa aaaagaaagc gcacactaaa
     9481 cacacaaatt aatttttatt accttttttc ttttaacgtg cacaaaaaaa gttgtgacac
     9541 aaaacttgtc acaacttttt tcaaataaac ggtattcaaa agcttgcatc tgcggtaata
     9601 agtccaacca agttaaaaac ataagatgtt gtttttactt tgctattctt attacttgat
     9661 gcaggacagc tttttcacaa cctctcaata aacggtgttc aatcagctga ccctgggtat
     9721 tagctcaaaa cgctggattc acaacctctt ttatttcaaa taaccagtaa atacaatgaa
     9781 catctacaaa aaacttctat ttatttatag atttcgtcag gcaaatccaa ttctctctta
     9841 tcaatcaaag tctgatacca cgaagcagat tttttcaatg ttctgcttcg ttcatcgttc
     9901 aattcgattc gaactaatcc atatcgattc ttaaatgcat tcattggtga cacacagtca
     9961 gtgaatgccc ataacatata gccttcgcaa tttgcgcctt cttctgttgc tttgatcaag
    10021 taatgcaaat gctgtccgat aaattcgata cggtaatcat cttgcacgat tcctttctca
    10081 tccatatact gttcttcata ttctcttccc ataccgtttt ctgttatcag ccaagggata
    10141 ttatgataat tttcttttag atacatggct atatcataca tgattttcgg atagatttcc
    10201 catcccctgg aattattcat tttcttccca ggtaaatcaa agacttcata atatcgttca
    10261 ggatggaaag gtgtctcttc attccattga taacgagggg cgcgaacgcg tttaggataa
    10321 tattgattga ttccaaggaa atctactgta tgatgctgaa tcgtttctaa atcctctgtt
    10381 gcaggatcga aataaatcga ttgtttttga cacaactcca ggaactcttc tgtgtagtat
    10441 ccttttacca tcggatcaaa aaatactcga ttataaaaca aatcatacat ataagcagct
    10501 ttttggtctt ctggagaatt ggatcgagca tagaccattt caggatttaa aatacagcca
    10561 atccttccac cataatttcc gctgcggtaa atctctactg ctttagccgt tgctaaaact
    10621 ttatgataat tccacatcat ccactttttt gtgttttgtt catgcggcca gcgaacagca
    10681 tccaaatagc aacgcgtttg gggaacgatt ggttcgttga atgtaaacca gttttttacc
    10741 ctatcaccgt aacgatcaaa ggcaatttga gcatacttag cataaaaatc aactactttt
    10801 cttgaagacc agccatcata tttttccaaa agaaaagctg ggacttcata atgttccaaa
    10861 caaatcatcg gttctacgtc tgcttcgatc aaagagtcga tcatttcact tatatgctga
    10921 gcatattctt catctactat acagttctca taatcagtaa aaaaacgagc ccaattgata
    10981 gaagtccgat aatgtgtcag atggatggat ttcatcagat ccacatcctg ctggtattgt
    11041 tccataaaat tagttgctac ggctgggcca tacccattat gccaaacaaa atgttcattt
    11101 ttataccagc tatctataaa cgaatcctgt ccttcttttt ttcctttcca accttccgtt
    11161 tgccaagcag aagatgcagc accaagaata aaattttttg gaatagtaat tttcatgatt
    11221 tcctcctgtt ttttagatcc catcattttc cacacgattt gctactttta caaatggcag
    11281 atagatcaaa atagaaacaa taatacaaac aagttgcgta ataactgcac cgatatttcc
    11341 tgctgttgcc agataagcat tgacgatcgg cggcattggc caagggacca ttacaactgc
    11401 tttcgccgca aatcctaaag cggttgcaaa ataaccaatc gttccagtga taagaggcgt
    11461 taagataaac ggaatagcta aaattggatt cagcatgatc ggcataccaa aaataactgg
    11521 ttcattgatg ttgaagatcc caggagcaag ggataacttg gtgatttgtt tcatatcttc
    11581 ccgttttcca actaacaata ctgcaatcag taaaccgatc gtgactccag acccgcccat
    11641 actcatatac atatcccaaa atggcatagt aataatgttc ggtacttctt ttccagcttc
    11701 aaaagcttcc gtatttacag caattgccgc taaaagcaac ggttcccgta tcggcttcac
    11761 catttgattt ccatggatac caatcaccca aaagacttgc gcaataaaca tcaatagcaa
    11821 aatacccggt aacccttgta cgacaccttc taatgggcgt tgtacgacat tgtaaatgat
    11881 atcataaagg tacattcctg taagttcttt gatgataaat cccgcagccg cgatacccga
    11941 tacagtcaga atcgtcggga ataaagcaga aaaactagca gaaacattcg ttggcacggt
    12001 atcaggcatt ctcagctgta gtttcttctg tttcctcaac cagcaataaa gttcaataga
    12061 aaggatggag accaacattc ccaaaaataa ccctttcgta tctgtatatt gtttagccaa
    12121 tacgttttct actataacag tctgattttc attcactaat tcataaacag ttggattaac
    12181 agataaataa gacatcactg cgatgatacc agggaataca ccatccactt tattcagctt
    12241 ggctaattcg atccctatca gaaaaacagc atagatggtc aagaaactta gtgtgacata
    12301 gctgatggca gatgcgatag gctttaattc ttctaaaaaa cgcaagaaac ttatctgtgc
    12361 taagccattt tccgagtcaa agaccattgc tgaaaacaat gtgccaaaag cacccgtaat
    12421 aattaccgga atcaatgcag caaatgcgtt ttttatcacc atgatatacc ttaaattatt
    12481 gattctcgtt gccacgttcc ctaaaacatt ggctaattta tccataaaac tcattccaga
    12541 tcccctcatt cctgcttctt ctccttgacg tcatctcttt ttgtctatta gtcagcgctt
    12601 acatttcgat gaacagaaac tatttactcc tcttttttac ca
//
LOCUS       DAZULY010000071        12600 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000071 DAZULY010000000
VERSION     DAZULY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.072"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..365
                     /locus_tag="ACG81A_001263"
     CDS             <1..365
                     /locus_tag="ACG81A_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase"
                     /protein_id="HFS5014636.1"
                     /translation="LIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFY
                     TKADTKTYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITA
                     WNTFVELKKKRRRTQKFL"
     gene            493..1176
                     /locus_tag="ACG81A_001264"
     CDS             493..1176
                     /locus_tag="ACG81A_001264"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFS5014637.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1485..1558
                     /locus_tag="ACG81A_001265"
     tRNA            1485..1558
                     /locus_tag="ACG81A_001265"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1519..1521,aa:Arg,seq:tct)
     gene            1717..2076
                     /locus_tag="ACG81A_001266"
     CDS             1717..2076
                     /locus_tag="ACG81A_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFS5014638.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2199..3830
                     /locus_tag="ACG81A_001267"
     CDS             2199..3830
                     /locus_tag="ACG81A_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFS5014639.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(3892..4533)
                     /locus_tag="ACG81A_001268"
     CDS             complement(3892..4533)
                     /locus_tag="ACG81A_001268"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS5014640.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4719..4795
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4815..5123
                     /gene="rplU"
                     /locus_tag="ACG81A_001269"
     CDS             4815..5123
                     /gene="rplU"
                     /locus_tag="ACG81A_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFS5014641.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5150..5494
                     /locus_tag="ACG81A_001270"
     CDS             5150..5494
                     /locus_tag="ACG81A_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFS5014642.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5507..5800
                     /gene="rpmA"
                     /locus_tag="ACG81A_001271"
     CDS             5507..5800
                     /gene="rpmA"
                     /locus_tag="ACG81A_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFS5014643.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6234..9041
                     /locus_tag="ACG81A_001272"
     CDS             6234..9041
                     /locus_tag="ACG81A_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5014644.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9232..9723
                     /locus_tag="ACG81A_001273"
     CDS             9232..9723
                     /locus_tag="ACG81A_001273"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS5014645.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9826..10815
                     /locus_tag="ACG81A_001274"
     CDS             9826..10815
                     /locus_tag="ACG81A_001274"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFS5014646.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            10848..11651
                     /locus_tag="ACG81A_001275"
     CDS             10848..11651
                     /locus_tag="ACG81A_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFS5014647.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11672..12586
                     /locus_tag="ACG81A_001276"
     CDS             11672..12586
                     /locus_tag="ACG81A_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5014648.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
BASE COUNT         4002 a         2121 c         2745 g         3732 t
ORIGIN      
        1 atctgatata tgtacgaccg aaaaatacaa atgagatttt agtcggagat attattacat
       61 attatgaaaa ttcaggaaaa aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc
      121 acgaagattt ttatacaaaa gctgatacaa agacttatat tgagcctgga gtagtaaaaa
      181 agagaaatat tatcggtagt cccatctttc agattccata tattggacta gtcctttcaa
      241 aaacggcatt tgcatggatt caggggttat tctttacgat tgctttttgc ctaacaggaa
      301 tcacagcatg gaataccttt gtagagttga aaaagaagcg gagacgaaca cagaaatttt
      361 tgtaagaatg ttaaaaacat aaatgtattc cagagatcca acgactcttc tgaacatgaa
      421 acacgagaaa acactcatgc agcttgtaga tttagtcagt tgttcgtata atgtgtgaag
      481 aggaggagta gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga agaactattg
      541 gattttatgc gtacccagca aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa
      601 gctcatgtag cagaagtacc agttattccc catgaaacag ttgtattttt gaaattttta
      661 cttggacagc ttcagcctga acgaatcttg gagatcggag cagctatcgg tttttcatca
      721 agtgtcatgg ccacgacaat cggagaaaac gctcatgtta cgacaattga tcgtttcgat
      781 gtcatgatcg agaaagctaa aaaaaactat gaacgtctag gactgacaga taaagtgact
      841 ttgcttgaag gtcaagcagc agatatcttg ccggagttgt ctggtccgta tgactttatt
      901 tttatggata gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt acgtttgtta
      961 agaaaaggtg gcgttttgat ggtagatgac atcttccaag gaggaacgat cttgctccca
     1021 gatgaagaga ttccccgtgg caaacgagct atccatcgta aattaaatga atttcttcgt
     1081 gttgtgatgg atcatcccga tttgacatcg acaatcttgc cattaggtga tggtgtgatc
     1141 ttaatgacaa aggaatcaga aacaatcgag ttgtgataca tgattatggc agcctgtaga
     1201 taatcgattg aatcgagggc tcaattcgaa cttgatgtaa aagtttctat atttgtaagt
     1261 caattaaagg atacaggaaa tctaaataaa caggagtagc atgaagtcaa tgtagctcct
     1321 gttttcaatt gataacctta gaacagctga aggagaatgt ctcgttttaa aagattgatt
     1381 tagtagttgg gccaaaataa ttcattattt gctaattaaa taactatttc gtattgacag
     1441 aagcaattta attatctata attattgatg ttccgctgat tcttgtcata gtagctcagc
     1501 tggatagagc atccgccttc taagcggacg gtcgggggtt cgaatccctc ctgtgacgct
     1561 aaaaaaccgt tagaaactga ggtttctaac ggtttttctt tgttttttgt gccaatattg
     1621 tttgttttca aaggcaaata aaataaaaaa acatccttat cgagtacaat tataaggagc
     1681 aaaaaaataa aactcatcag aaaagaggaa gaagatatga cgatactatc tagaatttta
     1741 gtgactctag ttgcaattga atttttttat atcatgtata tcgaaacggt gagaactgat
     1801 tctgacacga caagtagggt ttttaaaatg agtaaagaag agttaagcag aaaatctgtt
     1861 caaacattgt ttaagaacca aggagtgtac aatggactat taggagtggg actactttat
     1921 ggagcttatc tttcttccgc atctaaagaa atcacaagta tgctattaat atctattttt
     1981 tttgttgcat tatatggcag tttgactagc gatcgttgga ttttactcaa acaaggtggg
     2041 ctagctctgt tggcattgct ttctattttc ttataaatat agaaaaaata agaatttatc
     2101 taacaggaaa ctttgtgaaa aatattactt tatctcatga aatatgatat tctaatgaat
     2161 ggtattttag aaaatttcat gaaaaaggag ttagataggt gttcaaaaat tatgtttcct
     2221 cactaaaaaa agaattttca ggttatgatc tttcaagatt tcagaaagat cttttggctg
     2281 ggatcacagt agctgctgta gcattgcctt tagccttggc ttttggggta tcaagcggag
     2341 ctgatgcggc cgcaggtttg attacggcta tcattgccgg tatcgttatt ggcggtttat
     2401 caggcggttt ttatcaaata tcaggtccga caggtgcgat ggcagctatc ttgatgtcta
     2461 ttgcggcaac aaagggaatg caaggtgtat tgcttgctac ttttttagcg ggtctatttt
     2521 tacttgcagc aggacttttc cgattaggaa cactgacttc ttttatccca gcacccgtaa
     2581 ttacggggtt tacttcaggt attgctatca ttattgcatt aggtcagatc gataatttat
     2641 ttggtgttca ctcggaaggt acaaacgtta ttgaaaaact aagtagttat caaacgatag
     2701 gatttccgat aaacacagcc tctttgatca tgggagggct agtcatttta gggatgttta
     2761 tctatccaaa aaaatgggcg aaacgaatgc ctagttcgct gctggctatc attttggcaa
     2821 cagcagttat gctattgttc catttaccag tagcaactgt gggtaagatt ccacaaacat
     2881 tgatcagtag taatcgtttg aatatgggag attttagctt ttctgccttg caacaagtgg
     2941 cagttccagc tattagcatt gctttattag gaatgattga aagcttgcta tgtggtgctt
     3001 cggctggccg aatggcaaat aagcccttag atagcaatca agaattaatg gcgcaaggaa
     3061 ttggaaatct gttgcttcct ttctttggcg ggattcctgc tactgcggcc atcgcccgta
     3121 caagcgtggc catcaaatct ggtgcacaaa ctagattagc tggtatgata catgcagttg
     3181 tcttactgct ctccatgctt gtttttgcgc cagtgatgtc acagattcca atgccagcac
     3241 ttgcaggtgt tttgattgtg acggcttggc gtatgaatga atgggaaacg atcaaggagc
     3301 tatttactaa taaatattgg tcagcagttc tattgtttat ccttacgatg atttgtacag
     3361 ttatctttga tttaagtatt gctatcgtga tcggggtgat cggcggctgt gtattttttg
     3421 ttgtcaaaag cgctgctatc acgatttcat tagaaacgat cgattggcaa agaatggggc
     3481 tgccagaaac ggaaaagctg aataactgga cagtagtgta tattagtggt ccactgtttt
     3541 ttatgtcttc agaaaaactc aaaaataaat tggaagaact aaatcatcac gatggtgtca
     3601 ttttctccat gcgaggcgta ccaagtattg atttgacggc acgtgcagtt tttgatgaat
     3661 ttcaagaaaa agcagagcaa agaggacaaa cgattatcta tacttccttg cagccagaag
     3721 tagaaaaaca actttctcac ttgtgggaac atacattcac gcatcaacat caaacagttg
     3781 ctcatgcgtt gacagcttta catgcgaaat atactgcaat tagccattaa aacaatgaaa
     3841 aagaacagac aagactgtaa actgaagatt ttatggtctt gtctgttctt tttaatattg
     3901 ctgtaaaagt gaagctagta cttgccagtc ttgatggata acaggtacaa gggagcgatt
     3961 gtagaaaata gctgctggat gaaaaagtgg aagaattcga tagttttttt ctgaccattc
     4021 gtaggcatcc ttttgttctg ttaattgtaa gacaggccct gtatagagtt gaccatgatt
     4081 tttggtaacc gtatattctt tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc
     4141 aattaatttt ggctggacct tcatcagttc gtaatccaat attggtgcat gcgcaaatac
     4201 ttcggaacga gtaggtgtcc gatttggata aacagtttct gactgttgtg tccgtttatt
     4261 gatccgatga gtcacgcgat acgggcggct ccttacagca ctggtgatat acacctcttc
     4321 tcgtgttaag ccagttgaag aaagtgcctg catcaattct tttccagctt gaccgctaaa
     4381 aggaacatgc gtatcaatct ctgttttccc tggggcttct cccacgagca tcagtttagg
     4441 atgaagaggt ccttgcccag cgataaatcc ttctacagga cggccgttca ttcttaaaga
     4501 agcttcttgt atcagttctt ttgggtattc catttatgct cctccttttt cttttattat
     4561 aagaataaaa gaaacacaat gcgactattt tatttggtag aactgtgagg atagaaaagc
     4621 agaagtattg cttgaatttt tgttttactt tttgatttta aatttagttg actaacggtt
     4681 gctgttctgg taagctagta ctgttgtaat tgtggactcc acagctacaa ccgctcagaa
     4741 caagtaataa gcacagcata tgctgtcact tcggatggcg agtctaagta tatcagaagg
     4801 aggtgcttta tagcatgtac gcaattatta aaactggtgg taaacaagta aaagttgaag
     4861 tgggacaagc aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg
     4921 acgaagttat cttagtaggc ggagaatcta caaaagtagg cgctccaact gttgcaggtg
     4981 caactgttga aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat
     5041 acaaacctaa aaaacattca caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg
     5101 ttatcgacgc gatcaacgct taatagcgtt tgagaaagaa ggcctatata tgatcaaagg
     5161 aacatttaag cgaaatgacg caggtcagat cgtttcattc actttgaccg ggcatgctga
     5221 tgctggtcct tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac
     5281 tgtcaacggg attgcctcat tagccggttt tgagccgatt gttgaaatga atgaagaaga
     5341 aggcggttat ctttataccg aagtcacttc aggaatgaca caagaacaaa acaatattgc
     5401 tgagattcta ttagaaaatc ttttattagg attacaatcg atagaagctg aaaactcaga
     5461 atatatccaa attaaaacaa ttaatgataa ataggaggtg cagaccatgt tgttatctat
     5521 gaatctacaa ttatttgccc acaaaaaagg tggcggttct acatctaacg gccgtgactc
     5581 agaatcaaaa cgtctaggtg ctaaacgtgc tgatggacaa acagttacag gtggatcaat
     5641 tctttaccgt caacgcggta caaaaatcta tccaggtgta aacgtgggta tcggtggaga
     5701 cgacacttta tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa
     5761 aaaacaagtg tctgtttatc cagtagctca agaagcttaa ttgaatctta tgattaaaga
     5821 ggttgtgaca agacttttgt cacagccttt tttgtttata aaaataaaag ttgtagtgga
     5881 ggctacgaag tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg
     5941 atgttttgat aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat
     6001 aattatatgg taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt
     6061 ttcgttcttt taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag
     6121 gtaaatagtt gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa
     6181 tagtctatag aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg
     6241 tcaaagaaaa aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg
     6301 aaattgctga agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag
     6361 tgcgcgaagg aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac
     6421 tcgttacgca cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct
     6481 ctgaagaaaa aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag
     6541 ctaacggcag catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa
     6601 gaggattaaa ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg
     6661 cgatgtatca ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt
     6721 tcaattgtgc tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg
     6781 caaaaggtgc ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg
     6841 gcggtatgct gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct
     6901 tttactttat ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag
     6961 caaatgtacg gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt
     7021 ttgttcggcg tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg
     7081 agaaagtcga tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga
     7141 tttctttgac agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag
     7201 gacaattgaa gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat
     7261 cacccgaaat ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc
     7321 aattagcaag caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa
     7381 ctgtagcacc aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata
     7441 atctatatga tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag
     7501 aagcaatcaa tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag
     7561 accatggttt ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca
     7621 tagaagtgac caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc
     7681 aaaactttat ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag
     7741 gagaagaacg gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat
     7801 atgctgttgc gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag
     7861 aaagtgaaga ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg
     7921 gcagaatcgg tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag
     7981 ttgtccaaca gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg
     8041 atccgaaaac agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg
     8101 gggtcatact cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag
     8161 acactggaat tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg
     8221 tacgaaaggt ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt
     8281 ttcgaggtta cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata
     8341 agaaaaaagc tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag
     8401 aattgatcga acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt
     8461 cgattgtgga agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta
     8521 ctactgggat tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg
     8581 atcaaaatat cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg
     8641 aattcatctc cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata
     8701 acttcatctt tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa
     8761 tctctcagac gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc
     8821 acacagcagg aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg
     8881 aattgacaaa tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt
     8941 tggaagatca gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag
     9001 tccataatca gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac
     9061 ttttatagtg tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc
     9121 gtaaaaaagt tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca
     9181 cagaaatgat aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt
     9241 cgattggcac gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag
     9301 caaacaggtg ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga
     9361 aagtttctac taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag
     9421 aaaatgattc aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc
     9481 accaatcccc aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat
     9541 atcggcggta tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat
     9601 caacaagata ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga
     9661 aaagtacctg ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg
     9721 tagcaagaat ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta
     9781 atatttttga atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc
     9841 ctagcaagtc atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc
     9901 gaacaagaaa acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga
     9961 gccaaattgg aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt
    10021 gatttatggg gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat
    10081 aaatgggcaa tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc
    10141 ttttcaatgg aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt
    10201 gtaaaagtaa aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac
    10261 gcacaaccta aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat
    10321 gtattagcac gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa
    10381 gcagtgcaac caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt
    10441 aagagactaa tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc
    10501 aaaatgatcg aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt
    10561 gaaaatcctg aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat
    10621 gttgggtcaa tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga
    10681 caagaagacg ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt
    10741 aaagtaccta atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa
    10801 ttagcaaaag cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt
    10861 caataatttt agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat
    10921 ttcaatttca tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg
    10981 aagctggtat cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg
    11041 gtgccgctgt agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt
    11101 taggtggaca aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg
    11161 ttgcagggtt gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga
    11221 tggatgcggc tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg
    11281 tatgtttaca aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac
    11341 aaactgtgca atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg
    11401 gtgggggaat ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag
    11461 aagtatggcc attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga
    11521 ttgctctagg tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg
    11581 gtggttcatc aaataatgga ggcggaagca actctggtga ccctttaggc gacattttga
    11641 atgactatta atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa
    11701 aaaagatcgt ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga
    11761 acgtatgcaa aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa
    11821 aacaaaagaa gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc
    11881 atacattgct tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg
    11941 cgcgcctgtt gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc
    12001 tggtgttggt gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc
    12061 ttcattagct atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat
    12121 tattcgttgg gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat
    12181 tactgatgac ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg
    12241 gatgttcgta ttagctgccc ttgtacaaag atgggtatca atcaaattct taccagttgt
    12301 atcaactgta aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg
    12361 tgaaggaatc aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa
    12421 ggtaacaaca ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt
    12481 aacattgtta tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg
    12541 tttattcgta gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata
//
LOCUS       DAZULY010000072        12530 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000072 DAZULY010000000
VERSION     DAZULY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.073"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(144..506)
                     /locus_tag="ACG81A_001277"
     CDS             complement(144..506)
                     /locus_tag="ACG81A_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014649.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(580..960)
                     /locus_tag="ACG81A_001278"
     CDS             complement(580..960)
                     /locus_tag="ACG81A_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFS5014650.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1042..2217
                     /locus_tag="ACG81A_001279"
     CDS             1042..2217
                     /locus_tag="ACG81A_001279"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFS5014651.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2294..2878)
                     /locus_tag="ACG81A_001280"
     CDS             complement(2294..2878)
                     /locus_tag="ACG81A_001280"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5014652.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3079..4077
                     /locus_tag="ACG81A_001281"
     CDS             3079..4077
                     /locus_tag="ACG81A_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5014653.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4189..4698
                     /locus_tag="ACG81A_001282"
     CDS             4189..4698
                     /locus_tag="ACG81A_001282"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFS5014654.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4728..5387)
                     /locus_tag="ACG81A_001283"
     CDS             complement(4728..5387)
                     /locus_tag="ACG81A_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFS5014655.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5389..6153)
                     /locus_tag="ACG81A_001284"
     CDS             complement(5389..6153)
                     /locus_tag="ACG81A_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFS5014656.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6161..6838)
                     /locus_tag="ACG81A_001285"
     CDS             complement(6161..6838)
                     /locus_tag="ACG81A_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFS5014657.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6875..8260)
                     /locus_tag="ACG81A_001286"
     CDS             complement(6875..8260)
                     /locus_tag="ACG81A_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFS5014658.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8470..8554)
                     /gene="ffs"
                     /locus_tag="ACG81A_001287"
     ncRNA           complement(8470..8554)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG81A_001287"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8577..9092)
                     /gene="tadA"
                     /locus_tag="ACG81A_001288"
     CDS             complement(8577..9092)
                     /gene="tadA"
                     /locus_tag="ACG81A_001288"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFS5014659.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9199..10056
                     /locus_tag="ACG81A_001289"
     CDS             9199..10056
                     /locus_tag="ACG81A_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5014660.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10137..10472)
                     /locus_tag="ACG81A_001290"
     CDS             complement(10137..10472)
                     /locus_tag="ACG81A_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014661.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10539..11909)
                     /locus_tag="ACG81A_001291"
     CDS             complement(10539..11909)
                     /locus_tag="ACG81A_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFS5014662.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(11987..12523)
                     /locus_tag="ACG81A_001292"
     CDS             complement(11987..12523)
                     /locus_tag="ACG81A_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5014663.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3721 a         2551 c         2103 g         4155 t
ORIGIN      
        1 tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc tttagcagaa
       61 catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa aattcaaact
      121 tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc gtaagttcca
      181 agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa ttcttctctt
      241 tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt aatatgtgta
      301 tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc ttttaagaaa
      361 ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc aaattccaac
      421 ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg caacataatt
      481 gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt ctatggctta
      541 cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat tttctttccg
      601 ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa tagtggtaaa
      661 tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa cagctggatt
      721 tgttttttct aacgttctta aagataaact gatttttttg gaatattcat caatgtcgat
      781 gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt tcgtataacc
      841 ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag aaacgaatgc
      901 gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt tataagtcat
      961 ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa agaagcgacg
     1021 aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag aaaagataca
     1081 gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt acttcctttg
     1141 tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt tagtgaatac
     1201 atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca ggctatcatt
     1261 cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt ttttacaagt
     1321 ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg cgattctatt
     1381 ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa tcaacgtcac
     1441 atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt gtctgacatg
     1501 gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc tcacaatcct
     1561 ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg tttaaaacat
     1621 caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga tcatacattt
     1681 acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt tacttccgca
     1741 accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa aaatccagag
     1801 atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat caacactttt
     1861 ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag ccaactgatt
     1921 ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta tcttccgaaa
     1981 gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac cgcctatatt
     2041 tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt attaaatcct
     2101 ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc ttgtccagaa
     2161 gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat cgattaaatc
     2221 atttattcaa aataaaagga cgagacctta ctaggtttcg tcctttttca taattactcc
     2281 gtaccatacc aaactattct gaaatttcaa ttgtttcaat cactacatca tgtacaggtc
     2341 ggtcttgtgg accacgttgt acatttgcga tttcatctac tgtgtccatc ccttctagta
     2401 catgtccaaa gactgtatgt cggaaatcta gccaaggagt tccgccacct ttatacgctt
     2461 caatgatttc tgatgggaat ccagcacctt ctagttgtcc aagcatatta gctggcacat
     2521 ttgtgttatt tacaataaag aattggcttc cgtttgtgtt tggacctgaa tttgccatag
     2581 ataaagctcc gcgaaggttg aacagttcac gactgaattc atcttcaaaa ctttcgccat
     2641 aaatactttc gccacccatt cctgttccag ttggatcgcc gccttggatc atgaaatcag
     2701 gaataacgcg gtgaaaaatc acgccatcat aataaccttt tttggaaagt tcaatgaaat
     2761 tttgaaccgt cttcggtgct agttcaggga ataattgaac agtaatgtca ccacggtttg
     2821 ttttgataac agcttttggt cctttttcac tttttaaatc tagttgtggg aatgccatat
     2881 tgtttcctcc taatatttgc atacttcata ataataataa cataatacct aacctctcgt
     2941 aaaaatacta gatttgtttt cttttatttt tcatcactag atacattcta taacttaccg
     3001 tttcgatttc gaattcaaac atttttgaga agcattctca attaaattct gctatagtgt
     3061 gaaaagggag gaatttttat ggaaatctat gacattacaa tcatcggtgc aggtcctgta
     3121 gggatgtttg ccgcttttta cgcaggaatg cgccaagcta aaacaaaaat cattgacagt
     3181 ctacctcaac ttggtggtca attagcaact ttgtatcccg aaaaatatat ttacgatatt
     3241 cctgggtatc cagcaataaa agcaagtgaa ttaatcgatc aattagaaaa acaattaact
     3301 acttttaacc atacattcca tttaaaagaa gaagttctct cgcttactcg tgaagacgaa
     3361 gttatcgaga ttactacaaa taaaggcatc cattattcca aagcagtcat tttagcttta
     3421 ggaaatggtt cattccagcc aagaaaattg aatttagata atgctgaatc atttgaaaac
     3481 cacggattgg attatttcgt gaatgatttg atgaagtatg ctggaaaaaa agtcgctatt
     3541 gctggaggtg gtgattctgc tatcgattgg acgttgatgt tagagcctat tgcaagcgaa
     3601 gtttacttga ttcatcgtcg cccggaattt cgtgcgcacg aacatagtgt atcacgcttg
     3661 aaatcttcat ctgtcaactt gcttactccc tatctcatcg atggattatc aggcaataat
     3721 ggggaactga cagatatccg gttaaaaaaa gtgaaaagtg acgagacgat tgatttgatg
     3781 attgattctc tgatcgtcaa ttatggattc tcttctaatt tagaacatct ttcttcttgg
     3841 ggacttgata gtaccagaaa tgccattact gtaaaatcag atatgtctac ttctattcca
     3901 ggcgtttatg cagctggtga catctgtacc tatgaaggaa aagtgaaact gattgctact
     3961 gggctcggtg aagcacctac tgcagtgaac aacgctttac actatattaa tccaaaggaa
     4021 cgaactcagc ctggtcacag cacaagctta tacgataaaa atcttcgtcc tagttgattt
     4081 tttatatctc tttgcatttc aagttaaacc cgaaagcagt ttcttgcatt tttttcatgt
     4141 tccgttaaca tgaaatagag aaagaaaaaa taaaggggga gcaataatat ggtcgtcaaa
     4201 actgagactt tagaagaaaa ggcaagagaa ctattgaagg aacgtggcgt aactgtagaa
     4261 gatatcgcac aacttgtcat gtttttacaa aaagactata tttctgattt gactttggcg
     4321 gattgtactg aaagtgtcaa cagcgtgcta acaaagcgtg aagtacataa cgccattatc
     4381 actggaatcc aattagatat attagcagaa aagaaaaaat tgatgagtcc gttgcaacat
     4441 attatcgaag atgacgaagg cctttacggt atcgatgaga tcctagcctt gtcaatcgtg
     4501 aatgtttacg gtacgatcgg ctttacgaat tatggttata tcgacaaggt gaaacctggt
     4561 attttaaaag aactaaacag ccacgatggc gttcatgtcc ataccttctt agatgatatc
     4621 gtcggagcga tcgccgctgc tgctgccagt cgattagcac acaaagaacc agaaaaaaga
     4681 agtcgcctta ctcaataatt caacaaaaaa agaaagaaaa cgtctattta gacgagttct
     4741 ttcttttttg tttgtttcaa gctatttttt cctaagaaaa gaaaaacagc aaaaaataat
     4801 tctaataata caaaaaataa gataaagcta tgcatgaaaa attcttctgg aagtacattg
     4861 atgattggat cagtaacggg atcgaataac cagtcatcgt tgttgaaaaa cgtttcatga
     4921 aaaaggataa aaaagcgatc aaatcctatc atcatgaaaa aaccgaaaac aactggcagt
     4981 aacatgccga tttgaaacgg acgaatcaac cgccaaagcc gatcattctt ttgcaaatat
     5041 ttgataaaca aaatactcgg gatcaaagtg atgaaaaaca cagcataatc cactaaaaac
     5101 aaaattttca cttcataaaa gtgatgggct ccgctagcag aaactgggaa atctggcaag
     5161 ctgaggatgg ttttgaaggg attattgaga taattcatta ggtgatcaaa atttttcagt
     5221 aacgtctctt gatctaaaga agtataatcc aaaatttgca aatgtccgat atcaaatacg
     5281 tatagtggtc gaaagttgat cgtcacacca actgccagac tgatcagtgt cagaaatagg
     5341 ctgaacaatc cagcatattc catccactgc catctcgaat ttttcatatt agaagctcca
     5401 ttcatccagt gattgtttca cataggtagc tgggataggc aacccaggta catcttcttc
     5461 ttttgtaaaa cctgataaaa cgagcaaagt atctatatta ttgcgtatcc cagcttggat
     5521 atctgtttca tagttatctc caaccatgat tacttcttct ttttgcagcc ctaatacttc
     5581 aacggcttta tccataatga tggcttctgg ttttccgatg tagatcggcg gcgtttgggt
     5641 tgctgtctct acaaaagaga tgactgaccc tgctccagga agaaggccgc gttcagtagg
     5701 tatattctta tctgggttcg ttccaatgaa agtagcacct ttttgaatag caagtgttgc
     5761 tttaactact ttttcatagg tcagataatt atctaagcct accactacat agtcaggagc
     5821 ctcttcttcc catgtaaatc cggcagctaa aatcaagtca atcaatcctg cttctccgat
     5881 aacatagact tttttccctt ttccatctgc tttcataaaa tcgattgttg ccaaagtagc
     5941 tgtgtagact gtctctggag ctacatgaat gtcaaactca tctgctagac gattagctac
     6001 tgtttctggt gacttagtcg tgttattggt aacaaacaag aaaggtaatt ttcttttttg
     6061 caattcttcc acaaatcgtt taccggccgg tatgggttgt gtcccacgat aaatcgttcc
     6121 gtctaaatca attaaatatc ctttatagtc cattcatcca ctactcttcc ctttttctga
     6181 tcgtaaatgc tgtttttgtt ttttctactt gtttaggttg ttcttgtttt tttacttttt
     6241 cagaggtttt tgctgactct gtttttcgat ttttaataac cggttttttg cgattagatg
     6301 cgactggtcc ttttttctca tcgatatacg cacgattatg gttttttttc ttttttcggc
     6361 gctgagttgt cttttctttt ttaccgccga ttctctcgat gacaaaatac gcacaaccaa
     6421 aattgcaata ttcgtaaaga tagtcttcca aggaatcgat tcgctgatct ggatgcgccc
     6481 gtcgattatc cgcttggaaa aatcctttta ggcgtagttg ttcataaccc caatctccta
     6541 cgatataatc atatcgggct agtacatcac tgtaacgttc tccaagcttt tctgcattaa
     6601 atccttcacg ataatcggac acaagccgat aatgtctttc acctatcaaa aaattactgt
     6661 catctaacag tgtcactagc tcgcctttga ctttttctgc tttaggctcc tcaacgattt
     6721 cttccaaagc agctgtcagt tcttcggtga ctgttgtttc ctgttcagct gatgattttt
     6781 tcttagcaat ttctgtttta tgctcaacgt tctgctgttc attttgtttt gcagacatgc
     6841 gttcaccacc tatctttttt attatacctt gtttttattt gattgggtag tgagctttca
     6901 agtaatctcc taccacactt cggataaatt cagggaacaa aaattcattt tttccagcaa
     6961 tctcaatcgt tggaaaaaat ggaacaaaca tcaaatgatc tacggtagta aaagtataat
     7021 ttttattcag atcgattggc ttgttcttcc aaaagacttg atggttaata gaatcatatg
     7081 tgattccatt atagaccatc tgcccaaaaa tcttaccgcg aaatcccatt cccttcatag
     7141 gatagttacg taaaaagtta cgattttttt ctatttctaa aactaagcgg atcagatcat
     7201 ttccacccaa tgtgacattt aacaagtgca tcggatgcgg caacgcagtg tgaagctgat
     7261 cctgattgac ccgccctttt ggaatttcag ttaaaaacag accactgttt aatattgctg
     7321 cttccgtctt tcctcgttct ttcaccgcct caaaggctac ttggataaac gaatgctcat
     7381 tgaagatatc tagactcaga gaaaatggca aatctgctac ttctttttct tttaataatt
     7441 catgcccacg ttctagataa ctatttattt cttgttcatc ttctgtaaaa gctgtcattg
     7501 tttcggtagg gatggctttg gctgaatgct taaggatttt tttctcttca tcgatggtta
     7561 aatggacttc accgacatac tgcccgtact ttccagcggc tgctatttgg acatcattga
     7621 ttacgtgacc ttctctgaat aaatgatggg tgtgcgatcc taaaataaca tcaattgctg
     7681 gtatttcttt tgcaatttgg aaatcatcct gaatcccgag atgactcatc aaaactaaaa
     7741 catccacttg tgaacgtaaa tcttcgacta attctggcaa aatatcataa ggattccgta
     7801 tgtcccagcc attcggatta taagtaagcg gaaatggagc agtaaatgca attagtccga
     7861 ttttcgtctg gtgtttcgtt gtaatgattt tatatggttt cgtccatgcc ggtggctgga
     7921 gcgtattttt atcgaataag ttatccagga tgatatcaaa ctcagcatga tcgtataaat
     7981 gattcaactc ttctttggaa tttcctacgc cttcattgtt tcctatcgtc gctgcatcat
     8041 aaccaacttc attcatcagc aagatatttt cctgtccatt cgttgcttct gacaatggat
     8101 gccaacgatc cacaaaatca cctagatcta ctgcaatcgt tgtcgttttt tctttctttt
     8161 cgttttctcg tttacgttga tccaaaaacc ttctgatcct tggccaattt tctaaatgag
     8221 aatgcagatc attcgtatgt aaaatcacta ttttttccat gtaccctctc gcctctcttt
     8281 atctttatca taccataggc agctttttct tccattgaaa caagctctat ttcagagttt
     8341 ttcctgcctt gaagcaaatt gtataagtac aatattcgat aaatcagtct ggctaaaaaa
     8401 tcaggaacat tcatttttac agtactataa aaataaaaaa aacagacccg aaagtctgtt
     8461 gtcgtttcat tataatcaga cacatggcgg aacctgctta gtgctgcttc cttccagacc
     8521 tgacacgctt cacaagccca ccattgtcct agcctttcgg caaagaatag tataccttat
     8581 ttttcattcg ttgaccagtc tttttttaat ttcttttttc gctggcggag ctttcggaaa
     8641 aagtcagtca aaatcatccc acattcgtct tctaaaatgc caccttctac atatgcttgg
     8701 tggttaaacc gctcgtcctc caaaagattc atcaaggtac cggctgttcc gccttttgga
     8761 tcatacgcgc cgaaataaac ttctgctaca cgagacaaaa tcatcgcgcc gctgcacata
     8821 ggacaaggct ctaaggtaac aaaaagtgca gcatcttcca agcgccagct tcccactttt
     8881 tcacaggctt ccttgattgc aagcatttct gcatgtgtag tagcatcttg cgtggtttct
     8941 ctaaggttat atcctcttcc aatcactttt ccatccaata caaccactgc tccaatcgga
     9001 acttcatgaa gcatttctgc tttctttgct tctgccaatg caaggcgcat ccatttttct
     9061 ttttctactg catcaagatt tgtatttacc aattaccctt ccctgccttt gttttttctt
     9121 gttttacttt agcatatgat aaaaaggctg taactaactt ttttttctat ggtaaaataa
     9181 atacaagagg tgaacaagat gaaaatggat aaattacttg ctctttaccc acaagcacag
     9241 aaaaaaaact ctccagcatc agaagcggat atcctttctt ttgcaattga tgatgctttt
     9301 atctggatca aacaagattc attatctcaa caagaaacca gccttttgaa agcgttattc
     9361 cctgtaatca acgatcaaaa aaaacaccca tggtatcgct atctttttaa agatgcttct
     9421 tgtgttcaag aaaaaagctt tcgtttgatc cagcttcacg tcgaatcaaa aggagaattc
     9481 ttaaaaaaag aatggcaaga tactattttg gaattatttc gctattctgc tgatttcttt
     9541 ttttatacag acacagatgc gctcttagtc gaacaaataa accgtgatta tcttgatgct
     9601 actgaaatca acggtatttt tctatcttta gatgctgatt ttgatacgac aacgtctgta
     9661 ttcgttggtt ctttccattc acctggcacc gacctcgtcc ctttgtttgc tgaagaaaga
     9721 aaaatttttt taacagagca aaacaatctt tatactcaca gccgtacttt ttctatgcaa
     9781 gatgtggctt tacattacta tacaaaagat actgtaaaag atagtaagtt gatcaaaagt
     9841 tacatagaac tgatcaaaca aaatgaaatg gaagtgatca ttcaagaatt atggaatgaa
     9901 ttaggcaatg tcagctctgc tgcaaaaaca ctgtttcttc atcgtaatac tctaaaatat
     9961 cgaatcgaaa aatttcaaga acagtctggt ttcaatctaa aaaaagccaa cgatttactg
    10021 ttttgctatc ttttattgct tcatgaacat aattaaagat agattaaagg ggttgtgaca
    10081 ataattttgt cacaacccct tatttcgaat gaatagtgtt caaaagctag tttctcttac
    10141 atcatttttt caacaaaatg cattagccgg tcttttacat cttcggttga atctttgatt
    10201 gaatcaccat aaacagagat tttctttttt cctgatttga tttttgctaa cattcccatc
    10261 aaagaatcca agtcactatc tgaaagacca tctacgattt ctagtaattt ttcatttcca
    10321 tcaaatttgt catagacgaa tttttttact ttcgtccgag tagtcatatg atggatcttt
    10381 tccattactt ttcccgatac aacgactgct gtcgctacac tggcagcggc tgctacgctt
    10441 aatccgattg ttacttttgt tgatgttttc ataagtatca gctccttttt atctatctcc
    10501 atcatagcat agacaaaaaa ataaacgaac taatccactc agtgaaatct caatccttta
    10561 gggaaaaagt tctttactgt tccttttact tgtttcccaa atccgacagg tatcttatct
    10621 aatactagca aaacccatcc ttgattccca tcacgttgaa atgtttctcc tgctgtgtaa
    10681 gattgccatt ccttttgagt gatcggcaag caaggtatat tctctatctt ttttgtagca
    10741 agcgctaatg catagctagg ttcaaatcga ttctttttga aatctcctaa atgcaaacct
    10801 gtacgtacga cttttaaacc atcgagagaa ggggctaact caggaacttc ccaaagatgg
    10861 tcgttgaaca ctaaaagtct tccagtagct tcataatgaa aatcatttga aaactccgtc
    10921 cagagttttt cctgctcttt tgtcatttgc actttacttt tttttcgtgt cttcttttct
    10981 ttatgcatct gattttgtcc atgaaaagtc agctttgcaa caaaatgtcc ttcaccctga
    11041 tctttatgag gccatatacg tattgttttt tccaaaccag ctacgcttcc ccattcagaa
    11101 cgtcctgaac taacggattg agtcaaagga atctcctcaa tcgttactgg ataattttct
    11161 acaagccagc taatgatctc ttcattttct tctggtgcaa acgtacaagt cgaataaatc
    11221 agttgtcctt tattttttag catttttatt gctgaagaca aaatctcttg ctgacgtttt
    11281 tgacagtata gcggagattc ttctgtccac tcttttattg catttggatc ttttcggaac
    11341 attccttctc ctgaacaagg agcatcaacc acgatccgat caaaaaagcc cgaaaaatga
    11401 gggacaagtt ctgcgggtgc atgatttgtc actattgcgt tcgaaactcc ccatcgttct
    11461 atattttctg ataaaatctt cgcacgtttc ggaaagatct cattcgtcac taataaccct
    11521 ttccccttca tttgagcagc taattgggtt gatttaccac cgggagccgc acacaaatcc
    11581 aaaacttttt caccgggttt tgctgcagca gctgttccaa cgatcattgc acttggttct
    11641 tggctatatt catacccagc ttgatggagg aaagactttc cattgacagt gccaagaaat
    11701 ccctcattgc tgtagggagc gggttgtaat tcttcgctat ggtaagtttg gaccatgtcc
    11761 aatcctgccg gttttagtgg attccaccga aatccttttt tcacgcttcc ttgttcaagc
    11821 gcagaaaaaa aatcggatgc ttcttcgcct aaaagaagac gatatttttt gataaattgc
    11881 tgtggtaacg ttgtcgcttc ctccttcatt ccaatgtttc gttagacatt ttacaatctt
    11941 ttttacgaaa aataaaggtg gctgatctac tttttcatgt tgctgcctaa ggtgactgtt
    12001 cctttaagat attttccaag tattcatccg tcacctcgtt tttatcccaa acagcgttca
    12061 tcatttcaag aaaatcttga gaagaatcat ctgagcgcaa acttcccaat gcattaagcc
    12121 ataccaattc aagcgacttt tcttctttga tcttcgtgtg acctttgaca tatgagaaaa
    12181 atggccgctt aaatagcgaa tccatgtcta aatcagattt tgcaatgatt tcacgaatac
    12241 tttcatggat agaaaaaaaa tctttcaata agtagacaat gcttaaaaca tcatcggaac
    12301 tgcattcagc taagtagttt tcaaaataaa cagtgatttc ttctaatatc ttctctttta
    12361 acccgctgat tgatttgaaa tgtgtataaa tcgggtatgt agaacaaccg cagaactctg
    12421 ctaactctct tgcagtaatt gacttcattc catattcttc cacgtatacc atacttttga
    12481 acagcaaata ttctttacta taatttgtta ctctccccat cattcttacc
//
LOCUS       DAZULY010000073        12491 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000073 DAZULY010000000
VERSION     DAZULY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.074"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1451
                     /locus_tag="ACG81A_001293"
     CDS             <1..1451
                     /locus_tag="ACG81A_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFS5014664.1"
                     /translation="AKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKNGFMSARD
                     SFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMAIVNNVTN
                     SIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTFYNGTNGH
                     VREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPMFEEDSAY
                     SKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATKQELPNLN
                     GRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPIKDWEKKG
                     LVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYNFEFIRNI
                     RGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKEPVRRKTD
                     GFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            1486..2568
                     /locus_tag="ACG81A_001294"
     CDS             1486..2568
                     /locus_tag="ACG81A_001294"
                     /inference="COORDINATES: protein motif:HMM:NF028182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage abortive infection protein"
                     /protein_id="HFS5014665.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            2612..3784
                     /locus_tag="ACG81A_001295"
     CDS             2612..3784
                     /locus_tag="ACG81A_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS5014666.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            3771..4475
                     /locus_tag="ACG81A_001296"
     CDS             3771..4475
                     /locus_tag="ACG81A_001296"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFS5014667.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            4480..5640
                     /locus_tag="ACG81A_001297"
     CDS             4480..5640
                     /locus_tag="ACG81A_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS5014668.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            5716..5994
                     /locus_tag="ACG81A_001298"
     CDS             5716..5994
                     /locus_tag="ACG81A_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HFS5014669.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            5975..6346
                     /locus_tag="ACG81A_001299"
     CDS             5975..6346
                     /locus_tag="ACG81A_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HFS5014670.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            6362..6766
                     /locus_tag="ACG81A_001300"
     CDS             6362..6766
                     /locus_tag="ACG81A_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014671.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            6763..7173
                     /locus_tag="ACG81A_001301"
     CDS             6763..7173
                     /locus_tag="ACG81A_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014672.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            7234..7821
                     /locus_tag="ACG81A_001302"
     CDS             7234..7821
                     /locus_tag="ACG81A_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFS5014673.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            7894..8361
                     /locus_tag="ACG81A_001303"
     CDS             7894..8361
                     /locus_tag="ACG81A_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HFS5014674.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            8407..8715
                     /locus_tag="ACG81A_001304"
     CDS             8407..8715
                     /locus_tag="ACG81A_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014675.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            8910..>12491
                     /locus_tag="ACG81A_001305"
     CDS             8910..>12491
                     /locus_tag="ACG81A_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS5014676.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNP"
BASE COUNT         4486 a         1847 c         2723 g         3435 t
ORIGIN      
        1 tagctaaata tcaaaaattt atcacacctt tcattttctg ctatcaaaaa gaggacgacg
       61 aggtgttttt tgatgaaatt ctaaatagta ttgcgcgagg cggcgggaaa aatgggttca
      121 tgtcagcaag agactcattt tttattagcc ctctctacgg tgtcagaaat tatgatgtaa
      181 cgattacagc gaattctgag aagcagggaa aggtatcttt taaagaagtt tatgaaatgg
      241 tacaaatgcg caaactggaa aaacaatttt atttgactaa gatggctata gtcaacaatg
      301 taactaattc gatttttagt tataggacaa acaatcctaa gactatggat agtgcaagag
      361 atggctgctt agaatttgat gaaatccatc agtttgaatc ttcagaccta gttgatattc
      421 agcgtagtgg gctagggaaa ataaaacatc ctcgaacttt ttataatggt acaaatggtc
      481 atgttagaga aggtttttat gacaaaatgt tggaacgagc acaaaagatt tttagtggag
      541 agaacaagaa tgatcgcttg tttcccttta tttgtaaact ggataacatt gatgaaatgg
      601 ataatcctaa aatgtggtca aaggctaatc caatgttcga agaagattca gcatattcaa
      661 aacgtctgtt ttctacagtg atgaaagaat ataaaaaact agaagatgag ccatcaggta
      721 ggcgtgaatt tgttgttaaa cgaatgaatt ttacagaagg tgatgcagaa tcagatatta
      781 cgactcatga aaaattaatg gctactaaac aagaattgcc aaatttgaat ggtcgttctt
      841 gtgtggctgg atttgattat gcaagtattc gtgattttgc aacagtgggt ttgcttttta
      901 aaattgatga taaatttatc tggcagcaac atagttttgc taggaaaaag tttctagata
      961 cattcaaact gaaagcgcct attaaggatt gggagaaaaa aggtttagtg acaatcgttg
     1021 atgagccatc aattgatcct cgtcatttaa ttgattggtt aaatgaaaaa agaaaaatcc
     1081 atcaaataga aatcgtttgt gccgacggat ttcgtatgga tttgctgaaa cctcttctag
     1141 agaaagaatc atataatttt gagtttatca gaaatataag aggtgtgcag tcaaaagttg
     1201 ctcctattat tgaagatggt tttgcaaatg aacggtttat ttttggtgat gatcctatga
     1261 tgcgttggta tacaaataat tcgtttatca aagaagacaa attagggaat aaaacatttt
     1321 tgaaaaaaga gccagtaagg cgtaaaacag atgggtttca tgcttttttg gcagctttat
     1381 ataaacgaga agaaataact gatatggatt atgaggaagc atttgatatg ttggatgaga
     1441 ttgaatttta aggtatactc cttattgaaa ggggtagtta tagaagtgaa aaagagatat
     1501 tttctatttg ttacaatatt tataataata gcaatttgtg ttttgacagt atttttacca
     1561 caaattactg agtatttaaa ttatcggata tttaccacaa aagtagatga tagcgttact
     1621 agtttaagta ggctagaatt tataaaaata tgtgcatctg tgtttggtcc tattttgact
     1681 gtaatggtat ttgtgaatac tttaaatatg cagaaaaaaa cagagcaaaa gcaaaatgag
     1741 cttgaagtaa agcaagctaa ggctgagaac gagagagcaa aaaaagagct gttaacacag
     1801 atagatcgtg agttctattt tttattaaat atgtttattg aaatacaaaa agatcaaaat
     1861 gccaaacaag ctgttattgc tttacagaag ttggcaattt ttaacgaaaa gggttttaat
     1921 caagactatg catttacttc taaagattta tcagatagtg aaattgggag tacaattgaa
     1981 caatcaggac cgctgaaggg cacagttgaa tatataaaat ttgaaacatt tgatgtaagc
     2041 aagtcgatgg ggaattatca aacaaaaaca aaagacgttg ctacaagttt acacgatcag
     2101 aaagtagcta tattgaatga acaatatgaa aagatgcaaa tatatcttgg acggtacttc
     2161 aaaatgtttc accgaatagt taaaacatta aatgagtatt atgacgatta taatgatttt
     2221 gatgtgaaga ggtatacaaa atatataggg acattaagaa cacaaatttc tccagctgaa
     2281 ttccaagtga ttttattcaa ttctctatat ataaaacgag gttttggctt aggaattcaa
     2341 cttattggaa gtggtttttt tggagatgat tttgattttg aaactaatca acattttgaa
     2401 acaagtatca atgaacagtg gtttctatca ctatcaactg ttgacagtga taactctata
     2461 aagcgacaaa aattatctga atatattaaa gaattaggat ccgtagaata taaaaaaata
     2521 gctaattttg aaagtttata taaactcttt aatgaaacta agctctagaa atagggcttt
     2581 tttatttttc tttgaaaggt ggtgaaaaca tgtgagctta tttgatatct ttaagcagtc
     2641 aataagaaat gaagagcctt ctgagtggat accggatttt gtatatggag aggaagtatc
     2701 tgctcgggca tacctcaaaa ttatggcaaa aaatactgtt ttagattttg ttgcaagaac
     2761 tatgtcaacg ttagaagtta agtttaaaaa taaagatggg acatcagact gggattatat
     2821 tctaaatgtt cgacctaatt ctgatatgtc agcagctaca ttttggcaaa aattttttta
     2881 tcggctctta gatgacaacg aagtgttagt aatatttaca gatgataatc aattgctgat
     2941 tgctgatgat tttagtcgaa cggagtatgc cgtgtatgat gatgttttta caaatgtgat
     3001 ggtaaaaaat tttgtctttg aacgttcatg gaacatgtct gacgtcattt atatggaata
     3061 taataacgaa gaattagaaa ggtttacacg aggtttattt gaagattacg ccgcgttgtt
     3121 cggacggatt ctagaagttg caatgcgtaa caaccaaatt cgtgggtctg tatccattga
     3181 ttcaacggga acggcgaatg atgaaaaagg aaaagatggt aagactagat ctcaaagatt
     3241 acaagaatat attgatagga tttggaactc ttttaaaact aactcagttg caattgttcc
     3301 caaagtgaaa ggctttgagt atgaggaata caccaataaa caaggcgtat cgaatcaatc
     3361 gctagatgaa ctgaataaga tgaagtcgtc tttaattgat gatgttgcca acgccatagg
     3421 agtacctacg gcgcttattt atggtgaaaa agctgaactg gattcaaata ttaaagcttt
     3481 tcgtaaattg tgtattgctc cactagttaa aaaacttcaa gacgaattgg tggcaaaaat
     3541 aattagtcgt caagagtaca aaaacgggga acgtatagaa gttaaaaaag ttctccaaaa
     3601 agatctatta gagtatgcta ctcaaattga taaaataaca tctggtggtg cgttttatat
     3661 tgacgaagtt cgtgatgaag ttgattatga tgagttgcca aataatgaag gaaaaattcg
     3721 tattcgtacg aaaaactatg aagaaactgt gaagggaggt gagaacgaca atgccgaaag
     3781 ctaaaaaagt accatttcag tttactaacg aaattcagaa tggtaagcac attctcacct
     3841 tgagtggaaa tgtacagaaa aaatattggc gtgatgatga tgtcataaac gctaaagata
     3901 ttcgtgactc tctagatact gtaaccgatg atattgtaat caagttgaac agcccaggcg
     3961 gtgatgtgtt tgaagggatt gagatttaca attatttaaa agatcaccca tcaaatatta
     4021 ctgtagaagt tacagggctt gccgcaagcg cagcaacgtt tattgtcgct ggtgccaatg
     4081 atgtgattat gaatgttggg acttctttga tgattcatga agcttctaca tttgcttggg
     4141 ggaataaaca ggacatccaa aaaacgttaa acgcattgga aacgatcgat gattctatct
     4201 tagctattta ttcagataag actggccaat ctgttgataa gttgcgtgaa tggatgaatg
     4261 aagaaaaatg gtttacagca gatgaagctg tcaaatatgg atttgccaat tctgtgaagc
     4321 gtgaagaacc acaaaatgaa cctcaaaata ttgcagcgat gattcaagat gctgttgccg
     4381 ttgcaatagc gaatttaaag gaacctgtta ctaataaaat cgaacaagaa tcaaaacaaa
     4441 aatcattaat cgcacgattg cgaaaaggag aataaagata tgttaaaaat tacagataaa
     4501 actgcagatg caaaaaaggc atttaatgct atttcttcga aagaagatgc aacacccgaa
     4561 caaattaatg ctgctctaga agaatatgta accgccattg cagaagatgc cggcaaacag
     4621 gtgcgtgctg aatatgaaga attgaaaaat gtgacggaca accgtgtatt agaggctcgt
     4681 ggaattccta cactaacagc agaagaaaca aagttttata atgaagccgt aaaatctgga
     4741 ggtttcgatt ctgacgtagt ttggccagaa acgattttgg aacgtgtttt tgaaaatctt
     4801 caaaacgatc atcctatttt aggcctcatt aactttacac caactgtagg tcgtgtcaaa
     4861 gtaattagag cacgtcgtaa aggtgttgct gtttttggac cattgcataa agaccttgaa
     4921 ggccaactag atgctgaatt tggtgcaact gaatttgttc aacttgcgtt gaccgcattt
     4981 ttcttaattt ctaatgatac cttagattta ggaccacgat ggattgaccg ttttatcaat
     5041 ctttctttaa gtgaagcagt acgtgatgtt tgggctaaaa aaattattac aggttctggt
     5101 aaagatgagc caattggatt gcttaaagat ctagatggtg cggtcacaag tggtgtatat
     5161 ccagataaag ctagcgcagg gacattaact tttgcgaaag agaagattgt tcttgaactt
     5221 gcaggggtga tgaaaaaatt atctaaatac acatacaaag tagatggtga cgatgaaggt
     5281 gaagtgaaat accgttctgt tgctggacga gtgtatctaa ttgttaatcc tgttgattac
     5341 tgggatatcg tagctagagt tacatttgca aatttaaaca atgtgtatgg gactacgatg
     5401 ccatttatcg ctgttgatca tatcatcgaa tctgtagacg taccagaaag taaatgtatt
     5461 gcatacgttg ataaagagta tgaagctact caatcaagag ctgaaaaagt ctatgtatat
     5521 aaagaaactt ttgcaatgaa acgcgcaact ttgtatgctg tcgacatgct tggtaatggc
     5581 tatccaacaa ataatgatgc agcacaagta tatgacttgg actttacagc gggggagtaa
     5641 tgtcccctgc aattggtgag ataacaccga cgactgatgg ggcagtcatt aatctttcgt
     5701 aaatggggtt agagtatgaa cactgaagaa tttataaaag aatataaggc ccgatttcgt
     5761 attttccata cttccgaaga tgaggatatc gggaaacagt tagagagcgg gtttgccgat
     5821 ataaaatctc taatcggcag ttttgatcct tccacttatg agaaaggaaa agaattagtt
     5881 tatgaacgta ctcggtatct acggaacgaa tcactagagt acttttacga caattttcaa
     5941 acaatgatta tggatgcatc aattgatctg gcaggtgctg gatatgccaa ttaaatcaaa
     6001 ttataagaag cctaaaattg tagctggtga tctaaatacg ccagttactt tttttgagtt
     6061 tcaacctaat aagggtccag aacctggtga tgaagaaaaa aaggaactgt atttttgtac
     6121 agcactagtt tacaacccat caatgaaaga tcgtgacatt cttggcacaa aagaaggcgt
     6181 tacgataaaa atacgggatc cccatcaatc ttatattcct actaacaaac ataaagtaag
     6241 gatattagat tatcgatatg gagataaagt ttgggaaata atcgatgtct caccggattt
     6301 ggaagaaaat tcaattgtaa agatagtatt agaattaatt acttaaattt ggtgataaat
     6361 gatgagtaac gtaaagtttg aaggcgttca agaaacaatc caagctttag aaaaaaagta
     6421 tggagaaaag aaaactaaga cattaacaaa aaaagcaatc aatgttggcg ctgaaaaagt
     6481 agaaaaacag ttgcaaacgg acatgaccgt ttttaaagac aaaggttata cgatcgctga
     6541 ggttgttcgc aagaacgcta cgtatagaga ttacaaggca gaagctgaaa tcggttggaa
     6601 tggtccacat caacgatata gaatcattca tttgaatgaa tggggatata cacgaaaggg
     6661 caaacaaatt agaccgagag gatttggtgt aataacaaaa tctcttaaga attccgagct
     6721 gttgtacttt gatgctgtag gagaggaggt taaaaagagt ttatgaaaga tatgcttaat
     6781 atcatttacg aggctctttg tttgaatgag tatattcatt caatgaccta caacgagaat
     6841 accgagcaat atcgtattaa attttatgag caacctgaaa ccgccgataa aacaggtcca
     6901 atgatcacaa ttcgtcctgt agatacacct aacgaagcgt accacggaag taacaaagag
     6961 ctttctgtgg agcatatgat acagattgac gttgaatcga aatatagagc aacatgcaag
     7021 cagattcaat atgagataaa aaaagagatg aaaaaactag gctttggcca aatttccggt
     7081 caagggttag atgaatactt tactgaaaca aaccgctttg tagacgcgcg tcgttacgaa
     7141 ggaaatacaa aaatttacga taccgaatat tagaaattaa gacacgaaaa taacgtgtct
     7201 ttttttatta cccaaaatta ggaggaaaat tttatgactt tagtaggatt caaaaaaatg
     7261 acaatcgggg tctttgacag cacagggaag attccagcag caaatttata tgtaattgaa
     7321 ggtgaacaaa acaaaggggc gactgtatct gctgaaatca gtgggctatc aaaagaacca
     7381 tcaaaagttt acggatcgaa catcgcttac tatgtctctc aaaaaggaac aggcgatgtt
     7441 tcagctactt ttggattgtt agatttacca acagaagtaa atgacaaaat tcttggttac
     7501 aaaaccgata caaacaaaat cagcttcttg ggagaagata ctgagccacc atattgtgca
     7561 attttgatgg aatcggagga tttgaacggc gatacagcaa tgcttgcgat gttcaaagga
     7621 aaattcagtc gtgaatctat taatcttaac actacgacaa atgaagcttt cgaaccagaa
     7681 gcagaagaat atgtattttc agcgattgcc aatgatgttg aaggtgatgc taaaggccaa
     7741 acagtcgcaa aatatgttgg agacgaagaa gcttctatta ctggtttgaa agagatggta
     7801 tttcctgcag cgggggagta actagccccg ttgttggagc aatcactccc actattaatg
     7861 gggcaacagt tgaattaagt taggaggata actatgccag atacatttaa aatctataaa
     7921 aaaaatggta ctgaatttac aaaagtcgca gagggtgaaa gtccactttc tattactgga
     7981 attgcagcaa atactcaagt tgctaaagga gattatcaag ctacgcgtgt ggtcggaggt
     8041 caagaatcag taaaagtaga tattcctgca ttcaccactc ttccaatttc agttactggt
     8101 gtgacgttgg ataaaacgac agctgaagtt gaacaaggcg gaaccttaaa attaactcct
     8161 actgtaacac cagcaaatgc aacagacaaa acaggaagct ggagcagttc gaatacagct
     8221 gtagctacgg ttactggagg caacgttaca gtaaaaacag acgctgaagt tggcggaact
     8281 acagaagttt catttactac tacggatggc ggtaaagtag caaaatgtac tattacagtt
     8341 attgaaaaag cagcgggcta gtcttcggac tagctttttt atttggaaaa taatggagga
     8401 atctaaatgg caaaagtaag aattgaattg aaagaagaat caggaaagaa agtctatgaa
     8461 aacatagaca caacagggaa agattatcga aaagctttag aaacaatcaa aaagctaaat
     8521 gaagacggag taatgatttg gaatcgttta gacatttatt tggattttgc tgtaggtatc
     8581 ttcagggata gcaaattgac agctgaacaa attcttgatg gcttaccatc tgagaaggta
     8641 atgagcacat tggacgacat cctaggagaa gtaatgggga ttgaaagtaa tccagaccca
     8701 gacgcaaaaa agtaacaccc gaagaagcag aagaaatgta cttaaaccta tgcagagagt
     8761 tagtgaaagc gggatggtca ctatctgaca tagaagataa ttcctttgaa atgatgatga
     8821 aaattgcgtg cacttcgcct aagaaagaga aacaaaaaga agttgatctt aaagacttct
     8881 tgaaatccat gtagagagga ggtaaacata tggcaaatgg aaaaccttta ggcaatatga
     8941 ttatcaaact agacttagac agttcagctt tttcaaaagg tctagccgga gctaaaaacg
     9001 ctgttaacca tcaaatgaaa gcaatgaaat cacagatgca agtaatgaac tcttcaggaa
     9061 attctctcgg tgctttacaa gcaaaataca atggtttagg cggagttcta caagcaaatg
     9121 aaaagcaagt agaattgctc actaaggctt ataaggatag ttttgactct aacggaaacg
     9181 ctactgcttc gactgctaaa tacgctaatc aattgaatca agcaacagct agatcagcaa
     9241 gttacgaatc tcagatgaaa acaactgttg gacagattgc tcggatgaaa gtggaaacgg
     9301 aagggtttac ggggaaacta aaggctaatt ctgatgtatt gattaattct gggaaaaaaa
     9361 ttgaagcctt tggtaaagga gtttctggga taggatctgc cctaactgtt ggagtaacag
     9421 ctccaatagt ggcaggcgca actgctgtta ctagtgcagc tatttcatgg gaaagtgctt
     9481 ttgcaggtgt aaaaaagaca aatgatgaag ttgtagactc gaatggaaac gtagtttatt
     9541 cctatagcga cttagagaaa ggtctgcgta atcttgccac ccaattacct ggtagccatc
     9601 aagaaatagc aaatgtagcc gaagcagccg ggcaattagg tataaagact gaaaacgtag
     9661 ttagcttcac taaaacgatg atagacatgg gtgaatcgac taatatgtcg gctgaaactg
     9721 cggcaacttc tttagcacga tttgctaata ttacccaaat gtctcaaaaa gactttgata
     9781 aattaggatc agtcattgtt gatctaggta acaattttgc tacaaccgaa tcagaaatca
     9841 ctgaaatggg attgcggtta gcgggagctg gtaaacagat tggaatgagc caaggcgaaa
     9901 ttctaggatt tgctactgct ctatcttctg ttggtgtcga agctgaagcg ggtgggtctg
     9961 ccttttcgaa ggtcatgatc cagatgcaac tagctgtaga aaaaggattt ggcgcttttg
    10021 atcagctgaa acagatggca gaagaacaag gtgttccgtg ggttaattta gtaaatgctg
    10081 tgagagacgg tggaaaatcg ttaaaagcag tatcagagca aatgggattc acttcatctg
    10141 atttaaagaa aatgtataaa gaagcggaca attcgaaaag ttcgcttgaa aattttgcga
    10201 atgtggcagg tgttacaagt gaacaattcc agaagttgtt caagagtgat ccttcagaag
    10261 caatcatcaa gttcattcaa gggctgaaag attcagaaaa acatggtact tcagcaatca
    10321 aagttctgga tgacatggat ataaaagaag ttcgactacg cgacagttta ttgagagctg
    10381 caaatgctag tgacatattc aaaggagcaa ttgaaaaagg aaacggcgcg tggaaacaaa
    10441 atacagcttt aacaaatgaa gctaataaaa gatacgaaac aactgaatca aagctaaaga
    10501 tattgaaaaa cgaagtgatt gatacagcaa tcgatcttgg aggtccattt gtagacgcgt
    10561 tgcgtgatgg tctaaatgta tcgaaaccat tgataaaaaa cttagggaat atggcgaaag
    10621 ctttctcagc tatgagtgat gatcaacaaa gaaacattat caaatgggtt ggattagcag
    10681 cagcagctgg tccagcgctg aagcttttag gaactggtac ttcagtaata ggtaaagtta
    10741 cttcaactac tggaagattg acgaaaagcc ttgttgattt agcagcgaaa gcagcagaaa
    10801 agaaggctat ggatgccttt gcatcgtcgg taacaacagc tggtgctgca gctgcaaaga
    10861 cagcaggagc tggaggtatt ggcgggttag tccctatgat gggaaaaaca gctagtgctg
    10921 caagtactgc tgcgggcgcc agcggaatag gcgcaatgac aacgtcacta ggtttgttag
    10981 gtcctgctct acttggtatt gttggcgttg gtggcgcact ggcagttggc tatggagcat
    11041 ggaaattatt tggtgaagaa gcttggaatt cctcgcaaag agtaaagcaa tggggaacag
    11101 atgtcggtga ggagatagat agtactcttg acggagtaca gaagaatatt gaaggtgcaa
    11161 atggtcaatt cggtcttctc aaagagggtt ttactgttga tgatgcaaat aatatggcgg
    11221 ctaatttcgc aaccgctgga acatctttgg aaacatcttt aacgggtaga ataaaggcat
    11281 tagacgatgc actaaaaggg ctacctcaat cagttcagga tgccatgaaa gaagtagctg
    11341 aatcagaaga agaaacctta gggcaatctc tttctaaaat aaaagacaac aatgaagaaa
    11401 taaaaaaaat cagagaaaga gccgcaaatc agaacagaga cttgactgtt tcagaactac
    11461 aacagataca ggatctaatg aaaggaactt ctcaagcgta tgtggatacg ctcgatgttt
    11521 ctgctaaaga cagaaaaaag atactatcag ccatgaatgg tgatgttgca caagctacag
    11581 aagaagaagc gaagacgtgg ttacaatctt tagcgaaaca aaaagctgca actaccgaaa
    11641 attatagaca aatgcttgaa gaaaaaaagg cggctttaaa ggaaaaggga gtaagcaaag
    11701 aattcattga tgcattagaa gatgaaatga atgattacat caaaactaca aatcaaggat
    11761 tcgatagcca aatagctgct ataacaaata aatatccaca attgattaac gaagtgagtt
    11821 tatcaaatgg tcaattattt agcaaaacgg cagctgctat ggacaatact ggtcagtata
    11881 caaaagtttt tctcgaaagt aatgaaaaga ttttagccca agcgaagaaa acatcggatg
    11941 aaatagcaga taatgccgaa aaaaatgcag aacaacttgg tatggtagct gatgaggcta
    12001 cgcatgctgg atcaacttgg aatggcttag ttttagacga aaaaacagga aaagtaaaaa
    12061 ctaatgtaca agaagtagtg aatgaagcaa cgaaggattt cactaaatgg aatgaattga
    12121 aacctgttgt tcatgatgcg aaactaacaa cgaatgcaaa agatgtcatt tccatagcgg
    12181 cagtacaaaa tggctattgg gatgatatgg aatggaaaga aaaagaagca ttattagaag
    12241 atgagtgttc taaaaatgta tacaaggctt tagaaaatgc tggtaaatgg aatgagttag
    12301 atattccttc taaagaagca atcctaactt caaatactcc tgaagttatg ggtgaaactc
    12361 tcatgaattt gggattatgg gatacttaca aacttcaagt gaaagattta gatttgaatg
    12421 cgtataaagt atacgatgct atcgatggat cagaagaaaa attaaatagt tgggctcaat
    12481 tagataaccc c
//
LOCUS       DAZULY010000074        12440 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000074 DAZULY010000000
VERSION     DAZULY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.075"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..670
                     /locus_tag="ACG81A_001306"
     CDS             <1..670
                     /locus_tag="ACG81A_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014677.1"
                     /translation="FPWFALQNTPLNIIQAPWRFMIFSTLFISFSVSLFISPQLEKFS
                     YTRRRNFILLLILAISILNYSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDYVPIGY
                     KKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNS
                     KNATVLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIR
                     TSHE"
     gene            complement(937..2106)
                     /locus_tag="ACG81A_001307"
     CDS             complement(937..2106)
                     /locus_tag="ACG81A_001307"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS5014678.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            2310..3485
                     /gene="mltG"
                     /locus_tag="ACG81A_001308"
     CDS             2310..3485
                     /gene="mltG"
                     /locus_tag="ACG81A_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFS5014679.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            3621..4100
                     /gene="greA"
                     /locus_tag="ACG81A_001309"
     CDS             3621..4100
                     /gene="greA"
                     /locus_tag="ACG81A_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFS5014680.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            4269..5000
                     /gene="liaF"
                     /locus_tag="ACG81A_001310"
     CDS             4269..5000
                     /gene="liaF"
                     /locus_tag="ACG81A_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFS5014681.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            4997..6064
                     /gene="liaS"
                     /locus_tag="ACG81A_001311"
     CDS             4997..6064
                     /gene="liaS"
                     /locus_tag="ACG81A_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFS5014682.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            6071..6703
                     /locus_tag="ACG81A_001312"
     CDS             6071..6703
                     /locus_tag="ACG81A_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5014683.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            6843..7508
                     /locus_tag="ACG81A_001313"
     CDS             6843..7508
                     /locus_tag="ACG81A_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFS5014684.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            7536..7829
                     /locus_tag="ACG81A_001314"
     CDS             7536..7829
                     /locus_tag="ACG81A_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFS5014685.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(7897..8844)
                     /locus_tag="ACG81A_001315"
     CDS             complement(7897..8844)
                     /locus_tag="ACG81A_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFS5014686.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(9022..9825)
                     /locus_tag="ACG81A_001316"
     CDS             complement(9022..9825)
                     /locus_tag="ACG81A_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFS5014687.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            9971..10885
                     /locus_tag="ACG81A_001317"
     CDS             9971..10885
                     /locus_tag="ACG81A_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5014688.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10933..11403
                     /locus_tag="ACG81A_001318"
     CDS             10933..11403
                     /locus_tag="ACG81A_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFS5014689.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            11488..12096
                     /locus_tag="ACG81A_001319"
     CDS             11488..12096
                     /locus_tag="ACG81A_001319"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFS5014690.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            12230..12430
                     /locus_tag="ACG81A_001320"
     CDS             12230..12430
                     /locus_tag="ACG81A_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5014691.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
BASE COUNT         4202 a         1907 c         2460 g         3871 t
ORIGIN      
        1 atttccatgg tttgccttac aaaatactcc tttaaatatt attcaagctc cttggcgttt
       61 tatgatattt tcaacattgt tcatatcttt ttcagtatct ctatttattt ctccacagtt
      121 ggaaaaattt tcttatacaa ggagaagaaa ttttatacta ttattaatat tagctatttc
      181 aattttaaat tattcatcag tagttaacat ggtgaaagaa atgaattttc aaggaactta
      241 ttctaatgaa caaatggaaa ataaacttga agaatttgag aattttgatt atgtacctat
      301 aggctataaa aaagatcaat tgcttttgaa aaaccatcaa gttacagaag atggtcaagt
      361 tatttatagt agcttttctc caaataaaga tgaaattaca tttacgttta aatcttcaaa
      421 aggcaaaaca atagttttac ctgtattccg gtataaagga ttaacggtta ctgttaattc
      481 aaaaaatgct acagtactaa aacaaggagg tccttttgtt acagtaaaaa gtgaaagtgg
      541 aataaataaa gtaacaatca ggtattcata cacatttttt tctaaaatct cattatttat
      601 ttctataatc acgctaattg ttcttctttt ttattgcata aaattaaaaa taagaacaag
      661 ccatgaatga tttgctagca catattttag caatctaaag atactatttt agatttaata
      721 ggccctttgt caaataatgt tggtaaagag aaatcttaga ataatttgga cagaacagaa
      781 agaagaacac ctctttctgt tctgtttatt tggaattaaa gtctgtagca aaaattgatt
      841 tttagatttg tcccacactc tttcctacgt ttagtcgagt acttttttta agcaaatgct
      901 attcaaagaa gtagtatttg ctatcttttc atcatatcag ctatttgcca acttagtagt
      961 tccttccatc aggtcataga tttggtttct tagatcagca gtaggcagtt tgatagtttt
     1021 gtcagctttt atattgcttt ccatgatgat agcatcctgt gaatctttgc taaatatagc
     1081 agatgtatca aaagagtcga tatttccttg cgacaggata taattatctc cgctatatac
     1141 cccaccacta tatggatgtc cattcgcatt ttcacgccat aatgagaaga gtaagtcttt
     1201 ggaaactagt tttccagata aaacttgtgt aaacagcgta tttaaatcat agacggacat
     1261 actgatatta ccagtcccta attcgttatg gatctctgtt tcagattctg tcagtggttt
     1321 aaagtaatca tcctctttag acatttgata ggatagtgca tgattggatg aatttttgac
     1381 ttggtcataa aattttgtat gctgtaatcc taaaggttcg atgatatcat cggtcaataa
     1441 tttttcatac ggttgatttg ttaatttgat caaaattcca gtcaatatgg taaagttcaa
     1501 tgaatcataa tgagaggtct gataatctgt aaattttaag tgctgaatcg caaattgaac
     1561 gacttcttca tcagtcatcg gtatggttgg agaagcagtt cggtgcaagc ctgagcgcat
     1621 atacagcaag tcacggatag taatttgctg gctgccagca atttgtggat agaattgagc
     1681 cagcttagta tctaaagaaa ggtgcccacg ctcgacttgt tttaaaatca aaagagtagt
     1741 caatgctttt tgaaccgaac caatctgata ataaagagaa ggatcatttg gttgttgcgt
     1801 gagtttattc gcatagccat agcctttttc catgatgatg cgattatttt tctttacgta
     1861 aattgagcca atataatcat tttttcttaa tagttgatcg attttttcaa cgagctgttg
     1921 ttcattggta gtcagtatca actttttttg agctgattga gcatgggcaa ctatctgaga
     1981 gtgttcatta ttgtaaaatt gccatccaag aatagttcct gtagagaacg cactaaggct
     2041 aaacaacacg acaacgatca ttaaaaatga atattttctt tttttagcat gttttccttt
     2101 tttcataata gtttatttag tctcctttct tttttacaga aagaataaag ctaaaatagc
     2161 ttgtatcttg ataacattta ttttaatctt atagtaatgg atttactact tacatttttg
     2221 atagaatatt taaataaaat cattcacgcc aattcaaata tcatgtataa tgaataaagt
     2281 tacgaacttg atagtaagga ggagttgttt tgtcaggaaa caaacaacac ccaagtaaag
     2341 ataaaaaaga agccagtaag gcagctacag cagatataat cgacaagcgg agaaatctgc
     2401 gtaaaaaaga agataagatt gtgaggaaga tcattttagt gattgctctt acattattga
     2461 ttattggcgg atttcttgga tttaccgtgt atcgttatgt agatagcggg ttgaagcctt
     2521 tagataaatc agatgaccaa ttagtccaag tcgagatccc gagtggctct tctaacaagc
     2581 agattggtga gattttggag aaggacaata ttattaaaag cgggattgtt ttcaattatt
     2641 atacaaaatt taaaaattta actggctttc aagctggcta ttatcagtta gcgcctaata
     2701 tgactcttga cgagattggc aaacagttgc aagaaggcgg tacttctgag ccaacaaaag
     2761 ttgcagatgg gaaaattgcc attccagaag ggtacgatat cgatcagatt gctgaacggg
     2821 tagcgaaggt aactggtaaa gacaagaaag aatttttaga tttagtgaat gatgagactt
     2881 tctttaacga gttgcaccaa aaattcccag aactgttgga aagtgcatct aaagctgaga
     2941 acacaaaata ccgtttggaa ggttacttat tcccagccac atatgattat tacacgagta
     3001 cttcgcttaa ggatctagtg attgaaatgg tgaacaagac gaatacggtc atgcaaaact
     3061 attattcagc tatcaagcag aaaaatttga ctgtacaaga agttttaact ctagcttctc
     3121 tagttgaaaa agaaggcgta aaagaaaatg atcgtaaaaa cattgcacaa gtcttcttta
     3181 accgaatcaa agcaaatatg ccacttcagt cagatatctc tgttttatac gcattaggtg
     3241 aacacaaaga attagtgacg tatgaagata cagctgtaga ttcaccatat aatttgtata
     3301 ccaataccgg ctatggacct ggaccttttg acaatccaag cgaagaggca atcaaggcag
     3361 tattagaacc agcagaaaat gactattatt atttcgtagc agatacgagc actggaaatg
     3421 tttatttcgc taaaacttat gaagaacata tggaattagt ggaaaaatat gtaaataatt
     3481 cttagaaaaa caggctctag acattgatca tttttagaaa aacatttaca tttgaagaaa
     3541 agcgtggtaa tcttttttgg actatattcc tcaaaagatt accttttgcg gtattttatc
     3601 gaaaaaagga gacgttacac atggtagaga aagtttatcc aatgactcta gaaggtaaag
     3661 aaaaattaga aaaagaatta gaagaactaa aaacagtcaa acgcggagaa atcattgaac
     3721 gaatcaaaat tgcccgcggt ttcggagatc tttccgaaaa ctctgaatat gaatcagcga
     3781 aagatgaaca agcatttgtt gaaggaagaa tcacaacaat tgaaaatatg attcgttttg
     3841 ctcaaatcat cgataacgac ggtgtagatg cagatgaggt atcaattggt aaaacagtta
     3901 cattcatcga actaccagat ggagaagaag aagagtacac gattgtcgga agcgctgaag
     3961 cagatccgtt ttctggcaaa atttccaatg attctcctat tgcgcaagca ttgattggca
     4021 aacgtaaaaa tgatgaagta actattgcta ctccaggtgg cgatatgtta gttaaaatcg
     4081 tcaaagtaga acaagcgtag tctgactgaa agatctagag agttcatctt actgatgata
     4141 cactctagat ctttttctct ttgtgtattt tagatttcaa caaacgatct tacatctaaa
     4201 gtcctatagc caacagttaa aaaaataggt atgatgaaat gagaatgtgt caggagggag
     4261 aaagagcgat gaatagttct tggcgttttt ttgtggtagt tgaagcacta ctgctgttat
     4321 ttgctgtttg gcaaatcgta aacaatactg gacttttgct tttagtgata ttcggtattt
     4381 tcaatatata tctagctatg cgcaaacatc ctcgtactaa atttcaaaat tttcaattga
     4441 tattaggaag tttggtcatt ttttttagct tagtcaacag tcctgcttta tggatgatgg
     4501 ctgtatttgc cgttttattt attggtctga aaggtgtaga gatttcaggg atagatttaa
     4561 ccaaaaatgc tttttggcga aagaaacaga tcatgatgat ccaaacagag caattaaaaa
     4621 cacataataa tgagcggaaa agacagcaat tattcggtaa ccaacgtatt ggaaacgacg
     4681 tctatgaatg ggacgatatc aacatcgccg tcatttctgg tgatactatc atagatttgg
     4741 gcaatacgct tttgccaaaa gatgataata ttgtgattgt aagaaaaggg ataggcagaa
     4801 cacggatctt agtacctttg ggcgtagcca tccgattaga gcatgcgaca ttagtaggaa
     4861 atgtcttgtt tgaagaagag caattttctt tgaaaaatga acaaatcaaa atctacagca
     4921 gtgattatga tgaaaaccct cgtagattga agattattac taataccttg attggagatg
     4981 tcgaggtgat acgaatatga tgggaaaaat atccagagcg atgctagctg tttattcggg
     5041 gattgcggct ttccttatta ttctattttc acttttcact tatttttatg ccagcaatca
     5101 aagtcattgg tggggagaat tactgcgtgc acgtttatta tatgttccgc ttatcttcca
     5161 tttgttagcc atttccttag gtgtaggatt aattgtcttt ctattattat cacttattca
     5221 aaagacaaaa tacgggaaaa tcgaagaaaa gctgcgtgca ctttcttctg gcaattatga
     5281 atccaaattg ttgcttcttc cgatcccaag tgcatcggac gatttataca tcaaagatat
     5341 tgataaggaa atcactaaga taaaagaaaa aatgattgaa gtatctagtg aattacaaat
     5401 tgtaacgagc cgtccccaat atgtagatgg acaaaccaaa gaagaaattt tagaattaga
     5461 aagacatcga ttagcccgtg agctgcatga ttcagtttcg caacaattgt ttgcagcaat
     5521 gatgatgatg tcggcattga cggagcaagc agaaaaaagc gagacaccag aaatgtttcg
     5581 caagcagttg aaaatggtag cagagatcat caacgcttcc cagtctgaga tgcgtgcgct
     5641 gcttcttcat ctacgtccag tcaatttaga agagaaaagt ttaaagcaag gtatcgagca
     5701 gttattgaag gaattgcaaa ataaaatcca gatttcgctg aaatgggatg tagaagatgt
     5761 aaaactatct agttccattg aggatcactt gttccgaatc gttcaagaat tgttatcaaa
     5821 tacattaaga catgctaaag ccaatgaatt agaggtatat ttgcacaaaa tagacaataa
     5881 tcttttgtta cgtattattg acgacggaac agggtttaat atgaatgaaa cgaaaacggg
     5941 aagttatgga ttgaacaata tcaaagaacg agtagctggt attggtggaa cagtaaaaat
     6001 cattagtttt aaaggtcaag gtacaagtgt agaaatcaag gtccctttga tgaaggaggc
     6061 atagataagg atgataaaag ttttattagt agatgaccat gaaatggtgc gcttaggcgt
     6121 ctcttcttat ttatctattc aagaagacat tgaagtaatt ggagaagctg aaaatggacg
     6181 acaaggctat gagaaggcga tgacacttcg accggacgtc attttaatgg acttagtcat
     6241 ggaagaaatg gatggtatcg aatcaacaaa agcaatctta aaagattggc cagaagctaa
     6301 aatcattatc gtaaccagtt ttattgatga cgaaaaagtt tatcctgcca ttgaagcagg
     6361 tgcagcaggg tatttgttga agacctctac cgcacatgaa attgctgatg ctataagggc
     6421 aacgcaacgt ggcgagcgag tattagaacc agaagtgaca acaaaaatga tggaaaaaat
     6481 gagtcggcga aatgagcctg tattacatga agaactaacg aatcgggaaa atgaaatttt
     6541 aatgctgatt tctgaaggaa aaagtaatca ggaaatagcg gacgaattgt ttattacact
     6601 gaaaacagta aaaacacatg tttccaatat attggctaag ctagaagtgg aagaccgtac
     6661 acaagccgct atctatgcat tcaaacatgg tttggtgaaa taaaaacgat gataaagagg
     6721 ataatcaaaa gctgagaaaa taactattat ttttcttggc ttttttacaa taatgatgct
     6781 tgcttttgtt tttgtaaagg ctttgttata ctgagaaaag aagatactaa aggaggaaaa
     6841 cggtgaaaca aaattttgca attgtaggtc ttggacgatt tggcggaagt atctgccgaa
     6901 cactgatcga agctggtcaa gaagtactgg ctattgatag taatgaagat cgggtcaatg
     6961 aatatatgaa tatagccact catgctgttg tggcaaatgc acaggatgaa atgacacttc
     7021 gatcattagg cataagaaat ttcgatcatg taattgtcgc tattggagaa gatattcaag
     7081 ccagtatctt agttactctg atggtaaagg aaatgggcgt accaaatatt ttagcaaaag
     7141 cccaaaatga gtatcatgcc agagtacttg aaaagatcgg agctgatcgc gtcgtccatc
     7201 ccgagcgaga catgggaata aggattgcgc ataatttagt gtcaaagaat attttagatt
     7261 acctagagtt gtcagataaa tattctttag cagaaatccg agtctccaat cctaaatttt
     7321 tcaacaaaac attagctgaa ttaaatttca ggcaacgttt tgacttaaca gtagtcgcca
     7381 tacgaagagt agatcaaacg gttatcgctt ctcctgcagc agatgaatat gttcgtgaaa
     7441 atgataatct actggttatt ggagagacag gcgatgtgga tattctagac gataagatga
     7501 atcaatagaa agaaaagaga aagtaggttg ttgggatgaa attaacggta acgccaaaag
     7561 cacaaaagtg gtttgactca gaaattgatc tgccagaaac ttacggaata cgtttttttg
     7621 gaaaagtcta cggcaaaaca gaggttcatg atggtttttc gattggaatg tcagttgaac
     7681 agccagaaca tcctgtaaag aaagtggaaa tggatgggac actttttttt atcgaggaag
     7741 cagatgaatg gttctttaaa ggatatgatt tacttgttga ttatgatccc aacctggagg
     7801 agccaaccta tcactttaaa aaacaataaa aaaagagagg gtgacacaat ttttgtcacc
     7861 ttctcttttt tcttttgaaa atattgtttt cagctattaa tatcctgcag acaattttac
     7921 ttgattggtt tgaagctcat gggtggcaaa gtagcttttc agattggtta aaaaaatagc
     7981 cattaatttc ttttgaaaat caggtgtcaa acctgcaatg tgggaagtaa tcagtacatt
     8041 gtccattttc caaagaggat gatcttgtgg aagaggctct tcctcaaaaa cgtctaatgc
     8101 tgcaaaggaa agttgctggt tatttaaagc ctgcaccaga tcttgtgtct taacagaagc
     8161 accacggcca acattgatga atacagattt tggatcaaag tgttcaaaca tatgctgatc
     8221 aaagagccca gttgtttcct ccgttagagg taagatattg actacgaaat ctacttcagg
     8281 aagaacttcc tttagttgat ttaaagaaaa agtcttttcc attccttctg ctggatgacc
     8341 ggatgtattg attccatagc agttcatgtc taatgctttg catttttttg caatcatttt
     8401 tccaatatgg ccagttccaa tgattagtat ttttttccct tggatagatt ggtaatggac
     8461 cgatgaacct aaccatttca tgtcactctg tgccttttgt gcttgaaaaa gaccacgaga
     8521 atacccaagg atgattccta agacatgctc cgtaatcgat tcactgtgga tgccactacc
     8581 atttgaaagt aaaatatgtt tctttgaaaa agtctcaagg ggtagctgat caacaccagc
     8641 tgagatagat tggacccatt tcaaggtaga agaggctaat agtttttctt ggtagtctga
     8701 gttccagccc aaactgatct ctacattttg tagatcctct ggttgtaatt ctgttttaaa
     8761 gatataacca ggcgctatag tatggatttc ttcgataaat tcatcacgaa atggacgatc
     8821 tgcataaatg attctttctg acatagttaa tttcccgcct ttctttactc taagtgtagc
     8881 gcatacaaga agaactttga agcgccttgc ttgttttttg agaaagaaat atttttcaaa
     8941 taaaaaaggg gctgtgacaa aaaaatgtca caacctctaa aagggtctat tgaagtagaa
     9001 ataacgagtt gtctttattt cttaattgat cgatattttt gcccatcctt tgacagcttg
     9061 agcaaaaata aagattcctg caaaagcaat gaaggcgcta gtgtcaaaag aaggaagaaa
     9121 gaccatcaag attgttgcaa ttatagtagg caatgaaatt gaatagatca atgtttttaa
     9181 acaatcaaga aaagtagctt ttgttagtcg aaagcgtgca tagagaaaag cagcaatgtt
     9241 ggtaaatagc aaagtgatca gcagattcag aaaactagga taaatagaaa atataaatgt
     9301 caggactttt atccaaagtg gtatagatgc ttcacttaaa aaagaacgga gctgcttacc
     9361 tgttaagttt tttaaagatt cattggtgta gggaagttct aattggttat tgttcaacaa
     9421 agcagaagtc gtgcctgtat taggaagagc tatgataagt ttgtttttta acatcccgac
     9481 actaacaaaa ttccccatca aatcagtaga gatgtcctgt tctgttcgtt ttccctctgg
     9541 atcaaatgtg aagataatgg agtttgtttg gtaaatgaat ccttcttttt cttcggtttg
     9601 aagctgcccg ttttggattg taaaatcagg aatgcgagta gcaatctttt gcccatccaa
     9661 ttgaatgttg tttaagactt gaaaaacttg aaaagagatt ggcaaagcca taattatact
     9721 caaaactaaa agatagacaa tggctttcca aaatgggaca tttctcgcct cctttaggtc
     9781 tttgaatcgt ataaaggaac tgataattaa ttgttttgtt gtcataagta ctcctcatct
     9841 agtagtgtca tcacttgcaa ttctagcgaa gtatcaagtg aaatacaagt gctttaagga
     9901 aacagctgaa tgattggtga aatatcaaaa aaatgttatt cttgtctaaa gactatccaa
     9961 tataaataaa atggaggaag gaatagtgcc tacacagcca cagagatcat caaaaaaact
    10021 atttggagta gtggttttga atgcggtgat cactttatca gaatttattg gagggattat
    10081 gtcagggagt ttgtctcttg tttcagatgc tttccataac cttagtgata cattggcaat
    10141 tattttttct tacggggcac aaaaaatggc ccgaaaagaa gcaaataaaa aacgtacgta
    10201 tggctaccag cgtttagaaa tattgagtgc tttcatcaac tcattcatat tgatcatatt
    10261 atccttgttt ttaaccgttg aagcattcaa gcggttcact tcaccagaaa agataaatag
    10321 tcatttgatg ctaacagtag cagttattgg attgctcgcg aatttgttct cagtattatt
    10381 attacgacaa gaagcagatg aaagtttgaa tataaaatct agttatttgc atttgttaag
    10441 tgatacactg tcttcggtaa gtgtgattat cggagcaatt ttgatccgtt ttttcgatat
    10501 ctactggatc gatccagtta ttacactgat catttcgata tatatattga tagaagcgat
    10561 aatggtgatc aaaaaagctg ccgctatttt gatacaaagt gcacctgcta tcgactatga
    10621 aaaaatggaa caagaaattg aagcaatcga tggggtaaag gatgttcatc atgtccatat
    10681 ttggcagtat agcgaaaaga taattatttt cgacggacat attgattttg aagatcagct
    10741 actaagcgag attgaaaaga tatatcctcg tataatttct ttattaaaat taaaatatgg
    10801 tattacacat gtgacgatcc aagcagagac aaaggcaaag gatcaaaaaa aattaatttt
    10861 tttaaataaa gatgaaagtt attgaaatat agagtatgat aaataagaat ttttaataag
    10921 gtaggttggc tcatgaagca gtatcgacag ttaaaaggat atttagaagc aaaaggatat
    10981 tgggagatac tagtctatct cttttttggt ggtttggcta cgattgtaaa ttttgttagt
    11041 tttgctctcg cacgccaata ttttgattta agtatggcct tatccaattc tatttcgtgg
    11101 ttttgttctg tattgtttgc ttttgtaacg aacaaacttt gggtattcca ttcaaaatca
    11161 ccgaatttta cacatgcact tattgaatgc ggtaagttct tcttctaccg tatcctttct
    11221 tacggattgg acatgggggc aatggtattg ttgatcaatg ttatgaacag caatgagtat
    11281 gtggcgaaaa tcatcactca aattattgtt atccttgcga actatatttt cagcaagttg
    11341 tttattttta aagaaactga ggtttttgaa gaagaagtag gtcaaagtga aaaagacgac
    11401 taggcataac aattgaagaa tttccttctc tttgttactc tttgtaatgt agacattatt
    11461 aagaaaacat tgaggaggaa tttttttatg acttacacat taccagatct accttatgca
    11521 tatgacgcat tggaacctta tattgacgaa gaaacgatgc atctgcatca tgataagcat
    11581 cacaatactt atgtgacgaa tttaaattca gcaattgaga aatacccaga attaggcgaa
    11641 aaaacaatag aagaattatt atctgatatg gacgctattc caacagatat caagacagct
    11701 gtacgtaaca atggtggcgg acatgctaac cattcatttt tctgggaaat catggcacca
    11761 aatgctggtg gcgaacctac aggagaaata aaagaagcga ttaatgaagc ttttggtgat
    11821 ttttcttctt ttaaagaaga attcaaaaaa gcagccgctg gacgatttgg ttctggatgg
    11881 gcttggcttg taatggaaaa tggaaaatta gctattacct ctactgcaaa tcaagattct
    11941 ccattgatgg aaggaaagac accaattcta ggtttggacg tttgggaaca tgcttattat
    12001 ttgaaatata aaaatgtccg accagattat attgcagcat tctggaatgt tattaattgg
    12061 gatgaagtaa acaaacgttt cgaagcagca aaataaccat ttcctccgtc atatcagtct
    12121 taaaaataat ttggaaaata aaagagcgaa agcttgctag cgttttctaa attgggtata
    12181 atgttcttgc aaggttactt gcaagaactt ttttggagga tatacattta tggaacacgg
    12241 aacggttaaa tggttcaaca atgaaaaagg atttgggttt attacagtag acggcggaga
    12301 tgacgtattt gttcatttct cagcaattca aggagacggc ttcaagagcc tagaagaagg
    12361 tcaagaagta gaattcacga ttgttgaagg tgcacgcgga cctcaagctg ctgaagtgac
    12421 taaattataa taatttgttg
//
LOCUS       DAZULY010000075        12407 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000075 DAZULY010000000
VERSION     DAZULY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.076"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            251..526
                     /locus_tag="ACG81A_001321"
     CDS             251..526
                     /locus_tag="ACG81A_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014692.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            539..2611
                     /locus_tag="ACG81A_001322"
     CDS             539..2611
                     /locus_tag="ACG81A_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS5014693.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2779..4662
                     /locus_tag="ACG81A_001323"
     CDS             2779..4662
                     /locus_tag="ACG81A_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5014694.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            4674..5621
                     /locus_tag="ACG81A_001324"
     CDS             4674..5621
                     /locus_tag="ACG81A_001324"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFS5014695.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5641..6168
                     /locus_tag="ACG81A_001325"
     CDS             5641..6168
                     /locus_tag="ACG81A_001325"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS5014696.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6231..6884)
                     /locus_tag="ACG81A_001326"
     CDS             complement(6231..6884)
                     /locus_tag="ACG81A_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFS5014697.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7016..7858
                     /locus_tag="ACG81A_001327"
     CDS             7016..7858
                     /locus_tag="ACG81A_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5014698.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8016..8912
                     /locus_tag="ACG81A_001328"
     CDS             8016..8912
                     /locus_tag="ACG81A_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS5014699.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8915..9628
                     /locus_tag="ACG81A_001329"
     CDS             8915..9628
                     /locus_tag="ACG81A_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFS5014700.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9648..10166
                     /gene="msrA"
                     /locus_tag="ACG81A_001330"
     CDS             9648..10166
                     /gene="msrA"
                     /locus_tag="ACG81A_001330"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS5014701.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10163..10390
                     /locus_tag="ACG81A_001331"
     CDS             10163..10390
                     /locus_tag="ACG81A_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFS5014702.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10542..11093
                     /gene="lepB"
                     /locus_tag="ACG81A_001332"
     CDS             10542..11093
                     /gene="lepB"
                     /locus_tag="ACG81A_001332"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS5014703.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11176..11718)
                     /locus_tag="ACG81A_001333"
     CDS             complement(11176..11718)
                     /locus_tag="ACG81A_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS5014704.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11955..12353)
                     /locus_tag="ACG81A_001334"
     CDS             complement(11955..12353)
                     /locus_tag="ACG81A_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5014705.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT         4311 a         2121 c         2486 g         3489 t
ORIGIN      
        1 tcgcagcccc atttttttgt tgtgataaaa gaacaagaaa ggcaggaatg ggaaagtaaa
       61 catccttttg tcagaaactt ttatctttct taagttttac ttgatatctg atactatttg
      121 gaatagaatg aggcgttgaa aaatttatgg aaatttctaa ctagcaataa acgatgaaac
      181 aatgaaatat ttttcatcca aaaagaactt tgctataatg atggaagttt gtgtatagga
      241 gatctggaaa atgagagaga gttttgaaca acaaaaaaaa ctgttgcatg atcgctatgg
      301 cgccctttcg atggatgacc gccgtcaaat actttgtaag ttgcgtaaaa gaaatatatt
      361 gatgtatcgt cagttggaac gtctgaaaca tgatctgctt cgtttggagt caaaacgtgt
      421 acagtgtgaa ttagagggga accaaacaca ggttgaagta gtcgaaacca aaattttaaa
      481 gaaaaaagaa caatttttaa aaatgcttac acaaaataaa aaataaaggg gatggacgat
      541 ggaatttgct gagttagtta ttgtatttgc agtaacgatc accttatcga acatagctag
      601 ccgcattttg ccaatgattc cagctccact gatccaaatt ttccttggag tggtattagg
      661 tctgacagag tggggacaat caatcgattt tgaacctgaa ttgttcttag ttatgattat
      721 tgcaccattg ttatttagag aaggagaaaa agcagatatt tcttcgattc tcaaaaattt
      781 cgggacaatt ttgttcttgg catttggagg agtcattttg acccttgtcg gggtaggtgc
      841 gacgttatca ttcctgttgc caagtgtccc acttgctgcc tgctttgctt ttggagcagc
      901 gttaggtccg acagatgcgg tagcagtcag ttctctttct ggacgagtga atatccctaa
      961 aaaggcgatg catattttgg aaggagaagg tcttttgaat gatgcatctg gtgttactgc
     1021 cttccagttt gctcttggtg cgctgatcac cggatctttt tcagcagtaa acgcggggat
     1081 gagtctagta gtttctagta ttgggggagc tttgatcggc tttttgcttg tctggttcaa
     1141 acaaaaaata attcatttga tcgaaaaagc atcagcacaa gatgtcacag ggtatctgct
     1201 gatcgaactt ttgcttcctt ttctagccta tgtattggca gaggcatttg atgtatctgg
     1261 aatcattgct gcagtggcag caggtattct ccaagcttct ggttttagga aaatctctgt
     1321 gtttgatgca gaattgtcga gcttgtccca cagtacatgg acaactattg cttttacact
     1381 gaatgcatta gtatttatat ttttaggaat cgaattgacg caagtgttct cgccagtatg
     1441 gggagacggt ttatatccaa acggtctttt attagcgatt atcgtattaa tctctgtcat
     1501 gctttttgtg atcagattca tttctatcag tcttttttat gtattcaaag atggcagtaa
     1561 aaagttcaaa aaacagttga acgagatctt gatcttaaca tttggcggtg taaagggaac
     1621 agtcagtttg gctacgattt ttattttgcc attttcaatc aataatatga tgttttacca
     1681 gcggtctttg cttttgtttc tgacggcggg tgtcatctta gtgactttgg tcatagggat
     1741 cattgtcttg cctatgctga cggaaacaga ggaagcgaaa agcacggatt tgaatgcttt
     1801 gatgatttta gaagaggtcg ttgaagtttt gcgaaaagaa ataaaggaaa ttgatcgtaa
     1861 tacaaaagaa tttttagcca ctgaagcagt gatagaaaat tatcaagaac ggatacgtga
     1921 tctgtacttg gaagatttaa ccgatgatga aaagcaagaa gtacaagaaa tacaagcact
     1981 gattttgtcg atcgaacggg atggattgga tgaaagttat cgttcgggca agctttcttc
     2041 taatggttat cgtttttact cccgttttct ttctcgattc gaacattcta tcacaagcca
     2101 gattttgtct tttatcggct tttggttcat tgttgtccgt aggttaatgc gaatcgttct
     2161 ccatccgaaa atgttttggc aaagaagata tgctgatcgt cagacattta ttagtgatga
     2221 agatatccaa gaaatcagag agacatatca aaaaaatact cagttgatta ttgaaagttt
     2281 agataacttg actgatattt atgatgaaac gatggttcaa ttttttatcc agcaacgcaa
     2341 acttgaagga ataaaaatga actcggggaa tttgatttct tcgtggatga tccagcaaga
     2401 tagtctgttt acgaaaaaaa tgctaagagg ttattatctt gaaagaaaaa tcattgatga
     2461 atacgaagtt gctgaaaaaa tcacgacgtt ttctgcgaac agctatcgca ggaatatcaa
     2521 tttgttagaa tcttatacga tgaacaaacc atctgataac ttttctttcc gatttgcgtt
     2581 tcgagcaaag tcaaaaaaac gagtccaata aaataaaaag gtaaaaattt tcaagtttta
     2641 taaaaaacaa aacatatcag aacaggatcg aatcggtcct gttctttttt tgagaacaca
     2701 aaaagaacaa ataccaaaaa aattttcctg aagttcacta tttttatgat acaatatcca
     2761 agataaggag ggaacatgtt gaaagaatta aaagtaactg acctcaaaaa aacttatggt
     2821 gaaaaagatt tgtttgacca gatttctttt ttggtccacg aaaaagaccg tatcgggttg
     2881 attgggacga acgggacggg taaaacaagt ctcttaaata ttattgccgg tatcgatagc
     2941 agcgacggtg atcggcaaac agtgttttat ccaactgatt accgtattgg ttatctttct
     3001 caagcagctg aattttccga tgaattaact gttctccaag cagtctttca aggaaacagc
     3061 cccttgatcc aaactgttcg agcatatgag gaagcattga tcgaactagg ggaaaacgga
     3121 gaagaccctg atgtgcaaaa acgttatgct aaagcagaag aacaaatgaa taaagaagat
     3181 gcttggacta cagataccaa cgcaaagatt attttgcaaa aattaggaat cagtgaatta
     3241 gataaaaaaa tcagtacttt atcaggtggt cagaaaaaac gagttagtct ggcgcaagtg
     3301 ttgattgatg aacccgattt actattacta gacgaaccaa caaaccattt agattatgaa
     3361 gcgatcgagt ggttggaaaa ctatttgaaa caataccgtg gcgcactatt gatggttacc
     3421 cacgatcgtt attttctaga tagagtagca aaccgaatct tcgaactttc atttggaaaa
     3481 ttatacgagt ataaaggaaa ttatgaagct tacgtcttag aaaaagcaga acgtgatcgt
     3541 gtagaagtag aacaagaaga aaagcgcaag cggctttata aacaggaatt agcttggatg
     3601 agggctggcg tacaggcacg tggcaccaag cagcaagcac ggatcaaccg tttcgaagat
     3661 ctaaaggaga acttgtacca agtaaaccaa gaagatgatt tagagctgaa cctagcgaca
     3721 caacgtttag ggaaaaaagt actggaaatc aaagatggca gttaccgtat aaatgaccaa
     3781 acactgctag aacatttaga tttattgatt caatctcggg aaagactagg tattactggt
     3841 aaaaatggtg cgggaaaatc gacattgctt aatattctag ctggcagaat tcccctggat
     3901 agcggtacaa tgtctatcgg tgaaacggtt catcttgcgt attatacaca agaaaatgaa
     3961 gaaatggcac cagataaacg gatgattgct tacttacaag aagctgctga agaagcaaaa
     4021 acagcagatg gcagtcaaat cggagtagca gaattacttg aacgattttt atttccaaga
     4081 tttatgcatg ggacactgat aggcaaactt tcaggcggag aaaaacgccg gttatttttg
     4141 ttgaaactgc tgattcagca acctaatgtt ttattattgg atgaaccgac caatgatttg
     4201 gacatagcaa cattgacgat tttggaagat tattttagaa gttttccagg tgctgtgatc
     4261 actgtttccc atgaccgtta tttcttggac aaggtagcag ataagttatt agtattccaa
     4321 ggaaacggaa aacaagaact ttattacgga aacatgtcca gttatttact gaagcaaaaa
     4381 gagacccagc agcctgctga aaaagcgaaa ccaaaaatac agagcaagga acctgctgaa
     4441 aagaagaaat tatcttacat ggaacaaaaa gaatgggaaa cgattgaaga tgagattgca
     4501 gaacttgaag aaaaaatctc attgctccaa gaagagatga atcatcaagg agataatttc
     4561 actcgattac aagaattgca aaacgacgta tctgaaacag aagcacaact tgaagaaaaa
     4621 atggcacggt gggaatactt gagtgaatgg gtagaagatt aggaggaaaa aggatggaac
     4681 aagcgtatct tgacttagga agaaaattgt tggaagaagg gcatcgtaaa gaagacagaa
     4741 caggcacagg cacattaagc ttattcggat accaaatgcg gtttgatctg caaaaagggt
     4801 ttcctttgtt aacaacgaaa cgagtacctt ttggattgat caaaagcgaa ttgctttggt
     4861 ttttaaaagg agatacgaat atccgctatc tattacaaaa taaaaaccat atttgggatg
     4921 aatgggcatt tgaacgctac atcaaaagtc cagactataa tggaccagat atgactgatt
     4981 ttggccatag atgtttgaca gatcctgaat tcaatgaaga atataaaaag cagagcaagc
     5041 ttttttgcga gaagatccta accgatgatt cctttgctga aacatacggc gatcttggac
     5101 acatctacgg ctatcaatgg cgtcactggg aaacaaaaga cggtggcttt atcgatcaaa
     5161 tcaaacaagt gatcgaagca atcaaaaaga ctcctgattc tagaagattg atcgtttctg
     5221 catggaatcc ggaagatgtt cctagcatgg ctttgcctcc ttgtcatacg atgtttcaat
     5281 tttatgttca agagggacgg ctaagctgcc aattgtatca acgcagtggg gatgtgtttc
     5341 taggcgttcc gttcaacatt gcaagttatg cgttgctgac tcacctaatt gcacatgaaa
     5401 caggtttagc ggttggcgaa tttgttcaca cattaggaga tgctcatctt tatatcaatc
     5461 atatcgaaca aatgaaagaa caattgagcc gagaaatacg cgcatttccg actttagttt
     5521 tgaatcagga aaagcagtca gtatttgatt ttgagatgga agatatccaa ttagaaggat
     5581 atgacccaca tccggcgatc aaagctccta tcgctgttta gaatcagaaa ggatgagaaa
     5641 atgttagctg caatttgggc acaggatgaa aacggattga ttggaaaaga agaccgtctt
     5701 ccttggcgtt tgccaaatga tctgaaattc ttcaaacaaa tgacagaagc aaatacacta
     5761 gtgatgggac gaaaaacatt cgaagggatg ggtagccgtc cgttgccgaa tcgtcagacg
     5821 attgttttga cgagagattc ttcctataaa gcagaaggcg tgcatgtcat gcatagtgtg
     5881 gaagacgtgc ttgctctgga aaaagaaaca gacggtatct ttttcatcgc tggagggtca
     5941 gctgtttatc aagaatttct gccattttgt acgatattat accgtacagt catccatcat
     6001 gtattcgatg gagatgctta ttttccacct gttgactggt ctgattggtc actgatcaat
     6061 actagtcaag gagaaatgga tgaaaaaaat cgttatccgc atcaatttga aacgtatcag
     6121 cgtaatgaga atcctgtaaa aagcgggaca ggcgaattga cgatataaaa aagctatgga
     6181 tagactagct gttatttaga gctgtcccat ccatagcttt atttttttta ttaagtaaag
     6241 aataaaacag agaagtacat aaaacctgca cctaacataa caaacaaatg ccaaacaaca
     6301 tgcatatagc ggatagaatt caagctataa aagaatgcgc ctatcgtgta agaaagcccg
     6361 cctgctacca tcaaaataac accagtcatt ccaagagagt gataaagagg aacagcagcc
     6421 acgacacaga gccaccccat aaaaatatag atgatcgtag atacttttga tactctctcc
     6481 cgtttatgca acgtcaacgc tttatatata atgccaagaa tcgcaaacaa ccagattaat
     6541 acaaataaag tccaacctag ccatcctcgg atactcagca aacaaaaagg agtataactt
     6601 cctgcaatca aaagaaaaat cgaactatga tcgaatactt ggaacacctt ttttgctttt
     6661 gtaaaaatca atgaatgaaa caaagtagac gttaaaaata aaagaatcat catcgatcca
     6721 tagatagcat aagagacaat atggagggag gaatgcaagt gggcaccttt tactaaaaga
     6781 accactagtc ccgcgatact caatccagtc ccaattccat gggtgactgc attaaacact
     6841 tcatttaata tttcatacgt tcgattaaat ttcgtttttt ccatgaaaac ggctccttta
     6901 ctaggtgtaa ttaatgaaaa atcattattt gtctgttata ctaaaaatag aaattgtctt
     6961 acatgtagtt taacatgaat aaggcatctt gttaaagaaa gagagagaga aaataatgac
     7021 aaatgtaaaa atcgtaacag attcttcatg tacgatggaa aaaaatgtac gcgatgcttt
     7081 aggcattcgt gtcatgccgc tatccatcat gatcgacgga acagtatatc ccgatgatga
     7141 ccaactagac ggcgaaagat ttatggaaat gatggcgaaa gcaaaaaatc tgcccaaaac
     7201 aagccaacca ccaattggag aattcatctc gatgtatgat gagctaggag aagacggcag
     7261 tgaagtgatt tcgatccata tgacaaaagg cttgagtgga acagtagaag cagcaagaca
     7321 agccagtaat ctaacaaaaa cgaaagtaac ggttatcgac agtgatacca ctgaccaagg
     7381 attatctttt caggtaattc gagcagctga attagcaaaa gaaggtaaaa cagtagagaa
     7441 aatccttgaa gaagtcaaaa aagtacaaga caatacaaaa ttatacatcg gaatatccac
     7501 actagataac ttggtaaaag gtggaagaat cagccgaaca actggtttgc tgtctagtgt
     7561 tttgaacata cgcgtggtga tgaatttcgc ccattctgaa ttaatccctg taactaaggg
     7621 gcgaggaaag aaaacgttcg ataaatggtt taataatttg aaaaatgaac tgaaagaatt
     7681 gccaaatgtg cgacaaatcg gtatttcaca tgcggatgca aaagaattgg cgcaagaatt
     7741 caaagagggt ttgcaagaat tattcccgga catgcatatc cctgtactgc atacgaatcc
     7801 ggttatcgct acgcatactg gtaaagacgc gtttgcaatc atgtattatg tagattagtc
     7861 gaaaagagga taagccctca gcctgaaagg cttgcagctt attctctttt tctttataaa
     7921 attaggaaaa taattgtttt cttttttcat aaagactacc tttttgataa aatttttgat
     7981 aacattagaa ccaatcgaat acaagaggtg aaaaaatgaa attaacgaaa cgacatttga
     8041 tcctcgtttt gttcccatta ttaattgcga tcatcatcta tgcaggttta gctgcttcta
     8101 taccgaaagc tggcccacta atgaaaccag taactactgt gagagaagat ccaaataaaa
     8161 aagaaaaact tcactacata gctatcggag attcgctgac tgaaggagta ggagatcaaa
     8221 ccaagcaagg aggattcgtt ccccttgtcg caaatgatct aaaagaccgt tataatttga
     8281 ccgcggttga aacagaaaac tatggtgtaa atggtgaacg aagtgaccag atcttaaagc
     8341 gagtaaaaac aaaagaagaa attcgaaaca atatcaaaac agctgatttt atcacactga
     8401 ctgttggcgg taatgatttg atgaaagtta tccaaaataa tttattcggg ttaacggtca
     8461 attcctttaa aaagccactg aaaaactatc aggaaaatgt cacagaacta ttggaagaat
     8521 ttcgatccct caataaaacc gcacctattt acattttagg catctacaat ccattttatt
     8581 tgaactttcc tgagatcact gacatgcaga caattgttaa caattggaac aaaggaacag
     8641 aaacggtgac aaaaaaaatg ccgaactgct actttatccc aatcaatgat ttgctttatc
     8701 aaggattgaa tcaggaagta ggtgtcacag aagaaggaac agatcaaaca actagcacga
     8761 cagaaaatct aaatggcatc aaaaataatg tgctctatga agaagatcat ttccatccaa
     8821 ataatttagg ttatcaattg atggcaaatg cagtgagaga tcagctgatc gagacacaaa
     8881 accaatggtt agagaaggag gatgatcaat aaacatgaaa acaagatctg acgtatcttc
     8941 aaacaaaaca aagaaaacaa attactggaa attcgccttc cttatccttc ttggggtagt
     9001 acttggttcg accgtatttt taggcagccg tatttttgct aatcgggaac cagaactccc
     9061 agaaatacca gctcttacgg aaagacaagg agatcctgtc ttaacgatca attcaaataa
     9121 agaaaaagtc aatcaaatca tcagcttttt ccttagtgag tatcaaaaag atagtgatat
     9181 tgaatataaa ttttatttgg aaaatgaagc actactcaat ggtactttcg aagtactagg
     9241 ttttcctatc gatttctatt tatattttga tccgtatgtg atggaaaacg gaaatgtcca
     9301 actaaaagct agaagtcttt ctatcggaac attgagtttg ccaatcaaag atgtgatgaa
     9361 tatgatcaag agaaactata aattaccaga ttggatcgaa gtcaatacag atgatctgac
     9421 tatcatgctt aggttggatc aattccgaat gcaaaacggg atgtatatta aagcagataa
     9481 aatcgactta gtaaatgatg acattcggtt tagtctatat ttgccaaaag atacctctga
     9541 tgcttcaaac aactcaaata ccaacaaaac tagcaccagc agtactgaaa gcaagacaaa
     9601 agaaaataaa aaatcatcgg atgagtagta ttagaggggg aatcataatg gaacgagcaa
     9661 tatttgcagg aggctgcttc tggtgtatga tccagccttt cgacacgctt cctggtatcc
     9721 atacagtgat gtcaggatat actggagggc atgtaccaga tccgacttat gaacaagtaa
     9781 aaagccaaac gactggacat acagaagcag tggagattat ctatgatcca acattgattt
     9841 catatgaaca attagtagaa ctttactggc agcaaactga tccaactgac gctttcggac
     9901 aattcgagga tcgcggtgac aattatcgcc cggtgatctt ttatttgaca gaagaacagc
     9961 ggatcattgc tgaaaaaagt aagaaacagt tagctgaaag tggacgtttc gaacagccaa
    10021 tcgttacaaa aatcgaacca gcccaaccat tttatctagc agaagaagac catcaagagt
    10081 attataaaaa gcatcctgaa aattttaaaa gaaaccatgc acgccgggct gcctttatcg
    10141 aagaaaattg gaggaacgaa gggtgaatcg aagctttttt cattatttaa tgacactgcg
    10201 aggcggtggc acacatgatg ctttatctgt tttcgctaca gaagccggta aagacatcca
    10261 atttcctaag cacagctcta gctacgaaga aatttctaat tatctggaac tgaatgtcga
    10321 ctatttgccg tccatggata tctttgacga agcatgggaa aaatatttag aaaataatca
    10381 tgacatataa gagatagttt tgtactttat gatcaaaaaa gttttttgtc caatagccta
    10441 attgaaagaa ttatttttat ttcagctatc tagtcgcatt tttttgatat ttttcttata
    10501 ataaagaaag aatgaaaaaa agagggatgg attgatagag attgacaaaa aaacaacgct
    10561 atattaatcg cttctggctt gtattcaaat acttacttgt ttctgtattc cttgcgttca
    10621 tgttgcgggg atttttattc atcccggtac cagtggaagg caattcaatg gaaaacgtgt
    10681 tgaagcaagg tgatatggtt gtgatggaaa aattttcgga gatcagacga tttgatattg
    10741 ttgtttttca attagcagat ggcacgattt atatcaaacg agtaatcggt cttccaggag
    10801 aaaatgtcag ctaccaaaat gatcaattga agatcaatgg gaaagtagtt aaggaacctt
    10861 atctaactaa aaacctgaaa tcagatcatg caaatgcatc ctatacgacg gactttacac
    10921 tacaagagct aacgggacaa tccaagcttc cggaagataa ttattttgta cttggagata
    10981 atcgaagagt ttcaaaagat agtcgttcgt ttggcacgat caataaaact gatattctag
    11041 gtaaagcaag atttgtctat tatccacttg atgaaataaa atggatccag tgagaaacaa
    11101 taaagcgtgc agtctgttga gactgcacgc tttattgttt aatccaaaat cagtttacta
    11161 ccttagtacg taaaatcaaa cagctcgttt aaacactttt ctagcctcta aagttttgat
    11221 aaatgaataa acaaccatta tttgaatagg gatttggatc atattcctga tgaatctacc
    11281 tagaaaaatt actttgaaat ctgcaccaaa caaaacagac atcagccaag aattgataaa
    11341 aaggctgact gggatcataa tcaataattg ggcagcgatg acggaaggca ggtttatctt
    11401 tttatgatat aaaaataagc cattaataaa tccaccaagg aaagccgtga taagaaaagc
    11461 agcttgaaac gttcctgaac caaatatcgt gaaatttaaa gtgttcgaca acatttcaag
    11521 caaaccagcc cagatcggtc catatagata acccataagc gccatactta aaaatccgaa
    11581 tgtgatttga aaataagcag gaccaatcgc tatcttgtat aagatgacat caagggctaa
    11641 taaaagagca gaggtcgaca acaatcttgc attcatttcg ggcaatctaa aaacgtcttt
    11701 tgcaatcaaa gtgttcaaaa tattctctcc tttgtcagtt aataataaaa ggataccata
    11761 aaatcaccaa tgtgtcatga cacattggtg atttacatac actggcatct aacaattaaa
    11821 aaaagggtga gaagactgct tttataaact gtaaaaaaga gattgtacga ataaaaaaaa
    11881 tagaggcgga gaatacttat cgtatcatcc gcctttgttt ttttgttata aaaaacgtca
    11941 gattagttat ctgcttacat cttcatccat acgggttcta atgtatttcc gtctagatct
    12001 tggacttcta atgaaaacat ttcttcttca ggcattccca tatctacttc gtggaaagta
    12061 ccaccatttt cttttgcaat ttgagcaaat ttttttactt catccgcgtt ttcaacacta
    12121 aacgaaatca aagcaccact tgttttttta ttatcagcta tttctttatc ttttataaat
    12181 tttttataaa aatcatgaga gagaagcatt atccaaaaag tgtcatccca taccattgaa
    12241 ccagcttctt cagtagaaaa ttcttcattt tttttaaaac ccagcttttc ataaaaagca
    12301 acggaacgat tgacatcagc aacaggaaaa ttaacaaaaa ccatactatt catttgtttc
    12361 gtctcctttc tgttttgttc gtctatcatc tcttatttag gaaacgc
//
LOCUS       DAZULY010000076        12205 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000076 DAZULY010000000
VERSION     DAZULY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.077"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..216)
                     /locus_tag="ACG81A_001335"
     CDS             complement(37..216)
                     /locus_tag="ACG81A_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014706.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(232..432)
                     /locus_tag="ACG81A_001336"
     CDS             complement(232..432)
                     /locus_tag="ACG81A_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014707.1"
                     /translation="MNKIIPLLFVLFVGEILMRHGAALHLNFVVFLGILVFCLIFWRF
                     VLTLVSLIFKLIAAIFLIGLFR"
     gene            complement(597..1304)
                     /locus_tag="ACG81A_001337"
     CDS             complement(597..1304)
                     /locus_tag="ACG81A_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014708.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="ACG81A_001338"
     CDS             complement(1297..1734)
                     /locus_tag="ACG81A_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014709.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="ACG81A_001339"
     CDS             complement(1749..2000)
                     /locus_tag="ACG81A_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014710.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="ACG81A_001340"
     CDS             complement(2000..2206)
                     /locus_tag="ACG81A_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFS5014711.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="ACG81A_001341"
     CDS             complement(2643..2903)
                     /locus_tag="ACG81A_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014712.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            3077..3181
                     /locus_tag="ACG81A_001342"
     CDS             3077..3181
                     /locus_tag="ACG81A_001342"
                     /inference="COORDINATES: protein motif:HMM:NF028245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFS5014713.1"
                     /translation="MPVETALGLMISFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="ACG81A_001343"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="ACG81A_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS5014714.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="ACG81A_001344"
     CDS             complement(3810..4709)
                     /locus_tag="ACG81A_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFS5014715.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="ACG81A_001345"
     CDS             complement(4722..5315)
                     /locus_tag="ACG81A_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFS5014716.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5391..5540)
                     /locus_tag="ACG81A_001346"
     CDS             complement(5391..5540)
                     /locus_tag="ACG81A_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014717.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5556..7760)
                     /locus_tag="ACG81A_001347"
     CDS             complement(5556..7760)
                     /locus_tag="ACG81A_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFS5014718.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="ACG81A_001348"
     CDS             complement(7771..9231)
                     /locus_tag="ACG81A_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFS5014719.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="ACG81A_001349"
     CDS             complement(9292..11664)
                     /locus_tag="ACG81A_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFS5014720.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            12074..>12205
                     /locus_tag="ACG81A_001350"
     CDS             12074..>12205
                     /locus_tag="ACG81A_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS5014721.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT         3040 a         2531 c         2053 g         4581 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtccgt ttcctcctcg taaaatatca tttgtctaat
    11701 agtttttatt caaaatgttt ctagattata aaatttattg aataacacat tcctaacgga
    11761 atttgtttcg atattgtgtg atttctcaga gtacaatgga tgtatggaaa ggatccaacg
    11821 ggaatttgct atttcctcct gtagcgtttg tctaccctgt ttagtctgtt acccgaacca
    11881 ctgttatcac taccaactca ttagctgttt cgataatgga ctagactttc aaatagtagt
    11941 ttggaaggca gcttctagct cttttatcac cgtattctat taggcgaggt tgtggctata
    12001 cagtgagttt ctgggaaatc ccacagtatc gaagcatttt ccaaaattta tatcgaaagg
    12061 aggtccttct tccatgaaat tatttgtcgg tattgacgtt agctctgaaa aactagatgt
    12121 ttgtttttta acagacgaca atcaattgtc tatcttatct gaaatctctg ttgccaatga
    12181 catcgaaggt gcttcattca tacga
//
LOCUS       DAZULY010000077        12124 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000077 DAZULY010000000
VERSION     DAZULY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.078"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..573)
                     /gene="frr"
                     /locus_tag="ACG81A_001351"
     CDS             complement(16..573)
                     /gene="frr"
                     /locus_tag="ACG81A_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFS5014722.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(576..1298)
                     /gene="pyrH"
                     /locus_tag="ACG81A_001352"
     CDS             complement(576..1298)
                     /gene="pyrH"
                     /locus_tag="ACG81A_001352"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFS5014723.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1434..2315)
                     /gene="tsf"
                     /locus_tag="ACG81A_001353"
     CDS             complement(1434..2315)
                     /gene="tsf"
                     /locus_tag="ACG81A_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFS5014724.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2414..3196)
                     /gene="rpsB"
                     /locus_tag="ACG81A_001354"
     CDS             complement(2414..3196)
                     /gene="rpsB"
                     /locus_tag="ACG81A_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFS5014725.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3555..4034
                     /locus_tag="ACG81A_001355"
     CDS             3555..4034
                     /locus_tag="ACG81A_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS5014726.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4251..5342
                     /gene="phnW"
                     /locus_tag="ACG81A_001356"
     CDS             4251..5342
                     /gene="phnW"
                     /locus_tag="ACG81A_001356"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFS5014727.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5335..5463
                     /locus_tag="ACG81A_001357"
     CDS             5335..5463
                     /locus_tag="ACG81A_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFS5014728.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5467..6012
                     /locus_tag="ACG81A_001358"
     CDS             5467..6012
                     /locus_tag="ACG81A_001358"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFS5014729.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6466..8157)
                     /gene="argS"
                     /locus_tag="ACG81A_001359"
     CDS             complement(6466..8157)
                     /gene="argS"
                     /locus_tag="ACG81A_001359"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFS5014730.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8577..9524)
                     /gene="arcC"
                     /locus_tag="ACG81A_001360"
     CDS             complement(8577..9524)
                     /gene="arcC"
                     /locus_tag="ACG81A_001360"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFS5014731.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9639..10658)
                     /gene="argF"
                     /locus_tag="ACG81A_001361"
     CDS             complement(9639..10658)
                     /gene="argF"
                     /locus_tag="ACG81A_001361"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFS5014732.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10749..11978)
                     /gene="arcA"
                     /locus_tag="ACG81A_001362"
     CDS             complement(10749..11978)
                     /gene="arcA"
                     /locus_tag="ACG81A_001362"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFS5014733.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3310 a         2518 c         2194 g         4102 t
ORIGIN      
        1 gtttttatga ttttattaaa cttccaataa ttctttttct ttatcagcag cgatcttatc
       61 tacttctttg atgctgttat ctgtcaatgt ttgtacgtct ttttctaatc cgcgcaaatc
      121 atcttcggta atatcaccat ttttttgttg ttttttatat tcatcgatcg catcacgacg
      181 gatgttacga atcgcgattt ttgcattttc tgcttctttt ttaacatctt tagctaattc
      241 ttttctgcgt tcttctgtca gttgcgggat aactaagcga atgacatttc catcattggt
      301 tggactgatt ccgatatcac ttgccataat agctttttca acatcttgaa gaatgctctt
      361 gtcaaaaggt gtgatcatta aaacacgtgc ttctggaatt tggattgatg ccatttggtt
      421 caatggtgtt ggcgcaccgt agtagttcac agtaatacgg tccaaaaggc tagcgttcgc
      481 acggcctgcg cggatttgcc caagttcacg ttgaagattt tgtgctgctt ttttcatttt
      541 ttcttttgct tctgtcataa ttgcatctgc catttttatt tcccccttac agttgttccg
      601 atattttcgc ctaagattgc acggcgaata tttccgtgtt catttaaatt gaagacaagc
      661 agtggaatat cattatccat gcttagtgaa cttgcagttg aatccatgac ttgtaatcct
      721 ttggcaataa cttccagatg agtcagttct tcaaatttta ctgcatttgc atctactttc
      781 ggatcagcag aataaacacc gtctacattg tttttagcca tcaggataac atctgcgccg
      841 atttctgccg cgcgtaatgc tgctgttgtg tctgtcgaga agtacgggtt gccggtaccg
      901 cctgcaaaga taacgatccg tcctttttct aaatgacgtt ctgctttacg gcgaatatat
      961 ggctctgcga tttgacgcat ttcaatcgaa gtttgcacgc gtgtcggtac acccacgttt
     1021 tccaacgtat cctgtaaagc taaggcattc ataactgtag ctaacatacc catgtaatca
     1081 gcttgagcac gttccatgcc catttgtgct ccgatttgtc cgcgccagat atttccgccg
     1141 cccacaacga ttgccatttc aacgcccagc tcgtgaactt ctttgatttc ttggatgatt
     1201 tctttgatta ctggtggttt gattccaaat cctgcgtccc cagctaaagc ttctccactc
     1261 aattttaaga ctacacgctg atatttcggt gtgaccatgt tcattcctcc aatattttct
     1321 attgccattt taccatactt agatgatttc gacactctaa gactggtttt tcttgttttg
     1381 cttaaaaaaa gggagcgcac aaaccgtgcg ttcccgtctg ttattcaaca gaattatttt
     1441 ttcacttggc tcataacttc ttctacaaag ttatcttcgc gtttttcgat accttcgcca
     1501 acttcgaaac gtacgaatgt tttaacagtt gcgccttttg atgcaacgta tttttcaaca
     1561 gtcatatcag gatctttaac gaatggttgg tccaccaaag cgatttctgc tttgaatttc
     1621 ttcaagcgtc cttctaccat tttttcaacg atattcgctg gtttgccttc gttcaatgct
     1681 tgttctgtaa gaacagtttt ttcatgttct aattcagctt ctggaatttg tgattcgttt
     1741 acataacgag gattgatagc tgctacgtgc atagctacgt ctcttgcaac tgtttcgtca
     1801 gttgttccat ctagaacagt caatacggca atacgtccgc ccatatgcaa gtatccacca
     1861 aatgcagcat tgtcgtcttt ttctacaact tcaaagcgac ggaagctgat tttttctccg
     1921 ataacttgag ttgcttcgat caagtcagat tcaattgtac ctttatctgt tttgattttc
     1981 attgcagctt ccatgtctgc tggtttgttt tcagcaacaa gttctgcaat ttcttttact
     2041 aaatcttgga acatttcgtt tttagaaaca aagtctgttt ctgagtttac ttcaacgatt
     2101 gctgcagtgt tacctttcac agcaactgaa gccaatcctt cagctgcaat gcggtcattt
     2161 ttcttcgcag cctttgccat tcctttttca cgtaaaagat caactgcttt ttccatgtcg
     2221 ccttctactt caactaatgc ttttttcgca tccatcattc ctacgccagt catttcgcgt
     2281 agttctttta ccattttagc tgtaacgtct gccatgttta actcctcctg tgtgtttttc
     2341 tttttaaaaa aagctgcttc aaaggagagt gaggcttgtc gcccagcctt tgaaacagct
     2401 ccatcattca aaattattct gctgaagaat tgtcgccttc aacaacatct acgatttctt
     2461 cgattgatgt tgctgtgttt tcttctgtga aatcttcttc aactacttga tcttcacctt
     2521 ggtttccttc gatgaaagca tcagccattt ttgaagtaat caatttaacg gcacgaattg
     2581 catcgtcgtt tgaagggatt actacgtcga tttcatctgg atcgcagttt gtatcaacca
     2641 tagctacgat tgggatattt aatttgtgtg cttcttgaac agcaatacgt tctttacgtg
     2701 ggtcaacgat gtacattaca tctgggattc ttggcatatc agcgataccg cctaagaatt
     2761 tttcaagacg ttcacgttct ttgttcaaac cagcaacttc ttttttaggt agaacttcaa
     2821 aagttccatc ttcttccatt gcattgattt gtttcaaacg gctgatacgt ttttggatag
     2881 tatcccagtt tgtcaatgtt ccgcctaacc aacgatggtt aacgaagtat tgtcctgcac
     2941 gagttgcttc ttctttgatt gcttcttgtg cttgtttttt tgtacctacg aataatgcta
     3001 cgccgccttc ttcagcaacg ctcttcatgt aatcataagc tgcatctact aatttaactg
     3061 ttttttgcaa gtcaatgatg tagattccgt ttctttctgt gaagatatat ttcttcattt
     3121 ttggattcca gcgacgagtt tggtgaccaa agtgtacgcc ggcttctagt aattgtttca
     3181 tagaaattac tgccatgttt gtttcctccg atttggtttt atttttcctc ttcttatctt
     3241 ctaccttgcc ggcaaacttt ttcaagcacc taccgacaat ccaataagaa tgtggatttg
     3301 ctgatgcata aagcaccctc agctattata cagacaaaag atccgtatcg caagagcaaa
     3361 tatacttttc tgctcaacat tcaatagttt acctgaaaga taaaaagaat tcaagaaaaa
     3421 cagtagattt ctctttgttt ctttattatc ctttttccat aaaataattt taggtataaa
     3481 agacgaatat tattgggcaa tccttctttt ttttgttatg atacatgtga aatctttcgt
     3541 aaaggagaaa gaaaatgcgc aaagctcaaa gacatttgct gattaaacaa gtcattgaag
     3601 aattcccgat ccgtacgcaa gaagaactat taaacaaact tgggcattat ggcgttaccg
     3661 ccacgcaggc aacgatttct cgagatatcc gtgatctaaa aatcgtgaag gcacctgatg
     3721 aaaatggcgt ttcccgcttc gtattgtttc gcgaaaaagg aactcctgag actaggactg
     3781 aggaagagga tcggctggtt caaatgattg aagacattgt attgaaagtc gaacgagtcc
     3841 actttttgac gatcgtcaat accttgccag acaatgctca tttatttgca tctgttttag
     3901 atgagatcaa acctccctct attgtcagta caattgctgg attcgatacg atcatggtca
     3961 tctcggaaaa tgaggaggac gcaaaacgag tagaagaatt tcttcaccat ccaactgaag
     4021 tatcactttt ttaatcagag cacgtattcc taatatggaa tgcgtgtttt tttattttca
     4081 atattttcct tattgaataa acctttacac tctttacaaa aatacatcat tttttattca
     4141 aatttttcaa caccttgatt gaatcacaat aatcatcatt tatattggat gttaacaaga
     4201 gcagcttctt gctctttgtg tatttcaaaa aagaaatgaa aggaaatcat atgtataaat
     4261 tattaacttc tggaccattg acgacttctg aaacagtgaa aaatgctatg ctgatcgatc
     4321 attgtacttg ggatcaagaa tacaaagaaa ttacacaatg gataagaaaa aaattattgg
     4381 atcttgctca agtatcggaa ggaatataca caacggtctt aatgcaaggt agcagctcct
     4441 tcggggtaga atcggtatta agttctgtga tcaggccaga agatactgta ctgatttgtt
     4501 caaacggcgc atatagacaa cgaatgatca acatgtgcga atgtctgaaa ctgaactata
     4561 ccgtttatga ggtatcggaa catcaaatcc tccaagctca tgtgattaca cagctgattg
     4621 aagagaatcc gtctatctcc gcactagcaa tggtccatag cgagactact tccggtattt
     4681 tgaataattt ggaagagatt gctgctgtca cacaagaaag agatcttttg tttatagtgg
     4741 acgcgatgtc aagctttggt gggataccga tttgtgtagc cgaactaggg attgattttt
     4801 tgatttccag ctcaaacaaa tgtattcaag gtgttccagg cttttctttt gtacttgcta
     4861 ataaagcgga actggaaaaa cgaaaaaatg ttagccgaag tttgtcactt gatctatatg
     4921 accagtaccg cgtgatggaa aacgagaatg gcaaatggcg tttcacttcc cctacccata
     4981 cggttttggc ttttcaacaa gcgctgaagg aactagaggc agaaggagga atcgaaactc
     5041 gatattcacg ttatctcgcc aataatcatg tgctacgaca aaaaatgaca gagatggggt
     5101 tccagccttt cattcaagaa tatcaggggc cattcatcac ctcttttctt taccctaagg
     5161 actggcgatt tgattttaat catttttatc agtatttaaa gcaaaatgga tttgtgatct
     5221 atcccgggaa aatcagcgat catgaatgtt ttcgtatcgg aaatattggc gagatctatc
     5281 cggaagatat tttggaactg acagcaatta tcaaagacta taaggagaac gttaatgatt
     5341 aaaactgtaa tttttgattg ggcaggaaca actgtggact ttggctgtat ggctcctgtc
     5401 cacgctttta gaaatgcttt tctagaaaaa ggaatccaac tgactgacaa agaaatccgc
     5461 taaccgatgg gaaaattaaa atgggatcat atccaacagc ttctttccct cccttctgtc
     5521 aaaaaacaat ggatagctat ttatggacaa ctacctcagg aaaaagaggt ggaggaattg
     5581 tatcaacaat ttgaacatta tctgtttaaa gatttggtca agcattctac cttgaaaccc
     5641 gacacattgg aaacgatcga acggttgaga aacgctggga tcaatattgg ttccacgaat
     5701 ggatttaatg ctcctatgat gacaatcgta gcagaaaccg caaaaaaaga aaggtattca
     5761 ccagacttca tggcaacttt tgaagacgtc gaagggtatg gtcgaccgtt tccttatatg
     5821 atccataaaa acatgcagta cttcaaaaac aagtcggttg atgaagtaat caaagtcgga
     5881 gatacggtct cagatatcca ggaagggaaa aatgccggag tgttgacagt aggtgtaatt
     5941 gaaggtagtt cgctaatggg gatttcttat gatgagtacc aaaaattaga gatggggaaa
     6001 aagatcgttt aaaacagtct gttgctctta gattccaaca agccggcgct gatatttgtg
     6061 taaacaattt aaaagagtta acagaataca ttttgtacgc tgacatgaat ccagaatgac
     6121 ttttatacaa aataagaggt tgtgaaaaag ctgtcctgta tcaagtaata aaaacagcca
     6181 agcaaaaata gctcctcata tttttcgttt ggttaggctt attaccgcag atgcaagctt
     6241 ttgaacaccg tttattcgaa aaaaaaaagg gagctgtgac acgacttttg tcacagtccc
     6301 cttatttgca aacaaacagt gtttaagaga caacctctcc actaaacggt gttcattcag
     6361 ctgactctcg gtatttgctc aaaaaacgat aaaatttgaa aagctatttt ctcttttgtt
     6421 tctctaatac tcggatcaga acgctgattt cacattctct ttttcttaca tcttatctgg
     6481 tgcatgaagt cctagcaaac gaaggtcttc tttcaataat acagtcactg cataaactag
     6541 agctaagcgt gcttcttttt gttcatcatc agctaaaatc ttcgtatgag catagtattt
     6601 gttgaatgct tgtgctaatt tgattgcatg tttcgcaatc actgacggtt cgtatttttc
     6661 cccagcagac aagaccgttt cagggtattt ttgaactaat ttaaccactt cccagctggt
     6721 atcgtcattc aatgcatagt ctgctgtttc tgaagggaca aatcctgctt tttccaacag
     6781 gctgactgca cgagcatgtg tatattgcac ataaggtcca gtttctcctt caaatcgaac
     6841 aacttcttcc aggttaaagt caaatgtatt cagtcgatcg tttttcaaat cgtggaaaat
     6901 gactgcccca accccaactt gtttagctac agcatctttg ttttccaaat cagggttttt
     6961 ctcgctgatt tgttctttag cagattggat tgcttcattt aatacttctt ctaataagac
     7021 gatctttcct ttacgagttg ataatttttt accaccttgc gtgatcaaac caaatggaat
     7081 atggtgcatg tcgtcagacc agtcaaagcc catttccttc aagacagctt tcaactgttt
     7141 aaaatgatag ctttgttcat tccctactac atatagagat tgtttgaagt cgtaagtacg
     7201 tttacggtac aatgcagcag ctaagtcacg tgtaatgtat agtgtcgcac cgtctgactt
     7261 cttaatcaac gctggattca ggtcataagc agataggtca acgatctctg cacctttatc
     7321 ctcgttcagc aaatgttttt cttctaaaag tttgacgatt tcatccatct tgtcattgta
     7381 gaaagcttca ccgttcaacg aatcgaaacg tacttccagc agatcataga ttttattgaa
     7441 ttctttcatc gattcatcac ggaaccattg ccataattga atagcctctt tatcgccttc
     7501 ttccagacgt ttgaaccaag cacgagcttc ttcatttaat tctggttctg tttcagctac
     7561 ttcatggaat tggacgtata aacgaagcaa ttcattgatt ggttctgctt taacagcttc
     7621 ttctgtaccc cattttttgt aagcgacgat cagttttcca aactgtgttc cccagtcacc
     7681 taaatggttg atgcggattg gctgataacc gattttttcc ataatgaaac cgatcgaatt
     7741 accgataact gtcgaacgca aatgtcccat tgagattggt ttggcaatat taggagaaga
     7801 catgtcgatt ggtactgttc cttgattccc gatgttgcta tccccataat gttctttttc
     7861 tttcactact gtttgcaaaa cttttttgct gataagttct ttattcatga aaaagttcaa
     7921 gtaaggtccg acaacttcga ttttttcaaa gttggcagaa tcgatttttt ctgctaaatc
     7981 tgcagcgatt tgctgtggcg cttttcggta aacttttgct aatgaaaagg ctggaaatgc
     8041 aacatctccg tgttcagctg attttggatt ttctagtaat tgctcgactt gttccagtgt
     8101 taaatcatct ttgacagcgt catagacagc ttttgcgaca agatctttat tattcatctt
     8161 tattcctcct aaagtgttcc cttgattgtt aaacaaaaaa aatctctagc gacggcatac
     8221 ttagtatgcc atcactagag acgagatttc ccgcggtacc actccaattg tcctaaaaaa
     8281 ggaccagctc catctgatat cggtagacga ccgtctgttt aaaacagaat tctccaagtg
     8341 cgcttcgttg tatcttcttc attcggcttc caccacttcc gaattcgcta gccaagaatg
     8401 atacaagtac tctcttgttc aacaaatctt agtcttatta aactttcttg aattatatca
     8461 gaaaaaaaaa cgaaaggcta gtcgtttcat aaaaaaacaa tccagatggc ctaaaggaga
     8521 agaaatacca cctggatagc tataaatcat ttcgtaaata cgaaagactt aacaaattaa
     8581 tcagctacga taatcgttcc ttcttcagaa gctaaaagat tctcgatatt ttctaaagaa
     8641 gtgatgattg ccttagcgtt tggttttgct tcaacaaatg caatagctgc ttctactttc
     8701 ggaagcatgc ttcctggagc gaattgcttt tcatcaatgt attgtttcat ttctgaaaca
     8761 gtaacagttt ctaatttctt ttgatctggt ttttggtaat tgacatacac attgtctact
     8821 cctgtcaata cgatcagcaa atccgcatcg atgatttcgg ctaatttttc tgaagcaaaa
     8881 tctttatcga ttacggcttc tcgtccttct aatccagtag ctgtttctac aacaggaatc
     8941 ccaccgccac caacagagac tgtgataact ccttgatcga ccagcgtttc gatgacacgt
     9001 gcttctttaa tcgagatggg tttaggggaa gcgactactt ttctccatcc acgtccagca
     9061 tcttctttga atgtcacagt ggagtcagca ttcatttgtt ctttggcttc ttcttccgtg
     9121 tagaatggac cgactggttt acttggattt ttgaatgaag gatcattttc atccacaata
     9181 acttgagtga ctaaagaaac cacatcttta tcgatccctt tttccttcag tacttcgccc
     9241 atggcgtttt gtaaccagta accgatagat ccttctgtca ttgcaacaca tgtatctaaa
     9301 ggcattgccg gtgttttctc agaatcagca gcttgctgct gaatcaatag gttaccgact
     9361 tgcggaccat ttccgtggga gatgatcaat tcatctcctt gttcgataaa tttcactaaa
     9421 tactttgctg tttccattaa cgcttcttgt tgggcttttg cacttgcatc cgtagacaag
     9481 atggcattgc cccctaaagc gacaactact tttcgtttgg ccatttcatt cgtcctttct
     9541 tcagttctac cgaattttct agataacaaa acgagccagc gttttgtgat tttccctcaa
     9601 tgctgactcg ttttttcgtg agatgaatgt ggttatgctt aaacgcgagg gataaacaag
     9661 ttacctaatg ttgctgccat gatcgctttg attgagtgca tacgattttc tgcttggtca
     9721 aattgacgag catatttgct gcggaagact tcatctgtta cttccatttc agtgatgcca
     9781 aagcgttctt tcatttgttc accgtaaaca gtgtttgtat catggaaagc tggcaagcag
     9841 tgcaagaaga tcaaacgatc tgtgttgtgt gttttttcta ccatttccat attgatttgg
     9901 taaggtttca acaatttgat acgttcttcg aatttatctt cttcacccat tgatacccat
     9961 acatcagaat aaaggacgtc tgctccgtct acacctttat caacatcgtc ggtaatcatc
    10021 aattgtgcac cggatttttc agcaaagcct tctgccattt tgacgatttc ttcttcaggt
    10081 tgtaattctt ttggtgcaac gatacgcata ttcgctccta aaattgcacc tgtgactaat
    10141 aaagagttag ccatattgtt tcgaccatct ccgcagtaag caacagtgat accttctact
    10201 gttccaaagt tttcttggat agttaaaaag tcagcgatca tttgtgttgg atgccattca
    10261 tcagtcaagc cattccaaac tggaacacca gagaattctg ctagttcctc taccattttt
    10321 tgactgaatc cacggaattc gattccgtca aacatacgtc ctagtacttt agctgtatct
    10381 tctgtggatt cttttttacc taattggata tcatttgcac ctagatattc aggatgtgca
    10441 cctagatcga tcgctgcagt agtaaatgca gaacgagtac gtgtagatgt tttttcaaat
    10501 aaaagcgcaa tattttttcc ttctagatag tgatgtggga taccacgttt tttcaagtct
    10561 ttcaagtgtt ctgaaaaatc gatcaaatat tgtaattctt cttttgtaaa gtctttttct
    10621 gctaataagc ttcttccttg gaatactgat tctttcatat taaacattct cctttagatt
    10681 gtttttattt aagattaaag aggaaatccc gacggcagct gacagtaaga ctgccgtcag
    10741 gaaacaaatt acttcaagtc ttcacggaca agtggttggc tcatgcagcg tggaccacca
    10801 cgacctcgtg acaattcact tgaattgatt tctaacactt tgatgccata gctacgaagc
    10861 aattcgttcg atacataatt tcggttataa gtgactacaa cacctggagc gattgccaat
    10921 gtgtttgatc catcattcca ttgttctcga ggtgcaacga tctcatctcc attaccagtt
    10981 gggatcagta caagatcatc taatccaaga gcagctttca tcgtttcgtg taagttatct
    11041 gaatgagtaa tcttgatatc atcaccatct ggttcgattg taaagacatc catcttaccg
    11101 tctttacttt gaattgctgg atggatagtg aatttatcgt gatctaccat tgtaaatacg
    11161 gtatccaaat gcatcattgc acggttgttc ggaattttga ttgccaacac tttttcaaat
    11221 cctgaatttt ttgcgaatag gtggcgtgct aatttttcta aagctttcgc attagttcgt
    11281 tgagaaatcc caacagctac tactttatcg cttaagatca attcgtctcc gccttcgata
    11341 tgatctggat tttcacgatc caaccatact tcgattcctt tatttgcaaa tcttggatga
    11401 tgtttcaaaa tatattctgt aaacatcgat tcacgacgac gtgcttcaaa agtcatctta
    11461 ttgactgtca tcccatttcc cattgatgcg gaagggtcgc gtgtaaagta caagtttggc
    11521 attgggtcca tgtaaaatgg atagtcatcg tctgctgaca gatcatatag actgctagaa
    11581 cgtacttcga tatcttttgt acgaacccca gccatgattt tatctaccat ttcttgcgtt
    11641 tccattgata gaaggaactc atgaagaccc tctcttacag cattcgatgc aatatgagat
    11701 tcatccaaca ttttattcaa gaattgttct ttgacattgc ctgcgtcaat cgcttctgcc
    11761 gctaattttt ccaagtaaag agtctcgacg ccttcatttt gcaatgtctt cgcaaagttg
    11821 tcatgttctt cttgagcaat cggaagatat ggaatatcat cgaacaacag acgatccata
    11881 atatctggtg tcaaattttc cacttcttgt ccaggccgtt tcagtaaaac tgtctttaat
    11941 ttccctattt cagagaaaac gtgaataggt ttatccatgt tgtgttgcct ccttcttttt
    12001 gattacatgt ataggatatc ctccttttgt aatcactttc ataattttgt ctcattcaaa
    12061 atgagacatt cctcacatta atattcgata attaaataat taaacgtttt tctattttta
    12121 agcg
//
LOCUS       DAZULY010000078        12105 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000078 DAZULY010000000
VERSION     DAZULY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.079"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(56..1615)
                     /locus_tag="ACG81A_001363"
     CDS             complement(56..1615)
                     /locus_tag="ACG81A_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFS5014734.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            1770..4004
                     /gene="pcrA"
                     /locus_tag="ACG81A_001364"
     CDS             1770..4004
                     /gene="pcrA"
                     /locus_tag="ACG81A_001364"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFS5014735.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            4021..6057
                     /gene="ligA"
                     /locus_tag="ACG81A_001365"
     CDS             4021..6057
                     /gene="ligA"
                     /locus_tag="ACG81A_001365"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFS5014736.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            6163..6468
                     /gene="gatC"
                     /locus_tag="ACG81A_001366"
     CDS             6163..6468
                     /gene="gatC"
                     /locus_tag="ACG81A_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFS5014737.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            6468..7940
                     /gene="gatA"
                     /locus_tag="ACG81A_001367"
     CDS             6468..7940
                     /gene="gatA"
                     /locus_tag="ACG81A_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFS5014738.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7937..9367
                     /gene="gatB"
                     /locus_tag="ACG81A_001368"
     CDS             7937..9367
                     /gene="gatB"
                     /locus_tag="ACG81A_001368"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFS5014739.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            9377..10432
                     /locus_tag="ACG81A_001369"
     CDS             9377..10432
                     /locus_tag="ACG81A_001369"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS5014740.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            10543..11916
                     /gene="rlmD"
                     /locus_tag="ACG81A_001370"
     CDS             10543..11916
                     /gene="rlmD"
                     /locus_tag="ACG81A_001370"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS5014741.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            11919..12047
                     /locus_tag="ACG81A_001371"
     CDS             11919..12047
                     /locus_tag="ACG81A_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS5014742.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         4189 a         2232 c         2629 g         3055 t
ORIGIN      
        1 atagaatcaa gtagtgtaga tgaatagttg ttgtaaaata gtttttccat gttcgttaaa
       61 atgattgctt caaatttgtg atagaagatt tagcctcgat ttcctgcttg attcttggca
      121 agtttgcttc atcagtcaag ataaccaaaa tatctcctac attcatgatc gtatcaccgt
      181 gagtcaagat ttcctgattt cctctgcgta tagagattag cagcatattt ttaggccaat
      241 tgaagtctcg taccattaat ccgtctaaag aactctctgc cgttactgga aaatcaaaag
      301 ttgtccttgc accttttagt gaggcggtct gagtatttcc aagtagacgt tccaataatg
      361 attcgtaaat tggattaccg ccgagaaggt cattgacgat ataagcaaca agagcagcca
      421 atcctaaagg catcagatga ttaagagagc caaccatttc tgtcactaaa ataattgctg
      481 tcagcggtgc ttttccaatc gctgtgaaat aaccagccat ggcaataacg acaaaatcct
      541 tgatgtagat tggatccatc ccgaaatcaa ccataatgtt tccataaaag ctaccgatta
      601 aagcccccaa tgtcaatatc ggaaggaaaa tcccccctgg aagaccccca ccgtaagaaa
      661 tcatggaaaa aacgaatcga agaacaaaca aaccgataat cataatgatt ggcaaggaag
      721 catgtccaaa agctaaaacg atttgattgc ctccgcctag agtactaggc cataaataac
      781 caattggcaa aatcagcaag aaaggaacga acccgtgaaa ataagctggt aaaggcaatc
      841 ggttgtaaaa ttttggcaaa gccagcaaga cattctgata aataaaacct aagatgccta
      901 gaagtactcc taagatcact aaggaaccat aatatttcaa cggcaaagaa ggaaggtcac
      961 ctagatataa aacaggtctc aagccgaaaa agtaaagaga gacgaagtta gcagtgattg
     1021 ctgaagcaaa tgatgtcagc caaacaagtg gagaaaagga gtgatggatt tcttctaaaa
     1081 caaagagtaa tcctgcgata ggagcattga atgctgctgc aagtccgcta cttgcaccgc
     1141 tagaaattaa aatcttttct tctgaagatg gactccgaaa caattgtcca gctccttgtc
     1201 caatggatgc gccaagctgt atagaaggtc cttctctacc tagaaaaagg cccgcaccaa
     1261 ctgaaagtac accgccaatg aactttttcc ataaaacaga gaaccagttc attgataatt
     1321 ccccgcgaag ctgtccctct acttgtggaa tcccgcttcc cttgatatcc ggttcatttt
     1381 tgaccagata acccacgaaa aatgctgcaa ggatgcagca aatagctagg ccaattaagt
     1441 aaatcggctg ttcccgactc atttcataga tacttcgaac taaatcgctt agcttttcga
     1501 tagataatcg aaacaggcta acgacgattc ctgtcagcag tccaacaaat attcctttta
     1561 gaataaaata tatctgagtc ttatctaatt tcttgatttt tccttttttt tccattcaaa
     1621 cgcctcgctt tcttttaatt tagcgcgaaa tcaacgaatt gacaaggaaa aatccataaa
     1681 aagaaacaaa tgattttgaa ctagctagtt taatgaaagc caagaaaatg ctataattaa
     1741 caagattgat aaaagaaagg atgttgttaa tgtcatccaa agctgaatta ttaaacggga
     1801 tgaacccccg tcagaaagaa gctgttcttc acactgacgg accattactg ctaatggcag
     1861 gtgcaggaag tggaaagacg agagtgctga cacaccgcat tgcctatttg attgaggaaa
     1921 aagaagtcaa tccttggaac atcctagcga ttacttttac caataaggct gcaaaagaaa
     1981 tgaaagaacg tgtcaatgcg attctcgcat ctggaggcga agatgtctgg gtatcgactt
     2041 ttcactcgat gtgtgtgcgt attttaagaa gagatgttga ttttatcggg tataaccgga
     2101 actttacaat catcgattct tctgaacagc tgaccttgat gaaacggata ctaaaagaac
     2161 tgaacattga tccgaaaaaa tatgatcctc gaagtatatt aggaacaatc tctcaagcaa
     2221 aaaacagcct gcagacccct caggattttg caaaaatgca aggcagttat tacgaagaga
     2281 ttgcggcaaa atgttatgct gcttaccaaa aagagcttca atataatcaa tgtatggact
     2341 ttgacgacct gatcatgaat acgattcgat tatttgaaga acatcctgat tcactcacgt
     2401 attatcaaaa caaattccat tatattcatg tagatgagta ccaagataca aatcacgcac
     2461 agtatacgct agtcaattta ttggcaggta gatttaggaa cctgtgcgtc gtgggagatg
     2521 cagatcaaag tatttacggc tggcgcggtg cagatatgca gaacatcttg gattttgaaa
     2581 aagattatcc agatgctgca gtaattttgc tagaacaaaa ctatcgctcc acgaaaaaca
     2641 tccttagcgc agcaaaccaa gtcattgaaa acaatagcaa tcgaaaacca aaaaacttgt
     2701 ggacagaaaa caaagaagga aacaagatca cttactaccg tgcagataat gaacgagacg
     2761 aaacccgttt tatcgtagac cggatgcaag aagagattcg aagcaatcat cggaattacg
     2821 gcgactttgc catcttgtac cggacgaacg cacaatctcg tgtaatggaa gaaactctgt
     2881 tgaaagcaaa tattccttat aaaatggttg gagggcacaa gttctacgat cgtaaagaaa
     2941 tcaaagatat tttagcgtat ttgaatgtgc tggtaaatcc tcaagattca atcagttttg
     3001 agcgtatcgt taattcgccc aaacgaggca tcggaccagg atctatcgaa aaactacgca
     3061 gttttgcttc tcttcatgaa tggccactgc ttgaagcagc gcaaaatgtg gatctggcaa
     3121 atatcggtgg aaaagcagga cagcaattag gtgcatttgg agagatgatc caagaagtca
     3181 ctcagatgat cccatacttg acagtgacag aattgacgaa agaggttttg gatcgtagcg
     3241 gatatctaga agacttaaaa atccaaaata cattagaagc acaagcacgc atcgaaaact
     3301 tggaggaatt tttaacggtc acacaagaat tcgacaaaca attcgaacag caaaatgaag
     3361 aagacgctga tgcaccagaa gaaaaactaa cggtcttctt aaatgatctg gcacttgttt
     3421 ccgacattga caatttagaa gaagatgctt cccaagtgac attgatgact ttgcatgcag
     3481 ccaaaggatt agaatttcca gttgtattcc tgattggtct agaagagggg gtttttcctt
     3541 tatctcgagc attgatggaa gaaagcgaac tggaggaaga acgccgtctc gcatatgtgg
     3601 ggatcacgcg agcagaagaa gcgttgtatt taacaaatgc tttctccaga acattatatg
     3661 gacggacgca atataaccga ccttctcgtt ttgtagaaga aatcgatcag gagttactgg
     3721 aaatcgaagg catgcgtcct acgcctaaaa aaacgcctgt attcgctaaa aaaacagcat
     3781 atagctataa acagccagag acagcggttg ttccctctaa aagtgctact ggaggggaaa
     3841 ataacagctg gaaacctgga gataaagtca aacacaaaaa atggggtcta ggaacagtcg
     3901 tccgagtaag cgggacgtcg aaggatctag aactagatgt ggctttccca agtcaaggag
     3961 taaaacgtct tttggctgcc tttgcgccaa ttgaaaaagc ttagtagtgg agggtaaatg
     4021 atggaacatg aaatgacgat tgacgaagct gctaaacgag cagaagaact tcgtacaaga
     4081 ctgaaccaat ggtctcgcga atactacgta gaagataaac caacagtgga agactatgtt
     4141 tatgataaag aatacgcaga attagtagca atcgaggaac aatatcctga tttgatcacg
     4201 tctgattctc ccactcaacg agtaggcgga aaagtattag aaggttttga aaaagtcact
     4261 catgatattc cgctttacag tttgaacgac gttttcagca aagaagagct gatagctttt
     4321 gatcaacgtg tacaaaaagc agtgggacgt gtagttgact attgctgtga actaaaaata
     4381 gatggacttt ccgtttcttt gcgttatgaa gatggaaatt tcgtgagagg tgcgactcgc
     4441 ggggatggaa ctgtaggaga aaacatcact gaaaacctaa aaactgtccg gtcggtccca
     4501 atcaaattaa aagaaccaat gaacatcgaa gtaagaggcg aatgtttcat gccaaaacgg
     4561 tcatttgtcc aattgaacca agacagagaa gcagagggaa aagatatctt tgcgaatcct
     4621 cggaacgccg cagctggttc tttgcgtcag ttagattcta aaatcacagc gaaaagaaat
     4681 ctggacactt ttttatacac agtagctgat ttcggtccta tgcaagcaaa aacccagtat
     4741 gatgcgctag aagagctaga aaaaattgga ttccacacga atcgggaaaa acgtctatgt
     4801 cattcgattg atgaagtgtg gtcatacatt gaagaatatc atgacaaacg tgtggatttg
     4861 ccctatgaaa tcgatggaat cgtcatcaaa gtgaatgaat tctcgctaca agatcaactt
     4921 ggttttacta tcaaagcacc gagatgggca gcagcttaca agtttccgcc agaagaagtt
     4981 gaaacgctaa tcgaaaacat cgaatggaca gtcggtcgaa caggggtagt aacgccaaca
     5041 gctatcatga caccagtccg agtagcagga acgacagtaa gccgtgccag cctgcataac
     5101 ggtgattata ttaagttgaa ggatatccgc ttgaaagata cagttctgat ttataaagcg
     5161 ggagacatca tcccagaagt ttcgcaagtc gtgttagata aacgacctaa agacagcgaa
     5221 gaataccaat tgcctacaca ttgtccagtt tgtggaagtg aacttgtcca tttggatgaa
     5281 gaagtagcct tacggtgcat caatcctaaa tgccctgctc aaatgaaaga aggtttgaac
     5341 cactttgttt caagaaatgc gatgaatatc gatgggttgg ggccgcgcgt actagaacag
     5401 atgtatgata aaaaactagt agcagatgtt gctgatcttt acaaattgac agaagaagag
     5461 ttattgacgt tggacaaaat aaaagaaaaa tcagcaaata atattttgac agcaatcgac
     5521 aacagcaaag ataattcggt cgagcggttg attttcgggt taggaatccg acatgtcgga
     5581 gcgaaagcag caaaaatttt agcagaacat ttcggtgatc tagaaacatt gagtaaaagt
     5641 gactatgagt cgattattgc gcttgatacg atcggtgata tcattgccga cagtgtagtc
     5701 acttatttca gtaacgaaga agtccatgaa ttgatgaatg aattaaaaca agcaggcgtc
     5761 aatttcgaat acaaagggct tcgaaatgcc caattgcaag aagtcgagtc tccttttaaa
     5821 gaaaaaacag tagtccttac tggtaaacta acacgattta ctcgtgaaga agcaaaagag
     5881 acgatcgaaa atcttggcgg gaaagtgact ggtagcgtat cgaaaaaaac ggatatcgtg
     5941 gtagcaggag aagatgctgg cagcaagctg accaaagcac aagaattagg aatcgaagtt
     6001 tggacagaag atcaaatggc agatgcattg gctaaaagtc gctcagtaga agaatgaaaa
     6061 atgagttttt tcagacaaat ttcaataaac aatttcagac acggcactct tgtgttaaaa
     6121 tagagagcaa agtttttaac tcgtttagaa agaaggggta gtatggcgat tagtgaagaa
     6181 caagtgaaac acgttgccaa attagcaaaa cttgcgttcg cagatgaaga acttgcaagt
     6241 tttaccgacc aattaggtaa aatcatcgat atggttgaaa tgctggaaga agtagatacg
     6301 gaaggtgttc catttacgtc gaatgtggtt gaaacagtaa acgtgatgcg acaagatcgg
     6361 gcgcaagctg gatggaaccg cgatgaatta ttgaaaaatg tgccggaaac agaagacggc
     6421 tttatcaaag tacccgcaat catcgataat ggggaggctg gcgcataatg acagaattac
     6481 acaatcgttc tctagaagaa ctgcatcata tgcttgtatc gaaagaaatc actgtacaag
     6541 atttgacaaa aacaacattt gaacgaatca aagaaacaga gcctgaggtc gaggcattca
     6601 ttacattgaa cgaagaaaaa gcattggaac aggcaaaagc tttagatgaa aaagggattg
     6661 atgaaagcaa cgtccttgct ggtattccaa ttggtatcaa ggataatatc gtaacaaaag
     6721 atatcttgac aaccgctgca tctaagatcc tttacaattt tgaaccaatt tatgatgcaa
     6781 cggtcatgga taaaatctat caagcagata tgatccctgt tggtaagcta aacatggatg
     6841 aatttgctat gggtggaagt acagaaactt cttatttcaa gaaaacaaaa aatgcttggg
     6901 acccaacaaa agtacctggt ggctcttctg gtggttctgc agcagcagtt gcagctggac
     6961 aaattccagt ttctttaggt agtgatactg ggggaagtat ccgccaacca gcagctttta
     7021 acggcatcgt agggatgaaa cctacttatg gccgtgtctc acgttttggt ttgattgcct
     7081 ttgcttcatc tcttgaccaa atcggaccaa tgacccgtac ggtgaaagat aatgcattag
     7141 cattgacagc tatcagcggt tatgacgaaa aagacggtac ttcttcaggc gtttcagttc
     7201 ctgattttgc agagggactg acaggcgaca tcaaaggaat gaagattgct ttacctaaag
     7261 aatacttagg cgaaggtgtg gcccctggcg taaaagaagc tgtattgaaa gcagcagaaa
     7321 cattccgttc attaggtgct gttgtggaag aagtcagctt gcctcattca aaatacggtg
     7381 tggctgttta ttatatcatt gcctcatcag aagcaagttc caatttgcaa cgattcgatg
     7441 gtatccgcta cggttatcgc tctgaaaatg tgaaatcatt agatgatgta tatgtcaact
     7501 cacgttcaga aggattcggt gaagaagtaa aacgccgtat catgttaggt acattctcct
     7561 taagcgcggg ttattatgac gcttacttca aaaaagcagg acaagtccgt acattgatca
     7621 aacgagattt tgaaaaagtc tttgcagatt atgacttgat catcgggcca tcttcaccaa
     7681 cagttgcatt cggaatcggt gagaacatta acgatccaat cacaatgtac atgagtgaca
     7741 tcttaacgat ccctgtcaac ttggctggac ttccaggtat gtctgtaccg gcaggtttat
     7801 cagaaggatt accagttgga ttgcagatca tcggtaatta ttttgatgaa aaaacgatgt
     7861 atcaagcggc atatgcgttt gaacaagcaa cagaattcca taaacaacaa ccagctattt
     7921 taggagggaa agacgaatga attttgaaac agtcattgga ctagaagtcc acgtcgagtt
     7981 aaaaaccaac tcaaaaattt tctctccagc gccagctcat tttggtgcag aaccaaacag
     8041 caatacaaac gtgatcgact ggggttatcc aggcgtgctg ccagtaatga ataaagctgc
     8101 gctggaattc gggatgaaag cagctttggc attaaactgt gaaatctcta aagatactca
     8161 ttttgaccgg aaaaattact tttacccaga taatccaaaa gcatatcaaa tttcccaatt
     8221 tgatcaacca atcggtcatg acggatggat cgaaatcgaa gtagaaggta aaaagaaaaa
     8281 aatccggatc gaacgtgtcc atttagaaga agatgctgga aaaaacatgc atggtattgg
     8341 cggttattct tacgttgatt tgaaccgaca aggcacgcct ttgatcgaaa tcgtttctga
     8401 agctgatatg cgttcaccag aagaagcata cgcttatcta gaagcattgc gatcaattat
     8461 ccaatttaca gaagtcagcg atgtgaaaat ggaagaaggc tctatgcgct gtgatgccaa
     8521 tatttctctt cgcccttatg gacaagaaga gttcggtacg aaagcagaat tgaaaaactt
     8581 gaactcttta tctttcgtga aaaaaggttt agcttttgaa gaaaaacgtc aagcaaaagt
     8641 tcttctttca ggaggagaaa tccaacaaga aacacgccgt tttgacgaaa caacgaataa
     8701 aacagttttg atgcgtgtca aagaagggtc tagtgattat cgttacttcc cagagcctga
     8761 tatcccgaaa ttcgtgatcg atgacgaatg gattcaaaaa gtaaaagcta gtttgcctga
     8821 gatgccagct tctcgtcgcg aacgctatat ccgtgagttt ggacttccag agtatgacgc
     8881 aatggtattg acacttacaa aagaaatgtc tgattttttt gaagctgttt tggaaaatgg
     8941 tgcagatgcc aagcaagcat ctaactggtt gatgggtgaa gtttctgctt acttgaacag
     9001 tgagaaactg gaattagctg agacaaaatt gacacctgag aacttagcag gaatgattcg
     9061 tttgatcgcc gatggaacga tcagctcaaa aatcgccaaa aaagtcttcc gtgaattgat
     9121 cacaaatggc ggagaagccc aagaagtagt tgaaaaaaat ggttgggtgc aattatctga
     9181 tccgaacaaa ctactgccaa tcattaatga aattttggat aacaaccaac aatctgtcga
     9241 tgatttcaaa aatggaaaag atcgtgcggt tggcttccta gtcggtcaaa tcatgaaagc
     9301 aactaaagga caagcaaatc ctggtgtcgt gaataagcta cttaaagatg aattagcgaa
     9361 acgatagagg ctgaaaatga aaaaagcacg cgtaatttat aaccccacct cgggaaaaga
     9421 attattgaaa aaaaatttgg cggatatcct acaggcatta gaagaagcag gatttgaagc
     9481 cagcgcctat gcaactacac cagaaccaga ttcagccaag aatgaagcat gtagagtcgc
     9541 agaactaggt tttgatctag tagtggcagc tggtggcgat ggcacaatca acgaagtggt
     9601 taatggaatc gctccgcttg aacaccgtcc taaaatggcc atcatcccag ctggtacaac
     9661 gaacgactat gctcgagcat taaaaattcc tagagataat attaaagcag cagcagaagt
     9721 gataaaaaaa caccaaaccg taaaaatgga catcggcctt tctgataaca gttacttcat
     9781 caatatcgct gcaggtggct atttgacaga attgacttat gaagttccat cagaactaaa
     9841 gagtattttt ggttatcttg cttatcttgc gaaaggagca gagatgctcc ctcgagtaaa
     9901 gccaatcaaa atgcgcttga agtatgatga aggggaatat atcggaaatg cttcgatgtt
     9961 tttcctaggg ctgacgaact ctgttggagg attcgaacag atcgcgccag atgccaaatt
    10021 ggatgatggg aagttttctt taatcattgt gaagacagca aatatctttg agatcttgca
    10081 tattgcagca ctcatgctga atggcggtcg acatgtcgat gatccaagaa taatctatac
    10141 caaaacaagc agtttgttcg ttgaaacaga agatgatatc aatcgaccag taatgatcaa
    10201 cttagatggt gaatacggcg gggaagcacc gatgcatttc caaaatcttc atcaacacat
    10261 tgagttcttt gccaataccg atcagattcc tgatgaagca atcacaggta cagatgaaga
    10321 agaattagag gttgtcagca aagaattcgt aaaagaagtc gaacgtttaa cggatgaaga
    10381 tatcgacggt gacggaaaaa tagcagacga aacacctaat caaaagaatt aatcaaaagc
    10441 cggtgctgaa cgtagtaagg tcagtgccgg ttttagttgt gtaattagct gatttatagt
    10501 agagttcagt aggattgaaa aataggctta aaggagcaaa atatgacaga acagcaaata
    10561 gtaaagaaaa acgaacgata tacagtcgaa atcgtcgact tgagttatga aggaatgggg
    10621 gtagcaaaaa tcgatggtta tccattattt atcgaaaatg cactccctgg agaagaagta
    10681 gaaatactag tagtaaaagc aggcaataag tttggatatg gaaaagtcga aacatttatt
    10741 acacaatcac ctgatcgtca gcctgtagaa aacaatgcat tacttcgcat aggtatcgca
    10801 cctttagccc atttgaagta tgaacaacag cttgtcttca aacaaaaaca agtagagcat
    10861 gtaatggcga agattgctaa gatgccagaa gttcctgtac ttccaacggt cggaatggca
    10921 gaaccattcg gctaccgtaa taaagcacag atccctgttc gccgtatcga tggacagtta
    10981 gccaccggtt tttacaaaaa gaatagccat gatctagtcc ctatcgaaga tttttatatc
    11041 caagatcctg tcatcgatca agcaatcaaa gtagtcagag atatcttgca acggttccaa
    11101 gtacgaggat ataatgaaga aaaaaatgaa ggacagcttc gtcacatcat tatccgtcgt
    11161 ggtcattatt cccatgaaat gatggtcgtt ttagttactc gtaaagaaaa attctttaaa
    11221 tccaaggaaa ttgctgaagc tattcatgaa gaattgccag aagtggtttc ggtcatccaa
    11281 aatatcaatg aagaaaaaac aaatgtgatt ttaggagaca aagaaaaagt tttatatggc
    11341 agagactata tcgaagatca gctattagga aaaacctatc gaatctcagc aaagtcattt
    11401 tatcaagtaa atactcaaca agcagagtat ctgtaccaaa cagccatcga gtttgccgag
    11461 ttagataaaa aagacattgt gatcgacgct tattcaggaa taggtacgat tggcttgtct
    11521 ttagcagaca gagtggataa ggtgtatggg atggaagtta ttccagaagc aattgaagat
    11581 gcacagttca atgctttaaa taataaaatc gaaaatgttc attatgaagt cggaaaagca
    11641 gaaaaagtta tgaaaaaatg gcaagaaaaa gggatcaaac catctgtgat cgtagttgac
    11701 cctccacgaa aaggattaga tcatcatttt attgaatcag caatcgaaat gtcaccagaa
    11761 cgaatcgtct atatctcatg taatccggca acttttgcaa gagatgctaa gttatttgca
    11821 gaagcaggtt atgccgtgaa gaaagttcag cctgtagatc ttttcccgca aacccatcat
    11881 atcgaattag tcgcttcatt cgttaaagga gaataaaaat ggaccaaaaa gaaaaaatga
    11941 aagaattgat caaaaagaaa caaggtggag gccgatccaa acaaatggaa acacctaaga
    12001 atgatcgtaa aaacatgaga aaaggcccta aaatctttaa taaatagacg tgctaaagaa
    12061 agactaagaa gtgttaactt ttgttattgt ttcttagtct tttta
//
LOCUS       DAZULY010000079        11988 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000079 DAZULY010000000
VERSION     DAZULY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.080"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            65..307
                     /gene="yaaA"
                     /locus_tag="ACG81A_001372"
     CDS             65..307
                     /gene="yaaA"
                     /locus_tag="ACG81A_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFS5014743.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            294..1418
                     /gene="recF"
                     /locus_tag="ACG81A_001373"
     CDS             294..1418
                     /gene="recF"
                     /locus_tag="ACG81A_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFS5014744.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1415..3361
                     /gene="gyrB"
                     /locus_tag="ACG81A_001374"
     CDS             1415..3361
                     /gene="gyrB"
                     /locus_tag="ACG81A_001374"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFS5014745.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3381..5852
                     /gene="gyrA"
                     /locus_tag="ACG81A_001375"
     CDS             3381..5852
                     /gene="gyrA"
                     /locus_tag="ACG81A_001375"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFS5014746.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6088..6387
                     /gene="rpsF"
                     /locus_tag="ACG81A_001376"
     CDS             6088..6387
                     /gene="rpsF"
                     /locus_tag="ACG81A_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFS5014747.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6437..6949
                     /gene="ssb"
                     /locus_tag="ACG81A_001377"
     CDS             6437..6949
                     /gene="ssb"
                     /locus_tag="ACG81A_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS5014748.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6975..7211
                     /gene="rpsR"
                     /locus_tag="ACG81A_001378"
     CDS             6975..7211
                     /gene="rpsR"
                     /locus_tag="ACG81A_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFS5014749.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7354..9327
                     /locus_tag="ACG81A_001379"
     CDS             7354..9327
                     /locus_tag="ACG81A_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFS5014750.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9333..9785
                     /gene="rplI"
                     /locus_tag="ACG81A_001380"
     CDS             9333..9785
                     /gene="rplI"
                     /locus_tag="ACG81A_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFS5014751.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10059..11426
                     /gene="dnaB"
                     /locus_tag="ACG81A_001381"
     CDS             10059..11426
                     /gene="dnaB"
                     /locus_tag="ACG81A_001381"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFS5014752.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         4003 a         2178 c         2655 g         3152 t
ORIGIN      
        1 gttttattgc gaaaaaaaag gtataataga gtatactttt aataatgaaa atgagggatg
       61 ggcattgaaa caacaagtca ttctaaatac ggaattcatg acgttgggcc aaatgttgaa
      121 agaagtcaca gttatcggta gtggcggaca agcaaaatgg tatttggcag aaaacactgt
      181 gttagtcgat ggcgaaccag aaaatcgtcg tggacgcaaa ctttatcccg gcatgatggt
      241 tgaagttcca gaagtaggaa ctttttttat ggtaaaaaaa ggaaacgacg ataatgaggc
      301 tgaataagct gtatttaaaa aattatcgaa attacgaaga gctgaacatc gaattttcca
      361 agaacctcgt catcttttta ggagaaaatg cgcaaggaaa aacgaatctt ttggaaagta
      421 tctacgtatt agctatgacg cgaagtcatc ggacaagtag tgagcaggaa ttgattggct
      481 ggactgacga tcaggcgatg attcaaggag agatcacaaa aggttcatcc actcttccat
      541 tagaaatctt attatctaaa aaaggccgaa aaaccaagat caaccatatc gaacagaaaa
      601 agttaagtac ttacgtggga caaatgaatg tcattttatt tgcaccagaa gatctttctt
      661 tagtaaaagg cagcccgcag accaggcgga agtttttaga tatggaatta ggccagatca
      721 atccaatcta tctttatgat cttgtccaat accaagcaat tttgaaacaa agaaaccagt
      781 acttgaagca attgaatgaa aaaaaacaaa ctgatttgct ttatttggat attttgtccg
      841 aacaactagc agcgtttggc agcaaagtat tgaaagcaag agcacagttc gtcaagcgat
      901 tggagttttg ggctaattct ttgcatcagc aaatcaccca tcaaaaagag cagctggaaa
      961 tcgagtatct caccgcagtc gattctttag aaacacatac gcaagagcaa atccaagaac
     1021 aatttctcgc acttttgaac caaaataaaa agaaagacct ttttcgggga acaacgacag
     1081 ttggtcctca tcgtgacgat ctttcctttt ttattaatca gaaaaatgtg cagacttatg
     1141 gatcgcaagg acagcaaaga acaactgcac taagtgtcaa attagcagaa atcgatttga
     1201 ttaaagaaga gacaggggaa tatccaatcc ttttgttaga tgatgtgatg agcgagctgg
     1261 atgataatcg tcaactccat ctgctagaaa caatcgaagg aaaagttcaa acgttcttaa
     1321 caacaacgac acttgaccat gtaaaaaata aaatgactgt tgaacctgag attttttatg
     1381 ttcaacaagg gaaactaaaa ggagaagaaa ctgaatgaca gaagaaagaa gtttagtaga
     1441 acgcgctaaa gagtatgatg ccagtcagat tcaagtactt gaaggacttg aagccgttcg
     1501 taaacgacct ggcatgtata tcggttcgac tagttctgag ggtctgcatc accttgtttg
     1561 ggaaattgtc gataattcga tcgatgaagt attagcaggt tttgcaacga aaatccatgt
     1621 gattattgaa aaagacaaca gtatcaccgt tattgacgac ggtcggggga tacctgtaga
     1681 tatccaggca aaaactggac gtcctgcagt tgaaacggtc tttactgttt tacatgctgg
     1741 aggaaaattc ggcggtggtg ggtacaaagt ttctggtgga cttcacggag taggatcttc
     1801 tgttgttaac gcgctttcta cacagcttga tgtcaaagta tacaaagaag gaaatgtgta
     1861 ctatcaagaa tatcgtcgtg gagctgtagt ggacgactta aagattatcg aacaaacgga
     1921 tcgccatgga acaacggttc actttacgcc agaccctgaa atttttacag agacaactga
     1981 gtttgatttt tcaaaattgg ctacccggat ccgtgaactt gctttcttga accgcgggat
     2041 gcgtatatcg attgaagaca aacgagaaga agaaccagtc atcaatgaat accattatga
     2101 cggcgggatt aaaagttatg tcgaatactt gaatgcaaac aaaaccgtga tttttccaga
     2161 acctgtatat ttggaaggtg aacagcaaga tatcgcagtt gaagtctcta tgcaatatac
     2221 agacggctac cattccaata tcatgagttt tgccaacaat atccatacgt atgaaggcgg
     2281 aacacatgaa tcaggattca agactgcttt aactcgtgtg ataaatgatt atgcccgaaa
     2341 acaaaagctg atgaaagaaa atgacgacaa cttgaccggt gaagacgtgc gagaaggatt
     2401 gacagctgtt atttctatca agcatcctga ccctcaattc gaaggacaaa caaaaacaaa
     2461 attgggcaat tccgaagtac ggacagtcac tgatcggtta tttagcgaac atttcatgaa
     2521 attcttgctg gaaaacccaa gtgtgggccg tcaaattgtt gaaaaaggat tactagcttc
     2581 cagagctcgt ctagcagcga aacgtgcgcg tgaagtaacg agacgtaaag gtgctttaga
     2641 gattagtaat ctgccaggaa aattagcgga ctgctctagc aacgatcctg aaaaatgcga
     2701 attgttcatc gtcgaaggag attcggccgg cggatcagct aagcaaggtc gtaatcgaga
     2761 gtttcaagcc atcttgccta ttcgtgggaa aatcttgaat gttgaaaaag caagcatgga
     2821 caagatcttg gcaaatgaag agatccggtc tttatttact gctatgggta cagggtttgg
     2881 agaagatttt gatgtatcga aagctcgcta ccacaaatta gttatcatga ctgatgccga
     2941 tgtcgacggc gcgcatatcc gtacattgct gctgactctt ttttatcgtt acatgcgtcc
     3001 tattgttgaa gctggatacg tctatattgc tcagccacct ttatacgggg taaaacaagg
     3061 gaagaatatt acgtatgtac aaccaggaaa aaatgcagaa gaagagttga aacaagtcgt
     3121 tgcttcactt cctgcctcac caaaaccaag cgttcagcgt tataaaggac ttggagaaat
     3181 ggatgatcat caactatggg aaacaactat ggacccaagc aatcggatga tggcccgcgt
     3241 cagtgtggac gatgctattg cagcagatca gatatttgaa atgttgatgg gggatcgagt
     3301 agaaccccgt cgtgcattca tcgaggaaaa tgcacactat gtgaagaact tagatattta
     3361 atcatgaagg aggaaaaaga atgagtgaag aaatcagaga aaacatccat gatgtcaatc
     3421 taaccagcga aatgaaagac tccttcatcg attatgcgat gagtgttatt gtcgcacgtg
     3481 cacttcctga tgtcagagat ggtctgaaac ccgttcatcg ccgtatttta tacggcatga
     3541 acgaattagg tgtaacaccg gataaagccc ataaaaaatc tgccagaatc gtcggagatg
     3601 tcatgggtaa ataccatcct catggagact atgcgatcta tgagtcaatg gtacggatgg
     3661 cacagccatt cagctaccgt tatatgctag tggatggtca tggaaacttt ggttctgttg
     3721 acggtgacgg tgccgccgct atgcgttata ccgaagcacg gatgagcaaa atcgctacag
     3781 aaatgctgcg tgatatcaat aagaacactg ttgatttcca aagcaactat gatgatacag
     3841 aaaaagaacc agttgtacta cctgctcgct tcccgaatct tttagtgaac ggaacaacgg
     3901 ggatcgccgt aggtatggcc acgaatatcc cacctcataa cttgtctgag gtagtagcgg
     3961 ctatcgattt gctgatggat aatcctgatg tgacgaccaa tgaattgatg gaagtattac
     4021 ctggaccaga ttttccaaca ggtggtttgg ttatgggaaa atcaggaatc cgaagagcgt
     4081 atgaaacagg taagggttct attaccgtac gtgcaaaagt tgagattact gagatgccaa
     4141 atggtaaaga acggatcatc gtgacagaat tgccttatat ggtcaataaa gcaaaattga
     4201 tcgaacggat ttctgaactg cacagagaca agcgtatcga agggatcaca gatttgcggg
     4261 acgaatcttc ccgtgaaggt atgcggatcg tcattgacat ccgtcgtgac gccagcgcat
     4321 ctgttatctt gaataactta tataaattga catccttgca aaattcattt ggattcaata
     4381 tgttggcgat cgaaaaaggt gtgccgaaag ttttgagctt gaaacaaatt cttgaaaact
     4441 atatcgaaca tcagcgcgaa gtcatcacaa gacggacagt ctttgaaaaa gaaaaagcag
     4501 aagcaagggc acacatttta gaaggtttgc gtattgcttt ggatcatatc gatgaaatca
     4561 ttgcgattat ccgcgggtct aaatcagatg acgaagcaaa agcgacgatg atcgaacggt
     4621 tcgatttatc cgatcgccag tctcaagcta ttttagatat gcgtttacgc cgattgaccg
     4681 gtttggaacg ggaaaaaatc gaaaatgaat accaagaact cttaaaactg attgaagatt
     4741 taactgacat tctggcaaga ccagaacgtg ttacagaaat catcaagaat gaattagcag
     4801 acttgaatca acgatttggt gataaacgac gcacagaatt actagtcggt gaggtcttaa
     4861 gtctggaaga tgaagattta atcgaagaag aagaaatcgt tattacattg acaaacaatg
     4921 gttacatcaa acgtttggca aataatgaat tcagagctca acgaaggggt ggacgtggtg
     4981 tccaaggaat gggtatccac aatgacgact tcgtcaaaaa tctggtttct tgttctaccc
     5041 atgatacatt gttattcttt acgaataacg gaaaagtgta tcgggcaaaa ggatacgaaa
     5101 ttccagaata cgggcgcaca gccaaaggaa taccaattat caatttacta ggtatcgatt
     5161 cgagtgaaag catccaggca atcattgcgg tgacgggaga agcagaagaa ggccactatc
     5221 tcttctttac gacgcgtcaa ggtacagtaa aacgtacttc tgtcactgct tttgctaaca
     5281 tccgaagtaa tggtctgatc gcaattggtt tgaaagaaga cgatgagctc gtcaatgtgc
     5341 tactgacaga tggtcaatct aatatcatta ttggaacaca taacggctac tctgtcactt
     5401 tcaaagaaga agcagtccgc gatatgggac gtacagcttc cggggttaga ggaatacgcc
     5461 tgcgtgagga agattatgtc gttggggcag ccttaatgaa ggaagatcaa gaagtattag
     5521 tgatcacaga aaaaggttac ggaaaacgga caaaagtatc ggaatatcca gtgaaaggcc
     5581 gcggaggtaa agggatcaaa acggcgaaca tcactgaaaa aaatggtcca ctagctggat
     5641 tgaccactgt ttctggagat gaagacatca tgctgattac agataaaggt gtcattatcc
     5701 gtttcaacgt atcgactgtt tctcaaactg gacgttcaac attaggcgta cgtttgatga
     5761 agatggaagc tgatacgaaa gttgtcacga tggcagcagt cgaaccagaa gctgcagaac
     5821 aaacagttga agaacaagga tcagaagaat agccatcaag actaattgtg taagctagca
     5881 agaatgaccg attaaaaaat ctggttattc ctgctggctt ttttgattag ttgaatttct
     5941 cttaaaatat tcgaaactgt tcattctgta ttgcttttga ccaaaataga gtgtataatc
     6001 agagcttgtg agtagtctaa ttaattagat aactctcctt gctcttagga acctaagagc
     6061 cgaaagtcca cagggaggtg aatatcaatg gaaaacacga aatatgaaat catgtacatt
     6121 attcgtccaa acattgatga agaagcaaaa actgctttaa tcgaacgttt cgatgcaatc
     6181 ttgaaagata acggagcaga agttatcgaa tcaaaagatt gggaaaaacg ccgtttcgct
     6241 tatgaaatga acggttaccg tgaaggtatc tatcacatcg ttaaagtatc ttctccatca
     6301 tcagcaaatg ctgtcaatga atttgaccgt cttgctaaaa tcaacgacga tatcattcgt
     6361 cacatgattg ttaaagaaga agcttaattg ttgtttcatg tgaaacaata gaaaacgatg
     6421 agaggagatg actattttga ttaataatgt tgtattagtt ggacgtctga cgaaagatcc
     6481 agatttgcgt tacacagcaa gtggtacggc agtcgcaaca tttactttgg ctgtgaatcg
     6541 taattttaca aaccaaagcg gcaatcgcga ggctgatttt atcaactgtg tgatttggcg
     6601 caaatcagca gaaactttag caaactatgc tcgtaaagga acacttttag gtgttactgg
     6661 acgtatccaa actcgttctt atgataatca gcaaggacaa cgtgtatacg tgacagaagt
     6721 agtagctgac aacttccaat tgctggaaag tcgttctgca tctgaaaatc gccaacaaag
     6781 cggcggattc caaagttctg gtcaatcagc tggcggattt ggcggaaaca ataactctaa
     6841 tcaaacatct caatcatcca acggtatgcc tgatttcgac cgtgatactt ctgacccatt
     6901 cggctcaagt tcaacgatcg acatttcaga cgatgattta ccattctaaa cgaatttgat
     6961 aggagggaaa tagaatggca caacaaagaa gaggcggacg taaacgtcgt aaagtagact
     7021 acattgctgc taaccatatt gaatatatcg attataaaga tatcgaatta ctaaaaagat
     7081 ttatttcaga acgcggtaaa atcttgccac gtcgtgtaac tggtacaggc gctaaaaacc
     7141 aacgtaaaat tactgttgca atcaaacgcg ctcgtatcat gggcttgcta cctttcgtta
     7201 gcgaagacta ataaatccta ggaattgaat gactgatcct tttgatcagc tattcaattc
     7261 ctttttttct gtaaaaataa cctctattcg tttagtcgct cgaatgacga aagttatggt
     7321 aaaatcaaat tagtcatata tgggaggaaa attatggaaa ataaaaacaa tcatttatcc
     7381 ccgaaacctc tcttccttat cctgtttata ttaatcgaag tattggtagt cttaatttta
     7441 ccatttcgtt ttgttagcgc ttcggtcatt ctattgatga atttatgggg tgttcgctat
     7501 tggttgaaaa tgaaacgttg gcttatttta aataaacgtg aaaaaatcca gcaggcttcg
     7561 actgtagctg aaaccaatct agcttatgtc tcagaagtca tgcctgtagg agtgattgct
     7621 tatcagccat caagccaaaa aatcgaatgg ctcaatccat tagcattgaa aatcaaagat
     7681 caaagtggat taacagagga agaattgcta gatacttttt ttactgcaaa agaaaaaaag
     7741 aaaaaccaag tctatctagc agataaaaca tttcagtttg atatggatac agtaaagggt
     7801 gttgtttatt ttaatgatat tacccaaaac cagttgttaa aactccatca gacgaacagt
     7861 cagccggtga ttggtatttt atctattgat aactatgcag atgccattga taaattggat
     7921 gacaaagaga tttcttattt gaactctttc gtcactacac tagtttctga ctggatgaat
     7981 gaataccatg tctttttcaa aagaatcaac gcagaacggt ttttcttcgt agcacagatg
     8041 atcgatctga aaaagatgat ggataaaaaa tttgatttgc tggatcgctt tcgagcagaa
     8101 gctgaaaaac agagctcgcc tatcaccctt agtatgggga ttgcttatgg ctcaagaacc
     8161 ctcgatgaaa tcggtggaga agctcagaat aatttagatg ttgctttagt ccgtggaggc
     8221 gaccaagttg tcgtgaaaga ggtaaaagaa ggagcaaaac ctgtttatta tggggggaaa
     8281 tctgctaaga atgtcaaacg cacgcgtgtt cgttctcgag cgatgagtac cgctttgcgt
     8341 cggatttttg atgaaacagg cgatgtgttt atcatggggc acaagtttcc ggatatggat
     8401 gctttaggct ctgcttttgg tgtcagttgt ctggcacgct tcaaccagaa aaaagcttac
     8461 atcgttatca acgagaacga gatcattcca gatgtagaac ggtgtcttga agaaatccat
     8521 aagcatcctg aattagaaat gcaactaatc tctccagaac gtgccataga gctgaaaaaa
     8581 gaagaggacc tcctcgtgat ggtggattac cataaacctt ctctatcgat ctctcagggc
     8641 ctatatgagc aatttgataa gattgtgatc attgatcacc atcgtcgtgg ggaagagttt
     8701 ccaagtaaac ctctgctgac ttatatcgaa tcttcagctt cttcagcttc agagctagtt
     8761 tctgagctga tccaatacca aagcagcaaa acaaatcgct tagatcgttt tgaggccaca
     8821 ttattgcttg cagggatggt tgtagataca aatagtttca atgtccgtac aactgcacga
     8881 acttttgacg ttgccagcta tttaaggtct tgtggagcag acgcatcctt agtacaatat
     8941 ttattgagct cggatttagc gacatacttg gagatcagca atttgattgc tggaagcgac
     9001 tatgtgactt ctgatattgt tgtggcggca gggacagacg aaaaagagta taatagtgtt
     9061 atagcagcaa aaacagcaga tacattgctt tcgatgatca atatcaatgc agctttcgtc
     9121 attacaaagc ggacagacgg cttgattggg atcagtgcca gaagtaccgg cacgatcaac
     9181 gtccaaatca tcatggaatc cttaggtggc ggaggacact tcacaaacgc agctactcaa
     9241 ctggaaaata cgacagttga ggaaacaaaa cagaagctat ttgctgcaat caaagaaaat
     9301 atgaatcaaa tatatgataa ggagtaatcc aaatgaaagt aattttttta caagatgtaa
     9361 aaggaaaagg taaaaaaggg gacatcaaag aagttccagc aggttatgca caaaattatc
     9421 taatcaaaaa taacttggca aaagaagcaa cgaaaagcag tatcagtcag ttagcaggac
     9481 aaaagaaagc acaagaaaaa cacgaagctg aaatcttgga agaagctaaa aagttaaaag
     9541 aattcttgga aaaagaagat actgtagtcg aattgaaggc gaaagcaggg gaagacagcc
     9601 gtttgtttgg ttcgatccct tcaaaacaaa ttgctgaagg gttaaacaaa caatacaaaa
     9661 tcaaattaga taaacgaaaa atcgaattag aaaatccgat ccgttcacta ggctacacaa
     9721 acgtacctgt taagcttcat catgaggtga cggctacgat caaagttcat gtcgtacaag
     9781 aataacaaat cttgatagcg atgttcagct ggtaagtata accagaagga aaaaagacaa
     9841 accgaaattt atctgagaaa acagctgcag caatgaaagc aaaagctgca aatcgatttc
     9901 agattgattt cggtttcttt tttgggaaca gcaaaatagc ataaagaaaa cgagtataat
     9961 aaaagagaac tattttttta ttacactttc ggtacgaatg atgacttttc tctcattttc
    10021 ttgtaaaatg aagcaattga agacaaggag cgatacgtat gaatgaagtg tggcaggatc
    10081 gaatcccacc gcaaaatatt gaagctgaac aagcggtttt aggctctgtt tttcttgacg
    10141 cagaaacgat cattgatgcc atggagtaca tagagccaaa agacttttat cgtcgtggcc
    10201 atcaaattat ttttgagaca atgatcgagt taaatgatcg taatgaagcg attgacgtgg
    10261 ttactgtcaa agaccggctg gaacaagcca atttgttaga agatgccggt ggtttaagtt
    10321 atctctcaga cttggcatta gcagttccga cagcagcaaa tatcgtctac tatgcgaaaa
    10381 tcgttgaaga aaaatcattg ctgcgtacat tgatccagac agcaacagga atcgttacga
    10441 aaggattcga gcaaggagaa gaagttcaaa caattttaga tgatgccgaa cgcagtatct
    10501 tggaggtttc ggaaaaaaga aatcgaagcg gctttttatc tattgctgaa gtactgaact
    10561 ccacgattga aaatatcgat cgactagctc aaaacaatga agaaatcaca ggtctaccaa
    10621 ctggctatca aggtctggat aaaatgacag caggtttcca gcccgaagaa ctaatcattc
    10681 ttgctgcacg tccagcggta ggtaaaacag cgtttgcatt gaatattgct caaaatgtag
    10741 gaacaaaaac agatcgttct gttgcgatat ttagtttgga aatgggggca gaatctcttg
    10801 taaatcggat gctttgtgca gaaggttcga ttgaggccag ccatttacgt acaggtcagc
    10861 tttcggaaga agaatggtcg aatctgatcg ttgcgatggg gagtctttct cgagcaaata
    10921 tttttatcga tgatacacca gggatcaaaa tctctgaaat ccgtgcgaaa tgtcggaaac
    10981 tagcgcaaga aaaaggcaat ctaggcttga tattgatcga ttatttgcag ttgattgaag
    11041 gtaacggtaa agagaaccgt caacaagaag tatcagatat ctctcgccaa ctgaaaaaac
    11101 tggcgaaaga gctgaaagta cctgtcattg ctctttcaca gttatctcgt ggagtagaac
    11161 agcgacaaga caaacgacca gtgctaagtg atattcgtga atccggatca attgaacaag
    11221 atgcggacat cgtagccttc ttgtatcgag atgattatta tgaaagagaa ggcggagatg
    11281 acgatgatgg aaacgagcca aaaaatgaca acgtcatcga agtcatcatc gagaagaacc
    11341 gaagcggtgc acgaggaaca gttgaactac tgtttatcaa agaatacaat aagttttcgt
    11401 cgctttcgcc aagagaagaa ttttaaagaa gaggttgtga gccttgagca atcccgagag
    11461 ctctcgtaag aagatgcaag gagtaaccgc aggaacgact agagtactta atttaaacag
    11521 atctccaaag ggattgtgac aaaagtcttg tcacagtccc tttttccgaa taaacggtgt
    11581 tcaatcagct gacccttggt attagctcag caaacgtcaa aatatgagga actattttga
    11641 cgtttgtttc tctaatactc aggtcaaaac gctgatctta cgacctcttt tccttaaacg
    11701 gtgttctcct gccaactcct cggtcacaag tcacaatatc atcaaacatg agaagcagtt
    11761 ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacaacct ctttcttaaa
    11821 cggtgttcaa aagcttgcat ctgcggtaat acgcccaatc aaacgaaaaa tatgagaagc
    11881 tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcaca acctctgatt
    11941 cacggtgttc caaaagtagc ttcttcgaaa aataagtcga aatttcac
//
LOCUS       DAZULY010000080        11760 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000080 DAZULY010000000
VERSION     DAZULY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.081"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            148..1164
                     /locus_tag="ACG81A_001382"
     CDS             148..1164
                     /locus_tag="ACG81A_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5014753.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            1305..2606
                     /locus_tag="ACG81A_001383"
     CDS             1305..2606
                     /locus_tag="ACG81A_001383"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFS5014754.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            2652..3314
                     /gene="deoC"
                     /locus_tag="ACG81A_001384"
     CDS             2652..3314
                     /gene="deoC"
                     /locus_tag="ACG81A_001384"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFS5014755.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            3335..3733
                     /locus_tag="ACG81A_001385"
     CDS             3335..3733
                     /locus_tag="ACG81A_001385"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFS5014756.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            3799..4881
                     /locus_tag="ACG81A_001386"
     CDS             3799..4881
                     /locus_tag="ACG81A_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5014757.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            5093..6655
                     /locus_tag="ACG81A_001387"
     CDS             5093..6655
                     /locus_tag="ACG81A_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5014758.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            6648..7781
                     /locus_tag="ACG81A_001388"
     CDS             6648..7781
                     /locus_tag="ACG81A_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5014759.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            7781..8737
                     /locus_tag="ACG81A_001389"
     CDS             7781..8737
                     /locus_tag="ACG81A_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5014760.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            8836..10002
                     /gene="deoB"
                     /locus_tag="ACG81A_001390"
     CDS             8836..10002
                     /gene="deoB"
                     /locus_tag="ACG81A_001390"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFS5014761.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            10026..10844
                     /locus_tag="ACG81A_001391"
     CDS             10026..10844
                     /locus_tag="ACG81A_001391"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS5014762.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            10861..11568
                     /gene="deoD"
                     /locus_tag="ACG81A_001392"
     CDS             10861..11568
                     /gene="deoD"
                     /locus_tag="ACG81A_001392"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS5014763.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         3714 a         2106 c         2736 g         3204 t
ORIGIN      
        1 aataagatac agaacttgta tagaaggctg ccaagtcggt agataaagga ggtttttatc
       61 aaaaaacata ctttcctttt ttctctattc ttttcttggg ggttttggta cactgatagg
      121 gaatttagat agaagggagc caataggatg aagctgacca tcagagatat cgcagaaatg
      181 gcaggcgtat cagtaacaac tgtctcacag attttgaata acaaaggaag cagatttagt
      241 gataagacaa gaaaaaaagt gttgtcgatc gtgaatgaac atcattataa gcccgattat
      301 ttcgcttcaa atgtgatcaa tcgacattca aagaccatcg gaatgatcgt ccctgatgtc
      361 actgatttct tcttctccaa actgatcgaa ggtgtagaga gctatttgaa tcctttgggc
      421 tatgtgatca tgttatgcaa ttctcgtcat agccaagaaa atgagatcaa gtatttacaa
      481 gagctttccc accgctcagt ggacggaatc ttgctggcca caccaaatat tttacctgag
      541 aaatatgcat tagacagcgg tttttatcaa aaaatgccaa tcattttaat cgatcgcgga
      601 ttgaatcgtc gggataacgg acggcttatc gtaaaggaat atgagggcgc ctatcaagct
      661 gtttcgctgt ttattgaaaa tggccatacg aagatcggca tgctgaagga aaccactgga
      721 tactatcaat tagaagaacg tttcaacgga tatcgtcatg cgctgaaaga tcatggcctg
      781 ccttttaatg ggaaatttgt tgaacatgga gagttgactg tccaaggtgg atatactgca
      841 tcaaaaaaat tactcaaaca taaagatatc acggctattt tttgcgggaa tgatgccatg
      901 gcgatcggat gctatcaggc gatcgatgaa ttgggcaaaa aaatcccaga agatatctca
      961 gtcataggat tcgatggatt aaagctgtca gaatacatga ttccgcaatt gacgacagtg
     1021 cagcagccaa gttttgatat tggattttat gctgcacgtt tcctgatcga tacgatcgaa
     1081 tttcctcaaa gaaaagttcc gaacaaagtg ttcgaaacaa aattaattat tcgtgagagc
     1141 gtaaaagtat tgacaaaggg ctaactcggc ggtatattgc ttatgtaagc atttacaaag
     1201 gctagttagt gcttttttta tttttagtaa accagtttac taaagtggtt tacctttcct
     1261 aaaaataatg tataataccg aatgaagaca aggaggatca ggcaatgcga atggtggatc
     1321 tgattgaaaa aaagagggat ggaaacgagc tgtcgaaaga ggaaatcgag tacatcgtca
     1381 cgaactatac gaatggaaag attccagatt atcaagtcag tgcattattg atggcaattt
     1441 tttatcaaga catgacaaat gaagaaatca cgaacttaac attggcgatt gctaattctg
     1501 gtgatgtcat cgatttgagt tctcttgaag gaataaaggt agataagcat tcgacaggtg
     1561 gcgttggtga cacgaccaca ttgattttag cccccctagt agctagtgtc ggagtgacag
     1621 tggctaaaat gtctggacgc ggtctaggtt acacaggtgg tacactagac aagctagaag
     1681 caattcccgg atttcagatc gagctttcgg atgaagaatt tgtacgcatt gttaatgaaa
     1741 gtaaagtagc ggtaattggt caatcaggaa acttggcacc ggcggacaaa aaattgtatg
     1801 ccttgcgtga cgtcacagcg accgttgatt ctcttccgct gattgccagt tcgattatga
     1861 gtaaaaaaat cgcggcagga gcagatgcaa tcgttttgga tgtgaccact ggagacggtg
     1921 ctttcatgaa gaacatcgaa gatgcaagac gtctggcaaa gaccatgacg agcattggga
     1981 aactggctaa ccgagaaaca gtggcggtta tctcagatat gtcagaacct cttggcgaag
     2041 ctatcggaaa tagtctagaa gttgtcgagg caatcgaaac attacaagga aatggtccag
     2101 aagatctagt agaaatgtgc tacgccttag gaagccaaat ggttgttctt gctggcaaag
     2161 ctaagacaac cgatgaagca agaacgttgc ttcaagaagc tctggaatca ggtaaagcgt
     2221 tagcaaaatt caaagaaatg atccaaaatc aagggggaga cccagcgatc gtggaacatc
     2281 cagaacgaat tttgactgca cgctacacaa tggagcttcc tgctaaacaa agcggagttg
     2341 tgtcaaaaat cgtggcaaat gaattaggaa ttgctgcaat gatgctaggt gctgggcgta
     2401 aaaccaaaga agaagatatt gatcacgctg ttggattgaa actgcataaa aaaattggtg
     2461 acactgtgac aaaaggtgag tcactattga cgatctatag taacgacgaa gaaatcactt
     2521 ctgtcattga attattatat aaaaatattg aaattggtga atcggcaatg aagccaacgt
     2581 tgattcatga catcatcaca gaataaagga aaaaagcgga ctttaccgtt ttaaagaaaa
     2641 ggggaaaaga aatggaacta aaccgaatga tcgaccatac tattttgaaa gcagacgcat
     2701 ctaaagaagc tgtgatgcaa attatcgaag aagcaaaaaa ataccatttt tattctgtct
     2761 gcatcaatcc agcatgggtt gcgctagcga aagaacaatt acaaggtgag ccagtagccg
     2821 tatgtacagt aatcgggttt cctctaggag caaacacacc cgagacaaaa gcatttgaaa
     2881 caacagatgc catcaataac ggtgcagatg aagtcgatat ggtcatcaat atcggtgctt
     2941 tgaaatcaaa ggatgatcaa caagtccaaa aagatatcga agcagttgtt gaagcagcaa
     3001 aagatcgtgc gcttgtcaaa gttattattg aaacatcgtt attagataga gaagaaatca
     3061 tccgagcttg cgaaatagca aaagctgcag gcgctgattt tgtcaaaaca tcaactggtt
     3121 tctcaacagg cggagcaaag gtagaggatg tccgtttgat gcgtgaaact gtaggaccag
     3181 aaatgggtgt caaagcttcc ggcggtatcc ataacgaaga agaagcaatg gcaatgatcg
     3241 aagctggtgc tacaagaatc ggaacaagcg caggagtagc aatcgtatct ggttcaactg
     3301 gtgaaggcta ttaatttttt caaaggagaa gctcatggca gaactcaaac aagaatggat
     3361 caacacagct attgaagcgt tagataaagc ctatgttcct tattcccatt ttccggtagg
     3421 cgcttgtctc gtaacggaat caggcaagat ttatcaaggc atcaacattg agaatgcatc
     3481 atttggatta acgaattgtg ccgagcgcac agcttttttc aaagcagtat ctgaaggaga
     3541 aaaaacattt acgcatctag tcgtagcggg gcacaccccc gatcctatct ctccttgtgg
     3601 tgcatgtcga caagtaatgg cagaattctg ttcccctgat atgccggtta cgctagtagg
     3661 agacaatggt gtgacgaagg caacgaccgt tcgtgcactg ctgccttatg catttactga
     3721 aaaagatcta taactcatgt tcattcagtc agctgactgt tgaaaaataa aacaattatt
     3781 ttaggaggct ttacagaaat gaaaaaagca aaattatttg gtttaggtgc tgtcgcacta
     3841 gcagcagggt tatttttagg tgcatgcggc aacaacggct ctactgattc atccggtggc
     3901 aaaagctcat cagacacaac aactgcagca ttgatcacag acacaggtgg tgtagacgac
     3961 cgttcattca accagtcagc ttgggaagga ctagaaaaat gggggaaaga tcaaggactt
     4021 tcacgaggaa atgatggctt ccaatatttc caatcttcaa atgaatcaga ttacatccca
     4081 aatattgacc aagcattgaa tgctggattc aaaacgattt ttggtattgg ttataaacta
     4141 aaaccagcga tccaagaaca agcaacaaac aatacaggta cgaactttgt tattatcgat
     4201 gatgtcatcg atggattaga taacgttgta tctgcaactt tcaaagacaa cgaagcttct
     4261 tatcttgcag gtgttgcagc agcttatacg actgaaacaa acgtagtcgg ctttatcggc
     4321 ggtgtaaaag gtgaagtaat cgatcgtttt gatgctggat tcaaagccgg tgttgatgca
     4381 ggtgcgaaag aattaggaaa agaaatcaaa gtattgaatc aatatgctgg tgacttctct
     4441 gctccagata aaggacgttc aattgctcaa ggtatgtatg cacaaaacgc agatatcatc
     4501 ttccatgctt caggcggtac aggtaacggt gtattccaag aagcaaaatc attgaacgaa
     4561 tcaggcgaca aaaaagtttg ggttatcggt gttgaccgcg accaatcaga cgaaggcgaa
     4621 tacacattaa acggcgaaaa gaaaaacttc acattgacat ctacactgaa agcagttgga
     4681 acagtagtag aagatttggc acaaaaatca gcggacggta aattccctgg cggcgaacac
     4741 actgtttatg gattgaaaga agacggtgtt ggtctaacag aaggacaact ttctgacgaa
     4801 gcgaaaaaag cagttgatga agcaaaagaa aaaatcattt caggtgacgt aaaagttcca
     4861 gaaactcctg aagaaaacta aaaccatttc tatagccttg ttagacacat gtttgacaag
     4921 gctatggttt gattagaaca agaattgttc atttatccaa gctgccttgt gacagtttgg
     4981 ataagggaac aactccctga agtaatgttt gtttcaaaag aagaacgctc actaagcgtg
     5041 aatcttagtc aatcgttgcc ctagatagaa atcgttagga tgtgaaaaaa aggtggttca
     5101 agaaaatatt gtcatagaaa tgcagaatat taccaaagaa tttggaacat tcaaggcaaa
     5161 tgacaacatc aatttacaag taaaggcagg agaaattcat gcgttgcttg gagaaaacgg
     5221 tgctggcaaa tctacattga tgaacgtgct ttccggatta ttagagccga catcagggaa
     5281 aattttgatg cgtgggaaag aagtaaagat cacaagtccg accaaagcca accaattagg
     5341 gattgggatg gtccatcagc actttatgct tgttgatgcc tttactgtaa cagaaaacat
     5401 cgtgttggga agcgagccta gtcgcgcagg gatgcttgac cataaaaaag cacgaaaaga
     5461 gatccaaaaa gtttctgaac agtatggatt gtcagtcaac ccagatgctt acgttcgtga
     5521 tatttcagtt ggaatggaac aacgggtaga aattttaaaa acactttacc gaggagcaga
     5581 tgtactgatt tttgatgagc cgacagctgt attgacccct caagaaattg atgaattaat
     5641 cgtgatcatg aaggaattag tcaaagaagg caagtcgatc attttgatta cgcataagtt
     5701 agatgaaatc aaagcagtag ctgaccgctg tacagttatc cgccgtggaa aaggaatcgg
     5761 tacagtcaac gttaaagacg taacctcaca acaattagcc gatatgatgg ttggaagaac
     5821 ggtttcattc aaaacgatga aaaaagaagc gaagcctcaa gaagtcgttt tgtctattga
     5881 aaatctagtg gtgaaagaaa atcgtggatt agaagccgtg aaaaacttga acttagaggt
     5941 tcgtgctggc gaagtacttg gtatcgctgg aatcgatgga aacgggcagt cggagttgat
     6001 ccaagctttg actggtttgc gaaaggcaga aagcggacat atcaagctaa aaggggaaga
     6061 catcactaat aaaaaacccc gaaaaatcac tgaacatggt gtaggacacg tgccagaaga
     6121 ccgtcataaa tacgggttag tcctagatat gacattatct gaaaatatcg cactacaaac
     6181 ctatcatcaa aaaccttaca gtaaaaacgg catgctgaat tattcagtaa tgaatgaaca
     6241 tgccagagaa ttgatcgaag aatatgatgt tcgaacaacg aatgaacttg ttcccgcaaa
     6301 agctttatca ggcggaaatc agcaaaaagc aattatcgct cgtgaagtcg accgagatcc
     6361 tgatctgttg attgttgcaa atccaactcg tggactggat gtaggtgcga tcgaatttat
     6421 tcataaacgt ctgatcgaac aaagggacaa atacaaagca gtgttactga tcagtttcga
     6481 attagaagaa attttaaatg tttcggatcg tattgctgtt atccatgaag gagaaattgt
     6541 cggtatcgtt gatccgaaag aaacatctga aaatgaatta ggtctattga tggcgggcta
     6601 taccttagaa gaagcacgaa aagagttaga aaagggggct ggcgcacatg aatgatcgca
     6661 gcgaaaaatt ccgtaattta ttagtgcctg tactttcagt tgttttagga ttgatcttag
     6721 gcgcgatatt gatggctgct tttggttttc agcccatcca aggttattct tctatgctag
     6781 atgcagccct tggttcgcaa agagggatag gtgaaacgtt aaggcaagca acgccgttga
     6841 tttttacagc attaggtttt tctgtagcta actcagctgg gtttttcaat attgggcttt
     6901 ctggacaagc actttgcggg tggatcgtca gtgtctgggt ggcacttgcg tttccagatc
     6961 tgccaaaagt tgttttgctt ccgttatgcg tgatcttagg tgccttagct ggtgcgatgg
     7021 cagcggctgt tccaggttta cttcgagcat tttttggaac aagtgaagtg atcgtaacaa
     7081 tcatgatgaa ctatatctta ttatacgtaa gcacatttgc tttacatgaa gtgatgcctg
     7141 aaagctatcg ctcaagtttg gactcttcca atatgatcag tcaaaatgcc tcactgcggt
     7201 taccatggtt aacacaagct tttggcggct ctcgcgtaaa ctcaggatta tttttagcaa
     7261 tcatcgcgct tattttcgtt tggctgctga tgaagaaaac gactttagga tttgaaatcc
     7321 gttctgttgg attgaatcct tttgcttctg agtatgcagg aatgagtagt aaacgtacga
     7381 tcgttttgtc gatggtcatt tctggcgctt tggcagggct tggcggtgtg gttgaaggat
     7441 taggaacgta tcaaaacttc tttgttcaaa cgacttcgct atctattgga tttgacggaa
     7501 tggctgtttc cttgcttggt tctggatcag caatcggtat tcttttatca gcgatcttgt
     7561 tcagtatttt gaagatcggt ggacttggca tgcagacagg agcaggtgtt ccgtttgaga
     7621 tcgtcaatgt atcgattgct ttgattatct tctttgtcgg gatcaacttc ctgattcgtt
     7681 tcgtcatggc taaagtcttc caagggaaaa aacaagaaga gatcgttgcg acaatagaat
     7741 cgaaaccaac gaatcaaaac catgaagggg gcgaactata atgtcgacta tcgaattaat
     7801 tgccttgatc cttacttcta cactggttta ttcaacaccg ctaattctaa cgtcactagg
     7861 cggaactttt tctgaacgaa gcgggatcgt aaacgtaggg ctagagggaa tcatggtcat
     7921 gggtgctttt agttccatcg ttttcaactt gacgatgagt ccatcacttg gttctatgac
     7981 accatggatc gctattttag tgggtggcgt cgtaggtatc ctattttctc tgattcacgc
     8041 agtagcaaca gtcagtttgc gtgctgacca tattatcagc gggacagtaa ttaatctgat
     8101 ggcacctgct ttaggtgttt tcttgatcaa agcatggtat ggtaaaggtc aaactgataa
     8161 catcacgcag aatctggggt atttttcttt cccaggatta gcagatattc cggtcattgg
     8221 accaattttc tttaaaaata cattcttacc tgcttattgt gctattttga ttgctgtcct
     8281 agcttgggtg atcatcttca agacgaaatt cggcttgcgc ctccgttcag ttggtgaaaa
     8341 tcctcaagct gccgacacat taggtatcaa tgtatatgct atgcgttatt caggcgtact
     8401 gatttctggc ttgttaggcg gtatgggcgg tgctgttttt gctcagtcga tttccggtaa
     8461 cttttcagct ggtacgatcg ttggacaagg gttcatctcc atggctgcta tgatctttgg
     8521 taagtggaat cctttagggg caatgggtgc ctcattgttc tttggttttg ctcaaagctt
     8581 gagtatcatt ggcgcacaat taccagttat ttcttcgatt ccgccagtat tgctgcaaat
     8641 tgctccatat ttgctgacaa tcattgtgct ggttgttttc ttagggaaag cttctggtcc
     8701 aaaagcaaac gggaagaact atatcaaatc aaaataatct tttagcgaat aaaaagggtg
     8761 ggttacggga agtgtttttc gtctcgccct ttttgtttta aaggcaataa aaataaaaag
     8821 aaagaaggaa taatcatgtt taaacgcgta catctgatcg tcatggattc agtaggaatt
     8881 ggagaagcac cagatgccga taagttcggt gatgttggtt ctgatacatt aggacacatt
     8941 gcgaaagaag caggattgaa cgttccacat ttagagcagc ttggtttagg aacgatccgt
     9001 cctcttgaag gtgtggaatc agtgaaagat catcaaggct atgcgacgaa attagaagaa
     9061 gtttctgtag ggaaagatac gatgacaggc cactgggaaa tcatgggatt gaatatcaaa
     9121 actccatttc gtgtgtttcc taatggattc cctgatgatc tgatcaaaca aatcgaagaa
     9181 ttttctggcc gtaaagtcgt atgtaacaaa ccttatagcg gaacagctgt tattgacgat
     9241 tacggtgaac accaaatgaa aacaggcgat ttaattgtat atacttctgc cgatcctgtc
     9301 cttcagatcg cggcacatga agaaatcatt cctttagaag aattatatcg gatttgccaa
     9361 ttcgttcgtg atataacaaa agatgatccg tatatgattg gccgaatcat tgcacgtccg
     9421 tacgtgggtg aaccaggaga cttcacacga acaagcaatc gccatgatta tgcgttgaat
     9481 ccattcggac acacagtcct tgattctttg aaagaagcag gaaaggacgt gattgctgtt
     9541 ggaaagatca acgatatttt caacggacaa ggaatcactg aatctgtccg cacgaaaagc
     9601 aatatggatg gcgttgacca gctgctgaaa gttatgaaaa acgatttttc aggaatcagc
     9661 tttaccaatt tagtagattt tgatgcatta tacggtcatc gtcgggatgt agtcggctat
     9721 gcgcatgcga ttgaagaatt tgacttacga attccagaat tgcttgaagc gatggaagaa
     9781 gacgacttgc taatgattac agccgatcac ggaaacgatc cgacgtttac tggtaccgat
     9841 catacgcgag aatatgtacc gttattggtt tacagtaaaa aaatgaaaga acaaggggca
     9901 ttgcctcaag gatattattc agatatttca gcaacgattg ctgagaactt tagtgtttct
     9961 tcgactgaaa atggcgaaag cttcttgaaa ttattaaaat aatcttttta aataggagaa
    10021 agcaaatgac caagttaagc gagatgttac aagaaacgac ccagtttatc cttgataaag
    10081 gagtaaaaga aattgatttt ggtttgattc taggttcagg attaggtgaa ttagcagatg
    10141 aaatcgaaga tgctgtaatg atttcttatg atgagatccc gttctttcct gtttcgacag
    10201 tagttgggca tgcaggaaag ctagtttatg ggtctctggc aggcaagaaa gtgttagcca
    10261 tgcaagggag attccactac tatgaaggtc attccatgca aacagtgacc tatccagtac
    10321 gtgtgatggc cggactaggg gcccattcag tcattgtgac taatgcatgt ggtggtgtca
    10381 acgaatcatt tgtaccagga gatttgatgc tgattaccga tcatatcaat tttactggtg
    10441 aaaatccttt gatcggtccg aatgaagatg caatgggccc tagatttcca gatatgagtc
    10501 aagcttatac gcctgactat caagcaacag caaaagcagc agcagaaaaa ctaggtttgg
    10561 atcttaaaga aggcgtatat atgggctatt ctggaccaac ttatgaaacg cctgcggaga
    10621 tccgaatgtc tcggacaata ggagccgatg cagtagggat gtcgactgtt ccagaagtca
    10681 tcgtagcagc tcacagcgga ttaaaagtgt taggaatctc ttgtattacc aacttagcag
    10741 caggtatgca agcgaatctg agccatgaag aagttgtaga aacaactcag agagtcaaac
    10801 aatcttttaa agcattggtt aaagaaacat tgacattatt gtaataggag gaacaataaa
    10861 atgagtgtgc atattgaagc gaaagaaggc gagatcgccg ataagatttt acttccggga
    10921 gatcctttgc gagctaaata tattgcggag acttttttgg aagatccagt ttgttacaat
    10981 caggtacgcg gtatgttagg ctttaccggc aaatataaag gagaacgtat ttctgttcaa
    11041 ggaacaggaa tgggaatgcc atcagccacc atctatgccc atgaattgat ccagtcgtat
    11101 ggtgtaaaga aattgattcg agtaggtaca tgcggtgcat tgtcaaaaga cgtccatgtt
    11161 cgtgatttag tactagcaca aggtgcagcg accagttctt cgatgatcga aaaaaatttc
    11221 caagcctttc attttcctcc aatcagtgat ttcaatctat tactgaaagc atatgagata
    11281 gctaaagaaa agggctatac ggttcatgta ggaaatgttc tttcggaaga ttctttctac
    11341 aaagatgatt tgacagagac gttccaatta gctgaacttg gcgttttagg agtggaaatg
    11401 gaagcagcgg ctctttatta cttaggcgcg aaataccatg tgcagacatt aagtttgatg
    11461 accgttagcg accatttgat caccggtgaa gaaacaacag ctgcagaacg tcaatctacg
    11521 ttcaatgaaa tgatcgaagt aggattggaa acagcgatag cagaataaga aggaaatgaa
    11581 aaagctgccc tgcattaagt aataagaaca tccaagcaaa aatagctcct catgtttttg
    11641 acttggctgg acttattatt gcaaatgtaa gcttttgaac accgtttacc aagaggttgt
    11701 gaatctgccg tttaactctg agtcacaagg aacaatttca aaaaacagct tttcatgttt
//
LOCUS       DAZULY010000081        11474 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000081 DAZULY010000000
VERSION     DAZULY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.082"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..889
                     /gene="sufC"
                     /locus_tag="ACG81A_001393"
     CDS             116..889
                     /gene="sufC"
                     /locus_tag="ACG81A_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFS5014764.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            902..2188
                     /gene="sufD"
                     /locus_tag="ACG81A_001394"
     CDS             902..2188
                     /gene="sufD"
                     /locus_tag="ACG81A_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFS5014765.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            2185..3420
                     /locus_tag="ACG81A_001395"
     CDS             2185..3420
                     /locus_tag="ACG81A_001395"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFS5014766.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            3407..3877
                     /gene="sufU"
                     /locus_tag="ACG81A_001396"
     CDS             3407..3877
                     /gene="sufU"
                     /locus_tag="ACG81A_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFS5014767.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            3882..5273
                     /gene="sufB"
                     /locus_tag="ACG81A_001397"
     CDS             3882..5273
                     /gene="sufB"
                     /locus_tag="ACG81A_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFS5014768.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(5368..6510)
                     /locus_tag="ACG81A_001398"
     CDS             complement(5368..6510)
                     /locus_tag="ACG81A_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5014769.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(6549..7283)
                     /locus_tag="ACG81A_001399"
     CDS             complement(6549..7283)
                     /locus_tag="ACG81A_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5014770.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            7515..7790
                     /locus_tag="ACG81A_001400"
     CDS             7515..7790
                     /locus_tag="ACG81A_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5014771.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            7888..9885
                     /locus_tag="ACG81A_001401"
     CDS             7888..9885
                     /locus_tag="ACG81A_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014772.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9961..10296)
                     /locus_tag="ACG81A_001402"
     CDS             complement(9961..10296)
                     /locus_tag="ACG81A_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5014773.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            10423..10638
                     /locus_tag="ACG81A_001403"
     CDS             10423..10638
                     /locus_tag="ACG81A_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5014774.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            10675..11037
                     /locus_tag="ACG81A_001404"
     CDS             10675..11037
                     /locus_tag="ACG81A_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFS5014775.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            11083..11409
                     /locus_tag="ACG81A_001405"
     CDS             11083..11409
                     /locus_tag="ACG81A_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014776.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT         3801 a         1950 c         2336 g         3387 t
ORIGIN      
        1 aaaattgttt aataataaat aaaagatatt tgattttagt aaggaaggtc attgcgcttc
       61 attctcaatt agtctaaaat gagtgagaac gaaagagaat ttgggggtat aatgcatgtc
      121 tgtcttagaa ataaaaaatc tacatgtgtc gatcgaggaa aaagaaatcc ttaaaggcgt
      181 aaacctaaca atgaaaaccg gagaaatcca tgccatcatg ggacctaacg gcacaggaaa
      241 atctacctta tctgccgcta tcatgggcaa tccaaactat gaagtaacag aaggcgaaat
      301 tttgtttgac gggaaaaatg ttttagactt ggaagtagat gaacgtgcgc gacttggctt
      361 atttttagct atgcaatatc caagtgagat accaggtatc acgaatgcgg aattcatgcg
      421 tgcagcgatc aatgcgaaac gtgatgaaaa cgacaaaatt tctgttatgg catttttgaa
      481 aaagttagat gaaaaaatgg ctttactaaa tatgccagaa gaaatggctg aacgttatct
      541 gaacgaaggt ttttctggcg gagaaaagaa acggaatgag atcctgcaat tgttgatgtt
      601 agaacctaca tttgcaattt tagacgaaat cgattcaggt ctggatatcg atgctttgaa
      661 agtcgttgct aaaggagtaa atgaaatgcg cggagacaat ttcggtgcat tgatcatcac
      721 ccactatcaa cgattattga actatatcac acctgatgtc gttcacatca tgatggaagg
      781 ccgagtagtc atgacaggga atgctgacct agctaaacgt ctggaagcag aaggctacgc
      841 tggtatcagt aaagaattag gaatcgacta taaagaagaa gaagcgtaag ggggagcaag
      901 aatgaaagaa aaaaactggc tcgattatct tgacgcggta aatgattttt ctttaagtaa
      961 aggcgaacct gattggatga gaaccttccg tcaagacgca ctagcgaaag cagatgaact
     1021 gccattgccg catatcgatc gggtgaaatt ccatcgctgg tcgttatttg atgtgaaaga
     1081 aacacaaacc atcagtgaaa caggcacgat tccggcattt gatgcaatga aagacaatcc
     1141 tgtgcttgtc caacaaggtt catggacgat ttttgaacaa ctgcctgtgg aattagcaga
     1201 aaaaggagtc atttttaccg acttgttcac tgcaatgatc gaatatccag aactagtcca
     1261 agaatattat atgaaaaaag cagtaaacat gaacgaagac caattgactg cactccatgt
     1321 agcttttatg aacagcggta ttttcctgta tgtgcctaaa aatgtcgtga tcgatgaacc
     1381 actcgagtcc ttattcattc aagatggcgc atccgatgag cacttcttca agcatgtgtt
     1441 gatcgtggca gatgaacata gtgaattttc ttatctggaa cgtttccaga caaccaaaga
     1501 acaagtagca aaatcatcag gtaatatcat cgtggaagtc atcgctaaag ctggttcaaa
     1561 aatcaaatat tcagccgtag atcaattagg agaaaatatt acttcttata tgaatcgccg
     1621 tgggcacatt ttacgagatg cttctgtcga ctgggcaatt ggtgtcatga atgacggtca
     1681 tgtgatcgct gactttgact cagatttagc aggagaaggt gcacacgcag aagtcaaaat
     1741 cgtggcaatc agctcgggca gacaaatcca aggaatcgat acacgagtaa ccaataaagc
     1801 ccctcatacg atcgggcata ttttgcaaca cggagtgatc cgagaaaaag gaacattgac
     1861 ctttaatggt atcggccaca tcttgaaagg ggcaaaaggt gcagatgccc aacaagaaag
     1921 tcgtgtcttg atgctttctg ataaagcacg tggtgatgcg aatcctattc ttttgatcga
     1981 tgaaaacgaa gtaactgctg gacatgctgc cagtgtagga cgagttgatc cagaagaaat
     2041 gtactatctg atgagtagag gattgcataa agaagaagca gagcgcttag tgattcgagg
     2101 attcctcggt tctgtcttaa cagcaatccc tgtagaacaa gttcggaaag aattagtcga
     2161 agtaatcgaa gggaagttga atggatgatc gatgctaaaa agctacgcaa tgattttcct
     2221 atcttatttc aagacgtcaa tgatgaacct ttagtttatc tggataatgc agcgacgacc
     2281 caaaagccaa aacaagtact ccaggcatta gcgtactatt acgaacacga taacgccaat
     2341 gttcatcgcg gtgtccatac gttagcagaa cgcgcgaccc atgattatga acaagcaaga
     2401 gaaaaagtac gggcgttcat ccatgcaaaa gaaacagcag aagtgctgtt tactcgtggt
     2461 acgacgacaa gcttgaattg ggtagccaga accttcggcg accgctatat caaagcagga
     2521 gacgagatag tgctctctta catggaacat cattctaatg ttatcccgtg gcagcagttg
     2581 gcaaaacgaa caggtgctgt cttgaaatat atcgaactaa cagaagaagg cttcctggat
     2641 atggaggatg cagcgaaaaa gattacggac aaaaccaaaa tcgtctccat tgctcatgta
     2701 tccaatgtct taggtgtctt caatcctgtt caagaattag ctgaacttgt ccatgaaaac
     2761 ggcggggtca tcgtagtaga tggtgctcaa tctgccccgc acatggccat tgatgtccaa
     2821 gcactagact gtgatttctt tgctttcagc ggacacaaaa tgtgcggacc aacaggtatt
     2881 ggtgttttat atggtaaacg aaaatggtta gaagaaatgg aacctgttga atttggtggc
     2941 gaaatgatcg attttgtcga attatacgac agtacatgga aagaactacc ttggaaattc
     3001 gaagcaggga caccaaatat cgccggcgct atcgcattag gccatgcaat cgattatttg
     3061 caaaaactcg gtatggacaa tatccatcga tatgaagaag aactagccgc ttatgtgttg
     3121 ccaaaattac aagcaattga tggattgact gtttatggtc cacaagaccc gaattttcgg
     3181 acaggagtcg ttgcattcaa tttggatggg ttacatcccc atgatgtcgc gacagcttta
     3241 gacatggaag gggtagccgt cagagcaggt catcattgtg cgcaaccgct attgaagtac
     3301 cttgaagtat cagcgactgc tcgcgcgagc ttttatttct acaacacaac gcaagacgcc
     3361 gaccgtttag ttgaagcaat catggcaaca aaggagtttt tccaacatgg gactatctaa
     3421 attagacaat ttataccgtc aagtgatctt ggatcattct tcccatccgc atcatcgtgg
     3481 aagcttgact gactcgacaa gcaagatcga attgaacaac cccacatgtg gagatgtgat
     3541 cgaattgcaa gtcgatgtaa aagacggaat catcaaaaat atcgcctttg acggcagtgg
     3601 ctgctccatc agtacggcaa gtgcctcgat gatgacagac gccgtcatcg gaaaatcaat
     3661 agaagaagca gaacatttaa ctgtagactt tctacagctt gtccaaggaa aagaagtcaa
     3721 agatcccgac caattaggcg atgctgctat gcttggtggt gtagctaaat ttcccgctcg
     3781 gatcaaatgt gcgacacttg cctggaaagc actaggaaaa gcaatggaaa acgatggtac
     3841 cgcagctttc catcattacc acaaggaaga ggagtgagaa catgggcgta ccagaattag
     3901 aagaatataa attcgggttt cacgatgatg tcgaaccggt tttcagtaca ggagaaggct
     3961 tgacagaaga agtgatccgt gagatgtcaa gaatcaaagg cgaaccagaa tggatgcttg
     4021 aattccgtct gaaatctcta gaaacattta acaaaatgcc aatgcaaaca tggggccccg
     4081 acctttcaga catcgatttc gatgccatca aatattacca aaaaccaagt gacaaacccg
     4141 ctcgtgactg ggaagacgta ccagaaaaaa tcaaagatac atttgaaaag attggtatac
     4201 cagaagctga acgagcttat ctagccggtg cttccgcaca atatgaatca gaagtcgtgt
     4261 accataatat gaaagatgaa ttcgaaaagt taggaatcgt ctttactgac actgactctg
     4321 ctttgaaaga ataccctgaa ttattcaaag agtacttttc aaaacttgta ccgccaaccg
     4381 acaacaaatt agcagcgctg aattcagccg tatggtcagg tgggaccttt atttacgtgc
     4441 caaaaggcgt caaagtcgat gttccattac aaacgtactt ccggatcaat gcggaaaaca
     4501 cggggcaatt cgaacgtacg ctgatcattg ttgacgaagg agcaagcatc cactatgtcg
     4561 aaggttgtac ggcacctact tattcaagta acagcctgca tgccgccatc gtagaaatct
     4621 ttacaagaaa agatgcgtat tgccgttaca caacgatcca aaactggtca gacaatgtgt
     4681 acaacttagt aaccaaacga gcaaaagcat acgaaggcgc aaccgttgaa tggatcgacg
     4741 gaaacctagg tgcaaaaaca acgatgaaat acccaagtgt ttacttagat ggaaaaggtg
     4801 cacgaggcac catgctctcc atcgcctttg caggagcaaa ccaaatccaa gatacgggtg
     4861 caaaaatgat ccacaatgca ccaaatacat ctagctctat cgtctctaaa tcaatttcaa
     4921 aagacggcgg agaagtaaac tatcgaggac aagtaacctt tgccaaaaac agtgcgggtt
     4981 cgatctctca tatcgaatgt gacacgatca tcatggacga atggtcgaaa tccgatacga
     5041 tcccattcaa tgagatccac aacagccaag tctctttaga acacgaagcc aaagtctcaa
     5101 aaatctcaga agaacaactc tactatctga tgagccgagg cctgaccgaa tctgaagcaa
     5161 ctgaaatgat cgtcatgggc tttgttgaac cattcaccaa agaacttcca atggaatatg
     5221 cagtcgaact caatcgattg atcagttatg agatggaagg cagtgttggg taaacctgtt
     5281 ataggagcta gaacccttga tatataaggg ttctagcttt tttgttttgc ctgtatacta
     5341 gaaatgacca gattttgacc agctctttta ctgatgagcc atcatatcag caaagtcttg
     5401 acctgttttc ttaggtgcat tatttgtaat atgtgcatag atgtctaatg tcactttact
     5461 attttcatgc cctaatcgtt gttgaactac cttgacagat tctcctgctt ctaataataa
     5521 actagcatgt gtatgtctaa acccatgagg tgtaatacga ggcaagttat attttttcaa
     5581 aatatatctt aaccaatcat taacagtgtt tggaatgtat agagtgtttt taactgttgt
     5641 aaaaacatgt tgctctttag agctagtatt gtatccaagt tttaaatact cttctttttg
     5701 ctgtaatttc cagttgttga ggatagaaag agtctcagta tctaaagaga taactcgtct
     5761 agaagctctt gttttcggag attgaattat aactttacca aattcatcta cagcaagagt
     5821 tttattgaca tgtagttctt tattaaacgt gtctatgtct ttccactgta aagcgagtac
     5881 ttcactcttt ctcatacctg tgtatgccaa taatctgaag aaggcgtatt gtttcatgtt
     5941 accaaaatct ttaaaagcat ccagtaaatt attaagctcc tcttttgagt aaaatttacg
     6001 atttttctca tactcttttt ttcgtggaag aagtgttttt tccataggat tattgtcaat
     6061 atattgcatt gcaaccccgt agcgtaaaat ttgtgcagtc tgttttctca tataatcgta
     6121 actttcatat ttactgtgcc attcatctac cactttttga caatatggga cagagatatt
     6181 cgatagttta agcttaccaa atgctggaag tatttgatta tttgcgtatc ttactgagat
     6241 tgcgattgtt gaagctttaa cactggtacg ctgatgagtt atccattcat tatacaggtc
     6301 ttcaaatgtc atatcaggtg ctttcttttt aacaccgtat aatatttgat gtctgtactc
     6361 gtcataatct ttttgagctt cttgtttgct actaaaacct cttctcgtag tatttatttt
     6421 ttcaccagtt cgtggatcgg tggcaatgtg tcctttatac atccacgcca cttgaccgtt
     6481 tttctttttg tactttgata tttttgccat taaatcatct cctcttagtt tacaaggagt
     6541 acttaccact agatggtatc acctcccttc atttttttct tagtgtattt tttcccaatt
     6601 gatgaaatcg tttgttctag cttgtttata ttatcagtaa tttctctcac agcttcttcg
     6661 ggataagaca tgtctttcaa ttgaataggt agccattcat ctaccattct ttctagtagt
     6721 tcttctatac tgctagtgag gaaaagaatg ttattgtcaa attcttctac aatagcattg
     6781 atgaataatt cttctatttt taagatattg aaaagactaa ttttttcatt tttaacttta
     6841 ttacaagtct tgttagcaat aaattcttgc atctctggtt ctaatttgga atatctatcc
     6901 agccatgtat ctaaattaga gtgtactggt gggaactgtt gacgaagatg ctggaacagg
     6961 tttttatctg gaaatgttat accatctagt acaatgctat ctctataaag aataattccc
     7021 aaaatatatt cttctacatt gccacaaact aactcctcta tactcatatt tcccaaatca
     7081 gcaattcggg ataatcgctc tggtattggt aaataggttc ccttctccca ttcgtagata
     7141 gtttttttgt caattaattg accgttgtta atcataagac caaatgctgg ttgtgttaat
     7201 tttttactct ttcttatagc ttttattttt gttccgagta tcttttttcg ttctaaataa
     7261 tttattggtg gacgttttat catattgacc acacctccta aaattagtat acctcaaaaa
     7321 aggggtgttg caaataaatt ttacaaagct gttgacaccc cttttttatc gaggtattat
     7381 gtgtatgtcg atgaactggt aatcattttt tgtttgatta atcggttaat aacaaaatac
     7441 ttttattttg agcaaaaaat acgaagataa ttcttagaaa atttgttcaa gcaatcacga
     7501 aaggaggggg aaatatgtct ctacaaatca ttgatagcat tgtttctata caagttgaac
     7561 aacaagtgaa agagataatt acagtgctaa agcctatgct agaagacatt atttctggag
     7621 tcactctaga gtatatggac tatcagcagg caagtgaata tctaggtgta tcagtaggaa
     7681 cgattagaaa atatgtttct caatacgggt tacctgttat aaaaattgat acggtcatta
     7741 gatttaaaaa atctgatatt gatagtttca tggaacggta taaaggttga gtaaacaaat
     7801 aagactaaaa catatagtaa taaagagttt gtttttatta tctgtatgtt ttagtcttta
     7861 acagaatttt ataaggagga aacacaaatg gaaacatcaa tgattaatat tccaatttat
     7921 gtagatcaag aaaaattatt agagaagcca gttacaaaaa aagaaaaaac tgatgtaaga
     7981 gataggtgtt tagataacta tagagaagtt acaagagatg agtttgtaga gatactaaat
     8041 tcatcacaat catttattcc aagcaagaca aagaataaag gcaatgacaa ggagagtttt
     8101 gtggaaacca gagtaattat tctggatgtc gataacactg ttaaagatga gaaggggaag
     8161 gtagtagatt tgagtaaaga tgatagtaga tacttaagcg tagaaaaggt tttatctatc
     8221 aatttagttc ataattctgc gtttgcgata caaaaaagta ttcggtactc tgaaaatcta
     8281 gaaaaataca agattgtctt tctattaaaa gaagcaatta ctgactatgg taaaatggtt
     8341 gctgtttacg aatacttgaa aaagaacatc cccgggtgtg atgacaatgt aaatgcttcg
     8401 aatagaatgt tttttggagg atataagtca gatgcagtga taataattaa tgaagacaac
     8461 atgttagata ttactgagct tccgattgat tttaccatga catgttctaa aaattgtcaa
     8521 agtgattcag ggagagagta tgaactagtc aatgaaaccg attttgttaa gttgataaaa
     8581 aatgacgaca aagaagaaat gaaacagtgg tttaaagact gcctgtttga ttcctccgac
     8641 atgtctttta atgagattta tgaaagattg ttaaccattg atatgaataa attacttaag
     8701 tcaagtaaaa acttaagatg tctgtttcac aacgatcata atccctctgc tagtattttt
     8761 accagtaaaa ctgggcattc cattttttat tgtcattcca gtaattgtga tatcagtgaa
     8821 aattttattg gagttgtgat gctaatttta aagaaagaca gtcgggtaga aacatttaat
     8881 tggctattag agaacctaga cttgtatcct acacattttc gaaaattgaa agaggaatca
     8941 aaagtacttt ttgacactct tgaatcaatg aaagacaaac ttttcaggac aataaggaat
     9001 tatctagagg aaatgcgacg tgtctatgat gtcttgcttt ctgatgttaa tttttttgat
     9061 gatagtaagg aaagtgtgac ttgtattttg tcgggggaac aattagctaa aaaaatgagt
     9121 tcttactatg gtaaaactta tgacatagat aaatgtaata agttactatc acttatgact
     9181 tttataggat tgataaaaaa attagatgat gaagatgttc ctttgaagat gctcgaatac
     9241 ctaaataggt taaaggaaga gaaagcgtta ggaaacttta atcataagag aagcaatgtc
     9301 tatcgattag acgtttttaa tgcaacttct gtgattcaaa aattgaatga agatatcctt
     9361 ccacttctgg aaaaaattca ttttacatat caatattttt cttatgaatg ggttaagata
     9421 tgttttaacg aaaaagaggc taaaagagta tttcctcaaa attcccgatc agaactcagc
     9481 aaggaaaaac aattgatttt agatgaagca catgaagttt tacagggctt atcgtggcaa
     9541 gggacatatc taatgacaga gaaagaactt attaacactg tttgtgaacg cctacgtaat
     9601 gttggggata acagcgtgaa aaatgcttta ataaaaaaca gaggctactt tgttaaacaa
     9661 ggattcactc ttgaacgagc ttctaaggat attaagaaac tatacaatgt gaaagcaagt
     9721 agtagttttt taatatattt agcgaaaggg gcagggatag atgaaacaat aaaagaattg
     9781 atattatcag gagctgggat agttcctctg tatcaattta caagtgtaac aagagatgat
     9841 ggacggagag taaggaagag aaaagaccca attgtattaa actagacagg ttatccatag
     9901 ctcttaatct tatttcatct caatacaccc accgttaaag ttcggtgggt tatttatttt
     9961 tcaatctttc aatagctgat tgcgttcatt ttcttgcctt tttaatagct tcatatagtc
    10021 aattgttcga actgcatctt cagtgctaag agaacgatag agagccaaaa actcttcctc
    10081 ataatctacg acaacattgt ctagaaaatg gcttacaggg gttcctaggg ctctagaaag
    10141 agctgttaag ctatcgaggt taggctctgt tacagaattt tcccatcttg ctactgcctg
    10201 ttgtgtagag aagatttttt cagctaattc tttttgtgtc cacccgagag cttttcgctt
    10261 ttctctaatg acctttgcat attgtttctg tttcattaat acacccccat ctcagtatag
    10321 tacaacaagt tgttgtattt gtaatggtat ttgaagtttt ttatatttta tgagttgaaa
    10381 tacatcatat tgttgtatat aataaaacag gaggggagaa tcatgaaaaa agtaagtaat
    10441 ctcagccgat acagaaaaga aaaaggactt tctcaaacgg agttagcaaa aaaaatgaat
    10501 gttacacagc aatgtgtaag ttcgtggcaa acagggagaa cgattcctaa accctatcaa
    10561 atgaagatgc tttctgagat attaagtgta ccaatgaatg agctgttttc tgatgtattt
    10621 aataaagtaa acagttaact aataagcaat tgaatgattt taggaggaga agacatgtat
    10681 tgtagaaaat gtggcaaaga aataacagat gaaagtaagt attgccagtt ttgtggagca
    10741 ctacaagaaa cagatgagac aaccggaaca gacacagtaa agaaaagtac tgagcagaca
    10801 attaatgaaa tgaaggaaca aatcgtacat ggacaagaga cagggaagct gtatttgatt
    10861 attggttgga tttctatggt agtatcgtta gcatttattc ctgttgtatt tggtgctgtt
    10921 gctgttatta tgggctactt atatcgagaa aaagatgaga aaatgggtac gattttgatg
    10981 atagcagggg tagctggagc aatctttggt gtacttttag ggatgtcaat gtactgataa
    11041 gagggataag agcaaattat tttggagata ggagataaaa agttgaataa agtaaatatg
    11101 aacaagaagt ttgtcattat aggtattgtt gtcttggcta ttataggtat tattttttca
    11161 aaaatgacag gtacatctgg attagagaag tcatcaaaac cattggtaac acagatttta
    11221 caagaacagt acaacttaaa tcgatcatgt gatgatgtga cgattactca tgaaaatgga
    11281 gataacacat accgggctaa agctatatta gacaatggaa gtgctatcaa cataaatatt
    11341 gagtactatc caaagaaaga ccgtatatat gtggagatac catatgctga ggtgttaatg
    11401 ttaaattgat ttaaatgtat tatgcatacc gtccaaacag gaactcccaa aggtgtaaat
    11461 tcactaggga gttt
//
LOCUS       DAZULY010000082        11437 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000082 DAZULY010000000
VERSION     DAZULY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.083"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..2114)
                     /gene="glyS"
                     /locus_tag="ACG81A_001406"
     CDS             complement(39..2114)
                     /gene="glyS"
                     /locus_tag="ACG81A_001406"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFS5014777.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2116..3033)
                     /gene="glyQ"
                     /locus_tag="ACG81A_001407"
     CDS             complement(2116..3033)
                     /gene="glyQ"
                     /locus_tag="ACG81A_001407"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFS5014778.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3417..4229)
                     /gene="recO"
                     /locus_tag="ACG81A_001408"
     CDS             complement(3417..4229)
                     /gene="recO"
                     /locus_tag="ACG81A_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFS5014779.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4372..5271)
                     /gene="era"
                     /locus_tag="ACG81A_001409"
     CDS             complement(4372..5271)
                     /gene="era"
                     /locus_tag="ACG81A_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFS5014780.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5286..5681)
                     /locus_tag="ACG81A_001410"
     CDS             complement(5286..5681)
                     /locus_tag="ACG81A_001410"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5014781.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5665..6141)
                     /gene="ybeY"
                     /locus_tag="ACG81A_001411"
     CDS             complement(5665..6141)
                     /gene="ybeY"
                     /locus_tag="ACG81A_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFS5014782.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6158..8350)
                     /locus_tag="ACG81A_001412"
     CDS             complement(6158..8350)
                     /locus_tag="ACG81A_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFS5014783.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8374..9345)
                     /locus_tag="ACG81A_001413"
     CDS             complement(8374..9345)
                     /locus_tag="ACG81A_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFS5014784.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10051..11430
                     /gene="brnQ"
                     /locus_tag="ACG81A_001414"
     CDS             10051..11430
                     /gene="brnQ"
                     /locus_tag="ACG81A_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS5014785.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
BASE COUNT         3069 a         2430 c         2096 g         3842 t
ORIGIN      
        1 ttttgtgcct attctctggt ttatttacat attagaaatt attttgtgat gatttgatct
       61 aaacttgcta gcgacaaagc catggcagca atttgactta attgcgttaa tcggttctta
      121 cgtactgctg gttcttctgc catgaccatc gtctgatcaa aataattttc gatcagtggg
      181 cgaaggctta caagctgatc ataattttcg ttgatcgttt ggtcagcaaa gctttttttc
      241 attgcctcta ctgctttgtg cagttctttc tccgcttcat tttcgaacag ttcagggtct
      301 acttctgatt gtttttcaag ctccgctttt ttcgctaagt tcacgacacg tgtcaaagct
      361 tccatcgatg gtttgaagtc ttcatctgca agatgctgct tgaacaattg tgctgctgca
      421 aatacttttg tcagatcttt ttgttctgca ttcaaaacag cttcgatgac atcatgacga
      481 atattcttcg ttgtcaataa ttggcgcaga cggcctttga taaagtcgat cacctgttgt
      541 tgcccttctg ataaaccaat gccgaaacga tccgtatctt tactgataac atcactaatc
      601 gtttcttgca gaacagacaa aggaaaggcc cattttttcg attcgatgat ccgaacgaca
      661 ccgtatgctt ggcgacgtaa ggcataagga tcgtttgacc cgcttggaat catcccaact
      721 gcaaagaaag aaaaaatgct atctaattta tctgcaatcg ctaaaacaga acctactgta
      781 gactgaggta gctctccttc actggatata ggcatgtaat gctcacgaat cgctgttgct
      841 acagcaggtt tctcgccttt cagcagagca tatttttcgc ccatgatccc ttgtagttca
      901 ggaaattccc ctaccatgtt ggttactaga tcaaatttat agatttcagc agctcgttgc
      961 agatccatca attcttcctc agatagtcct acttgttcgc caatgatacg ggcaatgact
     1021 tgtacccgct gcattttctc atagatacta ccgatttttt catggaaagt aacagttttt
     1081 agtttttcaa cgtattggtc gattgaagct ttcttgtctt cattataaaa gaattccgca
     1141 tcttctaaac gagccgtcaa tactttttga ttgcctcgaa ccacattttc caattttacc
     1201 cgatcaccat tacgagcaga aataaaatga ggaagcagca taccttgatc gttccgtacc
     1261 tcgaaataac gttgatgttc tttcatagaa gtgactagta cttcttcagg aacagaaaga
     1321 tatttttctt cgaagtcgcc agaaaagaca gttggatatt cgactaaatt gttgacttct
     1381 tctagtaaat cttcgtctag atcaataacc cagtgatttt tttcggctaa ttcatttgct
     1441 tgtgtaacga tcatttcttg tcttttttgc ggtgaagcaa taacgaattg ttcattcaat
     1501 ttttcttcat attcttttgc atgcgcaaat gtcacatctt cgcctaagaa acgatgtcct
     1561 cgagaggctc tgcctgtttt gacatctaag atctcaaacg ggattacttc gtcatctaac
     1621 aatgccacga tccagtgaat agggcggata tattcaaaat cgtattctgc ccaatgcatc
     1681 gttactggga aagtgaggct tttaatgaca tctgctaaac ctgtcaatac ttctttcgct
     1741 tctttgccgt gagtgtattt cgttacatag acatattcca cgccttttaa ttctctaaaa
     1801 gtgatagcat ctgtcgtcag accttgtccg cggacaaatc cttcagctgc tttcgtccag
     1861 tttccatctg cgtccaaagc aatttttttg gctggtcctt tgacttcttc ttcactgtct
     1921 gcttgacgtt ctggaatgtt tgttactttg attgctaagc ggcgcggtgt agaaaacgtt
     1981 tcgatcgtat cataagtcaa ttggttttca tctaaaaatt ttgctgtttt ttcttctaat
     2041 tgtttcatgc ttggtgtgac aacatgagca ggcatttctt ccagcccgat ttcaagtaat
     2101 aagtcttttg ccatcttatt cagcctcctt catgttttct ggatttgctg tgtttttatt
     2161 caataaaggg aatcctaatt tttcgcgttc tgccacaaag attttggcta cagcacgagc
     2221 catgttgcgg atacgggaaa gataacctgc tcgttctgtt acggatactg caccgcgagc
     2281 atctaataaa ttgaatgtat ggctgcattt caaaatataa tcatacgctg gatgaacaag
     2341 attttcatcg atacagcgtt tagcttcttt ttcaaaacgg tcaaagttgc ttagtagcaa
     2401 gtcttggtca cttacttcaa aagaatattt ggaatgttca tattctggtt gtttgaagat
     2461 ctcgccatat ttcacaccat cagcccattc cagatcatag acactttcaa cttcttgaat
     2521 atatgaagca agacgttcta gtccataagt gatctcagaa gtaactggtt tacaaggtag
     2581 tcctccgact tgctggaagt acgtgaattg tgtgatctcc atcccatcaa gccatacttc
     2641 ccaacccaca ccggcacacc ccatcgatgg gttctcccag ttgtcttcaa caaaacgaat
     2701 atcgtgttct aatggatcaa ttcctaaaag acgcaagctt tctaaataaa gctcttggat
     2761 attttcaggt gaaggtttca ttacgacttg aaattgatga tgctgataca aacggtttgg
     2821 gttttctcca taacgcccgt cagctggtcg gcgggatggc tctacataag cagcattcca
     2881 tggttccggt ccgattgccc gtaagaatgt atatgggctc atcgtacctg ctcctttttc
     2941 tgtgtcataa gcttgcatca gcatgcagcc gtttgatgac caaaattttt gtaatgttaa
     3001 aatcatatct tggacggtca attttttact catctgtttt gcccctttca atttttaagc
     3061 gttgctgtca ataagaatac aggaaagaaa acgacaaaaa gtccctatgt ctgattacag
     3121 acatagggac gaataatttc gcggttccac cctacttccg aataaagatt cggcagcttc
     3181 atttatcgta ctcgcaagtg cctcttcaaa aatcgtttgg gtccggcttc cactctcccg
     3241 aactcgctaa accacgtgtt ttttactgat cttggtcaat gtacgtattc attttgcagt
     3301 tttcttgttt atataataga tcctctcagt gaacgatcta tttaatctac ggttaataat
     3361 agacggaagt ccctgtattt gtcaaatagt tttgattttt ttgttgtatc tttcatttat
     3421 tcttcttttt caggtatctc tttttcaggc atatttttct ctggcgtctt taatacgtgt
     3481 gcccaagact tcatttgatc aatgaacttt ttgcttttta gatgaatacc gacatattca
     3541 tcatacaact ggtcaatgag gtcacggatt gcctgcttcg tttcttcttt caagtggatt
     3601 ccactgatct tatcataaga aatagccgaa aacattcgaa cgaaataaac agcgcgagga
     3661 tctgcatgat aacggtgttc gtccattggc cagtgtttct cgcatatcac accgccagat
     3721 ttggaagaat agtcaaattt tccttttgtt tccccgcata cgacacagtg cgtccaaatt
     3781 ggcgcgattc cgaagcgttg cagtatttga acttcaaaga tattcgtgat gatttctgca
     3841 tcatctcctt gatccatccg ctgcagagct tgttgcgtaa attggaataa atgcgggtca
     3901 tattgatgat cttcgattgc tgcatctact agatttagca aataggtacc ataaccgctc
     3961 aaaaagatat cttcttggat gttccgaaac gcatggacgt ctttgacact gcttaaaaac
     4021 gacaaacctt cacttcgaaa attaccgata tagactgctt cggtaaaggg gagcaaagcc
     4081 ggagctaatg gctgattttg ccgatggatt cctttgatat aaaacatcat tttgccagaa
     4141 gattccgtaa agattttgac taatttatct ttttctttgt aattacggct agataaaatc
     4201 aagcctttcg attctgcaag ctggctcata ttctccctcc taaaaaataa atctggaaaa
     4261 gcagattctg ccgatccaga ttatttatgt ttcgatcttc cgccgttttt ctcaacgtgc
     4321 ggatttttat ccagtttatt tctatactta acctataaga agttcagaat atcagtattc
     4381 gtcttttcgg tagccaaagt cttgcagata gacttttttg tctcgccagt ctttttggac
     4441 tttgacccaa agttctagaa atactttgtc tcctaaaaga ttttcgatat cctttcttgc
     4501 tttcgttcct acttgcttca gcatttttcc gcctttgccg ataatgattc ctttttggct
     4561 atctctttcg acgatgatcg tggcctggat atggatttta tcattctcgt ttcgcttcat
     4621 cgaatcaaca acgactgcga cagaatgagg cacttcgtct cgtgttaaaa acaagacttt
     4681 ctcacggatc aattcagaaa cgataaagta ttcaggatga tcagtcactt gatcatctgg
     4741 aaaatattgt gggccctctg gcatttcact gactaataca tccattaagc gttcgacatt
     4801 gtttccttct gtagcagaga tcggaatcac ttgtgcaaaa ggcatctgcg tcgaataatc
     4861 ttcgatgatt cccagcaact cgtctggatg gatcttatcg attttattga taatcagata
     4921 aactggagaa tgcacgtttt ttagacgttc aataataaaa tcgtctcccc ggccgcgttt
     4981 ttgatcagca ctgatcataa ataagactgc atccacttct ttcaacgcac tgtaagctgc
     5041 ttctaccata aaatctccta gacgatgctt tggtttatgg attcctggtg tatcgataaa
     5101 aatcagctga gcttctggtg tagtatatac tccttggatt ttatttctag ttgtctgtgc
     5161 cttgtcactc ataatggcaa ttttttgacc cacgattcgg ttcaataatg tggattttcc
     5221 tacattcggg cggccgacga ttgcgacaaa ccctgattta tgttctggca tatacgttct
     5281 cctttttaat taaacaacaa atgatatatt ttagggacaa ataaaatcaa cccaatgatg
     5341 acagcaaaac aagcggtcac taaaactgct ccagctgcca tatctttgat ttttttccca
     5401 atcggatgaa aatgaaaatc cgtaaacatg tccactacat tttcaaaaac agtattgatg
     5461 atctctacta tccaaacgag aaaaacagct aaaagcagcc ataaccactc tatccgatca
     5521 agctggaaaa tcacgcccat aaataaagca aggataccga acgcaacatg tttcctcata
     5581 ttgcgttctt cctcaaaaac ggttttgact ccttggacag caaattccac agacatcagg
     5641 aaatgtttgt ttttctctac gtttttatct tttgagtcca taggcatcca agatctcttt
     5701 ctgtaaacca aacatttctt tctcatcttc tggtgtcata tggtcatacc cgttgatatg
     5761 caagaatcca tgtaccgcta aaaagccaag ttcccgatca tatgaatggc cgtaagcttc
     5821 tgcttgttca gcagcacgtt caatagaaat catgatgtct cctagtacac gaggcaatgg
     5881 attctctcca tcttcaaaaa tcactgggat ttcgtcttcg ccttcttctt ccagagcaaa
     5941 actgatgacg tctgtcggtg catcttttcc acggtaatca cgattgatta cttggattgc
     6001 cgcattgtcc atgaaagtaa cagacatttc tgtttcctct ggcagcttta aataagatgc
     6061 tgcaaattgc agcaagtcat cgatttcttt cattttctct tctgagactt ggtttgtttc
     6121 atccataaag gtaatatcca ttatacatcg cttctttcta ttcttcttgt tcttccttta
     6181 tcttctcaac ttctgttttc attttaggat atttgatccg agaatggaag gtgctactta
     6241 aaccgctgat caatgatttt tccacttttc ggatttcttt caaggtcagt ccactatcgt
     6301 ctaattgacc atcggaaata cgctcttcga taagattatg gacaaaactt tcgatttttt
     6361 cgctcgtcgg atgatccatt gcccgtactg cagcctcaca actgtcagca atattgacga
     6421 tccctgcttc acgactttgc ggttttggtc caggataacg gaattgctct tctgttactt
     6481 ccggattgcg ttctttcgct ttgatgtaga aataacgcat tagcgtggtt ccatgatgct
     6541 gtttgcaaat atcgatcacc atttgcggca tattataatc ttccaatatt ttggctccat
     6601 ctgtcacatg tccaaagata atatgtttac tgtcttctgg caataagaaa ttgtgcgggt
     6661 tttccgctcc tgaaggcaag ttctcaacga aaaagctagc atgtttgatc ttgccgatat
     6721 catgataata gcaagctact cgagttagca aagaacgtcc gccgatttct gctacagcgt
     6781 tagcacttag attagctacc atcatactat gatgataagt acctggcgct tcttccagca
     6841 actgtttcaa taaaggatga tttggattgc tgagttcatt taatacaatt acactgtcat
     6901 cattgaccat caattcaata tatgggtgta gaccgatcgt cagcaagaat gagaagagtg
     6961 caccagtgaa cccgcagaca agcattaacc aagtcgttgc atcaccaaag ctcattcctt
     7021 gatagacgat caaaatgaca tccatcatca caggaaaaat aatgacccac atggcagcag
     7081 ataatccttg ttctgagatc cgtttgcgtc tgacaatcgt tgccatgagc ccagagaaaa
     7141 cataagacat caagataatc gttagaaagt tcgtgccaat cgaatcataa aaaatgaaaa
     7201 gtgcagatac tgcttggaaa ataccagcca tgatccctgc tcttcggttc aagaaaaagc
     7261 ttaaaacaag tggtgcaaaa gcagcgggat aaaacagcgg gatataagta gcttgttccg
     7321 tttggaataa ttggaaaaat ttcatcagca ctatgctgac tgacatagct gtcacataga
     7381 aaagcacgta ttttgttctt tctcctgcat tttggaattg aagtgaatta taaatcagta
     7441 cgccgatttg aagcaaaaca gctaaaacca tggcaattag cggaaaaatc gaagttgttt
     7501 gattagtcag tcctagtaat tccaattttt tcatagctga agcatcgatt tgattacctt
     7561 cacgaacaat gatttctcct tggaagatca tcactggttg tacagcttct cgtgcggatt
     7621 gtttcaattc ttctgtcttt ttctcattca atacgtcatt cactacgata ccttgatcca
     7681 ctaagtagcg aatcatttgt tgttgtgctg gtgtgacatt caaatattgg atttgctctt
     7741 ctgcttcttg tttaaaagct tccaattcac tttcacgaac ttgtttgctc atctgttcgt
     7801 caatgagctt cagactctca tccctcacag tatggatctc agtttcagtc atttcaaata
     7861 tcgtcgaata aaaattgttc ggtagttttt gataaaacgc aacattttct gcattcacat
     7921 tttcaaaatt tttcttcaac gcggcgatcc gctcttctgt agtaggcttt gggatatttt
     7981 catcttcctt tgccttatca atcttttcgt cataagattt gttgatggca tcattcgttt
     8041 tatcgatcaa ttcaaatagt tgcttgatcc gattgttctg gtcatctgca agatcttttt
     8101 ggtaagtgta ttctggagtg actgcttctg ctgccagttt tcttttttgt tcggtttctt
     8161 ccgtgttctc gatcgttttg ttcgcacgga tactttcttc tgctacttgg ccttctctga
     8221 aatcattggc cttttgtttg acactactaa atgtaacgcc gcagataatg ataaaaaaca
     8281 agaacagcag gaaaggcaat gctgcctttc caagtttttc ttgtagtttc tgcagtgttt
     8341 tattcaacat gtgcttatcc tcctaagcaa agcttattct tgatggagat ctgccttttc
     8401 ataagctttg atgatttcgg caactacagg gtgccgaaca acgtcacttg cttcaaaatt
     8461 aacaaaatcg attttcttga tttgctttaa tgttcgttct gcatgaacaa gaccacttgt
     8521 tgtccctcga ggcagatcaa tctgactcgt atctccattg acaatcattt ttgattgata
     8581 accaagacgt gtcaggaaca ttttcatctg agcaactgtc gtgttctgtg cttcatccaa
     8641 aataacgaat gcatcatcca atgtccgccc ccgcatgtaa gctaaaggag caatttcgat
     8701 cacgcctcgt tccatcaatc ggttcgtatg atctaatcca aaaatttggt ataaagcatc
     8761 ataaacagga cgcagataag gatctacttt ttcttttaga tccccgggaa gaaagcctag
     8821 attttcccct gcttccacag caggccgcgt caaaatgatt ttttgcactt cacctttttt
     8881 caaggcagcg attgctaata ctactgcaag aaaagttttt cctgtcccgg ctggtcctac
     8941 gccaaatacg atatcatgtt tggctaccga ttcgacatat ttcttttggc cgctgttttt
     9001 tacccgtatt ggttttccat tacggtcttt gacgatctct tcctcataca tttcaacaaa
     9061 ataatctaat gtgccttttt gccccatttt cagtgccgtc acgacatctg gtgtacttac
     9121 atggattcct cgtttgatca attcctgcaa tgtccgaatg actgaggcag ccaaggaaat
     9181 ctgggattgt tcgccaatga tctgaatgat ttctccacgt gtatggatgg ttgtttctgt
     9241 tgtttcttca atcagtttta tatgcttgtc gtgtgcacct aaaagcatgc tgacatcatc
     9301 agcagccgtc aatctaatct ctaaagaact agactcttcc gtcaaaaata gacatcccct
     9361 ttttttcgat tcattctttc ctattttacc atactcagaa gaaactatat agcaaataag
     9421 aaaaggttct aaaagagttg cttaaccata agtaccaaga aacgttaaaa aatagctgct
     9481 tccattctct aaaggtctcg tttgaataaa aaaagctgat catgtaagct atgcataaga
     9541 tagaggacaa gtattccgtc taacttagca agtattctct gggtattttt cttgtttcaa
     9601 aaaaagaaaa caaaatattt gaaatattct tgcatttcaa aaaaaacagg tgtattataa
     9661 cgtcaacaaa taaatataca gcaaagcgtc gaggaaaaca gtaggctatt cagcgattta
     9721 ttcccagtga gtcatgtaag tgagaaatga caataaatcg aatagacgaa catcatttcc
     9781 gagtcaatgt cgaaaacgta gatcattgtg gctgcctcca ttatcaggct agcggataac
     9841 tttatcgaaa agcgtccgtg aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt
     9901 ctagaatcta gagccctttt cccaagtgga aaagggcttt tttttatatt ttaaggtggt
     9961 ggttttcatg gaatctgata ttagtggcat ttctagtttc tgcaacgttt attttgttac
    10021 tcaaaatgat aattattgga ggaaaaattt atggaagaaa caaaaactta ttcatggaag
    10081 caattattga tggtcagttc acttatcttc gggatgttct tcgggtcagg taatctgatc
    10141 tttcctgtcc acttaggtca aatggctgga tcaaactgga tcagcgctgg tgtaggattt
    10201 gccatctcag gcgctctttt cccattatta gctatcttag ctgtagtagt gacaaaaagt
    10261 gatggtctgt atgacttagc taaacctgtc ggaaaatggt atgctgcctt gttcttgatc
    10321 cttgttcatt taacgatcgg accattcttt ggtacaccgc ggacagcagc aactgctttt
    10381 gaaatggctg ctaaaccctt tttgcctgaa aaatatgatt cattaggtat gtttttattc
    10441 tctgctcttt tctttttact tgcttatttc cttactgtac atccaacacg tttggtcaaa
    10501 tatgtcggaa agtatttaaa ctccttgttt cttgcattat tagctatcat ttttgtcgtt
    10561 tctttattcc aacccatggg gaatctaaac caatctgctg cagattctta tcagacgaac
    10621 gctttgacaa atggcgtttt agaaggatac aacacggttg atgcagtagc tttacttgct
    10681 ttaagtgtca cttttgtcca tgcagttcgt gatcttggtt ttaaagataa aaaagtcaca
    10741 gaattaacag caaaagctgg tagcctgagc gttgcgttag aagtcatgat ttattttggt
    10801 ctggtccttc tcggtgcgat gagtttgaat aagatgcctt tatcagaaaa tggcggaact
    10861 gcactttctc agatcactaa tttttattta ggtaacttcg gtgctagctt cttaggggta
    10921 atggtcacat taggtgtctt cactaccgct atgggactcg ttgtttcttt tgcacaagat
    10981 ttccataaac tgttccctaa agtcagttat atgacatggc tgcggttaac gacttttgtc
    11041 tcttttgtcg ttgcgaatgc cggattggat aatatcatcc aatggtcttt accagtattg
    11101 atgcttttgt atccattgtc acttgcgtta atcttgcttt ccttaacagc aaaattcttc
    11161 caaaagagtc catttgtcta tcaagtaacg atgttctttt ctgccgttcc agccgtattg
    11221 gatatgttgg caaattcgcc tgcattagtg agtcagcaaa ctgtcgtagc aagcttacta
    11281 ggattttatc atcatcacgt tccttttgct gcattaggac ttggctggat ggtcccaacg
    11341 ttacttggtt acgctggcag cttactcttt tattacgcct atcgcctatc agggtataaa
    11401 caagaagcaa atgaactacc agaagaataa ccaaatc
//
LOCUS       DAZULY010000083        11347 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000083 DAZULY010000000
VERSION     DAZULY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.084"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..496)
                     /locus_tag="ACG81A_001415"
     CDS             complement(<1..496)
                     /locus_tag="ACG81A_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS5014786.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGH"
     gene            complement(508..1200)
                     /locus_tag="ACG81A_001416"
     CDS             complement(508..1200)
                     /locus_tag="ACG81A_001416"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFS5014787.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1219..1503)
                     /gene="macP"
                     /locus_tag="ACG81A_001417"
     CDS             complement(1219..1503)
                     /gene="macP"
                     /locus_tag="ACG81A_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFS5014788.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1516..2079)
                     /locus_tag="ACG81A_001418"
     CDS             complement(1516..2079)
                     /locus_tag="ACG81A_001418"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFS5014789.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2307..2969
                     /locus_tag="ACG81A_001419"
     CDS             2307..2969
                     /locus_tag="ACG81A_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFS5014790.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3093..4289
                     /locus_tag="ACG81A_001420"
     CDS             3093..4289
                     /locus_tag="ACG81A_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFS5014791.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4390..5766)
                     /locus_tag="ACG81A_001421"
     CDS             complement(4390..5766)
                     /locus_tag="ACG81A_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5014792.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5803..6669)
                     /locus_tag="ACG81A_001422"
     CDS             complement(5803..6669)
                     /locus_tag="ACG81A_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFS5014793.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6758..7783)
                     /locus_tag="ACG81A_001423"
     CDS             complement(6758..7783)
                     /locus_tag="ACG81A_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5014794.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7801..8475)
                     /locus_tag="ACG81A_001424"
     CDS             complement(7801..8475)
                     /locus_tag="ACG81A_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5014795.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8659..9552)
                     /locus_tag="ACG81A_001425"
     CDS             complement(8659..9552)
                     /locus_tag="ACG81A_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5014796.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9626..11323)
                     /locus_tag="ACG81A_001426"
     CDS             complement(9626..11323)
                     /locus_tag="ACG81A_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5014797.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3366 a         2500 c         1953 g         3528 t
ORIGIN      
        1 catgtccaac gggatcgaaa cttgccacgg catctttgaa aatatggagt ttgtatccta
       61 gattatacgc atcgactgct gtatgcaaca cacagatatc tgtacatacg ccggtcaaat
      121 aaatatctgt gatctggcgt tctcgcaaac ggatgtctag atctgttcca ctaaaagcgg
      181 aataatgccg tttgtctatc caataaacat tttcctcttg ctcatagtct tggtataaac
      241 tttttaaaga gccgtacaat tcccgtccac ccgttcctat cacattatgc ggaggaaata
      301 agcggttctc cggatgaaag gaatcttttg gatcatgcgc atcaatggca aaaacgacaa
      361 aatccttttg atcaataaac ttctttgtat gagcagtcag ctttttttca attacttgtc
      421 ctgctgtccc agtagtcagc tttccatcct ctgcaacaaa atcatatgtg tagtcaatcg
      481 agatcaatgc gttcatctta gtcctcctta tacgagatgt ttgacaaatt cgatcaccat
      541 ttctgctgat cgtttgcctg cgtcttcgat aaattcgtca aagctttgtg tcgccgagtg
      601 atctgctgtg tcgctcattg cacgtacgat caaaaatggg atattgaact gacgagctgt
      661 ttgtccaaca gcggctcctt ccatttcgca agctaatgct tcagggaaat tcgttaagat
      721 ttctttgatt ttatctggcg agtcgacaaa tgtatcacct gtaacgatca atccttcttt
      781 tgcgttcaaa ttcgttgctt ttgctgcttt aaccatttca ctgcgcaaat atgtgcttgc
      841 ttcataatat agaggcattc ctggtaattg tcctggtttg tagccaaatc ctgttgcatc
      901 tgcatcaaaa taagctactt tgtctgatat aacgatgtca cctacttgta agccttcacc
      961 gattccgcca gctgaacctg tattgatcac catgttcacg ccgtattgtt ggatcaacag
     1021 acttgtggta atagatgcta agacttttcc tataccggaa cgtacgacga tcacttcgtg
     1081 gttacctaat gaacctgaaa taaataaagc gcctgcgcgt tcccatgata atggttcgct
     1141 taggttctct ctcaaaattt ttacttcttc ttccattgcg ccgataatac cgattttcat
     1201 ctttttaatt ccttctttct atataaatgc gattgctaaa aaaaccacac ataacattag
     1261 gataacaata ataagagatt tcatcaaaaa agagttccat ttggtcgtct tttcccgttc
     1321 gctgatcctt gttttcgtga ttggtttatt ctttttgctt tctttacgat aaaagctggc
     1381 aatctttttt tcttcttgct gataagctgt ttctgctttt cgctgttttt ttaatgactc
     1441 gcttgcttgt gcttgttgtc gcttgcgaag ttcgcttcgc gtaataagcg gtcctttggc
     1501 cataagccga tccctctact ttcctttggt caatagttcc cagtattgaa tcgcataaat
     1561 cgtctttgca tcacaaatca actgatcttt catcgcttgt tgcgcctctt caagagttag
     1621 atgatacaac tccagtactt cgtcttcatc ttgtgctaaa ggattctcga ccttctctac
     1681 cccttgtgca tgataaatat gaaggacttc atttgcaaaa cctggtgaaa gatacatcga
     1741 agtaagatgg ctcagcgact tcgcacgata gccagtttct tcttctaatt ctctagctgc
     1801 tgtcatttcg ggattttgtc cttctcctgg atcgattttt ccagctggaa tctccaaaat
     1861 cactttttct aaaggttttc gaaattgttt gacaagaagc agacgatctt gttcgtcaaa
     1921 agcaatgatc ccgacaccac caggatgaaa taccagttct ctcttcgaag ttcctccatc
     1981 tggcaaacga acttcgtcta cagcaacatc aataattttt ccgctataga tttctttgcg
     2041 actgattgtt ttttcttcaa attgctcatt gttcagcata tcttctcctg ctttctttgt
     2101 aatcataact gctttttttt catttttcaa tttttctcaa aaactcctta ttagtttacc
     2161 atagataaac cttagatttt ctctaaatat agaaaatcct tctttagtcc gatactcttt
     2221 ctagtagtcc gtatgctaaa ataagtatgt aatcgaataa cgaaaagaat tcaatactca
     2281 acgataaatg acatggaggg tttattatga taaaacgaaa ttggccgtgg cttctagcat
     2341 tggtcttgct atttctcttt gtccgaaaca atagtttttc ttttgttcta ggacttttat
     2401 tgataggtgt agttttagtt ttgtggggac tctttggttc gcgcaggaaa aagggaaaac
     2461 aagaagaaat tcctgcactg actgacgaac ttgaaactca ctatgaaaaa agcggtatga
     2521 cagcgaatga aatttcattt tttcgacaaa caatgaatca aacaaaaaat gagatcaatc
     2581 aattgcaaat gaatatgcag cagacagcca aacttaaagc aatcgatctc cgccatgatc
     2641 cagtcaaagc agcgaaagct ttattcaaag aattggtgaa agaaccaaac cgactgcatg
     2701 aagcaagtca gtttctttat acgcatctgc cgaatctagt tgatttgaca aataagtata
     2761 atgagatcaa tgaccatgag atcaaaaaca aacagaccta tgaaaaacta gaagaaagcg
     2821 cgcagatcat cgaccagctg tcagctttaa tcgcacaaga ttatcaaaaa ttcgttgctg
     2881 aggatctaga tgatatcgac gtagagatat ctgttgcgaa acagagctta aagcgtgata
     2941 ataaatatga accagaacaa aaggattaac tcttttgttc ataaaagaag actcggaaca
     3001 ttcagatgat gtctggctgt ttcgagtact ttttctaaag tagagacatt catgtaagaa
     3061 tcgagaacag attcaacaag gaggaatttt ttatggatca taaaccgact gaaacaaaag
     3121 atgttacacc agttaacgat acacttgaag atttattgaa caacccgttt tcaactccaa
     3181 cagatacgct gaccacttca caacaagcgg agattgatca gctccaagag caacagacag
     3241 ctgctcgttt aattgacaaa ctaccggcgg aacgccaaga acaagctaaa cagctagcag
     3301 caaaaatcga tgctaatgat gcacagtctg tcatcagcta cggatcggct gcccaagcaa
     3361 aattaagtga attctcacaa tcgatgttga atcatgtgca agcacaagat attggtcctg
     3421 taggagattc cttaactgaa ctgatgtatc gtctccaaga agccaatcct gacgaactgc
     3481 gtgccggaga agggaacatc ttccagcgtg tcttcgggaa ggtcaaacaa tcgatctatg
     3541 aagtgacagc aaaatatcaa aagattggtg cacaaattga caagatttca gtgaaattag
     3601 ataaagaaaa agacggtctg ctaaaagata atctgatgtt agaacagctt taccagaaaa
     3661 acaaagacta ctttgatgca ctgaatattt atatcgctgc tggagaattg aaaatggaag
     3721 aacttcaaac aacgatcatt ccagaagcga tgaaaagagc agaagaaact ggcgatcaga
     3781 tggatgttca gattgccaat gattacacgc aattcctaga tcgtctggat aaacgtacgc
     3841 atgatcttcg cttagctaga cagatcacga tccagcaagc accacaaatc cgtttgatcc
     3901 aaaacacaaa tcaggccttg gctgaaaaga tccaagcatc tatcgctaca gccattccgc
     3961 tgtggaaaaa ccaagtcgtg atcgcattga ccttgcttcg tcaaaaagat gcagtaactg
     4021 ctcaacgcca agtctctgaa acaacaaacg acttattgaa gaaaaattca gaaatgctga
     4081 aaatctcagc catcgaaaca gcgaaagaaa atgaacgtgg aatcgtggat atcgagaccc
     4141 ttcaaaccac tcaaaatgat ttgatcgaaa caatccaaga gacattgcgt atccaaaaag
     4201 aaggaaaaga aaaacgtcgt catgcagaag tagagcttgg acacatggaa gaagatctga
     4261 aacaaaaact gttggattta acccaataga tcacctttcg cttgttacta aaacaaacgc
     4321 ccaacaacag tttattacaa ctgttgatgg gcgtttttct tatctttctt ctaacttctc
     4381 cattggagtc tatttctcat tcatcttgct gtgtccatag ccatagccaa aataaatgat
     4441 cagtccaata accattacga taccgaacac gatccatgtc acagtttgca gacgaagcat
     4501 caatacaata caagtcaaga tggagacgat tggcaatagc ggaacgaatg gcactttgaa
     4561 ttctccagct tttggtttgc caaacatttt tctcaacttc agtaagccta gactaacaag
     4621 catcaaatag gcgagcgcca tgatattcgt tagttccgct aatacattca atgggaaaaa
     4681 tccggcaaag aatacagctg ccacaccagc ggcataagtc gcttttttcg gtgtacgatg
     4741 cttttgatcg atttctgtca taaacttagg aagtaagcca tccttgctga cagcaaacag
     4801 taaacgagca agtgaataca tcatcgagat cgttaccgtc agtagcgtta aaattgcccc
     4861 tacagcgatc accgcgccca ctgttccatg tcctacatac ctcattgcaa aagccactgg
     4921 gtcattgact cctagttcag tatacggaac gattcctgtc aagatcagcg tcacgatgat
     4981 gtaaaggaca gtagcaatca gtatcgaacc aatgattcct cttggcacat ctttttgcgg
     5041 gtttttgact tcctcggctg ccattgcaac ggcatcaaat cccaagaaag caaaaaacac
     5101 aagagctgca ccatctagca caccggaaaa accaaaaggc atgaatggct gccagttatc
     5161 aggtttaacg aaaaatattc ctacaatgat aaacaacgcg atgataccaa atttgacata
     5221 taccatggca ttattcaaac gtaatgcttt cttggcttca agcgacacga tgtaagtgac
     5281 gaatatcact acgagcaccg caatcaagtc aatgtatgta ccattttccg gattgtaccc
     5341 gccagttaat gcttgtggaa gttcggcccc taaacttttc aaaaaacctt gcatatagcc
     5401 agaccatccg gaagcaacag aggaaaccgc tagtaaaaat tccccgatca acaaccaccc
     5461 tgtcagccag gcagcaaatt caccaaatac aacatacaga taggcatatg gcccaccaat
     5521 cactggcact cgcgaagcaa attctgcgaa acttagtcca gacaaaatga caacgattgc
     5581 agctaagaca aatgaaagtg acagtgctgg accagcattt tgattggccg caacccctgt
     5641 caccacaaat attcccgtac cgatgatcgc accaattcca agcatgatca aatcagacgt
     5701 ttttagttct tttttcatct cactcgtatg attgagattg atctctttct tacgaaacaa
     5761 atccattgaa gttcctcccc attttaggtt ggaaaacagc gattaaactg ttttcatata
     5821 ccctaaaagg ataccaccta aaatgactaa aaggcaacca acaataacat aaatgagctc
     5881 ttttttcgtt tttctttcgc caaggatgaa aatcccacct aaagtagaga taatgatccc
     5941 catttgtgaa agtgagaagc caacagctaa accaagtgat ttgactgtta gaagcatgca
     6001 gatgtttcct aagccccata ataatccaca gatcatgttt ttccacacaa actgatcgac
     6061 tttaacttta cggaaagcaa agaagcttgc gcctaagatc attccgatac tttgaggtaa
     6121 gataattgac atcgcatcca aattcgccca gttgacgatg atggtatata caccataacc
     6181 aatcgtagag aagatcaatg ctctaaaacc cttaccaaaa tcaagcattt tattatctgt
     6241 atctattttt ccttcgtcat cttgtcgggc agtcaaatac gcaccgctta caagaagagc
     6301 taaagcaata ataccgtata cataatcttt cgtttgtgtc cattcatgga agaaaacggc
     6361 tcccgcaatc gtattcaaaa ttaattgaaa accagtagac aatggtagcc ctacagaaac
     6421 acccatgtac ttcattccat ggaactgtcc attttgtcca acactccaag ctaagccgga
     6481 taaaaatccg atcaagaaaa tccaaggggt gattacagga ctaacaacga aaaagacaac
     6541 taaagaaaac agaaatgcac cgatcgtcat tcctagtgtt tgttgattgg catcgccacc
     6601 aattttacca cttactaaac caatactacc ccatgccaac atagggacta atgctattaa
     6661 aatttccata accaatctcc ttttatttca catgataaga aacttatcag cgaaaacgtt
     6721 aacaacacaa gcactttttt tcgtttctaa cgattttcta acgtttttca ttataaaaat
     6781 ttaagattgg gaaaaataaa gtcattgttg tcccttttcc ttcagttgat tcagccgtca
     6841 aatggatacc taatttttcg gccagatttt ttgctaaata aagtcctaat cctgttgaat
     6901 gtgttttgct aagtcgacca tttctcccag taaatccctt atcaaagatc cgtttcaagt
     6961 cttccgctgg gataccgatc cctgaatctt taactgaaag gctgactcct tgcgcttcct
     7021 tttctatgct cacaatgatg tttccgtgat ccggcgtata tttgatggca ttactaagca
     7081 attggttgaa gataaaagcg atccatttag catcggtcaa aacagtatga tcctcaccaa
     7141 cgatctcgta gcgaatattt ttttggataa aataatttgc ctgactgcgt atcacaggct
     7201 gaatgattcc ttttagtcca tattcttgga tgaggtagtc ccgcgagaaa ctgtcgagtc
     7261 ttgcataata caaaacttgt tctacgtatt catcgatttt tgataattca ttttctagta
     7321 atgtatactt ttgatcgtca atatcgaact cgatcgaacg aactagcaat ttagaagcgg
     7381 aaagtggcac tttgatttca tggacccatg agtcaatata atctttttgg tcttgttgat
     7441 tactgattgc tgattgcatt acttcttggt gttcacggat cagctgattg atatattctt
     7501 ctaccagttt ttcttcttct gtcctcccac cactcaaata attttgcaaa ggggaagttt
     7561 tttgtatgtc ggctaatttt ccccaccatc ttctcttgct caagtaacca cccagcagat
     7621 aaaaaatcag aaagacgccc tcaatcaaag ctaaataagc aattgtgctc aaatcaactt
     7681 tgatgtttgg cgccagccac atcacaaaga tcgtcaatgc aataaaaaac agccatccca
     7741 agatcaatgt ccactgatct ttcaaatatt taaaaaaatt catcttaacc gctcatcctt
     7801 tcagggaacg atatatccct gtcctacttt tgtttcgata tatccttgga tacctgcctg
     7861 ttcgattttt cggcgtaacc gattgatgtt gactgtcaat gtattgtcat ctacaaatcg
     7921 ttcgtcatcc cacagtgctc gcagcaattt ttcccgactt aagatctttc cattgtgctt
     7981 catcaaaata aataatagac gatattcatt tttgctcaaa tcaatgatct ctccgttgat
     8041 ttccatactt ccgttgtcaa cattcaacgt aatgccattg tggctcatca tttcactaga
     8101 taattctgtg tagttgtaag aacggcgaag taatgcattg atttttgcaa tcagtacatc
     8161 gatttggaaa tgttttgtca caaaatcatc tgcccccatg ttcatagcca tgatcatatc
     8221 catattcgta ttcctgctgg agataaaaat gatcggtact ttcgatactt cacgtatttt
     8281 ttgacaccaa taatagccat ccaaaacggg aagattaata tctagtaata caagctgtgg
     8341 ttcttctctt tcaaagtctg agaacacttg attgaaatcg gtcgttccaa aggtttcaaa
     8401 ttgccatttc tcaagttcct ccatgatcaa ttgtcgaatg atctcttcgt cttcgactac
     8461 catgattttt gccataaaaa atccctccat ctttcttctt gctactacaa tatcaaagaa
     8521 gcccttgtga aacaagttgg cttacctgga aaatgtcatt ttagcaaacg ttaaaaaaaa
     8581 ctggaaatat gcgatttaat cgcgtttgtc tcttccaagc agcttaaatt tattatgtta
     8641 ttcttaataa ttggtctatt atctgttatt tttctttcgt tgtttttgtc ttttaaattt
     8701 tcgataacac caataaccga aaagcgttag cactagcaat atacctagaa ggagtataag
     8761 taatcgataa agatggtatt tttttgtctg ttctatcttt gaagagactt cttctgtaaa
     8821 gggaactcgt cttcctgtca ctagtaatcg gtgagtgttc acggtgtaag gtgtacaagt
     8881 gataagtgtg actagatcct gcttttctct aattgctaat gtatttgttt ttgttggaag
     8941 gacaacaatt ttctcaacga cttcataagc gagcatccga ccatacactt caagatagaa
     9001 tcggtcatct tgctccattt cttcaagatc cgtaaagagt tttttctcag caataccgct
     9061 atgggcggta atcacggaat gtgtactctc tcctccagtt ggaaaagagg tgccttgcag
     9121 gactgtcgct cctctatcta acaaaagatc attcgtttca tcaaataaag gcaagctaac
     9181 attgattttc ggaatataga tggctccgat catatgttgt tcgtaatatt cttttcctgg
     9241 attttggaca tttctaaccg cttgctcaaa aggatctttg acttgtccca ttcctgggat
     9301 tactgatgta tgagcaagcg tctgattttt cttttcatgc actgctaatt gttgctcatg
     9361 tttttcttct ttatcgcgtt caagctgctt ttgataattc tcgatccttc tttgatccaa
     9421 ataattgctg agagcatctg ctaaaaatgg atatgtaaaa actgcagctc cggcaagaaa
     9481 catcactgcc ataaataatt gtaataaaag actgatttgg gtttgaggtc tattacctat
     9541 tttctttttc attttcttca ccttcttatt taaaaaaact gcagcaacct ttataaaacc
     9601 gattgctgca gcttattttt gttgcttata cttcagactt cattttagat ttacggtacc
     9661 agctgtacgc tccaatcatc aaacttattc cgattagtaa gaatgccaag agcccgttcc
     9721 caccagtagc aggaagtgac ccgcgtttca tattcggtac tctttctggc cctggatttt
     9781 ctactcctac gatctgaatt tcttcattat agcttccata agagatgata aattcagtaa
     9841 aggctgcctc acctgttgga agtacataac cttctggcgc catggtctct ttcatttgat
     9901 aggttccata atctaaccct tgaacctcaa agtatccatt tccatctgcg acaaattctg
     9961 ttgcttgtcc gcttgttgcc cactcaattc tatctgctcc tgattcataa acagctaaag
    10021 gcgactcccc tttataaaat attgcgtatt cagaacggtc ttcattccac aattcgaatc
    10081 ttgcatcttt aagcaattca ctgcttgatg catcatattt ttcaaacttt cttccacctg
    10141 ttgtgacttt ctctggcggc tctactcctt cttcataatc aaattcatca tttcgtccga
    10201 tttcaataac cgctgtgttg ttatggaaat catccgcttc tagttctgtg gaaaccttca
    10261 aattatacgt gatcgttaaa tctcctccac gtgcatctgc taatctacct aaagtatcta
    10321 ctttggctac tccattttct tctgtcaaaa tcgtcacgac aaacccgttg cttactactt
    10381 ctactatata atcttgtcct tttgttaaaa tcgtctcgcc ggctcgcact tctatttcct
    10441 gatcataata agttagacca actgtaggcg tatctgtgat tttgtaattt ttgataacag
    10501 cgcttccgtt ttcaaggaca gaatcgatga aataaggaat aggtgctgtg atcgtgtaag
    10561 aagccgtttt tcctctttct agatcaatat aagaaatcgt ttcttgttct ccagcttcat
    10621 taataatcgt tacttgtcta aggtcgcttt cctctacatt catctgttta tctgcttctc
    10681 tgatttcgtt tttcggatac aaatgcacat ctgtattgta tgtgtctttc catgtagaac
    10741 catcccacac gccatccacc atatcaggca tcatcactgg catcataaca accattggag
    10801 acgcgattct ggaaatatga gctggcgaat aagtttctaa gaaaagatag actttatctc
    10861 tagaagaagg tgcttcttcc gtcacttgga cggtagagaa taccgcttct ccagcttcat
    10921 tcgttgtttg tttgtcaata cttgtcagtc gatattttaa aaaccaactt tctgtgtgcc
    10981 gtttttgaat ccattcaatc gcttcggcag aattaagacc gtcttctcgt tccggtgtta
    11041 aaggattatc tcgaatcaaa tcgtaatata cttccgttac gtcaaacaaa tcaaaatcga
    11101 ctcctggaat attttcttca ccaaaatccg tagaaaccaa tccgctattc ggtcgttctt
    11161 ccggaacaga tgagaaacct tttttgtgta atgtaatcgt cacttcactt ggtttttgtg
    11221 cgacttcttc ggctttgact agttttccgt tcccgaaaat ccctattccc gcagtaacga
    11281 gtaacagtac actcattccc gtgacaatca tttttttatt catttttttc tccccgttct
    11341 tttttta
//
LOCUS       DAZULY010000084        11252 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000084 DAZULY010000000
VERSION     DAZULY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.085"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            238..444
                     /locus_tag="ACG81A_001427"
     CDS             238..444
                     /locus_tag="ACG81A_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFS5014798.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(646..1647)
                     /locus_tag="ACG81A_001428"
     CDS             complement(646..1647)
                     /locus_tag="ACG81A_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5014799.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1652..3565)
                     /locus_tag="ACG81A_001429"
     CDS             complement(1652..3565)
                     /locus_tag="ACG81A_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5014800.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3733..4239)
                     /locus_tag="ACG81A_001430"
     CDS             complement(3733..4239)
                     /locus_tag="ACG81A_001430"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFS5014801.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4399..4839
                     /locus_tag="ACG81A_001431"
     CDS             4399..4839
                     /locus_tag="ACG81A_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5014802.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            4998..6338
                     /locus_tag="ACG81A_001432"
     CDS             4998..6338
                     /locus_tag="ACG81A_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5014803.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(6407..7522)
                     /locus_tag="ACG81A_001433"
     CDS             complement(6407..7522)
                     /locus_tag="ACG81A_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014804.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(7567..7923)
                     /locus_tag="ACG81A_001434"
     CDS             complement(7567..7923)
                     /locus_tag="ACG81A_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014805.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(8221..9195)
                     /gene="sgrA"
                     /locus_tag="ACG81A_001435"
     CDS             complement(8221..9195)
                     /gene="sgrA"
                     /locus_tag="ACG81A_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFS5014806.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(9391..10230)
                     /locus_tag="ACG81A_001436"
     CDS             complement(9391..10230)
                     /locus_tag="ACG81A_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFS5014807.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            10415..>11252
                     /locus_tag="ACG81A_001437"
     CDS             10415..>11252
                     /locus_tag="ACG81A_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFS5014808.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMK"
BASE COUNT         3387 a         2297 c         1760 g         3808 t
ORIGIN      
        1 ttcttttggg ggtatcctaa atcttttcta ttatattccc atattttcta ttattggtct
       61 agacgtatga tggtaaactt tgtacctatc tgattgctga atcattaatt gcttatcatt
      121 agccatcttt gtttcaagtt cgctagatga tcattttatt cacatcctaa caaacttgtc
      181 acatttatag attcttaatc tatttatcat acaattataa aaaaggaggt gctgcttatg
      241 aaagctatcc atatttcttt gctttttttc gctagtgcat tgattttatt tgctattcat
      301 ctttgttccc agccttatat caataacaac ggaatattag tggaaccagc attcttttgc
      361 ttaccactcg gctttttgtc ccttcttgcc tccgcaggct tcggatttgt tgcttttttt
      421 aaacgatcaa aacaacaaat ctgacttata aaatttacgt aaaagagatc gtgacaataa
      481 tcgtgtgata ttcccaaatt tcgaataaaa ggtgcccctt gccatctcta actcctcagt
      541 cacaagttac aatattcata aaccatgaaa agcttgttta ttgaaattgc tccttgtgac
      601 ttggagctaa gtgacagatt acaacctctt ttgcatcata agactttact ccttgaaaaa
      661 cattactaac ttttgatatg tttcttgcgc ttgtttattg ctgaaatcaa actgatattc
      721 atggttcaaa acagcatttt tctccgcata atctactttt gtgactttca cccccgcttt
      781 ctcaaataca gaagttactt cttccatttg tttcgtaaac gtatttgtat ttccatccgt
      841 taagaatgtg ttttgcgtcc aacgatccgc atgtctgacc aaggctaaac ttttgacctt
      901 ctctgattgt tcaaagtcct tattatcaaa gtaagcccat ccacattttt cataaagaaa
      961 gctagagatt ttatcactta cttgagtaag tgcagaagga tccaacagac cagacaaaga
     1021 aataaacttc gtaatctgct tgttctccaa agtcggatca agatgatcag cttttttaat
     1081 ttcttcattt tgaagtgcta acaaatattc tccactaatt tgtgcacctg ccgaatttcc
     1141 accaatcgaa atattcgacc aatctgcttg atcttttatc tcttttttag ctgcttgaat
     1201 ggcttcgttc aattggataa ctggtgtagg atgttttgct tccggtgatc gttcataatc
     1261 tatatttacg atgatttgct gcgtgtctga acatagatat tccaaataat cacgacaatc
     1321 atttttatcc cctcctacat atgcgcctcc atgcatccag aaaagaatcg gtaatttttt
     1381 cttttccact ttcggtgtgt agatatccat attattattt ttgtactttg aaggatagga
     1441 tatttctttt tttatatcga tttgttcgat tttttgctgg aaatcatctg gtcgggcata
     1501 tacttgttct ttttcacttg tgtcaaattg ttttctaaca agcaagctaa tcggctttgg
     1561 tgtgatttga ttaatcaaaa atacagtgac taaaataact acgatgataa ctggtactgc
     1621 aatcactatt cgcttgaacc atttcattca tttaccctct ctttcttcgc caaatccatg
     1681 tgataatcgt gctaacagat aaaaatccac ctgaataaat gagaagcgaa tgtttctttt
     1741 ctccagtttt cggcaacatt cttttttttg catctgtctt tttttgtact ttcactgtca
     1801 tctgaatcgg ttgcgtatca tcacttgatt gatcctctcg aggttcattc tttcccttat
     1861 tgtctgcatt ctctgtcgga tcttgctttt ttggtggagc tactgggatt tttttgtaat
     1921 agaaaataat agttggtatc gtctcattaa aaatcccttc agtcggtcct tcaacatttg
     1981 ctaattcgta tccttcgaat gtcttttgct ctgtcatata attttctcca taagctccgt
     2041 aaagctggat ttcgtcctgg tctttgagta gacttttatt gttttcatcc acatacttta
     2101 cgtttatctg atcatggaac actactttca aaaagtccgt tacatgtttc gtgtaggcag
     2161 catggtcaac ttcctgtgcc tttccatgcc ctgctccttc tactaataaa agatctttat
     2221 accctcctga taactcatat aatttttgcg cgttagacac aggtactaca tcatctgctg
     2281 ttccatggat aatcaattta ggcaatgaat ctgacattcc aatcgtaggt agagcagctt
     2341 ttgtatccaa atgcatctcc tgatcaaaat agtattgttt caaaaggccc ataaataaat
     2401 cttcatgctc ttgatcccca atgatgtcta actgattttt tatggcatcc ggtagtgcgg
     2461 gggctactgt ctggctgtac gtaaaacgta gttgttcatc aattgatgca aatccgcaat
     2521 tttcaataat tcctttgaca ttgacaggaa tatctttcgc taatacagac atcatcgtag
     2581 ctgctcccat cgaaccacca taaagtaaaa tcttttgaga tggccgctct cgaacttctt
     2641 gatttatcca gtaaaggacg tcatctgact catattgtcc ataggtcaca taatcgcctc
     2701 cgctattacc agtcgctctg gaatcaacaa ataataaatt atacccggcc ttataaaaat
     2761 cattgtattc ttctgtaacg atcccgttat tccaattccc tcgaaatccg ccatggatca
     2821 tgactgtttt atctgaacct tgatcgacat aatatgcata tacatccatc attttgccat
     2881 cttgtttgtt tagtacagtt tgatgaagtt gttttgtatt tggatagaag cctgtgtttt
     2941 ctgtaggcgt tgtcccatca tctgtacctc caagcatcga catcaacaca ctgttttttg
     3001 caaaagcgat atcgtacaat agataagtag caatcagatg tttgataatc tctttatcta
     3061 caccataatc attaacagca agctcagtga ctaataactc tattaatttt gacttgatgt
     3121 cttctgttgg tccattaagt atttctgtca tctgtttacc gaagtaattc aaatattctt
     3181 taccattgaa attttcacct ttaccgatat catcattttc tgtcccattc tctttgtcac
     3241 ttactttagc agaagaggat tcgtcgtttg acatttttgt agctgttgct tcttctgttt
     3301 gttggttatc tgtggtagcg gtcgtttctt gatctgtgct agtaacagat gtctcaaccg
     3361 ttgtttcagt atttgtttct gtttgtgaag tactcgattt cgtttcattt ccagcatcag
     3421 tagtttctgt ggtttctacc tcccgagatt ccgattcgga agaaatgctc tctccatata
     3481 ctgtccattg cgggacgctt gtacttacag caaataagat gaccaacgct ccctttaata
     3541 cttgttttgt tctcaagcta ttcactcctt tttatgtttc taggagaatt atatgtaaac
     3601 gcaaacattt tttatacaaa aaataaatgg tttatcccat aaaaatagaa caggattatt
     3661 ttctcatttt acgtaataaa gcagaatgga taaaccagta tccattctgc tttttttaaa
     3721 cgaagaaaca acttattgct ccaaaaatgt cagaaatcgt ttatcccaaa ttttctcgta
     3781 aaaagcgatt gtttcctttg ctttcataaa aggattatca taagttgtcg ttgctcctct
     3841 acacatcata agctggtatc ctaatccatg agcaaacttc acagtcgtat ctatacaata
     3901 ctctgcttgt gccccacata attcgatcga atgacagtcc aattcagtta ataaaacact
     3961 caaattagtc ttataaaatg aattcgcatg ggtttttctc acaaaataac tgtgctcagg
     4021 aatagataat tccgacaata gctcccatgg ttttgtttgt ggcactaatt gagcatcttc
     4081 gtgttgaacg aaaataactg gtttcccaat cgtttcgtat gttttgattc ttttattcac
     4141 taatgctgtc actgtttcta attcgtgtat cggcgaagaa tcaaaacata cccctttttg
     4201 taaatcgatc actactattg cgtcggctgt gtttgacata cttgtgcctt ctttcttttt
     4261 tcttgattta atagtatttc ttatctagct agttccatta tacgtgacaa cattttctta
     4321 ttttacacct ctaaatggca cttttcgact atttcttcat gttattattt cataaatact
     4381 tagctattgg aggaatgtat gatgattaaa ttgtatacag gttctggctg ctcgtcttca
     4441 agacgcgcaa aacgttggct tatcgtccat gatataccgt ttatcgaaca gaacttaatt
     4501 cgtcaaccac taaccaaaaa tgaatttatc cacttactaa aattgacaaa caacggcgtg
     4561 accgaaatgc tctctactca ttctattagt tataaaaatc ttgttcacag aatagatgaa
     4621 ctcagtttga gtgaactata tattgagatt cgtacatccc cttctttatt gaaaaaaccg
     4681 attatctacg accaatctgc aaaacttttg actggattta gcgaagataa aatcaggatg
     4741 ttcctttcta aaactaaaag aaagcaagag ctcaacgaac tcttgctttc tggtcatctt
     4801 gtcgatttta aagaaaataa tctttttgca atgtcttaat tttggcataa acaatctgct
     4861 tttctggatc tataatatct agtgttggtg acgccttctg ttgccaatac tttttctttt
     4921 ttctaataaa cgcaaacaga caccttgaaa tcctttagaa taaagtcgac gaaaaccatt
     4981 caaaggagtg tttcaagatg tcctattcag agtttaccaa agagttacta gatattctag
     5041 acctaaatct tacctttcat gaagatgcct ttcgcaaaga gcgcattaat gacgaaacat
     5101 gttttgtttt tgatggcaca ttgacctatc aaccagagga atgcttccat tgccactatc
     5161 aaaataagca aacgatcatt aaatggggct ggaaaaaagt atccatctta ttgaatgatg
     5221 tcagtaacta caagaccatt gtgcggatta acaaacagcg cttcaaatgt aaacactgtg
     5281 gaaagacttt tttagcagaa gattcagtta gcgatcgtcg ctgctccatt gcccgcagag
     5341 tcaaacaagc cattcttgag ctattaagtg agcctatttc tatgtctttg attgcacgta
     5401 tgaagcacat ttctccgacc actgtcattc gaatacttcg cagccttcgg cctaaaaccg
     5461 tttctttgaa ccagccgtta ccagaagtgg tgtgtttcga tgaattcaaa tcggtgaaaa
     5521 acgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
     5581 ttgtggaaaa tcggcaactc aatcctttac gcgactattt cttgcgctac cctagaaaag
     5641 tccgtgaaca agtacgtcta gtggtttcag atttctatac accctatcgc acattggtga
     5701 aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacatcg
     5761 gtcgggcctt taccaaccac cgaattcaag taatgaagac ctttaaaaaa ggggatcgtc
     5821 ggtcgaagca cttaaagaaa tactggagac tacttcaaaa gaatgcgtgg gaattgaatg
     5881 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagcg gagatagtcg
     5941 atcggctatt atattatgat gactccttaa agcgaggcta cgaagtctac caggtttttc
     6001 tttccgctat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
     6061 aggagttgcc agagcactat caaccagtgt tcacgacctt caagaaatat cgaacagaaa
     6121 tcaaacgagc gttgcgagtt ccctattcca acggccctat agaatgtttg aacaaccata
     6181 tcaaagtact aaaacgaatt gcctatggct ttagaaactt tcaaaactac cgagaacgaa
     6241 tctttttata tcgaggaaaa tatttcaaga aaactaagaa cactacccaa ctgactaaag
     6301 ccagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt
     6361 ctattgaatt tcttcaccaa cactcattga cgaagagccg cttttcttaa ttaacgaaat
     6421 ccaatttttc caatatggat ctatttttct cattcctgat ttttatgatt tcagctttca
     6481 gacgcgccca atcatttttt gtaggttgtc cattccagta aaatatctgt gtattcgttt
     6541 gatcatatgc ctttttgtcg ataggataat cgctaatgac aatatcaggt aactgatcag
     6601 cggatgcact atattttatt gggcgttcag tcatagataa tatccttttc tttgcccata
     6661 attcctgtgt tttaccatac tttgattgaa tatagaatat gacaggttgt tcgtgtttta
     6721 taaaaatatc cctgactaac atacagtatt gaaaaatcaa ccgtctgttc ttttcataat
     6781 aagtattaag ctctggaatt ctgcgagcta ccccactaaa aaactgttgc atgattggat
     6841 acatcactgg aaatatatgc tgaaaatctc tgtcttctgc tgattttcca aaagcatcca
     6901 ccttcttttt tgtcccaaaa acccttgaat acaaatggat cattgccaaa ttgacatata
     6961 gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt tttttcaaaa ttttctacaa
     7021 tagacgattc aatccagccc caactttctt gtactttatc tggagtatcc aacaaatctt
     7081 cttccaaata ggtgttcatt acaccaaaca taaaataaag gtaagcttgc tctgttggag
     7141 tgatgtgatt catcgaccag aagtttgctt caaaaaaagg ctctagtatg taatgcgtaa
     7201 actcttcttt tgttatcaat ggatgcacaa atttgttcat ctcttctggg acatacgtaa
     7261 tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag taggctgatc catttctttc
     7321 tatctatact acgcgtatat ggcgcatgtt tcgcaaattt ttctaatagt tgacgtttat
     7381 tttttatact tgataatata tcagaatgat tttcgtcgaa tgactgccaa tacatcaagt
     7441 gaaaaaagta tcggatacgg tattcttctc cttctatcgg attacctttt ttcaagctgc
     7501 aagataaatg atagtttttc aaaatagtat taagttctct taattttctt tgaatcgtac
     7561 gcaaacttat ataatttttt gactaagatt ttctaaggat tgataagtac ctttgtaaag
     7621 tgaatccagt aaaatataac caatcgttcc ctctaaaagt atagaataaa ctttactcag
     7681 taaaaactga gaagaaaact cgacttttat catcttgttt tctgaaagaa aagataattt
     7741 atattctgga tacctttcgc aaaacgattc tgcttgttga atcaccgaat tcatcttagc
     7801 cagggtgtag tttagatcta aagcgatatc cttcaccaca tataatttct cgtctctttc
     7861 ataacaatag ttcaaaaatt gatatacttg cctttcattg atatcaataa attcttcaaa
     7921 caaagtacat actccttttt atctattatt tagacttatt ccgacagcta catgaataac
     7981 gtacaaatag attcgttact gatttttcag agatactacc atgcgcagtg tgcattgtca
     8041 attattcttg ctcttatttt ccatttgtgt tcctgttatt tcctgtttct caaatatgtt
     8101 agtaaacaaa aatagatctg gcaaagctac aacttcgcca ggcaatacag cactctcgaa
     8161 tgaaatattc actcaaaaat ctatcgtttt attcaatttt agatctgttg cttttattct
     8221 ttatttcact cgatggaaga gagaacgaaa atagcttgct cctacaatca aaatgagtaa
     8281 cacacccatc catgtaatta gcttgttcaa ggttctacta ccagtttctg gtaaatcgct
     8341 ttttctatta tctgtttctt tcgctttttc ttgatgatca attccacgag aaacaaaatc
     8401 aggctgtttt agcacacttt ttaggttgct ccctgcattt ctcttttgtt ccttagttgg
     8461 tatgataggt accaaatcaa gttcattatt tacttttcct gtgctgcttt cgctgctcgt
     8521 gcttcctgtg ctactctcac tactcatgct tcctgtgcta ctttcactac tcgtactctc
     8581 cgtactgctt tcgctgctcg tgcttcctgt gctactttca ctactcgtac tctccgtact
     8641 gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc gtactctctg tactgctttc
     8701 accgctcgtg ttttctgtac tgctttcact acttgtgcta tccgtactgc ttggcggtcc
     8761 aacaactgtc caactgcttg gtttcaaatt ctccgtgatc gtcatctgtc catcataaaa
     8821 aacaatcaaa tcaactttat ctttactctt gatctcatca atcgatagtt ctgcagaaat
     8881 cattattgta tactcaatac cagcggtcca ttgtacgtct tttaaataaa cttccccaat
     8941 aactttttct gaacttccat taccatccca accatatgtt agaacagcat gtacatcact
     9001 agcattcacg cttttgtttg gtttgactgg aatagtaatg acttgttgtg tcatgtcata
     9061 aacaatccga tcagttttca cagtaaatga aagattctca tttgcccgtt catttgcaga
     9121 agtactgatt ccaatcattg ctgcaattat actcagccca agaataacaa ataatacggt
     9181 cgctgttttc ttcatttcta tcactctcca tataattacc ttctatttga taaaggaatt
     9241 atataaaatg aaaatgatga gaacacgaca gtcagttgtg aaaatctaac aaaagatgtc
     9301 gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa tgacagactt aaaaaagcaa
     9361 gaccgatatc agccttgctt tcttcatctt ttattgataa acagcagtgt atttcttcat
     9421 ccgctttgtt ttatatgcat agagtaacaa accaacaatc aagactaaca agaataatgt
     9481 agttgatata gatgcttcag ccccaaagtt tcccccagta attattgtat tttgcgattg
     9541 gatcagagtg gagaaaaaac cttgttcttg cgttccgctg actggtatac ccaaaatagc
     9601 tccttgtgtc caattccaag taatatgagc accaatagcc gtaaataaac ttcctgtact
     9661 gtagataagt gcgctgaaaa agcaacctgc taaaaagata ttgaaaactg ccagaaatgt
     9721 catgttagga ttcaatccgt gcaaaagagc aaataatgca gcagatccaa tcattgctat
     9781 taacggacca aatctaagca tctgatgctg aaaaaagcca cggaataaaa gctcttctga
     9841 tgcccccact gcaataaata aaataaaact cgttatcagc ggcacaatgt ttgcgtttcc
     9901 tgaatctaac cgatacgccc ccgtcaaata taatggacta gtaataagca gaaacactcc
     9961 aattccaaca acgagacctc ccgctaaata cccccagtct tttcctcgaa cagaaacacc
    10021 ataatcttct attctttttc cttcgacctt actagcaaga aataaagcta gtaaaaaaac
    10081 agctaaatgt ccgaccgtac cggcaattgc tccaatccat tcactatttc caataaacat
    10141 atccgcaagt gcattattga caagaattcc taaaacaaaa ccaactaaga ctattaaaac
    10201 agatccaatc aaccgaataa tcctgatcat ctttcatccc tcctttatat taagtttaac
    10261 aacccttttc tttcttgtct cgtgaaaact atgtaaatgt tctccatcat agagagaata
    10321 gatttttacg aactaagaat atctacagca accttacgaa aatgaaagac tgtctcccta
    10381 attcttctgt tagactgttt ttaataagga aaggatggga aattttttga aaaaacaact
    10441 actaattatg tgcctttcag catgtgcaat tttactattc tcaggttgta gcaagtccaa
    10501 tgaaacgatt gcaacgttta aaggtggaac acttacagtt caggattttt atgataaccc
    10561 aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg attgtcttca aagcatttga
    10621 tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca aaagaatata atgaacaaat
    10681 caaaaaactc ggtccaaact acaaggaaca attaaaagca gttggccaaa cagaagagac
    10741 atataagaaa ctctttaaac agatgctggc tttccaatac ggtttgaaag ccaatgtaaa
    10801 attaacagat aaagatctag acactgcttg gaaagaattc tatccagaag ttagtacaca
    10861 gatcattcta ttttcgacta aagaagaagc agataaagca aaaaaagaag caaatgaagg
    10921 ggaaaatttc tccaagcttg ttcaagctta tggaaaaaat aaaacattaa aggaaactga
    10981 tggaaaaatg aattttgatt caaccaatcc agagattcct actgaagtaa aaaaagcagc
    11041 atttaaacta aaaaacgggg aagtttctga tatcattcct gtaacagatc caacgactta
    11101 tcagcaaagc tattatctag taaaaatggt aaaaaaacag gataaaggtt ctaataaaga
    11161 caaatacaaa tctgagctag aaaaaattgc taccgaagca aaaactcttg atacggaatt
    11221 catggataaa acaatccgta aagtgatgaa aa
//
LOCUS       DAZULY010000085        11246 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000085 DAZULY010000000
VERSION     DAZULY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.086"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..978)
                     /gene="murB"
                     /locus_tag="ACG81A_001438"
     CDS             complement(43..978)
                     /gene="murB"
                     /locus_tag="ACG81A_001438"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFS5014809.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1051..1971)
                     /locus_tag="ACG81A_001439"
     CDS             complement(1051..1971)
                     /locus_tag="ACG81A_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5014810.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2084..2836
                     /locus_tag="ACG81A_001440"
     CDS             2084..2836
                     /locus_tag="ACG81A_001440"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFS5014811.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3700..4233
                     /locus_tag="ACG81A_001441"
     CDS             3700..4233
                     /locus_tag="ACG81A_001441"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS5014812.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4723..5247)
                     /locus_tag="ACG81A_001442"
     CDS             complement(4723..5247)
                     /locus_tag="ACG81A_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFS5014813.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5240..5713)
                     /gene="tsaE"
                     /locus_tag="ACG81A_001443"
     CDS             complement(5240..5713)
                     /gene="tsaE"
                     /locus_tag="ACG81A_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFS5014814.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5836..6816)
                     /gene="pta"
                     /locus_tag="ACG81A_001444"
     CDS             complement(5836..6816)
                     /gene="pta"
                     /locus_tag="ACG81A_001444"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFS5014815.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6969..7649)
                     /locus_tag="ACG81A_001445"
     CDS             complement(6969..7649)
                     /locus_tag="ACG81A_001445"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS5014816.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7775..8644
                     /locus_tag="ACG81A_001446"
     CDS             7775..8644
                     /locus_tag="ACG81A_001446"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5014817.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8670..9185
                     /locus_tag="ACG81A_001447"
     CDS             8670..9185
                     /locus_tag="ACG81A_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFS5014818.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9232..9795)
                     /locus_tag="ACG81A_001448"
     CDS             complement(9232..9795)
                     /locus_tag="ACG81A_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5014819.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9850..10029)
                     /locus_tag="ACG81A_001449"
     CDS             complement(9850..10029)
                     /locus_tag="ACG81A_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014820.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10106..10615)
                     /locus_tag="ACG81A_001450"
     CDS             complement(10106..10615)
                     /locus_tag="ACG81A_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014821.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10711..11091)
                     /locus_tag="ACG81A_001451"
     CDS             complement(10711..11091)
                     /locus_tag="ACG81A_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5014822.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
BASE COUNT         3220 a         2308 c         1996 g         3722 t
ORIGIN      
        1 ccaccgtcct gctcttttac ttattttaag ttccattact tattaattgg ctaaacttct
       61 gcttgtttct gatattgctt gtttttctcc aatgatccgc acttctgttt ctaaagacac
      121 atcaaatttt tctttgatta cttcttggat gtgcgcaatc aattcaatat aatctgtcgc
      181 tgtcgcatga tcgatattga cgatgaatcc agcatgtttt tcagagactt gtgcaccgcc
      241 ccatttcagg ccttgcaatc ctgcttgttg gatcagctga cctgtatagt gtcctactgg
      301 gcgcttaaag acacttccgc atgaagggta ttctaatggc tgttttgatt gacgcaattc
      361 ggtcaactca tccattcttg ctttgattgc ttcataatcc cctgtttgca aactgaatcg
      421 agcttccaat acgatagcac gtaattcttg gactttactg tgacgataag aaaataccat
      481 ttcgtctttt gtcaaagttt tcaatgttcc atcttcaagc aaaagatcca cttcagcaaa
      541 aacatctttg atctctccgc catacgcacc tgcattcata taaaccgctc caccgacact
      601 tcccgggatg ccgcaagcaa attcaaatcc agtcaatgat tctgccaaag ctgcataagt
      661 cgtatcaatc agtgaggcac ctgcttccgc aatgaccatc gttcctttga catgaatctg
      721 tttcatttca gtcagcatga tgactacacc gcgaatcccg ccatcttgaa cgatcagatt
      781 gcttgcattt cctaatacca tccaaggtat atcatggatt cggcaataat cgatgatttg
      841 ctttacttct tcttgttttt tcgggaatgc aaggacatca gctggtccac ctgttttcgt
      901 aaacgtatag ttcatcaatg gttcatcaaa caatatagta atcgtggcta agctttcaac
      961 tatttcttct ttattcactg aggattttcc tttcttttct caaacgtcct cattattcta
     1021 tcacgcttaa tacaatcttg ccagtcttgt tcaaagttga tgacggtagt gatctttaag
     1081 cacttcctca cataattcat gacttcttac aacttctttt tgtttaagca attctgtttc
     1141 cgatttacct aaacggatct gagaaaagaa ggcctctacc attggctcaa acccgcgttt
     1201 gactagcgtg tttgtccagt cgccaaattc tttcactttt tttccatcag cctcttgaat
     1261 gacaagctgg gtcagatcgt ttacttcata agtaccattt ttacttgtta cttgatattt
     1321 ttctatatta gcgccacttg ctaaatccat cgttaaaata gccgtttgat tttgtgtttc
     1381 taattgtaaa atggcatatt ccaaaaattc ctttgtttct cttatctggt attttgttct
     1441 caaaatgggt tcatctaata aatacacagc tgtatcaata agatgtaaaa acaaatcata
     1501 aaggacgaac tctgtcgttt cttttgccgc tattcgattt ttttgcaggt ggatcaaccg
     1561 cttatctggg atacgtttca attcttctac cataggagca aagcgccggt tgaaaccgat
     1621 catcaaaatt atttggttct tatcagctaa ttcttgcaat tcacgaactt catttagatg
     1681 gacactcagt ggtttgtcaa tataaacatg gataccattt tctagacatt ttttggctaa
     1741 ttcataatgt acttttgttg ctgcgtggat catacatgct tcgatttttt cttctaaaag
     1801 ctcatcgatc gtttcatata gatgttggaa cccgtactct ttttgaattc tttcttttgt
     1861 ttctttattt cttgttgcaa aataaaaatc aagtgttcct tgttttttcg cataagttgg
     1921 taaatatgct ttttctgcga tattccctac accgattaca ccaatcttca tctactttcc
     1981 tcctggatca tttagttact ttcatcttac accatttttt actaaaatga ggtacaatag
     2041 tatcgcaaag gtaaggaaat taattcgaag gagtgtgctt ttattgaaat ttatctcttg
     2101 gaacgtaaac gggcttcgtg ccatcgtaaa taaaaatttt ttagaagttt ttcatgagtt
     2161 agacgccgat tttttctgtc tgcaggaaac gaaactccaa gcaggacaaa ttgatttaga
     2221 tcttccaggt tattatcaat attggaatta cgcggaacga aaaggctatt ccggcacagc
     2281 gatttttgct aaaaaaccag cattgaatgc cacttacgga atgggtatcg atatccatga
     2341 tacagaaggc cgtctgatta ctttggaata ttccgatttc tttcttgtaa cctgttacac
     2401 gccaaattct caaagcgaat taaaacgttt agattaccga ttagaatggg aagaagcctt
     2461 ttataattat ttggaaaatc taaaaaaaca aaaaccagtc atcgtgtgtg gggatctaaa
     2521 tgtcgctcat caaaagattg atttgaaaaa ctggaaaacg aatcaaaaaa atgctggatt
     2581 cactccagaa gaaagagcgg ctctttctcg actgttggat aacgggttta tcgatacatt
     2641 ccgttatttt tatccaaccc aggaaggtgt ctactcttgg tggaactacc gattcaattc
     2701 aaggaaaaac aatgctggtt ggcggatcga ttactttttg acgagcaaag atttagagcc
     2761 aggactagct gatgcaaaga tccatacgga cattatgggc agtgatcatt gtccagtaga
     2821 attagatttg aaataaagag gttgtgaaaa agtcgtttag tttcgagtat taagaaaaat
     2881 tttgtgaaaa tggctttcca cattttgcat aaatttggca taataccgag aaactggctt
     2941 ttgaacactg tttatcgaga gattgtgaaa aagctgtcct gcatcaagta ataagaacag
     3001 ccaaacaaaa atagttcctc atatttttcg tttggttggg cttattaccg cagatacaag
     3061 cttttgaaca ccgtttatta agaggttgtg aaaaagctgc actgcatcaa gtaataagaa
     3121 cagccaaaca aaaatagttc ctcatatttt tcgtttggtt gggcttatta ccgcagatgc
     3181 aagcttttga acaccgttta ttaagaggtt gtgaaaaagc tgcactgcat caagtaataa
     3241 gaacagccaa acaaaaatag ttcctcatat ttttcgtttg gttggactta ttaccgcaga
     3301 tgcaagcttt tgaacaccgt ttattgagaa gttgtgaaat cagcgtcctg acctgagtat
     3361 tagagaaaca aatggcaaaa tagttcctca tattttgcta ttttttgagc taataccgag
     3421 ggtcagctga ttgaacaccg tttagtggag aagttgtgaa aaagtcgcct actttcgagt
     3481 cgtaagaaat ttttttgaaa atggttcctc acaactttgc ataaatttga cttaataccg
     3541 agaagtcatg atccccatct aactccaaat cacaaggaat gagttcaaaa actagctttt
     3601 ctcaacaaat attacataaa aaattctatg ttttttgtta aatttatttg cttttttcaa
     3661 aagtatgtta cagtaatttc ccaaggaggt tttacagaaa tgaattttgt tgccatggat
     3721 tttgaaactg ccaatcatca gccttatagt gcttgttctc ttgcgctagt catggtaaaa
     3781 aatagccaga tcgttgatga attctataca ttgatacagc ctgagactcc ttttttctgg
     3841 cgaaatgtcc aaattcacgg aatccatcaa gaagacgtac gcaatgcgcc gaaattccca
     3901 gaagtctggc aaactatcca accatatttt caaaaaaatc gtttagtcgt tgcacataat
     3961 gctgcttttg atacaaaggt cttagcggga tgcttagatt attatcatct tgaacagcct
     4021 aattatcttt ctttatgtac agtgaaaagt agccgtcgcc tgtttcctga atttccaaat
     4081 catcgattga acactgtgtg cgaaaatttg aacatccaat tagatcatca ccatgatgca
     4141 ttggaagata gtcgtgcatg tgcggaaatc ctgctttatc aagaaaaaaa attcgggaca
     4201 gatccattga aaaaacttgt gactataaag taaaagaata cttatgaaac acacaaagag
     4261 gtcgtgagcc tgccgtttca ctccgagtca caaggagcaa tttcaaaaaa tagcttctca
     4321 tgtttattaa atattgtgac tggtgaccga ggagctggca ggagaacacc gtttattgga
     4381 gaggttgtga aaaagctgtt ctgcatcaag taataagaac agccaaacaa aaatagcttc
     4441 tcatattttt catttggttg gacttattac cgcagatgca agcttttgga caccgtttac
     4501 caagaggttg tgaaatcagc gttttgacct gagtattaga gaaacaaacg gcaaaatagt
     4561 tcctcatatt ttgccgtttt ttgagctaat accgaaggtc agctgattga acaccgttta
     4621 ttcaaaaaaa atggactgcg acacgacttt tgtcacagtc ccttcgtgtg tttttctgcc
     4681 ttctcaaaag gatgacagtt tctcactctt ttcctatttc ttctattcaa tcaaataact
     4741 cattttatat acatctaaaa attcacctag atctgttctt gcccctctag gcatgaccgc
     4801 ttcgatttgg aagcccattt ttcggtaaag gtgtacggct ctttcattac gaacttgaac
     4861 gtcaagctcc aatcgaaaaa gtacacccat ttccttcgcc caactgatga tttcttctag
     4921 cataagagtg cctagtccca ttccccaata ttcctgcaga atactgatac cgacttctcc
     4981 cacgtgagac aaacgaaatt gactgtctgc cttgacagat gctgttccaa taatcgtccc
     5041 ttcatagatt gcaagcagca ataaattatt attgctttcg tatatataat ccaactcttc
     5101 ttctaaggtt tctggtggta acaggagttc tgcttcatcc aaaactaaaa attctgtctc
     5161 cttattaact tgtcccatca tcaaaagaat gtcttttgca tctcctggca ctgcttcccg
     5221 caagatgatc ccaacttctt cactcatgat tttcagccca tttttctaga attcgctgtt
     5281 tgaaggtttc actttgctcc ccgtatgaga ttagtttcac ataccgcttt tctaaatctg
     5341 tatcactttt ttctaaaatc agttccaaat aatcctccgg cagtgcttct tctaatagat
     5401 ttccccattc aacaacggaa agcccatcac cttcaaaata ttcatctaac cctaaatctg
     5461 ctccgctagc tgctacccga taaatatcca tatgatacaa cggaatccga ccttgactat
     5521 attctctaat aatcgtgtat gtcggacttt tgatcatttg cgttatccct aatccttggg
     5581 caattccttt cgtcaacgtt gttttacccg cacctagatc accagtcaat atcagattgt
     5641 cacctggcat tgcggaatga ccaattgcct ctgcgaattg ttctgtcatt gttaatcctg
     5701 atagttcgat catttttcat cttctcccgc tcttctttct ttttgccagt atatcgcgta
     5761 atgaagtttt tttaaagaaa aaatgaacta gtcaagaaca gcagctgctg tttttggcta
     5821 gttcgcgtgt atttgttaat caatcaatgt ttgtgctgct gtgataatcg ataatttata
     5881 tacatcttct tcatttgctc cacgagacaa atcagaaacc ggtttgttca agccttgtag
     5941 aattggtccg attgcttcaa agttaccgaa acgctgagca attttatagc cgatatttcc
     6001 tgactgcaat tctggaaata caaagactgt tgcttgacca gctacttctg aatctggcgc
     6061 tttcagttgt gcaactgaag gaacataaga agcatcaaat tgcaattcgc cgtcgatcat
     6121 gtattgtggc gctagttttt tagcgatttt agttgcttca actactttgt ctacttctgg
     6181 cgatttcgct gagccttttg tcgagaagct catcattgct acttttggtt cgatatcgaa
     6241 caattctgct gtttttgcag aatctacggc gatttcagcc aattcttgcg cagttggatt
     6301 cacgttgatc gcacaatccg aaaagacata tttttcttga tctcttccac gtaccatgat
     6361 catcgctccg cttgtccgac tgatgccagg ctttgttttg atgatctgaa gtgctgggcg
     6421 aacagtgtcg ccagtcgaat ggatcgctcc agagaccatt ccgtctgcaa tccccatata
     6481 cgtaagcatc gtaccaaagt aattcacatc ttttaaaatc ttttctgctt gttctttgga
     6541 tgcctttcca tttctacgtt caacaaaagt atccaccatt tcatcccact tatcgtaatt
     6601 atcagggctg atgatcgtaa agctagatgt tttgattcct ctggcatgcg ctgcatttct
     6661 tatttcttct tcgttcccta ctaaaatggg ttccacaagt tcttctgctt tcaaccgggc
     6721 agctgcgcct aaaatacgag catcagtagc ttccgggaaa acgattttga tcccacggcg
     6781 aacaatttta aatcttaaac tatcaaacaa ttccacaaga tgacctccag acgtttttat
     6841 atgtattatc atacactatt ccagaaaaaa agaacagttt attttatctg tatcagtcaa
     6901 aaatgctgga aaaattaagt ttttcacata caaagcgaac tgcactataa caggaaagcg
     6961 ctgttttatt acactgtttc aggcagttgc caatcaatcg gttcttctcc taacgcaagg
     7021 agcgcatcat tcgttttcga gaagggtttg gaaccaaaaa agccacgact tgcagataac
     7081 ggactgggat gtggagatgt tatgatgata tggcgactct tatcgatcat tttgattttt
     7141 tcttgtgccg gttttcccca caagataaac acgattggtt tttcccgttc atttaatttt
     7201 tcaatgatcg tgtctgttaa tctttcccaa cctttcccgc gatgagaata cgcttgccct
     7261 tcccgaacgg taagaacggt gttgagtaaa aggactccct gtttagccca tgacactaga
     7321 tttccatgtt gaacaggtgc gattccaaga tcatcataaa gctctttata gatatttctt
     7381 aacgatggcg ggatctttac gccaggttgc acagaaaaag ataagccatg agcttgattt
     7441 tctccatgat aaggatcttg tcctaaaatc accactttaa cttcttcata aggagtcaat
     7501 tccaatgctt catagatatg atacatatct ggatggatct tttgggacgc atattctttt
     7561 tttaaaaatt ctcttagatg cagataataa tcttttgaaa attctgagga caaaacttct
     7621 tgccaactgt tatgaatgat ttctttcaaa aactccacct cctaataaaa agaataactc
     7681 acattttctt caatcacaag ggacaatctt caccctcagc acttgattaa tatggtaaac
     7741 taggaacaag aagcagaaag gaacgataat cattatgatc aaattaattg catcagatat
     7801 ggacggcaca ttattagatg cccatatgag tgtttctcct gaaaatgccg aggcgatacg
     7861 ttttgcaaat gatgccggtg ttgagttcat ggtcgctact ggtcgaaact atcaagaagc
     7921 tcgcgcagct ttagatgagg taggaatcga ttgtgcgatg atcactttga acggggcaca
     7981 ggtatttgat aaagaaggaa attcattgtt taccgtgtcg attccaaata atcaagcaat
     8041 ctctgtttta gatattctag atgctaacgg tatttactat gaagtagcaa ccaacgatgg
     8101 tctttattct gaaaatcaag cgaaacgaat tgaaagtttt gcttccatgg tggctaccca
     8161 tttacctcac cttacgtata aaatggcgat tgccatggcc tcagctaagc tggagttatt
     8221 gcacatcaca tacgtggaca gtattcgtgc tattttggaa gaaaagaatc tggaagtttt
     8281 aaaaattatt tgttttcata cagaagggcc agctgtatta ggtccagttg gaaagcagat
     8341 ttctctttta ggcgatctag ccgttacttc ctctggtcaa aacaatatcg aagtgaacca
     8401 taaaaacgct caaaaaggaa ttgctgttgc ccatgtcgct cacgagcgtg ggatttcttt
     8461 agaagaagtc atgacgattg gcgataactt caatgatgtc agcatgctgc aagtagcagg
     8521 agtcagtttc gcaatgggaa atgcagaaat cgaagtaaaa gattatgcca aatatacaac
     8581 ggacacaaac cttgaatctg gcgtaggaaa agccatattg cgcgcgattg cagaagactt
     8641 gtagttttat ctagaaaaga gggatataga tgtcagaatt tttcattcag gaaaagcagc
     8701 ttggacgtgc aactcggaca attgtcaaaa acgaaaaagg acattccctg tttttgatgg
     8761 ttggtcgctg gggaacaaag ggcgatgcat tgtctcttta tgccatgaat ggcgatctcg
     8821 ttgctcacat caagcagata agcctgacat tcggtacgcg ttttgagctg tataaagagt
     8881 tcgaaaaggt gggaaccatg cagaaaatat tcaattggcc cggtgacttt tattatattc
     8941 gtagacttca ctggacagtc caaggtgata tctataatca tcaatatcaa atccatcatt
     9001 tcaatcagca aatcatgaaa atggataaag caacactttt ttcaggagat tactatgtat
     9061 tggatgtctc aaatgatgaa gatgctccag tttgtatttg tattgctgcg gttttagatt
     9121 actggctcta ccgtaaagac aaaagtcgcg ataagcctgt ttcttaccga cttaacttaa
     9181 gttaaaaaga tgtacctgaa atgcggaaat acgcatttca ggtacatctt tttagatatt
     9241 cttttgcata tgatcataaa gatgaccact gatcatccgc tgaccgacaa ctttgaagtc
     9301 atggcgttcg tataattttt tagcctttgg atttttttca tccacactta gaccgatgac
     9361 ggagcgatta gctttcttcg ccaatttagg taatgcttct aataatcgcg aaccgatgcc
     9421 ttgtcctcta aaatcttctg atacactgat agagtccaaa taccattcgt tgggaaacgt
     9481 ttctttatca atgaacaaac gtgtagacgc atctagctga tatttctcta agataggtac
     9541 cagtggctga tcgatgattg attcttcttc atctgtataa ccgtaagcaa ctcctgcaac
     9601 tttaccgtct atttcttcta cgatacctcg tgaataactg taacgataat ttggatcagt
     9661 gattgcttct tctaaaatct ctaaagtctt ttgttcccct gtctgcttca aaaatggcag
     9721 ttccatatct tttagaatca ctaagatcaa tggggcgatt tctggagcat cttcttttct
     9781 cgcaaaacga atcatagttt cctctccttt tctccattct aacatgcaga aaaagaaatc
     9841 gcaaaatcgt tattttaaaa aatcttcaat cttttcagta atgaaataac catgagtaac
     9901 gattcttgcc gcttcagttc ggcaataagg gcagacgact ggcatttctt tatcattatg
     9961 ttcgactaca ttattgactt cttcgtatgc aaattttttt ccgcaattag ggcatttcat
    10021 tttttccatg ttatctcaac ttcctttcat ctttactctc ttagcttact cttatattgt
    10081 aaaaaagaga agcaatatcc ttcttctatg cagatatcca tacctgacgg tcctgttctc
    10141 catctattaa tcggacttta taataaggac gttcatcttt gactagaagt gtccactctt
    10201 tcacttttgc ttttttcccg tagtagctga ctgcttcctt catcgctgct tgcggttgtt
    10261 tgaactcttc cgttttttct atatcaaaaa gatgttctgc ggccttttcc gtgtcatttc
    10321 gtacggttgt tttcccagtg gatggattga ctgctatgac ttgtccttca caaaaaaact
    10381 ggtattcata tgttgcattc gtaggattct ccaaatcttt ttcaactgca aaatgaatag
    10441 cagatatgtt atgttctttt gtttcgtttt ttacgattgc tgctgctttt ttcaatgaga
    10501 tccgatattc ctggtcacta atatcatcca tgtgaaaagc ggcattgagc agtcccgtca
    10561 taccggtaat ccctattacg atgagggcag caacaccaaa catcattttt ttcattcttc
    10621 attctcctgt ttcttttttt taaagaatat caagaaatga acgaatgaaa aatcgaatga
    10681 aacgaaaaaa aagtgacaat tttgttatct ttattcgtgc agttctaatg gcaatccgtc
    10741 tggatcaaag aaaaacgtca ttttttttcc tgtaaaggta tcttctctaa tcggttctga
    10801 ttcgatccct aaggactgca attctgcaat tgtttcttca atatcgtcta ctttgaaagc
    10861 aagatgacgc aagccacaag cttctggata gtttggtcgt tgtggcgcat tagcaattcc
    10921 aaaaatctcc aattcagcac ttcctagttt cagatctaat ttatagtcat ctcgttcttt
    10981 gcggtaattt tctctgatta cttcaaatcc taatttatta acataaaaat cacgtgattt
    11041 ctgataatct gaaacaatga tggctatatg atggatcgtt gtaaggttca tatgagcact
    11101 cctattcttc gtttaagctc ggatctatat taatctccag ttccattgta aatgtgtcag
    11161 attgaaaaaa atagctgttt ttccttttta taagaaaaaa ggcaaaaaaa acagaaagta
    11221 ggtcctccaa gacactcttc ctgttc
//
LOCUS       DAZULY010000086        11069 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000086 DAZULY010000000
VERSION     DAZULY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.087"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..1354
                     /gene="efmA"
                     /locus_tag="ACG81A_001452"
     CDS             68..1354
                     /gene="efmA"
                     /locus_tag="ACG81A_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFS5014823.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(1492..2091)
                     /locus_tag="ACG81A_001453"
     CDS             complement(1492..2091)
                     /locus_tag="ACG81A_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5014824.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            2495..3616
                     /gene="mnmA"
                     /locus_tag="ACG81A_001454"
     CDS             2495..3616
                     /gene="mnmA"
                     /locus_tag="ACG81A_001454"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFS5014825.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            3862..5283
                     /gene="arcD"
                     /locus_tag="ACG81A_001455"
     CDS             3862..5283
                     /gene="arcD"
                     /locus_tag="ACG81A_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFS5014826.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            5297..6415
                     /locus_tag="ACG81A_001456"
     CDS             5297..6415
                     /locus_tag="ACG81A_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFS5014827.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(6475..7767)
                     /locus_tag="ACG81A_001457"
     CDS             complement(6475..7767)
                     /locus_tag="ACG81A_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFS5014828.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            7917..8477
                     /locus_tag="ACG81A_001458"
     CDS             7917..8477
                     /locus_tag="ACG81A_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5014829.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            8606..8983
                     /locus_tag="ACG81A_001459"
     CDS             8606..8983
                     /locus_tag="ACG81A_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFS5014830.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            8916..10988
                     /locus_tag="ACG81A_001460"
     CDS             8916..10988
                     /locus_tag="ACG81A_001460"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS5014831.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         3571 a         1991 c         2396 g         3111 t
ORIGIN      
        1 aaaagaatcc cgtataatga ttgttgtgca aacgctatac aaaatgaaaa agaaaagagg
       61 gatgtttatg gaaaatgaac agtcggtcgt tttaacgaat tggaagagaa attatttgtt
      121 ctttttatcg ggacagtttt tgtcaggaat cactagtatg gtcgtccaat atgcgatcat
      181 ctggtatttg acaagagaaa ccggttcggc gacaattttg agttttgcta ctttactagg
      241 aatgattccc atggttttac taagcccatt tgtaggaccg ctagtggatc gctgggataa
      301 aaaagctctt ttgattgtca cggatattat tgtagcgatt ttcgcgctta ttttagcagt
      361 agtcggaacg atttcagaat ccttcccgat ttggctagtt tttgtgtctt tgtttatgcg
      421 ttcggtcgca caaacattcc aaatgccaac gatccagtcg attatgccga caatcgttcc
      481 atcttctcac ataacaagga cgaatggaca gttaggaatg gtccaatcag cgaatttcat
      541 cattgcgcca gctctaggtg cggctttgtt ttcagtcgtt cctgtcaatt accttatttt
      601 attagatgta ttgggggcag tttttggtgt cggcttattg atttttgtga agatcccaaa
      661 agtttctcct gaaatattgg aagttccgct tactattttt aaagatgcaa aatttgggct
      721 gcagcaattg atggataata aagggttatg gtacataacg atcaatggtg catttgtgat
      781 gctgttgttc atgccagcaa tcagtctgta tccattgatg acgctagatt actttggtgg
      841 ttccgttgga caggcgggag cagtggaagt agtctacgca gtcggaatgc tcttaggcgg
      901 tgcactgatc agttttatag gaacatggaa agaccgaatg aaacctatta ttatagccta
      961 catcattatg ggcctgacga tcggtgcaag cggattggtt ccaaacgata gtcaagggtt
     1021 cttgtacttt cttatcttga atgcaggagc aggttgtgca acgccatatt tcaatacact
     1081 gctgatggca atgatccagc agagttatga atcgaatgtt ttaggtcgtg tcttagggaa
     1141 cttcaactct ttgatgaacc tgacaggtcc aattggatta ttatttgcag gaccattagc
     1201 tgatcgttta ggggtagaaa aaatgttctt gttttccgga atcgggattt tattatgcgg
     1261 aatcgtttta tttttaacgt cagctgcccg aaattatgat aaagaattac agaaaaaact
     1321 ggtaaaagaa caccatgagc aaaaagacga atagtatgga atggagttgt gacagaagtc
     1381 ttgttacaac tccatttctg atcctaatgt tcctgtggca ataagcctac tcaaatgaaa
     1441 aatatgagta attatcttta tttgtttaat acttgatata gggcagtttt ttcacgacct
     1501 atcgtacttc ctctttgctt catagatttc tttgatttgt tgctcagata acggagtgcc
     1561 aaatggtagt ttttccttta agagttctct ttccggtgcg tctacgccta cattgacatc
     1621 gttctcgata ggtacgctgt aatgatggat atgtccatga agattgcgga tagtgtcgtt
     1681 aggcccgagc aacatcggat aatgagtcaa gtaatattgt tgatggtcga atttaacgat
     1741 ggcacctaca tcataaaaat aaaatttggg acttccgtca gtaagcctag gatcattttt
     1801 ttctaaataa cggaaaaaag cgcgactgtc atggttccct ttgatcaagt ggattgttcc
     1861 ttgcagttga gtgaccagtt ccaaaatttc tgcatttgct ttttcatatt gcggatgcat
     1921 agcaaggtcg cctagatgat aaacatggtc ggtttctttt attactgcat tccagctagt
     1981 gatcaactgt tggttcattt catccacatt tgcaaagagc cgcggagcaa agtcgttttt
     2041 tcctaataag cgttcatgga aaaagtgcat atcactgata aagtaattca tgtatctttt
     2101 gcctcccttt tttcttagag taaacaaaat cacttatctg agcaactcat aacatctaaa
     2161 aagaaaaggt tgtaaatgag acgtgcagct ttaagcgata agaagcaatt ttgaaaaata
     2221 gtttttcaca atttataaaa ttattcctta ttgccgaaag gctggccctt tttatgagta
     2281 tgccaatttt ttatatcgaa tgctcaaaag atgagtgatt ttttggttga ctatcatgtt
     2341 tgtgtactga ataggtccag tagaacagta tttttacgac ttgataatta agggatgaaa
     2401 ttttcagaaa tatgacgtat aatgtaaaat actgaaaagt tgcgaccttc aactcgtaga
     2461 tgtttcagaa tctaattgaa atgatggtga tatcatgaca gacaacagca aaacacgcgt
     2521 tgtcgtaggg atgagcggcg gtgtcgactc atctgtaaca gcacttttgt taaaggaaca
     2581 aggatacgac gtgatcggaa tattcatgaa aaattgggat gacacggacg aaaacggtgt
     2641 atgcacggcc acggaagatt ataaggatgt agcgaaagta gctgctcaaa tcgggattcc
     2701 ttattactct gtgaattttg aaaaagaata ttgggatcgt gtatttgaat atttcctagc
     2761 cgaataccgc gcaggacgta cacctaaccc agacgtgatg tgcaacaaag aaatcaaatt
     2821 caaagcattc ttggattatg caatggatct tggcgccgat tatgtagcta ctggacatta
     2881 tgcgcaagtc actcgcgatg aaaacggcgt ggtccacatg cttcgtggag tagacaataa
     2941 taaagatcaa acttacttcc ttagccaatt atcacaagaa cagctttcaa aaacgatgtt
     3001 ccctctaggc ggcatggaaa aatcagaagt acgggcaatt gctgaacgcg caggccttgc
     3061 gactgctaag aaaaaagatt caacgggaat ctgctttatt ggtgaaaaga acttcaaaca
     3121 atttttgagc aactatctgc cagcaaaaaa aggaaatatg gtcactcttg atggtgaagt
     3181 caaaggacaa catgctggct tgatgtatta tacgattggt caacgtcaag gactagggat
     3241 cggtggcgga ggagattcgc aagaaccctg gtttgttgta ggaaaagatc tagcgacaaa
     3301 cacattatat gtggggcaag ggttccatca tcctgcattg tatgccacaa gcttggatgc
     3361 aagtgaaatc cactttacga cgaatgaacc aatgccaaaa gaattcaagt gtacagcgaa
     3421 attccgttat cgtcagcaag acgttcctgt gacagttcgc ttattggatg ataatcgtgc
     3481 agaagtcgta ttcgacgaac ctgtacgtgc aatcactcct ggacaagcag ttgtatttta
     3541 tgatggaatg gaatgtctgg gtggtggttt gatcgaccac gcatatcaag aaacaaaagt
     3601 tttacaatac gtttaagcga tattaaatga atcaaaacag ctatggctaa caaaagaaac
     3661 accttttctt ttggatagca tagctgtttt tttgcaataa aattaatttt tattcatttt
     3721 atacttgaaa tacttgtgta aaaagttcat aatatccaat aatatgaata aagttgcttt
     3781 ttgttaagca agagcagttg caaaatagaa gggaatcttt tgcaagtgat ttgttcaatt
     3841 ttataagtga gggtgatgga aatggaaaag aaatcgacgg gtattagcaa atggggactg
     3901 gttgctctgg ttgtcagttc gtcaatcgga agcggtgtgt ttgggatcac aagtgatctg
     3961 gctagttctg cagctccagg gccagcgatc ctttcatgga taatcgttgg gattggtatt
     4021 ttagctttgg tattatcgct gaatcattta ggagaaaagc gtccagatct agatggtggt
     4081 attttcggtt acgcggaagc ttcatttggt aaattaggtg gcttcatcag tggttggggt
     4141 tactggttgt ctgcgtggct gggaaatgtt gcttttgcaa cgatgctgat gagcgccatg
     4201 ggagaatttt tcccgatatt caaaggtgga caaaatgttc cttctatttt atttgcaagt
     4261 atttttattt ggggtctgac tttattagta aacaatggag ttgaaggagc gagcgtcatc
     4321 aatacaatcg tgacgatctg taaattagtg ccattattcc tatttttaat atttgtattt
     4381 atcgcattta aagtgaatat attcactgct gatttttggg gtaacgtatc tgataatcta
     4441 gtgaatggaa cagggcagca aggaacgatc tggttgcaga ttaaaggttg tttgatggtt
     4501 atgatgtggg tctttgtagg gatcgaaggc gcaagtgttt tagccaaccg cgcgaagaaa
     4561 cgttcagaag cccagcaagc atcgatcatt ggtctgcttt gtttattgtt tatctatatc
     4621 cttgcttcga tcttgccata cggcgtctta tctcaagaag agttagcaac tatttcacaa
     4681 cctgctatgg caaatatttt aaaaggaatc gtgggaaatt ggggagcagc gctgatcaat
     4741 atcggcttga ttatttcgat cattggttct tggttgtctt ggacgatgct cccagcagaa
     4801 acaacgatgt tgatggctcg agacggtctg ttgccgaaag gttggggaaa aacgaataaa
     4861 aaaaatgccc caacttattc gttagttgtt actgcaatct tgactaatct attcttattg
     4921 actttcttag ttactgatta tgcctatcaa tttgcctatt ctttatgtac agcggcaatt
     4981 ttgatttgtt atctacttgt cgggatctat caagtccagt tttctttaaa agaaggaatc
     5041 aaaaaacaag tagtgatcgg tgggattgct gtagtttttg aattgatggg catcatttta
     5101 gcaggatttt cttatgtcct tttatgtagt attgcctatc ttcctggttt ttgcttctac
     5161 tggatcgctt gtcgtgaaac caaacatcaa attacgggaa aagaaaaagt aatgattggg
     5221 ctgatttgtg tggccgcact gctttcaatc gggttgcttg ctgtaggctg gatcaagata
     5281 taagggggaa agaaacatga aaatcgcaaa tttcggtgta gaagaatggc taaataaatg
     5341 ggaaaagaaa gcggtatatg atattgcaca aagctcgatc gaagcattga cattagacga
     5401 actcgttggc ttagatggga ctagcgtaag taaatttttc gaaaaacgca aaaacgaacc
     5461 attggactat ggctggatcg aaggatcaga tgttttcaaa caagaagtcg cttctcttta
     5521 tcaaaccgtc gatccggaaa acatcttaca aacgaatgga gcgactggtg ctaatctttt
     5581 agctctatac gcgttagtgg aagcaggaga ccatgttgtt tcgatgcttc ctacttatca
     5641 gcaattatat gacatcccca aatctttagg tgccgctgtt gactttgttc atttgaagga
     5701 agaagaagat tggcagtttg atttagaaca gctagaagcc ttagtcaaac ctgaaacaaa
     5761 aatcatctgt ttgaatagtg caaacaatcc tacaggcaca ttgctagatc ggaaaacttt
     5821 agaaaaaatc gcagaaattg ctcgtacagt agatgcatat gtattgatag atgaagtata
     5881 tgcgccttta acggataaag gtgaattctt gtcgatcgta gatgtctatg aaaaaggtat
     5941 agcaaccaat tccctatcca aaacctactc cattccaggt attcgtattg gctggacagc
     6001 tactggacct gagttggcag aagtttttcg aaagtatcga gactacacga tgatttgcgg
     6061 cggagtcctg tcagatgatc ttgctgtaca tgcattgaag aataaagaaa aaatattggc
     6121 gcgaaacaaa aagatcatta cagagaacct tgtgatttta aaacaatggg tagccaatga
     6181 accgaaagtt gaacttgtcg ctcccaatta cgtctctact tctttcatta aattagccat
     6241 cgaagaagat gaccagacat tttgtatcaa tttattagaa gaaacaggag tactattagt
     6301 accaggaagt gcctttgatt taccgaagca tgcccgacta ggttactgtt gtaaaaaaga
     6361 aaccttggaa aagggattat tcctcttatc tgagttcttg aaaaaacagg cctaaacaaa
     6421 aacactgcaa ggacagtctg tgacaactgt tcaagcagtg tttttcattt acgcttattg
     6481 tctatgagaa ggagagtgcc ctgaagctat ggagactcct gaagcttttt cttctgcagt
     6541 ttcttgctga tggatctctt gtgtattgtg tcgtacaagc aagaagttga ccaaaacaag
     6601 aagagaagtc gaaagaaaga cgccacgata accaaatgca ctggaaacac tggagccgat
     6661 cataggccct actacgttac cagttgcttg gaaagattgg ttataactga agatccgtcc
     6721 agcagcttga tgaggcgaat attttgtgat caatgcttgg acagcaggga gcagacaagc
     6781 atcagagata ccaatcaaaa aacgtaaggc agctaattgc caaacattcg tgacgaatgc
     6841 cattgggata taaaccagaa tcgcaaaacc caaaccaatc gcaagtattc tttcactccc
     6901 aatcttgtca cctaagcgac caaatcttgg agcggctatc aaagtggcaa tcccaggtat
     6961 cgaagcaatg attccgctga tcaacgtaac attcccatgt ccatgcagca attggcgaat
     7021 gtacagactg atgattggac taatggaatt gttggatgct tggatgatca tcgttgtgac
     7081 gaacataccg atgacgacgt gtggatattt caagtctttg aaaatctgtt tggcagaaac
     7141 catgttttct tttttcactg ggacaaattt ttcatggacg aaaaacagac tcaacaagaa
     7201 gacgaaaaat aagatgatcc ctgtaatgaa aaaggtaggg cgatagccaa acgctgaagc
     7261 agagatcccg ccaagaagcg gtccaagtaa tgttcctgta acagaaccag ttgctaaggt
     7321 tccaagaact tgaccacttt tttcttttgg cgtcccagtt gcgacaaggg cagtggcatt
     7381 actgatataa ccagaaaaaa tcccttgaag cagacgcaat gcgactaatt gatacacact
     7441 agtcacgcaa cccatcaaag aaatgacgat tgccattcct aatgaagcac gcaacagcat
     7501 caattttctt cctttttggt cggctaattt tccccaccaa ggagagataa gcgtagtcac
     7561 caagaaagta gatgaaaaag tcaatccgct ccaaaagttc aattgtgacg tactataatt
     7621 acctaacgta tctatgtaaa gtgacataaa aggcatgact aaactaaatc cgataccagc
     7681 cataaatgtt ccaaaccata agacaaacaa gttttgttcc caagtttctc ttttcctaaa
     7741 taatttttcc tttattcttg cagccaatta gactccttct ttctgaaaat tttcatctac
     7801 agctctttat tataaactac aatatgtagt ttatcaatga aagtctcttt tttctctttc
     7861 tttttcgtgg taaactgaaa gagtgaagga tttattttat gagaggaaac atggaaatga
     7921 aaaaaagaaa tctgtctcaa gagaaaatca tcgactgctt tcgagaacta gcagaagaga
     7981 tggatgttca gcaaataacc tttcagcatt tagccaaacg cttggatatc aaggctccct
     8041 cgctgtataa ccatttcaaa aatattcgtg atgtcaaaac agcgctaaca gcgaaacttc
     8101 tgcaggaact taatgaccag ctgcgacggg ctcttgtggg aaaaagtggt caggaagcac
     8161 tgcagatcta tgcagaaact tatcgatcct ttgctttttc aaatcaagca gtgtatgagt
     8221 tactgatcag tgtaccgcat accaatgaag ctgttttatt agatggaatc catgaaacga
     8281 atcagatcat tttgcagata tttgaggctt ttcaattaac gaaagaagaa aaagttcatc
     8341 gaagcaggga acttcgtagt atgatccacg gttatctttc cttacgtttt ttaggatact
     8401 ttacaaaaga accaacaatc gatccagaag acagctattt ttggatgatc aataacttta
     8461 tcgctacttt gccttaaaca atcagattca tttgtaacaa ttttaagatg ttcattaaat
     8521 gaccctttta taagagaaaa ttcttgcaac tttcttgaaa agtgactaag atgagtctag
     8581 aaaagctgaa agaaggagca cgagtatgta tcaagtaatt accatgttcg gggacaatga
     8641 gccttggtgg ttttttgaag actgggaaga agatattgaa gaagaagaaa cattcgagtc
     8701 tttcgaagaa gcacgacaag cgtacgaaaa acaatggaac gaaatccatc aaaattatga
     8761 atatatcaat gcaaaaagta actttctcag cgccttttgg aatgacggag atgagcgctg
     8821 gtgtgaagag tgtgacgatg atttacagca atacaaggga cttgcgttgt tgaaaaatca
     8881 tcagcccatc acagtagaaa gcagaaagga attttatgaa acaactaatt ctagcggaaa
     8941 aaccaagcgt tgcgaaagac ctaagcaagg tgcttggtgc taatcaaaag cacaaaaatt
     9001 attatgaagg acccaaggtg atcgttactt gggcccttgg tcatttatta ggattgaaaa
     9061 tgccggaaga tctgaataag gaatggcaga gctggcaaat ggacacgttg ccaatgatcc
     9121 ctaaaaaact aggaatcaaa ccacttccta aaacaggaca tcaattaaaa gcaatcaagc
     9181 agctggctca gcgaaaagat gtctcagaag cagtcattgc gacagatgcc ggaagagaag
     9241 gggaacttgt tgctcgctgg atcttagagt atgtgcattt caataaacca gtgaagcggt
     9301 tatggatctc ttctcaaaca gataaagcaa tcaaaactgg attcaagcaa ctaagacctg
     9361 cgaaagaata tgatgcattg tatgaatctg ctcttgcccg ggcaaaagcg gactggctgg
     9421 taggattgaa tgtgaccaga gccttaactg tgaagtacgc ggataattta tcagcaggcc
     9481 gtgtgcagac accgacacta gcattagtga gagaccaaga aaagaaaatc gagcagttcc
     9541 gtccacaaac gtatttcacg atcacgttgc aagctggaaa agaacaagcc aaaatgcttc
     9601 aaaaaaatca atttgcttta aaaacacaag aagaagcaca aaaacttgtt caattgatca
     9661 gtacaagcaa aggaactgtc acagaagtcc aagaaaaatt aaaaacggaa tctgcgccat
     9721 tgccttatga tttaacagag atccaaagag aagccaatca acgctatggc tattctgcaa
     9781 agaaaacatt aagtcttgta caaagtttgt atgagaccca taagatcgtt acttatccgc
     9841 gtacagacag caaatatctg acaaatgaca tgaaaagtac gatgaaagaa cgattgcaag
     9901 cagtcagcga ctttgctcct gaggtcaagc agtatttgaa aaacggagcc atcgtccaac
     9961 agacaaaagt attccaagac agtaaagtga ccgatcacca tgcacttttg ccaactgaaa
    10021 acagagcacg ttatgaaaaa ttaagtaatg aagagcgaaa aatctatcaa atgattgtct
    10081 ctcgtttttt aggactgttt gctcaacccc ataaagtcag ccaaacaaaa gttacggttg
    10141 aatttggtaa agaacgattc atttttcggc aaaatcgtgt cgtccaagca ggatggaaag
    10201 gcgaaactga aactgagacg gaaacagtca aatgggaaaa aggaatgaga atcaatcccg
    10261 atttcgccat caaaaaagaa ctatctgcac cgcctaaacc attgtcagaa gcaagcttgc
    10321 ttggtcaaat ggaaaaatac agtctgggaa ccccagctac gcgtgcagag attatcgaga
    10381 aactaatcaa atctgaactg atggaacgta cccctcaagg actgcaagta tctcctaaag
    10441 gtaagcaatt attagtattg gtaaatccct ctttagtaac accagatttg acagaaagct
    10501 gggaaaaaga ccttgaagct attgctgcag gaaagaaaca agcaggaacc ttcttgaatg
    10561 gaatcgaaaa agaaacaaaa cgcttggtga atgagatcaa atcaagcaaa caagaatatc
    10621 aagatttctc gattacccaa aagaaatgcc cagaatgtgg ggccaatctg cgcgagaaaa
    10681 acacacgtga cggcaaaatc tatgtctgca ccaattcaga ttgcaactac cgcagaagaa
    10741 aagatccaaa agtctctaat catcgatgcc cgcaatgtca ccggaaaatg gaaatcatcg
    10801 aaggtaaaaa cggtgcttat ttccgttgta aatacgatgg aacgacagaa aaattgcttg
    10861 ataaaaaaga gcagaagaag aaaatgacca aacatgaaga acgacgcttg ctgaaaaaat
    10921 atgcacaagc agaagaacca gaagaaagcc cattagcagc agcactaaaa gcagcaatga
    10981 agaagtaata agatatgaaa gaggttgtga tagaagtggt cagcttcaag aaataagaag
    11041 gaatttacga aaattgcttc tcaaatttt
//
LOCUS       DAZULY010000087        10750 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000087 DAZULY010000000
VERSION     DAZULY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.088"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(25..162)
                     /locus_tag="ACG81A_001461"
     CDS             complement(25..162)
                     /locus_tag="ACG81A_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFS5014832.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /gene="mobT"
                     /locus_tag="ACG81A_001462"
     CDS             complement(159..1343)
                     /gene="mobT"
                     /locus_tag="ACG81A_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS5014833.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="ACG81A_001463"
     CDS             complement(1599..1853)
                     /locus_tag="ACG81A_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014834.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="ACG81A_001464"
     CDS             complement(1878..3020)
                     /locus_tag="ACG81A_001464"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFS5014835.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="ACG81A_001465"
     CDS             complement(3080..4432)
                     /locus_tag="ACG81A_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS5014836.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="ACG81A_001466"
     CDS             complement(4503..4847)
                     /locus_tag="ACG81A_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS5014837.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="ACG81A_001467"
     CDS             complement(4857..5231)
                     /locus_tag="ACG81A_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5014838.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="ACG81A_001468"
     CDS             complement(5244..5558)
                     /locus_tag="ACG81A_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5014839.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="ACG81A_001469"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="ACG81A_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="ACG81A_001470"
     CDS             complement(8976..9638)
                     /locus_tag="ACG81A_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014840.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="ACG81A_001471"
     CDS             10255..10485
                     /locus_tag="ACG81A_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014841.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            10491..>10750
                     /locus_tag="ACG81A_001472"
     CDS             10491..>10750
                     /locus_tag="ACG81A_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS5014842.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDY"
BASE COUNT         2966 a         2377 c         1826 g         3581 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
    10741 atgactatat
//
LOCUS       DAZULY010000088        10624 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000088 DAZULY010000000
VERSION     DAZULY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.089"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1239
                     /gene="lysA"
                     /locus_tag="ACG81A_001473"
     CDS             <1..1239
                     /gene="lysA"
                     /locus_tag="ACG81A_001473"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFS5014843.1"
                     /translation="GVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSKRFDTKVL
                     YASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKTPEELIYA
                     LENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTAHIDSKFG
                     MSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYLTDFDQPL
                     SLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPGRSIVGEA
                     GTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKTEKVTIAG
                     KMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFVNNGQSRL
                     IVRRQAFSDLLRGEVSYDPS"
     gene            1226..2026
                     /gene="dapF"
                     /locus_tag="ACG81A_001474"
     CDS             1226..2026
                     /gene="dapF"
                     /locus_tag="ACG81A_001474"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFS5014844.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2029..2910
                     /gene="dapA"
                     /locus_tag="ACG81A_001475"
     CDS             2029..2910
                     /gene="dapA"
                     /locus_tag="ACG81A_001475"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFS5014845.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            2907..3623
                     /gene="dapB"
                     /locus_tag="ACG81A_001476"
     CDS             2907..3623
                     /gene="dapB"
                     /locus_tag="ACG81A_001476"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFS5014846.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3629..4324
                     /gene="dapD"
                     /locus_tag="ACG81A_001477"
     CDS             3629..4324
                     /gene="dapD"
                     /locus_tag="ACG81A_001477"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFS5014847.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4340..5341
                     /locus_tag="ACG81A_001478"
     CDS             4340..5341
                     /locus_tag="ACG81A_001478"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFS5014848.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5338..6699
                     /locus_tag="ACG81A_001479"
     CDS             5338..6699
                     /locus_tag="ACG81A_001479"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFS5014849.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6659..7810
                     /locus_tag="ACG81A_001480"
     CDS             6659..7810
                     /locus_tag="ACG81A_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS5014850.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7785..8954
                     /locus_tag="ACG81A_001481"
     CDS             7785..8954
                     /locus_tag="ACG81A_001481"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS5014851.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9129..10508
                     /locus_tag="ACG81A_001482"
     CDS             9129..10508
                     /locus_tag="ACG81A_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5014852.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         3507 a         1871 c         2397 g         2849 t
ORIGIN      
        1 ggagtcacag caagcacatt ggcagaaaag tataagacac ctctgtatgt gtatgatgaa
       61 gaaaagatga gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa acgttttgat
      121 acaaaagtgc tttacgcctc taaagcattc caaacgattg agatgctgca tttagttcgt
      181 gaatatggat tcgggctaga tgttgtcagt ggtggggaaa tctataccgc tttgcaagct
      241 gattttccta gagatactat ctacttccat ggtaacaaca agacgccaga ggaactgatc
      301 tatgcacttg aaaatgattt gttgcatatt gttgccgata atttgatgga agttgaatta
      361 ttagcagaat taagccaacg ctatttaaaa aaaatctgta tcatgcttcg gttgaatgtc
      421 ggagtagaag cacatactca tgaatatatc gtcactgcgc atattgattc aaaattcggc
      481 atgagctatg aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga aagcagctat
      541 ctagagttag aaggatttca tgctcatatc gggtcacaaa tttttgatgt tactgcttgg
      601 ttagctgaaa tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc tctttccttg
      661 aatcttggcg gagggtttgg gattcgttat acacaaaatg atcagccgat cccagtcgaa
      721 gaggcactaa aaaaactgat tgaatatacg gaagaagctt tggaaaaacg aaaactgacc
      781 attcgtcaat tactgatcga accgggtaga agtattgttg gagaagctgg tacaacacta
      841 tacacgattg gtttcatcaa aaagacacca cataaaaaat attactttgt agatggcggg
      901 atgacagata atatccgtcc agctctgtat caggcagaat acgattgtga tttagcagca
      961 aaactgtcgg aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc
     1021 gatgtggtaa ttaaagaagc ttatttgccg aaagcagaat cgggtgatct tttagctgtt
     1081 tattccacgg gagcttatgg atactcgatg agcagcaact acaatcgagc gacacgacca
     1141 gcagttgttt ttgtcaataa tggccaatca agactgattg ttcgcagaca agcattttct
     1201 gatctactaa ggggggaagt ttcctatgat ccttcataaa tataatggct gcggaaatga
     1261 ttttatctta ttagattacg cagaagacac agattatagt caattagccg cttctttatg
     1321 cacaaaagaa aaatatgata cagacggatt gatcgccgta aaaatcgatc cgttggaaat
     1381 gatctattac aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt
     1441 tgctcgatac gtaaatgatc agaacatgat gaatgaacag gaattcaacg tagagacatt
     1501 agcaggcaca agaaaaatta cggtgttgga tccctcacct ttttattgca gcgtagatat
     1561 gggagtgcca gatgattctc cggaaaaaat caaagctaat caaacttatc caataaaaaa
     1621 ccaggaaata gagatcaatg gtgaaaaagt aacgatcact agtttatttt taggaacgat
     1681 tcatacagtt gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact
     1741 gatctgtcat catcctttgt tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa
     1801 tgaacaagaa ttgatcgtcc gtacctatga aagaggagta ggttggacac ttgcttgcgg
     1861 tacaggttgc tgtgcctctt atgtggttgc taaggaaact gggaaaattt cagcagatca
     1921 ggtagttgtt cacttggaac aaggagactt gatgatctct ggtgaggatg cgatacaaat
     1981 ggctggccca gcagttcatg aatggacaag aatattggag gaatgaagat gataaaaggt
     2041 tcgatcgtgg cgctgatcac gccgatgaat gaagagggat cagtagatta tgctggtttg
     2101 gaaaaattga tccaattcca tttagatgaa caaacagatg gattattagt tttaggtaca
     2161 acaggagaat cttctacgct cacacaatct gaggaggagc agatccttca actgacagta
     2221 aagaaagtgg ctggccgtgt gccggtgatt gcaggagctg gaacgaataa tacgaaagaa
     2281 acgattgaaa aagcgaagca ttttgcttct ttgggggcag acgcactttt ggtcatcacc
     2341 ccttactaca acaaaacaag tgatgcagga ctagctgctc attttacagc aattgcagaa
     2401 gcctcaccga ttccgcttat tttgtataat gttccttcta gaacaggcat gtcgattcca
     2461 atccatgtat tagtaaattt agcagaacat cctaatatca ttggtttgaa agaagcctca
     2521 ggagatatgg cttatgtgat ggatgccgct cggttgattg gagaagaatt cttcctttac
     2581 agtggaaatg atgatttgat cctaccagta atgagtgtag gaggaagcgg tgtaataagt
     2641 gtatgggcga atatccaacc taaaatagtc catgaattag taaaagatac gcaggacgga
     2701 agatggcagc aagcaaaaga aaagcaatta aatgcacttg aactgatcca tgccttgttc
     2761 agtgagacca atcctattcc tgtcaaagca gccatgtcat tattagactt accttccgga
     2821 cctctacgcc tgcctttagt ttctctttca aaagagaaaa agaaacaatt agctcagctt
     2881 ttgttaaaag aaaggacggt tgaaaaatga aaatcggact gattggcagt ggacagatgg
     2941 gaaatagagt cgaagaagtt gcacaagaaa gaggagatac ggtccttctt ttacggacgg
     3001 cacctaaaga agcaactacg cttgctttta cggaactgcc agaactgctt attgattttt
     3061 ctcatccaga taatttagag aaaatccttt cgtatagctt atcatatcaa cttccgttag
     3121 tcattggcac gacaggttat acacaatcac agttccaaga aataaaagaa catgccaaac
     3181 atgtacctgt gatgtatagt gctaattttt cgttcggcat catggtgatg aatcaactaa
     3241 tcaagcaagc agctgcaatg ttgcaaggct ggcagatcga actgattgaa aaacatcata
     3301 gtcgtaaaaa agatgcacca tctggtactg caaatatgct gcttcaaaca atccaagaag
     3361 tgcaaaagct gcagcccgta tacaactggc aagggaaaaa aagagaagaa aaccaaattg
     3421 gtgtacacag tattcgtgca ggttctttac ctggtgaaca tgatgtattg tttgctgcta
     3481 ctgatgaaat atttacgatc aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg
     3541 cagtagaagc agcggactgg ttgaaagatc agtcaccagg ttattacaaa ttagaagatt
     3601 tattaatgga caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc gctacattca
     3661 aacggcatct aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt
     3721 taaagaaagt aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg actatgagac
     3781 gattgctccg gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc
     3841 tgcaagatac agcgcagttc ctttgctaga tattaaaaaa attaatgctc gtatcgaacc
     3901 aggtgctatt atcagagatc aagtgtcaat cggcaacaat gccgtgatca tgatgggagc
     3961 gattatcaat attggtgcag taatcggtga aaacacgatg attgatatgg gtgctgtctt
     4021 aggtggacga gcgacagtgg gcaaaaactg ccatattggt gcaggagctg tcttagcagg
     4081 tgtgatcgaa ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc
     4141 gaatgcagtg attgttgaag gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc
     4201 agtggtttta gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt
     4261 aaaaataagt gatgatcaga caaaagaaaa tacagcattg attgctgcgt tacgcgaatt
     4321 atagaggaga tggagaaaaa tgcgcgaaat agatacctgt attgtggggg caaccggatt
     4381 agtcggccaa acgatgctca aagtgttaga agaatatgat tttccagtag gacgattgaa
     4441 attattagct tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa agaaacacac
     4501 tgtacaggaa ttgacagaaa cttcatttga aggatatgat ttagcgctat tttctgcagg
     4561 tgcttctgta tccaaagagt ttgcgccaat cgcacgaagt cagggactat ttgtcataga
     4621 taattcctca gcatggcggg aagattcgga tattccatta atcgttccag aggtcaatct
     4681 ggaagactat acattgaatc ggctgattgc aaatccaaat tgttcaacca ttcaagcggt
     4741 acttccgctt aaagctttac aagaagcctt tggtatccag cgagtcaatt attcgaccta
     4801 tcaagcagtt tctggttccg ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga
     4861 cgaggaacca gttttgtatc cttataatat tagccaaacg gtgattccgg aaatcgatca
     4921 gcctttggaa aatgggtata caaaagaaga acagaaaatg ataaatgaga cccgtaagat
     4981 tctgcatcag cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg
     5041 acatggagtc tctgtggcag ttcaactgca aaaagagttt acgacggaag aaattagaga
     5101 gtgtctcagc cagtttccag ggattcaatt agaagatgat ctagcagcac ataaatatcc
     5161 gacgagcatt cttgctagag gaacagatat ggtttatgtt gggaggatca gaaaagacac
     5221 ttctttagag aacggattgt tgttctatac gactgccgat aacatacgca aaggagccgc
     5281 tgcgaatgcc gtacagattg ctgcagcttt aatgcagaaa atagcggagg tggcaagatg
     5341 agtaatacgc tgaaagtagc gaaattcggc ggaacatcga tgtctcatga agagcaattc
     5401 aaaaaagtcc gtacaattgt tctttcagat cccaatcgcc aagtgatcgt tgtttctgct
     5461 ttaggcaaac gtacgtcaga tgatgacaaa gtaacagact tactttattt gatttatgct
     5521 taccttcagc atcatgttga ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt
     5581 caagtaagag atgttcttgc cctgcagtta cctattgaag atgaactagc acgcatacag
     5641 aaacaactgg aagacgaaaa gataactgaa gattatttag taagtagagg tgaatatctg
     5701 actgctcaac tgatggctga gtatcttggt taccagttta ttgacgcaaa agacctgatt
     5761 ttctttgact atgatgggaa aatcgatcag gaaaaaacag cacaggaaat cacaaaagca
     5821 tttaaaagat atccgaaaat ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa
     5881 gaaaaattat tgggccgtgg gggaagcgac attaccggcg cgatactggc gaagatgttg
     5941 caagcggaaa gttatgagaa ttggacggat gtttcaggaa tcatgatggc tgatccgcga
     6001 atcatcgatc atcctaaaat catcgaggaa atctctttta gagaactgag agagatggct
     6061 tatatggggg ctaacgttct tcatgaagca gctgttttcc cagttcaaga ggctgatatc
     6121 cccatccaaa taaaaaatac caatgatcct ttcgctcaag ggacaaaaat atcgaatcac
     6181 gaaatagaaa aggaaaatgg actgacagga atagctggac gcaaggattt tctttccatc
     6241 actttattca aacgtcatat gtccgatgaa atcgggttta tctggaaagc aatgagcatt
     6301 tttgctaaac atgagatatc aatcgaacat attccttcag ggatcgacaa tatcggagtg
     6361 atcgtctcag cagaagcgat tgccgataag ttatttctca tcacgaaaga attaaaagaa
     6421 acacttggcg tggaagaaat cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg
     6481 ggtccgcata aagaattgat tggattatct gggaaagtgt tatcgatatt gaacaaatta
     6541 gaaattcgca catcgatctt atcacaagga gcacaggagt tgaatctgat catcggtgta
     6601 ccgaataatc aatatgaaac agtagtgaag ggtatctatg aagggatggt gaatacggat
     6661 gctgaaagaa tatcgaatgg cactccatca aattcctgaa atcgggtttc atgaattcaa
     6721 gacaaaaaaa tatttatatg aacaagtaaa aaacagcggg gccgttatcc atgaaatcgg
     6781 cgaaacagga ctactccttt attttgataa tcagcaagaa gaaacgattg cttttcgaac
     6841 agatatcgat gcactgccag tcaaagaaga aacgaatctc tcttttgctt ctaagcatga
     6901 aggttatatg catgcatgtg gtcatgacgg acatatggcg atgcttttag ggttgactga
     6961 ctttataact aaacacattg aggatcttat ttataatgta gtactgatct atcagccatc
     7021 agaagaaatt tccggtggcg cacaaagcgt tattcagtca ggattgttgg ataaatatga
     7081 agtacaagca atatttggct ttcatctatg gcctggacta gctaagggag aagttttctc
     7141 tcgaccagga cctttgatgg cacaaagcag tgaaacagac attatcatta aaggccagtc
     7201 tgcacatatt gcttctagtg aaaaaggaat cgatagtctg gaggcagctg tacgattttt
     7261 gaagaatgtt tatgattttg accggcaatt accggcagag tggaatcatc taatgaaatt
     7321 tggccaaatc caaggtggaa cgatccgcaa tgttttagct ggagaagtta ttgtatccgg
     7381 aagtatccgt tcttacagtt cagatatgca aaattatttg aagaggggat tagaccattt
     7441 agcagaacag tttcactcta cagctactat ttcttttcgc tataatgacg gctatccagc
     7501 tgtaatcaac catagaggct tatatgcagc actgttagat tcaaacaaac tacatgaatt
     7561 agatgctcca gttcttcaag ccgaagattt tggcatatat acgcaacaat atccatgtgt
     7621 atttttcttt ttaggtgtcg gtgatacccc tgctttacat gatccgacgt tcgattttga
     7681 tatggatgtt ttagaaaatg gtgtagaatg gtacaaaacg attcttttta cagaaaatct
     7741 tcttgtaaaa atcgatagca gttcaaaaga ggagcgagta aataatgatg aatttatcca
     7801 atcgatttag tcctaggctt tctagaatag aagtatcaaa aatacgacaa ttcgatcagc
     7861 aaatatcgtc gataccagat gtgatcaaac tcaccctggg agaaccggat tttccaactc
     7921 ctgaacatgt caagcaagca ggaatagcag cgattgaaga agacttttct cactataccg
     7981 gaatgagagg attagaggaa ctgcgggaag cggcttgtat atttcagcaa cagcgttatg
     8041 gattaactta tgatccgcaa acggaagttt tgacgacagt tggtgcaacg gaagcgattg
     8101 cctctgcgtt gttatctgtt ttggaagaag gagacaaggt tcttattcca gcgcctgctt
     8161 attcaggata tcagccatta gtggaactag ctggtgctga attgatcccg atagatactt
     8221 ctgacacagg gtttgtctgt cagccggaac aatttgaacg agcatttgaa caatatggat
     8281 cagctgtcaa agcagtcatt ttaaactatc ctaacaatcc tactgggacg acattgtctg
     8341 caaaacaatt gaaagcaatc gctgaggttt taaaaaaata tccggtattt gttataagtg
     8401 atgaagtata tgctgaattg acttatagtg gaacgcatat gtccatagct acttatcttc
     8461 cagaacaaac aatcgtgata agcgggttat ccaagtcaca tgcgatgact ggttggcgag
     8521 tgggctttat ttttgctcaa aaagccctta ttgacgaact aattaaagtc catcaatatc
     8581 tggtgactgc cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg
     8641 tgaaagatag cgagaatatg aaagagcaat atcaaacacg aagagactat ctggtccagc
     8701 aattggcgcc tttaggattt gaagtgaccc agccaaacgg tgcattttac ttgttttgca
     8761 aactcccagc tgccattcaa actgactctt ggcagttttg tgtagaatta gcggaacagg
     8821 caaaagttgc gtgtatccca ggaattgcat ttggcccaga aggagaaggt tatatccgga
     8881 tttcttatgc tagcagcatg gaaaaattac atgaggcctg caaacggatc aaagactttt
     8941 taacagaaca gtgattcata gattttatca aaaaaacaat cgttaatttt gcggtatatt
     9001 ctgcaaggag acgattgttt tttaaattta gttcaaatga acgcgtttct tttgattcat
     9061 aactaatgta atgttaagtt taaattgttt agaatgattt taaaagaata ttcaaggagg
     9121 tttcatcaat ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga gggcatcaat
     9181 cgatcttgga actattgaca cgatacggtg aaaatgtaga tatcacgcta tttgaagcag
     9241 gagactatat ttccttcatg tcatgtggta tggagctgta tttagaagac aaggtaacag
     9301 atgtaaatga tgtacgtaat ttcagaccag aaaactttcc gcaatccaat gttcatattt
     9361 taaataatca cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg gttactagcg
     9421 catcagataa cgaaacaaaa gaatatcctt atgacaaatt gattttgagt tctggtgtca
     9481 aaccaaacaa tctgccagta ccaggagcag atctagaaaa tgtctacttg atgcgtggat
     9541 acaattgggc aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg
     9601 tcgtaggtgc gggttacatt gggatcgaag cagcagaagc aagccgaaaa gctgggaaag
     9661 aagtcgtatt gcttgatgtg atcgatagac ctttaggcac ttatttagat gctgagatga
     9721 cagatatttt agaacaacac ctaaatgaaa acggtattga agttttgaca aatgcaaaaa
     9781 tcgaagcttt tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc
     9841 cagcagatct agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga
     9901 tcgtcgactt agatgaacgt ggctggatcc aaacaaatga atacttgcaa acgaatcttc
     9961 cagatgtgta tgcagtagga gatgctacac ttgcttattc tatcccagca cgcaaaaaaa
    10021 tgccgatcgc tttagcgact gttgcacgta gagaagcacg ttatgtagta aaacatcttt
    10081 ttgaagaagt accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct
    10141 ttgactatca ttttgcagca agcggattga atagctttac agcagcaaaa gcaggagtag
    10201 atgtacgagt gtcttattat gaagatacgt tacgaccaaa atatgtacca gaagaacaag
    10261 gaaatccaaa agtgtccatc caattattct cggatccaat gacccatcaa ttgctaggag
    10321 gagcagtatt atctacctat gatgttacag gtcaaggaaa tgtattggca ctagcgatcc
    10381 aaaacaaact gactttagaa gaactagcag aagctgactt cttcttccag ccaggctttg
    10441 accgacaatg gagcatgttg aatctagccg ctcaacaagc gttaggagaa gaaccatttg
    10501 tagaataaga aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta
    10561 ataagaacag ccaagtaaaa acagcttctt atgtttttaa cttggctgga cttattaccg
    10621 caga
//
LOCUS       DAZULY010000089        10540 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000089 DAZULY010000000
VERSION     DAZULY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.090"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..2132
                     /locus_tag="ACG81A_001483"
     CDS             72..2132
                     /locus_tag="ACG81A_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFS5014853.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2215..2784
                     /locus_tag="ACG81A_001484"
     CDS             2215..2784
                     /locus_tag="ACG81A_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5014854.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2959..3765)
                     /locus_tag="ACG81A_001485"
     CDS             complement(2959..3765)
                     /locus_tag="ACG81A_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014855.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3903..5417
                     /locus_tag="ACG81A_001486"
     CDS             3903..5417
                     /locus_tag="ACG81A_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFS5014856.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5820..6803)
                     /locus_tag="ACG81A_001487"
     CDS             complement(5820..6803)
                     /locus_tag="ACG81A_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFS5014857.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6808..7455)
                     /locus_tag="ACG81A_001488"
     CDS             complement(6808..7455)
                     /locus_tag="ACG81A_001488"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFS5014858.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7540..8172)
                     /locus_tag="ACG81A_001489"
     CDS             complement(7540..8172)
                     /locus_tag="ACG81A_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5014859.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8188..8739)
                     /gene="coaC"
                     /locus_tag="ACG81A_001490"
     CDS             complement(8188..8739)
                     /gene="coaC"
                     /locus_tag="ACG81A_001490"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFS5014860.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8736..9473)
                     /locus_tag="ACG81A_001491"
     CDS             complement(8736..9473)
                     /locus_tag="ACG81A_001491"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFS5014861.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9470..10459)
                     /locus_tag="ACG81A_001492"
     CDS             complement(9470..10459)
                     /locus_tag="ACG81A_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS5014862.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
BASE COUNT         3392 a         1993 c         2107 g         3048 t
ORIGIN      
        1 ttatataatg aaatcatctt atgaaaatta ctacttcatg tttagacaag agtagggagg
       61 actaagaata aatgtgttca aaaagacgtt caaaacaact aaataaacac gcttgtcgtt
      121 tagataagga acggtcgtgt tctgaaaaac actacgttaa gtttatgatg cgcacgatgc
      181 tgatcagttt atctgtaggt gtctatatgc atgtacccgt tcaggcaacg acacaaacca
      241 tcaaggtcgt acgtacctct gtttctgaga ccactgattc ccctatcact attactcata
      301 ctttcactga tgcaaatgga aaacagctac ttcctgcatt gacttcctat aataaagtca
      361 gttatcctcc aacgataaac cactactggt taattcctaa caaaacatta tggacatcgg
      421 aaaccggaag cagtgtgccg ctttctatct tttccgatat gactgatcct acaaaaggtg
      481 atcagttaaa acagatcact gatattttga atcatgcttt gacagatata aaggacggtg
      541 gaagcgcaca agtttcttat gtatatgaaa aagattattc acagtttgaa agaaagaaaa
      601 tcgatgttct ggcagggaca aaagtttcga tatctgatct ggtagaaagg ctgaagaacg
      661 cagatggaac acaaggagat atgtccaaag taatttcaaa caatgggaat atcgtggata
      721 cgactaatac aggaacatct actatttcct tgtcctattt tgatccaatt gctttaaaaa
      781 caatgactgc atctgctgtg atccatgttc gtgctgatca aacagctatc aaaggaaaag
      841 atcttcactt aagaacagga gagagttacg cccctaaaga tcttgttgaa tatgtaacaa
      901 aaagcgatgg aacttcagga aataaagaag aaatcaaaat gaacgggaca gtagatacta
      961 caactccggg agaatatccc gttgtattaa cttatataga ctcgaaaaca tcaaatgtgg
     1021 cagaaacaac agctgtagtt tatgtagaag catctgctgc ttcatcgact gtcatcactc
     1081 attttataga tagtcaaaca aataaagaac ttcaagcaga aacacatatg agtagtgtaa
     1141 atcgtattcc tgatttgcgc ctacctgctg aattttctga tcgagtgcca aatatagatt
     1201 tatcgactta tgaacaaaat gggaaagtca cttctatttc tgattggatg caagcaaacg
     1261 gattagacag aaaaagtcac tggtcggaga tccttgaagt acttcactca gaactgatga
     1321 ataataggaa gcaatcagga ggggaaatta cctttactta tgtttatata ccagacaatc
     1381 gttcattaga aggtcatgat cttattgttc atccaaacga aaaaataaac aaagaagagt
     1441 tgatccaatc agcaacaaat tccttaggta aacccgtacc gttaaaggat gtatcggtta
     1501 caatcaatga agtaccattg gaggatggtt atttgaccaa acaaacaggt gattttacga
     1561 ttatgtatag ttatcatgac ccgtattcac tccaagaact ccatgctcag tcactattac
     1621 atgttttacc attaaacaaa cagatagatc aaaaagaaca aaaaagacga gaagaaagct
     1681 tgtcagaaaa aaaacaacaa atggctcatg cagtttctcc aacagggaaa gtaaaacaaa
     1741 caagaagcct agagttagca agaatatcta gaaaaatctc agaatctaat aaaacgataa
     1801 ggcaagaagc tcatttggcc ccacaagttt ctggattgga aaatgaaatt ttagagccta
     1861 aaataaaaca acaatcagaa aaagaatttg ttgctaaaca gtcggaagat cagaaggaat
     1921 cagatgacca gaaggaaacg aagaaaaaac gcttgtcaac tattgattcc accgatgagc
     1981 atctgcagcc gcaagtacca cttcctagtc cgggaacacc cgcacctggt ggaggtattc
     2041 cttcttctgc tttaggagat ttcatgcgag gtatggcagg aacatgggta tacgctaatc
     2101 gaaatgaatt tgacgcagat gactggttct gatttatatc tctatctgtt atagtcaaat
     2161 aagtagctga gtcatatgtt atttggcata atggagacga ggagggatag aaaaatgtta
     2221 gataaaacgt taccttacgc agaaatctgg atgacaagac cacttcaaca aaatgttcca
     2281 atcacgcctt tagcggaagg ttttgaattt cgattttatc aaaaagaaga tgaatctgct
     2341 tgggcggaga ttgagacttc cgttggggaa tttcaaacga cagatgaagc acttgagtat
     2401 tttcaacaaa actttgctcc ttatcctgaa gaacttgaaa gaaggatgtt ttttgtagag
     2461 acaaaaaaag gagaaaaagt agcaacctgt accgcttggc gaaaaaaatc aagagagcaa
     2521 gtgtatccgg tttttcattg gttagcagtg aaaccagatt accaaggaaa agggctcgca
     2581 aaagcattga ctgccagagt cttgcaagaa tttcctaccc tccatacgga cggaccaatc
     2641 tatcttcaca cacagacatg gagccatcag gcgattgctc tttataaaaa aatgggtttt
     2701 acttttattg ctgaaaatct agacggcaca cctaatccag attatgaaaa agttatgcag
     2761 gttttaagta aattagataa ataacgaaat ggtgaaataa gtcctcagta ttgggataaa
     2821 aaacgaaata gtcattcctc atatcttact tttttgagtt aatactatcg gataactaat
     2881 ggcatgccat acattcaaaa aagaaggggc agtgacaaaa gtattgtcac tgccccttct
     2941 cttgtttttt agcatgtttt atcgtttgat ttttttagga ataaaattct tgatgaacgt
     3001 aaatttttgg taatcgtttc gactatagag gtaaccaaat acactgaaaa caactgctcc
     3061 aataaaatta acaaataaat ccttcatagt atccatgatt cccacgtcta agtaaccatt
     3121 ttggatgatt gttttatctg ttttaccatc tgcattttga ctttcaatga ttgtccgagt
     3181 gatatctgga acatgataga cagtgtttcc gtctttactt aactccacac tattgatttg
     3241 ctggatgatc gtatcttttt gcatatcgaa ttgcagtaat ttatctgctg aaaattccac
     3301 aaactcccac ataacaccaa ctgtcatcga aaaacagaag gaaacaactg ctagtaataa
     3361 aggagtcaag ttcatggttt ttgtattttt attgagcaaa taaacaaggg aaaagccgac
     3421 acccccagct aaaaatccgc tgaatgcatg tagaaaagta tcccacattg aaaaatagcc
     3481 ataaaaatca cttaactctc ctaaaactgt tgaactaaaa ataaaagata acaaaatgat
     3541 ttctagcata tttggaatct gtatctttaa tatcttttcc agaaaataag ggatttcaaa
     3601 taaaattaat gtcagtaata aaagcattgt atttccccag tgttggttcc agacctgatc
     3661 cactagcata ataacgacga tgatacgtaa taaaatatag attccgtttg tgattctacc
     3721 tgcagttttt gtactgttcg taaaaaaggt tttcaaatga ttcataaaat aactccttca
     3781 tgtatatcct taattatact aagaagcgtg tgtttaccaa agctatatga cttaaagaaa
     3841 aacagagttg atattgtgaa aaaaatcact taatatagta taaggagaaa agggggatac
     3901 aaatgaaaaa attagttgct ggtttattaa cagccgttgc acttcttgct atgagcgcat
     3961 gcggcgcaga tcagaaaaat acagcgcaga caagttctag tacgaaggaa tctgccgaag
     4021 tatcatcagg cgcttctgaa caagtatata cagatccaaa agaaatgaaa gaatcttatg
     4081 atgtcgtgat tattggagct ggtggtgcag ggatggcagc agctatcgaa gcaaaagatg
     4141 ctgggttgaa tccagtaatt ttggaaaaga tgcctgttgc cggaggaaac acatctaaat
     4201 catcaagcgg tatgaatgct tcagaaacaa aatttcaaaa agaagaagga atcaaagact
     4261 cgaatgatgc cttctatgaa gaaacgctaa aaggtggtgg aggaacgaat gataaagaaa
     4321 tgcttcgata ctttgtcgat cactctgctg atgcggtcga ttggctagat caaaacggta
     4381 tcaaactgga caacttaacg atcactggag ggatgagtga aaagaggaca catcgtccgt
     4441 ctgatggctc ggctattggt ggatacttag tggaggggtt attgaataac gtccatgagc
     4501 gggaaatccc tatttttgtc aacgcagatg tgacagacat taagaaaaat ggggaaacgg
     4561 tcaatgaagt gactgtacat gttcaaggag aagaaccaaa agaggtcacc ggaaaagcaa
     4621 tcgtagttac cacaggcggt tttggtgcca gcaaagaatt tattgaaaaa tatcgtcccg
     4681 atttagctga ttatattacg acaaatcaag aaggatcaac tggagatggt atcaccatga
     4741 ttgaaaaatt aggtggacaa accgttgata tggataaaat acaaattcat ccaactgtgc
     4801 agcaggatga gggggtcttg attggtgagg ttgttcgcgg agaaggagca atcttggtcg
     4861 atcaagaggg gaaaagattt gtcaatgaaa tggatacaag ggataaagta tctgcagcaa
     4921 ttacagcatt gcctgaaaaa tcagcttatt tgatttttga ccaaggcgtg cgtgatcgtg
     4981 ccacagctat tgaattttat gaccaaaaag gatatgtcac aactggaaag acaatggaag
     5041 aattagcaga aaaaatcgat ttaccaaaag aaacactagc aaaaacagtt gatggatgga
     5101 atacggatgt tgcggagaaa aaagatgctc aattcaaccg tgccacagcc atggaacatg
     5161 acttatcgtc tccgaattat tatgcgatca aaatcgcacc aggaatccat tacacgatgg
     5221 gcggtgtgaa aatcaatacg aacactgaag tattagataa agaaaaccag ccaattaggg
     5281 ggttatatgc tgctggtgaa gtcacaggtg gcttacatgg aaacaaccgg atcggtggaa
     5341 attcagttgc ggagatcatt atttttgggc gacaagcggg catacaagca gctcgatttg
     5401 cttcggaaaa tgaatgaaat agaacgtaaa taaaaagagg ttgtgaaatc agcgttttga
     5461 gctaataccg agggtcagct gattgaacac tgtttagtga agaggttgtg aaaaagctgc
     5521 cctgcatcaa gtaataagaa cagccaagta aaaacagctt cttatgtttt taacttggtt
     5581 ggacttatta ccgcagatgc aagcttttga acaccgttta ttcggaaaaa gagcgtggga
     5641 caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcagaag
     5701 aaattccttc ggaaataagc tgaaattcac aaaaatttga aaagcaattt tcgtgaattt
     5761 cttcttattt cttggagtta aacgcttctg tcccagcctc ttttttgatc agcatgattt
     5821 tattcagtta tcttcaaaat gatttttccg atgtttttgt ttgcttccat atattcatgc
     5881 gcttgtttga ctgcgctgaa ttggaatgta cggtcaatga ttggccgaat cttgccattt
     5941 tctagaaaag gcccagcttt ttgcataaaa gtttgtgcga gctctgcttt atactgatca
     6001 cttctaggtg tcaataatgt accaagcagt tggacacgtt tcccaatcaa tacaccaagg
     6061 tctatctcag gcacgatggt cccacctagc ataccaatca agacccaacg tccatcaacg
     6121 gctatagaag ctaaattctt ttcccagtaa gaagcgccga tgaaatccaa aataacatcg
     6181 actccccgac cttcagtgat ttttcgtacc tcttcttgaa agtcttgttt tttatagttg
     6241 atcaattcat ctgcaccaag ttctttacaa aaggcgagtt tttcttctga tcctgcagta
     6301 acgatgattt tcgcgtgaga aagctgtcta gctaattgga tcgctgctgt tcccacacca
     6361 cttgctcctg catggatcaa gacagtttcg ccttcttgta attttccaag ccagtaaagt
     6421 gtttgataag cagtcaaaaa aacttctgag attccagcag cagaaacata gtctagcgta
     6481 tcagggagaa ggattgcacg atcggcaggc atcacagcat attcggcata accacctaga
     6541 ttgactaatc cgtatacttt tgtcccagga gtaaaagtgg agatatcact tttattctca
     6601 actacctctc cagctacttc gactcccaaa atagggtagg gaggtttcaa agagactgat
     6661 tctcttttca taatatctgt tcgattgaca gctgctgtat ggactttgat cagtaattcg
     6721 ccttgttttg gtttaggaac aggcacttca atttgttcta atacatctac agaaccagga
     6781 tgcttgattg aaatggcacg catggatcta ttcctccttt gcccattctt ggaatgctgt
     6841 attcaactct ttgctacctt cagaaagtac tagagtcgta cgagaaatat ccgtttcttc
     6901 cactagagcg gcaagtacag cagcaactgt agagcgagtg atcgtttttg atgccttctc
     6961 attaggctta gttaattgaa tggaagtaac ttcttcatcg ttagtcaatg tgactggttg
     7021 aagaatgaca aaatctaagt tagttcgttt cagccattca tctgcataat gtttagtaat
     7081 ataataatct gtcatggact caggccattt tgctggttca tctgcataaa cagcactgac
     7141 catcagatag cgagagacat gcgcattttc agctgcgatc actgttttga ttgcgccatc
     7201 caagtctact tgcagaagat tctttccttg agagccggca gcaaaaatca atacatcgat
     7261 gcctttgaat gcttcggtca ttttttccac ggaccaagtt aaatcaaaag agacatacga
     7321 aacattctct ttttttacta atgtttggga agcaacatct ctcacccctg caaaaatttc
     7381 atgggaactt ggagctaaat cttttatcag gtgtcggcca atttgtccgt ttgcacctac
     7441 aacaaaaatt ttcatttgga tcatcctttc gacgtcaaat ctttccattc aatagtaaca
     7501 ccaaaagaga aaaaaatcag agatcggcta cgatgactat cagttagtaa gagaagggtg
     7561 cttgaagtat cttaacaaga cattgcatac tgctgcagtc gcaagacctg cgacgattcc
     7621 ttcaggtata ccacttgtaa aaataaccgc tagaatagct gaataaacag catttaaatt
     7681 tgttccgatg actttggcat aatcttgctg gaataaaaag tagatcatat tcatcactaa
     7741 aatcgtattg gtcaatgaac cggaaagtcc agctaagaat aaagaaacgg aatctgcttt
     7801 ttgtttcatt agtttgcgca gacctttata aacgaaataa ggaaccaccc caatcaagat
     7861 gcgaggaata aacgcgatca gcaaggcttt ccagcttcca tgttctgttc cgatgactgg
     7921 gataaacgga gaaaagacaa aagacaaagg tgtttggata actgtgctac ggatcatgct
     7981 gatcagtcca aatgtaccgc caagaaaagc acctagtctt ggtcctaaaa cgatcgaagc
     8041 aatgattaca ggaatatgca tggttgttgc attgattgga ccgataggga taaaacctaa
     8101 aggcgtaaca gctaataaaa tcaaaatagc taaaaacatc gcagttaatg taaagttttt
     8161 tgtatttttc ataagtactc cttttttcta agcatttctt tcattaagag tcagcatgac
     8221 ctgttcgatg atcgtctgaa tatcagctaa ggcacctttt ccaaaatctc cacaagctaa
     8281 aagagattct cttggttcaa tttcctgata gccgacagat ctcaatgttt ccagattacg
     8341 ctgagtgatc gggttttgat acatattcgt gttcatggca ggggcaatca attttggtgt
     8401 tcccactgga agcgccattg cgactgttga aagaagatcg tctgcaatgc cgtttgctag
     8461 cttgccgata atgtttgctg tagcaggcgc gatgagaaac aagtcagctt gttttgctag
     8521 ttcgatatga ttgattacct gcggatttga ttcttgcatg acgtctgtat ggactgcgcg
     8581 cttcgacaga gattgcaagg ttaatggcgt gataaaatgt gtgctgcttt tagacataat
     8641 cacatctacc tgggcaccta atttgacgaa ttgacttgta agatcagcgc ttttgtaagc
     8701 agaaatactt ccagtaacgc ctagtaagat ctttttcatt ttgattcctc cagtctaatt
     8761 ttctcagtaa tcaatgcagc aatctcagat ttactttgag cttcttccac tgttccatct
     8821 ttagataaga gatagccgtg gtgtgtctcg ccatggattt ccgtcaaatc atttgcgaaa
     8881 ataaaatcag ttttgttatt tatcagacta ttttttgcga ctgccagaag agattccttt
     8941 ggtacatcta ctaatagttt gaaaccaatg atttttgctt ttggctgcca ttcacgtaga
     9001 taagaaatga tttttggtgt tttcttcaaa acgagcacaa gatgctcggt gttagaagag
     9061 atctttgaaa cagtggtatc tgtctgttca ttttccgcaa accaattgtc cagttcttct
     9121 tgcgttgaag aagtgggtag gttttttgcc agctgttctt ctgaaaaact aaaagcagga
     9181 gtaaaatcac tgactgccat actgtggata atcgcatcgt agtgttcttt tttcgttagt
     9241 tcttctaatt gtaaaaataa gtccagtgca gattcgatcg tataaatcgt tatattggag
     9301 tgtcttttcg gtttcagtgc ttgtttcgta gtcacgtaat cgattgtggt atccggattg
     9361 gcagctaaat aatcagctaa tgcttgccct aatcgtcctg tcgaatgatt tgtaatagca
     9421 cgtacttgat cgattttttc agtcgttcct ccagctgtga ttagaatctt catttatggt
     9481 cacctacatt tactagtgtt cttccttggt gtgttccagc aagtagttta gatgctgttt
     9541 caggcaattg atccagagta atctcttgga cgaccagttc atctgcaatc tgcaaatccg
     9601 ccatctgttg ccataaagaa agacgttgat ctattggtac attgacagaa tcaattccaa
     9661 tcaactggat gtttcgcaga ataaaaggca agacagtcgc gtttagtttg attcccccag
     9721 cattgccaca taaagctact gcaccgttat aagagatcaa tggaagaaga ctgcttagct
     9781 gctcgccacc tactgtgtcg atgacatagt cgatttgctg ttttcctaaa gcttttgttg
     9841 tctctggcag aaattcatca ggtcgtacga cttgcaaagc acctagactt tttagccatg
     9901 tgacagactc ttcttttctg gaaaaggcaa tgattgactg gaatcctagt ttatgtaaca
     9961 gggctatcgc tgtactgcct acaccgccag aagcaccagt aacgacaact tttttatcgg
    10021 aggtcatacc ttgacggatt aaagcgttca cacacaacat agcagtaaac cctgctgtac
    10081 caaggatcat cgcttgacgc agcgtcatat tttttggtag aggaactaac cagtctcctg
    10141 gtactttttg atattggcta taaccgcctg gatgggtgac gccaagccca aaaccagtag
    10201 caagtacaag atcttctttt gaaaaacgtg gattgttact ttggacgaca gttccggata
    10261 aatcaatccc tggagtcatc ggatatgttc gtatcacctg accagactcg ctcattgcca
    10321 atgcatcttt ataattgaca tcagaatagg caatttttac caagacttca ttttcagcca
    10381 actcagtaaa tggcagatca ctgattgctg tatggaaaac aggttccttt attacctgaa
    10441 aagatcgaaa agcgttcatt cgtgagacac tccttttaaa attttctttt atcataccat
    10501 aaagcctact cttttattct tgttcttccg taaatttaga
//
LOCUS       DAZULY010000090        10510 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000090 DAZULY010000000
VERSION     DAZULY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.091"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            10..1128
                     /locus_tag="ACG81A_001493"
     CDS             10..1128
                     /locus_tag="ACG81A_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5014863.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1256..2632
                     /locus_tag="ACG81A_001494"
     CDS             1256..2632
                     /locus_tag="ACG81A_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS5014864.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3033..4613
                     /locus_tag="ACG81A_001495"
     CDS             3033..4613
                     /locus_tag="ACG81A_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFS5014865.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            4811..5034
                     /locus_tag="ACG81A_001496"
                     /pseudo
     CDS             4811..5034
                     /locus_tag="ACG81A_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5239..5559)
                     /locus_tag="ACG81A_001497"
     CDS             complement(5239..5559)
                     /locus_tag="ACG81A_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFS5014866.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(5707..7941)
                     /locus_tag="ACG81A_001498"
     CDS             complement(5707..7941)
                     /locus_tag="ACG81A_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5014867.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8420..8686
                     /locus_tag="ACG81A_001499"
     CDS             8420..8686
                     /locus_tag="ACG81A_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFS5014868.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            8686..10413
                     /gene="ptsP"
                     /locus_tag="ACG81A_001500"
     CDS             8686..10413
                     /gene="ptsP"
                     /locus_tag="ACG81A_001500"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS5014869.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3343 a         1836 c         2149 g         3182 t
ORIGIN      
        1 ggaaatctat tgtttgaaaa acttagacag tctaaactaa tgttttggtc gcttgaatta
       61 ttgatcatag ctgcattgat catgatttca tcagaaataa actttatttt cgaaccgatc
      121 gggacatttt tttccacgtt atttgcacct gtactgattg caggctttct ttactattta
      181 ttgaatccta ttgtcaagct gttgatgaaa gcaaaaatca aaagaatatg ggctgtcgca
      241 attgtgctgc tccttttagt tgcagcaatt atttggattt tacttagtgt catcccaagt
      301 ctagtacagc aaatttcctc attggcatct aatatgcctg atttcatcaa acaggtagaa
      361 agctggttaa aagaggtagc cgaattgcct ttgttcaagg aagtagatat caataaatat
      421 tttgagcaat tagacatttc ttacgggaca atcattcagc aattccttag tggagtatcc
      481 aatagtttag gttcgatcgt tggaacgatc gcttcggcaa ccgtagtaat cattacagca
      541 ccattcatct tgttctatat gttgaaagac ggagaaaaac tagtgccaaa catccaacag
      601 tttttcccag aaaaaagaag aaaccaaatt gtggagttat tggggcaact gaataaaaca
      661 ttggctaatt acattagcgg acaagcaatc gaatgtttat tcgttggtac cttcacattt
      721 ttaggatata tggtaattgg tgtggattat gctttcttat ttggggtcat tgcgggattg
      781 acaaacctaa ttccatattt aggaccttat ctaggactag cacctgcatt tctagtaaca
      841 gtcttcaatg atccggtaaa agcattactt tgttgtgtgg ttgtgctaat cgtccaacag
      901 ttggatggga atatcattta tcctaatgta attgggaaat cactgaaaat ccatccatta
      961 acgatcatta tcgttcttct agtagctgga aatattgccg gattgcttgg tatcttctta
     1021 ggtgttcctt tttacgcaat ctgcaagacg atcatttcct atataatcaa aatcgtcaaa
     1081 gaagacaagc aaaatgaaaa caagaagaaa attgctacga cctcgtaggt agttttagta
     1141 cacttgtttt tattgcaaag aaaattgctt tgtgatacca ttttaacgtg gctatagtgc
     1201 cacgtttttt tctatcgtaa actaaaaatt aattttataa ggagagaatg cgattatgaa
     1261 tgctgaccct gagagtcagt cgctgttagc gcagatttta ttattgatcg ttttgacatt
     1321 gatcaatgct tttttagcag cttccgaaat tgctgttgtt tcgatcaata agaatcgaat
     1381 cgagcaaaaa gctgatgaag gagatgtgaa atctcaaaag cttttaaaaa tattgcagaa
     1441 tccaaataat ttcctatcga caatccaagt aggaatcact ttggtcaaca ttttgtctgg
     1501 tgcttcacta gctaatactt tatcggaacg gttagctccg gtattaggtg gcggcacagc
     1561 ggcaaaaaat attgcaaata tcattgtgct ggctattttg acctatgtat cgattgtttt
     1621 tggagaattg taccctaaac gtattgcttt aaataaatca gaagaagtcg caaagttcac
     1681 ttcagggctg atacgcttga ttggtgtagt agctaagccc ttcgtctggc tgctttctgc
     1741 ttccacaagt ttattggcgc gtatcacgcc gatgactttt gatgatgaag attcaaaaat
     1801 gaccagagat gaaatgcgtt atatgcttga aaacgaaggc gtattgaata atgaagaatt
     1861 agaaatgctc caaggggttt tttctcttga tacgaaagtt gcccgagaag tcatggtccc
     1921 acgaacggat gcatttatga ttgatatcaa cgacactgtg gaagaaaatg tgaatgaagt
     1981 tttatccgaa aactattcaa gaatccctgt ttataacgaa gacaaagata aagtagtcgg
     2041 aatccttcat acaaagaatt tactaaaagc tgcccataaa ttcggctttg ataatttaga
     2101 tatcaagaaa atcatgcagg agcctttatt cgttcctgaa acggtcttta ttgatgatct
     2161 gttatatgaa atgaaaaaga cgcagaatca gatggctatt ttattagatg aatatggcgg
     2221 cgtcgttggg ttagcaacac tagaagacct tttagaagaa atcgtcggag aaatcgatga
     2281 tgaatcagat gaagttgaga atctttatga aaaaatcgat gaacacgaat atatcatcca
     2341 aggacgtatg ctgattgatg agttcaatga agcttttgac agtgatctgc atatgagtga
     2401 tgtagatacg atggctggtt atttgattac agcattaggc atgattccgg atgaaggcga
     2461 aaagttatcg tttgatgtcg ataatattac gctggtttct gaagaaatgg aaggttctcg
     2521 aatccttaaa atccgtgtca tttttcatga tccggaagaa acagaagcgg aacctgaaga
     2581 agaacgaaga tacttcagaa aagaattcga agatgacgag ccaagaagat aattggaaac
     2641 ttggttatga aagacgtgtt gcttgaataa acaagcaaca cgtcttttct tttagtcagc
     2701 tgacaaaaaa atatttgact cttatggttt ggtggctttt tcattcgctg aatccatgct
     2761 atactgattc agttgtagta gttgacaaag gttgtgaaaa agctatcctg cattaagtaa
     2821 taagagcagc caagcaaaaa cagcttttcg tatttttggc ttgggtgggc ttattgctga
     2881 taggctgtct ctagaacgcc gtttactcgg aaaaagagac tgctacacgg cttttgtcac
     2941 agccctatat gaaattgtct ttctttaaga gatcggacaa tctgtgccta gagaaaatat
     3001 aaataattga atggaagaaa tggagttagg ttatgaataa tccaaaatta aagcaacaag
     3061 tggacaatcg cagaactttt gcgatcattt cccatccgga tgctgggaaa acaacgatta
     3121 cagaacaatt actattgttt ggtggggcta tccgtcaagc cgggaccgta aaaggcaaaa
     3181 aaacaggtaa ttttgccaaa tcagactgga tggaaatcga gaaacaacgt gggatttctg
     3241 ttactagttc tgttatgcag tttgattatc aagggaaacg agtaaatatc ttggatacac
     3301 caggacatga agatttttct gaagatactt atcgtacttt gatggccgtg gatagtgcgg
     3361 tcatggtaat cgatagtgca aaagggatcg aagcacagac aaagaaatta ttccaagttg
     3421 taaaaaaacg tggcatcccg atatttactt ttatcaacaa actagatcgt gatggtcgtg
     3481 aaccattgga attgttagaa gaattagaag agctattaga tatcgaatct tatccgatga
     3541 attggccaat cggtatgggt aaagggctag aaggtcttta tgatatctac cataatcgtg
     3601 tagagtttta tcgtccggaa aattatcagg atgaacgatt agtagaattg gatgaagatg
     3661 gactgcttcc cgaaaatcat cctctaactg aaaattcctt atatgaacaa gtcttagaag
     3721 aagtagaact aatcaaagag gctggagatg catttaatca agaaaaaatt gctcgtggcg
     3781 atcagacacc agtatttttt ggttctgcat tgactaactt cggtgtacag acttttttag
     3841 agactttcgt tgaattggca ccagctcctt atggacacaa aacacaagat gatcagatgg
     3901 taagtcctta tgaggaagaa ttttcaggtt tcgtattcaa gatccaagca aatatgaatc
     3961 cagcccatcg tgaccgaatc gcttttgtcc gaatctgttc tggaacattc gaacgaggaa
     4021 tggatgtctg gttggaacgt acaaataaaa aactgaaatt aagcaatgtc acacaattta
     4081 tggcagattc gagagaaaac gtcgaaaaag cagtagcagg cgatatcatc ggtgtatatg
     4141 atacaggcaa ttatcagatt ggggatacat tgttcgaagg aaaactaaaa gttgcttatg
     4201 aagaactccc ttcatttact ccagagctgt ttatgaaagt gactgcaaaa aatgtcatga
     4261 aacaaaaatc cttccacaaa ggaatctatc aattagttca agaaggagcg attcagcttt
     4321 ataaaacata tctaacggaa gaatatatca ttggagctgt tggtcagcta caattcgaag
     4381 tattccaata tcgtatgtta aatgagtata acgccgaagt aatcatgtct ccaatgggac
     4441 acaaaatcgc tcgctggatc gatccagaag atttggatga gaagatgagt tcaagtcgca
     4501 atatcttggc tcgtgaccgt ttcgaccaac cgttgttctt attcgagaac caatttgcag
     4561 aacgctggtt tgcggataaa tatcccaatg taaaattaaa aagtttgatg taaagaagtc
     4621 ttgacacaag ctgccctgca tcaagtaata agaatagcca aacaaaaaca gcctctcata
     4681 ttttgtttag agccgtgacg gattttccgt gacggctctt tttactatat taaaatcata
     4741 gaaatgtcta atcaaaaaaa tataaaaatt attttgcgta catatgatta aagaaaggag
     4801 agtgtgacac atggaaacag aatggcaaag acgtgaaaga aaaaaacaga aaaagaagag
     4861 atgttacgat tatgtcgatt attagatgta aaagactggg acaaaaacga gcaaattgca
     4921 caaaaagtac gatcaatcat ccataaagag gaaaaagtta aaaataaaga cacgcgtaga
     4981 actgaaaatg aaaagagaaa agtacaaaag aaaagagaaa aatcaaaaag ctagaaagaa
     5041 aaagaggctg agacagaagc gtttaactcc aagaaataag ctggaattca caaaaatttg
     5101 aaaagcaatt ttcgtgaatt tcagcttatt tccgaaggag ttgcttctgc tttcgccgtt
     5161 tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg ctctttaatt
     5221 attatgttca aaatttgttt acacggctaa tgtagcgcct aattcatgtt ttggaatcga
     5281 gatttcaaca aggcctgcca attcgattac tttgatttca tcctcgtggt aaacggcgtc
     5341 tttgatttta tctaagaaat aatttttagc atcttcgatt tctttcggct ggattttacg
     5401 tttgtcattg atcagcagaa tttcattatg ttcaggggct tcagtgtaga caatagtggt
     5461 atttccggct gtgtagacct tgactagatg ggcatcagtg ttttcaagct ggtttaatac
     5521 aagacgggaa tggctgtttg ttacatttac caatttcatg gcgctcacct cttttatgta
     5581 tttttaattg cttgtttctc ttattataat ttctttttta taaaatgaac agtattttga
     5641 cttttctgaa aaaagaatga gacggcattt ctcaaagaaa taccgtctca cgctataaaa
     5701 ctggatttat tcagccgttt cttcggctga ttctttagcc aaacgttctg gtttagaagt
     5761 aacgatgatt tttccatctt tatccagttt tgctttcaac tcatggatag aagggtgatc
     5821 aagataaaat tcagcgatac catcttcgat ttgttcttgg atcgtgcgtc gaagaggacg
     5881 agcacccata gatggatcat agcctaagtc aactaatttt tctttgacat tcgtaggaac
     5941 ttcgatgtga agttgctgtg ctgccagcat ttggttgaca tcgtctagca ttagtgtgac
     6001 gattgtcatc aagttttctt ttgataatgc actgaattcg atgatcccgt caaaacggtt
     6061 cagaaattct ggtgtaaagt agttgtttaa ttggttcaat acagaacgag taacaccttc
     6121 gcgtgccgca ccaaaaccaa cgtttgcttc tactttacca gttccggcat tacttgtcat
     6181 aatgatgatc gtatctttaa agctgaccgt tcttccttgt gcatctgtca atcgaccatc
     6241 atctaagatt tgcaagaaca tatgcaaaac atctggatga gctttttcta cttcatctaa
     6301 caagacaaga ctgtaaggat ttctccgtac tttttctgtt aattgaccag cttcttcgta
     6361 tccgacataa cctggaggag aaccaatcaa tttagagaca ctatgttttt ccatatattc
     6421 agacatatcg aaacgaatca ttgaatcttg tgaaccgaat agttcgtagg caagttgttt
     6481 cgcaagctct gttttaccta caccagttgg tccaacaaat aagaaggagc cgattggacg
     6541 attctgtttg tttaatccaa cacggtttcg gcgaatcgct ttcgctactc gatctacagc
     6601 gttatcttgt ccgatgacat gagctttcaa gtcatctgct aagtttttca gttgtgtctg
     6661 ttctttttct tttaattcac ccactggaat acctgtccgt tgttcaacga ttttttccat
     6721 atctttttcg gtaatcacag gtgtttcctc ttcagtgagc tgacgttctt tcattttttg
     6781 aagtttattg atctgatcgc gataataagc tgctttttcg aaatcttctt ctcgagaagc
     6841 taaaactttt tgttcttctg cttcttgcag ttttttctcg attgttttag gatcaacgat
     6901 ttggatcgtc aagtttttct tagaacctgt ttcatctagc aaatcgatgg ctttatccgg
     6961 caagaagcga tcttggacgt aacgattgga taaagttgcg gctgcttcga tggcctcatc
     7021 agtatatttt acatgatgat agtcttcata acgtttttgc aaacctttca aaatactgat
     7081 cgtttcttcg acagttggtt catctacccg tacaggttgc atacgacgtt ctaaagcagc
     7141 gtctttttcg atgatgcggt actcatttaa ggtagtagca ccaaccattt gcaattctcc
     7201 tcgagctaaa gctggtttta aaatattacc agcatccata tttccatcac cagcagaacc
     7261 agcaccgacg atttcgtgaa cttcatctat gaataggatc acattttctg cttgacggat
     7321 ctcttcaatc aatttttgca tacgttcttc aaattgtcct cgtataccag taccttggac
     7381 taaagatacg acatctaaac gaatgacttc tttgtccatt aatttttgcg gtacatcacc
     7441 atctacgatt ttttgtgcta aaccttcaac aactgctgtt ttaccaacac ctggttctcc
     7501 aatcaagaca ggattgttct tggttcggcg atttaaaatt tcaatgactc gtttgatttc
     7561 gtcatcgcgt ccgatcacag gatcgatatc accttgtcgt gcttgttctg tgatattgat
     7621 tccgtattct cccaataaac cttctgtatt gccttgaggt ggctgaccac cattgaagtt
     7681 gttgtttccg ccaaattgtg ttggtggtgt ttgctcataa tttccttgct gttgcatttg
     7741 gcgagacatg gaacggaaaa gatcgtccaa actgccaaat ccaaatggat cattttgggc
     7801 catattagtc agacctccat tttgttgatt tttgattttt tgataacagc tctgacagta
     7861 atcgagttgt gtccgctgac cattgacagt ggcatacaag tgaatcgttg cctcattctt
     7921 gccgcagttt tgacaaagca tgaatcattc acgtcctttc acaaaaatgg gtacatccat
     7981 attttacatc tcgtttttca tgaggtaaaa gaataacccc tagtttttta agagatttat
     8041 gaggtagaaa aggtgaaatc tctattgacc attcctgacc tttgactctt attatactaa
     8101 aaataaaaag ggaagcaagt aattcgtttc atatgaaaac aaagagggtc aaaggaaaga
     8161 atgataaacg tcaggagaag agaaagcgac aagaaaaagg aaataaacac gtttgttata
     8221 aaatgtgaag aaaaggatga aaaagaatcc ggaataattt gaatactttt atctgaaatt
     8281 gaatgaaaac tgttgtatct aacgaaaaaa aatgttaatc tttacagatg aaagggtggc
     8341 atttgttagg attctctgaa ttactaaaaa agaaaaccct cacataaaaa caacactcac
     8401 ttaaaggaga ccgattaata tggaaaagaa agaatttcac gtagtagcag aaactgggat
     8461 ccacgctcgt ccagctacac tattagtaca aacagcaagc aaatttaact ctgatgtaaa
     8521 cttagagtat aaaggtaaat cagtaaactt gaaatcaatc atgggcgtta tgtctttagg
     8581 tgtaggtcaa ggttctgatg ttactattac tgcagaaggt gctgacgaag ctgatgcaat
     8641 ggcagcaatc gttgaaacaa tgaagaaaga aggcttatct gaataatggt tgaaatgcta
     8701 aaagggattg ccgctagtga cggtgtagcc gttgcaaaag cttacctgct agttcaaccg
     8761 gatttaacat tcagtaaggc aacagtagaa gatactgcgg ctgaagaagc tcgtttggat
     8821 gctgcattag ctaaatctac tgaagaattg cagcaaattc gtgaaaaagc agcgcaaagc
     8881 ttaggtgaag cagaagcgca agtatttgat gcccatttga tggttctttc agatccagaa
     8941 atggtaggtc aaatcaagca aaatattaaa gacaacagcg taaatgctga atcagcgcta
     9001 aaagaagtaa cggatatgta tatcggtatg ttcgaagcaa tggaagataa tgcttatatg
     9061 caagagcgtg ctgcagatat tcgtgacgtt gctaaacgta ttttagcgca cttattaggc
     9121 gttactttac caaatccatc aatgatcaac gaagaagtgg tcgttgttgc acatgacttg
     9181 actccaagtg atacagcaca attagatcgc aattttgtaa aagcgtttgt aacagacatt
     9241 ggtggacgta cttcacattc tgctatcatg gcacgttctt tagaaattcc agcaatcgtt
     9301 gggacaaaag aaattactgc taaagtcaaa gaaggcgata ttttggctgt caacgggatc
     9361 gaaggcgatg taatcatcga tcctacagat gaacaaaaag ctgaatttga aaaagcaggt
     9421 gcagattacg cagcacaaaa agcagaatgg gaaaaattaa aaaatgcaga aactgtcaca
     9481 gctgacggca agcattttga attagctgca aacatcggaa caccaaaaga tctagttggt
     9541 gtccataata atggtggaga agcagttggt ctgtaccgta ctgaattcct gtatatggat
     9601 tctccagact tcccaacaga agatgaccaa tatgaagctt acaaagctgt attagaaggt
     9661 atggaaggaa aacctgtagt tgttcgtaca atggacatcg gtggagataa agaactacca
     9721 tatctgcaat tacctcatga aatgaatcca ttcttaggat atcgtgcatt gcgtatcagt
     9781 ttatctgaac aaggcgacga aatgttccgt acacaaatgc gtgcattgct tcgtgcatct
     9841 gttcatggta atttacgtat catgttccct atggtagcta cactcaaaga attccgcgca
     9901 gcaaaagcga tctttgaaga agaaaaacaa aaattgataa gtgaaggcaa ggaagtatct
     9961 gatacgatcc aagtcggcat catgatcgag attccagcag cagcagtctt agcagacaag
    10021 tttgcaaaag aagtagattt cttctctgta ggtacgaacg atttgattca atacacaatg
    10081 gcagcagacc gtatgaacga acgcgtttct tacttgtacc aaccatataa cccatcaatt
    10141 ttacgtttga tcaaaaatgt aattgatgca gcacacgctg aaggaaaatg ggcaggtatg
    10201 tgtggagaaa tggctggaga tcaaacagct gttccattgc tagtaggtat gggtctagat
    10261 gagttctcaa tgagtgcgac atctattctt aaaacgcgta gtttaatgaa acgcttggac
    10321 acagcgaaaa tggctgaact tgctgatcgt gcattgaaag aatgcgatat gatggaagaa
    10381 gtcgttgaac ttgttcatga atatgtaaaa taaacaacgt atcaaaagct ggtgtggctt
    10441 gtaaaagagc tacgccagtt tttttgttct tttttaagaa aacgtttttg tcagtaagaa
    10501 tagacaaaaa
//
LOCUS       DAZULY010000091        10479 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000091 DAZULY010000000
VERSION     DAZULY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.092"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(186..671)
                     /locus_tag="ACG81A_001501"
     CDS             complement(186..671)
                     /locus_tag="ACG81A_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFS5014870.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    761..1106
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1151..1792
                     /locus_tag="ACG81A_001502"
     CDS             1151..1792
                     /locus_tag="ACG81A_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5014871.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1811..2446
                     /locus_tag="ACG81A_001503"
     CDS             1811..2446
                     /locus_tag="ACG81A_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5014872.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2464..3279
                     /locus_tag="ACG81A_001504"
     CDS             2464..3279
                     /locus_tag="ACG81A_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5014873.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3598..5034
                     /locus_tag="ACG81A_001505"
     CDS             3598..5034
                     /locus_tag="ACG81A_001505"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5014874.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5223..7670
                     /locus_tag="ACG81A_001506"
     CDS             5223..7670
                     /locus_tag="ACG81A_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFS5014875.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7900..8967
                     /gene="rlmN"
                     /locus_tag="ACG81A_001507"
     CDS             7900..8967
                     /gene="rlmN"
                     /locus_tag="ACG81A_001507"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFS5014876.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            8957..9163
                     /locus_tag="ACG81A_001508"
     CDS             8957..9163
                     /locus_tag="ACG81A_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014877.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9160..10263)
                     /locus_tag="ACG81A_001509"
     CDS             complement(9160..10263)
                     /locus_tag="ACG81A_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS5014878.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3456 a         1795 c         2280 g         2948 t
ORIGIN      
        1 tatgcttctt ttttcgtaga atcaataaat atggatttgg ctaactatgg aagaatactc
       61 gtattagttt tcagcgttca tactagttgt tacattgttt gattggccaa aagaatttct
      121 gatatatttt gtgctaaata aagaaaaaga cgcttgaatt gggaaataag aaaactactc
      181 tatcattaca aattttcata aactggcaga tgttctaagt aaacaagtat cttttcaaag
      241 tcggaaaaat ccataatcaa cgtagtggta ttgatatttg gatgaaagcc tacggtatct
      301 tgacgatcaa tagcagcatc gatcacgact tggatttttt tctctttgtc gtgcatcaat
      361 gctaatggag tcactgtacc tgctggaacg cctaggaggt caggaagctg ttcttcagaa
      421 acaaaagata agcgcttttc ttttaattgt tcagctagtt gttttaaatc ggcgtgcttt
      481 tcatcaggca agatgacaag atagatttgt tttccttttc ttgatttcaa taatagattt
      541 tttacttgcg gaccaggtag ggaaaaaggg acatttttga ctgacgtaat cgcaggatga
      601 tcgacttttt gaaaagagat acccagtttt tttagtagtt cataagcttc ttgttcggtt
      661 gcacgactca tcgcgcatca ccctcctttt tttattagtg tatcataaaa aagagttgac
      721 aaatgaaaaa taatcatgta aattacacgt aaagttaaaa caaatgacat cgaaaagaag
      781 agtagccgtt aggtgttttt agagagtctc tggttggtgg aaagaggcag cagttaaccg
      841 tgaaacacat cttggagtcg attttctgaa gcgtatagta ggaaaatccg gggagcccgt
      901 tacagctgcg gtcgttgcta gatgaccaga taaggctggc ttttccgtga ggagtcggta
      961 aaaaaaggtg gaaccacgtg attaacgtcc tttgatcttc ggatcaaagg actttttgtt
     1021 ttcatctgaa cggcaacgac tgtgtgaggt tttttctgca aggaaaaaat aaacagaggt
     1081 ggaaccacgt ataatacgtc ctcttggcat ttgccaagag gattttttta ttttaaagga
     1141 ggaaaagaag atggagtatt tgattgaagt tatgccgcaa ttattagtag gagcactcac
     1201 aacattgaaa ttatttttat ttaccttgat tggctcatta ccattaggga ttgttttagc
     1261 attaggattg tctttgcatt ttaagccgct acgctacttg cttcaaacat atgtatgggt
     1321 tatgcgggga acaccgctgt tgttgcaact gatttttatt ttttatgggt tgccgacgat
     1381 tgggatcgtt tttgatcgtt tcgaagcagc actcattgcc tttatcttaa actatgcagc
     1441 ttattttgca gagatctttc gtggagggat acaatcgatt cctcaaggtc aatatgaagc
     1501 agcgcaagtt ttgcaattaa gtccctttca aacggtgacg aaaatcgtca tcccacaagt
     1561 gacaaagatt gttttacctt ctgtagggaa tgaagttatc aacttggtca aagattcttc
     1621 cttgatctat atattaggtt taggtgacgt aatgcgtgct gggaaaatcg cgatggaacg
     1681 cgacgcgact ttactgcctt tgctaggcgt ggcaatcatc tatcttacat tgactggtat
     1741 tctaacaatc ataatgaaac aagttgaagg aaaattaaat tattaccgct aactgatagg
     1801 aggaagaaat atgattgaat tgaagaaagt gaacaaagca tttggtgaaa aaaaaataat
     1861 caaagatttg aatctaatca ttccagatgg gaaaacatta gctatcgtag gtccttccgg
     1921 tggcggtaaa accacgcttt tacgtatctt ggctggctta gaaagttctg acagcggaga
     1981 attttattta gacggtgtac cgttcaaccc attcgagctt aaaatgaaag aacaagttgt
     2041 aggagtcgta ttccaagact tccagctatt tccacatctg aatatttttg aaaacattac
     2101 cttagcacca aaaatggtat tgaaacagac aaaagaagaa tacaccaaaa aagcagaaga
     2161 attagcggct tcattaggga ttagtgattt attgaatatc tatccttatc agctttctgg
     2221 cggacaaaaa cagcgggtag cgattgccag agcattggct atgaatccac gggtattggc
     2281 ttatgatgaa ccaacaagtg ctttagatcc tgaattacgc cagcaagtcg agcgtttgat
     2341 cctatcattg aaagaagatg gggtaaccca aattattgtc acccatgatt tagtatttgc
     2401 agaaaatgtc ggtgatcaat tactaaaagt ttccccaatt cattaagaaa ggagaagata
     2461 gagatgaaaa aaaccagttt tctattcgct ttgatcgcag gtcttcttct ttttgcaggc
     2521 tgttcgaaca aaaaaacttc agcagataat tgggcgacta tcaacgaaga gaaaaaaatc
     2581 gttataggtt tagacgatac atttgttcct atgggattta gagataaaga cgggaagctc
     2641 acaggatttg atattgattt agccaaagca gtctttgaac aatatgggat tacggtagac
     2701 tttcagccca ttgactggtc aatgaaggaa tttgaactga acaatggaac gatcgatttg
     2761 atatggaacg gctattccaa aacgccggca agagaaaaga aagtgcaatt tacaaaacct
     2821 tatatggaaa acgatcaagt gctgatcaca cctaaagaat caggaatcac aaactttgga
     2881 caaatgaata ataagagatt aggtgcgcaa aatgggtcat ctggttatga tgtatttacg
     2941 aaacaaccag aagtattaaa agagatagtc aaaaaccaag atgctgtttt gtatgatagt
     3001 tttaatgaag cattgatcga tctgaaaagc agacgggtcg atggtctgct gatggataaa
     3061 gtatacgcag actattattt gaaacaaaga aacgaactag aagacttcaa cataacaaaa
     3121 ggttcttatg agagtgagga ttttgcagtt ggtgcacgca agtcagatag tacattagtg
     3181 aaaaaaatca atgaagccct agatcaacta gagaaaacag gtgaattcca aaaaatatcc
     3241 gataaatggt ttggcgaaga tgttacgccg agaaattaac aaatagggga ctgtgacaaa
     3301 agacgtgtca cagtcccttt ttccgaataa acagtgttct cctgccaact ccttgtgact
     3361 cggagctgaa tggcaggctc acaacctctt ggtaaacggt gttcaaaagc cagtttttcg
     3421 gtattaagcc aaatttatgc aaaaatgtag aaagccattt tcacaaaatt tttcttacta
     3481 ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg ttgtggaagg
     3541 ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa gagatgaatg
     3601 gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc ttctgcagcg
     3661 tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg ggatcgattt
     3721 gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt agaccactat
     3781 catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc ttaccgcttc
     3841 tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca agctgggctc
     3901 gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc tatgttgact
     3961 ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg ggaatcacgt
     4021 cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt ccgcgggaaa
     4081 gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg ttatttgtta
     4141 gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa ccatattgct
     4201 aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc tggaaaaatc
     4261 ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga aaatgttctg
     4321 gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta tatgtgggga
     4381 aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc aacgattgaa
     4441 gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat caattactat
     4501 caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa aatgaatacg
     4561 acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa aaaagttgag
     4621 aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg attaaggatc
     4681 ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga aaatggctta
     4741 ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg aatcaattac
     4801 ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga agtcctaggc
     4861 tattgcacct ggtcttatac agatttgctt agctggctga acggttatca aaagcgatat
     4921 ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg atataagaaa
     4981 gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct ttagttcaca
     5041 tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata aaatcattta
     5101 gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag ttctttcaaa
     5161 caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat gagggataat
     5221 aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa acgaaaaagc
     5281 aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg attttatttg
     5341 ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac ggctttcgga
     5401 tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta ggattttgga
     5461 ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt ggcggcatac
     5521 tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa ctccatactg
     5581 gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata aaacaaagtc
     5641 aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat tttacttact
     5701 tcttgattgc acagcctgga agttatttag cagcagtgct attactggca ggaggagcat
     5761 tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca attggagaac
     5821 gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa gcggcaaagg
     5881 aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct gcaaaagaag
     5941 ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag caaagggaaa
     6001 atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta agttttgttc
     6061 caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca gtaccagata
     6121 caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag gatgacggta
     6181 cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa ttaccgacag
     6241 ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa aaaattgaaa
     6301 aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc aaggtcgtca
     6361 aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta ggggtaaaag
     6421 taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg aaagatgtcc
     6481 ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca aatggaaaga
     6541 tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca gataaattac
     6601 tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat ctttcgaaga
     6661 tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca attaatggga
     6721 ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg atgatcgatc
     6781 cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg ccggtcgtga
     6841 cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg gaagaacgat
     6901 atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa tttgttcaac
     6961 agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta gtgatcgtag
     7021 atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg attattcgtt
     7081 tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa cgtccaagtg
     7141 tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca tttgcggtat
     7201 caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag ctcttagggc
     7261 gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg caaggagcat
     7321 tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag caagaagctc
     7381 attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct ccagaacagc
     7441 cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa acggcaagta
     7501 tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt ttagtagatg
     7561 aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg aaagtattca
     7621 ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa aacttgtgtg
     7681 atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata ccaaaagaaa
     7741 aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt agtttttgtg
     7801 agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg aatcatgtat
     7861 agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca gtccatttat
     7921 ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa aaaattccga
     7981 gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac agaaatgacg
     8041 aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa tcctctaaaa
     8101 cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga actccctgat
     8161 aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt ttgtgtgacg
     8221 acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct caaaaagaac
     8281 cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca ttattttgat
     8341 gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg tgagccattt
     8401 gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa aggattggca
     8461 attggtgctc ggcacattac agtttccact agtggattag cacacaaaat caaggaattc
     8521 gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa taatgaggtg
     8581 cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat ggaagctgtg
     8641 gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat gttgaatcaa
     8701 gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga taagaaaaaa
     8761 ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca atattctcgc
     8821 agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg cgtaaactgt
     8881 gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt gcgaagcaag
     8941 caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa ttaccataaa
     9001 aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag attctatagc
     9061 gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa atgtcctcaa
     9121 tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt tcttatatcc
     9181 ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct gtttaaattt
     9241 ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg ctttatctaa
     9301 gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata ctggaagttg
     9361 gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat ccatcatcaa
     9421 taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca ttttttctag
     9481 aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat tgttaactaa
     9541 aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa ttgcatatga
     9601 gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat
     9661 gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat cgccacgagc
     9721 agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc ataagtattt
     9781 atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg
     9841 aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg cagtgacggg
     9901 cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt tttcaccaat
     9961 gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt ctgctttttg
    10021 gacactttca tgctggatca actgttcaat atttccataa gttgaattac ccccgtacca
    10081 gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt tttctgcctt
    10141 tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt tgacgattcc
    10201 atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa cactttgact
    10261 catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct
    10321 gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat gaataaggtg
    10381 gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac aaaaacattg
    10441 aaaacgacaa taatgaattt ttgttcctct tagtatttc
//
LOCUS       DAZULY010000092        10458 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000092 DAZULY010000000
VERSION     DAZULY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.093"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            253..522
                     /gene="rpsN"
                     /locus_tag="ACG81A_001510"
     CDS             253..522
                     /gene="rpsN"
                     /locus_tag="ACG81A_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS5014879.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            537..716
                     /gene="rpmF"
                     /locus_tag="ACG81A_001511"
     CDS             537..716
                     /gene="rpmF"
                     /locus_tag="ACG81A_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS5014880.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            748..1125
                     /locus_tag="ACG81A_001512"
     CDS             748..1125
                     /locus_tag="ACG81A_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014881.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1146..1295
                     /gene="rpmG"
                     /locus_tag="ACG81A_001513"
     CDS             1146..1295
                     /gene="rpmG"
                     /locus_tag="ACG81A_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS5014882.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1319..2302
                     /locus_tag="ACG81A_001514"
     CDS             1319..2302
                     /locus_tag="ACG81A_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS5014883.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2332..2454
                     /locus_tag="ACG81A_001515"
     CDS             2332..2454
                     /locus_tag="ACG81A_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS5014884.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2472..3995
                     /locus_tag="ACG81A_001516"
     CDS             2472..3995
                     /locus_tag="ACG81A_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFS5014885.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4019..4258
                     /locus_tag="ACG81A_001517"
     CDS             4019..4258
                     /locus_tag="ACG81A_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5014886.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4451..4912)
                     /locus_tag="ACG81A_001518"
     CDS             complement(4451..4912)
                     /locus_tag="ACG81A_001518"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5014887.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5107..5325)
                     /locus_tag="ACG81A_001519"
     CDS             complement(5107..5325)
                     /locus_tag="ACG81A_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5014888.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5443..7059
                     /locus_tag="ACG81A_001520"
     CDS             5443..7059
                     /locus_tag="ACG81A_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS5014889.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7300..8640)
                     /locus_tag="ACG81A_001521"
                     /pseudo
     CDS             complement(7300..8640)
                     /locus_tag="ACG81A_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9078..9224)
                     /locus_tag="ACG81A_001522"
     CDS             complement(9078..9224)
                     /locus_tag="ACG81A_001522"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014890.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9263..9457)
                     /locus_tag="ACG81A_001523"
     CDS             complement(9263..9457)
                     /locus_tag="ACG81A_001523"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5014891.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9367..9699)
                     /locus_tag="ACG81A_001524"
     CDS             complement(9367..9699)
                     /locus_tag="ACG81A_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5014892.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10213..10404)
                     /locus_tag="ACG81A_001525"
     CDS             complement(10213..10404)
                     /locus_tag="ACG81A_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5014893.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT         3468 a         1663 c         2046 g         3281 t
ORIGIN      
        1 gtatttgtgt ttgattggac taataaaagt gaattcttat ttttcagagc ctaaactgca
       61 tattctttta aatgatttga ttatctctat gtaccgataa attcgttttc aaatagttat
      121 ctattcatat gagttttatt tcatgttatt tattgttgac aagagcaact cttgttggta
      181 aagtatcagt gtcaaaacgt aacgattacg atttgaggtg tctaaatcaa aataaaagga
      241 gtataaaggt atatggcaaa aaagtcgaaa attgctaaag caaagaaaca aatggcgatg
      301 attgaaaagt atgcagataa acgtcaggaa ttaaaagccg caggagatcg caccgctctc
      361 gctaaattgc caagagattc aaatccgaat cgtctgaggc ttcgtgatca gacggatggt
      421 cgtccaagag ggtatatgcg aaagttcggt atgtcccgta ttaagtttag agaacttgcc
      481 catcaagggt tgattcctgg agtaaaaaaa gcaagttggt aaaaggagga aatatcatgg
      541 cagtaccagc aagaaaaaca tctaaggcga agaaacggtt aagaagaact catcaaactg
      601 tagtaaaacc agaaatttca tttgacgaag cgaatggtga ttatagacgt agccatcatg
      661 tatctttaaa agggtactat aatggaaaga aagtaattaa aggagcttca aaatagttta
      721 atatataact tttaggaggg aatcaaaatg gaaattattt acccaccttt agttgaagaa
      781 ggattaaagt attatcttga aacgacccag caatcgctag ataaatgcac tttttatcgt
      841 tcgatggtag agagaggaat tattactgaa acgggtttac cgacccaaca ggcaattgaa
      901 aatggattag tcaaagatta ctatgaggac caaggcttat catttgatga ttttttaaga
      961 atctatccaa tttttgaaga gtatgacgag gagttatttc aatgtatcga tggttattgg
     1021 gagatacctg ttgatatgaa agaaaattta gtttcacaat tggaatctgg cgagttaact
     1081 tttgaagatg cacaacaaat tcaagcatat cttgaagatc gataagtatt gaaggaggaa
     1141 gatttatgcg tcaaaatatt attttggaat gtgtcgaaac tggagaacgt ctgtatctca
     1201 ccagcaaaaa taaacggaat aaccctgaac ggttagaatt aaaaaagtat tcgccaaaat
     1261 tgcgcagacg ggcaattttt aaagaagtga aatagctaag tggtaaaaag gtgacaaaat
     1321 gggcatacca gttacaatcg tttctgggtt tttaggggct ggaaaaacaa cgctaatcaa
     1381 tcaagccttg caagtctcac cctaccccag ggaagaaatt attataattg aaaatgagtt
     1441 tggtgaagtt gggattgatc acaaattatt agttcatagc gaggaacggg tgttacaact
     1501 aaataatgga tgtatgtgtt gcagtcttcg cagtgattta ttagcaatat tgatttcttt
     1561 actggaaaca atagaagaac atcaacaacc aatttcacaa atcatcattg aaactacagg
     1621 tatagcagat ccccaaccaa tcattcaaac acttttaaca gcgcctcaac taagaggaag
     1681 agtatatatc gatagtttat tgactgttat tgatagtgac agttttgaac ttattgaaag
     1741 agaaccacaa gcccttaagc aacttgtcat ggcagatcgc atttttgtgt ctagaaaaca
     1801 aaacttgaat ttatctgcac taaaaaaatt acagagagaa atcaaaagta ttaacccttt
     1861 atccgatatt cgaatgttca cacatagtga aacgattcaa aaaaaggatt tttataattt
     1921 gaaaaaattt aatcatccct tagtttttga aagtgattca tcagaaaatc actataatgg
     1981 caaatactat catcaacacc atcaagtaca tgagtttaaa tcaattctct taacttcaga
     2041 tcaagacata aaaaaagatt ttttactcca atggattgac tggctgattt taaccaacca
     2101 agggagcctg tatcgtttta aaggattgat tgctttacaa gagcaggatt ttatagttgc
     2161 catgcaagag gtaaacgaac aggttaattt tcaatacacc actcgacaaa aagaccaaac
     2221 cactattatt ctaatcggaa aaatgttgga tgaagaaagg atcaaacaga gttttgataa
     2281 attactggcg gaaagcaaat aatgataagt agtgattggg ggtaatttct aatggagaaa
     2341 acggatatat ctagtgcata tcgacgactt aaaagcccga atattaaaac tagaaaaagg
     2401 gcattaaaaa ttatcaaaga acataaacga aataaaatga agaaattagc ataattaagg
     2461 aaggaaaaag aatgaagaaa atcagtgtag gacttattgg gatagcagct ctaggattat
     2521 tgggagcttg ctccagcact aatgatgcaa aagtatcgaa tgataaagat ggaaaattag
     2581 aaattgtaac aacattctat ccaatgtatg atttcaccaa aaacatcgtc ggcgatgaag
     2641 cgaacgtgga tttaatggtt ccagcgggat cagaacctca tgactatgag ccctcagcta
     2701 aggatatggc aaaagctcat gatgctgatg tttttgtata tcataacgaa aacatggaat
     2761 catgggtacc aaaagctaaa gaaagttgga agaaagctgg gcctaatgta gtagaaggaa
     2821 caaaagacat gattttattg ccaggtagtg aagaagaaga tcatgatcac ggagaagaag
     2881 atcaccatca tgaactagat cctcatacgt gggtttcacc caaaatggca attaaggaag
     2941 tttcaaatat caaggatcag ttagttaaat tgtatccgaa aaaagcaaaa gtatttgaga
     3001 caaatgctga aaagtatttg actaaactga aacggttgga tgctgactat accactagtc
     3061 ttaaagaagc gaaacaaaaa agttttgtga cgcaacacgc agcctttgga tatttagctt
     3121 tggattatgg attgaaacaa gttcctattg ctggactaag tcctgaagaa gagccatctt
     3181 ctggtcgatt agctgaatta aaagagtatg ttaaaaaaaa taagatcaat tatatttact
     3241 ttgaaaagaa tgctaatgat aaaattgcaa aaactttggc caacgaagct gggataaaat
     3301 tggaagtact gaatcctctt gaaagtctta caaaagaaca aatggataat ggtgaagatt
     3361 atgtttcagt aatggaagac aatctaaaag cgttagaaaa aacaactatg gttgctggtg
     3421 aagaggtagt tccagaaaaa gaagcgaagg atgaaaagac agttgctaat gggtacttta
     3481 aagatgccga tgtgaaggat cgcgaattga gtgattatac aggcgaatgg caatctgttt
     3541 atccgttgtt aaaagatgga attttggatg aagttttcga ttacaaagca aaaatcaata
     3601 aagacatgac agctgccgag tacaaagatt attatactac tggctataag acagatattg
     3661 atactatcaa cattaaagac aacaccattg attttgtagt caacggtgaa catcatcaat
     3721 acacatacaa atatgttgga tacaaaattt tgaattatga aaaaggaaac cgtggtgttc
     3781 gttttaattt tgaaactgat gatgcaggtg ctggtcgttt taaatatatt caatttagtg
     3841 atcatggaat tgcaccaagt aaggcagagc atttccatat tttctttggt ggagaaagtc
     3901 aagaaaaact ctataatgaa ttgcataatt ggcctacttt ctatccagct tcattgtcta
     3961 aacatgaaat tgctcaagaa atgatggctc attaaaattc aatttttgga ggaaatacat
     4021 ggtaagaaaa gtattattgt ctatggattc caagaatatt gatgcttatc ggcgttatcg
     4081 atttgcaaag caaatgagat taaatgggag tacattagct gatattgctg caactttaca
     4141 tgttagtatt gacacagttc atagagtatt aaaacgggat cctgattgtt gttttatcaa
     4201 ggtcaatact ggaagaagta gacgtttagc aaaacggcat aatagcttgg ccaattaatt
     4261 gataatacca gagtagatca tttttccctt atttggattt gatctgcttt ttattatgtc
     4321 ttgaattgtc aaatcaagcg caacacttta tcaaagaaga cttcataagg tgttctccaa
     4381 tctaaacact ttctaggtct gttattcagt ttttcaatca tctgatttat cgttgagtta
     4441 tctaccgtag ctatctcttt cgattttggc aagtattctc gaatgagtct attcgtattt
     4501 taatttgttc attgttgcca agggcatgag gatctggaaa ataaaagggc atattattca
     4561 atgcttctgt aactgcgaaa tgattggaga attcttttcc tctatctgga gtgatcgttt
     4621 tgaccgcctc atttggaata ctgcttaaaa gttcaatcat tttctcgctc acataagcgg
     4681 ctttctttct ggggaccttc tttcctgcta aaaggaagca tgattttctg tctgtcaaag
     4741 tcactaaaca agtaccacca gttttgccag ctacaatatc ggctttccaa tgaccaaaat
     4801 cacttctatc atctgcggat tttggacggc catggatcga atgagtaagg caaatctttc
     4861 cacgagactc gacatgattt tttgtatgtc ttgtttttcc tcgatggtgc aatgaacgga
     4921 tcaggccttt attgccgtgt gagaggggtt ctgtatcgaa aagatcattg caaattgcac
     4981 gttagatcgt gataaaacta attgaaaagt ccatatgttc atgcttcaaa cgattcgaga
     5041 tttcctctgg cgaccactga aaatcgacaa ataaatgacg aacaattgac caaactcacg
     5101 gatcatttag aatgtgataa cgtccgcatt tttttcttcg ataagcataa tttttttccg
     5161 ctttggaagg tgaatagttt ttttcttgtt tcttctcagt tcatgtaaaa tagtaaacgg
     5221 gctatgctga atgtcgttgc caattatact aatagaaaat ccttcttcgt gaagcagaaa
     5281 gattctttct cgttcgagta tagtaagatg acgatactgg ctcataatta ttttccttct
     5341 aggagtgttc tcgacaaact tatactagtt ggatatcgat tatgactcat ttttatgtgt
     5401 tgcacttaaa gtgtaaattc aaggtataga aagaggttcc ctatgaaaaa aatacctatt
     5461 acaatcttaa ctggctatct tggttcagga aaaacgatac ttctaaatca tattctgtac
     5521 ggtgatcacg gaaaggaaat tgctgtcatt gtaaatgaca taggggaagt aaatgttgat
     5581 ggggtattag tcgacaagaa aggttttaaa agaactggag aaaaacttgt ttcgatgccg
     5641 aatggatgca tttgttgtac attacgcgaa gatttgattg ttgaactaga tcaattagtg
     5701 caattttctg acttgaatca aattgttatt gaagcttcag gaatcagcaa accaattcca
     5761 attattcaag caattatatt agctaaaaac cctttagaca ttgatttgac ccaacgatta
     5821 gagattggtg ccattattat agtagtggat gtaaaacgtt tttgggataa tttcgaaacg
     5881 ggtgaaaaga ttcaggaaag aaaagctgaa ggtaatgtag ttgaagaaga acaggatatt
     5941 cgtggcttgt taattgatca attcgagtgt tgtaacatat tacttttaaa taatagtgac
     6001 ttagtgtcaa ccaagacgat ttacaaaatt aacggtctaa ttgctaagtt acagtccaat
     6061 gccaaaataa ttaggacaat taaaggtgaa gtagacttat cgaaaattct aaatacaaat
     6121 ctttttaata tagatgagac ttcctattca gtgggctggt tcaaagagtt agagcttggc
     6181 tatcaaaatc atattcctga aacagaagaa tatggtatct caagttttat ctatcgaaat
     6241 cggttactcc ttgacgcaca aaaatttgcc catcttacgg ataaaaattt tcctggcaat
     6301 attacaagag caaaagggtt tctttggttt gcccaacata gtgatttttc gacgatcttt
     6361 gaaacatcgg gtagctataa aagtattgaa tcattaaatt attgggtagc tagtttacct
     6421 aaaattgagt gtcaaatgat tatacaaaaa aatcccgaat tgaaggaagc ctggcatccg
     6481 ttgtttggtg aacgcattaa taaactagtt tttattggta tgaagatgga tcaacaatgt
     6541 attaaggaac aacttgaggg ttgtttaact gattctctta atgcagataa tttccgaaaa
     6601 atttcaccat ttccatggaa aaagtctaat atgtttagcc agatatcgtt aatgaaattt
     6661 ttaagaaaag ctggtgtcaa gataggcgat gtcataatta ttggcatttt gattattacc
     6721 tcttttattc ccttatttgt attctctata gaaaaggaga atgaaacaac tgaagatgct
     6781 atcaaatatg ccatcgttcg aattgatggc aaggaaatgg atcgcttcga tttggataag
     6841 atcaatcata aactcgtgaa ttatcatcct gcgaagggac aatataatat tgttgagatt
     6901 aaagatggaa gaattcgagt taaagaagat aatagtcctg atcaaatcgc ggttagaact
     6961 ggatggatct caaaacccgg tcaaacgagt atttgtttac cccataagtt agttattagt
     7021 attgagaaaa aagaaagcac agattactac atttactaat ctggaaatag atgaggaggt
     7081 ttttaataag aaaaagatat tattattaat aatagcaggg gctttattaa gtgtgttaag
     7141 cgcttgtgga caagaaaaag aagcggtgac aacaggcaca agtatcttta acgggacctg
     7201 caatggttta gttaacagtc ggttagatta cttattttgg ctcttcgtca atgagtgttg
     7261 gtgaagaaat tcaatagaat aatcgagata ctcacacggc tagacagcta tcttgtctag
     7321 ccgtgttgtt gttctggctt tagtcagttg ggtagtattc ttagttttct taaaatattt
     7381 tcctcgatat aaaaagattc gttctcggta gttttgaaag tttctatagc cataggcaat
     7441 tcgttttagt actttgatat ggttattcaa acattctata gggccgttgg aatagggaac
     7501 gcgcaacgct cgtttgattt ctgtttggta tttcttgaag gtcgtgaaca ctggttggta
     7561 gtgctctggc aactccttgt agtcttcttt taggagcgcg acgaattcag gaacatcttg
     7621 tcttctgata acggaaagaa aatcctggta gacttcgtag cctcgcttta aggaatcatc
     7681 ataagataat agccgatcga ctatctctgc ttctgtcaga tgatctcgaa atgacggacg
     7741 ccagtagcga tgctgacctt tcaattccca cgcattcttt tgaagtagtt tccagtattt
     7801 ctttaagtgc ttcgaccgac gatccccttt tttaaaggtc ttcattactt gaattcggtg
     7861 gttggtaaaa gcccgaccaa tgtgttggct aatatgaaag cgatcggcga cgattcgggc
     7921 attcggaaaa cactctttca ccaatgtgcg atagggtgta tagaaatctg aaaccactag
     7981 acgcacttgt tcacggactt ttatagggta gcgcaagaaa tagtcgcgta aaggattgag
     8041 ttgccgattt tccacaatat caatcagttg atgggtcttg ccgtccatca tgacaaagct
     8101 catggctccc gagacatttt tcactgattt gaattcatcg aaacacacca cttctggtaa
     8161 cagctggctc aaagaaatgg ttttaggccg aaggctgcga agtattcgaa tgacagtggt
     8221 cggagaaatg tgcttcatac gtgcgatcaa agacatagaa agaggttcgc ttaatagctc
     8281 aagaatggct tgtttgactc tgcgagcaat ggagcagcga cgatcactaa ctgaatcttc
     8341 tgctaaaaaa gtctttccac agtgtttaca tttgaagcgc tgtttgttaa tccgcagaat
     8401 ggtcttgtag ttactgacat cattcaataa gatggatact tttttccagc cctatttaat
     8461 gatcgtttgt tcattttgat agtggcgatg aaagcattcc tccggttgat aggtcaatgt
     8521 gccatcaaaa acaaaacatg tttcgtcatt gattcgctct ttgcgaaagg catcttcatg
     8581 aaagataaga tttaggtcta gaatatctag taactctttg gtaaactctg aataggacat
     8641 cttgaaacac tcctttgaat ggttttcgtc gactttattc taaaggattt taaggtgtct
     8701 gtttgcgttt attagaaaaa agaaaaagta ttggcaacag aaggcgtcac caacactaga
     8761 tattatagat cccttatttt cttcgcttca aattgtcaag gagtcatcat aaattttgta
     8821 tggattcttt tgtttgggta gtagtcttct aaataatctt ggattaacac cttcatctct
     8881 attttacttt tatatttgtt ggagtcgatt aattctcatt tgattgatcg atgaaacgct
     8941 tcaattactg cattgtcaac aggagtccca gtttggctca ctgaatgtgt gatgtggtgg
     9001 tcgttcaaca gctcttgata aataaaagac cgatgctggc tacctctatc ggaatgaatt
     9061 accgtaggca attctcctta ataaatttgt aatggacgga cgacaatctc ttttttcatg
     9121 tgtgaatcta ccatatagta acgtagaata cgagttgcta agtcaatata ggtggacaaa
     9181 tagaggcgac cgtcattaca tggaatgtaa gtaatatcca tcacccaaac agcatcaatt
     9241 tggttctgtt caaattatcg attcaacatt tttttaccat ttctttgatt gttttttctc
     9301 taccatgaga agatttgcgt cgatgaaatc ctcttgtgca taaattcatt tcacgcataa
     9361 gaacagctac aacacgttta ttgatttcga ttccttcatc gtaaagtttt tgagtgatcc
     9421 ctggagatcc gtatcgatct ttgtgttcgt agaacactcg tcttcctctt ttagtcgttt
     9481 attttcctgt tcaagaatgt agaaatctgc tttatcaggt aatctttttc cttgtccagc
     9541 aaaagcattt ttcccgtatc gacgatattc tgaaacccat cgggaaattg ttggacgaga
     9601 gatattgtat tcttcagcaa ttttcttggt agttactttt ttctcaagga tatcctgtga
     9661 aatctttagt ttaaatttac gatcatatgt ttttctcaat agagttcatt ccttccggta
     9721 acgtaactga tagtcaagaa aagtgtatca tagcccaact tagtatcagg gtttgtcaac
     9781 taaactgtgg aagttgatag ataagagttt ttaacaacca aaattctctt gactattttt
     9841 gaaatcagac aaattttgat tggacacata gttgaataaa tctaccgtcg ttacggaagg
     9901 taaatcgcat gcttttcaat tctagaggag aagtcctcgt tgaagtacca tagcccagtt
     9961 ctggattcga cctcctgccc acttcatacg taaataaagt aattttagga gtgagactgt
    10021 gacaaaagtt atttttaagc taaaaacgaa ttaaaaaacg ttgataatta aaatcattcg
    10081 ttttagattc tatgaatcat tatagaacgc tgtaaaatcg atactttata attataaaga
    10141 agatagtttc aaatttattg ttcgaaattt gaaactatct ttttacttat gtcacagact
    10201 cccttagttg agttattctt taatttcgaa tttcgagata gctagatata ttgcaagcat
    10261 acattcttga taacaatctt gatcatattt tccagttctc caggattgct tatgaatcaa
    10321 agagctaaat cttttgagaa ttgcctcaac agcttcttca tttccagcct ttgctaaaat
    10381 gaaatcttct tttaaagaat tcataaatct accttcttcc catggactta taatctattt
    10441 ctttgggaag gaggagaa
//
LOCUS       DAZULY010000093        10446 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000093 DAZULY010000000
VERSION     DAZULY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.094"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..137
                     /locus_tag="ACG81A_001526"
     CDS             <1..137
                     /locus_tag="ACG81A_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFS5014894.1"
                     /translation="SLATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKAMRKRVSR
                     "
     gene            complement(199..2010)
                     /locus_tag="ACG81A_001527"
     CDS             complement(199..2010)
                     /locus_tag="ACG81A_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFS5014895.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2007..2372)
                     /locus_tag="ACG81A_001528"
     CDS             complement(2007..2372)
                     /locus_tag="ACG81A_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFS5014896.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2535..2930)
                     /locus_tag="ACG81A_001529"
     CDS             complement(2535..2930)
                     /locus_tag="ACG81A_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS5014897.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2948..3910)
                     /locus_tag="ACG81A_001530"
     CDS             complement(2948..3910)
                     /locus_tag="ACG81A_001530"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFS5014898.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3910..4122)
                     /locus_tag="ACG81A_001531"
     CDS             complement(3910..4122)
                     /locus_tag="ACG81A_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFS5014899.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4143..4841)
                     /locus_tag="ACG81A_001532"
     CDS             complement(4143..4841)
                     /locus_tag="ACG81A_001532"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFS5014900.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4861..5403)
                     /locus_tag="ACG81A_001533"
     CDS             complement(4861..5403)
                     /locus_tag="ACG81A_001533"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFS5014901.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5535..6539)
                     /locus_tag="ACG81A_001534"
     CDS             complement(5535..6539)
                     /locus_tag="ACG81A_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS5014902.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6536..7525)
                     /locus_tag="ACG81A_001535"
     CDS             complement(6536..7525)
                     /locus_tag="ACG81A_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS5014903.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7522..8328)
                     /locus_tag="ACG81A_001536"
     CDS             complement(7522..8328)
                     /locus_tag="ACG81A_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5014904.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8494..9450
                     /locus_tag="ACG81A_001537"
     CDS             8494..9450
                     /locus_tag="ACG81A_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5014905.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9527..10045)
                     /locus_tag="ACG81A_001538"
     CDS             complement(9527..10045)
                     /locus_tag="ACG81A_001538"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFS5014906.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10133..10282)
                     /gene="rpmG"
                     /locus_tag="ACG81A_001539"
     CDS             complement(10133..10282)
                     /gene="rpmG"
                     /locus_tag="ACG81A_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS5014907.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
BASE COUNT         3241 a         2291 c         1782 g         3132 t
ORIGIN      
        1 gaagtctggc aacagccgct gccgttgctg gtttggttgc ttctgtgaaa aaaacagtga
       61 tcgatccaat tgacgaaaaa gaagcaatga tcgaagaaaa ccgtaaaaaa gcaatgcgta
      121 aacgcgtttc tcgttaataa ttatttaaaa atacgcatga agactttcta aaaaacagat
      181 aagtcatcat gcgtattttt atttgtcagt caatataagt tgtgctattt tatctgcctg
      241 tctcagattc catggatctg tataaggtct agctactgct gcagttagcc aatccacacc
      301 ttcaacaggt attccatagc aataaaagtc agactggatt tcccctgaag catcaatcac
      361 gcgattcgta cctcgctcaa catctatagc ttctgaatga taacttcctg tattatctga
      421 aaatgaaaaa gggcgaacaa gtttgttttc cttcaaattt tttatgacag gattacttga
      481 acgagaaaag cttgtttgtg gcaatcgagc ttcaatcaaa tagcgactgc tgatttcttt
      541 atcttgtaga gaaataacat agcggttgtg cttttgctga atatcaaatg gcggttcgat
      601 caaatgaaaa atacccgcat ctatcagtgc tgctaattct ttcgtccgaa tgattggcgg
      661 accgatcgtt aaaaagctat ttagatgagt aaaccatccc catagtattt ctttgtattc
      721 cttagcagaa aaaacgtgcc attcaatagc ctgccggatt ggatcacgcc agtcttttaa
      781 tgcatcaaat gttgaagcaa atgctcctgt gcaattgcct ttttccgctt cttttatctg
      841 cccttcaata aatattcggc tagcttctga aaagggctca ttctgatagg caagaggagt
      901 ttctaaatac gaccaatccc atctataagg gattagtgct ggatgctttc cgagacactc
      961 ctctgaagag agggagagaa aatcatgctc aaaatttttc tttggaatcg gcaagtgata
     1021 ttcttcgatg attttcttgt aataaaacaa ctcaacgtct tttttcagat agtcaaaaaa
     1081 tgtttcgcca gtcaaaaatc ccgactgatg ccattgctcc aggttttctt ttgttagtaa
     1141 ccgtggccat gccattgctc cgccttgctt ttgatttctt cctcgagggt aataagggag
     1201 cccccttctt gatccactgt atacaatcgg ttccttgcct gatggatgat agaccagttt
     1261 tcccttttcg ttctcgaagc gccctcctct tgatactgta aacaatccga cataatcaaa
     1321 aaaacttaag cctagacctc ttaaaaagac attttcttta ggcggtatct tcgaaagatc
     1381 tacatctgca ggattagaag gaagctgata cagtaaaaca tttttctttg cgtagtctac
     1441 ccacactctt tcttcttctg tctcatcatt ttcccagtga ccgcttgcta aaacaagctg
     1501 atcgatagaa tagctttgct gatctgtctg taaaagataa ccatcttttc ttttgcttac
     1561 tttttttact aaagaataaa caaactgaat agaagctgga aattcttctt gcaactttga
     1621 gaaaaaccag cgttgataga gtccgtataa acatcgagta gattgctgat ctttttccag
     1681 acgattgatt tcttctataa ttagttgctt atttttggtt accgtctgtt cgatataatc
     1741 tgtagcaggt cctttactcc attgaaacaa gttgggtccc tgtgttacca atcctccact
     1801 ggacaatgtt tcatctgtaa aaagcgtcac ttgttgagaa acagaattca ttaataactc
     1861 cggtggctga ttcaaccgcc aaactcgacc tccaggacca ttgggatcga aaagaaaaag
     1921 ttcaagagaa tgtctgtcct gattattatg gagaatcctt tctgctacac ttaatcctct
     1981 aggtccggca ccaatgatcc ctattttcat ttgcatgcct ccatttcgat aaattttttc
     2041 aataaaacag catggttctg atgttcattt tttgcaccat aaacaaaagt aatgactgga
     2101 tgttctttca taagttgact gagtttaaaa atagcttgct tcttttcttc ttcgaaaatc
     2161 aattcttgct ggtatttctt tgcaaatgaa gcaaatcgtt ctggtacatg gtcaaaagtt
     2221 ttgcgtgctt cattactagg tgcaatttct ttcaaccaca gatctaactg cgctttttct
     2281 tttgaaattc ctcgtggcca gaatctatcc acgagtattc tatagccatc ctctttttca
     2341 gcatcaaggt aaacacgttt taatttgatc atagttgttt ccttctttct accttttcgc
     2401 ttagcttata tatgtagata atagcacaaa ataaaaaaaa tgaacggcac aactatccat
     2461 tgcgccgttc atgtgatctt ggtcaaaatc taatgattga ttcggttttt aaagctgtca
     2521 tacaatcatc tgttttatac ttttttactt ttgatttttc cagaccatcc gtcatatcca
     2581 cccttaagta aataaatctc ttgataacct gctttacgta atttatttgc tgtacggatg
     2641 ctcaaacttt tcttttgatc gtaaatatag acaggctggt cttttcgaat agatcctaat
     2701 gaatctttca aaacagtgta agggatgttt cttgcaccta aaatatgccc agcatcaaac
     2761 acatcttttt cgcgaacatc gattacttgt gcttttctca ttccttctcg aaattcttct
     2821 tctgtcaaag tcgttgctga acgtttggcc attactcgta gatagacttc ccaaaggatg
     2881 attgcaagca caatcaatag caaaatgata ttaattaccc ataaaaccat gtaatgcctc
     2941 cgttttttta agcaaatttc aaggctaatg aggcagcacc aatgacacca gcttcattac
     3001 caagctctgc taatttgatt tttgttgatt ctcttacttg cgggaaagta aattctttaa
     3061 aatatttctc tacacggcta cgtaaaaatt ctcctgctgc cgatactcct ccacctaaca
     3121 cgatacttga cggatttaat gtattcccca agtttccgca agctaaacct aagtagaagc
     3181 atacgcgatc aacaaccatc aatgcaaacg gatcgtcttc ttgagccatt tcaaagatat
     3241 ctttacttgt tacttcttct ccgttgtcta gcattgcttt cagttttgaa tcccctgcgt
     3301 attcttctgc caagaaacga cctaggcgta ccacacctgt agcgctagat acagtttcta
     3361 aacatccgcg tttaccgcaa gtacattcga acccttcagg atccacagtg atatgtccga
     3421 tttctcctcc agctccgctc acgccgtgaa tcaaatttcc gccagcaacg atacctccgc
     3481 caacacctgt tcctagagta acgaatacaa cgtctggatc gttttctcca gcacctttcc
     3541 atcgttcacc taaagcagca acattcgcgt cattatcgat agcgaatttg attcctgttc
     3601 ctgcctcgat cagttttttc acaaattgaa cttctgtcca gtttaagtta tacgcaccaa
     3661 taaccgttcc agcttcgctg tccacgcttc ctggggtacc catgccgatt ccgataaagt
     3721 cttcggcttt catattatat aaattcaaac gatgattgat tgattcaatg atttcaggaa
     3781 cgatatgctt accatcatcc aaaatattgg tatcgatgct ccatttttgc tgaatctctc
     3841 cttctggtgt taaaattgca aattttgctg ttgttccacc taaatcaatc ccaataatct
     3901 tcttgtccat tacaaatttc ctttctttcg gctttcttca attcggtgct ctctttttaa
     3961 tatgcgccac gctgtcatgt atgtatcatg atcaatcaaa cggctatcat acagattacg
     4021 caattcgata gtcattaatt caatgtcata gatgcgtgca cccacataga taaagatgcc
     4081 aaattttttg aacagctgct gtacatcata taagctctcc atctttaagc acttccttca
     4141 tattatacaa gtaaaccata tttttttaaa cccagtaaaa tacatattac aaaaagaaat
     4201 acaaaaacta gtgtagaaag aatcctttgg tgtatcgaat agcttgtctg ttgtttcggt
     4261 agacccaaaa cattccctaa cactaatccg ccaattagcc caccgacatg tccccaaaca
     4321 tctacagagg tatccagaag accaaaaaca aatgtcaaca caataaataa aagaaaatgt
     4381 ctgactagct gctggatcgc tgggttatgt ttaaaatgaa accctaagat aaacaacgca
     4441 ccgaacaaac cgaatatcga agtacttgca ccaccagaca gaacattcgc ttcgttaaaa
     4501 gcaaagcttg ctgtatttcc cattatcccg ctgaacatat aaatcaagaa aaaacgccaa
     4561 tggccataga ttgcctcgat ttgctgcccc ataaaataca gtatcagtga gttcattacg
     4621 aaatgcatca gtccaaaatg gatgaagatt ggtgtcacta aacgccacca ttcattgaag
     4681 tgaacaagat aaggaccata cattccggca taataaggga tctcaagtcg cggaaataat
     4741 tccatcatga ggaagaccgc tgtctgtatc cctaaaaaag cataggtcca taacggtcgt
     4801 cttaaccatg cacgcaattt tatttgctgt tgataattca tgaaaacctc ccatttcatt
     4861 ttagtcaata aacaattgtt ggatcttctg atcaaagctc tccggttgcc attgttcatt
     4921 caattgttca cgaaaaacaa ggctacatgt ttttttatga aaattattta aataccggtc
     4981 gtaataacca ccgccaaaac cgattcgata accggctcta ttgtagataa tacctggtac
     5041 aatgaggaga tcgatcatct ccggcaaata gagcttctct gattgtggct caagaacacc
     5101 aaaactcgaa cgtacaagaa cggactgttc atccatttca taaaacgcca tctgacgttt
     5161 cggcaaagtt ttaggtacaa ctattttctt ttgttctttt tctgctcgtt ggataaccgg
     5221 ctgtgtatcc agttcaaatt cattcgataa agtcgtccct atcacttttg ctgacttcca
     5281 gtactcgctg gcgaatagct gtttatagat tcgctgttct ttttcctttt tcttgccact
     5341 tttggccaga tttttcagct tttcgattcc tttccggcgt aatcttactt tcaattggtc
     5401 caaaaactca cccttccttc tctcataaaa tggtatagga aaaaaagaaa aatagcaatc
     5461 gaactttcaa ataaatgcat aaatagaata aaaagaagac ctagaaatga ttggccttcc
     5521 ttttttaatg aatttcaact gttcagcgcg tgtttttgtc tgataaaaag tgcatacaga
     5581 aaatatggcg caccaaaaag ggcaatcaaa attcctgtag gtacttcccc acttggtaga
     5641 atcactctag caaccatatc tgcactaagc agaaaaatgg aaccgaacaa accgctcaga
     5701 acaaaggtcc agttattttc tttacgtacg actagcttcg cagcatgagg agctagtagt
     5761 cctacaaaag ataaactacc tgtcaccgct acactaccgc ttgccaaagc aacagctaac
     5821 aacaaaagga ttttttgact tttagccacg ttaaggccta atccttgcgc gcgttcttga
     5881 ccaaatgata gcaatacaat cctctgtgca taaagtcctc ctagcggaat taagataacc
     5941 gcccatggaa gcaatgccca aacataaggc cagctcgcac cccagattga tcccgctaac
     6001 catgcattca caaatccata attttcctta gataattgga tcgtgcatag caatgttaat
     6061 gcagaaaggc cggcattaac ggcaataccg gacaataaga ttcgattcat acccaaaaag
     6121 ccatttcgat cataggccgc aagatagaca cagcaagcag ctaaaacacc acctagaaag
     6181 gctacaacta gcaatagaaa cggcagcgaa tagtgcgaaa agaaacctaa ataaagcatc
     6241 actaaaaatc ctgctccggc attaatgccc aatatactag aatctgccaa ttcatttctt
     6301 gttactcctt gcaagatata accagaaacg ccaagacaag ctcctgctaa taaactgatc
     6361 agtaatcgag gaactcgaaa ttctctaaca atcagcgcca aagaagatga ttctttccct
     6421 atgaacgtgg caaaaagctg atccaggtaa ataaaaggcg taccaatcat taaactacct
     6481 atagacagga tgacaagtac aaaaagcaag aaatagaacg tccaatatat ttttttcacc
     6541 gttgttccct ccttacttgt atcagtaaaa acggaacgcc aatcaatgca atgactaatc
     6601 caaaaggtgt ttcaaaaggg ggattgatca tcctcgctgt aaaatccgcc aacataacca
     6661 atccaccgcc aactaaaaaa gagacaggta gaacataacg atagtccgtt ccaaccattc
     6721 cgcgaacaac tggcggaacc aacagcccaa taaatgaaac aggtccgact aacgaaacgg
     6781 caatggctgc taacactgca actagacaga aaacaatcaa ccgcgttcgt ttaggatgac
     6841 ttccaagagc taatgcatgt gtttcactca aatgaatcaa agatattgct tttcctaatg
     6901 aaaaagaaac caaaacagca agtatatata aaggaaacaa tactgctaat tgtttccatg
     6961 tgatattggc agctccgcct acaaaccaaa aagcaagatc ctgctgcaga ttaaagaatt
     7021 gggtaagtgc ttggctgata gcagtaaaaa aagaactaat agctacgcca gctaaaatca
     7081 gctgtaaagg attcatgccc attttcgaaa actgcaaaaa accaaatacg agcaaagtta
     7141 cagcaaaaga acctgccaat gacattagaa acacagaaaa atcagaagct ttaggccaaa
     7201 aagcaaaact aagggccatt gccaacgaag cacccgcgtt gattcctagt aagcctgaat
     7261 ctgctaaagg atttcttgtc accccttgca tcaatgcacc gctcaatgca aaacaactcc
     7321 ctacaagaaa tgctcccgcc acacgaggca aacgaatatt tcgtatgatt tggtgacttt
     7381 gttttgatct atcaaaatca aacaacgcct gccaaaaatc tgctttttcc acagtcgctg
     7441 cgccatttga aagagcaaca aaaaaaataa tcaaaagaac aaaacagacc aaaagcagga
     7501 gtctattttt cttcatagtc ctcataattg atcctcctgc tcttttggca acaaatcata
     7561 ggttagaaaa actggtttat ttgttcttgg aagcgtgaca atcgttgcat caatctcaaa
     7621 tatttttttc atacactctt ttgtgatcac ttcttcaggt gtaccttctg tgatcaactt
     7681 accgtttttc attgctaata aataatctga aaaacgtgaa gcatgattaa tgtcatgaat
     7741 cgacatcaag atcgttgttt gtttcatttg attgatttca gtcaataact gtaggatctc
     7801 taattggtgt gccggatcta aaaatgttgt cggttcatcc aagatcaaga tatctgtttc
     7861 ctgagcaagc gccatagcga tccagacacg ctgggtttgt ccaccagaca gcgttgcgat
     7921 atcccgcata cgaaatccag ctagacctgt agcatccaac gcccactcaa ttgcttcatg
     7981 atcttgcgtg tccatgccac tgaacagttt ttgataaggg tatcgtccgt aagatactac
     8041 ttcttctact gtcatacctg tagggtgttc actagtttgt gctaataatg caattttttt
     8101 tgcaagttct tttgaactgt atgtagagat atcagagtta ttgacaatga tctttcctga
     8161 tttcacaggt agtatacggg taaaactttt caataaagta gattttccac ttccatttgg
     8221 cccaatcaaa ctagtgattt tcccttcagg aatcgtaaca gacatgtttt caatgatttt
     8281 tttgttttcg tagccggctg caatgtcagc tgctcttaat ctcgtcattt cacccatcct
     8341 ttcgctgaaa atcattctca attatgacgc tgtttcaaca tttcgtcaag cgctactttc
     8401 atatctatag ccaaatgaag cggttgacag cgttcgaaat atcaggaata ataaccaatg
     8461 agaaagattc tcaattatgt taggaggaaa gaaatgaaat taaaaacaaa agtattcaca
     8521 ttatctagca tattgctgct ggctggagta ctgagcgctt gctccccctc ttcagataaa
     8581 gaaacagcta caactgccaa ttcgacgact tcatccaata gtactcatac agtcacagat
     8641 acacttggac ataaagtaga gattccaaac gcgcccaaac ggatcatcgg cagttactta
     8701 gaagactatc tgattgcact aggggaaaaa cctatcgccc agtggactgt tggcagtggt
     8761 tccattcaag attatctgca agatgattta aaggatgtcc caacgatttc ttatgacctt
     8821 ccttatgaga aagtgttaag ctttgagcct gatttgctgt tgatttcttc ttccgctact
     8881 gtcgaaggcg ggaaatacga acaatatagc aaaatcgcgc caacttatgt tgtaaaaaac
     8941 ggaacagatg tcacatggga agaacaacta aaagatgtcg gcaaagcttt ggcaaaagaa
     9001 aaagaggcag aaaaaatcat ttccgattat caaaaatcca tcaagaaaac tcgagaagaa
     9061 ctagcagata aaatcgagaa caagacagct gcagtgttgt gggtgaccaa caactcagcc
     9121 tttatggtca gcaaaaatcg gtcaagtgga agaatcgtat acgacgatct ggaatttggt
     9181 gttcctgatc tagtggaaga ggtctccaaa aaagccactt ctgactggtc tgctgtttcc
     9241 tctgaaaagc tagctgaatt agatgctgac tacatcatcc ttgtgaacag tgacaaagat
     9301 gctgccatgt tcaatgaacc aagctggaag aatctaaaag cagtgacaga caatcatgta
     9361 ctgcaattcg gacctgaatc tagttggcta tataacggac ctattgccag tcaggaaatg
     9421 gtcaatgata tcaaagaaaa actaaattga ttttaactaa aaaaagcatc ttcacgatta
     9481 gtcgtgagat gctttttttc ttattaacaa actagacgca tagattttat tcttcttttg
     9541 aagcaaccct attttttact ctttcccatt tttgaaaacg atgggcatat agattttctt
     9601 gatccgttat cccctcatac tcctctataa gggcgaagtc tgaaaaatca atattcccaa
     9661 tatacgtatc gccttcaaac gtgctatcga tcaatgttcg ccagatccga ccagtgtaag
     9721 gaagaagttc ttgaaaaatc cgactaccac ccgaaacata gatatcttct gagatcgttt
     9781 gactatacga caaaagttca tcgatcgaat gcattacttc cgcattttct ttcgtgcgaa
     9841 aatttggttg cgttgtcaac acgatgatat ggcgataagg aagtgaaaga tcccccattc
     9901 cttcataggt ctttcttccc atcactaaaa ttcgatccat tgtatgttcg cgaaagaaac
     9961 gcatatcatt tggcaatcgc catggtaaca aaccattttt gccgatcaga ccatttttat
    10021 cttgtgccca catggaaata aacatattat ccgctcctta tgattttaga tgttttgaga
    10081 taaaaaaagt ctcaattctt tgaccagcaa ggaattgaga actttaaaaa cattattttg
    10141 tttcacggtg caaagtaact ttgcgttcgc gtgggcaata tttttgtttt tccaaacgat
    10201 caggattgtt acgtttgttt ttgcttgtta gatagttacg ttctttacaa gaagtacact
    10261 ctaaagtgat gtttacgcgc atgtttttcc ctcccatttt tacaagaatc ctctaagatg
    10321 actctcagac ttgattatct taccatgatt ttttctagat tgctagtgtt tttcacgatt
    10381 ttgttaagta aaactacttg acgataaata caacggtttt acttctcaaa caaaatcttt
    10441 ttcttt
//
LOCUS       DAZULY010000094        10391 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000094 DAZULY010000000
VERSION     DAZULY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.095"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(106..1053)
                     /locus_tag="ACG81A_001540"
     CDS             complement(106..1053)
                     /locus_tag="ACG81A_001540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5014908.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="ACG81A_001541"
     CDS             1299..1763
                     /locus_tag="ACG81A_001541"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFS5014909.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="ACG81A_001542"
     CDS             complement(1825..2868)
                     /locus_tag="ACG81A_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFS5014910.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(3245..4210)
                     /locus_tag="ACG81A_001543"
     CDS             complement(3245..4210)
                     /locus_tag="ACG81A_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFS5014911.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            4444..5289
                     /locus_tag="ACG81A_001544"
     CDS             4444..5289
                     /locus_tag="ACG81A_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS5014912.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            5480..6667
                     /locus_tag="ACG81A_001545"
     CDS             5480..6667
                     /locus_tag="ACG81A_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFS5014913.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            6673..7029
                     /locus_tag="ACG81A_001546"
     CDS             6673..7029
                     /locus_tag="ACG81A_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFS5014914.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            7031..7369
                     /locus_tag="ACG81A_001547"
     CDS             7031..7369
                     /locus_tag="ACG81A_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFS5014915.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    7445..7701
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7764..8798
                     /locus_tag="ACG81A_001548"
     CDS             7764..8798
                     /locus_tag="ACG81A_001548"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS5014916.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            8791..9477
                     /locus_tag="ACG81A_001549"
     CDS             8791..9477
                     /locus_tag="ACG81A_001549"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS5014917.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            9501..10349
                     /locus_tag="ACG81A_001550"
     CDS             9501..10349
                     /locus_tag="ACG81A_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS5014918.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
BASE COUNT         3438 a         1916 c         2185 g         2852 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa cattttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa taaacggtgt
     3001 tccagaagta gcttctacgg aaataagccg aaatttcaca aaaatttgag aagcaatttt
     3061 cgtaaattcc ttcttatttc ttgaagctga ccacttcttt tacgacctct tggtaaacgg
     3121 tgttcaaaag cttgcatctg cggtaataag cccaaccagt acaaaaacat gaacagctgt
     3181 ttttgtttgg ccgttcttat tacttgatgc agaacagctt tttcacaacc tattgaatcg
     3241 attattaagc gattttttta gaaacaaatt ttccatgttt gctatgttct ttaattaaga
     3301 aacgtacgta atgagcaaga acgtagaaga caactacact tgcaaacaga cattccgcaa
     3361 cttttaagaa atccaatacg ctactataga tacctaaact gttgaagtaa tgaacagtag
     3421 taaatatagc tgttgcagag ataacaagcg ggaatgtaaa ggctgcatag cttggataaa
     3481 aacgaagctt gaacaaacgt ggaagcatag acagcacaac gaaatacaat acttgagata
     3541 aagcgaacaa tgctacgaca agataaaaat taggttcagc aaagttttta agataacctg
     3601 ttaggcaaag agaaccaggt gctgcaacga tcgtaatcag cggacgtgtc ggatgagcca
     3661 tatttcttac gaaaaagaca cgtttgatga tgatcggcaa taagaacgca tagcagatca
     3721 atgaaatcca aaagaaaatt tttccaataa gtgggaagaa gttcccggca gttacagtga
     3781 tgaccccaaa tccaacgtaa atgataaacc agcttggata aattgcttca attgaaagat
     3841 cagctttgat aacatggtga taattgaaat aaaagacaag gatcacttgg aagataacag
     3901 cgcacagcca aatgaatttg acaattggtg caatactttc atatgaaaca aaataagtac
     3961 aaataaccat gagtgacatc gtaaaagttg gtgaaacgga ggcgatgatt ggatcatgca
     4021 acgtttgttt cgtgtgttcg aataagataa acaatttaac aaaaatgaat atcataagaa
     4081 tcatagagat accgccagtt agattgccta aagcaacatg gtgatagtct ttaaacagat
     4141 ttccaaggga agctaaccca aggatcaacc cgcagatcgg gatcggaata attttcaaaa
     4201 agttcttcat atataatatt ctccttttta tttaatagat tttagtacca agatgaacga
     4261 atacattgta aaatgaatag gaatgtatga ttacgagcaa gcagacagct gattgtcttc
     4321 atctaagtta ctagttggat cagcaactgt tttgttgtac atttctatta tagatggttt
     4381 tttgtatatt taaatcaaat agtttttata gtaattatca taaaaagtta tggaagtggt
     4441 gccatgttta agttattaac gacttttcga gcagtatatg aaacaagaaa tttttccaga
     4501 gcagcagaag tactgttttt atctcaacca gcagtctcga atcaaatcaa acagttggag
     4561 cgggaattga atatcgatct ttttcaacgt aacggaagaa aagagatgct tccaactaag
     4621 caagccgact tattatatga acggacactg gttttgttag aagaatggga agaaactcag
     4681 caaaaattgc tcaatgaaca aaatgaagaa gaagtatgtc ggatcgttgc ttcccacacc
     4741 tttgctgtct atcttttgcc tcagttgatg aaacatctga tcaaaaggtt tcctaaagtg
     4801 cattttgaga tcgaaatcac gaattctcat gaatcattag aacaagtagc gaaacatgaa
     4861 tccgattttg gttttatcga aaagccatta gagacttccg gcgtacaaag atactctttg
     4921 atggaagacc agctagtcat cgctggggac ccaaaaagca aattgtggct tgtaagagaa
     4981 gcaaactctg gtgtttttca ttacacgcag cgttattttg aagagatgaa tatccaagat
     5041 aagaagatga tcgtgaaaag taacgatgta attgttgcgt tgctgaagga aggaatcggt
     5101 cgctcgattt tgtctaagcg ctctgttcca gaaaatgtgc caatgactcc gttgaatttc
     5161 catcgatatt tttatttcat ccaacgaacc aatctggttt ctgatcagct gctagagatt
     5221 gccgaagaga tccttcgata caatgaaagc acagaaactt ctcacagcga ggagaatctc
     5281 agctggtaga taattgcatg tatcaaaaaa agttctatga tacactaaag ataagcagta
     5341 ggcttttgta ctgcatttgc tcaagcaact gcacaaacgg aaagttaatt tttcccaaat
     5401 gtctatggaa agactttcca ttatctaact ggaccgctat aataatcagc agtatggttt
     5461 ttaagaaaag ggatgaaaca tggaaaaaaa attaaaagct tcgaacgata atcgtattga
     5521 ttatggcgtg attttaccag tctttctgtt atgtttgatt ggtctgctct cattatatgt
     5581 agcacttagc catgatccga acaatccagc agtcttgaaa ggcgtaggga tgcaagttgt
     5641 ctggtattta gttggagcgg cagcaatcgt tgtcatcatg catttgaatt ccaaatggat
     5701 ctggaaactg actccttacc tttatggttt ggggttagtg gtaatgggac tcttgctcaa
     5761 attctatgat cagaatttag ctgcttcaac agggtctaaa aactggtttc gattcggtgc
     5821 gtttactttc cagccagcag agttgatgaa gattgcgtac atcttgatga tggcactgat
     5881 cgtaactaaa cataatacgc aagtaaaaga acggacgatg aaatcggact tttggctgat
     5941 tgggaagctc ttgatcgtga caataccggt tttagcattg atcatggccc aagatgactt
     6001 tggaacgatg cttgtctttc ttgcgatctt tggcgggatt ttcctgatgt ctggaatctc
     6061 ttggcgcatc atcgcacctg tagtaatctt ggcagtagtc gttggagcag gaacgatctt
     6121 cttggttacg actgaaggcg gaagagactt gctttataaa gttggattca aatcttatca
     6181 gtttgcacgt attgattcat ggctggaccc tttccatgat acgtcaggaa tgagttatca
     6241 gccggcccaa ggacttttgg caattggtac cggtggcttg tttggtaaag gattcaacgt
     6301 aagcaatatc tatgtgcctg ttcgtgagtc cgatatgatt tttaccgtta tcggtgaaaa
     6361 cttcggcttt atcggtggtg cattcgtcat tttcttgtat tttattttaa tctatcgtat
     6421 gattcgggtg tgtttcgata cgaataatga attttacgcc tatatcgcaa gcggtttgat
     6481 catgatgctg ctgttccatg tctttgagaa tattggagca aatatcggat tacttccttt
     6541 aacagggatc ccattgcctt tcattagtca gggaggatcc tcgattttag ggaacatgat
     6601 cggtatcggg ttgatattat cgatgagata ccaaaatgaa gcacctgcaa gatcttcaag
     6661 gaggtaagaa aattgtacac cttttactgg tacccgaaat gttcaacatg caaaaaagcc
     6721 aaagcgtggt tagaccagca tggcgttagt tatcaaaccg ttgatatgat caaagaagta
     6781 ccgaaggcct caacgattga gacttggcta gaaacgagtc aagtaccaat ccgtcgcctg
     6841 ttcaacacaa gcgggatgaa atatcgggaa cttggactga aagatcaagt aggcgagttt
     6901 acggcttcaa aagcgagcca agtcttatcg acagatggta tgctgatcaa gcgaccactg
     6961 atcttacgag aggaccagct tgttgcaatt ggattcaatg aaaaaattta tgaaggagaa
     7021 ctgatataaa atggcaggca agcatttgaa gaaaaaggac aacctttgga tcttatttaa
     7081 tggaaaagaa tactgtgttg gtttgacaaa tgaagcgcaa gaagaacttg gagacatcac
     7141 gtttgccaat ctgccaaaag caggacaatc gctaaaaaaa gatgaacctc tgatcgaagt
     7201 tgaagcggaa aaagcggtca atgaatttgc cagcccgcta acgggtacag tttcttctgt
     7261 taatgataaa atcggtgaag atatcaatat cttgaatgat tcagatgaaa tgaatgcgtg
     7321 gatcttaagt ttcaaagatg ttgatccaaa agaatttgac gaattataaa atcagaaaat
     7381 cacttgaaaa aagttgacac tgattcttcc agttgctata atcataagca atcctatatg
     7441 aaacgagaaa gctttgaaaa gaagagtaca tgtcacggac gtttgaagag agccctgttt
     7501 gctgaaaatg ggtaacgaaa atgagatgga agatggtctt agagcagagt aagcgagcgt
     7561 aagcaaactt actacgggag tgcccgttac agcgcgacag tatacggaaa gaagctaagt
     7621 ccaagtactg gaagaggctg ttattgcgaa gtaacggtaa atcaaggtgg taacacggaa
     7681 gttcaagctt tcgtcctttt gcttaaggca agagggcgaa ggcttttttg tttgaaaaat
     7741 cataagagaa agatgtgaat ggcatgtcat taatccaact aacgaatgtc aaaaagacat
     7801 tcaatgggaa aaacggaaac atccaagccg tcaatgacgt ttcactaaac gttgaaaaag
     7861 gggatattta tggaatcgtc ggctattctg gcgcaggaaa aagtacatta gtccgtctcc
     7921 tgaatggact tgagcttccc acgagcggcg aagtgatcgt aaacgatcaa gatattacaa
     7981 aattaaaaaa caaagaatta cgtacattcc gaaagaaaat cggtatgatc ttccagcatt
     8041 tcaacctgtt atggtcaaga actgtacttg aaaatatcca gctgccgctg gaattagctg
     8101 gcgtaccaaa gagcaaacgt aaagaacggg cagaagaact gttgcgttta gtcgggttag
     8161 aaggacgcgg acaagcatat ccttcacaac tttccggcgg acagaaacaa cgcgtcggta
     8221 tcgcacgagc attggcaaat gatcctgaga tcctgctttg tgatgaggct actagcgcat
     8281 tggacccgca aacaacagaa gaagtattag acttattgtt agcgatcaat aagaaattga
     8341 atttgaccat cgtcttgatc acacatgaaa tgaatgtgat ccgcaaaatc tgcaacaaag
     8401 tcgctgtgat ggagttgggg aaagtcgtag aagaaggaga cgtattgact gtcttcagac
     8461 aaccgaaaaa agaagtaacc aaacgctttg tacaacaaga catcgaacca gaagctgatt
     8521 cgacagaact attagctgaa ctcatcaaag aaaatccaag cggccgtttg gcaactttga
     8581 cctttagcga aagaaatgcc aacgagccag tcatttccca agcgattcga cgctataaca
     8641 tcgatgtcaa cgttgtgtat ggaaagatca aacaagtgaa agaaggttcc tttggttcat
     8701 tgactgtctt gatgactggt gaaaccaaag aattagatca agcagaagtg ttcatcaaag
     8761 aacaaggagt aggaattgag gtgatccatc gtggataaaa gcttttcaga aacatatcta
     8821 gattttaaac aaatcaaccc ggaaacgatg aatcaggcta tccttgatac actatatatg
     8881 acattaatct caatggtctt agtgacgatc atcggattag ttttgggatt gatcctttac
     8941 tctattggac gcaaaaacaa accgtatgct cgaatcattt atggaatcgt atcgatcatc
     9001 agtaatattt tccggtcaac gccatttatg atcctgatgg ttttgatcat tccatttact
     9061 aaagcaatcg taggcactat gctaggtgcg caagcagcca ttccagctct tgttttatcc
     9121 gcagcaccat tttatgcccg tctagtagag attgcttttc gtgaagtcag ccctggtgta
     9181 ctggaagcag cagatgcaat gggagcaagt tactgggaaa tcatctggaa ggtcttgata
     9241 cctgaaagta ttcccgcatt gatttctgga ttaacagtta caacgatctc gatgatcgga
     9301 tttactgcga tggccggcgc gatcggcgca ggtggacttg gaggtcttgc ttggcaggaa
     9361 ggctaccagc gtggtaatct aacagtgaca ttcgttgcga ctttaattat tttactaatc
     9421 gtatttatcg ttcaaggaat cggtgatttc ttaactaaga aaacagacaa aagataatag
     9481 gatatagggg gatgtaggat atgaaaaaga aaatttttgg atttgcagca gcgcttttgc
     9541 tgactgtagg attagcagca tgcggtaaca acagcgacag caaagattca gcaaataaag
     9601 cagacgacac gacattgaaa gtcggcgctt caccgacacc gcacgcagag attttggaac
     9661 atgtgaaacc attattgaaa gatgaaggaa tcgatcttga aatcgtcaaa ttcgatgact
     9721 atgtattacc gaaccaatct ttggaagaag gcgacattga tgccaattac ttccagcata
     9781 ttccttattt gaacaaagaa atcaaagaaa aaggatatga ctttgtcaat gccggtgctg
     9841 tccacatcga accaatgggc ttgtactcta aaaaagtcaa agatgtttcc gaattaaaag
     9901 atggtgcaac aatcattact tcaaattctg aatcagactg gggacgcatc atcacaattc
     9961 tacaagatgc tggcttagtc aaagtcaaag atggagtaga tctggaaaca gcaacattcg
    10021 atgatattga agaaaaccct aagaacttga agttcaacca tacgatcgac ccatcacttt
    10081 tagcttctac gtaccaaaat gatgaaggtg acctagttgc aatcaacgca aacttcgctt
    10141 acggcgcagg gttgaatcca ttagaagatg ccgtattgct tgaaaaggac aactctcctt
    10201 acgtgaatat tattgcgact cgtaaaggtg atgaagacag tgacaaaatc aaaaaactga
    10261 ttgaagtgct tcaccgcaaa gaagtccaag aatggatcac agacaaatgg ggcggctctg
    10321 ttaaaccagt aaaggctgat gcaaaataag gtatggaaca aaaaagatga gaaataagtc
    10381 tgtaatggct t
//
LOCUS       DAZULY010000095        10357 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000095 DAZULY010000000
VERSION     DAZULY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.096"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG81A_001551"
     CDS             6..305
                     /locus_tag="ACG81A_001551"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5014919.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG81A_001552"
     CDS             378..1739
                     /locus_tag="ACG81A_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFS5014920.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG81A_001553"
     CDS             1761..2021
                     /locus_tag="ACG81A_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014921.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG81A_001554"
     CDS             2035..2787
                     /locus_tag="ACG81A_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFS5014922.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG81A_001555"
     CDS             complement(2881..3486)
                     /locus_tag="ACG81A_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5014923.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG81A_001556"
     CDS             complement(3539..4513)
                     /locus_tag="ACG81A_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFS5014924.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG81A_001557"
     CDS             complement(4891..5610)
                     /locus_tag="ACG81A_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5014925.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG81A_001558"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG81A_001558"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFS5014926.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG81A_001559"
     CDS             7161..8558
                     /locus_tag="ACG81A_001559"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5014927.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG81A_001560"
     CDS             8639..9079
                     /locus_tag="ACG81A_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFS5014928.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG81A_001561"
     CDS             9227..9553
                     /locus_tag="ACG81A_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5014929.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG81A_001562"
     CDS             9554..10303
                     /locus_tag="ACG81A_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFS5014930.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3286 a         1825 c         2147 g         3099 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactg
//
LOCUS       DAZULY010000096        10216 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000096 DAZULY010000000
VERSION     DAZULY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.097"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..508
                     /locus_tag="ACG81A_001563"
     CDS             35..508
                     /locus_tag="ACG81A_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFS5014931.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            517..744
                     /locus_tag="ACG81A_001564"
     CDS             517..744
                     /locus_tag="ACG81A_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5014932.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            1380..1751
                     /locus_tag="ACG81A_001565"
     CDS             1380..1751
                     /locus_tag="ACG81A_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014933.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2007..2249)
                     /locus_tag="ACG81A_001566"
     CDS             complement(2007..2249)
                     /locus_tag="ACG81A_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5014934.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(2281..3183)
                     /locus_tag="ACG81A_001567"
     CDS             complement(2281..3183)
                     /locus_tag="ACG81A_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5014935.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3196..3384)
                     /locus_tag="ACG81A_001568"
     CDS             complement(3196..3384)
                     /locus_tag="ACG81A_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS5014936.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(3398..3961)
                     /gene="amaP"
                     /locus_tag="ACG81A_001569"
     CDS             complement(3398..3961)
                     /gene="amaP"
                     /locus_tag="ACG81A_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS5014937.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(3999..4892)
                     /locus_tag="ACG81A_001570"
     CDS             complement(3999..4892)
                     /locus_tag="ACG81A_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5014938.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(4970..5893)
                     /locus_tag="ACG81A_001571"
     CDS             complement(4970..5893)
                     /locus_tag="ACG81A_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS5014939.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(5942..6292)
                     /locus_tag="ACG81A_001572"
     CDS             complement(5942..6292)
                     /locus_tag="ACG81A_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS5014940.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(6325..7227)
                     /locus_tag="ACG81A_001573"
     CDS             complement(6325..7227)
                     /locus_tag="ACG81A_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS5014941.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7220..8077)
                     /locus_tag="ACG81A_001574"
     CDS             complement(7220..8077)
                     /locus_tag="ACG81A_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS5014942.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            8411..9220
                     /locus_tag="ACG81A_001575"
     CDS             8411..9220
                     /locus_tag="ACG81A_001575"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5014943.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9260..9757
                     /locus_tag="ACG81A_001576"
     CDS             9260..9757
                     /locus_tag="ACG81A_001576"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFS5014944.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(10142..10215)
                     /locus_tag="ACG81A_001577"
     tRNA            complement(10142..10215)
                     /locus_tag="ACG81A_001577"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10179..10181),aa:Ile,seq:cat)
BASE COUNT         2949 a         1938 c         1762 g         3567 t
ORIGIN      
        1 tattttagct atcaaagata aaagagggat aaaaatgaaa tttaaaacat tgctattaaa
       61 atttgtcgtt ttaggtataa gtgccgtatt tattttgttc ggcgtacttt tttatactca
      121 gatcgctaca agcgaaaaag gattcaattt tgattggcag accacattat ttgtttctgt
      181 catttacttt gcccttctcc tagggatcgt tattgcttat ttcttattga aactattgaa
      241 aaaaatcgaa gataaccaag ctttttccac tcaaagctta aacatactaa aaaaaatcga
      301 acgaaccatt ttgtttattg gtttcgcctt ttttggctta cttcctaaat ttatgaaagt
      361 agcggattta gacgatgctc caggtttgat gttggtagga atcgctgtcg tattcattcc
      421 ttttgcagta tacacattaa caagcgtgct ggaaaaactg cttgaacatg cgattttact
      481 taagacagat catgatttga cagtgtgagg tgcgacatgg caattatcgt taatttagat
      541 gtagtattag ctcaaaaaaa aatgagtgtg actgaattga gtgagaaagt gggcattacg
      601 atggcaaaca tctctattct aaaaaatgga aaagcaaaag ccatccgttt ttccactctc
      661 gaaaaaatct gccaagtttt ggagtgtcaa ccaggtgatt tgttgaccta taaaaatgac
      721 tcgaatacta taccagaaga ataaaataaa gtagaacaaa cagtaaataa aaaataatct
      781 gaatgataga tgtgtgagac ttttagaagt tatttacgtc tattattcag attttttatt
      841 tcgattgaat accgttttat ctataaataa gatatgtact gactcataca aagggggcct
      901 tttcttctcc atgtactatc acttttcctg atacaactgg tttcctgtta cgttgtagtt
      961 ggatctgctt ttagtataat actttattca ttccaccaga ctgatagcag tttgagcagt
     1021 ctaaataatc atgtagtatt acaggccatt ctgtttcata ccttgaagtg cttaaaactt
     1081 cagctaatgg cccattgtgt taactcttac tccattcttt tttagtaagc tcccatatat
     1141 actgtaaagc cttctatata ttatgtcatg ttgcttttta acatttacta tgtagttttg
     1201 ataaaatgat ttgaataaca gtgatggaca attctttttt atttttaaca ttattttgat
     1261 aataatgatt atttatttaa aaatacatta gataacattt gttacgacac gaaaaagctt
     1321 cacaaacaaa tatcgttatt ttaaaatgag ggcgaactca cacaattagg aggaatagta
     1381 tgccatttat tgtttacttt tttctttcat tactcgtgat ctatattcca ttaccaacat
     1441 ttttcctgct cgtcaatcgt ctagcagagc aacatgatac gattgctgct cttacgaaac
     1501 tttgtttact tctatttgtt tgtctagatt actttacgag ctattattgg ctaaaacaga
     1561 caagagtaaa aaagagatat tatttcttcc ttgtcttttt caatctcttt gagtttctga
     1621 tccattgctt tttacttcat cagtatcata cgatccattt gtggatcatc tgggttttca
     1681 aagtggtcgt attattttgt atgttcacct ttcctatctc aagaaaattt agggaggcat
     1741 tgttttatta aagaagcttc tctgtatcaa taaagacaca tttaaggaaa aacttttaaa
     1801 aatctaattg ggaatttgcc cgcataaata agaggctgtg acaaaagtct tgtcacagcc
     1861 tcttattccg tataaacagt gttcaaaagc ttgcatcttc gataatatgc ccaaccaaat
     1921 gaaaaatatg agaagctatt tttgtttggc tgttcttatt acttgatgaa gaacagcttt
     1981 ttcacgacct ctttttaaaa gtctttttac agttttcgag caaagaatga tacgattagt
     2041 actaaaatga tggcaccaat caatgatggc acgattgcca ttcctgcaat acttggtccc
     2101 caagaaccta ataatgcttg accaagtgct gatccaacta aacctgctac gacatttgca
     2161 ataatcccca tcgaagaacc tttattcgtg attgctcctg caattgctcc gataattcct
     2221 ccgacgataa gtgaccaaat aaatcccatg tttcgttcct cctaatgttt tacttcctta
     2281 ttactgaacc cgtggttctg tattttcttt tactttttgt gcgccgcttc cagctgctga
     2341 ttttacttta tctgtttgat tcgaagcaaa ttcaccggtt tttttcgctg cgtcagttac
     2401 tttgtcttgc accgtttctt gatctttttc aaattcttct tttgttttga tgtccactac
     2461 gttagcgttg acttcgatca cttctaaatg tgtcatattt tttacttctt tgccgattac
     2521 ttcttgcatt tcttcaaaga ttttttcgat atctttgcca tattccacaa tgacatccat
     2581 atcaactgct acttgttttt taccgacttc tgtttcgatc ccagccgtta cattatctgt
     2641 attcacaagt tttcctgcca cattagagaa gaaaccaccg tttgcagata ataaaccgtc
     2701 tacttgttca attgcgattc cgatgatttt ttgcacaact ttatcttcat aattcagctc
     2761 accgcgcatt gaatcttcct gttcagaatc atgctttcct tggttatttg tatgatcaga
     2821 accaagtttt ccttgtccgt ttgtgtggtc agaactaagt ttttcttgtg ctttcgcatg
     2881 gtcagaagga tgtttttgct cttccacagg tttagaagga tcgattgcat catgttgttc
     2941 tggaacttta gattctggag ttcctggatg atgaggtgca tgcttttctg catcatgttt
     3001 cttctcttct actccttgtt gaacgttagg gactactgga tctggatgtg cagatactgg
     3061 tgctgttttg ttcttttcat cgttcacacc atttgattgg tttggtgtcg ttgggtctgg
     3121 atgtgcagac actggttttt ggttgttcat tttattgtta ttatttttgt tattgttatt
     3181 catggatgtt tcctcctaag atttaaaata gtgatctaag attttcgttt gtttcagata
     3241 aaaaccgata gcgacgccga tcgctgccat gatcaagatc aataaggtct tgaaaaagcc
     3301 cacggtgatc cataaaacgg ctaatatcaa tccaagtacc ccaaagatga tagggagctt
     3361 gtatgtcgaa aatagttctt tcatttttgg tcccccccta ttctactcgt ttgcggtcat
     3421 tgcttcgttt agctggttct tcaaagcctg tgtatttcac cgatactttc actggctctt
     3481 ttgttcccaa tatgtgttgt acttcatttt cgatttcttt catcaatgtt cctgttttac
     3541 caatgagtgc tgaagttcgt ttcaactccc cttttacatt gacggcgatc ttgtttttcg
     3601 ttgctttgac atttactttt ggtgaatgga tgaattcatc ttcatgcaag tgagaacgaa
     3661 ccattccttc aattgctttt ttatccaacg tcaactcccc atcaccatgt ttcagtagaa
     3721 agtttccgat tgtttttgga taaaacagga aaacaagtaa cgtgataatt gtcaaaatag
     3781 caaatgcaac ggcaagccaa aatacatatt gttgcacgta tgaattagtg atggaaaaga
     3841 atcgaaactt ctctagctga aaaggcaatg gataggttgc acttaatagt atcgcaaaca
     3901 aaagcacaga tagcaaaagt aatgttccga tcactccgat cgtttttaca cctttattca
     3961 ttttcgcacc tcctcatagg ggtgaaacga tcaacttctt ataagttgat cgcttctcca
     4021 ccagttactc cgtaaatttg ggctgtaaca taacttgctt tattcgatgc taagaaaaca
     4081 taaactggtg ctaattctcc tggctgacct gcacgttcta gtaaactttg ctgaccaaat
     4141 tcagggatcg aatcagaagg ttgtccttcg tctagttgta atggtgtcca gattggacca
     4201 ggtgctacac catttacacg gattccttta ggtgcaagct gttttgctaa accgatcgta
     4261 aagttagcga ttgaagcttt tgttgctgcg taatctaata aatgttcgct tggattgaat
     4321 gcttgaacag aagttgttgt tacgattgct cctcctgctg ggatatgcgg aacagcttct
     4381 ttcactaaag caaacatagc gatgatgttt atcataaatg tgtctttgac ttgctcaagt
     4441 ggtaactcag caatagactg acaagaaatt tgctgtcctg cgtttagtac catcgtatct
     4501 agcccgccaa gttcactcac ggctttatct atgatttctt taggtgcttg ttcgtcacga
     4561 agatcaaaag gtagtaagac aatcttacgt cctgcatctt tcacgtattt tgctacttcc
     4621 tctgcatctt tttcttctcc tgggaagaat tgaagagcga tatctgcccc ttctctagca
     4681 aatgcaatcg ctgctgcacg accgatccct gaatctccac cagtgattag tacacggcgg
     4741 ttttctagtt gattattgcc ttcataggat tcttctccac aatcaggaac aggaatcata
     4801 ttctgctgta aagctggtgt atcctgatct tgtttaggaa atttctccgt atggtacaag
     4861 tttcgcgggt cgattaattt tggttctgtc attgctgtat tcctcctagt ttttaaattg
     4921 tattgccaaa acaaatttct ttgtttgata cctgttttaa atagattctt taatttgtat
     4981 aatctttccg gtataaatgg taacctaaaa ctactgctgc tatcaaagag ccaaagacta
     5041 cgagtataaa gcctagagca gaacttttac ccggtaatcc tcctgtattc attccccaaa
     5101 tccctgcgaa caacgccgga acggaaatga ttaatgcagc tgaatctaga tatttcatga
     5161 tatgatttag attattgtcc atcatcccgt tgaatagatc accaatactc tctaataaat
     5221 ctcgatagat ctcgatcatt ttttctgttt gtcgctgtct caaatggaca tcatagagta
     5281 gctgttcatc gtctaaacgt ttgatgaaag tttcagaatt ccataattga tccattgttt
     5341 cccgttgatc tcttaaggta tgatcgatat atacgatgtc tctttcaaga tctgcctgtt
     5401 tatacagttc ttcattttcc gttgttttac gagctgcttt atccaaagcg tcaattcgct
     5461 ttttcatctc tcttagttct ttcacgtaat gggtgtaaat atctaataat gtatacgtga
     5521 tgatcttttc aaacgagttt aaatgatttc catgctttgt cagcagccga tccacaaatt
     5581 cggtttttct atcgatacaa gtaatcaaga gattatcgga aagtacaaaa gatattggcg
     5641 ttaatttttt ttcgatacga ttatctgact gatctagatt aatcactacc aaagaaaatg
     5701 gatgctctag tttcgtctca taaaggcgct ccatccgaga aacttcttcc ggatattcgg
     5761 ttcctacgaa aatatcatca ggaagctcga attcttcgat tgcttgctca atctcttcgg
     5821 gctttgcact atcaagtacg agccaattat attttttttc atctgtttct attacattct
     5881 tttcttcatc cacaaagtag tatctcaatg tatacctcct ttttggactt tctcgaaact
     5941 attagctgaa gaagcagttc tttaatttga tattataatc gagtactgcg atttcatttt
     6001 ctaatgaaaa gctgtcaagc tgtttgttga agtccatgtt caatcgttta ccgaaataat
     6061 tttgctctaa tttagatgta tagatgtcca ataatggatg ctcgatttta gtcgcatatt
     6121 ctccaaattc cgaaacaaaa ggaatgattt gaaaaaagct gccttttgca aaagcacctt
     6181 tgtcccttag aaatggatcg gaatcattga taagcgcgag tgcgattttt gtattttccc
     6241 caaccttttc aattaacgtc tctactgcat ttttagacat tgtaatttcc attcctactc
     6301 cccctcttca gttgttgtct tctattattt aacaactaca tctgtttttg gttgaacttt
     6361 tgcatatttt ggatatttag ggaatttagc tacacaacgg aaagccgtca tccctgcata
     6421 tttttcgtaa tatttcttcg ttggcccttc aaaatccttg ctccacggtt tcacatgtcc
     6481 tgaaaaatgg ataatgtaag gattgttgcg agtttcttcg tattcacgtt cacctgctgc
     6541 tgttggatgt ttttttgctt ttgccatgat atagccttgt gcattccaac gcggatgaag
     6601 tggtaaccaa cggtcatgta agatagcatt taatgcatct tggtcatgga atcgtaattt
     6661 ttctggatta tgttcgatga aatctaatac tttttgcgta atattttcat ctaaccattt
     6721 tttcacattg atcagcatca aacctgagtt gaaataacgc attgatttag ctgaaatttt
     6781 cattttttcc agtcgttgat ggaacccagc gtcttcaaca gctgctacga ttgaatcacc
     6841 gaagtcaaga cgccataatt tagagatatc ttgtaaagcg atcatgtcgc aatccatata
     6901 taagatacgt tcaacttctg tgccgcggaa caattccgga atcgcaatac gataataagc
     6961 tgtttctgga atacgatcgc tgattaccac atttgtaaag aattctttat tgattttcaa
     7021 gaattccaca tctgagttca ttcgtgcgtt tttcactgag aagcgtaaaa gctgtttact
     7081 ttcaaaatcg atatcatcgt caatcacata gaaccgaaca aatttagacg ggttgcaatt
     7141 atctaatact gagacaaata atgcagctaa atgtggtacg aattttgtgt tacaacttgc
     7201 gactactgct atttcttttt tattcattga tctcgcctac tttcgttaga gtactgtgtg
     7261 ctaatttttt taagtataaa ttggtatacg gatgatcttt caacgtattc caaggtttat
     7321 catgtcctgt aaagtgaacg atcgaaggtt tttcatttcc ttctttatac tgacgttcat
     7381 atttttcatt tggtgctgga tgacgttcaa aaatcagcga agtctgcatg ttccattttg
     7441 gatgaagttg ttcccaatct tcataaagta cagcattcaa agcatcttgg tcgtgataga
     7501 tgattcgatc gccattttca ctgaggtaat ggatagtttt ttctgtaatt tcttttttat
     7561 tccattgatc aatgtcaatc accatcactc ctgaattgaa gtacaagtca tcggaatcga
     7621 ttcctaatct ttccaaagct ttcacttggc ctggatcgat aatagcgccg attgtttttc
     7681 catttaacgg ctcatcatat agttttacga tatcgtctag tactaacaca tctgaatcta
     7741 agtaaaggac tttcttataa tcctctttag ctaataagtt tggtaaagaa attcttaagt
     7801 aagcggtagt tgtgatatgg tcactgacta ggaagtcttc gtagatatct ttttccactg
     7861 tcaggaattg gatacttgca ttacttgaat atttatttac cgtttcttcc aatccttctt
     7921 tactatattc tgatagacca tcgtcaatca cataaaattt tatgcgtctt gttttattgc
     7981 aattttctaa tgcagttgcg atcatcacgc tcaagtaagg agcataattt tcgtctgaag
     8041 cagttaccac aggaactacg ccgtatctct tctccatcaa aatcactcct ttttctaatc
     8101 tttttctaac ctgttctaca tctagaatat tcctcatttg gttttaaagc aaaatatatg
     8161 ctttgttttc attgaatatt ttaaaatttt tttatcacaa aacgcatcgg taagcgctaa
     8221 ctattcgttt ttcatggctt tttattcaaa ggtattgacc aaaaactact atatatagcc
     8281 acctttttct taagaatgtt ttttgaaaaa attcataatg acaaagcaag ttagtttcat
     8341 ttttttaatt tctcatttaa tcttaaggta acaggaaaat agatatgtaa aaagaaagta
     8401 ggaagataaa atgaaactag cagcgattga tttagacggt accttgcttg atcgaaatgg
     8461 aaagatacca gaagaaaata tccaagcact tcggaaattt gatgaaaatg gcggaatcgt
     8521 aaccgtcgct accggaagga acagcatttc tgctaaagat attttttccg aattaggaat
     8581 cggtggctat ttgatttctt ccaatggttc actgattgct gaaatgaagg atggaaaaat
     8641 ggcgaaagtt ctcagacgtt caaaaataga tagcgataca ctgagaaaag ccttcttcta
     8701 tgctcaagaa gcgaaaatat ccatcattgc tagccgagaa acccaagatg atcagctgac
     8761 attcgatgaa tcaaatctag tcaaagatga cccttattat gaacatttca atttacagaa
     8821 ccattctttt gatcaaattt taaatatgtt ggaagatcct tctcttagct atcttaaatt
     8881 agcattaaca gataagagtg aagaaaaact acagaagcta caaagagatc tgcaagcaga
     8941 tggtatccaa tcagtattct ctgatccgca tttcttagaa atcacgccta caggtatcac
     9001 taaagctcat tcacttgttt ttctggcgga ttaccttgga atcgaatcga gtgacatcat
     9061 ggcttttggc gatcaagaaa acgacattgc tatgttgaat tacagcgggt tgagtattgc
     9121 gatggggaat gcacagcgac aagtaaaaga aattgcaaac caagtaacac tgacgaatga
     9181 agaagctggt gttgcttata ttttaaaaaa cttctattaa aaagaataaa atgacgatat
     9241 gaatgtagga ggaaagaaaa tgttagttat tattgatatg caaaatcata ttcttgatcc
     9301 aaacagcgaa ttttatattc cagagtcaga tcaattggca gaacgtattg ctcaaagatt
     9361 agctaaagca cgccaaaata atgaatatat tctcttcaca cgggatatac cagttgaact
     9421 gaaggacaaa gaagaagaac gagaagattt gcaattgatc cctcttcttt ctccacaaga
     9481 aaatgaacga gtgattaaaa agtattactt tacgattcca cctgaaactt tagctgaaat
     9541 ccaggaaact ttatttgaaa gcaaagaaga acaaaaagag atcgaagtag tcgggattga
     9601 aacaaacttg tgtgtactct ctaatctgat aggcctgcag agtgcttttc ctgaagcaga
     9661 cttttttgtt gatccaatgt tagtctccag tagaaaacac ggagaatccg ctttagagct
     9721 gttgaaagat ttcaacgtct ctatcgtcga atcataaaaa tatctatctt catttacgat
     9781 atttaacaca caataaaaaa agcacgcaga actgttttag tcctacgtgc tttttctatc
     9841 aaagtagatt ggctttttta tgacacagag tgaaacggca ggctcacgac ctctttctta
     9901 aatggtgttc aatcagctga ccctcggtat tagcttaaca aacggcaaaa tatgaggaac
     9961 tatttttccg tttgttaagc taatactcag gtcaaaacgc tgatttcaca acctcttcct
    10021 taaacggtat tcaaaagctt gcatctgcag taataagaac agccaagtaa aaacataaga
    10081 agctgttttt acttggctgt tcttattact tgatgcaggg cagctttttc acaacctctt
    10141 atgtaccttg taggactcga acctacgacc ggacggttat gagccgtctg ctctaaccaa
    10201 ctgagctaaa ggtaca
//
LOCUS       DAZULY010000097        10182 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000097 DAZULY010000000
VERSION     DAZULY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.098"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            109..615
                     /locus_tag="ACG81A_001578"
     CDS             109..615
                     /locus_tag="ACG81A_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFS5014945.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            632..1531
                     /locus_tag="ACG81A_001579"
     CDS             632..1531
                     /locus_tag="ACG81A_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFS5014946.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1618..2502
                     /locus_tag="ACG81A_001580"
     CDS             1618..2502
                     /locus_tag="ACG81A_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS5014947.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2694..2765)
                     /locus_tag="ACG81A_001581"
     tRNA            complement(2694..2765)
                     /locus_tag="ACG81A_001581"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2730..2732),aa:Arg,seq:ccg)
     gene            2825..4159
                     /gene="rpoN"
                     /locus_tag="ACG81A_001582"
     CDS             2825..4159
                     /gene="rpoN"
                     /locus_tag="ACG81A_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFS5014948.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4308..5399
                     /locus_tag="ACG81A_001583"
     CDS             4308..5399
                     /locus_tag="ACG81A_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFS5014949.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5440..6441
                     /gene="gap"
                     /locus_tag="ACG81A_001584"
     CDS             5440..6441
                     /gene="gap"
                     /locus_tag="ACG81A_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS5014950.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6551..7741
                     /gene="pgk"
                     /locus_tag="ACG81A_001585"
     CDS             6551..7741
                     /gene="pgk"
                     /locus_tag="ACG81A_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFS5014951.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7845..8600
                     /gene="tpiA"
                     /locus_tag="ACG81A_001586"
     CDS             7845..8600
                     /gene="tpiA"
                     /locus_tag="ACG81A_001586"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFS5014952.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8716..10014
                     /gene="eno"
                     /locus_tag="ACG81A_001587"
     CDS             8716..10014
                     /gene="eno"
                     /locus_tag="ACG81A_001587"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFS5014953.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3563 a         1883 c         2111 g         2625 t
ORIGIN      
        1 cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa attgaaactt
       61 ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat gatttttact
      121 ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg actcatcgaa
      181 actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa agaagaggcc
      241 ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa agaaaaagag
      301 atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt agatagttgg
      361 cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc ccaacagtta
      421 cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga cagtccggaa
      481 aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat tgcgactatc
      541 ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa tgctttacgt
      601 caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa atttaaaaaa
      661 actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg agatccgcca
      721 gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga gacgtctcca
      781 agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc tttttaatag
      841 tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg aattgatctt
      901 aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga agatcaatac
      961 acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact ttcgcgaagg
     1021 acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg atcattacca
     1081 agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga ttgaaaggca
     1141 gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa tcgacaagaa
     1201 aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc tatctgtttt
     1261 gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat ctaagtacta
     1321 ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg ttacgcctac
     1381 acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc aatacgaaat
     1441 tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga caaaaacgat
     1501 tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact agcagtttca
     1561 attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag agatatagtg
     1621 aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt tttcgaaatt
     1681 acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc ggagcattcg
     1741 tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac tgaaacagct
     1801 aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc atcacaatca
     1861 acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc aatcagcttt
     1921 gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa agaaaaaaac
     1981 gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga aatcgtgaag
     2041 cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca gaatctggcc
     2101 actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga gatcaaaaaa
     2161 gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac gaatgtagga
     2221 gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga ttggaagaca
     2281 agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg ggcaattatt
     2341 ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat cgattatctg
     2401 aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc aacggctgtc
     2461 gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca agtatcttta
     2521 ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc aaaggcttaa
     2581 caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg gacagttttc
     2641 acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta gaacgcgtcc
     2701 tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca ctacactaag
     2761 gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg gattggacaa
     2821 aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga aactagccat
     2881 gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg agctgttggc
     2941 attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc cggaatggca
     3001 gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa attacctaaa
     3061 tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag tccatttgaa
     3121 ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca tcgatttgaa
     3181 cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta cgcctatcca
     3241 aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag gtgcacgtag
     3301 tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg aattagctta
     3361 tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag aaatcgcaca
     3421 acgtttttct gttgatctac atgctgttca gcagatattc gactatttac aaacgttgag
     3481 tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc ctgatgtccg
     3541 ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa atcagccaaa
     3601 tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata aagaaactga
     3661 aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga tcctacaacg
     3721 tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag ctttctttat
     3781 cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg agatagatgt
     3841 gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag acttcggtgt
     3901 atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag aacagacaga
     3961 agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc aagaagacaa
     4021 aaacaagccg ttgtctgacc agaaattggt tgaattgctg aaaaaagatg agatagcgat
     4081 ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt catcaaaaag
     4141 aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact ttttctagtc
     4201 tttttagttg aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt tgagatttcc
     4261 ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg ggtcgtttta
     4321 agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat gaaataaaaa
     4381 tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat aaaattttac
     4441 gaaatatcta ttggatgcag ccgattggac gtcgaagttt atcggaaagt atggggatca
     4501 cagaacgagt attgcgaaca gaaacagatc ttttgaaaaa tctgaatttg atcgacacct
     4561 caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc cttgaaaact
     4621 tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa tattttggta
     4681 accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg gctgatgcat
     4741 tcggcgaagc gctgagcgaa gccctagacc gactacttcc agaaggcgag aatatcatcg
     4801 ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat ctggaaaata
     4861 agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg ataactgtac
     4921 aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac cgtgcacttt
     4981 atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag ccatctatcc
     5041 aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt gggcgtgcat
     5101 tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg aaacaagcaa
     5161 atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc gtttataagg
     5221 tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc atggcaatcg
     5281 cgggcggaaa atcaaaagcc aaagcaattc gagcttatat gaaaaatgca ccaaaacaaa
     5341 cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta accctttaaa
     5401 ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa agtaggtatt
     5461 aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt agatggaatc
     5521 gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt gttaaaatac
     5581 gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc attcaacgta
     5641 aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc atggggcgaa
     5701 ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc agcagctgaa
     5761 aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg aaacgatgtt
     5821 ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac agttatctca
     5881 ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa cgacaaattt
     5941 ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca aatgactcta
     6001 gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc aaacatcgtt
     6061 cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcctgaatt gaacggtaaa
     6121 ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga attagtaaca
     6181 gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga agcttcaaac
     6241 gaatcttacg gatacaacac agacgaaatc gtttcttcag atatcgttgg tatgacttac
     6301 ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa acaattagtt
     6361 aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt acgtacttta
     6421 gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata gacatgtaca
     6481 tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag tttattcagg
     6541 aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca aaaaagttct
     6601 tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg acactcgtat
     6661 caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag caatcctttt
     6721 ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt tagctccagt
     6781 tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg aaactcgcgg
     6841 agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg tattcgaaaa
     6901 tacacgattt gaagatattg acggtaaaaa agaaagcaaa aatgatacag aactaggtaa
     6961 atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag ctcaccgtgc
     7021 acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg gattcttgat
     7081 ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgac caatgattgc
     7141 aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact tgattcctaa
     7201 agcagataaa atcttgatcg gtggcggaat gacttacaca ttctacgaag caaaaggaat
     7261 caagatcggt aactctctag tagaagctga taaagtggaa ctagcaaaag aattaatcga
     7321 aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag aattttcaaa
     7381 tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg ctttagacat
     7441 cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa cagttgtttg
     7501 gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa tcggtgtttg
     7561 tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag actcagccgc
     7621 agcagcagaa caattaggat ttgctgacaa attcacacac atctcaacag gtggcggcgc
     7681 aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca acgataaata
     7741 aacgaccaag tttgctcaat tctgacgagt aatcgatcaa aaggtgaagg gagttttcat
     7801 gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt aaacctatta
     7861 tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt gctgaagcag
     7921 taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct cctgcattat
     7981 tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca gcacaaaact
     8041 gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct cttgctgatc
     8101 taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc catgaaacag
     8161 acgaagacat caataaaaaa gcgaaagcta tctttgcaaa tggtatgaca ccaatcctat
     8221 gctgtggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg atcgaaggac
     8281 aaattacagc tggtttagct gatctttcag ctgaacaagt gtcaaacatg gttattgctt
     8341 atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc gctgacgaaa
     8401 tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta tcagaagcag
     8461 ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac atggcaaaag
     8521 aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca ttcttgtcat
     8581 tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa tgtttgcact
     8641 aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa aacaaactca
     8701 aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat cttagactca
     8761 cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt tggccgcgga
     8821 atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg tgacggagac
     8881 aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa caacatcatt
     8941 gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa agctatgatc
     9001 gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt aggtgtttct
     9061 atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca ctacctaggc
     9121 ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg cggatcacat
     9181 gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc tccaacattc
     9241 aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat cttgaaagct
     9301 cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct tggttcaaac
     9361 gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt acctggtaaa
     9421 gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga aaaaggtgtt
     9481 tacgtactag ctgattcagg cgaaggcgaa aaaacaactg acgaaatgat caaattctac
     9541 gaagaattag tttctaaata tccaatcatc tcaatcgaag acggattaga cgaaaacgac
     9601 tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt agttggtgac
     9661 gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg aattgctaac
     9721 tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga agctatcgaa
     9781 atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga aacagaagat
     9841 tcaacaatct ctgatatcgc tgttgcaaca aacgctggcc aaatcaaaac tggttctcta
     9901 tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga ccaacttggt
     9961 gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa ataattcttt
    10021 tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac ttagttaagt
    10081 aatcgaactc tctacttgct gaaatatcgg ctctttgtca ataaggactg atgagttgtg
    10141 caaaatcaaa tctgagtcag aatgaaccac attctggctc ag
//
LOCUS       DAZULY010000098        10044 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000098 DAZULY010000000
VERSION     DAZULY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.099"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            122..352
                     /locus_tag="ACG81A_001588"
     CDS             122..352
                     /locus_tag="ACG81A_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFS5014954.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            449..1750
                     /gene="murA"
                     /locus_tag="ACG81A_001589"
     CDS             449..1750
                     /gene="murA"
                     /locus_tag="ACG81A_001589"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS5014955.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1763..1939
                     /locus_tag="ACG81A_001590"
     CDS             1763..1939
                     /locus_tag="ACG81A_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS5014956.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2074..2370
                     /gene="yidD"
                     /locus_tag="ACG81A_001591"
     CDS             2074..2370
                     /gene="yidD"
                     /locus_tag="ACG81A_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFS5014957.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2434..2901
                     /locus_tag="ACG81A_001592"
     CDS             2434..2901
                     /locus_tag="ACG81A_001592"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5014958.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            2986..3870
                     /locus_tag="ACG81A_001593"
     CDS             2986..3870
                     /locus_tag="ACG81A_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5014959.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            3984..4220
                     /locus_tag="ACG81A_001594"
     CDS             3984..4220
                     /locus_tag="ACG81A_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFS5014960.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4308..4388
                     /locus_tag="ACG81A_001595"
     tRNA            4308..4388
                     /locus_tag="ACG81A_001595"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4342..4344,aa:Tyr,seq:gta)
     gene            4395..4466
                     /locus_tag="ACG81A_001596"
     tRNA            4395..4466
                     /locus_tag="ACG81A_001596"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4427..4429,aa:Gln,seq:ttg)
     gene            4591..4761
                     /locus_tag="ACG81A_001597"
     CDS             4591..4761
                     /locus_tag="ACG81A_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014961.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            4981..5688
                     /locus_tag="ACG81A_001598"
     CDS             4981..5688
                     /locus_tag="ACG81A_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5014962.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5748..6392)
                     /locus_tag="ACG81A_001599"
     CDS             complement(5748..6392)
                     /locus_tag="ACG81A_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFS5014963.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6519..6806
                     /locus_tag="ACG81A_001600"
     CDS             6519..6806
                     /locus_tag="ACG81A_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5014964.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6914..7234
                     /locus_tag="ACG81A_001601"
     CDS             6914..7234
                     /locus_tag="ACG81A_001601"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5014965.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7315..8628
                     /locus_tag="ACG81A_001602"
     CDS             7315..8628
                     /locus_tag="ACG81A_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS5014966.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8625..9344
                     /locus_tag="ACG81A_001603"
     CDS             8625..9344
                     /locus_tag="ACG81A_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFS5014967.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9472..10023
                     /gene="raiA"
                     /locus_tag="ACG81A_001604"
     CDS             9472..10023
                     /gene="raiA"
                     /locus_tag="ACG81A_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFS5014968.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3353 a         1661 c         2147 g         2883 t
ORIGIN      
        1 tttcctaaaa aatgtaaggg gattcctata tttttaagta tacttgtttt tccttttata
       61 cattttatgg tattatcatt ttattagtta ttataaaaca atcgttagaa aggacgtcat
      121 tatgcaagta tacggaattg atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta
      181 cttagccttc tggtctttac gttcgttacg cattgaaact tttttcagac caatgcataa
      241 tacgcaaatt agaatggcaa tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa
      301 ttttttctta gaagttatcg ctttatgccg aaatcttttc gttagcctgt aaattttaaa
      361 aaaaggtact attttaaaag caaggatgtt ataatagaaa agattttgag taagtgtatg
      421 ctcattggaa ttttttggag ggaacaacat ggaagagatc atcgtaagag gtggcaatca
      481 attaaatgga acagtaagaa ttgaaggagc taaaaatgct gttttgccta ttttagctgc
      541 tagtttgtta gcagaagaag gtattacgac attagacaac gttccgattc tttctgatgt
      601 cttcactatg aatcaagtga ttcgtcactt aaacgtagat gtagattttg atgaacaaaa
      661 gaatcaagta acaatcgatg cttcaagaca attagaaatc gaagcgcctt atgagtatgt
      721 tagtcaaatg cgtgcttcaa tcgttgtgat gggtccgcta cttgccagaa atggacatgc
      781 aaaagtagct atgcctggtg gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa
      841 aggatttcaa gctttaggag caaaaattat tcaaaaaaac ggctatatcg aagcaattgc
      901 ggatgaattg atcggtaaca cgatttattt agatttccct agtgtaggtg ctactcaaaa
      961 tatcatgatg gctgccgtaa aagcaaaagg aacaaccatc attgaaaacg tagctcgcga
     1021 accagaaatc gttgatttag caaacatttt gaataaaatg ggcgctcaag tctatggagc
     1081 tggtactgaa acaatgcgta ttgaaggcgt ggatcattta catgcagtca accactcaat
     1141 cgtacaagat cgtatcgaag caggtacctt tatggttgct gctgcaatga cacaaggcaa
     1201 tgtgctgatt gcagatgcta tctctgaaca taaccgccca ttgatttcta aattgatcga
     1261 aatgggtgca gaaatcattg aagaagaagg cggagtccgt gtcatcggac caaaacatat
     1321 cttacctaca gatgtaaaaa cgatgcctca tcctggtttc cctacagata tgcaagctca
     1381 aatgacagct atccaattag ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga
     1441 aaatcgtttc caacatttag aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg
     1501 aaatgtagca atcatggatg gaaaccatga attgcaaggt gcagaagtat atgcaacaga
     1561 cttgcgggca gcagcagcat tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt
     1621 gcgtaacttg aattatctag accgtggata ttataatttc catatcaaac tacagcaatt
     1681 aggtgcagac gttgaacgtg tagacatgga tcaaacttct gctgaaaaaa cagcacaaac
     1741 gattgcttaa ggaagtggct gaatgagtaa ccaacgatat atcttattaa ctttgataaa
     1801 gattttagtg gtgatcttat tgcttatcct tctttttgtg gccggaacga tgatcggata
     1861 tggtgtcatc ggtggcggca atccatttaa agtattccag ccaagtttgt ggattcatat
     1921 tcgagacttt tttcattgat aaataagaat cacagtggtt ctagtcgata tgaagtgagc
     1981 catgaagcag aacatgacaa aagaaaacag aagggaacgt tgacgttagt cgcgatccct
     2041 ttttttagta aaagaaaaga aggaatctaa ttgatgaaga agatattgat ttatttagtt
     2101 agaggatatc agcgctttat ttcgcctgca tttccaccta gctgccgtta ttacccaacc
     2161 tgttccaatt atatgataaa agcaattcaa acccatggtg cactaaaagg gacggtaatg
     2221 gggactgcac ggatcttacg ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca
     2281 ttgacattcc aattaacgcg aaataagaaa gataaaaaag aaccagatta taaaaaggtt
     2341 gtgaaaaata cgagtagcac tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa
     2401 caactccgca actacttata tgggggacaa aaaatgatcg aagaactaaa caagaaagat
     2461 ttaccatggt ccttgttatt agatgcagat cctgataaag aaaaagttca agtatatgtt
     2521 tccagaggga gtggctggat atggaaagaa aaagaaaaaa caataggtgt actgatctat
     2581 gtggcaaggg aaaaagaata cgaaatcgtc aatgtagcag tagccccaac gtatcaagga
     2641 aaggggattg gcggaaaatt acttgagaca gcttttcaga agctttcaca actcgcttcc
     2701 agtcaaactc agatcatcat acgaaccggc agtactagca gtgcggcgtt acatctctat
     2761 caaaaaatgg gctatgtgga gattggtcga gtcaaagact attttatcca tcattactct
     2821 gagcccattt ttgaaaatgg agatcaatta agggatcaag taattttagc aattcactta
     2881 cctaaaaata gctttaacta ataaactttc aattcgagaa aatgctttca aatgctaaat
     2941 agatacatta caatagcttt agcaaacaaa aaggagaata tttttatggg aatattagtt
     3001 tttgattttg gtggatcggc tgtcaaatat ggttgttggg atggcaaaga gattaaaggt
     3061 aagggacaat ttgcaacgcc tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt
     3121 tataaaaaaa tactcgtttc ttttgaagtc gaaggaattg gaatcagctc tcctggagta
     3181 gtgaataatg agaaacaggt gattgaggga atcagtgcga ttccttatat tcatggattt
     3241 aatattttcc gagatttgga aactctgttt caactcccag taacgattga aaatgacgcc
     3301 aactgtgccg gaatggctga attttatgaa ggtgctgccc gtgattatca agatgttgct
     3361 tttgttgtgg caggaactgg gattggcggg gcgttgttca cgaaaggaca aatcaataaa
     3421 ggggcacatc tttatggtgg agaatttggg ttgatgttct taaatggcga taagacattc
     3481 agtaaacttg gtacagcggt acagatggct tggcgttatt gtgaacgcaa aggtctcgat
     3541 aaaaattctt ataccggaaa agatgtcttt gaattagcag aaactggaga tgcaatagca
     3601 aaagaagaag ttgagagttt ttatacgtat cttacaaagg gattattcag tattcaattt
     3661 tcgtttgatc cagaagtgat cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt
     3721 gatgagatca aaagccgaat gaagaagctg acagaacaat tcgatctgca tgatttcgaa
     3781 ccgcagattt tattatgtga atacagaaac gatgctaatc ttgtaggtgc agcagcaaac
     3841 tatctcgctg tacgtcaagg ggagaattaa gagctttaga gaaggtgcag cttgcataaa
     3901 agaagtgaat gaaatgaata ggagattttt acctaaggaa tgttttattt gctgcagtat
     3961 gataaaatat ctgagaggtg aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta
     4021 tagaagaagt gaaaaaagat atcaaaggta agaattatga agtcactcag ttatttatag
     4081 ggaaaaaaat gattggtgag atcttagcct atgggccaaa agaatatgaa attttctttg
     4141 gtgaagaaga ttttggaaaa gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac
     4201 actggaatct gcacgaataa aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat
     4261 ctggaaaatt aggtattata actaagtcag gttgatacaa acctttcgga ggagtagcga
     4321 agtggctaaa cgcgacggac tgtaaatccg ttccttaggg ttcagtggtt cgaatccact
     4381 ctcctccatt tcattggggt atagccaagc ggtaaggcaa gggactttga ctccctcatg
     4441 cgttggttcg aatccagcta ccccagtata ccacaatcgt aagctctcgt tagttcgaga
     4501 gctctttttt ttgctttgct accttgttcc ttgaagattc tgctgattta agatagagtt
     4561 aagttaacta caagaattgg agggatccac atgaaatgga aagaaaaagt agacgaagca
     4621 gctgaaaagt tatatgattt ggtcaaatca gaaaaataca atattgaagt gaatattccg
     4681 aaaaaaggcg gaaaagcggt ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa
     4741 tggatggcta aaaaccgcta ataataacaa aagatccata agtcggcatt ttcatcgatt
     4801 ttagggtctt ttgttatttt ctaggttgaa catatctaaa aaattgtttc tgacaatagg
     4861 agtaggaaga acgatgtgtg tctaaatcct ctgttgatgg atagaaacct ttatcttttt
     4921 cagttgtgaa tcaggtatat accagttata atagaagaaa cgaaaggacg ggtgtacgtt
     4981 atgtcgaatc aattacctgt atatatacag attcatgacc aattaaaaaa tgaaatcgaa
     5041 caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa gagaattagc agtgaaattc
     5101 aatgtcagcc gtatgacgtt gagacaagca attcaaaact tggcagatga aggaatctta
     5161 gaaagaaaga tcggttcggg tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct
     5221 ggtgcaacaa gttttacaga aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca
     5281 atctcttatt tcttgacttc gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa
     5341 gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt
     5401 gaagttgcca gtgtgccaca aaagctgatc gatggctaca gcaaagcaga gattactgaa
     5461 tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg
     5521 attactgctg tgttggcttc tgagaaaatt gctgactatt tagaggtaaa acgaggagat
     5581 gcagttcttc gtttgcgcca gatttcttat tttgaagacg gtacgccgtt tgagtatgtg
     5641 cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg aaaaataaaa aacaggtgat
     5701 ccctgattcc ttacttagaa ataaaagaga atcagaatca cctgttttta tatatgatca
     5761 gttaccagtt gctcgtgata agaaacagga ccctcttcaa attggacttg tccgttcagc
     5821 agctcaatga tggattcttt aaagtggtca gcactttttt catctacagc acagatgacg
     5881 ctaacttgat cagtatattg gatttctttt ataaaaaaat gttggagttc aagataattc
     5941 tgtaattttc ctagttgcgg atacgtgatc atcacagaga tctcctgttg caaggtacct
     6001 tctacgattc caatgtcaga caatgcttga gagacagcat gagtgtatgc tcgtattaat
     6061 ccgcctgtgc cgagtttcgt tcctccaaaa tatcgagtga ctactgcagc tacattgatc
     6121 agttgatttt ttttgaggac ttccaacatc ggtacgccag ccgttccact tggttctcca
     6181 tcatcgctgc ttcgctggat ttcatttttt tctccaatga tgaaggcgct gcagttatgg
     6241 ttcgctttcc aatgttcttt ttttatttct tgaatgaaac tttttgcttg gtcctctgat
     6301 tctattcgtc taagagaaca gataaagcgt gattttttga tctcaatctc actttgtcca
     6361 ttttctttta ttgtacgata gcgctcgatc atcttgtcac ctcttttttt cattttcttt
     6421 attcagtata aataaagttc taggttgttt caacactgta acggttgcgc aatcgctgta
     6481 gtatgcgata ataaagacaa aggtaggtgg atctggggat gcaggaacga atgaaaaagt
     6541 acgatgcaat tacgcattat ttgaagaaca atggcggctc tcaagtaacg ttgaccttta
     6601 cccaatttga cgaactgctg tttcccagta acggacttcc caaaacagca agggaaagta
     6661 cagattggtg ggcaaatgat tataaacacc ctgaaaaagg tgcctatggc tggataaatg
     6721 caggctatga agtagttgtc atcaatttgg acaaagaata tgtcgtgttc aataaattag
     6781 tgaaatctag ttggctattt gattaaataa accgcgttta gaaaacgttg acaaatttct
     6841 ttctttttat ataatagagt aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa
     6901 aggagagaaa agtatggcta aaaaaacaat catgcttgta tgttcggctg gtatgagcac
     6961 aagtttacta gtaacaaaaa tgcaaaaagc tgctgaagca aaaggaatcg actcggatat
     7021 tttcgctgtg tccgcttctg acgcagataa caatcttgca aataaagatg tagatgtact
     7081 gttattagga cctcaagttc gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa
     7141 aggcattcct ttagacgtca tcaatatggc tgattatggt atgatgaatg gcgaaaaagt
     7201 gttagatcaa gcaatcacgc tcataggtga ataaatagaa cagtataact atcgaacaag
     7261 cgatccaaaa aatagatcgc ttgttttttc gtatattttt ttagggcgaa taatatgaat
     7321 catttttggg gtagaaggct tctgacgaga gatattaaag caatagactg tgaggaggta
     7381 aatttaccaa attatgagaa gatcgcagca gtggaacgtt tgggcaactg gattctttgt
     7441 catcgctgtg gaatgaggac gtccgtcatt gatggacagc ttgcagatta cggttatttt
     7501 tgtacacatt gtctatattt agggcgatgc gacactcaac aagatttata tttgtttgat
     7561 cagcctgaag caaaacctcg agaagtcgtc tttttttgga caggacagct tactgaaaaa
     7621 caaacggaaa tcgcaaagag aatactttgc cattcagaaa aaagacatca tcttatttgg
     7681 gctgtaacag gtgcagggaa aacagagatg ctttatccta ttttagtgga aactttaaaa
     7741 gctggaggaa gagtagcgat ttgtacaccg cgagtcgatg tttgtaatga attgttttta
     7801 cgctatcgtt atgtttttcc agaagaaaaa atcacgttgc tccatgggaa tacagcaacg
     7861 gcttatacct ttacttcttt tgttatctgt acgattcatc agctataccg tttttatcgg
     7921 tcctttgatt taatagtgat cgatgagatc gatgcgtttc cttataacgg ggatgcagga
     7981 ttagctcatg ctgtgcaaac agcgattaaa ccagacggac gattcatcta cttaagtgcc
     8041 acacctgata aaaagttact aaaggaaatc aagcagacgt ttgacatcca caaacttgct
     8101 ttgcgctttc atcggcgttt acttcctgaa cccgtcttgg ttttcatgaa taattggcga
     8161 caaaaatgca agaacaagaa gaagataaaa tctttggtgc ggatcattca tcaattaatt
     8221 aaagaaaacc atatcctttt attttgtcca agtatcacga tgatgcatcg tttgaaagcc
     8281 acacttcaag aggctcttcc gagatatgag atcacagatg tttcttcaag agacaatcag
     8341 cgggcggaaa aagtccagaa aatgcgagaa gcaaaatatg acatactgtt aaccacgatg
     8401 atccttgaac gtggagtcac atttgaaaga atatccgtta tcgttttagg agcggatcat
     8461 ccggttttta ctaaatcttc tttggtacaa atcgcaggaa gagccgatcg taaagggcct
     8521 tatacgaaca gtcaagtata ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct
     8581 tgtcaggaaa tcaaagaaat gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct
     8641 gcagtaaaga tatcgtccaa aatttaacat tcagcgagat tattggtgtt tcgaaataca
     8701 gattacctca gacttgttca gtttgtcgcg cacaatttca tttattggaa acaagaggct
     8761 gttctggctg tcaaaaacca atccatttca atctagagag tacgaaaaag aaagtaaagt
     8821 ctattcccgt ttactgcagt gactgtcaag aatggaagaa gaaatatccg acctatgatt
     8881 ttcagcaccg tgcctttttt gtgtacgatg aaaatatgaa ggcctggctc aatcaataca
     8941 agtttatggg agatatccga ctagcaggca cctttgcata taaatggacg cagataaaaa
     9001 aagaataccc gacatacatc tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa
     9061 gaggattcaa tcaagtaagc gagatgttga aagcagcaag aattcctttt cagatgctac
     9121 tcaaaagaga gaagcatctg ctggctcaag caaaaaacac gcgaaaggaa agattggaga
     9181 tgcctcaacc ttttgaactt gcagtcacct tttctaagat acaaaataaa aaaatattga
     9241 ttattgatga tgtctataca acaggacaga ctcttttcca cgcagcagat tgtttgcagg
     9301 tcgcacagcc gaaaatcatc cgaaccattt ctttagcaag atgaattaga tgtggaaatt
     9361 tgatagcgat ttaatttgca aataacctag agttcgatat aatagaatta agaagaggga
     9421 aagagtggtc cttctccttc ttgattgtgg tagacgaaag gggtaatttt tatgtttaga
     9481 tataatgtac gcggcgaaaa tatcgaagta actgaagcga ttcgcgacta cgttgagaaa
     9541 aaagtaggca aactagaacg ctatttcagt gacgcacccg atgcaacggc gcatgtgaac
     9601 ttaaaagttt atacagaaaa aacagcaaaa gtggaagtaa cgatcccatt accttatctt
     9661 gtattacgtg ccgaagaaac ttctccagat ttatacgcaa gtatcgattt ggttgttgac
     9721 aaattggaac gccaaatccg caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg
     9781 ggcatgaata ctgcagatgc tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat
     9841 tctgaattag atatcgtacg tacgaaacgc ctttcattga aaccaatgga tagtgaagaa
     9901 gcagtcttac aaatgaacat gctgggacat aacttcttta tttttgaaga tgcagaaaca
     9961 aatggtacaa gtatcgttta ccgccgtaaa gatggaaaat acggactgat tgaaacagac
    10021 tgatcagcct tacttaaata acaa
//
LOCUS       DAZULY010000099        10025 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000099 DAZULY010000000
VERSION     DAZULY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.100"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..221)
                     /locus_tag="ACG81A_001605"
     CDS             complement(24..221)
                     /locus_tag="ACG81A_001605"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS5014969.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(367..1245)
                     /locus_tag="ACG81A_001606"
     CDS             complement(367..1245)
                     /locus_tag="ACG81A_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS5014970.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1284..2402)
                     /locus_tag="ACG81A_001607"
     CDS             complement(1284..2402)
                     /locus_tag="ACG81A_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFS5014971.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2399..4498)
                     /locus_tag="ACG81A_001608"
     CDS             complement(2399..4498)
                     /locus_tag="ACG81A_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFS5014972.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4549..5370)
                     /locus_tag="ACG81A_001609"
     CDS             complement(4549..5370)
                     /locus_tag="ACG81A_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5014973.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5367..6176)
                     /locus_tag="ACG81A_001610"
     CDS             complement(5367..6176)
                     /locus_tag="ACG81A_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5014974.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6197..6691)
                     /locus_tag="ACG81A_001611"
     CDS             complement(6197..6691)
                     /locus_tag="ACG81A_001611"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5014975.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6703..7203)
                     /locus_tag="ACG81A_001612"
     CDS             complement(6703..7203)
                     /locus_tag="ACG81A_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5014976.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(7268..9964)
                     /locus_tag="ACG81A_001613"
     CDS             complement(7268..9964)
                     /locus_tag="ACG81A_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5014977.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         3047 a         2260 c         1654 g         3064 t
ORIGIN      
        1 ttttatacgg ctgaaaaaat ctatcaccaa aaaaattcaa acattaagcc aaggattatg
       61 aagtcaacat ttcgaaaaat gaacatacat tggtaagcaa ctataactat taccacagac
      121 ataaagtaac acaatatatt actatttaag tgtacgccac cagtgaatat cctcaaggta
      181 tacagcgcac aacaaaaaga aaaaaggaaa cgcagaagca tttcctttca gtgatttaat
      241 gaaaacaaca cgatttgact tagagccttt ttatttgaat tcattttcgc tttatccaac
      301 ttcatttgat catttgatta ctcggttaaa tacttttgac atgagtatca atgaaacatt
      361 cattaattat ggttgcttat ttcttaccgc ttttctggcc aatgctaatc cgccgattat
      421 tccggcatta tcacctaatt cgggcgtaat aatgtagcta tcaattggtg gtatttcaac
      481 atatccattt ataagctcaa caaatttatc ctgaactttt ttcttcatgt gttcttgttt
      541 cattacacca ccgccaaaaa taattctatc tggcgaaaac atcagtgttg tattgtatgc
      601 acattgggca aggtaaaagg cttcaatctg ccaaaacgaa tcgtcagcca ataaattctg
      661 gccttttaca cccaaccgtt tttcaatcgc tggtcctgct gccatcccct ctaaacaatc
      721 ttggtggaag ggacagtttc ctgaaaaatt gtcctttgga tgtcgcttga tcaacatatg
      781 ccccatttcc ggatgactga acccttccac gaaagtccca ttgaacaagg caccacctcc
      841 gatacctgtt cccacagtga agtatgcaac acctgctaac cctttgccag aaccagcaac
      901 atattcacca tagcaagccg cattgacatc tgttgtccat tcaatcggaa tattgaaata
      961 ttgctttagc gttcctacaa aattgaaatt ttgccaagca agttttggtg ttgatgtgat
     1021 atgaccgaaa ctcgttgatt tgcattgaat atcaattgga ccaaagcagc cgaccccgat
     1081 gcctgccaat tgttccttaa aaggtttaaa aaaatcaatg acctgcttca ttgtttcaac
     1141 tggagttgtt gtgggaaaac tgactctttc caaaatcgtt aattcctcag taccgactgc
     1201 acagacaaat ttagtccctc cagcttcaat tgccccatat atcatcatag gttcctccct
     1261 tcgaatatgt gaaaaacact tcattaacgc ggcaagcgtt ctcgatcagt gaaatgacgt
     1321 ttagtatttc ctgaagcttc tgttgaatac cagtaagcga cactagcaac atcgtcttgc
     1381 cgctcaaata agcgaacatc atcgttgccg atttgttgta aagtgaccga taaatcttcg
     1441 gtaaaacaga tggggtcttg aagatgccaa cgataaagac catgtcgtgg cattgcgtcg
     1501 tcaccaaagg catgaacggc attcaatttt cccgtttcaa aacgatctct tgtggcatct
     1561 ctatttgatt ggtaaggata ccctaaaaag gctgttgaat agtttttcgc atgtggacgt
     1621 ccgaactcat cgtactcatg aaaggcccac gccccgccaa aataatcttc tgaacctgtt
     1681 gaggttacgg tcggatactc actatcccca tcaatgtaga atttaaactc gccttcgccc
     1741 caccaataac gttctaggca ggtaagcgca aggtaagtac ccacgtaata gccttgaccc
     1801 tttaatcgat caattaactt ataatcctcc tgtaatttgg taatgcgttc ccgatgccaa
     1861 tttgcgtgaa aatataggct attttcttcc aattcgtcca ccaaagcgta atttatcgta
     1921 taaaaaaatg aggacatatc cttcggatgt aaattctcga tcgtaatttt agcggatgtt
     1981 tggaaaggca tttcgaaata ggaattaaag ccccctgtag gattaaccac tatcggcaac
     2041 gagttcacct cgcaacgctc tccaaaccca ttacaaaaga aatcccctaa aggactttca
     2101 acagcagggc tatccgaccc atcccaataa attcttaaga ttaaatcacg taaaacaaaa
     2161 ctgccaaatt ctgttcgatc acgaatcgtc atccacatat gacgaattat tcccgttcca
     2221 gtaatatccg caatcacagt ttcttcacca gaagtcagcg gaattgcgcc acgacctttg
     2281 cggcttggtc ctagctcgct tggcaacatc gcagctttac ctttctcgcc tgttggattt
     2341 tcgggagtga ttgctcgact ctggattttt ttaaattggg taatattttt tagtatactc
     2401 atctttttct cctttgaatg tgaatcgttt ttcccttttc tacgtgaaga atttttttcg
     2461 tatcacttat tttcaatggc ttttctgaca aatttgaaat actcaactcg gattgcgtac
     2521 attcaatcga aatgaaggct tgaccgaact tcaaattctt ccaagaaaac gttaaattat
     2581 caacaggatc aatttgcaat actccttttt tgaatttcaa tccgaaatat tgactgatca
     2641 ttaggctgac aaaaagaccc gatgcccagc cgcatttgcc gcagccaaag tctcgcacga
     2701 cttctccttg ttgagcctct aagcgatacg gccaccacca ccacgaaccg tctaaatctg
     2761 ccaaacaaat tagttcttct aatcgggtta cgaaagcttg agggttgtgc agctcgctca
     2821 ttaaaacagt gataaagcca ggaaaagtgg cgccggattc taatccccaa gtgatgcctt
     2881 tgatttcctc gctataggtt ggattgaaag aactagcaga aaaggccatg gtgttctgat
     2941 ataaatgatc tttattatct aaaaattgat aaaaaggcat caacgtagtg tctgattctt
     3001 ctccatcatg catcaataaa ttaatttttc catccacaat cacatcactt aaaaagatta
     3061 acccttgctc aagaatcggt ttttgatagt gcttcactga ataggttgtt ttctcctcat
     3121 caccgattcc ctccaaaaat tgctcaccaa aaggaccatc gatagtcatt tgtttacaaa
     3181 ttgattcctt tagtttacag gcaatgtttt tgtatcgctt gctaagactc agttgttcta
     3241 gggcttcaaa aatttctgca agtccaatac aggccttcca aagacaaaga ttgctgccgg
     3301 tatgatactt tccaagtgca aaggcgtctg ataaccaaac agatggaaaa agcattggtt
     3361 cccctgaccg acgttgagct aatatgtcct caataattct tgaagcattc agtagaacct
     3421 gcggacaccg ctcaaagaat tttgtatccc cagtatgttc aaaataaaga ctagccagta
     3481 acgcactgga taacgaatta ctcagagaat gattgatacc tcctgtaaat tttgaaccag
     3541 gaaacttaac gccatattga tcaaaccata gaaccgtttt ttgacataac tccggttcaa
     3601 acatggtgaa tggcaatgat gcgtaataca tatccttatt ccaaattcga ttagtagcag
     3661 gatagctgcc ccaattgctg ccgacgacct gtccctgtcg attcataccg aaactagaaa
     3721 agctttgata aagtgcacga cgatagagat ggacaactct atcgtttggc attgacagct
     3781 gcccaaatag ctgagcaaaa tagttcaatg tttccttcag ccatttttcg atatcagatt
     3841 cctgaaaagt cattacttct tcaaaagcat ttgggtccac aaaagcaatg gcgaagcttg
     3901 ccacttcctg ttgtgcgatc gtgtccttat ttcctttttg agaaatcaac acattttgct
     3961 gatccgaata cttctcttga tacgaaggca catctttcac tgaaacttga aggctttcgt
     4021 gtgtttcatt cacaatgtaa acctgttgaa caagcatact taaacgctta ttagccagta
     4081 tgggagcaaa ggatactaca atgaccttaa acgtcgaata atgatgaata tatctaggca
     4141 aacagtcttg aatcacatcg acttcaatac gatgatccga ttctgacaaa tggtagacaa
     4201 tcccgtttac ttcaaccttt aaggataacg aattggtttg agagagctgt ttattgaccc
     4261 aaacacctgg tttttcctca gttagtaaat tttctcgata aaaggtcaac gtttgaatcg
     4321 tcccgcaaac atcaaggttt gctaatgtct tttgattact tacatcgaag gtcaatccct
     4381 ttacatcagg aatattccga tagtagctgt attcgtttga agtagtcaca tttccgtcgt
     4441 ttaactcagc attaaaaaat gggtgtttgg gtgaataaat tacttcatta atagtcatct
     4501 tttccctcct tgctaaaaaa actgcagcct agtagctgca gcttcctact atgcgattcc
     4561 aagtaatgac aataaaattc ccaagacgac cattccaaaa ataattttga tgacgccgac
     4621 ttttttatct agtaatttga aacagctcaa tgttaccaat aacggtacaa ttcctttaaa
     4681 aatactatcg aggatttctt ggacattcat tagttcttcg cccttcattg aaaagctcaa
     4741 aacggtattg aatttgacca tactgatcgt cattgcacct accatgatca gacccaagat
     4801 actagcagct tttgtcagaa tttcaattaa cccgttcgca tacagctttt caatatattt
     4861 agagcctaat gaatagccta aaacgccgcc atagtatcgc atcaagtaat taggtatatt
     4921 gaacataact aaaaagataa ttggtgccaa aatatttccg gcattggcta agttgatcgc
     4981 tagtcccgcg gtaacgacac gccaaactcc ccagaacaat gaatcgccaa ttccagccaa
     5041 tggtcccatc aaactcgttt tgatcgcgtt gatactttcc acgtcaaaac cagctttttc
     5101 agaattttcc ttctccatcg atgccgcaat ccccatgaca aaggacgcgc aaggaatggt
     5161 cgtcgaaaaa tactgtgtat gacgttccag cgccgcagct cgatcttctt cacttttgta
     5221 gaaatgatta atgaagggca tcatagaata ggcgaaacca ccgccgccca tcgtcaccgg
     5281 attcaccgaa gcatacatag tcatcgagcg ccaaaacatc ttccgaacca attttttctc
     5341 atctttagaa attccatcag ctaatttcat tgaaagaaat cctcctcttc ttggtcaagc
     5401 gttgcattta cagcagcatc gctttgaact gcatgaacag atatattttt tagttgtaga
     5461 tctctttggg ctataacaat tgcaataatc gttccaactg cggcaattgc aatcaatggt
     5521 aactcgagat acgccataac aacggcccca aagaaaaacc acacagcaat atttttactc
     5581 caaagcatcc gtaacagcat cgccatccca actgccggca gcagatttcc agcgactgtt
     5641 aatccgttca tgatgacctc tggaatatta tccaagacgc ttttgacaac atctgctcca
     5701 aataaaacgg caaagaatgg aataagagca taaagcgcat agtggacaaa ccacataaag
     5761 aagtgcagat tgatgattcc tttttgatca ccagattttg caaatttgaa aaatttagca
     5821 gccatcggtc cacatacaaa agaaaaaatc atattcatta actgtaatcc taatactgct
     5881 agtggaaaga caatgggaat aatcgcatcc acgtttcctc ccattttgat tgcgaacgct
     5941 gtagccagcc cagcagccaa agaaggctcc gccgtactag agattccgat attgacgaca
     6001 cccataaaga tggcttctag tgacgccccg acaatcaacc ccgtctgcaa atcccccatc
     6061 aacgctcctg ctaacggagc aacaacaatc ggacgactca gcatggacat cccgaacatt
     6121 tcatgtccag caattccaat aaaaacggcc aaagcgacta caaatgcatc atacataatt
     6181 tttctccttc ctttttctaa aaatcactca aaatagagat agctgaacgt ttttccatcg
     6241 ccggtgtagg ctgaaaaacg gtatctggat aaagttcaac taattctttt aagtcttgaa
     6301 tttctgacgt cgttaacatc acttcctttg ataaggtttt cttatcttcc gatggcggtg
     6361 tcattttccc agcattcccc acgttcatta attcgatttc cgttgggaaa gctttcgcaa
     6421 cttttaccgc atctttgaca ttagggacta aaaccatcaa gcgcatgtcc ttcgcccgcg
     6481 gatcttttaa caaatgaatc gcgtcatcaa ctgttttaat aatgactttg acattcgatg
     6541 gtgcagccat cttcaatgtc atcttttgaa tatcgttggt agccgcctca tcgcttgcta
     6601 ctactaaccc ggttaaattt agactctttg tccaagtcat cgctacttgt ccatgaatta
     6661 atcgttcatc aattcgcact gctttaatca tttttcttcc tcctaaaaaa aatcttcctc
     6721 ttcatttact acatgtacct catcaagaat ggtcatcttt aattcctgtt ggcatttatt
     6781 taccagttcc tgaatatcct cctttgttaa ttccttggtt gccaatacta tttcgagcag
     6841 aattggcaga ttaaagccag acaaaatagt tacagcaatc tctttctcca aaaaggtttt
     6901 ggtaatcttc tgattgacac ttccgccaaa taaatcggta aaaacaaaaa tctgatcctg
     6961 ctcgttcacc tggtttacaa atacattcaa ctcctgttca aaatcacact catccacata
     7021 agcattaatc accgtaatct cttgaacctt ccccgtcagt aattgaatac tgctaagaaa
     7081 gcctgaagct aagtgaccgt gagtcgctat tagaattttc ttcatatggt taattccttt
     7141 ctttctgttc acttttatat aagcaattcc tgtgccaact tttaaaaaca ttggtttaat
     7201 cacgttaatt cacattaaaa aaagtgtcta acctaaaaaa aacagattag acactttttt
     7261 agacacttta tttttgaata tacggtccta ataattcata gagcagcgaa agctcataag
     7321 cattgacttt aaaattatat ttcatctcca ttgattgaaa aatacttcta gaaacataat
     7381 aaaattcatc ttcatctggt ttcaaatcat aattcggttc ttcagatttt tctgtccgag
     7441 ccaagaataa acgttccatc atcaatgccg tatgcatata gagatttagt tttacacggc
     7501 catttaaagt gaaatgatag taattttcat atttagaaat gactgtttcc acttcactca
     7561 tcacgatcgt tgggttcaga aagttcaaac ggtccgcaat tccttccaac gaaaagaatt
     7621 ttactagctc ctgaatcaag cggttaaatt ctgccggctt catttcagga ctcaaaatat
     7681 gctccaaatt ggctgctcca tcaccgtcta agatgtcata aacattaata ttaggaattg
     7741 aaaaggaacg tggaagatcc gaagtcgtga tgaccaattt tgtcttgtca aaataagttt
     7801 catcattttg gtcgattaat tcacgtagtt ctctgtagtc cttcgtgaaa acttcgatgg
     7861 attcatccga taaatgatga cgaaaaacat ctttcaattt gtccgaaatc cccaaacccg
     7921 acatacagga aatgataatg ttgctacttt gagaaaagcc ttcaaagtat tgaacattga
     7981 ttgaatactt gtctgctgca tcctccgcaa tctgtttaaa tggcgcattc atcgtcatct
     8041 tcaagccaat atcaagtgca atggaagtcg tcaaattatt cagaagaagt aactcccctt
     8101 ttaaatcgtt cttgatctgt gtgtaaattt gatttaaaga gcccatatct acgatcagca
     8161 ctagactatc tgtattcact tgccgtttca cgaaattcct tgtcgattcg acaatctctg
     8221 acacattact ctctactggc atatccaacg cttcaaaaac ataattgccg cataattgat
     8281 tcactactgc ttgaatgctg ctagccgtac tatctccgtg agcaagcaac aggcccttca
     8341 gctcgatgga ttcatctaca taatcagata aagtcacgac aataagcagt cttagaaagt
     8401 tctcgtcctt ttctgagagc ggtggtaaat gctccaagaa ctgattacat aagtaagtcg
     8461 tccgaggtag atttgcagct aatgtacgat ttatcccggt taagtctgtc ttatagcttg
     8521 gggaaatgac ataggatctg ctaataattt ctgcaacagg tccactcgtc gtcaatccgt
     8581 acgtttttcc taaaacggat acccagacct ttctaaacat ctgaatcatc aattttctac
     8641 tcattgataa aaaagaatcc ttatcaatgt gatgcaccaa atgctgtact tcattcttta
     8701 tcgaagaaaa attttcattg aaattacctt catttatcag ctgagtaatt ttttccaaaa
     8761 tcgaatcata atcaaacggt ttgattcgat ccgtttttat cttttgattt ttctttatca
     8821 ataaactagc aatcggcaag ctttctactt cggattccag catatttaac tgaatcaaga
     8881 tctcattttt cccaatcgtt aatggttctt cttgtgttcg actatacaca tccgcacaac
     8941 tgattttaat gatattcttc agcttaccaa tattcccact aaagggatga gataacatta
     9001 aagacaaggc ttctcttgaa acaataatat ctttttgtaa aacatccgct tcattttgaa
     9061 aaaataattg aatcagctct aatcgttctc gaatggggcg atcttcaaaa gttggcaaca
     9121 taacactaat ttgaatacgc cgatcaaagg tgtctaataa ataattttca cctttttccg
     9181 tcgtggcgaa aataaaacga accttagcag aatgccaatt tacattttca ccgactggac
     9241 gatagtttcc agtatcaata aatagaaaga gcttttcttg attttctttc gataggcgat
     9301 ggacctcatc gagaaaaaga tagccgttgt tggccgttgc caataaacca tcggtatcct
     9361 tttcagcccc cgtaaaggcc cccttcttgt atccaaacag tgttgccgaa agcagttcgg
     9421 gattgtccgc ataatcggca cagtttaaga tagcaaatgg tgcatctttt tcaataattt
     9481 gctcatggat agcgtattca aaaatttttg tggctaaaaa actttttcca acacccgtgg
     9541 ctccatttat taatacactg agtccgttag gcggatattt gacagcggct atacattttt
     9601 gaatgacctc tctcagactt ccgtcatgac caactacaga atgaaaggct tcgtttattt
     9661 gctccgtatt atctacaacc gcatattcat ctaaaggctt ccacaaaaca ggctttcccg
     9721 gtaatttctc gaccttttta tcttccacta atctcgtcaa ataatggctg gcattctgac
     9781 gagagatacc tagattcttc gaaattttct cggcagtgac agaagacata ttccctgtta
     9841 gtcgtatcag ctcatcaaaa actcgatctt tagcgctctt tttcattttt tcctccagtt
     9901 taatgtgtct aaaagcataa taaactatcg gctgcaatat gtctattcta tatttgtatt
     9961 ccaattttta ctttgaaaca caaatagtcg aatatttgcc tcagatatct gacaaaataa
    10021 tttat
//
LOCUS       DAZULY010000100         9978 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000100 DAZULY010000000
VERSION     DAZULY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.101"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..116
                     /locus_tag="ACG81A_001614"
     CDS             <1..116
                     /locus_tag="ACG81A_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS5014978.1"
                     /translation="NIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            189..305
                     /locus_tag="ACG81A_001615"
     CDS             189..305
                     /locus_tag="ACG81A_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFS5014979.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(360..2042)
                     /gene="recN"
                     /locus_tag="ACG81A_001616"
     CDS             complement(360..2042)
                     /gene="recN"
                     /locus_tag="ACG81A_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFS5014980.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(2060..2509)
                     /locus_tag="ACG81A_001617"
     CDS             complement(2060..2509)
                     /locus_tag="ACG81A_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFS5014981.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(2616..3434)
                     /locus_tag="ACG81A_001618"
     CDS             complement(2616..3434)
                     /locus_tag="ACG81A_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFS5014982.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(3443..4333)
                     /locus_tag="ACG81A_001619"
     CDS             complement(3443..4333)
                     /locus_tag="ACG81A_001619"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS5014983.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(4335..4559)
                     /locus_tag="ACG81A_001620"
     CDS             complement(4335..4559)
                     /locus_tag="ACG81A_001620"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFS5014984.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(4564..5901)
                     /gene="xseA"
                     /locus_tag="ACG81A_001621"
     CDS             complement(4564..5901)
                     /gene="xseA"
                     /locus_tag="ACG81A_001621"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFS5014985.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(5902..6756)
                     /locus_tag="ACG81A_001622"
     CDS             complement(5902..6756)
                     /locus_tag="ACG81A_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFS5014986.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(6853..7302)
                     /gene="nusB"
                     /locus_tag="ACG81A_001623"
     CDS             complement(6853..7302)
                     /gene="nusB"
                     /locus_tag="ACG81A_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFS5014987.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(7295..7720)
                     /locus_tag="ACG81A_001624"
     CDS             complement(7295..7720)
                     /locus_tag="ACG81A_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5014988.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(7966..9030)
                     /locus_tag="ACG81A_001625"
     CDS             complement(7966..9030)
                     /locus_tag="ACG81A_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFS5014989.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(9202..9447)
                     /locus_tag="ACG81A_001626"
     CDS             complement(9202..9447)
                     /locus_tag="ACG81A_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5014990.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(9586..9960)
                     /locus_tag="ACG81A_001627"
     CDS             complement(9586..9960)
                     /locus_tag="ACG81A_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFS5014991.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
BASE COUNT         2741 a         2247 c         1644 g         3346 t
ORIGIN      
        1 tgaacattaa gttacctatt gctgaaaggg atgatgcctt tatatggcta gttctattaa
       61 cattatttat ttgtatcgta actacctatt atttaaaaaa agggaagttt ttataaaaaa
      121 gccctcttgt ggaaatggaa aaaagattgt acacttgaat caactaatta atgacgaggt
      181 tgaaaacaat gcgtgaaaaa aaacaaacaa gtacttttgc taaagtaaca aaatttgtca
      241 tttggacaat gctgatttta acgattggat ctgttgtatt gactgctgta atgagtgtca
      301 tgtaaaaata aaatatagct atcagctgct gaaaaggacc tgaaccactg cgaggtggct
      361 caggtccttt ttgcgagttt caatagttct tttgcatgtt cgagtgtgag ttttgtaatc
      421 tcacttccag ccagcatacg tgcaatttct tcttctcttt cttttgtttt caactctgaa
      481 acagaagttt gcgtacgtcc atcaatcact gctttcacaa tataatattg atagtcggcg
      541 actgcagcaa cttgaggcag atgagtgatg cacaaaactt gagagtattt cgatatttga
      601 gaaattttat ctgcgatggc ctgtgcaact cttccgctca cacctgtatc cacttcatca
      661 aaaataatac ttgtcacacc ttgctcagaa gaaaaaatag ttttcaaagc aagcaacatc
      721 cgagacagtt cgccaccaga agcgactttc accaaaggct tcagtggctc tcctggattg
      781 gtcgtaatgt aaaattcgat ttggtcaaat ccatcatgat tcaattgtct attttcttct
      841 tttaagaaac gtacctcaaa ctcagtgttt tccatataca gacttttcaa ttcatgcaga
      901 atggattttt ccaattcttt tgcgattttt ttacgcgctt catgaagatc tgcggcttgt
      961 tgctgaagta gaagctcttt ttgctctagt tcctctttca ttttgtccaa ttgaccttct
     1021 gaaaaatcag aagaagctag ctcttcggtg atttcgtcat agtaagccaa tattgactca
     1081 atggattcgc cgtatttacg tttcaactgt cggattaatt ctaaacgttg cgtaacttct
     1141 tctaaacgac tctcatctaa ctccaataaa tcaatctgac gactcatatc gccgattgca
     1201 tcttgcagca aatagtaagc actttggata ttttcataaa ttgcttcata ctccacatct
     1261 agatgggcga tcccttgcag ttctgaaacg gccaagccaa caccatctac actgttttgt
     1321 tcattattcg tcagtgctcc gtaacctgca gctaatccat cagcaatttt ttgataattg
     1381 ctaagttttt ctctttcttc ttttaatttc tcttcttctc cgacttgcaa atcagcagca
     1441 gctatttctt cttgctggaa atggagcata tctattctct gaacatagga tttttcattt
     1501 tgctgcactt ttcgtaccct tttttccagc tcacgataag agatatattc ttgctgataa
     1561 ctctttttct tctgttggaa agctacatct ccaaaacgat ccaataaagc aagatgtgat
     1621 tcaggctgga gcagttcctg atgttcgttt tgtccttgga tatctactaa ataactgccg
     1681 attcttctca gattagctaa tgtgataata tgaccattca ctcgacaaac attttttcca
     1741 gttaaggaca tatcccgtcg aacaatcaga ttgtcctcat ctgtttcgat ccctaattca
     1801 accatcagtt cagagaatcc ttcttgtttt ggtagttcaa acaacccttc aagcacacat
     1861 ttttctgctc cttgacgtat ataatcgctt gaacctcttc ctcctgctaa cagacctaaa
     1921 gcatcgataa taatggattt acccgcacca gtctctccgg tcagcgcggt cattccttcg
     1981 tgaaaagaaa gccgcaattc cggaataatc gcaaagttag taatactgat ttcttgtagc
     2041 atcacattcg ctcctcaact taaaggtaat gcaggaagtc tttcttcaat aaaactgcct
     2101 ggtcttcagt tcgtgtaatc atcaaaacat tatcgtcatc attgataatc gaaaataatt
     2161 ctttcttgta acgtttatca atcaaatttg ctgctgccga agcatttccc ggcaatgttt
     2221 tcaaaataac aaatttttcc atttggtcaa ctgccacgaa agcgtctttt aatagttttt
     2281 ccaatttagt atttacatct tcgtttgttt cagctggtat gctataacgg tagccgccat
     2341 ctgaagccgg taccttgatc agtttcagtt cctttatatc tctggaaatc gtagcttgag
     2401 taacagatat tcctttgctt ttcaggattt caacaaattc ttcctgcttt cggatatctt
     2461 gctcactcaa caatcgagta atcaaacgat gccgatcttt ttttctcatt acgtcttcgc
     2521 tccttagcat attcttctta atcaatgata gcttatttcc ttggtataat aaagaagaat
     2581 ccgcatactt ctcacttgat tttcacagtt acaatttatt tatggtcaaa ctgttcgtgt
     2641 gcatttgaaa ccacttctgc catattaata gctgaattga ttgttccgct ttcgggtact
     2701 ttcctcaagt gagccaagaa ctcgatattt ccttctcccc ccgtgatcgg agaataactt
     2761 aattccagca catcatatcc accttgtgcc gcaaatgaca aaatgtcttc taaaactttt
     2821 tgatgggtct gagggtcacg aacaattccg tttttcccta cagcttctct gcctgcttcg
     2881 aattggggtt tgattaaagc aatcacttct ccctcttctt ttaaaatagc atgtaatgga
     2941 ggcagcatca gttttagtga aataaaagaa acatcgataa cagcaacttc tggaattcct
     3001 aaggtaaagt cttcgggttt tgcataacga aaattcactc tttccattac gacgactcta
     3061 ggatcttgtc ttattttcca agctagctga ttgtagccaa cgtctaaagc ataactcatt
     3121 tttgcgccat tttgcaaagc tgcatcagta aatccgccag tggatgcacc aatatctaac
     3181 aatgtttttc cttcaacaga tagatcaaat gctttgagtg ctttttctaa ttttaatccc
     3241 cctcgagaaa cataaggaag ttttttacct ttgactctta attcactact cactggaatt
     3301 ttttctcccg gtttatcaaa ccgctcgttt ttttcgttgt aaatcaaacc agccatcact
     3361 gaacgtttgg cttgttctct tgtttcaaat aatccttgtt tgaccgctaa tacatctacg
     3421 cgttcttttt ccattctttc cctcatttct ccacatcaaa ttgcttgatc atctggcgca
     3481 tcagcccgcc atcaaaagaa aaaacgtcct cttctaattt gtctatgatc ttgttggcgg
     3541 ataacagctc aatctccaac gcctcacgtg tcttttctag gcctaataaa cgcgggtaag
     3601 tatttttttc catacgttca tctttaccag ctgttttccc taatgttttc gttgtaccaa
     3661 tcacatcgag tagatcatct cttatctgaa aagctaaacc taaatgttcg gctaatcgtt
     3721 gaagtagcaa taagatttct tcaggttgct tcgctaatat gccaccagcc agcaaggcat
     3781 aacgaatcaa atgccctgtc tttctttcat gaataaatgc taattcttct aaagaaagct
     3841 ttttcgattc gccctcgata tctgcagctt gcccagcaac cataccttgt gatcccgcac
     3901 aaacagctaa ttgctgaagc agcagaagct taggagaatt acctaagtgt gccatactga
     3961 ttagctggaa tgctcctgta agcaaaccat ctccggccaa tatagctaat gcttcgccga
     4021 atactttatg attggtaggt tttcctctac gaagatcatc attgtccatc gcaggcaaat
     4081 catcgtggat caaggaatac gtatgcacca tttctaaagc agctgctact tgatagacgg
     4141 gaacatcaat aggctcatca aaggaagcaa cggccgttaa aagtaataag ggacgtattc
     4201 ttttcccgcc tgctttcact gaataactca tcgcatcata gagtcctcta tccgttgtat
     4261 gctcatctaa aaaagacaag atttcttttt ctacagcagg tagatgaata gctgaaaagt
     4321 cagttaatct catctcagct ttcctcgttt ccgtcaaaat caacttcttc atttgattct
     4381 gtcatcattt tcgtcaaggt ctcttctgct ttagctaatg tatcttggca atgtttgctt
     4441 aattccattc ctcgtttaaa cgaatctaga gctgattcta atggaacgtc tccttgttct
     4501 aactgcatga caattttttc taattcttgc agtgactctt caaaagttgg attttccata
     4561 tttttattct cctttgtcta tctcttgtac ttgtccttta acgattccat cttgataatg
     4621 gatcgtcagc aaatcccctt gatgaatctc ggtgacactt tttactactt gatcatttaa
     4681 cgtcgtatag ctgtagcctc ttcccatgat tttcaatgga ctcaacaaat caagagattg
     4741 aacagcttga gcaaaacgct gctgctgttg tttcatatat tgttggattt tctcttctaa
     4801 gcgtttttct agataagcag tttcttggcg agctgcttta actcgataaa gcggagccac
     4861 ttgttccaat tggtggctta atgcgatcgt ttgtttttct ttcgcatgat aaatttgctg
     4921 tgtagcttga tataatcgct gtttcaactg gtctaatttg atggtctgac cttcatacaa
     4981 tctttcaggc tgccgaaata cataggaacg tcgagcacgc tcgaaccgtt cttgtttgcg
     5041 ctggatctgg cgtacaaacg cctgctgcaa tctagcttgc cgttcttgaa tgcgcatcaa
     5101 ctcttctgac aaaacaggaa cagctaattc tgcagctgct gttggagtgg cagcccgaac
     5161 atctgctact aaatcagcga tcgtggtatc tgtttcatgt cctacagatg aaatcaccgg
     5221 cgtattagct tgataaatcg cccgagcaac ccgctcttca ttaaacggcc ataaatcctc
     5281 aatcgaacca cccccacgac cgatgatcat tgtatcgaaa ttgcccatct cttctactct
     5341 ctggatattc ctgacaacat tgtccgctgc ttggtttcct tgtacaactg ttgggaacaa
     5401 aaccaattgg acaattggat atcttctttt caccgtcgtg atgatatcgc ggataaccgc
     5461 tccgcttgga ctggtaagga ccgcgattcg ctttgggaaa cgtggcagca tttttttagg
     5521 cccttgaaaa aggccttcct tcatcagctt ttcttttcgt tcttccaatt cttgatataa
     5581 agcacctatt ccatcaggct ccatgtgctc tacatagatt tgataagaac ctcccgcttc
     5641 atataatgaa atacgcccaa cgacaaggac tttcattcct tcttttggct ggaatcgtaa
     5701 tttttggaat gctcctttga acataatagc agatattttt gcatggtcat cttttaagct
     5761 gaaatattga tgggcattgg cacgtaaacg gaaattcgat atttcgcctg ttaaatatac
     5821 gcgttctaaa taaggatcgg catcaaattt tcttttcaaa tacttcgtta atgtagtgac
     5881 agttaaatat tcttgtgcca tctattcacc tgctccaaaa gcttcggcag attttacggt
     5941 ttgatacatc agcatcgtga tagtcattgg ccctacaccc tttggcacag gagtgatcca
     6001 gcctgcaata ggctccactt catcaaattt cacatcaccg atcagcttac cgttagcatc
     6061 acgattcatc cctacatcta tcactacagc acctggtttt acgaattctt ttgtgacaaa
     6121 atgtccacgt cctattgcta cgactaaaat atctgcttct tgtgccagtt cagccagatt
     6181 ttcggtttta gaatgagcaa cagtaacagt tgcatctgcc atcaagagta gctgagccat
     6241 cggtttaccg acgatattgc ttcgtccgat aactactgct cgtttacccg ccggatcgat
     6301 agaataagct tcaaacattt tcatgatgcc gtatggagta caaggaatct tgtccggttt
     6361 acccgcaagg agtctgccta aattcatcgg atgaaaaccg tctacgtctt tttcagggcg
     6421 gatagctaac aggatctttt cttcatctat atgctttggc aatggtaatt ggaccaatat
     6481 accatgaaaa ctaggatctt gattgtattt ttcaatttct gcaagtagtt cgtcttcaga
     6541 aatcgtctca ggataacgct ctaccaatga atggattccg atttcttttg ctacacgttc
     6601 tttatttctt acatagattt ggctggctgg attttcccct accaataaaa cgactaatcc
     6661 gggatggata ccattatctt tcaatttttg gattttttca gcaatctctg cctgcatttt
     6721 ttcagctaat tcttttccgt taatcaattc tgccatctaa ccactcccag atattttttg
     6781 tgttttcatt ctaccataga attttcccga tttataacag gtttatgatg attcatagaa
     6841 aaaagctagg acttacgccc cagcttctaa ttctttcaat acattggata aaacaccgtt
     6901 gacgaattta cgtgaacggt catcactaaa tgtttttgcc aactcgattg cttcatttaa
     6961 agcaacagcc gctggtacat ctgtcacata agtcatttca aaaatcgcga tacgcaagat
     7021 caccaaatcc atttttgaga tccgattgat gtgccaattg ttttttaaat gtttttcaat
     7081 cactttatcc aattcatctt tttttgcaca gacaccgcca actagcgtat caagataaac
     7141 aggaacaaac tctgattcat cttcgttgat catgtcgcgg tggtctaatt cgattgcgtt
     7201 gaaaatcgca tcttcttttg ttaggtctgc attgaagtct aatggaaata aagcctgcaa
     7261 tgccatttcc cgaatctcgt gtcttgataa ttctttattc attctcttcc tcttcgtctt
     7321 caaaaagttc ttcgattgtt ggttgttcta atttttcagg aacaacagct actacatgga
     7381 tattgacttc agataaatcg atatccgtca tgaaaaggac ttgctggctg acccgttcct
     7441 gcatttccat tgctacttta ggaactgaaa caccatattc tagataacag taaagatcta
     7501 ctttcaagcc atcttcctcg tttgctaagt aaacaccttt tccgtgagct gcacgtccta
     7561 ataattcagt gacattgctg gcaaaagttc cgcgcattcc gtatacgcct tctactttag
     7621 aagctgcaat cccgataatt acttcgatga cttcaggagc gatcacgatc tctcctaaat
     7681 cttggtttgc gtttaaaatc aaattttttt catctgccat ttatttattc ctccctttgc
     7741 ctaagcacga gatacatccg aattctctat gatgcggcat agtgatcacg tggtaagaaa
     7801 actgccgccg atcagctgca aactgctatg aattggagaa aacacccaac tttattatct
     7861 cttacagctt aaaagttctt ctatagtgta tcattaaatc gagtgatttt ctataacaaa
     7921 gtctgtgaac actgattttt acgcttactt cttctattga tgaaactata agatgatcaa
     7981 ttcttttggt gaacgagtca acacacgatt tccatcttct gtgatcaaca agtcatcctc
     8041 aatacgtact ccaccgattc ctggtaagta gatccctggt tcatcagtga tcacatttcc
     8101 tggtacaaat tgcttttctg cacgagaaga aacgtttggt ccttcgtgga tctctaaacc
     8161 gatcccatgt cctgtagaat gtccaaatgc ttccccgtaa ccatgttttg aaatataatc
     8221 tctagcaact gcatctaatt ggacaccagt cattcctggt tttgcagcat caataactgc
     8281 taattgagca tctaagacta tttgataaat ttcttttaat ttttccccag gatcaccaat
     8341 agcgaatgta cgtgtcatat cggaaacgta cccttcgtaa tagcaaccaa agtccaatgt
     8401 gatcaagtca ccttgttcga tgattttttt gcttgctaca ccatgcggca tagccgaacg
     8461 caatccgcta gcaacgatcg tatcaaaaga aacacctgaa gcgccaagag aacgcatata
     8521 aaagtctagt tgatttgcga cttcaatctc agtcatgccc ggttggatca ttttcaggat
     8581 gtgatcatat gccatatccg caatatgaca tgctttttcg ataatagcga tttcttcttc
     8641 atctttcact tctctgagtt cttcgatcat ctgtgaaaca ggtactaatt ccgcatcgat
     8701 gatttcttct aaaaccgaat attctaaaaa tgagactgtt gtttcctcaa aagctaatga
     8761 gtgcagttct tctttgcgta ctaagtttgc tacctcatca aaaataggtc cgacattttt
     8821 aataatttca aacccttgag cctgcgctgc cgcctgctcg gtataacgaa aatcagtaat
     8881 aaaaaatgct ttttccaaag taataacagc taatcctgtc gtacctgtaa agttcgttaa
     8941 gtaacgaaga ttgtacggac tggtaaccaa aaatgaatcc aaattttctt cttgcatttt
     9001 tttgcgcaat ttttctacac gtagcatcat aatgaaactc cctctttctt tgcacgcttt
     9061 tttccattat agcaaacttt tcttcgtaca cgacagttaa ataaaaattt ccggaaaact
     9121 tctttttatt ttaaataaat aaagccaaaa aggagtcaca gtctcgctgc aactcctttt
     9181 agtttactat tttgtaaaaa cttatgatcg ttttccaaag aaaaatgaga caactgctac
     9241 caaaatcact gcgcctaaaa tagatggaat aagtgccata cctgcagctt ttggtcccca
     9301 ttcgccgaaa atcgcttgtc caatacttga accgactagt ccagcaatga tattcgttac
     9361 acagcccatc gaatttcctt tactggtgat cgcacctgca attgctccga tcacggctcc
     9421 aacaatcaat gtccataacc aactcataat ataacctcct tctttgctaa tcaattttag
     9481 catatgggat ttaaagggga caaataattg gttttgctat ttcatatttt cttctgcaaa
     9541 gtaaaaaaat aaagaaaacc tcctcttaga agttttcttt attttttact tgcgtttaaa
     9601 catactacga ataccacgtt tcactacatc aaagaaactc aatgattgta ccattctatc
     9661 ttcatctaca tattctctga tcgcacgaag cactttttta ggttctttgg aagaaaatgt
     9721 gtatgtacca ttttttttcg tttgaatggc atatcgagga atccattttc cctttagcat
     9781 gactgatgct agtacataat ccacttcttc ccatggaatt tggatatatt tgcgtctatc
     9841 tcgcgcatgg taaaactcaa aaccattatc acctatcatg atttgcccgt aatctgtcag
     9901 acccgtaaac gaagtagcat caattaccaa gtctactttt gtattgattg attgaaccat
     9961 tttctccctc cagatatt
//
LOCUS       DAZULY010000101         9959 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000101 DAZULY010000000
VERSION     DAZULY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.102"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..699
                     /locus_tag="ACG81A_001628"
     CDS             <1..699
                     /locus_tag="ACG81A_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFS5014992.1"
                     /translation="NYYEGGNEIVSKMKISEVTLDETDDFTYYKGYKKGWNTLCTYLK
                     IRVPFDDLDFFESHKDLITQTASSIYDKQGDNVLVDTILVPLPENYEVINFSQLKISD
                     VVSQAIEDAESFMSNGEYQRAFDRVHTAFHGYLIEILKKYEITVPRDENLSKLYSRIQ
                     QLIEKEIQPTELADIVKTTIRSSNGMISSLNEARNRHSLAHPNTNIIGKREAKLIIGI
                     SSTVTDYISGYLDK"
     gene            760..1260
                     /locus_tag="ACG81A_001629"
     CDS             760..1260
                     /locus_tag="ACG81A_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS5014993.1"
                     /translation="MEMKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEE
                     YAIHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENK
                     EDIHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHG
                     VFEYCQ"
     gene            1316..1954
                     /locus_tag="ACG81A_001630"
     CDS             1316..1954
                     /locus_tag="ACG81A_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6037 family protein"
                     /protein_id="HFS5014994.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            2026..2421
                     /locus_tag="ACG81A_001631"
     CDS             2026..2421
                     /locus_tag="ACG81A_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5014995.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            2405..4858
                     /locus_tag="ACG81A_001632"
     CDS             2405..4858
                     /locus_tag="ACG81A_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5014996.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            4855..6882
                     /locus_tag="ACG81A_001633"
     CDS             4855..6882
                     /locus_tag="ACG81A_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS5014997.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            6879..7901
                     /locus_tag="ACG81A_001634"
     CDS             6879..7901
                     /locus_tag="ACG81A_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFS5014998.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            7918..8847
                     /locus_tag="ACG81A_001635"
     CDS             7918..8847
                     /locus_tag="ACG81A_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5014999.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            9161..9208
                     /locus_tag="ACG81A_001636"
                     /pseudo
     CDS             9161..9208
                     /locus_tag="ACG81A_001636"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9224..>9959
                     /locus_tag="ACG81A_001637"
     CDS             9224..>9959
                     /locus_tag="ACG81A_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS5015000.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRG"
BASE COUNT         3399 a         1644 c         1984 g         2932 t
ORIGIN      
        1 aattattatg aaggaggtaa cgagattgtt tctaaaatga aaatatcaga ggtaacttta
       61 gatgagactg acgattttac ttattataaa ggatataaaa agggctggaa tacgctttgc
      121 acatatctta aaataagagt tccttttgat gatttagact tttttgaaag tcataaagat
      181 ttgattaccc agacagcaag ctccatatat gataaacaag gtgataatgt attagtggat
      241 actattttag taccacttcc agagaattac gaagtaatta actttagtca gttgaaaatt
      301 agtgatgtgg tttcacaagc tattgaagac gctgagtctt ttatgagtaa tggtgaatat
      361 caacgtgcct ttgacagagt tcacactgct tttcatggat atttaattga aatactaaaa
      421 aaatatgaaa ttacagttcc tagagacgaa aatctatcta aattatatag taggatacaa
      481 caattaattg aaaaagaaat tcaacctact gaacttgctg atatagtgaa gacaacgata
      541 aggtcatcaa atggaatgat tagctctctt aatgaagcaa gaaataggca ttcacttgca
      601 caccctaata caaatattat tggaaagaga gaagcaaaac taataatagg tatttcaagt
      661 acagttacag attatattag cggatacctt gataagtagc gtcattaact gattgctact
      721 ttttttatga ctatttttat aaaaaggatg tgataagaat tggaaatgaa agtctatgtg
      781 gcaaatttag gacggtataa tgaaggtgaa ttagtcggag catggtttac tccaccaatt
      841 gatgaggaag aaatggcaga aagaatcggt ttaaatgagg actacgaaga atatgcaata
      901 catgattttg agcttccttt tgatgtggac gaatacacgc cgattagtga aatcaaccgt
      961 ctatgtgaag ccattcaaga aatcgaaggt acacctattt ataatgaact caaagaaatt
     1021 caaggcatgt ggtttagtag cttggaagaa ttactggaaa acaaagagga tattcattgt
     1081 tatagtgatt gtgattcgat ggaagatgtc gcccgttatt acgtggaaga aacaggacag
     1141 cttggtgaag tcccttccaa tttacaaaat tatattgact accaatcttt gggacgtgat
     1201 atggaaatag agggtaatta cctagttacc tctcatgggg tatttgaata ttgtcagtaa
     1261 gtttaacaat ccgtgttata attaaattaa taaatagcac ggagaggtat aaattatggt
     1321 aaatgtattt gaaaatctta aactattaaa actagacatg aaagataaag gctgggtgat
     1381 agattccttt tactttcgtt acaaacagca aaattatatt gtacttgtta agctatttga
     1441 aaaagaggaa aaagttccag aatatgctct gttaaaacta gaatttttaa aggaaaacga
     1501 tttttcagat atgctagctg tctatgctaa ttcggtaaaa ttatatacag acacgaaaac
     1561 aataagagaa tattttggta tcgaatacag tagtaacttg ggagatgttt tatttcaatt
     1621 tagccaaact cttgctcgat ttattccgac agaagttagt gagaagaaaa atgaagacca
     1681 aaaagaagcc atgtgtttct cattaagtca aagtgattca gaagatccca gaaaaaaata
     1741 ttgtttttca gttagaagaa atcctttaaa gggaaatggt gagctgggaa aacgaagtcc
     1801 ttttaatgac aataaaacga gattgtatag acccagctta tatgaacgat tagggtcaga
     1861 taccaattta agtttcagat attcaatgaa tccagatgat gaagaaactg atgaaggtat
     1921 tattgcaaaa tggacaaaaa ataaaattga atagttaaag cattgaaaga gcaatcacct
     1981 attttgattg ttctttttct atttttacgg aaaggacgtg aagacatgaa aaaaataaga
     2041 agttacacca gtatttggtc agtagaaaaa gtgatttatg cgattaatga tttacaactc
     2101 cctttcccaa ttacctttac gcaaatgaca tggtttatcg tttctttgtt tgtcgtcata
     2161 ctctttggca gtttaccacc actttctctg attgacggag cactactcaa atacttaggg
     2221 attcccattg ttctcactgg gttcatgtgc caaaagacct ttgacggaaa gaaaccctat
     2281 ggatttctaa agtctatcat tacctatgct ctacgcccta aagtaaccta cgctggcaaa
     2341 caaatacgat taaaaaaaga aacaatgaat gaatcgatta cgattgtaag gagtgaacaa
     2401 ctagatggca tatccgatta aatatatgga agataatctc gtatttaatc atgatggcga
     2461 agtattcgct tattatgaat taattcccta taattacagt tttttaagtt ccgatgaaaa
     2521 aattcaagtt catgataatt tctgtcaatt gattgcccaa aatcgtgatg gcaagattca
     2581 tgctctgcaa ctagcaaccg aatcaagtat tcgagccaca caggaacaat cgaaaaaaga
     2641 aatcactgga cgattaaaag agatagctta cgaaaaaata gatgaacaga ccgaagcgtt
     2701 aatttctatg attggtgaca atcaagtaga ctatcgcttt tttattggtt ttaaactcta
     2761 cctcaatgaa caggaagtat ccgtaaaaaa tgttgggaaa gaaattcaaa aagcatttgc
     2821 ggaattcttt catggtgtga atcacaaatt aatgggcgat tttgtttcta tgcctatggc
     2881 ggaaatcgaa cgcttttcta agatggaaca gttgttagaa agtaaattat ccagacgctt
     2941 tcagattcgt aaattggaga aagatgattt tggttatatt attgaacatc tttacgggca
     3001 aacaggtgtc ccttttgatg actacgacta ccatttaccg aaacgatacg aagataaaaa
     3061 agtgttggta aagaaatacg atattctcaa acccactcgc tgtttaatcg aagaaaatca
     3121 acgctattta cggattgaac aggaagatac cactacttat gtggcgtatt ttacaattaa
     3181 tagtattgtt ggtgaattgg atttccctaa tagtgaaatt ttttattacc agcaacagca
     3241 gtttactttc cccgttgata ccagtatgaa tgttgaaatt gtcgccaata aaaaagcctt
     3301 atctaccgtt cgcaataaaa agaaagaact gaaagattta gatagccacg catgggaaag
     3361 cgacaacgaa accagtacta atgtggtgga cgctttagaa tctgtcgatg aattagaaag
     3421 taatttagac caaaccaaag aatctatgta caaactaagc tatgtggtac gggtatcagc
     3481 aaagaatttg gacgagctga aacaacgttg tgatgaagta aaagattttt atgatgatct
     3541 gtctatcaaa ttagtacgac cttttggcga tatgctaggc ttgcataatg aatttattcc
     3601 ctcaagcaag cgatatatca atgactatat tcaatatgtc acgtcggatt ttttggctgg
     3661 tcttggtttc ggtgctacac aaatgattgg tgaaaattct ggtatttacg tcggttacaa
     3721 tctcgataca ggcagaaata tttatctcca acctagcctt gcaagtcaag gaattaaagg
     3781 ctctgtaaca aatgcattag cgtccgcttt tattggttca ttaggtggcg gaaaatcatt
     3841 ctctaacaat atgctggtct attattcagt actctttggc ggacaggcaa ccattgttga
     3901 cccaaaagct gaacgtggtg aatggaaaga aaccttacct gatattgccc atgaaatcaa
     3961 tattgttaat ctcacgagtg agaacgacaa taaagggtta cttgaccctt atgtgatttt
     4021 aaaactcccg aaagattcag aaagccttgc cattgatatt ctcacgttct taacaggtat
     4081 ttccagccga gatagcgaga agtttcctgt cctgcgtaaa gctattcgga gtgtaactca
     4141 aagcaaacaa cgagggttgt tattggtgat tgatgaattg cgaaaagaag atacgcccat
     4201 cagcaatagt attgctgacc atatcgaaag ctttgtcgat tatgattttg cccatctgtt
     4261 attcagtgat ggcagtgtca cccaatccat tagcttggaa aaacaactca atattattca
     4321 agtggcagat ttggtcttac ccgattccaa tagctctttt gaagaataca ctaccatgga
     4381 attattaagt gtggcaatgc tgattgtcat tagtaccttt gctttagact ttatccactc
     4441 tgaccgttcc atttttaaaa ttgttgacct tgatgaggct tggagttttt tacaagtggc
     4501 acaaggaaaa gctctatcta tgcgattggt tcgagcagga cgtgccatga atgcaggggt
     4561 ctatttcgtt actcaaaacg cggacgattt actagatgaa aagatgaaga ataatattgg
     4621 cttgaaattt gcctttcgtt ccactgacct agtggaaatc aaaaagacct tagaattttt
     4681 tggtgtggac aaggaagatg aaaacaatca gaaacgctta cgtgaattag aaaatggaca
     4741 atgtttagtt tcagacatct atggtcgtgt aggggttatg cagtttcacc cgatttttga
     4801 agagttgctt catgcctttg ataccagacc gcctgtgcga aatgaggtgg aagaatgaca
     4861 caaaaacaaa agaaatttct tcactatagt tggataacgc tcctttgtgt gggcgttatt
     4921 ttacttttat taggcacgct gggcaatgca gtacaagcga caggcttagt tgatgaaacc
     4981 attgatacaa gcaatgaata ctccaagtat ggtttgaatc aatatcagct tgattattat
     5041 gtggacaata gctggggctg gcttccatgg aattggagtg atggcatagg acaatcggtg
     5101 atgtatggat tgtatgccat tactaatttt atttggacaa tttctcttta tctatcgaac
     5161 gccacaggtt atttggtgaa agaagcctat gccttagatt tcattagtca aacatcccaa
     5221 gcaattggcg aaaacatgca gacgattgca ggcattacac cgaatggctt ttccagttca
     5281 gggttttatg tcggtttcct ccttcttttt attctcattg ttggaatcta tgttgcctat
     5341 acaggattgg ttaagcgaga aacgacgaaa gtgatacgag ctgttttaaa ctttgtcgtg
     5401 gtgtttattc tatcggcttc ctttattgcg tatgcacccg attatattgc gaaaatcaat
     5461 gatttttcca aagatgtcag taccgccagt ctgaatattg ggacaaaaat tgttcttcct
     5521 aacacggata gtcaagggaa agacagtgtg gacatgatac gtgacagctt attctctgtt
     5581 caagtaaagc aaccatggct attgcttcaa tatggctcca ctgatgtaga agcgttaggg
     5641 gaagaacgtg tggaaagttt attgtctacc agtccagata caaacaatgg tgaagataga
     5701 gaaaatgtag tgattgaaga aattgaagat catgacaata cctatttaac cctaccgaag
     5761 acgatttcca gattaggaac agtcttcttt ttgtttgtat tcaatattgg tatctcgatt
     5821 tttgttttcc ttctaacagg aatcatgatt ttttcgcaag tgctatttat tattttcgct
     5881 atgtttttgc caatcagttt cctattaagt atgttgccga gttttgatgg catgggcagg
     5941 catgccatta ccaagttatt taatgtgatt atgacaaggg ctggtattac cttgattatt
     6001 accgttgcct ttagtatttc cactatgctt tattcgctgt cagcaagttc tccctttttc
     6061 atgattatgt ttttacaaat tgtgaccttt gcagggattt attttaaact gggcgattta
     6121 atgagcctat tcactttaca aagcaacgac tctcaaaaca tgggcagaca agtgtttcgc
     6181 agacctcgtc aaatgatgaa tcgacaaacc agaagatttc aacgcagtat gagtaagatt
     6241 tttcgtggaa aagcagggtc aacgaagcaa aagacaatga caacaaagca gaatcaaacc
     6301 aataaagcta accatactcg tcaaaatgaa cggactacac caaagaaaga aacagcactt
     6361 tccaatgtga aaaagcaaca aggcggtatc gggcaacgat taggtgaaaa gacagcccat
     6421 gtattggaca cgaaaaatcg tctagtcaat aaaagaaagc aggcaaaaca acagatacag
     6481 gcgacaccaa cccatgtcaa atatgccctt catcaaggaa aagagaatgt cactcataat
     6541 gtaacagact ttaaacaatc tatggcagat accaaagctc aacgcaaaca acagcgtatg
     6601 aaccagcagg agcaaaaacg aaagaacatt gctcaaaagc gtttggagct ggataaagca
     6661 aaagaaaaaa gacgaaaaga aaacccatca tctcctgttc agaataacca gcagcatgtt
     6721 cgcaagcgtc agcaaaccgt cacgcctgtt ttgtcacaaa acactaaacg tgaaaatcat
     6781 cagcgaccat tgaaaaaaga gcaaaaacaa aagacaccac cgaagcaatt caatcagagg
     6841 aagctaaata aaagaacgga taagcgagga caacaaacat gaagctaaaa ataacagtga
     6901 tttgtagtgt ggtcttctct ctcttctttt ttctcttatt attcctagtc attttctttg
     6961 cagatgatac ggacagtggc gaaaacaaca aggattcttc tattccacaa ggcggtgtga
     7021 ccgtttcacc agaagtgttg gcacaccgtc cgttgattga aaaatatggt aaggaatatg
     7081 ggattgaaga ctatgtttct tatatcttag ccattatgca agtggaatcg ggcggtacag
     7141 cagaagatgt tatgcaaagt tccgaatctt tgggtttacc gcccaacagt ttgagtaccg
     7201 aagagtccat caaacaaggg gttaaatatt tcagtgaatt attaaccagt gcagaacaac
     7261 aaggagtgga tatagattct gttatccaat cttataatta tggcggtggc tttttaaatt
     7321 atgtgaggag tcatgggaaa aaatatacct atgaattagc cgaacagttt tctaaagaga
     7381 agtcgggtgg tcaaaaagca gactatccca atcctatagc catacctgtg aatggtggtt
     7441 ggcgatataa ctatggaaat cagttttatg ttcagttggt ttcacaatac ctaaccgaca
     7501 cttccccaac agaatttgat gatgaaaccg tgcaagtcat tatggacgaa gccttgaaat
     7561 acgaagggtt tccctatgtc tttggagggg cgtcgcctac cacctctttt gattgtagtg
     7621 gcttaataca atgggtgtat gacaaagctg gcatttctct ccctcgtgtc gcccaagatc
     7681 agtatgacgc tacgcaagaa atctctatgg aagaagccca agcaggcgac cttatcttct
     7741 ttcattcaac ctataatgca ggcacatatg tgactcatgt ggcgatatat ttagagggaa
     7801 atcgctttta tcatgcaggc gacccaatcg gttatggcga cttaagcagt cgttattggc
     7861 aagaccatct gattggggct agacgtgtta ttcataacta agaaaggaat gaatcaaatg
     7921 attataaaaa tcgagcgaaa agaaaagaag gagaaaccaa agaaatctaa aaaaccgagc
     7981 aagcaaagaa aagttccgat gattaaagtt ggcacgcata agaaattaac ctttgtttta
     8041 tgggttttat tgattggtag tgtcggattt ggaatctata aaaattttac tgccattgac
     8101 actcatactg tccgtgaaac agaaatcatt aaacagcaga ttgtcgatac caaccaagta
     8161 gaaagttttg tgaaatcctt tgcgaaagac tacttttcgt ggcaacaatc ccaagaagcc
     8221 attgataaac gaaatgaaaa attgacccat tatttgacgg aagaattgca ggtattaaat
     8281 gaagaaatga ttcgtaagga tattcctact agttctagtg tgaacgatat acaggtgtgg
     8341 caggtttctc aagtgaatga gaacaccttt gaggtactgt tttctgttga acaagttatt
     8401 accgaagaca aagacaagga aactatatcg tctagctttc atgtggttgt ccatatagat
     8461 gaatcagata atatggttat tatcaaaaat ccgacaatga gtaagaaacc tcaaaaatcg
     8521 gactaccaac ccaaacagct agaaagtgac catacagtag atacagaaac gatggacgaa
     8581 atcactagct ttttagaaac gttcttccag ctctatccta ccgccacaga aaaagaattg
     8641 acttactatg taagtaatca tgtgttacct atgattaaca aggaatatgt tttcgaggaa
     8701 ttggtgaatc ctatctttac cagaaaagat aaccaagtaa tcgtgaatgt cgctgtgaaa
     8761 tatttagatc aagaaacaaa ggcaacccaa atctcgcaat ttgagttgat attagaaaaa
     8821 caagataatt ggaagattgt aaaataacaa atattggtac atgattacag atactttgta
     8881 atcatgtact ctttttgata aaaaattgga gattccttta caaatatgct cttacgtgct
     8941 attatttaag tgactattta aaaggagtta ataaatatgc ggcaaggtat tcttaaataa
     9001 actgtcaatt tgatagcggg aacaaataat tagatgtcct tttttaggag ggcttagttt
     9061 tttgtaccca gtttaagaat acctttatca tgtgattcta aagtatccag agaatatctg
     9121 tatgctttgt atacctatgg ttatgcataa aaatcccagt gataaaagta tttatcactg
     9181 ggatttttat gcccttttgg gtttttgaat ggaggaaaat cacatgaaaa ttattaatat
     9241 tggagtttta gctcatgttg atgcaggaaa aactacctta acagaaagct tattatataa
     9301 cagtggagcg attacagaat taggaagcgt ggacaaaggt acaacgagga cggataatac
     9361 gcttttagaa cgtcagagag gaattacaat tcagacagga ataacctctt ttcagtggga
     9421 aaatacgaag gtgaacatca tagacacgcc aggacatatg gatttcttag cagaagtata
     9481 tcgttcatta tcagttttag atggggcaat tctactgatt tctgcaaaag atggcgtaca
     9541 agcacaaact cgtatattat ttcatgcact taggaaaatg gggattccca caatcttttt
     9601 tatcaataag attgaccaaa atggaattga tttatcaacg gtttatcagg atattaaaga
     9661 gaaactttct gccgaaattg taatcaaaca gaaggtagaa ctgtatccta atatgtgtgt
     9721 gacgaacttt accgaatctg aacaatggga tacggtaata gagggaaacg atgacctttt
     9781 agagaaatat atgtccggta aatcattaga agcattggaa ctcgaacaag aggaaagcat
     9841 aagatttcag aattgttctc tgttccctct ttatcatgga agtgcaaaaa gtaatatagg
     9901 gattgataac cttatagaag ttattactaa taaattttat tcatcaacac atcgaggtc
//
LOCUS       DAZULY010000102         9947 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000102 DAZULY010000000
VERSION     DAZULY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.103"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(167..2287)
                     /locus_tag="ACG81A_001638"
     CDS             complement(167..2287)
                     /locus_tag="ACG81A_001638"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5015001.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2517..3695
                     /locus_tag="ACG81A_001639"
     CDS             2517..3695
                     /locus_tag="ACG81A_001639"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFS5015002.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3711..4469
                     /gene="aroD"
                     /locus_tag="ACG81A_001640"
     CDS             3711..4469
                     /gene="aroD"
                     /locus_tag="ACG81A_001640"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFS5015003.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4492..4977
                     /gene="ybaK"
                     /locus_tag="ACG81A_001641"
     CDS             4492..4977
                     /gene="ybaK"
                     /locus_tag="ACG81A_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFS5015004.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(5048..6301)
                     /locus_tag="ACG81A_001642"
     CDS             complement(5048..6301)
                     /locus_tag="ACG81A_001642"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5015005.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6419..7768
                     /gene="gdhA"
                     /locus_tag="ACG81A_001643"
     CDS             6419..7768
                     /gene="gdhA"
                     /locus_tag="ACG81A_001643"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFS5015006.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7880..9226
                     /locus_tag="ACG81A_001644"
     CDS             7880..9226
                     /locus_tag="ACG81A_001644"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFS5015007.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9534..9920)
                     /locus_tag="ACG81A_001645"
     CDS             complement(9534..9920)
                     /locus_tag="ACG81A_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFS5015008.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3179 a         1800 c         2039 g         2929 t
ORIGIN      
        1 tttagtgaaa tcacgaatta aaaccagaaa atattttgta aaaagcaaga ggttgtgaag
       61 aagctgccct attctattac cgtagatgca agcttttgaa cactgtttat tcgaaaagag
      121 gagcagcgac aataattttg tcgctgctcc ttttaaattt attttgtcat tcggaagaac
      181 ttgtggtcgt tccgtgaagt tcttggtacg tttgcgtttt ataaagatca acagttgatt
      241 gtccgttgtg ttcactaaag acactagttg acttgtcacc aagagatttt tcagcttctt
      301 ttaatttctc taagccattc ttgtagtcat aatcactggt atcaattggg tctaatccac
      361 tattcgtata gaaccgaaga agatcaccat tattgatttg gtcagaaaca gctaactgtt
      421 tatcaacttt ttctttcagt gaatcaattt cattctgtac ttcctcagta ggttcagtaa
      481 tagcttcacc agtttcgtta ctatagatcg taccaccgta gtaagtatat tttggtgtaa
      541 caaaatcacc atcacggaaa gaaacaagct gttcatgatc tttcgagaat agatcttgtc
      601 ctaactgaat ataatttttt gtatctacac ctagtaagtg aagtaatgta ggcaaggcat
      661 caacttgtcc gccgtaagtg tgattgattc cgccctgtgt ttgtccaggg atatggatca
      721 tataagggac acgctgcatt tgtgcattgt cgtagtttgt ccaagtagaa cttgtttttc
      781 caactaattc agctagattt ttatttcttg aattcgagac accgtaatgg tcaccgtaaa
      841 ggacgatgac tgaattatca tacaagccgc tttctttcaa ataattgaag aattcttgta
      901 cggcggtatc taaataattg gctgtcgcaa agtaaccgtt gatcgtttca tcatttgtat
      961 tggctaacgg gaatcctgct tcgtcattgg tgaattttga ataaggataa tggttagata
     1021 cagcaatgaa ttttgaataa aatggttgtt gcagacgttc caagtattgt acggattgtt
     1081 ggaaaaatgg tttatcatgc aacccgtatt ggaacgaatt gtcatcatta acatcataat
     1141 aagaagaatc aaagaaataa tcatatccta aattcttgta tgtctcgttt cgattccaga
     1201 atgtaccagc gttcccatgg aataccgcac tagtgtaacc ttgtgtttga cttaaaatat
     1261 caggtgcagc ttggaaagta ttttttccgc ctaattgtgt gaacaaggag ccttgattca
     1321 agccaaacaa tgaattttcc atcagcgttt ccgcatcgct tgtttttcct gcttttactt
     1381 gatggaaaaa gttgtcaaaa ctgaacgtgc tgttgctgtg gtaaagacta ttgatgaaag
     1441 gtgtaacttc atgctggaga ccattctcat ctgtcaattt ataatcgatc aggaattgct
     1501 ggaagctttc taaatggata taaatcacat tttttccttt agcgagcccg aacaaatcgt
     1561 catttggtgc tgcataatgt tcttttacat aattttctac ttctttcata tcattgggac
     1621 ttgcttctgc ccgcacttgg tttgtctggt aagtttgaac accgtcataa accatgaaag
     1681 cattcaagcc aagatatttg accaaataat cacgagagaa tgttcgagtc agcaattctg
     1741 gacgatcagc ttccgccata aaaagatttc ctgagaaaat cataactgct aatgtagaga
     1801 tcgcaaaagc cattcttgct cggatcgggc gagggtccaa cttgattttt tttgcaatca
     1861 gcagaacagt caagataata aaatcgatcc aatagatcac atcatatggg cgaaacagtc
     1921 gaatggcact ttctcccaga ccactcgcta ctttcccagc acctaaaatc gtgttgatcg
     1981 taatgaagtc cgtgaattct cgataataag tgacattcga aaaaacaagc aatgtcaaaa
     2041 gaaaataaat gaccatcatc gtgatataag caactctagg gcgtctgata taaagagcaa
     2101 ttccaagtaa taacaatgta gttgcaatag ggttgataaa caagataaag tattgaatgc
     2161 cactttcgat tccgagatgg aaatcaacta aataggcaaa aatcgttttc aaccaaagta
     2221 aaacgactag taaagaaaaa aatccgagcc tcttattcaa aaaggcaggt gtttttatac
     2281 atttcaagat aatcttcctt tctgcacttc ttaatcacag aagtgtcatg aaaccatgtt
     2341 acacagctca cttattctca tttattttac tagtatgaca atatagtgtc aattgaaacc
     2401 agaagtttat gctgtttcat taaggttttc tttaagcttc atgaaaaaat gagcagtaag
     2461 acaagtccta tgttttttgt tatacttggg aagagaaagc taaagcaggt gaaggaatga
     2521 aagtaaaagt aaaaaaaaga gcaagcaatc gattgaaaaa aggatatcca ctgatccaaa
     2581 aagaagacct tgctcatccc caaaaagaaa ccacgcaatg ggtggaatta gttgattcgc
     2641 aaaaccaatt tatcgcaaaa ggatatctag gaaagcagaa taagggaatc ggttgggtat
     2701 tgagccagca gaacatccca tttgatcaat ctttttttga agaatttttt gtgaagtcaa
     2761 aagaaaaaag aactttcttt ttttcagatg attctactac cgcttttcgt atattcaatg
     2821 gagaaggaga cggaatcggc ggtctgattg ttgattatta tgcaggttat gctgtttttt
     2881 cttggtataa cgaaacgtta tatgcacatc gtgaattatt gattcaagct tttcgtgaaa
     2941 tctacccgga aatcgtagga ggctatgaga aaattcgatt tgaatcagta aatctgcctg
     3001 agagccagtt catttacgga aaagaggcac cggaaccctt gatcgtaaaa gaaaacggag
     3061 taaaatttgc cgtttattta aatgaaggat taatgactgg gatcttttta gaccaaaaag
     3121 aagtacgggg atcattagca gaaggtttcg cttctgggaa aacagttttg aacatgttta
     3181 gttataccgg cgccttttct gttgctgccg caatgggagg ggcaatcgaa acaacaagtg
     3241 tcgatctagc aaaacgcagt ttaaaaaaaa cacaggaaca gtttgaagtg aatggtttgg
     3301 atgtcgatca gcaaaaaatc attgttatgg acgtattcga gtatttcaaa tacgccaaaa
     3361 gaaaacaatt tttatatgac atgattgtat tggatccacc aagttttgcc agaaataaga
     3421 aaaaagtctt tcgcgtagca aaagattatg gggaactggt caaggatagt ctggatattt
     3481 tagcagaaga cgggatactt attgcgtcta caaatgctgc gaatgtctcg atagaacaat
     3541 tccaaacaat gattgaagag gaattccatg ccaaaaatgt ttcttttgaa caacttgctt
     3601 tatatcgtct gccaaaagat ttccagacga cggatacctt tttagaaggc aattatctga
     3661 aagtgtttgt ttatcaaatt aaaaataacc attagaagga gcgatgcaga atggtttggg
     3721 taaagggatt gaatatagga agcggtagac caaagatttg tgcgcctctt atcggggccg
     3781 tccgagagga agtattacgt gaagcagttc ttgcacgtga agcaggggcc gatttagtcg
     3841 aatggcgagt cgatcattat cgtgaagttt tttcttttga tgaagtagca gaaacattgg
     3901 ttcttatcca tgagatgctt gatggactgc cgttattatt tacgtttcgt accttagctg
     3961 aaggcggaga acaagacata tcgatccgtc agtatcgcga cctttatgaa catgtgatac
     4021 acacaggatt agtagatatg gtcgatatcg agttgttcaa agtagaaagt ctaggaaaaa
     4081 gactgataga ggaaatcaag tctttaaaca ttcctttaat tatcagtagt catgatttta
     4141 aagaaacacc agcagaccct gtcttgcttt accgattgaa tgttatggaa cattttggag
     4201 ccgatatcgg aaaactggca gtaactccca ataatgaacg agatgttttg cggttgatgg
     4261 agttgactag acgggcaaat gcttttgtct ccatgcctat tattacgatg tccatgggaa
     4321 atcttggtaa aatttctcgt ctggctggcg gaacgacggg ttccgttatg acatttggtt
     4381 ccttaagcag cgattctact tctgctccag gacaattacc ggtcaaagca ttgaagcaaa
     4441 ttattcaatt attaaatgaa ccgttgtaga acaagtttga ggtgatattt tttggcaaag
     4501 aaaaaaatag taaaaacgaa tgcaatccgt atggtcgaac aaaaaaagat tccctataca
     4561 gagcacgaat atgagtggga cgaaagccac ctaagtgcgt catcggtcgc tgaacagctt
     4621 ccagaaagcc aatctcgtat atttaaaaca ctggttgctg tgggcaatgt gacggggcct
     4681 cttgttgctg tgataccggg agaagccgaa ttgaatctaa aaaaattagc aaaagtttca
     4741 gggaataaaa aagtagaaat gctccattta aaagatttgg aagcaacgac cggatacata
     4801 cgtggtggct gctcgcctat aggtatgaaa aaactatttc ctacttattt agatcaaatt
     4861 gcagaaagct atgaacaaat cattgtctcg gctggcagaa gagggctaca gatggagtta
     4921 gcacctcaag atatctgtgc attaacgagc ggacaatttg cagatataaa acaataaatc
     4981 atacgtataa aagaaagaag gaggccggga tgttgtttgt ccagtctcct tctttctttt
     5041 ttatacatta tgctctttgc ccggcattca ttcctgctac atgccctgta gcgaaagccg
     5101 ctgtgatatt aaagccgcca gtataaccat tgacatccag taattctccc gcaaaataca
     5161 gaccatcaat gattttactc tccaatgttt taggataaat ttcctttaga gaaacccctc
     5221 cgccagttac aaatgatttc tcaataggaa aagttttgtt gattgatagc ttaaactctt
     5281 tgcatagttg aacaaaatct tgaatttgtt tttgagaggc ttgttgtcct gtcagatgat
     5341 ccatttcaag ccgctcaaga aagaaaacta acaatcgctc aggcaatata ccatgccaag
     5401 cattgaccaa gttctttttg gttgctttac ttttttctgt caatatatga ataagctctt
     5461 gtttggtttg tgtagggaaa caatccagag aaagtgtcac tggttctccc gttttctcta
     5521 attctttatt gacaaaactt gaacaacgca gtgcagccgg accagaaata ccgaaatggg
     5581 tgaaaagtaa atccatcgta tgttcagtca atacacgacc ttttttattt aaaacgcgta
     5641 aagtgatgtc ttgtaaagaa agcccttgaa gtgttttctc ctgaatatac ggttcatccg
     5701 atatcaaggg ggactctgtc gcataaagag gcgtcaccgt gtgacccact cgcttcacga
     5761 tcttgtaccc atctccagta gaaccagtag atggataagt ccgtccacca gttgttaaga
     5821 taatggcagg agcatggaac tcttcgaatt ccgtgcgtat accgtaaatt ttatgttctt
     5881 tgtggatcaa tttctctact cgagtagaga atagaagcgt cacatctagt tctttcagac
     5941 gattgagcaa tgcttcgata atcgtttttg atttatttgt tacaggaaac atccgtccat
     6001 gatcctcttc ttttaaatgt acgccttgtg attcaaagaa gttcatgata tcgaaattat
     6061 tccattgcgc aaacgtacta tataaaaact ttccatttcc aggaatatgt gcaatcaaat
     6121 catcaacagg acgattgttc gtaacattgc accgaccacc acctgtcatc agtagttttt
     6181 ttcctgcttt tttgtttttt tcgatcaaga gtacgcgtgc gccttgttca gctgcactga
     6241 tcgctgccat catcccgctt gttccagcgc cgactacgat cacatcatac agtttttcca
     6301 ttccctcacc tcaaggaaag tgtagcatat ctgaagaaag aaaacagaaa aaaaatgttt
     6361 tgagccaaaa gagaaaatga gctaaaatga gggaagataa gcaaaggagc gtggaacgat
     6421 gacaaacgca acagaatatg ttcaagcaat tcaagaaaag ctacatcaaa aggatcaagg
     6481 gcaagaagaa tttttacaag caatcgatga attcatgccc acggttatgt cttttttgga
     6541 cactcatccc gaatatatcg aaaagaatat cttagaactt ttaacggaac cagaaagagt
     6601 tattcaattt cgagtgcctt ggcaagatga tgaaggaaac tggcgagtaa atagagggta
     6661 ccgtatccaa tacaactcag ctatcggccc ttataaaggt ggattgcgct tccatcctag
     6721 tgtcaatcta agtatcttga aatttttggc ttttgaacag attttcaaga atagtttaac
     6781 cggtctgcca atcggtggag gaaaaggcgg aagtgacttc gatcctaaag gaaaatctga
     6841 taatgagatc atgcgttttt gtcaaagttt catgctggaa ttgtcaaaac atatcggccc
     6901 atcgattgat gttcctgcgg gagatatcgg tgtaggagct agagaaatcg gctatttgtt
     6961 cggcgaatat aaacgattga aaaaatatga tactggtgtg ttgacaggta aacctttgga
     7021 tttttgggga agtaagatac gtacggaagc tactggttat ggattagtgt attatgtcaa
     7081 acatctattg aacgaagaaa aagattcatt taatcaaaaa acagtttttg tttccggaag
     7141 cgggaatgta gccatttatg caatcgaaaa agtgcaagag ctaggcggga aagttgttac
     7201 atgttcagat tctagcgggt acatctatga tccagaagga atagatgtga aactattgaa
     7261 agaagtcaaa gaaatcaata gacaacgttt gacggaatac gcaaaccaac gtccttctgc
     7321 tatttatcat gctggagaat ctgtttggac attgaaagaa aaagcggata tcgctttgcc
     7381 atgtgctact caaaatgaaa tagacgaaag cttggccgaa atcttagtag aaaatgggat
     7441 caaaatcgta gcagaaggcg ctaatatgcc atgtacagta aaagctgttt ctgttcttca
     7501 tgaagcaggc gtgtggtatt gtccaggaaa agctgctaat gctggaggag tagcagtatc
     7561 tgctttagaa atgagtcaga acgtccagcg attgacttgg gaaagagagc aagtggatga
     7621 gcagctggat caaatcatgg aaacgattta ccatacgtgt cgccaaacag cagccgagta
     7681 cggcgacgaa aaaaatctgc ttcttggagc aaatgttgct ggatttgaaa aagttgccaa
     7741 agcaatggca gcagaaggtt tagtttgaaa aatgaaactt tcttggaaat attgtcagca
     7801 ttttgtttat ttgcttgaaa aataataaaa aaaaacgtaa agtattacct gtagagtaaa
     7861 cctataagga ggagaacaaa tggcacacat tcattttgac tattcaaaag tagcaccgtt
     7921 tgtcaacgaa caagagttag ggtacatgca aagccaagta acagcagccc acaacgaatt
     7981 acgggaaggt actggcgcag gaagcgactt ccgcggctgg atcgatttac caacgaacta
     8041 tgacaaagaa gaatttgcac gtatcaaaga agcagctaaa aaaatccaat ctgattctga
     8101 agtattagtt gttatcggta tcggtggttc ttatttaggc gctcgtgcag cgatcgactt
     8161 cttgaatcac acattcttca atttattaga tagcaacaca agaaaagcac cacaaatctt
     8221 ctttgccgga aattcaatca gctcaacata tattgctgac ttgatcgaag taatcggtga
     8281 tcgcgacttt tcagtaaacg ttatttcaaa atctggaaca actactgaac cagcgattgc
     8341 gttccgtgta ttcaaagagc ttcttgttaa gaaatatgga caagaagaag caaacaaacg
     8401 tatctacgct acaacagata aagcaaaagg tgcagttaaa gtagaagcag atgcagaagg
     8461 ttgggaaact ttcgtgattc ctgatgacat cggcggacgt ttctctgtat taactgctgt
     8521 tggtttgtta ccaatcgctg ttagtggtgc agatatcgat gcattgatgc aaggtgccgc
     8581 tgatgcaagc aaagcatact caagcgacaa actagaagaa aacgaagctt accaatacgc
     8641 agcaatgcgc aacattcttt accgtaaagg caaagtaact gagttgttga tcaactatga
     8701 accaggaatg caatacttct cagaatggtg gaaacaattg tatggtgaat ctgaaggaaa
     8761 agaccaaaaa ggcatctatc cttcaagcgc aaacttctca actgatttgc actctttagg
     8821 acaattcatc caagaaggac gtcgcaatat ttttgaaaca gttgtgaagg ttgaaaaacc
     8881 tcgtaaatca atcacaattc ctgaacaagc agaagattta gacggtctag gctacttgca
     8941 aggaaaagaa gtggacttcg taaatacaaa agcatttgaa ggaacattgc ttgctcatac
     9001 agacggtgac gtgcctaact tgctagtcaa aattccagaa atggatgctt atacattggg
     9061 ttatacaatg tacttctttg aaattgctgt aggtatttca ggttacttga atggaatcaa
     9121 cccattcgac caaccaggtg tagaagctta caagaaaaac atgttcgcac ttctaggcaa
     9181 accaggcttt gaagatcttg ctaaagaatt gaacgaacgc ctgtaagata agggtttttc
     9241 gctagattta aataatcagt gatagaaaca tgttcgcttt aaaatgaaaa ctggtaccca
     9301 tttaggtact caaacatact gcatcgttta ttcagaataa ggggctgtga caagacgttt
     9361 gtcacagtcc cttgtttgta tagaaatcga ttgattgcct tgctctttag tttgttttag
     9421 gactttaaga acagtgaggc tgacttcgaa ggttaattct gtttttttgt tttctagaat
     9481 cagtaagcaa aaaagcactc acttcgagtg cttttttgcg atatattctt agcttaagat
     9541 aggcctaatt tttcttttac agatgagggt accttatcgc tggtaattac ttttttctct
     9601 atgatccgct tttcgcttgc tttcaactgc aagatcgttc cttcggggta agcagaacca
     9661 ttggcagagt caccagaagt aatgacatcg aaatctaact gtcttttttc accgttttca
     9721 gcataagcat ctacgttgta tttaaaacct cttccaatta cttccccatt atctgctttg
     9781 atatcagttt ctgcaggcat tggagctttg actaccgcat aataatcttt acctacataa
     9841 gtagactgat agtagtccca acctttccat cctataaaag caatcaatat gataacgatc
     9901 aaccctaaaa tttttttcat gaaagatctc cttttttatt tttatta
//
LOCUS       DAZULY010000103         9944 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000103 DAZULY010000000
VERSION     DAZULY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.104"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..613)
                     /locus_tag="ACG81A_001646"
     CDS             complement(<1..613)
                     /locus_tag="ACG81A_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFS5015009.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
     gene            814..2027
                     /locus_tag="ACG81A_001647"
                     /pseudo
     CDS             814..2027
                     /locus_tag="ACG81A_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2206..2541)
                     /locus_tag="ACG81A_001648"
     CDS             complement(2206..2541)
                     /locus_tag="ACG81A_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS5015010.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(2525..2788)
                     /locus_tag="ACG81A_001649"
     CDS             complement(2525..2788)
                     /locus_tag="ACG81A_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFS5015011.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(3248..4051)
                     /locus_tag="ACG81A_001650"
     CDS             complement(3248..4051)
                     /locus_tag="ACG81A_001650"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5015012.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(4029..5153)
                     /locus_tag="ACG81A_001651"
     CDS             complement(4029..5153)
                     /locus_tag="ACG81A_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5015013.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(5169..5984)
                     /locus_tag="ACG81A_001652"
     CDS             complement(5169..5984)
                     /locus_tag="ACG81A_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5015014.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5974..6846)
                     /locus_tag="ACG81A_001653"
     CDS             complement(5974..6846)
                     /locus_tag="ACG81A_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5015015.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6931..8220)
                     /locus_tag="ACG81A_001654"
     CDS             complement(6931..8220)
                     /locus_tag="ACG81A_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5015016.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(8364..8876)
                     /locus_tag="ACG81A_001655"
     CDS             complement(8364..8876)
                     /locus_tag="ACG81A_001655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015017.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(8943..9086)
                     /locus_tag="ACG81A_001656"
     CDS             complement(8943..9086)
                     /locus_tag="ACG81A_001656"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS5015018.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
     gene            complement(9569..>9944)
                     /locus_tag="ACG81A_001657"
     CDS             complement(9569..>9944)
                     /locus_tag="ACG81A_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5015019.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVWLSVRTAFFVG"
BASE COUNT         3054 a         2110 c         1610 g         3170 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tgactggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttccatga gaattgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ccttccacaa tcatggcaga
      361 gaaaacgggt acgttttaat tctaaaatag ttaacttttc tttgactttc aatatctgag
      421 tgcgtgtcgt ataggaaccg ttgcggataa tacgaaccga tcgacagtgt tcacaatagg
      481 aaggagagtc atccaatttt cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag
      601 tatgattatc cattgagaga ctcctttggt aggatgacgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc
      721 gattttccac caacacaaaa tattatagaa gcaaaatcaa ttctacctta gatgttctta
      781 tatcactcta tcagtcctat ttgacaaaga gccaagaaaa agacgttggt cgtcgatgct
      841 gttctctcgc ctactcctcg tgcttgtaga agttgtggtt ctactgtggt agatggaaac
      901 gggaaagcaa ttatagtgaa aaatgggaag aaggaaacga ttgtccgttt tgaacaatac
      961 aatcatatgc ctttggttac gcgcctaaaa aagcaacgct atacctgtaa aaactgtcgt
     1021 acccattgga cggctcaaag ttattttgtc cgactcagcc attcaatcgc agatcatgtt
     1081 cgatataaaa ttgcttcttt actgactgaa aaagtatctt tgtcttttat tgcgaaaagt
     1141 tgtcaggtat ctttgactac cgttactcgt acattgaaag agtttaaaag ctatttacca
     1201 aagccttcta agaagattct ccccagagta ttgatggttg atgaatttcg ttcgcacgct
     1261 tccatagaag ataaaatgag ctttatttgc gcagatggtg aaacaggaaa cttaattgct
     1321 gtcttgccta cgcgtaaatt acctcgatta acaagctatt tcttaggctg caccaatcca
     1381 gaagaagtag aattcttagt cacagacatg aacgccgctt acttccagct taccaaacgt
     1441 gttctgccaa atgcgaaagt agtgattgat cggtttcata ttgtcaaaca catgaatcag
     1501 gcactcaatg agttgcgtat ccgtgaaatg aatgagcttc gtaaagcagg acagaaaagt
     1561 caggcagaaa aactgaaaaa gaactggcgc tttctgctaa aaaatcgtgc aagcatcaac
     1621 cattatgagt acaaaacatg gaaaagtttc cgagcaccaa aatacccatt tctcactgaa
     1681 gcaatgatga ttgatcgatt gcttgagttt tctgcgcctc taaaggaggc gtatcccttt
     1741 ttcatgaatt agtcgaagcc tttcgagaca aagaccctga cttatttttc tccttattgg
     1801 cagaacttcc cgaaacgtta gatgacggct ttcgggagaa gcttcaaaac cttctgacct
     1861 atgaagaagg tatcaccagc gcaatgatct atccttattc caatggaaaa attgaagcga
     1921 agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca ttcgagaaca
     1981 tgagaattag aatctttttg atcaatcaat taatcaatgt aagataacaa aaaatctgaa
     2041 ccagaatgtg gttcattctg acccggattt gattttgcac aactcatcag tccttattga
     2101 caaagagcca aaaatctgag ccagaatgag gttcattctg acccagattt gattttgtac
     2161 aacccatcag tccttattga caaagagccc ttctttttag tattattata attcttccat
     2221 gcgttcttcc agtcgctgta aatcgcttgg acttaattcc ccaatttttt tccatttgac
     2281 ctttttgttg tttttgggta attcgtttag agttcctacg tcaatccaag attctttttt
     2341 tagtcctgcc tgtttccaat cccttatttc aacgtatttc agctttattt tatcggattt
     2401 agtatgatat tttgaagtaa ttgacagaaa cgaaattgtg tccagtccat tttttagaat
     2461 atagacaggc cggcgtttac cgccctctgc agctgtgaaa gcaacgtaga cggttgctat
     2521 atcattagta ttcatcttca ttgaaccatt cttcaatttt ttcgtcagtg tctagttttt
     2581 ggacaggcaa tttttccgct aatctttgaa tattatggat tcgtttactt ttttccgaca
     2641 attcaataga aaatgggatt tttccttgct caacaatttg cttataaaaa acggtgacgg
     2701 ctgttttggg gctcatgcct agttcatcaa taatgctttc tgcttcattt ttcaattttt
     2761 cgtccataga aatttgtaac ttagccattt tctccacctc tttctgacaa acacattata
     2821 tcacggaaag agttcaaatg gaattaatac aactactttt gaactccttg aaatgcagtg
     2881 tatttatcta aatataagga aaggatatag tgctaaatgg ccttgataga tagaaaagtt
     2941 tcaaaatccg ccacagcgat aattgaagcg aacactcaac gacacacaga acaggaaaag
     3001 actcctaaaa gaaccaaatt aactaggacg tgtctgaaaa ctccacaaag atgtggtatg
     3061 ctctggtaaa aaacgaatgg cacaaccacg aagaccgata tggttcttgg aaaactttct
     3121 ccaccctttt tgtaattgga gggatagtgg tttattgata gcgattttcc aagttcttca
     3181 agtggaacca gacattgagc accacggcta aaaagtgaat gaagtcttta tttttaatca
     3241 ataattttta atgattacca gtaattggtt ttttcatgcg ccctatttct atttgatgtg
     3301 gagttaaata atgctgataa gtaaaaattt ttccgtcaat taaactgcca ctagtgaatc
     3361 ctgttctatt ggtaaaaaga gaatagtctg ggtattcatc tacaccaaat gataaagatt
     3421 cagccataaa ctgtttttta ccagcaaatt cagcactgct accttgatgg atatgtccta
     3481 caaaaatacc taaaatatca ctttcggcaa tcaattcagc aaaaccttcc ggaacttcag
     3541 ttacaataga tggatcttcc cacgacaatg gatgatgaac caataaaatc gttccctttt
     3601 cagcaggtgt acccaaccac tcttgtaatt gaatcatctg tcctatcgat atttttcctt
     3661 cgtgctgggt atcttgagca gaatccaagg aaataattcg taacccattt tgattcgtac
     3721 aatggatata ctctcccgtt tcattgcttt caacactcat tccttcaaag aaaacactgc
     3781 gacgatcatg attcccacga caaaaatagt aaggtgtggt tgggaaatat tgatccaaca
     3841 ttttccgtaa gacttgataa tcagaaatcc ccccttcatg tactaaatca cctgttaata
     3901 gaataaaatc gtacactgtt aacgagagtt cttgtaacaa tttttccaat gccaactgtg
     3961 gatttttaat tgttggaaat agttcagcag taaacaagtt agtttgatag ttttctctaa
     4021 aatggatatc agagagatgc aaaaagcgca tggctattcc ctctttcttc aataaaataa
     4081 tcaggatatc ctaaattttc ttgcgtctga taatcaaaaa aatgcattct ttcaggttct
     4141 atgcggagat tgactgcatc accaatctgc caagtaggtt tttcattaag gacaatcatt
     4201 tctataccat cgactttaac ggtgtacgca taactttgac ctaatagctc acgatacttc
     4261 acttgaccgt gtaaatcgct tgtttctttt gaaaaggtta cgtgttctgg tcttatgcct
     4321 aaatacaatg agccactaac ctgaagaagt tgtttttgcc aagctggatt caatgttaaa
     4381 aattgactgg ctacctttaa tgttccttga tcataagtaa ctggcataat attcatgcct
     4441 ggcgaaccaa taaaatttgc agtaaaagta tttttaggtt tcgtataaac atttttcggc
     4501 gtatcaatca tctgaattct accttcattc aataaaacaa tccggtcagc taacgtcatc
     4561 gcttcaattt gatcatgagt gacgtagaca aaagtttgtt tataacgttg atgaattttt
     4621 accaattctt ttcgtgcatc caatcgtaaa cgcacatcca aatttgataa cggttcatct
     4681 aataaaaaat aattactccg tttaaccacc gcacgagcca atgctacacg ctgccgttgc
     4741 cctcctgaca aatctttagg aagtcgatct tggtatggcg ttaaacctaa catcttcaaa
     4801 gcttcttcca aacggtgctg aatgtcagct gtcttaattt tattcgccct taatgcaaaa
     4861 ataatattct cctttacagt catgtgagga tataaagcat aattttgaaa aaccattgcg
     4921 atctctcgtt ctccacaagg tagcttattt gctagttgtg tacctaaaaa aagctctcca
     4981 ctcgtaattt tttccaaacc agcaatcatt ctcagcaaag tagattttcc tgaaccagat
     5041 ggtcccagta acacaattcg ttctccagcg gccacagcta aatcaatttc ttttaaaatg
     5101 gtatgttctc catatttttt tccgatattt cttaattcaa gcgtttctcc catgatttgt
     5161 cctccaattt atttaatacc tgcagaaaca aaagtcccga taatatattt ttgaaaaatt
     5221 gcatacaaga ctaacggtac aaacattgta attaaactaa tcgccattgc tacggtaaaa
     5281 tttgtcccac cttcagaact aatataggtc tgcatcgcca acggcaaagt ataattatct
     5341 actgtccgta aaattaatac cggccaaaca tactcattcc aagaggtaat aaaaaaccaa
     5401 ataccggtag aagtaatcgc atgtctagta agaggaaaaa caattttact catgacggtt
     5461 gtttgtttga tgttatccaa tgccgccaca tctagaaggc tcactggaat ttcccgcatt
     5521 gtttgcgtca ataaaaaaat gcccatcgca tcagcaaatt gtggtattat cacaccccaa
     5581 gtagtatcaa ttaaattaag ttttgcaatc aacaaataat taggaatcat cgcaacagta
     5641 aaaggaatga acatcgtacc aatgatgata aagtaccaga ttttcttccc tttgattcgg
     5701 caataagtaa aactatatgc cgcaaaaaaa gctacgccta acttaacaac cgttataatt
     5761 gtagcaataa aaaaagtatt tctcgtaatt ttgataattg gaagattttc gaacaaatag
     5821 caaaaatttt ccaaggtaaa cggctgtggt atcaaactag taatcgtctg ataggcatct
     5881 ttcaatggct taaaagaatt ggccataata aacaataatg gatagataat aatgattatg
     5941 atgaacataa ataagagatg tggcaaccag cgtttaattt tcatagtaaa cccccttttc
     6001 cacaaattta aatattaccc ctaagaaaac agcaaatatt aataaagtta aaactgaaat
     6061 tgctgaagat tctccgactt tatacatttc aaaagccttt tgataagcat aatagatcac
     6121 gtttgcacta ccattattcg gtccaccacg agtgatgatc ttaatcggag taaacacata
     6181 ctgcagacct tgaacaatgg tcatcaccaa taaatatagt cctgtcgaac tactcatagg
     6241 caacacaatt gtaaaaaaaa tgcgccagat gggtacacga tccaacttag ctgcttcgat
     6301 catctcttga tcaattccca caattgctgc aaaaatgaca atgaaattat aaccaaaaac
     6361 tttccaagca gtaatacctg caatcacagc aattacccaa ccttgtaaat tcatccacat
     6421 aggcagtctt aaaccaaaat ttcccaacaa gacagcaatt ggtcccgata ccggatttaa
     6481 tatccatgta aaaaccatcc ccgccactac caaagaaatc acagctggaa taaaaaataa
     6541 tggtttatac aaattctgcc ctttttctat aactattcct aataaaaagg atgtaatata
     6601 tgggaacaca cagctcagca agacaaatag aacggtatag gacaaagtat tcttcaacac
     6661 tttatgaaag actggatcag tcagcacctt tacatagttg gcaaagccaa caaattcttt
     6721 agtgggggcc accatattcc agtcaaaaaa actcaaatag atcgtataaa tcaatggata
     6781 aaaaacaaac acgcccaaca aaataattgc tggcgccaga tacaaataac ctacatagcg
     6841 cttcatttcc gacagcctcc tcatccttat gtcaaaagca gacgaccttg tttttccttt
     6901 cttacaggcg ctgtcgtctg cttgctttgc ttatttgagt aactcattaa tttccttttg
     6961 agtagctttc atcgtttcat tgacggaatt acctgataaa attttatccc gcatatccaa
     7021 caatttttgt tcagcttcta atccagcatc tccaggaaat gaactccaac tgaccatctt
     7081 atccatctga tcaattgctg gtatcatcat ctgattttca gctaagaaag cttttaatcc
     7141 attttgggcg tctgcaacat ctttacgtgg tggtaaataa ccagtacctt ctgtccattt
     7201 agctaaagat tcaacactat aaagatattt taagaaatcc caagttgctt cctttttctt
     7261 atccgtttgt gatgtaacag ctaacattgc gccaccagca ggtagtttaa cttttttccc
     7321 ttcccaactt ggtgactcaa ctgctttagc agtaaaagta ctattttttt gaatgttgtc
     7381 cctttgagca atagtggtat agaccatcgc gacgttacca tcaataaagc tttgcacacc
     7441 ttgttcccac gtagtgtgta atgccgtctg gtcttttacc atcctttgat ataattcata
     7501 agcagcgata ccatctttag aagcaaaggt agctttgcca tcttttatta cacttgcccc
     7561 attactttct aaaattgctt gttgtgccca attatcagca ggttcttgaa tatacacgcc
     7621 atactttcca gttttttctt ttattatctt agaaaaagtt tgaacttctt cccaagtttt
     7681 tggaccattt tcatccaagc ccgctgcttt taatatatct tgatttaaat ataaaaccgg
     7741 attactaatg gagtaaggaa tacccacttg tttaccatct ttttcagaca gtgccatgat
     7801 attgtctaaa aaatgatctt taaaataatc agcattatcc ggatccactt cttctactaa
     7861 agattgagga ttagtatatt tgaaattttc tgcaaaataa tctttaaaag cccatcccac
     7921 ttgtactacg tccggtattt ctttggaggc aacagaagct tgtaaatttt gcatcaagcc
     7981 tttatacata tcaggattat attttgctac aaccttcact ttgtcttgag attgattgta
     8041 tttatctact aattcattca ctgtttttcc acctagggta tccgcattaa catgccaata
     8101 ctctatcgtt acacgaccct ccgatttttt tgttgtcttg gtattcccac taccacaagc
     8161 tgccaaagag atgacactta aaacagacac aacccctata acaagatttt tctttaacat
     8221 aacattaact tcctcctaat ttctttatgg ccatttttct aatcaagaaa tattatgaac
     8281 taccaatgta aatttctagt taattttctg taaataattc gaaatttgtg catttcgggg
     8341 tacagggtca ctggacatat ccatcaagct aagaataaag cccccatatg gtcatgtatc
     8401 ccatctccaa atctcttaac tcctctgttt tgattataaa atagtttatt ctgtgctcct
     8461 ttcttccata aataggccca tgcacaacgt gaggttatat tataaacacc acagcgcgca
     8521 aggtactcaa tcgtttctag ttcattgact gtccatacag aaaatgtcaa tcctagctgc
     8581 tgccccactt cacgggccaa cttagttaac catccttcat gaatattgac gatttgaacg
     8641 ctttgatagg ctaatttctg taaggtatcg atcaaatctg cttcggtaat atccccaagt
     8701 tttttccctt cgaactgatt ttcaatatta taaaatatat gctttcccca aatcgcagcc
     8761 caagataatt gtacatagcc actaagaaca atttgtggcc agatttgata ggtttgtgcc
     8821 agcgaaaaaa cggccatttc caatccctct tcttttaaat cacagttaat ggtcatggtt
     8881 gccttctcat tggccactgc ttgaaacacc tgctcaaggg ttaccgaatc tgggacaact
     8941 tcttacctgt catgggctag tattagcgta ccatctgttt gctgacgcac atctacttca
     9001 aaacaaagct ggcgatccaa acaaaagcga agaaatgccc agctattctc ctccgtattg
     9061 tcactaccag agtgtgcagt gatcatggtc attttgaccc ctcctttaat tccgtgattt
     9121 tctcccacac gtccactata ccgatgaagc gttaatttct tgttaatttc ttgtaaattc
     9181 cctgcgtttt ttcagtaaac tagttgtaaa tcttgcatgc tccttcctac gaactcatga
     9241 atcgtcgatt ttgcccttct agctagtttc accaattaag tagttctaca ttccccgctt
     9301 ttaacctact acaccttttt ttgttttcag ctattccttc ttcttgctga aagtccttga
     9361 ggttctttct tacaggatac catcaaggtg ttcaatggct tattcccatt gaatgaccga
     9421 gacttactat cagatttctg ctcgttgaac caagtctttg atacaggatt tcttccgtca
     9481 tcttctcaca aataccacct tgcgtttctc tagcgattgg tcgatgtgca ccctcccagt
     9541 ggactgtcac tacccctttt tgctatgcct agcccacaaa aaaagctgtc ctcacagaca
     9601 gccagactgt agacaaaagc tgttccaaac tcgtaactcg agcttggaac agctttttat
     9661 ctacttcgtt tcgaattttt gaacaaaaaa ataaaggctc cttgcctttc catagccagg
     9721 tggctaattt ctttaaattc attgcagcaa acactagcat cgtatgcgtc gcgacttttt
     9781 ccaagcctcg atacttggtc caacgcatcc catgcttttc ttttgcatcc gcaaatattc
     9841 gttcaatcgt ttgtttccgt tttttgtaaa ttgatttatt cagatctgtt aggcgaagat
     9901 gttccacttc atccatcaga tctccccaaa catgtctcgt gatg
//
LOCUS       DAZULY010000104         9933 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000104 DAZULY010000000
VERSION     DAZULY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.105"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            116..3343
                     /gene="fss3"
                     /locus_tag="ACG81A_001658"
     CDS             116..3343
                     /gene="fss3"
                     /locus_tag="ACG81A_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFS5015020.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3456..3770
                     /locus_tag="ACG81A_001659"
     CDS             3456..3770
                     /locus_tag="ACG81A_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5015021.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3783..4157
                     /locus_tag="ACG81A_001660"
     CDS             3783..4157
                     /locus_tag="ACG81A_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5015022.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4167..4502
                     /locus_tag="ACG81A_001661"
     CDS             4167..4502
                     /locus_tag="ACG81A_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFS5015023.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4585..5925
                     /locus_tag="ACG81A_001662"
     CDS             4585..5925
                     /locus_tag="ACG81A_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS5015024.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            6003..6677
                     /locus_tag="ACG81A_001663"
     CDS             6003..6677
                     /locus_tag="ACG81A_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS5015025.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6670..6834
                     /locus_tag="ACG81A_001664"
     CDS             6670..6834
                     /locus_tag="ACG81A_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015026.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            6910..8052
                     /gene="dcm"
                     /locus_tag="ACG81A_001665"
                     /pseudo
     CDS             6910..8052
                     /gene="dcm"
                     /locus_tag="ACG81A_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            8042..8332
                     /locus_tag="ACG81A_001666"
     CDS             8042..8332
                     /locus_tag="ACG81A_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015027.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8591..9775
                     /gene="mobT"
                     /locus_tag="ACG81A_001667"
     CDS             8591..9775
                     /gene="mobT"
                     /locus_tag="ACG81A_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS5015028.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9772..9909
                     /locus_tag="ACG81A_001668"
     CDS             9772..9909
                     /locus_tag="ACG81A_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFS5015029.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         3289 a         1677 c         2245 g         2722 t
ORIGIN      
        1 ttcattctca aatccattct attcagtcaa taaaaatttt aaaaagactt tataaacaaa
       61 gatgttcaag cgagcatctt atttttttgt gcttaatttg aaaggagaac atttcatgta
      121 taaaaaacaa aaaaagaaat tatgtgtatt aactattttg agtttggctg ttcaagcaat
      181 tgttggagca attgctccta cgattacttt tgctgatgaa attacccatc cacaaacggt
      241 aaccgtagaa ctagatttag cacatcaata tgctgttgaa ggaactttta gtgatggtcg
      301 tccaatgtca gaagtcactg ttccgcatta tgcagtgtac aacggcttta aacaagatat
      361 tttctgtatt gaaccaggag tcccgattta caatgagttc actcctggct atgagaaaaa
      421 cccgttacca gatatgtccg ataaagccaa attagtttcc gttttatgga aaaaagtagg
      481 tacagatgca gatactcata ttgtggcaca gaagatgatc tggcaagaag ttaatgggta
      541 tacccttcac tcaataaaac gatcagatgg tagtgccgta aatatcgcag ccattgaggc
      601 aaaaataaat caagccattg acgattatca gaagaaacca accttccata atagtacggc
      661 taaaacagta ttagggcaat cgactactgt gacggatacg aataatctga atttgtcaga
      721 gttcgatgag gttgtggaaa atgccgcaaa cattgattat cgtgtgaatg gtaatcagtt
      781 gataatcacc ccaaatgcca attcaaaaga aagtggtgtg ctgacgctta agaaatctgc
      841 tggtaccgga acacctgtcg cctataaaaa ggctggccaa cagactttaa tggccggggc
      901 gattgataaa cctaatacct acacaatcaa aattgatgtg gaaacggagg gttctttaaa
      961 gatcaaaaaa gtcgataaag aatcgggtga tgttgtacca ggaacggttt tccatttaga
     1021 ctttggaaaa actttacctg caaaagacgt gactactgac aaagaaggca ttgctacatt
     1081 ggatgagatt cctcatggta caaaagtaac cattaccgaa aaatcagtac cggcgcctta
     1141 tacgattgat accaccccca tgactaccac cattaaagca ggcgaaacca tttacgtgac
     1201 ttcaaaaaac gcacgggaaa aagggcaaat cattctggat aaaagcggag ttgaaacagg
     1261 gagtgatctt tggaatgaca attattcctt agctggtaat acgtttgcca ttcgcaaaga
     1321 cagtccaacc ggtgaaatcg ttcaagaaat gaccacggat gaaaacggtc atgcggaaac
     1381 accaaaagag attgctaatg cgttggaatt gggaacctat tatgtgaccg aaactaaagc
     1441 tagtcatggg ttcgtgaata cgttcaaacc tgtaaaagtc gaattgaagt atgccaatca
     1501 aaccgtagcc cctgttacca gtaacgtaaa agggcaaaat caagaagtca ctggcgaaac
     1561 cactttgacg aaagaagaca aagatactgg ggataaagca caaggtaagg cagtctttga
     1621 gggtgctgaa tacaccttgt ttactgctaa ggatggaaaa gctgttaaat ggagtgaggc
     1681 ttttaaacca gagttagcga agggaacgaa aggttctgat gaaacggtca ctttgacttt
     1741 agacgaaaag aaccaagttg ccgttaaaca tttagctatc aacgagtatt attggcaaga
     1801 aacaaaagcg cccgaaggat atagccttga tgaaacaaag tatcctgtat ccatcaaaaa
     1861 ggtggatgat agcgaaaaaa atgccgtgat tacccgaaat gttacggcaa aagaacaagt
     1921 gattcgcttt ggctttgatt tctttaaatt tgctggatca gctgcgggca ctaccgaaac
     1981 tggcttcaat gatctgacct ttaaagtgtc gccattggaa gggaccaatg aaatcacagg
     2041 tgccgaagat gaagcgacca cagcttataa cgagcaatta gggtttgatg gttatggcaa
     2101 gtttgaaaat cttccttatg gggattattt acttgaagaa gtagaagccc cagaaggttt
     2161 tcaaaagatt acgccattag aaatccgttc tacttttaag gaaaacaaag aagactttgt
     2221 gaagagtgag tatgtcttta ccattaccga acaagaccaa aaacaaccaa ttaaaacggt
     2281 gaccgttcct tacgagaagc tgacgaacaa agcattttct gttagtttga accgtttaat
     2341 gctctatgat ttgcctgagg aagaagatag tttaacttct cttgcgactt ggaaagacgg
     2401 aaacaaagaa ttaacttctc tggattctac cgagctagtt gataaattga gttacaatct
     2461 acatgaaatc aaagaagact ggtatgtggt agcgcaagcc attgatgtgg atgcgacaaa
     2521 agctgcccaa gaaaaagatg aaaaagccaa accagtggtg attgcagaaa caagcgcaac
     2581 gttggcgaac aaagagaaaa caggaacttg gaaaattcag cataaattaa ccgccgaaca
     2641 agttttaaat aaaactattg tcttattcaa ctatgtgtat gaaaataagg aagcttttga
     2701 agcaggcgat aagccagtag cgaaggatgt tagcttgaat aaccaagccc aaactgtcag
     2761 ttgtacagtg gaacaccatg tttctatcca aacaaaggcc cacctagaaa acggttctca
     2821 gacctttact catggtgatg tggtggatat gtttgatgat gtgtcgatta ctcatgatgt
     2881 actggatggc tcaaaagaag ctttcgaaac aattctgtat gctttattac cggatggtac
     2941 aaacaaagaa atttggaaat ctggcaagat tgattatgaa gtaaatgata aagaattcac
     3001 caaaactgtg cttgcgaaaa aagtagatac cggaaaatat ccagaaggaa ccaagtttac
     3061 ttttgcggaa atcaattacg ataaagatgg gaatatcaat ggaaaacaca atgaagattt
     3121 gaaagaaaaa tctcaaacct taacaccaaa agaagttcca accatactaa gtacgccgaa
     3181 acaaccggaa acaccaactg ttccaagtgg ctctcaagaa tctagtccca cagtgaagac
     3241 cttccctcaa accggggaga aaaattccaa tgttttactg ttcattggtt ttactttgat
     3301 ttttgcgacg gcgggctatt atttctggaa tcgccggaac taaggtgatg agatgataca
     3361 agtaaatcct aagaaccaac gtgcgcgacc gcctcctaaa aaagtagtcg cgctattttt
     3421 gtatccaaaa ctaacaatga aaagaggaaa agaagatgga attgaaattt gtcgtgccag
     3481 atatggctga aacctttgga aagttaagtt atgccggtga aggagaaatt ataacagaag
     3541 gatatggacg gaatactacg gtgattggtc gcagttatca tttgtattcc agtaagcaac
     3601 gggccgatga tattgaggtg gtagtagctg cggaagccgg tgaaaaggat tttgatcaag
     3661 accaaccgct aaaagccgtg aatccccatt tggttgccaa aggctatgag attgagaatc
     3721 gtgggtttac tgattacgtg ttgtatgttg atgatttagt gaaagcatag gaggaaaaga
     3781 agatgagatt aacagaaggt attgtcgtag attcaggttt aacatttggg aagttgcgtt
     3841 tttccgcatt acgccgagaa gtacggaagc aaaatgagga tggaacaatt ggtaatgagg
     3901 taaaagaacg aacctataat ttaaaatcct ctgcacaagg gcgaatgatt caagttagta
     3961 tcccagctaa tgtaccctta cgcgagtttg cttatgatgc agaggtagag ttggtcaatc
     4021 caatcgtgga taccgttgcc aattatgttt ttcgagaagg aactaccgtt aattggttca
     4081 ttaaggcgga tgatttagtc ttgaagcggc agccgaatca aggaaatcct acgaaccaaa
     4141 atgaaagaaa gaaataggtg aagattatgg aaattaatgt tgaacatatt ttagattgtc
     4201 tcgatcaata tggaaaaggc gagctgactg aggaacaact cgttaaagca ttaacctctg
     4261 atgaaaaaat gtttctgatt atacatcaag gtttacttgg agaaaatagc gacagtgaag
     4321 aagactctga tgtactagac tggttaggag aagaagagtc tttctttata gttgtcgaag
     4381 taaatgaaaa cttatgtcgg caggcagaat ccattttgga agaaattggt gtggagatgc
     4441 cggatgctat tgaaggcttt ttaaatcaat tggttgaaac gaaacaactc ccctcctcgt
     4501 agcagtaaat gactagcata actttttgaa attgcctaat gaaatcctat tgggcttttt
     4561 cgattggagg tggaaaccat caaaatgtac aaaggacatc gcatacgcgc aggagatcag
     4621 cacttggttt atcactttgt tcttggctgg ctactcgctc tgttcatcgg ttggatgagc
     4681 gtcttttatt ttcaagagtt tagacaattt gatatttcta agctatcact ttctactatt
     4741 gaaatcgttt ggtctataaa agatttagta tgtctgcttg gaagtctcgc tttttcagga
     4801 gctatgatat tgctttatat tcactttttt cttgatcatt ggcgaagctt gtggcatcga
     4861 caaaagctag ctcggatgat tctagagaac cattggtatg aagtgaaaca aactcaaagt
     4921 gaaggctttt tcaaagattt gaatagtagc cgaaccagag agactatcag ttacttcccc
     4981 aaaatctgtt atcgaatgaa agatggatta ctgtctattc gcgttcaaat ttcacttggg
     5041 aaataccaag atcagctttt aaaattggaa aagaagttag aaagcggact gtattgtgag
     5101 ttagtagaaa aggaactcaa agactcctat gtggagtata ctttactgta tgatatgatc
     5161 gccaatcgaa ttgggataga cgaagtagtg gcagaaaatg ggactctgcg attgatgaaa
     5221 aatcaagtat gggcttatga ttctttaccc catatgttaa tcgctggtgg cacgggtgga
     5281 gggaagacct atttcctcct caccattatc gaagcactat tgaagtcaga tgcagaattg
     5341 tttatcctcg atcccaaaaa tgcagattta gctgatttag gtacggtgat gcctcatgtt
     5401 tattcacaaa aggaagaaat ttctgcttgt gtggaagatt tctatgaacg tatgatggct
     5461 cgtagcaagg caatgaaaga aatgtctaac tacaaaacag gagagaatta tgcctatctt
     5521 ggacttccac ccaacttttt aatctttgat gaatacgtgg cttatatgga gatgttaacg
     5581 acgaaagaaa gtgcggtgat tttgaataag ctcaaacaaa tcgtgatgtt gggtcgtcaa
     5641 tctggtttct ttctgatttt ggcttgccaa aggccggatg cgaaatattt aggagacgga
     5701 atccgcgatc aattcaactt tcgcgtggct ttgggtcgta tgagtgaact cggatattcc
     5761 atgatgtttg gggaggtcga aaaaaacttc tttatgaagc acatcaaagg tcgaggttat
     5821 gtggatacag gtggcagtgt gattagtgag ttttatacgc cacttgtacc aaaagggtat
     5881 gatttcttag attcaattag taaattagaa aagatgaatg attgagtgat cttcaatact
     5941 gactttatac ttcaagtaac gtataatact tttatatagt aggaagaaag gggataatta
     6001 atatggaaag taatgattct tatttagagt atgcaaaagc aaataaggat aagtttattt
     6061 cttatattat caaagggaaa tcccccgaag ttggacgaga cgcagttttt atggcaggga
     6121 gtcctggggc tggaaaatca gaggttgcca ttggattagc tgagaattat gataaccatg
     6181 tgattattga tgcggattac tttcgcaccc agtttcctga ttataatgga aaaaactcta
     6241 gtctctttca aaaagcatct tcttggttgg tagagcaggc atttagatat gtcttagatc
     6301 atggctattc ttttattctg gatgcaactt ttgcaatatt aagtgctgaa aaaaatatct
     6361 cgcgggcgtt gaaaaaacat tttcgagtga cgattttcta tgtttatcaa gaccctaaaa
     6421 tcgcttggaa atttacacaa gaacgtgaaa ttgcagaggg acgtcatgtg ccgaaaaagg
     6481 catttattaa cgcttttttc aaatcaagag agaacattgc aaaagtaaag agtaaacatc
     6541 cagatgtatt gcttcatatc ataataaaag attatacgaa taatatttcc gaagttcatt
     6601 atgctacgga taatctagaa ttatttttac cactagggta tagtaggaaa gatttggagg
     6661 ttcaattaca tgactgagaa agaacaagta acgaaaattg taaagaaata caataagagc
     6721 attgcggatt tatcggaaaa tgctactgca aaagagttca aaacggtgat taaatatgta
     6781 gccgatcagg ccaatgaaaa acaacggaaa ttagttggat taaataaaaa atagtaggac
     6841 aaattaataa tgtatgagcc aagagcaaaa tgttcttggc ttttttcttt gcaatgaatg
     6901 aggtaatgta tgagattttt agatttattc gccggtattg gtggctttcg tttaggtatg
     6961 gaacaggcga gccatcattg tatcggtttt tgtgaaattg ataaatttgc tagacgtagt
     7021 tataaagcaa ttcatgatac aagtaaggag gtggaaatgc atgacatcac aagtgtatca
     7081 gatgagttta ttcaatccct cggaccagtg gacatccttt gcggcggatt tccgtgccaa
     7141 gctttttcaa ttgcgggaaa gcggcaaaga ttttccgata ctcgaggtac tttattcttt
     7201 gaaatcgctc ggttcaccac tcttctccga cctaagcttt tattccttga gaacgtccga
     7261 ggattgctca atcacgaagg aggggctacg ttcgagacga tcctccgaac gctggatgga
     7321 ttggggtatg acgtggagtg gtaggtgctt aactcaaagg cctacattcc ccaaagccgg
     7381 gagcggatat tccttatcgg acattctcga gacgcatgta ccgaacaagt atttcctatc
     7441 attggaccgt cttcaacatc tgatcaaaac atcagaaatt tggtaaatat caatccttcc
     7501 aatcgaggaa tgggtggaca agtctatggt tcagatggcg tagcgccaac cttaacaggt
     7561 gatgaaggaa tcaaaatcgc actgccagca agggatggaa tctctgtagc aggactatta
     7621 ccgggaaagt ttgaacaagg taatcgagtt tatgaagtta ctggaaccgc ccccaccttg
     7681 tcaacgaaac aaggtggtac aaaaatcatg attcgaaaaa atgaaacaga atatcaagag
     7741 gtgaagccag aagacagtgt gaatctggct tttcctaatt ccacaagtcg ccgaggtcgt
     7801 ctgggaaaac agagtgttca taccttacta acaggagacc aacaagcgat cgtcacggat
     7861 cagtatcaaa ttcggaagct aacacctaga gagtgttggc gacttcaggg ctttcccgat
     7921 tgggcttttg atcgagattc tcaagtcaat tcagatagtc agttgtataa gcaagccgga
     7981 aactctgtaa cagttccggt catttttgat attgccagaa gattaaaaga gttggatgta
     8041 catggtttat gatgagttga ttggagaaat ttattgggtg attggaaaga ttcagtcaga
     8101 cccagaatta gaagaacaat tgcgacggtt aaaatttgat attcgcaaga atggtgtcaa
     8161 agtcgctggc gatccatatt taatgaatga ggagacggat gctcgaattg aaataaatca
     8221 agtaattgca gaatttgagc ggattgcgga ccaagcaaaa gaaccagata ttcgtcaata
     8281 cttgttagaa atgaaagcag agttggaagt aaatggtatt acagatgagt aacctaaacc
     8341 aacacaactg actgttaggc gctgaccgcg gaggattcgc gaagcagaaa tcctccgtgg
     8401 tgctgtgcct gttcatggac ggcgttagcc gaccatgatg tctcaccccc gtatctaaca
     8461 ggggggtaca aaaacacaaa aaataagtaa gcaaccatta agaatgcttg ttgtatcaaa
     8521 gatagtaagt aatttgtata ggtgttaact catttgaaaa agttaacacc ttgaatttgg
     8581 aggtgagaac ttggcacaac ggaatttaga ttatcggcta ttaaaagacc gacgcaacga
     8641 atacggcgtt tcacaaaata aattggctac ggcttgtgga cttagtcggc cgtacttaaa
     8701 tcagatagaa aatggtggcg taaccgcatc tataaaaacc atgaggaaaa tttttgacca
     8761 actggaaagc tttaatcccg atttgccttt gacgttactg tttgactatg taaggattcg
     8821 ctttcccaca acggatgcac gaaaaattat tcaagaaatt ctccatttga aatttgagta
     8881 tatgctccat gaagattatg ccttttactc ctatcaagaa cagtatgtca tgggtgatat
     8941 tgtggtgatg ttgtctcatg aagaagataa aggtgtgctt ttagaattaa aggggcgagg
     9001 ttgccgacaa tttgaaactt ttttactcgc tcaaaagcgt agctggtacg actttttcga
     9061 ggattgtcta aaggccggtg gcgtgatgaa acgcttggac ttggcaatca atgatcgagt
     9121 ggggctattg gatatacctg agctaacgaa aaaatgtcag aaggaagaat gtatctcctt
     9181 gtttcgtacc tttaaaagtt atcgttccgg ggaactttta aaggctgatg aaaaggacgg
     9241 catgggcaat accttatata tcggcagtct caagagtgag gtttatttct gtttgtatga
     9301 aaaagactat gaacaatata tcaagttagg gattccatta gaccaaactg agacgaaaaa
     9361 tcgttttgag attcgactga aaaatgaccg tgcttttcat gcgattcaag atttgttaaa
     9421 aggtcgtagc attgagagca ccacgttttc cattatcaat cgttacttgc gatttgcgga
     9481 taaagtagaa ggtaaaaggc gaaccaactg gcccttgaat gaacaatggg ggcgttttat
     9541 tggacggaat cggaaagaga ttcaattaac ttccgagcca aaaccgtata ccattgaacg
     9601 aaccttgaat tggctaggcc gacaagtcgc ccctacgtgg aaaatggcta aggagttgga
     9661 tcgattgaat cggacaacct atatccagga tatggtacag aatgcacgct tatcagaccg
     9721 acataaaaag attttggagc aacaaagtat ggcaattgaa aatttgatta tatgaaaaga
     9781 ggaatcgtca tgggaattgt aaaagatatt tttttggtat ttggtggcag tatgcttggt
     9841 gttactgtga tgtgcttgat gaatgcaagt gcagcagcgg atcgagcaat ggaaaagaaa
     9901 ggaaagtgac aagatgaatt tcggacaaaa ttt
//
LOCUS       DAZULY010000105         9913 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000105 DAZULY010000000
VERSION     DAZULY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.106"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(379..603)
                     /locus_tag="ACG81A_001669"
     CDS             complement(379..603)
                     /locus_tag="ACG81A_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015030.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            794..3571
                     /locus_tag="ACG81A_001670"
     CDS             794..3571
                     /locus_tag="ACG81A_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5015031.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3652..4071
                     /locus_tag="ACG81A_001671"
     CDS             3652..4071
                     /locus_tag="ACG81A_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5015032.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4083..4553
                     /locus_tag="ACG81A_001672"
     CDS             4083..4553
                     /locus_tag="ACG81A_001672"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5015033.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4570..5349
                     /locus_tag="ACG81A_001673"
     CDS             4570..5349
                     /locus_tag="ACG81A_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5015034.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5336..6157
                     /locus_tag="ACG81A_001674"
     CDS             5336..6157
                     /locus_tag="ACG81A_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5015035.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6174..7223
                     /locus_tag="ACG81A_001675"
     CDS             6174..7223
                     /locus_tag="ACG81A_001675"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5015036.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7236..8240
                     /locus_tag="ACG81A_001676"
     CDS             7236..8240
                     /locus_tag="ACG81A_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5015037.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8873..9892
                     /locus_tag="ACG81A_001677"
     CDS             8873..9892
                     /locus_tag="ACG81A_001677"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS5015038.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3323 a         1644 c         2073 g         2873 t
ORIGIN      
        1 attcgtttat tgtgggtact atcttagagg aataaataat agtggagaaa actatggttt
       61 ggcgcagatt ctatcaggtt tgatggaacg aagacaacct cccgacatca agtcaaaagc
      121 tacttgagat tttgatctga aaataaaaaa agaaaacaag gtaggaacat caatgtttct
      181 accttgtttt cgtaatcaat gggaaaaaac taagagtggt gtacgtaatc aataaaaagg
      241 atggtgacaa cagtacctct ttactctctg aaagcatcat aactcgtttt tcctcattat
      301 gccaataaat tgttgttata ccacatataa ggatttgtaa tagctaaaaa cagacaggaa
      361 agttgaaatc tgtgaatctt attttgtatg gaaggattta tagaacgcaa aaatcaagat
      421 aaagatgaat aaaaatcctg tttcggctat atattgcgta tctacgccaa taaaacgagt
      481 ttgttctgtc atagccatat aaagcaaagg caaaaataca actacagcga gataagatcc
      541 gatgatttct cgaaaagttt tccaggaagt atgtttcctg tttttatatt tatccgattt
      601 cattgaatag tcatagcctc cttattcttt catatgttga aattccagta taaaaactgg
      661 aaggaactgt acgtttttta gaataaaaat tataagggat aagtaggctg ctgtctataa
      721 taagatatat ttttcaattc tttattaaag attttattga caaaatgaat ccgttttcta
      781 tgatggagga ggggtgaata agttgttgaa gaaggaaatt ttatgttact tagagaacca
      841 gacagccttt ttcgatttag aacatatgaa cgaggtcttt acagcacaaa ggttagctga
      901 tcattttggg gtaaaaagaa acacagtcag tcattatttg aaccagttaa atgatgagca
      961 actactagtc aaaatcaatt ctcgaccagt gatttatttc cacaaagaag cttttgaaaa
     1021 acaatttttt aagttacgca cgaattttta tttttctatc gatgatctca aagaggaaca
     1081 gccttttttc gcgcaaccaa aagacttatt ctcttttatg atcggatacg acgccagttt
     1141 gaaagaaagt atcgaacaaa tcaaaacagc tctttactat ccagatggcg gattaccact
     1201 tttgattaca ggcgaaagtg gcacaggtaa aagttattta gttaatttgg tctatcagta
     1261 ttgtctgctt catgatctgc tagaagattc tgcaccattc atcactttta attgtgcaca
     1321 atatgcagat aatcctgaac ttttgactag caatttgttt ggacatgtta agggagcgta
     1381 cactggtgca gaagaaaata aaaaaggggc ttttgaagca gcagatggtg ggatcttgtt
     1441 tttagacgag gtacatcgct taacaccaga aggtcaggaa aaactgttca cctatcttga
     1501 tcaaggtgtg atttatcgta tgggagaacc taatttgtca cgccggatca aaacacgtct
     1561 cttttttgct acgactgaag agattactag tcatttctta acaactttca ttcgaagaat
     1621 acctatccga atcgaactac ctgcacttaa tcagcgtagt cgtaatgaac ggctagaact
     1681 gatttactct ttttttctcg aagaacaaag aaaaatgggg agccctttgt cagtcagtgg
     1741 accagctttg tcacttttaa caagtcagaa attatccgga aacataggag agctgaaaaa
     1801 tatcgtcaaa gtttcagcag caaaagcata tgctgaacag cgtgaacagc ctgaaatcca
     1861 tgtcacgatc catcatttgc aaaaagaact acttgcgcag cctattacta aaaatacagc
     1921 acagcaagga atctatatta cgcaagacca gaccttagag caattaatgg agaagcaaca
     1981 gcccgaacag cagcgaatcg ttaaaagttt tgagcaaatc ctaattgatt tcaagaaaaa
     2041 taactgtctg ctatattctt gcgaaggaaa attaaagcaa gaagtgattc aattatttga
     2101 ttttttgctg tttgagacca gtagacaaga aaaacacgag ctgctggttt attggacaca
     2161 atctatccga gagacctttc gacaaatgga aactgcctat caaatcaaat tcgatggaaa
     2221 cagtgtctat gccttgagtt actatctcta tcagcgaagc actatcaaat ggttaccgga
     2281 agaacatgat attatccaac taatcaaaga attagaacaa caagtttctc agtcttatcc
     2341 atctagttat cactatgtcc aacgaattct ggaattaagt aagatcaaat tagatattga
     2401 agacacaagt atggatcgga ttttactgac gatctatcta aaaaaagcga aatggtcaaa
     2461 agaaaaacga ctgccaaaag cgatgattgc tgctcacggt tatgcaactg ctagcagtat
     2521 ggcaaatgta gtaaatcgtt tattacaaga ggatctattt gaatcatttg atatgccact
     2581 agatgtcaca ccacagcaga ttgcttcaga aatccttgat tattgcgaaa acagcgatgt
     2641 ttcaaatgga ttgatcatat tagtagacat gggatctctg aaagaaatcc atcagttttt
     2701 taaaaaacag ttatccgtac cgcttttgat tttgaataat gtgacgacac ctttagcaat
     2761 cacagtaggg gaatgcctgc aaaaaaatgt atcattagaa gagatagcag aagagactgt
     2821 tcgacagatc cagccagaat ggcgactgct ctatccagag gaaaacaaac ccaaagcact
     2881 cattactact tgcttcactg gaatcggtac ggcggtccac ttaagtgaac tgctagaaaa
     2941 aagcttacct acggcttgtc agttgaaaat catcccatat gaatatcagc aattaaaaga
     3001 taagaaaaac agtgacccat tattttccat atacgaagtc gtaggaatga ttggcaccac
     3061 agatcctgat atcaccaata tcgactatct ttcattagaa gacctcatct cgggggaaaa
     3121 gatgtctgta ctagctgaat ggttggacac tactatgaat gcttcagaga aggaaatatt
     3181 caatcagcgc atcattcgta atttttcatt ggaaaaagta ctggattccg tgactattct
     3241 agatacagag aaggtgatgg gagaaatcga tcattttatg agagaattag aggtgcagtt
     3301 gaatcgaagc atcagcaatc ccaaaaaatt agcgttgtat gtccatgtga gctgtctcat
     3361 tgaacgattg atccgacaaa tgccaatcga aacctatcaa ggattagaga aatggaagca
     3421 gtgtcaaaaa gaagggttat ctgctattaa atctgcgttt agtgtcattg aagagcatta
     3481 tagtgtcatg atccccaatt ctgagattgc ctatatctat gatattttga gtgaagatac
     3541 agagtttctc tcgatagaag aagaattttg aacataagca agttgttgcc actaattttg
     3601 gcatgcaact tgctttttta gtatgtgtaa gtgaaaagag gagatgaaaa agtggataga
     3661 gaaattatct tagcttccca tggaaaattt gcttcaggga tattagattc attagagctg
     3721 atctatggga aaaatcattc tattactgta ttcgattgct atactgatga gaattttgat
     3781 ttagcggaaa cagtaaggca gctttttatg aggtacaaag ataaagaaat cattgttctg
     3841 acggatcttt ttggcggaag tgtcaacaac gaattcttac aatatatcaa tcgggatcat
     3901 gtctatctcg ttgccggatt gaatttgcca ctattgattg agctagtatc tcggttggac
     3961 gctgaagtag agacaactgt tttgatccag caggtgctaa ctgattcaaa aaaaatgatc
     4021 cagttttgta acgagcggct caatcagaaa atagaagaag acgaatttta ggaggaagaa
     4081 aaatgattgt attgacaaga gtcgaccatc ggttattaca cggacaagta gctttttcat
     4141 ggacacaaac aataggcgca gactgtattt tgattgctaa tgatgatgta cctacgaatg
     4201 agatacgaaa gacaacgatc aaattagcca agccacaagg agtcaaacta gtgatcaaat
     4261 caattgatga ctcgattgcc gcgttgaaaa gcggggtgac agataaatat aagttattca
     4321 tcgtagtgga atcgatcgag gatgcatata agctggcaga agcgtatcca gaaatcaaaa
     4381 ccatcaactt aggagggatc aaggcaaaac ctggtactcg cagcatcgga aaagcggtaa
     4441 atattttacc agaagaggag gaacgcttga aacgattaat tgacaaacaa atagaagtag
     4501 aaatccgtca agtaccagca gataaaaaag tggatgtaag agaagttttg taagaaagga
     4561 agaacctcaa tgattgtaca agcagtctta ttaggaattg ttgcatttgt cgcacaatca
     4621 gaatatgcac tagggacatc gttgctttct cgtccgattg tgacggggct gtttacagga
     4681 atcgtgttag gtgatgtaaa aactgggatc attatgggcg caactttaga gttagcattt
     4741 atcggatcgt tctcagtagg tgcttcgatt ccacctgatg ttgtgaccgg tgggatttta
     4801 gggacggctt ttgctattac agctggagcc ggtacagaaa ccgcgctttt actaggtttg
     4861 ccgattgcta cgttgacatt gatattgaaa aatatttact tagggctttt gattccaatt
     4921 atgaaccata aagcagacaa ttatgcagaa gaaggaaatt ataaaggagt cgagcatatg
     4981 cacttgcttg ccggtatcgg tctttccttg atgcttggtg ttgtggtaat ggtttcttat
     5041 atggtgggaa gcaatacgat cggaaatcta ttaaatatga ttcctgactt cgttcaaaag
     5101 gggttgtcag tagctacagg gttgattcca gctcttggtt ttgcgatgct tgctcgtttg
     5161 ctgatcaaca aacaagttgc accatatttt ttcttaggtt ttgctatagc ttcttactta
     5221 gagattcctg tgaccggagt agctattttt ggtgcgattt tagcagttgt ggtagtcaat
     5281 attatgaatg tccgtcaact accacttcaa acaactgcag gggaggtaga agaagatgaa
     5341 gaattctgag atcaaaaaca gcaaaataac gaaaaaagag ctaaatcacg tattttggcg
     5401 ttcttttcaa atggagtttt cttggaatta tgaacgtcag atgaatatgg cttatgttta
     5461 tgcaatgatt ccgattttga aaaagttata tcagtcgaaa gaagaaatgg cagcagcttt
     5521 gaaacggcat ctagagtttt ttaacacgac cccacatatc gttacactga ttttaggaat
     5581 cacggctgca atggaggaag aaaataagga tgatcctgaa tttgatgtga ctgcgatcga
     5641 tagtatcaaa acatctttga tgggacctct tgcagggtta ggagattctt tcttttgggg
     5701 aactttgcga ttgattgcca caggtgtggg tacctcttta gccatgcaag gaaatatttt
     5761 aggccctatc ttgttcattt tgattttcaa tattccgcac attctgtttc gctatctagc
     5821 aacaggttgg ggatatcgct taggaacggg ttttttgaaa aagatccagg caaacggtat
     5881 gatggaaagt ttgacattag gcgcgtctat tattggactg atggtcgttg gagcaatgac
     5941 tgccacgatg atcaccatca atattccttt aaaaatcggt tcaggagaaa atgctgtgac
     6001 tgttcaaagt atttttgatg atatcgttcc aaatatctta tgtcttggag catttggcat
     6061 agtgttttat ctattgaaga aagaagtgaa accattaacg atcttgatcg gactagcttt
     6121 ctttggaatt tttggttcgt ggatagggat tttttaaaaa aggagcagag aaaatgttta
     6181 cgatgcaaga ttatatctat gaagaaaagg aagtactaag tacgatcttg aaaaagaatg
     6241 atttttcgac aagagaacat atgaaaaaaa cagtgaattt actgatctta gcaacgggtt
     6301 catcttataa cgcttgttta gcagcgaagc cagcattgga atcttatggt gatcttactg
     6361 tcgatattca agaacctttt tattttttca attatggaaa actttctcct tcaatcgata
     6421 ctgtgattgc tgtgtcacaa agcgggaaaa gtgcttctac tgttgaagca gtaaaaatga
     6481 tacaaaaaca agggttacct gttgttgcta tcacaaatga cgtgcaaagt ccacttgctt
     6541 tagaagctgc acaaattatt gatttaggtg ccggggtaga atcagtaggc ttcgttacaa
     6601 aaggctattc tgcgactgtt ttacagttac tactcttagg gataggaatt ggaataagta
     6661 aaaataaaat cagcaaaaaa atagaacaag actacatgca acagttgcgc aaaataatca
     6721 accatttacc agcgattatt caaaaaacgg aagaattttt tgatggatat caaagtttat
     6781 ttcgtttagc acagcgtttc gtcaccattg gatatggtcc aaactgggga acagctaaag
     6841 aagcagagac gaaattgact gaaacaatcc gcgttccgtc acaaggattt gagttagaag
     6901 cttatatgca cggcccatac ctggaggcag atgcttcgca tttgctattc tttattgaag
     6961 gggaaagtgt aaataaggaa cgttcgcaaa agttgcaaag atatatgagt cggtacgttg
     7021 gagagactct caccattacc acaaaaaaag caagaaacga gaaaacactt ggattagcaa
     7081 tagaatgtga tgaatatctg tctgtattag cgcttgtcgt tccgtttcaa ctattcgctt
     7141 ataaaaccgc agtagctaaa ggtattgact tgaacaaaaa gatttttgaa gactttgata
     7201 cagtcttgaa aagtaaatta taagaggtgg aaatcatgct gaaattcaat gaagaagaac
     7261 aaatcaaagc aataaaaggc gcactagctt tacgtccgca agtagaagaa atcatcgata
     7321 aactttatgc ggaaaaattt gacgctgtct attatctagg aatcggtgga acgtatgctt
     7381 cctcgatgca agctgtcact tatatgaatg ggaaaagcaa cttgcctgtc tttgtacagc
     7441 atgctgcaga atattacact actggaaaca aaagattaac aaaagattct atcgttattt
     7501 tgtcctctgt taccggtaca actcaagaag tagtgaaagc agtagaacaa atcaagaaag
     7561 taggagcgac actgattgga tttatcgata aagcgaacag taagttgtct cagctatgcg
     7621 attttgtggt cacttatcca gctcctggaa cagaacaaat caaatttttt atggcagcag
     7681 atcgattgat gtatttgaat ggagaattcg aagcttattc agaatactat gaacagctcg
     7741 aacgttattt accaacaggg ttagtggaag cagagaaaaa agcagatgca tttggcttat
     7801 catttgcaga aaaacatcga cgtgatagta tgcattattt tattggagca gggaatcaat
     7861 ggggagctgt ttattcatat gccatgtgtt actgggaaga acaaagctgg cttccatcga
     7921 aatctatcca tgctgccgag tttcttcacg ggacgttaga gatcgtagaa gaaacaaccc
     7981 cagtcacatt atttttaggc gaagatgaac agagagtgtt atctgaacgt gtggctaaac
     8041 ttttgccaaa aatttgcagc aattatacat tgatagacac aaaagactat ccagtagaag
     8101 gtatcagtga aaaatatcgc ggacgtgttt tgtctttctt attgatgcat gtagtgacac
     8161 aacgcatcga tgctcatgtg gagcagttga actgccatcc attagagata cgccgctact
     8221 atcgtcagtt tgattattag gatagactgg ataaaaagaa tataaaaaat aaagagagca
     8281 gcgataactg ctccctttat ttttttaaaa tattggcatc aattacgtta taaaacacat
     8341 tttttgcatt atctacagtt caacttataa tctgtttaat gggacatgtg ggtgccaacc
     8401 attttttgtt atgcaatagt actatttagt tgttgcatgg atccgtagta aaagtccaag
     8461 tacaacgagt tgcgccagtt tacatagcgt tttgtatcca gaatgttgac taaaacaaaa
     8521 gttcctatgt caaccgtcaa cagagacaat ctgtccgttg caggacctga cagatttaga
     8581 aatgttcaat ctgtctctta tgtattttaa atcaatactc actaggagaa gtttcctaac
     8641 ctcctaatag taatgctggt agtaacattg tcaaaactgc ttcctcttag gaacaagcaa
     8701 agcataacga gtgtaaaaat aaaatgtaga ctattgtaac atatcattga ttatttcaag
     8761 taaaaacgat aaaagaagta aacaaacact taaaaacaat ggaaattagt cccaacttta
     8821 agaaatgtat actataatgg aaattatcaa ttatttattt ggaggaatat ccatgaaaca
     8881 aaaaatcgca gttcttggtc ctggatcttg gggcactgct ttagcacaaa cattggcaga
     8941 aaacgggcat gatgtccgta tttggggaaa tgtacctgaa cagattgatg aaatcaataa
     9001 gtatcataca aatcagcatt ttttacctga tttgacgatc ccagaatcga ttatcggata
     9061 taaagaattg gcagaagcag tggataacgc agatgctatt ttatttgttg ttccaacaaa
     9121 agcgattcgt tcagtcgcac aggaacttat tggtaaaatg aatactaagc caatcattat
     9181 acatgcaagt aaaggactag aacaagatac gcataaacga atctcagaag ttttggctga
     9241 agaaattcca gaagaaaaaa gacaggcaat tgttgtatta tctggtccta gccatgctga
     9301 ggaagtggca gtacatgata tcacaacaat tacagctgca agtattgatc aaaaagcagc
     9361 agcctatgta caaaaactgt tcatgaatga ttatttcagg atttatacaa ataatgatgt
     9421 gattggcgta gaaactggtg cagcactgaa aaatatcatt gcaattggtg ccggcgctat
     9481 ccatggatta ggattcggag atgatgcaaa agcagcaatc atgactcgag gattggcaga
     9541 aatcagccgt cttggtgtcg cgatgggtgc caatcctctg acatttatcg gtttgagtgg
     9601 tgtgggggac ttgatcgtta cttgtacaag tgttcattct cgcaattggc gtgccggtaa
     9661 cttgttagga cagggacata agctggaaga agttctagaa aacatgggaa tggtcgttga
     9721 gggtgtggca acgacaaaag cagcagtaga gctagctcaa cagctaggtg ttgagatgcc
     9781 aattactcaa acaatctata atgttttgta taatggagaa gatatcaaaa aagcagcaaa
     9841 agaaatcatg ttacgtgacg gaaaaatgga gaatgaattt tctcttcgtt aaatagatga
     9901 tcaatagtta atc
//
LOCUS       DAZULY010000106         9893 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000106 DAZULY010000000
VERSION     DAZULY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.107"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            241..720
                     /locus_tag="ACG81A_001678"
     CDS             241..720
                     /locus_tag="ACG81A_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFS5015039.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            753..2981
                     /locus_tag="ACG81A_001679"
     CDS             753..2981
                     /locus_tag="ACG81A_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5015040.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3110..4753
                     /locus_tag="ACG81A_001680"
     CDS             3110..4753
                     /locus_tag="ACG81A_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS5015041.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4826..5371)
                     /locus_tag="ACG81A_001681"
     CDS             complement(4826..5371)
                     /locus_tag="ACG81A_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFS5015042.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5394..5879)
                     /locus_tag="ACG81A_001682"
     CDS             complement(5394..5879)
                     /locus_tag="ACG81A_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5015043.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5869..6681)
                     /locus_tag="ACG81A_001683"
     CDS             complement(5869..6681)
                     /locus_tag="ACG81A_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFS5015044.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6820..8352)
                     /locus_tag="ACG81A_001684"
     CDS             complement(6820..8352)
                     /locus_tag="ACG81A_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFS5015045.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8461..9492)
                     /locus_tag="ACG81A_001685"
     CDS             complement(8461..9492)
                     /locus_tag="ACG81A_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5015046.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3294 a         1839 c         1903 g         2857 t
ORIGIN      
        1 tggtgaagtt caccaacatc aaaaagtata gagccaaaat attccaacat catcttggac
       61 tttctacatg ttactaatga gcgtgtaaaa gaacgagatg gtgtttttat ttctatcaat
      121 gaagctcttt ttctttgcaa tcctcacttc aatcctctat aatgtcgcat atagtttagt
      181 aaatgagtaa actactaatt tacttattta ctaaaaataa aaggatgagg agttaaaata
      241 atggaaatac caacaaattt aaaacataaa ccagtaatcg ttgtagaaga ctatgataaa
      301 gttgatggaa gaaatgcatt aaatatggat gcaaaagggc tttcactggg ccttgctcag
      361 tggaatgatc gtggaaaagt cgatatctca ggcaaggtat ggcgacatac tggagaaaaa
      421 tggtcacgac agtcggaaga attaccaatc acacgtattt tagatttagc gatcttagct
      481 gcgcaaggaa gtttatactt ccaagatgct tatcgttatg aaaaatttta tgatccagaa
      541 aatccagtcg tagacatcat tggattacaa gggaaccgaa tgacggtaga agtagatacc
      601 aaaaatccaa agatcgatga agacatcatt ttgtattacg atacactaca aaaagacgga
      661 gaactgattg gggaacgatt ccgtatttta aaacgtcttt tagatgagct aggatattaa
      721 tagacagaga tgatcaaaag gggaagagaa gcatggagaa tttctcacta acagaaaacc
      781 gaaccttttc acggctagaa aagcaaatga tctggaagaa accgaaaagc catcggacaa
      841 gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa ttggcaagat cctgaaatga
      901 aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg gaagacacaa ttagccaaag
      961 cactatctgc tgatctgcaa cttccctata caaaagtgac ctgttttgca gatatggaca
     1021 aatcagatgt tttcggagcc ttattacctg tagtagattc taataaggaa caagaccaag
     1081 aattactaga agctatttac caaacagata cacttgaagc tgtactgaca ctgatccaga
     1141 accattacgg cattgatcct ttttctgcta aagaaaaatt agcagagcta gttcaaagga
     1201 tcgaagctga ttatccagat gcaatccatt acagctatta cccttctgaa atcgttcgag
     1261 cgattgaaaa aggctattta ttagagattc aagagccgac tgtcatccga gatgcctctg
     1321 ttcttgtggc acttaactca gcgctcgaac caaatgggat gttgaattta ccgacaggaa
     1381 tcattcgtcg ccatccagat tgcgtcattg tgatcactac aaatcgaaat taccaaggaa
     1441 atcgcccatt aaatgaatct ttaagagatc ggatgcaaca tgcggaaaaa atggatcttc
     1501 ctgccttaga agtcatggca gaacgtgcga tggcgaaaac tatgatccaa aaacaagaac
     1561 ttctcttaaa gatggcagag attatccgtt tgttggatga aactgcaaaa gctaacgcca
     1621 tcaaaggtgt agcaggaatg cgttcttatt tttattgggc aaatacctat aagcaaggac
     1681 aagatttact ccagtcgatt tatccaaaag ttttgtataa attatccact gattcagatg
     1741 aattggcaat tcttgaacaa gctttgtctg acagtggatt gctactggaa cttcaggaca
     1801 tattgagaaa agaaaaatgg ggtcatcctc aatctgagca tacaaaagaa agaaccatta
     1861 gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc tgaagaaatt ctgcgcttag
     1921 atgaaaagaa atcgttgtct gctgaagaac caaatgaggg agaagagaaa caagaagaaa
     1981 caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga acgcagtagc gatatcgaag
     2041 gaaaaaatca aaaagaacaa caaacaggag atgattctca agaaatgtct gcttctgaca
     2101 tggaagcaca tgataaggaa atcaagaagc aactgaataa agaagcgcga caaatcatga
     2161 aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg accagctttc caaggaaaaa
     2221 cgccggaagc acgtgagcta cacatgcaag taatgccaat agtagaatca cttagccggc
     2281 aaattttaga gttactggat aatgaacaaa cagagacgta ccaaaaagga aaatacgaag
     2341 gtcagcggtt caatgcaagc aaagtagcat atggtgattt gagaaatttt gacaaaaaga
     2401 acccgccaca tgagcaacct tctttagcgg tagctgtacg gatcgatgaa tctgggtcaa
     2461 tgatacggga tgatcggatc gaagcggcaa aaaaagcagc gattgccatt gccgaatttt
     2521 ctaagaaagt cgatatccca ttgttgattt acggagatac agcagaccgt tcgtcaagag
     2581 aaaagacttc tttgttttct tataaagaat ttgaagacgg atttcaatgg ttaaatgaaa
     2641 aattagtcac tatgaaacca aggcaaaaca atcgtgatgg tgctgcactt cgtttagcag
     2701 cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt gcttaatatt agtgacggtc
     2761 agccaaaagc attgccggat tatacaggca aaaaagcaaa agaagatatc cagtctgttt
     2821 tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc agctattggc caagacaaag
     2881 aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga tattactgac ttgaatgaat
     2941 ttcccaagca aatgattcaa ctgattgccc gctatctatg ataaaataaa gctcaacgaa
     3001 cgcacttagc agattcgttg agcttttcgt ttctagttac cggtaacaag gatacttcta
     3061 aaatagaatc ggttataatc aagagggacc gttaaaggag tgaaacgata tgatacaaga
     3121 acaaaaatgg tggaaagaag cagtaggcta tcaaatctat cctgctagtt tcaaagatag
     3181 caatggcgat ggtcaaggag acatcaatgg gatccgtgaa aaacttaatt atttgaaaga
     3241 tcttggtatc ggctttatct ggatcaatcc gatctatcaa tctccatttg tcgataatgg
     3301 ttatgacgta agtgactatc aagatataga agagagattc ggaacgatgg aagattttga
     3361 tttattgcta aaagaagcac atgaattagg gatcaaagtg attatggatc ttgtcatcaa
     3421 ccatagttcg gatcagcatc aatggtttga agaatcgaaa aaatctaaag aaagcccgta
     3481 tcgcgattat tacatttggg tcgacggggt agatggcaaa gaaccgaata attggacatc
     3541 gattttcggc ggttctgctt gggagtattc tcatgaaact ggacagtatt atctgcatgt
     3601 ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag aaattgaaag aagaactatt
     3661 caatatgatc cgatggtggt tggataaagg gatagatgga tttcgtttag atgcgatttc
     3721 tcatgtaaaa aaagatgaat attcagtaaa agccacagag aaccctttct caccctttca
     3781 aaatgtctca ggaatcgaag aacatttgac cgatttgaaa catgtttttg aagagtacga
     3841 tatcatgact gtcggagaag caagtggtgt cactgcagaa gaaggaccac aatgggtagg
     3901 gaaagacggt tatttcgata tgatattcga atttgatcat atccatatct ggcagcaaga
     3961 aaaagaaggg caattagatg tccttaaatt gaaacatgca ttaagcgctt ggcaaacttc
     4021 attggatggt atcggatgga acgctttata tatggaaaac catgatgttc ctcgtgccgt
     4081 ttcagtgttt ggagatactc gtcccgattt ttgggctatg tctgcaaaag caatcgctat
     4141 gatgtatttc tttttgcaag gtacgccatt tatttatcaa ggacaggaaa tcggaatgac
     4201 gaatatgccc tttgaatcga tcgatcaagt tgatgcagta gattcgaaaa gactttataa
     4261 aaggctgctt gcggaaggta agacaagaga agaagcattg gatatcattc gtgaaacaac
     4321 gagagacaac agcaggacac ccatgcaatg gacttcggaa caatatgctg gtttttctac
     4381 acatgaacct tggctgatca ctaatccaaa taccaaaacg atcaatgtag aacaacaaga
     4441 atatgaacca gagtcggtcc tgcaattcta taagaacatg atccgcatta gacaaaccaa
     4501 taaaggtctg atatacggtt cttataaaga atatcttcat gaacatccac aactttatgt
     4561 ctatgaaaga tatttagaag atgaacaata tcttattatg gtaaacttaa cagaatcact
     4621 tgctgactat gaacttccaa aagaagcaga tcagtcctgg acattactct tgtcaaattc
     4681 ctctagcgga gaatttgaag ctaaaggcat actagcacct tatgaagctc ggttatataa
     4741 aacaaacaag taaaacttcc tcgttaaagt tcattagttg gactttgcga ggaagttttt
     4801 cagattgctc tgtcaattac tcatttcaat aattcgctct aggaccaccg atatatttag
     4861 gacgagagcg aagggctgtc acgtgagctt gtccaagggt tttcaaacga ggtctgacag
     4921 gtacttggac atgcttgata tgcataccga ttgatgtgtc accgatatcg ataccggctt
     4981 gtccaactac gtgttccact tcgacaggat cattgaactg gtcaaaagca gcaacagaac
     5041 aagcacctcc agcatgtagt gcaggaacaa cagagacaat ttcgaaattt tttgcttctg
     5101 ccactgttcg ttctactact aatgctcggt tcaaatgttc acaaccttgg acagctagcg
     5161 caatttcttt aggatccaac aactctttta acgtacggat gatccattga ccaacttccg
     5221 cactggaatt ttttccaata tgtcctccaa cgacttcgct tgtgctgcat cctaaaacaa
     5281 aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc attaactatt tcagtcaatt
     5341 ggtcttttaa ttctttttct gacaacttca ttttctaaaa ccttcttttt caattaatat
     5401 tgcttccgtg cttgaatacg ttgaagactt gcagttatag gaaaggcaag tcccattcca
     5461 atcagatttt ggaagatgtt tcctggtata gaagccactc cagccggcca atcatataaa
     5521 atccaaccgg cgataccata gccgatgatc ataactaagc ttcctaataa aagccctaat
     5581 ccccacatac gagtagaaga tttgctgaaa gaaccagcaa catatccttg taacccgtgg
     5641 atcactaatg aaaaaactgc ccactgcgga tatcctgaaa tcaaatcgat caatagacca
     5701 ctggaagcac caacaactaa tccacccgtt ggaccataaa gaaaagcagc ggtatagatc
     5761 ccggcctcgc ataatgtcac aaatccattc gtagccggta cagggatcaa aattgttaat
     5821 gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg ttaattgctt agttttcatt
     5881 tctcattcct cctttttgaa taccgccaga ccagacactt ccggtatgat cgttcatcag
     5941 cacgccatta gaaatacaat gggcaacaaa ttttttactt tctgctactg ctttggtaaa
     6001 atctgcccct aacacaagat tcgctgttat tgcagaagaa aaggcacaac cggccccgtt
     6061 tactgttgta gtgtcgattt ttttatgaga aaatacctca taatgatcaa ttgaatagag
     6121 aagatccaca gcttcatctc cagccatcct tgtgccgcct ttgatcacaa tgttttttgt
     6181 tcctgaagca gtgagttctt tagcgagatg gatcatttct gttaacgaag agggaagagc
     6241 ttgaccagaa agtaaagcag cttctgccaa attaggcgta atcatcgtaa caagtggaaa
     6301 aagttcttcg atgagctgcg aaatatacaa ttgattgatc acagcttcag tttctttgaa
     6361 tgccaaaaca gggtctaaaa tgattgggat attttgtttt gtttttatga agtctcgaac
     6421 aaccatgatt gcttcacttg aattcaataa cccaattttg ataccatcta atgaagtcat
     6481 tttatcaatc gaaactaact gtcgtttaag caattccaaa gatatagatt ccagcataaa
     6541 tcgctcatta atatctagtg cccctataga agtcaaggcg cttagtccga atagaccaaa
     6601 attctcaaaa gtttttaaat ctgtttggat tcctccacca gcaaatggat cagaaccgcc
     6661 aattgtcaag attcttttca taacattttc ctccatgaat aagagtataa cgaaattttc
     6721 aaaaaagaaa aacagccaat tggcatattt ttatctcatc caatttaaaa aaacaaaaag
     6781 aaaagaaaga ccaaaatgga tctttctttc cttaaatagt taggactttt gtgattttaa
     6841 ttctttctcg atttgaagcc gttgcacttt cattgtagct gttcgcggga ttgcatcaaa
     6901 atcacggatg atagggacgt tcaagcgagg aagttcatgg acttgctccc accatcgatt
     6961 ccaatccatt tctttatcag gagctaaagc aataatcggt tgtggagaat gatttttatc
     7021 ccttacaatc actacttctg ccaaaaaatc taacgaatct aataaaaagt cttcaatagc
     7081 taatgtacta ttgatcgttt cgatcaaatc cacttgccga tctttcaaaa ataggtggcc
     7141 atgcttatcc ataaaaccgt aatctccgct atcccaccaa tcgccataaa cattttcctg
     7201 gaaccgtgca tcttccttgt aataagtcaa agcccgtcct ttagacagta attgtatatg
     7261 accatctgtg ttttcaggta ggacattccc aactgaatct gtaatccgag cgcttgtcat
     7321 gtcttccagt ccaacgccca tatctcttgc atcacttgtt ttcaaactct ctagtgtatg
     7381 agccttaaga atcattggtc cgcattcact ttggccatat acttgaagaa ataccgcatc
     7441 ctgtgccatc gacgttcgca aaaatgtctt cattgtctgg ttgtttatag catcaaaagt
     7501 ggagtgatag tatcggatac ccgaaaaagc ttctggatgc tccttagcag taaaacgcca
     7561 ctgaacaaag ttattcggat gggtctctag tgcaattggc cggtgagctt ccaacatatg
     7621 aacaactttt gaactttgtg cgtttgctaa aggcatcata ggaaatccca tggacatcaa
     7681 agaagagata ccaatattga atctggaatg cactggagag atgtgaaaag cgattaattt
     7741 cttttccgaa atttttgtga aaactgtttt ttgccatttc gtccgccatc ccatagaatg
     7801 agcagaatga caaataagtt tagggatacc agttgtacca gaagtatggg tcatataaga
     7861 aatttcattt ttgtctaaaa atgattgaga aacagacaaa gcaggttgtt ccattaaata
     7921 aagaatagga atctgttttg attttgttcc atttgaaatt tctctcactc gcttttttgt
     7981 ctcatcatca aataaaatga aagggtcttc taaacgatcg ataaatactt ccattgttgt
     8041 tgttggtaga tgataagaag tcatgacagg cactgcacca aggtaagaca cagaaacagc
     8101 tagaaggtat gtgtcaaaag cggaactctt ataaataata actttgtctc cggatttcac
     8161 tccaagatga gccaattgat aagcccgttt caacacactt tcgcagcatt ttttgtaagt
     8221 tgtttcaagt cctaattctg ggaaagctga aagtggctca tcaaagataa tcggggtctc
     8281 ggaaaagtta gctgcagctt cttgaaaatt cgtaaataag ttcaaagggg aatacgttat
     8341 ggtctccatc attcacactc cattatcttt tgttaagtaa tatacaaatt caagataatc
     8401 gtaacataat cctttatata ataacatagt ttattgtgaa aaaaagatca tacatttgtt
     8461 ttatatggaa gaaacccgaa cgaatgccgc taattcgtga taagaaaaat caaccatctt
     8521 ttcccagata tcttgaaaag aatgggatga gaggtatcga ttaccacata aataatttac
     8581 cgcgtcagca tcatctaaat atagttctgc ataataacga tatgtttttc tgcatagttc
     8641 tggcgatacg tttttatata ctgtaatttt gctcaaaatt tggcgaatag tccgaggaga
     8701 aatttgctga tcatgtgagg aacgaaaaac aaaagaattt tcgtccgtat tttgtgctgt
     8761 taacttaaaa tagttctcca gaaatggcat cgcttctttt ggtattggta ctttattcag
     8821 tctgttttct tttctattca gattaagaat ctgcttggtc aaagatacat ctttacgttt
     8881 aagttctact acttcatgga tctttaatcc gcaatacaga agaatggcag taattgcaag
     8941 gttcctcggt ctattctttt cttcaaagct agcttctctg cttgtttgaa agccttcaat
     9001 cgggataaac aaatgctcaa aatctttgaa aagttctgca tcgatatatc gcggagctgc
     9061 tttggttgtt tccggtaaac ttgtacccaa aagagaaatc tcttcttcaa tcagtccttt
     9121 ttgatacaaa aatcgtaaaa aatgatttaa tgaatattgc ttccttgaaa tcccgtaata
     9181 gctattttca aacaattgct ccgtttcttg gttcgctgta gtttggaacg aacgagtatg
     9241 tcgatagatt ttagctaaga aattctgata atcttgtttt tctaaatgga taatcagctg
     9301 atcatccact tcttcagaaa atacttttct caaataatca gtaaacagat gaaaatcttt
     9361 tgcgtattca tagcgactgc ctaatgaaag ggaagaacga ttttcttcta aaaactgttg
     9421 aaagacggaa gggagcaagt gtgtacgttg ctttaacttt tcttgcagtt gttttctagt
     9481 tttgctgtcc attacaaatt ccctccaata gtgttcattt tctatatttt accgcatttc
     9541 atcaaatgaa gatagacagt ttgtcttgat cttaggaagc aaatttttaa ctaagtacta
     9601 gctaaaagat atgaagtaag aagatacatt tttagttatt ttattagtgc agtcattctt
     9661 ttgatctttt aattgattta tcctcttttt agctatgtgg ttacttcatg agcaatgaaa
     9721 tggatttggt tcgttctaat ggcaggaggc tctttctttt atttccgaaa aattagaaaa
     9781 cattttttca gaatcgattg gctctccatt tattcaataa tggtttgtct aacgatattg
     9841 ttgggataat tcttaaagaa gtattagaaa tagttggtac ttcattactt tac
//
LOCUS       DAZULY010000107         9886 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000107 DAZULY010000000
VERSION     DAZULY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.108"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(47..559)
                     /locus_tag="ACG81A_001686"
     CDS             complement(47..559)
                     /locus_tag="ACG81A_001686"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFS5015047.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(573..2870)
                     /gene="recJ"
                     /locus_tag="ACG81A_001687"
     CDS             complement(573..2870)
                     /gene="recJ"
                     /locus_tag="ACG81A_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFS5015048.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3012..3455)
                     /locus_tag="ACG81A_001688"
     CDS             complement(3012..3455)
                     /locus_tag="ACG81A_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFS5015049.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3456..4244)
                     /locus_tag="ACG81A_001689"
     CDS             complement(3456..4244)
                     /locus_tag="ACG81A_001689"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5015050.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4241..5182)
                     /gene="rnz"
                     /locus_tag="ACG81A_001690"
     CDS             complement(4241..5182)
                     /gene="rnz"
                     /locus_tag="ACG81A_001690"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFS5015051.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5359..5946)
                     /locus_tag="ACG81A_001691"
     CDS             complement(5359..5946)
                     /locus_tag="ACG81A_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFS5015052.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6167..7480)
                     /gene="obgE"
                     /locus_tag="ACG81A_001692"
     CDS             complement(6167..7480)
                     /gene="obgE"
                     /locus_tag="ACG81A_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFS5015053.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7768..8778
                     /gene="gap"
                     /locus_tag="ACG81A_001693"
     CDS             7768..8778
                     /gene="gap"
                     /locus_tag="ACG81A_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS5015054.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(8863..9798)
                     /locus_tag="ACG81A_001694"
     CDS             complement(8863..9798)
                     /locus_tag="ACG81A_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5015055.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2719 a         2266 c         1707 g         3194 t
ORIGIN      
        1 aatcgcttct ttaagaataa catcaagaat agcttctttt ttctgcttaa tattccatta
       61 atgtaatgat gtcatatcct ttgattttgt cgcgtccatg gagatccatc aattcgatca
      121 aaaatgcgca gcctacgact acaccgccta attgttcgat cagttcgatc gtcgctttga
      181 ttgttcctcc agtagctagc aaatcgtcac aaatcagcac acgttgacct ggtttaatgg
      241 catccttatg caatgtcaat gtatctgttc catattctaa tccataagtc acttcgatgg
      301 tttctcgagg taacttccct tttttgcgga ctggggcaaa accgactcct aactcataag
      361 caacaggaca tcctacgatg aatccacggg cttctggccc aactaccata tcaattcgtt
      421 tttcttttgc ataatttacg atttgtttag tagcttcccg ataagcgtca ccatctgcca
      481 tcagtgggga aatatctcga aacacaattc cttttgacgg atagtcggga atactggcaa
      541 tataatcttt taaatccact ctttgtttcc tcctaggttg ttaaccattt ctttatggta
      601 ggaatatcgc tcaaaagcaa aaattcctca actttgattt tattgagccg tcgctggtat
      661 aatctgctct cagtcaaagg atgactttct gggctagcga ctttttgtaa aacaccgttc
      721 tctattgtaa caaatttcaa ttcaaaaaac acctgtatca taaaaattaa taatttttca
      781 gggatacgca aatattgagc tattgctttt attttatgac gtatatcaat tcgttcttga
      841 gaatggatca atttaaacaa ttttgcgtac tgttctcttg tgcctacccc attcaaatag
      901 gcctcatcag gtgaaatccc catcagataa acacgattga atgaataaga ccgaaaaact
      961 tctttcagat ccgctaaatc atctggacaa tccataacaa ctagagagtt tggttttctt
     1021 ttttcaatta ttgcctttac agaatctagt ccttcgaaaa caatcgcagc ttcctggata
     1081 atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta aataaaggct accttccgac
     1141 gttcggctct gttcacggaa acgttttgcc cgccaatcaa atacttgcag accacccaca
     1201 gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt tccattcatt gatcgaaagc
     1261 tttcctacca catctacctg attattcagc agttcttctt cttgagtacc aaaaccaaaa
     1321 gcgaccgcat caagctgact cgatgcatcg gaaaggacaa atttcagatg ctgttggttt
     1381 gctccgattc ttttgatatt ttctgcttgg acttgtcgaa ataaaaactg aggcaaagga
     1441 ttatccgtac caaatggcgc caatagtttc agctcatcaa ttcgctctac tgttacttca
     1501 tttggcagca atacttcatc aattctcaac tcaggccctc tcatcaagtc aatttgatga
     1561 tcgactatat attggttcat cttctcctgt agtatagtga cattttcact tggcatcgtt
     1621 aaaccgactg ctgcatgatg tcctccgaag taagtaaata aatctcgcat ctggtcaagc
     1681 atttcaaaaa gattcaaggc ttcaatacta cgtccagatc ctttagctga tccatcttct
     1741 tttaaagtaa ggacaagcgt tggtttaccc gtttcattca ttattttccc agcaacaatg
     1801 cccaaaacgc cttcatgcca tcctcggttt accaatagat ggatatgatt ctccttattg
     1861 accatcgcta atgcttcttg tgttatctgc tctacgattg cttttctttc ttcattgatc
     1921 tcattcaatt tttttgcttg aacggatgct tcctcttcat caaacgtcgc aagtaacgag
     1981 acggcaggac gtggatcgcc catccgaccg attgcattta gtctgggagc aatcgaaaaa
     2041 ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc tttcttcaaa tagcgcctga
     2101 agtcctatcc gttctgaatg atgaatggca tcgattccta atgaaacaag gattcgattc
     2161 tcatcagtca atgaaactaa atcggcgatc gttcctatcg ctactaaatc caaaaattct
     2221 gcaggcggtt cttccaataa tgcagaagct actttgaatg ctactccgac tcctgccaaa
     2281 tctggaaatg gatagtgccc cgctggatga cgagggtgaa caatcgcata agcgtctggt
     2341 aaattctcag gtagttcgtg atgatctgta atgatcacgt ctacaccagc ttgctgagcg
     2401 tatgctaccg catcatttcc tgctaccccg ttatcaacag tgatgatcaa ctggattcct
     2461 tcttcgatct tttcttggta taccgattgg tttggtccat acccatgctc aaatcgattt
     2521 ggcaggaata cttctacttc tgcaccaagt aattccagtg tttctttcat taccgttgca
     2581 ctggtgatcc catctgcgtc ataatcaccg taaacgagga ttttttctcc actgattaca
     2641 gcttcttgga ttcggctgat tgctttatcc atatcgtgta tcagatacgg atcatgtaat
     2701 tcttgttcct ttggatgtaa aaatcggttg atatcctttt cctcatggta acctctttgc
     2761 cacagtaact taccgacaaa tggagataat ttcagttcac ggatcatttg gagaaaggat
     2821 tcagaaggct ctgtctcgct taacttccaa tcaaaatttg cttgtctcac tttctcactc
     2881 ctaacttagt tattatagca aaggaaatca gcaaaaaaaa gcagaaagaa agaataaaaa
     2941 cagatcagat ccgctagaca gatgttcagc aggacctgat cttttgtacg accatttatg
     3001 aaatatccct attagtcaaa ataattgaag ctttgtttgg gcgattgagt tttttgttct
     3061 tccggcgctt tcaattgact gatttgtgcg gttttcgctt gtaactccgc ctgataagca
     3121 gcttccaact ccgcacgttc gtttctaaac tcgcgttgct gttcttcttt tgcttcttca
     3181 attttctttt gaagttctgt ttgatattct gacatttcct gagtcagctg tttgatttgc
     3241 tttttttgct gccatagtgt tgccgaagca gtcaaaaata caatcagtgc accaatcagc
     3301 gcagaaccga tgatcactaa gatcaatggc gcagaaaagg aagtgaagcc aaaacttaca
     3361 ggaacatttt tattgttcaa tacagcaaaa agaacaataa tcaaaactaa aacaaatcca
     3421 agaatcacat tgctttgctt tttcaattcc ttcacctact ttttgttaaa aattccgctg
     3481 gctaaataat caccaatcgt tggaaacagc gtataaaatt ttgatgcaac ttctaaaata
     3541 aacggttgat tgatttcccg ttttggatgt ctcatattgc ggacgatcat ttttgccaac
     3601 ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat agcttccgct tggatcagcc
     3661 ttatcgaaga aatttgtctc gattggacct ggattcactg tcgtaacagc tacgccaagt
     3721 ggtttcaatt ctaatcgcaa agcgttggag aagccaagga ctgcaaattt cgtggcagaa
     3781 taaaccgttg attttgcagt tgccattttt ccagccatag aggcgatatt aatgatatga
     3841 ccttgtcttc tttctgccat atcgatcgca aatttttgtg tcaaaaccat cattcctaag
     3901 atgttgactt caaacatatc gtatgctttt ccaaggtcga atgtaaggaa gtcttcgaaa
     3961 atcccaaaac ccgcattgtt taccagaata tcgacgcgcc cgacttcttc catcaccttt
     4021 tcatataatt cgtccacgct ttcaggattg ctgacgtcta ccttgaaagc ataagcctct
     4081 ttgccgctca agcgtctgca ctcgtctctg acaccttcta caaatgccat tcttcgtgcg
     4141 caacttataa cgattgctcc ttgctttgct gcttcataac agatttgtgc gcccagaccg
     4201 cctgaactac ctgttactaa aacaacttta tcttctaaat tcatgcctct tcctcctcgc
     4261 gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt cgtgttttca aacacttctt
     4321 gtgcttcttt ttcaagctct aaagctgctt ttcctaaata tctcgcactg atatgagtaa
     4381 gtagcagccg ttttgcctgc gcttcttttg caacttcagc agcttgatga gtcgtggaat
     4441 ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa tgtgctttca tgtaccagaa
     4501 tatcgctatt ttccgcaagc acaacggaat tatgtgtttt tctcgtatcg cctaaaatag
     4561 tcacgattcg tcctttttta tctggaccta cgaaatcttt tccattgatc gtccgcccat
     4621 cttctagttg gatcgtttca ccttgcttca gtttgccgta cagtggtcca gctggaatac
     4681 ctaattcttt tagacgatcc acctgcaatt ctcctttatg atcatgttca accacacgat
     4741 aaccaaaact gtcgatccca tgatttagtt tttttgtata aacagaaaat tgctgatcag
     4801 taaaaatcgg ggctgtttca tttagctcaa tgaatttcag cggataactt aaacgcgttt
     4861 gcgaaatacc taaagagact tttatgtatt cctctatccc ttttggcccg taaatctcta
     4921 atggcgtatc cccaccttga aatgaccggc tgctgatgag cccgggaagt ccaaaaatat
     4981 gatcgccgtg taaatgtgta atgaaaattt taccgatttt tctcggacga atagtcgttc
     5041 gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa ccaaacttca ttgcgctcgt
     5101 ctaaaagttt caatgctata cttgtcacat ttctatgttt tgcaggaaca cctgcacctg
     5161 ttcctaaaaa ttgtaattcc ataatctacc tgactttctt taagttcctc tttaatttta
     5221 gggtaaaaag aagggataat caaccgtaag tcgagaatgg gttcaaatta aaaataacgt
     5281 tctaaagcga tagcgttttc tttgaaaatt actataaaaa cagcagcgaa cccgtttgag
     5341 atcctctgct gttttttatt aatttgaaga tccagaactt tcattgtcgt tggaagtttg
     5401 cggatgtttg tccttccaag tcttttgtgc agcttctttc gcatgatttt cagcttcttt
     5461 cgcatcatgc acatcttttt cgacatcttc ataatccaag cgagtaattt ctccgcctaa
     5521 ttccatcatt accaattgat tcagtgggcg attgtcctct tcatcagcaa tcaagatcag
     5581 tcctgtagct ccttcaccga ttttatttcc tacaaagtcg aacacggtct gggcatcttt
     5641 tatttctttg gcatcttgtg tcgcaccgat cattcctccg gcaaaccatc ctaataaaat
     5701 ccctagcgga ccacctaata caccaaccaa cattccgatt aatccacccg tagaagattt
     5761 gttattaccg gtaaagtcga taaaatcttc tattttgaat tgatgtgtcc cattaggatc
     5821 atggctgaca actgccatct gttctccttt aatcgaacga acagcatgta actttttgat
     5881 ttctgaaaat gcctgatatg cctggctttc tacttgaaaa tttaaaatga ttactcgttt
     5941 gctcattaaa aacgcccctt tcataccttc ttgttattta ttttaccttt aattgaaaaa
     6001 taagaaaaga aaagcactct ttttttccaa taaaataggg ctgtgacaaa agtcgtatca
     6061 cagctccttt ttccgaataa acggcgttct tgaagctgat cacttctgtc acaacctccc
     6121 ttaaatctga atctatattt tttggtattt gtttttattt gcttgcctat tcgacaaatt
     6181 caaattcaaa ctcaccgata cgtacaaggt cgccatcttt cgcaccgcgt gcccgaagag
     6241 cttcatccac acccatacca cgtaattgac gagcaaaacg catcactgtt tcatcatgat
     6301 cgaaattcgt catttggaat aatttttcga tcttctcacc agataagatc cacgttgcat
     6361 ctgaatcacg atcgatttgg aactcaggac cttctggatt gaatccatag tgtacagttt
     6421 cttcttccag ttcttcttcg tacaatggga attctggcgt tacttctaac aaatctgctg
     6481 tagcattcaa taacgcgtcc aatccttgtc gagtcactcc agaaattgga aatactggga
     6541 tgtcatcagc aaattcatct tctttttcct tgttcagctg ttctttgaat ttcactagat
     6601 tttccggtgc atccggcata tccattttgt ttgccacgat gatttgcgga cgctctagca
     6661 agcgtaagtt ataagtagat aattctttgt tgatggcaag atagtcttca taaggatctc
     6721 gtccttccat cccactcata tcgatcacat gtaaaattac tcgtgttctt tcgatatgac
     6781 gaaggaattg tgtacccagc ccaacacctt gcgaagctcc ttcaatcaag cctggcaagt
     6841 ctgctgcggc aaaacttctg ccatcacttg tagtaaccat tcctagatta ggtacgagcg
     6901 tcgtgaaatg ataagcgccg atttttggac gagcagatga aataacggat agtaaagtcg
     6961 atttcccgac agatggaaaa cctacaagtc caacgtctgc caatactttc aattctagtt
     7021 cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat ttccggtgca ggattccgag
     7081 gagaggcaaa acggatattc cctcgtccac cgcgtccacc ttttgccaca actagtgttt
     7141 gaccattttc aattaaatct cctaaaagag caccagtctc cgcatctcgg acagtggttc
     7201 cttgtggtac tttaacatag gtatcttctg aaccacgacc gtgcatccct ttactcatcc
     7261 cattttcacc tggctgcgct ttgaaatggc gattgaaccg aaaatccatc aatgtacgta
     7321 acccttcatc gacgatcaag atgacatcgc cgccacgacc accgtctccg ccagccggtc
     7381 cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat tccgtctccg cccttgccag
     7441 ctttcacatc gatcgttact tgatctaaaa acatggacat aattaagtcc tcctattatt
     7501 catttattta tttgactgcc tgaatttcca caataaacaa aagtcttact tattttatcg
     7561 gtgaaatctg attttgtcta tcatttcagc aaaaagattg tttaccttct tcttttttta
     7621 tcatttggta aggtttccaa ccttttttct gctaccaagt ataatttttt gacaaaatat
     7681 tctttgcgta gcagaataac agtgtattaa ttgtattaat tgaggatgcc gtttcacagc
     7741 acccctaata aaggaggaac ttttgatatg acagtaaaag taggtattaa cggatttggg
     7801 cgtattggcc gtctggcttt ccgccgcatc aaagaagtct cagatgatat tgaagtagta
     7861 gcaatcaacg acttaacaag tccaactatg ttagcacaat tattgcaatt tgactctact
     7921 catggtactt atcctggcac agttactgca acagaagact caatcgtagt ggatggcgaa
     7981 gcaactcgtg tttatgcaga acccgacgca agcaaaatcc cttgggcaaa agaaaacggt
     8041 gtagatatcg tactagaatg tactggtttt tatacatctg aagaaaaagc aaaagcgcat
     8101 ttagatgctg gtgtcaaacg agtagttatt tctgccccag ctggtgctat gaaaaccatc
     8161 gtctataacg tcaatgatga tacattagat gaaaacgaca aaatcatctc agcaggttca
     8221 tgtacaacaa actgtttagc acctatggct tatttcttaa ataatgaatt tgggatcgaa
     8281 gttggtacaa tgacaacagt tcatgcctac acttccacac aaatgctctt agatggacct
     8341 gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg acaatactat cccacattct
     8401 actggtgctg cgaaagcaat cggcttagtt atccctgagc tacaaggaaa attacaagga
     8461 cacgcacaac gtgttccagt cgttgatggt tcattaactg aattggtttc agtattgaaa
     8521 acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa aacacacagt tgacaatcct
     8581 tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg tcattggaac aactcaaggt
     8641 tcgatctttg acccaacgca aacagaagta acaactgctg gtgatttcca acttgttaaa
     8701 actgttgcat ggtatgacaa tgagtatgga tttacttgcc aaatgattcg cttactagaa
     8761 aaattcgcta acttgtaatt tgatagatca atcgataaaa gaagcctttg actggatcca
     8821 gtcaaaggct tctttctttt catcccctac ttttaaactc gttcaatgaa catattggct
     8881 taaaaattgt tggactactt gctgatattt ttcaggatct gtcttgtatg cttccgcatg
     8941 ctcggctcct tttacaacat acttttcttt tggtcccttc gttgcacgat agaccttatc
     9001 taacatttca aatggtacaa atgtatcttc gtctccatgt ataaagagca ttgggcgctg
     9061 gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa aagtagccag cacggacttt
     9121 tgtcactaga ctcgttactt gcataagcgg aaatgctgga agactaaaca tatcgtctag
     9181 ctgataagca agctcatcat gaacggaaga ataaccgcaa tcttcaatga ttgctttcac
     9241 attatctggt agtttttctc cgctagtcat cataactgtt gctgctccca tgctgacacc
     9301 gtatagaacg atttcttgcg attttccatt gttttctagc actttgttta tccactgtac
     9361 atagtcttta cgctctggcc atccaaatcc aatgtaatcc ccttcgcttt ttccatgtcc
     9421 tcttgcatca ggaacaagga cattgtaacc catatcatga aacatctttg cgtaattggt
     9481 catcgtttca gcgtttccca tatagccatg agccacaaga gctgtttttt cacttttcgt
     9541 ttcagcagga agataaatcg ctgataattt cagaccgtct tttgaagtga tggtccaatc
     9601 gcttcggttt tcttttgaac taaaccattt atgtgcatcc gtttctttgg atgtctgtgt
     9661 tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa ggcacaacag cataattata
     9721 aaaataattc cctgcaaaca ctaacgcaac aatcagagct aaagcgacgc ctaaaaggat
     9781 tttcatccaa agtttcataa atcatccatc tcatttctct tttttcatat aaccttgaat
     9841 tagtataatc aaagaatctc tactatgcaa tgttcatttt tataac
//
LOCUS       DAZULY010000108         9877 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000108 DAZULY010000000
VERSION     DAZULY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.109"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..422)
                     /locus_tag="ACG81A_001695"
     CDS             complement(60..422)
                     /locus_tag="ACG81A_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFS5015056.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(611..793)
                     /locus_tag="ACG81A_001696"
     CDS             complement(611..793)
                     /locus_tag="ACG81A_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015057.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            768..2207
                     /locus_tag="ACG81A_001697"
     CDS             768..2207
                     /locus_tag="ACG81A_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5015058.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(2214..4766)
                     /locus_tag="ACG81A_001698"
     CDS             complement(2214..4766)
                     /locus_tag="ACG81A_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFS5015059.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(4880..6211)
                     /locus_tag="ACG81A_001699"
     CDS             complement(4880..6211)
                     /locus_tag="ACG81A_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5015060.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(6177..7655)
                     /locus_tag="ACG81A_001700"
     CDS             complement(6177..7655)
                     /locus_tag="ACG81A_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5015061.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(7960..9795)
                     /gene="tdc"
                     /locus_tag="ACG81A_001701"
     CDS             complement(7960..9795)
                     /gene="tdc"
                     /locus_tag="ACG81A_001701"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFS5015062.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT         2988 a         2107 c         1637 g         3145 t
ORIGIN      
        1 ttcttatttc ttgaagctaa acatttctgt cacaacctct tctctattgt cctagacatt
       61 cattgcgttg atttaaaact tccatcaatc aaatctgcta gttcatctag cttttctatt
      121 ttacgtagat caatagagat ccaatgttct ttattcatgt gataggctgg aaaatacccc
      181 tcttttcctc gcaaaattcc gccaagttca ggatcgacct tcaaatcaag aatattgatg
      241 atcctatggt cacttgcatc atcatagatt ttttcttctg gaacagacat caggacccca
      301 aaccattttt tgcttttttg atgtctgaat acgcaataat caggaaattt tttaaaaata
      361 tattctggag acaaattgta tgtttcattg atgtgatcga ttagttcttt tttcgttagc
      421 atctgcttcc atctctttct tattgattcg ctttattcaa agataccacg taagaataga
      481 aaaatgaata caagtagttg ctaatcaaat acttcgatat ttttcttttt tatcaactct
      541 taaatgttca atccgcctgt gttggtcttg ctaccatcta ctattaaaat gtttattatt
      601 gtttgcccac ctattttttg attttagtgg tatcattttt caagttcatg atgaaattct
      661 tatcatcagt aaatgctcca gaaaaataaa caatgattga aaaatcagtt gaaacttgac
      721 caccgtttat tttctccgta ttcttagaat agaagggagt ctgattgatg tattcactaa
      781 tgaaaaaaat cattaccgaa aaagatattc gacgtcatgt ttctttagta gaacaattgc
      841 tgaatcatac aaagataacc gtaaatgaat tagctgaaat catagggaca accgaacgaa
      901 cgattttttc tgaccttcaa tctattcgct ctcagctgcc cgaaggctgg gacatcatca
      961 gcgatcaagc aggaatcagt ttgcaaaacc agcaaaattt attaacaaat gacctttggg
     1021 agattttttt caaacaatca gtcagtgtcg aactgctgaa aaatctgctt tttacaaaaa
     1081 aagttgctgt ccctgatttt ctagccgacc atgggctttc ttatgggaca ttgaaaagac
     1141 atgtgactaa aatcaatcag cgtttagcaa gttacgactt gcagatcgat ttgacgaaat
     1201 atacagcttg tattcttgga aaagagcgtg cgattcgtac tttttatcat cgattgctga
     1261 ttcctttcac tcataacaac tattttttcg aggattattc gatccatgaa tcccattatt
     1321 ttcaattttt aagaaacctc agtcaaactg aactagcggt tgaaacagaa gagattttcg
     1381 gaacatgttg gttctttatc aatacgatcc gtataaaagc gaattgccga ttagattctt
     1441 ctatccatac caacagcaca ctctcctccc tgtatgactc tgcattgaaa aaattgtatt
     1501 taacggaagg tatttatctt aaagacactg agctttcttt tgcttctttc tgctttctgg
     1561 aaagttggaa ttacaacaat aactatggac aagaaaccgc tcgctgcctg catcattctc
     1621 catttttaga ggtcctcgaa acgtttgtag aggaactagc ttcagagctg tctcttgatc
     1681 aactaaaaaa aacatctctg actgataact tggctctcct catattaaaa tatcatgaat
     1741 cgccgatatt gattgaagca ttggaccgtc aataccatcg ttttttagaa acatatgatc
     1801 agcaatatgc acacttgtat caacagaaag agaatttact gaacgatcta aaaaaagaaa
     1861 tagaaatcaa tgaaccaaat tactttctcc aactcttgtc tctactgatc caacaaacga
     1921 ttttttctat caaaccgaat ttgttcaatg tctattttat tttccaagga gaaccttcat
     1981 ggaaggcgtt cttgcagcag gaattaaaag attacttagg aaaacgtgtc tgcttcttac
     2041 ctatcgaact aaccgatctt tccactattt cttttgaaaa aacagatatc ttgatttcca
     2101 atttccctct agattctcta gatatcccta ttgtatacat ttcttctatt ccaacgaaaa
     2161 atgagctcaa tcgtttgact gaactcactt tcaaaacttt tttatagtaa cggctagcga
     2221 ctgtattctc tagctgtttt ttcttttttg cgaaacagtt tgttcatgat ccctttttct
     2281 atttctacaa ctaagaatag aagaatcaca tttgctccaa tcaataactg ttgtgtcatc
     2341 gttaaactta ctgtaccgat cagctgctgg ccgactggta gaaaaataac catcccttgc
     2401 aataaagcaa ggatacctaa tgaaagaaaa agcgctttgt tttgcaaaag accttggttc
     2461 aatgacggct tggacaactc tctgcaattg atcatgtaaa cagcttgagc taagacgata
     2521 ttttgcagaa gaatggtttg ctgtaagctt tgcccttcaa actgcatagc caataaatag
     2581 gcaggaacca tgatcagtag ggagacatag atgatccgaa acaaatgata accagatagg
     2641 attccttgct ttggatctct tggtgggcgc ttcatgattt ctggatctgg cgactcgaac
     2701 cctagtgcat aagataaagt gattgtcgtt accatattga cccataaaat ctgtactggg
     2761 gacaaaggta gcgggcgatt cagcataagt gcccaaatca agatcaatcc ttgagctaat
     2821 gcagttggca agaagaaatt gatggttttt tgcaaattat ccaaaatccg ccggccctct
     2881 tttacggctt ttgcaatcgt atggaaatta tcatctccta aaaccatatc agctgcctgt
     2941 ttgcttactt cactaccttt gattcccata gcaattccta catcggatct tttcaaagca
     3001 ggtgcatcat ttacgccgtc tccggtcatt ccgacgattt ctccattttt ctgcaaagct
     3061 ttaacaattc gcagtttatg ttctggtgtc gttcgagcaa agacatcgac tattggcact
     3121 tgctgaatca actcttcatc agacataaga tccaaatcga tcccttctaa aactttggca
     3181 gtatgtttta agccaatttg ttccgcaatc gcttttgctg tgtctttgtg atcccctgta
     3241 atcattttca ctgagatccc tgcttgttgg cattccttta cagctttgac tgcactttct
     3301 tttggcggat cgatcaatcc ggcgatccct gcaaaagtta attgggttaa agtctcatgc
     3361 gtgagttcct gtttcgaatc tactttttta taagcaaatc ctaaaacacg ctgacctttt
     3421 ctcgcaaatt ctgcagctgt ctgactccat gcttcttgtt ctgcagaaga aagagttgat
     3481 ttagcaaata atacttctgg tgcacctttg acgtaaatca ccgctccttt ttcctccgca
     3541 tgcatcgttg ccatatattt gtattcagaa ctgaacggaa tctttgcttc tatttttttc
     3601 aaaggtagat tggctgtttc tgtgtgtcga atcagcgcaa cttctgtcgg attgccactt
     3661 aaattttctg ttttctgttc ttcttttgtt tttaattcct gacagttttt cataatggac
     3721 aacacgcagg cttcttcttt tgcagctaca tccacaactg tcatttcatt ttttgtcaat
     3781 gttcctgtct tatctgaaca gatcactgtc atagaaccta atgtttctac cgaaggaagt
     3841 cctttgatga tcgccttttc ttttgccatt tcatgtactc caacggatag aatcatggtc
     3901 aaaacagctg gcaatccttc gggtaccatt gacaccacca aagcaatcgt agccgaaaga
     3961 aggatatgcc aatccatacc atatcgaaat gtcgtaaaaa taactaagaa taggctcaaa
     4021 caaagaatgc cttgaaaaat ttgtttgttt aatcggttga tttttttaat cagaggcgtc
     4081 gtttgttgtt cgaccgattg caatgcttga ttgattttac cgatttctgt cgtgtttcct
     4141 gtttctacaa cgacaccgac tgcagaacca gattgtacaa gcgttcctga aaaagccata
     4201 tttttctggt cgccgatcga ttcttcttct gtcaaaacgg catgactttt ttcagatgct
     4261 tctgattctc ccgtcaaaat cgattcttcc actaataaat catgtgcttc gattaatcgg
     4321 atatctgctg gaacgacatc tccggcacgt aagacaatga catctccttt taccagttct
     4381 ttcgcaggga ttttttcttg catcccgttt gatagtacta tcgcttcttg tcccatcagc
     4441 tgcttcaaac cattcaatga ttcttccgct tttctttctt gccagtaact aacgagtcca
     4501 ttgacgatca ctacgaataa aataatactt ccttcaatat attcactaga agcaaatttc
     4561 aaaaaagagg caatgatcaa cacgatcatc agcagatcag taaaatgctt tgctattttt
     4621 ttccatcgtt ttgtctgctg ccttgctttt atttcattat atccttgatc agtcaggcgt
     4681 ttctgtcgtt cttcttgcga caagccttct gttgatgttt gatagttttt caatacatct
     4741 tctaccgtca atctgtacca gttcatccta tcccctcctg tttaattgat actgttatta
     4801 tacgcaggga agttttttca ggcgtatcga ttatttcagg ggggtctgaa ataattcgtt
     4861 ttgcaaaatg tatctgggcc taactgatat ttctattgct tattctttta tatacaaaat
     4921 ttaagaatac aaaccagata accaataaaa aaggaataaa gcgtgtcgtg ctttcgaata
     4981 acaagctgaa agcaattgaa gtaaaaaaca ccaacaaagc aagatcttta tatctttgtg
     5041 tagtgttccg tttcttttct ttaataaatt gaacatgtgc acacaagatc gaagaccaca
     5101 caaatagaaa acaactcgtt gtcatgcctg ccaaaagctg aaaaaacaat cgactatcag
     5161 ctacaaaaaa atgtagacaa agaccggcaa aaaataacat tcctgtaatt aaaataccgt
     5221 gtaaaggtac gcctttctta gaaagcgcac aaaaccaatg gggcaaactt ttttcttttg
     5281 ctgtttgata taaaatcctg ctagtactgt agactgcact atttgctgag gacaaagaag
     5341 caaccaaaat cattagattg acaattgctg ctccgccttg gcaatggatc acttgcagca
     5401 tagtcactaa aggactttct tttaaatcta aacaatgcca tggaacaaca aataaaatcg
     5461 acaaaatcgt tcctatatag aaaaagagaa tacgccaagg cagacagttg attgcttttt
     5521 tcaaattctt ttgcggatca cttgtctcgc caacggttaa gccgatcatt tcaattccag
     5581 caaaactgaa taaaaccata ggaaatgaga ctaccattcc agcaaagcca aacggtagta
     5641 attcatcgat ctgtccaaaa ttttctttga ttccgataga ctgggtatga aaatctgaga
     5701 cactcaatcc tatacccaat aagataaaaa ggaaaatccc caagatcttg atgattgaga
     5761 aaaaagattc caattcacca aaccacttga ctgcaacaag gttgactgcc attaagatca
     5821 ttagtgcgct acctccgctt atcaaagctg gtacatgtac aaaccagaaa tggatataca
     5881 atcctattgc tgttaattcc gcaagtccag ctccaagcca gcaaagccaa taagtccaat
     5941 tgacaaaaaa tgcccacttt ttgcctaaat aatagtcaac gaaatcactg atagagttaa
     6001 atgtactatt tgctaaaaat aaatcaccca tggcccgcat caaaccataa atagccaaac
     6061 cgcatatcaa ataaacgaga ataacagctg gccctgctga tgcaattgcc tttgctgaac
     6121 ctaaaaataa tcctgttcca attgcgccac ctaatgcgat taactggata tttcgattag
     6181 ttagagtccg tttcattgcc ggtttttcca tcaagttcac ctttttcttt ttcaagcagc
     6241 atggcgactt cctgttccca agaaggtttc tttatttttt caatacagag tggtactaca
     6301 aacaaaatga cactgatggc tgtgactgca attagattga tccatgttcc agctttcatc
     6361 gtcagtccga cagaaaatcc gattgccgca aataaaacaa taacgacagt ccaaactcca
     6421 agattgctct ttttctcttt ttttccaatt ctaaatggtc gattcaactt tgcatgtgta
     6481 taacgcaatc tcaaaactgc gatccccatg atgacataga cgatacagta gataacagta
     6541 gttgaattta cgagcatcaa aaatgcttga ttgacgccag gaatcagcaa ataaaaaagt
     6601 gcaaagattg agataatgac agactgagtc aaaataacgg atttggatac gtcaaattga
     6661 ttcgtcttcc aaaaattgaa ttttggtgga tacgcacctt ttctagcact ttctgtcact
     6721 gtctttgctg gtcctgaggc ccaagcagat aattggactg ctacaccaat gaagacaagt
     6781 aaactaaaca gattcacaat aagatgtggc aaaccaagaa tttggcaata aatcgaaata
     6841 gattgcgcga tattattcaa ttccatttgt ccttttggta cgacatttgc tacaactaaa
     6901 gcattcaaaa tattgacgaa aaatataaaa attaacgcgg caaatactcc gcgaatatac
     6961 gttttaactg gattttccaa tcgagtgata tacacagagg acatttcgat accagtaaaa
     7021 ataaacatga tcggcgcaaa ataaacaaat gaggatgcag tcgtagtatc tggtattaat
     7081 ttgtcccatg aaaaactgcc taatgttccg gttggtacga tcccaacttt gatccacgca
     7141 gcaaacccta ataagagcat catcgtcaaa ggcacgtaca atcctaacca aacaccgact
     7201 tttccgccga tttttgccat atcaaatttc aaattcaaaa gagtgatgat ccaataaacg
     7261 actaaaatga caactaatgt aaatttgctg ttcaaaccaa gcggcacatt atcgataata
     7321 ttaccaaaca acggagcgag tgctgaagcg accatcacca tcccaggaaa catttggacc
     7381 cataacagcc atgagacaac gaacccccat ttttttccta gagcattcga tacccaaagt
     7441 tctggaccac ctttttgggg aaacattcca gcaaattcac cagagatcaa tgagattgga
     7501 aaagcgtaca ataatactgc aaccagcatg taaaaaatca tcgtccagct cgttgctgct
     7561 acatctggaa tatttcgtac acttgctaca agggcgcagg tcattccgat aaaggtcatg
     7621 gtcgtgattt gctttgatga accagtatct ttcatcttta ttccttcttt caatagcgac
     7681 atatgttttt gttttctttt atttttgttc aagaacagaa atgacaaggt ttcatcgatt
     7741 cagtacaaaa cccagtatca ccgaaacatc cattgaacga tcttagtgaa ctgggtttga
     7801 tacatgaaac gaacgatcca acttatagaa cgttttataa aaaagaatat cttggcgagg
     7861 aatatatgaa aaacaggggg aaatacaatt gttttgatga agcaaataat tgtctgttct
     7921 ccatatgtct cttgttattc tttcacacaa aaatcagttc tattggatga tttcgtttag
     7981 tttcttttcc attgctttgg cgattttttt tgtataaaaa ttgaaaattc gatcatcatt
     8041 caaataagga gtcatgatag ccgcacgtag cagagtgatt ttctgttctt ttttccattc
     8101 ttctttcgtt agacccattc tttcaacaaa acgaatcgga gaatcaccgt aatcttcttg
     8161 tgtaaatgta gtatgagaag taataaaccg ttcaccataa acatcgccat ccatataaga
     8221 agaccgatcg aacatttttt catttaattc attgatagcg ttcaaatctg tacagccttg
     8281 ttctttgaat acccaattga ccatattaaa atcaggatga ttcaatggat agacttcgat
     8341 tgtttttcct ttaacagtaa aagtcagatg atcgagaaat tctctgaatc tttgagctgc
     8401 ctcgattgaa gccccaatca attgaccata tccggtcaca ttcaatggca atacacgatg
     8461 tgctgtccag actgcggcgg cggttgctcc agcttttgat ccttccaaaa tataagctcc
     8521 taacatatct ggagccttta ctgatttttc aaacacatat ggcgcgaaat aagaaatgat
     8581 atttctcata tttttatgtt tgattacgat tcctccagca gcataaggga catagcccat
     8641 cttatgagga tctatcgtga cagaatccgc ttctgaaata gctctgaacc cttcatatac
     8701 ctctttgtca atcgttacgc agtggtggaa aacatgatgt tcttcaaaga attcagctaa
     8761 ggaagcatac ggcacaaatt ctcctgcttc gtttagaaac agtgaacgcg catagccgcc
     8821 atatgcggca tcaacatgta aatagaaata gatcccttca tcttttaacc tttctcttaa
     8881 ttggacgatt ttatctacac tgtctacttg accttcttct gtcgttccta cgacagcaac
     8941 cacacctaga attgggattt tttgtccagc taattcacga attgtttttt ccaaagcatt
     9001 aatatccatt cgataatcct cttgaactgg gatagcaacc atctgatcta agccgactcc
     9061 gcaaatatct aacgccttca tccaagaata gtgttttgtt tgagggacca gccatttccc
     9121 taatctttgg atattttttc cgctacggga agaagctgct ttgacctcat ccatttcttc
     9181 gtccgtaaag ctttcggtca tttccaaaat ttcttcaaca gataaattca aaagttccca
     9241 ctcagacatt tttttcactt tttcaggcag cacttccttg acagctaaag gaatcgattt
     9301 gatgcaacgg gcgtaccata atccttcaag attagcaata gaaccatctg ctgcaatgtg
     9361 tccccatccg tctgtcatat tgaataatga agcaaaatcc tgtcctactt ctgcttccat
     9421 ttgcgaagtt gccatagaag attccaatgc aacattattt gggttccaca gcatcgcata
     9481 gttgtaagct aataaagcag gcatcaatgt ttcagcattc atttgtcccc agtaacggcc
     9541 tgctgaatgc catgggatcg atcctgcgcg taatcgctga ctgacttcat ccaagacctc
     9601 tatcatatgg tcgcgggtgg ctagataatc tggagtgagc ttatctcctt cgctaattgc
     9661 aggcatatct gaaggaatat aattttctct ccatccaaga tgttcatcta ccatcttgtt
     9721 caacaacatt ttataaactg gtccattttc agctttgtcg cctataaaga cggccttgat
     9781 atccatatct ttcatttttc ttccctccat attttgttgg tcaatgtctt gcagatacat
     9841 gtgtttatct gtaacacctc ttcactatag cgccaaa
//
LOCUS       DAZULY010000109         9799 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000109 DAZULY010000000
VERSION     DAZULY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.110"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..306)
                     /locus_tag="ACG81A_001702"
     CDS             complement(28..306)
                     /locus_tag="ACG81A_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015063.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            574..2583
                     /gene="metG"
                     /locus_tag="ACG81A_001703"
     CDS             574..2583
                     /gene="metG"
                     /locus_tag="ACG81A_001703"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFS5015064.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(3062..3649)
                     /locus_tag="ACG81A_001704"
     CDS             complement(3062..3649)
                     /locus_tag="ACG81A_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015065.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            3824..5146
                     /locus_tag="ACG81A_001705"
     CDS             3824..5146
                     /locus_tag="ACG81A_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5015066.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            5301..6077
                     /locus_tag="ACG81A_001706"
     CDS             5301..6077
                     /locus_tag="ACG81A_001706"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS5015067.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            6070..6639
                     /gene="rnmV"
                     /locus_tag="ACG81A_001707"
     CDS             6070..6639
                     /gene="rnmV"
                     /locus_tag="ACG81A_001707"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFS5015068.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            6632..7516
                     /gene="rsmA"
                     /locus_tag="ACG81A_001708"
     CDS             6632..7516
                     /gene="rsmA"
                     /locus_tag="ACG81A_001708"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFS5015069.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            7967..8596
                     /locus_tag="ACG81A_001709"
     CDS             7967..8596
                     /locus_tag="ACG81A_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015070.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(8720..>9799)
                     /locus_tag="ACG81A_001710"
     CDS             complement(8720..>9799)
                     /locus_tag="ACG81A_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5015071.1"
                     /translation="KKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGKSTTLRMIAGL
                     EDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGLKLRKYDKADI
                     KKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMDEPLSNLDAKL
                     RVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIGTPKEVYDTPV
                     NMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGYEGKEVIFGIR
                     PEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKVDARDFHKPGE
                     IVDLAFNISKGHFFDKETEQVIKLP"
BASE COUNT         3213 a         1724 c         2011 g         2851 t
ORIGIN      
        1 atttacttga aaaatctagg ttatcattta aatatgaaaa ataaatggaa gtatgagatt
       61 gaataaaaag aaaagcagat aaaacggcaa tactttatac cgctcttcac gaatggcaaa
      121 gaagccacaa ataacgatat atacgtcaat gataaaacgc aaaatccaag ctgctgtcat
      181 aaaactgttc aaatgcccat ggaggttaaa ataaccatac atacgaatat aaaaatagct
      241 gaatattaaa tgtgctaatg aaagaaaagc aatactaagt gtaaaaaaaa gatgtttctt
      301 ctgcatgatt gttccagtcc cttactaaga tagatagtct ttattatacc acgtgtgagg
      361 ggagaaataa aaagccgttt tttagtagat atgaaacaaa acaaaaaccc gcatttattc
      421 aacctattca tccttttaaa ggatagattg aacgtagata gaagtcgttt atccagtctt
      481 tttcattttg aaaaaaagct aacagcatgc tttttttatg gtatactagc aaggaaacga
      541 attcgcatca tgcgataaag gaggaagagc cccgtggcag aaaaggaaac tttttatatc
      601 actacgccaa tctattatcc aagcggcaaa cttcatatcg gcaactcgta tactacgatt
      661 gcatgtgacg caattgctcg ttataaacgg ttgatgggct ttgatgtgtt ttatctcact
      721 ggagtggatg aacatggtca aaaaatcgaa acaaaagcag aagaactagg tgtaaaaccg
      781 caagaatatg tagataaaat ggcggctgat gtcaagaaat tatggaaaac gcttgatatc
      841 agttatgata aattcattcg cacgacagat gattaccata aagcagctgt tcaaaaaata
      901 tttgatcgtt tattagaaca gggagatatt tacttaggtg aatatgaagg ctggtactct
      961 gtttctgacg aagaattctt tacagaaaca caacttgcag aagtgtacaa agatgacgaa
     1021 ggtaacgtga tcggtggtaa agcgccaagt ggacacgaag tagaattggt aaaagaagaa
     1081 tcatacttct tccgcatgag caaatacgca gaccgtctat tggaatacta tgaagaacat
     1141 cccgaattca tccagccaga atctcgtaaa aacgaaatga tcaacaactt tatcaaacca
     1201 ggattagagg atcttgcagt ttcacgaaca acttttactt ggggagtacc agtcaagagt
     1261 gatccgaaac atgtggtgta tgtatggatc gatgcattat ccaattatat tactgcacta
     1321 ggatatggct ccgaagatga aagtctattc gaaaaatact ggcctgcaga tgttcagatg
     1381 gtcggtaaag aaatcgttcg ctttcatacg atctattggc caatcatgct gatggccctt
     1441 gatttgcctc tacctaaaaa agtatttggg catggctggc tattgatgaa agacggaaaa
     1501 atgtctaaat caaaaggaaa tgtcgtttat cctgaaatgc ttgtggaacg ttatggttta
     1561 gatgcactcc gttattattt gcttcgtgct gttccgtttg ggtcagatgg tgtctttacg
     1621 ccagaagatt ttgtttcccg tgtcaattat gatctggcaa atgatctagg gaacttattg
     1681 aatcgaacga ttgctatgat caacaaatat tgtgatggac tcgttcctga ctatgcctca
     1741 cttgtcactc catttgatag tgaactgtct acgacggctg ctaacgttgt cggacgttac
     1801 catgatgcaa tggaaaaaat ggaattcaat acagcattgg ctgaaatctg ggtgctgatt
     1861 tctcgtgcaa acaaatacat tgatgaaaca gaaccatggg tattagcaaa agacgaagaa
     1921 aagaaagctg aattgaatag tgtcatgatc catttagctg aatctttgcg tatcgcagcg
     1981 atattacttc agccaatcat gacagaaacg ccagagaaga tcttcaatca actaggattg
     2041 gactctgaaa caatgaatct tgaaggatta cactttggcg aatttccttc aggaacaaaa
     2101 gtagttgcaa aaggtacacc aatcttccca cgtttagata tggaagaaga agttgctttt
     2161 attcaagaaa aaatgtctga aggtactcaa acaaacgaag atacagtcaa atgggatcca
     2221 gaagaaacag aacttgtttc aacgaaagaa aaacaaatca aatttgatgt atttgaaaaa
     2281 gtagaactaa aagtagcaga agtcatcaac tgtcaaaaag tagaaggggc agataagttg
     2341 ctgcaattcc gactagatgc tggagacagc caagaccgtc aaatcttgtc aggaatcgct
     2401 gaattttatc ctgatccaag cgaattgatc gggaaaaaag tcgtcattgt agcaaatctg
     2461 aaaccacgaa agatgcgtgg acaaatcagt caaggaatga tcctttctgc agaagcacca
     2521 gatggctctt tacaagtaat cgaagcacca aaatccatgc caaatggctc agaaatcgca
     2581 tagaaattca aagggggtat gacactagtt gtgtcatacc ccctttttcc gaaatttgag
     2641 aagcaatttt cgtaaattcc ttcttattac ttgaagccaa tcatataata cagaaacaga
     2701 gaactatcca aaataaatcc tagaaaaaga agtacgagct tcgctgattt ttgttaattt
     2761 agaataatac taataggaat cgtaaaaaca gaaaaaaagc ataatgataa agggtcgggc
     2821 agattttaat tgattcatct ggtactcttt tttattgtcc ttacattcat aaagtttgaa
     2881 tgcaatgact ttattacatc tatatttata tctaacagta gcgttgatgt gttaattata
     2941 agttctatca ctttatgttc aattagtttg tgctcttata tgtgtgattg aaaatataga
     3001 taaaaaaaca atcatcggct ttgacttcga tgattgtttt ctttattttg taaaaatcct
     3061 atcacttgat tttatggtaa ccttttattt ttgtcttcaa acggcttggt aagagagcag
     3121 taagcagtgc aaatgggatc gaaaagacat accagaaaat cgtacgtccg ataccataat
     3181 gttgatagag ctttgtcaac atcacgtgta acaagatcaa ccctaaaagc aaggagccag
     3241 ctataaacca atgattatcc caatgcatga cttcactaag tgccatcact ccaaaaggat
     3301 agaaataaaa atagatcccg actaaggtca tccgccagtt ctttgtaaat gttccgatat
     3361 tcaataaaaa gaaaatcgct gaaagcaaaa taccgatagg cggaaaaagc ccaaccaaaa
     3421 aggaatacaa catcccccac aagatcatca caggaccttt gatcaatgca acgattgcga
     3481 tgaacaaagt cagcaatatt agtttttgtg ataacgttgc aacagtcatt aatccaaata
     3541 aaattaatgt gaaccaatgc caatgggact gctgttgttc tttacgggaa aaatcgaccc
     3601 ggttggataa ataatctgct aatttcggat aagaacgctt aaaatccata aactgttcct
     3661 ccttcgtgga aaaggtaggt ttagtatacg aaaaataaaa aagaaataca tccgtcttaa
     3721 gagggagttt ttcactgaaa gtggagaaaa aagaatggta aattaaaata ttctcagtta
     3781 tactgaaaat tataccaaat ttcaaagaag aaagaggatg ggaatgaaag gcaaactaaa
     3841 acgagaaatc aatctgtttg gcgcattagc taccgttatg ggaaccgtga tcggtgcagg
     3901 cgtcttcttc aaaactgctg ctgttactgc tagtactcag tctacaagtt tgacattgct
     3961 agcttggctt ttgggtggtt tcttaacgat ctgtgctgga ttgacagtgg ctgaattagc
     4021 tacagcaatc ccggaaactg gtggagctgt aaaatatatt gaagctgcgt atggaaaatt
     4081 gcccagcttt ttattaggat gggcgcaaag cttgatttac tttcctgcta atattgctgc
     4141 attatcgatt atctttgcga cacaattgac gaacttatta caattatcta ccgattatct
     4201 tttgttgatt gcagtgatca ccgctgtttc tgtgacaggg ttgaatttgt tagggacaaa
     4261 agtcggtgct tctgtacaat cagtgacgtt gattgttaaa ttaataccaa ttgctgtgat
     4321 tgtcatatgg ggattattga ccccaggaca aggaactgtc cagttatttc caatcgaagc
     4381 tggaaaagat gttacttttg tcgaaggatt gagcagtgcg ttgttagcaa cgttgtttgc
     4441 ttatgacggc tggttaggtg tcggtgcaat ggcaggggaa atgaaacggc cggaaaaaga
     4501 tttgcctaaa gcaattattc ttggtttgag ttttgtaaca gtcgtttatc tattgatcaa
     4561 ttttgttttt ttgaaaactt tgccgattga tcatcttgcc ggtaatttga atgcagcttc
     4621 tgaagcatca gacgtcattt ttggaggaat cggtggaaag ctggtcacta ttgggatctt
     4681 gatctcggta tacggtgcat tgaacggtta tacactaact gggatccgcg tgccttatgc
     4741 gatggcttta gaagacgagc tacctttcag taaacagctg acaaagcttt caaagaagtt
     4801 tacggttcct tatgttcctg cggttttcca gttagcagta gcttgtatta tgatgagcct
     4861 tggttctttc gactttttaa cagacatgtt gatttttgtg atgtggctat tcagtttact
     4921 gatttttatc ggtgtcttta ttttaagaaa aaaagcgccg gaattgccac gtccatataa
     4981 agtaccactt tatccaatcg ttccgattat tgcgattctc ggtgctattt tcattttagg
     5041 tatgacgatg atgacgcaaa cgaaattagc attgatcggt ataggtgtta ccttgatagg
     5101 tatcccagtc tattatcaaa aaaagaaaaa acgctcagaa gagtaaaata tttcaacaac
     5161 agacagaaaa agtacgaact aggtcctcta gatcttcgtg ctttttctat ttcaataaaa
     5221 caagttcttc tattttgatt tttgctactt ctgcaagtag tgcttatgtg ctaaagtaag
     5281 aatgaaaagg agagataaac atgatatttg attctcatac ccatttaaat gctgaacagt
     5341 tcaatgaaga tattccagaa acgattcagc gagcacaaga aatgggggta acgaaaatgg
     5401 cggtcgtggg cttcgatacg ccaacgattg aaaagtcttt gcaattaagc catgactatc
     5461 caaatatata cagtattatc ggctggcatc cgacagaggc tggcagttat acgaaagaaa
     5521 tcgaaaataa attacaagaa caattgactt taccaaaggt tgtagcatta ggggaaattg
     5581 gcttggatta tcattggatg gaagacccga aagaagtcca agataaagta tttcgcagac
     5641 agattgctat cgcaaaagaa atgaacctac caatcagtat tcatactaga gaagcacttg
     5701 aagacacgta tcaaatccta aaagaagaag atgttcgaga tatcggtgga atcatgcata
     5761 gtttcagtgg tgattatgaa tgggcaaaac gatttcttga cttagggatg catatttcat
     5821 ttagcggtgt cgtcactttc aaaaaagcat tagacgtcca agaggctgca gcaaatgtac
     5881 caatcgaacg attacttgtg gaaacagatg ccccttatct ggcaccagtt ccttttagag
     5941 gaaagcgaaa tgaaccaggc tatactcgtt atacagtaga aaagattgct gaacttcgga
     6001 atcttccgac agaagaagtg gctgcccaaa catggacaaa tgcgcaccga ttatttggtc
     6061 taagcgaaca tggataagat gaagatccaa gagattatag tcgttgaagg aaaagacgat
     6121 acaagaaggc tgcgggaagt agttgatgca gatacgatag aaacaatagg gtctgcaatc
     6181 aatgaagaga tcctgatgca aattgaacat gcccaagaga ctcgtggagt gatcgttttt
     6241 accgatcctg atttttcagg agaaaaaata agaaaaatca ttatggaagc tgttcctcag
     6301 gcaaaacatg cttttttgcc gcgcacacaa gctaaaccaa agaaaaaggg aagccttggt
     6361 gtcgagcatg caagtgatgc tgctatttta gaagcattga aaaaggtcgt gacgccagac
     6421 atttcccaaa cggctgtttc tgaaatcact agacaagacc ttttagacta tggtttaata
     6481 gctggggcac aagcaaaaga aaaacgagaa aaactaggtg acgagttgcg catcggttac
     6541 acgaacggca aacaattgga aaaacgtttg aagatgtttc gtattacgcc gaaagaactc
     6601 gcccaagcaa tgaaaatagt ggaggaacaa catgagtgaa tatcgtgaaa ttgcgacacc
     6661 ctctagaaca aaagagattt tattgaaaca tggtttttca tttaaaaaaa gtttaggaca
     6721 aaattttctg actgaaccaa atattttaag gaaaatcgta gaaactgcgc aaatagatga
     6781 acatacgaac gtgatcgaag ttggaccagg aatcggtgca ttgacggaac aattagccaa
     6841 gcatgcaaaa caagtcgtag catttgagat cgatgatcgt ttgatccctg tattggctga
     6901 tacgatgcaa ccctatgaca atgtgactat tgtacatcaa gatattttaa agaccgattt
     6961 gtcaacagct gttagagaaa catttcatga ggagcttccg ttaaaagtcg tagcgaattt
     7021 accttactat attacgacac cgatcatgat gcattttctt gaatcagatt tggttgtgga
     7081 tgaactagtc gtcatgatgc aaaaagaagt agctgatcgt atctctgctg aaccaggaac
     7141 gaaagcttat ggttcattat cgattgctgt tcaatattat atggaagcca gtttagcatt
     7201 catcgtacca aaaacagtat ttgtccctca gccaaatgta gattcagcga ttttgaaact
     7261 gacaagaaga gatataccgg cagtcgaagt gacagacgaa aaagcatttt tccgattgac
     7321 aaaagcggca tttcagcaaa gaagaaaaac gctttggaat aacttgcagc attcgtatgg
     7381 caaagatgac cagacaaaag catggttagc aaaaagcttg gaaacagctg gaatcgatcc
     7441 aaaacgtcgt ggggagactt tgtcattaca agaattcgcc gcattaagca atgcgatgtc
     7501 agaaaatatg cagtaaaaaa agaggttgtg acagaagttg tgaaaaagct gtcctgcatc
     7561 aagtaataag aacagccaaa caaaaatagt tcctcatgtt tttcgtttgt ttgggcttgt
     7621 taccgcagat gcaagctttt gaacaccgtt taagaaagag gttgtgggcc tgccgtttaa
     7681 ctctaagtca caaggaacaa tttcaaaaaa cagcttctca tgtttttgag ttagttggtc
     7741 ttattacttg atgcaagctt ttgaccacta ttatgccgaa caaggagatg tgacacaaat
     7801 tttgtcacat ctcctatttt tgtttcttga tcaaaaaatt cttatacttg atactcacac
     7861 tcattttgta tggatttaga aaagaatatg tgtcttgaca gttattagta agtgagttat
     7921 actaagtcga catttatgtg tcaagacata ttaggagaga gaaaaaatga gaaaaaattc
     7981 agttcgaaaa ctaacgattt ctgctttatt gattgggatg ggggtcatta tcccaatggt
     8041 catgcctaaa atcatgattc caccagcttc ctttactttg gcgtctcatg tgccattatt
     8101 tattgcgatg ttttttacac caggagttgc agtggctgtt gcgctaggta cgacatttgg
     8161 tttcttttta acaacgccgg ttatcatcgc attgcgagca ttgtcacatc ttgtgtttgc
     8221 gttgattggg gcttggtact tgcaaaagca tccgagtatc gttttaaaaa atggacagtt
     8281 tacttttgct aattggcgtt tccaagggtt caattttgtg atcggtgtga ttcattcttt
     8341 agcagaaatg ctagtagtca gcatctttta ctttattggt aatatgcctg aaacatatta
     8401 ttctcaagga tatttttata cagtattcat cttgatgggg attggtgggc ttatccacag
     8461 tttagttgat tataatattg cttattttat agcaggaaca ctcagcaaat attttgatat
     8521 ccccgtattt acggcagcaa aaaaagaaaa tcggaaagag tcgattcaag ggtacgcgaa
     8581 gaaaacgaca tattagacat gatctctcaa aaaaaaaact ctcatctagt aatggataat
     8641 aaatcctcta agatgagagt tttttgtcct aaaaagagct gcaccaacat cttctggcac
     8701 agctctaaca gggggcaatt tatggaagtt tgatgacttg ttctgtttct ttatcaaaga
     8761 agtggccttt gctgatgttg aaggcaaggt cgactatttc gcctggttta tggaaatctc
     8821 ttgcatctac ttttgagatg aactcagtag aaccagtttt tgtgtaaagc atcgtttcag
     8881 ctccaagtaa ttcggaaacg acaacttctg atttcactac ggcttcaggt gaagcttcaa
     8941 gggctacttg ttcactgtgg atgtcttctg gacgaatacc gaagataact tcttttcctt
     9001 cataaccttt ttcaactaat actttgtttt ttccttcagg aatacgaagc ttaagcccat
     9061 gtccatctgt gataacacca tttttcaatg tcacattaaa gaagttcatg gcaggagatc
     9121 caataaagcc ggcaacgaac atattgacgg gtgtatcata aacttctttc ggtgtaccga
     9181 tttgttggat aaatccgtct ttcatgataa cgattcggtc agccatagtc attgcttctg
     9241 tctgatcgtg agtaacatag atggtcgttg tttcaagacg ctggtgaagt ttggcaatct
     9301 cagcacgcat ggctacacgt aatttagcat ctaaatttga taatggctca tccattaaga
     9361 agacttttgc atcccgaacg attgcccgtc ccaatgccac acgttggcgt tgtccaccag
     9421 ataaagcagc tggttttcgt ttcaagtatt ctgtcagacc taagatctct gctgcattgt
     9481 ccacacgttt tttgatgtct gctttatcat atttacgtaa tttcaagcca aaagccatat
     9541 tatcaaaaac agtcatatga ggatacaatg cgtagttttg gaaaaccatg gcaatatcac
     9601 ggtctttagg tgccacgtca ttcatgactt ttccgccgat atttagttct ccttcagaga
     9661 tatcttccag tcccgcgatc attcgcaaag tagttgactt tccacaaccg gaaggaccaa
     9721 caaaaacgat aaattcacgg tctttgattg atagattaaa atcagttaca gaataatttt
     9781 ctgcattgtc atatttttt
//
LOCUS       DAZULY010000110         9662 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000110 DAZULY010000000
VERSION     DAZULY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.111"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG81A_001711"
     CDS             complement(<1..182)
                     /locus_tag="ACG81A_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015072.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISG"
     gene            complement(198..812)
                     /locus_tag="ACG81A_001712"
     CDS             complement(198..812)
                     /locus_tag="ACG81A_001712"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFS5015073.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(959..1642)
                     /locus_tag="ACG81A_001713"
     CDS             complement(959..1642)
                     /locus_tag="ACG81A_001713"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS5015074.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1751..2209)
                     /locus_tag="ACG81A_001714"
     CDS             complement(1751..2209)
                     /locus_tag="ACG81A_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFS5015075.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2293..4056)
                     /locus_tag="ACG81A_001715"
     CDS             complement(2293..4056)
                     /locus_tag="ACG81A_001715"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFS5015076.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4277..5737)
                     /locus_tag="ACG81A_001716"
     CDS             complement(4277..5737)
                     /locus_tag="ACG81A_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015077.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5945..6871)
                     /gene="glsA"
                     /locus_tag="ACG81A_001717"
     CDS             complement(5945..6871)
                     /gene="glsA"
                     /locus_tag="ACG81A_001717"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFS5015078.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6894..8321)
                     /locus_tag="ACG81A_001718"
     CDS             complement(6894..8321)
                     /locus_tag="ACG81A_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5015079.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8352..9566)
                     /locus_tag="ACG81A_001719"
     CDS             complement(8352..9566)
                     /locus_tag="ACG81A_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFS5015080.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2856 a         2023 c         1609 g         3174 t
ORIGIN      
        1 ccgctaatcg tttgatctgg catttctatt tccttaccat ctaaagtaac agtagtaacg
       61 acaaaatggt cttgcaatgg cggaacttct aagtactgcc cttgcagaaa atcattcact
      121 gtttcatatt cttttttcat ttcttttgaa tctgtcgatg gtttatagct tatttttaac
      181 ataactagtg ctccttttta ttcgggacgt gcaatcgtga aaatttctcc tattgctgca
      241 taatcatttc ctgccaaacg gcttacagca ttcaattttc ttggatcgat ccttcctgtc
      301 ttatcatcat agactgtttc atttaaatga tatttctgta cttctagcaa aaataaatca
      361 gctgttactt tatttttatt ccgtatctcg acatgttcat ataaggatgt ttcaaaacga
      421 acttttgctt ctgtcaatgc tggtactgaa atgattactg aatcagttaa agtcaagtca
      481 gtcaggtaca gttcgctttc ctcattaggc agattggctg ctgttttatt tacttcttct
      541 acgttgtcct catcaaggat atggataaca gcttctcttg tctcaagaat attctttgct
      601 gtgtctttta ctgcatcttg ttttctctgg atcgccaagg ataagatggg tcgattcgaa
      661 ctgacgatat tgaagtaact gaagggtgcg atattcacta tatcttttgc tgatttagtc
      721 gttactactg ctactggtct agggatgact gaaccaatca gcaacttgta attttcacgt
      781 tctgtcaatt ctgaaggact ccgttcaaac atggtttcgc cttctttcta taaaattcgt
      841 taaaaagcag atcttgaaga caaaatgaaa agtaatgctt ttttcatctt cttagaatct
      901 gctttaaact atctatcaat ttttcttttt tgtcaagaat ctgtgccgtg cgtgtttttt
      961 agttatttgc tttacttgcg gcttcgacat atcttttagt gatcaattgc ggacgggtga
     1021 taatagatcc aactacgact gaaaaagcac ctaattcata tgctttctct acttgttcag
     1081 gagagtcagt atgtccttcc ataattactg ggatatcgac ttgttctatg atttgattca
     1141 ataaatcaaa atcaggtgtc tgcgtctttc gtgaatattc tgtatagcct gctaaagtcg
     1201 tagacaaaag atcaaaacca tcttcttgcg cttgaagcgc ttcctcaaaa gtcgaaatat
     1261 ctgccatagc taaccgaccg tgttcgtgaa tcatttggac aagttcagca gttgtttttt
     1321 ctgtcggacg tttacgagaa gttgcatcca gtgcaatgat ttctgcttgc gtctccaaaa
     1381 gttcttccac ttctttttct gtcgcagtaa taaacacttc tgacccatcg taatctcttt
     1441 tgataatccc tattaccggt aaatcaacga ttcgcttgat ttcattgata tcttctgtcg
     1501 tatttgctcg aataccaact gctccgcctt cttttgcagc caaagccatt ctccccataa
     1561 taaaagaact gtgtagcggc tcgttttcca aagcttgaca agaaacgacg actccgcctt
     1621 tgatttgttc cagtgtatcc acaatatctc ctccagtaat gttatataaa aatacagctt
     1681 catataaaaa tgtaaccctt ttctattcag gagacaataa tgagctattt ctttcagcag
     1741 acctttctta ctaaaagaaa attacttcaa attcttcgta tacgaccagc ttctcttctg
     1801 taaattctat gttcaacgct tctttataag catgtttaaa aaactcagca tggactttcc
     1861 gccaataagc taatgtccga tccccttctc cttctttcca agcatgctct ttcgtgactt
     1921 cactaaaagg caagatggtc acatttatat ttttgatgat acaaactggt tcattttttc
     1981 cattcaggac cacgtctaat tgaccaactt ttggtaaagg ctcattctct tgtttataca
     2041 gatcgtaacc acttgatgta ccagtcttta tcttttttac aactagttcg ctcaactcgt
     2101 ctgccatttt ttctgtatcc ccaaatgaaa agaaacctaa tttttctgat tgataactag
     2161 gatatttttc ttggaacttt ttgacatatt cttctacttg ctgatacata acatccaccc
     2221 ctttgctttt tataatgaaa aaaccatcca gatactagta taaacaatta tctggatggt
     2281 gagcataatg aattattgaa tattcaagac acgcagacta tcttcaacga tttgttttgg
     2341 tgttaaatga taacgttcat aaagctgccc tacaggaacg ctatccgtga attctttctc
     2401 tgcaccaaaa tttaatacgt tcattgaaga atcaccataa taacgactga tattttcgcc
     2461 aaatcctcct gacaaactac catcttctaa tgtgatgact aattcgtgtg tttcttttaa
     2521 tttatctaat gtttctacgt cattttctgt gatcgttcct ggattgatca acgttgcttc
     2581 gattcctttt tttctaaaag cttttactgt ttcttttcct aattcaagaa aaccgcctaa
     2641 agctaaaatt gctacttttc ctccattatt tactgtttga taggctggaa tactataatc
     2701 atttgcgata gctgtaccat gttctacacc atgtgtagga attcggattg ctacaggctg
     2761 cgctgtttgt tcgaacgccc aatctagcat agagatcata tcctcttttg ttgctggagc
     2821 aagatatttg atgttcggga tacttgataa gtaaggaatg tcaaatgttc cttggtgtgt
     2881 tcggtcacta gctgagatcg ttccgccacc gactaaaata atcactggat tctcgttgat
     2941 cgctaaatca tgtgataatt gatcatatgc ccgttgcaag aaagtacttg attggaagac
     3001 gactggacgt actccctgag aagccaaagc agaagcgaaa gtgatagagt gctgttcagc
     3061 gatccccacg tcaaaataat gatctggatg tttttcttgg aatgttttca gtccaaaggc
     3121 gcctggtatc gctgcattga ctgcggcaat aggagctgta cttgtttgca ttttcttatc
     3181 caaataatcc atgatgatca tattaaatgt ttctccagaa gttacttgct ttgcctcccc
     3241 ggtggacaga tcaaatggta catgccaatg atatttcatt ttattatcga ttgccggctg
     3301 ataaccgcga cctttttccg tatggacgtg aagtacaatc ggatgatcaa tatcttttac
     3361 ttcttcaaat aaacggatca ccgtttccag atcgtttcca ttatccacgt aacgataatc
     3421 aaatcccatt gctttaaata ggttattgct gcttttaccg ttggtttgac gaagctcagc
     3481 taagttttta tataaactgc catgattctc atcaatagac atctcattat cgtttactaa
     3541 tacgattaga ttggattgta aaacaccacc attgttcaat ccttcaaaag ctaagccacc
     3601 acttagggag ccgtcaccga taaatgctac cacatttcct gattctttct tcagatctct
     3661 cgctttagct aaacctgttg ctaagctgat agatgtagat gtgtgcccca ctgtaaaata
     3721 gtcatgttta ctttcatgtt ggttggaata tccgctcact tcacggtaat gtcctttttc
     3781 aaaaccattt tttcgaccag ttaaaagctt atgaccataa gactgatggg aaacatccca
     3841 aacgatttta tcctttggtg aatcaaatac ataatgaaac gcaatcgtta attctactac
     3901 acctagattc ggaccaacgt gcccacctac atcgctgtcc ttttgcaaga ttttttggcg
     3961 catctcttct gccaactgtt tcatttcctc aacagataat tttttgacat ctgcggggct
     4021 attgattgca tccaatacat gtaatactgt actcataata cattctctcc tccgtactat
     4081 cttcttttaa tgaaaataga tttgttacac tttatctatt ctactactta gagttgactc
     4141 taagtcaata gcattttaaa aaaatttcag agaaagcttg ttatataagt agatcttcgc
     4201 acgaatcagg actcaaataa aaaccttcta cagacaatta aaaagctttg tagaaggtct
     4261 cctaattaag gtctcatcaa gaaatatgtt tttttaatag gtgaagttcc tgagcaatat
     4321 gatacaaatg gatgtttgca ccttttaatg agattggata gatctttgtc ttttcagttt
     4381 ctaatccatt ttgatagcta gcagcatcgc acaaaaccac tctttttttc gcacatagac
     4441 attcatcagg caattcttct aatgaacgaa ataaaatagg atttatttca gcgatgcctt
     4501 cttccgcata caaatgaaat tgggttcgat aaaaaaattg tttgactgaa ctttctgaaa
     4561 caacaaaaat ttctatttcc tgtctccccg cttgttcact gttgatcaaa gaaagacttt
     4621 tgacgaattt tcgaatgaaa ttttcattcc atcttagttg gatttgattt ttctttttcc
     4681 actcttccaa tatttctaca atttttccgt acagaagttg ttctttttct gatagaagat
     4741 agatcctttc cggtaaaaat acttgtccat ctgcccacaa cgtacggaca aattgtaaaa
     4801 aggattgctt gaaaataaaa ttttctgcat cgtcgatttc agcgatacta ataatctgta
     4861 aaatcaattc attcagtgat aggtcattga gaacaaaatt tttataaaca acttcgaaat
     4921 cttttcttaa cagctccatg ttctcattgg tataatttct actgttgaac aaagaaaaaa
     4981 tataatagac atcttcttct tgtaagtcta gaattccttt ttgctcttcc aatccttgac
     5041 agactaattg aaataacggc ttcgtttctg cgcttttctt atcttcttct ttcagtccaa
     5101 ggggaaatgc ctttcttccg attgcgatct ctattcctcg tatgataaag gacttttcat
     5161 cttccgaata tcttctttta aaaaaatgag tctctacata atcaatcaac tcgttacaag
     5221 cttcttctac atacggattt ttattttcat agccttccca attgacataa cgtactaaag
     5281 ctagcaagat gttccgcgag tcaaattctg ggataatgat ttctttattt ttcaaataat
     5341 cattttcttt aaaaaaatcc aatacgattt ttttgattgt atatactttg ctcaaagaaa
     5401 taaattcaag atcagccagt tctgtactcg tgaatatacc attaaaataa taacttaaaa
     5461 actttaaaaa ataagattga ccgtacagaa actgtgtgag ctcagaaaga gcaataccat
     5521 gtttgaatac gagtttatac ttatgcttct ctcttacttg ttcttttata caatattcta
     5581 gacactcggc tagactttct agatcgtgaa ggatcgttgg tgtagtcacc cttagagaaa
     5641 tagctgtttg ttcgatgtct atttcttgga attcaaataa ttgttcacac agatacactt
     5701 ttctaaatat atttcgctcg atgtaatttt ccagcattcc tgatacacgc ctccagtttt
     5761 tcttatgatt ttatttaatc atatctgtaa atcgtatttt tgtttccttt ttatttagaa
     5821 aagccctttc aaaaaccaat aaaaaaagaa actgggacaa aagtcaaaca agctcacaat
     5881 ttcagcctca ttgtaggtag ctttgagccg tttggacttt taccccagcc tctttcaaga
     5941 atggttattc aaagatgtca gcgtctagtt ctttgactac atctttcaac aattggatac
     6001 cagctgcgct gttaccaaat ggatctagtg ccgggctgaa gacacctatg ccgtaacgat
     6061 ttggcacagc tgccatcaac ccgccgccta caccgctttt tgcaggtaca ccaacatgga
     6121 cggaaaactc tcctgattca tcatacaaac cagcagttgt cataattgat ttgacgattg
     6181 tagcactttc ttcagtaatg atttgtttgc cattccaagg agcaattcct ttatttgcca
     6241 gcacagcagc taattttgct atgcctgtag cggtgacatt gagggagcat tgtctgaaat
     6301 aagcatcgag tatctccggt acgtctcctt cgatcatttg attacctttc atatagtaag
     6361 caagagagcg attgatatca cctgttcttg attcagaatg atagatttct tcattcaatg
     6421 tgatattagg atcatcacag atctctttca taaatgacaa gattcgctca aatttttcat
     6481 ctgcatcttt accggcaatc aacgaggttg ttgcaatcgc tcctgcatta acaaaaggat
     6541 tcattggatg tttgcgatga ttgatttcca tattcatgat agaattgaag gcaaaaccgg
     6601 ttggttccgt gttgattgtt tgaaagactt tttctattcc gttgtctttg attgctaata
     6661 acaatacggg aacttttgac atgctttcga tggcaaagcg cacatcggcg tctcctccct
     6721 caatcagttg ttttgtatcc aaatcataaa tcgccatacc taattgtttg ggatccactt
     6781 cagctaatgc tggaatgtaa tccgccactt gtcctgtttg ataatagtgg aaattttttc
     6841 caatcaattg ttctaattct tttacttcca tttgaataac cagctttcat tttttatttt
     6901 tctgcatgct ggaatttatg acggcatgcg tagatcgtaa atggaataac taagacgata
     6961 agataagaaa caatcagaat cgtttcataa atcccatgct gtccagctgc gatactagca
     7021 ggtggaacaa atgagataca aaaagcaaaa agtgaagtca aaagacctaa acaagcaacg
     7081 attactttta caacttttcc gcctggaacc tgataggctc tttcaagact gtcatcttta
     7141 aaaaccagct tcaaatagcc cgcgaaaaac ataaagtaca acatcaggta gattacaaca
     7201 gtcaatgaca tcgctgcaag aaatgaaaga ttattccccc cgccgcttaa tgtaaagacg
     7261 gctgcccaga ttgttgcaac gatcccattt gcgatcatca aatgagcagg aacaccgtgt
     7321 gtgttttctt ttgcaaaata ttcaggaagt aatccttctt ctgctgctac gtgaagtgct
     7381 tttgaaggag aggttaccca agaagcaatt tctccgatga cacctaaaca aacaagaatc
     7441 gcaagaattt tcgcaatcca tgcaagattt ggatcaatgt gtcttaatag gaattctaac
     7501 gattgaatca cccctgtatt aagagacaaa ccatgttgag gtactgtagt agcgacggtc
     7561 aatccgccaa aagtatctaa aatgattgcc agaataacca atacaaacat tgctaatgga
     7621 taatcctttt tagggttttc catttcattt gcatgactgg cagatgtttc tacgcccata
     7681 tatgctaaaa taaaggaaac aaaaacaacc agcgtaggca atttcgaaaa atcaggaatc
     7741 aatgttttca tagacagtat cgtttgtagt ggagcgcctg aagcgacata atggatccct
     7801 aagccaaaca agactaatgc tggcaacaag ataccagcta caaaaccgat ccgagcaatc
     7861 aatgaagtgt atttggttcc ttttaactgt gagagtaaaa tagcccagaa aataatcaat
     7921 aggattgcca atttcagata tttattgttt tgtatagcac caatacccgt tgcataggat
     7981 aatgcaccag ttaagaaata cagcatggtc aagtagccaa ctgttatctc aaaccattgg
     8041 aaaaagattg cggcaaaacc gaatcgtttt cccaatgtgc gactgaccca agtatagaca
     8101 ccgccttttt cccatccttt caccgttgcc atctctgctg cgcacaatgc cacagggata
     8161 aaccatagca aaccgcctaa taacaagaaa aatactaagc tgaagcctga ggtcgcgaat
     8221 gtgggatatt catataatga aacgaccatt gagatggtca tagccaaaaa gccaaagaac
     8281 gttaattttt ttgcaggaat tttcgttttg ctttgttgca tgaatcttcc tcttttctat
     8341 ttgaatttgt ttcaattgag tttgttgcca aagaagaaaa agtccaatcc ttcttcttgt
     8401 tcttcttttg tagtagtgat cgttgtaaag tgcatcgccg attttaaaat cactgttgtc
     8461 agcacatagg catacacggc agtaatgatg acaccaatca cttgaatccc taagaagtac
     8521 cagctgcttg gattcgttag taaatggctg ttagatgcaa agatccctat cagaatcgta
     8581 cctgtgaatc cgcccatgcc atgaacaccc cacacatcaa gtgcatcatc ccattgtttc
     8641 tttttacgaa aatcaacagc agcatgacag acgatacccg caatgatccc aatcagcatg
     8701 gcactttttg cttctacata accggcacaa ggcgtaatgg ctgccaatcc agcaacggat
     8761 cctgttagta cgtcgacaaa atcaaagcca tttcctttta cttttgcaat gatcagccaa
     8821 gtaatcatcg caaatgcaag agcgatgaat gttgaaccaa atgcattgac tgcttgttca
     8881 tctgctcgaa gtgcaccacc ggaattaaaa ccgaaccagc caaaccaaag cagtcctgta
     8941 ccgatagcag ctgccataag actgctatgc tgacttcttt ctcctaaagg aatctttctt
     9001 tgaccaagtg tgaggataca tgctaaagag ccgaatccag ctgtcgtgtg gatgactgct
     9061 ccaccagcga aatctctaaa tcccatctga tccaaaaatc cgccacccca tatccagtgg
     9121 caaacaggga aataaatcag cagattccaa aaaataacaa gtataatata gccttttaaa
     9181 ttcaatctcc ctgcaaaagc tcctgtcatc aaaggaacgg taatcacgat aaacatcagt
     9241 tggtaaagaa agaacattaa aaatggaatc gttgcaccat gaaacatgtt ggggaagctt
     9301 gtaacatgtc tcatcaaaaa gaaatctgcc gggtttccaa taacaccgcc gatatcccgt
     9361 ccaaaggcca atccgaatcc accaaatacc cacataatcg ttgttatccc aacggagaga
     9421 aaagattgag acatgatcgt tagtatatta ttcttccgtg ctaatccgcc gtaaaaaaaa
     9481 gctagaccag gagtcatcag gcaaaccatt gctgtacata aaatcatgaa tgctgtatct
     9541 cctgtattga taggtaatgt attcattttt ttcgcctcct ttctacgatt acgagtataa
     9601 aagaaggtgt agttgttttt ttcttctgtt tttttgaatt aatacttgca tttataaaag
     9661 ac
//
LOCUS       DAZULY010000111         9629 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000111 DAZULY010000000
VERSION     DAZULY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.112"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(10..672)
                     /locus_tag="ACG81A_001720"
     CDS             complement(10..672)
                     /locus_tag="ACG81A_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5015081.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(685..2187)
                     /locus_tag="ACG81A_001721"
     CDS             complement(685..2187)
                     /locus_tag="ACG81A_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5015082.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            2735..4414
                     /locus_tag="ACG81A_001722"
     CDS             2735..4414
                     /locus_tag="ACG81A_001722"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS5015083.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            4541..5020
                     /locus_tag="ACG81A_001723"
     CDS             4541..5020
                     /locus_tag="ACG81A_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFS5015084.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            5239..5919
                     /locus_tag="ACG81A_001724"
     CDS             5239..5919
                     /locus_tag="ACG81A_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS5015085.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            5927..7258
                     /locus_tag="ACG81A_001725"
     CDS             5927..7258
                     /locus_tag="ACG81A_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFS5015086.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            7273..8235
                     /locus_tag="ACG81A_001726"
     CDS             7273..8235
                     /locus_tag="ACG81A_001726"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFS5015087.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            8346..8684
                     /locus_tag="ACG81A_001727"
     CDS             8346..8684
                     /locus_tag="ACG81A_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFS5015088.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            8763..9470
                     /locus_tag="ACG81A_001728"
     CDS             8763..9470
                     /locus_tag="ACG81A_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFS5015089.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         3057 a         1735 c         2071 g         2766 t
ORIGIN      
        1 tcctgttttt taaacttcga ttttttgaaa ttttttattt tttagttcaa agacttcatc
       61 acagcttttt gctacttcaa tctcatgagt cacgatgata atacagcgat tttgttcatg
      121 ggcgatttct tggaaaagtt tgacgatatc ttgtgaagtt gtttcatcta gatttccagt
      181 aggctcatcc gctacgatta gttgatgttc acagcagata gcacgaacga tagcgactcg
      241 ctgctgctga cctcccgaaa gtttggtcac ctgttttttt gctaatattt catcaattcc
      301 tacacgagcc agtcgatcta aagcaacttg tcgataattc tcttgcttgg tcttagtgat
      361 ttccattgct gctatgacgt tgtctagagc cgataaataa ggaagcaagt tgtaagcttg
      421 gaaaataatc gagacatcat ttttacgata acttcttaac ccgattttgt tcagtgaacg
      481 atcttgatag tatacctttc cttcttttgg tgcatctagt ccagaaataa gcgaaagaaa
      541 agtcgttttt ccagaaccac ttgctcctaa aatagcgtac atttttccag cttcgaaatt
      601 cagttctaca tctttataaa gcgcatcttg cggatcttga taccaaaatc ccaattgttc
      661 tgtccgtaac atcaaattcc tcctttagtt taataggatt tttcttgggt ttaatcttaa
      721 tatccctata gaagctaaaa agacagaaaa cagactgatt ccaaatgcta ctccggctaa
      781 tagtcccaat tgtgcaggtt tgacattgat ttccaattcg ctgacttgat cagaaacagc
      841 aaatggatta ttcgtggttc cttgttgacc atcgcgttga ccgccttgtt cttgtctgtt
      901 acctggcatt tgtccaccgc caggagcttg ctggttttgt gtcgtggttt ctgtctgttg
      961 gtcaagtagc tgctgtccca cggcattcgc gacgacgttt ccgctaagcg aagcaactcc
     1021 taatgcaaga atcatacaaa tagccacttc ggtaaacaat tggagaatga ttttacttct
     1081 ggattctcct aaagatagca agacgcctaa ctcatgccga cgctcccgaa tggtaatcat
     1141 aatgatcaga gtaagtatga taataccggc tacagccact agtaaaacaa catttttaga
     1201 aaaacttgcc acgttgttta aaggttccaa cattgactga tacatggaat catttgattg
     1261 taagctaaat gtatctgtgt cgatcagctt ctcagcttct ttgagaaact catccatttt
     1321 atctggatca gatagagtgt aaatagcaga atctatcgtg ttatcgtcag atgtcccatt
     1381 tagttcattt gcaaaagtgt aagaagtgta aagtgtattc gacggattca tgaagttgaa
     1441 tttcataccc atactgccgc ttgtttcgct ggattcataa attcctttga tcgtcatttc
     1501 ataagtggta tcttcatctt cagtactcgt gattgtgaaa gtatctccga ctgccaggtc
     1561 atttgtttct gctagagtag aatcaatgac tacattgttt gtgcctttgt cgctgtcatc
     1621 tattccttca ccatccgtga tttttgctgt tccatcagaa aaattactat tttgtgcagt
     1681 tgctgacaca ccactgattt gaaagtctgc ttgagtcatt tgcggattcc cgcccattcc
     1741 tccagcaggt tcaccgcctg catcagtagt ttctgaactg tcttcacttg aatcagaact
     1801 agtgatggca gttattccat cttttgccat agctgaggcg gaactttcaa aggaataact
     1861 ctttacatta tctaattcag ctattttttc cgcgtccgtt aaggaaactg gtgtaagaga
     1921 aaaactgcct ggatctggac gttcttcagt ggtagaaccg cttgaagaag tttgttcctg
     1981 ttttttaaat gaagcttctc gattggcact taaagtcact gttgctccaa cttctttttg
     2041 tgcttgattc gctgcttctt ctgcagcgct tcgaatcgtg aggccagcta gcacaaaaat
     2101 aagaatggct gtgaacacgg caaacaataa caatgaacgt ccccatttta cttttgtact
     2161 tttccatgca cgtttcataa aattcatatt ttgcatccct cctagtagat gcatttgata
     2221 atagacaacc tatcttaaag ctagcttaaa tcttatgctg tttgcacacc atcaacatga
     2281 ggttctgtat atataaaaag tcactatagt tattgcaaaa aatgtggtaa aatgaaaaaa
     2341 gtatgtttca aaccgacgaa gaagaaatga aagaggaata gcaaaagaaa tcctagatgc
     2401 tacagaggga tttttacgtg catagttgtt ctaacaattg ctgatactag cgatttagtt
     2461 tatttgttgt ataataatgg ggaacagctg aatacttata taagtattct ggctatagcg
     2521 tttcgcaaaa gatccatttt gttaatgaag tatatccaaa ataaaatgcc catccaactg
     2581 tcagaacgcg gatgtgctgg taattttcct gctgattctc cataagttga gaaatagtga
     2641 ttttgcatat gcttcattgg aatggctcat atatttagat aaagaagaaa atcgacatct
     2701 ttaaacttgg atgtcataag aagaggtgaa gtttgtgagt accattaaaa ttatcccatt
     2761 aggcggcgtt cgtgaaaatg gaaaaaacat gtatgtcgca gaagtgggcg aggatatttt
     2821 tattttagat tgtgggctaa aatatcctga aaatgagttg ttggggatcg acgtcgtaat
     2881 ccctgatttt acgtatttag aagaaaacat tgatcgtgta gctggtgtct ttttaacaca
     2941 tggacatgca gatgcgatcg gggcacttcc ttacttgttg tcaaaaattt ctgtaccagt
     3001 attcgggaca gagttgacga tcgagttagc aaaattgaat gtcagtcgta atgaagcagc
     3061 caaaggattt aaggatttcc atgtggtaga tgagcataca gaaatagact ttggtgaaac
     3121 agtagtgagc tttttccgta ccactcatac gataccagat tcagtgggga tcaatctgaa
     3181 aacaaaagaa ggtagtatcg tttatacggg agattttaaa tttgaccaaa cggctatccc
     3241 aatgtatcaa acagattatg cacgattggc ggaaatcggc aaagaaggag tcttagcctt
     3301 actaagtgaa tcggcaaatg cagaaaatcc tcagcctgta gcttctgaag cccaaatagc
     3361 agatgaagta tttgatacga tcaaatattg ggaagggcgt atcatcgtcg cttgtgtagc
     3421 aagtaatttg caacgagtgc aacaagtatt ggatgcagca gaaaaagcac aacgaaaagt
     3481 tgtcttgact ggtcaagatt tcgaacgaat tattcgcaca gcgatgcgtt tagaaaaact
     3541 tcaattacca agtgaagatc tgcttgttac gttgaaagac atgaaaaact atgatccaga
     3601 agaactgatc attttagaaa ctggccgaat gggtgaaccc atcaagtctc tgcaaaaaat
     3661 ggcaaacggt acacaccgta atgtcaagat tgaagaagga gatttggttt atatcactac
     3721 tacgccaagc atcgcgatgg aaaccatcgt ggcgaaaacg gaagatatca tttatcgagc
     3781 tggtggaaca gtgaagttga tctctgagaa tatgcgcgtt tctggccatg ccaatcctaa
     3841 tgacttgcaa ttaatgatca atttgatgaa accaacatat tttgtgccag ttcaaggaga
     3901 gtatcgtgaa ttagctgctc atgcagattt ggctcatgca gtagggatgc catacaaaaa
     3961 tatctatatt actggacgcg gagacgtgct ggaatataag aacaagcgta tgagtgttgc
     4021 aggaactaca agtgcagaaa atgtaatgat tgatggatta ggcataggtg atatcggaaa
     4081 cattgtttta cgtgatcgaa aaatcttgtc agaagacggg attttcgtag cggttgtcac
     4141 tatcagtcgg cgagaaaaga aaatcatttc aaaacctcaa attacttcac ggggattcgt
     4201 ttacgtaaaa gctagccgtg atctgatgaa agaaagcagc aatatgattg aaacaatcgt
     4261 tgagaaacat ttagaaagca atgattttga gtggagcaaa ttaaagcaag acatccgaga
     4321 tcaattaggc cgttacttat ttgaacaaac aaaacgccgc ccagtgatct tgccagtcat
     4381 tatggaagct actcaacgtc gaaataaaaa ataaacaaaa agagtaagat gaacctctct
     4441 atagagtgct cgtcttgctc tttttttgcg aaaaacaaat aatttttgaa tacagcatcc
     4501 ctcgctttat aataaacgat agaataaagg gagtgaatca atgatgagtc aaacaaaagt
     4561 cgaacgttat ttagaagaac aaaagatcag ctatcaacca cttgatttta gtcatgtggt
     4621 agaagaatct ttcacagaag aactacaaaa aagagggatt gatccagctt tgatatgtaa
     4681 aactctcgta gtgaaaggaa acaaaactgg ggtgatcatc gcattgattc ctttgaacga
     4741 tcacttagat tacaaaaaaa cgagacaagt gacgaaagat cgtaaagttg gttttcctgg
     4801 aatggatttt gtattaatgc atacaggata tccccacgga gcaaacaccc ctattggaat
     4861 aaaactcgca catcctgatt attgtttttt agcagacagt tctttaaaag aaaaagaaga
     4921 agtaattgtg tcaagcggtg aaatcgggag aagccttcgg ataaaagtag ccgatttaga
     4981 aaagatcgtc catcctattt acgctgattt aattagttaa gggacgagaa gagaagctaa
     5041 gcagagcaga gacctcaaac ttgagattat atctgcttgg tacttggtgc atcctgtatt
     5101 atctgttaca attgctttgt acatactgta ctatcaatcc aatgaaaata tatttagcag
     5161 ctatattttt tcattgatgt gcaagtgatg aaatcgtttt taataaaaaa gatacagaga
     5221 aaacgcgagg tgtaaaatat gaaaattacg tatcatggac attcctgttt aacaattgaa
     5281 atggaaaatg gccaacgcgt gatgattgac ccgtttatta caggaaatcc attgacagac
     5341 ttaactgcag aagaagtaaa gacagattgg ttgctgatta ctcatggtca tagtgaccac
     5401 ataggagata tgattcctat tgcaaaagaa aatcaagcca caattatcag tattgttgag
     5461 attgctgatt atgcgaacag cagaggagta gattcattcg gtatgaatat cggtggaaaa
     5521 catgcgtttc cattcggcgt ggtaaaattc gtacatgcac agcatagttc aagctatgaa
     5581 gtagacggta tcgttcagta tatgggcgag cctagcggaa ttattattca agcagaagga
     5641 aaaacgattt atcatgcagg ggatactgct tatttcagtg atctaggatt gcttgcagaa
     5701 gaattcgata ttgatgtcgc tttcttgccg attggcgata actatacgat ggggccagaa
     5761 gatgcagcaa aggctgcgca aacaattcgt gccaagaaag tagttccaat ccattacaat
     5821 actttccctg tcattgaaca agatcccgaa tcttttgtag agttactgga agatagtgaa
     5881 ggaaaaataa tgagcgtctc ggaaacgctg gaagtgtagg cagaaaatgg cgacaaaaca
     5941 tgatttgatt ttagaatata ttgaaagttt acctgttggc aatcgaattt ctgtaagaag
     6001 catcgcaaaa gaattgaatg tcagtgaagg aacagcgtac cgtgcaatca aagatgcaga
     6061 aaatgtcgga ctagtttcaa caattcaacg agtagggacc atccgtatcg aacgcaaatt
     6121 aaaaaaacat attgaacgtt tgacatttgg cgaggtcgtc aggatcattg aaggggacgt
     6181 tttaggcggg gcggcaggat tagacaaggt cttgaataaa ttcgtcatcg gcgcgatgac
     6241 taaacatgcc atggaacgct atattacgcc aggatcacta atgatcgtcg gcaaccgggc
     6301 ggaagtccaa aaattagcat tggaagatgg cgccgcagta ttgatcacag gaggattcaa
     6361 tacgactgct gagattgcaa agctagctga tgatcttgaa atgccgatct tacgcacctc
     6421 ttatgataca tttacagtcg caaccatgat caaccgagcg ttaagcgacc aattgatcaa
     6481 aaaagatatc atgcttgtca gtgatatcta tatgccagct gaaaagactc attatttgca
     6541 gcagacagat acgatttctg attatcagcg tttgtcagaa gaaacgcagc attcgcgttt
     6601 tccagtcgtc aaccgccatc atcgtttgat ggggattgtt acggcaaaag atgttttagg
     6661 gaaatcgcca aaccagatca tcgatcgggt gatgacgaaa gaaccaatca gtgtaaagaa
     6721 aacaatgagt attgcctctg ttagccatca aatgatctgg gatgggctag aagtcatgcc
     6781 ggtcgtttcc gatgacttga cattggaagg cttgattacc cggcaagatg taatgaaagc
     6841 gatgcagctt gtccagcgac aaccgcagat tgcagatacg atttcggatc agatatctgg
     6901 aaatatccat acgattgata ctgaccgtga aggaaatcgt ttggaaaacc ctaagttcaa
     6961 attcgtagta gcaccacaaa tggtcaatgg cgttggaacc atttcttttg gcgtacttag
     7021 cgaaatcatt tctgatgtcg cacaaaaaac aatggtgatg aaccaaaaac gaaatatctt
     7081 gattgaacaa gtgaacctac attatttgcg gctgatccaa ttggaaagcg aattagacat
     7141 tcgtccacgt gtattggaaa tcggcagacg ttccgctaaa ctggatatcg aagtgtttat
     7201 tgaaaacacc atcgtttcca aagcaatcgt tgtttgtcaa gtaatggaac gttcttagaa
     7261 agcaggagtg aaatgacagt acagcaagaa atagtagata caataaaaaa atacgataaa
     7321 atcattatcc acaggcattt gcgccctgat cctgatgcat taggatcaca agtaggatta
     7381 gccgaactat tacgggaaag tttcccagaa aaagaaatac gtcaagtagg tgaaacgatt
     7441 gaagggcttc gctttttagc agaaatgcaa gaagttgaag atgtcttcta ccaaggagcg
     7501 ctagtcatcg tgactgatac agctaattca cccagaatca gtgatcaaag atatcagttg
     7561 gcagaaaaac tgatcaaaat cgaccatcat cccaatgatg acccttatgg agatcttgtg
     7621 tgggtaaata caaaagcaag tagctgtagt gagatgattg cagaatttgc tttgatgttt
     7681 cctgaatctt taaagatgaa cgttaaggct gcacggttac tttatgcagg aatcgtcgga
     7741 gatacagggc gctttttgta tccctccacg acgtcccgta cattagagat tgcttcgata
     7801 ttgagaaatt atccatttga tgcaagtgct ttgaacagag agattgaaca aatgccgatg
     7861 aaagtcgcta aactttccgg ttatctttat caaaatatcc aggtggatga aaatggtgca
     7921 ggaagaataa tccttccgca agaaattctt gataaatatg gaatcgatga ttcagagaca
     7981 gcttcgatcg tttcacttcc tggagtgatt gacgaagtgc tggcttgggg gatttttgtt
     8041 cagcagcctt cagggtatta ccgcgtacgt ttacgttcta aggggccgat catcaatgaa
     8101 ttggctaaac gccatcatgg aggaggccat ccattagcaa gtggtgcgaa tgcaaaagat
     8161 ttagcagaag tagaggaaat ctataaagag atccaagata tatgccaatc gtataagagc
     8221 agaaaaacag aatgatactt cgttcgaaaa cgtgcctttt gtagtaaaaa tcacaaatgt
     8281 atcttttcaa gatcttcttt ttccgttaca atagtgctca ctaaagattt taaaggagaa
     8341 agaagatgac attaccaaat tgcccagaat gtggctcaga gtacacatat gaagatcgag
     8401 gcttgttgat ttgcccagaa tgcgggaacg aatggaacaa agaagaacaa gaaactgtca
     8461 tagaagacgg attagtggta ttagatgcaa atggaaaccg tctgttagag ggcgatagcg
     8521 tgacagtgat taaagacttg aaagtaaaag gtgcgtcagg tgccattaaa caaggaacaa
     8581 aagtcaagaa catccgttta gtagaaggcg atcataatat cgattgcaag gtagaaggat
     8641 ttggaccgat gaaactgaaa tcggaatttg tgaaaaagaa ttaaagaata ttcaaggtct
     8701 tttcacattc ttttcctact actaatgata aaataaagta aacgaaaagg agtgaaacga
     8761 aaatgtatgg ttatggaatg tatggttttt tagatccgac atttcttttg gtcattcttg
     8821 gattagttat ttccggaatt gcgtcagcgt acgtcaatag tactttccga aaatacgatc
     8881 aagtgagaag caaaaacaat gtgactggta cacaagcagc acaatatata ttacaaagtc
     8941 aacagataaa taatgtcggt gttcaacaaa ttgccggaga tctcacagat aactacaatt
     9001 caggtaataa gatgttgagt ttgtctcaag caactgcaca gtctacttca gttgctgcga
     9061 taggtgtagc agctcatgaa tgcggacatg cggtacaaga tgcttcgggt tatgtcccat
     9121 tgaagattcg tgcagcactt gttccagcag ctaactttgg ttcgatgatt tcgttcccct
     9181 tgattatggt aggtgtattg cttagctgga actctacgtt aatcaatatc gggattttcg
     9241 ctttttcttt agcattactt ttccaattgg tgacattacc agtcgaattt aacgcgtctc
     9301 ggcgagcaat ccaaattttg agtgaaggcg gactactgac ggaagaagaa gtaccgatgg
     9361 caagacacgt attatttgca gcagcactga cttatgttgc agcagcgctt tcgacattcc
     9421 tgcagctgtt gcgcttgatc ttattgttcg gcggcaaccg aagagattaa aataaagaag
     9481 ttgtgaaatc agcgctttga tctgagctaa taccgagagt tagctgattg aataccattt
     9541 atttggaata agggctcttt gtcaataagg actgatgggt tgtacaaaat caaatctggg
     9601 tcagaatgaa cctcattctg gctcagatt
//
LOCUS       DAZULY010000112         9600 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000112 DAZULY010000000
VERSION     DAZULY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.113"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            167..2095
                     /locus_tag="ACG81A_001729"
     CDS             167..2095
                     /locus_tag="ACG81A_001729"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5015090.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            2233..2688
                     /locus_tag="ACG81A_001730"
     CDS             2233..2688
                     /locus_tag="ACG81A_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFS5015091.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            2768..2896
                     /locus_tag="ACG81A_001731"
     CDS             2768..2896
                     /locus_tag="ACG81A_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015092.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            3158..3427
                     /locus_tag="ACG81A_001732"
     CDS             3158..3427
                     /locus_tag="ACG81A_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015093.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            3827..4081
                     /gene="rpsT"
                     /locus_tag="ACG81A_001733"
     CDS             3827..4081
                     /gene="rpsT"
                     /locus_tag="ACG81A_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFS5015094.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(4170..5216)
                     /gene="holA"
                     /locus_tag="ACG81A_001734"
     CDS             complement(4170..5216)
                     /gene="holA"
                     /locus_tag="ACG81A_001734"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFS5015095.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(5254..7527)
                     /locus_tag="ACG81A_001735"
     CDS             complement(5254..7527)
                     /locus_tag="ACG81A_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFS5015096.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(7520..8059)
                     /locus_tag="ACG81A_001736"
     CDS             complement(7520..8059)
                     /locus_tag="ACG81A_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFS5015097.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(8195..8413)
                     /locus_tag="ACG81A_001737"
     CDS             complement(8195..8413)
                     /locus_tag="ACG81A_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS5015098.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(8410..8655)
                     /locus_tag="ACG81A_001738"
     CDS             complement(8410..8655)
                     /locus_tag="ACG81A_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFS5015099.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(8771..9499)
                     /locus_tag="ACG81A_001739"
     CDS             complement(8771..9499)
                     /locus_tag="ACG81A_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFS5015100.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         2969 a         1863 c         1665 g         3103 t
ORIGIN      
        1 aatgttctgg ttttttattt tctttaataa taatcattat aaataataaa aacaacatca
       61 agcttaatca ccaaatgata accattatcg ttctcattta gctgttatta ttatcaatta
      121 catttttatt atgctatcct tgttttgaca aggaggtcta ataacgatga ctaatcagaa
      181 aaaagctttt gcagcaacaa tcggctgcct tttatttatg atcttcggtt ttatcttcga
      241 ccgattctct cttaccatca ccccactttt ttatatcagt gccattatca tcggtggata
      301 taaacaaaca tcggaaggct tgaaagaatt gtggaatgat cggacactga atgtggattt
      361 attgatggct ttagctgcta ttggtgcctg tttgatcggt aattactttg aaggagcaat
      421 gttgacattc atcttttgtt taagcggtgc attagaggaa tacacaacta ataaaagtca
      481 aaaagaaatt actgctttga tgaatcttca accacaaaaa gcccaattat taaaagaaaa
      541 tggacaaatg caagaagtag atgtttctca actaaaggtg gataacttgg tatttgtcgc
      601 taaaggagca gctgttccga tagatggctt tttagaaagc acacaggcaa cgatcgatga
      661 gtctgcatta agcggtgaat ctgtgcctgt agaaaaatat ttaggcgatg agatgtatgc
      721 aggaacgctg aatcaaggaa atcccatcac gattcgagta acaaaaacga gttccgaaac
      781 tgtctttgct aagattatcc aattagtaga agaagcgcag aatacgccaa ctcaaacagc
      841 ttcatttatc gaacgaatcg aaaataatta tgtaaaattg attttgcttg ctgttcctct
      901 aatgatcctt ctcccccact ttttcctagg ttggagttgg gatgaaagct tttatcgagg
      961 aatggtatta ttagttgtgg cttctccttg tgcgcttgtc gcatctgcta caccagcttc
     1021 tctagctgct ctttcaaatt ctgcaaaaaa tgggatttta atcaaaggcg gtatccattt
     1081 agaaaagctt tctgaattaa aagcgattgc ctttgataaa acaggaacgc tcactaaagg
     1141 aaaaccagtt gtaactgaca gctttttctt tgatgaaaag gatttggcac aacaacttct
     1201 tgttgctatg gaacaaaaaa cgacgcatcc acttgctcat gcgattttgc aatatttcga
     1261 ttgcacaata cctgaagaaa tccaatactt accaatagaa gaaattactg gttttggatt
     1321 acaaactacc tatttaggtg cgcaatggaa agttggcaag cacgcatatg atcctgagac
     1381 gatgataatc agcaaagaga ttgcggaaat gatcgaacgt ttggaaaatc aaggcaaaac
     1441 cgtgatttat ctttctaaag atcagcagtt aattgctgtt ttaggtctgc tagatattcc
     1501 aaaagcaaat acccaacaag taatttccta ctttaaatcc caaaacatcc atacaagtat
     1561 gataaccggc gaccattctg gcacagcaaa ggccattgct gaacaagtag ggattgaaca
     1621 ttactatgca agctgtactc cggaagaaaa aacacaacta gtcaaaaaag agaatgaact
     1681 ttaccaagta aatgcgatgg ttggtgatgg agtcaatgat gcgcctgctt tagcagccgc
     1741 ttctttaggt gtcgctatgg ggcaagggac agatgccgct atggatatcg cagacatcgt
     1801 cttaatgaaa agcgacttaa ataaatttgt ctacagtcat cggctatctt taaaaatgcg
     1861 gcggatcatc aaacaaaata ttattttttc gattcttgtt attctcttgt tgattgcttc
     1921 taacttcctg caatttttaa atctacctct tggagttgta ggacacgaag gaagcacaat
     1981 attggttatt ctgaatggct tgcgtttact aagaccttta cctgatcttt ctgattcttc
     2041 aaaaaaatgt aagtcttgtc cactatacaa agcgcggctg tcttctgtca aataagcaaa
     2101 gtgttttttt atgattttgt ttattttatg aaaaaaacag cttatgaata ggtcttgaag
     2161 gatgaattcc tacttttgta actttctaaa aacatgttat gattgtttcg aagaaagtga
     2221 ggaatatatt tcatggaaag ttggcttttt ttaggaatta ttttacttgt agcaatttta
     2281 gctaaaaatc aaagtctgat gatagcggca gcttttgttt tgattatgaa gctcatacca
     2341 gcctcccgtt cctttttccc ttggcttcaa ggcaaaggaa tcaatattgg ggtgacgatc
     2401 atcacagcgg ctattctaat ccctatcgca acaggagaaa tcggtctacg tgatctatta
     2461 gaagccttta aatcccctat tggctgggta gctgtttttt gcggtgggct agtggctgtc
     2521 ttatcttcca agggtgtagg attgattgct tctgatccac aaatcactgt tgcacttgtt
     2581 ttcggcacga ttttaggtgt tgtcttctta agaggaatcg ccgccggacc ggtcattgct
     2641 tctggtatca cttactgtat cttacaagta tttgctttag gaaaatagaa caaattttgt
     2701 ctattttagg agattcttaa cacccccaat ttactgatcc gtgatatact gacaacgagg
     2761 tgatacaatg ttcgaaaaat taaagtattt cataggaaca atcggctggt ttcttctagc
     2821 ggtacttttg attctgggct tagcttatct attatttcct gatttgatac aaaatgtgtt
     2881 tgatactgct ttttgattga cctattaaaa cgatataggg gatgctcctg cgatgcaggg
     2941 gcttttttta atcaatgttt tgtaataatt taggccgtgc caatgagact tcctatagaa
     3001 ttcgatgctc ttttttcttt ttggctcttc tgctcttttt tgttatacta gattacagtg
     3061 gacaaaagaa agaatgatcg catccacttc aacgaatatc aaaatggaag aattagtgcc
     3121 tgttttcagg ttactttgaa aagaaaggaa gacgcttgtg gaaacttttg aaggtctttt
     3181 aattgatacg tacgttttac aagaacagcc aatgatgctg actgggatca acttgcagct
     3241 tgaggaccgt gtgatcaatg gcctgcttaa agatcgctat ttggcaaaat atatcttacg
     3301 tttgccccta aataaatatg tgctggaagt aagtggcaaa tggaaccgtc ggaaccagtt
     3361 ggtcataaaa aaaatgttga taaaaaatat cgatgaatac attaaaataa tcggtacacc
     3421 agaatagcct ttactgatca aaggatactg gttataaaat aactgcagtt tcttaataga
     3481 aaaacaaaga acaacaggga atcgtatccc ttttcaaaaa aaactttgca aacggttctc
     3541 tttgttatat aataacttag taccaaacat tcccattcaa actttgttta aatagataaa
     3601 taaaaatcta gtttggtttg acgctattgc ctcccacaat agcgtctttt tgattttttt
     3661 cagcttgtac tatccgtttt tactgaaaaa aaatcctaat aaaagactgt taaggaaaaa
     3721 aacttgtcat gttcatattg caaagcacgc tgcctttagg tataataatg aatgttgaat
     3781 tatgcggtaa cacgcgtaag aacttaagtt atcggaggtg aaactaatgc caaacattga
     3841 atctgcaatc aaacgtgttc gtacaagcga aaacgctaat gtgaaaaatt catctcaaac
     3901 aagcgctatg cgtactgcaa tcaaaaaatt tgaagatgct gtagcttcag gtgctgacaa
     3961 cgtagatgcg ttatataaag aagctgttaa agctattgat atggctgaat caaaaggtct
     4021 aatccacaaa aacaaagcta accgcgacaa atctcgttta agcaaaaaaa tcgctaaata
     4081 aaagaatcgg cttcggccga tttttttatt tgtcaaaaaa aggacaaaaa caaacgacct
     4141 tcccgcatgc tttgccctcg tctttcttct tatcgatttc ttaacaactg ccctgtgcgc
     4201 aaaacgaata gttgaaaact taattctttt tcgatttgtc ccgttttgat ttgataatcc
     4261 atctcaacta attgatcaaa caactgtacc agcaaatgtt catcgaattt ttttacttcc
     4321 tgcatggcaa gttttacacg atatggatgg attttcaatg tttcagcaat atttgcctgc
     4381 tgataaccaa tctttacaag aaattttgtc tgtaaaaata aacgtaattg agaaagcaaa
     4441 atcgcattaa ttttgattgt ttcttcccct tgcataacta aatcttcgta taaacgcaaa
     4501 gcttgttccg tcttcccttt taaaatatat tgggtcatgt caaaaatatt atgttctaac
     4561 gttttaggaa ctaattcttc tacttctctt accgtgattt gctgttgatt ttgtccataa
     4621 aggaccaatt tctgtaactc tctcatcatt ttggataaat ctaagtcggt caacctcaag
     4681 aataaatcaa ttgcacttcg ttccattttg attgaggaat tctctaaggt attcatcaga
     4741 tattgacgta cttctttttc attcaactgt tggacattga tgataattgc tgtttttttc
     4801 aattgtttcg ttactttttt ccgttcatcc aacttttcat atggcgcaaa gaatactaat
     4861 acagtcgatt ctaatggttc tttcaaataa gccgtcaata aatcagtatt ttgttcgatt
     4921 ccattgttta ctttttcacc tgttaaaaaa taaggattct ctacgaatac taaccgttga
     4981 tctccgaaaa acggcaacgt ctctgcttca gctactacct catctaaact tgcattttcc
     5041 atgtcaaaac ttaaaaaatt taagtcatct tcgccctcta attggatttt tttgacaatt
     5101 tctgtccgaa cttgatcttg gagaaacttt tctgttccta aaatcaagta acaagatgca
     5161 agtttttctt tacgtatttt ttgtaactct gcttgtatac tcaaattact caccactttc
     5221 aaaaattatc ctaccatgag cagatgagaa ttactagtct atcattgttt ccactagccc
     5281 atgaggatga aaaactggat gccagcgata ggtgatcatt ccttgttgat ccgttcgcca
     5341 tgtttttatc tgattttctt ctagtgtctc caatacttcg ctgtgcgggt gaccataacg
     5401 attatttacg ccgcaggata taatcccatg ttttgcttca atatgggaaa gaaaatctgt
     5461 gttacttgac gtacggctac cgtgatgacc taatttgact acatcggctt tcagattagg
     5521 ataaatcaaa agcaattgtt tttccattgc ttgattcaaa tctcctaaaa ataaaaagcg
     5581 cctattgtta tattccatcc atagaccaat cgaatcttca tttcttccct ctcccttttc
     5641 aaaaggagtc aatacataaa gatcataata accgctgatc ctgtccccat gtttgatttc
     5701 aatgatctca gtaccatttt tttgagcttc agataaatgc ctttgtaatg gctcatttcg
     5761 tccagcgccc caaccgatat aaattttttt gatagtaaaa tgctgagata acgtgggtaa
     5821 ttctcccaca tgatccgtat cgtcgtgtgt caagatgagt ttatcgatta cacttatccc
     5881 ctgacctttt agatatggaa cgatatttct ttcagcagga ggacgtatca taccttgcgc
     5941 ccacttttct ctttcaaagt gtaactgacc tccagtatcg atcaaaatcg tttcttttcg
     6001 aaaaggactt tgcaaaaaga tactgtctcc ttgaccgaca tcgataaaag cgatcgttga
     6061 agaaaagcgt aaaaatggca aagtcaaaaa aataaaagaa aaaagaccaa cataaaacaa
     6121 gtgtttcaca ggttgttctt tcaacctatc catacaaatc agtaatccga aaatatacat
     6181 cccagatagc acaaggggca aacgtcctat aatgagagac gaccctgaag aaaaatcgat
     6241 tattcgctca aataagatta atccctcttc tatcagcaaa agtaaagttt ccggcagcca
     6301 gcgattgaag agtgtgagaa ataacacacc aggaagaaaa agaaacttga ataccggtgc
     6361 aatcagtaat gtaaaaagac tacctaaaat aggccactca taaaaatgcc atacaatcaa
     6421 cggcatcgtg atcatagtaa acaggacgtg agaggccgtt tttttcaacc aatctccatc
     6481 aaaagaaatc aggataaaca aaaaactcat gaaaaaacta agctgtccac ccgcatgaaa
     6541 aagaaatcga ggatcaaata gtaaaagaag ccaaaagaca actgcaaata agtccatggt
     6601 ggaataatgc ttattcagca atttgactag caatcgcaat aaaaacagca aactagcacg
     6661 caaaacagaa atagaacttc ctgcaataga aacataacag atagttagta gactcaacgg
     6721 aataatactg acgtgtagtg gccatccgat acgtcgaaat aaataataca gccaccctaa
     6781 ataaaattgg atgtgtagac ccgatagact gaacagatgc agcaaaccac tctccctaaa
     6841 aatttcttct ggttctctga atcctgcatc tttatagccc cataataaag aattaatata
     6901 tacacttgtt ttttgcggaa attcagattg gacaaaggag ataaaagatc ctctttttct
     6961 ctgaagccag tttcgccagt gattttgcat ggtgatctgt tcaatctttt gaatttggaa
     7021 ccgtccagaa attctatggt tcctgaagta agcttcttgg tcaaaattat aaagattccg
     7081 cttttttgct ggagagatga actcgccttc agcagtcaat tctattccct ttcctcggaa
     7141 gttaagccat gttttcaatt ctgcttctgt gtttacttga taagaaagct ggagcttttg
     7201 tttattctct gataaccttc cttccattgt caaaaaatta ccatttgcac gaatcgtatc
     7261 tgtaaataca gtaatttgcg cagatgttgt tttttcctct aaaaatgaat actcttgtag
     7321 cctataagaa atggataccg ttatgctaca caaaatacac aagtacgcga taaaacgtcc
     7381 ttcttttagc gaaaaccata aaaaaattcc ggcaagaagc aaacgataac cccaattgtc
     7441 agtgtaatac aacaaacaga taatcatcga cgtaattgcc ggaaaaatca accgatttcg
     7501 aacatgcctt tctaaaacgc tagacatgtt ttcctgttaa ttcattttga agttttgaaa
     7561 aatgctgtga gtctaaagca acttggtgta cagatacacc taattgttcg atcaacgctt
     7621 gggcataagg atcatttcga tagtcatgga gataattgat ccctttcaca ccagcttgca
     7681 acaaagcctt tgtacaggcc aaacatggaa aatgtgtgac atagatttct gaaccttccg
     7741 tcgggatacc aagttttgca cactgtagga gtgcattcat ctctgcatgg atcgttcgca
     7801 agcagtgtcc atccacgacg taacaccctt catctatgca gtggacatct ccactgactg
     7861 ctccattata gccgccagca atgatccttt tttctcgaac gagtgtcgct cctacttcta
     7921 aacgtgtaca cgtactacgt aaagataaca gtactgcctg agccataaag tactggtccc
     7981 aaggaatgcg ttcattatgt gacttatcag taactttttc tttattcgta tcgcatgctt
     8041 ttgaagattt ttctaacatg ttttatattc ctccgttttt tcaagttgat taaaaagtac
     8101 tcattgctta aacaaaatat tctttaaata taacgaatca tcctgtctga caaaagatcc
     8161 tacccttcac tgccgaaatg aagtccgtca tccattagcc cattataaaa attcttcgaa
     8221 tccatttgtt caagaaattc cgtataaaaa tcgatacttc acaatcatgg cgttttgcta
     8281 cttctgtgag aacagagata tattctttga tcaacttgtt attccgatat ttttgttttg
     8341 tctcatcgac tgctggtggc gtgataaaga aagcatgatc agaactatag tcttttttca
     8401 gctgaactat catccactgc agattttttg gtacgtttct ttagaaagtt gtttatgctc
     8461 tgctagatca ttcgtcccaa tgagaataaa tactttatct gctgtatagc taagtacatc
     8521 tttggcaaag cgttctcgta gagcttgact gttattgccg ctgacagccg tattcataat
     8581 atgaatccca gatattttat tttggagata gacattcagc attggttttt ttaatgtttc
     8641 tttgcgaaca atcaagctat ttcctatcca aaaatactac ctcctcgtcc tgccaagctc
     8701 ttagaattgg attattctta gtatccatta gcgtccattt attgctcgtg ccagtcgatt
     8761 ttgaatcatt ctatttaatt gtaacgaagt tttttagttt ttcaactgtc ttttcaccga
     8821 taccagaaat ctcctgcagg tcttccactt tctggaagga tccgttttct tctctaaatc
     8881 gaatgatttc ttgcgctttg actgatccga tcccaggaat ctgctgaagt tctgatggag
     8941 tggctgtatt gatatttatt ttgggaggct cggaatctgc tgtaggctgt tttccttccg
     9001 aggatgccaa ctgatcaaga tgttctggca gctcttcacc tacagcaggg atatatagga
     9061 cttgctgatc ttctatcttc gcagaaaagt tgattcgatc gctatccgct tcttctaata
     9121 atccacctgc acgctttagt acatcataga cccgttcttc tgaagaaaag gaatagatac
     9181 caggctcctt cacacttcct ttgatatcgg tgtacatgac ctttggacga gcatcctctt
     9241 ctacatcgtt tccttcagct gatggatctt ctccactgga actaaacgaa gaaagtattt
     9301 cttttgcagc agtctcttct gtttctctat aaaatagcaa atttgataat aaaaaaccac
     9361 ttgcgataca aaagataaga actatcatag acatccaaat gatcaatttg ggtgaagcta
     9421 gtattttctt gctgattttt tctatcattg ttcttttttc aaagaatgac cagtaaaaat
     9481 cattcattac tcctttcatt taaaattgaa gaaacagtga gctctggcaa aatcgtccct
     9541 tcaacatcta attacgtcaa ttttttcttt tgtttttatt tttatcgtga agatttttga
//
LOCUS       DAZULY010000113         9537 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000113 DAZULY010000000
VERSION     DAZULY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.114"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1016
                     /locus_tag="ACG81A_001740"
     CDS             147..1016
                     /locus_tag="ACG81A_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5015101.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="ACG81A_001741"
     CDS             1225..2250
                     /locus_tag="ACG81A_001741"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFS5015102.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="ACG81A_001742"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="ACG81A_001742"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFS5015103.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="ACG81A_001743"
     CDS             3333..5036
                     /locus_tag="ACG81A_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5015104.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="ACG81A_001744"
     CDS             5038..7071
                     /locus_tag="ACG81A_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFS5015105.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="ACG81A_001745"
     CDS             7082..7522
                     /locus_tag="ACG81A_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5015106.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="ACG81A_001746"
     CDS             complement(7722..8849)
                     /locus_tag="ACG81A_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5015107.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="ACG81A_001747"
     CDS             complement(8852..9529)
                     /locus_tag="ACG81A_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5015108.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
BASE COUNT         3240 a         1648 c         1952 g         2697 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggc
//
LOCUS       DAZULY010000114         9485 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000114 DAZULY010000000
VERSION     DAZULY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.115"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..916
                     /gene="gnd"
                     /locus_tag="ACG81A_001748"
     CDS             14..916
                     /gene="gnd"
                     /locus_tag="ACG81A_001748"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFS5015109.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            918..1919
                     /locus_tag="ACG81A_001749"
     CDS             918..1919
                     /locus_tag="ACG81A_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFS5015110.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1916..2200
                     /locus_tag="ACG81A_001750"
     CDS             1916..2200
                     /locus_tag="ACG81A_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFS5015111.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2233..3030
                     /locus_tag="ACG81A_001751"
     CDS             2233..3030
                     /locus_tag="ACG81A_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFS5015112.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3044..3877
                     /locus_tag="ACG81A_001752"
     CDS             3044..3877
                     /locus_tag="ACG81A_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5015113.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3895..4752
                     /locus_tag="ACG81A_001753"
     CDS             3895..4752
                     /locus_tag="ACG81A_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5015114.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4774..5283
                     /locus_tag="ACG81A_001754"
     CDS             4774..5283
                     /locus_tag="ACG81A_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS5015115.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5320..5721
                     /locus_tag="ACG81A_001755"
     CDS             5320..5721
                     /locus_tag="ACG81A_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015116.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5833..6186
                     /locus_tag="ACG81A_001756"
     CDS             5833..6186
                     /locus_tag="ACG81A_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015117.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6220..6663
                     /locus_tag="ACG81A_001757"
     CDS             6220..6663
                     /locus_tag="ACG81A_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5015118.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6680..7765
                     /gene="uxuA"
                     /locus_tag="ACG81A_001758"
     CDS             6680..7765
                     /gene="uxuA"
                     /locus_tag="ACG81A_001758"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS5015119.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7758..8471
                     /locus_tag="ACG81A_001759"
     CDS             7758..8471
                     /locus_tag="ACG81A_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5015120.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(8678..8827)
                     /locus_tag="ACG81A_001760"
     CDS             complement(8678..8827)
                     /locus_tag="ACG81A_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015121.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            9223..9423
                     /locus_tag="ACG81A_001761"
     CDS             9223..9423
                     /locus_tag="ACG81A_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5015122.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3181 a         1496 c         2109 g         2699 t
ORIGIN      
        1 tggaggcgct atcatggaaa ttggtgtaat tggtttggga aaaatgggat tgaatttagc
       61 tttaaactta aaagagcacc atcacaaagt acaaggactc gacatttcaa gtacagctgt
      121 tagtcaggca agagaaaatg acattcctgc ttatcaagag atctctgaat ttctagctac
      181 ttttgagcag aaaaaaatca tctggttaat gcttcctgct ggagagataa ccgaaaacat
      241 gttaagaaaa ctttttccaa aactcggacc aggagacatt attattgaag gcggaaactc
      301 ttattataaa gattcaatcc gtcgagcaga agagtttcag aaaaatgaaa tcggttactt
      361 ggattgtggg acttctggag gaatcgaagg agcacgacat caagcctgct taatgattgg
      421 aggagataaa gaaacttttc tatccgtgga acaattattc aaagatgtag caattgaaag
      481 aggatatctg tatgctggtg catccggaag cggacacttc ttgaaaatga tccataatgg
      541 aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc caacttgtac aaaaaagtga
      601 gtatgctttt gacttggaac aagtcgcacg tgtttggaat catggttcgg tgattcgcgg
      661 ctggctgatg gaaatcatgg aagatcagtt aaacgaacat cccaatttaa gtaattatcg
      721 gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt gaaacagcgc tagaaatggc
      781 tgttccagtt ccagtaattg caactagttt gttcatgagg aatctttcac aggaagaaga
      841 tagtttttca gcaaaagtag tttcagcact aagaaatggg tttggcggac atgaaatagt
      901 tgaaaagggt gagtaaaatg ccagattatc atatagcaat cgttaattcc agcagtttcg
      961 gaagagtctt tccacaccac ttggaaagat taaagtcagt tggagacgtt catttcttta
     1021 ctgttgatca agagattcca ggaaaagaac tagcggagtt acttaaagga ttcaatatca
     1081 ttattgcaag tgttacgcca ttttttcgac gagatttttt tgaaaacaaa gatgaactgc
     1141 tattgatttc ccgacatggt attggttaca acaatattga tttaaaagca gcaaaagaac
     1201 accagacaat cgtatctatc atacctgctt tagttgaacg aaatgctgta gcagaaaata
     1261 atataactaa tttgttgtcc atgcttcgac gcacgacaca agcacaagca agagtcaagg
     1321 acgatcgatg ggaagatcgg gcggagttcg tcggtaggac gctgtttaac aaaacagttg
     1381 gtgtgattgg tgcaggaaat accggaagcg gagtaatcga aattttgcgt aacggttttc
     1441 gctgtgatgt tctagcttat gatccttaca aatcagattt atacatcgaa agctttggtg
     1501 caaaaaaagt agaacttcaa gaattactgg aacaatcaga tatcatttgt ttatgtgcga
     1561 atttgacaga agagaactat cacatgattt ctgataaaga agtcgatcag atgaaagaaa
     1621 atgtttatct atctaatagt gcaagaggcg ctctagttga tgaagaagcg atgattaatg
     1681 gtctgaaaag cggaaagatc gcaggatatg ccacagatgt tttagaagaa gaaccgggac
     1741 gtcaagatca tccgtattta gcgtttgaac aagtagtgat tacgccacat atatcggcct
     1801 acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt cagagatgtc gaacagatca
     1861 gtcagcacct tcttcctgaa cgttctgtcc agacaaagag caactatata aaaggatgat
     1921 gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct caattttatt tatatgaaga
     1981 aggtggtatt tcaaaagttt ctgatctctt gaaatcatac ggcgcgaaaa gagtactggt
     2041 cacccacgga acggtatcat gggaaaaggc gctacctaaa ttagtatttt tgaatgacga
     2101 aacgattcaa tttttttatc atcgttattc tggagaatgt agttatgctg aagccagacg
     2161 aatagctaca atcatcaaaa aaatgaaatc gatttcgtag ttggcgttgg cggaggaaaa
     2221 ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa tcgatttcgg agtcatccct
     2281 actttagcta gtaattgtgc tccatggaca cctcttgctg tcatgtataa ggagaatgga
     2341 caagcagaag ggaaaacaga acacttttac agacaagctg ctttttttat aacagatccc
     2401 caattagtga ttgattcccc tgttccatat ttcattgccg ggttagcaga tacactagca
     2461 aaatggtatg aatctgagct catcttagaa caagattttt tgcaagatga atcatttttg
     2521 aagctagcac aagatactgc gaagatctgc aaagaagaga ttttgaatca ttctgctaaa
     2581 gcaatccaag atatggcaca aagaaaactg acatcggagt tcaaacagct ttcagagatt
     2641 atctttgctg ttgctggttt agttggagga ttaggagata aatacgcaag aaatgcggca
     2701 gcacatgcta tgcatgacgc tatcagtaaa tatattccgg aaagccatcg ttttctacat
     2761 ggagaaaaag tagcgtatgg gattttctat caactggcac tagaaggtcg ctgggaaaaa
     2821 atagacgagt tatatccttt gtatcgggaa ttagaactgc cggtttctct agaacaaatg
     2881 ggattatttc cgaaagataa acagataata gataaaatgg ctgaatttat tgattcgaaa
     2941 gagaaagtgc atttgattcc tatagaggta acacagaaat cactgataca gtcaatgatg
     3001 gaactggaag gttatatcag aaagatctga aggaggagga cacatggaaa cattaagtgt
     3061 agtacaaagt ttgttgattg ctttatgggt agccgcggtt atgtcccgct ggttaggtgg
     3121 aggagccacg ttgaccttac gtttttctcc tttgatgact ggattagtcg tcgggttgat
     3181 catgggggaa gttgcacaag cgatgattgt tacagccgct ttgcaaatga tttatatggg
     3241 gggtttttct ccaggaggat caatgccagc tgaaccatct attgcagcag ctatcgcagt
     3301 accagtcgca ttattagggg atctgtcgcc agaagcagcg attgccgttg cggtgccagt
     3361 cggattgctc ggaagttact tgtatcagtt tcgttttttc atcaatactt ttttaggtaa
     3421 atatacagac aaagctgtag aagatttgaa tacaaaagct atggcccgat caattattct
     3481 ttatccaacg gttgcctcat ttttattatt cgttccgctt gttttcattg ctttatattt
     3541 aggggcgcca ttgatcgctg atgtaatcaa agctttagag ggaacagtcg ttatccacat
     3601 tcttgaagta gtaggtggag ggttagcggc aatcggtatc gcgacaactg tatacgttat
     3661 cggtcgaaaa gattatctgg tctttttctt ccttgcttat tttatgagtg ttgttttgaa
     3721 atctttagat attacgatgg ttacttatgc catttttggg attattatcg ctttgatctt
     3781 tgtccagtcg caaaaaggga aacaagtaca gcctgccaca agcaataaca attcagcatc
     3841 ttttgatgac gaagatgact atgatgacgg attttaattt aggaggaatg ggaaatggct
     3901 gaaataatgg ataatcaaac aggtgctcct gaagaaatca cgaaaaaaga tgtaacaaaa
     3961 gcctatctga gatggcattt tgcaaacgaa attcctcact cttttgaacg ttacttggcg
     4021 ccatcactac tatacggaat gatgccaatc ttgaaaaaac tttataaaga tgaagactct
     4081 ttaaaagcag catataaacg gcagttactg ttcttcaaca ctcaattaag ctggggtggc
     4141 ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa gagcagaaga agagtatgaa
     4201 gaaaaagaga tcttgatgca ggatgacttg atgtataaca cgaaagcggg attgatgggg
     4261 gcactagcag gaatcggaga tgcaatcgat tcaggaaccg tccaatatat ttttatcgca
     4321 atcgcggtac cttgggcaca acagggaagt cctttaggcg cactttttcc ttttatctgc
     4381 tttgggttgt atcaggtttt actaggggta tttttcgcaa gacaagcttt cactatgggc
     4441 cgtaatgcta caggcctaat gcaaaacact ggtgttcaga cagcgattga aatgctatca
     4501 gttttagggt tgtttatgat gggggtgctt gcgggaaact atgtaaaagt agaatcttca
     4561 ttgaaatttg cgatatccgg acgtgagttt gttatccagg aaattcttga tcaaatcgtg
     4621 ccagggatat tacctttagc tgtcgtaatg ggtgtgtact tgttctatac gaaaaaagga
     4681 ttgaaagtca cacaagcatt attatggctg actggaatat taattgtgct agcaggaatc
     4741 ggtattttat aaaaagaagg gaacgtgaat aagatgggtg tagtcaattt agcgcgtgtg
     4801 gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat ctcaaaaaag tggagtgaat
     4861 tccatttttg ttgtagacga agttgtagca aaagataaat ttatgagaga tctttacaaa
     4921 aatgcaggaa gccgtacagg acaaaaaaca attgtcatta caccagagaa agctaaattc
     4981 tattgggatg aatatcaatt taaagaatac aattgtattt tgatcgcaaa aacagtccaa
     5041 acgatttacg acttagtaaa acacggaatc cccatggaag aattgaatat tggagggatt
     5101 gctcaaaaaa atcctgatac agatctttta gtgacgaaat ctgtttattt aaataaatca
     5161 gatgctgaaa aattgaaaga aatgaatgaa caatatggcg taaagaatat ttattttcaa
     5221 gccactccat ctgcacctaa aacaggatta aaagatgtat tgaacaagtt tgatttgtca
     5281 taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa tgaagaagaa cgagctgttt
     5341 cgtgattggg aatttcgtta tcgctatatt taccgtaagc gccgtacaaa aaagagtaaa
     5401 cagcgttttc tttcagcatt agtatcagat atttacagta tgagaacaga tgtgacagtc
     5461 attgcttacg atacgctagc ctaccgatca aaaaatattt atgttggaga tatcgaaaaa
     5521 gcagagaaag ttatctgtac ttattacgat acacccgttc atgcattagg aagttatttc
     5581 atgtttgatt ggaaagacca aaggaaaaaa acaatctact ctatattatt gtccttcatt
     5641 ttgttgttca gtttaggttg gtggggaatg atgatatata acaaaaatcc tcatcacgta
     5701 ttcgatctgc tatccgtata aacaggtatc actgtactag catttgggag ctactttttc
     5761 ttattgggaa aagcagctcg cggatggcct agcagacaaa cttttattcg aaatacttca
     5821 tccatattaa cgatgctaga aatgatccgt acgattgatg atccgagtgt agcgtatgcg
     5881 tttgttgatg aaggctgtta tggagaaaag ggcttagaca gcgtgagatc gggtatgaaa
     5941 aaagaggcta ttctttttta tctagactct gttggggcag atacaccgct acagttttcg
     6001 ggaaattatt tttcaaataa agaacagtgg ctgaaacaag ttgataagtt gaaagaaaaa
     6061 aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc agttcttcta tttgaccaaa
     6121 acagatttac gagggaaaac atttaattgg caaaatgcca atcagattat tgctcttttt
     6181 cgataagtaa aaagtgcaaa acaaggagtg agaagactaa tgttggaaat catgatcgtt
     6241 acacatggta agttgagcga aggactgaaa gatgcggcgg aagtaattgt cgggacatcg
     6301 agccatattg aaacaatgtg cttagagtct ggggaggaca tccaaacatt cggagagaag
     6361 gtcaaagaaa aattaactat gatcaacaca gcagaaccca tgctagtatt tgttgatcta
     6421 attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca atgaactgac tgaagaaaag
     6481 aaagcagcca cttatgtcat agggggcgtg aatctgccaa tgttgttaga agcaatcaat
     6541 catcagatgc ttcacacacc gatagaagaa gcggtggaac aaattgtcaa acaaggaaaa
     6601 gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa aattagatga ggacgatttt
     6661 tagaaggaag gctgaaaaga tgaagtgggg atttagatgg tatggtgaaa aaggggattc
     6721 gatcccatta aaatatgttc gccaaatacc gggaataaat gggataattg gaactttgct
     6781 gactaaaatg cctggagatg tttgggagat ttcagaaatt gaggagttga aagcatctgt
     6841 tgagagccaa ggactggaac tgcttggaat tgaaagtgta gcgatccacg atgcaatcaa
     6901 ggcagggaca gatgaacgag attactatat tgataactat atacgaacaa tcaaaaactt
     6961 gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa ccaatttttg gatgggcgaa
     7021 aactgatttg tttaatgaaa acaacgatgg aagctattct cttttatacg atcagaaaat
     7081 cgtcgatcag atggagccag gagaaatgta tcaattgata cacagccagt caaagggatt
     7141 caaattgccg ggttgggaag aagaacggtt gaaaaaattc gctcagctgg aaaaactgta
     7201 tgaaggaatg accgaagaag aattatttga aaatctgact tattttttag aaaaagtgat
     7261 tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat ccagatgatc cgccatggga
     7321 actttttggt tttcctagag tgacgaaaaa tcttggcgac ttgaagaaaa ttctaaatgc
     7381 agtagattct ccatataatg gactaacgct gtgcacagga tctttagggg caaatccgga
     7441 aaacgattta gttgaaatca tccatgaggt aggttcgcgc atccattttg tccacttcag
     7501 aaacgtggag tttttaggag aaagaaagtt caaagaaacg gcacatccct ctacagaagg
     7561 ctcccttgat atgtatcata ttatgaaagc gttggtgcaa gaagggttcg acggtcctat
     7621 acgccctgat catggtagaa ccgtgtgggg tgaagtggct atgcctggat atggcctgta
     7681 tgatcgtgca atgggactca cttacatgca aggtctatat gaggcgataa gtaaggaaaa
     7741 accactggga gagcattatg aataaaatca agctcttcat ctttgatatg gatggccttt
     7801 tattagaaac tggacgttta gcttatcgtg cttatgtaaa atcagcacaa aagtatgatt
     7861 atgaaatgcg taaggaagtc tattatttac taactggcca aacagaaatg gcgataagag
     7921 aacaaatggg cattttgtac ggagaagatg ttccatatat caaatggcga gaagcaatca
     7981 atcaatataa agaaaaaatc gtgaaagaag ataagagagt atatacgaaa aaaggagcag
     8041 aagaaatttt atcttttgca aaagaaagag gaatccatac aatcgtggct tcgtctaata
     8101 cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct atatgccttg tttgatcaaa
     8161 tcatttcagg ggatgatgta aaaaaaggaa agccggaacc agagatcttt ttgaaagctt
     8221 gttcaaaaat gaatattcct ccaagtgaag cacttgtatt tgaagactcg attgcaggga
     8281 tcgaagcagc aaggcgagcg ggcattttgt cttttctcat caaagatcat ttgatagggt
     8341 tgcccgatca taatgggaag cataagttga aagtagatgt aagtttatat gaatccaata
     8401 aagcagacta tgaattttat gacctgcatc aagcaaaacg atttttagaa agtaaagggt
     8461 tgtatctcta ataaagagga ctattttcta gtcctcttta tttattgtcc tgcacgactg
     8521 agggaacaga tgaaaaagaa gtaatttttt attttgaagt atctacctta tttgtagtga
     8581 acaatttaga acaaacacaa aacgcatcta ctgttatcta ccagtagatg cgtttaaaga
     8641 gaagaacgag aatcgtccat atgagggttt ccactcctta tgcagatttt tttaattctt
     8701 gcactgcttg ttcgatcgta acacctgtcg ctacatgatt ggtattttct ttgtcaaaaa
     8761 cgacaaatag attcaaatct gtatccaagg ttactcgata ttgtatatct ttgttataga
     8821 atgtcatagc cattctcctt tctttaaata cttgctgaaa ggcaacgaac gcgttgataa
     8881 cgaaattcca tttataagta taccaccaat tgaaaggttt tcatagttaa gaaattttaa
     8941 agtcaaaagt tgcagataaa agcaaataaa caagaaaaca aaaagtgaat caatcgcggt
     9001 tattcaactt tttcttcgat tcgaagacaa tagaagacaa tgaattttat atttcattgc
     9061 attatccttg tgttattaga aaaagcatga tatattaaat gagtaatcta ttttgaaacg
     9121 taatttgagc gatatatcta catagataaa actccttttg cctatcaaat atttattgca
     9181 acgaaaaaac gtacattgat acgtatcagg aggaatttat ttatgaataa cggtacagta
     9241 aaatggttca acgcagacaa aggttttggc tttatcactg gtgaagatgg aaatgatgta
     9301 tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa ctttagaaga aggccaagca
     9361 gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag caacaaacat tgttaaagct
     9421 taataaaaat tgacttttaa ccgtcttttc gaaagaaaag acggtttttt tgtacgaaaa
     9481 aattc
//
LOCUS       DAZULY010000115         9421 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000115 DAZULY010000000
VERSION     DAZULY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.116"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            115..864
                     /locus_tag="ACG81A_001762"
     CDS             115..864
                     /locus_tag="ACG81A_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFS5015123.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            855..1640
                     /locus_tag="ACG81A_001763"
     CDS             855..1640
                     /locus_tag="ACG81A_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5015124.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            1609..2325
                     /locus_tag="ACG81A_001764"
     CDS             1609..2325
                     /locus_tag="ACG81A_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS5015125.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(2427..3446)
                     /locus_tag="ACG81A_001765"
     CDS             complement(2427..3446)
                     /locus_tag="ACG81A_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFS5015126.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(3465..4466)
                     /locus_tag="ACG81A_001766"
     CDS             complement(3465..4466)
                     /locus_tag="ACG81A_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFS5015127.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            complement(4743..5891)
                     /gene="nagA"
                     /locus_tag="ACG81A_001767"
     CDS             complement(4743..5891)
                     /gene="nagA"
                     /locus_tag="ACG81A_001767"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFS5015128.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(5967..6803)
                     /locus_tag="ACG81A_001768"
     CDS             complement(5967..6803)
                     /locus_tag="ACG81A_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5015129.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(6987..8204)
                     /locus_tag="ACG81A_001769"
     CDS             complement(6987..8204)
                     /locus_tag="ACG81A_001769"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFS5015130.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(8353..8514)
                     /locus_tag="ACG81A_001770"
     CDS             complement(8353..8514)
                     /locus_tag="ACG81A_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015131.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            8587..8670
                     /locus_tag="ACG81A_001771"
     tRNA            8587..8670
                     /locus_tag="ACG81A_001771"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8621..8623,aa:Leu,seq:gag)
     gene            complement(8910..>9421)
                     /locus_tag="ACG81A_001772"
     CDS             complement(8910..>9421)
                     /locus_tag="ACG81A_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFS5015132.1"
                     /translation="YVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLN
                     NLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCS
                     RLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGIC
                     TFIITVINR"
BASE COUNT         2979 a         1907 c         1595 g         2940 t
ORIGIN      
        1 ttttttaaga aaaaaccatt aaagctccag aacacgatca tccttgtcgt ttgtttctcc
       61 acattcgttt ctttgattgt ttctgccata ttgatcagga attttattct ttcgatgaac
      121 cgatctagtg tgtcagataa aatctctagc attgctaaaa tcgttgccaa tgataaactc
      181 atccaaaaaa agctgcagac tggtgacccg acacatgcga ttcaaggaat aacaaatgaa
      241 atcatgattg catcgaattt agattttatc gttgtcatgg accctgaagg cattcgatat
      301 tctcacgcag acccaagtta catcggtctt cctttctcaa gtcatgaaga tgtgaaaaaa
      361 gtgattggag gacaagaatt tttctctgaa aaagaaggaa tgttagggtt cggcttccgc
      421 tatttcgtcc ctatttatta tgatcaagag attattggtg cagtttgtgt tggcttaaca
      481 aacaacacga ttgacaatca agtcaaagat ggacaaaaaa tgattgtttt ttcattgatt
      541 attggactta gcgtaggcat cataggcgcc gtttttcttg cccaaaaaat gaaaaaagta
      601 ctgcttggac ttgaaccaca agaaatagca gaacagttaa gtgaaaaaac gattatcgaa
      661 aatgaagtag atgaaggaat cttagccatc tcttctgata aaactatcct cttgatcaat
      721 acacctgcaa aaaacatttt ctaccaaaca gagcagctat tggatttcga agtaggaaaa
      781 aaatcagtga taatctatat gacgttttgt ttaaagagac gattgagcat caaacgaccg
      841 ttcggactca atccatgtat ttaaatggga tagaaactat ttctagcgtt acacctcttt
      901 ttagcaatga ccgtttttta ggagcagtcg caactttacg aaatcgatca gaagtaatga
      961 aattaatgga ggagcttggg gggacgaaac aatatataaa ctccatgaga gctcaaaccc
     1021 atgaattcat gaataaactt catgtcattt ctggtttgat tgatttgaag aagtatgaag
     1081 aagtcaccca ttttatccag cagctaaatc aaaattataa aaaagagatt ggccaagtct
     1141 caagtctgat caaagtacct gcaattgccg gttttttatt aggcaaaata aacgaagcca
     1201 aagaacaatc tgttgaaatg ctgttagaaa gcgattcttt tatccctaaa atggagatgg
     1261 atgactccat ccattcactt ttgcaagtgt tagggaactt acttgataat gcaaaagaag
     1321 ccgtgctaca aaaacatgaa agaaacggac aggtccggat tcatttaaac tatgacacag
     1381 aagctgaagt attcatcgtg accgtctgcg atggcggtgt cgggatatca gaagaaatct
     1441 cagaaaaaat atttacgcta ggtttttcga cgaagggaat caatcgtggg tttggattga
     1501 atctcatcca ttcaatcgtc aaacgccacg gtggattttt agaattttcc aaacaaacaa
     1561 atggcggtac aatcgtctat ctagaattac caaaaaagga agatcaaaat gatgacaaat
     1621 gtcctgataa tcgaagatga tccaatggtt gcagaattga atcgtacttt tgtggaaagc
     1681 atccctaata tgaacgtaat cgaaagtttc agaaatgtag aagaaggaaa aaaatatttc
     1741 agacaaaaaa acgcagatct gctgctgctt gatgtataca tgcctggcga aagtggtatc
     1801 cattttttat cttggcttag acagcaagga aatgatatcc cagtgatttt gatcactgct
     1861 gcagatgata tcgaaaccat aaagaatgtg atccgctacg gtgccttaga ttatttgatc
     1921 aaaccgttca ctttgcaacg ttttcagctg gcgatcgaaa agtatcttgc tttctctcag
     1981 acaatccaaa cgactgaaaa agccgatcag aaattgatcg atcaagtatt caaccacacc
     2041 actcccattc acgaaatacc atcattcatc gatttgccta aaggtctgtc aaaactgact
     2101 ttaaaaaaaa taagtgcatt tattctaaaa gaagatacta ttttttcaac aaaagaactg
     2161 gctcaaaaaa gctcactgtc acgtatatcc gtcaagaaat atttgaattt tttagtagaa
     2221 caagatatat tgaacgaaag aatcaaatat ttggaaatcg gccagccgct aacctgttat
     2281 gaattgacaa agaatgggcg gaaaaagctc acagattact gttgagtcat cgaattatcc
     2341 catacaaaat agtccaagta tacagaagta acaactgttt acttggacta tttttaaaca
     2401 ttttattgac gaatatccat gttttcttat cttaattgac gggctttagg aagagatacc
     2461 tcatttttcg tttttccagt ttctattatt tcacgaaagt tctcgaaact tgtcgaaatc
     2521 atatttgata atgcctcatc tgtatttgaa cctacatgcg gggttacaag aactctaggg
     2581 tacatttcta ctaattgctg gatagcaggg tctggaatcg tctcccatgg cttgaaggat
     2641 cggaaaaaaa cctctgtttc atttgcaaaa acatcggttg caaaaccagc taaatgattg
     2701 cttttcaaag ctcttaaaat agcttcatta tcttgaagct ctccacgggc tgtattaatc
     2761 aagatgctac cttttttcat ttgagctaaa aatccttcat tgatcatttg atcgttttca
     2821 cctgggatat gaggtacatg aatactgaca atatcgcttt tggctaataa ctcttcaagc
     2881 gagtggaacg tcaatacttt tttagcagct tctgtctgat gaatatcgta gccgatcact
     2941 ttggctccta gtcccttaaa caaaacagct tccgtcaacc ctatcctgcc tgttcctatg
     3001 atccctactg tgcaatttct gatttcacga ctaaacattt gctcatccac tcgaaaatct
     3061 ttgaaagatg ttcggatcgt cgtatacgca gtatttctca aaagcatcat ggcaaaagtc
     3121 aaagataact ctgctatcgc atttggcgaa tacgatggta cacgggctac tgtcatacca
     3181 aaatcagctg ccgcttgtag atcgatatga ttgactccca cagtccgtgt aaaaacataa
     3241 cgaatcccat attcctgcat ttttgcaaga ttggtctcat ctgctataca atttcccctc
     3301 aacaatacag catcatggtc ctttgctgac acgatattgt catgtgtcaa cagttcttca
     3361 taaagactga gatcataatg gtaaatattc aatttttcaa aaaaatctat ctcgttcgga
     3421 cgaacaccgt aacatgcgat tctcatactt tcttcctcca tttcttatga aaataaacct
     3481 atgccatgaa tcatttgtaa tacaacgatc catacaggtg tcagtaccac agcaaaaata
     3541 gttgacagta aggatgcatt ggaggctaaa agtgcctccc tatcaaaact gatagcatag
     3601 gctgctgcca ctgttgcagc tggtgtagcc atcatgataa cgattgttgc cagtgcttca
     3661 aaattgactg gtagaatatt cgttgccgtc aaaactgcta gaatcacgat attcaatgca
     3721 ggtacaaata ccactttgac tagactgtaa taccatgaag ttttttcaga tgcagctttt
     3781 ttgaagctta cttcacctaa tgttgcgccg attgctagcc acgccaatgg tgaacttaaa
     3841 ccagctaaat aatccatcgg ctgaaatagc cacggtgcag tctggtcaat tcgcaaaaac
     3901 gctacttggc ttatctctcc ttctgcacct gtaactgaca cttgtggcag atacccttgg
     3961 aacagccaaa tgaaaaggcc ggcaaaagta gcgataatga ttggattgac gaacatcgct
     4021 ttgatatttt tagcttccat cttcaagcca ctcattttta tgtatccata agaatacaaa
     4081 aagattcggt agccaatatt gaaaattgag gaatacatca cgccgacagg accataaatc
     4141 gcactgacga taggcgttcc gaaaaaggtc gtagaaccaa agatcgtcag gacacgcaac
     4201 gttgtttgct tatccccttt atagcttaag aaaaaaggtt ttgaaactag aataagaata
     4261 atataaataa ctaatcccca aatcaagaca ttgattcctt gcatcagtaa ttctgtattg
     4321 atatcttgca tgaaagctcg aaatgccagt gctggcaaag ccactgttaa gacgactttc
     4381 gatagaattt ttccaacaga ttcagaaaaa atcccctttt ttctacaaac aaaccctaat
     4441 aatataataa aaactgttga agtgattgca ctgacaatcg acatatctgt caaagtcgat
     4501 cgaaaaatgt caaataagtt catctttctc ctcctaatta tgttcattta ttcacaataa
     4561 aaaaagcttt tattttgcta atacgtatta acatttttaa ttttatcttt acattgttac
     4621 ttattgaaca ataggaaatt aaagaaactt ttgaaactta tgtaaagtga tttttactag
     4681 aacacaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta acttaaatta
     4741 tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt catatcaggg
     4801 ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt atcatcgatt
     4861 ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg tgttgctaat
     4921 ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc agctaagttg
     4981 ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc taaattgtaa
     5041 tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac atgatcatgt
     5101 cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc atcacaaatc
     5161 aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg ttcgcgatga
     5221 ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc ttcaacagct
     5281 ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac tccttcttca
     5341 gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc aatttttttg
     5401 attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg atccccgaaa
     5461 taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc aaaatagatt
     5521 cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc agctacatct
     5581 tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac tccacaagat
     5641 aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac atcatgattc
     5701 atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc gctttggtca
     5761 atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag tccatcggta
     5821 atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc agcatagata
     5881 aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc ttatttagag
     5941 aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt cacagcttgt
     6001 gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta tattaagctt
     6061 ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc ccaatagatt
     6121 ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa gaaatttcca
     6181 taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga ttgatgcagc
     6241 agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc cacaccatat
     6301 atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat tctttctttt
     6361 tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat attttgacgc
     6421 aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt tgcttgatac
     6481 agattttcag aaatatcaga agattttagt acttgttcta acttccgata atcacgtttt
     6541 tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt ttttcgagag
     6601 agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat catcaccaat
     6661 acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga acaaatatca
     6721 tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat gatcgttcca
     6781 aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt cgcattctat
     6841 tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta aataatatac
     6901 tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt ctgtggccct
     6961 cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag gaattctctc
     7021 attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat tcgaaaatga
     7081 tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg ttcatgtaaa
     7141 aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc aatttttggg
     7201 aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc attgattgca
     7261 ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag ctcatcgaca
     7321 ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt ggaacacaaa
     7381 cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga cttatctcca
     7441 cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga tagaccgttc
     7501 agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc tagaccatcc
     7561 atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg tcgagctact
     7621 tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt attaggatta
     7681 atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc aatatcagga
     7741 taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc taatgcaata
     7801 gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc attgtccagc
     7861 aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta gatatcattg
     7921 atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt tctagcagaa
     7981 aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat caaatcacga
     8041 atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt caattttaaa
     8101 atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc gcgaacatcg
     8161 taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta gccaatccct
     8221 catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa tatcatactc
     8281 catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa aacacaaaca
     8341 agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc aatccattgc
     8401 aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg caagtccaat
     8461 caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct ccatcttaat
     8521 aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt agtgtgttat
     8581 tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg tgagcgttag
     8641 ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat tttgcttcac
     8701 gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt atcctttaca
     8761 aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg gaaacaagct
     8821 actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa atccctcacc
     8881 taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat tataaaggta
     8941 caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac actcatgctt
     9001 ttcattagct ctttaactgc cattagtact aagatggcga taacaacata aagtatatag
     9061 tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa ccttgaacaa
     9121 aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc aaaacttaaa
     9181 taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc tatccaagta
     9241 cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt caggttacta
     9301 ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg acccattgta
     9361 ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat acgcacataa
     9421 t
//
LOCUS       DAZULY010000116         9405 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000116 DAZULY010000000
VERSION     DAZULY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.117"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..2606
                     /locus_tag="ACG81A_001773"
     CDS             135..2606
                     /locus_tag="ACG81A_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5015133.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2709..4445)
                     /locus_tag="ACG81A_001774"
     CDS             complement(2709..4445)
                     /locus_tag="ACG81A_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5015134.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4442..5146)
                     /locus_tag="ACG81A_001775"
     CDS             complement(4442..5146)
                     /locus_tag="ACG81A_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5015135.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5277..5780
                     /locus_tag="ACG81A_001776"
     CDS             5277..5780
                     /locus_tag="ACG81A_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFS5015136.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5740..6078
                     /locus_tag="ACG81A_001777"
     CDS             5740..6078
                     /locus_tag="ACG81A_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFS5015137.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6099..7517
                     /gene="ffh"
                     /locus_tag="ACG81A_001778"
     CDS             6099..7517
                     /gene="ffh"
                     /locus_tag="ACG81A_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFS5015138.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7629..8207
                     /locus_tag="ACG81A_001779"
     CDS             7629..8207
                     /locus_tag="ACG81A_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFS5015139.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8211..8732
                     /locus_tag="ACG81A_001780"
     CDS             8211..8732
                     /locus_tag="ACG81A_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFS5015140.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8811..9365)
                     /locus_tag="ACG81A_001781"
     CDS             complement(8811..9365)
                     /locus_tag="ACG81A_001781"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFS5015141.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
BASE COUNT         3041 a         1738 c         1958 g         2668 t
ORIGIN      
        1 cctttttttt tgctatatga caggaatgaa aggtgtttgc aagcttaatg tgaggaacac
       61 tttccatatc tgttctatac tggataggag aagtttggaa atatccagaa aacgaaatca
      121 atcgaggtag ccacatgaag aataaatgga taaatcgaag taaaaaaaag aaagtaacac
      181 aaagatcatc agataaagtc atccgtcttt ttccatcgta taaagaagga ttaacggcag
      241 atcaagtcaa ggaacgaata gaaaaaggtg cagcaaataa ttcagtcgat cccacattca
      301 aaacaaatca gcaaatcgta ttggaaaaca tatttacgta tttcaatttg atctttctta
      361 tcttagccat tttgctttgt ttagtagaat cctataagaa cttgactttt ttaccagtta
      421 tcatagccaa tacaggaatc gccatttttc aggaaattcg atccaaaaag atcttggacg
      481 aactaaatgt tttaaataca gcaaaagtca atgtcatacg agacgggata gaacaaacaa
      541 tagatataga agaccttgta atagatgaca ttgttatttt agagactgga tatcaaattc
      601 cagcagatgc agaggtcgtg gaagggaaac tgcaagttaa tgaggcttta ttgacaggcg
      661 aagcagatga aataaccaaa gaaataggtg atccattaat gtccggcagt ttcattgttt
      721 ccggaaaagc ttacgcccga ttagacaaag ttggagcaga ttcgtatatt tcccagctga
      781 cagtcaaagc caagacaatg ggagaaggag aacagtcgga gatgattgct tctcttaatc
      841 aattgatcaa atggattggg atcatcatta ttcctatcgg tatcctgctt ttttctcaaa
      901 gttacttttt taatggaaac acgatcaaag aaagtgtcgt tgcaatggaa gcagcattga
      961 tcggtatgat acctgaagga ctgtacttgc tgacgacgat tgcattagca ttaagtgcga
     1021 ctaaactagc aaagcaaaga gttcttttac acaatatgaa aagcattgag acattggcaa
     1081 gggtaaatgt cctttgtgta gataaaacag gaacgatcac agaaaataaa atgtctgttc
     1141 aaaaaatgat cgtctctaaa aaacaagaaa taatcagtca gtcagatcaa ttggaagcat
     1201 gcattgctga ctatgcaaaa gcgatggcag gagacaatgc aacaatggaa gctgttaaag
     1261 catattttac aaaaaccact gatcaatcct atcaccatgt tattcctttt tcttctgtcc
     1321 aaaaattcag cagtgtagcg ttggcagaca aggtgtatgt tttaggagcg cccgaaatgg
     1381 ttttgcgcga tcaattgccg gaatatgcgg aagagtttgt ttctttcgct gaaaaaggct
     1441 atcgcgtgtt ggtctttggt gtgtacgatg ggattcttga agaaccagta ttgacagaag
     1501 cagtcacacc attaggctat attctgattg ctaatcctat cagaaaagaa gcaagagcca
     1561 catttgatta cttcaaaaaa cagcaagtag ccatcaaagt gatttctgga gataatccgc
     1621 tcaccgtatc caatgtagct gtccaagcag ggatttcaca tgctgatcag tacgttgatg
     1681 catctaaatt atcagaagaa gaatatgaag cagctgtcga aaaatataca gtttttggac
     1741 gcgtcaagcc agaacaaaag aaaatcttcg ttcaattgat aaagcgtaac aatacagtag
     1801 ctatgacagg tgatggcgtg aatgatattc tagccatgaa agaagcagac tgcagtatag
     1861 cgatggcttc cggaaatgaa gccgccatgc aggcctcaca agtggtctta cttgattctg
     1921 acttttcacg catgccagaa gttgttgcag aaggacggcg cgttgtgaac aatattgagc
     1981 gatcagccag cttgttttta gtgaagaata ttttctcctt tttgctctca ctgttttcag
     2041 tgatctttgc tctgacctat cctttagaac catcacaaat cacattgatt agtttgttta
     2101 ctatcggact tccatctttc ttgcttgctc tagaagaaaa caaaaagcga atcagaggga
     2161 aattcatcat gaatgtgatg gaaaaagctg tgccaggtgg actaacagat atgatcgtag
     2221 tcggtgcgtt ggtgatctgt ggggtgactt tgaatctaaa taaaacagat gtttctacag
     2281 catctaccat gcttttgatt gcagtagggt tcctcgttct ctataaaatc tgttcaccgc
     2341 tgaataaatt cagaagtcag atcattcttt tctgtgctag cggtaacttt ttttcggtca
     2401 tctttttgca taagctgttt tcaatcaccg gtatctcagc tgtatcactt ttgctgttga
     2461 gcattctatt tttctcggca gattcgattt ttaggcattt gaccacattg gtagaatatt
     2521 tgtttgaacg taaaaatgaa aaagagcaaa agccgtttag tcgcgtagct aaaaaatttc
     2581 tatcgatatt taaacgagaa aattgacgaa aagtatctca aaaaagagta aaaaagaaca
     2641 accactcgtg tatctttcga agcaggatga gtcatccaac tttaaaacgc acgagcagtt
     2701 gctctttttt acattttcgg aatggtaacg gtgaaagtcg aacctgttcc aagatgactg
     2761 tctacagtaa tcgtaccatt aaggagttgc acgtagtctt ttacaatcgc taaccctaat
     2821 ccggtcccgc ctgaatgtcg gcttcgcgcc ttgtctacgc ggtaaaaacg ctcaaagatc
     2881 cgttcctgat cctcaatgtc gatacctata ccaaagtctt ggacagaaaa cgatagatca
     2941 tctgttgcct gatagcggat aataatctcg ctgtcgcttt ttgaatattg gacagcattt
     3001 tcaattagat ttttgataat tggataaagg atatctgttt tagtagtaaa tggttcgttc
     3061 tttccaatca atcgaatcgt caaccgtttc ttttcgatga ttggctgata attttgaacg
     3121 atttgctgga aataaggttc tgtataaatt gtctggatct cgtaagaatt ctcgccatct
     3181 tttgacagct ggatgatttc ccgaatcagt ttatctaacc ggatcgcatc tttttgcatg
     3241 atctcaagaa aagaagtcag agtttgcggg tcatcttttg caccatccag taaagtctcg
     3301 gtaaaaccaa tcagtgaagt cacaggagtt tttaattcgt gagagacgtt gccaacgaaa
     3361 tccttttgta gcttttctaa acgtcgcact ttcgttaagt catacgccac ccctagaatc
     3421 tgtccggtac cttctgaatt agtaaagtag cgcaaactca agtctaatgt ctgccgacct
     3481 tctgtaaggg tgatctcctg atggatcagc ggatgctcag gagtcacttg atgaatcagc
     3541 tgaatgagtt ttggctcttg aatgacttct gtgtaccgct gttcaggttg atagcttacc
     3601 acgtttaaat gtagctgcat ctttggatta agcagtagta aacgagaatc agcatcatca
     3661 atcaggaata cgccaatcat cagttcgttc agtaacgtat ataattgctc ttctgtggta
     3721 gtacacgccc gatacatctt actcatttgt tcgctcaacg cattaatcgt ctgatacaat
     3781 tcttcccatt gatcgggtgt ttgcatgatt acttccgcct gattaggatt ttgcaccatt
     3841 ttttttagta cgggcagcac ggttttcaat ggctggtttt tttgatagat cagataatag
     3901 atcattcctg caagaacaag gaagaaagac agaaagaaga aaaagaccca acggcgaaaa
     3961 ctctcagttc tcggtaaaaa accgcttgtc ggttctgcga tacgtaaaat ggcttctaac
     4021 tggccacttt ttttgactgg taatgcgaca tataaaagat cctcattcaa agtagtactt
     4081 ttgcgcaaag cgctacctag gttcccacca ttcaatacgg ctttgatttc gggacgggag
     4141 tttcgctgtt cattcaatgt ttcattgttt gtgtcaaata agatctttcc agtagcatcc
     4201 attaaagtaa tccgttcgtt cgactgatgg acgaattctt ccaaagcagt tttgttttgc
     4261 gctgcttgga ggttttcgac atctaattgc cggatcagca gttgtgcttt ttttgtcagg
     4321 tagctttctt gctgttccac gatctgctgt tgaaaataat gggaaatcaa ttgccagcca
     4381 ccaaaaaaca aactaactaa aagaataaga aagaagagac gttcataaat ttttttccat
     4441 ttcatcgttt gggttcctga aacttatagc caaatcctct aatagtcacc aagtactttg
     4501 ggtgttttgg atctcgctcg attttttctc gcaaatgact aacgtgtaca tctacgattc
     4561 ggctttgtcc tgcgaagtca aaattccaaa tgcggtctag caatgtgtct cggtcgatca
     4621 cacggtcttt tcgtttcatg aaataaacaa gtaattcaaa ttctttcggt gttagttcaa
     4681 tcagatggtc gtctacggtc acttgataat tggtcaaatc agcagtgatt tgtccaatcg
     4741 atagatattc aggctcagct tcatcttttg attgcttacg aggttcaatt cgtctgaata
     4801 ttgctttcat acgggctaat acttcgcgag gactaaatgg tttggtcaaa tagtcgtcag
     4861 cacctatttc taggccaatg atccgatcca cttggtcatc tttagccgtc agcatcaaaa
     4921 tcggtgtttc atttttttct tgacgcagcc gctgagtgat ctccatgcca tccatcgttg
     4981 gaagcatcac atctaaaatg atgaaatcaa atgattgttc taatgctaat tctaatcctt
     5041 cgcctccatc tgtggcactg gttaccttgt agccttcttt ttccaaattg aacgtcaaga
     5101 gcgttacaat agaaggttca tcatcaacaa ctagtatttt tttcattcaa atctccttta
     5161 aataggtttc attctgttta tattttagca tagaatcagt acaaagggct attttacaaa
     5221 agaaataaat ggtataatag gaaggcactt tgctgaatag aaaggaagag cagtcaatga
     5281 ttggaatcag tgcatgttta ggcggtctgg cctgtcgcta tgatggacag aaaaaagaag
     5341 taccagaatt gatggaacta gttgaaactg gagaagctgt catggtctgt ccagaagtga
     5401 tcggcggatt accaattcct agagatcccg ctgaaatcat tggtggaaat ggattcgatg
     5461 tgtggaaaga tcaagcaaaa gtatggacga caagcggcga agatgtaacg gaagcgtaca
     5521 aaaatggcgc gatcaaagcc tatcaaaaat tgcaggaaaa acacattgat ctagtcatca
     5581 tgaaggaaaa aagcccttca tgcggaaccc attcgattta tgacggaagt ttttccggaa
     5641 taaaaaaaaa tggtgccgga gtggctactg cttattttat tcagcaaaaa atgactgtgc
     5701 tctctgatga agaatggctg aaaactagag gtgagcagaa tggaaatcga aaagaccaat
     5761 cgaatgaacg cgctctttga attttattcg accttattga cagaaaaaca aatgaactac
     5821 atggaattat attatgcaga tgatttttca ttaggtgaga tcgcggaaga atacgaagta
     5881 agccgacaag cagtatatga caatatcaag cggacaagca agatcttgga ggattatgaa
     5941 aaaaaactcc atcttttttc taattatatt gttcgagaac aggtattgga aaatatgact
     6001 gcatatatta cagataagta cccggaagat aaaaaactac ttgaatatgt acagcaaatt
     6061 caagcaatcg aagaataaat aaaaggaatg aagaaaatat ggcatttgaa agcttaactg
     6121 aacgtctcca gcaggcgatg ggaaaaataa gaaaaaaagg aaaagtatca gaagctgacg
     6181 taaaagaaat gatgcgggaa atccgccttg ccttactaga agccgatgtt aacttgcaag
     6241 tagtcaaaga ttttactaag cgcgtacgcg aacgtgcggt aggggcagaa gtgctggaaa
     6301 gtttatcacc tgcacaacaa atcgtaaaaa tcgttgatga agaattgacg atcacacttg
     6361 gttctgaaac agcagagtta aataaatcac caaaaatacc aactgttatc atgatggctg
     6421 gtcttcaagg ggcaggtaaa acgaccttta ctggtaaatt agccaattat ttaaagaaaa
     6481 atgaaaatgc tcgtccgctc ctaatcgcag gggacgtata tcgaccagct gcgatcgacc
     6541 aattgaaagt attaggtcaa cagctagacg ttcctgtatt tgatatggga actgaagtca
     6601 gcccggtaga gatcgtccgt caagggatgg agttagcaaa agaaaagaaa aacgactatg
     6661 tattgatcga tacggcagga cgtctgcata tcgatgaaac attgatggat gagttgaaac
     6721 aaatcaaaga attgacgcaa ccaaatgaga ttttgcttgt tgtcgatgcc atgactggtc
     6781 aagatgccgt caacgtagct gatagcttca accagcaatt aggtattaca ggggtcgtca
     6841 tcactaaact tgatggggat actcgaggcg gggcagcact atcgatccgc tcagtgacag
     6901 gcgcaccaat caaattcatc ggttctggtg aaaaattgac tgacttagaa gtattccacc
     6961 cagatcgtat ggctagccgt atccttggta tgggggatat gctgacattg attgaaaaag
     7021 cgcagcaaga ctacgacgag aaaaaagcgg aagaacttgc acagaaaatg cgggaaaatt
     7081 cattcgattt caatgacttt attgaacagc ttgatcaagt gatgggtatg ggcccactgg
     7141 aagatctaat caaaatgatc ccaggaatga atcaagtccc tggaatcgaa aacgtcaagg
     7201 tagatccaaa agatgttgaa cgcaaaaaag cgatggttta ttctatgacg ccagcagaaa
     7261 gagcaaatcc tgatctgttg aacccaagcc gtcgtcgccg aattgctgcc ggttctggaa
     7321 acagtgttgt tgaagtgaat cgaatgatca aacaattcaa ggaatcaaga aaaatgatgc
     7381 agcaaatgtc caaaggggat atgaacatcc caggtatgga ccaaatgttt ggttcaggcg
     7441 tcaaaggaaa actaggcaaa atggcgatga atcgaatgat caagaaaaat aagaagaaaa
     7501 agaaaaaacg gaaataagat gaaaaaaaca gcttcggtac agactgaaaa tgtctactgc
     7561 cggagctttt ctatcagcaa tttttattct ttactaatac aattactcta aacgaagagg
     7621 tggcacagat gagatgtccg tggagtgaac aaacagaaag tatgcggcaa tatcatgata
     7681 ctgtctgggg tgttcctgaa tatgatgacc aaaagctgtt ccgaaaattg atgcttgata
     7741 ttaatcaagc cggattgagc tggcagacga ttttaaataa aagtgaagca ttcgatgaag
     7801 catatgatcg attcgatatc aaaaaagtcg caaattatgg agaaacaaaa atcagtgaac
     7861 taatggaaaa caaaggaatt attcgtaacc gaagaaaaat cgaagcagcc gtccaaaacg
     7921 ccaaagcagt cttgaaaatt caagaagaaa tcggcagttt ttcagagtat ctttggtcgt
     7981 tttctaatgg aaaaatcgtt gattcttctt atcattcaca agaagaagtc ccaacaacaa
     8041 gtcctctttc agatgaatta gcaaaagatc taaagaaaag aggatttaaa tttatcggaa
     8101 gcacaacaat ctatgctttt ttagaagctg tcgggatcat caatgatcac cttgacagtt
     8161 gtttccgcaa agaagaggtc gtacgtatcg gcagaaaggg cgaataaagc atgcgaaaaa
     8221 tcatatttta tggtgctata agtttagatg gctatttggc aacgaaaacg gatgatcttc
     8281 aatggctttt tgacactcca acaggtaagc aaactactta tgaagctttt tatgaaacaa
     8341 tcgatacgac catcatgggc agaaaaactt atcaggaagc aaaaaaatat ttggagaccg
     8401 aagcaattta tccagagaaa aagaatttcg ttttttccac ccacgcacaa ttacaattgc
     8461 cagatgctga ggtcgtaaac gaagatcctg ttgcttttct aaaaaatcta caaatgacag
     8521 aaggaaaaga tatctgggtt gtgggtggcg gtcgattgat caaaccgcta ttggaacatc
     8581 aaatgattga tgaatggtat atccagatta cccctgtatt attaggggac gggattcctt
     8641 tattccaaaa aggaacatat gaagcgcgtt ttgaaatgct agacacgaca cgattcggtg
     8701 aatttatcga attgcattat gtacgaaagt agaagtaaat aaaataaaaa aggcaactat
     8761 atgcagaaat gtcctgtaca tagttgcctt ttttattacc gttatgcttt ttactctgtt
     8821 aatttgttag aaaaatctag atacgcatct aaaatcgttt ggaagaaact taatgttcct
     8881 tcaacgatat taccattttc atcaattaga ttcgcaacgt ttccgatata tgcttccggt
     8941 tgctgcaagg ttggcacatt caaaaagaca agtgattggc gtagatggtg gtttgcgcca
     9001 aagccgccga tcccacctgg tgatgcagta acgactaaac ctggtttatg atcccaaaca
     9061 ctgtgtccat aaggtcttga gcctacatct aaagcatttt tcaatactgc tggcacagaa
     9121 cggttgtatt caggagtaac gaaaacgaca ccgtctagtc ccctcacttc ctcacgaaaa
     9181 cgagtccatt cagcaggagc ctgttctgga gtttcgagat cctcattgta aaatggcaga
     9241 tcatcgattt ttacaaatac actttcgtac ccttcaggaa gcaatttcgc aaaagcttct
     9301 gctacttttt tgttgtaaga atcttttctt aaactaccaa taaaaaatcc aatttttttt
     9361 gtcatacgat ttttcctcct tcaaactatc tataataatc ttaca
//
LOCUS       DAZULY010000117         9317 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000117 DAZULY010000000
VERSION     DAZULY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.118"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..353)
                     /locus_tag="ACG81A_001782"
     CDS             complement(<1..353)
                     /locus_tag="ACG81A_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFS5015142.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDR"
     gene            complement(343..2517)
                     /locus_tag="ACG81A_001783"
     CDS             complement(343..2517)
                     /locus_tag="ACG81A_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HFS5015143.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2533..4875)
                     /locus_tag="ACG81A_001784"
     CDS             complement(2533..4875)
                     /locus_tag="ACG81A_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFS5015144.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4897..7440)
                     /locus_tag="ACG81A_001785"
     CDS             complement(4897..7440)
                     /locus_tag="ACG81A_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5015145.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7450..7848)
                     /locus_tag="ACG81A_001786"
     CDS             complement(7450..7848)
                     /locus_tag="ACG81A_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFS5015146.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7929..8213)
                     /locus_tag="ACG81A_001787"
     CDS             complement(7929..8213)
                     /locus_tag="ACG81A_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015147.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8237..9235)
                     /locus_tag="ACG81A_001788"
     CDS             complement(8237..9235)
                     /locus_tag="ACG81A_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5015148.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2650 a         1998 c         1459 g         3210 t
ORIGIN      
        1 tttctatcat gaatcacgag aaaacttggg acacttttaa catggtaacg ttgtaaaaaa
       61 gtttgaagtt cttcttgtct tggttctttt cttgtttcaa ttcgatatac attcctagag
      121 aattttttgt cgtatctttc caagaaagat tcaacttctt ggcatttttt acaatcattt
      181 cttgctagat acacaaaaaa tgattgattc gttttttgaa gttctagtac ttcttctaaa
      241 ttactattga ttagttttcc atttgattct tcgttagttt ttatcagttt aaaacaacca
      301 aataagagga aactaacaat aagtatccta acaataactt tcttatttcg catattgttt
      361 ctccaaattt ggtttccgaa tatagacgac tcgcggtggt tcctggacgt cttcagctgt
      421 tcgatacacc acaaattcgc ctgttctcaa tgctcgaatg cgattcggat tgaatttaaa
      481 gcggtctacg tctcgtttcg tgcctctttc tcctgtccaa tcaacctttc ccatatcaga
      541 ataacctgct tcttgtgtcg tcatttccgt taattcaatg tcttcttctg tccccattaa
      601 atctgcccaa taatcaattt ctgtcgggtc atttgtcttc ccaataaaga acgtattcac
      661 attcccaatc aactgttttc gaaggacagg atcaattcta tcaatgtccg caagcgtttg
      721 aggggcatac gccgcatgca ccccagcgcc tcgtccctta ttgactaagt ctataaggtt
      781 atcatttaga taagaagctg gttcgtcaaa tattactagg actttcgagc gattggctgt
      841 ttctcctgct tgtttataca cttctgacgc aaagaaatta atatctccca caagcatttg
      901 agccaatgtc cgaataaact cactataaat caatccgttt aacgagatat aaacaatctt
      961 tccttcttta acaacttctt gaatatctaa ttctcttttt cctccagaag tatcaaagag
     1021 ttcaccaagc tcactatcca ataacatatt gatattggtc tgtaaattct cagaagtcgc
     1081 atacaaataa aacgggttct tcttatgttc gtaacgaaca aacaaccgtt taaacaacgt
     1141 ttgctcgatt tctgtcatga gagaaatgtt ttcttttaat aatagataat acgtttctaa
     1201 gtcaacggtt ttacgatcat gttcaacgat tcgctcttca cttaactgtt ccacatcggc
     1261 aaaatcacgt tctgttttgt ccatcgcttc attctttttc gtttttttct tcgtggtttt
     1321 cttcttttct acgaccactt tttctttaaa taaatctgga tttttcaact gaatacgatc
     1381 cgcaaacaaa tgtaagacgt ttcgtggcaa taaataatgt tgtaaccaaa ctagatttct
     1441 ttcaatttta tggctctttt ggaactcgtc taaaagctga atcgttacct gtaataacgc
     1501 tttggctgct gtcgaataat acacgctttc tgtttctgct agattggtta atttatctcg
     1561 aatggcaata tcattgccat tttttacagg attataacgc aaatcgccac cgtccacaaa
     1621 gacttcaatc gggcgattgt atgcctctgc cacttctttt aacgcttgta aagtctctcc
     1681 tgctcctttc ccatcgacta agataacggg gatacgaaac ttacacgcat gttctgctag
     1741 taattggatc accgtcgttt ttcctgaacc tgtagtcgct ggaataatca catgttggtt
     1801 caattcacta aagttcatca cataagggag cccatactca tctgttccta atagaatcac
     1861 tttggctaat gaagccagtt gtttattccg caaagcaacg tcttttatga caattgctac
     1921 ctggtacgct tgctgatact ctgctgtata tttttgggac tcctgaaatt tattcttccg
     1981 acgttccttg tattctttac tatttagcaa agctgtttgc cgttctttct ttgaaacaac
     2041 ttttcttttt tcaataaaag acgcatacgc aatcaaacaa cttcctgcac ctgtcccacc
     2101 taaaaggagg gcaaaaatcg tttgtccatt aaactgcaac gcatgagaaa acaagtttaa
     2161 tggaaataat aagtaacacg acaaataccc tctaggaaga acaaaaataa aacaaaatag
     2221 atttaatagc gtaaaaccta acgcaaaaat aaaaaaaaga gagcttcttc gttttaagaa
     2281 gctctcatac tcaaagtcat aaatatgtgc acgtgtctct tttttatcta tcataaagac
     2341 aatcgctaaa ggaatactgc ctacaataaa aaatggatat agacaaacac ctaataaagt
     2401 gacgataaaa taaagttgtt gaaaccgact ttttggcgtt tctttttctt gttttctttt
     2461 gcgatcgaca gaaaacattt gctcaaaatg tgtcggtcgt tttggtgcat tcatcatact
     2521 atcgatttcc tcctatcgtt ttcgttgttt catttctctt cgataaatcg gttgtttttc
     2581 tcgttttcgt ttcatttttg cttgagttgc tcgaaactca ctccgtgtct tcggtgcttg
     2641 ttttggtgat tttgtttgtt tcatggttga gtatgcttta gatacggttg aaggcgcttt
     2701 ggtttgtgct gttactcgat tgactgctga catatgttcc cgaaccccct ctggacgtcg
     2761 aacagatttc ggtgtggtag aacctgttgc tcgtggtaca ttcccttttc ttagtggttt
     2821 ttgtaccgca tgtttttgtc ctgcaactgt ccgggttttc atcgaacttg tttttgttac
     2881 gggagtaagt gcctccttcc gacgacgact tgtttgtccc tgacgacgtt ttcgatcgtt
     2941 caattctgtt ctaggcgtat gcttctggcc ataagacttg tgtttttgct ttggattcgc
     3001 taatgacacc accattgcag aaccctgatt gcgatacgga ctacgagacg caccttttct
     3061 acttctttct cttagactag gattgccttg caattctcgt tgtaataaat cggctgtttt
     3121 tcttgcctgt ttctccattg ctgttcgttg catacgttct tgactttgct cttctgcttg
     3181 tttcgcttga tcatgcaagt ctcttgcttt ttgtgccata cgttccatac gatttggtgc
     3241 tttatgacga ggttgttgtg cggattcacg ccgaaaagca cttcgtgtgg cttgtttcgc
     3301 cgtttgtggc atacgaacgt ctgagccacc cccacgtttt cgttgacgtt cttgtctctt
     3361 ccgtgcgttt tctttttctt tttgtttctg tgcttttctc gcttctttgc gttctttcaa
     3421 ccgttgtttg gcttcccttc tcaattttgc ttctgcccca aatggatttc ccgcaaaagc
     3481 taacgctcct cgaacaccca ttcttccagt aaatagactg cctaaaaagc ctcggaacaa
     3541 ataaattccc aacggcgcaa acaaataaac aatgatcaag agaatttttt cccatggatc
     3601 gtcttgtgta ctacttaatt ggaacccgta actaagaaaa gaagaaaaaa ggatacatgc
     3661 aaaacctgtc attcctttta aaaacgtcaa aataccaaaa actttggcat agttaccaaa
     3721 cacattcatt gtgacaagaa acaatcctaa aaaaagtaag aacggcaaca tcggtactaa
     3781 caatagttgc atagttccaa taatcaatcg aaacatactt aagacaaaat aaacaacggt
     3841 ttgaatcaga ttcacgacaa aataaaacaa ggcaaatgct gtatttttcc aactattgta
     3901 atattgtatg ttcttgtttt tcagtgtatc tgcttcatac tctgtgactt tgtcatgaat
     3961 tttctcccct atttttgtac ctaaatcatt gtccaataaa agattgattc gatcatattc
     4021 ttttccagca tatttgactt tcttctcacg aattgtgtct acattggttg tgccataatt
     4081 taacaacaga tatggttcat aaacattact ataaaagacc cgagccgcca tcaatttgcc
     4141 tgccgtttcc acttttttat ccgctgttac ctcttctgtt tcttttgttt cataatttgt
     4201 ttcttcttca ggtgttaaca ctggattgac cttcacgaac gccgtttcca ctgtagaatc
     4261 taaagaaaaa agaacatcaa aaagattctt atttgtccca gaatctttta atacgcttaa
     4321 cccagtaaaa ataaggatcg tcataagaaa gacacggagt gcttgtttca ttcgagcctc
     4381 tccagcatat ttgattgcca tcataattgc cgaaaaactg attccaatag ttcctgcaat
     4441 acttaacata ttgtccgcaa ttttttgtgt taagttttga atgggttctc gaatggcgga
     4501 aataatatct aattcaaaga gaaattcaac cattgaagca ttgaattctc ctaacatttt
     4561 tacgccactc caaacaaaat ttttcatcac tcctgcaaca ttgtacgata gctttttttt
     4621 aaggttcatt gactcatagt cattggtcat caattcaaac gaattctttt tgtaattttt
     4681 atacaattga tccacatcag tcgttacttc ttttacttct actttatcgt tggcattctc
     4741 tccttcagaa cgatcggttt cagtgacacc cgcaccatca ttcatttgat cggcagaaac
     4801 agtagcccct tgtagacaga aaaaaagacc ccctaataaa aggagcgtcc accatttcag
     4861 agattttttt cgcaaaaaaa ccacctcttt tttgttttat cgttgttttt cattttctaa
     4921 cattgcttga attttttcat cttgattttc agtagaagaa acagccaata accaactatc
     4981 aaacaacaca tcaatggcaa tcggttgatt tcttcccaaa tgatcctgga ataaacaggt
     5041 tcctgtttgt aattgttcca tcatttctaa attggctgaa ttcacttcca tatcaaagaa
     5101 ggacaatagt ttctctcgtg cttctttttg tttcggacga aacgcaaatt tgtaagaaat
     5161 taactctttt ttatcttctt gatcgtaatc tttaaacgct tgcacaacaa ggtaaatatc
     5221 cgtattattc gcacgtcctt ttcgcaagct atcatcaatc aaatagcttc cttgtgcggt
     5281 atcttcatac ccttttgcct cgtcataaat aatcgcagcg tcttcttctt tgtttgtaga
     5341 aaagatatac gtaaacttca taatgacttc cataattgcc attcctgcaa gttgttcgct
     5401 atttaatcga cccgaatcaa tctctttttg tgtcggaatg cgtattcctt gtgtccctaa
     5461 cacgttgatt tgtgaatcaa aacgtatcgg cgtactgtgc tcattcccaa tgagaatctt
     5521 tcctaaacca acgctatgcc ctaaaatcaa tttagctagt tctttgtcac tttttcgaat
     5581 ttcttcgata acttgactta acgaatgctt acctggcatg gtgtaaacta catcgatggc
     5641 gtcttggatc accgttttct tgtggctcgc tgtttctcga ttggtatcta cttctcctaa
     5701 cattcctaac acatttttag cggtttgaat tgcctcttct tttggtaaaa acaatagagg
     5761 atcaagcatc cctcgacagc tttcatctga agataacgaa ataaaattaa ttcgttcata
     5821 catagccaca aactctggta tgtgtccgaa tttttcaagt gcctttaaaa agaacttttg
     5881 ggtttcattt ttcggatcaa tataaagaat tttttgacct aaaaacatca gccataaaaa
     5941 aatatatttt acaaggaccg attttccttt acctggtgga ccaataatca acgtatttcc
     6001 attggtatgt tccgcgcctt caatactttt ctttgttaaa ttgggataat accatactag
     6061 tttactagat agcgctaacg cttctttcgc agatttaaac ttttttgcaa taacaatccg
     6121 tcctaaagga aatcctttct tatttcctac ttctttcttt aagtccaagc cataatctgc
     6181 cacaaaacct gtcgtagcga cctgctcgta actacgaaaa cgttgcgtac ttccaattaa
     6241 gcattgatta aacaacgtta gctgatccgc taccgatcga taaaatttga aatctgtaga
     6301 ctcaatcgca aattcaatat ctttgactcg ttcttccaat tcttcttttg aagaagcaga
     6361 tacaaccagc gtgacggtag aataacacaa tcgtttttct ttgcgcttca gcttatcttt
     6421 tacatccatt aaccgttctg taccaaaatt taatacatcg tctgaatcta aaatgccgtc
     6481 aacggtctca tattcttttt cttgttcata aatccgtcca gccattttat taatttttcg
     6541 ttcatcagat tttgaatgct caaatcgaag tcgaatatgc gtatcaattg gaaaagataa
     6601 gctatcctga agattttgaa taaagctact cccatacatg gctaaaggag cgtccgtttg
     6661 acttaaaaac gtcgaataat gcgtttgatt caactgttca atcgcaatgt atcctgcttc
     6721 attcgaaatg acgccctcgc aaatttccat tggcgtaagc tgtctttcag gcaatttttc
     6781 ttctgatcga tggaaaaaat aataataaat ttgtccaatc tcttgggcag ataagcgctc
     6841 tatcggctta taattggaca attccgtata gagctccttt tctaatgttt catacgttgg
     6901 taacacacta cttataggaa ctcgttgtcc cgtaatacga tagataaact ttttaaaaac
     6961 atgcttaagc aattgaaaat attcgatcgg atcagacact ttcatttctg tagtgaaacg
     7021 cacaaacaga tacgttcgat actcgtttaa ttgcacttcg tcttttaaaa tctttcctgc
     7081 ctgttcaaag tacactttcc caagatctgc gcattctccc tttaccaaat ctcgaatggt
     7141 ttcttcaatg tgcgtatcta ggttaaattg acgtggaata ttgataaaat gatagtcata
     7201 ttcatcatgt ccaagtatgc cttcgccatc ttgaagatag ttcttgaaaa aatcaatgtc
     7261 atttaaagga aatacttggg aagaaagttg atagcctacc cacacatcat catttttggt
     7321 caaaacaaaa tttcggataa tggcgtctat cggaaattct aatcgaggaa gcttcgtttt
     7381 tttctttttg aactgccaaa tcccgataat aaagacgacc aaaagaaaca aaaacgccat
     7441 ttactcgact cactccttcc atttgtgtag ggaatacgtc actttttcat ggaaaaacgg
     7501 cacatacatt tcatgttcaa agcgttgttt ccgtttctga taataggacg ttcgcccaat
     7561 taaatactcg aaaaagtgtt tattgtccca tttcattgaa aacaatgtcc aaacgagaac
     7621 accaatccca aaaatcataa tcaaccagga ctttcgaaac agtgtctgca agaaactaat
     7681 ttgattcaca tacgcaatca aaaataggat caaaagaatg aagagaaata ccccacctat
     7741 gactaattgt tccatgcgaa tgccattaga aatccaggga attttttgtc ctttaatccc
     7801 gtaaattttg tgagggtgct taaactcctt tgtatagttg tattccatgt tcactctacc
     7861 tccttttaca aaaaaagaag cgaccagaag ttctaatctt ctgatcgctt ttgcagtttg
     7921 tctttttctt atgataattg tatccagggc acaatcattt gcgttacccc cacgttctct
     7981 tcactatcct taataaagat aatgttcttt agccattcac caatatcaac ggcattcgat
     8041 aacaataaga cgagcattat caaaataaag acgccaataa tggcaaacgc tgcccgcgca
     8101 aagccttggg tcgcataagc accaaccatt agaacaatcg caacgattgc caatagccaa
     8161 atgacttgtc cagatacatc atcaaataac gatttaagat caaaaagttt catttccatt
     8221 ctcctctaag ttcattttat tcatttaacg tagtaacaaa ataactatcg ccacttgttt
     8281 ttacttccat tgaaaagttt gattggaact cgtttccttt ctcaaaatag aaacgatact
     8341 tcccttgcaa aaagtagttt ccttctttat tttttactgc atgatccagt tgtacattga
     8401 tcaattcgcc gccttctaac cctttttcgt tagaaatcaa tgccaatttt tcatcatttt
     8461 tgacatataa ctcaaaaaac cgtttcacaa atgcttccag cttgatttct tcgctttcag
     8521 tgaccgcttg cccagaagaa taaaatcgat tttcctgata gttcacagaa ttggtattct
     8581 tactctcaga aagattcaaa aattgaacgc catcaagtac ttttaaaaca ttgttttgat
     8641 aactgactgg aaaagaaagt tgactgcttt tccaatgttc tccttctgta aaagtaacct
     8701 cataaaatag gcgatacatt gcttgtgtca aatcgatttc ttctagttta ataaagcgaa
     8761 catctttcgc tactcgattg gtagccgtcg ttacaaagcc tagatcagac gcttccaatc
     8821 cttgtgctaa ataatgattg acggaagcct cccattcgtt tcgtttttct ggcgaataag
     8881 taaataacct ttcaataaaa tgttgtccct cgtacttcaa ttgttccgta gaagacaagg
     8941 tttggttgac ttcagttaac aatgtttctg gattgacttg ttttttcact gctaaacgcg
     9001 ttaatgtatc cactcgtcca aagtttagga ggagcacgac acccattaac agaaacaaac
     9061 aaaaaaaagc gcctgaaaat acactcatgg cacttttttg cgaaaattgg atcattcgat
     9121 ctgacggttc tgttttcttt ttttggaaca aaaaacgttt cttttgtttc ttttccttct
     9181 gttgtttcgg tggcttagtt tggaacgctt gtggattaaa ttttgcatta ctcatgagaa
     9241 atgccatacc gttctaacaa taatttcggt attttgtcca caatcactac ttcgtggaca
     9301 accacacttg ccccctc
//
LOCUS       DAZULY010000118         9194 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000118 DAZULY010000000
VERSION     DAZULY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.119"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            21..668
                     /locus_tag="ACG81A_001789"
     CDS             21..668
                     /locus_tag="ACG81A_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFS5015149.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            752..1696
                     /locus_tag="ACG81A_001790"
     CDS             752..1696
                     /locus_tag="ACG81A_001790"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFS5015150.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1831..3282
                     /locus_tag="ACG81A_001791"
     CDS             1831..3282
                     /locus_tag="ACG81A_001791"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFS5015151.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3303..4139
                     /locus_tag="ACG81A_001792"
     CDS             3303..4139
                     /locus_tag="ACG81A_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS5015152.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4139..4798
                     /locus_tag="ACG81A_001793"
     CDS             4139..4798
                     /locus_tag="ACG81A_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5015153.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            4924..5613
                     /locus_tag="ACG81A_001794"
     CDS             4924..5613
                     /locus_tag="ACG81A_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015154.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5619..6575
                     /locus_tag="ACG81A_001795"
     CDS             5619..6575
                     /locus_tag="ACG81A_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFS5015155.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6619..7131
                     /locus_tag="ACG81A_001796"
     CDS             6619..7131
                     /locus_tag="ACG81A_001796"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5015156.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7387..7935)
                     /locus_tag="ACG81A_001797"
     CDS             complement(7387..7935)
                     /locus_tag="ACG81A_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS5015157.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(8024..>9194)
                     /locus_tag="ACG81A_001798"
     CDS             complement(8024..>9194)
                     /locus_tag="ACG81A_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFS5015158.1"
                     /translation="LGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGFVLLR
                     TTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISAIIGI
                     LRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFLSEKR
                     LFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEEKEDE
                     LIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGITFQEI
                     LGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTAIISV
                     FLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLLV"
BASE COUNT         3043 a         1688 c         1866 g         2597 t
ORIGIN      
        1 taacaaaggg gatcgaatca atgaaaaaaa ctgttattta tactagttta tttgcagctt
       61 tgtttttagc tgcttgtcaa tcaaatacca atcaaggcac atctgggaat acgtcttcca
      121 gtagcgaaca gcgctcttca caggtatcgg actcctcaac aaaagcaagc acatcttcgt
      181 ctagccagac aaaaagttct agtgaagcaa gtactcagtc agatatcaaa gtgtctgcag
      241 ctcgtgcgat cgaattgttc caagataaat acccagatac tgcaattact agtttggaat
      301 tagatagtga ttggggatct tacttctaca agatagaagg tgtcgatgat caaaaagaat
      361 acgaagcaag aattgatgct caaaatgaaa aaatggagac agagaatcca gaaactctag
      421 atcgagatga acaaaatggt caaaagaaaa aagaagatgg tctggatgtt gataaattga
      481 tcacgattga ggaagcagca aaaatcgccg tcgacaaagt aggaagcggt acggcaacag
      541 attgggattt ggataaagat ttaggtacta cttattggga agtcaaagta aaagacggca
      601 atcaaagtac atcagtcaag atcaatagcc aaacaggcga agtactgtca actgaacaag
      661 atgattaaga atttcttcgg aaaacaagag ttgatttttc ttttagtcat gatacactag
      721 acaaaataac ttgtttgaag gagcgattta cttgagttta gaaacgacct ttgccaatca
      781 tatctttgcc aatcctttga tgaatgcatc tggtgtccac tgcatgacta cccaggaatt
      841 agatgaactg gctcattcag aagcaggagc atttatcact aagagctgta cgatcaatga
      901 acgaaaagga aatcccgaac cacgctattt tgatgttcct ttagggagta tcaattctat
      961 gggacttcca aacttaggtt tttcttatta tctagagtat gctcttgcgt atgaaaaagt
     1021 gcaggaaaac caaaatcaac cgttattttt ttccattgcc gggatgagtg tccaggaaaa
     1081 cttggaaatg cttgagaaaa tagaaaaaag tgggtttaat gggatcactg aattaaattt
     1141 atcttgtccc aatgtgcctg gaaaaccaca actagcttac gattttgagg ccacttatga
     1201 aacactgaaa gaagtattca gcatcttttc taagccgcta ggaatcaaat tgcctcctta
     1261 ttttgatttt gctcattttg atcagatggc tgatatcttg aatcagtttc cattgaccta
     1321 tgtcaatgct atcaacagtg tgggcaatgg tttgtatatc gatacagaac aagaagcagt
     1381 agtcatcaaa ccaaaagaag ggttcggcgg cattggcgga gagtatatca agccgactgc
     1441 tttggcgaat gttcgcgctt tttatacacg tttgaaacca gaaatccaaa tcattggtac
     1501 aggtgggatt cggacaggac aagatgcatt tgaacatctg ctatgtggcg caagcatgtt
     1561 acagattggt accgagcttc ataaagaagg gccggaaatt ttttctagaa tcataaaaga
     1621 attaacgcag attatgtcag aaaaaggata tacgtcaatt gacgaattta aaggcaaatt
     1681 acgcaccatt tcatagcaaa gagacgaagg tcatgattga tcttcgtctt tttttataaa
     1741 aaacactcgt ctgttcattc ctgtgcaagc gtactcattg tatgttagac gaacttgtgg
     1801 gaaaataaca gaggcaaaaa ggaggataat atgaatgatg caacagaatt agcaaaagag
     1861 cttaaagaaa aagaatttag ttttcaagaa ctagttacgg atatacagaa aaaaataaag
     1921 aagcaaaatc atgaattgaa tgcgtttgtc gcgcttgaac cattcgagag taattcccac
     1981 gagctattga ttccccagtc agaaaaaggg ccattggctg gtatcccatt tcctttaaaa
     2041 atgctcggtc aagaaaaaaa gggatggcta gctacttcag gatctcgttt atttgaaaca
     2101 caccgagctt ctcatacatc taattatgtg cggcaagcag aagcgatagg tctagttcct
     2161 tttggacaaa ccaatgcgcc agagtttggt ttcaaaaata ttacagaccc agtcatctat
     2221 ggcccagcaa gaaacccttg gaaccttgaa tgtacaccgg gcggatcaag tggaggagct
     2281 gctgctgcag tttcatcagg aatcgttcct ttagctggtg ctagtgatgg cggtggttct
     2341 attagaatac ccgcttcgtt ttgcggattg attgggctga aaccttcgcg agggacgatg
     2401 ccagttggtc catatgcttg gagaggatgg caaggagctg ctattgattt cggcttgaca
     2461 gtttctatga gagacacgga ggctttattt tatgggatga gaagtatcca ttccggtgcg
     2521 ccatatcaag tatcgccagt tgagtggcaa acacatccaa gaaaaaaacg gttgaagata
     2581 gctttttgtg ttgattcacc gatccactca cagattagtg aagaggcagt acttgctgta
     2641 aaaaaagctg tagatttcct tggaaaagaa gggcatgatt tgacagagat tccttaccca
     2701 ctagatggtc gtaaactgat cacgtcttat tatcagatga atggcgcaga aacagcagca
     2761 atgatccgct cgattgaaac aggcatccag cgagctgtca gaaaagaaga acttgaaccc
     2821 atttcttgga ctttgttcca atatggtcag aagctctctg ccgctactta catcgattct
     2881 ctgcatgtat gggatgaagc agcaattgtc atggaaaacc tacatcaaga atacgatcta
     2941 ttcctttcac caacgactgc atttaccgcc cctaaaatca gcgaagattt gcaaagtgat
     3001 gcgataagag cacagatgga acacatcagt gattgttctg aagtagaagg tttagacgtt
     3061 gtttcttcca tgtttgagga gagcttgaag atcacacctt atacacagtt ggctaatctg
     3121 actggacaac cagcaatcag cttgcctact catttgacaa aagaaaacat gcctctagga
     3181 atccaattta tggctgccag aggaagagaa gatttgctat ttcaaatcgg ctatctattt
     3241 gaagcaaatc atcagttcca tttaccaaat ccatggaaat agcagaaaca aggagaagaa
     3301 gaatgaagaa aaaaagattg tttgctctaa taactagtgc cttttttatt ttaagcgctt
     3361 gcggtacagc tgccgctaat caggaagata acagctggag taatgtagaa aaaaagaaag
     3421 aattagtagt agcgacttct ggcacactgt ttcccgcatc ttattataat gatcaaaatg
     3481 aattagttgg ttatgatgta gatttggcaa aagaggtggc taaacggtta aatgtcaaaa
     3541 tctcctttaa agaatacaac gtggatggac aaatcacttc tgtttcaaaa agagaatcag
     3601 attttgctgc gaacgatttt ggtatcacaa aagaaagaga aaaaaaattc tccctttcag
     3661 agactttcaa atattcattt gacagtatga tcgtacgaaa aaaagatgat tcaggaatcc
     3721 actcacttga agatcttaaa gggaaaaaag cagctggcga acccaatact agctatatgc
     3781 gtttagcgga aaaacacgga gctaaattag tcacatatga caatgccacg aatgatcagt
     3841 atttaagcga tgtagcaaat ggtcgaacag atgttattct aaatgattat tatctgcaga
     3901 aaatggccgt atctgcttta ccagatatcc ctgtaaaaat actggagaac gtttatttca
     3961 atgaaaacga aagtggtttt ttattgaaag aaggaaatga tgctctgaaa aagaaaatcg
     4021 atggcgtatt aaaagagatg aaagaagatg ggacgacaaa gaaaatatct gaaaaatatt
     4081 ttcaaactga tgtttcagtc aaaccgaaag aaacgatggc tgagtcggtg agtgagtgat
     4141 gtactttccc ctaattgatt tttccctcat gtggacttca cttcctttcg tactgcaagg
     4201 cttggtatac acattaggga ttggcttcgt gtcgtttctt ttaggaaatt tggtagggtt
     4261 gcttttgaca gttttaggtc ttttggattg gctgcctctg aatgtattta ttcgttttta
     4321 tttatcgttc tttagaggca ttcccgcact agtcctgctg tttctgttat atttcgggtt
     4381 accttatcaa ttaagtgcct taacggcttc agtcatctgc tttacgatta ccagcagtgc
     4441 atttattggt gaaatctatc gaggatcact agcaggcgtt tcttcaggtc agtgggacgc
     4501 tggtttagct tctggttttt ctttctttgg ggtattgaaa tatatcgttt tgccgcaagc
     4561 ttttcgaatc tcagtaccgg cactcagtaa cgtagctatg gatctagtga aaggtacttc
     4621 gttagcagca acaatcacag tgccagaaat atttcaaaaa gcaaagatca taggcggtcg
     4681 gacattcgat tatttttctc tctatatttt agttgcactg atttattggg tcatttgtat
     4741 cgggatcgaa aaagtgcaac agtatttaga aaaacgttct atccatcagt caatctgaaa
     4801 aatagaaatg gtgctagcgt ggctggcacc atttctattt tttgtttctt gtatattctg
     4861 cctagtattt taaaaccatt tcatgtaaaa tgtaaatcga aaatatgttc ggaggtagaa
     4921 aacatgaaga ccccgctaac catcgaaata gaatcctcgt tataccaata ttgcatcgag
     4981 caaggcggct tggttgtaga agaagtcacg atgcctgatg accaaggaat tgtagatacg
     5041 cttagctgct tgacaaaatt tgatggaagc cgagaatggc gatgctatga gctgaaagtg
     5101 acaaaagcag attttcgctc tacagccaaa ctgtcatttg ttggacatta taattatttt
     5161 gttttaccaa gtaaactata tgaagaagta aaaactgaaa tccctacagg aataggtgtt
     5221 ttagtgtatc gtccgtacac ggttgaagag gagatgcctg cctctggtac atttctgatt
     5281 gcgaaaaaag cagcaaagaa agagcttgct gtaccggaag aagctttgac aaaccgcttc
     5341 atggcttctt tattcaggga agtaagaaaa gcaaagcaaa tggagcgagg cgtaaaatat
     5401 tttccaaaag atcaattgta caaagaattg aaaagacgtt cagaaaatca agatccattt
     5461 tctaacgaac gatattatga acggttttta gaggaaatag ctagtcaggc aatcaatgag
     5521 ttgcaagaag aagttatcgc gcttcagcat gattatgaat atttgaggca acaacagcag
     5581 atccgtcggt tgcctacaga gccattggag tgaaaaatat gtactatcct tatttaagag
     5641 gaaaacaatt tgacctatta gcattaaaag aagcactgtc gcgaggattg ctctccaata
     5701 aaatccaacc agtgatcgaa ccagtacgtg attccgctac gcttaaaaat gtgatcgaat
     5761 tatttcaaaa aaagcaccat ccaatcgctg tgatccaaaa tccccaagta ggacaattca
     5821 aattattcga tcagcatgtg cacagttgga cagtcaaaga aaatagctct gtcgtccctg
     5881 cacagatctt aacgccagaa aatctcagtg aagtactgga ttcacctcca gcgtttcttg
     5941 ttttcgatgg acagcactac ccaaaagata cagaggtgtg gaaacagttg gcaggagttg
     6001 attcgaaatt tttgatacca gatactagtc gctttcgcat ctggctgccc gaaaacaaaa
     6061 ttgtgatccg tgactctttt caaacgagaa agcatgttga aagttatgca gataaaacag
     6121 atgacttttt tagtgatgac tatctttttt tccacgcaga cggatatatt ggattttctg
     6181 atttcacgat cgaaggtagc cgttatttcg ataaagggtt ccctagccga gcgcttgcta
     6241 tccatctaac gtatattgat gcttacggca atatacgcgt gaaacatttt gtctctgaca
     6301 gtaacgactc tgctaaagac caagcattga agtttcttga agcaggagat aaaatgaaaa
     6361 aatggatcat gcgtcaccat catcaactct tgattacttc aggaatgatt gaattattgg
     6421 ctctttacca gcaacaaaaa ttccctggac tgggagtgtt gaaaaagtgg tcacttatgc
     6481 atcatttgga acttattagt cacctgctag atcatccaaa agattggctt aaatattatt
     6541 caaaagttca ggaactatat gaagtgatcc aatgattaga taatgtaaat gaaaaaggaa
     6601 tagaaaagag gaaactggat gtatattcgg caaacaagga aagaagaagt agaagaagtg
     6661 gtagaaatca ttaatgccgc agcggatttc attgcttcgc aaggctctcc tcaatggcaa
     6721 aacgggcaga aaccaacaaa agaaacgatt ttgaacgata ttgaaagaaa agaaagctat
     6781 gtattaatag tggaagatca aattgctgga acagctgcat tagtctcagg cgtggatccc
     6841 gtttacacaa aaatagatgg tagttggaaa gaatcaactg gtccttattt atcgattcat
     6901 cgtgtcgctt tagcagaaag tactcgagga aaaaaactag caaaaccgtt cttagaacat
     6961 ttgttgacga ttgcagccag caaaaatatc caagatatcc gtatcgatac ttatccagct
     7021 aatgaaccga tggaaaagac catctattct gctggattca cttatcaagg gatgattgaa
     7081 tttccttttg aacacggcga aagaaaagca tatcaaataa tcatcaattg actataaaat
     7141 catctgagca aagataaaga cattaacact actgaaaaaa acaatcatat gatagccaag
     7201 aggttgtaac agaagtggcc agcttcaaaa aacaagaaag aatttccgaa aatgcttctc
     7261 aaatttttgt ggaattttag cgtattttac gaaatagctg ctttctgaaa ctcccttcat
     7321 cgagaggtcg tgacatagct tttgtcacga tctctcggct tatcctacga gtattattcg
     7381 atacatttat atcaataatc ctttttttat tttatctgca gcttcttgga acgctccatc
     7441 gtttgacacg actaaaagtg aggggatagc tttcaaacgt ttcaactgtg ctaaatcttc
     7501 tacataaacc gccagtctgg ctgaaatctg ttcagatgaa tctccacgtt ttttcaggcg
     7561 cgattgcaat gtttctttag agatcgtaat aaagatagga cggactaccg tcccgaaata
     7621 ctcatataaa tgccaaaaac catttgctgt caaaacggcg taacaatttc ctactgtcag
     7681 tttcttttta atttctgctt tactggaacc atatttatgt tgagcatatt ggtcccactc
     7741 tgcaaactca tttcccttga tcatctgatt gaatgtattg tcagaaacaa aataataatc
     7801 ctcttgatct ttttccccag gtcttggtga tcgattcgtg taagtaatga tcttttgttc
     7861 cggctgaaaa atagcttctg cgagtgaagt ttttccagaa ccagaaggac cgataaatac
     7921 atagcaataa ttcattgtca tttctccttt gatgattgaa caagtgaaaa agctttggaa
     7981 aatatttcca aagcttttaa aagtaacttt gcttttttgt attttatacc agcaagccag
     8041 tcacaatagc acttaaaaag ctgactaaag tcgctgttag caaaattttc aatccgtgtt
     8101 ttgcgacaag atttcctttt tcttcattta atcccttcat cgcaccagaa atgatcccga
     8161 tagaagagaa gttggcaaat gaaacgagga agacagatat aattgctgtt gtacgctcag
     8221 taaaatggaa attaccattt gctaattctg tcatagcaac aaattcattt gtaaccagtt
     8281 tagtcgccat gatactaccg gcatccactg cttctttcca tgggattccg ctgatgaagg
     8341 caagaggggc aaagatgtaa cctaggatct cttggaaagt gatgccgaat atcccgtgga
     8401 agatagcatt gatcatggca atcaaagcaa caaaaccgat aagcatagca gcaaccgtga
     8461 tcgccacatg gaaaccatca agaatgtatt ctcccaacat ttggaaaaat gtctgttctt
     8521 tgttttcttc aatgatcagc tcatcttctt tttcttctaa tacataggga tttacaattg
     8581 aagccagaat ataaccgcca aataagttca aaacaagggc agtgatcaca tatttagaat
     8641 cgatcagtgc catataagat ccgacgatcg acatagaaac tgtagacata gccgatacac
     8701 tcatggtgaa caaacgtttc tcagatagga agggtaattc cttttttatg gaaataaaaa
     8761 cttccgattg tcctaaaata gcagaagcta ctccattata agattccaat ttgcccatcc
     8821 cgttgatttt acttaatcct agaccaacag ctttcataaa taatggaaga atcttgatat
     8881 agcgtagaat accgataata gcagaaataa atacaattgg catcaatacg gaaagaaaga
     8941 aaggtgcact tcctttattt gctacaccgg caaaaacaaa gtcaacacct tgcccagcaa
     9001 aagcaagaag atgatcaaac atttttgcca atacagaaac aaccgctgta ccaaaagttg
     9061 tacgtaataa tacgaaacca agaacaagct gtaaaacgaa catcacaatt agtggtttca
     9121 atttgattgc ttttttgtta ctgctgatca aaaaagcaag ccctaatgtg acaaataaac
     9181 caataatacc taat
//
LOCUS       DAZULY010000119         9022 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000119 DAZULY010000000
VERSION     DAZULY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.120"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..648
                     /locus_tag="ACG81A_001799"
     CDS             25..648
                     /locus_tag="ACG81A_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015159.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            1397..3490
                     /locus_tag="ACG81A_001800"
     CDS             1397..3490
                     /locus_tag="ACG81A_001800"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5015160.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            3650..4516
                     /locus_tag="ACG81A_001801"
     CDS             3650..4516
                     /locus_tag="ACG81A_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS5015161.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(4538..4975)
                     /gene="ndk"
                     /locus_tag="ACG81A_001802"
     CDS             complement(4538..4975)
                     /gene="ndk"
                     /locus_tag="ACG81A_001802"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFS5015162.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     regulatory      5148..5322
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            5664..8312
                     /gene="mgtA"
                     /locus_tag="ACG81A_001803"
     CDS             5664..8312
                     /gene="mgtA"
                     /locus_tag="ACG81A_001803"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFS5015163.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     gene            8399..>9022
                     /locus_tag="ACG81A_001804"
     CDS             8399..>9022
                     /locus_tag="ACG81A_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5015164.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGK"
BASE COUNT         2977 a         1544 c         1899 g         2602 t
ORIGIN      
        1 aattaaatag gagtggagga aagcatgagg aaaaaaagac cgtttcttct cttttggtta
       61 ttcggttttt tcttactgct tattttatcc gcagcatttt caggaaaaat gacagcagat
      121 cttgctcaga aagaaacaga aaaaatcgta gcaagcaaag aatcagtaga taatttcaaa
      181 aaagaattct caacttattg gtgggcggag tataaaataa ctgccaatta cgaaaaaggc
      241 gaattggaca tttatttgcc aaaaggaaaa gaagaaaatc aatttattaa tatcgatgac
      301 gtacttagtg cgatttctct cagacaatat caatttaaag acaagcattt gaccgttttg
      361 cttttgactc atgatggcga caaagtagcc cgtttgaaaa atggaaaagt gatcaccgct
      421 tattctgaaa aagtagacaa aaaagtggtc gccgatcgtt tagcggaatg gaaaaagaca
      481 aatgaacaag tcaagaaaac aactgtctct tctcaaaaaa acgagtcgac aggaacaagt
      541 ccttcagatt ctggcagtgg acaaacgata acgggaagtg aagaaaatca agcctcaatt
      601 attacgaatg agtcatcaca aggacaatat caatcctatg gacaatgaaa aacagaggtt
      661 gtgaaatcag cgttttgacc tgagtattag agaaacaaac ggcaaaatag ttcctcatat
      721 tttgccgttt tttgagctaa taccgaaggt cagctgattg aacaccgtgt atcaagaggt
      781 cttgagcctg ccgtttaact ctgagccaca aggagcaatt tcaaaaaata gctccttata
      841 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaccaaga
      901 ggttgtaaaa ttagcgtcct gacataatct aataccgagg gtcagctgat tgaacaccgt
      961 gtatcaagag gtcttgagcc tgccgtttaa ctctgagcca caaggaacaa tttcaaaaaa
     1021 cagcttctca tgttttataa atattgtgac ttgtggccga ggagttggca ggagaacacc
     1081 gtttaaggaa gaagttgtga aaaagctgtc ctgcatcaag aaataaggag ctgcgacaag
     1141 aattttgtca cagcctcttt ttttgtacga ataccgagaa actggctttt gaataccgtg
     1201 gagtgaagcg gtcaccacaa gacttttatc tcttcattag tttaatcgct tacatcgcag
     1261 cggttttatg ctgtgaaaag tggacggaat aatctttaga aagaataaaa aaatcttaag
     1321 ataacaactt ttttaggaaa ttttcatttt tcaggagcaa aataagaaaa aatgtgaata
     1381 gagagaagtg atatacgtga cacaacttaa aaaattgtgc caaaatcgtt tagcaatcgt
     1441 tttgacagct gttttctttt tctggctgaa gacgatcatt gcctactatg cagatttttc
     1501 attaggtgtg gaaggtacga tccagtactt tatcttgtgg atcaacccaa ttgcgactac
     1561 cttattgttt tttggccttt ctttgtatat taaaaagcca aagccagcgt tagctattct
     1621 tttgatcatt gatattttaa atacgctttt gttgtacttg aatattattt tttatcggga
     1681 attcacagat ttcattacag tcaaatctgt attaggcttt tcgaaagtgt cgcaaggatt
     1741 gtcaggaagc tccttttctt tgatgaagcc ccatgatgtc atctactggc tggatatcgc
     1801 tgtgttcatt gggctactgg tttggttgaa ggtcaagaaa atacctatca aaagcaaccc
     1861 tgtcggcaaa ccgatggcta tcgctgttac ctgtctttca gggcttattt tttcaggaaa
     1921 tctagcgctg agcgaagcca accgtccaca attgctgcag cgaacatttg accgttcata
     1981 tattgtcaaa tatctaggaa tcgatgcgta tactatttat gacggaatca aaacaggaat
     2041 gactagcagt gttcgcgcgc atgcaagcag taatgggatt gatgaagttt tagattatac
     2101 aaagaaacat tatgcagagc caaatcccga aacatttggg atcgctaaag ggaaaaatgt
     2161 gatcgttctc catttagaaa gtttccagca gtttttgatt aatatgaagg tagacggaca
     2221 agaagtcaca ccgtttttaa atagtatttt ccagaatcaa gcaacgatca gctttgacaa
     2281 tttctttcat gaagtaggac agggaaagac aagtgatgct gaaaatatgt tggaaactgg
     2341 cacatttggt ttacctcaag gctcattatt cacagaactt gggtcagaca atgtctttca
     2401 agccgcacca gcgatccttg gacaaaagca aggctataca agcgctgttt tccatgggaa
     2461 tgtagctagt ttttggaata gagatcatgt atataaaaac ttaggttatg acaatttctt
     2521 tgatcgatct tattttgatg aatccgacga aacgttaggt tatgggatat tagataaaga
     2581 tttattcaga gaatctgccc aatatcttga gcatctacaa cagccgtttt acacgaaatt
     2641 tctttccgta accaaccaca cgccttatta tacggatgac aagaatttcg actttccatc
     2701 tttgaatact ggcaatagca cagtagataa ttatgttcga acggcacatt atctggatca
     2761 atcattggaa caatttttta cgtatttgaa aaaatctggt atttaccaaa attcaatctt
     2821 tgtgatttac ggggatcatt ttgggatctc gaatacagat aataaagatt tagcaagcgc
     2881 tcttggtaaa gatccagata catgggatga atttgacaac gcacagatgc aacgagtgcc
     2941 attgatgatc catatgcctg gttatactaa agggacagtc aatcatgaat acggtggaga
     3001 aatcgatgtt ttgccgacat tactacatct attgggaatc gatgataaag attatcttca
     3061 ttttggtaca gacttattat cttctcagca tgatcaagta gttgcttttc gaaatgggaa
     3121 ctttgtgaca cccaaataca ccgtcctagg aggaaaagct tacaataatc agacaggaga
     3181 aatcatcgat acgaaagcag caggaatcga tgaggaaatc tccaaagatc aggaaaaagt
     3241 caagactgcg ttaagtcttt cagataagct gaatcaggaa aatctcttgc ggttttatgt
     3301 gccaaacgga tttgagacga ttgatccgaa aaaatatgac tataaaaatg aagcggaacg
     3361 taatgaagca atcgaaaagc aaaaaggcga acattcgaca agtgtctttt caaaaaatgg
     3421 taataaaaca acaaccaact tatatccagt aacaagaaca gacgttgaac agcaaaaaaa
     3481 taatcaataa acaatcaaac caaccttcag tagttagaca acattctaat gcttgaaggt
     3541 tggtattttt tatgaaaaaa acattttttt atttttttcg tgtatttttc ctttttaacg
     3601 tatctattta ttgaatgtgg aaaaatttaa attaaaaagg agaactatta tgtatgatgg
     3661 tgaattgatc aagaaacttc gagtcaacaa gaagttgaca cagtcccaat tagcggaagg
     3721 aatctgtttt aagacttctc tagtcgggat tgagacaaat tcagtgaaaa aaatgtcatt
     3781 tgtaacattg agatcatttt tagagcgaat gaacatgaca cttgcagaat atgaatggat
     3841 gcgaaaccag ctggatgaac caataaagtt aaagaaaaaa agacgaatgc tagatcgggt
     3901 acaagaagac agctttgatc cttataaaga gatcagcaat aaccgtaggc attttaagaa
     3961 aacagaagat ctgtattatt tggttttgaa tcttcacatg gtcgaaaaaa cagaagtatc
     4021 tgagacagaa tttgaatttt tacgttatga atgccgaatc attgaagaat acttgatgca
     4081 gataccagac tatggtcaat ttgaactatc tattctagct gattttcctt ggatattcag
     4141 tgatcggttc attgagaaca attatttgaa aatcaaaaaa agaatgcgac gattagcaca
     4201 gttgaacaat catgagcagt atctctttac ttttttaatg aacctgactt cgttttatat
     4261 agaaaatggt cggttgaaaa aggcacgtag tgtgaatgaa gatatgaaac ggagcttagc
     4321 acataaagaa agaagtacag tgatctatga aacattgatg gcagattatt atcaaagatt
     4381 gatttctgct gctttagggg aagaaaagac gaaagactac caagaattct tcacagtgct
     4441 cgaatatatg ttaggaaaga atcaacggag gtccttagag aaaaaactgc tggccattgc
     4501 tgaacccagc aactagacca gcagtttaga atttgtatca cttcgccgct ttttcagacg
     4561 gtttgtttat atcgaatggc gggaaaaagc gggcgatctc ttttaccgca gcatcacggg
     4621 aatcagaagc gtgaatgatg ttttcagtcc ccggcagcgc gtagtctcct cggatcgtac
     4681 ctggaacagc atcggcagct tttgttgctc caaccatctt acggacgata tcgatcactt
     4741 cctcgccgac cagcacaaga taaacgacag gaccggaagt catgtaatcg atcagttcat
     4801 caaaaaaact gcgctcagtc aagtgctgat aatgttcttt tgctaattca ggagtcatag
     4861 tttcaaactt catttcagca atggccagtc cctttgcttc aaaacgttgg atgatgcttc
     4921 cgactaaatg gcgtctcacg ccgtctggct tgataataac aagtgttctt tccataacag
     4981 cacctcgttt cctactatat ttctattata gcgggaggtg gaaaatgaga caaataaaac
     5041 cgcgtgagac aggaatataa cgtgtgtatt ttaaaaaaag tatgaaaaac atggaaaatt
     5101 gatttgacat cctctttttt agttgttata ttaacgaagg aaaaccgaat aagatttgtt
     5161 aggtgaggct cctatacaaa cataggctac tgcccaaaaa cgtcgagaga cgccaatggg
     5221 tagaacagga attgtcgaga taaggctttt cataaggtag ctaaaagcag gtatctgctt
     5281 tttacgttgt acagtgtcaa aactcaacga tgagatctga gaagcttttt ttaatatgct
     5341 ttttcaaacc tttcgctgag tatttggcga gaggtttttt tgttgccgca aatccccatc
     5401 ggtgaaaaat aaaaaggagg tggtctaatt ggaaaaataa ctattcgaaa aatgattgga
     5461 agtcatgaat taaaattact tagtaaagga gatgagaaca aatggatgac agtcattcga
     5521 aaaacaagga tctagaggaa aggccagggc tattatctat ctgttctcag cccctttcag
     5581 ttttttctct atcgatccga tgaatagcta aagaccaaaa gcagcgagaa cagctgtctt
     5641 ttggacacaa aggagagatt gagatggaaa aaaagaaaga tacacaaaaa atctatgcag
     5701 cttttgctgg aaaaacgaaa caagaattat ttgaactatt ccatgcatca gcaaatggat
     5761 tgacagaaga acaaatcgaa cgttcaagag aaaattacgg tgaaaatgcg atttcatatg
     5821 gcaaaaaaac accattcatt gtagaaatat tgaaagcata catcacgcca tttacattag
     5881 tgttgatcgc attaggaatt atttcattca tcacggaata tgtattagca gctcctggag
     5941 ataaagatct tttcggtgta gccattatct tcactatggt tatcgtcagc gggacaatga
     6001 cgttgattca gtccgtacgt tccaatcaag cggctgaaaa actgaaatca cttgtgaaag
     6061 taacggcagc tgtaaaacgt aatggaaagt acacagagat tcctatggaa gaaatcgtct
     6121 gtggagatct tgttcggctt tcagcagggg atatgatacc agcggatatg cgtttattgt
     6181 ccgcaaagga tttattcatt tcccaagcag cattgacagg ggaaagctat ccagtagaaa
     6241 agcatgcgaa agtatgtgaa gacgatcagc aaagcgaaac aagttacgaa aatcttgtct
     6301 ttatgggaag taatgtcatc agtggaagtg cggaaggaat cattgtttct gttggaaatg
     6361 aaacactatt tggtcatgta gcagaaacgc tatctgaaaa accaattcaa tcaagctttg
     6421 aattaggcat ccataaaaca tccatgctac tgatcaaatt tatgtcattg atggctccag
     6481 cagttatcgt catcaatggg cttacaaaag gggattggct agaagctttt ctttttggac
     6541 tatctgtagc agttggtcta acacctgaaa tgcttccaat gatcgttacg acgaatttgg
     6601 tcaaaggagc aagtgtgatg gccaaaaaag gaacagtgat caaaaatttg aatgccatcc
     6661 agaactttgg tgcaatcgat gtgttatgta cggataaaac ggggacattg acacaagata
     6721 aaatcattct tgaatatcat atggattgtg aaggaaatga agatgatcgt gttttgcgcc
     6781 atgcgtatct aaatagctac tatcaaacag ggctgaaaaa tttgttagat gtggctatca
     6841 ttgatgaagc aacagaaact ttagatacag ataagatcaa ttatcaaaaa gtggatgaaa
     6901 ttccttttga ttttgaacgt cgtcgtatga gtgttgtggt tgctgatcca gctggtaaaa
     6961 cacaaatgat cacaaaaggc gcgattgaag aaatgctgaa catttctaaa tttattgatc
     7021 ttggcggtac tgttacacca ttaacaaaag aacgaaaaga agccgtactt gcaaaagtac
     7081 agtcacttaa tgaagatggc ttacgagtga ttggtgtcgc acaaaagacc aatccaagcg
     7141 tcgttggaga attttcagtc aaagatgagt cagacatggt tttgattggt tatcttgctt
     7201 tccttgatcc accaaaagaa acaacgaaac aagcgttgaa agctctgaaa gaacatggtg
     7261 tagctgtaaa agttctgact ggggacaacg aattagttac acgttcagtg tgccgtcaag
     7321 taggtctaga gatcaatgaa ttggttaccg gtgagaaaat ctcggaaatg agtgatcatg
     7381 aattagcaca agtagctgaa gaccatgaag tattcgtaaa attgaatcct cagcaaaaag
     7441 cacgtttgac gacagcttta cgtaaaaatg gtcatactgt cggattttta ggagatggta
     7501 tcaatgacgc tcctgcaatg aaagtggctg atatcggtat ttctgtagat acagctgtgg
     7561 atattgcgaa agaatctgca gacgtgatcc ttttagaaaa agatttgacg attttggaaa
     7621 gaggccttct ttctggacga gaaacttttg ggaatatcat gaaatatatc aaggctacag
     7681 caagttctaa ttttggcaat atgttttccg tgctgattgc aagtacgttc ctgccatttt
     7741 taccaatgct gcctttacaa cttttgtttt tgaatctgat ctatgatgtt tcctgcattt
     7801 ccttgccatg ggatcggatg gataaagaat atttggacga gccaaaaaaa tggaatgcat
     7861 ctagtattgg ggaattcatg gtttactttg gtccaacaag ttcgatattt gatattacta
     7921 cttacttgtt gatgtatttt gttatttgtc ctgctgtagt gggcggaagt ttccatacat
     7981 tggatagttc acagcgagtg attttcatcg cattattcca tgcaggatgg tttgtcgaat
     8041 cactatggac acaaacctta gtgctccatg cactgcgtac accgaaagta ccatttatcc
     8101 aaagcaacgc aacatttgcc atgtttgtta tcactacatt aggaattatt gtcggttctg
     8161 tccttccttt tacaagtttt ggtgctgaat taggcttgat gccacttcct ggcaattact
     8221 ggttatggtt atttgctaca gtcatcgctt atctggcgct tgtgacagct gtgaagaaaa
     8281 tctatatccg tcgattccaa gaactcttat aaacgagaag gctggttctt tgcttctgtt
     8341 ctttttggac cagtcgtgct aaactatgtg tattgttttg gaaaggatta atgtatagat
     8401 gaaaggtgca atatttgatt tagatggttt gttagttgac acagaaaaaa cgtatcgtga
     8461 tggctggctt tggggatttc agcaatttga tatccatatc ccaaaagaaa tcgttgacgc
     8521 ttggggcgga aaaaactgga aacaaagttt tgatatttta gtgaaagcag caggtagccc
     8581 tgaaaaagta caggaagtcc gagcgaagcg tgaagaatat ttttatcaac agctgacaag
     8641 cggagccatc cacttaaaac catatgcaaa agaaatattg gcagacttaa aagaacgtca
     8701 tatgaaacta ggcattgcta cgaccactgt tacgaaaaga gcaacagata tcttggatca
     8761 ctttaaatta gctgattact tggacacgtt tacattcgga gacgaagtgt cggagaacaa
     8821 gccttcaccg attccttatc tgacagcttt ggaacgaaca ggattagaag ctagttcagc
     8881 ctttgcagtg gaagattctt tagtaggagc aacagctgct tcacgagcag gattaggcgt
     8941 tgttttgata cctgattcca gttttgtccg aaattatacc gtcgaagaaa aggaaaaatt
     9001 gaaacttctt gcggaaggaa aa
//
LOCUS       DAZULY010000120         8865 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000120 DAZULY010000000
VERSION     DAZULY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.121"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..283
                     /locus_tag="ACG81A_001805"
     CDS             <1..283
                     /locus_tag="ACG81A_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015165.1"
                     /translation="AADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQFIF
                     LFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            699..2237
                     /locus_tag="ACG81A_001806"
     CDS             699..2237
                     /locus_tag="ACG81A_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFS5015166.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            2234..3199
                     /locus_tag="ACG81A_001807"
     CDS             2234..3199
                     /locus_tag="ACG81A_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5015167.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3186..4508
                     /locus_tag="ACG81A_001808"
     CDS             3186..4508
                     /locus_tag="ACG81A_001808"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS5015168.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4571..4675
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4749..5492
                     /locus_tag="ACG81A_001809"
     CDS             4749..5492
                     /locus_tag="ACG81A_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5015169.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5507..6499
                     /locus_tag="ACG81A_001810"
     CDS             5507..6499
                     /locus_tag="ACG81A_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5015170.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6511..7248
                     /locus_tag="ACG81A_001811"
     CDS             6511..7248
                     /locus_tag="ACG81A_001811"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5015171.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            7238..7960
                     /locus_tag="ACG81A_001812"
     CDS             7238..7960
                     /locus_tag="ACG81A_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFS5015172.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8044..8667)
                     /locus_tag="ACG81A_001813"
     CDS             complement(8044..8667)
                     /locus_tag="ACG81A_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5015173.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2865 a         1518 c         1901 g         2581 t
ORIGIN      
        1 agcagcagac ttattgacaa ttcttacaaa atcacccaaa aaaacagagt atcaaggagt
       61 ttttttggct caagaacaaa aactggtgcc tgtgacaatg gtccagataa ataaagcaaa
      121 tcaatttatc tttttatttg aacacggcaa agcaccactt gctatgaaag acatctttct
      181 ttttttgatg aagaaccgtg agaaagctgt ttattatcag tcaaaagaag aactcattcc
      241 tctttatgga gtgaaagaga tagaaaataa agtggtgata taagtaaaaa taagaaaaca
      301 agacgtttat gatgtcaaaa caaccatttt ttacattttt ggcaaaaaag tgtcatgata
      361 cagtataata ttcgttgaac aggccaataa aggtgttctc ccccacgaga tttctacttt
      421 attggctgtt tctttttatt aaaataaaag ttgtgtgtgt aaaaaagata tagctgattc
      481 caagtgacaa tttcgtatag atttttatgg tattcgtagt gaaaaaacga gaaccaaaaa
      541 gttgatccga ctttgttgct tggattcttt ttttgtcagc atatttagag gtattttttt
      601 cttttttttg ttcgtgctgt aaggttaagg caagacaagt tagttgggaa aaaggaaaga
      661 aatgtttata cttaaagaat aacagaaagg aaggtcgcat gaataatttt atacaaacat
      721 taagtgaaag aaaaggcgag ttgctggaag ctgcatttca acatctgtcc atttctctca
      781 tttctttgct gattgcagca ctgattgcta ttcctttagc gatttgggca gccaatcata
      841 aaaaaatggc agaagtctta ttgcagatca cgggtgtgct ccaaacgatt ccttcacttg
      901 ccttattagg tttacttatc ccatttgtag ggatcggaac tgttccagca ttgattgcac
      961 ttgttattta cgctttgctt ccgattttcc aaaacacgta tattggtcta gctgaaattg
     1021 acccgtctat tgaagaagca gcggtagcat ttggtatgtc cagaatgaga cgcttgctga
     1081 aagtagagct tcccatcgca ttacctgtca ttatctcagg tatccgaact gccttagttt
     1141 taatcatcgg tacggctact ttggcagcat tgatcggagc aggtggatta gggacattta
     1201 ttttgttagg tattgaccga aatacacctg ttttgacatt gattggtgcg atcagttctg
     1261 ccttgcttgc aattatattc agcggactga ttcgctggtt gcagcataag aaacctcgat
     1321 attccatcat tactttagca gcagtggtgt gtttatttgg cggcgtttct ttatatcagt
     1381 ctgccttgtt taaaaatgaa accatcacga ttgctggaaa attaggagcg gaacctgaaa
     1441 tcctgatcca aatgtataaa gaattgattg aagacaacac aaaaacagaa gttgaattaa
     1501 aaccgaattt tggcaagacg agttttttgt tcaatgcact ggatcaaggt caaatcgata
     1561 tctatcctga gttcacaggt actgtcctag aaagcttggt gaaggtacct gaagcaatga
     1621 aaagtgctga tttgacaaaa gaaaaaacat atgacgaagc agaagaatta ttgaataagc
     1681 aattcgatat gacattatta aaaccaatgg cttaccaaaa tacgtatgcc ttggcagtca
     1741 agagatcttt tgcgaacgaa catcaactta aaaagatttc cgatttggca gctatctctg
     1801 atcagatcaa agcgggattc acactggaat ttattgatcg ttcagatgga tataaaggaa
     1861 tccaagaagc atatggcttg aatttttcaa gtgtacaaag catggagcca agtttgcgtt
     1921 accaagcaat caataatgga gatgtcaata ttgtggatgc ctattccaca gatagccagc
     1981 tcaaggagta cgatctagtg actctagaag atgatcgagg gttattccca gcttatcaag
     2041 gcgcaccgct gatgaaaaaa gattttgcta aagaacatcc tgaaatcgtt aaagcactga
     2101 ataaattaag cggaaaaata acggaggatc aaatgattga tatgaattac caagtcaatg
     2161 tcgaaaagaa aaagccgaca gaagtagcac gaatcttcct gaaaaatgaa ggattagtaa
     2221 aggaggaaga tcagtgacta aattaatcga gttcaaacat gtgcaaaaaa agtatgatgg
     2281 aaagtatgtc atcgatgacc taaatctggc aatcaaaaaa ggagaaatct ttgttttggt
     2341 tggtccttcc ggtagtggaa aaacaactac tttgaaaatg atcaatggat tatctaaacc
     2401 atcagcagga gacatctact tcaaaggaaa atcgttaaat gaatataatc tgcagaaaat
     2461 gcggtggaat atgggatatg tgctgcagca aatcgctttg ttcccgacca tgacagtcaa
     2521 gcaaaatatt gaagtcatcc ctgaaatgtt aggttgggaa aagcagaaaa gagcagaccg
     2581 tgtagatgaa cttcttcaaa aagttggctt atctcctgat atttatcgag atcgtatgcc
     2641 tagagagttg tctggaggag aacagcaacg gatcgggatc atccgagcta ttgctgcaag
     2701 cccagatgtg attttgatgg acgagccatt tagcgcatta gatccgattt caagaaactc
     2761 attacaagaa ttggttttgt ccttacacga agaattgggc acgaccatcg ttttcgttac
     2821 gcacaatatg gaagaagcaa tcaagctagg tgatcgtatt gcatttatga aggatggaga
     2881 aattatccaa tgtgacacac cagagcagtt attgatgaat cctaaaaacg actacgttcg
     2941 gcatttcttt gatgaaccga aacagacgaa agagtggcga gtagaagatt tagtcgtcaa
     3001 tggatatttc ttaaatgaaa taccagaaaa tgctcgtcaa caagtttgct ttgatacgcc
     3061 tatgaaagaa gtatattcgc ttctttcggt gtatccaagc attgtgatag tagaaaagca
     3121 aagaagtatt gggtcgctga caagtaaaga aatctttgct tttttaagtc gagaggagga
     3181 aggaaatgaa aacatatgat gcaatcatca tcggttcagg tgtgagcggc ttaagtgctg
     3241 cttatggatt gaaagaagca ggtaaaacag tactagtggt cgaagaagat ctatggggcg
     3301 ggacttgtcc taaccgtgga tgtgatccta aaaaagtctt gttaagtgca gtagaggcac
     3361 gtaatcgggt aaaacaattg agtggaaaag gttttaacga gatcccaact gccaactggg
     3421 aagaacttca aaaattcaaa cgaacattta cggatccagt cccagaaagc cgaaaaaaac
     3481 aattagcaga agctgaaatc gatcatcttt caggaaccgc tcgttttttg gatgattcat
     3541 ctattgaagt caacgaagaa gtcttccatg cggattactt agttttggca accgggcaaa
     3601 gacctacgat cttaccagtg gagggaaagg aatacttgaa aactagtgca gactttttat
     3661 cattgcctgt actgcctaaa gaaatcattt ttatcggtgg tggttatatt gcttttgagc
     3721 tagctacgat tgccaatgct gcagggagca aagtaaccat tgtccatcat aatcaacggc
     3781 cattaaaaga atttgaggcg tctttggtag aggaagccgt ccatcaaatg gaagcatctg
     3841 gcattcagtt tgcttttggt gttgaaaccc aaaagatcat ttcagaaggt actcgctatc
     3901 gtttagtcgg aaaagagacg gaattagtag cagatatgat tttttgtgcg actgggagac
     3961 agccgaatac agaatcttta gcacttgagc aagcgaatat cgtctttgat aaacacggaa
     4021 ttgcagtcaa tgattatctg caaacgagca atccaaaaat ttttgcgtgt ggagatattg
     4081 tttcaaggaa aaccccaaaa ttgacaccag tcgccacttt tgaaggaaat tatgtagcta
     4141 agcggataac ggatgcaaca agcgaaccta tcaaatatcc gattatacct acgatcgtct
     4201 acgccagtcc taaacttgct gaagtaggag ttacaaaaag tcacgcctct tcgtcagatc
     4261 aagtggtaga gatggatctt accagttggt tcacctatca tcgtgtcaat gaaccagttg
     4321 ctaaggctga attgaccttt gaccagcaaa attatttgat tggtgctgca gtgattagcg
     4381 agcaagcaga cgaattgatc gatgatttga cactcgtcat caatcaaaaa ttgactaaaa
     4441 aagagttgga tagctatatc atggggtatc cgacattggc aagtgatctc tcctatttat
     4501 tgaaataatc agcatgataa ctaaagcgct tgttgctttt tccttcttgt gctacacttt
     4561 aaaaaatgaa tacctgtttc tggggatgtc gtagacgaca tgagagtaag ttgtgaactt
     4621 agacccattg aacctgtcag ttaatactgg tgaagggaga aacatagcag acgcttgaag
     4681 cgggttacta tgtcgtgccc atttcaagcg ttttttgtta ttcatattag aagaaaggag
     4741 gtaacaaaat gaagaaaaat ctttatccag cagtcagtat ggcattcatt ttaattattt
     4801 ggcagattgt tgtcatagtt tggaaaatac ctgcctttat tttacccgga cctgctgctg
     4861 tattttctgc attagcggca gatgcacaag tattactttc ccattctgtc acgacattaa
     4921 atgaagcagt gcttggtttg gtaatagcta gcgttctttc ttttattact gccttggcaa
     4981 tggattattg gaagttttta gaaatgtctt tttatccgtt actagtgatt tcacaaatgt
     5041 tgccaatcat ggtgttaggg ccattactta ccctatggtt tggtttcggt atgcttccaa
     5101 aagtgatcct tgttgtcttg ataagctatt ttccgatcgt agtttctttt tcggatacat
     5161 tgaggaaagt atcaaaagag caaatcatct ttttgaaaac gatgggggca gatacaggga
     5221 agatttatcg aatatataaa ataccagcgg gtttatccgg cttcttttcc ggtttaaaag
     5281 tggctgctac ttattgtgtc agcggagcag tcgttggaga atggctaagc gctcaagcag
     5341 gcttagggta ttacatgata cgcgtaaaaa acagctacca gatcgataaa gtttttgctg
     5401 cgatcatttg tgtcattcta ttgagtttat tgctgaatgg cgcttgttca ttattgaaaa
     5461 aagggtatta tcgtatgctt tatatttttt aagagaaggg gatcaaatga aaaaattatg
     5521 gcttttatta ccgctactgt tgcttttatc cgcttgtggc actgcaaaag aaacaagtag
     5581 caaacaagaa ataaaggatt tgaaaaaagt gacacttgtt ttggattatg taccgaatac
     5641 taatcatact ggcatctacc tagcaaaaga aaaaggctat tataaagagg ctggtttaaa
     5701 tgtccaaatc atcgaaccag gagataattc gacaagtatt ggtctagtag gtgcggacaa
     5761 agcacaattt ggtgtaagtt atcaagaaga tgtcacttat gctcacgcag atggacagaa
     5821 tataccagtc aaagcaattg ctacagtgat caaacataat acttctggat tcgccaccct
     5881 atcagacagt aacatccatt caccaaaaga ttttgaagga aaaacatatg caggttggca
     5941 aagtccaagt gaagaagctg tcttaaaagc tgtgatggaa aaagacggcg gggacttttc
     6001 aaaactgaca atggtaggaa gcaatggaga agggccagaa agcttaggaa aatcctcaga
     6061 tatccaatgg tactttgaag gttgggatat gatcaaagca aaagaagcgg gtattgaact
     6121 aaactatatc ccattgaaag aattagatga acggttagat tactatactc cggtaattat
     6181 tacgaatgat caactgatca aaagtgatcc agaattggta cagtctttta tggatgccac
     6241 gaaaaaaggc tatcaggaag ctattaagga tccaaatgac agtgctaaac tgttgcaaaa
     6301 atatgcaaaa gaaaatgatc ggacgttttt agaagaatca caagcattct tgtcaaaaaa
     6361 ttatacggat gatcctgaga attggggatt gatggaagaa aaagtttggc gcaattatac
     6421 agcttttatg caggaaaacg gattgatcaa gcaagatgta ccttcgcaag aactattcac
     6481 taatcagttt ataaaatagg agtgatagat atgtcaatcc aaatcagcca cttgaatgta
     6541 gtcattgaaa acctgaaaat attagaagac atccatttca ctatccctaa agataccttt
     6601 acatcgattg tcgcgccaag cggagccggt aagtcaacat tgctgaaaac tttaacagga
     6661 gtccagccta tgacttctgg aacgatcaaa gtagacgatc aaaaagtaac tggtctccat
     6721 acagcattta gttatatgcc gcaagaagat atgttacttc cttggctgag cgtctatcaa
     6781 aacgttacgt tatatcaaaa aatcaatcat ctaacaatca atgaagaaca agtaagggat
     6841 tatctggata tctttgggtt aaacggctat gaacattttt tgccggaaca attatctggc
     6901 gggatgaagc agcgaacggc cttgttacga acaatcatga atccttcttc ttacttgctt
     6961 cttgatgaac catttggagc gttagatgct atgaccagag ggcagatgca agactggatg
     7021 ttgaagctgc ctaaaaaagc aaagcggact actttacttg tgacgcatga tatcgaagaa
     7081 gcaatttatt tatcagacag gattcttgtg ctttccgccc gtccggcaca tgtgattgct
     7141 gagattcaag tgcctgaaaa acgacggtca cgtgaatggc tactgcagca atcagagttg
     7201 aagcaaacga tttatcagtt attggcaggt gaattagatg ttaagtgatg ctcattgtca
     7261 tttagatggg agtcagagtt tagcactcct tcagcaggaa cacacgattt tgacgatcat
     7321 caattgtgat tcgccagaag aatggaagga aaacagacag cttgcagcaa gtaagactca
     7381 agccttaagt tacggtatcc atccttggaa agcagatagc tatacgtttg aacaagttga
     7441 gccttttttg aaaaaagcaa ggatcatcgg tgaaataggg ttagataata tttggacgaa
     7501 tgtaccgatg actacacaaa aaaaagtatt tgagcgacag cttgcttttg cagccataaa
     7561 tgagaaacct gttgtactgc acacaaaagg atgcgaaaaa gagatcttaa attatatcca
     7621 agcttaccct aatcactatt tgatccattg gtattcttct ttggactatc aacaagacta
     7681 tattgattta ggctgttatt tcagcattgg tcttgatttg aaaaagaatc cagcagtctg
     7741 gcaactagct aaagcagtcc caatcgacca cttacttatt gaaacggatg gactacatgc
     7801 agcagagtgg gctttagggg ggattttttc agaaaaagat tatgtctcac tgcttgaaga
     7861 acagttaaag acaatcgctg agatcaaaga gctatcagtt gaagaagtta aaaaaaatac
     7921 ttatcaaaac ttggaacgtt tcattagatt aggtaaataa aaaagagatg tgacacaact
     7981 agtgtcgcac ctctttttgg ctttctttgt ataaaagtaa actgtttgat taataaatga
     8041 atcttagtac caaccgtttg cttcccagaa tgctttcgcc gcttcccaag aaccataacg
     8101 tgaagtaaca tattgttctg ctactctttc ttggtttgca gcagagtagt caccatttaa
     8161 gtaagaagaa tctaattggt aacgtccgat gtaacgtccg ttcgttgctg tgtaagaacc
     8221 gcttgattct ttttgtgcga tccattcttt tgcagagctt gtgcttgctg gagtagcagc
     8281 agtttctgtt acagcaggag caggttgtgt ttcagcaaca ggcgctgctt gtttttccgt
     8341 ttgaaccgtt tcttgtacaa caggttgttc agcttggact ggtgcttctt gaacggctgc
     8401 ttgaggttca gcagctgctt gtgcagagcc ttctgttggg attgttaatt gttgtcctga
     8461 gtaaatcagg ttaatatcag aaattgaatt tgattctgca atggcattga tcaaggagtt
     8521 atcaccaacg tatttttgag aaatcgttga tagagtatca cctgattgaa cggtataagc
     8581 ttcgtccgca tgagcagtcg ttcccatgaa gaatgcagcg ccggcagcca aagttgttcc
     8641 aaaaagtaaa gttttaagtg atgtcatgaa aaatctcctt tattcgttaa aaagttttat
     8701 ttgttgtgtt caacgaggaa tactttaaca cgtgaaaata tttcataggt aaaagaagca
     8761 tgacaattta tgacaaatcc gcgttttttg taataaatac agtaatctat gaaatgaaaa
     8821 gggttacgtt taataaaact tgcttttttg tttttgttca ccata
//
LOCUS       DAZULY010000121         8851 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000121 DAZULY010000000
VERSION     DAZULY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.122"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..1387
                     /locus_tag="ACG81A_001814"
     CDS             47..1387
                     /locus_tag="ACG81A_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS5015174.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1605..2048
                     /locus_tag="ACG81A_001815"
     CDS             1605..2048
                     /locus_tag="ACG81A_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFS5015175.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2552..3970)
                     /gene="nhaC"
                     /locus_tag="ACG81A_001816"
     CDS             complement(2552..3970)
                     /gene="nhaC"
                     /locus_tag="ACG81A_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFS5015176.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4373..5596)
                     /locus_tag="ACG81A_001817"
     CDS             complement(4373..5596)
                     /locus_tag="ACG81A_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFS5015177.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5627..6466)
                     /locus_tag="ACG81A_001818"
     CDS             complement(5627..6466)
                     /locus_tag="ACG81A_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS5015178.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6483..7163)
                     /locus_tag="ACG81A_001819"
     CDS             complement(6483..7163)
                     /locus_tag="ACG81A_001819"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFS5015179.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7165..8238)
                     /locus_tag="ACG81A_001820"
     CDS             complement(7165..8238)
                     /locus_tag="ACG81A_001820"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFS5015180.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8294..8532)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8575..8808)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         2535 a         1812 c         1614 g         2890 t
ORIGIN      
        1 tcttaatata aattttgatt gtgaatttaa aagaaagagg tcatttatgg aagataaaaa
       61 attgaaaaaa caacttagct ccagacatat caccatgtta gctttaggtg gagcaatcgg
      121 tgcaggtcta tttaaaggaa gtggcgaagc gattgggatc gccggccctt ctgtattgat
      181 tgcttttttg attggtgggc tgattttata tatcgtgatg aaaggtctgg gaaaaatcgt
      241 tttaagcggt ggcgacacgc atcacggatt atctggtttg atacgccctt atctaggtgc
      301 tcattctgct gattttactg attgggtcta ctggtctatg tggattatca atatcatcgc
      361 tgaagctgtt gcagctgctt cctttttgca gttgtggttc ccgcacattc cagcctgggt
      421 atttgttttt atgttggctg ttttaactac gatcatcaat ctttattcgg tacggttatt
      481 cgcagaaaca gaatattggc tggcttttgc taaaatcagt gtcattatct tactgatcat
      541 ttttgctact tatcttgtcg gtcagcaaat gttgggcaca ggcgttttcc ctacccttca
      601 gcaattgact gaccatggtg ggtttactcc tcatggtatg aagggaatca tcagttcttt
      661 gcttgtagtt atctattctt acggcggttc tgaactgatt gctatcacgg taagcgaagc
      721 agatgatcct aaacgtgcga ttcctaaagc aatcaaaggt gtcatgggaa gaatcatttc
      781 cttttacatc attcctttgt ttttgctttt gattcttttt ccttggaata ctttagcagg
      841 cacgaatgtc agtccttttg tgatggtatt tgaaaaaatg aatatcccat ttgcagcgga
      901 tatcgtcaat tttgtgattg tccttgcttt gttctcatct atcaattcgg gtgtgtatgc
      961 atcttcaaga attttgttct tccgtttgaa ggatcgaaaa ggttcctcta gaaaactagc
     1021 tgttttaaac aagcatcaag taccgcaacg tgctgtttta ttttgtacca gtgttcttta
     1081 tcttggtgta gctctttctt attttgtcgg tgacaaatta tttggctatc tggcaggttc
     1141 cctttcgtat acagtattat tgatctggat cattatcagt gcggcatcct ttgtactggc
     1201 actaagaaaa ggaacattat gggataaagg aatcagcttt tttgcattag ctgtcttagg
     1261 attgattttc cttggtatcc tgttcacgaa ctccattggg gtaactgtat tgactggcct
     1321 gctttacctg ttcatttatt tcagttatca aaaaaagaat gatgcgttcg tattgacgaa
     1381 tgaatgaaat cgatagaaaa ttttatttta tttatgtgga atagatggag ctattgaaga
     1441 aaggagttgt gacaaaagtc ttgttgcaac tcctttttcc gaataaacag tctcatgacc
     1501 tcttggtaaa cggtgttcaa aaaccctctg tgcaaaataa ttgattgttc tccatgctca
     1561 tgacatgata gaagaaagga atgaaaaaaa ggagtttgtt ttaaatgaaa aagtattgga
     1621 agtttttgtt gatgatcggt gtttcaacgg tagttatgtt tggtatgatg tacttgaatg
     1681 tctatgcgct cgatcatatc ttcttcagtc gtacacgagt attcatggcg ttgatgatgg
     1741 gtgcagtcat ggcagtcatc atgctgttgt tcatgtggaa aatgtatgaa aacaaaaaga
     1801 taaatgttat gatcttagcc gctagtcttc ttgtttttgc cggttccttg tttatggtac
     1861 gaagccaaac tactgtcaac gacacagctt ggatgaaggc catgattcct catcattcta
     1921 tcgctatcct ggcaagtaaa cgagctgatt taaaagatcc tgaagtaaaa aaattagcgg
     1981 aagaaatcat tcaggcacaa gaagaagaga tcgacaaaat gaaaaaaatg atcaaagaaa
     2041 tggaatgacc attatccgtc caaataaaaa aagaggatgt tccagaagtt atcagcttca
     2101 agaaataaga agaagctact tctggaacgc cgtgaataag gggttgtgaa aaagctgccc
     2161 tgcatcaagt aataagaaca gccaaacaaa aatagcttct cctatttttc gtttggttag
     2221 gcttattacc gcagatgcaa gcttttgaac accgtttatt aagaggttgt aaaatcagcg
     2281 ttctgacctg agtattagag agacaaatgg aaaaatagtt cctcatattt tgccattttt
     2341 tgagctaata ccgagggtca gctgattgaa caccgtttat cgagaggttg tgaaaaagtc
     2401 gcttagtttc gcgtattaag aaaaattttg tgaaatggct ttccacattt ttgtataaat
     2461 ttggcttaat accgagaaac tggcttttga ataccgttta ttcgtaaaag ggattgtgac
     2521 aaaagtcttg tcacaatccc tttttatgtt tttattgttc taattgcgct gtcttgctta
     2581 ctgcttcttg cggtttcaca aatttttttg agattcccca acctgtgaag gcactgacca
     2641 tcgaaaacac aggagaaagc aaactgaaaa atacgaatgg caagtaagtg agtgtaggaa
     2701 taccaaatgt acttgctacg aaacttccgg caattcccca aggcactaag tagttgatta
     2761 ctgttccacc atcttctagc gttctaccta atacagttgg atcaaatcct gctttttcat
     2821 aagtttcttt aaatgcatta ccaggcagaa taacagatag gaattgttca ccaataaaaa
     2881 tatttactcc gatacttgtc atcaatgttg taaaaatcaa atttggtgtg ttcgtcatct
     2941 ttcttgatac tcgttccatc acggttgaga tcaagccaaa ttcgatcaac aatccaccta
     3001 atgacaaagt tagaataatc aatgacacag taggcatcat actttcgatt cctccgcggc
     3061 taagcagtga atcgatttgt tggtttccag tatttgaaac aaaccctgat tcgatgaccc
     3121 cagccaactt cgttaaggaa gtttgtgggt tttgaataaa aatcattcct actgctacag
     3181 cgatattcaa caaaatcgtt aaaattgccg gcaccttttt ccatgcacac agtaacatta
     3241 aaaataaagg aatcagtgac cagattgaaa tagtaaagtt tgcttctaga acatcagtca
     3301 cttcacgaat agatgaaaga cttgcggctt tacttgtgtg tcctaaaatc atgaataaaa
     3361 agaaagaaac aacaaatgcc gggacagttg accacatcat atgcttgatg tgtttgaaca
     3421 aatcggcatc aacaatagct gctgctagat ttgttgattc ggataatgga gacatcttat
     3481 ctccaaaaac ggcacctgaa acaatagctc caacaaccag cgctggattt aagcctagtg
     3541 ttgtcccaat accgaaaaat gcaatcccaa tcgttgacat aacagtaaag gcactcccta
     3601 cagcgctacc tacgattgca cacacaacaa atacagaagg gacaaaccac tggacactga
     3661 tcaatttgaa cccaatgacc attagagttg gaatgatccc tgcttgtatc catacagcaa
     3721 tcaatgaacc gactaaaata aaaataaaga ttgggatgat tcctggtttg atgccattga
     3781 tgattccttc gtgcagccaa tctgtcgggt atctttttat cactgcatac aacatgacca
     3841 ttccgatcgc tgctaagatc gtgatattag gtgagacctt taatccgaca acgccaatcg
     3901 caatgcctaa aacaataacc attaatacaa gtgttgcttc taaaaaactt ggtgttcctt
     3961 tttttcccat gataatctct ccttttttag ttgttcttat gttctaaaaa agttaagata
     4021 tcggattact ccgccgcaat tcatccgcga ttcctccttt ataaaaataa aaagacctgt
     4081 tttaaactaa ggacgaatga ctccgcggta ccaccttaat taaaaacaga tctgtttttc
     4141 actttcaaac ttttattcaa atgtatggac tttccatgta attcgtcaat taggcttgat
     4201 cggttcacag cgaccaccga ctttctgaac aaaacaccta accgctactg cgtgaaaagt
     4261 tgacttgtac gaagtttacc ttccataaga tcatttgtca atgaaaaaga aaatattttt
     4321 tcatttttct gaaaattatt tttataactt tcacacacaa gtttattttt tactagaaag
     4381 tagcgccctc ttgttttttt cttgctacta tttcttgatg ttcttgcttg attctttcta
     4441 atagctttgg ctctttcaac agatcaagag ctgtattggc gagtagttct gctgcaatcg
     4501 caatagaagc aaggcctttg tcacttttag cagcagcttt gaattcttct gtgtgtccgg
     4561 caatatatgt ctcagagatt gctaccgtag gctggattgt tggtatgact tggctgacat
     4621 ttcctacatc agaagaaccg agacttgttc tttgttccgt ttctatttct tcatctggaa
     4681 cacctgcttc ttttgcatgc tggaagaata agtcatcaaa agaaggagta acgatgacat
     4741 catcaactgc attttggaac aatccaaact caaaagtcgt gcccgtactt atcgcagcac
     4801 ctcgtacgat attttccact ttttcatata ctttatctaa tgtttgccga tttcctgcac
     4861 gtaaatagaa acgtccagca gcgtaatctg gaatgacgtt ggcagctact cctccgtctg
     4921 taatgatccc atggatattg acatcttttg gcaattgcaa gcgtagagcg ttgattccat
     4981 taaacacttg gatcaatgct tccaaagcat ttactccttt ttcaggtgct gctgctgcat
     5041 gggaagcgac accgtaaaat ttgatgtcga ctggatcatt cgctaaagat tctgtcgttt
     5101 tcccataacg aaaagctgga tggacgcata acgctacatc gacatcctca aaaaaacctt
     5161 ctcgaacaaa gctgccttta gctgatccgt tctcgccccc ttcttcccct ggggtgccat
     5221 atacacggat ttctccacca gtttcatcaa tgaattgctt cactatgctg gcggctaata
     5281 cggagtaagt accaaacaaa ttatgcccgc atgcatgacc aatacctggt aaagcatcga
     5341 attctgccaa aaaggcaatt gtgggaccgg gtttattgga aatataacgt gcatcaaagg
     5401 ctgttcggtg tccagctact tctttttcta cccaaaacct ttctttttct aattgtttaa
     5461 tcaacgctgc tgaggaataa aactcatagt tacttacttc aggatgttcg tggatatcta
     5521 gagcaagtgc ttgatattct ggtaaacgat attggattag atccttcaat tggtttgttg
     5581 agattgttgt tgtcataaaa aattcctcct atgctagtca tttttcttat tttctcccga
     5641 atgtttccca tgcaggcacg cttgatcctt ttgatgtttt ctcgattact ttgactgtgt
     5701 cttctgtttg ataagcgtct actatttttt gatacgtttt attgttttca tcttcttttc
     5761 ttgcaacgat gatattcaca taaggacgtg catggtcatc cactggttct aagaagatgg
     5821 catcagaagc aggattaaat ccagcatcta cagctacacc gctattgatc actgaaatgt
     5881 ctacatctga caaagcacgt gctgtttggg aagcatctaa ttcagaaatg tctagatttt
     5941 ttctattctc ggtaatatca gaaacagtgg gcgcttgtcc ttttgcctta tccactttga
     6001 tcaatccagc agtttgcaat aacagaagag ctctgccgcc atttgtaaca tcattaggga
     6061 ttgctacttt tgctccgtct ttgatatcag ctacatcctt caaagtgtta gaataaattc
     6121 ccagcggtgc attgacagta tttccgatcg atactaaatc agttccgtgt tctttattgt
     6181 aattgtctaa aaagaattga tgttggaaag aattcaaatc aatcgatcca tctgctaaag
     6241 ccacatttgg ttgattgtaa tctgtaaatt tcacgtattc caaattaatg ttctcctttt
     6301 tcaaacggtc tttcacgttg tcccatacat ctgtatcttc tccaataatc cctagtttta
     6361 cgatagtttc ttttttgtca gttgtatctg cttttccttg gctacaccct gccaaccaaa
     6421 taattgctac aaatgtaatg actacaaaac gaataatttt tttcattttt tctcctccac
     6481 tttcaatgtg taattttttt aatcagttga ttactgataa attgacttaa aaaaactaag
     6541 attagaatca ataatgtagc cacaatcgtt acatctgttt ggaatcgatt ataccctctc
     6601 gatatagcaa gattccctaa tccgcctgca cctactgctc cagccatggt agtcagtccg
     6661 atcacgttga tgatagtcaa cgccgagacc cgaatgatac caggcaaacc ttctaacaaa
     6721 tacacacgta aaatgatacc gattggactg gttcccatcg cttctgccgc ttcgatcacc
     6781 ccaggatcaa cttctagcag tgcgttctcg atttgacgag caaaaaacgg aacaactcca
     6841 aaaatcaatg gcactaaagc tgctttcgca ccaatcgtcg ttccaactaa gaaacgagta
     6901 gtcaatgcta aaagagaaag caagataatg aaagggatgg aacgaatgat attgatcagc
     6961 ttatcaagta tttcaaatag cagacggtta gctagtaatc cattagggcg tgtcactact
     7021 aacgtcacac ctaagatgat tccaatcatc ccgccaatga atgctgtcca aaaagacata
     7081 tatagtgttt gcttcgtcgc ttcgacaaat tcatccggaa tcaaataaac attgggcaaa
     7141 tatgttttaa agaaatcagt cattttatct tcctccttcg atcactttga tacgactatc
     7201 taaatcgttt ttttgtaaaa tatttatttt tacaccagat ttgatcaaat aatccaatgc
     7261 ttttttccgc tgtttggttt ctccagctaa agtaacgatc aaactcccta acggcgtttc
     7321 ttgtaataac tctacattgc catataaaat attcgctgtt acttgatatt tgctgtacag
     7381 atgagcgatt agaggttcgt tcgtctgatt tccaatataa cttaattcca ccagccattt
     7441 attggcgatc tgatcggcaa aagcagaatg ggcaatgatc gtttctaatg cttggtcaag
     7501 atgagtagct gttcgaataa aatctttggt caattcttct tcaggattgc tgaagatctg
     7561 gatgctgcta cctttttcaa cgattcttcc atcttccata accgctacct tgttgcagat
     7621 ttctttgaca acttgcatct catgagtgat caggacgatt gttaaaccta actggcgatt
     7681 caattttttt aacaatgcaa ggatctgaag ggtcgttttc ggatcaagtg cactggttgc
     7741 ttcatcacat aataatacct taggttcatt cgccaaagct cgtgcgattg ctactcgttg
     7801 tttttgtccc cccgacagtt gtttaggata agcagatgct ttttcttcca aaccgacaag
     7861 ttccagtagt tcattcactt tttgacgacg ttcctgtttc gattttccag aatactttaa
     7921 agaaaaatca acgttgtcaa aaatagttcg gctttccatt agattgaaat gctgaaagat
     7981 cataccgatg gcctttcttt cttttcgcaa ttctttggga gagagctgac tcagattcgt
     8041 ctgattgaca atgacttctc ctgctgttgg tttctgcagc aaattcatca cacgaactaa
     8101 cgtacttttc cctgcacctg agtaaccgac gattccataa acatcgcctt tttcaatcgt
     8161 taaatctaca gaatctactg cctgtatctc tttttctttt tgtttgaaac atacagaaac
     8221 atctcgcaat tcaatcatta aaattcctcc ttatctaaaa aaagcgtcct caagaaaatg
     8281 aaaatgtcat tttcttgagg acgctttttt gcgtgttacc acctcatgtt caagctgtgt
     8341 tcacacacaa ctcctcttca agtacgatca tactctagca agatatcggt cgcagccgtc
     8401 atcgccttac atctatgctc gggatgaatt gctcgatggc cattttccaa tagtcgttcc
     8461 ttgcttcttc tcagctaccg aagctctctg tcaaaggttc ttctatcgta cttaccattt
     8521 caatgcaaat aatattcaaa aaaaaagtcc ttttactaac ataaactatg tcagtaaaag
     8581 gacgacgtat cgcggtacca ccttttttcg gaagtttcct tccctcaagc ccaacatctc
     8641 aaaaagagag attggcgttc gttttaacgg tcgaacagtt ccgaatttgc ctacttatcg
     8701 acaaattatt ctccaaggcc attttcagct tttcttactt gactcttctc accagccaga
     8761 gcctctctgc aaagtttcca aacttacttt ccttttcaac gaaatcgata ttttgttgtt
     8821 gggattaatc tacatgagga aaactgaaaa g
//
LOCUS       DAZULY010000122         8830 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000122 DAZULY010000000
VERSION     DAZULY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.123"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            164..1033
                     /locus_tag="ACG81A_001821"
     CDS             164..1033
                     /locus_tag="ACG81A_001821"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFS5015181.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            1198..2469
                     /locus_tag="ACG81A_001822"
     CDS             1198..2469
                     /locus_tag="ACG81A_001822"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS5015182.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            2466..3761
                     /gene="rho"
                     /locus_tag="ACG81A_001823"
     CDS             2466..3761
                     /gene="rho"
                     /locus_tag="ACG81A_001823"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFS5015183.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            3860..4123
                     /locus_tag="ACG81A_001824"
     CDS             3860..4123
                     /locus_tag="ACG81A_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFS5015184.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            4568..5017
                     /locus_tag="ACG81A_001825"
     CDS             4568..5017
                     /locus_tag="ACG81A_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5015185.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            5074..6492
                     /locus_tag="ACG81A_001826"
     CDS             5074..6492
                     /locus_tag="ACG81A_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS5015186.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            6492..7352
                     /locus_tag="ACG81A_001827"
     CDS             6492..7352
                     /locus_tag="ACG81A_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS5015187.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            7526..8299
                     /locus_tag="ACG81A_001828"
     CDS             7526..8299
                     /locus_tag="ACG81A_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5015188.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            8446..8811
                     /locus_tag="ACG81A_001829"
     CDS             8446..8811
                     /locus_tag="ACG81A_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFS5015189.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
BASE COUNT         2908 a         1537 c         1953 g         2432 t
ORIGIN      
        1 ttaaagcgct attggatatt ttattttttg caaaaaactt cttgaaaatt ttcattttca
       61 ttgattttag tagaaaacta ccgaaatgtt tggtaaaata gggtcgttgg ttaagaattg
      121 tcttaacaaa tttagtttta caaaacttag gaggaatttt attatgccag tagtatcagg
      181 agctgaattt ttaaaagctg ctcgtaaagg cggttacgct gtcggcggat tcaacacaaa
      241 caacttggaa tggactcaag caatccttga agcggctgaa gcaaaacaag caccagtact
      301 tatccaaaca tcaatgggtg cagctaaata catgggtggt tacaaaatct gtaaagattt
      361 aatcactgat ttagttgaag caatgaacat cactgttcca gttgctatcc acttagacca
      421 tggtgattac gaagctgctt tggaatgtat cgaattaggc tatacttcag taatgttcga
      481 tggttcacat ctaccatttg aagaaaactt gaaattagct aaagacgttg ttgaaaaagc
      541 acatgcaaaa ggtatctcag ttgaatgtga agttggttca atcggcggag aagaagacgg
      601 aatcatcggt aacggcgaat tagctgatat tgaagaatgc aaacaaatgg ttgctacagg
      661 tatcgactac ttagcttgcg gtatcggtaa catccatggt caatatccag aaaactggaa
      721 aggattagct tttgaccact tacaagcaat tgctgatgct gtaggtccag acatgccttt
      781 agtattacat ggcggatcag gtattcctca agatcaaatc gaaaaagcta tctcaatggg
      841 tatctcaaaa gtaaatgtta acactgaatt ccaattatca tttgctgcag ctactcgtaa
      901 atatatcgaa gctggtaaag ataaagaagg taaaggattc gaccctcgta aattattagc
      961 tccaggtaaa gcagcaatcg ttgaagatgc taaatctcat atcgactggt tcggttcagc
     1021 taacaaagcg taagcatcca cataacagat acgatatata ggaatggaga acgacttttg
     1081 aagtcgctct ccattctttt ttacttaaaa cggcagacca tgctcgtttc ttttttttat
     1141 ctatgataaa atacaagcat atccatgaaa aaagattaat aaacaaggtg gaaacagatg
     1201 aaaaaaatcg taatcaaagg aaaccgacct ttagctggtg aagtaacgat cagcggtgca
     1261 aaaaatagtg cagtcgcatt gattccagct gcaatcttag ctgattcccc agtgatcttg
     1321 gaaggtgtgc cggacataca agatgttcat tcattaattg aaattttaga gatcatgggc
     1381 gcaactgtcc atttttccaa caacatacta gaaatcgatc ctcgagaagt cgtatcgatt
     1441 ccgatgccgc acggaaaaat caatagtttg cgtgcttctt attattttat gggaacactg
     1501 cttggtaaat ttggcgaagc ggtagtaggc ctgccaggtg gctgttattt aggccctaga
     1561 ccgattgacc tacacgtcaa aggatttgaa gctctaggtg ctgaagtgac aaatgaacat
     1621 ggtgccatgt atttacggac agaaaataaa ggattgcgtg gcaatcggat tttcatggac
     1681 gtggtttcag tcggagcaac gatcaatgtg atgttagctg ctgtaaaagc aaaaggacag
     1741 acagtaattg aaaacgctgc acgagaaccg gaaatcatag atgtggcaac tttactgaat
     1801 aatatggggg caaaagttcg aggagcgggt acagatgtga tccgtatcga aggagtcgaa
     1861 acattacatg gctgtcggca tttcatgatt cctgaccgca tcgaagcagg aacctatcta
     1921 gcattagcag ctgcagtagg aaatggtatc aaagtgaaaa atgtgatctt tgaacatttg
     1981 gaaagcttta tagcaaaatt gcaggaaatt ggtgtccata tgaaaatcag cgaagatgaa
     2041 atcgaagttt acccctcgaa agaattaaaa cccgcaaaca tcatgaccta tccttatcca
     2101 ggatttgcca cagatctgca gcagccactt actgcgttac tattgatgac tacaggcact
     2161 tcagaaatca tcgatacgat ctatgcgaaa cgtgtgaacc atgtgccgga actagcacga
     2221 atgggcgctg atattaccgt ggaaggaaat atgatcatcg tgaatggtcc aaataaactt
     2281 cacggcacag aagtcgtggc ttctgatctt cgggcaggtg cttgtttagt gattgctgga
     2341 ctattggcag aagggacaac gacgatttac aatgtagatt atatcttacg cggttatgat
     2401 catattatcg agaaactaac agccttaggg gcatcaattg aaatgataga ggaagtggga
     2461 gctgaatgag cgattatttg acaatggcag aactagagaa caaaacgtta aaagaagttt
     2521 atagttatgc caaagaattt aaaattcctt attacagtaa gatgaataaa aaggaattat
     2581 ctctagctgt gattcgtgca caagcagaga aacaaggatt ttattatatg gaaggaatat
     2641 tggatatcgt tggtcaagat ggttacggtt ttttacggcc aatcaattat ggaccaagtg
     2701 cagaagatat ctatatttct gcctcacaaa tcagacgatt cagtttgaga aatggggaca
     2761 aagtggcagg aaaagcccgt ccacctaaag aatcggaacg ttattatggt ttgatgcacg
     2821 tggaaagcgt taatggaaaa gatccggaag aagcaaaaga acgtccgcat tttccagcct
     2881 taacacctct ttatccgcaa agacagcttc aactggaaac aacaccgcaa agactatcta
     2941 cccgaatgat cgatatcttt tctccaatcg gttttggtca gcgtggattg atcgtcgcac
     3001 cgccaaaagc agggaaaact tctgtattaa aagaaatcgc aaatggtatc accgaaaact
     3061 atccggaagt agaattaatc gttctactga ttgatgaacg accggaagaa gtaacggatc
     3121 tagaacgaag cgtgaaaggc gacgtggtat cttctacatt tgatctgcag ccgcaaaacc
     3181 atactcgagt ttctgaatta gtattggaac gcgcaatgcg tttagtcgaa gataaacgag
     3241 atgtcgttat cttgatggat agtattacta gacttgctcg ggcctataac ttagtcgttc
     3301 caccaagcgg acgtacattg agtggcggga ttgacccagc cgcattgtat aaacctaaga
     3361 aatttttcgg ggcagcaaga aacatcgaag aaggtggcag tttgacgatt ttagcgaccg
     3421 ctttagtcga tacaggaagc cgtatggatg acgtgatcta tgaagaattc aaaggaactg
     3481 gaaatctaga attacaactt tcacgtgaat tatctgaacg tcggatcttc ccagccatcg
     3541 atatcaaaaa atcaggtacc cgaaaagaag aattattgat gccaagtgaa gaattagaag
     3601 aaatttggaa acttcgaaaa aatatgactg gagattcatt agaatatacg gaacaactga
     3661 tccgcttttt gcgtaaaaca aaaaacaatc agcaattttt caaagaattt caagatgtct
     3721 cctttggtaa aaaaaatccg accagaaatc tgaaaagata attgcaacct tgactgaaac
     3781 atgttaagat gttaatgttg taaatcgaca aatcaactct ggttcagcaa atggagcagg
     3841 gcaaaggagc gaaaaaatca tgaaagaaaa catccatcca gattaccacc cagtagtatt
     3901 catggattca acaactggtt tcaaattctt gtcaggttca acaaaaactt cacaagaaac
     3961 agttgaatgg gaagacggta atacataccc agtcatccgt gtcgaagtaa cttctgactc
     4021 acatccattc tacactggac gtcaaaaatt cacacaagca gacggacgtg tggaccgttt
     4081 caacaaaaaa tacggtctca aagacgcaaa cgctgcggaa taataaaaac gctgataact
     4141 caacgtttag cagactatct gatagggata gtctgttttt ttatgttttt tgccaattta
     4201 aaacttcgtt tgatgctgaa aaagtcgtag gatcataatg cacaaaacaa tgaaaaacta
     4261 gtatatagtt ttaattatcc acttgataaa aacaccttat attaacgaaa tgaagagggg
     4321 ttatttcgaa gtaaaaaacc atttacctaa aattagaagc taagctttcc tttcaaggaa
     4381 ggctttttgt tttgttatag acttgcgatt gtttgtgagc tagatcgttc attttaatga
     4441 cttatacacg cggatgatgg gtctttttcc tctgttcatt cataatattg aacggtacag
     4501 ctagcgatgc tatactgacc tcagttccaa gaaaacgtta acattttttg tgggggagag
     4561 gttttttatg aggtttgatc aattgatcgc agaagaactg attgttttcg atccatcgat
     4621 agattcaaaa ggatcattat ttgagcgaat aggggatgtg ttgaaaaaaa caaataaggt
     4681 aagaaatgtt caaaaagtcg ttaaggcact attcaaacga gaggaggaga tctccacagg
     4741 aatagaagat ggatttggga ttccccatgc aaaaagcaaa tatgtgacaa aaccagcact
     4801 gtgttttttt catacagcca aattaacgga ctatagcggg atagatgaaa tgccaattga
     4861 atatattttt gccatcgtag ttcctgaaaa atcagcagat atgcatctag agattctcag
     4921 cacactctcc agacgattga tgaatgcaga ttttagaaag ggaataaaag aagccactac
     4981 gccagcagat atcatcagac agattagtca agaatgaaaa tagtagagtc tttgtccgag
     5041 tatcgatagt gaagaaaaag gaggaaaata atcatgaaaa tcgttggtgt aactgcttgc
     5101 ccgactggaa ttgctcatac ttatatgtcg gcagaaaaat tagaagtaac agcaagaaat
     5161 ctaaaccatc aagcaaaatt cgaaacacaa ggtgtaaaaa cagaaaatca gttatctgaa
     5221 caagaaatca aagaggcaga tgcaatcatt cttgcagtag ataaagaaat cgaattggac
     5281 cgatttgccg ggaaaaaagt aaagcgtgtc tctacaagcc gagcaatcaa agaaccgcaa
     5341 gtagtaatag acgaagcatt gagagatatt ggcgtattcg ttgtgtctaa tgaaaaagaa
     5401 ccagctgcaa atgagaaaaa gccgacaatc tataatcatt tcatgaatgg tgtgaattac
     5461 atgcttccat ttgtgatcgc aggcggaatc atcatcgcac tgagttttgc ctttgggatt
     5521 acagcggctg atcccaactc agcagactat aatgtgttag cagctgcatt ttcaaggatc
     5581 ggaggagata cagcgtttgc catgatggtt cctgcgttag gcgcggggat tgctacatcg
     5641 attgctggaa aagcaggttt tgccccagga attgttgccg gattactggc ctctacaggt
     5701 ggttcaggtt ttcttggcgg aatgattggt ggcgtgctag cagggtatat ctgtgatttt
     5761 ctggcaaata aaattagcgt aaaaaaagaa ttttctgcta tttatcagct gattgtcgtc
     5821 ccctttttat cgatcttaat tattgggctt ttgatggtat ttgtgattga acaaccgatc
     5881 acatgggtgt tggatgcttt gacaaattgg ctgaatagct tgggaaatac atccgggcta
     5941 ttgttcggat tacttatcgg gatcatgatg gcagctgata tgggtgggcc gatcaataaa
     6001 tcgacttcga ctttctcgat cggtttgatg tcagctggag taaatgcacc gattgctgct
     6061 tgtatggctg caggaatgac acctccccta ggtattgctt tagccacttt attattcaaa
     6121 cataaattta ccaaagaaga acggacttca ggtcaatcat gctggatttt aggcgcttcg
     6181 tatattacag aaggtgctat accgtttgcg gtatctgacc cgatccgcgt gatacctagt
     6241 ttgatgacag gttcagcagt cgctgcaggg atttccatgg cagtaggagt tacttcaatg
     6301 gcacctcatg gagggatttg ggtgatgcta attccaaatg ttatgaacaa tttacctatg
     6361 tatgtcttgg caattgcatc aggtacagtc actacagcct ttatgcttgg actattgaaa
     6421 aaaacagtga aagagaaaaa acaagacaag tggacgaaaa atgaaaatct aacaaatgag
     6481 gtggaagcgt aatgttatat acaatgaaag agttattgaa agtggctaaa gagaatcaat
     6541 ttgcagtacc agccttcaat atttgcagct atgatatgat gaaagcaatc atcgaagaag
     6601 cagagagatt agaagcacca gtaatcttag aaattcatcc tgatgaaatc gcctatttaa
     6661 gtgatgagtt tgttgcggca gtaagagcct atgcttatac aagtaaactg ccgatcgtta
     6721 tccacttgga tcatggcggt acgatagaag atgtcttacg ggcgattcga aacggctatt
     6781 cttccgtgat gatcgatgcg tctttgaaat cattcgacga caatgtacag attacgaaaa
     6841 aagtggttgc gttagctcat gcggtaaatg tctcagtgga ggctgagctt ggaacgattg
     6901 ggaataatgg ttctgctgaa ggaggtgctg aaacaatcat ttatacagat cctgatcaag
     6961 cgaagatttt tgtgaaagaa acaggaatcg atacacttgc tgtagcaatt ggaacagcgc
     7021 atggcttgta tccgaaaaac agaactccaa aattaaatat cgacttactg aaagaattga
     7081 accaacaaat cgatcttcca tttgttttac atggcggttc aggtaatccg gacgcagaag
     7141 tcagcgaagc agtaaaatac ggagttgcca aagtgaattt gagctcagat cttaaaagcg
     7201 tctttttcaa tgctttgaga gaaatattga gtgaaaatcc atcgatgtac gagccgaatg
     7261 caatttatcc agcagctaat aaaaaggtac aagaagtggt caaacataaa atggaaattt
     7321 tgaacacagt aggcaaggct agtttatact aaaaataaaa atgtgtggtc aagagggtat
     7381 gacaatagtc ttgtcatacc ctcttaacca cacacgatgt tcagaagtag ttttttcgaa
     7441 aaatttcttc ttatttctcg aagccaatca cttctgtcac aacctctttt ttcctgttac
     7501 tatataaagt aaatggggtg attttatgtt gaatgaagtg gagaatagac agcaggagat
     7561 tgtgaattat ctaaaaaaca accaatttgc aagagtcacc gatttgatcg agttagtaaa
     7621 ttatagcgaa gccacgatta aacgtgacct tgtttttctg gaaaaaagtg gattgatccg
     7681 tagagttcgc ggtggcgcca tgcttattga caatcgaaaa attgacgtgc cttacctgat
     7741 gaagatcacc aaattagaag aagaaacaga aaaacgtaag attgcagata cagcttcttc
     7801 tttgattcga gatgatatgg tcttattctt ggattccagt acaacctctt tacacttagt
     7861 cagaaaccta gcaagatttg atggtttgca gatcattacg aatggcatcg tcacagctgc
     7921 tatgttgagt gaatatacaa cggcaaaagt atctgtattg ggaggggcaa ttgttccgaa
     7981 acgagcaaca atcaacggtt caaaagccta taacgatgca ttgtcatatt atgcggattt
     8041 agcgataatg agttgtcgcg ggctagattt cagccaagga gtaacagaaa ctcatgaagg
     8101 agaggcaatg gtcaaaaaag cttttagaaa acaatcagac cggcttgccg ttttagttac
     8161 acagcaaaaa attgcacata aattcatgca tcaagccatt gcttttcatg aaatcgatta
     8221 tctggtgaca agtaaagaac tgacaaaaga acagtatgcg aaagtaaagg aacaccatat
     8281 cgaatgtctg tacggataaa ttattacgtg aatgttctaa gtggcttcct catttttaga
     8341 taggacctta tctttgctcg cttctctcag ccgatggtat aataatgaaa aggttatctt
     8401 gttagataat cttacttttt tatccaggag aaaggagtgc agaaaatgaa acatgtgaag
     8461 gcgttggtag tgaaggcaat catgatctgg gcgattctgt gggtcgtgct gacaggttta
     8521 tacggtgtta gctttatgga ttcaacgatt gttggggtaa ttattgtagt catgatctat
     8581 gtgctagggg atctgttgat cttacgtaaa gtagggaaca tcgcagcgac gatcgcagat
     8641 gctggttcag ctgcagttat cttgtggctc tatctatatt ttatgaatga tacagtcgat
     8701 atttggatga aagtattgat tcctgctcta ttgatcggtg tggcagaatg gttcttccac
     8761 aagtggttgc taagtcaagg catcgtacca gatgaacgga aaatggaata aatcaacagt
     8821 taaactattc
//
LOCUS       DAZULY010000123         8811 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000123 DAZULY010000000
VERSION     DAZULY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.124"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..714)
                     /locus_tag="ACG81A_001830"
     CDS             complement(52..714)
                     /locus_tag="ACG81A_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5015190.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            898..2544
                     /locus_tag="ACG81A_001831"
     CDS             898..2544
                     /locus_tag="ACG81A_001831"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS5015191.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            2535..3617
                     /gene="uxuA"
                     /locus_tag="ACG81A_001832"
     CDS             2535..3617
                     /gene="uxuA"
                     /locus_tag="ACG81A_001832"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS5015192.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(3725..5278)
                     /locus_tag="ACG81A_001833"
                     /pseudo
     CDS             complement(3725..5278)
                     /locus_tag="ACG81A_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            5426..6567
                     /locus_tag="ACG81A_001834"
                     /pseudo
     CDS             5426..6567
                     /locus_tag="ACG81A_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(6693..7772)
                     /locus_tag="ACG81A_001835"
     CDS             complement(6693..7772)
                     /locus_tag="ACG81A_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS5015193.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(7875..8315)
                     /locus_tag="ACG81A_001836"
     CDS             complement(7875..8315)
                     /locus_tag="ACG81A_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5015194.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(8372..8782)
                     /locus_tag="ACG81A_001837"
     CDS             complement(8372..8782)
                     /locus_tag="ACG81A_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS5015195.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
BASE COUNT         3011 a         1570 c         1616 g         2614 t
ORIGIN      
        1 tactcaaaaa cgaaggttaa ttataaaaat agaagaggtc gcaagaaaat gctactgctg
       61 gaaatactcg ggataacgtt gagtaacagt ttcctgttct ttcagcatca tgtgtaagtg
      121 caatcccgtt tgaaatcgag cttcttctgg ttttttatca actaaagcct tgagtaaatc
      181 ataatgttgt tccatgatcg tttcccatgg aagcccctga atggtcactc gtaaccagcg
      241 gaaacgttct aaatgggtat tatggtcatc taaccagttc caaacttctt ttcttcctgc
      301 aatttcgaaa caagcttcat gaaataaatt atctgcacgg aaaaaagcgc gttcgtcatt
      361 tattttgact gctttttctt gttcttctaa aatcgtttct agcactattc gtgtttgtgt
      421 gtccaatttg gaacaacatt caatcatgat ttgctcttcc aactgttctc ggacaaagcg
      481 agcattttct gcagaaacaa gattaatttt ggaaacatac gtaccacttt gaggtatcgt
      541 atacacgagt tcttgctgac gtagttgaat caatgcttcg cgaatgggag tccggccgat
      601 agcaagatgt tcctccagtg ccttttcaga tattttcctt ccaggttcta agtcagcata
      661 gataatttgt ttacgaatcg cttgataagc ttgtgattgc aagctgcttt tcatctttat
      721 tcctccaaaa tcatttctat gaacgtagta taacagaaga ggtctgaaaa acaatctggt
      781 atgtcacatt aactagtaaa tttgtataaa tagtaaaatt ttggttgaca tagcgatttt
      841 tattgcataa aattcacttg aatacaaaaa ctaatatgtt agtttaggag gggaaaaatg
      901 attgatttaa acaactggag gaactttatc cctgaattat ctcaaaaaga agtagaaata
      961 cctcattttg accgtgcatc attaagagaa aatggacaaa aaaatcctgt ctggatccat
     1021 ttcggtggcg gaaatcttta tcgaggattt catgcagaaa tcgctcaacg tttagcggat
     1081 ctaggcgaat tgtccactgg tgtcattgtt tgtgaaacat tcgatgaaga aataattgaa
     1141 agggcttacc aagattttga tgatttgatt cttgaagtgg tcatgccaag cagtggagag
     1201 ttcgtcaaac gtatattagc tgcgacagct gacagtctgt attgtcatcc agattgttct
     1261 gaacactttg ataagatgat tcgacacttt gaaaatccta gtttacaatt agttactttt
     1321 gctattactg aaaaaggtta tcgattgaaa gatacaaata ataaatatat aggaccgatt
     1381 gcagtcgata taaaaaatgg tccaaagaag ccatcacata caatgagtat tcttgctgtg
     1441 ctactttata aacgtttttt atctggagct tggccgattg cacttgtctc tacagataat
     1501 ttttcacaaa acggtagtca attacgtact tctttgctaa cgatcgcaaa agaatggcaa
     1561 aaggaaggtt atgtacaaaa tacatttatc caatacttat cgaatgaaga aattgttgcg
     1621 tttccatgga cgatgattga tcgtatcacc ccgaatcctt ccttgtcagt tagcaaaatg
     1681 ttggcaaaag agggcttttc aaatatgtcg attttccaaa ccgcaaaagg aaataatatt
     1741 gctccatttg tcaatacaga ggacatccat tatttaatca tggaagatca ttttcccaat
     1801 ggacgtccga aattcgaaaa agctggggta ttcttgactg atcgtgcaac ggtagataaa
     1861 gcagatacga tgaaggtgac gacttgcctt aatcctttgc atacgtcttt agctattatg
     1921 ggttgtttac tggggtatag gagcatagct gaggagatga aagatccaga tttatcgctg
     1981 ctagtttctg aaatcgggta caatgaaggc ctcccagttg tggagtctcc aggtatcatt
     2041 gatccaaaga tctttctgga agaagtaatt actgtgcgct tttctaatcc aaatattcct
     2101 aatatgcctc agcgaatcgc cacggatacc tctcaaaagg tggcgattcg ttatggtgag
     2161 acgattaaaa aatatatgga aatagaagtt aaagacccgc agacacttgt attcatacca
     2221 ttgactctcg ctgcttggct tcgatacctt attggcattg atgatcaagg aaaaccattc
     2281 atcccgagtc cagacccact attgacagac cttcagcata agctttccga tattcaactg
     2341 ggtacgcaag atacatcaca tattcatgag aaaatggctc ctattttgtc aaataaagaa
     2401 atttttggtg tagatcttta ccaagcagag cttggcagaa aaatagaaga aatgttcatc
     2461 aagatggtag caggaccaaa tgccgtacga aaaacattac aggattactt acaagaacat
     2521 ggaggaaatt tatcatggaa atgactttta gatggtacgg atcacaggaa gaaaagatta
     2581 ccttagatga aatcagacag attcctgcgg tcaaagggat tgtaggcaca ttgatggata
     2641 taccagtagg agaggtatgg cctagggaga ggatcaaaga actaaaagaa gaaattgagt
     2701 ctcgtgggtt aacactaaaa gtaattgaaa gcgtcaatgt gcatgatgcc attaaaattg
     2761 gattaccttc ccgtgagcag tatatcgaaa actataagca gacaatccga aacttggcag
     2821 aatatggaat agaagtcatc tgctataatt ttatgcctgt atttgactgg gtgaaatctg
     2881 accttgacta ccgcttagaa gacggctcat ccacgttagc ttttatatct gatgatattc
     2941 cagatgatcc aagagaaatc gtcgcgcgca tcgaacattc cagcaatgaa tttggattgc
     3001 cgggatggga acctgagcgc ttagctccta tcaaatcctt gcttgaagcc tatgcatcag
     3061 tagacgaaga aaaacttcga gaaaattttg cctattttct ccaatcaatc atcccaacat
     3121 gtgaagaggt aggggtcaaa atggctgtac atccagatga tccgccatac tcgatttttg
     3181 gattagctcg aatcgtaaag aatcgagaag atttagattg gttgtgtcgc atagtagaca
     3241 gcccttataa cggaatcact ctttgtacag ggagtattgc agaagatcca gaaaatgatg
     3301 tatatgggat tttagcagaa ttttgccaaa gagatcgaat cccatttgca catgttcgaa
     3361 atattaaatt tttagctaat cgagatttct atgaggcacc acataaatcc gaatacggtt
     3421 cattagacat gtaccgcata ctaaaaacaa tgtatgacaa cggatttaac ggatacatcc
     3481 gtcctgatca tggaagaatg atctggaatg agacaggaag accaggatac ggcttatatg
     3541 atcgagcatt aggtgctgtt tatctaaatg gattatggga agcaatcgaa aaaggaaaca
     3601 aaagtaaaga taattagtga aaaaaactgt aattttatta tgtaaataag aaggatgttc
     3661 aaattcttga tacataaatc cggaggttgt taataagcat tcggatttat gtatcttatt
     3721 tggtctagga agtaacttga ttttcttttt tcgattgatc catgagtgct tcttctttta
     3781 attcaaagat atattctgag atcctcataa tcactttttt ccagtcttct ggtgtgatga
     3841 ttccttttaa aatatagatt tttttgatat gagaaggcaa ttgtttcaaa gggcagtcgc
     3901 tgataacgat gttgcagttc tccataaaat cattttgtat tttgatcgat tcatttgaaa
     3961 aaatttgttc tatcttaacg atcagatctt tactgatata aaaatcacgc gtatagttca
     4021 caccaatcac aattcgcggc tgttttttgt agcgatcata caaatgtact aggtcttcaa
     4081 ccatccattg cacattttcc ttttctaaaa taactggtaa caagtgttga ttttgttctc
     4141 tgaaatccat ataaaattgt tcaatggcgg tttggacttc tttcaatacg ctatgttctt
     4201 gataaagcat gaaagtagga aggggcgtat ttggttgatt gaagttaaga aatccattat
     4261 aaagtttatt tcgtgcgaat gccaatgagt aaagaatctt ttctttttca ggtataaaca
     4321 aatcaaatac agtacaaagg gactccacca tttctcttga ataaagaaca tgaggaacgt
     4381 ttgctttttt taaaagtttg tattgctcca agcttgtttc ttccgattcg attttcgtga
     4441 tagaaagtcg agctaaaata ttcactaaga tgcgttgttc gcgacaaatt ggcagattag
     4501 gtcgcaaaat atctacagag agatcaataa aaaagtcaat atattcatac tctgtgaatt
     4561 ctttttgtac gtctttcaca gaagttgcag gaaaattctc tttacatatt tttaatagaa
     4621 aatataattt atctatcata ccgctattta gtcggtcaag tacttctttt ttaaaaaaag
     4681 tattcatcat aaaagccact acttgcggat cttcatcata aaattctgta gcagtacttg
     4741 aaaagatgtt ccaataaata tctaatgctg catattgaat ttgtacctct tttccagaaa
     4801 aagactttct gccattatta gaaaaggaga tagacaaacc gtactatgct aaaaatgcat
     4861 taatcttttt cattttcgta tacaaataag aggcactata gtttgtttgc tgactgaaac
     4921 tgataatcga atttgtctga cagctgaaca aagaagtcaa gatctgataa ctgactgatt
     4981 cgttgcagta tcttccaaat agacgataat agcagtaagc cggatcaatt ccttcaaaat
     5041 tcaactttaa aaaaggtttt tgcttatcaa tatgcagttc tgttagattg agacgctcaa
     5101 agtcttgttc taattctttg atgtatagta aaaaatttga ttcagacata tggaaatgat
     5161 ctgctaaata ttgtgtagta ataaagaaac gatgttcaga taggaaaaga aaaatatcta
     5221 attttgtatt gctttttttc ttcaagataa acgttttaat cgcttcttga gtttgcatga
     5281 aagaggctcc tttttactta aattgttaaa acttgaaaat attactgttt ttttcataaa
     5341 tttagacaaa aaaacagtaa acatataggt agaatctcta ctatcttatt tcttgcatac
     5401 aaaaaaatca aggagtgaga gagttatgag atattcaacc atcttaatat caacatttgc
     5461 agccttaagc ttatcgctta ctataaatca gacagtccac acagaggaga cagaaacagc
     5521 caaatggaca gaaaccaagt gggccaacgt caatcgaatt acctttcagg atgaattttc
     5581 atccttcaat tcaggagtcg caaaattaag aaaaggacaa acagcgacca tcaaagcaaa
     5641 aattgactat tcgggagact tagcacctat actacaatca gctgttacat ttgagaatgt
     5701 tgatcccact ttgtcgattg gctatgacga gaacactttg actatccaac acaagcaagc
     5761 gatatttacc ttcaccgtta cattaaatca ggacttgtta aaaccagcgc tttttactgt
     5821 caaggtatca gatggactac cgaacgatca tcatttaacg ccgtatagtc agcggcagac
     5881 aattgagcaa gattcagcca tcgactcagg aggagatctt gttgaagaac caacagataa
     5941 accagaaaat gaaaataagc cagaagtacc gcctacagaa aatccagacg gagaacagaa
     6001 accggaaata gaacctggag aagaacccga tacagaaact caaccagagc cagacaataa
     6061 atcaaaacct gaaataaccc ctggagaaaa accagatgta actccagaac cagatacgga
     6121 ttctggaaac caaacagtac cggaaacaaa tccagacaca gataatgaaa ctgaaaatcc
     6181 agaaaaacca gaggtagatc cagaagaaaa accagatgtg actccagaac cagatacgga
     6241 cgcaagagac caaggaatac cagaaaaaat aaacaaaaaa acaattcaag aggatggaaa
     6301 aaaagagagt aaaaaatcaa atctagcaat tctaaaaata aatgaagagc agctgaataa
     6361 aaaatcacga atatttgata gcgcccaatc agctgaaacc ttaaaaagta gcaaggatac
     6421 aacctttgct gtcctgaaac gaaaaataaa cagctaccaa aatccggaga gtcacaaaac
     6481 aaagttatac tgtggagcgg aatcattcta ttaagtatag caacaatgct ttctgcaaaa
     6541 agatttaaac aaaacagatc tctctaacat ttgaataatg tctgtgaacg catttttcaa
     6601 ataaaaagaa atatccagaa aatgacccca aaataccaga ttgaaatctg atatttgggg
     6661 tcgttcatac tttatctttc ttcatattta ttttaaggga tatctgtttc ttttggaaga
     6721 tggttcggga tctttttcac tttcaataca tctaaaaaga aggtaaagat cggaatgccg
     6781 acaatcaagc cccaaacgcc aaagaaccgt tcgctaacaa gtaagaccac aaatgtataa
     6841 aaaatcggca gttctgtttt gctggacatc aacttcggat tcagcacata agactcaatt
     6901 agatggacaa tcaaaatcgt aatcaggata taaacaacat ctcggatgcc tccttgggaa
     6961 taagctatga aagatagagg aatacaagaa atgataacac ctgctactgg gatcagactt
     7021 aaaatgaaaa tcatgatcgc taaactcagc agctgatgaa agccaaatac agccaaggcg
     7081 attgtagtga gcaacgtatt taaaagagcg acaacaaatt gagcttctaa taccaaacca
     7141 aatgtattga caaacttatc ggcaaaatag taaatatctt gaaagaacca agaaaattca
     7201 ctttttaaaa acaatcttga gaatacaatt gtttttttct tttcaatcat aaaaaagaaa
     7261 cttaagatga atgacatagc aaacgccact gccatttttc ctatatcctg taaatagcca
     7321 agcgcaatcg aagcgccatt ttgaagctgt tcaaaaaaat cgtttcgctc aatataagta
     7381 tggatatatt gtaaaacagg atctgcatct tttggctgat gctcgtaaaa attaataacg
     7441 gactggatca tgtcaaatgt ttgatgaata agcatcggga tatactttgt gaatgccaga
     7501 tagataaggt aaacgatcag tgtataaatc atgatagcta aaacacctgt agggattcgg
     7561 acaaatcgct gaatgatttg aacaagatga atcatcaaat aagtaaagat aaatgtgagc
     7621 aaaatggttg taatcatact tctaactaag aaaagaacaa aaacgacaaa taataaaaca
     7681 gtgagacggc gtattttttc attcgccaaa aaacgctgat aaacggtcaa atcgacaacc
     7741 tccaaataac aaaaaataaa cacgttattc attatatgat ggaatatgaa aataagaaag
     7801 agcaaaaaga aaaaccgcta aatgacgtta tcttatgagt tggttttcct cataaaatcg
     7861 ttcatctagc agtttcagcc ctttttctgg tcttgatgaa aattatctag catttttcgg
     7921 gtcagatgtt gcagtgtatc caattcttcc tgttcaagtg gaagataaga cttgatcttt
     7981 tgtggaatac cttctgcttg tttttgcaac gcagcacctt gagcagtcag cgtaacattt
     8041 acgattcttt catcacccgt ttcacgctga cgattaatat aatttttttc tgctaacttt
     8101 tttagtaagg gagtcagcgt accagaatcc agacacaaat catttcctag ctctttcact
     8161 gaagtctttt tcttttccca taaaactaac atcacaagat actgggtata ggtaagattc
     8221 agcggcttga ggaggggagt gtagtgtcta atgatttctt tagaggcggc atataaagga
     8281 aaacatactt gattaactaa atatagatct tccatttttc cacctccgct gttttactat
     8341 agtgtatcat agcggattta cacaaaaaag tctattcact aactgctttc agagtaactt
     8401 ctatatttcc agctgtagct tttgaatacg gacacacttt atgcgctgcc tctaataatt
     8461 cttgcgtttc ttccagagaa agaccttcga tagatccttc aatctctaca cctaagacaa
     8521 agctcgtttc actttctgaa taaagggaaa cttgtgcact gacagtagaa gccccagaga
     8581 tatttttttg ctgtttgatc aattctaatg cactgttaaa acatgcactg aaaccagctg
     8641 caaacaattg ttcagggttg gttgcatttt cgacgcgttt accaggagct gcaatattca
     8701 attcgaatgt acgatcggga ttatgcactt ctccagagcg tccgcctgtg ttgatcattt
     8761 tcgtagaata catttttttc ataaaattac cacctttaat tatattttat a
//
LOCUS       DAZULY010000124         8799 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000124 DAZULY010000000
VERSION     DAZULY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.125"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..825)
                     /locus_tag="ACG81A_001838"
     CDS             complement(<1..825)
                     /locus_tag="ACG81A_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HFS5015196.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQT"
     gene            complement(831..1937)
                     /locus_tag="ACG81A_001839"
     CDS             complement(831..1937)
                     /locus_tag="ACG81A_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFS5015197.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            complement(1937..3073)
                     /locus_tag="ACG81A_001840"
     CDS             complement(1937..3073)
                     /locus_tag="ACG81A_001840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS5015198.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(3105..4556)
                     /locus_tag="ACG81A_001841"
     CDS             complement(3105..4556)
                     /locus_tag="ACG81A_001841"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS5015199.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(4600..5364)
                     /locus_tag="ACG81A_001842"
     CDS             complement(4600..5364)
                     /locus_tag="ACG81A_001842"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFS5015200.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(5392..6090)
                     /locus_tag="ACG81A_001843"
     CDS             complement(5392..6090)
                     /locus_tag="ACG81A_001843"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFS5015201.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(6102..6881)
                     /locus_tag="ACG81A_001844"
     CDS             complement(6102..6881)
                     /locus_tag="ACG81A_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFS5015202.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(6897..7841)
                     /locus_tag="ACG81A_001845"
     CDS             complement(6897..7841)
                     /locus_tag="ACG81A_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS5015203.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7887..8666)
                     /locus_tag="ACG81A_001846"
     CDS             complement(7887..8666)
                     /locus_tag="ACG81A_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015204.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2491 a         1840 c         1308 g         3160 t
ORIGIN      
        1 tgtctgtgct atttcatgat ttattctctg tgctaaaaca atcatataat ctatcatcaa
       61 ataatccacc atcatatcat tatttttcca ataatttaaa aaaaagtctc taattatagc
      121 aaacatccat ctagactctt gattacatgc taatgaataa cccgcaaaat atcctgatgc
      181 tacggatgcg tgagcataat caggcctcct aatagaccaa atatcgatat caaaatactc
      241 atctggtatt ggttttgtac aataaaatgt ggcgtctaac cacaaacctc catgttttgc
      301 tagcaaagat aatcttaata aatcagaata atttgttctc gtaatgattc ctttgttttt
      361 cttttcttcc acccattttg gtatatcaac atattgtcta taattatctt ctgttaaaat
      421 tataacagta tgtttccctg catttcgttt tattgatttt acacaagctt ttacaatttc
      481 cggcgattga tccaatccct gccaccaaca aacccaaatg cagttagaat atggtgattt
      541 tgattgatta attatttgat tttgactata atcatatgtt ttcaaaaaat ctgaaaaagt
      601 cttttcataa tacttcagca ttacctcatg tttttttaat aaatgttcct tctgtttttt
      661 actttcatta tatccattac gattcatgac ttgaatatca attttagctt gtaacgtggc
      721 aaatgcttcc ttccaagatg ttatctttgc aattcgtctt gttgcattca attcttcgga
      781 tatgcgatgt ataattcttt ttatacttga atgtttctgt gtcatattct ttaacctctt
      841 tgtattaatt caaataactc ataatatttt gattctaaga ttttgtatgc tgtttgaata
      901 ttataatctg cctttattaa aatcgaacgt tctgaatctc gatgagagat atctacattt
      961 gcaatctctc tcgcccattc ttttgccgat tgatttaaac ttatgaaatt ggtcttatca
     1021 ctgaaagcaa cttctgtagg aactttctcc gaaaagaaac acggtaaatc tgcaaattga
     1081 gcttcaatac ccactaacgg aaccccttca aataatgatg gcaacattaa tacgtcaaat
     1141 gcttgataaa atttttctat atcatttcta tttcctaaaa atttaatatc atcatctatg
     1201 ccacttttct ttgcataatt ttttatttct tcttccatat caccagttcc aataattaat
     1261 aatacggaat tattcttaat agcgtgaaat tctttaaaaa tttcaatcaa aaaacgatga
     1321 tttttagaat atgaaattct tccaatatgt cccattacaa acgtctgact tgaaattcct
     1381 aatttcattc gtacatcatt tctaatactt tcatcataat aaaatttttt agcatccact
     1441 gcatttggaa tcaaagtgaa tttatcattt cgaaacaaaa aatcacctgc tacttttcca
     1501 catgaaaatt catgtgtcaa aacagacgtt aacttacatc gataatattg ctttaaaaga
     1561 tatttaaaat ctctatctat cgatgtatta tggctatgtg ctatccgtat cggaatccca
     1621 gctttttttg cagcttttaa aggtaaataa ctcatcgcat caatatgaga atgaacaatc
     1681 tcatattctg gatgatcctt aaaaaattgt tccatccatt taaaatattt aggataattt
     1741 tgtggataaa gtctcggtgc ataatagacc tttcctccaa gacttattat ttcctcatca
     1801 taatcacttt tatatggacg atgtgtcaaa aaatcaaact gaattttagt tcgatctata
     1861 ttcctataat agttcattag catcgtctcc aaccctgcac gatgcatatc attcacacac
     1921 tgtaatattc gaatcattat atatttcccc tatacaaatt taatgtttct tgtacgattt
     1981 tatcccaatt atatttacta caaataaact cagtagcttg attcttcaaa actttaacca
     2041 tttccgattg attgcatgcc tcttctaatc ttatctttaa atcacttaca tccgatttct
     2101 taaaaatcat agccttgtct tctactactt ccgtacactc tgagatattt gaaactatac
     2161 aacagttccc ataactcatt gcttctaaca aacttaatgg cattccttct aaatcacttg
     2221 gaagtgtata gatatacgca ttactatata attcttctaa ttcctgtcct tgtacaaatc
     2281 cagtaaatat aattcgctca tcaccttttg ctaattcctt tagttcattt gcaaattcat
     2341 ctgtatcaga gcttcctccg gctattataa gttttttatc tgtctgaaca tctttaaatg
     2401 cctcaattag ataccgaatt cctttttcgg gaaccaatcg ccccaaaaac aaaatataag
     2461 aatcttttgt taatccatac ttttctgtaa tcaatcctgc tgattttatt tcaatttttt
     2521 taacgccatt tggtataaac cttgtttttc ttccatatat atcttcaaaa taattttgga
     2581 cgctttcact aagtacaatt atttcatctg caaattttac agcacacttt tcccctaaca
     2641 tgatgtatgt actagcaagc ttgttccatt tttctctttg atgatctaga ccatgaattg
     2701 ttgcaacaca tcttttccca aaaagttttg gcaaccacaa catagcacat ggtccttctg
     2761 catgaaaatg aacaacatca tattttccga atgctgctct aattccacca aatactgaag
     2821 aactcattgc tgcaattcct tttatattta atgtaaaaat agtctttaat tttacacctt
     2881 tatattcttt atttttatgc tggtcaaatt cctttccgct aacatgatga ccacttcgat
     2941 tatagcaagt cacttcatgc ccaagtgcaa ccattcgcgt tgccaattct tctaccacta
     3001 tctcaattcc accttctctt gatggaattc ttttatgacc tagcatacag atttttaatt
     3061 tttttcttgc catcacgttt tactccttct atagtatctt tatcttacat ggaaccgttt
     3121 ctatgaagca cagttttgac tgtcctaaac aaaattctaa aatctaatcc catactccaa
     3181 ttcctgatat attctgcatc aagttttaca acctcttcga agtctgtgat atcacttcta
     3241 ccactcacct gccacattcc agtaatccca ggtttcatcg caattctcgc acgatgatga
     3301 aaatcatact tctcatattc tgacttcaag caaggtctaa accccaccag gctcattttt
     3361 cccattagga cattataaaa ttgtggaaac tcatccaatg atgtagatcg aataaattgt
     3421 ccaattcctg tcttctttgt tccatcagga agtattttat tgccaatgat tcttgggtca
     3481 aaatccatct taaacatgag attattactc atcttatttt ctttcatcaa ctctgctttg
     3541 cgttcttctg catccatata catactacgg aacttataca tcttaaatgt cttaccattt
     3601 tgaccaatac gttcttgtgc aaagaaaatc ggccctggtg atttgatgta aataactggt
     3661 gctacgaaga taaaaataac acctgttgca atacatccaa taattccagc aagaatgtca
     3721 atcgttctct ttataaatgc ttgcctcatc gtcataacat taatactaga tgtaataaca
     3781 gtataatcac caactcggcc aataaattgt tttccacgtg tactatcaga cacttttgta
     3841 agattcatat gtacagcaag tcccatttcc gaacatctgg cgattaattc tttgggatat
     3901 ggaacacttt cgtcaacatt gatgaatact tcatctaccc aattctgtaa aagatattcc
     3961 gctgcattat cagcatttgc aactactggg acacctccga tggtagttcc atacatatct
     4021 ctgtctacta caatcacacc actaagttta tacatgccat aattcttctc tttaagatta
     4081 cgaactacat tttttgcaat cttttgagat gttacaataa ctaacgatac atcttcaccg
     4141 tttttcatgc ggtgatgcaa caaccctttc caaagtatac gaattatata tgttaataat
     4201 ccatacacca cccccattat aaataaagaa actctggaat aatcatttcc atcctgcatt
     4261 gtaataaggt ataaactgca cacaagtaac agcataaaag attgctttat agaagctaca
     4321 gcctctttat aatatcctct ttttaaaaca tttttatatg cctcaaataa aaatataatc
     4381 gtaagattct caagttctat aaaaattgcc atgtggcgat aaataggaat cacatacgga
     4441 ttccagtcac cctgtcgcat tacatatgat atataaaaag caagctggac acaaaataag
     4501 tctaataaaa taaaatcaat atgtttagac caaccacttg agttttttct atacatctct
     4561 cactctctcc tcatttcctt tttatctacg aataaagaac taaaataaac caaacttctt
     4621 cttttttacc tcttcataga ttggataatt gatctcatcc ccgttgatca gatcctttgc
     4681 gacttgtttc atctgtacta ctttttcctt accaaactcc ttggcaatct gctcatagca
     4741 ctcttttaag taaaaggtgc gcttagagac cccgtgtgca tcagaagcag ccatctgcac
     4801 gagattatgt ttgaccattg ttttggctgt tttctggata tctttgccaa atacaccaac
     4861 ataacttggc gcagtaagtt gggccaaaca tcccatatct aggaacggaa gcaaatgatt
     4921 tgggtcttct cgaaaatgcg cgttgcgttc tggatggacg atcactggta tctttcctag
     4981 ttctaacaac tcaaaaaaaa gccgttctgc atataacggc acttccagtg tcggaaattc
     5041 gatcagaaga tatgtatcat ctaaatctgt aaataaaatt tcatctcggt ggatgtcttc
     5101 gatgagcgtt ccggtaatac gtacttcttg tccttccaat aacgtcaaag gcaactgtct
     5161 tttctctaat tcggcttgta acgaagcgac tagagaaatg acttgtgatt tttcattgct
     5221 gtattttcca ttattgtggt gtggtgtgca taaaatatgc gtaatgcctt gctgaatcgc
     5281 tttttcagcc attgcaatac ttgcttctaa gttttctgcc ccgtcatcga ttcctggtag
     5341 gatatggcaa tgtagatcga tcatttctct tgtctcctct cttcttattc cttaatttcc
     5401 gtagtaataa ccagcatctt tgctgtgttc tgctccgttg tatacgacac ctaagactcg
     5461 tgcttgtacc atatctaaca gttcttttgc tttagtcaat gattcttttc gtgacgtatt
     5521 ttcccgtacg accaataagg tgccatccgt ttttgaagac atgatctgtg catctgttac
     5581 ggcaacgact ggcggcatgt cgaagatcac tacatcaaat agttgtcttg cttctgctaa
     5641 aatctgatcc attcttggcg aacttagtag ttctgatgga tttggtggtt taggtccact
     5701 tggcagaatc gacaaattgt cgactggtgt tcgttggatc tcatctgcta cacttcctga
     5761 tgaactaaga acagtactta atccgcttgc gttgttcaac ttgaacgttt tatagatgac
     5821 gggcttacgt aagtctgcat cgactagcag cacccgttga ccagatttgg caaaaacaac
     5881 ggcaatgttt gctgcagtgg tcgatttccc ttctccaggt cctgatgaag tcacaacaat
     5941 cgttttgatt tgctgaccag ctgcggaggc aaactggata ttcgtccgaa tcgtacgata
     6001 ttgttcagcg attggtgaag agggattggt gagtgtgacc aaactgactg catgagtttg
     6061 ggctgctttt tgtttctgtg ttcgtgccat tttctttcct cctagactct tgagcggctt
     6121 ctgcgtgatg gcgctgtttt atcgtttgta gcttttggtt gtgtctgagc tggtcctttt
     6181 ttacggatac cagcattcaa ttctttcgct gacatttctg gaactgttcc taagattggg
     6241 aatcctaatg tatctgtcac atatttttca tctttcactg tccgatcgag taattccaat
     6301 aagaatgcca gacctacacc taccatcatt cctaacatta agccagtcat taaattcaat
     6361 ttattatttg gcgaaacagg tgtcgtattg gcaactgcac ctgaaatgat cgagatttta
     6421 tcaacattca tcacatcttt tgcgttctct tggaaaactt gtgaagttgt gttagcgatc
     6481 tgttgagcag ccactgcatt cgtacttatt gcctgaatcg agaacatttg tgaattttct
     6541 gcctgattaa ctgagatggc atctttgatt tgttcagcag tcattttcac attgtcttct
     6601 gtttctaaac gatctttgac ttcattgagc actaaatcac tgacgatcat atctttatac
     6661 gtattgatca tttgcaggtt cgtattgaca tcattgacat ttgcagtgtc tgattgtggc
     6721 agtgtcacga tcaattgtgc ttgtgaactg tattttggtg tcaagatgaa aaatgttacg
     6781 attcctgaga tggccaagcc agcaaacatt gagatgatga ttattaccaa gtgtttgcgt
     6841 aatgtttcaa aaatttcttt caagctgatt gtctcttcca ttttttctcc actttcctat
     6901 ttattctcta gattcatttg atcttttaat tcttttttca cacgatcaag ttcttgatca
     6961 ctgacttttt gataggagat attatcttgc atgaagcctt ctccttgcag ctgatcttgc
     7021 ttaatcgtac caaatgcctc ccgataatct aaagcaattt ttttcatttc atcaaagttc
     7081 atgtctgttt tcatattcga ctctagagca ttcaaaacgg attgatagtt gctgacaccg
     7141 tttagactca atactttttt cgctacgcct tctacgatct ttctttggcg cccttgtcta
     7201 ccgtaatcac catttgggtc ttcataacgc atacgagaat aagataatgc ctgatccccg
     7261 ttcaatgtaa ttttaccgat tggaaagtca tagccatctt gtgagaaagt gaggtcatta
     7321 ttgacttcga taccaccaac tgcatcaacg agttccttta gccctttcat attgatagag
     7381 acatagtgat cgattgggat gtctacatat ttttgtacgg tatccatcgc catggcagct
     7441 ccgccgaatg cgtaagcgtg gttgatcttg tcttttgtgt cacgacctac gatgtcgaca
     7501 tacgtatcac gaggaatgct gacgatcgtt gtctgtttgt cttcaggact gactgtggca
     7561 accatcatcg tatctgatct tccttgatcg acccgaccta aatctccagt atcaatcccc
     7621 atcaatagaa cagagaaaga ttcttgtttg tccagatcaa ctggtttcgt tcgttctcct
     7681 tgggttcgtt ccactgattc gtaagttttc tcaatagaat gtgagagatc cagatataat
     7741 tttgctccta ctcctacaat tgctaatact aatacgataa gaactgctaa aataatcaga
     7801 aatatttttt tccctcttga ttttcttctt cgtcttgcca tagtttcctc cgtttgctaa
     7861 ccttgcttga atcaacgttt taactgtcaa tagcttatat tgctatagcc catttgctca
     7921 gcaacagttt gtgcttttgt tgcgtatgct gcgcttattt tttcattccg gatctgctct
     7981 atttgactga gcaccgtcaa atattgatcg taagtagcgg tggcagtcac ttgaccatcc
     8041 tttaacatcg tatcgaataa ctgatctagt tgcgtgactt gcgcgatctg aatatcagcg
     8101 aatcctagat attgcataac aaccgttttc caaggactat ctttaaaaaa gctcatattt
     8161 tcacgtacac gagcgacatc tgcacttgtc accttttctt tgatgatgac atcgtctaca
     8221 gcttgctgcc agtttgatac atttttagta aataaatcgt tgacagctgt ctggatcttc
     8281 agttttgatt ctgcactatc taactgtgtc acgattgctt tcttctcatc ttggattgca
     8341 acagcttctt taggcaattc tgattctttg atgtcgtaat catctgctga aagtttgaca
     8401 gaggatagct tatttgacaa acttgtgatt gtttcttctg ttatttcagg gtttagaaaa
     8461 attttttctt ttgtactata cagcttgtct acttcttttg ataaagaagt caaggtgtct
     8521 ttttgattgg tgatggcaga ttctgcataa ttgatccgtt gctgatgttg ccgctgatta
     8581 ttgatatggt atccaaacaa acagatcgca atagcacctg cacctaccgc cacagcagtc
     8641 aatgacttga cgaatggctt ttccatagat tcactcctaa gtattgatga tagataaatt
     8701 aagatataat tcacttacat aaaaatgaca gctttttatt taaaaaaaca taattaaact
     8761 aatatattca cagaaatatt tgtaaacaag ttttcccta
//
LOCUS       DAZULY010000125         8766 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000125 DAZULY010000000
VERSION     DAZULY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.126"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..295
                     /locus_tag="ACG81A_001847"
     CDS             <1..295
                     /locus_tag="ACG81A_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015205.1"
                     /translation="YTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKPVR
                     TRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(392..826)
                     /locus_tag="ACG81A_001848"
     CDS             complement(392..826)
                     /locus_tag="ACG81A_001848"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5015206.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1058..1768)
                     /locus_tag="ACG81A_001849"
     CDS             complement(1058..1768)
                     /locus_tag="ACG81A_001849"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS5015207.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1758..2618)
                     /locus_tag="ACG81A_001850"
     CDS             complement(1758..2618)
                     /locus_tag="ACG81A_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFS5015208.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2623..3261)
                     /locus_tag="ACG81A_001851"
     CDS             complement(2623..3261)
                     /locus_tag="ACG81A_001851"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFS5015209.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3276..3575)
                     /locus_tag="ACG81A_001852"
     CDS             complement(3276..3575)
                     /locus_tag="ACG81A_001852"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5015210.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3618..5102)
                     /locus_tag="ACG81A_001853"
     CDS             complement(3618..5102)
                     /locus_tag="ACG81A_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS5015211.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5122..5583)
                     /locus_tag="ACG81A_001854"
     CDS             complement(5122..5583)
                     /locus_tag="ACG81A_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5015212.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5601..6668)
                     /gene="ulaG"
                     /locus_tag="ACG81A_001855"
     CDS             complement(5601..6668)
                     /gene="ulaG"
                     /locus_tag="ACG81A_001855"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFS5015213.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6886..7656
                     /locus_tag="ACG81A_001856"
     CDS             6886..7656
                     /locus_tag="ACG81A_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5015214.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7929..8756)
                     /locus_tag="ACG81A_001857"
     CDS             complement(7929..8756)
                     /locus_tag="ACG81A_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5015215.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2649 a         1849 c         1381 g         2887 t
ORIGIN      
        1 ttatactgtt gaatttgaca gtgaactact taagcaattt ccacaagaag aacatttcat
       61 gtataaagaa gcaatttccc acaaagtgac agagtatttg gagcataatt tttttcgtat
      121 aaagccagtg cgtactcgtt cggcaaaagg aatatacgag atgaaagtca aaattaataa
      181 aaaaaattat cgcatagcat ttgctttaaa aaaaaatcat gtgttcattt tttatattag
      241 ttcgaatttg caaaaaattg tatttgataa agaggtgagt aaaaaaataa aatagctgag
      301 cattggagga tagattgagt gatactaagt ctatcctctt gtttttctaa agaactaagc
      361 tagaattttt tcttttgtac gttattatat gttatttgtc attaccaaaa agcacctttt
      421 ttcttctagt atctatgaca taagaaatac cacccatttc ctttacttga acaaatccta
      481 ctgattcata aaattttttt gttttctcag tatcatctgt caataattcg atttggactg
      541 gtccataact agtgatcttc tccaaagctt cattcaaaag cactttgcct attccttggc
      601 gctgatacct cggatgaatg aggatatctt gaataaatgc gatcgtcgca aaatcagtga
      661 tccaacgaac gattccgata atcttccctt ccccattacg ctttatcaga gattcagatt
      721 gttcaaatgc tctagctaac cttgcagtgt cttttgtgta attcgtccaa ccaacgctct
      781 tatataattc agaaatctca tcgatcataa gggaatcaat attcattttt ttcttccttt
      841 cttatacaat tctccctcta ataatacagt tctttgacta tttaatctat cacatataac
      901 tctcctgtaa agattctctc gattccttaa aattcctctt tccttttaga atgcatactt
      961 tttgataaat aaaaaggttg tgataaattt tcttcacaac ctctgtttct ctctatcaag
     1021 tgttatcttt taattttcta cccttaccct agcatctcta ttgcccgtag tatgcatact
     1081 ttccatgttt gcgaaaataa tgtttatcta gtaaatattg cgggatttca catatattgg
     1141 ggttgatcag ctcgttttct ttggccatca gacaaatttc atctaagatc aaactgtttt
     1201 ctactgcttt cataggtgaa tccccccatg tgaacggtcc atgtccatag acaagtactc
     1261 cagggacttc gtttggatct aattttcttt ccttaaacgt ctcgacaatc acattccctg
     1321 tatgaacttc gtaagcttct cttacttctt ctttcgttaa ttggcgtgta caaggaacct
     1381 taccataaaa agcatcagca tgagtcgtgc cataagctgg caaatcgcgt cctgcttgcg
     1441 cccacatgac ggagtgcgtg gaatgagtat gagtaatagc agttatatct tcaaaagttt
     1501 gatacaaaac aacatgtgtg gggagatctg aagatggttt taagctatct tcttctatta
     1561 tatttcccga caaatctgtt accaccattt gatctgcttg catgcattca tatctgacac
     1621 cgcttggctt aatgacgatg actcctagct cacggtccac ttcactcaca ttgccccaag
     1681 tcaatttgac taatccgtat cgcggcaatg ccagatttgc ttcaaggaca cgttgcttca
     1741 ttgctgcaat tttttcttca caagacatca tagccagcct ccttcaattt agaacttaag
     1801 tagtcttttg cctgctggat ttctcttcga aagtcttggc ttttttcact ccacatttca
     1861 atcagaaatg ttccatcata atctagacgt ttcaaatgtc ttaataatcc aagaaaatca
     1921 acacaaccgc taccaaaagg aacatcacga aactggcctg aatattcttt tgttacaggg
     1981 taagtatctt ttaagtggat agcagtgata taatcgatcc ctttctcaag ttctcgagcc
     2041 ggattatttt ccggccatgc ggacaaattg cctaggtctg gatatacttg tagaaaaggt
     2101 gaatgaatct gttctttgat ttctaaaaat tttgaaatgg agttcataaa tggatcatcc
     2161 atgatttcca cggaaagaat gatgccatat ttagaagctt cctttacgct ttcttttatt
     2221 ccttcgataa agtcttctct ggaattcatt gatttctctt cataataaac atcgtatcca
     2281 gctatttgga ttactttgac gcttagatta tgcgccaaat ggattgcttt tttcattagt
     2341 tgtttcgctg cttttttctt ttctggatca gcagaaccaa acggaaatcg ccgatgcgca
     2401 cttaaacaaa tcgaatggat acgtacaccg gagctgaaca tctcttctct cagctcagct
     2461 atcttttctt cagaccaatc taatcgtgct aaccgctcat ccgtctcatc aatcgacatc
     2521 tcgataaaat cgaagtccat ttcttttaca agggaaaaac gttctttcca tgaaatatct
     2581 ttgggtaacg ctttttcata aatcccaatt tgagccattt ccttaccccc agatacgaat
     2641 gatctcatct ttgaagtttt gtgcggcctt catcggatca tttgctgctg taataccacg
     2701 accagtgata aaggtataca catcgatccc ttcaaataag tgtaatgtgt gtggatttaa
     2761 tccaccagtt acagaaacat taaagcctag ttcaatcagt tttttgatct tcgataaatc
     2821 tttttcaccc caagtttcac cagataacag cgcatcacgg ctttgatggt agattacctg
     2881 ccggataccg atgttatgcc aatccatggc ttgttcaaaa gtccaattgc cgtagagttc
     2941 tacttgcaat tcacctattt ctttttgtgc tgcctccata gttggaatcg ttgcacaaca
     3001 aatgactgtc atccagtctg ctcctgcttt tgcaacatta ctcgccactg ttcctccagc
     3061 gtctgcacat ttcgtatcag cgactagttt tttatctggg aacatactgc gaatacagcg
     3121 aatcgctttt tgtccttcct gtaagcaaag aattgtgccc acttcaataa tatctactac
     3181 atcaccaact ttcataacat ctcctaatgc atgttctaag tcattgtgat ctaacgcaat
     3241 ttgcaaattc ggcctgctca tcttagttcc ttcctttact tgtttttttg gtaccacttt
     3301 gtttcagcaa atttttcact gatttctttg actgacatca catttttgat accgataact
     3361 gtaactcctt ttttctctgc ctgagtaaac atattcaaaa aattgactgg cgtaaaaact
     3421 acatcgtatt ggccagctga actttttccc tctgaaatgg cacaatgatg gattttggta
     3481 attggtacgt ttagttcttt cattgctttt tccacacttc tcatcatcat caagcttgtt
     3541 cctgatccat ttgcacacga tactaaaatt ctcatcttaa tccctccatt atgagtgttg
     3601 tctaatcaga ttttctttta tactctctgc ttttctgcat atgtgtcata gtcttctaca
     3661 atcaaaaaat aaccatcagg atttttccga tattgtaact gcggaatagc caataaacca
     3721 atgacaacta cggcaattcc agcataacct aggactttca tgattactgt aaacaatggc
     3781 caagctgttg cccagtcgaa catcccgata taaccaccgt atgttgacat cccaatccat
     3841 gtagcgaata atgctgaacc gcctacttgt atgattcctg aaagaaacgg gaacaggcaa
     3901 gcggctttga atccacctcg gttattggca taaactgcta taacggcatt atcaaagaac
     3961 aacggaatga aaccggctat gacgatcgtt ggtgatttca ataaaatcag taaaccaatc
     4021 atcaaaaact gtcccaaagc accaaacaag aacccaatcg ttaccgcatt tggcgaacca
     4081 aagccaaaag tagcagctac gtcaattcct ggaacagctc ctggtaaaat cgtgtttgag
     4141 attccttgga aagattgagt tagttcatcg acgaatgtcc gaacacctaa ttgcaagatc
     4201 gccagatata ccgcaaaatt taaagaagtc gtcataatgt agaataagaa actctgccct
     4261 tcttgcatga attgcgcttg aatcaaataa tctttgccta gtacaatcag aatgatccca
     4321 aagaaaaata gcatgagtaa cgaagtagca accatattct cattgaaaat cgacaagaat
     4381 cctggtaatt tgatatcttc aatttttttg tcttgcttga ttgatctatt tttcttttca
     4441 tcccgctttt tcatccactc agctaatttt gcaaatataa agattccgaa catttgctga
     4501 tgtgccactg caaatccggc accttctgtc aattcctgcg tgattcctac tgttaagtta
     4561 gaagctactg tccaataaca acctaaaatc agtcccataa aaatcaatgc ttgtatccta
     4621 cctagatcag gaaagcaaaa cagtaaaatc caaaaagcgg tagctgcttg ttgaacctga
     4681 acgtttccgg tagtgaaaac ggaacgcagt ttcgtatatt tctgaaaacg gactaataag
     4741 atatttaaaa caaatgcaat cagtaataaa atcatcacat ctccaaaggt acggccaaat
     4801 gtctccatta atccagcatc cacagcattt tgtccaaaat aaggatctgt aaccatggca
     4861 tccaaattga accgttcctt tagtccaacc aagattggcc gaaaattatt gaccaaacca
     4921 ccagagccaa cagtcaaaat aaagtaaccg accgttgcct ttaagaatcc agcgatgcat
     4981 tcatgaagcg ggcgtttcag caaaatatag cctaataaca cgataaaacc aatcaggtat
     5041 gctggctgcg tcaagatatt tgttgcaaaa tacgtccaaa tgcttaacaa aaattccccc
     5101 atcttttttc tcctctatca attaattata ttctgccatc acaacttggt aatcatcgat
     5161 ggagttcgta gagatgagac gctcaaccaa tccatctgtc attaataatt cggataattt
     5221 ttggatattt tctagatgtt cttctggatc ttttgctgcg agtgtaaaaa acaaactagc
     5281 cgttttctcc tcattctccc cttcactttc aaaacggatt ggctgtttca acttggtaaa
     5341 tgaaatagct gttccaaaaa caccatcact tttttctgaa gaatgcggca ttgctacttg
     5401 aggtgcgatc acaatataag gaccgtattt ttctacacaa tggatgatct cctccacata
     5461 cgtttcatca ataatttcct tatcgatcaa tacaagacag ctttcggcga tagcttcacg
     5521 ccaatccgtg atttctttat ctgggtaatg aatcaattga tggtcaaaaa agtatttcag
     5581 caagaaactc cccttctttc ttataaaaac gacggaaacg gtgtatcatt ttcaatcgag
     5641 aagacatctt caaatcctcg ataaaaatta aactcaagat catctttgtt tgtcggatag
     5701 atgaatttcc cgcctacttg ccagatataa ggtttaaatc catacttcag gcgatccttt
     5761 ttgaatttcc aaatttcagt gatctctttt ggatcagcca taaaattaga ccaaatatcg
     5821 tagtggacag gaatcactac ttttgcattc aatgactccg ccatgcgcag catatcaaca
     5881 gaagtaactt tgtctgttat ccctcgtggg ttttcaccat aggcccctaa acagacatcg
     5941 attttgtgtt cattaccatg tttggcaaat aaattggagt aatgtgaatc tcctgcatga
     6001 taaagattcc ctcctgatgt ttcgaataaa tagtttactg caatttgatc catctcctga
     6061 ggcatttttc cttttaatac ctcttctgga tcttcacaag tgaccaacgc tgtccgatca
     6121 aacgcttcta aagctactac attgatgtct ttgattttga ctgcatcacc tggctttact
     6181 gtaatcgttt tttcttcagg aattccccat ttttgccacg tgcgaaccac ttcttttggc
     6241 ccaatgaatg ttgcttcagg acaattttga tgaactgctg cggctgtatg gatatctaga
     6301 tgatcggaat ggatatgtgt aacgattaaa gcgtctacat tttttatcgc aaaaggatcg
     6361 attacgaaag gctgagtacg taaattaggc tgcatatttt gaacgccact catacgcatc
     6421 atctgatggc cttttttcat catgccattc ccatgactac gttttcccgt cccacaccat
     6481 agatcacata aaatattcgt tccttcatga cttttcagcc aaattcccgt acagcccagc
     6541 caccacatag caaaagttcc aggatccact acttctgttt cgatttcttc attcaagtat
     6601 gtgccccact caggaaatgt ttctactaac cacttttctt ttgttacttc attgacagtt
     6661 ttactcattt gcttgctccc cccatatcgt tgttccaact tacaagtaaa tattaatgtg
     6721 tatcagcatc catgaaaacc cttttatatg accgtttgtt ttttatatga ttttctttca
     6781 gtaatcaatg attgtttcag tataataaag acagatatga atgtttaaat gaacaactag
     6841 tcaaataaat ccatggatca agatgtccct caggaggcaa aagtcatgaa aaactcactt
     6901 gtcaatattg aaaaacgtca aaaagaaatt ttggaactgc ttcagcagac tgaacaacta
     6961 tcaactttcg aattagctga atcccttgat gtatctatta gtacgatacg aagagattta
     7021 aatttattag aagaaaaaaa cgagatcata cgcaattacg gttattgttc ttataattac
     7081 aaaaaccaga ctgattttga tcaatctggt cctgaaagga ttaaacaagc gattgccaga
     7141 gaagctagta aatacattaa cgattatgat acgctgttta tcaattctag ctcgactgcg
     7201 ttgaaaacac tggattttct tcaatctaac catttgacag tcattacaaa caacgtgaaa
     7261 atcaattact ctccgcacaa agataattgt agttatattt taactggtgg agagatgcgc
     7321 tttccaaaag aagcactagt gggcgacatc gcattaaata cgatttcttc agtcaatgca
     7381 gacatttgta tcattggctg tagcggagtc gatgtagtac atggggtcac aacaaagata
     7441 ttaaatgaat caacgatcaa cgaaatgatg gtcaacaaga caatcaaatg taagatttta
     7501 gtcgctgatc atcgaaaaat cggactgaca tcaaagttca agattgctga tatttcagtc
     7561 tttgattatt tgatcactga taaattttgt tctgaaaaag tattagagga gatcagaaat
     7621 acggggataa aaaccattca agtagattca ttttaagaac caggacgtaa atatgtaaat
     7681 aggattatca aaaaaatgaa taacaaattt atcgataaat gaaaaacacg ctacgctcaa
     7741 attgataaag gaatcccaag atgtatccat ccaatcgata ccatcataat ctattgcttc
     7801 ttttataacc gttatatgtt gcctaaatag gaatcaaaca atatcgaaaa aaggaggtat
     7861 gacaaaagtc acacctcctt ttttcgaata aacggtattc aaatcagaac actgatttca
     7921 caaccacttt atttcaggtc gttttcaatc acctttaccg cgtaatcatt tccgttgaaa
     7981 cgcgctattt tttttgcgtc catatatctt tctaaataat catcttctcc taattgctta
     8041 tggcaagaag caatttcata aagcaagtat tccaatgcat aagtcgattt atgtttctta
     8101 ttatactcaa tacccttctc acataattgg attgctttat cataatcctc gattttacgg
     8161 taataggtgg cgatatcata atatacaatg cagaccatca gattatcaaa atcagtctca
     8221 ctcaatattt taagtgtacg ctccacataa actttcgcaa aatctaattc tccttttgac
     8281 aagtagatca ttgccacgtt tgcagctgct tgtaagtgat ggatatctgt ttcggtcgtt
     8341 tgttccaaca cttgattaaa ataaaataat gctgcatctg gttttttcgc ataaagatga
     8401 tcaagcagtc cttgataaaa taaaatttga tttaaaagat cagaattact tactttttcc
     8461 tgatcgatcg tttgtaaaat tttataagat tcttggtatt ttgatacaga attcaatgat
     8521 tgaatctcat ttattttttt cctgattttg tcttcttctg tttctactaa tatatcggag
     8581 atctcgacat tcagcttttt gcaaagcgca cttaagatat tgatgcctgg cgacatattc
     8641 ttgttttcga tattgctgac agttgcttgt tcacaaatgc cttctgctaa ttgtacttgt
     8701 gttaacccct ttttctttcg gtattctttt agtaatgctc catctacttt aatcaaaaca
     8761 atttcc
//
LOCUS       DAZULY010000126         8581 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000126 DAZULY010000000
VERSION     DAZULY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.127"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(165..2546)
                     /locus_tag="ACG81A_001858"
     CDS             complement(165..2546)
                     /locus_tag="ACG81A_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFS5015216.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2536..3183)
                     /gene="recU"
                     /locus_tag="ACG81A_001859"
     CDS             complement(2536..3183)
                     /gene="recU"
                     /locus_tag="ACG81A_001859"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFS5015217.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3247..3786
                     /locus_tag="ACG81A_001860"
     CDS             3247..3786
                     /locus_tag="ACG81A_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFS5015218.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3876..4295
                     /gene="gpsB"
                     /locus_tag="ACG81A_001861"
     CDS             3876..4295
                     /gene="gpsB"
                     /locus_tag="ACG81A_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFS5015219.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4317..4684
                     /gene="rnpB"
                     /locus_tag="ACG81A_001862"
     ncRNA           4317..4684
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG81A_001862"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4893..6059
                     /locus_tag="ACG81A_001863"
     CDS             4893..6059
                     /locus_tag="ACG81A_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFS5015220.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6105..7601
                     /locus_tag="ACG81A_001864"
     CDS             6105..7601
                     /locus_tag="ACG81A_001864"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFS5015221.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7682..8365
                     /locus_tag="ACG81A_001865"
     CDS             7682..8365
                     /locus_tag="ACG81A_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5015222.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8374..>8581
                     /locus_tag="ACG81A_001866"
     CDS             8374..>8581
                     /locus_tag="ACG81A_001866"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015223.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRLFGRIG"
BASE COUNT         2684 a         1566 c         1829 g         2502 t
ORIGIN      
        1 atggattttc taatttttaa gctaaatgtt agatcacaca tgtatatgct ggatagtctt
       61 ataaaaaatc atgtaataca gcttggtaca tgatttttat ttgtagctgt tttttacata
      121 gacacaaaaa agaaaccacg cttgtagacg tggtttcttt tttactatct gtttccagaa
      181 gctgaggtgc tgctggatgg tgtatggtca tttgcagcat ccgctccgct agttgctggc
      241 tcactggaag aagcaggagg agcagatgaa gacggtgtcg agctttcttc ggatgaagaa
      301 gaaggaaccg ttgttgaaga cgctggctcg cttgtttctg ctgtcgtgct ttcttttgag
      361 gactcagcag ttgattcgct ttgactagta gagctagagc tttgagtcgt tgtctctgtt
      421 gttgagcttc caggagtctg gacatagctg gaagatggaa tagttgttga tggtgtaatt
      481 gcattcgatc tagctgtata ttgatctttg tagtacagtt caccgcccac acgaattaat
      541 ccgcttggca tctcccagtc tgtatttgtc acgttagctg aaacatactg cataagctcg
      601 cgataaacat cagaggctac gtgtgacgat tctgaagtga caggtgtcat ttttttattg
      661 taacctgtcc acacggaaat cgaataattt ggcgtatagc ccgcaaatag gatatctgga
      721 tacaccccag aagaaatacc aagttttgca tactcgtcat ctgtgtaatt ggatgttccg
      781 gtcttaccgg cttgatataa cccagcgatt tgcgcattcg taccagttcc ttcagtgatc
      841 gtatctttta aaatgtctgt gatcatataa gctgtttctg gagacatggc tgtttttcca
      901 tctggctcat aagtttcttc tgttccgtct tggaagacga ttttattaac atattgaggt
      961 ttgtaataag tccctccatt ggcgaaagcc gcataggctg ctgccatttt taaggaagaa
     1021 gctccgtatt ttgtcccatc ttgttcttcc gtattactag agatcgcatt cgattgatgg
     1081 atcgtactgt attcgatccc aagatttttt aagaaagaag ccactttatc tgaaccgact
     1141 tcattgaata atttgactgc tggaacatta cgggataagt aaagtgcttg gcgtaaagta
     1201 atggtgccca tatattgatt atcccagttt cctacaggag tcgaagtccc ttcatagtta
     1261 tacggtgcgt cagtaatagt ttttccagta gaatatttca aatattcaaa agctggtccg
     1321 taatctgtga caggtttcat cgtagaacca aaatcacggg aagtattcac tgctaagttg
     1381 ttgcctaaag ttacgtcttc tgcgatgtga cgtccgccaa tctgggcttt tactttcccg
     1441 gtattggtgt cgataagggt ggaggcaact tgcatctcgt cgtctgggta agagacatac
     1501 tgatccgtgt tgacgatatc atatagtttc ttttgggcgt ccagatccaa attcgtataa
     1561 atctctaaac cgtctgtgta gacattctta tctgtttttt cttgtacttc attgatgact
     1621 tctttgacat aattatcgac gatcttagta ttgtcatcgg attgagttag ttcttgcaga
     1681 ccatccgtta ccggtacatt aacagcttga tcatattctg tttgagagat tttttcgttt
     1741 tgaagcatcg tatacaaaac agtatctcgt cttttcttcg cctgatctgg ataaacataa
     1801 ggatcatagg cggaaggggc ttgaggcatc ccagcaagca atgctgtttg cggtaaagaa
     1861 agttctgata atttcttacc aaagtacatc tcagaagctg tctccatccc gtacaagccg
     1921 tttgacatat acactttatt tacgtagtag gtcaagatct cttgtttcga ttttttttgt
     1981 tccaatctta cagccatcca cgcttcttgg gccttgcgtt tcagagtctg atcctcggca
     2041 ctggtggaga agaaggaaag tttgatcaat tgctgtgtca aagtacttcc gccttgcagt
     2101 ccgccagagg taaagttaga gagggcagaa ccgatgatcc gaatagggtc aaccccgatg
     2161 tgtttataaa aacggcgatc ttctaccgac acgatcgcat cttctaatgt tttcggtaat
     2221 tcattcgcag aaattttttc acgcttttct gcgcctaaat cttcgaatag ttcgccatct
     2281 tgtgtatata gtttggaaga tacggtagcg tctaattttt tatccgtcaa ttcgggcgca
     2341 tcttttgcgt aataccagaa aagtcctaca cctgctaaaa agaggataca gaaaaatgag
     2401 agtattccta aaaggatctt gataaataat ccttttttat gtgttccaga ggactttcca
     2461 gaacctttgc cagaattctt cttcacactc tttttaggag taggctgttt ctgtcgtcta
     2521 gagcttctag tttgttcatt tgccattctt gctttctcct tcaatagata atgttgataa
     2581 atagaagtca accgcgtcca aataaggaat ccgcggagaa ataccagttt tgatctctat
     2641 tcctagttct tcgatcaaag ataaaggtag tgatttgcgt cctttttcct tttgggcgtc
     2701 ccagtaagca atcaaatgaa cactattcaa ataaaaacag cgcttcaaag acgaaaacca
     2761 taacaagaca aagcagaccc cattttgttc gaggcatgct ttcatatgtt cgatttgatg
     2821 ggcatggaag tttttaaagg gaaaagatgt tttgttcttg gtttcttttg cttcaaagtc
     2881 caagtaaaag cctcgataga cgccattata gtctgtagtt gaggcttggg taaagtaggc
     2941 ttccttgatc acagcagcac ttctcttagg atagtctact ttcacgattt ggataggggt
     3001 aggcttttta tggataaccg ccaacccgcg ggaaagataa tatttattac tttcattgat
     3061 tgcttcttca aagcgcatcc cccgattgcc gaattcagtg atctgatttc ttttttcttg
     3121 aattttttcg acgggagagg cattcttata ataaggttgg ccatttggat agcgcaatac
     3181 catctttaat cacactccta ggccacatta tagcaaaaaa gcggatagaa agaaaaggaa
     3241 tctgagatgg ataagtcaaa agtaatctat gtgtctgggt atcgaagttt cgaactcggt
     3301 gtttttaaag aaaatgatcc taaaatccaa gttgttaaaa atgttttaaa aaaagaactc
     3361 catcagttag cagaagaagg gctggaatgg gtacttgtca gtgggaattt gggcgtcgaa
     3421 ctatgggctg tgcaaatagt agcggaattg aaagaagaat atccggaact atctcttggt
     3481 attttgtatc ctttttctga ttttggtaat aactggaatg aagggaacca agaaaaaaaa
     3541 caattagcag aacaattagc agattatgtc gaagctgtta gccatcaacc ctaccaatca
     3601 cctagtcagc taaagaatca cactcgtttt atacttgagc atacagtggg ctgtctgctg
     3661 atctatgatg aagaattccc tggaaaaacg aagtattttt ttgaagatgc acagaattac
     3721 caagcagatc atccctacga aattcggctg atcagtatgg acgatcttca aaattttagt
     3781 gaataatcga tattgtgttt gatttatcaa cttttttcct atacaatgaa aaaggtagaa
     3841 atgttattac gtaaatgaaa actagaggtg taaatatggc gaatttggtt tatagtccta
     3901 aagacatttt acaacaagaa ttcaaaacaa aaatgcgcgg ctatgatccc gttgaagttg
     3961 atgaattctt agacaatatc atcaaagatt atgaagctta cagcaaagaa ctccttgctt
     4021 tacaagaaga aaacgatcga ttgagtgcaa aagtagctca attatctaaa actcaaggag
     4081 cagctcaaac acgcgtgcaa caaacagagg caccaaagag cgcagcagtg accaattttg
     4141 atatcttaaa acgcttgtca aatcttgaac gtgaagtatt tggtaaaaag cttgatcaac
     4201 aagcaagtgc agtcaaacca gcacaaccta atccaaataa ctacacaaat gcagacacaa
     4261 gcttagatga taatgaaaaa acacgtcaat tttaattttt gattaatgaa tttgcggttt
     4321 tcgggtaatc gcggtctgac ttgatcaggc tgaggaaagt ccatgctcgc acaggctgtg
     4381 atgcctgtag tgttcgtgct tagcgaaacc ataagctaag gtactctttg gagtaacggc
     4441 aggaaaacaa gctaaggctt cggctatgct taagtatcct tgaaagtgcc acagtgacga
     4501 agcggctggg gaaacccatc cggtggaacg cggtaaaccc ctcgagcgag caacccaaac
     4561 aatggtaggg gcgctttttg tctggaagaa gaacaggaca aaaaggcagt ataactgcag
     4621 atagatggtt accgctttgt tttttctccc tggaaaagca aagtacagaa catggcttat
     4681 agaaaaccgc aaacatcagg gaattaggtc gtgacaaaag taatgcgtta taagaaacaa
     4741 gaaaaacatt tgaaagctat ttttcaatac ttcagatgaa tcgttcttat tacaaaacaa
     4801 acttttgtat caccggttat tcggaatttg agtaaaagtg tggggtagga ctgatgtccc
     4861 acctcctttt tttataaata aggagattgt gtatggaaaa aaacaggaag ttcaacctcg
     4921 tcgcaactgc ggcaagcggg ctagaagcac tggttggtaa agaattaaga gatcttggaa
     4981 tcgaatgcca agttgagaac ggacgtgcac gttttgaagg aacaatggaa acgatcgcta
     5041 cagctaattt atggttacgt acagcagatc gagtaaaaat cgtggttggg gaattcgatg
     5101 cgtatacgtt tgatgaattg tttgaaaacg taaaagcatt gccttgggaa gattttcttc
     5161 cactagatgc agcttttcct gtagctggaa aatcgatcaa gtcgaaacta tatagcacac
     5221 ctgattgcca agcgatcaca aaaaaagcaa tcgttgaacg tctccgtacc tattatcacc
     5281 gtccagcttc tgtaccactg acggaaactg gcgcacattt tcagttggaa gtagctttgc
     5341 tgaaagacca tgtgatggta actctggata cgacaggacc aagtttattc aaacgtggtt
     5401 atcgcttaga aaaaggtgga gcaccattga aagaaaacat ggcagcagca cttattatgt
     5461 tgacaaattg gcgaaaagac cgtccatttt atgatcctgt atgtggttca ggtacgcttt
     5521 gtattgaagc agccttaata ggccataata tcgcaccagg attcaatcgt gagtttgctt
     5581 gcgaatcttg ggattggttc agtcaagaaa tcatggaaaa tgttcgatca gcagccgaag
     5641 aaaaagcaga ttatgatatt gaattagata ttactggttc agatatcaat ggacggatga
     5701 ttgaaattgc caaagccaat gcagaagaaa tcggattagg ctcttcgatt acctttaagc
     5761 aacaagcagt caaagacttc aaaacggata aagaatacgg ggtcatcgtg gctaatccgc
     5821 cttacggaga acgtctagga gaagaagaaa ctgttcggaa attatataaa gaaatgggcg
     5881 atgtattccg tccattaaaa acatggagta aatatatctt gactagcgac ttagcgtttg
     5941 aggaatttta cggacaaaaa gcaaccaaaa aaagaaaatt gtacaatggt gcattgcgta
     6001 cggatctatt ccagtattgg ggaactcgtc cgcctagaaa aaaagagaaa gacgaataat
     6061 cttactaatt gattcattca attggatgtt tggaggaata gggaatgaaa gaaaaagagt
     6121 ttttacaaga agtaaaagaa atcaatcttc tacaacaagc tttaggaatc ctcgattggg
     6181 atacacaaac aggtatgcct gaaaaagcta gtgcataccg aagtgaagta gacagctatc
     6241 tttacagcat ttatttttca aaaaaaatcg gtccaaaaat caaagaagcc attcgctatt
     6301 tttctgaaca tccggaagaa ttgtcggaag ttggaactgc cgtttttgcc aaagtaaaag
     6361 aagagtatga tttggaacat cgcgtaccgg aagatcttat gagaaaatat acagctgccg
     6421 tttcttcagc acacggacaa tggcaaaaag ctagagaaac acaacaattt tctgattttc
     6481 aacacgcatt gaatcaaaat attgaattaa caaaacagtt gataccttac tggagaaaag
     6541 atgaaaagac gaattatgat gttttgctga atcaatacga acccgggatg actgtcgaaa
     6601 tccttgataa ggtatttgat cagcttaaag aaggaattct ttcgatccgc cgtacgctcc
     6661 aagaaaaagg gactgaacct gaaacagact ttttaaatcg gagaatgacg aaaaaacaac
     6721 agaagagatt tgtagtgaaa gtaatcgaac aacttggtta tgattttagt cgtggtcgtt
     6781 tagatgacac tgtccaccca tttatgactg gaattaatcc gaatgatgca cgaatcacga
     6841 cacgatggaa cgaaacagat ttcaaaatgg ctgtttttgg agtgatccac gaggctggtc
     6901 atggtatgta tgaacaagac atcgatgata aatatgcgta tacaccggtt tatgaaggta
     6961 catcaatggg tatccatgaa tcacaatcgt tgtttaacga gatcattatt ggaagtagtc
     7021 gcagcttctg gaaaaagcaa tatcctttct ttcaagaatg tgcagaagga acattcgatg
     7081 atattccttt tgacaaattt tatcgttcat tgaaccatac aaaatcaagt ttggttcgta
     7141 tcgaggcaga cagtttgact taccctctcc atatcatcat tcgttatgaa atcgaaaaaa
     7201 tgattttcaa tgaaggggta gatgttgctg aactacctga aatatggaat caaaaatatg
     7261 aagagtatct gggaatacga ccaacaaatg atgtggaagg catcctacaa gacgttcatt
     7321 ggtcaggtgc aagctttggt tattttccct cttatgcatt agggtacatg tatgcggcac
     7381 aattacttca ttctttgcag caagaaatag atgtggaaaa tgttctagcg tcagacgatt
     7441 attctccaat caaaaactgg atgtcagagc atatccatca atatggtgca tctagaaaac
     7501 caaatcaatt aatcatggat gcaacacatg aaccgcttaa tccgcaatac ctgatcgact
     7561 atatgaaaga cctgtatttt aatgtatatc aggtaaaata aaatacaatt tacaaagata
     7621 gctgaaattc atctaatcta tagatgtttt cagctattta cttaagaaag aagtggaacg
     7681 aatggataaa ttacaagtga tcgctgatta tagtatggac caattagcgc atgatcaaac
     7741 aggacatggg agcgatcata ccaaaagagt tgtcaagtta gctgaaaaaa ttttggagac
     7801 ggaaccgcag gcagaccgct ttgtgacgct tgcagcagct tacctacatg atacgattga
     7861 tgataaagtc gttaaaaatg agaacgaggc aaagcaacag cttcgtgtat tccttcgctc
     7921 tttgccaata gtagaggaac agatctcgat gattttttcc attatcgaaa atatgtcctt
     7981 ttctaaaaat ctctctgaag cggccgaact atctctggaa ggtaagatcg ttcaagacgc
     8041 tgatcgtatc gaagcattag gagcaatcgg cattttgcgt actgcttatt tcggcggtgg
     8101 acatgggcac ccgatttttg atcctgaact gtatccgcaa acattcaagg acaaagaaaa
     8161 ttaccgaaaa ggcacgacag tgatcaatca tttttatgaa aaactgttct tgctgtctga
     8221 taaaatgaat actgattacg gatatgaaga agccaaacga agagaaacct tcatgcgtga
     8281 attcttagaa gaattttttc tggaatggtc tattgacgat gaaaatttta ctccagacaa
     8341 ttggatcaaa aataacaaaa aatgatggag gttgtgacag aagtggtcag ctccaagata
     8401 tttcggatca ttttccgtaa aagttacttc tggaacactg tttaccaaga ggtcgtgagc
     8461 ctgccgttca gctccgagtc acaaggaaca atttcaaaaa atagcttctc gtgtttgatg
     8521 aatattgtaa cttgtgattc ggaattggca ggagaacacc gtttattcgg aagaatagga
     8581 c
//
LOCUS       DAZULY010000127         8570 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000127 DAZULY010000000
VERSION     DAZULY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.128"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            112..1239
                     /locus_tag="ACG81A_001867"
                     /pseudo
     CDS             112..1239
                     /locus_tag="ACG81A_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
     gene            1337..1525
                     /locus_tag="ACG81A_001868"
     CDS             1337..1525
                     /locus_tag="ACG81A_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFS5015224.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1583..1936)
                     /locus_tag="ACG81A_001869"
     CDS             complement(1583..1936)
                     /locus_tag="ACG81A_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5015225.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2467..2889
                     /locus_tag="ACG81A_001870"
     CDS             2467..2889
                     /locus_tag="ACG81A_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5015226.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2886..3116
                     /locus_tag="ACG81A_001871"
     CDS             2886..3116
                     /locus_tag="ACG81A_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5015227.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3614..3814
                     /locus_tag="ACG81A_001872"
     CDS             3614..3814
                     /locus_tag="ACG81A_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HFS5015228.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3841..5034
                     /locus_tag="ACG81A_001873"
     CDS             3841..5034
                     /locus_tag="ACG81A_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5015229.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(5102..6667)
                     /gene="guaA"
                     /locus_tag="ACG81A_001874"
     CDS             complement(5102..6667)
                     /gene="guaA"
                     /locus_tag="ACG81A_001874"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS5015230.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(6726..7649)
                     /gene="coaA"
                     /locus_tag="ACG81A_001875"
     CDS             complement(6726..7649)
                     /gene="coaA"
                     /locus_tag="ACG81A_001875"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFS5015231.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7845..8450
                     /locus_tag="ACG81A_001876"
     CDS             7845..8450
                     /locus_tag="ACG81A_001876"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5015232.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
BASE COUNT         2796 a         1538 c         1621 g         2615 t
ORIGIN      
        1 ttgcatttta taaatcattt tgtatgtaat ttggtatgta agttaaaatg aaaaaagtag
       61 tccattttga agtacatttt gtcattttgt actccaaaac tgaactactt acttgcatat
      121 atacgccttt atagtggagt actacattta cgagattcgg ttagagtatc agaaaaagaa
      181 aaaataaaag ttacagaaat gtatacttca ataaatggtg aattatgtaa gattgataga
      241 gcttattctg gagaaattgt tattttgcaa aatgagtttt tgaagttaaa tagtgttctt
      301 ggagatacaa aactattgcc acagagaaaa aagattgaaa atccgcaccc tctactacaa
      361 acaactgttg aaccgagtaa acctgaacag agagaaatgt tgcttgatgc ccttttggaa
      421 atctcagata gtgatccgct tctacgatat tacgtggatt ctacgacaca tgaaattata
      481 ctttctttct tagggaaagt acaaatggaa gtgattagtg cactgttgca agaaaagtat
      541 catgtggaga tagaactaaa agagcctaca gtcatttata tggagagacc gttaaaaaat
      601 gcagaatata ccattcacat cgaagtgccg ccaaatcctt tctgggcttc cattggttta
      661 tctgtatcac cgcttccgtt gggaagtgga atgcagtatg agagctcggt ttctcttgga
      721 tacttaaatc aatcatttca aaatgcagtt atggaaggga tacgctatgg ttgcgaacaa
      781 ggattatatg gttggaatgt gacggattgt aaaatctgtt ttaagtacgg tttatactat
      841 agccctgtta gtactccagc agattttcgg atgcttactc ctattgtact ggagcaagcc
      901 tttagaaaag ctggaacaga attgttagag ccatatctta gttttaaagt ttatgcacca
      961 caggaatatc tttcacgggc atataacgat gctcccaaat attgtgcaaa tatcgtaaat
     1021 actcaactga aaaataatga ggtcattatt attggagaaa ttcctgctcg atgtattcaa
     1081 gattatcgca atgatttaac tttttttaca aatgggctta gtgtttgttt agcagagcta
     1141 aaaggatatc aggttaccac tggcgaacct gtttgccaga cccgtcgtct aaatagtcgg
     1201 atagataaag taagatatat gttcaataaa ataacttagt gcgttttatg ttgttatata
     1261 aatatggttt cttattaaat aagatgaaat attctttaat atagatttga attaaagtgg
     1321 aaaggaggag attgttatta taaactacaa gtggatattg tgtcctattt gtggaaataa
     1381 aacaagacta cgaatacgag tggatactat acttaaaaat ttccctttat actgccccaa
     1441 atgtaagaac gaaactttaa ttaatgttca aaaaatgaat ataataacaa tcaaagagcc
     1501 agacgccaag acgcagagcc gataatttga gaaatgaaac tctcatctta tcggctcttt
     1561 ttgtttatct gaattttact gactagcctt caatatttct tgcttcattg ataccttttg
     1621 ctagtgcttc cattaaggat agttctttgt ctgtaaagct atccatgtat ttttctacct
     1681 gtaaccttcg agtgctttta atcaaattat ctgtaggtaa gaaaaattca tcaatagata
     1741 catggagtaa tgatacaaga tcatacagca cttgtgtact tgggtgttgt cctttgtttt
     1801 ctatatttgt taaataacgt gggtcaattt caatcattgt tcccacttgt tcacgagtga
     1861 gaccccgttt cattcgagcc tctttaatcg ctaaaccaac ggctctaaaa tcgtatttat
     1921 cttctttttt acgcatacta aatcacctct atacatttta ctgttcctat ataaccataa
     1981 acaggtatag aaaaacatgt tatatagttt atgagttcct atagaacact gtggaattta
     2041 ttaatgcgat gaatccaatt tcaaatagaa aggtcgctga ttttatttac ctatacaact
     2101 ttataaaatt tagtcatact cctagaaagt agataaaatt ggcgacttaa tggagggaaa
     2161 agtcgccatg agaaagataa tctgtcatct agttctctta attaagttct tatcaaaaaa
     2221 acttatccac ctgtactgga tagatatgac catcaatgtg gttttgcaga ttacatagat
     2281 agaaaaatca aagcatatga acagagaaaa caatctgttt atgtgctttt ttggtcactt
     2341 aataaatatc ttttaggatt tctcttcatt tatgcaacaa atcccccgtt cgtgtcgggg
     2401 tttagtatga agggagataa agaagcacat gccactccct ttcacgacag aaagggggtg
     2461 aaaagcatga aaccatcttc ttttcaagaa accatagaaa atcagtttga ctatatctgc
     2521 aaacaagtta ttgaagatga acgaaaagat tacttcaaac atctagacag actatcagca
     2581 aaagaagtct cgttttctga tatagataac taccttgtca actctttttc tactgttgat
     2641 acgtacgtca ctgatttcca actatttaaa ttatttggat taagaatcaa tgttgaaagt
     2701 gatttattaa gtgaagccct gcgtcatctt tcagagaaaa aacggaacat tattttactt
     2761 cattacttta tggatatgag tgatgtggag attgcggagt tactaaattt gaatcgttcg
     2821 actgtttatc gtcatcgaat aagtggacta gccatgatta aagaattcat gaaggagtgt
     2881 aaagaatgaa aacagcttat ccaagggttc ccttccccct cattgtaaag gcgacagatg
     2941 gcgacgtgga agcaattaat cagattgtta aacattatcg agggtatacc tcaaaacgtt
     3001 cattacgtcg catgacagac gaatatggaa attctcatat ggttattgat gaaaccctac
     3061 gtggcagaat ggaaacgaaa ctcatctcta agattttatc gttcgaaatt aaatagtccc
     3121 attttttcgt ggaagcgtgt cgaatcgtat ccacgcttcc cgatttggga ggtttgttat
     3181 tccatcaaag catattgtgt gttcagtagg ttttgatagg ctaacaagcc aaacgttctt
     3241 tgacaaccaa ataaaggcaa tcgaatacgt ttcggtaaga aaagagctga tgaactaaca
     3301 cgccatgacc tacaaaatta gtgagcgatt catgttagta attcgaaagt aatctttagc
     3361 aggattgctg ccatgacttt cttatcgtga taatgatact cccgtatagc catagtccga
     3421 gcgttagaag cgtcacaggc aatgggtacg gctacatgag aaccatgcag aagtgaaatt
     3481 cctgtgagct ttgctagagc tgttcgattg ctaagatatt cttctcttgt cagtgtatta
     3541 ttataaaact atttgatata attataggtg aatattggag ttttcctagc ctataattgg
     3601 gaaaggagta ataatgataa cggtcacaaa aaaggattta gttgagctag gatatggaac
     3661 ttctttttca gcagacatca taaaaaaagc aaaagaattg atgattacaa aagggcatat
     3721 ctactatcaa tctcgaaagc tagatagagt ccctcgtgaa gctgttgaag aaattctagg
     3781 aattcggttt tcaaatgaaa acaggtctga gtagtttttg cacagtaagg agtaaaaact
     3841 atggttaaag acccaataaa aaaagcaaag aatggaacct attattttcg tgcaaatcta
     3901 ggttatgatt ctaaagggaa aaaaattcaa aaatatcgta gtggattcac tactaaaaaa
     3961 gaagcaagag aagaatattc gaaactgcta ttgatgaaac cagaagaatt aagtgagaat
     4021 aaggataaaa tgacgtttaa acattatatc tttgaaattt tccttccatg gtataagaca
     4081 caggtaaagc tcagaacata tgaaaatcgc ttaccaacta ttaagaagca tttctcttat
     4141 tttgataaaa tggctgtttc cgatattgag cctattgatg tgcaaaattg gcaactaaaa
     4201 ttatcaaaga agtgtaagtc ttcttatgta agagctgttc aaggactatt ttcagttgct
     4261 atggatcgag caattgtcct tgggataacc accacaaatc catctaagat tattggcaat
     4321 gtgaaaaagc aaaagtctaa aattgaattt tggacaaaag aagaatttga aaaagtaatc
     4381 tctctaattt acaaagaaga ttactatcaa cactttttat ttatctctct gtggttttta
     4441 tttatgactg gaatgcgaat tggtgaagct actgccattc aatgggaaga tattgatttt
     4501 gactcaggag tactaaccat caacaaaaca ctctattaca agaatcaatc gaattataga
     4561 tttgttgaac caaaaacaaa ggcgagcgtt cgtcatattg tattagataa atgcacatta
     4621 acttatttat ccgaatggaa ggaagtacaa caaaatctaa ttcagacaga ctttgtaatg
     4681 agctataacg gtattcctac ccaaaaacat acactatcat atgctattga acgctatgct
     4741 aagctggctg gtatccatcg aatcagaatc catgggttac ggcattctca tgcttccttg
     4801 ttaatcagta tgggagaaaa tccacttatc attaaagatc gtctaggaca cgaagatatt
     4861 gagacgactt taggaacgta tggacatctt tatcctaaca gtaatttcga ggtagctaac
     4921 aaattaaatg gagccatttc ctttaaagaa gcaaaacaaa attatgatac ttcaccaaag
     4981 aatcaattta ctgtagacta cttgcgaaac aaagaaatga aaaaagtgca ataaaagtgc
     5041 agtaaaaaga taagaagagc cagaaaccct tggtataaga ggatttctag ctctcctaca
     5101 actattccca ctccactgtt gctggtggtt tagaagtgat gtcatacacg atacggttga
     5161 cgtgggctac ttcgtttacg atacgtacag agattttttg caagacttcc catgggatac
     5221 gggcaaagtc agcggtcatt ccgtcgattg aagttacagc acggataccg actgtgtagt
     5281 cgtaagtacg tccgtcaccc ataacaccga cagagcggat ccctggaaga acagtgaaat
     5341 attgccagat atcacggtca agtccagcag ctgcgatttc ttcacgcaag atagcatcag
     5401 attcgcgtac gatttctagc ttgtcttcag tgatttcgcc aagaacacga ataccaagac
     5461 caggaccagg gaatggctga cgccaaacga ttgaatcagg catgcctaac tgagtcccta
     5521 attcacggac ttcgtctttg aataatgtgt ttaatggttc gatcaattca aattgcatat
     5581 cttctggcag tccacctaca ttatggtgag atttgatcgt ctgtgctgtt tctgtaccag
     5641 attcgataac gtcagtatac aacgttcctt gtgctaggaa agagacgcct tcttcaccag
     5701 caagttttgt tgcttcgtca tcaaaaacat atacgaattc attcccaatg attttacgtt
     5761 tttgttctgg gtcagataca cctttcaact tgtctaagaa gcgatctctt gcatcgactt
     5821 tgatgatgtt caagccgaat tttccgccta gactttccat tacttgttcc gcttcgcctt
     5881 tgcgtagcag accgtggtca acaaagatag atgtcaattg gtcaccgatc gctttttgta
     5941 aaagaacccc aacgacacta gaatcaacgc cacctgaaag accaagcaag acttttttgt
     6001 caccaacttg ttcacggatt ttttctactt gcatatcgat aaagttatcc attgtccagt
     6061 taccttcaca gtgacaaaca tcaaaggcaa agtggcgcag caattcgatt ccgtattcgg
     6121 aatgacgtac ttctgcatgg aattggatac cataaaaatt acgtgttgtg tcttggatag
     6181 aagcaatcgg acaatctttg cttgttccga ctgtttcaaa gccttcagga actttagtta
     6241 ccaagtcacc atggctcatc catactgttt gttgttttgg tgtttcttta aacaagacag
     6301 cttgatcgct tgttacttct aacaaggctt tcccatattc gcggttttta gcaggctcta
     6361 cggaaccgcc aagtttgtac gtgatcaatt gcataccata gcaaatccct aatacaggaa
     6421 tacctaaatt gaagatctca ggatcgatcc caaatgcatt ctcatcatat acactgtttg
     6481 gtccgccaga aagaatgatt ccctttggat tcatcgcttt tacttcttct gcagtaatgc
     6541 ggtggctcaa taattcggaa aagactccaa gatcacgaat acgtcttgtg atcaattggt
     6601 tatactgact accgtagtct aatacgataa tcttttcaac gttcgtcata tcggcaacgt
     6661 ttgtcaaatt tattacccct atccctttaa attttatctc aaaatagtca aactatttta
     6721 aaatgctaat attttcgcat gaaaatcgaa tcgatgatat gattttcagt tttatggaga
     6781 attatatcgg ctcttcctcg tgtaggcaag atatattcct gtaaattttt caaattcaca
     6841 tttttccata catcttttgc cattttaaag gcttcttcac gatctccgat agcaaaacga
     6901 taataataat tctctggatt ttggaaagcc gtatccagta acgcaccaaa acgatccaaa
     6961 taccattttt cgatcagcac gggatcggca tcgacataga cagaaaaatc aaaaaagtcg
     7021 ctgacgtaga tttgctgatt cgcaggtaat tgaagcgtat tgatcccctc aacgatcaag
     7081 atatcaggct gctggatcaa ttcgtacttg tctggaatga tatcatacac atcatgtgag
     7141 taaacaggta cttttatctc ttcctgacca gattttacac ggttcaagaa atcgatcagc
     7201 atctccatat cataactttc aggaaatcct ttacggtcca tgattccacg ctcttttaat
     7261 atacgatttg gataaagaaa tccgtcagtc gtgatcagct gaacgtttct tcgtttgaag
     7321 gtacgagaca aaatcgtctg cagcagtctg gctgttgtac tttttccaac ggctacactt
     7381 ccggcgatcc cgataataaa aggcggtact tctacgtatt tatgaagaaa taaaccttta
     7441 ctcaagaaca aagattcaaa ttctttcata taaagataaa tcagatgagc aagaggtaca
     7501 tagatatctc gaacatcctg catggaaatg cggtcattca aacttttgat attatctagt
     7561 tcatcttgtg tcaaaggtgc ttgcccatta cgataaaatt ctcgccattc ttctcttgcc
     7621 acacggtagt agttcattgg atcattcata ttagcctcct gcgttctccc atcaatggaa
     7681 ctattttaac acaaaataaa cgcaaacccc accacttaag agagattgcc gatctttttg
     7741 tataatcttt ataatagaaa gaaacggagt cttattcttg acttgcgcac ggatacataa
     7801 aaggatagac tgttgtcatg aaagaaatga gaaggatgga agacatgaca aatcattact
     7861 attctgaacg cccagatacg gcgcacgatt ttgaacaatg ggcttttgag ttaaaaggca
     7921 gaacctttca atttgtaaca gattctggtg tattttcacg tgaaacagtg gattatggtt
     7981 cacgtgtcct gattgatgct ttcgattggg aagagttacc tgatgaagga aagatcttag
     8041 atgtgggatg cgggtatgga ccgattgggt tagctttggc atttgcgaca cagcggtttg
     8101 ttgaaatggt cgacattaat gaacgagcgg taagtctagc gcaaggaaac gcaaagcgta
     8161 accaaatcga acatgttgat atccatcaat cgaatatcta tgaagcagtt catgaagaaa
     8221 cctatgcagc tatcgtaagc aatccgccga tccgtgctgg aaaaaaagtt gtacatgaaa
     8281 ttttgacagg cgcatatcct cggttgaaga agggcggtac actgacaatt gtgatccaga
     8341 agaaacaagg agcgccgagt gcgcaaaaga aaatggaaga aacatttggt aatgcagaaa
     8401 tcgtcacaaa agataaagga tattatatca tcaaaagtgt gaaagaataa ctagctgaaa
     8461 aatagtggag cgacgaaaga gtggaactgg gcgtgcatgt acgggtacgt atcagttctt
     8521 tttttgttga gaagttttta aaaacaaaga actatttttt cttcaaaaag
//
LOCUS       DAZULY010000128         8569 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000128 DAZULY010000000
VERSION     DAZULY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.129"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..778
                     /gene="menA"
                     /locus_tag="ACG81A_001877"
     CDS             <1..778
                     /gene="menA"
                     /locus_tag="ACG81A_001877"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFS5015233.1"
                     /translation="NYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVI
                     GVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYV
                     NTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDI
                     ENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHL
                     DQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1592..2938)
                     /gene="gor"
                     /locus_tag="ACG81A_001878"
     CDS             complement(1592..2938)
                     /gene="gor"
                     /locus_tag="ACG81A_001878"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFS5015234.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3072..4325)
                     /locus_tag="ACG81A_001879"
     CDS             complement(3072..4325)
                     /locus_tag="ACG81A_001879"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFS5015235.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4331..5254)
                     /locus_tag="ACG81A_001880"
     CDS             complement(4331..5254)
                     /locus_tag="ACG81A_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFS5015236.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5475..7967)
                     /locus_tag="ACG81A_001881"
     CDS             complement(5475..7967)
                     /locus_tag="ACG81A_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFS5015237.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7974..8441)
                     /locus_tag="ACG81A_001882"
     CDS             complement(7974..8441)
                     /locus_tag="ACG81A_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFS5015238.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2354 a         1813 c         1421 g         2981 t
ORIGIN      
        1 taattacatg gattatgaaa aggctcattc tgaagtatat aagtatgaag aaaatgtcat
       61 tggccgcaga aatctatctc ctgtattgat tcgaaacatg attttagcta tgattgcatt
      121 tgtttttgtt atcggcgtta ttctaacgat caatacgggt tggcttttac ttttgatggg
      181 atttatctgt tgttttattg gagtattcta tacattcgga ccgattcctt tatctcgaat
      241 gcctttaggg gaaattttca gtggggtgac tatgggcctt ggaatttttg ccatggtcgt
      301 ttatgtcaat acattcggtt cagatgtcta tgatctgaca gtcaactttt catctggaca
      361 atttgatttg gtaggaaatc tttgggctat agcagcaatc gtattagctt ccttaccctt
      421 ggtgtttacg attgccaata tcatgttggc taataatttg cgggatcttg accgtgatat
      481 tgaaaatcat cgctatacac tagtttttta tatcggacgt cctgcgggaa cagtcttatt
      541 ccagctattg atgtatgctt gctatttcgt gattttaatg gggttgttga gcggtatcta
      601 tgaatggccg atcttggtca ccttcttgac cttgccaatt gtgaagaagc atttggatca
      661 attcaagcaa acgttacccc agcctatcag tttcagctat tccatcaaaa atatggtctt
      721 attcaatgcc agttatgtat tggggctatt actaacaatc attatgcaag ccatttaaca
      781 gcatgaagag gttgtgaaaa aactgccctg catcaagtaa taagaacagc caaataaaaa
      841 tagctcctta tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac
      901 cgtttatcag gaggttgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga
      961 aaatggcttt ccacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac
     1021 accgtttatc agaggttgta aaatcagtgt tttgtcctga gtattagaga aacaaatgga
     1081 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
     1141 actatttgtt cggaaaaagt gactaactat gacacaattt ttttcacagt cccttttttc
     1201 acttgatttg attcgtacca gcgaacttga cgagaacttt cttggtttgg taactatatg
     1261 gtattctttt aacatcctcg tttgcttctt ctaatttatt tatacaaagt cacacttttt
     1321 taaacatctg ttcttcgata catttctatt tcttttaaac atcgatacca atattttgaa
     1381 cttaagagtg cataccaaat ggattcattt actcttcgtc agttacgaag ataatcaaaa
     1441 tctccaactt ccctacttat ttcgttttga gaattatcga agcaatctta cataaataac
     1501 taaaaaacaa aaaaccagaa aatcagtata ctgattttct ggttttacat ggtttgttca
     1561 ataaaaagct gttggaactg ttaatattaa attatcgcat cgttacgaat tcttccgctc
     1621 ctgttggatg gatggctact gtattgtcaa agtcttcttt agttgcaccc atcttgatag
     1681 ccacggcaaa accttgcagc atctcatcga caccaacacc aatagcatgt aagccgacga
     1741 ttctttcttc ttttccgaca cagatcaatt tcatttcgca tttttggcga taatcattta
     1801 atgcaaagta cattggggta aagcgtgaac gataaacttt caattcattt tcgccatatt
     1861 tttccaatgc ttcttcttct gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga
     1921 ctgtcggaac aagtttatag tcaagataag aatctttctt tccattaaat aaacgttctg
     1981 ataaacgtct accagctgcg attgcaacag gagtcagatc taatttaccg atcacatcac
     2041 caacagcaaa aatatgttca tccgttgtat tttggtattt atctactttg ataaaccctt
     2101 ttttgtccaa ttccacattt gtattttcca gacctaattt ttcggtattc ggtacacgtc
     2161 caccggcaaa aagtacacaa tcaccagtta aggtttcacc attttcaaat agcaccgtat
     2221 attcgtcatt gtttttagta atttctttag caatatgatg tgcgtaagtt tttattccag
     2281 cttcttggta catttggatc aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc
     2341 gttcttttcg atatgcccac atgacttcac tacctaaacc acgaaataca cctgaaagtt
     2401 cagccgcaat gtatccagca cctaaaacga ttgctctctt aggtaactct gttaattcaa
     2461 aaaatccatt tgaatcaatt gcgtattctc caccaggaat ggtcatttta cttgcacggc
     2521 ctccagtagc gatcaagatt ttaggagcac gataggtttc accgtttact tcgatcgttt
     2581 gactatcgat aaaagtagcg taacctttta tagcctcgac atgattgcta tctaatcctc
     2641 tttgataagc accgtgtaga aaatcaatat atttttctcg tctttctact agaccggcga
     2701 aatcaacatt ttttaaatct gcttggatac cgtaacttga ggcatctcgt tcaattgttt
     2761 ccaaaatcgt acttgcttgc cacataactt ttttaggtac acaaccgacg ttgacacatg
     2821 ttccgcccaa ttcattcgct tcaatcagca aaacttttgc gccatgcatc cctgctctgt
     2881 ttgctgaagc aataccgcca gagccgccac caattacgat gtaatcatat atttgcattt
     2941 tgttcctcct tattgaagag atcaatcaaa acctttattt aacgatcctt tttctacaga
     3001 aaacaagtgt aacacttttc ttgaattttg tatgcgaaga tgcttaaaac agggatatat
     3061 cagctttttc cttatttacg ttttcggatc atatctccag gtttcaccgg ctgtgcgact
     3121 ggcatagtta aaatcatcat tggatttggt gctcggtcaa tggattcgcc ttcttcatta
     3181 tgcataactt caacggtttg actgaaatga ctgaatcctg gaccataaaa ttcgatttca
     3241 tctcctacag aaaacaagtt tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa
     3301 gataatactt caccgacaaa tttatatact gggattttac gacgttcacc gaataattgt
     3361 tcgttttcag ttggtgtatt gtagtaaaaa cctgtagcta attctcgttg tgctactttc
     3421 cataactcgt cgatccattc ttgcttgcag acatagtttt caggatcttc catataactg
     3481 tctacagccg ctttatagac atttgacacc gtagatacat aatggatcga tttcattctt
     3541 ccttcgatct tgaagctgtc tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt
     3601 gacatatcca ctgcactcat cgagaattct tcttccactt cgcctgtatc agttaaactg
     3661 tttcgttctt gaccaaaagg catatcgaac aatccatact tccagcgaca agattgagaa
     3721 catccgccac ggtttgcatc ccgcatcgac atatggttgg acaatgtgca tcgaccagaa
     3781 taagagatac acatggcacc atggataaat gcttcgattt cggtatccgt atttttacga
     3841 atctctgcca cttcctccat tgaaacttca cgagctaaaa cgacacgttc caacccttct
     3901 tctttccaga attccaatgt ctcaaaatta gttgctgaag cttgagtgga taaatggatc
     3961 ggcagacctg gggcttctgt cgcacaaatt tcgatcaaag ctggatcaga aacaatcact
     4021 gcagaaatac cgacatcacg caaactgcgg aaaaattccc ctgctccatc ttcatttcct
     4081 tcatgggtga ccatattagc agcaacgtat accttcgcat catgttcttt tgcaaaagca
     4141 actccttcag ccatttcttc ataagtaaaa ttacctgcac ggcttcttaa accatatgca
     4201 ttcccgccaa tatatactgc atctgctcca taatggatcg cggtttttaa cttttccagt
     4261 gttcctgctg gcgctaggat ttctggtcgt ttcaattttc tttttactgt catttctttc
     4321 atcatactcc ttatttgata gcagaaggat caatataaaa gaatcctgta tctaattcac
     4381 ggttttcagg atgcaattta ctgatttcgc tagtcatttt ttccgctttt tcttgatccc
     4441 agctaccttc taaaatttgt ttttttgctt cggcgaatag tcgagtgatg tttacgaatt
     4501 tttcgcctgg agtaaataat ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt
     4561 cattcaattc attcgagaga ttcagatcat tgctggcaaa aatatgtgtg ccatgactat
     4621 cttcataaat agaataatgt gtctcttctt ttttaggttc cgagataaaa agacctcttt
     4681 cacgatcttt ttgttcgtct tgtttcgtat agttatagta attctgtagc agtggacgtt
     4741 tggattgatg gatgcatgta gcaccataca caagggtttc tacaggaata tcgagttttt
     4801 cttccatcgc cttcatctct tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc
     4861 cttttttcgc ccagaaattg atttgacgtg cgctcgttac taatgtttca gcatcataaa
     4921 tatacggtat acgcaattca ggattttttt tcatcgtata aataatacct gtatcaccaa
     4981 ctgtgatcac atctacgttt atttctttta aaaaagccaa atattcaggt acatttttca
     5041 ttttttcagg atgaatgatc ccatttacag caatattcgc ttttttacca tacttgtgtg
     5101 ctaattctac caattgtcgc tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa
     5161 attgctcttc accgaaatat agtgtatctg cacctgcagc aagcaactgt tctgcttgtt
     5221 caatagtttc tactgtggtt atgagttcga tcataatttt ctcctcctac aacttcataa
     5281 tagtagcata gctctaaaaa ctttccaata cttcttctat ttaaagtcgg atttttgaaa
     5341 attatttcac aaaaaatcaa aataaagttc gatatattct gtccaaatat aaaaagagat
     5401 tgtgacaaaa atcttgtcac aacctcttaa tcaagtctta ctattataaa aattcagcta
     5461 cacaggccaa gtaactatag ggaacctact gtttcctttt taggtccgcg aacattcaat
     5521 gtgatcttac cttttgaagc acctactgtt acgtggtcac ctaaacgaat ttctccgcca
     5581 agcaaagcct cgctcaatcg atcttcaatt tctttttgaa gtgcgcgacg gattggacgt
     5641 gcaccatatt caggatcaaa tccagctttc ccgatcacat cgattgcaga tggtgtgatt
     5701 ttcacttgaa tatcttgttc tttcaatcgt ttgataatcg ctttgctcat gatttggacg
     5761 attgtatgga tttcatcttt actcaatgaa tggaagacga cagtctcatc gattcgattc
     5821 aagaattcag gtcggaaagc tttctttaac tcttctaaga tacgtttttg catggctttg
     5881 tgatctttag taacatcttg gacattgaat ccgacatttt tttcttctcg aatttgtgtc
     5941 gctccgatat ttgaagtcat aatcatgatt gtatttctaa agtccacttt tctaccttta
     6001 gaatctgtta aatgaccatc atccaatact tgcagaagta aattgaaaac gtctggatgt
     6061 gctttctcca cttcatctaa gagaatcact gaatatggtt tttgacgaat tttttctgtc
     6121 aactgtccac cttcatcata ccctacgtat cctggaggag acccgattaa tcggcttgta
     6181 ctgtattttt ccataaattc tgacatatca acacggatca gggcatcttc acttccaaac
     6241 atgacttctg aaagtgcttt ggccaattct gtttttccga caccagttgg tccaaggaac
     6301 atgaatgaac caattggacg atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca
     6361 cgtgaaactg ctttgacagc ctcatcttga ccaacgacac gttcatgcag aagtccttcc
     6421 agttctaaca agcgttcgct ttcttttttc tccaactgct gtagagggac accagtccac
     6481 tccgatacaa ctgtagcaac atcttcttct gttacacgat cagaataacc ttgctcctgt
     6541 tttgcttcaa ttgcagaaac tttttgcagc tttttacgta gtgctttttc ttgtctgcgt
     6601 aattgagcag cattttcaaa gtcttgtttt tggatcgctg cttccttttc ttgtactagt
     6661 tcatcgattt ctagtttgat cacagttgat tttgttaagt gatcggtttg atccaaacgg
     6721 actttcgctg ctgattcatc gatcaaatca attgctttat cgggtagttg acgagaagtg
     6781 atataacgaa cagaaagatt gactgctgct cgtacagctt catctgtgat ctccactcca
     6841 tggtgctctt catatcttgt acgtaatcct tgaagaatca cttctgcttc ttcaggagtt
     6901 ggctcatcta cttgaatgcg agcaaaacgt cgctctaatg cagaatcttt ttcgatatat
     6961 ttttgatatt catctagggt cgttgctccg atcgtttgta attctcctcg agctaacgcc
     7021 ggtttcaaga tattcgaagc atcaattgct ccttctgctc cacctgcacc aattaaggta
     7081 tgcaattcat caatgaataa gattacttga ccatcattat agatttcgtc gatgaccttt
     7141 ttcattcgat cttcaaattc accgcgatat ttcgtaccag caaccaaaga tcccatatca
     7201 agcatcatca ggcgcttttc ttgcatgtct tctggcactt cacctaaaat gattttttga
     7261 gctaaccctt cagcaatggc tgttttgcct acaccaggtt cacccaccag tacagggtta
     7321 ttctttgtcc gtcggctaag aatctggata agccgtttca cttctctcga acgaccaaca
     7381 actgggtcca gcttgttttc ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc
     7441 gtaggtgttc cttgcggtgt ttgccgttta tcttgtacat ttacatttct acgacgattc
     7501 attccttttc cagcagaatc accagctagc cccatttttt gtttcagaac ttgacgggct
     7561 tttgaaaggc tgatacctaa gttgatcatg attcgagtag ccatgatact ttcatctttt
     7621 aataagctca tcaaaatatg ttctgttcca actttttgag aaccagtttt cttcacttcg
     7681 ctgctagcta aagcaaaaac ttgtttcatt cttggtgaat atggaagaaa gactccttta
     7741 gggtaagaac ggataccacc ataaccaatc agatgttcaa tttcttcata aatttccgtt
     7801 tcattcaaat ccatttcacg taatactttt ccagcaattc cattttgctc gaccactaag
     7861 gctagtaaaa catgttctga actaactgat tgatgtttca accgttttgc ttcttcttgt
     7921 gcaatttcta atactgcttg ggctctttca gtaaaaagtt cttccatcta atctcacttc
     7981 ccatcttcat aacttaaacg ttccagaaat gctcggagaa tccgagcacg tgtatgatct
     8041 tcatattcat tgaaattcaa tgtgtttttt gcaattgcac tcaacatcaa gtttccttct
     8101 ttttttgtaa ttatttggtc ttcataaagt ttttgaataa tagcaaaagc atttttctca
     8161 ctgattgtat catcaaataa ctgatttatc tgctcaagta attgtttttt atctgaaatc
     8221 cttacttttg caattcgtat ataaccacca ccaccacgtt tgctttctac tagataacca
     8281 cgttggatgg taaaacgggt attaatcaca tagttgattt gtgaaggaac acagttaaag
     8341 aggtctgcca tttcaatcct tcgaatctcg atcatttcac tattttccag aattttcttt
     8401 atatatgatt caattaaatc cgaggtattc tgatgtgcca tcttattcat tccctttgac
     8461 taatattgac cttaattata acgaatttga taatagaaag aaagaagaac gctttttgtt
     8521 aaataaatcc tttagaatca acaaaaaaaa atacggaata tactattcc
//
LOCUS       DAZULY010000129         8351 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000129 DAZULY010000000
VERSION     DAZULY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.130"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            27..848
                     /locus_tag="ACG81A_001883"
     CDS             27..848
                     /locus_tag="ACG81A_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS5015239.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(1133..2143)
                     /locus_tag="ACG81A_001884"
     CDS             complement(1133..2143)
                     /locus_tag="ACG81A_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFS5015240.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(2155..3108)
                     /locus_tag="ACG81A_001885"
     CDS             complement(2155..3108)
                     /locus_tag="ACG81A_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5015241.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(3098..4168)
                     /locus_tag="ACG81A_001886"
     CDS             complement(3098..4168)
                     /locus_tag="ACG81A_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5015242.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(4174..5217)
                     /locus_tag="ACG81A_001887"
     CDS             complement(4174..5217)
                     /locus_tag="ACG81A_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5015243.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(5218..6159)
                     /gene="opp3b"
                     /locus_tag="ACG81A_001888"
     CDS             complement(5218..6159)
                     /gene="opp3b"
                     /locus_tag="ACG81A_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFS5015244.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            6243..6590
                     /locus_tag="ACG81A_001889"
     CDS             6243..6590
                     /locus_tag="ACG81A_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFS5015245.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(6660..8321)
                     /locus_tag="ACG81A_001890"
     CDS             complement(6660..8321)
                     /locus_tag="ACG81A_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5015246.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         2370 a         1719 c         1557 g         2705 t
ORIGIN      
        1 cttttactgt caatggaggt ggtcagatga accgttttgc ttctaaagaa gaagaaaaat
       61 tatattatga acataaagca agtgaagaag aatttcttaa ctggtatcaa aaacaggaac
      121 gtccagaata tgacaagccc tctttgactg tcgatatcgt actgatgtgc tataacaaag
      181 aagaagatca gctgaaagtt ttgttgatcc aaagaaaagg gcatccttat cgaaattcat
      241 gggcattgcc tggtggcttt gtccaaaagg atgagtcaac tggagaaagc gtcttaagag
      301 aaacaaagga agaaactggt gtggttattt caaaagaaaa tattgagcaa ctgcatacat
      361 tcagtacgcc taatcgagat ccgcgaggct gggtcgtcac agtcagctac ttagccttta
      421 ttggagaaga accgctaatc gccggagatg atgccaagga agtacgctgg ttcacactag
      481 aacgtcatag taacaagatc catttatcta gcggagaagt ggctatctcg cttgatttag
      541 tcactggaga gtcttctggc aaagatactt tggcttttga ccatagtcaa atcatcttga
      601 aagcattcaa ccgtgtagtc aataaaatgg aacacgagcc gcaagttcta caagttttag
      661 gtaaagattt cacgattacg gaagctcgaa aagtatttgc taaattctta gggatcgatt
      721 ataaaacgat cgatcattcc aattttaaga aagctatgct ccaatatttc gatgagatcg
      781 gtgaacgtcc tgtaggaatc ggccgtcctt caaaaatcta tcagctcaag ccggggcacc
      841 atgaataagg ttgtgagcaa aagattcaga cctaatcatt caagaaaaaa cgcaaaaaca
      901 actctttgtg agttacgttt tttgtttcag aattttgatg tattcgtaaa ctttctgaat
      961 aaaaaattaa caatagcaga aaaagctcgt acgaactttt tctgctattg ttttattggt
     1021 ttgtcataat tagtgagctt ttcttcaaaa gaaaaattcc tagaaacaaa agtcattaag
     1081 actttttatt aacaaggcta accctaacgc tcctatcagt ttcattcatt ttttatccct
     1141 tgataaccga atgtctgccc attttttcta ctacttggct ctttcttacg gaattgcttt
     1201 cttctgctcc gtcggtattg atcaaaagaa attttttatt ataccattgg gacttataac
     1261 gcaattttgt tattaattta tgccagtcgt gaatagaaaa atcagcatgt gcctcttctt
     1321 ttggaaaaga ttctacaaat aagtagtcat attgtttgac caatttccca gataattgtt
     1381 caagataatc ttctcgctgg ttcttttgtt tcagataaag atccattact tttctctttt
     1441 gtttctgata attgcttgcc tgatctaacc tgatctttct atggtgagcg gagcggactt
     1501 tcacattcaa tttccgctgt gcatgttgca gcttttcttc tgtctggtga aattttggta
     1561 aagttacttc tagaggtcga gatgtttgga tcaatgtctt cggatgatag gcgatcccga
     1621 tccatttagc taaagattct ttttttactg gcgaaacatc acaaagtagt gcgacataaa
     1681 attcttcatt atatcgagca gaaatcgtag cggagcgaat cgatccttca actggccgat
     1741 gctgatttat cttgattagt tctttttgtt ttggtaactt taagtagcca ttttccagat
     1801 agatcgtacg ggtttggttg ttggtcgtat aagattgcca catacttttt ttcgttttaa
     1861 gctttgggaa gctggcacgt cccttgaaat aattgcgaaa agcccgatcc aaattaagct
     1921 gtgcatttgc taaagcaaga ctgtctactt cttttaagaa tgggtattct ttttttaatg
     1981 tagcgggtgt gagcgaaaaa ttggctactc ctgtttgttg ccaacttttc tgccgctttt
     2041 tcagcaaatg gttataggta aatcgagcac atccaaaagt acgaatcaat tgctgttttt
     2101 gttcttctgt cggatacata cggaatttaa aagcttttag catctatgtc cctcctagtt
     2161 agcaaccaga agagattcct tttgtcgttt tagttttttt gcgtaatatg ggacttcctg
     2221 ctcagttgca tagacaaaat gttctggtgc gatttcacga agttgacgta cttgtccgtc
     2281 atctggttca ggttgatatt tgattcttct gcgttttctc tcatggtctg gatctggtag
     2341 aggaatagca gataacaaac tttctgtata aggatgaaca ccgtgattat agatttcatc
     2401 acttgttccc atctctaata acaagccgtt atgcattaca ccgattcggt cactaatatg
     2461 tttaaccatc gacaaatcat gtgcaataaa tagataggtc aattgatgtt ctttttggag
     2521 atcttgcaac aaattgacca cttgggcttg gatcgacaca tctagtgcag aaattggttc
     2581 atcacaaatg ataaaacgcg gttttactgc caatgctcgt gcaataccaa tacgctggcg
     2641 ttgcccacct gaaaattcat gaggataacg agtaccgtgg cttggattca atccaacagt
     2701 tctcaataga tcatcgactt tttttgcacg ctcttcagat gatttagcta gtccattaac
     2761 atcaattcct tcggcaatga tatctctgac tttcatacga ggattcaacg aagcgtatgg
     2821 atcttgaaag atcatctgaa catcatgccg gaatttcgtc aaaccttttt tatctttgat
     2881 cttcgttata tcttgaccgt cgaataatat ctcaccatca gtaggttcat tcaagcgaat
     2941 gatcgtacgg ccagtagtgg attttccgct tccagattct ccaactaaac cgaatgtttc
     3001 tccttcataa atgtgaaagc tgatgtcatt tacagcatgt acttcattct tttttcctac
     3061 attgaaatac tgtttcaatc ctttaacttc aagtagttta tttggcatca gcaattcctc
     3121 cttgaccgta agtatgtttt tgtttctctt taaagatagc ccagcgacgt tggatttctt
     3181 ctggcggtgt cactttgggt gcctgcggag caagtagcca agttgctgcc ttgtgtgttt
     3241 ttgatagttc aaaaaatggt ggttcttgtt ctgcatcgat tttcatcgcg aattcatttc
     3301 ttggatagaa tgcgtctcct tttggcgggt tcaataaatc cggtggagaa ccaggaatag
     3361 catacaatcg atcgttcgca ctatctaatg tcggcattga accaagcagt ccccaagtat
     3421 agggatgttg cgggttgtag aaaatttctt ccgatgttcc aacttcaacg attcttcctc
     3481 catacatgac agcgacacga tcagcgacat tcgctactac accaaggtca tgagtgataa
     3541 agataattga tgtatcgatt tttgtctgaa tttctttcag cagctccaaa atctgtgctt
     3601 gaatagtcac atcaagcgct gttgtaggtt catcagcaat caggatttcc ggataacata
     3661 ccagtgcaat cgcgatcacg atccgttgac gctgacctcc tgagaactga tgcggataac
     3721 tgttgatacg tttctcagct tcgggtattc caactaattt aagcaattct aatgcagctt
     3781 tttgtccctc tttttttgaa actttattat gctttctaag ggactcagcg acttgcttcc
     3841 caatgggcat cgtcggatct aaagaggtca ttggatcttg gaaaatcatc gctatttctt
     3901 taccacggat tttttgcatc tctctttctg attttccaac gatatcgtta cctttaaaga
     3961 ggatttgacc agcatctata ttggcgttgc ttgacagcaa gcgcatgatg cttctggtcg
     4021 tcactgattt tccacttcca gattccccaa cgatcgccaa tgtttctcct ttattcaaat
     4081 gaaaattgac accgcgaatc gctcgtacct ttccagcata cgtatcaaaa gagatttcta
     4141 agtctttcac ttctaaaatt ttcgtcatcg tcttcactct ttcattttag gatcaaaggc
     4201 atcacgcaat ccatcagcaa gtaagttaaa gccaatcatg ataaccgata aagttactgc
     4261 cggaatccat aatagataag gcagatacag gaatgtcttg tacccttcgc tcaacatcgt
     4321 tcctaatgaa gctgacggag gcgtaagacc taaaccgata aagcttaaaa aggcttcgaa
     4381 gaaaatagca gatggaatac taaacatcat ttgaataata atgacactag agatattcgg
     4441 caaaatatgt ttgaatgcaa ttttcattct tgattcacct aatgtcaatc cagctaaaac
     4501 atattcttga tcttttaatt tcattgtttg tgcacgaacg atacgtgcca ttggtatcca
     4561 gtttgtgatg gccatagcag ccacgattga aaaaattcct ggttcaaaaa cgaccaacat
     4621 caaaatcatg acaacgaggt ttggaattcc tgataagatt tccaaaaaac gttgcatcac
     4681 gttatcgaca cgtccgccta acaaaccaga aaccaacccg taagctacac caatcgttac
     4741 atctagtaat gcagcaataa atgcgatcag caacgagatt ctcattccca taaataagcg
     4801 gcttaataca tcacgtccca aaccgtctgt tcctaaaaag aaatttacgt ttgaagggac
     4861 gtttgcttga gcatatttat caacaagttc accaccaacc attgcttttc cattcaatcc
     4921 atcgatattg attcccggaa tacgaggagg caaattgata taagccacgt tttgggcagt
     4981 tggatcatga ggagaaacaa agattgtgat cacagagatc aaaatgatga ccaatagtaa
     5041 aaccattgaa ataacggcag ctttgttttt ctttaaacgg cgccatgaat cttggataaa
     5101 attcaaagaa ggtgcagtaa tctgttcacg ttcttcttcg gtattttttt gtaatggaag
     5161 aaattcatcc gcagggatat ctgcaactgt ttcataacgc tttggtatca tttccattta
     5221 tcctcggctc ccttctgaaa cacgaattct tggatcaact agaccgtaca atagatccac
     5281 gatcaaaata acaaaaatca gcataaatga gtaaaggatc gttacagcca tgatcgtagg
     5341 ataatcattt gttgtgattg acttaacgaa ttgttccccg ataccaggaa tagcaaagat
     5401 attttctacc accagtgatc cggtcatcaa tgctacagct aacggtccta ataaagtcat
     5461 caatggaatc aaactatttc gaagaccatg tttaaaggcg atttcccaac ggctcaatcc
     5521 ttttgctttt gctaattcca cataatcact atgcagtact tccaccattt ccgtacggat
     5581 aaaccgagca gagtcagcaa gcggcgacat ggccaacgca attgttggta agattgtgta
     5641 cacaaagcca tcccattttg cgattggcaa aacttgtaat ttgattgcaa agatatattg
     5701 taataaaaca gcaaatacaa agtttggaat ggaacgacct aatatcgcta cgagtgtaga
     5761 agatgtatct gcccatgtat tttgcttcat ggcagagatc gttcctaaaa tcgtccctag
     5821 gactgttcca aagataattg cctgtaaccc taactgcaag gatggtccga ttcttccagc
     5881 taacaaatgt gcgactggct ggtttttaaa ttggaaggaa attccaaaat ctccttgaag
     5941 caaattagaa agataaatcc catattgtac gataactggt ttatccaaac cgacttgttt
     6001 atttagcatc tcgattgttt caggactcaa acgttcctga ttcgtatatg gcgtgcccgg
     6061 cagcaattgc atcaagaaaa aggtgattgt tgcaattaac caaagcgtga tgatcatgaa
     6121 aaagacacgt tttaatacat atttgatata actattcact aatgtttccc cctagctttt
     6181 gacttctgac agatgtttcg ctacattatt agataatgaa ccaaaaataa aggagcaatc
     6241 aaatgaaaaa aaaatctatc ccctacgcag tagcttttct actgattctg gttattttaa
     6301 taaaaaatct catcaaccat tcctttacac tcattcaatt atctaatgat ctattcttat
     6361 ggtcattgcc gtttctgatt atcggcggtt ttttatgggt cttttcttcg ggtttcttcg
     6421 atcattttca gcgatcggtc catcttgcac gaacaaggaa ccgaaagaaa aaaccagaat
     6481 tttcctcgct ctcgtctgcc agctacggta tgtacagctt ttggctgatc attgccggaa
     6541 tactaattgc tctttctgct attttcatgc ttttttcttt gttaggataa gtgaatatga
     6601 gaacgaggtt gcgacatttg ccgcagcctc gttttttctc atgctcacgg tattaacgat
     6661 tattcttcaa cgtatgtcca tttgtaatcc catgaagcgc ctgccgcatg gtgaacgata
     6721 cctttgattt tttcattcac taggtgacct tctgcttttt ggtacaccgg aacgacaccc
     6781 atatcatcag agatgatctt gattgctttt tggaaatcat cccaacgggc ttgttcatcg
     6841 ccagcgtctg ttgatgaagc tgctttaact gcttcgtcat attctgcatt gctccagcga
     6901 ccacggttgt atgaattacc tgtgacaaac aaatcagtaa agctgcttgg gtctgcatag
     6961 tcagcagccc atccgcctag tactgcgtca aaatcacccg aagttgagcg gtcaagacgt
     7021 acagagaatg gaacaggtgt tggtttgact gttacgccat ccaagttttc ttggattgag
     7081 ttttgaacat attcgattac ttttttcgat gaatcatcat cagaagccat caaatcaaat
     7141 tctaaagaat caacgcccaa ttcttttttc gctttttccc aatattcttt tgctttatct
     7201 ttgtcataag aaactaattt tccagcttct tttgcaaagt cttcgttcgt tgttggactt
     7261 tttaccatat ctgatgggat caatcctgta gagacaactg atccgtcacc tagtacttgt
     7321 tttactaaag cttcacgatc gatagaataa gaaatagctt ttcttaggtt cacattgttg
     7381 aatggtgaat tcttttcacg ttgattgaat tcgatatagc ttgtccgcgc ttctttaaca
     7441 gaagtataag ctggatcgtt tgcgttttgc tgtgctaact caccagacaa gatcacgtca
     7501 tctgcttggc catctttgaa taagttcaat gcagtagaag attcttttac tacactgact
     7561 ttgatttcag aaagttttac tgtatctttg tcccagtaat gatcattttt cttataagtc
     7621 cattctgtat ctgttcctgg tccatcaaaa tcttctaatg tgaatggtcc attataaaca
     7681 gctttatcac ttgcagatgc ataggcatcc ccattttctt ccactacgga ttgtttttgt
     7741 gggaaaaatg atgggaaagc aagcagatat tggaaatatg gtgtttgttt cgtcaatttg
     7801 atttctaatt catagtcacc aactgctttg atccctagtt ctgatttatc tttctttcca
     7861 gctgtaatgt cttctgcatt ttcaactggc gcatacaggt aagcatattc agaagctgta
     7921 gctgggtcta ctgttcgttg ccagccataa acgtaatccg ctgctgtaac aggatcacca
     7981 tttgaccatt ttgcgtcttc tcgtaatttg attttgtaag ttaatccgtc ttcactgact
     8041 tctgccaact cgctagctcc tgccggctgt ggtttgctgt cttcatcaag acggtaaatt
     8101 ccttcataaa cgttattcaa tgctgtaaaa ctgattgtat ctgttgcaac tgataagtca
     8161 gctgtaggca tttcttgagg cacgactaag ttaaatacgc ctcctccatc tgctgaaccg
     8221 ctttttgctg aactactgct tgtggctgaa ctgccgccat aacatccagc aagtacaagg
     8281 ccgcataccg caaccaaacc aaatgtaaat ttctttttca ttttacgtac ccctttattg
     8341 gaattttcag a
//
LOCUS       DAZULY010000130         8297 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000130 DAZULY010000000
VERSION     DAZULY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.131"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(750..2381)
                     /locus_tag="ACG81A_001891"
     CDS             complement(750..2381)
                     /locus_tag="ACG81A_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFS5015247.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(2395..4068)
                     /locus_tag="ACG81A_001892"
     CDS             complement(2395..4068)
                     /locus_tag="ACG81A_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFS5015248.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(4081..4398)
                     /locus_tag="ACG81A_001893"
     CDS             complement(4081..4398)
                     /locus_tag="ACG81A_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFS5015249.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(4423..5823)
                     /gene="lacG"
                     /locus_tag="ACG81A_001894"
     CDS             complement(4423..5823)
                     /gene="lacG"
                     /locus_tag="ACG81A_001894"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFS5015250.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            6060..6845
                     /locus_tag="ACG81A_001895"
     CDS             6060..6845
                     /locus_tag="ACG81A_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5015251.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            6848..7540
                     /locus_tag="ACG81A_001896"
     CDS             6848..7540
                     /locus_tag="ACG81A_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5015252.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7814..8185)
                     /locus_tag="ACG81A_001897"
     CDS             complement(7814..8185)
                     /locus_tag="ACG81A_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFS5015253.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         2537 a         1819 c         1426 g         2515 t
ORIGIN      
        1 tagatagtat tctacctcat atttatttga aagtaatata taaaagacgt tcaaattcga
       61 acgcctctcg ttaaccaaat attggattca gaatctagtt ttcatatata aacagagtat
      121 gattaggtgc aaaaattgca cttttattgc acttttattt ttatttatag tgttttactg
      181 ttctgtatat tgttttaact cccttgatag tatcgcattc tgttttatac actttacgtt
      241 gaaaacagag tgggaataaa gagccaacat aatatggagg gggaatccct ataaatcaac
      301 attcttcaat agctaaaatg cttgattttc attaaaaata agatggttcc ctaccaaaag
      361 agactataat ataagttaat ttgtttcatt cttatcataa aaagaagttc tcgaaaaacg
      421 aactgttttc cgagaacttt tttctatcca tctaagtgct caaatcatat tcacgacgga
      481 attctaaggt taccctttct taaacaaact ttatgttatc tgcacaactt tttgcaattt
      541 tttcattgct tcttacgaaa tcgttgaact ctctttagca taatacaaag tgtagatgaa
      601 gtctggtgct ttactcgttt tggtaaaaat gactggaaat cgtttctggt tatttttggc
      661 aaaggcgagg tgcaggttcg attcctgcct tctacgctaa cgatgtggct gaagggaacc
      721 tccgatcttg aatcgaaacg atccgcagtc tattgtttca ccaatgaaac aaaatcaacg
      781 ttcgtccaag catttcctga agccttgcta taaaccccaa tttcacaaac acctgttgta
      841 attggaatgt ctttgattgt aatttttgtc cattcacttg ttttaacaac cgcagtattt
      901 agttccgctc caccataatt tttgatatat aactgattag tatcaaactc tcccccagac
      961 attacatagg cactcaatgt gtaagttcct ttgcttatgc cactaacatt ttgataagct
     1021 gatgcctcgt atgcttgatc ctgccaaaaa gttagtttgt agtccccaaa aaaagtgccc
     1081 gcttccgttt tgatcgtacc cttagcggtt ccttgttgaa accaggtttg ccaattggaa
     1141 ggactggtcg ttggtccatc gctttcgaaa cttgcatttt taactagatt tatttgcgcc
     1201 cctgcttgtt taaacgcacg caagcttggc aaggcgtttc cagcaaaatc aaacatcgct
     1261 tggttgtccc attcgtttcc catagtggat tgaacactga gataattcat acctgcttgt
     1321 gttgcccaac tcacatttcc gttgtaccaa agaggttccc aatagaagaa gcccgccccg
     1381 cgattattcg gtactgcctg aatccgatcg gcaagatcca gcaaatactc tgcttgtcct
     1441 gtcggtgttg gttcatatcc ccccgcagct gcttcggctt caccaaaagc atttggcata
     1501 gtatcggcat tcgctgttgt atgcccataa gctgtttcaa ctacgatcac ttctttacca
     1561 taccgttggc tgatcgcatt catattggtt tgaagttcat tcattgaacc gtgccaatac
     1621 ggataatacg acactccaat taaatcaaat gacacacctc gactcgtgat ttcatcaaac
     1681 caccaagtaa atgctcctgt atcgccacca tcagctaagt ggagcattac tggagttgtt
     1741 ttaggttgag tgtctcgaac agcttggata ccagacttca aaaaagcagc caatcgatca
     1801 aattctcccc cgccttctcc ccaagacttg ccataaggcc agagcattcc gctattcagt
     1861 tcattgccga tctgaaccat atcaggataa acgtccagct gcttcatttc cgacagaacg
     1921 ttctttgtat agtcgtacaa tgccgtattc atttcttcaa aggtcagacc ttgccatgct
     1981 ttaggaagat tctgtttccc aggatcaacc caaaaatcac tgtagtgaaa atccagcagc
     2041 accttaagtc ccagattttt tgctcgtttt gcaaggtcaa tcgtcgtttc cagatcattt
     2101 gtccctgctc cataactttc tccattacta ttataaggat cctgccacaa acgcagtcga
     2161 acatagttga caccattatt ttttaaaatc tctaaggcat cttttgaaat atcgtcttca
     2221 taatattttg caccagattt ttccatatct gacaggatcg aaatatctgc tccgcgaata
     2281 aattcgctgg cagatgcttc aacagttttt gaaacagccc ccaaagcccc taacacagct
     2341 gcaaataaaa cagctttacg caaccaaatt cccattttca ttatggctcc tcctttattg
     2401 aaattggttc agaataaact gaacagcttt ttcaggatct ctcgttaatc cgatatattc
     2461 tgctcctttt gtcgcaacca gcttgattcc taagcgatcg gtgtcttctt tgatatcatc
     2521 atagtaagaa ttgacttgcg gtgctaagac caccatatca aaatcctgca gcatttcgta
     2581 atgagaacca taagcacccg ccattgcact gatcggtgct ttcgattcag atgcaccttt
     2641 ttttaaagca ttagctaaca tcgcacttgt ccctgcgcca gcacaaagta ccaacacatt
     2701 gatttcttta tttgcatcgc tgataacttg actcgctgcc acaggtactt cctcacgtac
     2761 atcggcagaa gcaccttcct cggttgcgat cacctctgct ttttgtgctt ctttctctac
     2821 taagacagca tcgtatgctt tgcaaaatgg tagatagatc aggaagtcaa cgacgattag
     2881 aaccgctgct agaacaaaag ctaagatacc catgcccgtt cctagaacca aaccgatcgg
     2941 tgctggagtt gcccaaggca aaacgtacat aaagctgttc atgcccaata catcgacaaa
     3001 aaacttgaac aggcaaacgt ttacaacagg tgttaacaaa aatggaataa aaaaataagg
     3061 gttcaaaatc atgggtgccg caaatagcaa tggttcgttg acagcgaaac ttactggaat
     3121 aaaggaagct ttcccaactg ccttcaactg cttagactta gcaaagagta ggaagataaa
     3181 aggaacaacc aatgttgccc cagttcctcc catagttcct acaaaattcc ctaaacccac
     3241 cgtcagtact ttagaagctt gttcaccgga attgaacaat tgcagattgg tttccacatt
     3301 ggcatagata attgccgcaa ttgccggctc cacgatcgac ggtccatgaa caccgacaaa
     3361 ccaaaacagc gccatcgctc cccagataat agcgattcct aagtacccgt cagctgctgt
     3421 aaatagcggc tgcagcaccg taatgattgc ttctgcaaat gagatttgga agatttgtcg
     3481 aatgcctaaa tcaattaaaa cggccgcaaa tactgaaaaa gagaaaggaa aaatatctcg
     3541 gaaggtttgc gaaatcgttc caggaacttc tttaggcatt ttaatggtaa tatctttcag
     3601 cacacaaaac ttatataaat taactgttaa aaatgcagcg acaaaggaag ccagtagtcc
     3661 ctttgtcccc atatattccg ttgaaaatcc accttcaatc ggagagacgc ttaacaacag
     3721 gaaacttacc acagaggcca gcatcacaga ggtggtgttg attactttgt ttttcggtaa
     3781 gcgacgattc atcgaatcag ccaagcagcg agcagtggtt gctgaaacga gtaaccctac
     3841 aacacccatc gaataattat agactttcca tagccatgtg gagacagatt caggccattg
     3901 gtaaccaaaa atctcaggaa ccgcagcaat caaaatgaaa atgcttgaaa ataaaatagc
     3961 tggcatcgct gttaaaaatc cgtcacgaac ggctcctaaa taactattac gtgagatttt
     4021 ttcaaaaaat ggtttccctt tttcgatttg tttaatgatt ccatccatag cgttcttctc
     4081 ctatccttga ttctgtctgt acagtacaat aaagtcttga atcagatctc tcagcaataa
     4141 agtggtcatt aaatgatcct gcccatggat aaaaatgaat cccatctcca tctctttccc
     4201 agatgcttct tccgctaaaa tttttgtttg cgaattatgt gcaagggtta aattttcgtc
     4261 ggcttctttc aacgccgaat caacatgttc aaagtttcct tgtcttactt ctttcaataa
     4321 tttcaataat gtactgcgag cgtcgcctga atatgcaacg atctcaaaac caagcatttg
     4381 taattcctct ttattcatct tttcttcctc cattatcact tattaaataa tcgttttggt
     4441 ttcactgacc aacttgtacc agtaagctga ttcttttggg taacgcttct gggtttcaaa
     4501 atcaacataa aacaagccat accttttgtt atacccattt gtccatgaga aaagatccat
     4561 aagcgaccac aaaaaatacc ccttcacatt taccccggct gttatcgcct tgcttaagga
     4621 ttccaaatag actctcaaat agtcgattct cggctgatcc ataataatgc catcttcaaa
     4681 ctgatcttta taccccatcc cgttctcagt gatatagatt ttgttgtaat gtgggtaatc
     4741 gcttttaatt cgcagcaaca agtcatatag accttctgga taaatcagcc agtcccaatc
     4801 agttcgagga attccttctt tataaatacg ttctccgatt cccttcactt tataaacaga
     4861 ggtccctttt tcacccgtac cgttatgatg gattgcattt tctccatcgt aagctttgac
     4921 aaaatggcat tggtagtgat tgatccctaa gtagtcatta cgcgtggatg cttttttcaa
     4981 ttcaacgaaa tcttcttcag gaaaatgata gctggcctga tttgcttcac atatctcatc
     5041 aagagcagtc aaggtttcag ttgaataata gcctaaatag gtagcatcta ataagaaacg
     5101 gatcgacaat gcatcgtcta aaaaggcagc atgcttatct tccggcgcgt ctgttgccgc
     5161 atatttcgtc tctaatgagt gaactacacc aatttcaccc ggaagttcgt tttctttgaa
     5221 atagttcact acacgggcat gagcaaccat catattatga agacaggcga cgatctttgt
     5281 aaaatcatat ttgattcctg gtgggaaaac acctaacaaa tattgattgg ttgcaactgg
     5341 atagatttca ttaaaggtac tccacacctt gacttctttg aattcatgaa aacaaaaaat
     5401 ggcataggaa acaaatgctt ctattgtttc tcgattcaaa aaatctccat ggtcaaacaa
     5461 acgtttaggg gtatcaaaat gatgcaacgt cacaaacggc gttacgtttc ttttcttaca
     5521 ttcttcgaac acacgatgat aaaaagcgat tccttctgga tttggtttcc cagctccatc
     5581 aggaaaaatg cggctccaag caatggataa tcttagacca tttacaccaa aacgttcaca
     5641 caattcaatg tcctttgcat attgatgata aaaatcactt gccggatcgg gactgaatcg
     5701 cccttgttcc tctaaaaagt catcccaagc aacagcgcct ttgccacctt ccttagtggc
     5761 tccttcaacc tgataggctg ctgtggctcc gccaaaaata aaatcttctg gtaatttaag
     5821 catgtacatc tctcctcaaa tctataatca gttttcgctc tttctaactg attgcattgt
     5881 aaacgcatcc aaaacatttg tcaacaaaaa caaacacaaa taatcataaa aaccagaaaa
     5941 cagcaataaa atctattgta acaaacataa aaaccaccct aaatgtttac ttttgttcga
     6001 attagattga ctgttgtccg tttatatgat agattatcct taaattagag gtgaagacaa
     6061 tgttgaaaaa agaaagattg cttactatcg tagaaatggt caataaaaaa ggaatcctta
     6121 ccgttaatga aatcatcaat caattagatg tttctgatat gaccgttcgc agagatttag
     6181 atgaattaga aaaatcgggc aagctgctgc gtgtccatgg aggggctcaa agtctttcct
     6241 acactttaga tcaagaactt tctcacactg aaaaagccac tctgcagatt caagaaaaaa
     6301 aagaaattgc tgccacagca gcctccttga ttcacgaggg agaaacgatt ttcttaggac
     6361 caggtacaac aatcgaacta ttggcgacgc atttactgaa caagaaaatt cggatcatta
     6421 caaataatta tccagttttt gatattcttg ctcaagcaga tggaccagaa attctactga
     6481 ttggcggtga tttccggaaa aataccggcg catttgtcgg accgatcacc aacgacaatt
     6541 taagaagact aaagtttaca aaagcattta tcagttcaaa tggcgttcac aacgaagcga
     6601 tctctactta cagtaccgaa gaaggagaag cacagcagat cgctctaaat aattcccgta
     6661 agaaatatct actcgttgat aataaaaaat tcaatcgaga tgatttttat gtgttttata
     6721 atttgcatga tttcgatctg ctcattacgg atcgccagat ctcgcgggag gttcaagagc
     6781 actatgcaca atatgtcgat ctgcaaattg ctgattcaac cgaaaatacc actataggag
     6841 cgtaactatg aaagggatta tatttgattt caacggaaca atgtttttgg actctcatct
     6901 gcatgaagct gcttggctgc atatgataca tgatcataca aaagatggtt tatcagacga
     6961 ggatatttta aaaaatattc atggtcggac gaatacggag attttgaaac atttcatttc
     7021 agagcatttg accgcctctg agatcgccgt attgtcagaa gaaaaagaaa gctattatcg
     7081 tatgctttgc ttgcaaaatg aaaacgagct tcagttgaca aaagggcttg aagcgactct
     7141 tgaccagctg actgaccaac agattccatt aacgattgcc acggctactg tcaaagagaa
     7201 tgttgctttt tattttgata ttttcgattt agcccgctgg tttgactggg acaagattgt
     7261 ctatgacgac ggctctttcc ctgggaaacc gcaaccggat atttttctta aagcggcaga
     7321 caaactgagc ttagctccgg ctgattgtct tgttatcgaa gatgcttact caggattact
     7381 tgctgccaaa cgtgcgggga tcggaacaat tattgcaatt gatccattcg gtaaaaacaa
     7441 aacagtattt gaacaggcac agctaaataa agatggaacc atcaaagatt ttactggttt
     7501 ttggacagag cacctgtctg ccacatccgt taattcttaa acgattcaac taactaccaa
     7561 agaaaagcaa ctgaaatgca cacctccttt tataaaaaac tgccagtgaa acgacatgaa
     7621 agatacactg ctatatcctt agtgaaatat agcagtgtat ctttgacttt ttcattcttt
     7681 taaccatatg acgcgttcat ctctatctat aattaacatt aaaaatagtg ttttcacatt
     7741 atttctcaaa ccaatcttaa aaatcatcag gagtactttt ttgatatact tttattaatg
     7801 ctgggcgtaa tggttaaaag atttccacta gttcctctcc accaagctga gttgaaaaat
     7861 gcttatctgt aaacaacata gacaaagagg aaataaacgt ttccgtatct ttctctgtcc
     7921 caaggatatt aattaatttt cctactaggt aagtatcatt aaacttaaga tctgccttca
     7981 catcaaaagt tttgataaaa gcttttatca tgcgtacaat tttgtcttta tcgccataat
     8041 tgatttcacc actcatatca atatagtcac taatccctga agaaataatg gcaactgctt
     8101 cttctttcgc cttatcagat aattcattta atgactggga ttgattgatg acttcttgag
     8161 ttttatctgt tacgtaatct ctcattatcc tatcggcata ctccgcaaag taatcttgaa
     8221 aaaattcttc tacagggtaa ccaacttcgt tttcccaacc aggagaaatt tcattatcag
     8281 tacttattaa atctatt
//
LOCUS       DAZULY010000131         8288 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000131 DAZULY010000000
VERSION     DAZULY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.132"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..461)
                     /locus_tag="ACG81A_001898"
     CDS             complement(<1..461)
                     /locus_tag="ACG81A_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFS5015254.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLA"
     gene            complement(591..1301)
                     /gene="budA"
                     /locus_tag="ACG81A_001899"
     CDS             complement(591..1301)
                     /gene="budA"
                     /locus_tag="ACG81A_001899"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFS5015255.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1314..2978)
                     /gene="alsS"
                     /locus_tag="ACG81A_001900"
     CDS             complement(1314..2978)
                     /gene="alsS"
                     /locus_tag="ACG81A_001900"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFS5015256.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3186..3848)
                     /locus_tag="ACG81A_001901"
     CDS             complement(3186..3848)
                     /locus_tag="ACG81A_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5015257.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3858..4673)
                     /locus_tag="ACG81A_001902"
     CDS             complement(3858..4673)
                     /locus_tag="ACG81A_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5015258.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4934..5377)
                     /locus_tag="ACG81A_001903"
     CDS             complement(4934..5377)
                     /locus_tag="ACG81A_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFS5015259.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5511..5849)
                     /locus_tag="ACG81A_001904"
                     /pseudo
     CDS             complement(5511..5849)
                     /locus_tag="ACG81A_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5837..6214)
                     /locus_tag="ACG81A_001905"
     CDS             complement(5837..6214)
                     /locus_tag="ACG81A_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5015260.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6438..7631
                     /locus_tag="ACG81A_001906"
     CDS             6438..7631
                     /locus_tag="ACG81A_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5015261.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7797..8219)
                     /locus_tag="ACG81A_001907"
     CDS             complement(7797..8219)
                     /locus_tag="ACG81A_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5015262.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2433 a         1676 c         1407 g         2772 t
ORIGIN      
        1 tttgctaata aaagatctac atatttttct agaaatgctt gatcgatttc atcataccct
       61 ttcgttactc cgcaatcgat atctaaaaca ccgattaaat taccttcttt taccattggc
      121 actacgattt ctgattgtgc ggctgaatcg caggaaatgt aatttgcatg ggttttgaca
      181 tcatcaacga tcaatgtttc ttgtttttca gctgattctc cacaaactcc ttttcccatc
      241 ttgatacgtg tgcaagaaac tcgcccttgg aacggtccta aaatcaattc attaccatcg
      301 aataaataat atcctgcaaa gacagtctca ggcaaagtct cattcaacaa tgcagatgaa
      361 tttgctaaat ttgctaacca gttatcttct gcttctaata atccttgttg ctgtaataat
      421 aaaatttcgt atgcatcttt tttctgtttt tctgtccaca tgataaaaac cgctcctttt
      481 tgatctatgg agaagattat agctcagttt ttttgtaaac gctaggtttc ctcctcaaaa
      541 aaacgagata cgtcaggtta atcaccttga acgcatctcg ctgtcttttt ctattctgct
      601 tgttggattt ctgctgctaa atcagcataa tcaatcgttt cgtgaacaaa agtcggatca
      661 tcaactggaa aatgctgtac aagggacgag atgcggctaa tctcaacttt tccttcagcc
      721 acttcaaaat ccattacatg tccgccaaat gtatgtgtat catcgataaa atgcaggtga
      781 aatcccgctg cagcaactcc ttggaataat tcaggcgtaa agaacccgac taatgttcct
      841 tggatattgc tttgttggaa ttctggttga gactctgaaa tttttgctag tcttctgtaa
      901 ggtttttctt gcttcggcat gatacggatg tgcatatttt tgaataatcc agttatcttc
      961 actgccaaaa ataggttttc cccggcttct cttactaata tgtctttttt caattgttct
     1021 ccatctgcag tttttgagac agaaaaacta gtatctggcg taaaatctgt aattgctgca
     1081 tagggggtaa gttcacttcc atctaattgg gcaactgagc catctgatct tccttgataa
     1141 gctacgccat ctaaaaagat gacttcacca tcaagcccat gcaatgttcc caatcccaga
     1201 gttccttttt ctaatatatg agcaatcgtt tctgttccat ccatcaagcc ggccatcaaa
     1261 gctcctaatg tcccgtgctg gtataagatt ttttcagtca tgtcgacact tccttaatag
     1321 aattgatctg gtaagattgt ttctcccaat ttctcattat cacggtaatc aatcggaata
     1381 tcaataatta cgggtccttc tgtcgcaaat ccttcttcta aagctttttc taattcctct
     1441 acacttgtag ctcggattcc tttagcgcca aaagcttctg catatttcac aaaatcaact
     1501 ggaccaaagt ctacacctga cgcccgctga tatttcattt tttcttggaa ctctaccata
     1561 ttgtagtgtc catcattcca aatcaaatga acgatattca attttttacg taccgccgtt
     1621 tctaaatcct gggcagagaa taaaaatccc ccatcgccgg aaactgatac gatttgtgta
     1681 tttggacgca ccaatgcagc agaaatcgcc catggcaatg caacaccaag cgtttgcata
     1741 ccattactga ataacaaatg acgaggttca taacttctaa agtggcgtgc catccaaata
     1801 taatgactac ctacatctac ggtaacagtc atatcatctg ttacttttga ctgaagcgtg
     1861 ttaataactt ctaaaggatg caagatacct gcttctcctt ttgattgaac aatgtcccgc
     1921 tccgttaatt ttgcttgaag agatgctaaa tattcttttg catcttctga cacttgttga
     1981 ggctctaatg aacctgtcaa taaatcaaga gtggctgaga tatcaccgat caattctctt
     2041 tcaggctgca taaaaggatc gatttcagct ggcgcttcat cgatcacaat gatccgcgca
     2101 tctttttccg cattccagtt tcttgcttca tattcgattg gatcatagcc aattgcaata
     2161 actagatcac ttcgtttcaa aagcatatct cctggttggt ttctaaataa acccacacga
     2221 ccaaagaaat gcgcttctaa ttcacgtgag atcactccag ctgcttggaa tgtctctaca
     2281 acaggcaatg ctgtcttttc aactaattgc ctgatggcta acgtttcttt ttcacttgat
     2341 cctcgcatac ctactaatag taccggtagt tttgcttctc taatgcgttc agctaggtaa
     2401 cggatatcat ctgcagaagc agaacctaat ttaggatcgc tcaatggctt gatgacatta
     2461 ccttggacag gtgcatccac tacatcttgc gggatactga taaagctcgc gccttttttt
     2521 gaactcttag ccatacgata agcattggca atcacttctg aaattgtttc tggatcttgg
     2581 atttctgcac tgtattttgt gattggctgg aacagtgctg cattatccat gctttgatgg
     2641 gtcaacttca atagatcgct tcgttttact tgtccggcaa ttgctaaaac tgggtcacct
     2701 tctgctgtcg ctgttaccaa acctgtagct aagttactag caccaggacc acttgttgcg
     2761 ataactactc ctggctctcc tgtaatacgt ccgattgctt gtgccataaa tgcagcattt
     2821 tgttcgtgac gagtcacgat taattcagga ccttgatcct ctaattcatt aaatacacca
     2881 tcgatttttg cacctggtat accgaataca taatcaacat gatgattaat caaactttct
     2941 actacagatt tcgaaccttg tttcattttt tcagccattg aacatcgtcc tttttatgct
     3001 ttatttttaa ttattagtta gaaataatca caaataagtg atacagtttc taaacctgtt
     3061 tcatacaaaa gtatagcacg ttttatttca tttgtttagc catatgttac aaaaaatgaa
     3121 aaagaatgac agtttttaca taaaaaaaac tgtcattctt aattgaaagt ttcaatttat
     3181 aaagtctaca gatccatagt tctcatcaat ttacgattgt tgaagtaaat caaaccaaat
     3241 acgatagaag gacctagtag aatacaccat tggggcttta cgaaaaagaa gagaaacaaa
     3301 cttgtcaata gtaaaaaatc tagtaaactc acataccatt ttttcaacgt atagtagaac
     3361 gctatccgta gaacttgatt tggtttcgac gctggatttc tcacctgaaa ataccagcag
     3421 ttggtgctaa aggaaactga taaacaagca agtaaaacaa acaacggaat caaccatttt
     3481 ccataagaaa aacgtataaa aaagaaacaa tcaaaaatca ttatcacact aactatccaa
     3541 gcaaatagcc aataaaaaaa tccctttagc caaaattggc gaaacatctt tatgaacaaa
     3601 cgtactggtg aaaaactatt ttcttttgct aattcttctg tcatacctat catcacgatg
     3661 caacctggac caataaaaag taaagaaaaa ataaaggaaa agacattcct tccatctatc
     3721 gacaaaagga acgcagctgc tatatacaag atattgacac accaaaaaca aaggctactt
     3781 gccaatgatt gatacaacca ataaaagaat tccatataaa catttttgtc aaaacgctgc
     3841 tctttcataa tcacctccta ttccttcatt cctgatgaag cgatcccttc tacaaaatat
     3901 ttttgcatcg ctaaaaatat aaaagcaaca ggaagaacag aaacaactga ggcagccatg
     3961 attaatgcgt actctgcatc aaatagccca ataaacattt tcaaccctaa ctggattgtt
     4021 ttattttccg tagaagacaa ataaatgaat ggccccatat aatcgttcca tgtattaacg
     4081 aatgtgatga tcgtcaaact ggcaatcgcc ggttttgtaa gaggtaaaat gatcttgcga
     4141 tagattcccc actctgataa gccatcgatc cgcgcactct cacataaaga atctgggata
     4201 ctactgtaat attgcttcaa caagaacaca ccaaaagcat tgaacgcttg taatagcacc
     4261 aacgaccaca acgtatctgt taaacccact tttctcatca taatgaactg cgggatcata
     4321 taagactgcc aagggacagc aatcgtgccg atatatgcta aaaataaaat atcgcgccct
     4381 ttaaaattta gtttagaaaa cccgtaagca gcaaaactag aagtaaacaa ctgaatcaac
     4441 gtgatcacaa cgcttaaaaa agctgtattc ttaaaaaatg tcactaaagg aatacgagac
     4501 caaataacca gataattctc taaatgccaa gttttaggga tccattgaat cggaatcgtg
     4561 aacacttcat tatttgcttt gaatgaagca gaaaccatcc ataaaaaagg aatgatcgtc
     4621 aataaagcta atacaatcag cagaatcata cccaaccatt gaataaaggg catgtttttt
     4681 gaacgaattt ttgctttttt tctttcattt cgaacacctc caaagttata atcttcattt
     4741 ctttgtaatg attacaatcc agaacaacgg ttattatagc tgattcattt ccgttcttct
     4801 tatttttgta agcgcttatt taacaagtta tgtaaatctc tttatttttt tgatttaaaa
     4861 atgaaaaaat acaaaagcaa gactgtcata cgtctcgctt ttgtattgaa atttcttcga
     4921 ttagagatac ttattactgt tcaaaagctt ctttgaaacg gtgaatcaat tcattgacta
     4981 caggtgataa aatccgattc tttttccaga ctagcatgca gttcgtttca atcattgggt
     5041 caagcggaac gaatgtcact cgctttgaat gatgacttac agcaccttct atgactaaag
     5101 cagcacctac ctgattctca attagcgcaa atacattgaa aatcaaattg tacgttcctg
     5161 tgatcagcaa ctgctccttt tttatgccta accagtcagc aaacattcgc tgtatttctg
     5221 gacgctttga gatcaaaaga ggtacattcg caaagtcttc ttttgttatc acgtcttttt
     5281 ttgctaaaac agaattttct aaaatgagca atccccattt ttcctttctt ggtaacaaaa
     5341 tcgtttcgta tttctctgta tctactggat ctagcaaaat cgctaaatct aacaggcttt
     5401 tttctagtca ctccgtgata tcatcacttg ttccgctgtg gatatgaaat tgaacgctgg
     5461 gataatcacg gatcaacttt tctagtaata gtgccaatgt attcgaacta tcagcttcca
     5521 cacagccgat cgtaaatcgg ccgctgaata attgtttttt tctgttctcg aattcttgag
     5581 tggtttgatt cgttaaatct aaaatttctt ctgctcggct tttttagaaa ataacctgct
     5641 tctgtcaaga tcattttgcg gttttcgcgt acaaacagtt ctgttcctag ttactcttct
     5701 aactccttca gttgtcggct taaggttggt tgtgtaagat gtagaacttc tgcggcttga
     5761 gagattgttc cttcttctgc aattgtccaa aaatatttta gtaagcgtaa ctccatagcg
     5821 atgctccttg atttttttac ttgatacatt ccattttatt tctagataac acacgatcat
     5881 atttcttgat ttttccttgt aaacgttcca aacattcttg catttcagca atttttttcg
     5941 tcaaaagttc actttcttca atcaaaagct tacgccgctc aaatacagtt gcttctcctt
     6001 cgctagatag gcgaatatat tcaatcaaag actcgatcga taatccggca tttcgcatac
     6061 agatcgtaaa gtcgacccat tttaggtctt cttctgtata atcacggatt ccgccttttg
     6121 tccgattaac tggtggaatc aacccgattc gttcataata gcgcaatgta tcggccgaaa
     6181 tgcccttttc ttcactgact tgtttgatat tcatctgatc actctttctc ttaatttctt
     6241 gccactttac ttatgtattt tatttattct acaactttaa gtgtactcca agtcaatact
     6301 catttctatt aaaactaagc tggattatct atatcggttg tttgtgtatt atttcaaata
     6361 aaaaagtctt gtttttttat tcttttttgg atatactttt aacaatttag ggaaatttta
     6421 ggaaaagggg tataaaaatg acaacgtatc agcgaaaggt attagtatcg acatcggcgg
     6481 gaattgcttt agagaatatg gatattatgt ttttagcttt ttcattatct tcaatgatta
     6541 cttcttttca tattagtggt acgcaagcgg gtttcatttc aacgatcact aatttgggga
     6601 tgctcattgg aggtattttt tttggcatca tagctgataa atatggacgt gtgaaagtat
     6661 tttctcaaac ggtccttctg ttttcgattg cttctttatt gatgtatttt gcttcaaaca
     6721 tctacttggt ttatctcttt cgctttatcg caggtgtagg agcgggagga gaatatgggg
     6781 cgtgtatgtc cttgatttcc gaaagttttt caaaaaaaca aattggacga gcttcttcgg
     6841 ttgctggtat tggtgcgcag gtcggtgcgg ctttggctgc gattttagct gctgtgatca
     6901 ttccaacgtt tggttggaag atgttgtatg tcgtaggtgt actgccggtg ttgatggtct
     6961 tgttcattcg tcgcggcttg actgaaccaa aggaattcaa atctgtacaa tctgccgaaa
     7021 agaaaaccaa acttagcaga ctgtttgaat caaaacatct tgcttggcaa acatgtggcc
     7081 ttagtatcat ggtaactgtc caaattgccg gctattttgg gttaatgaat tggctacctt
     7141 caattatgca aaatcgatta gggctttcgg tttctgggtc ttcattgtgg atggtcagta
     7201 ctattttagg catgtctttg gggatgatga cttttggcat aatcatggat aaattggggc
     7261 ctaaactagc cttttctttg tttttaattt gttcatcctt atctgtctat ctacttgtgt
     7321 tagcccatag ccagtggagt ttggtagcag ccgctgtgat agtcggctac tttatcaatg
     7381 gcatgtatgg tggatatggc gcagtgatta gtagtttgta tccaacagaa atccgcgcga
     7441 cggcgaataa tttcgttatg aaccttggac gagctgttgg tggtttttct tctatcgtca
     7501 tcggtttctt gatggatcat tatagtttaa ctgccgtaat cttgtttttg agcggtattt
     7561 acctagtcag tctgctggtc ttgacaactc ttactggtgt tacagcatta agggaaagct
     7621 tgcagcattg atagttgagt ctgtaaatga caagaaagag gtgtggaaaa agcttgcatc
     7681 tgcggtaata agaccagcca aatcaaaaac atgagacact gtttccgttt ggctggtcgt
     7741 attacttgat gcagggcagc tttttcaact gctaatttcc ttctatctgg cggcgactat
     7801 tctttgacgt ccgtcatttc aaagtacttt actaataact tagcataacg aaaaactgtg
     7861 gatacactcg tatattgagc gtctgcataa tcttccaaat ttttctcagg atgttgatac
     7921 atgaattcaa tcaaatgata attatatgat tttctgtaga aatgaagata aataatgtag
     7981 attgggaagt tctcctgctt ttccatagaa aagcgccgat tattctcatt cttcaaaatg
     8041 accttatcag gatattgttg gaacaattct tctaaatctg ttagataatt caagatagac
     8101 ttacttgata ccttcaattc gttttgtagt tcttctattg aacaccaacg ttctgtttca
     8161 agcagtaaat gaatcatttt aattttcttt tgagaaactt ttgttaaaaa agtctgcata
     8221 ttttctcctc ctgtatctac tttactatac tacatacaag aaaaaaacgg atacaaatat
     8281 catattgt
//
LOCUS       DAZULY010000132         8209 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000132 DAZULY010000000
VERSION     DAZULY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.133"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACG81A_001908"
     CDS             476..1339
                     /locus_tag="ACG81A_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5015263.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACG81A_001909"
     CDS             complement(1468..3090)
                     /locus_tag="ACG81A_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFS5015264.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACG81A_001910"
     CDS             3339..3860
                     /locus_tag="ACG81A_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFS5015265.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG81A_001911"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACG81A_001911"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFS5015266.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACG81A_001912"
     CDS             5941..6759
                     /locus_tag="ACG81A_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFS5015267.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACG81A_001913"
     CDS             complement(6862..7059)
                     /locus_tag="ACG81A_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015268.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACG81A_001914"
     CDS             7228..7806
                     /locus_tag="ACG81A_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFS5015269.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACG81A_001915"
     CDS             7901..8038
                     /locus_tag="ACG81A_001915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015270.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2460 a         1480 c         1619 g         2650 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaata
//
LOCUS       DAZULY010000133         8205 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000133 DAZULY010000000
VERSION     DAZULY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.134"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      15..94
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            92..1285
                     /gene="metK"
                     /locus_tag="ACG81A_001916"
     CDS             92..1285
                     /gene="metK"
                     /locus_tag="ACG81A_001916"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFS5015271.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1415..2887
                     /locus_tag="ACG81A_001917"
     CDS             1415..2887
                     /locus_tag="ACG81A_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFS5015272.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            2970..3758
                     /locus_tag="ACG81A_001918"
     CDS             2970..3758
                     /locus_tag="ACG81A_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5015273.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3825..4493)
                     /locus_tag="ACG81A_001919"
     CDS             complement(3825..4493)
                     /locus_tag="ACG81A_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFS5015274.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4608..6320
                     /locus_tag="ACG81A_001920"
     CDS             4608..6320
                     /locus_tag="ACG81A_001920"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5015275.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6323..8092
                     /locus_tag="ACG81A_001921"
     CDS             6323..8092
                     /locus_tag="ACG81A_001921"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5015276.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
BASE COUNT         2510 a         1390 c         1809 g         2496 t
ORIGIN      
        1 atctgctata cttggttcgt tgcaagttcc cgaaaggatc cgcaggacgg agatgccttg
       61 taaccgaatt taactagggg gaatcattat aatggtagaa agacacttat ttacatcaga
      121 atctgtttct gaaggacatc cagataaaat tgctgaccaa atcagtgatg caatcttgga
      181 tgcaatttta aaacaagatc caacagcacg agtagcgtgt gaaacgtctg taacaactgg
      241 tcttgtttta gtatttggag aaatatctac gacggcatat gtagatattc aaaaagtcgt
      301 acgggaaaca ataaaagaaa ttggttatac acgtgcaaaa ttcggatttg atggagatac
      361 agcagctgta ttggttgcga ttgatgaaca atctcctgat attgctcaag gagttgatga
      421 agcacttgag atccgggatg aagataaaaa agacgtatta gatgagattg gtgctggtga
      481 ccaaggttta atgtttgggt ttgctgtaga tgaaacacca gaattgatgc ctttaccaat
      541 cgctttgagt catcgtctgg tacgacgctt ggcagatttg cgtaaatcaa atgaattaac
      601 gtatttacgt ccagatgcaa aatctcaagt aacggttgaa tatgatgatc aaggacaacc
      661 ggaacgcgta gatacaatcg ttatttcaac acagcatgat gatgcagtag ataatgaaac
      721 aattcgtcat gatgtcatcg aaaaagtagt gaaggaagtt attccagctg aattattaga
      781 tgatcaaacg aaatattata tcaatccgac tggccgattt gtcattggtg gtcctcaagg
      841 ggatgccgga ttaacaggaa gaaaaatcat tgttgatacg tatggcggtt atgctcgtca
      901 tggtggcggt gctttttctg gtaaagatgc gacaaaagtt gaccgttctg ccagctatgc
      961 tgcacgttat attgctaaaa acattgttgc agcagggctt gctcgcaaag cagaagtaca
     1021 gctagcttat gcgattggtg ttgctcaacc tgtttcgatc tcaatcaata ctttcggtac
     1081 tggaacggtt ccggaagaag aactgattgc tgcagtgaga gaaaacttcg atcttcggcc
     1141 agcaggaatc atcgagatgc ttgatttgcg tcgtccgatt tacaaacaaa cagctgctta
     1201 cggtcatttt ggtcgtacag atgtagattt gccttgggaa catacggaca aagtagatgc
     1261 gttgaaagca agtttagcaa aataagttat attcagctgg aacgatcgcc aagtggtggt
     1321 tgttccagct ttttttatgg cagttaacct cttgaaaaag atagtaatta tttatctatt
     1381 tttagatcct gtcataatga aagggaggaa taagatgaaa aaagaaacaa atgttacttt
     1441 ggtcacagtg agtattttca ttgcaacgtt catgacggct attgaaggta caatcgtaac
     1501 aaccgctatg ccaacgattg tcggttcatt acatggaatg gaaatcatga actgggtatt
     1561 ttcgatttat ttgctgacaa acgctatgct tactccgatt tacggaaaac ttgcggataa
     1621 agtgggacgg aaaccaatct ttatttttgg gacattattg tttattatcg gttcgttatt
     1681 ttgcggttta tctcaaagca tgattgggtt gattgctgct cgtgcgattc aagggatggg
     1741 tgctggggca atgatgccag tagctttgac gatcatcggc gatctttatt ctattgaaaa
     1801 gcgagcaaaa gttttaggtt tgaatagttc agcctggggg attgccagtg tttttggacc
     1861 gcttgcggga ggattcatcg ttgaaacgat cagctggcac tggatctttt ttatcaatgt
     1921 gccgatcgga attctattaa ttattttgat ttggctatat ttaaatgaac caaaaatcat
     1981 acatgagtct aaaccaatgg atatttttgg tagttttagt ttgatggcca tgcttgtctc
     2041 attgctgtta gggttccaat tattaggaga cgaaggaatg agtccgcttg tttctgcttt
     2101 gctgctaggt agtatcgtaa tgttaggtat ttttgttttt gccgaaaaaa gagctaaaga
     2161 tcctgtcatc atgatgcatt tattcaaaaa tccactattt gttgtggtca acttggctgc
     2221 agcattggct agtggttttt tgatgggaat cgatgtttat atccctatgt ggatgcaagg
     2281 tgtattaggg ctgaatgcag caattggtgg gctggtatta gcaccattat cactagtctg
     2341 gatggtcggt tctttccttg caagcaagtg gatgactcaa atgaaaactc attgggtctt
     2401 gagaattgga ctactaatcg cagctattgg gggcgcttgg ttgtatctta tgcctttcag
     2461 aagtaactat ttatggtttt ttgctatatc aacggtcatc ggtataggct taggtgtgac
     2521 catgactacc tcgactgtca gtgcacaaaa tagtgtatct cgtgaccagc ttggtgtagc
     2581 aacttctttt aatacgttag ttcgaacaat tggtcaaaca gtcatggttt ctatttttgg
     2641 tttattgata aatggggtaa ctaaacgaga acttgcagca gcaaatttaa caaatgatgc
     2701 agatatcatg aataaactgg tgaatccaca aacggctaaa tatattgatg cggatatttt
     2761 gttaccatta agaaaaattc tttatgatgg gttacattcc gtttatctag ttggcttgtt
     2821 actcgtgatc attgctttgg tcttaacgct tttcgttcat gaaaaaagtg gtaaaataaa
     2881 acagtagttt cggttaagat atgtacgtag ccgaatcatt caaatttaaa gttattcaaa
     2941 aataacttct tactaaggaa ggcgaaaata tggcatttat tcaagcaaat atttattcga
     3001 atgtgttaga aatggaagtc aatctaaatg tgatactacc gcaaacaaca aaaaaggtga
     3061 ttggcaccag ttcagagcaa gagacaacgg atatacctgt tttatattta cttcatggaa
     3121 tgggtggcaa tcatagtgta tgggaaagac gtacgtcgat tgaacgttat gtagcaaact
     3181 acggattagc tgtcatcatg ccatctactg atcttggctg gtacacagat acagtttatg
     3241 acatgaagta ttggacattc attgcagaag agctacctga gatttgtcat gaattattcc
     3301 cgcaattaac aagaaaaaga gaaaaaacat atgccgctgg attatcgatg ggcggatatg
     3361 gcgcgttaaa actaggcctt gcaaaatcag aaaatttcgc agcagtaatc tctctttcag
     3421 gcgctgtttc tgtaggaaat cgaatggatg atcttttgat ggttcgtaaa gcatcttttt
     3481 gggaagggat ctttggacca ttggatcaaa tcgaaggttc aaaaaatgat ccgcaatact
     3541 tattgaaaga actagtaaaa agcggaaaac cagttccaag attttatatg gcttgtgggg
     3601 aatctgattt tctttatcag gcaaatcagg aaatggtatc tgctatgcag gaacaaggct
     3661 tggcggtcac ctttgaacaa ggacctggcg atcataactg gatcttctgg gaccaatgga
     3721 ttcaacgtgc attggcgtgg ctaatggaag aaaaataaaa aaagagtctt aaaaagatag
     3781 ctcctataga gatcacaaac atagtgatct ctataggagc ttttttactg ctcgttttct
     3841 cttcttttat tttgttggca taagccttca atataggttt gtgcactcgt tagaaagagt
     3901 gctttttcat tcaattctgc atgggactgc atactttctt taaatccctc taattgattt
     3961 tcaactacat aattacgcat aaatagaact gtttctttca aaaattcatt tccgtccata
     4021 attttttgtc gaagctgctg ttcatgctgc tgatccatta actgtccaat caataaattg
     4081 tggagagagg caatggcctg atgactttgg gtgactgaaa atccttcacg aacaagtaat
     4141 tggatcattt gctccaaatg ttctaatcga ctcggaaaag cgggcacagt attgatttct
     4201 aattctgccc cacaagggaa acgtgtataa aggtcatagt aattttccat atactgcagc
     4261 aactgttcat tccaaggcaa agttggatct ggaagcgcta attcttcttc cattgtttca
     4321 gccattattt gcaagagctg ttgtttgttt ttgaaatacc aatataaagc aggtgcttga
     4381 acatgaattc tgtttgcgat gtttcgcatg gaaagctgcg aaagagcttg tgtttcagcc
     4441 aggatatcaa aggcggcctg aatgatgatt gcttgagaaa gctttatttc cattttatca
     4501 acctcgtgta ttgcattatc tttttaaaca tggtaaattg ttagtcggtc taattttaca
     4561 gtgttaaatt agtcctgtca atttaaacaa aggggtgtaa tcaacgaatg aaattaatct
     4621 tacgacatgc gaaaaaatat aaagtagctg tttttatttc cttgctgtcc gtagcagtca
     4681 tggttgcagc agcattatgg caacctaagt tactacagca agtattggaa gctattatta
     4741 cagaagataa tgatgaaatg aaaacaattg gtatttacct gatcagtctg gctttgctag
     4801 gattagccgc aggtgtaacg aacacgattt tctctgcaaa agtagctcaa ggcgtaagtg
     4861 cagatatccg agaagaagca tttcgaaaga tccagacatt ttcatttgga aatatcgaac
     4921 agttctctgc tggaaatctt gttgttcgtc taacaaatga tatcacgcaa atccaaaatt
     4981 tagtgatgat ttctttgcaa tcactttttc gaattccatt cttatttatt ggtgccttca
     5041 ttttagctat gatcacgatg ccgcaattat ggtggatcat cgtagcgctt atcgttacgg
     5101 tatttctgat tacggcactt tcatttacaa ggatgggcaa gcacttcatg atcattcaaa
     5161 aactgattga tcgtgtgaat agcattgcaa aagaaaactt actagggatt cgtgtagtga
     5221 aatcttttgt acaagaagaa aaccaattga agtcattcag caaagtaagt gaggacttaa
     5281 caaaacacaa catcatagtc ggtacattat tttctgtgat gatcccgtcc ttcatgctgg
     5341 ctgccaattt ggctgtggtt ggggcgatct tttttgtgag cgatcttgtt aaagatgatc
     5401 ctacgttaat tggtggaatt gcttcattca tgaactactt gatgcagatt atgatggcaa
     5461 tcattattgg cggaatgatg atgatgatga cttcaagagc agccgtatcg ctgaagcgga
     5521 ttgcggaaat tttagatgct gaaccagatc ttacttattt agacgttcca gaacaagagt
     5581 tgacgggttc tgtcactttt gaccatgttt cttttcgtta tcctggtgag gatacagata
     5641 cgcttaaaga tatcagcttt tctatcaaac ctggagaaat ggttggtatc gtcggggcga
     5701 ctggtgccgg gaagtcaaca ctagcacaat tgatccctcg tctatttgat ccgacagaag
     5761 gcaaagttga agtaggtggt gtagatcttc gtcaagtaaa tgaaaagagt ttaagacaaa
     5821 cggtttcctt tgttttgcaa aaagcaattt tattctcagg aacgattgct caaaacttgc
     5881 gccaagggaa aaagaatgca acagaaaaag agatggaaca tgcttcttct attgctcaag
     5941 caaaagaatt catcgaaaag ttatctgatc gttacgaagc accaatagaa gaacgtagca
     6001 gtaacttttc tggtggtcaa aagcaacgtt tatccattac tcgtggcgtt atcggtaatc
     6061 caaagatctt gatcctagat gacagtacga gtgcactgga tgctcgctct gaaaagctgg
     6121 tccgagaagc actcgatcga gatttgaagg gaacaacgac gattgtcatc gcacaaaaaa
     6181 ttgcttctgt tgtcaaagca gaccgcatct tagtattgga tgaaggcaga ttagtcggcg
     6241 aaggaaccca tgaggaattg gtagctggaa accgtgtcta tcaggaaatc tttgaaactc
     6301 aaaaaggaac ggaggagtaa gtatgactga tctaataaaa gcaagtaaat ttttctatca
     6361 ctatttgaag cggtacaaac tctccttttt ctttatcttt atcacagtga tcattgctac
     6421 ctatctacaa gtaaaggcac ctcaatacat cggggaagct tttcaagaat tagctaatta
     6481 cgtaggaggc ttgatgcaag gcgtagacga taaaagcaaa tttgtttctg taatatggaa
     6541 actcttgctg ttttatgtac tagctagtgc agccaacttt atctatagta ttttgttcac
     6601 acaagtagta gggaagtcaa cgaaccgaat gcgtatcggt ctattcaaca aattggaaaa
     6661 attgacgatc cgtttctttg attctcatca agacggtgaa attttaagtc gttttaccag
     6721 tgacttagat aatattcaaa atagtttgaa ccaagctttg cttcaagtca tcacgaatgc
     6781 tgttttactt gtcgggatct tgatcatgat gttccgccaa aatgtagagt tagcttgggc
     6841 tacaattgct tccactccag tagccgtttt gatagctgtc ttgattatcc gcaaagcacg
     6901 gaaatatgtg gatcttcagc aagatgaagt ggggaaacta aatggttata tggatgaaaa
     6961 aatcagcgga caacgtgtca tcatcacaaa tggcttacaa gaagaaacga ttgaaggatt
     7021 cttagagcat aatcaaagtg ttcgcgaagc aaccttcaaa ggacaagctt attctggtct
     7081 actatttccg atgatgcaag gaatgtcact ggtcaataca gcgatagtta tctttttcgg
     7141 cggctggtta gcattaaatg gtgatttgga acgttcggtg gcattaggct tagttgtgac
     7201 attcgtccag tattcacaac aatattacca accactgatg cagatttcat ctggatacag
     7261 catgatccaa ttagcgatta caggggcaag acgattgaat gaaatatttg atgaaaaaga
     7321 tgaagtcaaa ccagaaaact gtaaagcatt tgatggtgtt gaaaaaggtc ttgcgttgca
     7381 gcacgtagat tttggttatt ctccagaaaa actagtcttg aaagatgtct cgatcaatgt
     7441 ggacaaagga gaaatggttg ctcttgttgg tccaacagga tcggggaaaa cgacgatcat
     7501 gaatctgcta aaccgttttt acgatgtcaa tgatggttca gtagtcatcg atggaataga
     7561 tatccgagat tatgatcttg atgcattacg tagacatgta ggaatcgttt tgcaagaatc
     7621 tgtattgttc tccggaacga tcaaagacaa tatcacattc ggcaaaccgg acgcttcaga
     7681 tgaagaagtc atcaatgcag caaaacaagc gaatattcat gacttcatca tgacgttaga
     7741 aaaaggttat gatacagaga tatccgaaga aaataactta ttcagtacag gtcaaaagca
     7801 gttgatcagt attgctcgaa cgatcatcac aaatccatct ttattgattt tggatgaagc
     7861 aacaagtaat gtagacacgg taacagaagc aaggatccaa aaagcaatgg atgaagcaat
     7921 caaaggaaga accagctttg tgattgctca ccggctgaaa acaatcttga atgcagatag
     7981 aatcgttgtc ttgcgtgatg gggaagtcat tgaagaaggc agtcatcacg agttattagc
     8041 agaaaatgga ttttatgcgg aactttatca taatcaattt gtatttgaat aaagaggttg
     8101 tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc ttctcatgtt
     8161 tttgatttgg ttggacttat taccgcagat ataagctttt gagca
//
LOCUS       DAZULY010000134         8194 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000134 DAZULY010000000
VERSION     DAZULY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.135"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            334..714
                     /locus_tag="ACG81A_001922"
     CDS             334..714
                     /locus_tag="ACG81A_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFS5015277.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1070
                     /locus_tag="ACG81A_001923"
                     /pseudo
     CDS             696..1070
                     /locus_tag="ACG81A_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1233..1637
                     /gene="tnpA"
                     /locus_tag="ACG81A_001924"
     CDS             1233..1637
                     /gene="tnpA"
                     /locus_tag="ACG81A_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFS5015278.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            1654..2802
                     /gene="tnpB"
                     /locus_tag="ACG81A_001925"
     CDS             1654..2802
                     /gene="tnpB"
                     /locus_tag="ACG81A_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFS5015279.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            2962..3501
                     /locus_tag="ACG81A_001926"
                     /pseudo
     CDS             2962..3501
                     /locus_tag="ACG81A_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3746..4435
                     /locus_tag="ACG81A_001927"
     CDS             3746..4435
                     /locus_tag="ACG81A_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015280.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(4556..4642)
                     /locus_tag="ACG81A_001928"
     CDS             complement(4556..4642)
                     /locus_tag="ACG81A_001928"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS5015281.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            5318..6268
                     /locus_tag="ACG81A_001929"
     CDS             5318..6268
                     /locus_tag="ACG81A_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS5015282.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            6289..6822
                     /locus_tag="ACG81A_001930"
     CDS             6289..6822
                     /locus_tag="ACG81A_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS5015283.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            6838..7008
                     /locus_tag="ACG81A_001931"
     CDS             6838..7008
                     /locus_tag="ACG81A_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015284.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            7458..7991
                     /locus_tag="ACG81A_001932"
     CDS             7458..7991
                     /locus_tag="ACG81A_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015285.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            8003..>8194
                     /locus_tag="ACG81A_001933"
     CDS             8003..>8194
                     /locus_tag="ACG81A_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015286.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKS"
BASE COUNT         3010 a         1407 c         1515 g         2262 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta acagaattag
     1081 gcagaagtcg cccatcctct ataggtgggt gatgaatgcc gttcggtatg agggttaccg
     1141 ttggtaactc gaagaccgac aactaaggcg tattgccttt ttattttgtt ccatgttaca
     1201 atcagtctat acaagaaatg agttgatata acatggaatt agatacaaat aatcattcag
     1261 tatttcttct ttattaccac cttgttttag taacgaaata tcgtagacaa gtgattgatg
     1321 atgaaatatc tgattatgcc aaaactactt ttgaaagaat ttcagaatcg tatcacatta
     1381 ctttagtgga gtggaatcat gataaagacc acgttcatat tatgttcaaa gctcaaccta
     1441 aaacagaatt gacaaaattc atcaatgcct ataaaagtgc cagctcaaga ttgataaaac
     1501 gtgactttcc aagagtcaaa caatttcttt ggaaagaaat gttctggtct aaaagttttt
     1561 gtcttctgac aactggtggc gcaccaattg atgtgattaa gaagtatatc caaaatcaag
     1621 gaaacaatca taaatagaaa gtaggtgaac tttatggaac aactaaaagc atacaaattc
     1681 agaatctatc caacagagga acaagaaatc ttctttgcca aatcttttgg ttgtgtccgt
     1741 aaggtctaca atctaatgct tgatgaccga aaaaaggcgt atgaagaagt taaaaatgat
     1801 tcttctaaaa aaatgacttt tccgacacca gctaaataca agaaagaatt tccgtttttg
     1861 aaagaaattg atagccttgc tttagccaat gcccaactta atttagataa agcatacaag
     1921 aatttttttc gggataaatc cgttggattt ccacgtttca aaagtaagaa aaatcctgtt
     1981 cagagttata caacgaataa tcaaaatggt acagttgctt tgattgatag taaattcatc
     2041 aaagttccta aattgaagtc attagtaaga attaagcttc atagacaacc aaaagggatt
     2101 atcaaatccg ctacaatatc acgtcattct agcggtaaat actatatttc tttgttgtgt
     2161 aaagaagaaa ttatcgaatt acctaaaact aattccacaa ttggtattga cttaggtatt
     2221 atggactttg cgattctttc tgatggacaa aaaattgata ataataaatt cacgtccaaa
     2281 atggaaaaga aactaaagcg tgaacaacgc aaattgtcta ggcgtgcttt attggctaaa
     2341 aataaaggta ccaatctatt tgaagctaaa aattatcaaa aacaaaaacg caaagtagct
     2401 agattgcatg aaaaagtaat gaatcaacgt actgattttc tgaataagtt gagtacagaa
     2461 atgatcaaaa atcacgatat tatctgtatt gaagacttaa acacaaaagg tatgttgcga
     2521 aatcataaat tagctaaaag tatttctgat gtttcttggt caaagtttgt gactaaatta
     2581 caatacaagg ctgactggta tggacgaaaa atcatcaaag tagataaatg gtttccgtct
     2641 agtcagattt gttcagagtg cggacataaa gatggcaaga aatctcttga aattcgagaa
     2701 tggacttgtc ctgtttgtca tactcatcat gatcgtgata tcaatgctag tatcaatatt
     2761 ctgaccgaag gtctaagact acattctatg ggattggctt agatccaatc atcaagaacc
     2821 gtaggaacta cggggatagc ttggtaaata agagacaccg ctgttagtaa agaaatacgc
     2881 tatcaagtat gctctattcc caagaagctc ccacttcaag cgttaagaac cgtcaggttt
     2941 agctaagtgg tgagtagttc acacaaatgt tatgacctaa atgacgagat ttgtcttgaa
     3001 catgacttaa caatcacgga acgaggaaag aaaaccgaaa aacgaaccat gtctgaaatt
     3061 aaattaaaag aaaaaaatga accggtttgg aaagacgaaa ttcggtttgc gattgatcaa
     3121 acaatgaaaa acaaaaaaac tcgaacgtat acccaatttt gtgattcact taaaacattc
     3181 ggcattcatt gcttgaatcg cggaaaaaat tttacttacg aattggtcca caaaaagaaa
     3241 aaagtacgat ccaacaaact aggaaaggat tacgaaaagg agacgatttt acatgagctg
     3301 gacagacgag aacgaaccta caatcaacaa cgaaccaacg agctacgaag agctcgagct
     3361 agcattcgcg aaactgcaga agaagtacga agagaacaac gaacttttac gcctgaacaa
     3421 tcggaaatta gaccgactcg aagaaaccat gaacaacacg ttgaacgagg aacagaccac
     3481 caacaagaat tatcgctcta aaacgttaaa agacatcgaa aacgaaataa agagacaacg
     3541 tgcctttctt gcgccttata cccaagaaag gatacaaact acgatggatt cacaagtcaa
     3601 aaaactccac caagcgctat tagaccagcg aaaccaagaa caaaaaaagg ctttaattca
     3661 acgatcgatg gatattcttg cccccttatg ttgcttagta ctaacattat tagccttttg
     3721 gttggtatgg ggagaataaa cagacatggc attctattta ccctatctac tcatttctgc
     3781 cagtatttta ggaattattt ggcttagcta taaggcttat caaacaagat attcattaaa
     3841 aggctctaaa attcgattta agcgattttt attggtcggg gttatatttg ccttatcaat
     3901 cgttttaagt ggtttattcg gcgttttaga gggtaataaa cgtgtttcaa gaagcatttt
     3961 attaggggat atcactcaaa agtatgaagc agcatgtgat aaaaagaaaa aagagcaagc
     4021 attagcacaa aaaatggagg aatttaccgc ttgttatgag gacatgaaca atatttttgt
     4081 ggatcaagag aaacgtttaa cagataaaaa catgaaaaaa ctcactcgtt tttatcaaaa
     4141 cttaccagaa aaacagcaaa aagaggtaca agaaaaatat gagcaagtaa aaaaagacgt
     4201 gcaatatttt aaagatacgc agacagaaga agcttgttgt aacttattta gcgatacaat
     4261 ccctttttca accagtgaac aagaacgaaa agaacggcaa caatcggtca catacgaacg
     4321 ttatgaaaca ctgcttcagc aagcaacaaa catacaaaat ccaacgaaaa aagaaacggc
     4381 attaaattat ctagaaagtg tcaaggaatg gttggatcag caacaacaaa attgaatttg
     4441 attagcaatc atgttaaact gaacctgcaa atgatcaaac attagataca cgaaagcccc
     4501 agagaggtcg gattctctgg ggcttttttt caggttgatg tcgcctttat tgattttact
     4561 tcttgtttcg gttattcaac caataactga ataaagagac acaaacttct acaagaaggg
     4621 ggcaaatgaa ccgttcaaac attagataca cctccttcca gtcgcaagac tgtcggatag
     4681 gcgacaagtt attatacctt acaaaataaa atctgtgtgg tgtttttaag tatcccttat
     4741 tttcgggttg ttagggcttg ccctgaccgc ctgtaaggcg ctttaagaac aagataaggt
     4801 tttttaccca agactgtctt aaaacgactt agacgagcta tcagaggctt cccccacctc
     4861 cgttactgac ttgtttttag caaaggagcc tttaggcgac tgcgcagtac aatgtgagag
     4921 catagcgatc acactttttt agcgaccgta gggagcaagg ttttgaattg gcagttttga
     4981 aaaatgcgcg aacctgttgg tagaaaaaag aagtggtttt taggcgggaa attttgtcac
     5041 cctcttttat aatctctttt taaacctttt tttaaacctc tttaccgtcg gctgtgtcct
     5101 tactccttca atggatacag cgttttataa actacatttt gtctgctttt aaactacatt
     5161 ttgtctgctt ttaaactaca ttttgtctgc ttttaaacta cattttgtct gcttttaaac
     5221 tctttttttg cactcacgta tgttataata tgcattatca cagacaaaac gttgtttata
     5281 acgtctgtat ccattgaagg agtaaggagg aatatctttg gcaaatgaaa tcgtgaaata
     5341 ccatcatgaa ttaaatacca ttccgttgcg gaaatttaca ccagttgaaa tgaatctatt
     5401 tttctctatt gtgtctagaa tgcgcgaaaa aggcgataaa acagtccgtc ttacttttga
     5461 ccaactaaaa gatttaagta attataaagc aactgctaat aatcgtttca ttaatgattt
     5521 agaaatgact tatgataaat taatggattt acgttttggt aaaagaagtg ctaatgggct
     5581 cgatatagat agattcgtta tgtttagtga gtttaaaata cgtggaactg ctaaagatcc
     5641 atatgtagac atacaagttt acgaaaaagc tttaccttta ctaaacaatt tagatgagtg
     5701 ggttcgctat tcactccaac aatttaacga attacaaagt agctatgcaa agactatgtt
     5761 tcgattacta aaacaatatc gaactaaagg ttttgcttat ttttctaaag aggattttca
     5821 tgaactatta gatattccaa aaagttactg gaacaaccaa gcaaacgttg ataatcgtgt
     5881 tataaagcct atacgacagg aattaaccgc aatcttcaaa ggattatcaa tcaaaaagaa
     5941 atacggaaaa gggcgaggaa aacctgtcgt tggctatcaa tttacgttta aaccagaaat
     6001 caaaaaagca gatgatttta ataaacatgc accaaccaaa gaagaacaaa ataaagcatt
     6061 ggctttagct gataaacaaa aagaatacga agaacctgct acaacaatta acgaaaaaat
     6121 caaacagaag aaacgagaac ttgaactcct ccaacaaatc aaacaactag aaaaagagaa
     6181 agaagctctt gaaaaacaaa cagagtccgc actttcgaaa aaagagaaaa aaagtttatt
     6241 atctgattta gataatttat tccagtagca acctaggagg caaccagtat gcaaccacaa
     6301 gaaaccttta caataaagga attgtcagaa ctttttaaga tgagtaggca agcaatatcg
     6361 aaacatatcc aaaaattaga ttcttccatg atagctaaaa atgagcgtgg ttataaggtt
     6421 gtattgcgtt ctggagtatt gcaacttgct cgcaacttag gcaaccaaat gttgctagat
     6481 aggttgcacg agtacgaaca agacaacgat aaaaatgtta atataccaag caaaaacgtg
     6541 gttacaaaca agttgctaga tcagttgcgt gaagaaaatg aatttttacg agaacaactt
     6601 gccctcagag agcaaaataa ttctcatata caaaaactac tcgaccagca acaacaactc
     6661 accctacaag ccaacaaaca aatcgaaaaa ctgcaagaaa aactacagtt gacttataca
     6721 gaagaagctc cagagaatga ctcaacgact ctttctgaag caacagaaac aatagacaaa
     6781 caaattgata ctgaaaaaaa atggtggcaa ctttggaaat aactggaggg aacattcatg
     6841 acaagaaaaa atcttctatt cggttctatt ttactaggaa ttctaggatt cagtccgatg
     6901 ttattgtcag tgatcaataa agggaataaa catggtgaat tgaaattgaa tccaaattat
     6961 tacaacagtc ctaacgaaga attgtttgta aaagaaacag ataaataata aaaaagactg
     7021 ctctttcgag cagccagtta aaaaataatg agtgtttatt cttgaaaaag caacgcgctt
     7081 atgaagcgct accaaaaatt tatcatattt tattaaaaat aacaacccag taaaaaatga
     7141 ctggtaaaaa ttcacaaaga aacggaaaga agcaaccgca gaaaatcaag cttaaaaaga
     7201 gcttttgacc tagaccaatc gaaaatcaca aagtgagcgc ctgtatctat tgctacaggc
     7261 gctacttggc atagtgtaca ttatgtcaac aagaatttaa tataacatat tttcctaatg
     7321 cattctaact ataccacatt atcaaaaata taaattatct ttactaaata aaatattgac
     7381 aagcctatac cttttgtttt atgattttca caaagaaaac gttttctata tatttttctg
     7441 tatcaggagg aaattctatg aaaaaattta ctttaggggt agctttgtta actattgggg
     7501 cagttgtttt accgtcaact actatagttt atgcttcaga aaattcccaa gtggtatcat
     7561 taaacgaaaa caatgctact gaaaatgtag ttattgagta cactgaagat ggtatggtcg
     7621 taactaaata tgaggatggt tatagtgcgg aagattttgc aaattcttca gatatcatga
     7681 caagagctgc agttgcaaga tggggcaaaa aggaatatac aaatattgct gtaaccactg
     7741 gagtagctgc taatgctatt aatgctgcct tttgggcagg agggaaagct agcacttcta
     7801 tgtttggtat cccaggttgg gcaattggtg ctttgttaac aggtgcaagt tggactaaaa
     7861 aaggtgcttc tccagggaat gcagttgcaa aagaatggga caaaaatggt aatggctgga
     7921 ttggatttta ttaccaaagg ggatatgatg cagctggaag agttgtagct actagatatt
     7981 caacgttata agttaaggag tgatgttttt gatatctaaa tcattaaaat cattaaaaac
     8041 atttactaaa aagaatccgc aaatagctac tattcgtatt tttatatttg ctatagctgt
     8101 tgctataatt gttaatatgc taagcggagg gcaattatac aaatctggtt tatttttagt
     8161 gttaatctta gttgtcttag acataataaa gtct
//
LOCUS       DAZULY010000135         8033 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000135 DAZULY010000000
VERSION     DAZULY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.136"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            384..572
                     /locus_tag="ACG81A_001934"
     CDS             384..572
                     /locus_tag="ACG81A_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFS5015287.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            650..1663
                     /locus_tag="ACG81A_001935"
     CDS             650..1663
                     /locus_tag="ACG81A_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS5015288.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1730..2764)
                     /locus_tag="ACG81A_001936"
     CDS             complement(1730..2764)
                     /locus_tag="ACG81A_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5015289.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2879..5104
                     /locus_tag="ACG81A_001937"
     CDS             2879..5104
                     /locus_tag="ACG81A_001937"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS5015290.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5122..7005
                     /locus_tag="ACG81A_001938"
     CDS             5122..7005
                     /locus_tag="ACG81A_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFS5015291.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6986..7960
                     /locus_tag="ACG81A_001939"
     CDS             6986..7960
                     /locus_tag="ACG81A_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFS5015292.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2592 a         1465 c         1702 g         2274 t
ORIGIN      
        1 tagttagtct acattatttt ggcgatttgc caataaggac tgttgagttg tgcaaaatca
       61 aatctgggtc agaatgaacg tcattctgtc tcagattttt cgttatttca atttgattaa
      121 ctgattgatc aaaaagattt ttattttcat attctcaaat gattcaacac taaatataca
      181 tgtttgacgc ggaaattatt attcgatttt ggcggcatca ggggggatga cgacgtagca
      241 gacccattat tttactcaag aaaatgtccg tgcctattat ttagaatacg atagtgaacg
      301 tgctgggagt tttgttcttt tagagaaagt ctctgaagat aaattagttg tcttagattt
      361 tgactgtaac gaaaaatggc gaaatggaag agcaccagca agtgattgac cggatctacg
      421 aagcgacacg atatctgcct ttggatcgtt tgtgcttaag tcctcaatgt ggatttgctt
      481 caacagaaga agaaaatgtt ttgtttgaag aacaatggaa aaaatttctt ttgtcaaaag
      541 cattgcagaa gaagtttggg gaaaatcatt aaatcatcta ggagagcttg ataggaaata
      601 acactatggt ataatcagaa tgcttaatac taactactga gggagcctta tgaaaaagaa
      661 aattcttaaa tttatcggat ggttcttcgg aatcattctt gggatcgcgt ttgcaatctt
      721 tcttgccttc cagctttccc ccagacctgg agcattcttg atcaaccata tgttttcaaa
      781 tactgttcaa atcactgata aaaaaacgtt cgatcaagca gaaaaaaacg tggcgaaaaa
      841 aacagatttg acgtatgagt ccaagtataa agagaatact tatgacatct actatccaaa
      901 aaacagcaag gggcctgttc ctgttctatt ttgggtacat ggtggaggat tcgtcggcgg
      961 agataaatcg ggtgtcaaag aatttgcgac aaaattagtt tcagacgcca acatcgcagt
     1021 ggttgcgatg aattatgaat tagcgcctga ttcagaatac cctaatcaag ttcttcaagt
     1081 gaatgaactg atcaaagatt tattagctga agaaaaagac gacaaattgc tagacatgga
     1141 acaattattt tttggtggag atagtgcagg ttcacaaatc gcattgcaat atgcagctat
     1201 acaaacgaat gatgcgtatg ctaaacaaat gaaaatcaaa cagctacttc caaaagatag
     1261 tctgaaagga accatttctt attggggacc tgttgatttg aagctaacag cacagcagca
     1321 ttcggatgat cgatttatga agtttttcgt caaaaccgtt gcgtggtcgt tgattggaac
     1381 aaaagattgg aaaacgagtg agcaattgaa agaagctagt gtggctgacc atgtttctaa
     1441 ggaatttccg ccaacgtata taactgacgg aaatgcatac tcattccagg agcaaggtca
     1501 agcattagaa aatcggttga cagaattaaa ggtacccgtg caatccttgt tttatacaga
     1561 tacaaaaaaa gagattaccc atgaatacca atttgattac acgctaaaag aatcgcaaaa
     1621 ttgttaccag cagacattgg attttgtgaa taagtataag taagaggttg tgaaaaagcc
     1681 gtttcgtttt gagtatgaag aaacgaagcg gcttttcaca acctctcttt caataccttt
     1741 ttgttcttgg gataaaactt tccctaacaa tcaatcgtgc aggataagtc aaaatgattg
     1801 gttcgtcccg tataccgtca attcgctcca atgccattcg aacagctgct tgcccaattg
     1861 atttcgcatt gatttgaaca ctggtcaagc caggggagag gtttgcgatt gtctcgatat
     1921 tatcaaagct tgctacaaca atctcttctc caatttggat atttttttgt ttcaatgccc
     1981 gatagacacc aattgccatt ggatcgctcc cgacaagaat ggcagtagga agaggggatt
     2041 cattttctaa tagattagtc gcaagtacct caccttgttt ttctgtccag ccacctagat
     2101 gataagacat atatttttct aagtgatgat cgatcataaa atgctgatag gcacgcatcc
     2161 tcttttcatt tatagaaatc tgcttcccac cgaattcatc catatcgact ttatatcccc
     2221 cgatataggc tattctttta tgaccatttt ctaagaacaa ttccagtacg gttttagtca
     2281 tccgttcaag atctgcatac accatatcga cctcttgctg gatgtcaggg ttatccacga
     2341 cgactatttg catgttttgt tttaacaagg cggtaatcga ttctttcgta acagtcccaa
     2401 caacgatcac cgcacctaac tgatctagat cttcccattg tttaggatta tcagatagat
     2461 tatagattcg attcatccct aaatgcagac gtcgagcttc ttcttccatc cctactcgca
     2521 aatgtttgta atagatatcc tgttcttcgc tcgctttgcc gatcgtagag atcacaccaa
     2581 tcgaaggcat tctctttttg atatatttac gagtactgat gtcataatcc aacgcagcag
     2641 ctgcctctac tatccgttgc cgagtagtct gagccacatg gatcgtctga tcgttattca
     2701 acacacggga aactgtagca ggagagacat ttgcctttcg tgcgacatct ctaattccaa
     2761 ccatttttac acactcctca aaaaaataaa aaatttttat tgacaaaatt gtttagtaaa
     2821 cagtatacag gaattataaa aaaaagtaaa cggtttccgt tttgttggga gtgaaatgat
     2881 ggatattacg catagaaaaa cagataaaaa tttcatctta tttgatgaaa agagccgagt
     2941 cttccattta cgaaatacat ttctttctta tctgataaag atagaagaaa gcaatgtatt
     3001 ggcgcatatt tattttggca aaccagtcaa gcagtacaaa gacaataaaa attatccccg
     3061 aagagatcgt ggattttcag gaaatgttcc cttaaatcct gaccggtctc tgtcgaaaga
     3121 tacactaccg caagaatact cttctcatgg cagcatggac tttcgaacgc ccgcatcgat
     3181 cattcagcgc aagaatggat cagacttact ggatctgaga tatgacagcc attatattac
     3241 agatgggaag ccagagatcg aaggcttgcc tcaaacttat gtgatggaca agtcagaggc
     3301 acaaacgttg gtgatttcac ttaaggatag agaaacagcg atttatttcg atttgttcta
     3361 taccatattt actgatagag cagtgatcac acgatctgta aagataagaa atgaaaccgg
     3421 agaaacaatc aaactagaaa aagcggctag tttccagcta gactttgccc atactaggcg
     3481 atttgatgaa gtcattgctt tgccaggagc acatgtcaat gaaagacaga tttcacggca
     3541 atctgtttta tctggaacaa aagtatttga aagccgtcga ggaacgagca gtcaccatat
     3601 gaataacttt attgctttag ttcatcatca tacgacagaa aatactggag aagcgatcgg
     3661 acttcaattt gtttattccg gcaaccattc tttcgaactt gaaaaagatc agatcaatca
     3721 attgagagtc gtcggtggga tcaacagcca tcgtttttcg tgggaattga atgcaggcca
     3781 gtcttttcaa acacctgaaa tgatcctttc ttactcaagt cagggattga acaaaatgag
     3841 tcagatccac catgagttgc tacgtgaaag aatcgcaaga ggtcgtcatc aatttgctga
     3901 acggccgatt ctggtcaata actgggaagc cacttacttt gacttcaata gtgaaaaaat
     3961 caaggcgatc attgatgaag caaaggagct aggcatcgaa atgttcgtat tagatgatgg
     4021 ctggtttgga aaaagagatg cggacaattc ttcattagga gactggttcg agtatgaagg
     4081 caagttgaca aatggattac gtgaaatagc agattatgct catagcaaag gattgaaatt
     4141 tggtttatgg tttgaacctg aaatgatctc ggttgattct gaactgtatc gaacacatcc
     4201 tgattttctt atgcaggagc caggtagaat gccttctgct tccagatcac aacatgtact
     4261 ggattttaca aggttagatg ttcggcagac gattgagaaa caaatgagaa agattttaga
     4321 tacgattcct cttgactaca tcaaatggga tatgaaccgt tcgctttcag atgtctattc
     4381 gattaccctt gacccacaga gacaaggaga agtagcgcat cgttatatgc tcgggcttta
     4441 tgaactactt gaacatttga caactgatta tccggaaatc ttatgggaag gctgttctgg
     4501 tggaggtgga cggtttgatg ccgggtttat ttattatatg ccgcagtcat ggacaagtga
     4561 taacacggat gcagtggagc gtatgaaaat ccaatatgga acaagccttg cttatccgat
     4621 ttcgtccatc acagctcatg tatcagctgt accaaatcat cagaccgaca gatctacttc
     4681 gttgaaaaca agaggagaaa cagctatgag cgctgttttt ggttatgaac tggatttgac
     4741 aaagctgtct cctgaagaaa aaaagcaagt aaaagaccaa attatatcct atcaaacgat
     4801 tcgtccggtg atccagtatg gccattatta tcgtttggcg agtccttttg aagaaaatat
     4861 tgccgcatgg atgtttgtgt ctcctaaaca agatgaagct attgtgtttt tagggaggat
     4921 actagcttca gctcaaccag cgttccatga agtatatctg atggggttag atgatgaggc
     4981 actttatcag gaacagacct cgaagcggat attttcgggg gccgaattga tgacagttgg
     5041 actttacttc cccgattttc aaggtgattt ccaaacagaa ctgcttcatt tcaaaaagtt
     5101 atgagagaga aggaaaaaag tatgaaagca aatataaaat ggttagatga tccacaagtt
     5161 tttagagtga accaatcaaa tgcacatagc gatcatgttt gttatgcaac aagggaagaa
     5221 gtagaaacca catcctccct taaacagtct ttaaatggat catggcgttt ttgttattcg
     5281 ccaaatcctt cggtacgacc aaaaacattt tataaaaaag attttgatgc ctgttctttt
     5341 gatgaaatca ctgttcctag tcatattgaa ttagcaaatt atgacaagat ccattacatc
     5401 aatacgatgt atccgtggga gggacatgta tttcgccgtc cccctttcac gacaagccct
     5461 gagaaagagg cggaagggat cttttcagct gctaaagaca atccggttgg ttcgtatttg
     5521 acgacttttc gattagaaga aggtctaaag ggaaaacaaa tctcgattca ttttgctggt
     5581 gtagaacaag caatgtttat ttggttgaat ggtcatttta tcgggtatgc cgaagattcg
     5641 ttcactccgt ctgaatttga tttaacacct tatttggaag aagaaaatat tctagctgtg
     5701 gaggtttaca aaagaagtac agctgcattc ttggaagatc aggacttttt ccgatttttt
     5761 gggatcttca gagatgtctt cttagttgct cgaggacaaa accatttaga agatctttgg
     5821 atcaaacctc gcttagatga caaatgtcaa aatggcaaac ttgaagtaga tttgtctttt
     5881 gagcaagtag ccaaaaacag tcagctgaat gtagcgatac tagatcctga tgggaaggaa
     5941 atctatcaac agcaatatcc gttaaaagag caaatgacga ttcgcatcga tggatttgaa
     6001 aatgtggcac tgtgggggca ttttcagccc aatttatata agcttgaatt ggagacgata
     6061 gacgaagaaa aaagagtaac ggagtatatt gactattcct tcggttttcg taaaatcgaa
     6121 atcaaagata aaatcatcta cttcaatgga aaacggatga ttatcaacgg ggtaaatcga
     6181 catgagtggc atccgaaaaa aggacgcgcc atcactatag aagatatgga aaaagatttg
     6241 gctatcataa aaaatgcaaa gatcaacggc gtacggacat ctcattaccc caaccagatc
     6301 acttggtatt atttatgcga ccaagcagga atccatgtga tggcggaaac gaacctcgaa
     6361 tcccatggct catggcaaaa aatgggcgca tttgagcctt cttacaacgt accaggtgca
     6421 gtacctcagt ggcaggcggc tgttcttgat cgagcaaaaa cgaatttcga aacattaaaa
     6481 aaccatccaa gtattctttt ttggtctctt ggaaatgaat cttatgcgga agaaaacatt
     6541 cgacaaatga acgattactt caaaaaagta gaccccgatc gacttgtcca ttatgaaggg
     6601 aattttccaa atccagcatt cgaagattcg atttcagatg taaaaagctg catgtatgcg
     6661 ccaccagaag agatcattga ctaccttgaa aataaagcgg aaaaaccgtt catcttatgt
     6721 gaatatatgc atgacatggg gaattcgtta ggtggaatgg attcttacat gacgttgctt
     6781 gatcgttatg aaatgtacca aggaggctat atatgggatt ttaaagatca ggcgatccaa
     6841 gtgactgatc caatcacagg cagagaagtg atgcgctatg gcggtgactt tgacgataaa
     6901 ccaagtgact atgaattctc agggaatggg atcgtttttg cagatggaca agaaaaaccc
     6961 gccatgcagg aggtaagata ttattatgaa aaatacagta aataaaagtc atatggatac
     7021 ggaaaaagtt gcaatcgtct tcggcgactg tacattaggt gtcaaatcgg ggaatacgca
     7081 ttatattttt tcttatacaa gaggcggact ggaatcgctc aataaaaacg gaaaagaatg
     7141 gctataccgg gaaacacttc cagttttttg gcgagcaacg accgataatg acagaggtag
     7201 cggatttgga tttaaatccg gtatgtggtt aggagccgga ttgtatccaa aagtcaaaga
     7261 aatagaagta ctggtggaca ataaaaagat cgatttgccg attgctccag taaataatca
     7321 atattcaagt aatgaatacg ctgaaacggt tgaaatcatt tttacactgg aaatcaacac
     7381 agtaccaaaa acccaagtga caatttccta ccaaatcgct ggagagggta aaatgcttgt
     7441 caatatgcat tattatggac aaaaaggatt accggaatta cccgcattgg gtttaaggtt
     7501 cattatgcca acgaaagcaa aattttttga gtatgaaggc ctatcagggg aaacatatcc
     7561 ggatcggatg gctggtggtg tcgaaggtat ttataaagtg gcaggactcc ccgtttcccc
     7621 ataccttgtt ccccaagaat gcggcatgca tatggaaacg aaatgggtag aactaacaag
     7681 aaatcaaacg ttggataata cggataaaga tcaagaagat ttttcgttgc gatttgaaca
     7741 gcaggatacg ccatttgctt tttccgcttt accattcaaa gcagaagaac tggaacaggc
     7801 aacacatata gaagaactac cgccagaaag acgaacggtt cttacgattt ttgcaaaagt
     7861 aagaggagtc gggggtatca atagctgggg agcggatgtt gagtcacctt accgaatcag
     7921 cagcgaagag gaccatgaaa tcggctttat tattcagtag caatctttag attattattt
     7981 gaaaaacgga gtttacaaga caaagctgct ttattctata tgtcatgaac aag
//
LOCUS       DAZULY010000136         7953 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000136 DAZULY010000000
VERSION     DAZULY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.137"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1938
                     /locus_tag="ACG81A_001940"
     CDS             <1..1938
                     /locus_tag="ACG81A_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFS5015293.1"
                     /translation="SIEQLTQRWKKRIHWLFVHKSYFLVAAFFYCFTFVQIFVLEPKE
                     GWKETIQVALKIFTQRQAPMILTIIIIMGFFFLLLLISNRFWYALTATLIINLLLTIS
                     TVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVLLIVGGLILLLLLITSIIVQRRLQK
                     QYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSPSFLLFNLFKVNKTFFNQQGAVKDN
                     GPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYNTKADEINQTRSDWLNNQTIVLNLS
                     ESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLMLSVGYGGGTANMEWEGLTGLSISN
                     LSASLVTPYTQLVERQKISPNITDLFDEKIAIHPFTAALYRRKQVFDKFGFDRFYYLD
                     SPDKLTYTDKIEQSPRISDDSAYKETLKALQSNTETSQFIQLSTMQNHMPYDDYYSEL
                     NYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKFIQEIDKIDKPITFVFYGDHLPAIY
                     SGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNKEVVAPNNFGAMALEHANIKVTPYY
                     ALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTEDSQIIQEKNLTEKQKEILEDYRLI
                     QYDLVAGNQYSATWATQKIKD"
     gene            complement(1998..2714)
                     /locus_tag="ACG81A_001941"
     CDS             complement(1998..2714)
                     /locus_tag="ACG81A_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFS5015294.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(2941..3483)
                     /locus_tag="ACG81A_001942"
     CDS             complement(2941..3483)
                     /locus_tag="ACG81A_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFS5015295.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(3488..3715)
                     /locus_tag="ACG81A_001943"
     CDS             complement(3488..3715)
                     /locus_tag="ACG81A_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFS5015296.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(3794..5674)
                     /locus_tag="ACG81A_001944"
     CDS             complement(3794..5674)
                     /locus_tag="ACG81A_001944"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5015297.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(5863..6477)
                     /locus_tag="ACG81A_001945"
     CDS             complement(5863..6477)
                     /locus_tag="ACG81A_001945"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5015298.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            6619..7179
                     /locus_tag="ACG81A_001946"
     CDS             6619..7179
                     /locus_tag="ACG81A_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5015299.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(7484..7798)
                     /locus_tag="ACG81A_001947"
                     /pseudo
     CDS             complement(7484..7798)
                     /locus_tag="ACG81A_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT         2417 a         1587 c         1292 g         2657 t
ORIGIN      
        1 tcgattgaac aattaactca gcgttggaaa aaaagaattc attggctttt tgttcataaa
       61 agttattttt tagttgcagc atttttttac tgctttacct tcgtgcagat tttcgtttta
      121 gaaccaaaag aaggttggaa agaaactatc caggttgcct taaaaatatt tactcagcga
      181 caagcaccca tgatcctgac aatcatcatt attatgggat ttttcttctt actattgttg
      241 atttccaatc gtttttggta cgccttgact gctaccttga tcattaactt actgctcact
      301 atttcgacag taatcaaaat ggaaatgcgg gaagagcctg tctttccttc agatttaaaa
      361 atgctgaccg gattgtcaga actattatcg atggttagcc cagtcttatt aatcgtgggc
      421 ggattgatcc ttctcctatt attgattact agtattattg tacaacgtag actacaaaaa
      481 cagtattcct tgaagattca ttggaaacgt cgatttatca gtatcgctgt attattagga
      541 tgtttttcgg gtgtcttttt cattaatcac aaaaattcgc cttctttctt gctgtttaat
      601 ttatttaaag taaacaagac tttctttaac cagcaaggcg ccgtaaaaga taacggacca
      661 gtcattcaat tcctaaacaa tatcgacatt aaaatcatgg aagaacctgc tggctattcg
      721 aaagaaaaaa ttaatggaat aatgaaaaag tacaacacaa aggcagatga aatcaaccaa
      781 acaagaagtg actggttaaa caatcagacg atcgttctca atttaagtga aagcttcagt
      841 gatcctgcac gtgtccctaa tctgacagta cctacagacc ctattcctta catcaaaagc
      901 attataacag aaggaacagg aggattgatg ttatccgtcg gttatggtgg cggaacagct
      961 aacatggaat gggaaggact gacaggcttg agtatcagta acctgtctgc ttcgttagtt
     1021 acaccttata cccagctagt ggagcgtcag aaaataagtc caaatattac cgatctattc
     1081 gatgaaaaaa tagccatcca tccttttaca gctgctcttt atcgcagaaa acaagtgttt
     1141 gataaatttg gttttgacag gttctattat ttagacagtc cggataaatt aacttatacg
     1201 gataaaatag aacaaagccc acgcatatcg gatgattcag catacaaaga aaccttgaaa
     1261 gctttacaaa gcaatacgga aacgtcacaa ttcatccaat tatccactat gcaaaaccat
     1321 atgccttatg atgattacta tagtgaattg aactacactg ctgaaggaac tgctgtcatt
     1381 cctaatagaa gaaaagaatt acaaacttat atgcaaggcc ttcactatac cgatacagca
     1441 gtaaaaaaat tcatccaaga aatcgacaaa atcgataaac cgattacctt tgttttttat
     1501 ggtgaccatt tacccgcaat ctatagtggg aatgattcga aaaaatatgg attagaacag
     1561 caccagacag attatttcat ttataacaac aagtatagtc gtgaacaagc agctacgtta
     1621 aataaagaag ttgttgctcc taataacttt ggtgctatgg ctcttgagca tgcgaatatc
     1681 aaagtaactc cttactatgc gttgttgaca gaagtatcta atcatctacc tgcattaaca
     1741 attgatccta cagaaagctt gtctaatcgt tttaatggaa aacaggtctt tgttacagag
     1801 gatagtcaaa ttatccaaga aaaaaattta actgagaaac aaaaagaaat tctagaagat
     1861 tatcgtctga tacaatatga tcttgttgca ggtaaccagt acagtgcaac atgggcgaca
     1921 caaaaaataa aagactaaca aataaactga gcaaggggtt gtgataaaag acatgtcaca
     1981 accccttgtt tatctcatta atttgcttgt ccataaacga tttgattaaa gccatttttc
     2041 aattcattat tcgctgtctc gttgaagtaa agataattat cgggattccc ctgattttgt
     2101 tgtgtcacca atacttttgg ctgattttgc tgaaacccga atagatagac gtgctttttc
     2161 agatatggtt ctgtctctgc atcggaatag acggctgtga tttgatagtc tgcttgacct
     2221 gtcccacttt ctgaccattc tgcagaaaca ggcactccac caatagccat cttccactcc
     2281 ccgttcaaaa tagcttgagg gactttaaga ccatataagt ccacactcat ttgattcgta
     2341 taacttttgt attgttgtcc cattgtttgg ccccatttgg tcataaatgc ctctaattgt
     2401 gcggatttgt ctgcattcca cagtgtttgt tcaacttgtt cgctgctttc tgaagaggaa
     2461 gatgaagcct ctacagaaga acttgaagta gatgtttgag ttgtttcagt cgttttggaa
     2521 gactctgacg aagtagaggt atctgctgtt tctgtacttg ctgcagttga cttgctctct
     2581 gtgctagttg ttgtagtagt tgcaactgtt gctttcgctg tttcttttgt tgattctttt
     2641 ttcgattcat tcccgttggt acaaccagct aacaaaaaag ctagaccaat tgttactaaa
     2701 atcctttttt tcaattaaaa aactcctttc gattactccc acttctttat tctatcaaat
     2761 aagcaatgtg attttattga tttttcgtat tttctcatca atctttcaaa tcttctaaaa
     2821 actgagaagc acccgctgat gttgtctgta atacatcagc gggtgcttct tttattttaa
     2881 tcttcgtgat tttccaattt acctcacgct ttatatttgt atttgaagaa acatagactt
     2941 ttagtcatca tcgtcttcct catccaaatc ttgtcggaca gtttttcctt ggagtctttg
     3001 tacttggtag atgatttctt tattgaaact taaaagttca acaagcaatt gttgcaagcc
     3061 atatttttct tctttttcag ctaatttgat tgcccgtgtg ataaagacat tttggaaatc
     3121 aaaatctttt gcaaagtttt ccaacattgt tgaggctggt aaatatttgt tttgaccatg
     3181 ttcgtaaatt tttccataac gcatgacttc ttctaaagta gacggaacga tttcattttc
     3241 aatcactaac tcatgagaga ttttattgaa ccatctcgct tcttgatcag catattctga
     3301 aaaaagggta cttaattctt gcgaagatgt acccaaaaca tacaattgtg cttggatcaa
     3361 tttcatttct tctaatttaa aatttgctaa tacatgcccc accatggcgc cagcagttgg
     3421 tttatgatgg tcgatatctg cttgttttgt ttcttgaaca aatttttctt ccactatatt
     3481 cattgattta cgcctctttt acttttgctg attggacttc ataacctaat ttttccacaa
     3541 ctgcagctaa ttgttcaggg tggacttcat ctggatcaaa gtttgctttg attttgctcg
     3601 cattaaacaa aactttcgta ttgcttaccc caggctgttg agaaaccgca tgttcgattt
     3661 tttgcataca gcttggacaa gctagtgtat ctaattgcat aataattttt ttcatcgtag
     3721 ttcttccttc ctttttcttg atagtcttat tctagattac agcttccata aaaaaattga
     3781 ttcttatcaa gttttacgat ttttcttctt tgctttcgtg tccaaaatac aataaacgca
     3841 tcgcgttaaa gataacgatc aagatactag cttcgtgaac gaacatccca ctagccatgt
     3901 aaataaaccc ggcaaataag ccgttgagta aaaataagac gacaccaatg gcaatcacta
     3961 cattttcacg ggtatttaaa acagttttct tagccaagcg gtaggcatga accaacgatt
     4021 caaaattaga ttgcatcaag acaacgtcac tcgtttcgat agcaacatcc gttcctgatc
     4081 ccatagcaat cccgatatca gccgcagcca aagagggaga atcattgatt ccatctccaa
     4141 taaaagcaac gcgcaatcct tgttcttggt attttttcac aaattgaacc ttttcgtctg
     4201 gtaataattc agcatggact tcatcaatgc ctaacatatc agctacataa tcagcagtca
     4261 tctgattatc acctgtcagc atcactaact gttttgctcc tgcttgtttc aacttagcta
     4321 attgttcagc gacttctggt cgaatcacat ctgacacacc tatgatttga gtgatttttt
     4381 tgtcgattgc tacaataatg accgagctgc ctgtttgttg taattgatgt aaatcttgta
     4441 cttgctttgg tgtgagagaa agtttgtgag cggcaatcaa tttggtgttg cccgcacaaa
     4501 ctgtatgacc atcaatctca gctgtgattc cttgtccctt gattgtctga ttatctttga
     4561 ctgctaattg ttgaaaatca atgtctttct cctgagcata atggatcacg gcgcgcccca
     4621 gaggatgatc ggataacgtt tctaatcgag ccactaaagc taagctgtcc ccttcatcct
     4681 ctgtatagtt cttgatagtg gatacagcag tagttccttt ggtcaatgta ccagttttat
     4741 caaagacaaa agtatcgatt ttggaaaaag tatccattgc ttcaccgcct ttgactaaga
     4801 tgccccgttt cgctccatta ccgattcctg ctacattcga aacaggcgca ccaatgacta
     4861 aagcgccagg acaaccaaga accaaaacag tgattgctaa acgaaaatca cgactaatca
     4921 gaccaacaac caacgcaata agtaaaacgg ttggagtata atactgagaa aatcgatcaa
     4981 tgaatttttc cgcatgggat ttggtatctt gtgcttcttc tactaattcg ataattttgg
     5041 caaaggtcgt atcatctccg accttagttg cttcaatcgt cacgtagcca tctgctaaaa
     5101 tcgttccaga gaagacttca tctccactga ttttgacgac ttgtcgtgat tcaccagtca
     5161 ctgcggcttc atccgcatac cctttcccgt caatgactcg tccgtccaca ggaatagctc
     5221 caccagtttt tactaagacg atatcgcctt cttccacgtc atcgatttcc acttcttcag
     5281 tcgttccatc ttcttggaca acactagcag tagatggggc catctcggtc aaagccttga
     5341 ttgactcccg cgttttggtc aatgtttttt gttccagata gctaccaaac aaaaacaaga
     5401 atgtaacgat agctgactcc ttgtattctc cgatcataaa ggcaccaagc acagcaatag
     5461 taacgagtaa atcaattgag ataactttga ctttcactgc ctgatacgca tggatcataa
     5521 taggtatgac cgcaataata gaagctatgg ctagagcaat gttatagaac aagacaaaac
     5581 cagtaaattt tccgataaaa ccaaaaataa tcaacacagc tgataaggcg gtgatatgat
     5641 ttcgatgctc ttggatccac ttttgtaatt tcatctttct ccctcttttc ctttcattct
     5701 tacgagacta agtatagata cttatccatc ataaaaaatt gaccactatc aagtttagtc
     5761 aaaaaggcgg ttttcttttg gtagaataca ctacaaaaaa tagttagcaa gaagactcac
     5821 taaaagtgat cttgccaact attgtcgttt ctatcgaaat tttcattgaa tgattttcct
     5881 gtacagcata catcggagat ctatttcttt atctaagatt tttatcttgc taattttttc
     5941 ctttagtctt tcaaatccac gtcgttcata gaattgatac gtacagccac tatctgtaaa
     6001 aaggaatatt tcttttcctt tctctctttt ttcaaattct gttagcaact tggtaccgat
     6061 gccttttgtt ttcaaatcag gatttgcagc taaaaaaatg atctctccat ctggcgaatg
     6121 cttctttaaa tatgctgcaa gcatttgttc atttgctgtc tcgtaagctc ctttactttc
     6181 tttagcaaat atattttgta aaaaatcgaa tatcttaaca tatatttgct gtccaaaaga
     6241 acgatgtttc tttttttctc cttttatctc cgccagcaaa acacctgcca actcgtcctc
     6301 tgcatacaaa gcaagaattt gagttgctcg attcaactcc atatacaaaa aatatcttcc
     6361 gtaaagattc agcagaagtt cattttccat atatctctca aagttcattc cttcaacagc
     6421 aaaatagata gcttttttgt aatctttctt ttctaagggt ttaaaagtta gtttcatcat
     6481 ttcgcctcct caacttcaat gattgtgtta tacttacatt ataatagaca cagtgtctac
     6541 tatctataat caatcatcta taagtttgtc gattaagcga cagctttgct ttgatatgtc
     6601 ggataaggag ataggtaaat gaaaaagaca gatagacgcg tcaaaaaaac agaaaaagct
     6661 ttggctgaaa cactatccac attattagta aataaaaaga ttcaagcaat cactatacgc
     6721 gaactaacag aaacggcaga tgtacatcgt tcgacttttt atacacatta taaggatatc
     6781 tatgatctat atgatcaatt agaaagcaac ttttttcggg atctaaatga aattctctct
     6841 tatgatccag cccatagcta tgaggaacta tacactcgct tgattgacta tgtttacgca
     6901 aatacttttt tatatcgctt atttatcctc aaccatggtg atatttcgtt tcagaagaaa
     6961 gttaaacaag ttattgaaga aaactatctg aacatatggc tatatgaaga caagaaaacg
     7021 attatcacag aagaaatgag attctttact acgtaccacg tccaaggttg tctatccgtt
     7081 ctcaatcggt gggtagaaag caattacaag tactccaaaa aagaattatt agaactctta
     7141 cagaaactga atgatcacat agagaaaatc atgccttgaa taaaaacata ctaagcttag
     7201 ttaagaagaa ccgtaacctt ctcgtataac aactgaatct tatttagcca aattgtttac
     7261 tgaagatttt ttgagtaaaa tttctataat tcctacttat tgaaaagtag tttgtagtgc
     7321 tgcttctccc tctctaagtg atatatcttg ttgtaccaat caaattcacc tcatcattaa
     7381 taaattactt ttaggctaat ttattacaac ttataggttg aagctgtaac caatttatag
     7441 tcatattata tttttatcat ccagtttatt gaattcttct cgtttacctc aattcatttt
     7501 caaggggaat cagctcatca tctaacattt tacgccatgc tcgcttcatt tcttgtgggt
     7561 atttaaatgg tttcgcttct ggatgtttgg ctttatatcc ttcgtaatcc ttactgttga
     7621 taattgcttt catattagga attggatctt cacctggcat atattgcaga ttagaagcat
     7681 gtaatttttt ctcagaaaca aaccactcat tggaaaagac atgaatcaaa tcatcactta
     7741 cttgtgtaaa atagcgacgt ttaatgacaa agatatcttc attgctatct agtgtacctt
     7801 catttctttg tgcttaaatt caaagataat tttctcaata aagatttttg attccagtaa
     7861 gattccttta ctccttttac aacatagcgt tgtcctttgt tttgaaacat cttcttcccc
     7921 tctttgccat taaaaatagc ccaattctct ata
//
LOCUS       DAZULY010000137         7841 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000137 DAZULY010000000
VERSION     DAZULY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.138"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..850
                     /locus_tag="ACG81A_001948"
     CDS             143..850
                     /locus_tag="ACG81A_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015300.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQALP
                     ENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAI
                     AQQQAEQARQQAAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATL
                     SEAQARGLTPCSKCYGN"
     gene            complement(1012..2184)
                     /locus_tag="ACG81A_001949"
     CDS             complement(1012..2184)
                     /locus_tag="ACG81A_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFS5015301.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(2390..3205)
                     /locus_tag="ACG81A_001950"
     CDS             complement(2390..3205)
                     /locus_tag="ACG81A_001950"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFS5015302.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(3355..3801)
                     /locus_tag="ACG81A_001951"
     CDS             complement(3355..3801)
                     /locus_tag="ACG81A_001951"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFS5015303.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(3798..4313)
                     /locus_tag="ACG81A_001952"
     CDS             complement(3798..4313)
                     /locus_tag="ACG81A_001952"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFS5015304.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(4386..5300)
                     /locus_tag="ACG81A_001953"
                     /pseudo
     CDS             complement(4386..5300)
                     /locus_tag="ACG81A_001953"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(5297..7624)
                     /locus_tag="ACG81A_001954"
     CDS             complement(5297..7624)
                     /locus_tag="ACG81A_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFS5015305.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         2164 a         1543 c         1469 g         2665 t
ORIGIN      
        1 gcaaaaaatc ccgtgtatca acacgggatt agattaagtt tcctcaatca ctattcataa
       61 cacaattatt ataacaagta agtaaacaat tgaaaaggac atttattgaa aacggttgtt
      121 ttttacttaa ggaggaaatt tagtgaaaaa attagttgcg ttgtttttgg tcgcaggaag
      181 tttgttgagt gggtgtggtg ctactacgtc tactactcaa agcacaagtt cagctcaggc
      241 ttccagcagc agtaccacga tctccgaaag tacggcagat tcatcgaaag aacaaattga
      301 aaagaaaaaa gaagaagaga aacaaaaagc ggaaaaagct gcactagaaa aaaaagtgac
      361 agaagcggaa tctgctttgt cacaagctga agcaaatcct acaaaagaaa actatgcaac
      421 tgcttcagca gctatccaag cgctacctga aaacaaacaa gagttgacaa gccgtcttgc
      481 tgcaattgat tccacaatca aagcaaaaga agcagaagaa gaacggaaaa agcaagaaga
      541 aattgcacga caacaagcag aacaagcgca acaagaagca atcgcacagc aacaagcaga
      601 acaggcgagg caagaagcaa tcgcacagca acaagcagaa caggcgcgac agcaagcagc
      661 agctgaacag gcttctcagc aggcacaagc cgcagctcct gagcaaaata atgaacaaac
      721 tgtctatgtc acaccgaccg gttcaaaata ccatacacat aaatgtggaa atggtacgta
      781 tacccctgcc actttaagcg aagctcaagc tcgtggttta accccttgct ccaaatgtta
      841 tggaaactaa ttcaaaagtt atactataat aataaaattc ttctgaaaca taaaaaaata
      901 gaagctggcg ttccagatag taaaccaaaa atagcagttt acacgtccat ttcgtcagct
      961 tctctctttt ttcatttagt ctgttccctc tgcttttttg gacatcttca ttcattcttt
     1021 ctcaatcaaa tagtacacct ctttttcttt tcgcagttta tatcttcctt ttttcaattt
     1081 cattgaaaac tgtttcgaat gtttatgttt tacatggatt ttaagcagga gctcctcatg
     1141 ttgcaagcga tagttcctgc cttttaaatc agaaaatctc actagtccgt attcatcaga
     1201 agctttcaca aatggaactt tttctctagt aatatagtgt ttgccgttga gcgtttttac
     1261 agaaaagacc attccttcga ttggtcgtcc gtttgtgttc tttacataaa aagataaagg
     1321 atagtttcgc ttagcgatgg ccaaagaatc taaataagcg atgagtagcc aaactaagcc
     1381 gattgatcca agtagtatac cgataaacca aataaaaaat ttccattttt tccattgatc
     1441 aattccttgg atcgcttttt ttgcctcttt tgcttgataa ggaatacgat gccccgtcac
     1501 aagcagtcga tgtgtattga ccatataagg agtgcaggta agcaaagtca cgagatctct
     1561 gcctttttgg atacccaaag aatctacttc gtcaggtaag accacttgga ttttttctac
     1621 ttgatacgcc aatgtctttc cattgatctg gatgaaaaac tggtctcctt ttttcaactt
     1681 aggcaaatct gtaaataatt ttgcttctgg gagtccacga tgcccagaaa gaacggcatg
     1741 ggtgtttttc ccgccagtcg gataagaact tccttccaat aatgacgttc ctttttgcaa
     1801 aaatatttcc gttgtctgat cgaatatagg taagctgaca ttgatttttg ggatttcgat
     1861 gactccaagc atatgttcca taaaaaaggc attactaggt aaatctttta atgcttttgc
     1921 atcaactgct tggttgaaac tgcctatccc tggtgatacg ttttgctctg ccaactgttg
     1981 gtttttcttt gtcatttttt cctgctgttt ttttatttcc tctgaattct tattcgatgc
     2041 ttgcttttga taatggatga tcatttgctg ggcaagaaaa tcattcagtg aatcttctac
     2101 aaaaggataa gaaaagatcc cgatcccaac gactaaaatg attgccatga agatgtccag
     2161 tattcttatt ctgcttttat tcatcatgtc cacctatttt ctaatatgta tatgaagagg
     2221 ttgtgaaatc agcgccttga cctgagtatt agcgaagcaa acggcaaaat agttcctcct
     2281 attttgccgt ttgttgagcg aataccaaga gaccgaccac ttgtcacaac ctttctcccc
     2341 atttgaatca ctatcgttta tttatcgtat gatttttgtt tccaatagtt taagcttctg
     2401 tttcttgatg gcgttttttg aaatagatca ctgctcctcc aactaaagca gtacctacca
     2461 agatgaatac gatgatcccc ataccaccag ttgaaggcaa gattccttta ggttggttat
     2521 cgatcgttgt ttcatcagaa gttgttggat catcatctac tgtaaattca gtgtacgcag
     2581 cttcattctt aggtaatgcg tagccttctg gtgcttttgt ttcttgaagg cagtatgccc
     2641 cttgtttcag cccttcgatt gaaaatttcc cttctgaatc agaaatcaat ttcacttctt
     2701 cccagtcaga acctgtgact tcatctgacc aagcatatgg gtattctcct gaggcaaggt
     2761 ttgctatttt tttcattaat ttaacagttg gatcttttgc tcttagaata gaaaattctg
     2821 catcggctaa tttttcttgc gtgttcacat cttgtttgat gaagtggaaa tcaccgtagt
     2881 tttggacatt ttctacagta atcgtgttat ccgtagttgt attttcccga ttgactacac
     2941 cgtttggatt cccaagattg gcttcatctg cgccatggat gataccggtt gtttgttgca
     3001 attttccgtt ttttactata aaagtagttt ctttttgtgg attggttaat tgataaccat
     3061 ctggtgcttt tgtttcaact aaagtataat tgccgtctat taatccattg acactgatgg
     3121 atccttcagt atcagttgtg aatgtcaacg ctgactcagc aggatcatag agagcgacac
     3181 cagtgttttc atcaaaaccg gtcacgaatt gattctctga atttttgatc tggaattgcg
     3241 cccctgctaa cttatctacc cctgaaaagt tcccagcttt ctggattttg atattgcttt
     3301 ctttgatgac atttttagca tataggtgaa tcgtgtctaa aggtttgtca gggtctaggg
     3361 taagcttaac acgatatttg gcgacgtttc gacaccgggc tttggtgttt ctacaaagac
     3421 gtatacagca tctttcccat cagatttacc tggaagagtc aggttagcta atccttcacc
     3481 agaagtcgtt acacttgcga agggtgtagc gtctgctgga actgctgtgt ctttacttgg
     3541 atagtacgta gcagtgatgt catatacgtt gaattcaacg ccgttcaacg gttcgctatt
     3601 cggaaatgtc aattccgttc cgttgtttat ttgattttct ggtgctgaag tgaatttcaa
     3661 tttatgcaaa ttaaccgtaa cattttctgg tgtaggtgat acggctgcag aaacctctgc
     3721 agttcctagg ccgagaacga aaggaagcag catgatagca gttcctaaaa acgtccatat
     3781 ttttgttttg atgtttttca ttttatttcc ctcctattgt ttcctttttg ttgtttttta
     3841 taaaaccaag ctgtccctgt tgaaaggact agcaataacc ctaaaattga gaaacctcca
     3901 agcatctctt cgcttgtttt tggaaagctt cctccatttt tttcatttga tggaccattt
     3961 gtctgcatct gaactggcgt ttttccatta ttcgtcggat ttgcttttgg ttcgttgatg
     4021 atttccaatg gtataggatg aagcggatca taactgcctt tttcaacttc aaagagaatg
     4081 gttgattgac tcaatatata tcctttaggc ggtttaatct ccactagttc gtaacttcca
     4141 aaagacaatc catggacaga aaatgttcca tctttactgg aagtaaaaat cgttgctctt
     4201 gaatcatctt tggctaccca atgatttatc ccgttttctt gcgctaagta ttggtcattt
     4261 tttttgacta gaaattgagc atctgctagt ttttgtttcg tttgccgatc cactttgaca
     4321 aattgtttgc ctcctgtttg gatggcaaga tgtgtttcta ctgtttgatt gtcagtcgtc
     4381 aaacgtacat cgttatcaaa tgtcgttttt tcactgtttt ctttcaatgt catttggtaa
     4441 cggatcgtta atttttttcc agcaaacgaa ctaagatctt ttccagaatc tgataagaca
     4501 aaaccatgtt ttattttttg tgtgtgaaat gtatgaatct tgtctccacc aatcaaaaaa
     4561 tcgatcgttt ctggttctag ccataactga ggatcagctg tatctttgat cgtataggtt
     4621 cctatctcgt tgatgttctc aggaattgtt acggtgatga gataagggac tttatcccca
     4681 tctgcaaaat ctgtctgctt attctctact gtttttgtta atgtgagtgc agcaggtatt
     4741 ttttgttcgc tctttggata caagtggatg gtcgtcaatt tatgattaga tgaatctgtc
     4801 acaggaagca caacgacaaa tggtgctgat ttttctttta cttaatctga agtctttatt
     4861 tccgtaaatc ggtaaacagc gtcacgacct tgctcatctt tgtccgatgc actaaaagaa
     4921 gcaatgccct cttcctcgtc tactgttttt gtcacatttt ctgccagtat tttttcacta
     4981 tcactttttt gtgctagttt tctttgtgcg tcttccacac tacttccttc tgaccgcagc
     5041 ttgtaaaatt catccgagac atcatatata gcgaaagtaa cgccgttcaa cccttttact
     5101 ccttgaaagt tatccgggat atttattccg tcgttttgac tgatacttgg catcttatcg
     5161 ctttcgaaca ttcgattatg caaagtaaat gtaataggtg attctctagc tgacacagtc
     5221 ggagaaccta taaaaaagac cagtaggata aaaaagagaa cagcaaatat atccacacgt
     5281 acattttttg tttttttcat cgctgttcac ctgctttttt cttacgatgg ataaaataaa
     5341 agaagccaga aaaaataaag atcagtccta aaaagacaaa gggcagtaga cctatcccac
     5401 ctgtcgaagg aagcaagaga gtcggattga tcgtcaactt agcggtatct gttgcgataa
     5461 tcgtataatt attgtttaca cttccttcgc ctaaactaat cgttactatt tgtgatccaa
     5521 tatctgtccg gctgttaact gttagggtat aatccaatga ttcgcctaag acaacaccgg
     5581 taacgtggag atttccaacc gaaatcgagt gactttgggc atcataatta aaggcagcgt
     5641 catcgacagt aatcactgcg ttagctttat agatggtata agttcctgat gtgttggcta
     5701 tatcagtaaa tgacaacttg ggatttgctg ctttgacttt gtctattccg ctattcgtta
     5761 gtttaattgt atagctgcca gcattaacca tattttttgc tgattgtgca tctgctgtgt
     5821 aatccatact gctaagtgcc acttgtactg gagtaagcga actagtgaca ccctctgcta
     5881 gcttgacatg gattgacggg acacctgctt ttccatcata tgtcttactg ccactgagct
     5941 ctaccgcacc gctgatttgc ttaatcgtat agacatcaga tgtctgcacg atagtaaata
     6001 aatagtttgt tctggcagct agtttagcta gtccgctttt tgtcaattgc caagacgtat
     6061 aggttccaat ttcagtaggc gcagttactt caactccctt ttcattcatc gctttgaaat
     6121 cagttggatt ccatgtgatt tgatcaatac tgagatcatt gtttaaaagg gatgttgatt
     6181 ctgttttatc attaaaggca tatttaacga tcggtacaac agaaagacca tcatagtact
     6241 tactgccatt tgcattgatt gatactgttg catttgtctt ataatatagg atgacttcag
     6301 aaatctgttt tccatttgcg tcctttacga ttacatttcc atttgtatct aaactagcta
     6361 aaacactcat tgctgattgg ttctttgttt ctatcgctga aacagctgta aaggtatatc
     6421 cgttgatagt aggcgtatta gtcgtcaggt cttgtgcagc acttccgtaa gtaagattca
     6481 ttattttaga aggagcaatc atctgtccaa aatagtcttg atacttaata gtgattggtg
     6541 tttgtttgcc tgtataggtc accgtatcga cgttactttc tagggctgtg aaagaaccgt
     6601 ttgtttgtgc aaaagaatag cccgttactg ctgttggagt gataaccact ttgtcaccca
     6661 catggattcc agataatgta gtcccgtacg ttccgatttt tgctgctcca ccattgactg
     6721 tataagaaaa agttcctgtc cgattcgcat tgacctgaac cgttgctgct tgataggttg
     6781 gaaaatttga aacatttccc ttaccatcaa cgatctgccc atttttgatc gagtaccccg
     6841 tgaatggaat atcatttgct aactgtatac cgattgaaaa agtactcata cctcctccgg
     6901 caggaagccc tccccatgtt gagggtgtaa aagaataact tttatccgca ttgggtgtaa
     6961 ctgaatcgat cgtatttcca gcggtagcca cccccatata gatacttttt gggaatgtga
     7021 tagtagtacc atttactgtg gagccaataa acagattatg gatatctgga cggttatcaa
     7081 taaaagcgat tgctttggtt acgccttgta acgcagtttt atccgcactc atcgatccgt
     7141 taaccgcaac aatgactcca tctttggcat atatcactgt atatccatct ggtatggtat
     7201 gtccgttgaa attcgcagtg gccgcaacag tactaaatgt ctggcttcta cctgtttctg
     7261 aataagcttt ctcccctgct gcttttgctg ctgtgatttc tttttctgtc ggatcaatca
     7321 gttgagtcga ggatgtggtt gaggttgtct cgttgttgct agactgattg gtgctttcta
     7381 tggactgtat ttcagaatag ggtggactat ttgtctcctc tgtcgtttgg ttagcaaaat
     7441 ttgacgtttc tgtataaacg tttacataaa tacttgataa gctccctaag aaagtaaaaa
     7501 tcaagatgat cgaaatcata atgggatgta agtattttct tttagataca gctttgagtc
     7561 tagaaacaaa atgtttttga catatacgtt ggattttatt ttgagttttc ttaatcattt
     7621 tcatccctcc tctcaataaa aaatcatcaa ataatccttt acacgttatt ctaaatttaa
     7681 tttttattat tttattgatg tatagaaagt ttggggcttt ttttctcttt ttattctaag
     7741 gttattttag tcttgtcctt taatacaaag attttatttt taaataaaaa atactctttt
     7801 tttacgatta ttgaaaaaaa aagataaaac ttgattaatt g
//
LOCUS       DAZULY010000138         7799 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000138 DAZULY010000000
VERSION     DAZULY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.139"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(21..995)
                     /locus_tag="ACG81A_001955"
     CDS             complement(21..995)
                     /locus_tag="ACG81A_001955"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS5015306.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="ACG81A_001956"
     CDS             complement(1075..1950)
                     /locus_tag="ACG81A_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFS5015307.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="ACG81A_001957"
     CDS             2080..3987
                     /locus_tag="ACG81A_001957"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFS5015308.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..>7799)
                     /locus_tag="ACG81A_001958"
     CDS             complement(4528..>7799)
                     /locus_tag="ACG81A_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFS5015309.1"
                     /translation="KRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHVNIE
                     GMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSASRY
                     AIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYTNAQ
                     EGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMRLGL
                     GTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFPATN
                     SITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAARNM
                     TIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADGLLT
                     IKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEMTSY
                     ETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMAPGT
                     YSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLRGFD
                     ITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNCTSF
                     ENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIKNSV
                     AYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIVNSS
                     TSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSLNYY
                     WNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGNRQT
                     EKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGVAEN
                     AEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIFSAP
                     SKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPGNES
                     PNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMKQRK
                     K"
BASE COUNT         2194 a         1651 c         1455 g         2499 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgttttt
//
LOCUS       DAZULY010000139         7779 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000139 DAZULY010000000
VERSION     DAZULY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.140"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            46..747
                     /locus_tag="ACG81A_001959"
     CDS             46..747
                     /locus_tag="ACG81A_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFS5015310.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1356..2372)
                     /gene="tsaD"
                     /locus_tag="ACG81A_001960"
     CDS             complement(1356..2372)
                     /gene="tsaD"
                     /locus_tag="ACG81A_001960"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFS5015311.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2369..2833)
                     /gene="rimI"
                     /locus_tag="ACG81A_001961"
     CDS             complement(2369..2833)
                     /gene="rimI"
                     /locus_tag="ACG81A_001961"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS5015312.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2840..3382)
                     /gene="rimI"
                     /locus_tag="ACG81A_001962"
     CDS             complement(2840..3382)
                     /gene="rimI"
                     /locus_tag="ACG81A_001962"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS5015313.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3366..4088)
                     /gene="tsaB"
                     /locus_tag="ACG81A_001963"
     CDS             complement(3366..4088)
                     /gene="tsaB"
                     /locus_tag="ACG81A_001963"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFS5015314.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4279..5259)
                     /locus_tag="ACG81A_001964"
     CDS             complement(4279..5259)
                     /locus_tag="ACG81A_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5015315.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5283..6767)
                     /gene="galT"
                     /locus_tag="ACG81A_001965"
     CDS             complement(5283..6767)
                     /gene="galT"
                     /locus_tag="ACG81A_001965"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFS5015316.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6779..7768)
                     /gene="galE"
                     /locus_tag="ACG81A_001966"
     CDS             complement(6779..7768)
                     /gene="galE"
                     /locus_tag="ACG81A_001966"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFS5015317.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2172 a         1689 c         1384 g         2534 t
ORIGIN      
        1 aaaagtgaca ataaatatag tgaacaataa ggagaggact ttttaatgcg tgttaataaa
       61 atcgattttc acttttcaaa actcagatat caacctgatt ttgaacattt ctcggatgaa
      121 gagttccaac agatcaagga acatactgtt cttcgttctt ataaaaaagg acagatcctt
      181 ttcgacgatg gtgatcccag acaacggttg tatatcttga ccaaaggact cgttcgttta
      241 gaacgttatg atgaaagtgc aacttattac tactatgact atgtgaatac aaataacctt
      301 tttcctatgt caggaatgtt tggagaagag acctacactt atacagctca agcgatgact
      361 gatattgaaa cattctacct tcctgtttcc tactatgaaa aattagcacg aaaaaatccc
      421 gaacagttga tctatatgat acaaaaaatt tctaaagtag ttgaaataca tgaattgcgt
      481 atccaaatcg gattgacctc tagcgccttt gaccgggtaa aacaaaattt attcattttg
      541 aaagaagaat tgggcgtacc aacaaaagat ggtcatatca ccattcctta tccgatcacc
      601 ttaaaagaat tagcagtgaa tagcggcacg acacgtgaaa ctgctggaca agtaatcaaa
      661 caactacgtg attccgggct gttagattat gacaaaaagc gttttacttt ctgtaagaca
      721 gctactactc cagctatgag agaataacgc gaacaaaaat aagcagtgac cctgataaat
      781 atcagggtca ctgcttattt tcctccctaa aacttctgaa agatccggca gttttagatc
      841 actggacaag actcaaagat tttctatgtg tgtttcagtt atgtaatatt cactgtttgc
      901 aaaaaatact gttagattaa ctagatgaag cgtcttgtcc tcatccgatc tagattattc
      961 gatattcctc gacaataaac gattactcag tgagggcaat cagaggaacg gataatgata
     1021 aataagctgt aaaaaatagc aaaataacaa taatgattaa aagtatgtaa aaaatagggc
     1081 tttgacaaaa acgtgtcaca gccctatttc gattaaacgg tgtgtacctg tcaactcttc
     1141 ggtcacaagt cataatgttc ataaacatga ggagctgttt tttgaaattg ttccttgtga
     1201 atcagagtta aacgacaggc ttacgaactc ttgattaaac ggcgttcaaa agcttgcata
     1261 cgcggtaata agaacagcca ggtcaaaaac atgagaagcc gtttttcctg ggctgttctt
     1321 attacttgat gcagggcagc tttttcacaa cctctttatt cagataaaac caagcttgga
     1381 tcagcattca actgatagct agcaaaattt cttttttgca tttccacatg tgctgctgct
     1441 ccaatcattg cagcattatc tccacaaagt cgaagagggg gaatcactag ttctacctca
     1501 gggagtttag ctgccaaagc atcctgcaat ccttcacgca acccttgatt ggccgctaca
     1561 cctccagcta taatcagttg cttgactgga taattttgac aagctcgaag tgttttagta
     1621 atcaatacat cgatcacact tgcttgaaag ctagctgcta aatcattttt gtctagatct
     1681 tctccacgct gttgtgcatt atgcaccaaa ttgataaatg cactttttaa tccgctaaaa
     1741 ctgaaatcat aattatcttc atggatcatc gctcgcggaa aatggtaatt atcttttccc
     1801 tgatgagcta gctgatcgat ttcttttccg cttgggtaag aaagacctaa tactcttcca
     1861 actttgtcat aagcttctcc tgctgcgtca tctcttgtct ccccaatgat ttcatatgat
     1921 ccatcttctt gcatatagac taattctgta tgtcctccgc taacaagaag tgccatcaat
     1981 ggaaactgga atggtttaac cagacgggct gcatagatat gccctgccat atggtttaca
     2041 ggaataagcg gcaattggtg tgcccaagca aaagccttag ctgcagaaat accaatcagc
     2101 aacgagccga caagtcctgg tccataagtg acagcgactg ccgataaatc ttctgccgac
     2161 acaccagctt ctaccagtgc atcttccaag cataaggtga tttgttctac gtgatggcgg
     2221 ctagctactt caggtactac gccaccaaaa cgtttatgac tgtttatttg agaagcaacg
     2281 atgttagata agatctctgt tccatttcta acgaccgcta cactcgtttc atcacaactt
     2341 gattcaatcg ccagaatcaa ttcttcattc atttcatcgt ctccgttttt aactttctgc
     2401 tcataatgat cgcatcttcc gtcggattgc gataatatga tttccgttta cctaatatac
     2461 gaaacttttc tgattcataa agttttcttg ctgcttcatt agatagacgt acttctaaaa
     2521 aaacatgcag cgaatctttt atttgttccg taatcagtaa ttgtaacaaa tgccgtgcat
     2581 gcccttttcc ttgttctgaa actgcaacag caatatttgt gatctcgact tcatctatta
     2641 ctttcgagta tccaataaag ccacagatct ggttatcttc agctaaaagc agataatcag
     2701 agtgaggctg ttggatgtct gccaaaaact gcattttcgt ccaaggtgaa ccatgtggat
     2761 aagcattttc gctaatcatc catacgcatt ctgccaagta cgcttcatca aaatcttttt
     2821 ttctgcaaat caactaatct cactcatttt ctaaaagttt tatcatatcc aacgcatctt
     2881 cttttgtttc agtgtaatat ctttttttga tcgttgttgc ttgaaacccc aatttccgat
     2941 aaagtctttg tgcatcttga ttactcatac gcacttctaa tgacatcgtc tcacagcgat
     3001 tcattatagc aaagttctcg atttcgtcaa taagcagcga accgattcgt ttacgctgat
     3061 atgccggtcg aacggcaata ttcgtgatat ggcaatctaa accgaacgct cttgaaccga
     3121 taaaaccaat cattcttcca ttatcaaaga tacctagata tagatgagga agcggtgact
     3181 tcaattctga tataaaggca ctttttgtcc atggcaattc gccattatag acttctcgtt
     3241 cgacatccag cagctctttg atatcttctg tagtcaattc tcttagtacg taacgacgat
     3301 cgatttgttc gactgttttt tctgtataaa tcattttatc ttgaaaaaag cttctggcat
     3361 acgctctaaa ttttttcaac ataactttcg tctccaggtg tgtgcgtctc caaccatttt
     3421 tcttctgctt ctactcgttt taaatagcga ggaagaaaag cttgtttctc tgcaactggt
     3481 tcaactaaag cacctaattg agccacagtt acaccacttg gaatatccca tgctggaact
     3541 gtattaatca tcgcactagg caatatttcc tttatttcag agaagaattt gtgacaatca
     3601 ctcccgacga aataaatagc ttcattcagc ttttgtagtt gtgtgaacaa atcagaagca
     3661 gaaatatgta catcttcaag tacagtttct aatctatttt ctttccagcg gtatgcccca
     3721 gcgtatacat tttttcttcg agcatcaaac atgggcacaa taatattatc cacaccgatg
     3781 caattagccg cgatggtttt cagactagat actcctacta gctctttatc taatgtataa
     3841 gcaatcgttt ttgctgtcgt tacacctata cgaagccctg tataagaacc gggaccatca
     3901 gaaaccgcga ttcggtcaat gtcttttgga cttatttgaa gatctgcaaa caattggtcg
     3961 attgcaggca tcagtgttac actgtggttg cgtttgatat ttgtttgtat ttgtccaaga
     4021 atttgttcgt cttctaccac accgatcgcc aaagtttggt ttgcagtatc tattcctaat
     4081 gtaatcatat atttgatcct ttctgctatg acattctcgc cttccattgt agcatacttt
     4141 tttattctga ggatgaaaat accaaaggca aaactagtac aataatacat gattatctgc
     4201 tggcgggcaa attttgcccc ttcctgaaac acaaaaaagc tgcggtatca ctccattttc
     4261 ccacagcttt tccatctttt atttcgttga ttctcgaata atcaactctg tttccacaat
     4321 aatctttctt gaaaccggtg ctttggaatt acttaattga agaatcgtct ccactgctag
     4381 ctctcccatc cattcggtat aaacttttac agtagaaagc gagggcgtta catatttcgc
     4441 tacactgaca tcattgaagc ccatcacaga aatatcttct ggtaccacta aacctttttc
     4501 ttgaatcgct ttcagtgcgc caatcgctaa tgcatcatta gctgcaaaaa gcgcatcagg
     4561 aacatgcgga ttcgttttta aatattgctt cactgcttga tatcctgact cgacagaaaa
     4621 atctgcttcg ataattggac cttcgtcgta gcctttttct gccaaaactt gttgaaatgc
     4681 cataattctg ggatctttta gccgctgatg gtttttcttc gtatattctg ttccagcaag
     4741 catagcgata tgttgcgctt tatgggagac aaagtgttca atgacttttt gaacacttga
     4801 cttaaagtcg acaactagag agtcgattcc ttgagaagtg ccgtcagaat ctaccaaaag
     4861 caaggcattt tgggatcctt tcatttctaa taactgagct tcgtcgaatt ttcctagtgc
     4921 gatcgttcca tctgccggaa gatcactaat ggaatcccac gtttctttca ataaagtaac
     4981 gtttgcttct tctgcctttt tttcgattcc taggcgaata gataaataat aaagatcttc
     5041 aagctcttcc gtatcgtcgt accactggat caggcgaagc gtttgcttag tatttgctgc
     5101 atttttacga tgtttcgtat aatttaagcg ttctgctgct tcaaaaattt tttgctttgt
     5161 ggctaagcca acggaaagat caggatcata gttgagaact cttgaaacag tagcaggaga
     5221 aactcctgcc aactgcgcaa tgtctttaat tgttgccatt tgtctctcct ccttttttac
     5281 gtttataaaa tcgcagtaaa tcgttcaaac gctgcctgtc ctttttcatc tcgcttgaat
     5341 actccagcgt cttccagtac acgtgcaaaa acctgaccta cagatttttg tacgacatcg
     5401 gtgaccgtat ctttcgtcaa atcaggatgt tcttttttta attgttctgc ccacggacga
     5461 tgatattcag ccatctggtt ttcttctcct aataagaaat gctctacttc tactagttct
     5521 ggtttcaaac gaggtggtaa aacagcaagc cccattactt cgatcaaccc aatgttctcc
     5581 tttttgatat gttggacatc tttatgtgga tgaaaaatcc catcaggatg ttcttctgaa
     5641 acattgttgt ctcgaaggac aaggtccaac tcaaagtaat tgtcccttct tcgtgcaatt
     5701 ggcgtaatcg tatggtgtgg tgtaccatct gctgaaaaag ctctgataga tactgtttca
     5761 tccgaatatg ctttccattt ttccaatact gagttagctg cttcaataag caagtttttc
     5821 tctgttgatt gcaaacggat cacagacatc ggccatttca cgactcctgc gatgacttgc
     5881 ggataatctt ctagtttgat aagtttttct atcggtgctt ttgccatagg aaattcatga
     5941 cgtcctgcct gataatgatc atgagaaaga atcgatcctc caacaattgg taagtcagca
     6001 ttcgaaccaa caaagtaatg aggcaagact tcagtaatcc gaagcagtct agcaaatgtt
     6061 tcctttgtga ttttcatcgg gcggtgttgt tctgataaaa tgatcgaatg ttcattgtaa
     6121 taagcataag gggaatattg gaatccccag ctttcaccgt ctagattcat tcgaataatc
     6181 cggtgattgg tgcgggctgg atagttcaag cgacctctat atccttcgtt ttccatgcaa
     6241 agcatgcagg taggatagtc tacttttacc gcctcgcgtt ctgctgcgat ttgttttgga
     6301 tctttttctg gcttagataa attgattgtg atttccagtt caccatactc cgactctgcc
     6361 ggaaatttga tgttttttgc gatggctctt gttttgatgt agtcattttt cttacttaaa
     6421 ttaaagaaat actcagttgc ttcctctggg cttttcgaat agtgctgtgc aaagaatgca
     6481 ttgacgactg aaggtggcgg tgtgagaaaa tccattaatt gcgcttcaaa aatctcttgt
     6541 tctgctggca attctgaaag aacaccattt tctcttgcct tagcgacaag ctgatccaat
     6601 aagtctacag attcttcctt aactggtctt acttcaatgc tttctagagc atcttctcct
     6661 accatcccta aaagacgatt ctgtaaatat aagcggtcca tttccatcca tccgccagct
     6721 tcgatcgcaa gtgtggcaaa atcacaaatc gtttgactga ttgacatatc tcattcccct
     6781 attcttcata accatttgga tggctgacat gccaatccca tgctgttttg ataatcgctt
     6841 tgatatctgt gtattcaggt ttccagccca gaatctctct cgctttttca cttgaagcga
     6901 ctaatcggct aggatctcca gctcttcttg gtgctatttc tgctggaatt tcttttcctg
     6961 tcacttctct agctgcttct agcatttcct tgactgagta tcctttgctg ctgcctaaat
     7021 tgaagaagtt gctttcattt ccttctttca agtattccaa agctaaaata tgtgcagcaa
     7081 tcaaatcttc tacttgaaca taatctctga tacatgttcc atctggtgta tcataatcgt
     7141 ctccataaac agccaaggct tttctttgac caagagctac ttgtaaaatg attgggacaa
     7201 gatgcgtttc tggtgtatga tcttctccga tagatgcatc tgcttttgct ccggctacat
     7261 tgaagtagcg caaagcgaca tagcgcatgc catatgcttg gtcacaccac ttcatcattt
     7321 tttccatcat caatttactt tcaccatacg gatttttagg attagctggt gtatcttctg
     7381 tgattggaga ctctttcggc tctccataag ttgctgcagt agaagagaat acgattttat
     7441 tcacgttgaa ctcgtgcatg acttcaagta agatttgcat accgtatacg ttgttgttga
     7501 aatacatcaa tggtttttcg acagattctc ctactaaaga gcttgcagca aaatggatta
     7561 ctccttcaat cggttccttt tcaaatacgg aacgtagaaa ttctttatcc cggatgtctc
     7621 cttcataaaa atgagcatct ggatgtactg cttgtttgtg acctgtgagc aaattatcta
     7681 ccacagctac ttgatacccc ttttgaacta gctggtctac agcgtgcgaa ccgatataac
     7741 cggctccgcc tagtactaaa attgtcataa aaaacactc
//
LOCUS       DAZULY010000140         7585 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000140 DAZULY010000000
VERSION     DAZULY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.141"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(59..787)
                     /locus_tag="ACG81A_001967"
     CDS             complement(59..787)
                     /locus_tag="ACG81A_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFS5015318.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(800..2080)
                     /locus_tag="ACG81A_001968"
     CDS             complement(800..2080)
                     /locus_tag="ACG81A_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS5015319.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2221..2823)
                     /locus_tag="ACG81A_001969"
     CDS             complement(2221..2823)
                     /locus_tag="ACG81A_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFS5015320.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3068..3274
                     /locus_tag="ACG81A_001970"
     CDS             3068..3274
                     /locus_tag="ACG81A_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015321.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3586..4305
                     /locus_tag="ACG81A_001971"
     CDS             3586..4305
                     /locus_tag="ACG81A_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS5015322.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4386..5069)
                     /locus_tag="ACG81A_001972"
     CDS             complement(4386..5069)
                     /locus_tag="ACG81A_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFS5015323.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5287..7473
                     /locus_tag="ACG81A_001973"
     CDS             5287..7473
                     /locus_tag="ACG81A_001973"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFS5015324.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2717 a         1280 c         1425 g         2163 t
ORIGIN      
        1 agaaccgaag ggtttggatc ggctggtcat ttagtcggtc caaactattc ggttcttttt
       61 atgaaatcaa tttctttaat ccacggatag aggctgggat ggtctcgccg tttgccaacg
      121 tgacttctct ggttttttta ttaatcgacc gcacattttg tgtattgata acataggatt
      181 tatgacaacg gataaaattc cgatgcattt tctcgatttc tttgatcttc tcatagaatt
      241 gtaattgggc atgagtcaaa tggacttcca gtttatgtgg cgctcctagc gaagtagaaa
      301 aatattggat gtctttgatc ggtaaaaatt tgatctcatc attaaactct attttgaaga
      361 tatctttttg gatttgggac atttgaaaat cttcgaaaat tttgctcagg atatgctcga
      421 cattttcttt taaatcataa agatcttctt tgacgatata atcagtcggt tcgatgttgc
      481 tttttaagat tttcatcgac atttccgtat gggaagtcac atagattatt tttcctaaag
      541 gatctaattc acggatcaat ttaccgatcg ctacaccatc catttcactg tcttctagtt
      601 cgatatctaa aaagtaaata ccaaaagaag aagttctgat cttggctgca ttgagaagtt
      661 cttccggatc agaggtagaa atatggacat aactgtctaa gttttcaatc atgattctat
      721 ttttgatcac cgttgataaa acttctaatt gttttggatc gtcttcacaa atataaatgg
      781 gaatcatatc attactcctt tattttattt gtatataaat cgtttgagaa aaacgctggt
      841 tttcaattga tgtttcaagg aaaagataag gattggtctt tgtcaattca tccaagataa
      901 acaagccaag gccttcgttt tttggatttg tttttgttga gaaccctttt tgtttcaact
      961 ggtttaacgg taatgtattt gttgttgtat tgatcacacg aatcatcacg gaatgattct
     1021 tcttgagtat ggtcaattcg atttttttct tctttgcagc actagctcct tcaatcgcat
     1081 tgtccaagat aataccaagc atacgtacaa ggtttaccgt atgtttcgta tccagttcga
     1141 ttggctcagg aaaatgaagc tggacgatca accctttttc ctgtgccatc atgagtttta
     1201 atgacagtaa actacggatc tcaggaactt tcatattatc caacatactt agattggccg
     1261 gaaaaaggtt catcatctgg cgtgtaggta atatcgtttg atcataataa gaccgtaatc
     1321 cttctaagtc gccttctgaa atatagctgt gaagagaaaa aagcagattt tggtaatcat
     1381 gtttgaattc tctcaagtct tgatagtttt tttctagatc tttgatataa tcctgttgca
     1441 tcctgacttg ttgctctaat aactgatcag ctaatttctt attagagatt ttccaataat
     1501 aaaccaagat gatcaggcca acacacagat atagaatgaa aaaactcgta ttcaaagcta
     1561 agacatcagg tgtttcacca gaaaaacggg tataaaaaat aatgatatag tatgtaaata
     1621 atatcaatgt cccgatcgta ccgatcatac ggcggtccac agtgtgaaac caagtctcta
     1681 acgatttttt taaacaaaaa gctaataaag cagataaaaa agcggataag acaatgtgaa
     1741 aatactgcaa tgaatctcct ggttcaataa ccgaagattt caaaacaaaa aaatcgtcga
     1801 tgcttgttaa gtgatctcct aaaagcgacg tcaataaccc aaaaggaagc gcgtaaacca
     1861 attgttgacg cgaagtcgaa aagaagaaaa aagtaagcat cagcattcct atagcaatta
     1921 gagcagtaaa aatccctaaa atcgaataga cccatccaat caaaatacaa gaaatcagaa
     1981 tgactcccgc aaacagatat ttgtatcgga tgtgatggtc aatcaataag atacaaattg
     2041 tgtaaatcaa atattgcaac gctatggacc ataaaaacat aaagtacagc tcccattgat
     2101 ttttaccaaa ttatagcaca attagcgtat tattttttta atttttcagg gattttgaat
     2161 tcagaactca taccaaagca gcagccagat acaaaaaagt cagataagtt agttaattgt
     2221 ttaatgattg tttttttcat gaaagattcc tccaaaattc atcaagtagt ttgtaatagg
     2281 aagcaacgta gctatttcta aaacaatccc aagtagcaaa taggtagaaa agtaagaagc
     2341 tgagaaaaat aaagtaatga gagaaaaaat tagaaacatg ctgatcgcct tctttaataa
     2401 tgggggacgt ttctcgggtt caatgggatt gattgctgta cctcttgatc catagcgaag
     2461 cagtaacaaa aagcagctga ttaacaaaaa gaccatcatt gggaaggaga tagtcaattg
     2521 cagatgtttc atcaccaagg gaacgcccca taaataaagt agtgtccaca gcagacaaac
     2581 gaattcagag tcggaatgca atccataagc atatctgcgg atgcagatat aagaaagaaa
     2641 ggtgattcct gtttccgccc atgagcctgt caccagagaa aagactagaa cgaccgctag
     2701 ttttccaaga ttattcaata aaacttctaa ggcaaatttt acttggacat acaccatatc
     2761 ttcttccgat ttgtctccgc tgaataaggc ttctaataat ttgttcacaa ctttatcttt
     2821 catctcatac gtacctccac cacaaatttt atgctaaaaa aaataaaaaa cgtcttttgt
     2881 ttcataaaaa gtggttttga catcaaaacc gtcatgatct cctaaaaaaa tcttcgtttt
     2941 ccaagaacct taccaaaaaa agtgacagtt ttgatacgaa aagtactttt tatgacaagt
     3001 ttgtgctttt tcgagtgatt tatgttaaaa aattactgac cttataaaag gtgatggagg
     3061 gattcttatg gaaaatgaac aattgtttga agtcggcgaa acatatgcat ttaaatctga
     3121 tttatttgaa tcaccaataa aaggaaaaat agaaaaaatc tatgaaaata gtgtgatgat
     3181 acgtgtcatg aaatgcgcag tagaagacaa tgataccgct cataatctgc actggcagtt
     3241 agttgtacgc aaatcaagta caatagaaga ataaaaacag ataaaatgtt gagagcataa
     3301 agaggttgtg agtctcccgt ttagctctga gttacaagga ataatgttaa aaaacagctt
     3361 ttcatgtttt atgaatattg ccttgtgacc gaggtgttgg caggagaata ctgtttattt
     3421 gaaaaaaggg ctgcgacaag acttttgtca cagcctttat tttcatgaat aggagttact
     3481 tttggctact tttagagaag tagcttttaa ggagcagata aagtcaaggc ttttatttct
     3541 ataaatgaag tagtaaaatg aagaacagat tttattacag gaaggatgcc gataattgtg
     3601 atcattgttg caggaacgat cggtgcaggg aaaagcacac taactgaaat gcttgcccaa
     3661 gatttagaaa caaaaccttt ttatgagaac gtggaagata atgaagtatt gccgctattt
     3721 tattctaatc cagaaaagta tactttttta ttacagattt tctttttgaa caaacgcttt
     3781 ttggcaatca aagatgcttt cagccatgat gacaacgtac tagaccgctc catctacgaa
     3841 gatagtatgc tgtttcattt gaatgcagat ttagggcgtg tttctgaagt agaagtaaag
     3901 cagtatgaag gtcttttaga aacgatgctg aaggagttag aagaaatttc gccacaaaag
     3961 aaaccagatc tactggttta tattcgtgta tcctttgaaa ctatgttagc gagaatcaaa
     4021 aaaagaggcc gggaatatga acagctagaa caagatcctg aactctattc ctactataaa
     4081 gaactcaatc gacgatacga agagtggtat gaacagtttg atatatgtcc caaaatagtg
     4141 atcgatgggg acaaatatga tttcgtagcc gatccggcat gtggagaaca aatcgtacaa
     4201 gaaatcaaga tgcgggcaaa gaaaatgaca gaagaggcag atgcccagtc gatccgctac
     4261 actacaaata acccgaaagc aaagggaatc tttacaaata aataatataa aaactaagca
     4321 aattgctcac tcgcagatat ttctctgtga gcagcatctt gcttagtttt tttgattcat
     4381 tgagatcaaa tagtgaaccg attcgtatga taaaattgcc ataaatgaaa tgtctgagtg
     4441 agggccatga tcaaaaataa aatggaaacg atcaaaatcg aatgggcgcc aaacagatga
     4501 tgacagaaaa caaggacaat agctcctgcg ataaaggcaa gaacgacact tagatagtgg
     4561 aaaaatcgat caaaatctgc tttttcttta gaaatacaat aatcatataa gttcgttgct
     4621 acccctttta aatttccagt tgtaaataaa ttagtgtagg cacgcccgtt gattttatca
     4681 aaagctaccc actggatcga tgcagtaaaa gcaatcaaaa tcgttactaa tgcgggatgc
     4741 tcatggacgg gtgtcaaact aacaaagaaa aaaatcactg cttcataata tagaacaaac
     4801 aagcgccaga aatgtttttc tttttttcta aaataatcaa ttagatactt tgttagaaga
     4861 atccctaaga aaaagaaaag ggtagacaat acttttttac caacacttag ccattctccg
     4921 tcaaatgctt ggatgatcga tagaatgata ttgccagttt gagcagaggc aaaagcacca
     4981 tattggatat aagtataact gtccatggct cctccgataa atgtcagcaa aagtcctatt
     5041 gtacgcccct cgtgaaagga agtctgcatt tttttcatca ccttttatga atatttttgt
     5101 cttccttatc ttagcataaa cgattgaaaa aaaggaaaaa ttttcagaaa actaaaatca
     5161 tatcgcttcc cattttcatt tttagaaagg tctgttagta taaagaaaaa atagacaacg
     5221 aaactttaaa taaccaactt gaaataaaca aattcaaaga agaaagatgg gaaggaaatc
     5281 aatcagttga atgaaaaaga aattctccga ttacaaaaag aattgaaacg ctgggagaac
     5341 aagtataaaa agcttcaaca agtctttcgg ctacaagaag aaatcattgc tacctatgaa
     5401 gcggcttatg gcgaagcacc cggacttatt gaggaagaag tacaagtaaa agaaagtgaa
     5461 ggaaacagct ctttctttaa aatgatggag aaacgttcct ataatcgctt caatcaagaa
     5521 caaaaagcag ctatcacata tgatatgaag aaacatcttc gaatcatagc aggagctggg
     5581 agcggcaaaa cgcaaacaat ctgtgcaaaa gctgtttatc tgatgacgca aaaacaagta
     5641 gacgaagaac gcatactaat gatcactttt acaaggaacg ctgcaaatga actaaaaaaa
     5701 cgagtagata atttcagcca gagaaaaacc gctgtccata tcggaacatt ccatagtatc
     5761 ttttttagga tatacaatga aatttgtcga aaatttcctg aagtagcaat gcaaggaata
     5821 caaggagact tttcaaagga tactgcacaa aaagtgaatg ctgttttgca acagctgata
     5881 cgtaaatata atctttatat atttgatcaa tatggagaaa aaacgattgc ttctcggtta
     5941 gactattggc agaatatgaa cctttcagta gaagaaatgg ttcagttagt taaagaaaaa
     6001 tatgattcca ttgataagaa tgcacgacag ccgatatcag aacgactgta tggattgctc
     6061 acggaactcc aagaacaaaa aagaagccag caattgctag agttcaatga tgtactccaa
     6121 aatctgaaaa gcgctttgga gaatgaggag atccagcatt atatcggcca aaaatatgat
     6181 tatctattca ttgatgaatt tcaagatact aatccacttc aatgggaaat cgtgaaacgg
     6241 atgacaaagg aaaacaggat caagttgatt gttgttggag atgatgatca gagtatctat
     6301 ggattcagag gatcagagcc agcatacatc aagaactttg aaaaagaata tcctaccaaa
     6361 actcttttct tattgactaa ttatcgttct cgggcagcta ttgtccaagg agcaaataga
     6421 ttgatcagct ataataaaaa tgatcggata ccaaaggcga tgattccagc acaaaaagaa
     6481 gcaggtgtta tggaagccta tttgttttct gatactaaag cagaaagtca gtggctaatc
     6541 aaacaaattc agacatttat tcaaaaaaat gggaaatata aggaatcaat catattatac
     6601 cgatcagctt cacaaacgca gcagcttgtc caatctctgc tgaaaacgaa tattcctttt
     6661 gtactggaag cagatagtcc atatgaaggt atctttggta tcaagtcttt ccaacatttc
     6721 tatcaaaaaa ttgttcattg gcaaacagca ctgaatctgc aaaagaaaaa tcaggcctac
     6781 cagcaattgc tccggcagct gatggcagac tgctacctga aaaaaagtga atgcgatcaa
     6841 tacttttctc ctaaatatca aaatatagcc gttgcagatt atattttgtc gataagcccc
     6901 aatttaaaat cgaaatctgc agaaatccga gagtttgagg aagcgttgcg taaaacagac
     6961 cagcatcaag aaatctattc gcttgttcaa agctatcttc atctgccacg tatagctaag
     7021 gaaatcgacc agagtgatca agaatggata cgagaagaag taaagcagca tgctactttc
     7081 agaagactcc aagccttatc gcaagaaacc caagcgacag ctaaagaatt aaaaacccgc
     7141 ttgattgctt acagacaagg aaaacttgat gcactatgta tccagagcat tcacaaaagt
     7201 aaaggtctta gttaccgaaa tgtctttttg attggttgta acgaaggaag tttgccttat
     7261 aacggagcga cagaaaagaa agtaatcgac tttaatgaaa taaaagcaga accagtgacg
     7321 acgatagaag aagaacgaag actattttat gttgcgatga ctagagcaag aaatcgtctg
     7381 tatctatgtg tacctcaaat gaaaaattca agaaaattga aagtatcgag atttatcaaa
     7441 gaaaccggga gtcgtgtgaa aaaaggaaag tgatagaaga gagggtgtga cagaagtggt
     7501 cagtttcaag aaagaagaag gaatgtacga aaattgcttc tcaaattttt gtgaaatttc
     7561 gacttatttt tcgaagaagc tactt
//
LOCUS       DAZULY010000141         7568 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000141 DAZULY010000000
VERSION     DAZULY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.142"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(77..280)
                     /locus_tag="ACG81A_001974"
                     /pseudo
     CDS             complement(77..280)
                     /locus_tag="ACG81A_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            246..443
                     /locus_tag="ACG81A_001975"
     CDS             246..443
                     /locus_tag="ACG81A_001975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015325.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(486..1061)
                     /locus_tag="ACG81A_001976"
     CDS             complement(486..1061)
                     /locus_tag="ACG81A_001976"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFS5015326.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1216..1742)
                     /locus_tag="ACG81A_001977"
                     /pseudo
     CDS             complement(1216..1742)
                     /locus_tag="ACG81A_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2199..3710
                     /gene="gadC"
                     /locus_tag="ACG81A_001978"
     CDS             2199..3710
                     /gene="gadC"
                     /locus_tag="ACG81A_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFS5015327.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            3723..5123
                     /locus_tag="ACG81A_001979"
     CDS             3723..5123
                     /locus_tag="ACG81A_001979"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFS5015328.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5288..6728)
                     /locus_tag="ACG81A_001980"
                     /pseudo
     CDS             complement(5288..6728)
                     /locus_tag="ACG81A_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            7121..7568
                     /locus_tag="ACG81A_001981"
                     /pseudo
     CDS             7121..7568
                     /locus_tag="ACG81A_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
BASE COUNT         2371 a         1282 c         1376 g         2539 t
ORIGIN      
        1 tcaatctttt taccactcca aacagggagc gtcaataatt ttgtgtaaat aactcgtcct
       61 tctgcaaaat aatgggttac tcagtaaaca ttgaagctaa tgtatccgtc acttgttgga
      121 agcctttatg gcttctattt aagaattttt gattgtatgt atcaaagatg gatactagga
      181 aacgctctag tgattcttca ttttgaaact gctcttttct gcggctgtat ttcttgattt
      241 gtttattgaa agattcgatt gtagaaccag aaggaatgga gaacactttt gaactttaca
      301 acaaaactac tgttgataaa ttaattccaa ttttagttcc aactgattta aatggtcatg
      361 atgttttact tcgttttaac ggatttttag gtgaggatta tagataccta aaaatttctg
      421 atcctcaacc tttaacagaa taataaaaaa cgtgtaatca attatttgat tacacgtttt
      481 ttatttcaat cttcgttgta gtagtggttg cgacataccg aaaggtaatc ttcattacct
      541 cctactacaa tctgttcacc tgaataaact ggaataccat caacaatacg agcattcatt
      601 acagcctttt tattgcaaaa atggcaaata gttttaattt cctcaatttt atcagcaaat
      661 gtcaataatg cttcggatcc cttgaagaga ttattttgaa aatcattctt caatccatag
      721 caaattactg gtacttcctc attcattacg ataagtgata gatcaataat attctctcta
      781 cttaaaaatt gagactcatc gaccaaaata caatcaactt ttccgtactg atcaataatc
      841 tccactaatt ctccgtacac atccatttca gaagtaattg caatagctgg tttcgaaaca
      901 cctatacgac tctcaacttt tccaaatcct gatcgagtat ctattgcgct tgtcattatg
      961 ataactctct tcccttgttc ttcataatta tgagccactt tcagtagctc aatactcttt
     1021 ccactattca ttgttccata tctgtaaaat aattgtgcca ttaaatttat tcccttcttt
     1081 tacaattcaa ttattcctta attccatctg cttttatgca gctgctcgtt tattccttga
     1141 tatgtctata acatctacta tccctaaaac caatttataa gccattagag atggtattcc
     1201 tgaacatctc tcatattgtt ttttgagtgg tatatcatct gaaaaactga aggaatcatg
     1261 aaaatagcga catacttatc atttagagtt ctaactttaa atgcatttac ttcttcagtc
     1321 agcactttag tcatgttgga catggtttgg ggcgtgtagt gatgcccata cattttctca
     1381 atcaagtctg cgatttccga catggtaata ccttttcgga agaggtgaat gacggtctcc
     1441 tctaatgtgt cattcgtccg tctataagca ggaacagtct gttgcttgaa ctcgccattg
     1501 cggtcgcgcg gaatctggag atgaagttcc ccatactcgg tcttgaccgt acggtcatag
     1561 gagccgttac gagaattacc cgtgttaaaa ccaatgcgat cgtacttttc gtaatcgagg
     1621 aaagccgtta actccgtttt gagaagcgta ttgacagctt tttccaaatg gacacggaat
     1681 aatccattca aatcgccttt gttcgctaga gtctttagga tttctgtagt aaaatcgttc
     1741 atatggaagt cctcttttct gtgaatgtgt tgtggtaact ctattctaca gaagggactt
     1801 ccttttttct atttacacaa aatattttac actctcaaca aaagtatctt cgattaatac
     1861 ttgctcaagt tatgtgcagc tacgaaaaag taggtaccga ggtactttct ttttcgcagc
     1921 tttttttgga cttatgagaa gtacattcgc tggaagaata cgatataaaa cgagcttttt
     1981 cactaaagat attaatagag tgaaagaaac aagcttttgt tgtcagtttt ttcacttttc
     2041 taaaaatgaa aataaaaaat tctggaagtg gagaagaatt ttgcttgcta tactttgtct
     2101 tgtagaaaag aggtagacaa aataatgcaa gctcaaataa gcgattttcc ataaccttgg
     2161 taggaatgac aaattaaata ggaaagggaa gtaataatat gaataaaaaa aagttatctc
     2221 tattcggttt ttttgccctg acagcttcca tggttttgac tgtttatgag tatccgacct
     2281 ttgcaacatc acaattacat ttggttttct ttcttctttt gggaggtatt ttatggtttc
     2341 taccagttgc tttatgtgct gcagaaatgg caacagttga aggatggaaa gatggtggga
     2401 ttttcagttg ggttagtcaa actttaggag aacgttttgg ttttgcagct atttttttcc
     2461 aatggtttca aattacagta ggttttatta caatgatcta cttcatacta ggagcgctgt
     2521 cgtatgtaat taattttcca gagcttaata cagatccatt tatcaagttt attggtgtat
     2581 tagtcatttt ctgggtactg actttttcac agttgggcgg gaccaatcgt accgctaaaa
     2641 ttgctaaagc aggatttgtc attggaattg ttattccttc gatcattctc tttggtttag
     2701 cagcagccta ctttatcggc ggtaatccga ttcaaatggc tattaatgcg aaagctttta
     2761 ttcctgactt ttctcagctt tcaactttag tagtatttgt atcttttata ttggcttata
     2821 tgggcgtaga agcttcagca tcacatatta atgagctgga aaatccccaa agaaactacc
     2881 cattggcaat gtttttacta gtgattttgg ccatcgcctt agatgcaatc ggcggtttct
     2941 cagtagcagc ggttattcca caacatgact tgtctctcag tgctggtgta atccaaacat
     3001 ttgaaacgct tattttacat tttaacagtc atttacattg gttggtaaaa gtgattgctc
     3061 tcatgattgc ctgtggcgtt atgggtgaag ttagttcttg ggtagttggt ccatcacgag
     3121 ggatgtttgc agctgctcag cgaggtctgt taccagtttt cttccggaaa actaataaac
     3181 aagacgttcc agttccgttg attatgatac aaggaattat cgttactatt tgggcagcag
     3241 tcttaacttt tggtggagga ggcaacaatt tatcattctt agtagcaatc tccttaactg
     3301 ttgtcattta tctcgtagga tatttactat ttttcctagg ttactttgta ttagttttta
     3361 agaaaaaaga cttaaaacga acttacaatg taccaggaaa aactgttggc aaaacgatta
     3421 tcggtggaat tggttttatt ttatcagtgt tcgcattgct tatctctttt gtaccaccag
     3481 cttcaattgc agctagtgag caccgaacat accaaatcat actacttatt agctttgtag
     3541 tgactgttct tttacctttt atcatttatg aagtacatga taaaagtgct cacacaacta
     3601 ttgaggaacc tactcacgtg aaagctgaag acgttaatcc cgctgtttat ccagctgcta
     3661 gaggagagca ccacgttaca aaaaatgatg aacatatttt aaaacattag gaggaattca
     3721 ttatgttata cggaaaagat aatcaagaag aaaaaaacta tttggaacca atttttggct
     3781 ctgcaagtga ggatgttgac ttgcctaaat ataagttaaa caaagaatcc attgaaccac
     3841 gaattgctta tcaattagta caagacgaga tgttggatga aggaaatgcg cgattaaact
     3901 tagctacttt ttgtcaaacg tatatggaac ctgaagcagt gaaattgatg acccaaacgt
     3961 tagaaaaaaa tgcaattgat aaatcagaat acccacgaac aacggaaatt gaaaaccgct
     4021 gtgtaaatat gattgctgat ttatggcatg ctccaaataa tgaaaaattc atgggaactt
     4081 caacgatcgg ctcttcagaa gcctgcatgc tgggtggtat ggccatgaaa tttgcttggc
     4141 gtaaacgtgc tgaaaaatta ggtcttgata ttcaagcaaa aaaacctaac ctggtgatct
     4201 cttctggtta ccaagtttgt tgggaaaaat tctgtgtata ttgggatgtg gaactgagag
     4261 aagtcccaat ggatgaaaaa catatgtcaa ttaatctaga tactgtcatg gattatgtgg
     4321 atgagtacac aattggtatt gtaggtatta tgggtattac ttacactggt cgttatgatg
     4381 atatcaaggg tctgaatgat ttagttgaag ctcacaataa acaaactgac tataaagtat
     4441 acattcatgt tgacgctgca tcgggtggct tttatgcacc atttactgaa cctgatctag
     4501 tttgggattt tcaattgaaa aatgttatct caattaattc ttcaggtcac aaatatggtt
     4561 tggtatatcc aggtgtgggt tgggtcttat ggcgtgacca acaatactta ccagaagaat
     4621 tagtatttaa agttagttac ttaggtggag aaatgccaac tatggctatc aacttctctc
     4681 atagtgcagc acaactaatt gggcaatact acaactttgt acgctatggc tttgatggtt
     4741 atcgtgatat tcaccaaaga actcatgatg ttgctgttta tttagccaaa gagatcgaaa
     4801 aaactggtat ttttgaaatc attaatgatg gatcagaatt gccagttgtg tgctataagc
     4861 tgaaagaaga tcccaatcgc gaatggacac tatatgattt atctgatcgt ctgttaatga
     4921 agggatggca agtcccagcc tacccattac ctaaagactt ggatcaatta attattcaac
     4981 gcttagttgt tcgagcagac tttggaatga acatggctgg tgattatgta caagatatga
     5041 accaagcaat tgaagagttg aataaagctc atattgttta tcataaaaaa caggatgtga
     5101 aaacgtacgg ctttactcac taaaattctt cagagaaata taaaagaagt taattaacta
     5161 ataaaaggag ttgtgattac cacacagctc cttaaatcta tttgaataaa tatatgatat
     5221 tcatttgtta cactctcaat cttagctgtt gttctattca tcattactct tattgtaagt
     5281 agttcagtca aatttgcttg ttcaattgat cgataacagt atgaattgtt tgaaaagtaa
     5341 tcggatatat aattgttttc tcactaccta agccttcttg gtataaagaa ctatcgcaaa
     5401 gtataatccg ctgagtacaa aagaaagtgt cgtctactat ttctctaagt tgtttacaaa
     5461 tgatattact aatagaaaag tgttctccca aaatgtcttc taactgttgt cgatagtata
     5521 aatacctaaa gtctgacgga gctactatga acacttcaat tggagatttc cgtttatgct
     5581 catttaaaag acttaaacta atggttagtt tacgaataga attctcattc caacgcagaa
     5641 ataaattgtt ttgttttcgc cattgttcaa gaatttcatg aacttctaag tacaaagcgc
     5701 gttgctcttc atttaacaag taagtcttat caggttgaaa tatctgaata tcagcccaaa
     5761 gtgagcggat aaacgggaga agtgctttat caaaaagtat ctgattttct tcagatatat
     5821 tcagagataa gataagtttt tgactgagct caatcaccaa agggttatct gctacatggt
     5881 tttgataaac aacgttcaag tctttttgta acaattcaaa gttatcgcat agataatttc
     5941 tagagttgaa tagaaagtag atataaaggc attcatcttc gtcatgacat aaagagaaag
     6001 aaagttgctc taatcctttc tgtattagct gaaacaaggg agtatgtcgg gcaactcttt
     6061 tttcttcctg actaaaatat attgggttag tcttagatct ctgcaaaccg atagcgatac
     6121 ctaataaaat aaattgtttt tcaactgtag ggtaacttct tgaaaaaaaa tgtttttcta
     6181 cataatcaat caattgttga ccgctttgaa taattgattt atttagctgg atcaactctg
     6241 tccgatttgt ctcatagatt atcgttagta acaaacttcg ataatccttt tcaggaattt
     6301 caaaatggcc atcagcattt aagtactgca tttgtttaaa aaaattaatc aactcatttc
     6361 gtaagttgta aactttgctt aacgaaatga aggtagtatc agctatatta ctccaattgt
     6421 gttcccctga taaataatgt cctagtacat tgaaaaagtc agagcgctga tagatgagtt
     6481 gagtgagtgc caacctcgaa aacgaagagt cgaatgcgac tgtaaagcta cttctagatt
     6541 tctccactga aataatttgc tgatggagta tgtcggtaat gcattccaag tcgttagtga
     6601 cagacagtga gtgggcaaac gttcaattga tctgctattt cgcgaatctg aatggtccct
     6661 ttttcgtgta attgctcaca aatgtaaact tgacgaaaaa tacttttctc cagataggtt
     6721 tccaacatag tactcctcct agtcatttta tacataacta ttataactct accaatcgga
     6781 atagtcctgt aatatattga atcaaaaata taaattataa aataattaga tggggtattt
     6841 ggtttaagta tcccttatat agtaataaat ttataggtgt gattctcgaa ttaatacccg
     6901 aaaatgtgag agaattttta tggatttttt aggatacaaa ttaattattt ttgcttttat
     6961 cctgattacc agtccagtac tgtcggtatc ccattttgca gcaaaatcat gttgataaag
     7021 tgacaagaag actgatgaga tatattatcc aaattgagaa gataattaat cggattcttg
     7081 agtataccct actttttaag aaagtagggt atacaagcgt atgaataaag acataacttt
     7141 ccctgtgatg gacgttcggc ttgttccaat taccaaaata caggcgaacg attataatcc
     7201 caatcgagta gcgtctccgg aaatgaactt attggagctt tctatcattg aagatggctt
     7261 tactcaacca ttagtttgtt attacaagga aaaagaagag aagtatatct tggttgatgt
     7321 gcttcatagg tatcgtgttg ctaaggaaag actcaactta aaagttgtac cggtaacagt
     7381 tattgataaa cccatataag aacgtatggc ttcaacaatt cgacacaatc gtgcaagagg
     7441 gacccatgaa ataagaggaa tggaaattgt catccaaaga ttagtaaaaa atggatggtc
     7501 agacaagaga atatccaaag aattaggcat ggacattgaa gaaatttttc gctttaagca
     7561 agtgtctg
//
LOCUS       DAZULY010000142         7423 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000142 DAZULY010000000
VERSION     DAZULY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.143"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            142..1533
                     /locus_tag="ACG81A_001982"
     CDS             142..1533
                     /locus_tag="ACG81A_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5015329.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(1606..2253)
                     /locus_tag="ACG81A_001983"
     CDS             complement(1606..2253)
                     /locus_tag="ACG81A_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5015330.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(2261..2947)
                     /locus_tag="ACG81A_001984"
     CDS             complement(2261..2947)
                     /locus_tag="ACG81A_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5015331.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2953..4026)
                     /locus_tag="ACG81A_001985"
     CDS             complement(2953..4026)
                     /locus_tag="ACG81A_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5015332.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            4248..4922
                     /locus_tag="ACG81A_001986"
     CDS             4248..4922
                     /locus_tag="ACG81A_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5015333.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4927..6267
                     /locus_tag="ACG81A_001987"
     CDS             4927..6267
                     /locus_tag="ACG81A_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFS5015334.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(6416..7402)
                     /locus_tag="ACG81A_001988"
     CDS             complement(6416..7402)
                     /locus_tag="ACG81A_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFS5015335.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         2303 a         1382 c         1308 g         2430 t
ORIGIN      
        1 ctaaagggaa tcacaccaat tttcatacca attggtttca tttatgaagt tgattccttc
       61 gattaatgag cgtttcattg aaagaaaagg gttgagggag aaaaacatat gatcaggttg
      121 ttggaaggaa actatagaaa gatggaaaaa atcattcgaa ttctgtccat ttctgacaag
      181 tggatgacta aaaaagaact tgccaaacga attggtagtt cagaatctac atttgtaagg
      241 tatatcgaag agatcaaaat ccgctgggga acggatgtaa cgattcaatc ttcacaaaaa
      301 ttggggtatc gtcttgaaca tttgaatgta ccagcttatt taaatgttct gatggaaatg
      361 tcgcaaagtt ctacaaacag tcgtttatta aatgagatta ttgtcaatcc gggtaaaacg
      421 attgagtatt attgtgaaaa aatttccatc agccggtctt cttttacacg taagttgaaa
      481 caatgcaatc aaattttaga aagctattta ttaaaagtga tcgttgacca aggttttcag
      541 ttaactagcc ttaaaagtga gctccagttg cgtatctttg ttaccttttt ctttttaagt
      601 tactacggaa gacgcacgtt gccatttcca atcgaaaaaa agaagatcaa agaaattatg
      661 gtcaagaata attgtcagat gaatttgttt caggaagaaa gcccatatga gcaatctttc
      721 tttattatgt attttgttgt gcatctcatg agagaatccc aaggaactcc atttgaatgg
      781 aaagctggag actcttatgt acagttgata aatatcgata aaaaagactt cttaatcttg
      841 aaaaatctct taggaaaatt gaagtacagc agctatataa aagtagtgaa ctattttgtc
      901 aatacgattt tttcagaatt ttcggagaaa gaaaaaaatg aaatcagtga gcagatacga
      961 aaaaatatgt cctactgttc gttttcatcc gctgattgca aagaaaaaaa cgaagaatat
     1021 gttgttcaat tattaacgca aatttatttt ttctcccgta ttttcccata cgatacgact
     1081 catttgacga tccgtgcaac ttactttgca cagcagatgc aacaccgcca gatacgtcta
     1141 tatgctggat ttcgtgcaga acttcaattt tattccactc tactgaatca gaatctgatt
     1201 tattatttat ctagctatat cttttggatg cttatcggta atcctgaaat tcaccattat
     1261 acttctgata aaaaaatact gattatcagc gacttaagtc ttcgacatag tcaatatatt
     1321 gaagaatata tcagtgatat tcttgctgtg cataaaattc actctgagac aactgctatt
     1381 actgaagatc agctgtcgaa atacaatctt ctcgagtatg atctgatcgt tacgaatcaa
     1441 cccattttga atgcccatgt tcctcatata ttgattgatg atagtgtgtc attcgcaaat
     1501 gaagaagaac tcatccgctt attcgagtta tgaataatca aaaaacagct atgtataaac
     1561 caccctgata aaaatttcag gacaaataca catagctgtt tctttttatt gtattccttc
     1621 agcaatccaa cgagatttat agcgaaagtc ttttaacaac tcttcttttg tccaattatt
     1681 tgcaatgaga tccgtcaaag tgttcattac gagggtcatg gaaaagtata ggaaactaac
     1741 tgcttcttcc gacgattgaa tggagaaatg attgattctt aataattcca gccatttttc
     1801 gttcattgga gaatcagccg atagttctct acgtttggtt gtctgctgat catttcgctg
     1861 ggttaacaag tataaccttt gccaatagat actttcgtga tctaagtcgc agcaccactc
     1921 taaatatttt tcgatcccca aaaagaaatg ttgtttgttt tcgctaatga attttaagat
     1981 atcttgatgg actatgtgag aactgctttt gatcgtatac aaataagcat cttccaaatc
     2041 ttgaaaatat ttataaaaag caccgcgcga catccccatc ttgtcaacga tttctgagac
     2101 tttgacttgg ctgatgtgct gtgagcaaaa tatctccagt aataggtcat cgattctctg
     2161 tttctttgcg ttcgataaat tgaaaaaagt ttcttttggc atccacatct ctcctttatc
     2221 tctgattata cgtggatagc ccttcattgt catgaaggat tcattccttc tttttctctt
     2281 cttctgtcaa gtacccatct tccattcgat aaacgcgatc actccattga atcatacgga
     2341 catcatgagt aaccataata gtcgcttttt gtttctcatg agcttcttta actagtaatt
     2401 tgacgacttc atatgcatga tctgtatcca aactagctgt tggttcgtct gctaagatca
     2461 agcttggctc attatataaa gcgcgagcaa ttgccactct ctgtctttct ccaccagaaa
     2521 ggtctttagg ataactattt ttaagtgact ggatatctaa agattgcagc aaggaatcga
     2581 tttttgttga atcttctttt tttgaagtaa cttggtcaac tagttcaaat tgttccttta
     2641 ccgttaaaaa aggaatcaaa ttagagcttt ggagaataaa tcctatttct ttaaagcgta
     2701 aatctgctcg ttttttttct ggaagctcag ttagtgagtg accgttgatc actatttctc
     2761 cggaagtcgg tgtttgcaat cctcctgaaa tggtaaggaa agtactttta cctgaaccag
     2821 aaggaccaat gatgctgacg aattctcctt cctttacata aaaatcaatt gctttcaaag
     2881 ctgagacctt tgtatgacca gaaccaaatg ttttggtaac ttttttcatc gttaatattt
     2941 ttttcatttt tattaacctc ctattgctgt aattgggtct actttggtta tcgtacgtat
     3001 cgaaaacaat ccgcctagca ttgcgacaat gatcaaaatc ccactatata gcaaccattg
     3061 tgaccaaaaa acagcaaaag gcatcgcgct tggtaaaatc agactagtca aataagcaaa
     3121 caaaagagca agcagaacgc ctaccacccc aacaataaag gactgtgcta ctaaagattt
     3181 agcgatgaag aaatttttga tcccttgtgc tttcattact ccaaaaatag cagtcttctg
     3241 aagtgtcatc acgtacatga aaataccgac cacggcagca accactaaga acaaaaagta
     3301 tatcatcgca tttaaggtca tattttgggc agaatagcca ggaatacttt caatgaatgt
     3361 cccaatagtt aataactgta gacccttcga tgctttttct ttcgaaattt tagctgaatc
     3421 ctttattacg atcccattga tcggctgttc ttcttcagaa gcaaacggct gttgtccata
     3481 cttcaaagca gtccacgtat ctagggacgc atagatgacg ggagaaactg tgtaataagt
     3541 ttcaggaaag ataccgacaa tcgtcaaagg atcgtcgtaa ctgccaattt tgatcgtatc
     3601 tccaatttta tagcctgaat cagccagatt ttgagagatg atgatttcat tctttgtttt
     3661 aaattcatgc cctttcgtga gttttggtaa taaaaatgct tctttcgttg tgccgaaaac
     3721 tgtaacattt tctttgtttt ttcctttgat tgctccgctg tacaatccga ttggtgcttt
     3781 ctctcctcct tcaatgtatt tcagatctcc tcttgttaat tgtgaagctg caaatacttg
     3841 atttgcatcc tccgacaaaa caatctcttg tgcgtcccaa tcatcgattg cttttttaaa
     3901 ctcttctgaa agtccattcg ctagtcctga caacatgaat acgacatacg caatcaagaa
     3961 cataataccg acgatcaagc catatcgtaa tttagaatag cgaatttctt tgatagctag
     4021 aaacatttgt ttttcctccc tatgatagat tgatagtgac aaaatgtcac ttgttgaata
     4081 gtgtaactct aaagtgacaa tttgtcactt gtgttgtttt tttcttatca ataaattgtc
     4141 taagacatct ttcgataaaa ccttaacatg agtcataaaa aatttattta agccttattt
     4201 atgctttcca tgataaacta aagtgaccaa atcaatgagg tgggaaaatg attaaactat
     4261 tagtagtaga agacgataaa gtattatcca ataatataaa agaaatactc gctgcaatcg
     4321 gcgagataac acaagtttat gatggtagtg aaggactgtt cgagttggaa agtggagttt
     4381 atgatcttgc aatcttggat ttgatgatgc cggaaatgaa tggatatgaa gtgttagcaa
     4441 aaatacggaa agaaaaaata cagactccag tattgatttt gactgcaaaa gatagtttgg
     4501 aagataagat tgaaggattt gaacgaggtg cagatgatta tctaacaaaa cctttctatc
     4561 gagaagaact gatcatgcgc gtgaaagcat tattgaaacg ctcgctaggt ctctttgatg
     4621 aaaatgttct gaagtataag gatatcagtt gtgatttaaa aagcaatgaa gtcttgtata
     4681 aagaacaagt tttgcccata caaggaaaag agtttgaatt gctcgtttat tttttacaaa
     4741 ataagggaat cattttgaca aaagagcaga tatttgaccg gatctgggga tttgattctg
     4801 atacgacgat tacagttatt gaagtttata tgagcaactt acgaaaacat ctgcgaagta
     4861 ctggattgga taaagaaatc aaaaccttac gtaatgtcgg ttatatactg caaagggtgt
     4921 gatgcaatga atggagatat tacacgaaag caacgtagtc gcttttttgc gacaaatgta
     4981 gcagcatttg cttttatctt ttttcttttg ggattcatta ctttacagat attaaaccaa
     5041 tctgcataca gagaaacaga tctgtcatta gagaaaataa attttgattc tcgaacagta
     5101 caattagaga tacaaagata tcagcaaggc gatccctttt taaacccgac tcaaacggaa
     5161 caaaacagaa caaacatgga acagccatta atggaaccat caaatgaccg ttttaacact
     5221 caagtgatct tgtggtctaa aaatggtgag atattgaata aagcagctat tggtgggcgt
     5281 ttcaaccaaa tcaaagaatt aaaaatggat acgaaaaatc tgaataccat ccaaaatatt
     5341 gaattaaatg aagacagtgg ctcatctgcc ctctctttcc gttcgattac gaaaaaagcg
     5401 gagacgaatc aagatggcgt tgcctacatc caaattttag aaaatacaaa tcaaatcaag
     5461 aattcattag cgacttttcg cacgattatg attttgtgta tgattgtttt ttggattctc
     5521 tctattggta tcagttatta cttgtctagt ctaagcatgc gccctattat tctttcttgg
     5581 agaagacaaa aagaatttgt tgagaatgct tctcatgaat tgcgcacacc gttaacgatt
     5641 attcaaaata gtttggaaca tctttttacc aagcctaatc atactattat tgaagaatcc
     5701 gaaagcatcg ctcaagcatt gtcagagacg aggcgcctca ctggattgac ttcggatttg
     5761 ctgacaatcg ctcggaatga ttctgatcag cagctgcttt caaagcaaat gatcaataca
     5821 caagaatata tcgaacaact tgttaaacca tttcaagaga tggccatcat agatggaaaa
     5881 gagtttattc tccataacga tgcaacatta gaagtaccaa ttgatgaaaa aaaaatccat
     5941 caagttttgg ttattttgct ggataatgca ttaaaatata cgaatccaga agataagatc
     6001 attttggatt catctgttaa ccataaagaa tggctgattc acgtaaagaa tactggccct
     6061 tctatcagtg aagaagatca agcaaggatt tttgatcgat tttatcggga agatcaatct
     6121 cgggcgaaag agactggcgg atacgggctt ggcttggcaa ttgcaaaaca ggtaatcgaa
     6181 cagcatcatg gacagatttc tgtatccaat tatcttccac aaggagtcga ttttaaagtc
     6241 caattgccgt tgaagcctaa aaaataatgc ttatccctaa aaaagggctg tgacaaaaat
     6301 cttgtcacag cccttttttg ttgcgaataa acggtgttcc agaagtagct tcttcggaaa
     6361 taagccgaaa tttctttaag ctgaccactt ctgtcacaac ctctttagct tacgcttacg
     6421 attttttatt gcggtcttca atgtgtacat caccgcttgt gtcaatatcc aattcttctc
     6481 ggctaacatc ttctgtcact ttttcaacat cttcatgttc ttctttatga atcccaactt
     6541 cttcacgaat aactgtatgt ttgcttacat ctacttgctc ttctttgatt gggatagtaa
     6601 tcgtgtcatc ttccaatttt tcgtctgttc cctgtgaaga ttgatctgtt actggtttgc
     6661 gttcgatgac gatttcttca cgtttgaccg gtacttctac cgtttcagta tcgttgacca
     6721 catgtttgtg gatgtcgact tctccagttg tcacatcatg tgtatttaca tctaattttt
     6781 cttcttttag ttgaattgta tcatcatcta gatttggttt gcgatcgatg tcttcagtct
     6841 tgcttgtacc cattgtatca gtctcgccag gtacgtccat cggatcagtt cctggtgtca
     6901 tattcaatgg atccgttgta ttttgaaagc ctgtcgtgcc acctacgtcc attggatctg
     6961 ttccagtgcc agtttctaat ccagtcccca ttgtgcttgc tgggctaaca gtatcttgtg
     7021 tatccattgt atcttctgtt gtttctggct gacgatagcc ttttactgtg atgacaaatt
     7081 ttccatcaga aatatctcct ctatattgat ataaaggatc attttctggt gtagccgtct
     7141 cagaatcata ggtatcaaaa gaaaaagtat cttttatttt ttcccataaa gaacggtctt
     7201 cttcaccatc catccgattt tcatctgttt caacatctat accagacaat ccgtcaaatc
     7261 ctagtctaga tgctgcttca tcagtagtat aaataacgat atcatctcgt tcataccctt
     7321 cgttacgcaa ccgttcaatt actgataatg cttcttccct cgtactatag cttccgatga
     7381 ccgttaaatt tttctcttcc atgtaaattc ctcctagcaa ata
//
LOCUS       DAZULY010000143         7119 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000143 DAZULY010000000
VERSION     DAZULY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.144"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..561)
                     /locus_tag="ACG81A_001989"
     CDS             complement(<1..561)
                     /locus_tag="ACG81A_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFS5015336.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(748..1398)
                     /locus_tag="ACG81A_001990"
     CDS             complement(748..1398)
                     /locus_tag="ACG81A_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015337.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="ACG81A_001991"
     CDS             complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="ACG81A_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFS5015338.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2910..3908)
                     /gene="yvcK"
                     /locus_tag="ACG81A_001992"
     CDS             complement(2910..3908)
                     /gene="yvcK"
                     /locus_tag="ACG81A_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFS5015339.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="ACG81A_001993"
     CDS             complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="ACG81A_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFS5015340.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4924..5655)
                     /locus_tag="ACG81A_001994"
     CDS             complement(4924..5655)
                     /locus_tag="ACG81A_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFS5015341.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5659..6924)
                     /locus_tag="ACG81A_001995"
     CDS             complement(5659..6924)
                     /locus_tag="ACG81A_001995"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFS5015342.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
BASE COUNT         2147 a         1511 c         1103 g         2358 t
ORIGIN      
        1 tataaacatc accccaaata aaacagaaaa cagtataagc attataaaaa ttatcaacaa
       61 ccttgatcct tcttttatag attcaggaaa cagtataatt ttagtactag ataaattgtt
      121 tacaccgatt agtgttaaaa tatctttatt cttcttgcta acataaccct ttatgcacaa
      181 aagaacagct atacttaaag taacaaataa ataacgtatt ccaatagaaa aatcactata
      241 aatactctct agcataggac cgacaattga agaagtcact actgcaaatg ctggacctat
      301 ccaagcaaca ttagaatgtt ctttctctct tttcctttgt aaatctttgt tgagtattaa
      361 tctatttatt tcttctgcag gcaagttaat tttcataacc ttatggggga ttatccaatt
      421 aaaaatcgga aaaaggtagc ctccccaaaa agtatagtga tctgtgtcaa tcagataata
      481 acctgatttt gattccagaa taacatatct caaagccgaa cgaaaatttt tcatgaatat
      541 ccataatctc actgtttcca cacctaattc ctccaattcc aattaaatgg atttatagca
      601 tcaccaatta tgttaactgc ctgaccaacc tcattgataa aattggcacc actatcccat
      661 gcttcaacaa tgctatatat ctatcaaaca aaaaatggaa aactaaatac tcgcgcacat
      721 aaattttaca ttctgttttt tataagacta ataataatca ttgaaaaaat ttcttatcgg
      781 tggcgcatat tcccaattga ttaatgtgat cccatattct gcaagtattt tacgagcttc
      841 ttctaagtca tagtgagaaa aatgcataaa acctgcaata cgatcacgga ctgtgagctg
      901 tggcttgctt ccaccatcat tcataaaata tccagcagag ccactactta aagtgattgt
      961 tacatcttca ctttctatta ttaagaattc ataatgaaat gctttcaact ctccactatt
     1021 atttaaaagt tttatactcc aacttcgtct aggttgagtt gtcacattcc ctctaaaact
     1081 tcctataaat tctgcaatca ccgtatcttc aatcaaaatt ctttcctcat ctatttcata
     1141 ttcaagacta aacggaaatt ctccatatgt aatctctggc aatggtggtg ctgtagttct
     1201 ccaaataaat acaataactg tgaatagaaa aaatccaacg atcgatataa atgttatgag
     1261 acctgctcga agtaaaaccc gatataataa aaatgctgta tttcgctttg ctatatctag
     1321 aaaaataaaa agtactgcaa tcataatgat agtaaaagtt gtaaaaaagc acattttttg
     1381 ccctcccttt ataaacatcg attctcaaat tctaaaccaa atcttgatag ttcaagttca
     1441 ggtataacgt ataccaacca tacatcccaa ttactattta tgaacttatg attatcactg
     1501 aataaatcac tccatcccct tgttaaaact tctacattaa ttacacctgg ttgttttcga
     1561 ctagctaatc caatgaactt atcaaccatc cttatccacc aaccacgaga atcttgacca
     1621 gaagtaagaa aaacagttct caaggttgat cgagcagttc cttccagttt ttaattaatc
     1681 tccagttata gataaatact tgatcctatc tatatcagga cttctatatt aagcaaaagt
     1741 ttatgtataa aactaaaatc ttcattcagt tgtctccccg ctttttatac gattgtatta
     1801 aaaaacaata gaagcctttc aacattatgt tactccagta aagttggaat tatagtttga
     1861 cggaagaaaa gcaagtatag cttttaaaaa aaactgatac attcgcatct cctgcttcat
     1921 gtatcagttt tttctttgga ttaaactgct tcttcacgca gtttttccgc aaattcattt
     1981 attttgcgaa ttcggtgatt aattcccgat tttgatattg cacctgaggg aatcatttcc
     2041 cctaattctt tcaagctgac ctctggatgt tgaagccgaa gttctgcgat ttcctgaagt
     2101 ttttcaggaa gggcatgtag accgaccttt gcttcgatca attcgatgtt ttcgatttgt
     2161 ttagaagccg catcaatcgt cttgttcaga tttgctgtct cacaattgac tagacgattg
     2221 accgaatttc tcatatcccg tacgattcga acatcttcaa atcggagcat tgagttggtt
     2281 gcaccaatca atgttaagaa atcagcaatt ttttctgctc cttttaaata cgaaatatag
     2341 ccattgcgcc gttcaagcgt tcgagcatgt agaccgtaat aatttaacat atcgcaaatg
     2401 tcttggttat gctgttcata aatagaaaat atttccaaat gataacgact cgtttccgga
     2461 ttattgactg aacctgaggc cataaaagca ccacgtaagt aagaacgcat tttttgcgaa
     2521 tttcccataa tctcgtccga aacgtgtcca ttgaacatca taccatccat gatatcaaga
     2581 tccatcaaca catcttttgt gccttgcttc aaccggacga tatagacatt gttctttttt
     2641 aatttcattt ttttacgaac gagtagctca cttcgtacct gataatgatc tttcaaaaga
     2701 gaatagatac gtctggcaat tgcagcattt tctgtttgga cgttcaaaat aaactgctgg
     2761 ttcacaatac ctaaagagcc gttcattcgc agcaaagcag ctaattccgc tttcgcatgt
     2821 tctcgatgaa cttccaaacc agttagttct ttcttcacat ctgcagcaaa tgacatccta
     2881 ttctcctcct ttctccaaca ataaattgct taatattttg aaccgaaaac taaacggaac
     2941 aattcttcta ctactttgtc tccatcgtgg aagacaccac catctttcaa ttctaaaaag
     3001 tcggtggaaa tcactcgaca tccttcattt ctcaatccgt tgaagtcgtg tgcgacttgt
     3061 actagatact catcatagat ctctgggtcc atataatctt caggcacttt ttccgtattg
     3121 accaatactg tatcgataaa aggctgattc aaatgcttgt gtaaaacacg tatatgatcg
     3181 gcatctgtga aatgttccgt ttcccctttt tgggtcatga tattacagat atagacggtt
     3241 tcagcagttg tcttgagcat tgcttcgccg atttctggta tcactaaatt tggaaggata
     3301 ctggtgaata aacttccggg tcccaaaacg accatgtcgg cgtccataat tgctgaaacg
     3361 acttttcgag ccgctttggc ttcactttta tcatttgtgt tgcagacata cacacggtca
     3421 atggttttac ggttgatcgc gattttagat tctcctactg cagtcgttcc gtctttaaaa
     3481 acagcatgca aggtcaaagg agtttctgaa gaaggataaa catgtccatc tacatgcatc
     3541 atttttgcta ataattgaat cgcctcataa gtgcttcccc gcatctcgga aactgcggca
     3601 atgatcagat taccgatcgt atgattggct aaaaactgat cttccttttt gaaacggtat
     3661 tggaaaatat cttcatagaa ttgtggcata tccgataacg ccactaagac atttctcaag
     3721 tcaccaggag gagccatagc gatcgattct ctgattgcac cgctgcttcc accatcatct
     3781 gcaacagtca ctactgctgt gatatctgct ccttgatttc gtaaactttt caggataact
     3841 ggcagaccag tgccaccgcc gaccactacg atctttggtt ttcttatccg gtaggttttc
     3901 attttcacga ccggttcacc gtctctttcc ttttatcctt gtcccgatgt gtcacattga
     3961 ctttatagtt tttccctaaa gcatttgcca aacgttcagt caatgccaca gaacggtgtt
     4021 gaccgcctgt acagccgatt gcaactgtca agctgctttt tccttcttta atgtatccag
     4081 gcaatacagt ttccagaaga tccaaaaatt tatggtagaa tgattgcgtt tcatcaaagg
     4141 acatcacata atcatagact ggctgatctt ttccggtcaa tggtcttagt tccgggatat
     4201 aatgagggtt gggcaaaaaa cgcacatcca tcacaatatc cgaatcgatc ggcaacccgt
     4261 atttaaaacc aaatgataca aattcgatcc gaaatccttg gtctcccttt gatttgaatg
     4321 actcattgat tttttcacgt aattgtcgtg gtgtcaatgt tgtcgtatca atgatcacgg
     4381 aagcttgggc tttcaagtcg tctagtatcg cccgttcttt acgtatccct tcagtgatca
     4441 aaccgtccat cgccattgga tgtgctcgtc ttgtttcttt gtagcgagcc actagttctt
     4501 cgtctgaagc atctaaaaat aagatagaag tgtcgatgaa gttcgtattt tcgatttcga
     4561 tcagcatgtt ttggatttct tcaaagaagg aacgagaacg caaatccact cctaaagcca
     4621 tttttgtgac tttacctgat tctttgatca attcccagaa ttttggaatc aatcgaggcg
     4681 gcatattatc gatacaaaaa tagcccatat cttcaaaact ttggatagct actgttttcc
     4741 ctgcaccact catacctgtt atgatgacta attgcaaatt atctgtcata tttacgaacc
     4801 cctctcacat tgtgcataat aaaattatat catcccgggc gtatcattac cagttttctt
     4861 tttcttttac aaaaagattt ggaccaaata tcttttagga tatttgcccc aaatccaatt
     4921 tttttacttt tccgatgctg tatccaatgc ctcgcttctt tcagctaatg cacgttcgat
     4981 cgttcgatcc gctaaaatag attgagcatc gatcactggg taatgattat cttcagcaaa
     5041 ttcatgcatc aaagataatt ctgtgcaacc taggatgact ttttcacagt ttaggcgctc
     5101 taccgcttcc tctaagattt cgtaatagag ctcttgattc aaataatccg attccttgat
     5161 ctcatgatag atcaggtaat tgattttttc ttgaagcgca gtatcaggaa tcactgtttc
     5221 gaaaccaaga tttttgactt cgcgttcata gattccagct ttcatacttc cttctgttcc
     5281 tagaatcgct actctccctg tcgtatgctg acggacaagt tcatttgctg cttctcttgg
     5341 catatgcaag ataggaatat ccgttgccgc ttgtaactct tcaaaaaagt agtgtgcggt
     5401 gttacaagtc aacacaatga agtttggtcg caataaattt tgtttctcaa tatcatccag
     5461 cagaaacggc attggatttt cttctgatcg atctaaaata taagctgtac gatcaggaac
     5521 tgttgcatga ttgaataaga cataattcaa atattcttga tcttttgtag ctttcgttcg
     5581 gtggttgatc aagcgaacaa aactttctgt cgccatcgtg cccattccgc ctaaaatact
     5641 gaaaaaattc tccatgaact agccttcttt cacatgaaaa tactttttga atcttggaat
     5701 atagttatgg aacatatatt tcatcaacaa ccagcgcttg attgatcggt ctttttcata
     5761 aaatacagta gtaccgtaac gattttcttt gatcatttgc tcagctagtt ttttgtcttc
     5821 attttcccga gcatattcta aaaagatttt tttgggcaca cccatccata atcttgcttt
     5881 atctgattga ttcgttccat aggtcgtttc aacaaagggt ttgttgaaca ctcgatcttc
     5941 tgtgacaaaa cgagccagat tcaagccgtt caatgtcaca aagaaactgc tacgaccttg
     6001 tctcaaattg atttcaaata atttatattc tccatctcta ggatcatact tcatatcgaa
     6061 attggcaaag cctgtatatt cgattttttc caaaaatgcc ttaatggttt gatagatttt
     6121 ttcgttataa tctggcatga tcacaacgta attaccaatc gatgctggtg taggatcttc
     6181 taatagtgga tgaccaaggc acatcatccg aacttgatga tcttcatcta catacgcatt
     6241 tagcacacgc atattactat catcacccgg gataaaatct tgtacaatca tttcactttt
     6301 ataaccggct tcgtatatcc ggcctaaaat taaatcgaac tcttctctag tctctaaaat
     6361 aaaagctttt tttcttcctt caaattggac agaaagatac tccacactgt ttgcaggttt
     6421 caaagcaact gggaattcaa aaggcaattc ctgttccaaa tgtccattta ctaacatttc
     6481 ttctctgacg atcaatgttt taggatacgg aagatcatat tcttcacaaa cttcataaaa
     6541 gcttacttta ttgatcagcc tttcaaataa agaataatca atatatggac agataaaata
     6601 ttctgacagc tcttgtttat gttgggagat caattctgca tatccatcac cacaagcaat
     6661 caaaagatat tttttactct tatcagaata tctttctttt gccaaacgaa gcatcgtctc
     6721 gataaataca gggtctttat caaatcctgg aataacttct acattgacaa ttttactgta
     6781 gcgggtaggg gctaactgat ccgaagcata tgcttggcag attttgccgt atgcttcatt
     6841 aaaagaacgt gccataccat aaacgttcat gtcgcttcct aataatatag ggataaattc
     6901 ttcattttca atactgttca tcattatctc tcacttactc ataatctctt gtttttcttt
     6961 tccaactaca caaggaatat cataccataa agaaaaaagc aatcaaagga aagtcctccg
     7021 attgctcaat tcttttttga acggattgaa cttataggaa gtttttatct ataccaatca
     7081 gtgtagaaag aactaccatg taatgattct agaacactg
//
LOCUS       DAZULY010000144         6999 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000144 DAZULY010000000
VERSION     DAZULY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.145"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..545)
                     /locus_tag="ACG81A_001996"
     CDS             complement(<1..545)
                     /locus_tag="ACG81A_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015343.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAW"
     gene            complement(645..1154)
                     /locus_tag="ACG81A_001997"
     CDS             complement(645..1154)
                     /locus_tag="ACG81A_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFS5015344.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(1355..2224)
                     /gene="scrK"
                     /locus_tag="ACG81A_001998"
     CDS             complement(1355..2224)
                     /gene="scrK"
                     /locus_tag="ACG81A_001998"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS5015345.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(2244..3365)
                     /locus_tag="ACG81A_001999"
     CDS             complement(2244..3365)
                     /locus_tag="ACG81A_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFS5015346.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(3367..3762)
                     /locus_tag="ACG81A_002000"
     CDS             complement(3367..3762)
                     /locus_tag="ACG81A_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5015347.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(3781..4578)
                     /locus_tag="ACG81A_002001"
     CDS             complement(3781..4578)
                     /locus_tag="ACG81A_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5015348.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(4580..5368)
                     /locus_tag="ACG81A_002002"
     CDS             complement(4580..5368)
                     /locus_tag="ACG81A_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5015349.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(5382..5858)
                     /locus_tag="ACG81A_002003"
     CDS             complement(5382..5858)
                     /locus_tag="ACG81A_002003"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS5015350.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(5930..>6999)
                     /locus_tag="ACG81A_002004"
     CDS             complement(5930..>6999)
                     /locus_tag="ACG81A_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5015351.1"
                     /translation="KRLALILAHGDLTATCIASTANRIVERYIYDAIDMPLMTSSSEI
                     ADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTRMAIDVANQ
                     ILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKKILEDSFPR
                     DTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIEDMILGMNIS
                     KFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDAIYILQNDL
                     GISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFKQSFSVVEH
                     YYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         2175 a         1483 c          984 g         2357 t
ORIGIN      
        1 atccaagccc agtctaaata attttttaaa atctcatcat attttgcaaa tatttcattg
       61 ccatattccg tccttaaaat tatattttca gtgccagaca agtgatttat attgagttga
      121 taaaaaagat ctccatctaa aaaaatatca ttagaagaag atttccccac agtaatgacc
      181 attccatcag ctctatgtgt tacatacaaa taattgtatg acctcatttc acctaatgaa
      241 tcaattattt cttgcccttt agctctaata tattgatctc tattacctat ctcactctta
      301 tttaacaaaa gttttttcat gtcaaattta atcggctgtt ttatttttgt aaattcgctt
      361 ctactactaa tccaatccga gattaatttt tcaacatgtc ccagagtaaa ctctgattta
      421 ttcgctaaat actttttttg ttgtattaaa gacatctttc cccgaagctc aaaattgagc
      481 ctacatcgat tctctatctc ttcaaaaagc atcttatctt catcattctt atattcatca
      541 ttcataaaac caatccagct cctcgattaa gataatattt taatctcata caaaaactag
      601 ctctcatcaa aacaaccatt gttttgattt ctcatgaaat ttctttatac ttttctccga
      661 ttataaattc ctaacccctt gagaaaatta cgcatatatt tatctccgca aggcttaaaa
      721 tttcgttggc cttcttttct caaaaatgcc ccgatttcgc cttttgatgg atagatttct
      781 gcctcagcaa aaacgtctaa aatatcatca gtggtcatag acaaggcgat ttttaatttt
      841 tttagcaaca cattgttgat atatttgatt tccttgcttt tttgcggcgt gtctactatc
      901 ggcttatctt tttgcggacc acgcgccaag agaatcaggc catttaggaa tgcgtcaaaa
      961 acttggttgt tgatggtttt ttcatacaca ttattttctg cattatcttt gttcttatcc
     1021 tgtactttgg tcagtaccgc ctgagcttct tctttagtca gtggtacacc gcctagctca
     1081 aaagctttaa tcaaatccgt atctttcaaa tcaatcgcat aacgcaatcg caataaccta
     1141 tcattgttat tcatttgttc ctccgaagta tttagtcttt actttcttac gtactttttg
     1201 tcttgctaag atttttagga tcaatgataa aaaccgcatc acttatgata aggcgctggg
     1261 tatcatgagt gaatgatatt gctgaataca aatagtccga ccacattaag ttgatcggac
     1321 ttcataagca ttttattgac taatactttt ttgcttaaga attgattcta acatcttttg
     1381 ctaaagttaa gcatcctata gttcctgcat tatctgctaa cttaggtgtg acaatgtatt
     1441 cttcgatatt tggcgtatcg acatagccgt taattagttc taaaaaacgt ctttgtactt
     1501 tctttttcag atgttcttgt tgcatgacac caccaccaaa aattataata tctggtgaaa
     1561 gcatcagggt cgtattacag gcacattgtg caatataatc cgcctcgatt tcccagaacg
     1621 gatcttctat agaaagatct tgtcccttgg ttcccgtacg catctcgata gcgggtccag
     1681 atgccaaccc ttcgagacaa tcatgatgaa aaggacaagt accagagaaa gtatctcctg
     1741 aatgtctctt aactaacgta tgccccattt ctggatggct aaacccttca acaaaaatcc
     1801 catcttgtat agctcctcca ccaattccag ttccaacagt gtaataaaca acactggata
     1861 aaccttttcc atgacctgaa acatactccc cgtaacaagc agcattcaca tcggttgtcc
     1921 aggcaattgg aatttcgaaa tgcttattca acgttccaac aaaatcaaaa tttttccagg
     1981 cagtttttgg agtagaggtg atatgtccat aggtctttga ttcacgatgg atatcaatcg
     2041 gtccaaatga ccctactcca atgctttcta attgttcttt atagtgatta aagaaatcaa
     2101 ttaccaaact cattgtttca ttcggtgtag ttgtaggaaa actcactctt tctaaaacct
     2161 ttaaatcatc attgccaatg gcacaaacaa atttagtccc acctgcttct attgagccat
     2221 acatttctta ctccaccgtc cttttatcta ggaattcgtt cttccctgtc taatagtttt
     2281 tcgaaaaact catgtggttc cttttggtac caataggcaa cagaagaaat atcgtcttga
     2341 cgctcataaa gcttgatgtc atcatttcct agggtttgga aagtaacacg taaattttct
     2401 ttgaaactaa tggggtcggg taaatgccag cggtacaacc catgcatcgg taaagcatca
     2461 tcgccaaaac catgaactgg atttggatta ggttttccag cactaaaata atcccgggtg
     2521 tgatctcttt tagattgaaa aggatagccc ataaatagcg tattaaaatt ttgagcatat
     2581 gccctttctc caaagttctt tttatggaaa gcccaagcgc cgccaaaata atcttctgct
     2641 cctgtagatg ttattgtagg gaaatcttca tctccatcta tataaaactt aaattctcct
     2701 tccccccacc agtatctttc caatgcgcac aaagcaaagt aggttcctac ataataccca
     2761 gtgcctttga ttccatcaat aagggtgtaa tcttttcctt ttgtcgtgat gggttcacgg
     2821 ttccatttag catgaaaata catcgcatca tccggtactt cagtcaatgc ataattaaat
     2881 gtataaaaaa tgtgcttaat aaactcagga tgttcattcg ttaaagtaat cttagctttt
     2941 tttcgaaatg gcatctcaaa atagcaattc atccctgatg ttggattcac tacgattggt
     3001 aaggaattca cttcgcatct tgtcccaaac ccattacaaa aaaaatctcc taatggactt
     3061 tctacagaag gagtggattc atcatcccaa taaatacgca aaattaaatc acgtaagacg
     3121 aaactcccag cgattgtttt gtctgtcact gtcatccaaa aatgacgtaa aattccaggg
     3181 ccttccattt ctgctaaagt aattgtctct ccagcattta tcggaacaga aggtgaccct
     3241 tttctcccaa ttcctagatg actcgctgtc attgccgctt tccctttttc accattttta
     3301 ttttctggtg aaatagttct actttcctct cccttgtgga gccatatatt atttaaacaa
     3361 ttcattttat tcgtcctcta tcctttcttc acttttttga ttattcatat agatgatttg
     3421 ttgttgtgcc tccgcaataa ttgctccaat atctaaatta tcatttccta actgcaaagc
     3481 gattgacatt gccaagggaa gattcattcc tgcaattacg tgcactctgt cagaaatata
     3541 ggtaaaaaac ttttgattta cacttcctcc agccatgtca gttaaaacaa ttatttcgtc
     3601 tgtagtatcc actaactctt taatttgttc atctatatca tcaatttctt cgacatatgc
     3661 acataagcta ctaatttgat tatgcatacc tgttaaaaac tctagtgtct ctttaaaacc
     3721 ctcagctagt attgaatggc tagcaatgat caatcttctc ataattcaaa tccttttcct
     3781 ttacgctaat attccaaatg cagccataac catcgcaaaa acaataacga gtagaatcaa
     3841 aatggtcata ctgatttttt tcttactgag tagataatat gctgcagcag taataattac
     3901 tggaattaat gttggtaaaa ttttgtccaa cattggttgg attttcatac taacttcacc
     3961 aaaagtgaat ttaagtggcg ttgaaacact gataactgaa gcaattaatg atccaactac
     4021 tgttagcccc aatactgaag cagcttcagt taaataatta atcttttcac cgtattcagt
     4081 aacaatcttc attccctgct tataaccaaa ttcaaagcct ctcattcgga aaaagaagat
     4141 taccatgatt aaaatgaacc ataaaaagat tcctacagga ttattttcta aagccatata
     4201 agccgcaatt gatcccatga tggttgggat catcgtcatc aaaagagagt ccccaatacc
     4261 agcaattgga cccatcgtac caactttaaa gtcattaatc gcatcttttg cttctaatcc
     4321 ttgcttttcc tccattgcaa cacaagcacc taaaataatg gctgccagcc aaggctgtgt
     4381 attgtaatac tgaaagtgat tatttaaagc ttcgatgtat tcatcatcat ctttgtataa
     4441 ttttcttaaa ataggtgaca atgcgtaagt cacagatgca ccttgctgtg tttgataatt
     4501 aaaggtacaa actgacatca accaacgata gttcgctttc ctaagatcct ttttcgttac
     4561 ttgatatttt tcgctcattt tattcatcct ccccaataaa cccaacatca gaagctcccg
     4621 cagaagcagt gatttgtgca tctttttcag ctctatgata cacattaaat gctaaagcga
     4681 atcctatgat cgccgctcca ataataggta ccttcaaaaa agcggctaag aaaaatccaa
     4741 caagtaaata actaatgtat cgacctgtag gcataaagcg taacaacatc gcaataccaa
     4801 cagcaggcaa catttttccg gctaatgtta gcccatccat aaaccaatta ggtaaaacat
     4861 ccaaaacgta attaacagca gactgtccta aagtaacggc aataaatact ggaattgctg
     4921 cagataaacc aaagaaaatt ggccccaacc acaaaacact attcattttc ttaaattctc
     4981 gtttattcgc atattcctgt gatttgcgaa caacaaaacc atttaagatt ttgacaatta
     5041 catctagttg cacccctaac atacctacag gtagtcctat tgctaatccg gcttctactc
     5101 caagctggct accaaatgcc gttgcaataa ttgcgcacgt tccatagtcc ggtacagatg
     5161 agccaccaaa accggcaact cctaatgaca tcaattgaac tgtaccgcca actatcaaac
     5221 ctgtttttat atcacccatc aaaattcctg tgaataatcc ccaaaaaact gcgtaattta
     5281 caaagatcat cggaatccca taataatcaa catgtttaat aaatgctaat atagtcagca
     5341 taataatttg aaaaatagta aaatgcatta tccttactcc tttatcctaa ttttgattga
     5401 atttcctgtg ccttatccgc cggaacaaat tgtgtaacaa tttctacacc agaatccaac
     5461 aattttttat acgtgctgat attttcatct gatgcatagg ctctagaaga tagctgtaat
     5521 gcattatctt taacaacagt accgccaaca attagtcggt caattctaat accgttatct
     5581 actaaatgaa ggattgtttc cggctctttc acgatgacaa atactttttc ctgatcgtat
     5641 ttcccctttt taaaattcgc aatcgctgtt tcttcagtga taattgatac agccattcca
     5701 gcaggtctag ctaaacgcat acttgatttt tggatgtcgt tatttgcgac agaatcatcg
     5761 atcaccatca ctctgtttgc tttagacatt ggtgcccatt gcgtgacaat aatcccatgc
     5821 aataaacggt tatcaactct cgacataaca atactcataa atacttcctc ctttatcttt
     5881 cattttctta caagcaatta ccatgccaaa acaaaaagaa cttgataaat caagctcttt
     5941 ttacataatc ataaacgtat ttaacctcat gaatcgggat gtcgacacta taataatgtt
     6001 caacgacact gaaagattgc ttaaaatgtg tctgaaattt ttgaaattcc tcactcgttt
     6061 cattgaaata aatttcatct tccaaagtat tttgttttac taatcgttca attaagcaac
     6121 taatgtgaac atataatccg aaacacgtat tattatcaaa tgaaattcct agatcatttt
     6181 gtaagatgta tatagcatct gaaacccgtt ctaataattt agtcggattt aatatggtca
     6241 attgctccat taaattcgtt agactaaaat tcttcagaat attattgttc aaaactttca
     6301 gttcattatc attaaagtgt ctattcaatt gattggagaa tttacttata ttcattccta
     6361 aaatcatatc ttcaattgaa aagaattcta catcttctag atgtggattt agtgttccaa
     6421 caataaaaag aatattgttc tctttacgaa gctccgataa atagttttca ttcactaaag
     6481 agtaatagtc acacgtttct atgacaagat cagtatccct tggaaagcta tcttcaagta
     6541 tcttttttaa tttaaacgaa gttcctaatc cgcttgcaca agaacagaca atcatatcct
     6601 tcttaatatt tgatgattca taaatataat ccacattttg attttctttt acttgttcaa
     6661 gaatctgttt aagcggtaaa tctaataata tctgatttgc aacatcaata gccattcttg
     6721 ttgaaacatt gtttagtagt ccgaattttt tttgagcgtc tagttccaaa tttagataaa
     6781 ttttctccag tgaacccata tcaacaagta aaataatttc ttcatatccg gtacactctt
     6841 gtaagtatct attaattttg tctgcaatct cactacttga agtcattagt ggcatatcaa
     6901 tggcgtcgta tatatatctc tcaacgatcc tattagcagt acttgctata caggttgccg
     6961 ttagatctcc atgagccaaa atcagtgcta aacgttttt
//
LOCUS       DAZULY010000145         6936 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000145 DAZULY010000000
VERSION     DAZULY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.146"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..1151)
                     /locus_tag="ACG81A_002005"
                     /pseudo
     CDS             complement(1..1151)
                     /locus_tag="ACG81A_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1394..1849
                     /gene="tnpA"
                     /locus_tag="ACG81A_002006"
     CDS             1394..1849
                     /gene="tnpA"
                     /locus_tag="ACG81A_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFS5015352.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2114..2212
                     /locus_tag="ACG81A_002007"
                     /pseudo
     CDS             2114..2212
                     /locus_tag="ACG81A_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2435..2665)
                     /locus_tag="ACG81A_002008"
     CDS             complement(2435..2665)
                     /locus_tag="ACG81A_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFS5015353.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2895..3713)
                     /locus_tag="ACG81A_002009"
     CDS             complement(2895..3713)
                     /locus_tag="ACG81A_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5015354.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3874..4563
                     /locus_tag="ACG81A_002010"
     CDS             3874..4563
                     /locus_tag="ACG81A_002010"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFS5015355.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4577..6079
                     /locus_tag="ACG81A_002011"
     CDS             4577..6079
                     /locus_tag="ACG81A_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5015356.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            6092..6568
                     /locus_tag="ACG81A_002012"
     CDS             6092..6568
                     /locus_tag="ACG81A_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS5015357.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6665..6775)
                     /locus_tag="ACG81A_002013"
                     /pseudo
     CDS             complement(6665..6775)
                     /locus_tag="ACG81A_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2252 a         1092 c         1265 g         2327 t
ORIGIN      
        1 ccaaccttga tctgagtgaa acgtacgacg atagggacaa tcgttagtgg tctggatagc
       61 ttcctccaaa ccttccatta ccgctaatgc attgggcttt tcagagatac gataggataa
      121 aatctcacta ttgaacatat ccataaaagg atctagatac aatttctttt gacggataat
      181 gcctttggca tccacttcat ggtatttaaa ttcagttgta tccgtcgtga ttttctgatg
      241 gcagatactg gtgtagaaac gacgatggat ccgatttttg gctactgctc cgacttgtcc
      301 tcgataagaa ttgtagcgcc gtgatttacg tgtgtatgtc gttactttta ttcctagctt
      361 cttgatcagg cgctgaactt tctttttatt gatagagaaa ccgcggtttc ttagttcatt
      421 ggtcatccgt aaacagccat aatccttgtg cttctcccga actttaagca tttccgcttc
      481 aatctcttga tctggatttt tacggtccaa tcgtttttgc cagtacataa agattgcctt
      541 agggaacttt aaagtttcta gaagctcaac taatttaaac ggtcctcgga ggcttgcgat
      601 gattcgtgcg attgcttcat tcgtcgttgt tgggcttcct gttttcgcag cttcctcaat
      661 tcttttaaaa aggcattttc aatttgaagg gaacggactc gtttctccaa ctcctcgatg
      721 cgttcacgtt ctttagacgt tgctttagtt gtagattttt tcttattttt gggttcattt
      781 ttcttagaca agataggtgg acatcctttc gtttttgaga gtccatcgat cccaccctca
      841 cggaattgac gcatccaact agcgatcaaa ttagggttgt tcatgttaag tgtgttggca
      901 acttcacgat aggacagctc acttgttaga tataactcta tcgcatccag cttaaattga
      961 acagaatatt ttttcttttg tcgctttctt actagaccct cttcaccaaa ttcttgatag
     1021 gcattgatcc agcgcttcac ttgtgatgga gccttaatgg aatatttctt tgccagataa
     1081 tcacttccgc ctttaccatc caagtaatca tgaacgatct ttaatttaag ttcaaaagta
     1141 tacttagcca taaaaaatac cgacctccaa aagttagatt ttttggtcta acttttgggg
     1201 gtcggtacaa aacttaaaag ttttagaccc ttttattttc ttaatgactt cagtcagttt
     1261 tgtgcgtaaa gcgtgcgaaa caagaaaacc gcgcgtttct acgcgtggaa gaagtatctt
     1321 tagcgataaa aactcctttc gatatactag tgagtggcta ccaaccgctc atcagatcga
     1381 aaggagtttt ttcatgtcaa atgacgataa aagtttagca tatactcgtt ggaattgtaa
     1441 gtatcatatt atttttacac caaaatatca acgaaaagta atctatgggc aattacgaaa
     1501 agatcttgga aaaatactta ggaagttgtg cgaaatgaaa gatgtggaaa tcatagaagc
     1561 acatgcaatg ctagatcata ttcacatgct tgttcgagta ccaccaaaaa tatgtatatc
     1621 cagttttatg gggtatctga aaggtcgtag tgcggtacta atccacgagc agcacgcaaa
     1681 tctcaaatat aaatacggaa acaaatcctt ttggtcgaag agatactatg tgagtacagt
     1741 cggattgaat caaaagacaa atgaaaaata catccgtgaa caagaagctg atgatcgcat
     1801 tcgatatatt ataagtaaaa gagagtacgt agaaccattt aaaaaataaa gagaagtaag
     1861 tgagtgaaga tgagcagttg gcggtctttt aaaaatcccc ttttaagggg cagcgagtgt
     1921 cgaccccttc tagggggatt aaaaatacac ccgtcctcac gggtgtatgg gttactgata
     1981 gacttgattt taactggtgt cagaaactta tgtccagaaa gggtgatgaa attttggcaa
     2041 tttacggcta ttcaagaatt agcacaagca accaagatta caaaacacaa attcaaacat
     2101 tagaaaatgc tggcgcagaa ataatcttcc tagaaaatca ctggattatc gaacaccttt
     2161 ggaagtattt ttgagttacg taagtattga tgatctgtct aacttaattt gacaattaag
     2221 agtatattta cttcagatgg taaggcaaca aaataaaata caataggcag atgaactaat
     2281 aaataaaatc tgtattaaat acagataaat taatataaaa atcaaaagga tcataaactt
     2341 ttaacaagtg atgaaatggg tggaaattga ttcttataga cagtttgtct cataacattg
     2401 aagtattttg caattttagc attgaaacac ttttttaatc ttcaacgttg ttcttataaa
     2461 caagatgatt gggaatcttt tgaattaaca ctgtatagct tagaacaccc tttatagaaa
     2521 acattcgctt agcaatctta ataattcctt gtgcccgatg tcgtttagat tcagtaggtt
     2581 cctgctcatc gatcatggaa aaaatttgaa ggatcagatc cttaataaat ttatttaaga
     2641 gaggatgatc cattgcttca tccatactcg ataaactagt gataatgagc tacacgtttt
     2701 tctttcaagt tgatcaatag cttccacgat aaaaatatct ttagataaaa tcaagaaccg
     2761 taggaactac agggatattt ggtcaataag agccaccgct attagtaaaa aataagctat
     2821 caagtatgct ctcatcccaa gaattgccta cttcaatcgt taaaaaccgt taggtttagc
     2881 taagtggggg tagttcactc aatactttta tcaataatag agcgcaaaac tttttctctt
     2941 aattcaactg aatcaatatt attgttttgt tcataaccat gaactaatag atcacaaata
     3001 taaagttgtg atatttttcc agctaaagat ccaccattaa gaaattcttc aatggctgtt
     3061 tgtaaaacta agtctgcaga ctttcctata ggtgaatgaa tatagttagt tatagcaata
     3121 atttttgcgc catttttttt agctattttc aatgaatcat aagtatcttt cgttttccca
     3181 gataaagaaa aaatgattac taaatcatta actgttaata aagaagcgac ttgggcttga
     3241 aaatgagggt ctagtacagc ttttgcttga actcctacgc gaagaaacat attttctaaa
     3301 tcaagactag tatttccact agatcccact ccaaatatat atatgttttt tgattgagtg
     3361 atcattttaa tggcttcatt caatttttct tcgtctaaaa gcctaatagt ggaatagaga
     3421 gcttctgtta aactattagc aatttcatca tagtacttta cactagtcgg atgttctttt
     3481 ttttgactaa agtcttcctt tgcaatttca atttttaaat cagaaaaacc agaaaaacca
     3541 attttttgac aaaagcgaat aatagtagca tcgccaactt ttgtgttttt cttaatatca
     3601 ctcatcgtac tataaataat agttttccca gaatttaaaa taaaatcagc gacttttttt
     3661 tcagacttcg ttaaaaatgg ataattttct ttaattaaat agatataact catacaaact
     3721 cctctacaat ttcaactttt ctccattata ctccaaaata ataaaaaaaa ctcctaaata
     3781 ctccattttt atctttactt ttatgaaagc ggatgctata ttgtttttgg agttgttatc
     3841 caaaaataat aaaaaattgg agtgtttagg ataatggata aaaagaggtt tttagatctt
     3901 atttcaaaag gtttgattgt ttcctgtcaa gcattacctg gtgaaccatt ttacaaagag
     3961 gaagaaagtt taatgcctta ttttgctttg gctgctcaaa gggctggagc agttggtata
     4021 cgtgcaaatt ctgttcgaga tattattgaa attagaaagt gtgtatcatt accaatgatt
     4081 ggaattatta aaagagatta tccaccagaa tctccattca ttactgccac tatgagagaa
     4141 gtagatgaat tagtagaaac cggtgttgag gtaatcgctc ttgattgcac tcttagaaat
     4201 cgacatgatg gactatctat aaattctttt attactaaaa taaaagaaaa atatcctcat
     4261 cagttattta tggcagatat ttctacattt gaagaaggca aaaatgcata taaagcagga
     4321 atagattttg taggaactac tttaagtgga tatactgagg aaagtaaaaa acaagatggg
     4381 ccagatatag atttgattaa aaaattggta gaagaaggga tttgtgtaat tgctgaggga
     4441 aggattcata ctccagaaca agcaaaagag ataaaaaaaa taggagttgc aggtattgtt
     4501 gtgggtggtg ctattactcg accacaggaa attgcatcaa gatttgttaa agcattaagt
     4561 tagggggaga aataaaatgt ttaaaaaatt ttcacaatta ggacgcgctt tcatgttacc
     4621 aattgctata ttaccagtag ccggattact attgggactg ggaggagcgt taacaaatga
     4681 gagtgctata aatgcttatc catttttaga tcaaccgtgg ttgcatacaa ttttaagtat
     4741 tatgagttat gctggaaatg cagtttttgc aaacctagca ctaatttttg caattggtat
     4801 tgctgtagga cttgcaaatg gagataaagg aacagctggt ttagctggtg gagtagctta
     4861 tctagtatat actgcaacta ttagtgggtt tcttgaactt ttttcagcaa aagatgcaac
     4921 actagataca ggtgtagtgg gatcaattgt tgttggtggt actgttgcat ttttacacaa
     4981 tcgttataga aaaattgaat taccacaatt cttaggtttt tttggaggat caagatttat
     5041 tccgattgtt tctagtttag ctgcaatcat tattggttct ttcttttatt taatttggcc
     5101 accaattcaa aatggtttag tagttgctgg tgaacaaatt gcccaaatgg gtagtttagg
     5161 aactttttta tatggttttt tattgcgttt aacaggtgct gttggactac accacaccat
     5221 ttaccctttg ttctggtata catctttagg tggtaccgaa actgtagctg gatcaacaat
     5281 tgctggagcc caaaatattt tctttgccca actagctgat ccaaaccaca ctggtctgtt
     5341 tacttatggt actagatttt ttgcaggaag atttgcaaca atgatgtttg gattaccagc
     5401 agcatgttat gcaatgtatc gtgcaattcc taagaaaaat cgtaaaaaga atggtgggct
     5461 ttattttagc ggggccctta catcattttt aactggaatt actgaaccag ttgaatatat
     5521 gtttttattt gttgctcctt ggctttatgt gattcatgca ttcttagatg gattatcttt
     5581 ttattttgct gatatcttaa atattcgtgt agggaattcc ttttcaggtg gtttgatcga
     5641 ctttttatta ttcggtgtac ttcaagggaa tgacaaaact aattggttaa aattaattcc
     5701 ttttggtatt gcatgggcat ttatatattt ctttgttttt acattcttta tcaaaaaatt
     5761 taaggtagct attcctggaa tggaagaaga atcagaagtt cattctgaaa aagtaataaa
     5821 caaaaataca tcattacatg aagagtctct tatcattatt gaagctcttg gtggaaaaga
     5881 aaatattgag tctgttgaag cttgtgcaac tcgtttgcgt gttgctgtta cgaatgggga
     5941 gattattcaa aaacaaacaa taaaagacac tggtgcaact gctgtcttcg aagtgaaggg
     6001 tggcatccaa gctgtttttg gagggaaagc tgacttatta agtcaagaaa tcaatcaaat
     6061 attaggaaaa gataattagg aggtttttaa tatgtttgaa attttcaaaa agaaaaaaca
     6121 agaaactttt tatgcacctt gttccggaaa aatcatttcc atcacagaag taaatgatcc
     6181 tgtcttttca caaaaattac taggtgatgg ttttgccgtc atacctgatg gttcaaaaat
     6241 ttatgctcca ttggctggtc gagtaacaaa tatatttcct actaaacatg ctattaatat
     6301 aaaaagcaac tcaggcattg attatttgat tcatattgga attgatacag ttgaattaca
     6361 aggagagcct ttttcgattt gtgttcaaga aaatgatgag gttactcctg aaacctcgtt
     6421 ggttgatgtg gattttgaca aaataataaa atctggtaaa gatacttctg ttatagttgt
     6481 ttttccggag aaaaatcagt tcaatgattt aataatagat aatggtcgaa ttgctcatgg
     6541 gaattcatgt ggaaaaatca ctttttaaaa aagtgtatat aaaaagctat aaaataagct
     6601 aaataggata tttgtcaaat gaagttggtg aacagaaaga actgttttct ttctgttcat
     6661 ttttttattt tattagtaat gattccccat gccaaatcat gatccgtaat ttaaagttat
     6721 aaaagcttcg aaatccattg gcatttcgtt ggaatacctt gatatgatta ttcaaatata
     6781 aaatatagag agaagagaga gagggaagat atttataaag ggcaaagtta tgtttactca
     6841 acttaggaaa tagaaataga tcaatttata ttctcttgtc aatcgaatat gaaccttagc
     6901 actatatacc aaatatagta aaatgctgaa agatgt
//
LOCUS       DAZULY010000146         6899 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000146 DAZULY010000000
VERSION     DAZULY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.147"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            185..1057
                     /locus_tag="ACG81A_002014"
     CDS             185..1057
                     /locus_tag="ACG81A_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5015358.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            1054..3012
                     /gene="nagE"
                     /locus_tag="ACG81A_002015"
     CDS             1054..3012
                     /gene="nagE"
                     /locus_tag="ACG81A_002015"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFS5015359.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(3344..4195)
                     /locus_tag="ACG81A_002016"
     CDS             complement(3344..4195)
                     /locus_tag="ACG81A_002016"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5015360.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            4318..4785
                     /locus_tag="ACG81A_002017"
     CDS             4318..4785
                     /locus_tag="ACG81A_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFS5015361.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(5048..5530)
                     /locus_tag="ACG81A_002018"
     CDS             complement(5048..5530)
                     /locus_tag="ACG81A_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS5015362.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(5630..6493)
                     /locus_tag="ACG81A_002019"
     CDS             complement(5630..6493)
                     /locus_tag="ACG81A_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS5015363.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         2064 a         1420 c         1253 g         2162 t
ORIGIN      
        1 ataactgtct atataaaatt gattacgtta acaataattc gtttcatgtg actagatcat
       61 actaggcatg aaaaatctct gaggaacatg gtttccctag gaggtttttc gtgtctattt
      121 ttgtttggcc gcaatcaact ggtctagcaa acgtgaaaac caaatcttca aaaagggtgc
      181 gcgcatgaaa atcaaaaaga tctttaatca aaatgcagta ttggtagatg acggtggaca
      241 ggaaaaagta gctattggaa aagggattgg atttgacaga aaaagaaatg atttgatttt
      301 tgatcgtgat gtcgaacgga tatttgtcat ggaaccagaa ggtcaaatca agttgcaaaa
      361 tttacttaat cagattgatg aacgcttttt atttgcagca gaaacgatca ttgatcatgc
      421 ggaaactgtt ttgatggaaa agttgaatga acatgtattg atcgctttga ccgaccacat
      481 tgctttttct gcggagaaca ttaataacgg tatcgtcatc cgcaacaaac ttttaagaga
      541 aatcgaagtg ctttacagtg atgaattcag tattgctcaa tgggcagtag agtatctgac
      601 aaaagagctt gggattccat atacttatga cgaagcagga tatatcgcaa tccatattca
      661 cagcggacga agcggtcgga acaataacca tcgcagtatc cgtgaagtga cgattgtatc
      721 tgacatcatc catttagtgg aatcagaatt ggaaacagat atccattcag aaccattttc
      781 actgaattat tcaagactgg tgaatcattt acgactgctt ctacaacgaa cccatgcgca
      841 acaatatgct gttttagaca cggaaatcgt agaaatggtc aaacgaaaat acccagaaag
      901 ctataagatt tctaaaaaaa tccgtgtgtt gctgaccaaa gaataccagc tcgcgatcac
      961 gaaagaagaa ctgggttact tggccatcca tatcgagcgt ttaagaagcg caatcgtaca
     1021 aacaaatgtt aacaatcatg aggaggaaaa aaattgaaag catatctaca acgaatggga
     1081 cgttcccttc aacttccagt tgcggtctta cctgctgctg ctttactagt agggatcgga
     1141 aactggtggg catcttatag taacgacatt gtcgctcatt ttttacaagc tggcggtaac
     1201 gcagttttag gtcaattacc tttgctgttt gccgttgggt tagcactagg gatgtcagtg
     1261 gacaaatcag gtgctgctgc cttggcaggt ttggtcgcat ttgaagttcc agtaaatgtt
     1321 ttaaaaactg attcagtagc tactttattg aatatcaaac cagaatcagt tgatccatct
     1381 tttgctcaga tcagtaacgt atttattggt attattgccg gattgattgc ggcagctctt
     1441 tataatcgtt tccacgaagc aaaattaccg atggctttat ctttctttag cggcaagcgt
     1501 ctagtgccaa tcttggctgc ctttgtcatg ttgctgatca gtgcagtgct actgctcgta
     1561 tggcctccgg tttactctgc tttagtttct tttgctaaat tcattttgag tttaggctgg
     1621 ggtggtgcag gtctttatgg cttcttcaac cgtttactga tccctacagg attgcatcaa
     1681 gcaatgaact ccgtgttctg gtttgacgta gcaggaatca atgatattgg taaatttttg
     1741 gcttctgaag gaacaaaagg cgtaacagga atgtatcaag ctggattttt cccaatcatg
     1801 atgttcgggc ttccagcagg agcatatgct atttacagaa atgcacgtcc agaaaacaaa
     1861 gcaaaaacag cttccttgat gttagcagca gcttttgctt cattctttac tggtgtaaca
     1921 gaaccacttg aattctcatt tatgttcgtt gcttggccat tatatgtctt gcatgcaatt
     1981 ttcactggga tttctttagc agtgagtgca ttcttccatt ggacagcagg atttgccttt
     2041 agtgccggat ttgtcgatta cttcctatct ttgaaaaatc cgattgcgaa tcagccattg
     2101 atgttgatcg tccaaggatt agtaacagca gtgatttact actttggctt taactttgct
     2161 atcaaaaaat tccatctgat gactcctggc cgcgaggaag cagatctttc agatgacgat
     2221 actgcaacga ctaattcaga caataaatat gcggcacaag caagcaaaat ctacgctgca
     2281 ttaggcggag ccgacaatgt aacttcgatc gataactgta ctacacgcct tcgtttgcaa
     2341 gtcaaagata caggcctgat cgatcaaaat aaaatcaaag ccactggcgt acctggtatg
     2401 aagatcatcg acggaaaaaa tgcgcaagtc atcgtaggta cagaagtaca attcgttgca
     2461 gatgagatgg caaaactaca tggtggtgct gcaacacgac ctgcacaaac aaacaccgtg
     2521 gttaaaacag aaacatttgc cggtggaacg atcgaacaag atatttacgc tatcgcaaat
     2581 gggaaactga ttccaatcac tgaagtttca gatgatgtat tcgcggaaaa aatgatgggt
     2641 gacggctatg ctgtattacc agaaaatgga gaaatcttct ctccaatcgc tggtacgatc
     2701 acaaacattt tcccaacgaa acatgctgtc ggaatccaaa cagatgctgg tatcgaagtc
     2761 ttgcttcata tgggcatcaa cactgttgac ttaaaaggcg aaccattcac tttatatgta
     2821 aaagaaggtc aaaaagtcgc aagaggtcaa ttgattgctt tagttgatct tgccgctatt
     2881 caatctgcag gcaaaaacac ggatatggtc gttgtattca caaatggcga caaagtccaa
     2941 agcttagaaa tcgaacccgc acgtgatgta aaagcaaatg ataaaatcgg ctctgtttcc
     3001 aacaaagcct gagccatcgt cttccccaga cttttttatc gataaacaaa caggagctga
     3061 gacaaaaatc ttgtctcagc tcctttttcc gaataaaaac ggcgttctcc tgccaactcc
     3121 gcggtaataa gtccagccaa acgtaacata tgaagagcta ttttttattt gactcttctt
     3181 attacttgat gcagaacagc ttttttacaa cctctgataa acggtgttca aaaaccagtt
     3241 tatcggtatg aagtcaaatt tatgcaaaaa tgtggaaagc cattttcaca aaatttttct
     3301 tcatactcaa aactaatcgg ctttttcaca acctctttat acattattcc tgatccaata
     3361 tttcttttaa tgctgcttca agagaagtta atggttttcc caatactttt tctaaatcat
     3421 cagaggaaat agctaactga tcctttttaa tatcatgttg gatcgctaaa aacatgtcag
     3481 ctgcttcttg agggaatccg gcttctttta gattttcgac aaacgctgca tcacttgctt
     3541 caataatttc taacttttta cctgtcgcac gctctaatgc cttggctaat tcttcatagt
     3601 cagttagatt gccggataat tctaagattt ctggagagtc actttctact agtacttttg
     3661 ctgcagcttc tgcatattca cgtttcaatg cccaacctgt ttttccattt cctgcagcat
     3721 aaacgaattg accagttttt aaagcctgcc caatgatagg taattcattc tctacatacc
     3781 aattattccg taaaaatgta tgggaaatac ctgaatcctt aatcattttt tcagtgaatt
     3841 ggtgatctgg cgctaataca ctgatcgagt gatcggcatc tgcaaaactt gtataagcga
     3901 tataagatac cccagctgct tgtgctgcct tcactacatt cttatgttcg gcttgacgat
     3961 ttcctggaac gccagagaca aaaagcaatc gatctattcc tttcaacgct tctttcattg
     4021 agtctaaatc atcataatca ccaatacgta tattaaatcc agcttctttc agtgcttctc
     4081 ctttttcttc actacgtact agtacataga tttcattttg tggaaccatc ttttttaaga
     4141 attccagcgc ataaccacca aaacctccag ttgctcctgt tactaaataa tgcatatttg
     4201 ccactcccta ttctttttat ttgttaagat ttacagctgt acatttataa attacacctt
     4261 tatcataaag agtgtggtaa gataagtcaa gcaaaaaaaa tgaaaggatg acaactgatg
     4321 aagtattctc tgcaatttag tgatgcaatc catgtacttg cctatattga aatgtttcaa
     4381 gatacccgat cactttctag tgagatgatt gccagtagta tcgaaacaaa tgcagcaaat
     4441 gtacgtaaaa tcatgagtca gctcaaaaaa tccggtttga tctttacaaa aaccggaaaa
     4501 ccgcagcctt cccttgccaa aagtccagat cagatttctt tactagatgt ctatcgcagc
     4561 atcgaaggta atacacagct tatccaagtc gatccaaaaa caaatccaaa ttgcgtcgtt
     4621 ggtgcgaata tccaagaagc tttagaagaa agctacgctc ggctgcagaa aaaggcagaa
     4681 gaagaaatgg ctaagatcac acttgatagt ctgatacata aaatagccgt tttggaaaaa
     4741 gaaaaaagac cagaaaacac aggcctcgta gagagatttt tataattctc tctactgtgc
     4801 ctgcattttt ctgatcggaa tcgtctacat aaattctttt attctccggg ggacaaaagc
     4861 cttgtcatgc ccctttttcc tctctaatgc tcaggtcaaa gcgctgaagt catgacgtct
     4921 tgataaacgg tgttcaagta gctgaccctc ggcattagtt ctgaaaaatg caaaatatgg
     4981 aaagctattt cccatttttc ctctctaatg ctcaggtcaa agcgctactt tcacgacctc
     5041 tatattatca tggtttattg actaccggta tccagatttc tcccatatat ttcccgttgg
     5101 taactcccat ttcgactaca gcatttggac ccccaacata ggcgtaatct tctacttctc
     5161 ttaaaacatg cccaaaggcc gtattcgtca aatcatttct taattcttca ggcgattctg
     5221 ctaaagcgat tacaacaaga tacgttcctt ttgggaatgg gatgattcgg gtagtgtttg
     5281 gaacatcctg actcgtttga acaccgacat agtacatcat tttcccgtta tagggttcgt
     5341 tgactgtaaa gaaataatga ttttcagcag cttgtttcaa tgcatctatt gttccattac
     5401 cgataacttt ttgctcaaat gcttgtttct ctgcagccaa accgacaaaa tccgtattgc
     5461 tactcttcaa ttccacacca atacccgtca ccgtaaaatc tggtttttct tcgatcctat
     5521 actttgccat ttctcccacc ctttctatta tattaagttc atcttacata aaattaaagg
     5581 gaaatgacac gattacgctc ttttttctag ggatattttt attttttaac tatttttttg
     5641 ctttcactgc aatccaaatc tcactgtaaa cattactgaa atcctctccc cattttgtaa
     5701 aagagatctc gggagcttcg actaactcat aatctgaaga aggaagccat tctgcataga
     5761 ttttccccca agtatcttgt aaagtttggg gaaatggccc tttattagga aaaatggccc
     5821 aagtgtgttc ttctatagca agcatttcta aatcagcgta cggattttct tttgtcgtag
     5881 caaagccaat catgtgcatc aaactgccct tttcttccaa gcgggctcca tcaaatccat
     5941 aagaaacgtt gattacttga tttggatata aatctcctaa cttatgcatc tcttcccttt
     6001 gagtctccgt gatcgtctgc gctaactttt gtatttcttg attgactcct tcaaatttga
     6061 caggaacttt tttcgccaca cctgccacat aaaactgttc tttcttttcg atacgaaatt
     6121 ccatcgattt tcctcctctg acatcaataa aaaatgtcaa gcgtggaaaa gatttttgta
     6181 tgccgtatct gctgatttct gaaggcagat atcctgacca atcacgaaaa gctcttgaaa
     6241 aaccttcgac tgtttcatat ccatatctaa acgccgtttc tgtcactctt gatccatcta
     6301 ggagatcgcg attagccaaa gccaatctac ggtttttgat atagtccatt agcgacatcc
     6361 ctgcaataaa cgtaaagatg cgttttaatt gatattcaga gattcctgta ttttcggcta
     6421 ttattttata ggaaagtcct tctgtcaaat gttcttcgat gtaatcgatc aattggttca
     6481 gatctttaag catatttatc ttcctccttg cttgcctttt tatcatatta tcaaagaaag
     6541 tcaattactt gatgaatcag gaaccaattt agtcagatgt agaaaatgtt gtatggatct
     6601 aaagaaaatt ctcaagcagc gtttcgtaac ggataattgg tattatctgc ctatctatgt
     6661 tttaccttta gaaaaataaa ttttggggaa gacacattct aagtaaacaa agcggtaaaa
     6721 atatctttta ttcccttagt taatccgctt actacctact gtacaaagca aattgttgtt
     6781 caaactgctg atcagaacgc tgcttggatt tttctttgcg atacatcata cctcttttat
     6841 caattggaac aattatttat ttctaacaaa atcacttcat ttttgtcaga taaataaaa
//
LOCUS       DAZULY010000147         6792 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000147 DAZULY010000000
VERSION     DAZULY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.148"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..1222
                     /locus_tag="ACG81A_002020"
     CDS             68..1222
                     /locus_tag="ACG81A_002020"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFS5015364.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1244..1996)
                     /locus_tag="ACG81A_002021"
     CDS             complement(1244..1996)
                     /locus_tag="ACG81A_002021"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5015365.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2360..5026)
                     /locus_tag="ACG81A_002022"
     CDS             complement(2360..5026)
                     /locus_tag="ACG81A_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFS5015366.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5367..>6792
                     /gene="cls"
                     /locus_tag="ACG81A_002023"
     CDS             5367..>6792
                     /gene="cls"
                     /locus_tag="ACG81A_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS5015367.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQI"
BASE COUNT         2000 a         1397 c         1239 g         2156 t
ORIGIN      
        1 tttcttttaa tctttttatg ataccttagt aaagtaagtt ttttatcata agtaaagagg
       61 tgttgaaatg aaaataggga ttgatcgtct ttcctttttt attcctaatt tatatttaga
      121 tatgacagag ttggcagaaa gccgtgggga tgatcctgca aaataccata tagggattgg
      181 tcaagaccaa atggcagtca atcgtgcaaa tgaagacatc attacactag gagcaaatgc
      241 tgccagcaaa atcgtaacag aaaaagaccg tgagctaatc gacatggtca tcgtcggaac
      301 agaatccggg attgatcatt caaaagcgag tgcggtaatt atccaccatt tactgaaaat
      361 ccaatctttt gctcgttctt ttgaagtaaa agaggcttgc tacggtggca ccgcagcttt
      421 gcacatggcg aaagaatatg tcaaaaatca tccagaacga aaagtactag tcatagcaag
      481 tgatattgct cgttacggct tggcaagcgg tggtgaagtg acgcaaggtg tcggtgctgt
      541 tgcgatgatg atcactcaaa acccgcgtat tttatcgatt gaagacgaca gcgtatttct
      601 gacagaagat atctatgatt tctggcgtcc agattatagc gaatttcctg ttgttgatgg
      661 tcctttatct aattctacgt atatcgaatc attccaaaaa gtttggaatc gacataaaga
      721 attgtcgggt cgaggactcg aagattatca ggcgattgct ttccatattc cgtatactaa
      781 gatgggaaaa aaggcattgc aaagcgtatt agaccaaaca gacgaagaaa atcaggaacg
      841 tcttatggct cgctatgaag aaagcatccg ttacagccga cgaatcggta atctttacac
      901 tggttcatta tacctggggc taacttctct actggaaaat tcgaaatcac tacagccagg
      961 agatcgcatc ggtctattca gctacggttc tggtgcagta agtgaatttt ttacaggtta
     1021 tctggaagaa aactatcaag aatatctctt cgcacaatct catcaagaga tgctagattc
     1081 tcgaacacgg atcactgttg acgaatatga aacgattttc agtgagacgc ttcctgaaca
     1141 cggagaatgt gcagaatata cttcagatgt accattttcg attacaaaga tcgaaaatga
     1201 tattcgttac tacaaaatat aataaaatca taaaaagaac ccttcacgag tcgtgaaagg
     1261 ttcttttttt atctgcggac caaaggagca gtctataggt cagcttagtc gtactaagga
     1321 tttctcgttt ttcactgaat agtttgcgaa tgtacctggt cacaacagga tcttttgcgt
     1381 acgcgtcata gtatagcttc atcaattctt cagtaattga ttcctgtgta aaataagtaa
     1441 aatctttttt cttcactttg aatccttcat caattaagta tgtttcaaat tgatccatta
     1501 caactggcga tgtattagga tcttcttcta tcattcctag ttgcttttcg ataggataaa
     1561 aaacatcatc gattgattcc actactctga ttatcacgca ccatttgctt gactttttca
     1621 tcaatttttc taattgaata agatgtacca atgctggatt tgctgctgta aaaaccattt
     1681 catatgtctc atttgattgg ataaattctt ctaacgactg tttgataaag gaaacatttg
     1741 gcaactcatt tttttctgct tctttttttg cattttctag cattcctgaa gaaaaatcga
     1801 tcaaatcata gcgattaatt tcttttgcta aggaaagaaa tctgccagat cctccagcaa
     1861 cgtctgctgc tgtttcagca ggcaggactt tctccgtttt cagataattt tttacatctt
     1921 cagcaatagt tacttgcgat tcttgctgga tttcataata ttcttcagca aattcatccc
     1981 attctttttc attcatttga tttcctctaa ttttttgtta tatgttttca cttttcggaa
     2041 taaatcattc tcctctttta tctattctat ctcttttgat gaataataaa atgattggct
     2101 ttccgtctat cataaataaa aaaagggact gtgaccaaag tcgtgtcaaa gtccctcttt
     2161 ccgaataaac agtgtccaat cagatgaccc tcagtattag ctcaaaatgc tgattttaca
     2221 acttcttcct taaacggtgt tctcctgcca actcttcggt cacaagtcac aatattcaac
     2281 aaacatgaga agctgtttgt tgaaattgca ccttgtgact cagaattaaa cggcaggctc
     2341 acaacctcgt tgattcgttt tatttatttc cttgtactcg tacaatgatc aatttagtga
     2401 tttccatcaa aacaattgtc acaagtgcga ttcccataca tgtaaagact tctgtcaatc
     2461 caaatgctga tgggatagag aagacttctt tcagtcctgg gatcattgtt aagcagtaaa
     2521 gcgcgctaca tacaactaca gctagtaaga cacttctgtt agagaagaac cctgcttgga
     2581 tagctgtttg tgtgtttgaa cgtgctggga atgtttgcaa tgtccgacac caaatcaatg
     2641 ttgaaaatga catggctaca ccgaattcag gtgaatgctg catgccaagc cattgagcaa
     2701 agataacagc aatcccaatc agcacaccac gatacgttac agaaatcaat gttttgtccg
     2761 taaagattcc tgaatttgga tctcttggcg ggcggttcat cgtagcaggt tcagcttttt
     2821 ctgtacctaa tgcaattgct ggcagtgaat cattgactaa attgatgaac aacagttgta
     2881 atgctgtaaa tggattggac cagtcagcaa ctaaggcaaa gatgatcgca atgatcgcac
     2941 ctaaatttcc tgcaaataga taagaaatag ctttcttgat attatcaaac acattacgtc
     3001 ctacttctac tgcgctgaca atagaagcaa agttatcatc cgtcaatacc atcgctgctg
     3061 catcttttgc cacatctgta cccgttccca tagcaattcc gatgtctgct tgtttcaatg
     3121 ctggtgcgtc atttacacca tcacctgtca tagctgagat atgaccttta ttttgccaag
     3181 cacgaacgat acgaatttta ttctcaggtg aaacacgtgc ataaacagtg actttttcta
     3241 aaacttcgtt tagttgatct tctgtcaatt catctagttc cgtcccggtc aaagccaggt
     3301 ttccttcttc aaaaatcccg atttgttttg cgatagcaac agctgttgtt ttgtggtcac
     3361 cagtaatcat gattgttttg attcctgcac ttcttgcttc tgttaccgcc tgagtcactt
     3421 cagcacgtgg aggatcgatc atagcaagca aaccaacaag gatcaaatcg tcttcatctt
     3481 caaatgataa atcaaaatta tcaagtggac ggtaagcaaa agctagtaca cgtaatgcgc
     3541 gtgttgaaaa agcttcattt tgctcttgga attttttcat caattcatcc gtcatcggaa
     3601 cgacttcacc atttagcaat acttttttac tgcgttggaa gacgatatct ggtccgcctt
     3661 taacaaacaa gtaatttttg ttatcaattg tgtgtccagt agacattaat tttcgatctg
     3721 agtcaaaagg aagttctgct tgtcttgggt agttgtttcg gatttcctga taaggctggt
     3781 ttaatttttc actaaagtcg ataaacgcta cttccgtagg gtcgcccaat ttagtaccgt
     3841 cttcactgat cgatgcatca ttcgctaaaa cactgatttc gatcaagcga cgttcgtcag
     3901 ctgtccattt tgaagggtca tttgtgaaat ccccagtatc gccattacct aaaaagtagt
     3961 caacaactgt cattttgttt tgtgtcaatg taccagtctt atctgtacag atgatacttg
     4021 tcgaaccaag tgtttcaact gctggaagct tccgaataat ggcatgacgt tttgccattt
     4081 tattcgttcc caaagataac acgattgtta cgatagactg taatgcttct ggaatagcag
     4141 ctacggctac ggctacggca aacatcactg cgctgacgat atctgcagtc atatctcctg
     4201 taccttctcc aagataaata cgaaacagtt ggatacctaa aatcaataaa gacaatgcca
     4261 aaatagccaa acttagtttt ttactgaatt tatccagacc tcgttgtaac ggagtgactt
     4321 gctcagttgt tgattccaaa aggccagcaa cttgtccgat ctccgtatta ttcccagttg
     4381 aagtcactaa gaatttaccg cgtccgtaag ttacgatcgt tccactgaat accatatttg
     4441 tacgatctcc gattggtacc gtacttgtga tatccgttac ttctttttct gcagaagtcg
     4501 actctcctgt cagcatacct tcatctactt ttaatgatcc cgcatctagt aaacgtccat
     4561 cagcaggtac ataatcgcct gcgtctaaca acacgatatc tcctaccaca agttcgtttg
     4621 ccggaatcgt tttttcctta ccatcacgaa tcactcgagc atcgggcgct gaaaggtttt
     4681 tcaacgcttc taaagaacct tctgcttttt ttgtttggac aacactgatt atagaattca
     4741 acatcaatac tgcaaaaata actaagaatt cgatgtagtc acccatgacc atctgaatca
     4801 gtgcgacaat cagcaatacg ataaccattg catctttgaa agtttctaag aacaatttcc
     4861 aagttggctt cttcggcttt tcttttagct ggttcaagcc atctctttct aatttttcct
     4921 tcgcttgcgt ggtgctcaat ccatcggttg agctctgcgt ttgctgcatc aacgagtcga
     4981 tcgtttcttt atagaccgct tgttgtgctt gttccttgtt ttccaaattt catcccccat
     5041 tttttgatct tggccctagt cctgtctgaa aaaaaagaga cctaagacaa atacgcattt
     5101 gtcttaggtc tcaccatttc atacaatacc agaaaaccat ttggttaact tactgttgat
     5161 attatcgcaa caaacgttgc cggttactcc cctaaggaga tattcaactg tactaatatc
     5221 ctaccttatt aacctacaaa aagcaactac tttagcccga tttacctaca ttttcatgaa
     5281 aggagtgaaa agtatattca ccttggtagg cttctttctt ctatcatgct atactaatgt
     5341 tattgtatgt aaggaggaac atttttgtgg tatctagtat tataaccgcc ctttatctat
     5401 taaatgcact tattgctttg gtggctattt tgatcaaacc ccgagatgta gcagccattt
     5461 gggcatggct cttagtattg tttgcccttc ctggggtggg ctttgtatta tatttatttt
     5521 tcggacgtgg attaacggat aagaaaaaat tctatctccg acaaagtgac ttgaaagaat
     5581 tagaaaactt tcagtctttt agaggagata ccattgaaca ttacgatcct gacatgggcg
     5641 ataaagacaa acagcaattt gttgacttct tctcttcatt aaatcgtatg ccgctgacaa
     5701 gaatgaattc tgtcactctt ctcacagacg gacaagagaa attggattca ctgcttcaag
     5761 atctaaaaaa agccaaacat tcgatccata tcgaatatta cgcatttgtg acagataata
     5821 tcggccagca agtcttacat gttttagaag aaaaagccgc agaaggcgtg gaagttcgaa
     5881 tattatatga tgcatttggc tctcatggca caaaagcaaa agatttcaat cgtctaatca
     5941 aaaatggtgg acatgtccat acatttgtta cctcacaaag ggcattactt cgtttccgat
     6001 tgaattacca tgatcaccga aaaatcgttg tgatcgatgg aaagattagt tataccggtg
     6061 gtttcaatat tgccaatcaa tatgtaaata caacaaaaaa attcggctat tggcgcgata
     6121 cgcatatacg gattttcggt gccgcttctt ctttgctcca gcttcgcttc ttaacagact
     6181 ggaacgtctc ggtacctgaa gaaaaaaagg tcggctatca tttgaattat ttctttaaaa
     6241 aagcagatcg agatgaatct aagcttgctg atacatccat ccagcttgtt tcaagcggac
     6301 cgaataacga aagggaacaa atcaagcttt catttatcaa attgattact tctgctaaaa
     6361 aacgtgtttg gatacagaca ccttaccttg ttcctgatga aagtgtcatt gctgctttaa
     6421 aaatcgcaac tgcctctggt gtagatgtga aaatcatgat tcccaacaaa ccggatcatc
     6481 cttttattta tcgagcaaca caatattatg ctcggcagct gatcaaggaa aatgtacaaa
     6541 tccttgtcta tgagaacggc ttcctccatg caaaaacatt gataatggat gatgaaatct
     6601 gcatggtagg ttcagcaaat caagatattc gtagctaccg attgaatttt gaaacaagtg
     6661 ctgtcattta cgatcctgag tttttagaag aacttgctac tcagttcaaa gaagatgaga
     6721 cacattgttc atccatgaca actgaaacag tcaaggaaat gtctaactgg ctattattca
     6781 agcaacaaat tt
//
LOCUS       DAZULY010000148         6674 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000148 DAZULY010000000
VERSION     DAZULY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.149"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..458)
                     /locus_tag="ACG81A_002024"
     CDS             complement(<1..458)
                     /locus_tag="ACG81A_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS5015368.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(471..1802)
                     /locus_tag="ACG81A_002025"
     CDS             complement(471..1802)
                     /locus_tag="ACG81A_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFS5015369.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1813..3255)
                     /locus_tag="ACG81A_002026"
     CDS             complement(1813..3255)
                     /locus_tag="ACG81A_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5015370.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3242..4006)
                     /locus_tag="ACG81A_002027"
     CDS             complement(3242..4006)
                     /locus_tag="ACG81A_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFS5015371.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4110..4985)
                     /locus_tag="ACG81A_002028"
     CDS             complement(4110..4985)
                     /locus_tag="ACG81A_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5015372.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5240..5324)
                     /locus_tag="ACG81A_002029"
     tRNA            complement(5240..5324)
                     /locus_tag="ACG81A_002029"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5288..5290),aa:Leu,seq:caa)
     gene            complement(5342..5413)
                     /locus_tag="ACG81A_002030"
     tRNA            complement(5342..5413)
                     /locus_tag="ACG81A_002030"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5379..5381),aa:Gln,seq:ttg)
     gene            complement(5423..5495)
                     /locus_tag="ACG81A_002031"
     tRNA            complement(5423..5495)
                     /locus_tag="ACG81A_002031"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5460..5462),aa:His,seq:gtg)
     gene            complement(5557..5641)
                     /locus_tag="ACG81A_002032"
     tRNA            complement(5557..5641)
                     /locus_tag="ACG81A_002032"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5605..5607),aa:Leu,seq:caa)
     gene            complement(5662..5732)
                     /locus_tag="ACG81A_002033"
     tRNA            complement(5662..5732)
                     /locus_tag="ACG81A_002033"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5698..5700),aa:Cys,seq:gca)
     gene            complement(5778..5849)
                     /locus_tag="ACG81A_002034"
     tRNA            complement(5778..5849)
                     /locus_tag="ACG81A_002034"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5815..5817),aa:Gln,seq:ttg)
     gene            complement(5861..5933)
                     /locus_tag="ACG81A_002035"
     tRNA            complement(5861..5933)
                     /locus_tag="ACG81A_002035"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5898..5900),aa:His,seq:gtg)
     gene            complement(5936..6009)
                     /locus_tag="ACG81A_002036"
     tRNA            complement(5936..6009)
                     /locus_tag="ACG81A_002036"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5975..5977),aa:Trp,seq:cca)
     gene            complement(6016..6096)
                     /locus_tag="ACG81A_002037"
     tRNA            complement(6016..6096)
                     /locus_tag="ACG81A_002037"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6060..6062),aa:Tyr,seq:gta)
     gene            complement(6098..6173)
                     /locus_tag="ACG81A_002038"
     tRNA            complement(6098..6173)
                     /locus_tag="ACG81A_002038"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6138..6140),aa:Phe,seq:gaa)
     gene            complement(6209..6281)
                     /locus_tag="ACG81A_002039"
     tRNA            complement(6209..6281)
                     /locus_tag="ACG81A_002039"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6246..6248),aa:Asp,seq:gtc)
     gene            complement(6291..6363)
                     /locus_tag="ACG81A_002040"
     tRNA            complement(6291..6363)
                     /locus_tag="ACG81A_002040"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6328..6330),aa:Val,seq:tac)
     gene            complement(6379..6450)
                     /locus_tag="ACG81A_002041"
     tRNA            complement(6379..6450)
                     /locus_tag="ACG81A_002041"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6415..6417),aa:Glu,seq:ttc)
     gene            complement(6465..6554)
                     /locus_tag="ACG81A_002042"
     tRNA            complement(6465..6554)
                     /locus_tag="ACG81A_002042"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6518..6520),aa:Ser,seq:gga)
     gene            complement(6594..6667)
                     /locus_tag="ACG81A_002043"
     tRNA            complement(6594..6667)
                     /locus_tag="ACG81A_002043"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6631..6633),aa:Asn,seq:gtt)
BASE COUNT         2002 a         1479 c         1098 g         2095 t
ORIGIN      
        1 taaaattcaa aacaactatc tttaatcttt acttcttggt tttgttttaa taaatttcct
       61 tcaaaaatgc ctggagaaac tacaatgatc accgtatctt tttgtgcttg ctccaacttc
      121 ttcaaatcta taatagctaa cagtgtccct tcttttacct cctggttact ttcaacccgt
      181 atctgaaacg gacttccttc gagttcaact gtatctaaac ccatatggat cagtattttt
      241 ctgccagatt cactttcgat cgttatggca tgtcgggtag gaaatatatt atcgattatt
      301 cctttaactg gcgcatggat atatccatca tgattcgtta tagcgtatcc aactcctatc
      361 atttttgaag aaaagacctc gtcttttact gctgttaatg gaataatttg accactagct
      421 acagctttta gaatgtttct tgttttaaag aaatgcactt tttatcacct ctactttaat
      481 gctggccaat attttttgtt tactttgatc atttcatcta gtattttttt tgcgactctg
      541 gcatttggta cggtttgatt caatgtaaat gcttgtaaag ctttttgata agaaccttca
      601 aagtaagcat ctactagtaa cttctctact gcaacttgtg cctccatcaa tcctttatga
      661 aaatcactga ttggttttct tagtgtaatt ggctccacac cagttgcacc aacatatgcc
      721 ggtatttcaa caactgcatc tgaccgtaga ttcggaatcg ccccttgatt aggaacgatt
      781 agcataaagc gacgacgatc atcattcaac aaggaaatag ctatatcgac gatatattgt
      841 ccatgctccc caaaattaaa gttcaatact tcttccacaa taccgcggcg tattttatca
      901 gccatttctt tggtattttt ctctcgtcca tccatgacca tattagcacg tgtatactct
      961 ttgtcagtat gctcaacgac catatcagga tataagtaat actctaagta attattcggg
     1021 atattacttg gaaaattttt cgtgatgact gacatcattt gaaaggtttt ttgccaagtt
     1081 ttatctccta tattaaaatc ggcgacttgc atttcttttg ttattagttt ttcaataatc
     1141 tctggcataa tttctcgttg caattcttta tcataaatcg atgtatacca gccaaagtgg
     1201 tttaaaccat aatacgtaac gatccaattc ttacgatcat aaccgtaatt cactgcaatc
     1261 gtttcttcaa ttgaaatagt catatcacag gcattaatca tttttacatt cggatattta
     1321 cgacgaacag cttcagaaac gatcgactct ggatttgtat aattcaaaat ccatgctttg
     1381 ggggcatagt cttgtatatg atcgacaatt tccaataaac ctttgattga gcgcatccca
     1441 taagcaaatc ctccgagtcc acacgtttct tgtcctacta atccatgttt aagtggaatc
     1501 ttttcgtcct tttctcgcat ctccaatcct ccaacacgaa tttgtgagaa aacaaaatcg
     1561 catcctgtaa aagcaagctt tggatcatcc gttgcacgaa tgcgaatatt tgaaaaacct
     1621 tttcttttca gcataaattt aacaatcaag tacatatcca tatttctttc ttcatcaata
     1681 tcatataaac gtatttcaga taatggcaac cgtttttcat tgttcaaaat ggcttgaacg
     1741 attcctggtg tatacgtact cccgcctcca gctatagtga taatctgttt tttcatcatc
     1801 atcttttcct ttctattctt ctaataattt attgaattct gccataacat aagtcacttc
     1861 taagccgatg ataatttgat aatttttttc agttggttta atgatcccgc ttatccctaa
     1921 ttgcttgatt cgctgttcct cgaccatggt gggatccact acctctaccc gtaggcgtgt
     1981 attacaacta tagacatcca ggatattggc ttttcctcca aacgcttgta gcatttttgt
     2041 tgccatatat tcataatttt tacttgctaa ccctaactct gtttcttcta aggttacttc
     2101 attgccaaac tctgccttag cttcacgtcc taatgtctga atgtttctct tattgatcaa
     2161 ataataaaaa gtaacgtaat acaaggcaaa aaagaaaatt ccgattggaa gaatcaaaat
     2221 agcattggta gcgattcgaa aattgatgag ataatcaaca atacatgctc cgaatgtaaa
     2281 accaattcgt atattgaata agtaacagac aatccctgct aaaccggcaa aaagtgcatg
     2341 gatcacgtac aacataggag aaagaaacat gaaactaaat tcaacaggct cagtgatatt
     2401 tgcaataaat gaagttcctg caccgcttcc catgattcct ttgaccatct ctttattttc
     2461 tttataagct gctttataga tagctagaca tatagctggt ataccaaaca tcattactac
     2521 aaaaaaaact gataaaaaca gtccagcctg aggatctccg ttgataaagc gtgtcatctc
     2581 tcctctgaaa acttccttgc taggagatgt atagcttcct aaatcgtaat aaatatacgt
     2641 attcaacaca tggtgcagtc caaaggggat cagcaaacga ttcaaaaaca taaatatccc
     2701 cacacctaat gctcccatat caacgagcag ttttgcaaaa ttatcaatcc caatttggat
     2761 gagcggccag ataaagccca aaaaaacagc tgcgattgtt tgtagaatca tcaccatagt
     2821 caatgggaat ttttcgcccg caaaataaga aaaaactact ggtaattttt gttctttaaa
     2881 gcgattatac gtccatgccg ctactaaacc ggaaataatt ccaccaaaaa tccccatatt
     2941 cacttctggg tccataattt ctaagccttt tttcaaggta gcaatcgcta aaaaaccagt
     3001 aagtgcaggt attcctttgt ccttattttt gacaaaaccg attgcaactc ctatagcaaa
     3061 taagatatcc aagtttccaa aaacggcatt tccagctgca ccaattactg ggatgttcag
     3121 catatcgtca gctgataaac gcatgatcaa tcccgctaat ggcatcgctg caactggaag
     3181 tagcatcgct cttccaagct tctgcacatt tgatttaaca ttcattttta ttcctcctct
     3241 attaaatatc ctcatatgtt actaactcaa tcaaaggatc atttttcact ttttgagcaa
     3301 atgctgaatg aattaaaaat tcgacttggt gggtacgagg atacacaaaa cttgaaccat
     3361 ttaacaattc ctgatccaaa tacgctgcgt gacacatgac ttcgatcgtt ttatcttctc
     3421 tcaatatcat gagtaaactt gccaaatatt cttccacact cgtattgatt tgtcgttttt
     3481 tttcatcagt ttctcctacg acatcgaaac aaggctcaaa ataatcgaca tgacgaactt
     3541 catcttttcg ttcaaagttg ctccgaaccg gcaaatcata tttttttgct aatgtaacaa
     3601 ctacctcctg attttcatcc aaagtatgag tatgatgatg actgtctaaa tgagttggta
     3661 tgatccccgc atgataaatt ttttgaatct gagcattcca ttcctgatac agttgatctt
     3721 tgtcaatgtt aaatggatgc tgataataag ataaggatct gaactctccg ctttcgataa
     3781 gagtatcgac agttttgagg agtggtttgc cacacgtcaa tgttaaatgg actcctacac
     3841 ctagcctagg catttccttt cttagtttca cagcatgctc gaaacccggc atatttgcca
     3901 tcaatgtagt tgatgtcaaa attcctcgct gataagcatc cataatccca taattcacac
     3961 catggctata gccaaaatca tccgaattaa aaatcacttt tcccatttca ttctccttct
     4021 tttttctact aaaataggcg caaccaaata atcatagtcc atttactatg tgtcatttgg
     4081 ttgcgcctat agatagttac gcccttattt taatagattc ttgctagatg aattgccaaa
     4141 tagatgattt cattttcttg aatggcaagt tgaaattgct gttcgatgat atctttaatc
     4201 tttagtgctg tacaatacgc tttgctatgt ttaaatgttt cagtaaaaaa accaatattt
     4261 tctactggcg tttcttcctg ataaatcaag cgtttaacca ggaatttgat atgaaccatt
     4321 aagcgatgat aagtcagtga ttctttatca taaacgtttg aataggattt ttctagatgg
     4381 gacaaaatcg tggtcgtcat ttctactatt tttaagctct ttaaactatt aggctctcct
     4441 agctcagcat taatgatatg catagtgatg aatcctactt catttgaccc taactcggtt
     4501 tgatatcgat gattgattcg ctcgaccact tctactgcta tatcatattc atttggataa
     4561 aggaatctca tttctggcaa cataattgct gtgacatctc tatcttctct ttttcgttga
     4621 atagcaaaat aaatatgatc tgtcaatgta atatagatat ttgaatctaa tggagaggag
     4681 atcctagctg ttatcagttc aatcacttct tccgcaaaat ccataaattc gattggaatc
     4741 tgcgagacta tctcactgaa tcgtttgatc ttttctttag aatctaatac aaataatttg
     4801 tcagcatcct caacatcaaa tggcttactc actttagcag aaaagccaat ccctttcccc
     4861 atcgcaattt tttctattcc atcgtcatcg atcaatacag cattatgatt cagctttttc
     4921 atcacataca ccgttaattc acctcctcta tatgataaaa caaaacaact tgcagactcc
     4981 ttcatacata tttttgtctc caaacaattt ttaaagacaa aaaacgacct aagaaagaac
     5041 cctttcttag gtcgtgccta attgaatagt agcaccctac ataatttaca aaaatagttt
     5101 aacacataat aaaagcgtta tcaatatctt ttttactcgt tttatgataa tacttgtatc
     5161 gaattgattt ggaggtaatt acataaaaac aaaaaaaata tagcaactct ctataagaaa
     5221 gttgctatat tgataattat accggcggcc ggggtcgaac cggcacgtcc tcaacggaca
     5281 ctggattttg agtccagcgc gtctgccaat tccgccacgc cggcaaattt aaaaataata
     5341 actggggtag ctggattcga accaacgcat gagggagtca aagtcccttg ccttaccgct
     5401 tggctatacc ccaagaataa agggcgaatg atgggaatcg aacccacgaa tgccggagcc
     5461 acaatccggt gcgttaacca cttcgccaca ttcgccatca taaaagatgt tatttaattt
     5521 ctcaataaaa aactaagatg aagacatctt agtcaatacc ggcggccggg gtcgaaccgg
     5581 cacgccctca acgggcactg gattttgagt ccagcgcgtc tgccaattcc gccacgccgg
     5641 caagttgatt tattagtact aaggcggtaa ccggatttga accggtgatg aaggttttgc
     5701 agacctctgc cttaccactt ggctataccg ccattatact attatcaatc tataaaataa
     5761 agaataaatt atagaaactg gggtagctgg attcgaacca acgcatgagg gagtcaaagt
     5821 cccttgcctt accgcttggc tataccccaa gaataaaaag ggcgaatgat gggaatcgaa
     5881 cccacgaatg ccggagccac aatccggtgc gttaaccact tcgccacatt cgccatggca
     5941 ggggcagcag gaattgaacc cacactaacg gttttggaga ccgctgttct acctttaaac
     6001 tatgccccta taaaatggag gagagtggat tcgaaccact gaaccctaag gaacggattt
     6061 acagtccgtc gcgtttagcc acttcgctac tcctccatgg tggcgcggga cagaatcgaa
     6121 ctgccgacac acggagcttc aatccgttgc tctaccaact gagctaccgc gccatatttt
     6181 aaaattatta attgaataac aagaacgacg gttccgacgg gaatcgaacc cgcgatctcc
     6241 tgcgtgacag gcaggcatgt taacccctac accacggaac cagttcatta tggaggttaa
     6301 cgggatcgaa ccgctgaccc tctgcttgta aggcagatgc tctcccagct gagctaaacc
     6361 tccaacattc aattaaaatg acccgtacgg gattcgaacc cgtgttaccg ccgtgaaagg
     6421 gcggtgtctt aaccgcttga ccaacgggcc aggtaaattt gctacggaga gtaagggatt
     6481 cgaacccttg agacagtttg gcaccatcta catgatttcc aatcatgctc cttcggcctc
     6541 tcggacaact ctccagagtg aaagaagctc aatactcttt ctcaaaatga gaactccggc
     6601 agtaggactc gaacctacga catcatgatt aacagtcatg cgctactacc aactgagcta
     6661 tgccggaata acat
//
LOCUS       DAZULY010000149         6570 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000149 DAZULY010000000
VERSION     DAZULY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.150"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACG81A_002044"
     CDS             127..579
                     /locus_tag="ACG81A_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015373.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACG81A_002045"
     CDS             593..1282
                     /locus_tag="ACG81A_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015374.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACG81A_002046"
     CDS             1407..3758
                     /locus_tag="ACG81A_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFS5015375.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="ACG81A_002047"
     CDS             3838..4233
                     /locus_tag="ACG81A_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015376.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="ACG81A_002048"
     CDS             4558..5175
                     /locus_tag="ACG81A_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5015377.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="ACG81A_002049"
     CDS             5216..5515
                     /locus_tag="ACG81A_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFS5015378.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="ACG81A_002050"
     CDS             5549..5716
                     /locus_tag="ACG81A_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS5015379.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="ACG81A_002051"
     CDS             complement(5761..6366)
                     /locus_tag="ACG81A_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5015380.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2265 a          910 c         1285 g         2110 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaattttt
//
LOCUS       DAZULY010000150         6544 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000150 DAZULY010000000
VERSION     DAZULY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.151"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            265..2031
                     /locus_tag="ACG81A_002052"
     CDS             265..2031
                     /locus_tag="ACG81A_002052"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFS5015381.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2133..3392
                     /locus_tag="ACG81A_002053"
     CDS             2133..3392
                     /locus_tag="ACG81A_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS5015382.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3539..4840
                     /locus_tag="ACG81A_002054"
     CDS             3539..4840
                     /locus_tag="ACG81A_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5015383.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4843..5694
                     /locus_tag="ACG81A_002055"
     CDS             4843..5694
                     /locus_tag="ACG81A_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFS5015384.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5707..6522
                     /locus_tag="ACG81A_002056"
     CDS             5707..6522
                     /locus_tag="ACG81A_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFS5015385.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
BASE COUNT         2089 a         1146 c         1325 g         1984 t
ORIGIN      
        1 atatacttct acgccaaaaa ctgttaaaaa acttttactt tagaaaattg gagaaattga
       61 tcgcttaaga taaattcagg acactaaata gtacaggaag tagactaaaa cttttaaaag
      121 ttttaagtct atctatttgg caagtttaaa aaataaaaat tcggtatcaa aaaactgtta
      181 ccgataacat ttttgaaagc gttgtctttt gcttctctat cacttatagt gatgacgtca
      241 tattaaaaca caaggaggaa atcaatggat acagctgcaa tttatcatcg accggaaagt
      301 gaatatgcat ttctatatac gaatacaaga tttcgtattc ggcttcgtac taggaaaaat
      361 gatatcaaaa aagtatatgt gctatgtgga gatccatata ccataacaac tgaaaaatgg
      421 tatcaaaaac aacgaccaat gaaaaaatgt ctaagtacca atgttcatga ttattgggaa
      481 attgaagtga cgagcgaaac aagtcgctta cagtatgcgt tccatgttgt tggggaagat
      541 aacacggact gtttttacgg tgatcaagga atttttcctt atcgagaaaa tgtgattact
      601 gaaccaaatt tttattttcg aataccttat tttcatcaaa tcgatcgatt cactattcca
      661 gaatgggtaa aagaaacagt atggtatcaa atttttcccg aaagatttgc aaacggggac
      721 aaagcaaatg accccgaaca cactttacct tggggaagta aagatccaga ccgagaagac
      781 tttttcgggg gagatttgca aggtgtgatg gatcatttgg attacttgac ggaccttggc
      841 atcaatggca tctatttttg tcccatattt aaagccacat ccaatcataa atacgatacg
      901 attgattatt atgaggtgga tcccgctttt ggcgataaaa aacttttaaa aaacttgatt
      961 gatgaagcac ataaaagagg gatacgtatc atgttcgatg ctgtattcaa tcatatgggt
     1021 gtccattcac cacagtggca agacgttctc aaaaacggag aaaaatcgat ctataaagac
     1081 tggttccaca tccgtttttt cccagtagat tcttatcaga tgactgactt accagaaact
     1141 gctgaaaata tcccatatga tacttttgct tttactccat tcatgccgaa actaaataca
     1201 gcaaatccag aagtgcaaaa atatttgctg gatatcgcaa cttattggat aaaagaattc
     1261 gatattgatg gatggcgtct tgatgttgct aatgaagtcg atcatcattt ttggaaaaaa
     1321 ttcagagaag cagttacaga aatcaaacca gacatctata tccttggaga gatttggcat
     1381 tcctctcaag cttggctgca aggggatgaa ttccatgcag tgatgaatta tgcatttaca
     1441 gattcgatta aagattattt tgcaaaaaag aaaatcacag cttcacaaat ggtcagtgga
     1501 atgaatcatc agcaaatgct ttacagagat caggtgaacg aaggaacatt caacttatta
     1561 gattctcatg atactgccag gatcttgacc ctttgccaag gtaacaaaga attgatgaag
     1621 tctgtgatgg cctttatgtt tcttcaaaaa gggtctcctt gtatctatta cgggacagaa
     1681 atagggatga ctggtgaaga tgacccggat tgccgcaaat gtatgatatg ggagaaggag
     1741 gaacaagatc tagagctatt cggttttata aaagaacttg tttctctaag gaaacagttg
     1801 tctaagatta tttctgaagg ttctactcag tggctaatag taaatgatcg tgaagataag
     1861 ctctatttca cacggaaact agagggacag atcatctatg tttactttaa tcagtcaaaa
     1921 gaaccttggg tagtagaaca ggaaaatgag gtgattctta gtcaaaattg tcagttgtta
     1981 gaagacggga aagcagagat acgacaatac ggatttttga tcatgtgttg aaaactgtta
     2041 ccggtaacaa caagaaagcg tttgcaaatg aagatttgtg cccattataa taagggtgta
     2101 acagcacgtt gaatacaaag gaggaaatga agatgaaaaa aagaagtgtg aaacagttgg
     2161 gtttagggtt aatctcttta ggaatcttag gcggattagc ggcttgtggc aatggtaact
     2221 cttcagcaga aggggaagcc gatgaaggaa aaacgttaac agtttccgtt gattctggat
     2281 ataaagatta tataaacgaa ataaaaaaca ctttcgaaaa agaaaacgat gtgaaaatca
     2341 agctagtaga aaaggacatg ttcgatcagt tggaatcatt agcgttagat gggcctgctg
     2401 gaaagggacc tgacgttatg atggcagcat atgaccggat cggtgcgtta ggacagcaag
     2461 gacatctggc ggaagtaaaa ctgggaaatg aagcagctta cgatgaaaca gacaaagccc
     2521 aagtgactta tgatggaaaa atctacggag aacctgcagt aatcgagacc cttgttttgt
     2581 actataacaa agatttagtc gatacagcac catcaacgtt taaagatttg gagaatctat
     2641 ccaaagacag tcgttttgct tttgagtcag aagcaggaaa aaatacagga tttttagcta
     2701 aatggactga cttttattat tcctatggtt taattgcagg ttacggtggc tatgtatttg
     2761 gtgatgatgg cacaaatcct tcagatatcg gtttgaataa tgcaggtgct gtagaaggaa
     2821 tctcttatgc aacagattgg ttcaaaaatg tttggccaaa gggaatgcaa gacataaaaa
     2881 gtgcaggaga cttcgccagc cagcaattca tgtccaataa aactgcagca atcattgacg
     2941 gtccatggca agcgcaaaca tacaaagaaa acaatatcaa ttatggtgta gccaaaattc
     3001 ctactctaaa taatggacag ccttatcaac cgtttggcgg aggaaaagga tgggtagtca
     3061 gcaattatgc taaaaacaaa gacctctctc aaaaatggct tgattatgta acgaatcagg
     3121 aaaatcaaga aaaattcttt gaaatggtaa atgaaatccc tgcaaaccaa caagctaggg
     3181 aaacagcaaa ggctaaaaac gatgaattaa caacagctgt gattgaacaa tatgaaacag
     3241 ctcaagcgat gccaaacatc ccagaaatgg gcgaagtttg gacaggtgca gaaaacttga
     3301 tgttcgatgc agcatcaggt tcaaaaacac caaaagaatc cgcagatgaa gcagtaaaaa
     3361 caatcagtga agccatagaa caaaaatact gattaaaatg ataaccgtcc atttttataa
     3421 aaaatggaac aggtgcttca gataaaagtg gcaagagcga gatggaaaat tatcttgctc
     3481 ttgttgccat tatcttaaaa gcagtttgcg gtttagcaag tttagagggg gcattcagat
     3541 gcagcaagat aaaacaagca atatcaacga aaaaaaaatc acgctttgtt cacttattcc
     3601 agggatgggg cagtttgtaa atggccaagc ttttaaaggt atcgtttttt tcgccatatt
     3661 cgtcctattc attggtcaga tgcttctggg cggatttcat gcattggtag gattggttac
     3721 tttgggaagt ataccaatgg aagatcattc attatttatt ttgattgaag gcactttgca
     3781 attcatcatt acaatcattt tcttgatttt ttacggtttg aatcttttag acgcttatcg
     3841 agtagcgaag atgcgtcaag agggcaaaaa aatcaatcgg acgatgaagg aagtcataca
     3901 taatataggt gatgaaggtt ttccttatct attgacactt cccgcttatc ttctcatgat
     3961 ttttacgatc attttcccag tcctagttac attgttcact gcatttacta attatgactt
     4021 caatcatatt ccaccagcca gtttaattga ttgggtaggg gctaaaaact tttttagtat
     4081 cttcttttta agctcatatc gtaatacttt ttttgcagtc ttttcgtgga ccgtgatttg
     4141 gactttatgt gcaacaactt tgcaaatcac ccttggtatt ctcacagcgg ttgttgctca
     4201 ccagaaattt attaaattca aacgtatttt cggtatcatt ttcttattac catgggcggt
     4261 cccagctttt attactatca tgagtttttc gaatatattc aacgatagcg taggtgccat
     4321 caatactcaa gtgattcctt tcataaacca gcttcctttt gtagatatca gtgcaatctc
     4381 ttggaaaacg gacccgaact ggacgaaagt agcaattatc ttagtgcagg gctggttagg
     4441 atttccctat atctatgtga tggttacagg aatcttacaa tcgatttcag aagacttata
     4501 tgaagcagca aaaatcgacg gagcaaatgc atggcagcgc ttttcaaaca tcacgcttcc
     4561 tgctgtcttc ctagtggcag cgccaacatt tattacacag tatacgggga atttcaataa
     4621 tttctccatg atctatctgt ttaacaatgg cggtcccgga agcgtaggcg gaggagctgg
     4681 ttctactgat attcttattt cctggatcta taaacttacg actggaactt ctccgcagta
     4741 ttctatggct tcagcaatca cgttgattat ctccgtcatc gttatctgtg tgtcattgct
     4801 ggtatttaaa aagacaaatg cgttcaatat gggggattaa caatgaaaaa atttcattcg
     4861 agtgctcgat tccaaaaacg actttctcac ttttttactt acttttttct tttaatactg
     4921 tcagtcatta tcatctatcc gcttttgatc acagcaagta ctgccttcaa gtcaggaaat
     4981 attgcagctt ttagtttaga tctttcaagt gactggactt taggaaactt tactcggcta
     5041 ttcagtgaaa cactttacgg gacttggtat aaaaatacat tgatcatttc tgtcctaaca
     5101 atgatcgtac aagtgactgt agtaactttg gcgggctata cttatagtcg ctatcgtttt
     5161 atcggccgca aatcaagttt gatgttcttt ttagtgattc agatggtacc gactatggca
     5221 gcattgactg ccttttatgt catggctttc ttattagaag cgctcgataa gtactggttc
     5281 ttgacaatga tctatatagg cggtggaatt ccaatgaata cttggctgat gaaaggatac
     5341 tttgacactg taccaataga tttagatgaa tctgctaaac ttgatggggc agggcatttc
     5401 aggatttttt tccagattgt attaccgctt gtcaaaccga tgctcgccgt tcaagcatta
     5461 tgggctttta tgggaccttt tggggatttc atgttagcta agttcctgct tcgaacacct
     5521 gaaaatcaga ctgttgctgt cggcttacaa acatttattt ctgatgtgac aaaccaaaga
     5581 gtctctcttt ttgctgctgg ggcaattttg attgcactac cgatttgtct attattcttc
     5641 tttttacaaa agaactttgt taccggcctg acatctggcg gaacaaaagg ataggagcag
     5701 agtatcatgc aaacatttcc gttaaactat ttttccgcat tacgtacgcc aactcaagtg
     5761 tttgcgggac gcaaactatt atcatggcct aaatttttct tgatatttgt atttttagtt
     5821 tccttgatgg tcatgcctgt gactcttttt tatgccaatc agatccaagc gattccatta
     5881 gaacaatttc tatccgttca ttcgctgatc gatgaacaag gaacccaaaa attttctgaa
     5941 cttgaattat ctgaaactgg gttacaagca tcgcaacaaa cgatagctgt gacccctgaa
     6001 attttggtag gtgtgtcttt atctgagaaa caacaatccg atcatggaac ttttattgat
     6061 tttgagaagg aacaatgggt gatccaacaa aaggataaat ctggtatacg ccgctatacg
     6121 atgaattata gcccatcttt ccaaccagat tctgtacgta cgcccgaaga tttccaacga
     6181 tttttagaaa gagaatttta tgccagtaat cgtccaacaa tcattttatc ttatagttta
     6241 agtcttggtc ttgtcctatt tgtcatgacg agtctcattt tattcggggc ttcctttttc
     6301 ttgtggatga caagaaaaag ccaactttca tccattcata cattcaaaga gagtgcaaat
     6361 ttgatgctga atgttatggg aataggttcc atggcagcag cagttgtagg gtttatccac
     6421 tttgacttta tcttgatgct aagtgtccaa acaacgataa ctgtcttgct gcttttatgg
     6481 gtttttgtca aaacaagatt caaagataag cgtgtcgaat gacaagttta agcgatttat
     6541 gcga
//
LOCUS       DAZULY010000151         6543 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000151 DAZULY010000000
VERSION     DAZULY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.152"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..1012
                     /locus_tag="ACG81A_002057"
     CDS             2..1012
                     /locus_tag="ACG81A_002057"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5015386.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1081..2025)
                     /locus_tag="ACG81A_002058"
     CDS             complement(1081..2025)
                     /locus_tag="ACG81A_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5015387.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(2009..4729)
                     /locus_tag="ACG81A_002059"
     CDS             complement(2009..4729)
                     /locus_tag="ACG81A_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5015388.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(4726..5946)
                     /locus_tag="ACG81A_002060"
     CDS             complement(4726..5946)
                     /locus_tag="ACG81A_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFS5015389.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(6227..>6543)
                     /locus_tag="ACG81A_002061"
     CDS             complement(6227..>6543)
                     /locus_tag="ACG81A_002061"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5015390.1"
                     /translation="NIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNL
                     KKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERIL
                     TI"
BASE COUNT         1911 a         1261 c         1069 g         2302 t
ORIGIN      
        1 aatgaaaaaa aaatcaatta ttttggcagc tactagtgcg ttggctgttt taactctagc
       61 agcttgttca ggcgatacta ataaagatat cgcaacgatg aaaggcggaa cgatcactgt
      121 atctgatttc tatgatgaag caaaacttga atcatcgaac caatccttag tacaacggat
      181 gatcatttat aaagtattca ataacaaata tggcgataaa gtaaccgata agcaagtgga
      241 tgcagaatac gacaaacaag cgaaatcatt aggtgatact tttgaatcac aattagaagc
      301 tgctggatat acgaaagata cttataaaga atatattcgt aacaatttag cttttgaagc
      361 tggcttgaaa gctcacgtag atattacaga tgacgatttg aaaactgctt ggaaatcttt
      421 ccatccagaa gtagaagcac aaatcatcaa attatcaagt gaagatgaag caaaagatgt
      481 gaaaaaatca gcggatgatg gagatgattt ctctaaatta gccaaagaca aatcaactga
      541 tacagagaca aaagaagatg gcggtaaagt taaattcgac tcaacgacaa caactattcc
      601 tgcagaagta aaagaagcag ctttcaaatt gaaagacggt gagatctccg atgttattac
      661 tacaacaaat cctacttctt atgcgacaga atactacgtt gtcaaaatgg tgaaaaacca
      721 aaataaaggc aatgacatgg ataaatacaa agatcaacta aaagatatcg ctactgaaac
      781 taaactaagt gacaatgcat tcacaactaa agtaatcggt gaagaattaa aagatgcaaa
      841 tgtaaaaatc aaagatgatg cctttgaaaa tgtgttgagt gcattcacaa caacttcatc
      901 aagtacgaaa gactcaagtg aaactacagc ttcaacaaag agttctgaca caaaatcaac
      961 tgattcaaca aaagaatctt caactgaaga aacaactgat tcatcaaaat aaaagaaaac
     1021 aaaaaaagcg aagacttatg tctcgctttt tttgttttga atgaaaaaaa taaatttttg
     1081 ctataaatct tttggtacat agaaacttcg gttgtccata ccgaaaatcc gatcggtgta
     1141 ttttcctggt tctgtttgac gaatcgaagt caacatcatc tgcatcgaag catcgatatt
     1201 gtcgatttga tgtaatattt ctgcttccat tattcttgga cgtacaggtg agccatattc
     1261 taataagcca tggtgtgcta aaaccatatg acgtaataca agaacatctt cgtcagcttc
     1321 gctgattttc aacgcaagac aggctttagt tatttcttcg tcaatcaata ccagatgacc
     1381 gatcaaattt cctgcaagcg tatattctgt cgacatagga ccggaaagtt caatcacctt
     1441 tcctaaatca tgcaaaatga taccagcata caacaatgct cgattcagct caggatattc
     1501 atcggcgatt gatttcccta agtgaagcat tgtcaaagta tgataagcta atccactggc
     1561 aaatgcatga tggtttcttt ttgctgccgg aaactcaaaa aattgttttt gatatttatt
     1621 aagtagatag cgaacaatac gattccaatg cgcattggtg atctcaaaca acaattgatt
     1681 gatttcttct tccatttctt ctttcttcaa aggggctctt tccatgtata aactaggctc
     1741 tcctggctca tctacccttg ttaagcgcat atgaagaatc tttacttgag ggtttccttg
     1801 ataaacctct cttttaccgt ttagaaaaac gactgtgccg gctgtaaatt tattgatttc
     1861 ctcctccgaa gcatcccagt atttaccatc gatagtacct gaagtatctt ggaaagtaaa
     1921 agcaataaat ttttttccgt ttttagcgat tcttacatct gcacttttga tcaggacaaa
     1981 ttgttcaaac tgctcatcga ttactaaatc acgaattttt ttcattattt gctccttcta
     2041 attgttttac gatttggttc atctcatggt aatagcttac catttcttta tcagaagaaa
     2101 aacaaattat ttgatacttt tctccaaatt tagctaaaag ttcagctaat tgatactttc
     2161 gctgatgatc ataatgaagc cagccatcat caatgatgat cgggcatatc gaacggttag
     2221 cttctaatga gaggtaagca aatctcatcg ccataatcag ctgatctttc gtacctgtag
     2281 ataactcata gatgggaaat tctgtatatc cgtctgttaa agtcagtgta tcatttttca
     2341 gtaatatctt ctgataattt ccttttgtta aaatacctaa ataatcacct gctgctctta
     2401 acaactgggg taattgtctt tcactcatct ctgttgaaag atcacctaag aatgtcccgg
     2461 cgatttcaag tgctccccat tcctccagta attcctgtaa ttttgctttt tgccggctag
     2521 cttgttgata aagttcgtcc aatgtgccgt ctctttgcaa ctcttctact tgaagttgga
     2581 gtcgttgccg attttcactt gcttcttttt cttcttgctg gagttgggat tgcttttctt
     2641 tcagttggcg cagtcttaca gataatttct cttttgtcag ctcttcagga aatagtggag
     2701 taagcatttc cgataattca ttgtatcttt tcatctgctg ttcggcttgt ttggcttttt
     2761 gtaaaaagac aggtacttcc gaaggataag ataagcctgc ttgagtcaat tgttcgctgt
     2821 attcttgtat ccatactttt tgtttctttt tcaattgatt gatttcttgc ttcaataaag
     2881 tattttgctg atagcttctg gcaaatttca tctgttccat ctcttgtata aaacgggaaa
     2941 gcaataccat tttttctgaa attgtcttat cttgtatcgg taaccattct ttaacaaact
     3001 caaatttttg gtcgatttgt cgtagttttt cttgtatttc ctgttctctt ttatgtttcc
     3061 gctcaaatac gccatataat ttctgataat ctatgacttc ctgtccatat tctttcatga
     3121 gtttgagcgt atccatcttt cctaagtgcc gatgatccgc aaaacgatat acttcttgct
     3181 gaagaatctg tttttcttct ccagttctct tttgtttttc ttctgcttca gctaattgag
     3241 ctaaggctag atcaagttgt cctagttttt cttgccacat tccttttatc tcagagaaat
     3301 catttttagt tttaattttt ggttgccttt tgtagaagaa atagccaatc atgcctgcac
     3361 caattagtaa aaggatcact ttcaagggca ttggtacaaa tatacctgca atgattattg
     3421 ctccgccagc catcagcata tacggtttag atgaagagac tggtgctatc tctgactgat
     3481 tttgtaataa tgctggatag gtctcttcca agtcattcac ttctgcttca atcatctctt
     3541 gctgttcttt agctatttgg agacgcacag aaatctggct ttctagatca gcaatccttc
     3601 tttcttcagc cagaagatgc ttccattctt cttcatataa aaattcttcc ggtaacggat
     3661 ttttctgcca atgccacctt tgttccaaaa tcttcaattc tgaatttgtt tctctagtct
     3721 caatttgtaa tctttgccat tcttcgttta attgttccgc tgtaaattga agttgctgga
     3781 gatcttttag ttcctgctcc tgctctaaat aaaaataata tctggctgac tgctgatcca
     3841 tcccgctatg cttttccagt gttgcttcta attcttccaa acgctcattt gtttgctgat
     3901 accgtttgga gaaagactcc agatctaaaa gaagttgctc atcaggaaga cctgtagtgc
     3961 tgtttttttt gagagattgc cattcttcat ataaagaaaa attaagttct tgctgtctgg
     4021 ctaatgaaaa ttgtttgttc gcctctttta attgtgccaa taactgctgc tgttgcgact
     4081 cgtattcctg ttcttgttgg atcaacttgc caaaataagc ttcctgttct tgtttttgat
     4141 gaattttttc ttgcagctct ttccattcat ttaatttttg attgattagt aatttttggc
     4201 cttttttctt gaataattgt tgtgctttct gataatattc ttgccgcttt tggaatactt
     4261 gagaactgcc cgtgatgcct aaagaaatca gcgcatcatg tagctctttt tctctcaaat
     4321 gatctagttc catcaactgc tcttgctgga aagtaaatac ctgctggaat aactctttcg
     4381 tcaaaggtgc gatcagtttt tctagtaact catcacttcc cacttgatcc cccagccaca
     4441 ccttcgattt tccgcgattt tgctgcttgt agcgctcgac agcaaattca ccatatacag
     4501 gatgtttaag ccatattttg ccgccatagg ctgagccgtc ttttggtgta taatcctttt
     4561 ttttacggcc ttttgaaggg aaaccaaata atattgcttg aataaattga taaatcgttg
     4621 attttcctgc ttcgtttccc ccatagatca gctgattgcc tgaataaaag tcaaaacttt
     4681 tttgacgcca ttttccaaaa gcagcaatct cgatccttaa aatcttcatt cctgatcctc
     4741 ctcaaataaa aaatcttgat taagcagctg cttggctttt tcaagtgttt cttcttgata
     4801 ttccggcagt tcgctcaata ttctagccgc ttcttgatga ctatatactt catttaaaat
     4861 ttgttggaaa tcttgaggtg tctgatagta ttgaaataac tgttccatca atttagcaga
     4921 agcagcaagt ggggtcttcg tataaaaagt attctctatg agagaaatac gccaaataaa
     4981 gaaatcattt atcctatctg agaattcctc taacaaatag tctaatagct ctcctgattg
     5041 tatccgttcc aaaacatctg tattgagatg gtcataatct tttaatttta tctcaattaa
     5101 actaaatcca tcctctactt ttgaaagctg ctgacgaatg tgaacaatga cctcttttgt
     5161 cgttcttgct ttcctcaatg atatttcctt tgtcttccaa tagacctctg ctactttaat
     5221 cggatgtatt tggcaacttc ctttacttaa ttcaactagt agaatagatg tagaagcttg
     5281 ttccttttta gtatgacctt gaggcgcacc tggataaaca attgtttgct tttcatttaa
     5341 gaccgttggc acatggatat gccctaaagc ccaataatcg tatccctttt cttgcatctt
     5401 tgagggttgg aaaggagcat agtttccttt ttgagagacg ccaggttccc cgtggtacaa
     5461 cccaatatgg taatctgtga gttctctaga aggaaattcc atcactttgt ctttttgaat
     5521 ccacggttga cgataactaa atccactgag agagacagtt tctccacttt ttatcgttat
     5581 tttttttgtc tctaccgttt cggatgtaaa taaatggaca tttttaggaa aggagaacca
     5641 ataccgctct ttttgataga aatcatgatt tccgaatatt ataaatacag gtatatggtt
     5701 cttttctaac cgtttcatct gttctgaaaa atgtttctgt atttttaatg aaggacgatt
     5761 ttgatgaaaa ttatctcctg caagtaaaac aaaatcaacg ttatttatta ttgcctgttc
     5821 aatgatattt gataaaacgg tcagattcgt cttcaatagt ttttcctgta cagttttatc
     5881 aagagttgtc agtccttcaa atgaccgatc catatgtaaa tctgccgcat ggataaattt
     5941 caccattaaa aatttcctcc tttttttctt ttatcatacc attttcaatg ttttttttca
     6001 tcaataaccg aaaatttgtt cgtattatgc aaagaaatta cgataaatgg ccgacttcaa
     6061 aaaataagtt tattcgaaaa aaagattgtg acatgatttt tgtcacaatc ctaaatatct
     6121 atgttatatt cgcttcgttt gacactgaag aagtactatt tgtactggaa tttatgctcc
     6181 atttttccgt atttagaatt ctatgttcta tactacctta attagtttat atggtaagaa
     6241 ttctttcaaa tgcttgagat actccgtctt tatcatttga aacagtgaca taatcagctt
     6301 ttttcatgat ttcttgatga ctatttccca ttgcaaaact aatatctgca ctttcaaaca
     6361 tagataagtc atttaaacta tctccgatcg cgattctcaa aggtttcttt aaattgtaga
     6421 ttgattccaa acgatcaaac accacacctt tagatacatt ttgaggtacg atttcaatat
     6481 ttacaggtgc cgattcagta acagacacat ttagcatctt tttcaaatgg ttaaatatat
     6541 tag
//
LOCUS       DAZULY010000152         6540 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000152 DAZULY010000000
VERSION     DAZULY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.153"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1768
                     /gene="ade"
                     /locus_tag="ACG81A_002062"
     CDS             14..1768
                     /gene="ade"
                     /locus_tag="ACG81A_002062"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFS5015391.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(1866..2528)
                     /gene="trmB"
                     /locus_tag="ACG81A_002063"
     CDS             complement(1866..2528)
                     /gene="trmB"
                     /locus_tag="ACG81A_002063"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFS5015392.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(2598..3380)
                     /locus_tag="ACG81A_002064"
     CDS             complement(2598..3380)
                     /locus_tag="ACG81A_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFS5015393.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(3584..4795)
                     /locus_tag="ACG81A_002065"
     CDS             complement(3584..4795)
                     /locus_tag="ACG81A_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5015394.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(4788..5525)
                     /locus_tag="ACG81A_002066"
     CDS             complement(4788..5525)
                     /locus_tag="ACG81A_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5015395.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            5663..6097
                     /locus_tag="ACG81A_002067"
     CDS             5663..6097
                     /locus_tag="ACG81A_002067"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFS5015396.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            6090..6434
                     /locus_tag="ACG81A_002068"
     CDS             6090..6434
                     /locus_tag="ACG81A_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFS5015397.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
BASE COUNT         2097 a         1343 c         1137 g         1963 t
ORIGIN      
        1 gggaggcact ccattggaaa caacattaag gaaatatatc gaagtagcaa gtaaaaaaaa
       61 gcctgctgac ctagtgatca agcatgctaa gatcgtcaac gtctttacga aagaaatcat
      121 ggaaaaagat gtagcaatct gcgaaggaat gatcgtagga atcggtgaat acgaaggaaa
      181 aagcgtacat aatgctaatg ggcaatatct agtgccagga ttcatcgatg gccatgtgca
      241 tatcgaaagc tcacttttat caccaagcga attttcaaaa gtatccttgc ttcatggagt
      301 tactactgta gtgacagatc cccatgaaat aggaaacatt gctgggagta caggtcttga
      361 gtttatgata gaagatgcaa gacaaacact catgaatatc ttttttatgc taccttcttg
      421 tgtacctgta accccttttg agaccaatgg tgcaaattta aatgcaaaaa aattagcccc
      481 ttttttacaa aaacccgaag ttttaggctt agctgaagtc atgaactatc aagcagtagc
      541 aaataatgaa acagacatcc tagaaaagat ccaattgatg caccagcata agaaaaaaat
      601 tgacgggcat gcagctggta tcggcatgga agagttaaat gtttatcctg cagcaggtat
      661 tcggacggat catgaagcta caactgcaaa agaagctaaa gaacgtttgg atcttggtat
      721 gtatttaatg gttcgagaag ggaccgttgc aaaagatctg gcatcgctac taccggcgat
      781 cacacctgaa aattcaagaa gatgcttctt tgtcaccgat gataaactga tcaatgattt
      841 agtaaaagaa ggatcaatcg atcatatcgt ccgtcaagct attgcacttg gaatcgatcc
      901 attacaagcc attcagatgg caacattgaa tgcagcagaa tgttttgatt tgaaaacact
      961 aggtgcgatt gcccctgggt atcaagctga tttcttctta acagacgatc ttaccaagct
     1021 gccaattcat gaagttttct ataagggaac attagttgtt caatctgggc aactccaaga
     1081 ccatttattt gctgatcatt caaattcata tacgtgttgt ctgccaaaac taaacgctca
     1141 accattaaat ccacattcgt tagagctgct tctttcttct tcacaagccc atgtgatcga
     1201 aattatcccg aatagtctag tgactaaaga tttggtagaa aaagtggacc gacaaggcga
     1261 acgattcatt ccttcaatcg aaaaagacca attaaagatt gcggtgatcg agcgtcacca
     1321 tcaaacggga aacattggat taggcatagt gaaaggattt cagctgaaac aaggagcgat
     1381 agcgacaacg attgcccatg actcgcataa tctagttgtc gttgggacaa acgacgagga
     1441 tatgctgtat gctgctaatc ttttgattca aaaaggtggc gggatgaccg ttgtcaacaa
     1501 acagcaggaa atagcttgtg tttctcttcc tataggcgga atcatgacac aggaaccttt
     1561 ttcgatcgtc tatgaacagt tgaatgaatt aataactaaa gcacatcaac taggagcagc
     1621 taaaaccttt gatccttttt tgacactttc tttcttaaca ttatctgtca tacccgaatt
     1681 gaagatcacc gacaaaggat tattttcatt ttctcgcttc caattgattc caccttgtgg
     1741 aaaaaccgat gaaataaata cattttaaat caaataaatc ccagaaaata cattacatct
     1801 gtaatgttca ttttctgaga tttatttatg gatattaatg tttttatgaa agcagaaagt
     1861 tagtttcaca gacttttctt tttggtctta tcttgaaatt gagcttctac tcgataaata
     1921 cgttgacctt ttgaagaaaa tttttcttca tactccgtca taatattgcc ttcaaattga
     1981 ctttgatgta aatctaacca tacctgtttg aggatcatgc catattgaga aaaacttgcc
     2041 agagaatact caaacaatcc ttgattatct gttttgaaat ggatttctcc atttggacga
     2101 agaatctgtt catctacagc aagaaaagtc ttatatgtca atcgccgttt ttcatgacgt
     2161 ttttttggcc atggatcgga aaaattcaga tagatttgat ctacttcgct gtcagcgaaa
     2221 tactctgtca acgctgatcc atccacatgt agaagtttca ggttaggaag cggttgttcg
     2281 atcaacttgt ctagtgcaat cgaaacaaca ctgacctgca tttctactcc aatataattg
     2341 atttcaggat gggcttttgc cattccagtg atgaactggc ctttccccat accaatctca
     2401 atgtggattg gatgatcgtt ttcaaacaat tcattccatt ttcccttcca caatgttgga
     2461 tcactaatta caaaattcgg gtgagccgct aacatttccg ctgctcccgg acgatttcgg
     2521 acacgcatta tttatctcca ttcctttcct ctaattaaaa aggtctggcc aaatctccgc
     2581 agagtttggg ccaaacctca gccactgaaa attcgtttta attgtaaaat ttcttcgttc
     2641 atccgtcgat gatcttcacg tttgcaatat ctggtgattt cttggagcaa attcatggcc
     2701 gcataccaat gaaatttttc aagcatttcg tcagtcgggc gtatgccata actgatcagc
     2761 cactgattcc agctagacag tggcacataa tttcctaaaa tcgttgcaat atccaccgca
     2821 gggtctgcaa acatgattga atcccaatcg actagatata aataattttt acatactaac
     2881 caattacgat ggtttacatc tccgtgtact actgataagt tttccttatc tattgacggg
     2941 atctgctcac gaagaaaacg atggacaatt tgcagaaatt gatttttctg gagttcttcc
     3001 ggtaattctt tttcatattt ttgcagcatc ttctccggcg tcatgacacg accaccgata
     3061 cgggtcagca tggttttaag agaatttgaa tgatgtaaat ggtacaaaac atcaatcacg
     3121 tcgtttcttt gaccaatctc attaggagtc agcaagcgac cttccagcca ctcttgtgct
     3181 gtcaaagtat cgccattccc cgttcgtttt gtccaaacaa gttttggtgt aattccttcc
     3241 cttgaaagag cagccaacat cggagtcgtg tttcgtttaa taaagacttt gtcctcatca
     3301 cgcatgccta tgtatgtttt tcctgtgtcg cctttgatgg gacgtaaccg ccactcttgg
     3361 tctaactgaa actccatcat gcattctccc gtcctcttac tctttgtttt gcagtactta
     3421 ttattctagt cgcctgaaca ctacacgtca agcacatata cgttcttttt catttaattg
     3481 ccattcaaaa aaccagcttt tctcaaaaaa aatagaacga acagcattct tactgtcaac
     3541 gctctatttt tcattctcat ttttacccta cacagctttg ttttcagtct gacatcttct
     3601 tcaaacgagt tggtacatat agtttaacaa agagcatagt aacaatcaaa taagccgcta
     3661 gcggaaccca tcgctgctgc ccatctaata cgactgcagc aatcaaacca aaaataaccg
     3721 acgcgataat taatacccat gtgagtagat actgaatcgc ctgcttcttc tgattcttat
     3781 ggattggata aagctgaacg agaaccatat agcggaactg gttcgctaaa ggtaacagtt
     3841 ggaaaccgat caaatataaa aataagaaac cgataatcag actaaaataa aagtcgacta
     3901 ctccggcaat caataaccca cctataatta ctaatcggaa ataaagacca ctaaaatcag
     3961 aacctcttgc caatcttctt gcaaacaagt aaagataagt gttttttgat tctcttttaa
     4021 tatgagtcaa tataggatcc aaatagcgtc ttcttttgat ctttgccgta atttcaggaa
     4081 catctgtgaa taaattgata aattgataga tctgatgcaa cctatgttct tctacatgca
     4141 ccattttctc ccaatccaat cctttttctt gttgcttcca caaaaaccag taatatagac
     4201 ttgcctgtac taatgcacct gctattcctg cccatattag ccaataaaat gatagaaaca
     4261 aaaagatcag agaagaaaaa aaccagccta atttccaata acgaataact tgttgatcta
     4321 gtcgaaacaa ttctgctctc ttgatttgca gatgactgaa tttcaaactc cataatatga
     4381 tcagatagac gaaaaacatc gaaaaggatt gctccgtact aaccaccact aatggcatgg
     4441 ccaatccgca tgccaatatc aaaaagatca acggaaaaat gcaagaatag cgaaaagctc
     4501 gttccaaata ggtttgcatt tctttttctt taggaagtaa aaaaacaata tctgctgcct
     4561 ctgcaaaagt agccatctta cccacgaata aacagagcat ccaaaataca gagattatga
     4621 gccctccaat tgcaaacggt ggctgcaggg ttttcaacca attagaataa tataatccca
     4681 caccgccaat caagaaaaga caaactaaaa caaaatgatc gttcaataca taacgcatat
     4741 atttcatcat tttcttttga tgtcttgcta aacgtttttg aaaaaaacta ctcatgtgcc
     4801 tgctcctttg ttaaagcaat atagatttcg tccaaagaag aatccggcag ctgaaattct
     4861 tttctcaaca tctcaagaga accgtctgct cgtatctctc cgttatgtaa gaccacaaaa
     4921 cgatcacaat atttttctgc agtagctaaa atatgcgtag acatcaaaat accggctcct
     4981 tcttttttca tttctaccat caattcaagc aatgcattga tagctagtgg atccaagcct
     5041 aaaaatggtt cgtcgatgat atataaagaa ggctcgatca aaaaggcgca taagatcatc
     5101 actttctgtt tcattccctt tgaaaaatta gctgggaacc agtcaagacg attagataac
     5161 cgaaaagtct ccaacaactt ttctgctctt ttcattgcta tatccatagg tatatcgtag
     5221 gccatcgcag tgacttcgat atgttctctc aatgtcaatt cttcatataa agaaggagtc
     5281 tctggaatat acccaatttg tttgcgatat tcttccgtat ttttctttaa ggatagatga
     5341 ttgatttcta tcgttccttt tgaagcttcc agcagaccga taatgtgctt gatggtcgta
     5401 cttttccctg ctccgttcag tccaatcaac cctactatct ctccaggctg tacatcgaaa
     5461 ctgatatctt ttaacacagg gagatgtccg tacccccctg tgacatgatc gataattaaa
     5521 ctcattcttt ttcctccgcc atttattttt atttattata ccacgaagaa aatgacggta
     5581 ttttgttttt ttccttacaa tagctttaac ttatggtaaa gttaagctga agtatttatt
     5641 ctttatagaa aggatgtttt atatggaaaa ctgtattttt tgcaaaatca tcaatcaaga
     5701 aatcccaagc tataaaatat atgaagacga caaggtctat gcttttttag acatctctca
     5761 agcaacgaaa ggacacacat tagttgtgcc taaacgtcat gtagcagata tttttgaata
     5821 tgatcccgaa cttgcaggag aggttttttc gcgtgtgcct aagattgctc aagcattgga
     5881 aaaagctttt ccagaaatga aaggtcttaa tatcttgaat aacaaccgtg aactagccta
     5941 tcaatctgtt tttcattcac atatccattt agtccctcgc tacagcaaag aggatgattt
     6001 ttctattcac tttgtcaatc atcaagatag ttatggatcg gaagaactaa aagctattca
     6061 agaaacaatc gtaaagcagg tgtcctgcga tgattaaaaa ctttttcaaa ggactgttat
     6121 ttggagctgc tgctggcact gtcggaggtc tgttattggc gcccaggagc gggaatgaaa
     6181 caagaaagaa actgatcgaa gaattggaag aagcaacaac tctgacaaat gatttaaata
     6241 acagcctgaa tcattttaaa agcgcattga tcgaaacaaa agaaacagct gaatctgtga
     6301 ttcctgcttt ccaagaagca atcaaaaaag atatcgaaga ttttaaattc caagccgaac
     6361 cacgtatcac acaaatcaat gaacaagtag aaaaactaaa agctgatctg ccagagtatc
     6421 ctgaacaaat ataataatga ttattttctc aacagattta actatataag tgagaaaagt
     6481 ttgcgtattt cctaggaatg tgatgaaatt ttgaattttg tttgaaacta ccacgacaac
//
LOCUS       DAZULY010000153         6504 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000153 DAZULY010000000
VERSION     DAZULY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.154"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..745
                     /locus_tag="ACG81A_002069"
     CDS             8..745
                     /locus_tag="ACG81A_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS5015398.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            759..1574
                     /locus_tag="ACG81A_002070"
     CDS             759..1574
                     /locus_tag="ACG81A_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFS5015399.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1587..2234
                     /locus_tag="ACG81A_002071"
     CDS             1587..2234
                     /locus_tag="ACG81A_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5015400.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2250..2906
                     /locus_tag="ACG81A_002072"
     CDS             2250..2906
                     /locus_tag="ACG81A_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS5015401.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3068..3889
                     /gene="racE"
                     /locus_tag="ACG81A_002073"
     CDS             3068..3889
                     /gene="racE"
                     /locus_tag="ACG81A_002073"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFS5015402.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3892..5247
                     /gene="rph"
                     /locus_tag="ACG81A_002074"
     CDS             3892..5247
                     /gene="rph"
                     /locus_tag="ACG81A_002074"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFS5015403.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5248..5766
                     /locus_tag="ACG81A_002075"
     CDS             5248..5766
                     /locus_tag="ACG81A_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5015404.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5873..6373
                     /gene="cbpB"
                     /locus_tag="ACG81A_002076"
     CDS             5873..6373
                     /gene="cbpB"
                     /locus_tag="ACG81A_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFS5015405.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         2138 a         1095 c         1439 g         1832 t
ORIGIN      
        1 taattctatg gcaatgatta aatttgatca cgtggataaa tattatggca agttccacgc
       61 attaaaaaat atcaatctcg aattcgagaa aggagaagtt gtcgttgtca ttggtccttc
      121 tggttcagga aagagcacga tgcttcgctg tatcaatgga ttagagacga tctcttcagg
      181 taagctgctg attaatgata ctgacttgca tgataaaaag acaaaactga cagaagtccg
      241 aaaaaacatc ggcatggttt tccaacattt caatttgtac ccaaataaaa ctgttctgga
      301 aaacatcaaa ttagcaccaa tcaaagtgtt gaagcaagac caggcaactg cagtcaaaaa
      361 cgcggagaaa tttttgaaaa ctgtcaatat gttagataag aaagattctt atccttccat
      421 gttatccggg ggacaacagc aacgggtggc aatcgccaga ggtttagcga tgaatcctga
      481 gatgctgctg tttgatgaac ctacttctgc tttggatccg gaaatgattg gtgatgtatt
      541 agacgttatg aaaaaattgg ctcatgacgg tatgtcaatg attgtcgtga cacatgaaat
      601 gggctttgcc aaagaagtgg cggatcgtgt tatcttcatg gctgatggag aagtattgga
      661 agacagcaga aatgtgagag actttttcga aaatccaaat gaagtccgag cacagcaatt
      721 tatcagtaaa gttatcaatc attgatagga ggaaaaagat gcgcaggtta aaaaaaatac
      781 gcctgatttt ccttttaggc attgcttttc ttgcactagc cggatgtaaa ggaaatgggt
      841 tggcaaatga agatatttta gagcgaagca aatctaccaa tgaaatcatc tggggtgtca
      901 agtatgatac tcgattattt ggaatgatgg atattgagag cagaactgta caaggatttg
      961 atgtagatat tgcaaaagca atcactaaaa agatccttgg tgacaatgga aaaacagaat
     1021 ttgttgaagt cacttctaaa actcggattc cattattgaa aaacggaaat atcgatgcaa
     1081 ttattgcaac gatgacgatc acagatgaac gaaaaaaaca ggttgacttt tcagatgtct
     1141 atttcgatgc tggccaagct ttgttggtaa agaaaggaag ccaaatcaaa agtgtagatg
     1201 atttgaatgc ttctacaacg gttctagcag tcaaaggttc tacatcggct gcgaatatcc
     1261 gacagcatgc accagatgca aaaattttag aattagaaaa ctatgcagaa gcatttactg
     1321 ctttacaatc tggtcaggga gacgcgatga ctacggataa tgcgattttg ttagggattg
     1381 cagatgaaaa tccagagtat gagttagttg gtggaacgtt tactaatgag ccttacggga
     1441 ttgcgatcaa caaagggcaa gaaaacttct tgaaagctgt taaccaagcg ctagaggaaa
     1501 tgcatgcaga tggcacatac gataaaatct atcaaaaatg gtttccaaat gaaactgaag
     1561 gaaaagtaga gtaaggggga gaggcaatga gcgaattgat acaaacttat ggaccaatct
     1621 ttgtcgacgg gttcaagatc acattgtttt cgagtgtctt agcacttgtg tttagtttga
     1681 ttatcggaac gttaatggca atcttccaat tatcaaagaa taaagtagtg agcgccttag
     1741 caaaggcata tgtagaattt tttagaaaca ttccattatt aatcatcgtg atgttttttt
     1801 atgttgtcgt accgatttat tggtttaatc tagatggatt ccaagcaggt acgattggat
     1861 taacgatcta tacatctgct tttattgctg aaacagtacg ttcagggatt caaaccgtac
     1921 caaaaggtca aacagaagca ggactatctt ctggttttac ctattcagaa atcatgcgtt
     1981 atattgtttt accgcaagca ttcaaaatcg ttatcccgcc attaggcaac caatttatca
     2041 acctcgtaaa aaactcatct attttagcaa tcgtagctgg tttagatttg atgtatcaag
     2101 gagacttgat cgctagtgaa acattcaata ccttcgatac gtatatcatc gttgggttat
     2161 tttacttagt tatcacgcta ccgttatctt acttaatggt ttatcttgag aaaaaatggg
     2221 cggttcgttc atagaaggag gggaaataca tggattttag cggcgctttt tcatggatga
     2281 atatccgttt tttattggaa ggattaaagg ttactgttga agtttcattg ttttcgatca
     2341 tttttagttt cttgatcggt ggtctgacag gtgtgattcg ttttgcgaat atccctttct
     2401 tttccaaaat ccttggtgta gtgattgata ttatacggaa tcttccatta ttattgatca
     2461 ttttcttcac ttactttgcc ttacctcaaa tcggcattca gatgaacatc ttctggtccg
     2521 ctgtcgcagc attaacgatt tttgagtcag ctatgttgtc ggaaattttc cgagctggac
     2581 tcaatgctgt tccaaaagga cagatggaag caggattatc aacgggactg acttatgtag
     2641 aatcgatgcg aacgatcgtg ttaccgcaag catttaaatc aatgatcccg gcaattgtga
     2701 gccaattgat ttcactgatc aaagatactt cattagcagt aatcatttct ttacctgaac
     2761 tttctcatca ggcacgtatt atttatggac agaacaccaa ttatgtgctt ccgatgttca
     2821 tcatcatgac acttatgtac ttcgtggtat gttacgcgct ttctctgctt tccggttact
     2881 tggaaaagag acggtacagt tattaataga tgattctttt agcggttgtg gcaaactaaa
     2941 gtcacaaccg cttttcctct ttccattcga aaaaaagaga tttcgattgt tttaagcaag
     3001 taagagcact tgaaaattca cgctaaaaag tacacaatag ggaggataga atttattgaa
     3061 aaaaaggatg atacgattga cagataatcg ccctatcgga tttattgatt caggtgtcgg
     3121 cggcttgact gtagtaaaag aagccctgaa acaattaccg aatgaaaata ttttatttgt
     3181 aggagacaca gcacgctgcc catatggccc tagacccgcg gaacaggtaa tccagtatac
     3241 ttgggaaatg acggattatc tggtggagca aggaatcaag atgctggtga tcgcctgcaa
     3301 taccgcaact gcggtggctt tagaagaaat caaagctgct ctttctattc cagtcatcgg
     3361 tgtgatcctt cccggtacta gagcggcagt aaaaaaaaca caaaataaac aagttggcat
     3421 tatcggtacg attggtacgg taaaaagtca agcttatgaa aaagcactga aagagaaagt
     3481 accagaattg actgtgacaa gtcttgcttg tcccaaattt gtttcagttg tcgaaagtaa
     3541 tgaataccat tcatcggtgg cgaaaaaaat tgtggcagaa acattagctc ctttaaccac
     3601 taaaaaaatc gatacattga ttttgggatg cacccattat ccattattac gccccatcat
     3661 tcaaaatgta atgggagaaa atgttcaact gatcgattct ggagcagaaa cagtaggtga
     3721 agtatctatg ctgttagatt atttcaattt gagcaattca ccgcaaaatg gtcggacatt
     3781 atgccagttt tatacaactg gctctgccaa acttttcgag gaaatagctg aagactggct
     3841 tggaatcgga cacttaaatg tagaacatat cgaattggga ggaaagtaag gatgcgtcat
     3901 gacgggagaa aagcccaaga gcttcgcaaa ctggagcttc aaacaaatgt tttcaaatat
     3961 ccagaaggat cggtagtgat ccgttttggt gatacaacag ttatttgttc tgcaacaata
     4021 gaaaattctg ttcccccttt tttgagagaa acaggaacag gatgggtgac agcagaatat
     4081 agtatgctgc ctcgagcaac gagtacgaga aatcgtagag aaagcagcaa agggaaactt
     4141 tctggtagaa caatggaaat ccagcgtctt attggtcgtt ccttgcgtgc agtagtagac
     4201 ttggaaaaat taggagaacg tagtatcatc gtagactgcg atgtgatcca agcggatggc
     4261 ggtactagaa cagcaagtat cactggggca tttgtggcat tgaaattagc cattgaaaaa
     4321 cttttaagag aaaaagaatt gtcagaagat ccaataaaag aacatcttgc tgcagttagc
     4381 gtaggaatct taccagatgg gacttgcgtc acagatttgg attaccaaga agattccgca
     4441 gcacttgtag acatgaatct tgtaatgaca gaatctggaa aattcgtgga gattcaagga
     4501 actggagaag aagcaacgtt tgatggtgaa cagttgaatg aaatgctctt ttttggaaaa
     4561 aacgcgattg aagatttgat caaagagcaa aaacatgctt tgctaacaga gtttgctcaa
     4621 aatgatgagc ggatagaaga aacaaaaaca atcatcattg cgacaagaaa tccaggaaaa
     4681 gcagaagagt ttcgaaatat gttcaaagaa gcgggatatc atgtaaaaac attacttgac
     4741 tatcctgaat tgccagatgt ggaagaaact ggcagcacat ttgaagaaaa tgcccgatta
     4801 aaagcagaaa cgattgcaca attgcttgat cagccagtct tagcagatga ttcaggtttg
     4861 aaagtggatg ctttaggcgg tatgccagga atctattctg cacgttttgc aggggaacaa
     4921 aaaagtgatg cggggaataa cgccaaatta ttatatgaat tgacagatgt gccggatgaa
     4981 aaaagaacag cacaattcca ttgtacgtta gtattcgcgg cacccaaaaa agacagcctt
     5041 gtagtagaag cggaatggcc cggtcgagta gcccgtatac catctggcga aaatggcttt
     5101 gggtatgatc ctttatttat cccagaaggt aagaaacaaa cagcagcaga attatcgagc
     5161 gaagaaaaaa acaagatcag tcatcgggct caagcaatga aaaaattgag tgccgagtgg
     5221 aaacaatggc tggaaggaga gagatagatg cggtatttag tagtaagtga taatcatgga
     5281 gacagagata tcctcgttga cttagtagat cgttatcgtt ctgaagtgga tatctttttc
     5341 cattgtggtg attcggaatt agaagcttct gacccattat gggatacatt ccaagtggtt
     5401 caaggtaatt gtgattatgg accaggattc gaacagaaaa aagtgatcca aactggtcag
     5461 gatacagtat tcatgactca cggtcattta tcgaatgtcc gatttggttt gacacagtta
     5521 gcaatcgaag ctcgagctgc aactgccgat atggtgtttt ttggtcatac ccatcaaata
     5581 ggctgtgaga tggatcagaa aatcctgttt ttgaatcctg gtagcatttc tcagcctaga
     5641 ggcccgatcc aaatcccatc atatgctctt atcgaaagtt tagatgacca aatttctgtt
     5701 caatactata accgtgccca tcaggcagtt gaagagttgg ccttttcttt ttcgagagag
     5761 cgctgaataa aaaaactcta atttttaata aaaataagct ttattttatg aattttgcat
     5821 ccggtttcgg taaaatagta gtgttgaatg aaatagaaat ggaggccatc agatgattgg
     5881 acctatagta agagaaatgc tgctggaaaa tcaggaaacg tttttgattc ctgcagaaaa
     5941 tgtggcaaat gttatgtatc atcatccatt gtcccatggt ttacttgtgt tatcgaaggt
     6001 cggttattcc aaaattcctg tattaggaaa agatgatcac tttgtaggac tagtaagttt
     6061 atcagatgtg gtcaataaaa tgattgactt gcaaacaatc agcatggacc cgttagaagg
     6121 actaacggtt gctgatgtga cagaaacaga tgttccgaca attgatgaaa attgggaact
     6181 agaagaagta ctccatttgt tagtcgattc gcctttttta ccggttgtca cagaagatcg
     6241 gattttcaaa ggaatcatta caagaaaaga gctgcttaaa gccgtaaact tcatggtgca
     6301 tgaattagaa cgtcgtaata tattgtctcc gaaaatcgaa gaattaaaag aacaaatcga
     6361 tatcgtgagc taaagaacga gaaactaaaa gataatcaaa aaataagtag aaaaaacagc
     6421 gtaccagttt tgactttatc ttgaaaaaga ggctgggaca aaagcgttta actccaagaa
     6481 ataagaagga attcacgaaa attg
//
LOCUS       DAZULY010000154         6484 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000154 DAZULY010000000
VERSION     DAZULY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.155"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            98..1651
                     /locus_tag="ACG81A_002077"
     CDS             98..1651
                     /locus_tag="ACG81A_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFS5015406.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(2032..3816)
                     /locus_tag="ACG81A_002078"
     CDS             complement(2032..3816)
                     /locus_tag="ACG81A_002078"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5015407.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(3831..5552)
                     /locus_tag="ACG81A_002079"
     CDS             complement(3831..5552)
                     /locus_tag="ACG81A_002079"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5015408.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            5652..6413
                     /locus_tag="ACG81A_002080"
     CDS             5652..6413
                     /locus_tag="ACG81A_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5015409.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         2044 a         1185 c         1261 g         1994 t
ORIGIN      
        1 agagtacata ccaaattcat aaatttttta ttagtgggac tagtgattgt atggattttg
       61 acgcataatg aatgaagtat gagaaaagga gagacaaatg gatcaagaac tatttattat
      121 cgaagtatta tcagctaaac aaaaaattat cgagaaagcg aaaaaaagag cgaaaagaat
      181 aatgttcaag cttttaagct tagtattttt ctctggtttg ctttttttcg gagctgtatc
      241 gttcctatat cgccatatga gtgcagtagt ccgttcagct gaagggatgc aaaaattaaa
      301 taatttgtat cacttttcgt ggggagttgg cggaataaca attgttgtgc ttattgtttt
      361 tcttctactt atttggctcc gttaccatca attgtcgcgt catttcgatc aatggcttag
      421 tcaaagagca caacagtttt tacataataa accaacttct caagatgatg actatcttta
      481 ttttgagaag acttcgacga gtcaagcttt tcggatacaa aaaagtgatt gccagttatt
      541 gctgagatca cttgatgatt tacctatcta cttaggcgaa agcaaatcaa aattcggaat
      601 gaacacattc catatttttt tactagaaaa cgaacccaga tgggaaaaaa gccagtcttc
      661 gcctggtgga tacctgacaa ctaagatgag tacattagtc actggttttt tcctcattct
      721 tttagctgga acaatctatt atgggagata cactcagcca aaaatgtctt ctcatataaa
      781 tgctgcttct cacatgacaa tgaactcaac taacgatacg catatacaaa agacaaatga
      841 agggttgtta gctgatccta agcaaaaggt aaatttgaca acacaaaaga taaatgatct
      901 caaacttgat acagagacgc actctctcta tatgacaaca gatagtggtg ctaattgggt
      961 atttgtgccg attgattcgc aatggttgcg ttttggcgaa tacaccctga caacggggct
     1021 gattcctgaa ggttattgga tggacaacac atatgatatt tccgccgatt tctcatggtt
     1081 tatctattct tctggggatg atgtacgtct gctgacttca agagataatg gtaaaacatg
     1141 gcaaacgtct attatcaccc aatcagttca gccgatacgt tatcgaaaag cggtatttta
     1201 tggcaacgat ggtctattta tctgtacatc agccagtggg atgtctgcag aaactttgta
     1261 catttacact actatggatg gcggaatcac ttggataaaa agtggaagta caatgatcaa
     1321 tcagccagta caaaatgtca gcttcgttac aacttcttta ggttttgttt cgacacgtga
     1381 aagtttgttt tatacaaaca acggaggacg ctcattcaaa gaatcagttc tgtcgattcc
     1441 ggaaaactat cagacagggg ggatagatct ttttcaatca ccaaatgaag ttactcaaat
     1501 aagcgcgaat acgttagaga caaaatttta tttgttgaaa aaaggaccaa tcgatgcagg
     1561 gaaaatgttt gcctgcctct tccgttcaac tgataacggg gaaacatggc aattcgttca
     1621 gcaactatcg caagtagaac aaggagatta aagaagagag attgtgagcc tgacgttcag
     1681 caccgagtca caagaaacaa attcaaaaaa cagcttctca tatttgttga atagtgtaac
     1741 tcgtgattcg gagttgacaa gagcaacaag aagcgatttt gaagtatagc ttctcgtatt
     1801 ttacaagatc gtgtcttatt gccgataggt tgtctcttga acaccgttta ttcgtaaaaa
     1861 gggattgtga caagtctttt gtcactactt accgaacgaa actgaaggag ctatccaaaa
     1921 agtatcatta gtattattct ttgtgtttct agtggcacca ctagtacatt taaaaaagct
     1981 atcgatcatg gactaatcat acatgatcga tagcttttta gataatgttt gttacgctat
     2041 ctgaaattga ctattataaa gactggcata aagcgttggt gattggagta gttcagaatg
     2101 tgttcctttt tcgacgatat cgccattctt cattacaagg atcatgtctg cattttcaat
     2161 cgttgacaaa cggtgtgcaa tcacaaaact tgtcctatct tttgtcacag cttccattgc
     2221 ttgctgaatc aatgcttctg ttcgtgtatc tacgctagaa gttgcttcat ctaaaataac
     2281 gacaggagga ttcgctagaa tgactcgagc aattgtcaat aattgttgct gtccttgaga
     2341 taacgtgccg ttttcgctag aaatgatcgt atcatatccg tctggcagtg ttcgaataaa
     2401 gtgatcacat tgtgcgattt cagccgcttc gataatttgt tctcttgtgg catccatttt
     2461 cccgtaggcg atattatcag ctaccgttcc ttcaaacagc caggtatttt gtaaaaccat
     2521 cccaaaagaa cgacgtagtt ctccacgtga aagcttggta atatcaagac cgtcgaattc
     2581 gatctttcct ttattcagtt cataaaaacg catcagcaga ttgactaaag tcgtttttcc
     2641 tgcaccagtt ggaccaacga tcgctaccgt ttgttttggc tttactgaaa aatcgacttg
     2701 attcatcaat attttatctg atgtgtaacc aaactgtaca tttttgaaag ctatttttcc
     2761 tttcggttgt tctaaaagtc ttgcatctgg aaggtctgga atttcatctg tttcatctaa
     2821 tatctcaaat atcctatcga ttgctgccat tgcggattgg atcgagttga tcacataaga
     2881 agcggtagaa attggttcag aaatctgatt gatgtactga agataagctt gtaagaagcc
     2941 gatcgtcaca ccacctgata gaacaagcat agcaccgaat accgcactta tgatgaaggc
     3001 gagttgatta atgaaacgaa tggccggata gatagcacag ttcataaatt gagcacgttt
     3061 aaaagcgttg tattgctttt tatttacttc gtgtatagca gcttctgcat gagcttgctg
     3121 attgaatgtt ttgaccacta gattaccagc taagatttct tccattcgat tatttaaaac
     3181 acccagttca tactgattct gttcagaaat ctgtttgttt ttgttcgcaa tcttagttgt
     3241 aacaattgaa ctgccaacga tcagcaataa gacgagcaaa gtaagcttgc tttcgatata
     3301 aaacagcatc agaccagcag cagctattgt tacgacagaa gtaaaaaatt gattgatacc
     3361 tgtcaataac acttgagaaa gttgattgag accggtagtt gtccgactaa tgatgtcacc
     3421 tacttgatga ttgtcaaaaa acgacatcgg taatgttttg aatttttttg tgatttcttt
     3481 tcgcaccctc aatgtgatct tttcgcttaa cgctgccatt gtccgttcct gaataaatga
     3541 agtgatgctg cttaagactg aacagccgat cagcaataag acagggaaca acaatgcttg
     3601 tttgatatca ctgaaagaaa gctgtccaat acctgttttt cttatttctg ataagagatc
     3661 atcgatcccg attcccatga tcaagggcat ggctacgact aataggttcc cgaataaact
     3721 gcatagaaac aatccgtaaa aatatttatg ttcaggtttg atcattttcc aaaaacgtat
     3781 caaagcacgc gtgttcaacg tttttttctt ttccatcgta tcccttcttt ctatattcct
     3841 tgtgattttg caaactcctg ataagtttgg caattagaca gtagttcttc atgagtacct
     3901 tgtccaacga tttttccttc atctaaaaca atgatgttat ctgcttgttg gattgtactg
     3961 aggcgttgag caacaatcac gagagttttc tccgacatgt gtttatgaag ggctgctctt
     4021 aatgctgcat ctgttttata gtctaaagcg gagaaactat catcgaatac atagatctct
     4081 gctggtttga tcaacgctcg agcaatacat atccgctgtt tctggccccc tgaataatta
     4141 gaaccgcctt gagcaacaaa gctgtccaat ccgtcaggaa gggtgtccac tagttcttct
     4201 aattgagcga tcttgatcgc tttagacaac tcttctgttg aggcatattc atttcccatc
     4261 tgcaagttgc ttaaaattgt cccgctgaat aagaaagcct tctgagggac ataactgatg
     4321 tgggagcgga tctgttcttg agtcatttga gtaagaggaa taccaccaag ggttactttt
     4381 cctatcgtag ggtcactaag tctcaagaga agtttcgcca aactactttt tccagatccg
     4441 gtcccgccaa cgatcgctgt tgtttttcct ttaggtattg tgaaatcaat atcttctaga
     4501 actggatcag agatttcagt atatttaaat gtgacatgtt caaaagcagc tgcatttgtc
     4561 tgatcaaagg ttggttcagc tactgttttg atcgtctctt cctcaatttc gatctctgct
     4621 tgcagtattt cttctatccg tcgtaatgag gcaaacgatt tagggataat gaccaatacc
     4681 atagctgcga tcatcaagta agcaagtgtc agcatagagt attcgactac agcagtcacg
     4741 acaccgattt gtaatgctcc ttctgctaca agatttccgc caaaccacaa aatagaagca
     4801 taaacgattc ccatgagcaa aaaagcaatt ggagtgaccc aagcaaatat tcggttcact
     4861 ttgatcattt gattagcata gtcagcaaag ctcttattcg ttcgcttttc ttcatatgat
     4921 tgattatcaa aggcacggat catattaatc ccagtaaaga attctctcaa tgtgatggtg
     4981 atccgatcca tttttggctg gatactcaaa gataaaggtg cccctcgttt catcagtcga
     5041 taaacaatcc atacataaaa aacaacagag atcaatgtaa cccatgttag taatagagaa
     5101 taacgaaaca tcatgagaat ggtgaaaacg caaataaccg gtgctggtaa gatcatttgg
     5161 caaaataaaa caatcatttg ctggacatta tcgacatcat tcgtcattct tgtcaaaaga
     5221 gatccagaac taaaacgact gatatccttc attgacagct gttggaattt acggaaatat
     5281 ttctcccgcg tttggaatcc aaacctagcg gaagccaatg ctgaaaagta actagaggca
     5341 actgcagcga ttgttcctag aaacgctaca agcagcattt caactccaat tttttttata
     5401 actgaggctt cgccacttgc tatacctgtg tcgatcagct ttgctactaa ttttggtaca
     5461 cctaatgtac cggcgatttc taaacagatc atcacaaagg taaggagagc gagtcctttg
     5521 tttttctgta agaatgtttt taagatagac atattattcc tccttttttc ctgatacttt
     5581 tcgattctat accttcaagt aacttgaagg tcaatagaaa aaagaataaa ctgaaaaaga
     5641 ggtgagaaaa aatgaaaaaa atgtatacga ttggccaagt atcggaacta tttgatttgc
     5701 caaaatcaac gatccgctat tgggatgaac aaggattaat ccgttcaagt agacaagaag
     5761 agaatgatta tcgattgttt gatatcgatg atatttttat gctttatgat atcgtttttt
     5821 atagaaaatt agatataccg atcaaacaga tgaagaattt atatggaaaa acattgacgg
     5881 agttatatga gacacttgat gaaacagaaa gaagaatcca taaagaactg gtggtgatga
     5941 aacaaaaaca aaaagagata agggagcaaa aaaaacagtt gaaattgatg atcgatacaa
     6001 acgaggaaga atttcctgta gaagagatcc cttttgactg tatgatctca acggagtttg
     6061 aagatattgt ggaaatcaaa aaatttctac ctaactattc aagctttggg atgatgagta
     6121 tgccagcttc cagttcacag acgatgtatg gattttttat cgaccctagt gaagtacatt
     6181 tatttactca agacgtgatt tgggagaaaa aagatacggc tgtttatcgt cgattcttat
     6241 tgaaatccga gatgaaccat gcggaacgta ataatatttt agaaatcaga gaaagaatgt
     6301 atgaaaaagg atggaaaaca ggagaagtga tcggccagta tctattgacg aatacggatg
     6361 aaaacaatat cagaactgaa tattatcatg catggataga aatgaaaaaa taggaaatat
     6421 gtgtttaaga taactcgaaa atgatggttg ttttagttat aaagaaaaat tttctggttc
     6481 gatt
//
LOCUS       DAZULY010000155         6274 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000155 DAZULY010000000
VERSION     DAZULY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.156"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2465
                     /locus_tag="ACG81A_002081"
     CDS             <1..2465
                     /locus_tag="ACG81A_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFS5015410.1"
                     /translation="EVITQKKHSVRDNDIEKIRFVFIDIDPKRKEGSATDSEKKKAEN
                     VMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTIQNFLQLLHRRFGV
                     EDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPDNIDRSNCFKILEQ
                     IVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIKEGDHKGVTLLIFD
                     ECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKYPLPKPVNPSVESL
                     ESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQKVITLVLAYYVDHC
                     WDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENAPIFFEHESLGWKK
                     ENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKYVLGTNLELAWSIG
                     IASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGNPTTVLRNMNATNK
                     AIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLDSNCQLMPQETFSV
                     LTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKSLSSNTFGWGIKLF
                     LLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYSGALLIQDILGYPL
                     RLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLTNSDNKHTPSTIWG
                     KVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNMCAEKGRRTKRIHM
                     NRQVLPTYEILLPSSLKSYFRL"
     gene            2647..2886
                     /locus_tag="ACG81A_002082"
     CDS             2647..2886
                     /locus_tag="ACG81A_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015411.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            2902..4275
                     /locus_tag="ACG81A_002083"
     CDS             2902..4275
                     /locus_tag="ACG81A_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5015412.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4279..5913
                     /locus_tag="ACG81A_002084"
     CDS             4279..5913
                     /locus_tag="ACG81A_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5015413.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6076..>6274
                     /locus_tag="ACG81A_002085"
     CDS             6076..>6274
                     /locus_tag="ACG81A_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015414.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2192 a         1026 c         1277 g         1779 t
ORIGIN      
        1 aagaggtgat tacacaaaaa aagcattctg tgagagacaa tgatattgaa aaaattcggt
       61 ttgtatttat tgatattgac cctaaaagga aagagggaag tgcaacagat agcgaaaaga
      121 aaaaggcaga aaatgttatg gaacaagtag aacgttattt gaaagaaaag agaatagaat
      181 catttgtaaa agttgatagt gggaatggct accatatttt tcttcccata aatgaacaac
      241 caaataatca cgagacgatt cttactattc agaattttct tcaattactt caccgaagat
      301 ttggagtaga agatgaagtt gatattgata cttctgttta taatccttca agattatgca
      361 aattagttgg tacaccagcg gtcaaaggaa agtccacaaa agagcgtccg catcgtttat
      421 ctagtttcct ttctattccc gataacatag atagaagtaa ttgttttaaa attcttgaac
      481 agatagttga agaaaattcc atgatgtcaa cctctagagt aaaaagccat ccatctttta
      541 gagaagtgtt accttttatt caagcagact ataaacaatg gttagctcac tatcctgaat
      601 taacttatac cataaaagag ggcgatcata aaggagttac cttacttata tttgacgaat
      661 gtccattaag aaaacacacg aataattcta atggttcttg tttgtctgtt acggctgata
      721 ataagattcg atttcattgt ctgcatgctt cggataaaaa taaagatatt caagactttg
      781 taaaaaaata tccgttgcca aaacctgtaa atccttctgt agaatcatta gaatccaatc
      841 aagaatatta ttttgaccag tttaaactga atcaagaagg agtatttttg gtgaaagaag
      901 gtcaagaaga aaaagttgct tgtccgatgt ttattaagca aatacgcaaa aatatcgaac
      961 aaaaagtcat tacacttgtt cttgcctatt atgtcgatca ttgttgggat gaaatagaaa
     1021 tagaaggtgg atgtttacaa actaataatt ttaaacaatt agcaaaacat ggcttaatca
     1081 ttaaagctag aagagaagct gaagtcgttg actttttaca gattcaacgg gagaatgctc
     1141 caatcttttt tgaacatgaa tctcttggct ggaaaaaaga aaatgaagca atgatttttc
     1201 agttacaaga ttcttttgga cgtgaaattc cttctgtatt aactaaaaac tcatgctacc
     1261 aattaacaac taatggtaca gaagcggaat ttgacgcatt ggttcataag tatgttttag
     1321 gaacgaactt agaacttgct tggtcgattg gtattgcttc tattgtgtta ggctatttaa
     1381 acgttacgaa ggctaatact tttttgagtt tactaattaa cctacagggc aagagcacaa
     1441 cgggtaaaac cactacgtta cactttattg cctctttatt tggaaatcca actacggttc
     1501 ttagaaatat gaatgctacc aataaagcca ttataaagct agcatcaaac aatcagggag
     1561 gtgttccact aatactagat gagttaggag cagcaactca aaataacttt agctcgttga
     1621 tctatcaatt agcttcagga gaagaaaggc taagattaga ttctaattgt cagcttatgc
     1681 cacaagagac tttttctgtt ttgaccctta cctcttctga agagcgtttg gaaaattatt
     1741 tcaatgagaa attaactggt ttgaaagttc gatatatcga attttctgat atcaagttta
     1801 caaaggatgc gaatcacgca gagaaagtta aaagtctttc ttctaatact tttggttggg
     1861 gtataaagtt atttcttttg aaactctttg aacaagggat tacaaaaatt gaagcaactt
     1921 ttgtcgaagc tcaaaaaaaa ctatgggctg ttttacctga acattctttg aagcaacgca
     1981 ttggtaataa tctagctttg atttatagtg gagccctcct aattcaagat atactgggct
     2041 atcctttacg attagagcat atacagacgg tgcttattaa ttcgtacgaa cagttaattg
     2101 tagatactag tttaggaagt gagcaaacgg tatttgaccg agtgaaaaca ttgttggtga
     2161 tgaatagcca taaattttta acaaacagtg acaacaaaca tacgcccagt acgatttggg
     2221 gaaaagtatc aattcaaaaa aatggggata ttaaggtaaa tatttttcct atcccgtttg
     2281 aggacttgtt aaggcgagaa tttcaagtaa aggatctaac ctatcttttt aggcaattgc
     2341 ttgattcggg taatatgtgt gcagaaaaag ggagaagaac gaaacgtatt cacatgaatc
     2401 gtcaagttct tccaacatac gagattctat taccttcttc actcaaatcg tattttcgat
     2461 tgtaatttag aataaaccaa agtatgaaag tgaggcacca tgttatggaa atatttctta
     2521 ttgatttagc aaaacaactg attacttatc tgtatgatag cgagcaaacc caaacggaag
     2581 taaatgaagc ttgatctatg cttcatttac ttcattcaat caaacatgaa agaaaggaaa
     2641 attctaatga ctaaaaataa aatgacacta aaagcagagg ttcttctgta tattcaagaa
     2701 catttttcaa atcaagcctt ttttacaaaa ccaatttact tggatttcga aataagaggg
     2761 gtatctgctg gatctatcgg gggtactctt caagcgttga agaacgaagg atatttagaa
     2821 aatcactttg tgcaacgttc gttcaataga agagtcgtga aaaaatggta tttagttcat
     2881 tcatagagag gggaatcaag catgcgtgtg gcttgttatg atcgaacgtc tgtttatgaa
     2941 gaagcgaaaa ttagtaatga cgctcaatat gatgcagtga aagagtatgt tttagagaaa
     3001 tatgaacaaa ttccccaacg attttcagac aaaggagaat caggggaaaa agcggagcgt
     3061 ccagcttttc gacagcttag agcttgggtt tgtgcgtcag aagaagaggg ggtgatctat
     3121 gtttggcgtt atgatcgatt ggcacgtcaa gccaaaatag ccttagaatt tattgagttg
     3181 tgccaacaac atcaggttca agtgtattcg atttctgaac ctcttcctgc ttctttctca
     3241 cacgcgtcaa cgaataaact atttgtgcaa ttattgttca cttttgcaga acaacaacga
     3301 aacgtcatta ttgagaatgt tcgtgccgga ctttcctata aaaagcaaca acggcaatat
     3361 ttgtcttctg cggtaccata tgggtatcgt ttagtgaatg ggaaaatcca acaagaagta
     3421 cacgaagcaa ggattgttcg ctatattttt caactttatt tgaccaaaaa atatggctac
     3481 aaaaagcttt gtcaacgatt gacgcaacaa aagcttttct tccgcgaacg tccttttcag
     3541 ccctatcata tttatagtat cctaaaaaat ccattgtact atggcgaaat caaaggagga
     3601 tctttgggga agtatctagg aacctttgaa ccgattctct cgaaagcaac ctttttacaa
     3661 gcccaagaga ttcgccaatc aaggtgtaca gctaaaaaag atacgtatct gtacttatta
     3721 cgtcaaaaaa ttcggtgtcc cttctgtggt cgtcatctga gttcgaagta tcaatggaat
     3781 acaaagaaga cgaagacgct tcattattat cattgtaccg accgaagctg ccaaggaatt
     3841 tttcttcggg cagaaagggt tgagtcagaa gtattggaaa agatacaggc tttttttcaa
     3901 tacgaaggcg tctatcaagc cttgattgcc caattaacgc aacaattagc cgaaaagcga
     3961 aaagatgaaa aacgcaaagt aatccaacaa aagaaacaag aagcacaatg gattaaggca
     4021 tttgaagagg gcaattgttc actggaagaa ctcaagcaac aactgcaagc tcttcaaaag
     4081 ccgatgtcta cttcttcgtt tctttcactc caacagtgtg aagcaaaagt gaaagaatgg
     4141 cttcaactaa aagacctctc tagtcgacaa ttattatgtg actatgtttg tcggattgag
     4201 gtaacaaagg ataaaacagt ttgtgggatt tacttccgac aactgacaca aaatatttta
     4261 gaaaggaacg aatgatctat ggaaatgata caaaccaaac gagtcgcatt gtatattcgc
     4321 gtgagtacac aagaacaagc tttagaagga catagtattc aagcacaaga acaaaatgga
     4381 cgacagtttg ccgaacggat gggctatgaa gtggtagaag tttatgccga tgaaggaatc
     4441 agtgggaaat ccacaaaaaa tcggttcgcc tatcaacgaa tgatgaacga tgctcggaac
     4501 catcgttttg atctactagt gatttggaaa ttgacccgtc tcggacgcaa tatgttagat
     4561 gtcttacaaa cagtcgagga attattgcga tatgacgtag gattacattc gatctcggaa
     4621 caatttgacg tgaccacttc gacaggtaaa ttgatgttac aactccttgg gagtttcggt
     4681 gaatttgaac gcaaccaaat tgcggagaat gttcaaatgt caatgcaaag cttagtgaaa
     4741 gataaaaagc gttatgcagg aggccgacgt ttaggctatg tcagtggtat tgacacgaat
     4801 ggtcaaaaac aattggtcat tgaaccagaa gaggcgaaaa ttgttcgtct gatctatgca
     4861 aaatatttag gcggagcagg gtatcgagcg attgccaatt ttttgaatcg tcaaggatat
     4921 caaacagtca aaaagaatgc tttttctacc acggcagtca aagatatctt acgcaataaa
     4981 atttatgcag ggtatttaga atatgcgcgc tacatcaatt gggaaaccaa acgacggaag
     5041 gggaagaatc cgagaccgat tttagtgaaa ggaacccatg aaccgattat ttcggaagaa
     5101 gattatcaaa aagtacaaga acgccttgct ttagaaggaa aacaacccaa atggaaccat
     5161 acaggtgaaa atgtattgac aggcttgtta cgttgtccag aatgtggcgc accgatggca
     5221 gcgagtaatg tcacaaatac cctaaaggat ggcacaaaga aacggattcg atattattct
     5281 tgttcagtct ttcggaataa aggagcaagt gtgtgtcatg ccaattcgat tcgtgccgaa
     5341 caagcggaaa catttgtcgc cgaccgatta aaggagatcg ttcagctacc agaagttctt
     5401 ccccgattgg tggcagcgtt aaatgaagaa atagtgcgac aaagccaacc attggaacaa
     5461 gaactcgtgg tgttactgga acgaaaggaa gaattgaaga ctaaaattga aaaatgggaa
     5521 gctgctttgg aagatagtcc tgaattgttt ccgatgttaa aagaccgctt agacgaattg
     5581 actgaaaaac gacgacagtt gcacattcga gaaaatgaaa tcttagggat ctttcaacaa
     5641 ggagagccga ttcaagtcaa agatgtccaa cggatattga ctaatttgga tcgttttctt
     5701 gcccagagtg agaagaagca aatcaaagcc ttgtatcgta cctttataga gaaaataacc
     5761 tttgatccta accataagga gatgttggaa attacgatga aattcacgca tgccgtggta
     5821 caacaattga atgagcaata tcaaatagcc gtatccaaaa caaaggatac ggctattttt
     5881 gtgttaaaaa caccctttac gttaacgatc tagtctaggt tatatcaaac aacgttagag
     5941 gggcgctaac gcttctctag cgatttgaaa tcgttagaga agtagttagg gaggtcctta
     6001 tcataaatag ggatatttat taatgaatat ttgaaatata attgtatata agaattaaaa
     6061 agagtgggga tagatatgaa aaagatgggt aagtgtattt tatgtgaaaa atacactgaa
     6121 cttactaaag aacatgtacc acctaaaaaa tcagggaata ctggaggtaa aaagaccaga
     6181 actggtaatt tagatgattt ccttaagagc gattttacaa aaggtgattt tccgaagggg
     6241 attaaaagga aacctcaagg aaatgtttat tata
//
LOCUS       DAZULY010000156         6189 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000156 DAZULY010000000
VERSION     DAZULY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.157"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..242)
                     /gene="rpmB"
                     /locus_tag="ACG81A_002086"
     CDS             complement(54..242)
                     /gene="rpmB"
                     /locus_tag="ACG81A_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFS5015415.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            512..874
                     /locus_tag="ACG81A_002087"
     CDS             512..874
                     /locus_tag="ACG81A_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5015416.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            926..2608
                     /locus_tag="ACG81A_002088"
     CDS             926..2608
                     /locus_tag="ACG81A_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFS5015417.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2725..4761
                     /gene="recG"
                     /locus_tag="ACG81A_002089"
     CDS             2725..4761
                     /gene="recG"
                     /locus_tag="ACG81A_002089"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFS5015418.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4810..5811
                     /gene="plsX"
                     /locus_tag="ACG81A_002090"
     CDS             4810..5811
                     /gene="plsX"
                     /locus_tag="ACG81A_002090"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFS5015419.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5933..6175
                     /gene="acpP"
                     /locus_tag="ACG81A_002091"
     CDS             5933..6175
                     /gene="acpP"
                     /locus_tag="ACG81A_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS5015420.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2058 a         1077 c         1416 g         1638 t
ORIGIN      
        1 tttttattaa ataaaaaacc gttcactatc tgaacggtcc aagtatcatt caattaaacg
       61 cgttcaactt ttcctgattt caaagcacga gttgacaccc aaactttttt aggttttccg
      121 tcgaccataa cgcgaacttt ttgtaagtta ggtttaacag tacgttttgt tgagttcata
      181 gcgtgtgaac ggttgtttcc actttttgtc ttgcgacctg taaagtaaca aacttttgcc
      241 atggtaattt cctcctttgc cttgatttgg agcaatagaa taattcagct cataccagac
      301 taacatatca tatagaatgg gaatttgcaa gtacattttc atagatacaa ttgacaattg
      361 agaagaataa ttgtaaatta aacaaggtta tacctgaaat tttgcaaggt ttttgagtac
      421 tttgggtaac tgccttttat ttcaagttgc gctatgataa aatatgatag aagaaaaatg
      481 ggagtttttt acccgttaag gaggctcatt catggctgta aaaattcaaa cgtcagctgg
      541 aaccattgaa attaccaatg atgtaattgc aacagttgtt ggtggcgccg cgacagatgt
      601 gttcggtgtt gtaggaatgg ccagcaaaaa tcaaattaaa gataatatta ctgagatttt
      661 gcgtaaagaa aattactcaa gaggagtagt tgtccgccaa gaagataatg gtattgctgt
      721 agatgtttat acgattatca gctatggtac gaaaatctct gaagtttccc gcaacgtcca
      781 agaaaaagtg aaatacaatc tagaggcatt attaggcgta actgccaatt cagttaatgt
      841 ctttgtacaa ggtgttcgtg tactgccaga ctaggcagga gactggctgg taactcagca
      901 gtcgatctga aggaggatta tctaggtgag tgtaacagaa ataagtgcaa gccagttcca
      961 ggaaatggtc caggcaggtg cgaatcgtct gcaagtaaat gcagaatacg tcaattcatt
     1021 aaatgtattc cctgtgccag atggcgatac gggaacaaat atgaatctat ctatgactag
     1081 tggagctaag gcagtagtag attcaacctc agaaaaagta ggagaattag cagcaatgct
     1141 ttcaaaagga ttgctgatgg gtgcgcgcgg aaattccggc gttattttgt ctcaattgtt
     1201 tcgtggattt tctaagcaaa ttccagatgt tgtctcatta aatgcttctg atcttgctgc
     1261 agccttcact catggagttg aaactgcgta taaagcagta atgaaaccag tagaaggaac
     1321 gattttgaca gtagctagag aagcagcgaa agctggagaa aagaaagcaa aaagtactga
     1381 tgatgtcgtt gaagtaatga cagctgttgt aaaaggagca aagcgtgctt tagctaaaac
     1441 tccagacttg cttccagtcc taaaagaagt cggtgtcgta gacagtggag ggcaaggact
     1501 gctgtttgtt tatgagggct ttttaagtgc attgaatgga gaattccaag cggatgaaac
     1561 atatgaacct tctccagcag aaatggatga tatggtcaat gcagaacatc atcgcagtgt
     1621 ccaaggtcaa atggcgacag aagatatcca ttttggttac tgtacagaaa tcatggtaaa
     1681 aatcggagaa ggacctacag tcgatagtac attcgattac gaagaattcc gtaactattt
     1741 agatggtata ggagattctt tacttgtggt caatgatgac gagatcatca aagtccacgt
     1801 gcatacagaa aacccaggag aagtcttgaa ttatggacaa aaattcggtt cattagtcaa
     1861 aatcaaagtc gacaacatgc gcttgcagca tgagacgatt ttggaacacg atgaagaagt
     1921 gaacgagttt gcgaaaaaag aaataccacg tatcccgtat ggtatcgtag cgatcgctgc
     1981 tggtaaaggc gtccaagagc tgtttgaaag tttaggcgct aattatgtta tcagtggtgg
     2041 acaaacaatg aatccaagta cggaagacat tgttaaagcg attcaagaag taaacgcaga
     2101 taaagtgatt gtcttgccaa acaataagaa catcttcatg gcagctgatc aagcagcaga
     2161 agtagcagat atcccagtag cagttgttcc ttcaagaacg atttcccaag gattgacagc
     2221 tatgctttca ttcaatgatc aagcttcttt agaagaaaac aaagaaacga tgacagaaat
     2281 gttgtctagc gttgtgagcg gtcaagtcac tcacgcgatc agagatacct caattgacgg
     2341 tgtagagatc aaagaaggag actttttggg catgatcgat ggaaaaatcc tgatctctaa
     2401 cccagatatc acagcaacag cgttaagtac actagaacaa atgatcaatg aggacacaga
     2461 gatcgtaacg atcttggcag gtgatgaagg aacagttgaa caagcaaatg aatttgctga
     2521 gaaactaaca gaaaaacatc ctgatttgga aatcgagatc caccaaggag atcagcctgt
     2581 ctatccttat ctattttctg ctgaataatt tctgtgtcag aatatctttc taacacagta
     2641 ttcattcata aaaggccttc ggttagtgat caggtatgat cgctgaccaa gtcctttttt
     2701 tgttaaagga gggaggaaaa atttatgcgt tctttagatg atccaatcac tatgctggca
     2761 ggagtcggag aaaaaagagc agctagtctt gccagcttgg gcatccaaac gattgaagac
     2821 ttactgactt attatccttt tcgctacgaa gatattcagg aaagaaatct aaacgagatc
     2881 caggatcaag aaaaggttac tttgaaagga atcgtagtct cgcctcctgt gatgaatcga
     2941 tttggttata aaaaaagccg attgcaattt cgtatgatgc aagaacatga tgtgttcaac
     3001 gtttcttttt ttaatcaacc ttatctaaaa gaaaaagtca ttttatcgga ggaaatcgcc
     3061 gtatacggta aatgggatgc aaagcgtaaa gccttgaacg ggatgaagat tttaggttct
     3121 caaacaatgg atgacttttc gccgatatat cacgtcaaca aatcaattcg tcaaacgaca
     3181 ttagttgatc taatcaaaaa agggtttcaa gaatacggca cattgattga agaaaatctg
     3241 ccaaacaacc ttgtggagaa atatcgactg cttgaccgag cttctgctgt aagaagtatg
     3301 cattttccaa aagaccacga agaaaaccat caagccaaac gacgtgtcgt ttttgaagag
     3361 ttctttttat ttcaaatgcg tcttcaagga ttgaaaaaag cagaaaaagc agaaacgaat
     3421 ggtttggaga tcctttatga tgtccaaaaa ttgaaacaat ttactcaaaa gctacctttt
     3481 gaactgacaa atgcacaaaa acgtgtaacg aatgaaatct gtcgggatct tcgcagtccg
     3541 cagcatatgc agcgactttt gcagggagat gtaggtagcg gaaaaacagt ggttgccgcg
     3601 attgcgttat acgctaccgt tacagcaggt ttccaaggtg cgttgatggt gcctacagaa
     3661 atcttggcac aacaacatat ggaaagcttg caacagctgt ttgatcccaa tgaagtaacc
     3721 atcgcattga ttacggggtc aacgaaagca aaagaacgac gtgagctcct tgaaaaacta
     3781 gagcaagggg aaattaacgt cgtgatcggc acacatgcat tgatccaaga tggagtcgaa
     3841 tttcagaatt taggtctagt tattacggat gagcagcacc gttttggcgt gaaccagcgg
     3901 aaagtacttc gtgaaaaagg ctggcgacca gatgtcttat ttatgacagc gactccgatt
     3961 cctagaacat tagctattac agcatatggt gagatggatg tatctatcat tgatgaactt
     4021 ccagctggac gtatcccgat cgaaacacgc tgggtaagaa cacctcaatt ggacagtgta
     4081 ctggattgga cgaaaaaaga actggcaaga ggacatcaaa tgtatgtcat ctgtccgctg
     4141 attgaagaat cagaagcatt agatgtaaaa aatgcagtgg aaatctatga aaaacttcgt
     4201 gaactatttg caccaaaata tgaagttggc ttgttacatg gtaaaatgaa gaatcaggaa
     4261 aaagaagtaa tcatggaagc cttcaaagac aaccagatgc agatattagt ttcgactact
     4321 gtaattgaag taggggtaaa cgtgccgaac gcgaccgtca tgctgatcat ggatgcggat
     4381 cgatttggat tggcacagct gcaccaactt cgtggacgtg ttggacgcgg aagtgatgct
     4441 tcctactgta tcttagttgc taatcctaaa aatgaactag gtgtggaacg gatgaaaatc
     4501 atgacagaga ccaccaatgg ttttgtacta agcgaaaaag atctggaatt acgtggtcct
     4561 ggtgaagtat ttggcttccg tcaatctggt ctgcctcaat ttgcgattgc tgacttggtg
     4621 acggatggaa atgttttgga ggtagccaga gaagaggctg cagccctttg gcaaataaag
     4681 gattggcaga tgcttcctga gtatcagcct ttagcgcaaa gcctccaaat agaaaatcag
     4741 gataatcgtt atttcgatta gcagactgtg ctatacttta tactgatgac ggaaaaagga
     4801 gtggaaacaa tgaaaattgc cgtagatgca atgggcggag atcacgcacc tcaagcaatt
     4861 gtagaaggcg tggctttagc gcagcaagat tttccagaag tagaattctt actttacgga
     4921 aaagaagcag aaatcaagaa atacttaaca aatgaaaaaa acatcacgat tatccataca
     4981 gacgaaaaaa tcaatagtga tgatgagcca gtaaaagcaa ttcgacgaaa aaagactgca
     5041 tcaatggtcc ttgctgcaca agcagtgaaa aacggtgaag cagatgctgt attttcagca
     5101 gggaatacag gcgcgctact agcagcaggg ttgttcatcg taggacgtat caaaaatatc
     5161 gaacgccctg gcttgatgtc taccttgcca gtagtaggga aagaaggtgc cggtttcgat
     5221 atgctggatc ttggcgctaa cgcagaaaac aaaccagaac atttgttgca atacggtatt
     5281 ttaggttctt tttatgcacg taaagtccga ggtatcggac gtcctcgtgt cgcactctta
     5341 aacaacggaa cagaagcaac aaaaggtagt gaagtgacga aaaaagccta tgaactcctt
     5401 cagaatgaaa caagcttgaa ttttattgga aacgtagaag ctcgtgagct actcaatggt
     5461 gtggctgatg tagtggtcac agatggattt acaggcaacg ctgttttgaa atctattgaa
     5521 ggaacagcaa tgaatatgat gaatctatta aaatctgcta ttctaaatga agggatcaaa
     5581 ggaaaaatgg gcgctatgct tttaaaagac ggcttacgtt ctttgaaagc agaaatggat
     5641 tattccaaac atggcggtgc cgttttgttt ggcttgaaag caccagtcat taaaactcat
     5701 ggagcaacag gccccgatgc tgttcgctat acgatccgcc aaatccacac aatgcttgag
     5761 acagatgtag ttggacaatt agtcgaacaa tttgaaaaaa gcgaagaata gtatttggat
     5821 atctgaagcg atccgttcca ttgaaaaaaa ggtggacatt cctgaaaaat gccagtaaaa
     5881 ttagccatgg tgtaggtaat tgacaagtgt atgattcttg gaggtgaaaa agttgactcg
     5941 cgaagaagta tttaacaaag tagcgaaaat tatttcaaat cattttgaga tagataccga
     6001 taaagtgacg gatgaattga acatcaaaga tgatttaaaa gctgattcaa ttagtattat
     6061 ggaattcgtt ttagagcttg aagatgaatt cggtacggaa atttcagatg aagacgctga
     6121 acaaattgaa acagtcggtg gcgcagtaga ttatatcagc agccatctta aataatttca
     6181 aataaaagc
//
LOCUS       DAZULY010000157         6177 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000157 DAZULY010000000
VERSION     DAZULY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.158"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..915
                     /gene="ylqF"
                     /locus_tag="ACG81A_002092"
     CDS             55..915
                     /gene="ylqF"
                     /locus_tag="ACG81A_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFS5015421.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            908..1675
                     /locus_tag="ACG81A_002093"
     CDS             908..1675
                     /locus_tag="ACG81A_002093"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFS5015422.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            1731..2588
                     /gene="dprA"
                     /locus_tag="ACG81A_002094"
     CDS             1731..2588
                     /gene="dprA"
                     /locus_tag="ACG81A_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFS5015423.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            2710..4788
                     /gene="topA"
                     /locus_tag="ACG81A_002095"
     CDS             2710..4788
                     /gene="topA"
                     /locus_tag="ACG81A_002095"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFS5015424.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            4757..6133
                     /gene="trmFO"
                     /locus_tag="ACG81A_002096"
     CDS             4757..6133
                     /gene="trmFO"
                     /locus_tag="ACG81A_002096"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFS5015425.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         2139 a         1096 c         1390 g         1552 t
ORIGIN      
        1 aaaaaaacaa gtaaagccgg ggaatattcc ggtatgaaag gaatggagag aacaatgacc
       61 atccaatggt ttccgggaca tatggcaaaa gcccgacgag aagtttctga aaaaatgaaa
      121 tatgtggaca tcgtatttga attggtagac gcacgattac ctttatcttc tcgtaatcca
      181 atgctagatc agatcattca gcaaaaacct cgccttgttt tattgaataa agcggatctg
      241 gcagatccac aacagacaaa gctgtggcaa gaatattttc gtaaacaagg ccatcttgca
      301 ttagctatca cggcacaaga aagtaaagga attaaaggtc tagtacctgc ggctaaagaa
      361 gcattgaaag aaaaacgggc aagagatgct gctcggggga tcaagcctcg cgctattcgg
      421 gcaatggtct taggtattcc aaatgttggg aaatctacgt tgatgaatcg actagtaggc
      481 aagaaaattg cacagacagg aaataagcct ggcgtaacaa aaggtcagca gtggcttcgt
      541 ttaggtaatg agttggaatt gttagacaca ccagggattt tatggcccaa atttgaagat
      601 gaagaaatcg gcaaaaaact agcgctgaca ggcgcgatca aagaccagct tgtccattta
      661 gatgatttag cgatttacgg attagacttt tttgcccgat attattcagg aagaatcagc
      721 gatcgttatg gtttatcaat cgagcaagaa gaaaaattgg caccggaatt actgatggat
      781 attacagcaa aacgtgggta taaagaagac tatgatcgtg gcagcgaatt agtcgtgcat
      841 gatattcgtc aaggaaaact tggacgatac acactggatc gttgcgaaga aatggagcaa
      901 aaaagtgatg actgaatcca tccaaagtat caaagaacga ttgaaaagca tacagagaat
      961 agatgatcct acactagaat cattaagaaa tgatccacgc aaaggcgtgc agcaagcact
     1021 ggcttctttt gaacgacgat tgaaaaagga agcattaatt aaggaaaaat atgaagaaat
     1081 gttttgcttt gaaaagcaag ggtacgcaca aggtcaccaa tggattgccg gcattgatga
     1141 agtaggaaga ggaccattag ctggacctgt cgtggcagct gcagttatct tgccgaaaaa
     1201 tgagatcata ttagggctga atgactccaa acaattatca gagaaaaagc gagaacttct
     1261 ttatcaacaa gttcaagaaa aagccctggc aatcgggatc gggatcgttg atcagcagac
     1321 aatcgatgat gtgaatattt atgaagcaag taaattagcg atgatccaag cagtagaaaa
     1381 attgtccatt gtaccaagct atttgttgat cgatgcgatg accttggatt tgcctattca
     1441 gcaggaaaaa ataatcaaag gggatgcacg atctgtgtcg attgctgctg caagtatcat
     1501 tgctaaagta tatcgagacc aattgatgaa agaatatgac gtgctttatc ctggatatgg
     1561 tttctcgcat aatgcaggtt atggaacaaa ggaacactta gaaggacttg aacgacaagg
     1621 aattacgcca atccatcgta aaagttttgc acctgtaaaa aaatatttac aataaaaaac
     1681 agccgctttt acgtatctta ttagaaagag agacgaaagg cggttttatg atgtatcaaa
     1741 tagaagaaaa tttattgaaa cttgcttata caaaaggaat cagtagtcaa ggaaaatggg
     1801 cacttgctaa ctggatgatg cagtatcctt ataaagaaat cggatttgaa gacatcatta
     1861 agataggtaa aataaccaaa catcgggagc tgtttcgcaa aagttggcag gaaattcacg
     1921 ctaactgggg caaaatccaa tcgaaacagt cgtttatcac ttgctttgat cccaaatatc
     1981 cccctcagct gcttcacttg acctatccgc caattgtact gttttataat ggtgatctat
     2041 ctttactttc ttgtaacatg ttaagcgttg ttggtggaag acacacttct gctttagcca
     2101 aaaaaacagt ttattattta ctaaaaccag tgatagaagc tggatatgtg atcgtcagtg
     2161 gctgcgcgaa agggatagat acatacggtc accaagcagc aatctctcat ggtggacgga
     2221 caatcgcagt cattggaaca gggatagagc aagtctatcc tgctgaaaat cgtgacttgc
     2281 aagaggtgat tgcaaatgat catcttcttt tgagtgaata tcctcctcaa tccggcccga
     2341 aaaagcattg ttttccaatg agaaatcgga ttatcgcagc tttgtctaaa ggaacatgtg
     2401 tctttgaagc caagaaaaaa agcggctctc tgataacagc acagcaagca ttggatcttg
     2461 gaagaacagt tttttctgtt ccaggaaata ttttaacaga acattctacc ggatgtcatc
     2521 atttgatcca agacggtgca gtttgcacgg tttcgggtga agatatcttg gcagaattta
     2581 aagaataaca ccatactttt catttgcttt tcttgacaaa cgttttctga atggatatga
     2641 ttagctacga tttgtatgaa tcctctttta atagtaggaa cacgacaatg gaaaggagtc
     2701 cattcagtaa tggcgtataa atatttagtg attgtagaat cacctgcaaa agccaagaca
     2761 attgagaaat atcttggacg aaactataaa gtaatggcca gtgttggcca tataagagat
     2821 ttaccgaaaa gtaaaatggg tatcgatttt gaaaacaatt atgaacccca ttatatttct
     2881 atacgcggaa aaggcgatgt catcaaaagc ctgaaagccg cagcaaaaaa agctcaaaaa
     2941 gtttacttgg caagtgaccc ggatagagaa ggagaagcga ttgcttggca tttagcgtac
     3001 ctacttgggt tggatctgaa agaaaaaaat cgggtggtct tcaatgaaat cacaaaagac
     3061 gcagtcaaag cagcttttaa ggaaccaaga acgatcgatg tagatttagt agatgcacag
     3121 caagctcgtc gtaccttaga cagaatcgtt ggttattcga tcagtcctat tctctggcgt
     3181 aaggtcaaga aagggttaag tgcaggacgt gtccaatctg tcgctttaaa aattattatt
     3241 gaccgtgaaa aagagatccg agaatttgtt ccagaagaat attggagcat cgacggtaat
     3301 tttaaaaaag ctcgcaagaa attcaaagca aatttctggg gaatcgacgg taagaaaaag
     3361 aaattaccag atgcacaaag tgtaaaagaa gtcactgcta gaattgatgg aacagacttc
     3421 gaagttaaaa aagtagaaaa gaaagagcgc aaaagaaacc ctgcgaatcc tttcactaca
     3481 agtagcctgc aacaagaagc tgcccgtaag ttaaactttc gaacaagaaa aaccatgatg
     3541 gtagcacagc aattatatga aggaatcact ttgggtaaac aaggaacagt cggcttgatt
     3601 acatacatgc gtactgattc tacccggatt gccgatacag caaaagcaga ggccgcagca
     3661 tttattgaag atacgtatgg aaaagaattt tctagacatg atcaccgaaa gatcaaaaat
     3721 gctcaaggcg cacaagatgc ccatgaagct gtgcgtccaa cgagtgtgct tcgtacacca
     3781 gatgaactca aacaatattt agataaagat caattgaaac tttatacatt gatctggtca
     3841 cgttttgtgg ctagtcagat gacaccagct attttagata caatgaaagt gacattagaa
     3901 caaaatggtg tcatcttcat tgcgaacggt tcaaaaatca aattcaaagg attcatgcaa
     3961 gtgtatgtgg aaggtagaga tgacggcaaa gaagagaaag aaaatattct tccagaactt
     4021 gaagaaggcg atgtagtcca atctgttgat attgaaccaa aacaacattt tactcaacca
     4081 ccagcacgtt ttagtgaggc tacgttgatt cgtgcactag aagaaaacgg ggtagggcga
     4141 ccttccactt atgcaccaac acttgatacg atccaaagac gctactatgt aaaattgaca
     4201 caaaaaagat ttgagccgac tgaactagga gaaattgtca attcattaat ctgtgaattc
     4261 ttccctcaaa tcgtcgatat ccatttcaca gcagaaatgg aaggagattt ggataaaatt
     4321 gaagaaggca cggaagcgtg ggtcaaagtg gttgatcgtt tctataaacc gtttgaaaaa
     4381 gaactgacga atgcggaaga aaaaatcgaa aaaattcaaa tcaaagatga accagcagga
     4441 ttcgattgtg atgtctgtgg acatccgatg gtgataaaat tagggaaata tgggaagttt
     4501 tatgcttgca gtaattttcc tgattgtcgg aatacgaaac caatcgtaaa agaaatcggt
     4561 gtgacttgcc cagtatgtca tgaaggacaa gtcattgaac gaaaatctaa gaaaaacaga
     4621 ctattctatg gttgttcccg tttcccagaa tgtgaattca cttcttggga taaaccagtg
     4681 ggcagaaatt gtccaaaatg tgacggatat ctggtcgaga agaaagtcaa aggcggtaaa
     4741 caagtagtct gtgtaaatgg agattacgaa gaagacatcc aaaaataata gaaaagaggt
     4801 tctttcattg aaaatggtaa atgttgttgg agcagggctt gccggaagtg aagctgcttg
     4861 gcagatcgca caagcaggag tccctgtccg tttatatgaa atgcgtccag tcaaatcgac
     4921 tgaggcgcat cacacaagta attttgctga actagtatgt tccaattcat taagaggaaa
     4981 cagtttggct aatgcagtag ggttattgaa agaagaaatg cgccgattga attcggtagt
     5041 aatcacttcg gcagatgaaa cagcagtgcc agctggtggt gccttagctg ttgaccgcga
     5101 ttctttttct gaaacaatta cccgaaaagt caaagagcat ccattagtaa cagtgataaa
     5161 cgaagaaatt actgaaatac cggaaggaat caccgtagta gcaacaggac ctttgacttc
     5221 tcatccatta gcagaaagca tcaaagcatt caatggttca gatggttttt atttctatga
     5281 tgcagctgca ccaatcgtag acaaagctac gatcgatatg gataaagtat atttgaagtc
     5341 ccgctatgac aaaggagaag cagcttactt gaactgtccg atgacagaag aggaattcaa
     5401 agctttccat gaggcactaa tcaatgccga agtggcagaa cttaaatctt ttgagaaaga
     5461 aaaatatttt gaaggatgta tgccaattga agtaatggca cagcgaggat tcaaaacgat
     5521 gcttttcgga cctatgaaac cagttggatt ggaagatcct aaaactggca aacgtcctta
     5581 tgctgttatc cagctgcgtc aagacaatgc tgcagcttct ttatacaaca tcgtgggctt
     5641 tcagactcac ttgaaatggg gcgagcaaaa acgtgttttc cggatgattc ctgggttaga
     5701 aaatgctgaa ttcgttcgct atggtgtgat gcaccgaaac agctttatga attcgccaga
     5761 attgctgaaa ccgacttacc aatcaacgaa acgcgaagat ctgttctttg caggtcagat
     5821 gactggtgta gaaggctatg ttgaaagtgc agcgagcgga ctacttgctg gaatcaacgc
     5881 cgcacgctta gcaaaagaaa tggaaccagt cgagtttcca caagaaacag ctattggaag
     5941 tatggcttac tacattactc atgcggaagg aaaacatttc caaccgatga atgccaacta
     6001 tggtctgttt cctgaactgc cagagcgcat tcgagacaaa aaagcaagat atgaagcaat
     6061 tgcacaacga gctttaaaag ctaatgaggc aataaaaaca gaattagaaa aaactgcaat
     6121 cccaaatgag taaggaaact ccatgaagag ggtgagacgg atagtctcgc tcttttt
//
LOCUS       DAZULY010000158         6169 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000158 DAZULY010000000
VERSION     DAZULY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.159"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..1619)
                     /locus_tag="ACG81A_002097"
     CDS             complement(111..1619)
                     /locus_tag="ACG81A_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFS5015426.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1778..1969
                     /locus_tag="ACG81A_002098"
     CDS             1778..1969
                     /locus_tag="ACG81A_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015427.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2172..3089
                     /locus_tag="ACG81A_002099"
     CDS             2172..3089
                     /locus_tag="ACG81A_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFS5015428.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            3082..4155
                     /locus_tag="ACG81A_002100"
     CDS             3082..4155
                     /locus_tag="ACG81A_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015429.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4303..4821
                     /locus_tag="ACG81A_002101"
     CDS             4303..4821
                     /locus_tag="ACG81A_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015430.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4802..5194
                     /locus_tag="ACG81A_002102"
     CDS             4802..5194
                     /locus_tag="ACG81A_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFS5015431.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5270..>6169
                     /locus_tag="ACG81A_002103"
     CDS             5270..>6169
                     /locus_tag="ACG81A_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFS5015432.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIE"
BASE COUNT         2032 a         1086 c         1240 g         1811 t
ORIGIN      
        1 gaacaaaata aaaggtttaa aaagtgtaac gtcttagtaa agtctataaa aaaacgaaac
       61 tatcgattaa gctaatagtt tcgttttcaa catttataag taaacttttt ctattggtaa
      121 tatagatttt cggaagggta gacaggtgcg caggtaatat cgattcctag ttttttcaat
      181 gtttgttcat catttttatt gaccatcaca gtagagtggg cttgtgctcc ctcaagttca
      241 gctagtttat cataagcaac ttgagcgatt ggattcgtta ctgcactgat tgataaagcg
      301 atcaagactt cattgatgct tagtgcatcc aaagaggaat gaagtgtctt tgatttcaat
      361 ccttgtattg tttcaagaac taatggtgac aataaaggaa tgtcatctgc gatatgggct
      421 aaatgtttta ctgcatttaa gataactgcc gctgacgcat ccatcaatga acttgtacgc
      481 ccggtaatga tgacttcatc tggtaattcg attgcaatca cagcagaagg atcttttgta
      541 ggactttgcg cttgaatctt atctgcatag tcatgggcag ggactaccac gtttcggtct
      601 tcaggacgta aaccgacttc ttccataatc agtttgattc gattaactgc ttcttcgtcg
      661 atcaatcctt ttttgaagtc gcattctgtt tggaaataac gacggatgat ttcttgttta
      721 gaggcttctt ggacaacggt atcatccacg atcccaaatc ctacacgatt cacacccatg
      781 tcagtaggag attgataaac agattcttta ccagtaattt tttcaataat acgtttgatt
      841 acaggaaatg tctcaacatc ccgattatag ttcacagcta ctttattgta ggcatcaaag
      901 tggaaggaat cgatcatatt gacatctttt agatcgactg tggctgcttc ataagcaatg
      961 ttcagcggat gtttcaacgg aacattccat actgggaaag tttcgaactt ggagtaacca
     1021 gctgcatgtc ctttttggct ttcatgatac agttggttca agcaagtagc gagctttcca
     1081 cttcctgggc caggagctgt cactacaacg attggtttag ttgtttcaat atactcattt
     1141 tttccaaaac catcttctga gacgattttt tctacgttga ctggatagtc ttcgatttct
     1201 gagtgtttat agaccttgat gtttcgtctt tctagtttat tgataaagac tttcgtggca
     1261 ggctgtccac tatagcgagt gataacgaca ctgttgatta ataagccata accacgtaat
     1321 tcatcgatta accgcaagat atccatatca taagtaatcc cataatcacc acggatctta
     1381 ttacgttcaa tatctccagc atagacgcaa atcagtattt cagcttgatc tttcaatttt
     1441 tgcagcaatt tgattttcgc atcttcttca aatcctggaa gtacacgttt cgcatgcttg
     1501 tcgtcaacta acttcccacc aaattctaaa tataatttgt catagtggtt cactcgttcc
     1561 aaaatatacg ctgactgctc ttcaatatat ttttctgaat caaaaccaat ttttttcact
     1621 aggttccctc cttaagtact aaagcatttt ttcatttttt tgaggattcg tcaactggaa
     1681 gagcgaaatt cctcaaaaaa acaaagaaaa tggtttaaac caacttgtct agatagttat
     1741 tcattggtat gatagttttt gataaggaag gtgctacatg aaaaaaagac agaaaaagaa
     1801 aaacgcatat aaacactata tccgttcgat ttttaccggt tatgaaagaa tgcttgaaga
     1861 tcccgaattg gaacaattga cgtttactta tctaaacgaa gaaacgcagc taactcgtga
     1921 tgagcatcag cgcattcatt tcacaacaag agatctgcct agtaaataaa aaataagata
     1981 aaggtcatcg gattcgattc tgtcctatca gatggaaaga ctagttgatc gaaaaaaagg
     2041 ctgtaccaaa ttttggtgca gcctgtttta tggagcattg cccgctcttt tttataacaa
     2101 ttttttacag aaaatggtac tattgaagta ttaataagtg aaaaacagat gagtaagagg
     2161 aggcaagtag tatgaaagag ccagatacaa atgcaaatta tgagccagac gaactagagg
     2221 agacgaacgc ggatcaggat acaggacaag aatcgtcttc tttttacgat atcaaagaaa
     2281 aagcaagcat gaatctttcc caattattag aagacttaca ggcttttgaa aaagctgtcc
     2341 gcgaagaaga tatccctgag atctaccgtt tatacaatgg ccgattgaat gaagaattaa
     2401 aaagaagctc aaatgaaaat cacgagatcg atcaattgct gatgcgtaag atccatgatc
     2461 gttttttaca ggaatttcct tttatggaac aagtagaaaa catctcgcca acgatgagct
     2521 attacaaaat cggcacttat taccatgatc ggcccacggt cggtattgat gcaagtctgc
     2581 cagaaatttt tgttttgcct aagatcgatg aagagtggga gaaatattcc caaccggagt
     2641 caacggacta tgacaaaaaa atcaatgagc tagacgcaaa ggtgatcact gctcagacag
     2701 aaattgaacg tttagatgaa caaataaaag aaatcaatcg acaaatgact gatttgaacg
     2761 acaataaagg attcttgaat cgtaaaaaaa tcgaggaaga aattcaagaa ttagagaaga
     2821 agaagcaagt actatctaat gaaaaattag gctggcttcc ttatattgag acgcctgaaa
     2881 caatccagca gcaaaaagaa gcattgaagc aagaagcaag agcagaccag cttcgtgcag
     2941 ccatcgtaga aaaagaacaa aggcaaatca gacgctattt cggtagcaaa gagggattcg
     3001 gtcaagcaat ccatgaattc ttgatgaatt atcttggcaa tgagaatcct aataatcaat
     3061 ctaatgaagg aggttccgag tatgaatgaa cacgatgaag cagaatggac aaaaaatgaa
     3121 gaacaaacag aagatcatga agaaacgaac acttcattgg aagaacaaac aagtaatcct
     3181 ctggatgaca cagaagaaat ccctatgcta gaggaaacaa aaatacatga aaaagaaaaa
     3241 gtaactccga cgaatacgaa tactgcagaa aacacccatg cttacgaaat aactgaaaat
     3301 cccgggtatt tagaaggaaa agagcaagat catcaagata tcgtcagttt gattgaaaca
     3361 gcggaagcag aattagcgga tttacgtatg agaaaagatt tgatcgaatc agattttcca
     3421 caattattag aatcatatga tcgttttata ttgaatgaag aaccaatctc cacgatcgga
     3481 aagacaagcc tgatcgaata cttcacgtat gaattactga aaccgttgaa tgatattggt
     3541 ctggaacggt caaattcagc ttatgacaca tggaaaacaa gacatttttc gatcaattat
     3601 acgttgaatg gacaaaacga attagtcttt tcctttgtat taccgaccat cgatcaacga
     3661 taccaagtgg aatcgatgag tctcctaaac gtatcaccag agacaatgga aatcaatgtc
     3721 caagatgacc gggtgctttc tttgatccga cactggtcag tggatcgagt attctctgca
     3781 gggcagatca cgatattcaa tcacaaattg aatcaattat tagcccatgc aagaacactt
     3841 ggttttaccg ttaatcagac attactagac aatacgaagc cacttcgttt gaatcttcag
     3901 agcgaatttg aattgacgaa tgaagtattg gatgatattt ttatcgtggc aatgaaaaat
     3961 ccatcctacg atatggaaaa gatcaacgaa actacttatc aagtacgatt agataaagga
     4021 cagtctttaa cgataacaac aaacgaaaaa gatcaaacag tcttgtcgat ctcatctgga
     4081 gactacccac gatctgtcat tgatttcttt atcaattacg aattcttggt cccattaatg
     4141 gtgagaaagt cctgattgga taagttggat taccagatgt cattcagata attctgttcc
     4201 aaaggacttg cggcagttat ccagaataat tgtcacaagt cttttctgta tgtactaaat
     4261 ggacgtgaat aacttgctga tgaaggaggg atttcattag gaatgtccac aaataaaata
     4321 gcacagctta gcttattgtc tgctgcttgt gtagcaggta ggatcgcttt tcaatttatt
     4381 ccgaatttcc agccaatgac tgctattttt ttatttatag tactttactt aagcttaaaa
     4441 gatgcgttaa ttgtcatgag tctttctatt gcgatcagca gtttttattt tggtgttgga
     4501 ccatgggtca tcggccaatg gatcagctat actgttgttc tgagtttgtt ttcaatcgtc
     4561 tgcttgagaa aaacagtgca aaggaatcta tggttaaagg gagtctgttt ttttctggcg
     4621 ggcatggttt acggaatagg tatgaccgtt tttgatacgc tgttatatcg tttgcctcaa
     4681 ccttggatct attaccttca aggggtgtcg tttgatttga tgcacagtat aggcaatgtt
     4741 gtgttctttc ttgtttttct gccagtcgtc aaacgttttt acaatcattc aggaggaaaa
     4801 aatgaaaaaa ataatttata gtttgcttgc aatgagtgtc agtatactca ttttgacaag
     4861 ttgcagtaca acctatacac aatcggcttc caaccaaaag gaggaagtaa aaacatcgca
     4921 aaaagcaaca atcacgttac aagaaaatgg tcaaaacttt tcggaaaaag aagtggtctt
     4981 caaaaaaggc gatgatttac tgagtatttt gaaacgaaat tttgaagtca aagaagataa
     5041 cggcttcatc acttcgcttg aagggcactc acaggatgag aaaaatcaac tctattggat
     5101 gtttactgtg gacgggaaaa gtgctactac tggtgccaaa gagatcaaat tgaatgatgg
     5161 acaatcggtc attttcaatt tgtctaaact ataagtttca taaaaaactg ttaaagggcg
     5221 aatattgcca taattcagtg agttcagttg atgaaagaag gaaaataaca tggaaaattt
     5281 taaaaaaagc ggaatgtttt ccgtaattgc tgctctaggg gctaatattc tagttgctat
     5341 cagcaaattt gtcggctatg caatcagtgg cagtgccgcc atgctaaacg aaagcatcca
     5401 cagtatcgtt gattgcagca atcagatttt gctgttattc ggtgataaac aagccaataa
     5461 aggacagagc gagttgcatc aatttggtga aggacgagca aaatactttt tcagtacaat
     5521 cgtcgcaatg atgcttttct tcggcggtgg ggcacttggg gtgatggaag cttttgaaaa
     5581 gttgacgcat ccatcccatg aagtgggtaa tccgatcatt gttatagcaa tactgttatt
     5641 tggtttggta atcgaaggaa gttcgttacg cgtggcaatg aaagaaatca aagaattgaa
     5701 tactgaaaaa ctttccacga tgcgcttttt gcgcgaaagt cgacacagtg aaatactgat
     5761 tatttttacg gaagacttgt gtgctgtaat cggtttggtc ttggctttag caggaacaat
     5821 attgacttat gcaaccggga ttgctgcatt tgatgcgatc agcggattgc tgatcggttt
     5881 cttgctgatg ggagccgcca ttttcctagc aaaagaattt tatagtctga tcatcggtga
     5941 aagcgtgaca gcaagcgatt tatcaaaaat caagttagct tttgaacgtc ctgagatcag
     6001 tcggctgatc gatataaaaa cagttcattt aagtccaacg gagattttag ttgcagcaaa
     6061 agcagatatc gttggtaaca aagagaatga agcttactcg gtcatcaatg atattgaaaa
     6121 aaatatgcgt cagtctcttc ctgacaagaa gatgtatata tacattgaa
//
LOCUS       DAZULY010000159         6057 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000159 DAZULY010000000
VERSION     DAZULY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.160"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(268..753)
                     /locus_tag="ACG81A_002104"
     CDS             complement(268..753)
                     /locus_tag="ACG81A_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015433.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(892..1107)
                     /locus_tag="ACG81A_002105"
     CDS             complement(892..1107)
                     /locus_tag="ACG81A_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFS5015434.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1331..2131
                     /locus_tag="ACG81A_002106"
     CDS             1331..2131
                     /locus_tag="ACG81A_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS5015435.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2150..4018
                     /locus_tag="ACG81A_002107"
     CDS             2150..4018
                     /locus_tag="ACG81A_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFS5015436.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4011..4502
                     /locus_tag="ACG81A_002108"
     CDS             4011..4502
                     /locus_tag="ACG81A_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFS5015437.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            4490..5044
                     /locus_tag="ACG81A_002109"
     CDS             4490..5044
                     /locus_tag="ACG81A_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFS5015438.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5062..6057
                     /locus_tag="ACG81A_002110"
     CDS             5062..6057
                     /locus_tag="ACG81A_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFS5015439.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2132 a          966 c         1214 g         1745 t
ORIGIN      
        1 agtaaattct tctatccaaa tatagaaaaa ttgaatctag aaaaccgccc gacaataaat
       61 ttcattaact cagttgaccg aaaatgaagt agataccaaa aatttatcaa gagagcaagt
      121 agagaagtga gtatattcat aagagaaata gctgtaacga attattaggt taatgcaaat
      181 gcttatttat actccgtata actttctcag aaaaaatgat accaataatg acttgctagt
      241 actgtaaatc cgtataatat catctaatca aaatggtgtt ccatttgcat ctgtcatcgt
      301 aatcgatcct tcgggtgaca ctcgataaaa accaacgctt ccagatcctc cttgggaacg
      361 gatttgtttg ctggaagctg tgaacaaata atctcgatca attttttgat aaaagctaat
      421 aacaatatcg tcactttgta attcggaacg gtgggcaata agaaatagaa gtgctttgtc
      481 agagttttct aatggaagag aaggtacagt gctttcttgc atttcagatg ttgaagaaat
      541 gctaaacgac tgaatacttg attggctatg ttgcgaatct acattaccag tagaagtatt
      601 agtaatggaa gtagtagaag tacttttagt tgaaccaacc tcagttgttg aaatggctgc
      661 tgaagattct ttaacagaat tctttgtttt atatccacag gcagaaaaaa gtattacagt
      721 tcccaacaag cctgcgtaaa tccctttatg catgatagct cctcctaaaa tagattatct
      781 ttattcagta tatcacttaa acaaagaaaa gcagatggtt taaaatgaaa aaaccttccc
      841 aataaaggga aggttcatga agataataac ttttttaata attatagctt tctaatatct
      901 ttcatcaact tttctagtaa tcattgggaa aaatttcaag cagtagatcg cacaaatacc
      961 tacgacgata taaatgatct tcgcaaaaat agttgttgaa ccaccagcaa tcatagctac
     1021 taaatcaagt tcgaacaagc ctaccaacag ccagttcaac cctcccacaa tcaataaagt
     1081 taatgcaata gcatctaaag ttttcattag aattctcctt tcttaaatga taaaacaatt
     1141 atatatcaaa aagagaaaca ttgcttatat aacgctcagg aattttagca atctatgcaa
     1201 aaagatgaaa attttttttg ctcttccatt agtacacatt ttttagctga agctgtaaat
     1261 ttcatgtctt aatttttttt agtgagcttt tatactggat ttgtaagaaa aaagaaggag
     1321 aggatcacat atgtcggatt ggttggatat tgcaggaaaa gtagtaattg taactggagg
     1381 ttcctcagga attggaagaa gtatcgtcga aaatttgttg aagcagaatg cacaagtagc
     1441 gaattttgat gtaactgaat gccgtataca gcatgaaaac ctactatctt taaaagtcga
     1501 tgtgtcttct aaaacggaca taaaagaagg gatatataaa gtaatgaaac attttaagac
     1561 gatcgatggt ctagtcaata atgcaggaat aaatattcct tctcttctaa tagatagaaa
     1621 tcatccaaaa agcaagtatg aattatcaga acaagttttt gataagatga tcgctgtcaa
     1681 tcaaaaaagt gtatatttga tgagccaagc agttggaaga atcttggtgc aaaagggatc
     1741 aggagtgatc gtgaatcttt cttctgaatc aggattggaa ggatcagaag gacagagctg
     1801 ttatgctgct acaaaagcag cgatgaacag ttttactcgt tcttgggcaa aagagttagg
     1861 aaagaggaat gttcgtgtgg taggtgttgc accagggata ctggaagaaa ccggtttgag
     1921 aacacaagaa tatgaagaag ctctttctta tacgagaggt ataagcgtcg aacagctgag
     1981 aaacggttac agtcgaacaa gtacgatccc tctaggaagg agtggtaagc tgcaagaagt
     2041 ggcggatcta gtctgctatt atctttcaga aagatcaagt tatatcactg gagtcacgac
     2101 taatatttca ggaggtaaaa caagaggata aaggataggg gggacgcacg tgacgctagt
     2161 aaacagatgg taccaattat tgaaattact agtcactcac aagaaagttt caatccagga
     2221 agtgcaaaaa gaaatcaaaa ccagtaatca aaccataaaa aaagatattg gggagttgaa
     2281 tgaagaaatt gccggtattg caaaaatcat ccaaaaaaac aaacattttg aacttgaaat
     2341 ttatgatttt gatttttttg atgagatcat gcaaggaaaa ttgaaaacag ccagtgattt
     2401 caattcggca tccaaaagaa tcgcttattt actaaatagg ctgatcgaaa cagaggattt
     2461 cttacgtatg gatgatttgt ctgaagaagt cggtgtcagc agaggaacag tggtcaagga
     2521 tctaaagaca ttaaaagaga tgatcaatga ttttgatgta aagattacag gcacaccaaa
     2581 taaaggacta agaataatag gagatgagct agaattacga ctacttttat tttattttgt
     2641 ttctccctac ttttcagaga cgaaaacaga acagtttcta gaagaaaaac aagcaatcaa
     2701 acattttcag aagaagacga atgctagtaa acaagaaatc acccttttaa cgaaagttat
     2761 cggtatttct ttgaatagaa tctctagtaa ctatctgttg aagaagccca tcaatcgtta
     2821 cagtgggtgt tttgaatata tccaagaatt caatgaactg atcaatcagt tggaagaaat
     2881 ctatgaacta actttgagcc aatatgaaaa agattttatc agttttcctt tgaacatcat
     2941 caacacaagt ataacgatga taaggaaagc taactctgat ttgcgcttat atttcgaccc
     3001 catgatgatg cgtattcgac gattagatct cattgatctg gatgagtcct tcttatatca
     3061 agagatgaaa cagcacctct ctttattgat aaatcgggta atttttcgtt atcgattgac
     3121 tgatttattt tatggtgaga tagaaaaaaa gtatcctttt tcttaccaaa tcgctagcga
     3181 atgcatacaa gcattagaag ctatcttgca acataagatc tctaaagtag agatcagcta
     3241 cctaacgttg tatttggagt tagcatttcg atatcaaaaa gagcattttt cctctaaaaa
     3301 agtagccatt gttcatacga caggaaaagg aacagcatta atgatccaaa gacaaatcaa
     3361 aaggatattg gggagggata tacaaataac acatgtttca gaagaacaat atgaaaaagt
     3421 agatctaaac aaatatttcg ctgtttttac aacgattccg ttaaaaaatg taaatgaaga
     3481 gacaccggtg atccatttga ctaatctttt tgatgactct tggttaaggg cacaatggca
     3541 aaaagcaaaa aaaaagcgat tagccgggtt tcattctact gtttttttct atcaaatatg
     3601 gaaagaaaaa aagacgtatc aagaatattt aaacaaatta gtcgctcctt tgatcagagg
     3661 ctattatgtg gatgaagaat ttgaaaaaag gatcttgaaa agagaatatc agcaaagcac
     3721 atttgttaac aaagaatttg cttttcctca cacaaccaac cagctatctc aacacattgt
     3781 tttatcagta gggctaataa ctgatcaagg tttagcagaa acaggaggaa atggagttat
     3841 ttttttattt gccatacctg aagaaatgac ggctgaagtt gagaatgaat tatttgagct
     3901 atacgatatc ctatttgaac tcataaaaat gggaatagat gagtttattg aattagcacc
     3961 gacacaagaa tcatttttaa aactaatcaa aagtaaagga gagagacttc atgaatgata
     4021 tgtatctatt catagttttc gcagcttcag cttatgtgct gcaagcagtt atcagttgga
     4081 agcaactaaa gcatttcaat caaacatata gcgaactgcg aagaaaagga aaagtagcga
     4141 ttggtaaacg acctgggaaa atccgttcag gcacgattgt tatgctagct atagacgatc
     4201 atgatcaagt tttagaagtc agaaaaatgc aaggggttac atctttggca aaatttcatt
     4261 cattacctgt ctatgcgggt gaagatatcc attacatcga tcgttaccat ccctcagttc
     4321 gagcggaaga tcgtttgaca caagcagctt tagaaaatgc cagagaagta tacttacgaa
     4381 tagaggcagg aaatgaaaaa agtgaacaac cttcattgcc ttttattgct atgctcaata
     4441 attttcggat atggaaaagt acgatcaaag aaaaaaggag tgacaaacga tggatatcat
     4501 aacaaaattt gctgagggat ttatgaggct gttccaaaca ggtgcagaaa cattcataag
     4561 ctggatgaca ggaatcgtac cagttgtctt gatgctgatg gttgcaatga atacgctgat
     4621 tgctttttta ggtgaagacc gagtaacgaa agtagccaaa ttatcagcga aaaatccgct
     4681 tactcgttac cttgtgttac cgttcttatc tgcttttatg ttgggaaatc ctatgtcatt
     4741 tacaatggct cgtttccttc cagaatacta caaaccaagc tattatgctg ctcaagctca
     4801 attttgtcat acgagcaatg gtgtattccc ccatattaat cctggagaat tgtttgtatg
     4861 gatggggatt gctcaaggga tcgaaacctt agggctaaat tcgatggagc tggctatccg
     4921 atacttgctt gtgggactgc tgatgaactt tattggtggt tggataactg attttactac
     4981 tggatttgta tgcagacagc aaggaatcgt tcttagcaaa aaagtagagc tttcagttga
     5041 ttagagaaag gagagtttta aatgtatggt gatattaaga tcgtaagagg aaacggcggg
     5101 tatggaggtc ctttgattat taggccgaca aaagataaac acaagttcat ctatgtcaca
     5161 ggtggaggtg agaggccaat gatcgtagat aaaatagaag aactgacagg tatggagtct
     5221 atcaatgggt tcaaaacatc aatacctgat agtgaaatcg cgcttgcaat cgttgactgt
     5281 ggagggactc ttagatgtgg aatttatcct aaaaaaggga taccaaccat caatatcgtt
     5341 cccacaggaa aaagtggccc attagcacaa tatatcactg aagatattta tgtttcagca
     5401 gtagggttag aacaaataca gccactaatg gaaactgatg ataacaaaga aacagtaaat
     5461 actgcatctc cagataaaaa gtacaaatat gatacttcta aaaagataac cgagcaaaaa
     5521 gcagagcagc aaaactggat agcaaaaatt ggaatgggcg cgggaaaagt agttgctaca
     5581 ttcaatcaat cagcaagaga agcagtagat acgatgatcc ggacagtgat cccttttatg
     5641 gctttcgttt cccttttgat tggtgtgata caaggttctg ggatcggtaa tgtatttgct
     5701 aacattatgg cacctttagc aggaaatgca gttggattaa caatcattgg ctttatttgt
     5761 tcactgccat ttttatctcc tttgcttggt ccaggggcag ttattagcca agtgattgga
     5821 acattgatcg gtgtagagat tgggaaagga aatattcctc cccaattagc attaccggca
     5881 ctatttgcta tcaatactca aaatgcatgt gattttattc cagtcggtct aggtttggaa
     5941 gaagcaaaag ctgagacagt agaagtaggt gtaccatcag ttctttattc aagatttctt
     6001 aacggcatcc cacgagttat cattgcttgg gtagcaagtt ttggattata tagctga
//
LOCUS       DAZULY010000160         6002 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000160 DAZULY010000000
VERSION     DAZULY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.161"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..395
                     /locus_tag="ACG81A_002111"
     CDS             <1..395
                     /locus_tag="ACG81A_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFS5015440.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="ACG81A_002112"
     CDS             398..1228
                     /locus_tag="ACG81A_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HFS5015441.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="ACG81A_002113"
     CDS             1244..2095
                     /locus_tag="ACG81A_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5015442.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="ACG81A_002114"
     CDS             2092..2451
                     /locus_tag="ACG81A_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFS5015443.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="ACG81A_002115"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="ACG81A_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="ACG81A_002116"
     CDS             2624..2929
                     /locus_tag="ACG81A_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015444.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="ACG81A_002117"
     CDS             2929..3240
                     /locus_tag="ACG81A_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFS5015445.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="ACG81A_002118"
     CDS             3237..3410
                     /locus_tag="ACG81A_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015446.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="ACG81A_002119"
     CDS             3407..3823
                     /locus_tag="ACG81A_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFS5015447.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="ACG81A_002120"
     CDS             3823..4491
                     /locus_tag="ACG81A_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFS5015448.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="ACG81A_002121"
     CDS             4488..4865
                     /locus_tag="ACG81A_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015449.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="ACG81A_002122"
     CDS             4862..5161
                     /locus_tag="ACG81A_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015450.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="ACG81A_002123"
     CDS             5151..5309
                     /locus_tag="ACG81A_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015451.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="ACG81A_002124"
     CDS             5403..5819
                     /locus_tag="ACG81A_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFS5015452.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DAZULY010000161         5904 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000161 DAZULY010000000
VERSION     DAZULY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.162"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..335
                     /locus_tag="ACG81A_002125"
     CDS             <1..335
                     /locus_tag="ACG81A_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015453.1"
                     /translation="VLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSI
                     SLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNN
                     FYFYRNKL"
     gene            complement(461..1138)
                     /gene="nth"
                     /locus_tag="ACG81A_002126"
     CDS             complement(461..1138)
                     /gene="nth"
                     /locus_tag="ACG81A_002126"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFS5015454.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1145..1843)
                     /locus_tag="ACG81A_002127"
     CDS             complement(1145..1843)
                     /locus_tag="ACG81A_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFS5015455.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2111..3295
                     /locus_tag="ACG81A_002128"
     CDS             2111..3295
                     /locus_tag="ACG81A_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFS5015456.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3301..3744)
                     /locus_tag="ACG81A_002129"
     CDS             complement(3301..3744)
                     /locus_tag="ACG81A_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFS5015457.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3970..4257)
                     /locus_tag="ACG81A_002130"
     CDS             complement(3970..4257)
                     /locus_tag="ACG81A_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFS5015458.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4428..5780)
                     /locus_tag="ACG81A_002131"
     CDS             complement(4428..5780)
                     /locus_tag="ACG81A_002131"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFS5015459.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
BASE COUNT         1560 a         1171 c          993 g         2180 t
ORIGIN      
        1 ctgtactttt aagcactact cttaaaggaa aatttatgga aagaagctct catgcaatga
       61 ttctcacttt tttatggttg ttgttcacca gaatctatat ttttctgatg agcctgaaac
      121 cgcccaaatc cattagttta tgtttagtta taaatggtat ttttttatta tcacttatca
      181 tttttattac acctttagat agatacgtca cttatttata taatcctttg gaatactgga
      241 cttattttat aggtatattg gaaggcatta ttttctacat cgggtatttt cccaataaaa
      301 ataacaactt ttatttttat cgcaataaat tgtaataagt taccatccta gctactgagt
      361 atgtacattt actttttcat gcaaaaaaga taaacgaaac agcttttctc cataataaca
      421 agataattca tacatttatt tgcaattaaa gaaaccacgt ctacaggcgt ggtttctttt
      481 ttagcgcctt ttcttttaga cgcatccgat ttttcccatc ttgacatatt gatagcaaag
      541 gacatacctc gcacttggga tttcttgccg tgcaatgata tctaccaaaa aaaatcagtg
      601 tatgatgtgt ctttacccat aattcttgtg gtactttccg catcagtgtt tcttctactt
      661 ccatgaccgt agcatctagt ttgcagatcc tcaggcgttt tgagacacgt tccacgtggg
      721 tatctactgc gattgctggt atcccgaatg catcccccaa tacgacattt gctgtctttc
      781 tgcctacacc aggaagagac atcaattctt ctcttgacgt tggcacttgc ccatcgaaac
      841 gttcaatcag ctgctgtgca cacgctttga tgttttttgc tttatttcga tacaaaccaa
      901 tcgtttttat tttaagaatg atttcatcga ttgaagcttc tgctaacgca tcgggtgtcg
      961 gaaaagaggc aaacaaatct ggcgttgctt tattcactga tacatctgtc gcctgggcac
     1021 ttaagatcac tgcaatcaat aactcaaatg gattattgtg cttgagttct ccatgtgctt
     1081 cgggaaacat tccatacatc gtttctagag cttccattgt tttttgtttg cttaacatat
     1141 tccgctactc ctccaaccaa tttttcagtg agactttcgg caattcttct tgttgagctg
     1201 actgttctac ttctctttga agaagctgct gttttcttcg tttttggtct tcttccactt
     1261 gttgttttgt acgtagattt tttctttccc acgatagcaa aatgcggtca acgtaattaa
     1321 aactataagc ctgatttaat acggcttccc gcagtgctag cttcaaaatt tccggctgat
     1381 aatggtcttc ttctagccat tgtccgatcc gctggaactc gatcgaagaa agcgggcgtc
     1441 caaattcttg ttcaaataat tgatacactg actggatttc tttttcttgt gttttttctt
     1501 cgtatttttt ttcttgtgtt tttaaatact cacctaatag atcatagatg gggtaaagat
     1561 tatatcgatc tgctttttta ccgttgacaa tcgtcgtttc tatcttgata aatccatttg
     1621 ttacgagacg atccagtatt tgaaaaatct gatcttgttt gattcccata tccatagcaa
     1681 tcatctgcaa atcagggaaa gtatctcctt caagctgtgc catatgcaac tgtagaacaa
     1741 ataaaaattc ttcagattgt agaccgatcc tatgataatt tttaagtaaa agattagata
     1801 tgactgtttg tccagcgctt aagtaatttt ctaaattcaa catgatttct cctccgctca
     1861 atcacagtat acgtaaaaaa aacatgggaa gcaagaacag gtttcttttc tatttacttc
     1921 actgatcggt cagatttaca atgtcgtcta cttgattttt gtttgttcat gtttttgatg
     1981 cgtatcttca cttttcggaa taaataaatt aagtagattt cgctgttcct tagaatgttg
     2041 acaaggaaac aaaattacta tacactattt ttgtttccaa gttaacatta ttgcataaag
     2101 gagtttttac atgtacagtt tcacaaataa acattctaaa gtttttggat acgtctctgt
     2161 cttattcact ggattaggat ttactatcgt cagtcctgta cttccgtttt taacgctgcc
     2221 ttattctcac tccatacatc agcaagcttt ctatatcact ttgctcatgt ctgtttatgc
     2281 tctagctgcc ttcttatctg cccccattct cggtagctta agtgatcatt ttgggcgtca
     2341 tccgatcctt attatcagtt tattaggatc aagtatcgga tatttgcttt ttggtttagg
     2401 aaattcgata tggatgttgt tccttggtcg tatcattgaa ggattgacgg caggagaaat
     2461 ctccacctta tatgcttact ttgccgacat cacggaacct aatgaaagaa ccaaagtttt
     2521 tggatggatg ggtgctttag tcggtatagg aacaacgctg ggtcctatta tagggggtct
     2581 attagctgag ttggggaatt ctgttcctat atttattgga gcactcttta ctttcttaaa
     2641 tgccgtttat ggctacacat ttatgctaga aagccttcca ataaaaaaac gttcagtaga
     2701 tttaagtttc tcccatgtaa gaccttttca ccaattacaa caattattca agatttcatc
     2761 cgttattcct ttattgacag caggttttgc tgtttggttg gcagctggct ctcttcaatc
     2821 tatcttttct caattttcga ttgacgcctt tcagtggaag gctgggttgg ttggtttaag
     2881 cttctctttg attggaatct tggatactgt ctcgcaattg ttcattatgc ctcgattgct
     2941 caaaaaattt tcagaacagc agatcacaag aatagggatg ttcagtgaaa tcctcgccta
     3001 tctattcatc acattatctg gaattcttct actgccgatt ttgtttttat ttgggattat
     3061 ctgttatggt ttcggtgatt ctatttttac tcctgtattc aatggccaac tatccaattc
     3121 tgtctcagat aaccagcaag gtcttgtaat gggcggtacc caaagtatac aatctctatc
     3181 gcgcgtcatc ggcccgctaa tcgcaggaca gctttatgct gcagcaccat ttcttccgac
     3241 ggtattcgga tttttgctta tgatttttgc ctattatcac tacaataaaa aatagtagct
     3301 tcatttgcta cttcgtataa attctctgta tcgaccgata aaacgaaata ctgtgtcaaa
     3361 ttcttctttt gacatatttt caaatacagt agcatctcgc tgtacccatt tttgatggag
     3421 ttgctcgtgt ctattcctta ttttttgtcc gattggcgtc aattgaaaat agatttcttt
     3481 tttattctct tcgctttgat aagtgtttat gatccctcta gctatcagtt tttttgttag
     3541 cttactgatg gctcctctag tcatgtaact agcattcgct aattttgtca cgttaggatc
     3601 agggattttc ccgatatgtt caatcacttc tagttcattt agagtaaatc ctgcaaattc
     3661 tttttccatt tctggtttat tacgatgaat aatctcatta gctaaatcca gatattcttc
     3721 tattagcagt tgtcttttat ccatttctac accttctttt tctttcatta tattgttttt
     3781 ttgtttccta ttcaacaaag ataaaaatat tttgtaggaa aacccaatct gaaaccaaga
     3841 aaaaaagggg ttgacaaagc ttgtcataac ccctttttct gattaaaaga tagtaaagag
     3901 acagtcatcg gtaattgtga tgattttttg tcatcactta aggatgagcg gccttttcaa
     3961 aaactgtctt taaagatcat catcagtttc tagcacatat ggctgacctt catatgtgta
     4021 ctctgtattc aatgtgccgt cattttcatc tgagtagatg cgtctactga aaggaaaacg
     4081 atgagaaaca ttatctttgg cgtcatttgc tggcatcgtg taatattcct cttgcgtttc
     4141 attcaactga gagagtactt gatctgccaa ctcatcaata ttgatcgtta atttcttcga
     4201 agcactttta taatcttcta aaaatgcatc ttgttggaca aaatattctt tattcatctt
     4261 ctttctcacc tttcttttca aaatcaaata aagctgcttg ttcttttatg taacagcttt
     4321 atctttgctg cttattgtga caaaaaattc gaagaaagac aactaattgt acttttcaca
     4381 aaaaaagaca gagggtcgtg acaaaattgt gtcacaaccc tttttcttta ttgtgcagtt
     4441 tcgatttcgc ctagcgctgc ttgtgcaagt aggttcaagt aattccatgg gcggtcaaaa
     4501 tgtggttgga agaagaaatc tgaaagtgcg agatcttcta cagtcatttt attttgaaca
     4561 gctaaagaca tggtatttgc tgattgtgta atgtcgtatt tggacatgaa ctgagcacct
     4621 acgatacgat tcgttccttt ttcatagact agctccatca ttactttttc agtagttggc
     4681 atgaactctg gacgataatt atcttcaaat acagtcgctt ggacttctag atcgttcaaa
     4741 gttgcgcttt ctttggtcac acctgtcgaa ccgatcgtcc agccaaatag gtaaagtcct
     4801 gaagttcctt gagtaccacg atacgccatt ttatgatctg tcaagttatt tcccacaagc
     4861 aaaccttgac gaacggcatt tgttgccaaa ggaatatagt tttgcgtgcc acttggattg
     4921 tagttgacta ctgcagagtc tccagctgca aaaatgtctg gattacttgt ctgcatgtat
     4981 tcattcactt tgattgcccc gtttggcagt gtatctactt ttccttcgat cagttttgta
     5041 tttggacgga agcccacaca taaaatgacc atatctgtat caaatgtatc tgttggtgta
     5101 gctacttgtt tcacttttcc ttcttcatct gtgataaatt cagtaacatt ttcgcctagc
     5161 gcaagagtaa caccgcggtc tgtcaattct ttttctaaga tatctgtgaa tggtttatct
     5221 agatatttat tcaagatgcg atctaatcca tcaatcaatg tcacttgttt tccagattcg
     5281 acaaaagctt ctactaattc aataccgata tatccaccac caacaattac tacacgtttt
     5341 gcttctttcg cttgtgcaat aatttcattt gcttgattgt agtttttgca taggagtaca
     5401 tttttcgcat cgattccact aatcggtggg ataataggcc atgaaccagt tgtcatcact
     5461 aatttatcat aagaaaccgt ttctgtttct cctgttttca gatcttttgc cgttactgtt
     5521 ttattttctg tatcgacgtc tgttacatcg tgttccattt taactgttgc tcctaatgaa
     5581 gataattctt caggatttga gtagaacaag cctgctgggt cctttactac tccgccgaca
     5641 taaagtgcga taccgcaaga taagaaagaa atattgtcat ttctttcata tactgtcact
     5701 tctgcttctg gatgattttt taaaatactt tttacagctg cagtacctgc atgagtacag
     5761 ccaattacta ctactttcat ttatggtccc tccgattaac attttgtcct tcgacaaaac
     5821 tactataaca gaaataaaac ggatacaaca gtttaatgct tgtgaattca caaaggtttg
     5881 tttgtagttt cattcctttt caag
//
LOCUS       DAZULY010000162         5809 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000162 DAZULY010000000
VERSION     DAZULY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.163"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            450..1604
                     /locus_tag="ACG81A_002132"
     CDS             450..1604
                     /locus_tag="ACG81A_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFS5015460.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1577..2020
                     /locus_tag="ACG81A_002133"
     CDS             1577..2020
                     /locus_tag="ACG81A_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015461.1"
                     /translation="MGQYHYKLVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="ACG81A_002134"
     CDS             3143..4075
                     /locus_tag="ACG81A_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS5015462.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="ACG81A_002135"
     CDS             4092..4607
                     /locus_tag="ACG81A_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS5015463.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="ACG81A_002136"
     CDS             4903..5184
                     /locus_tag="ACG81A_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015464.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="ACG81A_002137"
     CDS             5186..5602
                     /locus_tag="ACG81A_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015465.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DAZULY010000163         5757 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000163 DAZULY010000000
VERSION     DAZULY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.164"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            136..975
                     /locus_tag="ACG81A_002138"
     CDS             136..975
                     /locus_tag="ACG81A_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HFS5015466.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            1126..4143
                     /locus_tag="ACG81A_002139"
     CDS             1126..4143
                     /locus_tag="ACG81A_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5015467.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            4207..5670
                     /locus_tag="ACG81A_002140"
     CDS             4207..5670
                     /locus_tag="ACG81A_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5015468.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
BASE COUNT         2202 a         1002 c         1196 g         1357 t
ORIGIN      
        1 tccttttagt tattttagta ccaaagttag tatatcaaaa aataaaaaaa tctacaaaaa
       61 atatatcgac tgatagatat aagtttggta ctatgttttt gtaaataaat ttattcaaat
      121 cggaggcgaa aaacaatgaa aatgttgaaa gtattggcac taggaatcgt agtcggagga
      181 gttagtcttg ctgtgggaac gaatgctgaa gcagcagaaa acaaagtttg gacacctcgt
      241 tcggcggaac aaatcaaagc agaaattaaa gggaatgaat acacaattgt ctggggagat
      301 accctaagcg caatcagtca agcaacaaat attactgtcc aaaaattagc ggacatgaat
      361 aagattacaa atgtggattt aatctatgca ggaaataaat tagtatttaa tggcaatgta
      421 gcaagtgtac aaaacagcgc aggtgaaact gtggctcaaa cagtgattca agagaaagac
      481 aaagttgatc cgaatcaacc agtaggacaa ggaacgactt caaatacaca aacgacacca
      541 aatacaacga caccaacaac accagctgga gatcaaacag gaacaactgg tggtacaaca
      601 acgccaagta caccagaaaa caatggtggc actactcaaa cacctacaca accaggtaat
      661 ggtggaaata ctggaggaga aacaactcct tctaacccaa gcacaggtgg aggaacaaca
      721 acgcctgaag aaccaagcaa tcctggtgga ggcgatcaag gcggaaatgg tggcacaacg
      781 acacctgaag agccaacaat tgtagttgga gagattggta atagtggaaa ggtatttaac
      841 actgtagatg aagcctatga ttgggctaat gcagaggtta gcgatccaaa ttcgcagtgg
      901 tacaacaaag gttttatggt ggatgttatt atttacagtg acgggtcaca aaaacacact
      961 gtagatttct actaaaaata aaagaaaagt aagtggttga tgaagactag aattataagt
     1021 tctagtcttt ttttgttttg aggaggataa aaatgtttcg gtgtcgaagt ccataagaaa
     1081 ttactttaga agactgaaaa ataaaatgaa aggaacgaaa aaaatatgtt taaaagaatg
     1141 gcaacttggt tggcaatttt cgttacattt ctcaatccca tgttaggatc tgtaacggca
     1201 tttgcagaaa cgataaatga atcaaattct actgaagaag taatgaagac tgaagaatca
     1261 aaaagtagtg aagaagcgat cagtactgaa accacaagtg aaacaacaga agagacaact
     1321 acgcaggaca cgacagaaga tgtttctccg cccaatgtaa tgactcaaga ggtccctgaa
     1381 ggttatgaga aatactttat aaccattgaa gatattcaaa aagcgacttc tttttctgaa
     1441 caagaacgat tggcgcaaga atctgaagaa tggcaagcaa tcttggaaaa gaatgcacag
     1501 acacgtgcta ccaatctaac attgactgaa aatggtcgta aagatgtgaa tatgactcga
     1561 attactcctg aaggaacaat gtatactcat gattacatgg ttcgctggca aattgacgga
     1621 gaagatgttt tttgcattca agaaggcgca atcacgagtg cgggtattca atatgatgtg
     1681 acaccactcg aaaatttaat tacgacaagt gctattagaa aaaagatttc tttaattggt
     1741 tattttggct actattctag tggttcaatg gataactatg tattagcaca aacatatatt
     1801 tgggaagttt taggtggttc atttgttaat tatggaacta ttggacaatc taattatgaa
     1861 gctttcaaac agactgtaga tagaaaaata aacaatttac ttgaagatcc ttcttttgat
     1921 agccaaacag tgacggttaa agtaggggaa gaagtagaag ttaaggatac aaaaggtgtg
     1981 ttttctcaat gggatgggtc ggttgttgaa aacacagcta acgttcaagt aagaaaatca
     2041 ggaaatagtt tgtttataaa agcaaatcaa aactcaaatg acggacgaat ttctgtttct
     2101 tcactggatc caaattctag aggggtaggt tcaagttttg cctatgtaga tcctgttgct
     2161 caaaatctag gaaagcttta tgtgccagat cctaatagag ctggactaac tattaaagtt
     2221 ctaaaaaatg gcgacttcaa actaaccaaa ctagacgctg tcacagggaa aaaagttcca
     2281 aatgtgacgt atgaaatcaa acgtcatgat acgggagaaa caaaacaata tacaacggat
     2341 gccaaagggg aaatcgcctt aaaagaaatc cctgcattct atgaaaaaga cggaaaatat
     2401 gttcaaaata aattggatgt caaagaagtc aaagcaccag ctgggtatat tctagacaaa
     2461 caaactcata cgattacgat tgaaccaaac aaagaagtga cgttaactgc aaaaaatgac
     2521 ttacaacagt tcattccaga aggaaagaaa gtcaaagaag tctataatgc ggaagaatcc
     2581 tggaatcaag gcaaacctgt ttatgatacc gttcctgcag aaaatatcga atttgatatt
     2641 atcgccaaaa cagatattac tgcgccagat aaagaaacag tccttgtgaa aaaaggagaa
     2701 gtcttggatt cagtaaaaac tgacaaagac ggacactgga aggcaaacaa aaaattctac
     2761 aatggcgatc aaaacaaata ccaattgatt gaaaaaaatg tgccagacaa ctaccgtcaa
     2821 ccttctgaag aacaaaccac gttctctgtg gcctatggcg ataacacgac gcctgtgctt
     2881 acgtttgaat tagatgtgat tgacaacttg ttaaaaacgg gtgaagtaga cttcaataaa
     2941 gtcgattcaa tcaatcatct acaattacca ggagcgaaat tcctgattga aggtatgact
     3001 gcacataata aaaatgtgtc ctttgtgtat gaaacaaaag aagaaccaga aaaactaaaa
     3061 ttaccagacg gtacgtataa atttacggaa attgtgttcc ctgagcatta tgggcaagcg
     3121 tcaggacaaa cagaaacgat tttagtaacg gtcaaagacg gagaagtaca accaattact
     3181 gtgaaaaacg aagaaatcgt ggttccagaa gaagaccaac cagaaatttc tactttgttt
     3241 gtgacaacga caggtggaaa agatttcaac ccgaaaaaag accaagaatt agtggataaa
     3301 gtgtcccaag tattccctaa atctgcggtt gggacagaga aatattgggt gacgcaattc
     3361 cataaaatta ctgccgatgg aaaagtcact gtattagata cggtaaaaga aacacgaaaa
     3421 gtgaccgatg aaaaagaaac atttgatgtg acgttcaact acaaagcaga catggtttca
     3481 gacggtgaca cgattgtcgc aacgcacatt gtctataacg acaaagaaga ggaagaagaa
     3541 ttcgctcgtc attttgattt aaccaataaa gatcaaacag tgactgcgaa agaaccaaaa
     3601 atcgaaacac gctttattac agtgaatggc gaccaaacat ttgatccaga caaagaccaa
     3661 gagttgatcg atcatgtatc gcaagaattt ccagaatcta cggtagggga aaccaaatac
     3721 tgggtgactc aattccataa gattgataaa aatggaaaag atacagtcgt tggtacggta
     3781 gaaactgcac gtaaagtttc taaagcgtct gaagagttta aagtatcctt tgactacaaa
     3841 gcaggcacgt taaaagacgg ggagaaattg gtcgcaactc atattgtcta taaggataaa
     3901 gaccataaag aggaagaagc acgtcacttt gatttgaaga atgaaaaaca aacattgaca
     3961 gcaaaaggca ttaaaaaaga aacgccgaca acccctgtca aacaaacgcc gacgagcaca
     4021 ccaaaacaaa gcttaccaaa aacaggagaa acagtgaacg atgtgttaat tgcattggga
     4081 ctgtttcttt ttgttggtgt gggggctgtt ctggttcgtc gtaaaatgaa acaaagcaat
     4141 taaagtatga ctagagacag caatcagctg tctctagatg attgattgaa gaatggaggc
     4201 aatcttgtga aaaaaacgag taaatgttta tctgctttgt tattagtagg tcaagtattt
     4261 gggtatacgc cagcggtttt ggctaatgaa caaggcaaca atcaactgac aaattcgcaa
     4321 tctgcaacga tcactgaaga aacgacaaca aattccactg gagaagaaaa tcaaccagaa
     4381 acaccgactg aagatacaat agatacacca gataatccag aaatgaacgc tccaccaaca
     4441 ggttctgaag aacaaccaga aacaccgaat caaccagaca aaccagaaga caatcaacaa
     4501 cctgaggagg aaccgtcaga agaaccaaat gagcaacctt ctgatgaaag ctcacagact
     4561 cctcaaaaac caagtaccga aacagaagcg gaaccgccaa caaaaacgga acgcccacaa
     4621 aaggcacctg aacagagtgt gactgtacct atgaatgacg taccgcaggc acaatcaatc
     4681 ggttcagaag agacaaaaaa ctaccaccaa gcaccaagcg caccgataca agaattagtc
     4741 ccttccagca cacaagataa cgaagggact attcattttg aaaaagacgc gtctgtagaa
     4801 acgtttatcc gtaaaatcgg tgaatctgct cgtaaaattg gacaagaaaa tgatttatac
     4861 gcttctgtta tgatcgcgca agccatttta gaatccgcaa gcggacaaag tcaattggct
     4921 caggcaccaa actacaatct atttggaatc aaaggcacac ataacggaaa gggcgtctct
     4981 tttgcgacac aagaagattt aggcaatggg acactttata cgacccaagc aacttttcgg
     5041 caatatgaga gctatgaaga aagcttaaaa gactatgcca aactgttaaa agaaggctta
     5101 gttggaaata cctcttttta tgatggtgtt tggaaaagta aggcaaaaac ctatcaggaa
     5161 gcgactaaat ttttaacagg aagatacgca acagacacta gctacgataa aaaattaaat
     5221 gggttaatcg aaacctatga cttaatcaaa tatgataaag aagtagctgg tccacaacta
     5281 agcaaagaag gctatatggt acctctaaaa aattattcaa tttctagttc cttcggttgg
     5341 cgaggagaag aatttcatag aggattagac atggcagcca atcaaggaga accaatctat
     5401 gcgagcaagg caggaacggt catagaagct tcgtatcata ctttgtcagg aaattatgtc
     5461 acaattgagc atgaagacgg aagtgtggct ctttatgcgc accaacagga atattgcgtg
     5521 aacgtaggac aaacagttga acaaggacaa attattggtt atgtgggttc aacaggaaat
     5581 agtacaggac cacatttaca tttagaactt gccttagatg gtagtttaac aaaagacaaa
     5641 ttaatcaacc cagaaacagt cttattctaa agaataaggc tgtttctgat aaggagtggt
     5701 atagaaaatg tggttacttt ttaaatattt gatatatttg tatctctcat tttcgtt
//
LOCUS       DAZULY010000164         5749 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000164 DAZULY010000000
VERSION     DAZULY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.165"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1128)
                     /locus_tag="ACG81A_002141"
     CDS             complement(<1..1128)
                     /locus_tag="ACG81A_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS5015469.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
     gene            complement(1164..1874)
                     /locus_tag="ACG81A_002142"
     CDS             complement(1164..1874)
                     /locus_tag="ACG81A_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5015470.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            complement(1942..2757)
                     /locus_tag="ACG81A_002143"
     CDS             complement(1942..2757)
                     /locus_tag="ACG81A_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFS5015471.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            complement(2768..3736)
                     /locus_tag="ACG81A_002144"
     CDS             complement(2768..3736)
                     /locus_tag="ACG81A_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5015472.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            4499..>5749
                     /locus_tag="ACG81A_002145"
     CDS             4499..>5749
                     /locus_tag="ACG81A_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5015473.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT         1772 a         1183 c          915 g         1879 t
ORIGIN      
        1 attttttata acagttgaca tgtaagcctc ttctaacgtt tgattcaagc cgagaatttg
       61 tggatatacc tctgctacta cttgtccttc gttcattttt tctgctgtct tttgagtaac
      121 catttccaaa tcactaatcg aattaaaaac gccttcaggt aaccgctttc caaattcgac
      181 aagaattgac gcagctttat aacccgcttt atctccttcc gttgtcaaac agcaaggaat
      241 ctctacttca ttgaatgcag caacttctgc tttaattgcc tcatagtcaa tctcgggtga
      301 agctactcct gactgataaa caccgtaaat atctccactt ctatccgcaa caacaccatc
      361 taccatgatc ttcactccac caaagcggac cttttcaggg aaaacttttt ccttatactc
      421 atatagaagt tcatgctgta gaggttcttg gactgcctgc acatataggt tactaagtat
      481 tttcctctga ggtatattag aaagaaattc tgcgtcatca aaaacgattt ctttcattga
      541 tataacacct tttgaaagca gtaatgcttc aaattcagcg taaacctctg ctaaaagctt
      601 tttgtttgct agcatttcct taattaataa tactcgactt tcagcactgg tttctttttc
      661 tgtaaaacca taagtctgct tagcaatagc attcatccaa caatggctgc gatttttgtc
      721 gatcgctgtg attggaccaa agtggtttat ttcattcaat agctcttctg atgggtcagt
      781 tttccacagg gcagcatccc agccatgagc ataaattggt tcatctgctt ttttatggtt
      841 cattacagct ttaatacgct ctacagcctc gttagctgaa gatgtatcgc ttaaatctat
      901 gccagcattc atagccaaat aaccactgaa aaatacatga ttatcatgaa ttgctggcat
      961 aacgaattga tcatcataag caattaattc cgtgttttca tttaatagat cactgtaatt
     1021 ccagttgttt tccacctttt caatgatccc ttcattgatt acaatataac ctttgaaagg
     1081 tggctgctct ccatcaaaaa tcatttttga ccgcaatatt tgcttcataa ataaaatcta
     1141 ctccttgaaa tccaaacagg aaatcataag taaaaacgat atacttctcc caggtaatat
     1201 tgattaccta catgaattgg tatgttgttt tgatcgttga ttaaagtatc cataaagaaa
     1261 aagggcgtcc caacagctac ttccattatt ttggcaattt ggtcatcagc tgtgacaatt
     1321 tcaagtgcag tttccccagg attgatggct aaggtacctt ggactttaag tacttcatac
     1381 aaagaatcag tcagctcttc ctccattaag aaactatatc tttcataaga aaaataattg
     1441 ttttctaaca taatggggat gttatccgcc attcgctttc tctggatata taaaacatca
     1501 tcaccttgag cgatatcgag tttttttgcc atcccttcag ttgctggaat gatcttgcgt
     1561 tccaaaacaa tgctagtcgg tgtaaaacca ttagatttac aactctcact gaaacccata
     1621 atatattcaa ttttacgaaa aactttatgc cggttgacaa aagttccttt cccttgtttt
     1681 ttaagtaggt acccttcttc taccagttct tctactgctt ttctcactgt gattcggctg
     1741 acctcataac tttctatcag ctctgattca gcgggaattt tcgtattctc gggatactcc
     1801 cctgtattga ttttattttt taatatttct tttaattgta agtacaatgg taattgtgca
     1861 tttgtatcca gcattttttt aacctccact ttaccgggat ttctttcttt aggttatcaa
     1921 atgcaggaat ttaaaacaag ctcaatcgta atgaatacca aagccgaacg aaccgtcaac
     1981 cagacaagac ttcccagcaa attcattccc ctttttcatc gcggtgacta tatcgtcccc
     2041 ttcgagaaaa taaagtagga aagctgttaa gaaagaatct cccgctgcca ttgtatcaac
     2101 tggttcttct agcacggggt tcacttcata aaactttgct ccatcataac caatagccgt
     2161 tttttctcct cgggttgcaa tcaatacatc atttcgctca caaaaatgct tcatcaaaaa
     2221 ctgctttact tcttcatcgg aaaagtcttc tatagaaaag aagccaaaat caactttatc
     2281 aattgcccgc tgaacctctt cttccgtaaa gtcgtcagaa aaatcataag aaatctttcc
     2341 agaaacattt tgtaactctt ctaatagatg attgacatgg ctatataatc ccatatgaat
     2401 caattcaaac tggtttaaat attctctgtc ttcttcagta agctgaatcg ggtattcacg
     2461 agtaacacct cccgaattgc tgccaaggaa aatccggtcg ccctcatcaa tcttcactct
     2521 ggcacagcca ttctcgcctt tttcaaagcg agagtgactg ttgtccagtc ctatttcttt
     2581 tattgctgat aaaacatgag ctgcctcctc atctgagcca aaaactccca taaagctact
     2641 ttcatgtccc agtttttttg caaaaactgc aaaattcaat gaatttcctc caggatacat
     2701 aattcccaga ttttcatatt tatcgacgac gttatcacct aagcctaata ccttcatttt
     2761 aatcctccta gtactccact ttccccatat agcgacgaac atctaaatca tgattgcgta
     2821 catctgctaa agctgaacga tattctgaaa gaatactgta gaataaaatg ggattgaaga
     2881 attcagaaac ttcatctgat atcatatcca atcctagttc ctttgcatca acgatttcta
     2941 ctctttctgc atatttatca aggaatcgtt ttgcccgttc atctaaagca cgagtgcgtc
     3001 caatgctcat caagagaata aaattcgtat ctttgtctgt tacttcaaaa ggtccatgga
     3061 agaattctcc agaatgaaca gaagttgaat caagccattg catttccatc aaagagcaaa
     3121 ttgaaaaacc ataagcatgt ccatatgatg gtccactacc taagatataa aacattgttt
     3181 catctttgta cttatcagca aacactttaa tacgatcctg cacctgctta atgccatttt
     3241 caattacggt atcgataacc ttcatgcctt ctctgaattt accgtaatgt tcaaatgttt
     3301 cggtaacatt gaccaaagat acagttaaat caagaatgat ggccatagga ttattgacta
     3361 tttttgcatc atctccccat tcgtaaagga aactatgatc agaaaccttt aataaatccg
     3421 cagttttatt atgagtcaga ccaacagtca cagctcctct agcttgagcg acttctcctg
     3481 cttttaccgt ttctgctgta tttcctccat gagaacatag aatgacaaca gatttatcat
     3541 ctaattgctt tggggttaca tggttaaatt cgttcgctgt atataagcct gtaatacact
     3601 tcgatgcttc ggctttaaga aaataatctg agacatacat atctaccagc gaaccgccac
     3661 aagccaccaa ataaattcca gaaatctctc catcattttt ttctacaatt gcgttaaccg
     3721 tttttgctac atccatcaac atttcctcct taaaatttta aaaatgttaa ttttatccaa
     3781 aataaaagta actgattaaa tgtaaagata tcacataacg tcatataacg tcatgtgaca
     3841 taattgcatt gtatccgctt tctttgtgtt tgtcaatagg aaaaatttat ttctctaaaa
     3901 aatataaata atctgcagtg aaacttgagt acatatacta atttcgaaaa atgcctatga
     3961 tttaaataat tatggtgctg ggatgaaggt ttataagttt gtcttgattt gtttagaaga
     4021 aatcgttttt gcacacagtt gaattaggaa actactggag tggaaaataa agtgatttga
     4081 cgggtagatg ggtgcactta aatttgaaaa tggcttaaaa tgctttattt tgaattgcag
     4141 aatttctttt agtacgcaat ttatggtctt aaaactgatt aaaaagtgat acattgattt
     4201 taatacaatt tatgatacaa aaaatgacct cctttcttaa ataaaagagg tcattttgtt
     4261 ttgtctcata atcgattgtt tttgatacag tagttatatt cagaaaaaat aaagactctt
     4321 ctgagtttat aatcctttgg attatatacg gatacagatg tagacataaa acaagctcaa
     4381 ttttcaaaaa tggcattact cacctttttg gtgaattatt tgtataaaaa aaatatagaa
     4441 aagacaccct gaaatccttt agaattaatt tggctaccaa ttcataggag gattcaagat
     4501 gtctcatact cagcttatca aagataccct aaatattcta gacctaaata tctatttcga
     4561 ggaaaattgt ttaactaaag aaaaatacaa agggcaaatt tgcttgattt acaaaggaac
     4621 tcttctgtac aagccagaag agtgttccca ttgtctttgt gtcgtcccct ctaggatcat
     4681 tcgttggggg acgacaaccg ttcggttgtt actgaatgat gtgtctgagt atcgcactta
     4741 tctggagctg aagaaacaac gatttaaatg taagtcttgc caaagaacat tcgtagcgga
     4801 tacctctgtt gctgaaaaac actgtttcat tagtcaaaaa gttcgttggt ctgtcattgc
     4861 tcgcttaaaa gagaatactt ccatgacgga aatcgctcgt cagaaaaata tttccacttc
     4921 ttcggtctat cgtgtcatga agcgattcta ccgcccattg aatcctttca aacagacttt
     4981 gccaaaggtc ttatgctttg atgaattcaa atcggttcga agtgttgcca gtgcaatgag
     5041 ttttatcatg atggacggac aaagccatgc attattggat attgtcgaaa atcgacgctt
     5101 gccttattta gaacgctatt tttctcggtt ttcattagcg actcgtgaag ccgttcaatt
     5161 gattgttatt gatatgtatg cgccttatgt ttcgttggtg aaaaagcttt tcccgaatgc
     5221 tcagttgatc atcgatcgtt tccacatcgt ccaacatatt ggcagaactt tcttgaatca
     5281 tcgagtgaaa gaaaccacgg tacgacttaa aggaaattcc cactctcaac gagggttagg
     5341 gaaaaaattg aagcgctatt ggaaactcct acaaaaagaa gaagcaaagt taaactatga
     5401 aaaacgggtt tggcgtgcca gttttaaaga ttatttgact gaaagtgaga ttgccgatcg
     5461 actgttagct gcttgtccca acttacgcca aggctatcag ttataccaag acattcttta
     5521 tgcggtaaag aaaagagatc agtcactatt tgaggattgt ttaactagag acattagcgg
     5581 tctgccagaa acctatacaa ccactttacg aacgtttaaa aagtttctgc cacagatcca
     5641 aaatgcgctt cactacagct attcaaacgg accattggaa tgcttaaaca accacatcaa
     5701 agtattaaaa cgcaatgcgt acggctttcg caacttttac aatttcaaa
//
LOCUS       DAZULY010000165         5732 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000165 DAZULY010000000
VERSION     DAZULY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.166"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..1171)
                     /locus_tag="ACG81A_002146"
     CDS             complement(41..1171)
                     /locus_tag="ACG81A_002146"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFS5015474.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1230..1457)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1819..3021)
                     /locus_tag="ACG81A_002147"
     CDS             complement(1819..3021)
                     /locus_tag="ACG81A_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5015475.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3081..3284)
                     /locus_tag="ACG81A_002148"
     CDS             complement(3081..3284)
                     /locus_tag="ACG81A_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFS5015476.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3426..4493)
                     /locus_tag="ACG81A_002149"
     CDS             complement(3426..4493)
                     /locus_tag="ACG81A_002149"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS5015477.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4551..4658)
                     /locus_tag="ACG81A_002150"
     CDS             complement(4551..4658)
                     /locus_tag="ACG81A_002150"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5015478.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4821..5633
                     /locus_tag="ACG81A_002151"
     CDS             4821..5633
                     /locus_tag="ACG81A_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5015479.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1687 a         1182 c          913 g         1950 t
ORIGIN      
        1 cgactttttg ggacgctttt ttttgccaag cctcacattt ttatagccaa acttcttttg
       61 cgacttgctg caataattgg attttcttcc attgttcttc ttctgttaaa tggtttcctt
      121 cttctgttga agcaaaccca cattgaggac tgatacttag attttccaat ggtacaaatt
      181 gttgtgcttc ttttattcgt tcttttattt tctccgtatc ttccaaatct ggaaacttag
      241 aagtaactaa tcctaaaaca actttacttt ctttgttact ccaatgtctt aaaggagtaa
      301 aatctcctgc tcggtcagaa tcgtattcca aaaagaaacc atcataattt gttgaaaaca
      361 actcttctgc aatgggttca tatccaccgg aaaacagcca agaagaagcg tgattcccgc
      421 ggcagatatg tgttgtgatc gtcaaatctt ctggtttgtc ttccagtgcg ttattgataa
      481 tctctgtaca aatatgggcc atttctttcg gatcaattcc tttttgaaga agtgcttctc
      541 ttttagtttg atcagctaga aacgcccaat ttgtatcatc gaattgcagg taacggcaac
      601 ctttttcata aaaagcatga atcgctgctt tgtaagtaga ggacaagtcc tcataaaaat
      661 cagatagatc aggataaatc tccttgattt ttgaagaacc atcattagat aggatctctt
      721 gtcgtaaaat catcgtggga ctagggatcg ttgcttttgc aacaaaagta tcatctgttt
      781 ccactacttc tttcagaaaa gcaaaatctt ttaaaaatgg gtgatttgga ttgaaagata
      841 atttatctac tacccgaata ttataagctg gtgctgcttt tccgtggaat gtttgattat
      901 atccagtttc tggaacatag ccttcgatac catttagttc ttctaaaaaa tcaagatgcc
      961 aaaatccacg ccgaaattca ccatctgtaa ttcctttgat gccgactgct ttttggtctt
     1021 ccactaattt ttttatttcc tggtcttcga ttgcacgaag ttcttctttt gaaagtaagc
     1081 cttcttgaac tttttttctt gcttctttga tcgattgcgg gcgtaagaaa cttccgacat
     1141 gatctactgt aaatggtcta tttttgctca tttcactcac tcttttcttt gttttttgta
     1201 aaaaaaatcc ttttactagc aaatactagt aaaaggacga ttatactcgt ggtaccacct
     1261 tttttcagaa aaaacattct ttctctcagc ctgacaataa aatcagtggt cttggtaacg
     1321 aaagactgtt cgtttttgcc tacctttcga caaaaaatcc tctaaggcca ttttcgacag
     1381 tcaacttcta gctttttaca ccaaccaaag cctctcttga accattttct gtctactttc
     1441 cttctcatcg gatactatta gaataaaatt agaatagcat gtttgaaaga aaagtcaata
     1501 gggaataaga acaaacaaaa actaatttgt tattcttagt aatttgtttg tggaatacaa
     1561 taaagctttt ctaatatgat aattgttcag tcgtcctttc atcttagatc acttccttaa
     1621 aatgatcata cccgtttcaa ccacataaaa aaggacttaa ttttgaaaaa tttcccatca
     1681 aaactaagtc ctttttatta tagttatgca aatttgctta ttttctcgct atttccgata
     1741 catcttgaac aggagcaatt cgactgaaaa cactggtgca aaagcattta tttgtcttat
     1801 ggtagaaccg tggaaaactc agactggtaa taaattgtca ttttttgaaa gaatagcgcc
     1861 ctcttgaaca ttatcaatcg cacttaccac ttgttgatct gactttgcat ataagtgacc
     1921 gtaagttcct aaagtgattt gaatattcgc atggcccaat cgttcttgaa tagatagcgg
     1981 ttgaatattc tgctcaatta gaaatgcaac atgagaatgt cgtagcgcgt gaagcttaat
     2041 cggtttgaca cccgcttttt tagcagcttc ttttaaccag ttcgcaaaag aataaggtgg
     2101 cgtaaatgtt ccgtccaact gagcaacata ttcaaaattt ccaatacgtt tttgcaagtc
     2161 ataccactgt ctgagtttta aggaaagctt cttccccaac ccaatcgttc gataagcaca
     2221 tttagttttg ggagtcgaaa agtaccaatc atcttttgta ttataaatca atgtcttctc
     2281 aatcgttatc gtgtttttct caaaatctac attcgaccaa gacaaagctt caagttctcc
     2341 aatccgaatg ccagtataaa agagcatttc atacacaatc ttccgatggt gggtttgaat
     2401 cttatcatcc ttcaattcat tcatcacttt ttgaaactca ctgtgttccc aaaactcaac
     2461 tttcttattc tcagaaggaa gcgaagaaac atcttcaacg ggatttttag caatcacccc
     2521 ctctttaaca gcccgatcca atacaatttt taggtgccct cgaatccgat tgatataaga
     2581 ttttgataac gtttcgccat taactttttt ccgtgaatta cgtgactctg tctggcttag
     2641 ttcaaataac caatcctcaa tatcgtgact tgtaatatct cgaacacgaa aatgatagaa
     2701 aaactgcata cgcttttcga aaatgaattg cgcattctca tatgtttgac ttttgacctg
     2761 tctctcgtac cattttacaa acctatcctt gtaaaactct tcgaaagtca tttgattggt
     2821 agaacacagt tgattaggaa ttgtcttgtt aaaatcatgt gtttccatgt acttgtcata
     2881 agcaattttg gcatcttttt gtgaagcata acctcgttta gtcttctgaa cctttcttcc
     2941 attttcatct atacctagat acgctcggaa gaaccatttt tttgtctttt tatcttggta
     3001 aatattttta atttttggca tcgttccatt cctccctgta cttgagaaaa gctactttaa
     3061 tagtaccgcg cttgtactgt ttattccgca acttgaatgt gaaaaagatg ttcaattact
     3121 cgggatggca ccacaccaac ttggcgatta ttataaaaat caataccttc aatccttgca
     3181 agatatgcct tgctctcttt aatcattcgt gcagcttgac taggtttaaa gcccatctct
     3241 atcaaaattg cacgatttac catgaaatca cattttgttt ccattctctc acctcctgat
     3301 tctttactcc ttttaaaata atcctcttta taatctattg tttctcattc gatggttgtg
     3361 ttatactcgt cttgtcgagg cggagtacgt tgcacaactt gttgtgtaag cagtactttg
     3421 cttttttagt agcttgtttt ctcatttgat atactttgta attttcgttc ataatactca
     3481 acctcctcct gataatactc cgggtctgcc ttcatatttt ggagaagttt gatctgtctt
     3541 ggattcattt ctccttgttc aataatcaaa ggcaacaagg aagaatgaaa cgtttgatcg
     3601 acctgtgcaa taaaaactaa tgatggtgaa acaccatgaa ttaaccaact catagttttc
     3661 tcaaaacttt gaggaattgt atctaaagaa atggtgattg cgccataatt gctaataaag
     3721 gctgcccatt tggcatcaac ttttgcacct agatcatttg gatcacggtc ataaaaacaa
     3781 attgctccat taagtaactc aaagaacagt ttggttaaat catgggtttt tgaaataatc
     3841 tttgccaagc tttgcgcttt ctcttttcga taccgtagtt caaagcgatt ctttactttt
     3901 gcctcttcta aaggaattcc tcgtcgcttc cgttgttcat ggtccttctg ataaaagcaa
     3961 aaacgacact gactcttctt tgagcctaaa tacaaggtct caccatttga ttcattgttt
     4021 atgatatccg taccgccatg agattcactc acttgaaaag tggtccacac atggcctagt
     4081 attaccttat gcttaagttc tggaatgctg agactcccca caaaatcatc taaagtaaaa
     4141 tcaatccgtg taaagttccc ctgataatct aataccttac gaaaaaactg ttgccaattt
     4201 gtttttcgtg tttttagcca tcttgctaag tgtttacagc cttgacctga aaactccatc
     4261 acagttcctt taaccgcatc gtcaatggaa taccgaatcg tgattacttg attccacacg
     4321 tattggccaa tatagccctt tggcgcataa tccagttcct caaaacgttc aatgggcatt
     4381 tcaataatct cttcaattag ctttaaatgt tgattcgaga aaaagtgaat ggttagataa
     4441 tcaatcaaaa cacggttaac tggaacgacc tcttcttgca aactatccaa cacaagattc
     4501 aatttttctt cacttaattg tctcgttcct ttttctactt tcgataataa ctatctcgaa
     4561 atccccactt tttctgctaa atatcgttgt gtccaccccc tttcttctcg agaaactcga
     4621 atggcttcac cgagatcatg ttgattaact tggttcatcg acaaacctcc ttctgctatt
     4681 tacttgtcaa agaacctatt gaacttattt taagtacatt tttattgtac gcctgtattt
     4741 attttaagtc aatatgtttt gacttttttt aagtacacac gtatactaaa tttaagaact
     4801 tatcctgagg aggagttttc atggagatag acaagcaagc agtcggcaat cgaattcggc
     4861 agattcgcca agaattaaag ttgagtatgg agaaatttgg caaactaatt ggtgatttac
     4921 cgcgaagtac agtcaataac tgggaacgtg ggatcaacct tccaaaaacc gaaacccttc
     4981 accaaatcgc agagataggt catgtcacca atgaatatct tttgtatggt gatcaagaga
     5041 atcaatacat tttagaaatg ctccaaaaaa aggcaggtaa gcttgatcct aagattgaag
     5101 gtctcatagt agacgaaatc aaacacgctg accttgttag tgaaaaagaa atggatcgaa
     5161 tgattgaatt ttttattaca aatctcattc caccaactga acaagaccag tttactttcc
     5221 aatgtatcga tgaaaaaaag cagctttatc ttggttcaac taattttggt aaggaagccc
     5281 agctctattt gcaacatgat aatcaaaatc atattctgca catgatgccc tttaccttct
     5341 ccaactttag tgtggatcgc atactcgtct atttagcaaa tcaaaaatcc tattcctatt
     5401 ttgggaaaca tttacccaag gaactaggtg agaaagccat ccttctatac tcaattaatc
     5461 aattgaccag cgatgtgcga attgccccgt tggtctattc agaagaaacc gcttcttatc
     5521 agtacacaga ggacaatcaa tatctactag aacagcaata tctttatcta ccattcgtga
     5581 tggaagtcga gaaagaacgc ctgttaaatg cagcgtatcc atcatcaaaa tgaggtgaca
     5641 aaattgataa gcacgtgaca aagaaaatac tgctaggaca ctccccaatg ggtagttgtc
     5701 acagtttcct gttctttgtt agccccctgt ta
//
LOCUS       DAZULY010000166         5710 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000166 DAZULY010000000
VERSION     DAZULY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.167"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(131..1177)
                     /locus_tag="ACG81A_002152"
     CDS             complement(131..1177)
                     /locus_tag="ACG81A_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFS5015480.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(1188..1460)
                     /locus_tag="ACG81A_002153"
     CDS             complement(1188..1460)
                     /locus_tag="ACG81A_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFS5015481.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(1495..2403)
                     /locus_tag="ACG81A_002154"
     CDS             complement(1495..2403)
                     /locus_tag="ACG81A_002154"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5015482.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(2535..3767)
                     /gene="pepT"
                     /locus_tag="ACG81A_002155"
     CDS             complement(2535..3767)
                     /gene="pepT"
                     /locus_tag="ACG81A_002155"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFS5015483.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(3782..4903)
                     /locus_tag="ACG81A_002156"
     CDS             complement(3782..4903)
                     /locus_tag="ACG81A_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFS5015484.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(4900..5601)
                     /locus_tag="ACG81A_002157"
     CDS             complement(4900..5601)
                     /locus_tag="ACG81A_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFS5015485.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
BASE COUNT         1644 a         1233 c          979 g         1854 t
ORIGIN      
        1 cgacttatat aaatcttaaa aaaacaaaat tgattgtatg tatataaaaa aagagccaaa
       61 caaacgtaaa attatcttat ccataacgtt tttctttgat ttgataaaaa gggtagatct
      121 tataaaagac ctacccttga ttttctttat tctctttttt tacttctatt atttcttctt
      181 tgatcactct tcgccgttca gcaatcacat acacaaatcc atagagaaat ggcaagataa
      241 aggaactgaa acgatacagt aaaagcgaag aaactgcgtc aactgttcca acaactggtc
      301 tgaataataa caaatagaca aattcaaagg aaccaatacc agcaggcgta ggaataaccc
      361 ctgaaagcac tactgcaaag ctgataaacg aaatggtcag tagaaaatct attttaggat
      421 ggttttcaat cagaatcaaa taaggaagta cataccaaaa tacaagtttt aacatattcc
      481 atagataaat ccgcaatagc gcagtcctgt cttggataat ggtttggacc gtttccctta
      541 aagagtagat ctgctgattg catgtatcca cccatttccg caaacgctca cttttaaata
      601 acttattcga taagctgaca aaaaatattt gaacattcaa gctgacggac atcgccaata
      661 aaaaggcaac aatcacgatc gtcaaaagga taccagcgat gataaaccag atcattgatg
      721 gaccattaga ataaaaaaga gagaattgga tgaccaagcc agctaatgca taagtgatca
      781 ctgccagttt gtacataatc atatggagtg ctgtcacccc tgctccctgg gatagcttca
      841 ttcctttttt gttataaaaa taaatctccg agatcagcgt tcctgtacca aacgaaacga
      901 ttcggtaaaa cgcaatatag ctagaggtca agaacccatc tttcgttgta aaattcggcg
      961 cgaaaggttc agcaatttct ttgattgagc gcccttcagc gatttggtaa actgtcccta
     1021 atataacagc cgctgccaaa acaacaacac tggtagacag caattcggca aagatatctg
     1081 caaatgattg attgattaaa taataaataa tggcaaaaat cacaatcagc aaaagcagat
     1141 tcagaatgat tttagttttc gttcctttag acgacatctc ctgctcctta atggaaaaaa
     1201 taaatgagaa tcaaatcaac aagtacacct gcagcgaatc ctatagtaaa cttctttttc
     1261 cgagttttat gatggaaaac ttttcgagag atcaatcctc ccaaaccacc gcctaacagg
     1321 ctgacaaata tcaaattatt ctcaggaaca cgccagccgc tagttctagc ctgatgttta
     1381 tcatacccca tcagacaaaa taagtaaaaa ttcacaatga aaaggtaaaa ccacaaagga
     1441 ttgtgtaaca atacatccat tactcttctt ccaactttct gttaactact atttctaagc
     1501 tttttcttct ttctttggtg aataaactgt taatcctttt gatacaacag ataattcaac
     1561 tggaagatca tctgtcgtgt ctccatccgt tcgagtgccc acagattttt cctgcgcttg
     1621 gattttcatg cgtttggctg tgaaataagt aatgccgggg atcgtataga atttgcctct
     1681 catgattcta ggcaaataaa aaataaatcg gaaaatatct aaggaaggta cgatcgttac
     1741 atgaaatttt ccatcatcta gggtcgctga gtcgtcaaaa tttgtaaaac cgcccacgga
     1801 gttagacatc gtgactgtca acagttggga ttttcctttc caatgctcct cgtctgtttc
     1861 taaatccaaa tgatattttt tcttcttcag caataactta aaaaacttct tgataaagat
     1921 ccacgagccg taacgctgtt tttcttcttg actgatccaa actgctgtat ctgctaagat
     1981 tccgattgtc agtgtagaga taatgacttc atcattgact tgaccatagt ctattttccg
     2041 tacattgcct tccaggatca cgtcagcagc ttcttctttt ttgaggggaa tctcaagaac
     2101 acgtgcaagg ttgttgacag ttccacctgg aagtaatccg acatggtatt gagataatgt
     2161 tttttggaac gcttggacga tatgatgaat cgtgccgtct ccacctataa ttactaaagt
     2221 atcagcttct atggcttctg ctttcttgac taattccgca tcatcaatcg atgggccagt
     2281 ttcagctagt tctacatata attctggctg cttttctttt acataatctt gaaaccagcg
     2341 tgctaatttc tcaccctcat ctttccctga acttttgtta taaaaaacta gcagcttttc
     2401 cattctttac ctcctattta ctcttcaaaa aagagggcgg gacaaccaat tccattgtcc
     2461 tgccctttag ccttagacag atgacattcc atcattggga cgaacgcctt gtcataagct
     2521 gttctttttc gattttaact ctttgtgttc aattctgcaa tttttacaat cacgtctgtt
     2581 gctttttcca ttacttgtaa agaaacaaat tcgaatcgtc cgtgcatatt ttctcctccg
     2641 gcaaatagat ttggcgtcgg aatacccatg taagagattt ttgaaccatc tgttcctcca
     2701 cgaactggtt cgatcactgg agagatacca agctcgatca ttgcttgttt cgctaattcc
     2761 acgatgctca tatctttttc aatgatttct ttcatgttgt aatattgatc atacatatct
     2821 actttgattc gttcttgatc aaatcgctga ttcaactctt cttggatatg tgtgatttgt
     2881 gctttgcgtg cttcgaattt ttcacgatca tgatcgcgaa tgatataact catagaagct
     2941 tcttcaggat tcccatccaa tgccatcaaa tggaagaaac cttcgtatcc ttccgttttt
     3001 tctggaactt catcggctgg aagctgatta tgaaaatcga ttgctaactg tagagcattg
     3061 atcattgtat cttttgccgt ccctggatgt acatttttcc cttggatcgt aatatttgct
     3121 tgagcagcac taaacgtttc atactgcaac tcacctaaag gaccaccatc gactgtataa
     3181 gcaaaatcta catcgaattg ctcaacatcg aatttatccg ctccgacacc gatttcttca
     3241 tcaggaccaa aagccacttt gatggtaccg tgagggatag aaggattatt gatcaagatc
     3301 tccattgcag tcatgatttc cgcaatccct gatttgtcat ctgctcccag taatgtactg
     3361 ccatctgttg tgatcaatgt gtggccttca taatttttca aatttggaaa atcttttgta
     3421 ttcaatgtat agcgttcttc cgcatctagt acgatcgtag actcgccatc atagttttca
     3481 atgatttgtg gattgactcc tactgcatta aaatctgctg tatccatgtg ggaaataaat
     3541 ccaatggaac gcacttcatg atcaacattg cttggtaatg ttcccataac gaatccgtta
     3601 tcttcattat agaaaacatc agctaagcct aactcttcta attctttttt caattcttgc
     3661 gcaaaagcaa cttgtgtctg agtcgatgga gtcgttttgc ttgttgcatc ggagcgtgtt
     3721 tctgttttga catatcgtaa aaaacgtggc aaaagatttt cgtacatagt attctctcct
     3781 tttaattaaa ttgaaatggg tttgtatttg tttcagaaac gaaaaagtta atatcccaat
     3841 tttcttcttg tttccaagat tcaaatttct cgataaatcg ttgcttgcat aacgattcaa
     3901 tataatgacc gggatcaatt gcaatcaacc cagcgctttg catatcatga gccgtatgat
     3961 aatagatatc tcccgtgata tagacatctg ctctttgggc gattgcttgc ggataaaatt
     4021 tttcaccgct tccaccgcaa atcgcaatgc gcttcacaga ggattttgca ttttttggct
     4081 ggactattct cagtccatct agttggaacg cttcttttac ttgttctaca aatgcctcta
     4141 tagaaatttc ttgcggcaat tcacctactc gtcctaaccc aaacatttcg actggttcat
     4201 ctatagcgaa aagatcataa gcaggttcct cataaggatg agctgaccgc atggcttgaa
     4261 tcacttgttt ttcgatagtt tccggcaaaa taacctcaac ttttgcctct tgaacttgtt
     4321 ctgtcttccc tactttccca atcgctggtt gtgcaccagc ttccggagta aatcgaccgt
     4381 gtccaatcga ggtaaaactt gtaccggtat aatcaccttg tgtaccagca ccagctgcag
     4441 ctaatacttc acgcattttc tgtgcatgat ccacaggtac ataaacggcc agttttttat
     4501 aatgaatctc atgagtcttt actaaataac tctccacttc aataccgaga agttcacaaa
     4561 accagtcatt cagtccatcc caaataatat ccatgttcgt gtgtgctgca tagacggcaa
     4621 tatcatgttt caacaaatct gcatacattt tttcttgtgg ctgatcagta accagccgtt
     4681 ttacaggacg aaagattgga ggatgtttag caatcagcaa atcgattttt ttcttgattg
     4741 cttcttcgac cacttctgga cgaacgtcta atgtcatcat tactcgttgg attggcttat
     4801 ccaatgttcc gatatgcagt ccgactgggt ctccttcttc agctagccac tgaggacaat
     4861 agctttcaaa cttttggatg aaagtgcgtc cgctgattgt catggcttta aaacctcctc
     4921 aatcatttgg atttcctgct cgatttgttt ttgacgtgcc tcttgattag ctgcttgttt
     4981 gagctgttca cggatagctt ttctttgttg aagctctctt gtccattttg atttgaagat
     5041 ctcacttttc tcttcaagca gaaatggccc gaacatcagc tccatttcat tgtaagtaac
     5101 tggtttttgt tctttttcgg ctacaatgat ttcatatatt tttttatttt cttcaagtat
     5161 tttttctgat tggattttat actgattttc ttgcagccaa gatcgtaatg tacgctctcc
     5221 gatgtttggc tgaaggatca aacgttcttg tccgcttaag cgttgattca acttgccagc
     5281 ttctaggata tctcggatca gtgaacctcc catacccgca atcgtgacag ctgtaatacc
     5341 atcttgtggc tggatagcat ctagaccatt tgcaaggcgt acggtgatac ggtcttctaa
     5401 tccatctttt ttgacttgct tacgtgctga ttcataagga ccttgaacga cttcaccagc
     5461 aactgcatat tcgatttttc ctcttaatat caatgcaacg ggtaagtaag cgtgatctga
     5521 tccaatgtct gctaaacgag cggattcagg gacaaagtca cccactgcgg ctaagcgctg
     5581 agataaatct aaatggttca tagcttcact ccttctagat aattatagca ttttccaaat
     5641 aaaataagga gactaggaat aaaaaaattc ccatatctcc ttaaaaaata taccttttcg
     5701 ttctataaaa
//
LOCUS       DAZULY010000167         5684 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000167 DAZULY010000000
VERSION     DAZULY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.168"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(169..951)
                     /locus_tag="ACG81A_002158"
     CDS             complement(169..951)
                     /locus_tag="ACG81A_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015486.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1016..1852)
                     /locus_tag="ACG81A_002159"
     CDS             complement(1016..1852)
                     /locus_tag="ACG81A_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5015487.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1870..3279)
                     /locus_tag="ACG81A_002160"
     CDS             complement(1870..3279)
                     /locus_tag="ACG81A_002160"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5015488.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3293..5116)
                     /locus_tag="ACG81A_002161"
     CDS             complement(3293..5116)
                     /locus_tag="ACG81A_002161"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFS5015489.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1730 a         1242 c          941 g         1771 t
ORIGIN      
        1 acgtttaatg aaactatctc cgtttcattt cagatacaaa taaatatagt cgggctccaa
       61 atccgaaaaa aaataccccc agtagcttat cctaagctac tggagatgta taagtacggt
      121 catataacaa cggggtaggg taaataaatg aaaaagatgg aagaatcatt attcaataac
      181 ttttttaaat acttcgttgt aatcagttac ctgtccgttc ttaaatacca cagaataatc
      241 ttttttccca ttttcaagta accgaagtgg aatgtcttta ccaacttgct ctgtcactgc
      301 attactgata cctgttagat tagctaataa atctgataaa gattgagcat ctgtagaaat
      361 ctctgtatca gtatttatcg tgattccttt atcttcattt acaagcttcc aagtaggtgg
      421 tatagctgtg tatgtttcga tgaatgaatc aattctttca tctatcgtct tcccagtatc
      481 tacttctgtc tcattactct tagatgaatc gattctttca tccatcgtct gctccgtaac
      541 tgcttctgtc tcattattct caccggaaac tctcttcaca cccaaactta tatattgtcc
      601 atctttagtg tgcatagtta aaattcccac gcttttacta ataaattcac tataaccagt
      661 tctttcaata gagacttcaa tgtatttcac actatcatac tcaaaagaaa aatagacatg
      721 taaaagcatt ttgttgttta catctacata ataatccttt gactctactt gagaccttcc
      781 tatacggaat acagctttac tgtctgatat ctctatacca gcatccttat tattaatctg
      841 ccaatctcca agtagctctt ctggcaagct agtagtttct tgtgaacact ttaccacttc
      901 actggtagag gtcgaaacat tcacctccgc actgttagaa gaattttcca aggaatcagg
      961 agccttagca caaccaacaa gtctttatat attgtgcagc cgatattttc ttcattcagt
     1021 acacccttga aatatgcagt gttagataaa gcagttcgtc ctgatttgct gaggcaccca
     1081 tctcttcttg aacgtactcc ttgattttca aggccgcccg atactctttc ggaaaacgct
     1141 gcttcactga agccagcaaa aaatcatcct tctccggcaa ctcttccttc ttaatcattc
     1201 gggcaataaa aaactgtaaa tgcgtcacaa atcttaagta aagcatcgaa tctttatcaa
     1261 cctttttttc gggaaacaga tatcctagca tagccaagat atcctttagt gctcttaccg
     1321 agagcatggt ttcttccata tttgcttgtt cactttgggc atttacaatg tgaaacgcaa
     1381 tgtttccagc ctcctcttct ggcaactgaa cattcatttt tttgttgatc aattggatag
     1441 catagcagcc gatctcaaat tcttttgggt aatacttttg aatttcaatc agcaggcgat
     1501 tttgaaaaac aatgcctttt tgactgcgtt caatggcatg cttcaagtga tcaaccaagg
     1561 taaaaaatag acttttattc agcttaaagt caaacttggc gttggcataa tcgataattc
     1621 gcttgacgca aataattaga tcatccggag cagcctcaaa cagtttaatg taatcctttt
     1681 ttagagcatc agaaagtgaa tctaatttaa atactttttg gattttgtca gccggaacca
     1741 aatcgccttt ctttagttga aatccaatcc ctttccccat caaaattact tctttgcctt
     1801 gcttatctaa gcaaagaatt aaactgttgt ttaacgtttt aattactctc attagattca
     1861 ccgttctctc taagctaagc catttcttgc gattactttt tgataccaaa agaagctttc
     1921 tttttttatt cgttttaatt ctttaagatt ctcatcggtt cggtcaacat atataaaacc
     1981 ataacgctta ttgaagcctt gatgggtact aacaacatca atcgctgacc aaggtaaata
     2041 accaaaaaca tctacaccat ctgttaaagc tgcttgaata gccagaatat gtttggctag
     2101 ataatcaatt cgatcctggt cattaattcg tctatcttcc tcaactatgt cgggtgcccc
     2161 atagccattt tcagtaatta aaataggtaa atcatagcga tcgtacaatt tcctcaatgt
     2221 taatcgtagt ccagtagggt caactaccca gccatatggc gttttatcta cataaggatt
     2281 ttcagctgcc cggtagacac cttcttctcc caacatgatc tgttgatccc ccgctctagc
     2341 ggaaacatct gatccgtcat ttttgctggc agcgatggtg gcagttgaat aataattaat
     2401 cgcaataaaa tcgggtttac cggaggataa aattgcctga tcctctgcct caattttagg
     2461 atataatcca cgatcattca aatagttttc aaacaaataa ttgtattttc cccgaactgc
     2521 tacatctaag aaagaccagc ctctcaatgt ttcccaatta tgggctgcaa ttgcatcttc
     2581 tggactggag gtagcttgat acatagccgt taaattgatt gctggaccga tttttccttt
     2641 aagattcaac tgatgataca agtgaattac tctagcttga gctaataaaa catggtgatt
     2701 aatttgaaag agttcttttt ttgaaggcag ctctccactt ggtggcaaac caatcgctcc
     2761 aggatgaagg atcatcgtgt tttgctcatt aattgtcagc caatagttca ccttgtcacc
     2821 atactcagta agcaatacct gtacatagtc cacaaaataa tcgatagttg ctcgatttaa
     2881 ccaaccgccc tgctcctcta gaaaataagg taaatcgaaa tggtacatgg tgacaatcgg
     2941 ttcgattccc tgattcaggc attcatcaat tacttgatga taaaaagaaa ttcccttttg
     3001 attggtttta ttattttcaa tatccataat tcgcgtccaa gcgatggaaa agcgaaaagc
     3061 ttttaagcct aattctttaa acagctgtat atcttcttta tagtgatggt aaaaatccga
     3121 ggcaacttca aaattagcta ccccttctgt atgctgatac ttgtcaataa tagacggttg
     3181 tttcccatcc tcctgatagg caccctctgc ctggtaggca ctaatggagg caccccataa
     3241 aaacttctct ggaaatgctg aaagctgttt gtattgcaca aaaatcctct ccttaactaa
     3301 tttgcatcaa tggttcgtgt tcctttaagc ttccgcttgc ttcactaatt cctgcgtaat
     3361 tttcactgtt ggtcactacc acaatcactg tatcatcgta gccagcgttt ctaatggcag
     3421 cgaaatcggc gttgattaat aattgtcctg ctgacacctg atctcctgct tttacaaata
     3481 cttcaaaggg ttcccctttt aaattcacgg tgtcgatccc cacatggatc aataattcaa
     3541 taccttcttt agaacgtaga ccaatcgcat gtttagattc taaaatagta atgacttctc
     3601 cctcaacagg tgaataaatg ctgttttctt ctggatgaat ggctacgccc tttcccaata
     3661 gtcccttaga aaagacagca tcgttcactt cagaaatagg acacacggtt ccttttaccg
     3721 gattttcaac aatgactgct tcggctattg ccgtttgctc tgattcatca gaagtatcgt
     3781 caaatccaat tagataagca atgataaagg ttgcaacaaa ggcgattaca cacgtaataa
     3841 ttgcgtgaac aatattcatt ggattttctc caataaaggc tggtaaggct gctaagccag
     3901 gggaaacaaa ggcatatctg actaaaccgc tcaagccagc atacaaacca gcaattgcac
     3961 caccaatcat ggaagcaact aatggttttt tcagcttcaa ggttactcca tagagtgaag
     4021 gctcagtaat tcccattaaa gcagtgatcc cagtggacac agctagctgc ttcaactctt
     4081 tgtttttagt tttcgcggct acagcaaagg tagcacctga ttgagcaata ttagaagcca
     4141 gcattcctgg cccattgatc atttcagaac ccgtactgct caattgcata gtagcgattg
     4201 gcgtcattgc ccaagctgta cccgtaacca ccaaaaatgg ctgcaaacca cccatcaaaa
     4261 atggaattaa ccaagctgct ttgccgttaa tccattcagc ccccattgct ataagatcgt
     4321 tcagcaacgt tccaaacggt ccaatgacaa ccaacgctaa aggccccata attaaaaata
     4381 ccagcatcgg cttagtaaag aatttaataa acgaaggcga aattcgatcc gcaaagggct
     4441 ctacatagga catgatccaa acagtaaaga taatcggtaa aactgatccc gcataatctg
     4501 cagctaatac cggaattcct agaaaagaaa cggcatcgcc agtagaaaaa agctgtgaaa
     4561 tatttggatg aagcaaaatg ccagcaacag taatcgccaa aattgggttg acatcaaact
     4621 ttatggctgc cccataggct aatagtactg gcataaaata aaaggctgca tcagcaatag
     4681 tattcaaaat gatgtaagtt tgactactgt ctgatagtac accagtcaaa gtcaagacgg
     4741 ccaaaatggc ttttaccatc ccggcacctg ttatagctgg aatcatcggt gtaaaggtgg
     4801 tagaaatgac actaatcaat cttgaaataa tactttcttg attgtctgaa gaggggccct
     4861 ttttaattga agttcccact tccttttgaa tttccttgaa ggctgtttgg acttcattgc
     4921 caatcaccac ttggtattgt ccaccgctat ccaccacact aattacacca agtgtttttt
     4981 ctaaggcttt cgtatcggct ttccttttat ctttgagttc aaaacgcagt ctggtggcac
     5041 aatgggttaa gctgaccaca ttttcttttc ccccaacctg ttgcaataca tttcttccta
     5101 cttctctgta atccattatt ctctctcctt aaagcagtaa aaaagaccaa gtcaaaccac
     5161 gccccaatca acattgaagt gttagtttgt cttggtcttg cctgcataat cagtaacatg
     5221 ccaataacgt tattttgctg taatccaata atagcacatt atgatagcgc aatcaacagg
     5281 gaatttaatt ttttatcttt tcgtttgacc gtatagggta atataacagt tagatcacat
     5341 caactagggt atatctgcgt atacttactg ctaaaataag tatatctaat attaactata
     5401 ccagtaaaac cattcctaca taaccagcgg gaaattccca aaatcgtgaa agcatcaaaa
     5461 atgcatataa cggatcacac aacttcaaca attctgctct tgggaaaaat tgatgaattt
     5521 ggtgagtaaa ggatggaata aacagcattc caacaatata ggcaacaata gtgatacttg
     5581 tttttttggt aaaaatcagt aagcaatagc caatttcaat cattccgaga tataaaggta
     5641 tcgatagaat gatttgggct agtattctta aaaattaact agca
//
LOCUS       DAZULY010000168         5650 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000168 DAZULY010000000
VERSION     DAZULY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.169"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            163..1155
                     /locus_tag="ACG81A_002162"
     CDS             163..1155
                     /locus_tag="ACG81A_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5015490.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1215..2636
                     /locus_tag="ACG81A_002163"
     CDS             1215..2636
                     /locus_tag="ACG81A_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFS5015491.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            2629..4128
                     /locus_tag="ACG81A_002164"
     CDS             2629..4128
                     /locus_tag="ACG81A_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFS5015492.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4167..5561
                     /gene="uxaC"
                     /locus_tag="ACG81A_002165"
     CDS             4167..5561
                     /gene="uxaC"
                     /locus_tag="ACG81A_002165"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS5015493.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         1853 a         1048 c         1163 g         1586 t
ORIGIN      
        1 atgagcattc cccgctttca agaatagcat aattaaaaat aaaaatccaa gtaattttta
       61 acgtgtgcaa tcttttcata tcaaagatct attgacttat cgtagttatc gtaatatgat
      121 gaatgcgtta acaaaaagtg aaaaagtaag gaaaatgatg gaatggtaac gatcaaagat
      181 atcgctaaag cagctggcgt ttctcatacg acagtttctc gtgctttgaa taacagcagt
      241 ttaataaaaa atgaaacaaa acaaaaaata cgacaactag ctaaggaaat gaattattcg
      301 ccaaactata gtgcaaaagg tttagtgaat caaaaaaaat atctaatcgg actatttttc
      361 tcaagtttgc ataaaggaac ttcatcaaat ttcctagcag attccattgc aggcatccat
      421 tcagtattag acacaaatta cagtttatct gttgaaagta tcgatgttgt tgatttaaca
      481 gaaatcaacc tgcaacgcta cgatggactg atcgttatga gtcaaagtga tgatgatcag
      541 cctttcatcg actatctcaa agaaaaagaa tttcctttcg ttgttgtcaa tcgtcacatc
      601 aatgatccag cggttatcaa cgtcactgcc gatgatgcgg ccggtgttat gcaggcagta
      661 gattacggta ttggatgcgg acatacgaag atcgcttata ttggcggaaa gaaaaatttt
      721 cgttcgacaa aagaacggga aaaaggggta ttgaaaagtc taaaaaaagc gagcatccct
      781 gtcaatctag cctatttttt aacaggagat tacagcttag aaagcggcct ccaaaacatg
      841 gaatacctat tatcactttc tgacctgcct actctggttt tttgcggcaa cgacgacata
      901 gcgattggtg cattgagagc tgtacattta gctggcctcc gtgttcccca gaacatttct
      961 ctgatcgggt tcgatgattc tccagtcgtc tcctatctca atccgccgct taccaccgta
     1021 cgtaagcctt tgaaagaaat gtgtcaagaa ggaacaaagc ttttactaga tttgatcgaa
     1081 ggcaggacga tcgaacaatc taaaattgag ctctccacct tgttgatcct tcgtgagtcg
     1141 atcaattact tgtaaactac aagtaatttt atataaaaat gttaacgtgt gcataacaca
     1201 gaaaggaagt ttatatgaaa aaactgacaa aagaattagt gaaaaaaaac gaagcacctg
     1261 taaaagttct tcagtttggt gaaggcaatt ttatgcgtgg atttattgat tggcagattc
     1321 agcagctgaa taaccaacaa ttgtttaaag gtaatgttgc aatcattcaa cctttagaac
     1381 aaggtcttgg aaatatgatg aaccagcaag ataatttata tacggtcatt ttacaaggac
     1441 tcagcaatgg cgaaatcgtt gatacttccg agattatcac cgtagtagaa caaatgatca
     1501 atccttatga tgaatgggat gcttatctat ctctagcaga aaatgacgac ttggcctata
     1561 tcgtctccaa tacaacagag gcaggaatcc aattcgtgga gactgataat ttaacagata
     1621 ctcccccaca gagcttcccg ggcaaactga cagccttttt ataccgacgt ttcaaattaa
     1681 acaaagctgg ctttaccatc attccttgcg aactgattga cagaaacggc gaacgtctaa
     1741 aagaaatcat tttacgctac gcagaaaaat gggatttaga acctgctttt gttgagtggt
     1801 tagaaaaaga aaatgttttc tgttgtagtt tagttgatcg catcgtccca ggttatcctc
     1861 gcgaagatgc aggtgttttc aatcaacggc ataactatga agatcaattg atggtcaaag
     1921 cagaaccatt catgctttgg gtgatcgaag ggcctaaaga attggagaat caactaccat
     1981 tcaaacaggc tggcttgaat gtcatcatca cagatgatat gactccttat cgtgaacgta
     2041 aggtgcattt gcttaatgga ccgcatacag cgatggtccc tcttgctttg ttggcggaat
     2101 tggaaactgt agaagaagta atgaaagatc ctttattctc cgcatatgtt gatcagttat
     2161 tcaatctaga attgattcct atgctttctt tacctaaaga tgaattagcc atttatgctg
     2221 accaaatcaa agaacgtttc ttgaatcctt ttgcccatca caaattagaa gcgatttcat
     2281 taaatagtgt ttcaaagttc agcacccgtc tgcttccagt gttcaagaag tatatcgaag
     2341 aacaaaatca ggtaccaccg ttaatcactg tttcactagc tgcgctactt cttatgtatc
     2401 gaggcgatca agtaaagcca catgatgatg aaaagacgat aagcgaattt actgatgctt
     2461 ggtctgataa tggaacagct attcctcgat tattgcaaaa cgctgcgctt tggggagaag
     2521 atttgtctca aataccgaat gtgacagata cagttcaaga aatagctgca aagatcgatc
     2581 aaaaaggtgt ccggtcagta ttacaagaaa tgaaaggaac gatggtccat gcttgaaacc
     2641 ctgcaaccaa caatgaaatt aaatcgacga gacagtgtgg tcgtcgcatt aacccctatt
     2701 ccaaaacaaa cacaactgac gattgatcag caaacgatca ccactcttga agatatcccg
     2761 caaggtcata agattgcatt gggtgatttg aaagctggag acaacgtgat caaatatggc
     2821 tatccaatcg gtcatgtgac gacagacgta actgccggtc aatggctgca cactcataat
     2881 gtaaaaacca atctttctgg tgaattggat tatcgatatg aaaaagatgt ccatccaagt
     2941 cattaccttt ttgagaaccg aacatttcaa ggttacttgc gaaaaaatgg aaaagtaggg
     3001 attcgtaatg atttgttttt agtacctacc gttgggtgtg tgaatggcat tgctgaactg
     3061 atcgtcaaac agttcaagga aaaacatcca gatctgggac agtttgacca tattacgatt
     3121 ttaaagcatc cttatggctg ttcgcagctt ggaaaagatc atcaaaatac tcgagaaatt
     3181 ttagcggacg cagtaaatca tcccaatgcc ggaggagttt tagtatttgg attaggttgt
     3241 gagaacaata cagttcctga attcaaaaaa attttaggtg aatatgatga agaacgagtg
     3301 aaatttctag tggctcaaga tgtttacgat gagatcgaac aaggtgtggc tctattagaa
     3361 gagcttttaa cagcggcgga aaatgaccag cgcatatcag tcccgctctc taagttgaat
     3421 gtcggtttga aatgcggcgg ttctgatggt ctatcgggaa taactgctaa tcctttactt
     3481 ggtgcatttt ctgactactt gattgctcaa ggaggcagca cgatcttaac agaagtgcca
     3541 gaaatgtttg gcgcagaaca agtattgatg gcacgagcgg aaaatgaaga agtttttgac
     3601 tcaatcgttg acttgatcaa tgacttcaaa cagtatttct tgtcttacgg ggaaccagtt
     3661 tacgaaaacc catctcctgg taataaagca ggtgggatca ctacattaga agataagtct
     3721 ttaggctgca cgcaaaaatc aggaagctct cctgtcgttg atgtgctaca atatggcgaa
     3781 aaaatcagaa aaccagggct tagtttattg caagctcccg ggaatgatct tgtagctgcc
     3841 tctgctcttg cttcatccga ttgccagttg gtcttgttta ccacaggaag agggactccg
     3901 tttggctcat atgtcccaac ggtcaaggta tctacgaata caactctttt tgaacgcaaa
     3961 ggtcattgga tggattttaa tgcaggcgtc ttattagaac aaccaatgga agtaatctta
     4021 gaatcgtttg ttcaaaaaat catcgctgta gctagtggag aagaaacgaa aaatgaacaa
     4081 aatgacgttc gagaaattgc catttttaaa aacggagtaa cactttgatt ttcataaaac
     4141 agtataaaaa gaaagaagga agatttatgt ttttatctga tgatttttta ttaaaagacg
     4201 attgggcgaa aaagctttac cattcctacg caaaaaacat gcccattatt gattatcact
     4261 gtcacttaga tccaaaagaa atttacgaaa acaacaattt cagcaatctg acagaagctt
     4321 ggttaggagg cgatcattac aaatggcgct tgatgcgagc ttgcggcgtg cctgaagaaa
     4381 aaatcacagg aaatgcttct gattttgaaa aatttttagc ttggtgtcaa accgtaccaa
     4441 aaattatcgg aaatccgctt tacagttggt cacatcttga attgaagcgt ttcttcggta
     4501 ttgacttgtt gataaatgaa gaaaatgcag agaaaatttg gacacaagca aatgaaattt
     4561 tggcagctcc tgattttcgc cgtcgggaaa tcgcaaaaaa atcgaatgta gaagtgattt
     4621 gtacaacaga tgatcccgca gacgacttgc actatcatca gttgcttgct catgaagaac
     4681 cagatttgaa ggttctacct tcattccgtc cagataaagc actcaatatt gaaaaagaag
     4741 gatttaatgc atgggtcaca ctactggaag aagcagctga ccaaacgatc accacctatc
     4801 aagaactgat agaagtctta gggcagcgga tagaatactt ccatcaacat ggatgtcgtg
     4861 tttccgacca cgctttagac atcttacgct ataaagaagc ggatgaaaca gtattagaag
     4921 agatttttca aaaagcacga gctaacaaac tattatcttc agatgaaatc gatgcttatc
     4981 ttacagaaac actgatacat ttgacccact tctatcattc tcataattgg acgatgcagc
     5041 tgcacatcca tgcttatcgt aactgtaata cacaaatgtt ggaaaaatta gggcccgata
     5101 ccggctatga tggaatgaat gatctttctt tgactattcc tctgcaaaaa ttattgaacc
     5161 gtgcagaaga aacggatcag ttgccaaaga cgatccttta ttcattgaat ccgaatgact
     5221 atcctgcgat tttagctttg atgggctgtt tccaaaaaga acagaatgga aaactgcagt
     5281 taggttctgg atggtggtac aacgatactc gagctggtat gcgcgatcaa ttgacacaat
     5341 ttgctgatgg tagtgctttg gggaattttg tcggcatgct gacagattca agaagtttcc
     5401 tgtcctacac tagacatgaa tatttccgtc gtgttctttg tgaattttta ggcgaaatgg
     5461 tcatgcgagg agaagcacct gaagatgaac aacttttagg aagtctagtt caagcgatta
     5521 gttatacaaa tgctaaaaat tactttggct tttttgccta attgattaat agtaacagta
     5581 acgaatcaaa aacaggatag tgaaataacg gaatccgttt atccacgttt tagagaagaa
     5641 gacggaccaa
//
LOCUS       DAZULY010000169         5627 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000169 DAZULY010000000
VERSION     DAZULY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.170"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            164..2698
                     /gene="secA"
                     /locus_tag="ACG81A_002166"
     CDS             164..2698
                     /gene="secA"
                     /locus_tag="ACG81A_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFS5015494.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2824..3925
                     /gene="prfB"
                     /locus_tag="ACG81A_002167"
     CDS             join(2824..2895,2897..3925)
                     /gene="prfB"
                     /locus_tag="ACG81A_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFS5015495.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4030..4716
                     /gene="ftsE"
                     /locus_tag="ACG81A_002168"
     CDS             4030..4716
                     /gene="ftsE"
                     /locus_tag="ACG81A_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFS5015496.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4709..5593
                     /gene="ftsX"
                     /locus_tag="ACG81A_002169"
     CDS             4709..5593
                     /gene="ftsX"
                     /locus_tag="ACG81A_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFS5015497.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
BASE COUNT         1930 a          989 c         1259 g         1449 t
ORIGIN      
        1 ttttttgagt ttcctcctct tttagatgaa atatgaataa attatgaagt tcttacttta
       61 atacgcttaa gatatttcaa ttatctataa ctaatgataa aatgaaaagt aaagtctaca
      121 tagtctggat aatacacaga aggaaaagga gattacacaa cgcatggcca atttgattcg
      181 ttcacttatc gaaaacgata agaaagaact aaaacgttta gatagcattg caaaaaaagt
      241 tgaagcatac gctgaccaaa tggcagcatt aactgatgaa caattacagg caaaaactgc
      301 tgaattcaag ggcagatacc aaaaaggaga aacattagat caattacttc cggaagcttt
      361 tgcagtcgtc cgagaagcag caaaacgtgt attggggtta tatccacacc acgtacaatt
      421 gatgggtgga atcgtgcttc acgatggaaa tatccctgaa atgcgtactg gtgaaggtaa
      481 aaccttgacc gcaacgatgc cagtatatct aaatgctctg actggcgaag gggtacacgt
      541 agttaccgtc aatgaatatt tagcaacccg tgactcgacg gaaatgggcg aactttataa
      601 tttcttagga ttaacagtgg ggttgaatat caactcaaaa tcttcggatg aaaaacgcga
      661 ggcttataat tgtgatatca cttatagtac gaataatgaa ttaggatttg actacttgag
      721 agacaatatg gtcgtttacc gccatcaaat ggtacaacgt ccgctgaatt atgccattgt
      781 cgatgaagtt gactcaatct tgattgatga agcgagaacg ccgctgatca tttcagggca
      841 agcagaaaaa tccacagctt tatatacaag agcagacaac ttcgtgaaac gtttgaaaga
      901 agaagaagat tataaaatcg atgtacaatc aaaaacaatc agtttaaccg aagcagggat
      961 cgaaaaagca gaagaaacat tcggtttgga taacttatac gacattgaaa atactgcatt
     1021 gacccatcac ttggatcaag cattgagagc caactatatc atgcttttag atatcgatta
     1081 tgttgtccaa gacggaaaag tactgattgt tgaccagttc actggtcgga tcatggatgg
     1141 acgccgttat tcagatggac ttcaccaagc aatcgaagct aaagaaggcg tagagatcga
     1201 agacgaaacg aaaaccatgg cgacgatcac tttccagaac tatttccgta tgtacaagaa
     1261 acttgcggga atgactggta cagctaaaac agaagaagaa gaattcagag aaatttataa
     1321 catccaagta taccagattc cgacaaatcg tccggttatc cgtgatgatc gtccagattt
     1381 gctttaccca acattacaaa gcaagttcca cgctgtcgtg caagatatca aagatcgcta
     1441 ccgcaaaggt caacctgtgt tagtcggaac tgttgcggtt gaaacatctg agttattatc
     1501 agatatgctg aatcaagaac gtattcctca tgaagtgctg aatgcgaaaa atcactttaa
     1561 agaagcagag atcatcatga atgctggtca aaaaggtgct gtgacgattg ctaccaacat
     1621 ggctggtcgg ggaacagata tcaagttagg acttggggta agagaacttg gtggtctagc
     1681 agttatcggg acagaacgac atgaatctcg tcgtatcgat aaccagcttc gaggacgtgc
     1741 aggacgtcaa ggagaccctg gtatgtcaca gttctacttg tcattggaag atgatttgat
     1801 gaaacgattc ggttcagaac gaatcaaatc tttcttagat cggatgaaga tcggtgagga
     1861 agatgctgtt atccaaagca agatgctgac gaaacaagta gaatctgcac aaaaacgtgt
     1921 agaaggaaat aactatgaca cacgtaaaaa cgtcttacaa tacgatgatg tgatgcgtga
     1981 acaacgtgaa gtaatctata aacagcgaca agaagtaatc atggaagaaa aaagcttatc
     2041 caagccacta atgaacatgg tcaaacggac aatcagtcgt gtagtagatg cgcatacaca
     2101 attagagaaa gaaaattgga atttggaaag tatcatcgat tttgccggca gtactcttgt
     2161 ccatgaagat agtattactc ttgcagatat cgaaggaaaa acagctcagg agatcaaaga
     2221 gtatctaaac gaacgagcac aagaagtatt taataccaaa gcggctcaac taaatggtcc
     2281 agagcaactg ttggaatttg aaaaagtcgt gatcttgcgt gttgtggact ctaaatggac
     2341 agaccatatt gatgcaatgg atcaattacg tcagtcaatc ggcttacgtg cttatggaca
     2401 gaacaatcca ttagtcgaat atcaaacaga aggctataag atgtttgaag atatggtagg
     2461 cgccatcgag tatgaagtca ctcgaatctt tatgaaagct gagatccgtc aaaacgtaca
     2521 gcgtgaacag gtttctcaag gaacagcttc tcactcagaa gacggagatg ccaaagaaaa
     2581 tagtaatact agcgcgaaaa aacagccaat ccatgtgaac aaggtcggaa gaaatgatcc
     2641 ttgtccttgt ggaagcggga aaaaatataa gaactgtcat ggaaaaaatg caaactaatt
     2701 tctgacagca ggattggtga gacaaattct ttttttaaga attcgtctca ccttctttct
     2761 gttatttgct gattctgtta gactagatat aatgaaaaaa gtatagtgaa agaaggaaaa
     2821 aacatggaaa tatccgaaat tcgaaatctc ctgacagaaa tgcaggaaaa ggtcacaagc
     2881 ttcaggaggt ctctttgact tagaccagct ggaagaagac attgcggaag cagaagcaaa
     2941 aatggctgaa cctggttttt gggatgatag cgaagctgct caaaaagtga tcagtgaaaa
     3001 caatgcaaat aaagaaactt atgatcaatt ccattctctt gctgaagaac tggaagaaat
     3061 cgaattgatg gtggagatgt tgcaggaaga acctgatgca gccatgcaag aagaagcaga
     3121 aacacgtatc aatgcgctag atgaaaaaat gaagacatat gaattatcta tgttgttaga
     3181 tggtccttat gatcgaaaca acgccatcgt tgaattgcat ccaggggctg gcggaacgga
     3241 gtcccaagat tggggcagta tgcttttacg tatgtataca cgctgggcag agcagcatgg
     3301 gtatcaagta gagacactag attatcaagc aggtgatgaa gcaggaatca agagtgtgac
     3361 cctactgatc aaaggacaca acgcatttgg ttatctgaaa tcagaaaaag gtgttcatcg
     3421 ccttgtacgt atctcgcctt ttgactcagc gaaacgacgt catacttctt tttgttcggt
     3481 agatgtgatg ccagagcttg atgaaacgat cgatatagaa atcaatccag atgatttgaa
     3541 aatcgatacg taccgagcaa gtggtgcagg tggtcagcac atcaataaaa cagaatctgc
     3601 tgtacggatc actcacttgc ctacaggaac agtagtagct agtcaggcac aacgttcaca
     3661 attgaaaaac cgtgagcaag cgatggggat gttaaaagct aagctttatc aattagaagt
     3721 ggaaaagaaa gaacaagaag cagctgcact tcgaggagaa caaaaagaaa tcggctgggg
     3781 ttcacagatc cgttcttatg ttttccatcc atattctatg gtaaaagacc atcggacaaa
     3841 cgtagagaca ggaaatgtcc aagcagttat ggacggtgac cttgatttat ttattgacgg
     3901 ctatttaaaa atgcatctac aataagaacc cattgttaaa ttgaaataaa attgtaagat
     3961 tttgcaagca tgttgaaaca ttgccatgct ataatagcta aagactgaaa aagatatgga
     4021 gagaacgcca tgattgaaat gaaggatgta atgaagaagt actcaaatgg tacgaccgct
     4081 atccgcaatc tctcaattgt catcgaacaa ggagagtttg tctatgtggt tggtccgtct
     4141 ggagccggta aatctacatt catcaaattg atgtatcgag aagaaaaagc ttcaaaagga
     4201 actttgaacg tcgcaggtca tgatctaata gcaatcaaaa accgcgaagt tccttatcta
     4261 cgcagagaaa tcggcgtggt gttccaagac tataaattat taccaagaaa aacagtatat
     4321 gaaaatgttg cgtacgctat gcaagttatc ggcagaaaac ctcgggatat caaaaaacga
     4381 gtaatggaag tgctggacct tgttggtttg aaacacaaag ttcgagtctt tcctagcgaa
     4441 ttatccggtg gggaacagca acgtgtctct attgctcggg caatagtgaa tacgcctaaa
     4501 gtacttatcg cggatgaacc gacaggaaat ctggatccag aaaactcatg ggaaatcatg
     4561 aaattattag atcggatcaa tgcccaagga actactgtcg tgatggctac acataacagt
     4621 acgatcgtaa atacgattcg gcaccgagta attgctatcg aaaacggccg cattattcga
     4681 gatcaagcgg aaggggaata tggatacgat gattagaacc tttttccgcc atttattaga
     4741 aagtatcaag agtttgaaac gtaacggctg gatgacagtt gcttcagtca gtgcggtaac
     4801 gatcacattg actttagtcg gtgtattcat ggcagttatc atgaacgcaa ctaaattagc
     4861 tcaagatata gaaggaaacg ttgatgtctc tgtattcgta gatatcggta caaaagacga
     4921 agagatgaaa aagctagaaa aagaattgaa tgacttagat catgttaaaa aagttgaatt
     4981 ttcaagcaaa gaacaggaat tgaaaaaaat ccaaaacgaa atgggtgatt cctggaaatt
     5041 gtttgaaggt gacaataacc cgttatatga tgtgtatgta gtaagtgcta ctgatccttc
     5101 ttatacaaaa caagtagcag aagaagcagc agatttgaag aatgtttctc gttctgatta
     5161 cggtggagct tcctcagatc ggattttcca aatcgctggt gcagttcgga catggggatt
     5221 agttgcagcg gcattgctgc tgttcgtagc gatgtttctc atttcgaaca cgattcggat
     5281 cacgattttg tctagacagc gtgaaattca aatcatgcgt ttagtcggtg caaaaaatgg
     5341 atatattcgc tggccgtttt tccttgaagg tggctggatc ggtctgatcg gtgcgattct
     5401 tccgattttg atcatcacat ttggttatgc atggtttttc caattgatca atccatcatt
     5461 attacgttcc cattattcat tgattcatcc acaaaatatc gtttggaaaa tcaatctttt
     5521 aatggtcggc attggtgtga tcattggttc acttggctcg atcatttcta tgagacgttt
     5581 cttgaaattc taataccaca atataaataa aatgagaaag ttggaca
//
LOCUS       DAZULY010000170         5559 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000170 DAZULY010000000
VERSION     DAZULY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.172"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(60..2009)
                     /locus_tag="ACG81A_002170"
     CDS             complement(60..2009)
                     /locus_tag="ACG81A_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5015498.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2051..3109)
                     /locus_tag="ACG81A_002171"
     CDS             complement(2051..3109)
                     /locus_tag="ACG81A_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HFS5015499.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3228..3683)
                     /locus_tag="ACG81A_002172"
     CDS             complement(3228..3683)
                     /locus_tag="ACG81A_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015500.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4267..5070)
                     /locus_tag="ACG81A_002173"
     CDS             complement(4267..5070)
                     /locus_tag="ACG81A_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS5015501.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1631 a         1101 c          768 g         2059 t
ORIGIN      
        1 tcttacgacc catcgtttta ttctaaggtt ttgtagtaaa tatttcttga atttcatagc
       61 tacgcccctc caatcgaaaa tacaatcttg ttttctttac tttcagcaac ggaaggcttt
      121 gcaataattt cagcattaga atcacctttt ccttcaaacg ctccggcttc actcaactta
      181 acatcgaata tttttgcgtt tggataagta attattgctt cagtaatcgc ttcttcaaac
      241 tcagattcaa ctaaatgcaa gatattcctt gcaccccctg atttagaatc ctcttgtgaa
      301 gccatgtcaa aaactaagtg catggagaga tcgtctgcat aaaaattttg accatatact
      361 ctttctttcc taggtggaag acgtaaatct atatttaaat gttttcgtac taattcagca
      421 ctcttcaaca tttcattata agcaatttcc agaagagttt gttcggataa agcccaaaat
      481 ggaactattc gtccaaatcg gttaataact tcaggcttta gaccttcacc aatcaaggct
      541 tgtttgatcc ctgatgcgac ttttcggtat tctcttttta gttcagtgct gtattcatta
      601 gaattttcct tgtttgtaac acgagatagt gagctatatc ggtttaacga atcgaacatc
      661 tccgcacctg catttgttgt cgcaaataaa attgtatttg acaatgaaac ggtgtcgtga
      721 acttctcgat catcccatcc tctttcagta aaacttatcc taccttcatc taaaattgat
      781 aaaaaggcat cgagtgtttc tacaccagct ttctcatatt catcacacac aataatcgca
      841 gaaggatcat gccttacatg agtacctata agtcgtctaa aacgcattgc atcatctttg
      901 gttttgtagt ccggcatatt aactaagaga actttatttt catcgccata aagtcctctt
      961 gccacggctt tagccattgc agttttaccg acccctgttg atcctgtaaa cagagcagtt
     1021 gcttttggtt gctttcgacc tttaaaacga tcaaatccaa gcttcatacg tttaaacaac
     1081 attgttatag tataaactgc taacggttgt ccttttatgg actttaattc ctcttgaagt
     1141 ttttccatat caaaatcaga atctagctga atattatact taccaaatat ttgcaataga
     1201 ctttctcgat tataagcatt tccgtctact cgatcttttg caatcaaatc ctccattaca
     1261 gcaattgatt tagccggttc ggacaaatcc tcacgataaa gtttgtttgc ttgtacgatt
     1321 tcttgaagta attcatcagg aatcctttgc gataatctaa aagaagcctg aggatcatct
     1381 ttaacagagg atgtatttag ccattgtctc aaaatatcga cgacaacatc gctttttagt
     1441 tcattcaatt ctaacggttg tagccgtctc cctaaggcat catctctttt gatatagttt
     1501 tcatattctt ttcttgtagt agcggtaatc atttgaagtg attcatttgc taacggttct
     1561 tttagcgaat cacccccaat tttattgcca ctcccaaaag agctaacaac tttatgaaac
     1621 tcgtcaatga agaggacaaa ttcattatct ttgtcctcag cttttaaaat tcgctcatat
     1681 tctttcaatc ttggaattaa tgtttctaaa cgtgtttgga ggacatcatc tcccccaaca
     1741 cgcaattgtc cgattgataa tttatatact tgtactttct ttccatcttg tcgctgtctt
     1801 tcagcccatt cacttgcaag tgctgtttta cctgttccgg cttcacctaa caagatagct
     1861 tgcggtgttt ccggtctatt caagatcagt gacaactcat ggagttcgtc atcacgtccc
     1921 actaacttat gttttggtgg tttcattggt gtgagagcat gattaaattc ggtttgaata
     1981 ttcttaaaaa actcggtact attttccatc tgtttgtgtt cacttcctct aaaataagaa
     2041 taatttactt ctagtcagtc aaagttaggt acttaaatgt cgtcttcatg tctccaaaag
     2101 aatctttgtt aaagtcagca tttttgtatt gatcaatcaa tttttgtgta tcattgattg
     2161 ctttaatttc ggtctccatt cgactgtcta gttgacgaat actttcttgc gaatctttat
     2221 cgtcaggatt ttgttgtaaa cgttccagat actcagcttt tgtggcttca attttcttga
     2281 tgttagtatt tgaagcattt aactgatctt ctagcttatt tacaacatta ttcttacttg
     2341 taactgctac aacttttgaa taatcaatat cgccatcttt ggtaagaaag cttccttgat
     2401 agacattagt tgaatctgaa tatggattta ttctgaaatt gatattatca ggagtattaa
     2461 caggttttgc ttttttccca aaagaaatta ctccttcatt attaacatca ttcatagaat
     2521 attgagaaag tgcttttgta actgaatctg cttcattcac attgactttg gtatcatcgt
     2581 cagctgtatc atcaccagaa attagtgata cttggtttgc aataaaaact tgataagctc
     2641 tttcctctaa tttacctttt aggaataaga aagcatcccc atcagtactt aacatcccat
     2701 agctcatttc aagcttctta ggacgatctt tttcttttgt tgccatatat aagttatagt
     2761 tttttccttg cgtagataac aactttctag cgttttggga gtaaccaagt tttactactg
     2821 ttacatctct attttgatct gtccacacat ctgaaatagt gatttctgct cctgatcgag
     2881 caaactccaa tgaagaccct ataggtgttg tattgttcac gagataataa ttatagtttc
     2941 ctaattgata aatgtagcta cccaagcctg ttaataaaaa aagcactcct agaaaagttg
     3001 caagcataat gcgtaatccg tgacgtgttc tcttatcctt atcaccaagc aaccctccaa
     3061 taatactgta ttgatctttg gtagatgttg aattgacgtt tttttccatc agtttgtctg
     3121 ccccctttca taaaagagca aacaatcaga cgttttttcg gtaattctag gttcgggatt
     3181 ttgaatcatt tcggtatatt cttccccatt aatatttaac acaaaaatta tcttcctttc
     3241 ttcgttcctt tcttttgctc tttattttcc aaataatcaa tgatttttgc tattgtacca
     3301 agatagtaat tcccctcagc tttgtttttt cggattttgc taaccattgg agtaggtaca
     3361 atctcttcat ctctcaattc actgaaagat tttttaccta gatattttcc aataacagat
     3421 tttaaaaaac gttcaaaatc tttctcgtaa gttgttttct tgccttttag ttcatggttc
     3481 atattgtcaa cgacttttgc ggtttcaata attgttttag ttctcagctt aataatttca
     3541 tctggaattt tcaatcctcg taatttcatc gttgtactaa ctggtaaccc atgctttact
     3601 gcatcagcaa tactaaaact ttttagatca tctagtaagc tctccatata gttaacaaat
     3661 tcatgccaat taatagcatt cattgtctca ccccttcatt tttttaaaat gaggttcggg
     3721 cgtaacacat ttatgttatt ataatatcat aataacataa ataaatcaat cagcaaattt
     3781 agatatccct taaagtatca gcaattattc aacaagatat ttattttagc tatttcagtc
     3841 atcataaaag actaattagc aataaaataa ctaatattat ccatttatca gacgaaaaga
     3901 aaaaacttat ctaattactc tatattaact tttaacaaat agttgacttg tccaaaaatg
     3961 ttaccttcaa tctcacaaag atattaagat gtttcctacc aaaactgata tcaccggact
     4021 ttttattctg tgttgtccaa aagtgttacc tttagtcgat caacaaatta aaaaaaattg
     4081 tttttttctt gacttgtcca aaagtgttac ccatagttgt taaaatttgt atagattagc
     4141 cattctttcg aatattattt agactgaaac ttgcttacaa aaaaacttct tgcatttatt
     4201 gtccaaaagt gttacccata cttttttaaa gaagcaggtt gtattaaaat tacaacctgc
     4261 ttcttctcaa tcttcttcta accaattatg cattgaaatg gttggcaagt ttgattctgg
     4321 ctcactaaaa gtgaatctga gccgatcaac tcttcttcct ttctttgcat agattttttc
     4381 ataatcaaaa gtttcaaata cctgtattcc gttttcatca ggagctaaaa attcttcttt
     4441 cgcctgctct agtattcttt tgtcaatatt tgtcattcta taacttgaag gtatacttaa
     4501 tgctcttcta aaatcttcaa ctttgactac ccaataacca tttcgccact ctttatttct
     4561 gtattgcatg agatgacgat acaattcttt tgtgtagcta cttcttagtt ttgtaaaatt
     4621 atcaagttca aaacgagtaa agtttttgcc aattgagttt aagataaatt caaattcctc
     4681 atttgcctta attgtaactg tttccttctg tttatctatc cggaattttt gaaaaagcca
     4741 cagctgttca tagacgtctg aatcttcaaa acggtaggtt aaagtaccta gtttgtcact
     4801 caaagctttt aacacagaat aaaaactgtc cttttctctt ctgtcgtatt tgaccaaata
     4861 acgaaagagg ttgaaatcaa aagttacttc tgctgttcct tgacgtttca tttttgataa
     4921 aatagcccaa aacacatcca tttcaacagg cttaaatcct cgcaaagctg ttgtattcag
     4981 accattgtta taacgcacaa ttgcatttgt ttgttctgat actaaatcta catcactatc
     5041 aataatcttt tcggtaactt cttgagacat tgattactct ccaatcttct ttttgcgctc
     5101 ctttaatcat gttaatctgg tactaattaa aggaaacaat tttttctgct tgttacctaa
     5161 attaaatcac aactaaaggt aacagtcaac aaagatttgt tactaatcta attttctatt
     5221 ttttcgcctt ccaaatcaag aaaaccgaag gtaacgtctt atcctagtgt gatacctcaa
     5281 atgtccactt ttgttacctt tgctgtccaa aagtgttacc cttactgtcc acttttgtta
     5341 cctttactgt ccaaaagtgt tacctttata cccctttaat acttactctc ccaagagatt
     5401 aagcctcccc taacagatat attaacagat atttaacaga tatattaaca gatatttaat
     5461 aaaggaggat tactcctctc ttatttcaaa atcttaaaaa taaaaaatct taaaaaaaac
     5521 taacactatc taggagttta ttttgcttgt gtaactttg
//
LOCUS       DAZULY010000171         5543 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000171 DAZULY010000000
VERSION     DAZULY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.173"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(70..291)
                     /locus_tag="ACG81A_002174"
     CDS             complement(70..291)
                     /locus_tag="ACG81A_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015502.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(288..578)
                     /locus_tag="ACG81A_002175"
     CDS             complement(288..578)
                     /locus_tag="ACG81A_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015503.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(575..1759)
                     /gene="mobT"
                     /locus_tag="ACG81A_002176"
     CDS             complement(575..1759)
                     /gene="mobT"
                     /locus_tag="ACG81A_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFS5015504.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(1941..3344)
                     /locus_tag="ACG81A_002177"
     CDS             complement(1941..3344)
                     /locus_tag="ACG81A_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFS5015505.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(3366..4139)
                     /locus_tag="ACG81A_002178"
     CDS             complement(3366..4139)
                     /locus_tag="ACG81A_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015506.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(4149..4526)
                     /locus_tag="ACG81A_002179"
     CDS             complement(4149..4526)
                     /locus_tag="ACG81A_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5015507.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(4547..4861)
                     /locus_tag="ACG81A_002180"
     CDS             complement(4547..4861)
                     /locus_tag="ACG81A_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFS5015508.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            complement(5061..>5543)
                     /locus_tag="ACG81A_002181"
     CDS             complement(5061..>5543)
                     /locus_tag="ACG81A_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS5015509.1"
                     /translation="RMYSGDDNGVDLYKFVTKHIFVNLNIDEKNYPSIFKHYLFFINE
                     VEDRIKNNSLSSSVSSFKKMFKSRDGITLTTAHKVKGNEYDTVIAINLLNGKIPHWNE
                     IFNVSDKGISSARKLLYVICSRAKNNLFLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI
                     "
BASE COUNT         1643 a         1094 c          937 g         1869 t
ORIGIN      
        1 ttgtaaacat attcataaat cctttcataa caaaatatat acttcctatt ttatcatgaa
       61 aaatatctgt taagcaccaa tgattcggtt aaataagttt aataacacat ctttcacgcc
      121 agctgcattg aacacaagtc cgacggcgat tagagcaata atcaagaagc caatgagttt
      181 tgaaaattct cgtttaaaac ctaaaaacag tccaatgaca acaatcgcta acaataccaa
      241 tgactgtgca ttatccaaga accagttgta caaattctgc ccaaaattca tgtgattgtc
      301 ctcctttcct taagatattt ttctcgataa tagatatagc tttttacacg ccttaataat
      361 tgaaaagggg ttaaatgctc tccatagacg gttactcgat tggtataatt attataaaat
      421 ctcaccttgt aaaaatcata ggtatagatg gtatcaccag cttgccgttt tccctcaata
      481 atgagagacg tatatttccc tgtattttgt atatacgctt gaaactcaat gccacactga
      541 aaccctaact tctttaattc ttttgttaaa aggctcatgt aatcaaatcc tccatggcta
      601 gatgttgttg ttcaattaat ttctcatgtt tttctgtcag tttggcttcc tgaatcatgg
      661 tggagataat ggttgtaccg ttgagaacat ctaaaatgct tgcgattttt aatgtagggg
      721 cgacttgatg atgtaaccag ttcaatgttc gttcaaaaga ataaggctct ggctgtgtgg
      781 ttaggcgtaa tgctccacga tttttaccga taaaccattc ccaacgttca ttagttttcc
      841 aatcactccg acgtttagtg tcgtctttat ctgcaaagcg gatatagcga ttgataattt
      901 caaaggctgt tttttccgcc tgttgatgtg ccaataaatc tttcatggct tgggtggctc
      961 ggtcattttt taaccgaatt tcaaaccgat tctttacttc tgcgtcttct aagggaacat
     1021 catttttcac gtattgctca taatctttct catatataca gaaatagact tcacttttta
     1081 atgagccaat ataaagtgtg tttcccatgt atggtttttc atcacggtga accaattcac
     1141 cactgcgata atttttaaag cttcgaaaga cggaaatgca ttcttcctgt ttacatttct
     1201 ttgccaaatc tggaatattc aaaatccctg ttttatcgtt gatagctaaa tccaagcgtt
     1261 tgaaaatacc tttatgttcc atgcaatctt gaaagaaatc ataccagctc cgatgttgag
     1321 ccaatagaaa gttttcaaat tgccgacagc cttttccttt cagttctaat aaaacacctt
     1381 tacttacatc atgagagact aggacaacaa tatcaccgaa acgatagtgt tcgggataag
     1441 aataatagcc aaagtcttcg tgtagcatat aatccatatt caaacgtaaa acctcaccga
     1501 taatttgagc cacatcattg gtagggaatc gaatccgtac atagtcgaat aacatctcca
     1561 atgggttatc gggattgaag cgttccaaag cgttcagtaa atcttctttc acctgttgag
     1621 tagggactgc cttgcctgtt tcaatatccg ataggtaagg tctggtaata tgagaagcaa
     1681 ccgccagttt attttgagaa acgccataag ccagtcgctt ttctttcaat tccttaatcc
     1741 aaattttttc gttttccatt cgcaaacctc caatctaaaa aagatgtaaa ctatgaccct
     1801 tgagtttaca cctttcatat tttccgcaat cctttgtacc atcaacattg gttgatgaga
     1861 attgcctgtt ttcagttgta tttgtgcccc cctgttagat agagggggct aaaggattgg
     1921 cgtggctaac gccacaccaa tcaaagcaag tctgaacctg tacctttcac ttcgtgcggt
     1981 acaggctcag attgcttttt ttgaatgact tttcctattt ctcctaagaa atcgtacctt
     2041 tttggtacaa ggggtgtata aaactcacta atcacacttt ttcctgtatc cacgtaacct
     2101 cttcccttga tgggttttaa gaaaaattgt ttttgtgtat cgcttccgaa catcatacca
     2161 aagcccattt cagacatacg acccaaagcc acacggaagt taaagttgtc ccgaatccca
     2221 tcactaaaat attttgcgtc tggacgttga caggcaagaa ttaaaaagaa gcctgcttgt
     2281 cgtcctaaca ttacaatttg tttgagttta tgaataacgg tggtactttc acgacctagc
     2341 atatccatca ttgccacata ttcatcaaaa ataagaaaat tggcagacaa accaagataa
     2401 gcataatttt ctcctgtttt atagttaggg tgttgtttca tggtttcact gcgatcaatc
     2461 atatcttcgt aaaattgatt gatacatgcc aacatatctt ctttacggta atagacatta
     2521 ttcatgaccg cccctaagtc tgccaaatca gcatttttag ggtcaagaat ataaagcttt
     2581 gcatttgtat gcaggagaga ttcaatcaat gtgagaataa aataggattt tccactacca
     2641 gtgcccccag caattaacat gtgagggaga gaatcgaact cccaatagac cgattccatc
     2701 aatttgacac gtccatcttg agctaccacg tcttcaatgc caattcgatt gccaatagta
     2761 tcataaagta ggacatactc tacataagaa tctttcaacg ttttttccac taattcacaa
     2821 tacaatcctg tttctagttt gttttctagg tgcaaaagct gatcttgata tttccctaag
     2881 gtgatttcta catgaatatg gattagtcca tctttcattc gataatatat tttaggaaaa
     2941 tggcggattc gttgtttggt tttcttgctg ggtaaatctt taaagaatcc ctctgattga
     3001 gtttcttcgg cttcatacca atgattttct aaaatcatac gagctaactt ttgacggtgc
     3061 tctaactgtt ttatagaatc tatacgatag tgataataaa gccagctgat aagtccgcat
     3121 acaaaaagag cagtgagaat actgctccaa aagtatggcg tatgaagttg atagaaaaat
     3181 gtatctaact gaactgcttg ccagttcacc gattgtaacg ctctccaatg aaaagggagt
     3241 aggacgacta gaaaaactac cagcaatgaa attagtacaa aatgatagac caagtgctta
     3301 tcgttggctt tgattcgctc tcctctgtca tgaagcaatg gcataaaaat cctcctttcc
     3361 acctatcaaa ataattgtgt attgtagata cgagcctgtt tatcaaaagt tggttgagaa
     3421 atggcttcat atttctgata agcgattcgc ttttcaaagc gtaaattttt ttctttcgga
     3481 taataatgac gtcgatagag agtgtgtcct aacagttttt tcgcttcttt tgtcaacgga
     3541 tagagatagc gaaacatgcg tccattgatt ttctcaatcc ctttgtattc gcaaaatgcc
     3601 tgggaaagcc aatgcttttt ctctacctgt tcaaagcgag cattttcctc taataaaagg
     3661 cgagcacttc gagggtgtat cttttcccat gactgcttat cacgatagac acttgtcttg
     3721 aaatacccgc agtaaaagaa gttggacgct tgatagacat agccacattt tccctcaatt
     3781 ccatctgcca gtgtatataa aaaatgacag tccgtatgtt ctttcaacca cttaattaga
     3841 gctgaaagag cttgactgcc aaaataattg gtttgattca tttctggaag aaaacacatc
     3901 tttccaattt ctaaataatc tgtggtttga agggaactgt cgggaaagag tttgcgtatg
     3961 gtttgtaggg gttgcgtacc ccagcctaat tccacaacgc ctaataattt ttcttctgaa
     4021 aaaatcccta gaaagtattt acacaatcta gggagtatct ttgaataatg gtattgccga
     4081 ataaaagaaa tggcttcatc cttcggaatt tctttcaaaa tcaatggtgt gtttgtcata
     4141 agcaacacct atttttcact ttcggacgct tcttttttat atttctgatg aggttttgtc
     4201 tgtcctttta atacaagatc gtctgctttc acgtaccaat caacgtctgc ccctcgaaaa
     4261 gtagccgtcg ctacggtatc tgccacagga ttgattaatt ccacttctgc gttgtagtca
     4321 aattctttca aaggaacaga agcggggata ctgacttgta tcatgcgtcc ttgtcctttt
     4381 gattttaagt cataggtacg ttctttaatt tcttcggaaa ccgtaccatc ttcattctgg
     4441 atacgaacct cacgacgcaa ggcggaaaat ttcaatgtgc caaatgtctg gtctttttca
     4501 atcacaatac cttctgctaa tctcatattt tttcaccttt cctttcttat gctttcacca
     4561 tatcgtctgc gtgcataatg taattggtaa atcctcgatt gccaatttta taaccttctg
     4621 cggtaatatg tgggttgatg agtttgactt tttcgtcgga ttcaaaatgt ttctcgccag
     4681 cttcggaggg aagaatgaca acaatatcat ctgctcgttg aatcgttgag tataaattgt
     4741 aactacgaga gagaatggtt ggtctgccat ttattcgacg ttgttcaatg ttcccttcac
     4801 cagcatattc taattgtcca aatgtttttt ccatattggg aatgacatat ttaagttcca
     4861 tatttttacc tatcctttct gtttttatgt ttgaatgaat cttatttgtg tgatggtttt
     4921 aaagggggga gagcgacctt ttgattcttg attttttatt ttcataagtt cacttccttt
     4981 caaaattggg taaaaaaata gacgctccgt actgagcgtc cgattcctac tatatttaaa
     5041 acaacgacta cctaggtatt ttaaatatta taatcaaatt ctattttatc taataattga
     5101 gtgggataaa gcaagtctcc tttactcgta aaataaccca cctctgagaa aagaaataaa
     5161 ttattttttg ctcttgaaca aattacgtat agtagttttc tcgctgaact aattccttta
     5221 tcagaaacat taaaaatttc attccaatga ggaattttcc cgtttagaag attaatagcg
     5281 ataacagtat catactcatt tcctttaact ttatgtgctg tagtaagtgt gataccatct
     5341 cgagatttaa acatcttctt aaaactagat acactactag ataatgaatt attttttatc
     5401 cgatcttcta cttcgttaat aaaaaataga tagtgcttaa aaatagacgg gtaatttttc
     5461 tcgtcaatat taagattaac aaatatatgt ttggtaacaa atttgtataa atcaacaccg
     5521 ttgtcgtctc cactatacat tct
//
LOCUS       DAZULY010000172         5534 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000172 DAZULY010000000
VERSION     DAZULY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.174"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..988)
                     /locus_tag="ACG81A_002182"
     CDS             complement(<1..988)
                     /locus_tag="ACG81A_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFS5015510.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGN"
     gene            1140..1835
                     /gene="vanR-A"
                     /locus_tag="ACG81A_002183"
     CDS             1140..1835
                     /gene="vanR-A"
                     /locus_tag="ACG81A_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFS5015511.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1813..2967
                     /locus_tag="ACG81A_002184"
     CDS             1813..2967
                     /locus_tag="ACG81A_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFS5015512.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3182..4150
                     /locus_tag="ACG81A_002185"
     CDS             3182..4150
                     /locus_tag="ACG81A_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFS5015513.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4143..5174
                     /gene="vanA"
                     /locus_tag="ACG81A_002186"
     CDS             4143..5174
                     /gene="vanA"
                     /locus_tag="ACG81A_002186"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFS5015514.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5180..>5534
                     /locus_tag="ACG81A_002187"
     CDS             5180..>5534
                     /locus_tag="ACG81A_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFS5015515.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSR"
BASE COUNT         1670 a         1078 c         1251 g         1535 t
ORIGIN      
        1 tatttccgat aaccgtgtat tgaaaggcat cttcgaagcc attatccaat atttcgcagg
       61 cttttgtata tttaggctgg tcgatatatt caccgactaa ggcattctta gctgttcgag
      121 cgagttcaat atccgtaaac ttaaagatcg cttttactgc ttctctaaac ggttttgaat
      181 tcttttttgg aatggaactg aagatgtttc ttaaaaaatg gacctggcat ctctgccaac
      241 ttgcgttggt aaatgactta cgaatcgcag acactaggcc tttatgggca tcagaaatga
      301 tgagttctgt cccctttagg ccgcgttctt ttaagtattc aaagaagatg gaccatgtgt
      361 catcactttc ttcattttga atcatgaagc caatgatttc acggtcgcca ccttctgtta
      421 tcccgatcgc aatatggcag cttttagaaa gcactcgatg gtcctcacgg acttttatgt
      481 agagaacatc agtcatcaga taaggataat tcgtacctga gagtgaacgg ttctgccatt
      541 cgttgaccat cgggtctaac tgctcagtca ggctagaaac aaaagatttc gatacagatt
      601 ttccacatag ctcttcaaca atctttgaaa ctttacgtgt cgaaacgccc gaaacataca
      661 tctcaagcat tgaagcgagc agtgcctttt catttcgctg ataacgctca aacaccgtcg
      721 gtgaaaattc accatcacgt gttctaggca cttttaattc gagtgtaccc acacgagtcg
      781 taaagtctcg ctcataatag ccatttcttt gactctgacg actttctgaa cgttcatagt
      841 catcggcttg aatatattct gttcgttgat tttccatcaa ttggttgaaa acagtggtta
      901 aaatattttt agaaacatca tcttttaccg aatgttcaat aatactttga acctcttcgt
      961 tgttcagtgt aaaatgtact tgggtcatgt aacgtcctcc tgggtatgtt tttgtggtta
     1021 aaaacattgt accgtaaaag ggctgttaca tggcctttta tcttttacac aattatatgg
     1081 actttatcct ataatctgat aaattattaa gggcaaactt atgtgaaagg gtgataacta
     1141 tgagcgataa aatacttatt gtggatgatg aacatgaaat tgccgatttg gttgaattat
     1201 acttaaaaaa cgagaattat acggttttca aatactatac cgccaaagaa gcattggaat
     1261 gtatagacaa gtctgagatt gaccttgcca tattggacat catgcttccc ggcacaagcg
     1321 gccttactat ctgtcaaaaa ataagggaca agcacaccta tccgattatc atgctgaccg
     1381 ggaaagatac agaggtagat aaaattacag ggttaacaat cggcgcggat gattatataa
     1441 cgaagccctt tcgcccactg gagttaattg ctcgggtaaa ggcccagttg cgccgataca
     1501 aaaaattcag tggagtaaag gagcagaacg aaaatgttat cgtccactcc ggccttgtca
     1561 ttaatgttaa cacccatgag tgttatctga acgagaagca gttatccctt actcccaccg
     1621 agttttcaat actgcgaatc ctctgtgaaa acaaggggaa tgtggttagc tccgagctgc
     1681 tatttcatga gatatggggc gacgaatatt tcagcaagag caacaacacc atcaccgtgc
     1741 atatccggca tttgcgcgaa aaaatgaacg acaccattga taatccgaaa tatataaaaa
     1801 cggtatgggg ggttggttat aaaattgaaa aataaaaaaa acgactattc caaactagaa
     1861 cgaaaacttt acatgtatat cgttgcaatt gttgtggtag caattgtatt cgtgttgtat
     1921 attcgttcaa tgatccgagg gaaacttggg gattggatct taagtatttt ggaaaacaaa
     1981 tatgacttaa atcacctgga cgcgatgaaa ttatatcaat attccatacg gaacaatata
     2041 gatatcttta tttatgtggc gattgtcatt agtattctta ttctatgtcg cgtcatgctt
     2101 tcaaaattcg caaaatactt tgacgagata aataccggca ttgatgtact tattcagaac
     2161 gaagataaac aaattgagct ttctgcggaa atggatgtta tggaacaaaa gctcaacaca
     2221 ttaaaacgga ctctggaaaa gcgagagcag gatgcaaagc tggccgaaca aagaaaaaat
     2281 gacgttgtta tgtacttggc gcacgatatt aaaacgcccc ttacatccat tatcggttat
     2341 ttgagcctgc ttgacgaggc tccagacatg ccggtagatc aaaaggcaaa gtatgtgcat
     2401 atcacgttgg acaaagcgta tcgactcgaa cagctaatcg acgagttttt tgagattaca
     2461 cggtataacc tacaaacgat aacgctaaca aaaacgcaca tagacctata ctatatgctg
     2521 gtgcagatga ccgatgaatt ttatcctcag ctttccgcac atggaaaaca ggcggttatt
     2581 cacgcccccg aggatctgac cgtgtccggc gaccctgata aactcgcgag agtctttaac
     2641 aacattttga aaaacgccgc tgcatacagt gaggataaca gcatcattga cattaccgcg
     2701 ggcctctccg gggatgtggt gtcaatcgaa ttcaagaaca ctggaagcat cccaaaagat
     2761 aagctagctg ccatatttga aaagttctat aggctggaca atgctcgttc ttccgatacg
     2821 ggtggcgcgg gacttggatt ggcgattgca aaagaaatta ttgttcagca tggagggcag
     2881 atttacgcgg aaagcaatga taactatacg acgtttaggg tagagcttcc agcgatgcca
     2941 gacttggttg ataaaaggag gtcctaagag atgtatataa ttttttagga aaatctcaag
     3001 gttatcttta ctttttctta ggaaattaac aatttaatat taagaaacgg ctcgttctta
     3061 cacggtagac ttaataccgt aagaacgagc cgttttcgtt cttcagagaa agatttgaca
     3121 agattaccat tggcatcccc gttttatttg gtgcctttca cagaaagggt tggtcttaat
     3181 tatgaataac atcggcatta ctgtttatgg atgtgagcag gatgaggcag atgcattcca
     3241 tgctctttcg cctcgctttg gcgttatggc aacgataatt aacgccaacg tgtcggaatc
     3301 caacgccaaa tccgcgcctt tcaatcaatg tatcagtgtg ggacataaat cagagatttc
     3361 cgcctctatt cttcttgcgc tgaagagagc cggtgtgaaa tatatttcta cccgaagcat
     3421 cggctgcaat catatagata caactgctgc taagagaatg ggcatcactg tcgacaatgt
     3481 ggcgtactcg ccggatagcg ttgccgatta tactatgatg ctaattctta tggcagtacg
     3541 caacgtaaaa tcgattgtgc gctctgtgga aaaacatgat ttcaggttgg acagcgaccg
     3601 tggcaaggta ctcagcgaca tgacagttgg tgtggtggga acgggccaga taggcaaagc
     3661 ggttattgag cggctgcgag gatttggatg taaagtgttg gcttatagtc gcagccgaag
     3721 tatagaggta aactatgtac cgtttgatga gttgctgcaa aatagcgata tcgttacgct
     3781 tcatgtgccg ctcaatacgg atacgcacta tattatcagc cacgaacaaa tacagagaat
     3841 gaagcaagga gcatttctta tcaatactgg gcgcggtcca cttgtagata cctatgagtt
     3901 ggttaaagca ttagaaaacg ggaaactggg cggtgccgca ttggatgtat tggaaggaga
     3961 ggaagagttt ttctactctg attgcaccca aaaaccaatt gataatcaat ttttacttaa
     4021 acttcaaaga atgcctaacg tgataatcac accgcatacg gcctattata ccgagcaagc
     4081 gttgcgtgat accgttgaaa aaaccattaa aaactgtttg gattttgaaa ggagacagga
     4141 gcatgaatag aataaaagtt gcaatactgt ttgggggttg ctcagaggag catgacgtat
     4201 cggtaaaatc tgcaatagag atagccgcta acattaataa agaaaaatac gagccgttat
     4261 acattggaat tacgaaatct ggtgtatgga aaatgtgcga aaaaccttgc gcggaatggg
     4321 aaaacgacaa ttgctattca gctgtactct cgccggataa aaaaatgcac ggattacttg
     4381 ttaaaaagaa ccatgaatat gaaatcaacc atgttgatgt agcattttca gctttgcatg
     4441 gcaagtcagg tgaagatgga tccatacaag gtctgtttga attgtccggt atcccttttg
     4501 taggctgcga tattcaaagc tcagcaattt gtatggacaa atcgttgaca tacatcgttg
     4561 cgaaaaatgc tgggatagct actcccgcct tttgggttat taataaagat gataggccgg
     4621 tggcagctac gtttacctat cctgtttttg ttaagccggc gcgttcaggc tcatccttcg
     4681 gtgtgaaaaa agtcaatagc gcggacgaat tggactacgc aattgaatcg gcaagacaat
     4741 atgacagcaa aatcttaatt gagcaggctg tttcgggctg tgaggtcggt tgtgcggtat
     4801 tgggaaacag tgccgcgtta gttgttggcg aggtggacca aatcaggctg cagtacggaa
     4861 tctttcgtat tcatcaggaa gtcgagccgg aaaaaggctc tgaaaacgca gttataaccg
     4921 ttcccgcaga cctttcagca gaggagcgag gacggataca ggaaacggca aaaaaaatat
     4981 ataaagcgct cggctgtaga ggtctagccc gtgtggatat gtttttacaa gataacggcc
     5041 gcattgtact gaacgaagtc aatactctgc ccggtttcac gtcatacagt cgttatcccc
     5101 gtatgatggc cgctgcaggt attgcacttc ccgaactgat tgaccgcttg atcgtattag
     5161 cgttaaaggg gtgataagca tggaaatagg atttactttt ttagatgaaa tagtacacgg
     5221 tgttcgttgg gacgctaaat atgccacttg ggataatttc accggaaaac cggttgacgg
     5281 ttatgaagta aatcgcattg tagggacata cgagttggct gaatcgcttt tgaaggcaaa
     5341 agaactggct gctacccaag ggtacggatt gcttctatgg gacggttacc gtcctaagcg
     5401 tgctgtaaac tgttttatgc aatgggctgc acagccggaa aataacctga caaaggaaag
     5461 ttattatccc aatattgacc gaactgagat gatttcaaaa ggatacgtgg cttcaaaatc
     5521 aagccatagc cgcg
//
LOCUS       DAZULY010000173         5520 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000173 DAZULY010000000
VERSION     DAZULY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.175"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..1104
                     /locus_tag="ACG81A_002188"
     CDS             22..1104
                     /locus_tag="ACG81A_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFS5015516.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            1104..1907
                     /locus_tag="ACG81A_002189"
     CDS             1104..1907
                     /locus_tag="ACG81A_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFS5015517.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            1977..2717
                     /locus_tag="ACG81A_002190"
     CDS             1977..2717
                     /locus_tag="ACG81A_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS5015518.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            2728..3732
                     /locus_tag="ACG81A_002191"
     CDS             2728..3732
                     /locus_tag="ACG81A_002191"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFS5015519.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(3777..>5520)
                     /locus_tag="ACG81A_002192"
     CDS             complement(3777..>5520)
                     /locus_tag="ACG81A_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015520.1"
                     /translation="AYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIP
                     TVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLA
                     VIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAV
                     FIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLS
                     HLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIA
                     NIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINW
                     STILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTL
                     RLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKL
                     AAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQ
                     EEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFID
                     RITVQLSRNYPNPV"
BASE COUNT         1767 a          916 c         1133 g         1704 t
ORIGIN      
        1 tatagaaatg ggggaaaaca tttggataca aaagaagaac agctacttat tgatttaact
       61 tctgcaaaag gagtacctgg aaatgaagaa gaggtaagag aagtattcag agaatatgcc
      121 aaaccattcg ctgatgatat tttttatgac ggtttaggca gtgtgattgc caaacatggt
      181 gctggcggag gccccaaggt ctttatttct ggacatatgg acgaagttgg ttttatggta
      241 acaaaaataa ccgaaaaagg attcttggaa ttccaaactc ttggaggttg gtgggggcaa
      301 gtcatgcttg ctcagcaagt tgaaatcaaa acacgtgaag ggaaaatcgt ccacggtgtg
      361 attggttcca aaccaccaca tgttttaaca cctcaagtaa gaaataaacc ttatgaaata
      421 aaggatatgt tcattgatat tggcgcatca agccaagaag aagccaaaga gtggggcata
      481 cgtcctggag atatggtcac tccatatatt gaatacaaac gaatgaatgg cagtaaatat
      541 ttactggcaa aagcatggga taatcgtatc gggacagctg tttctctgcg agtattagag
      601 aatctatcta aagaagcaca cccaaatgtt ttattcgcag gaagcgatgt gcaagaagaa
      661 gtaggcttgc gaggtgctcg aacaagtaca caccttgtaa atcccgatat tgcttttgct
      721 ttagatactg gtacagcagg agacactcca ggaatgacac caaaagaagc agattcgatt
      781 ttaggaaaag gcccacagat tttgattttt gatgcatcta tgatcccgca taaaaaatta
      841 ttgaattttg ttattgatgt agcagaggaa atggatattc cattccaata tacagtgatt
      901 tctggcggag gaactgatgc aggacaaatg catttgacaa gagatggtgt tccgtctgta
      961 gccattaccg ttcctgttcg ttatctgcat tcccatacat cagtgattca tgaagatgat
     1021 tatttgaata cagtgaaact agtcacagaa ttagtgaaac ggcttgatag tgaaacagtt
     1081 gaaaaaattc ggagttatga ataatgaata atagaatacc tatttctgaa tatcggaaaa
     1141 aagcacgagt ttctttggaa aatcaatggg gaatcaatac ttggcttgtt ttcttatcta
     1201 taatatttac gagcgtcatc cagaactttt ttgacggttt cagtcctttt ccggaagaaa
     1261 gcatgcagca gcacttattg agttttctag tcgataattt acttttattc gcattcaact
     1321 atgcattgtt ttacattgcg ctagtagttg tacggggtgg cagaccaaaa agtaaccttt
     1381 tattttcagt tttccaaaga aagtattttg gttcgatact tggcatcaat ttgatcaatt
     1441 cagtcatcaa ctggctactc aacagtttaa tctttctgcc catgttttta atcagcggag
     1501 tgagtactta tatgcagctg cttttcagtt caaatgcggt acctacgggt gtcatgccag
     1561 aagattttct tgatatcagc tttgttttat cgatgatagg gatgattttt gtttcagcac
     1621 tcatcatgag tattgttggc ggtttattcc aatttgctgc atggacaaag ttcgactatc
     1681 ctgatttgtc ggttattcaa tgtttgaaat atggctggtt tttattgaaa gatagaattg
     1741 gtacgtatat tttgttgcag ctgtctttta tcggctggta tttgctagga gcaatcgctt
     1801 tgttcatcgg tcttttgtgg gtcattgttt atgtgaatgt gtcagttgca gaattttatg
     1861 aacaagcacg gattgaaaaa ggaggtccag acgcctattt tacttagcat gtaagcagtt
     1921 tattcatcaa aaaaataact tttgtggtag gataaatctt tgagggggaa tgtacgatga
     1981 aattaactgt tttaggatgc ttaggagcgt atccttataa aaatcaaggg acaacaggtt
     2041 atttgcttca atctgacggg ttcaatttgt tgatcgatgc aggtagtgct acgctgatca
     2101 aattacaaga acaccttgat ccacttgatc tagatgctgt gatccttagc cattatcatc
     2161 atgatcatat tgctgacctt ggtgtattac agtattactg gcaacttcat cctaagagaa
     2221 atccggagac attgctttca atttatggac acacaaaaga ttccattcac tttgaagaat
     2281 tgacgttgga aaatgtctca aaaggacatc cttattttga agctgaagaa ttaaaaattg
     2341 gtccttttct gattactttt atggaaacaa tccatccagt agtatgttac gccatgcgta
     2401 tcgtagaaga aacaacgggg aaagtattcg tatttacagg tgattcaggt tatttggaaa
     2461 gtttcgctga ttttgcgaaa gatgctgatt tgtttctagc agatacctat ctttttgaag
     2521 gaaatgaaca tcatcatgcc catttcactt caaaagaagc tggagaactt gcgaaatcag
     2581 ctaacgtaaa gaaacttgta cttacacacc tacctcagtt tggtgattta gaacaattga
     2641 aaaatgaagc gcaaaaggca gcaggcaaag ggattgatat agaacttgct gcagttgaca
     2701 aagtgtttaa catttaggag gaagaacatg atttttgtac caaatgaaaa ccacgaccca
     2761 agaatcaatt tagcaattga acaatatttg ctgcaagaaa tgcctttaga tgaacctatt
     2821 ttgctctttt atatcaatga accttcaatc atcatcggca gaaaccaaaa tacgcttgaa
     2881 gaaatcaatc gagattatgt ggaagaaaat ggcattcatg ttgtccgtcg cttaagtgga
     2941 ggcggagctg tctaccatga ttttggaaac ctgaacttta gttttatcat gcctgatgat
     3001 ggggattcat tccgtgactt tgcgaaagta acacagccaa tcatccaagc acttcatgaa
     3061 ttgggtgttg ctggggcgga attgaaaggc cgaaatgatc tagtcattga tggcatgaaa
     3121 ttttctggca acgcgatgta tgctaccaac ggtcggatgt ttgctcatgg aacaattatg
     3181 ttcgactctg atatcaatga agtagtcaat gcattaaaag taaaaaaaga caaaatcgaa
     3241 tcaaaaggaa tcaaatcgat ccgttctcgt gtaacgaata tcaaaccatt tcttccagaa
     3301 gaaaagcaaa aaatgacaac agaagaattt cgtcaagaaa tcttgttgaa aattttcggc
     3361 gtagagtcag tagaagaagt aaaaacgtac gaattgactg atgaagattg gaaaaaaatc
     3421 aatcgaatct ctgaggaata ttatcgaaat tgggattgga attacgggaa gtctccagat
     3481 ttcaatttct ctcgccaaaa acgtttttct atcggttcga ttgaagtcca tttgaatgtc
     3541 tcagaaggaa tgatcaaaga agcaaaaatc tttggcgatt tcttcggact aggagatatc
     3601 tctgacgtgg aaaaaaaatt ggttggacag aaatatgata aagcatctct atcaaaaata
     3661 gtcagccaaa ttgatgtgaa gaaatatttt ggtgcgatag aaccagaaga attgcttacc
     3721 ttactttatt gaaaataaaa aatagcggaa tccacatata tggattccgc tattttttat
     3781 acaggattcg ggtaatttcg agagagttga acagtgatcc gatcaatgaa agtatctttt
     3841 tcctgcgtta gtgttcctgt gtaacgatcc gctacgtctt tcatcatcat taagtcaaaa
     3901 cgttcctgat catctaattc ttctgtgatg cctcgactaa ataggtttgt atcgtaatgt
     3961 gcactatgtt cgcaacaaat gacgaaatgt tcctcttgtt cgtgtatcga caaataaatt
     4021 tttcgataca gtggattttc gatggcatga gtttctctaa aggaatgctc taaagcgtgg
     4081 atcaaaagtt gagccagatc gtcatcgttg ataggaagtt cttcgggaag atctgcttgg
     4141 aaggttatct gaatattccg tttggcagct aacttctggt aacgcgctaa gataagattt
     4201 gtcacctggt ggttgcaaaa agaacacagc atttcgaaat tttttgcttc ttctaatatc
     4261 ttttccacat agtttttagc ttttgtttgg tccgtatgca ttaaatcttg cagattctcc
     4321 atctcttgga caaattccag gcgaagtgta tttacttctt ggatatgctg tcgaagctgc
     4381 tcataatgtt gctccaacaa atcatttttg atttttaaga aatctacttg tctccgatct
     4441 ttgtcggtat ttaagatgtg atcgattaga ttgtagctga taataacgat tagaatgata
     4501 atatataaaa tcgtagacca attgatcgtt gtttgataga tacctaatgc cgtttggatg
     4561 taaatcaagc aatggataat ggctaccaag acgatccaag gtgtacaagc tacataaaaa
     4621 cggttattct tatatgcttc gccaatgcat gcaagtgaag tgactaaagt gcttataaca
     4681 ctagcaatat tagcaatcgg cattaattca ggaagttctg caggagtaaa agttgcataa
     4741 agcagaacgc cgataaaaat caaaagttgg aacgtacaaa aaattccgta ccatttttta
     4801 tagtaaaaca ttcggctcca atagtagcag atgatgaaag ctgacgttaa gataataaat
     4861 aaatgagata aaaaagaatg aacgaatggt tcaaaaagaa taccacttaa aatatcttct
     4921 gatacagctt caaatcctaa tgccaaagca aagcaactta agagaataag tgagtattca
     4981 agacgttttt gtttatacat caagtagatc gtatagaaaa aggtccctat agcgataaaa
     5041 acagcgatta tcatcgtcaa tacttgcggc actgattgtg aataaataaa actaatcaat
     5101 ggtccagttg ttcctagata aactttcggt ggcagtccag catagttctg ataaggagat
     5161 gagactgtaa gggtcaattt ttttccgata taatttgctg gtaaatctat cacagccaat
     5221 gagagtccgg gattgctttc ttcctgataa tctttccgtt ggtataaaat cttttcgcct
     5281 agatatacag taatccattg atggtttcct ttgattagta aatttgcttc gtccaacggt
     5341 ttttcaagtg tcgtttccat aatgaatgtt tcgtctttat ccacggtagg aatcgtacga
     5401 gtgtacttgc tgtcaaactt aaaatcagga tcatcttttg tatagaaaac ccacgaatct
     5461 tttatttctt tcatttgatt gctgtccgca ggagaaaaaa taaaataaaa gaaataagca
//
LOCUS       DAZULY010000174         5498 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000174 DAZULY010000000
VERSION     DAZULY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.176"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1078)
                     /locus_tag="ACG81A_002193"
     CDS             complement(<1..1078)
                     /locus_tag="ACG81A_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HFS5015521.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQFAYSAHTSCDDNGFILSTIITPGNIYDSQVAFQLVKQSKRLFPEIN
                     CVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQ
                     MLAFSTVTREGYREYKSNPKECVNC"
     gene            complement(1050..2249)
                     /locus_tag="ACG81A_002194"
     CDS             complement(1050..2249)
                     /locus_tag="ACG81A_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS5015522.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(2379..3755)
                     /locus_tag="ACG81A_002195"
     CDS             complement(2379..3755)
                     /locus_tag="ACG81A_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5015523.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(3755..4411)
                     /locus_tag="ACG81A_002196"
     CDS             complement(3755..4411)
                     /locus_tag="ACG81A_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5015524.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(4421..>5498)
                     /locus_tag="ACG81A_002197"
     CDS             complement(4421..>5498)
                     /locus_tag="ACG81A_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5015525.1"
                     /translation="NQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQRINREDLSD
                     EFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAKQNNLKLGDE
                     VDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDYSTSQEILNN
                     SENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEESLESVSGIKY
                     IIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQFIFELIFISI
                     PSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQSYLILISII
                     VLSVVFASSLMLIKKPKEILSKIS"
BASE COUNT         1700 a         1019 c          750 g         2029 t
ORIGIN      
        1 gacagttcac acattctttt ggattggatt tgtattctcg gtacccttct cgagtaaccg
       61 ttgaaaaagc gagcatttga tcttgcggac aaatataaca atcaaaatac tcgtcataga
      121 gaaactcatt ttttgataat agcccctttt tccctttggg ccttgaataa ggcaaacaag
      181 gtcgaaggtt caaatgagtc aaaaagtgaa caaacttagg tgtcttatac ccagcatcag
      241 cgacaacaca attgatttca ggaaaaagtc tcttcgattg tttgacgagc tgaaaggcga
      301 cttgactgtc ataaatattt ccaggagtga tgattgtgct aagaataaat ccattgtcgt
      361 cgcatgaagt atgggcagaa taagcaaatt gtttttctcg ttcgcctttg acgtagtagc
      421 cgctttccgg atcagcttta cttatttttc tttctttcag cttcacttcc ttttctaagt
      481 ccaaagtaaa agggcgtttt ccagcctcta ttcgttgacg attgatctca tcttctaatt
      541 catcctgata ggctttcgct tcatcatggg tcatttcttt cgtaaactta tgtttattgg
      601 cgttagcttt gatgtgggta gaatccagat acaaattatc ctctgttaca aaacctgctt
      661 taactgcttg ttctaaaata taggcaaaaa tatcctcaaa caccgtcgtt tctctaaatc
      721 ggcgaacata attctttcca aaagttgaga aatgggggat tttctcctcg aaggagtagc
      781 ctaagaacca gcggtaagcg acattggtat cgacttcctt aatggtttgt cgcatggaac
      841 ggataccgaa taaatactga ataaacacca atttgatcag aatcactgga tcgatacttg
      901 ggcgacctaa atcggagtaa gtggcttcga cgattggata aataaattca aaatcaagcg
      961 cggcatcaat ctttcgaacg agatgatctt gaggaactag atcatcaatc gtaagcatcg
     1021 caaattgatt tctaccattc gtattgtttt tagacatcat agctatcact ccttgcattc
     1081 gttttcataa atctattata aaagaaaaga ctgtagacat catccataaa atggactttg
     1141 tctacagtct gcgcattctc attagagaac actccctttt ttgtgacttt tctgaagcta
     1201 gagtgaatac tttcaactgc attagtagtg tacatgattt ttctaatggc gctgccataa
     1261 tcaaagagtt gttcaacatg agaaaagttt cgtttgcata catctactgc accagaataa
     1321 tgagaccacc gattttgaaa actgtcgaag gcagcgtgtg cagcgtttaa agaagaagca
     1381 ctgtagaatt ttttcatgtc tcgacagact tccttataat ccttacttgg gaggtatcgt
     1441 aacgcatttc gaacaagatg aacaatacag cgctgcacaa tgacagaagg aaaaattgct
     1501 tttgcgcctt ctttgagacc ggaaacaccg tccatcgaaa tgaaaaaaac atcttctaca
     1561 ccacctactt tcagttcatc aaatacttgc atccagcgat ttttagattc tgtttgattg
     1621 agccataggc ctaaaatctc cttgtttcct ttaagatcat agccaagaat ggtgtacaca
     1681 gcatattctt tagcttcata gttttcgcgt aaagtgacgt acatacaatc aacgaataga
     1741 aatgcataac acttggctaa tggccgagct tgccattctt ccaattcagg aaggacggca
     1801 tccgtgatat cagaaatcat ttcgtgggaa atatcaaagc cgtaaatatc ttcaacggtt
     1861 gcggcgatat cgcattgact cattcctcgt gcatacatgg aaagaacctt accttcgatg
     1921 tcggagacat ctcgttttct attaggaatc aactcaggtt caaaagaagc ctcccggtct
     1981 ctgggaacat caatgtttac tttaccaaag ctcgttttaa gcgtcttatt gccataaccg
     2041 tttcgtcgat tgtcatgttt ctttggctct ttagaatggg cgtcataccc taaatggtta
     2101 ttcaattctc cttgaagcat tttttcaaat aagggaccaa acacatcttt taaaacatct
     2161 tgcatatcct ctacagattc gggttgataa gtattcaaaa tggattcagc taacttctca
     2221 gcatcaggat ttcttttctt tctagccatc gtgtaatcac ctcttgatct tattttagaa
     2281 aaataaaaac cgtgaagcaa ccacccgcct aggattgatg gttacttaca cggtttatat
     2341 tacactctca tttttggtgc taaaaagcac cgaaaatttt aattattgtc aattaataat
     2401 tcttttggat tccttcttaa tatattgaat gaagaaataa tcaatgatac aagaagtact
     2461 aaactcataa atataactac ataaatcatg aatttaggta atacattgat atctaaactt
     2521 gataatgtct tgttaaatcc ttcagcttct gccccaccac ctaattggct agatgcagat
     2581 tgtttagcta tttgtttagc tatatcacca gtaaccttat tcaatatatt attcccaagc
     2641 ttgtcagctg tatattgagc taagaaatag gaaccaacga atgcggggat tgatataaat
     2701 accatctcaa ttaggaattg accaaaaatt tctaattttg atatacctaa tgatagtaat
     2761 actgctattt ctttctttct agcattcatc cataagaata ataataatga aactacaact
     2821 cctgcaaata ttaatgagcc aaaaaataat ttatttgaaa ttgagtaaat acctgatata
     2881 gattgttgta atgcagggta atttgatgaa cttttaatca aattatattc tctccaattt
     2941 atatctaatt tcccaagatc ttttattaca ctatcaagat tcttatcacc ttttacaaag
     3001 aatgttgcat cttggtatac agctgtatct tcggtattac cataaacttt agcagctgta
     3061 tgaatatcag tgattaatgt attttcgtat agttcttgtg ctgcacttac tccaccgcta
     3121 ttatgcccat cgaatagacc tttaatttct acttctactg tttcatctgc acctttctca
     3181 ttatcagcat caaataaatt agattttatt tttatcttat ctccaacttt aaggttattt
     3241 ttttcagcta aatctttatg cattaagatt ttatttttat cttcattttc taaatgcttt
     3301 ccttctacta atttatatgc ttctgataca aattttgttt cttttgttga gtcgttaacc
     3361 ccagttaaca taactgttct tttaaaattc tttgctcttt caggtgattg attcgcaaga
     3421 gtctcttgag tttcaataat atcataatca actaaatctg caacactgtt tattcttttt
     3481 acataggagt ctatactatc tgtttgagat atctttttaa tatcttcacc ttttacattt
     3541 cccccacctc taggtgttcc tggatttact tgtctattta tctccataga aaaactattt
     3601 gttatattag caaatgtttc ttttgaagct ctgtccgtag catctttaat agataaacta
     3661 atgagactta aagttgacat agataaaata actaatatga taattaatga ttttaaactt
     3721 tttctagtta cataagcaaa tgcatttttt atcattattc aacctccaaa ttcattttat
     3781 ttactttttt caacttttta ccacttagtt ctaaaatgat atccgcagaa tctgctactt
     3841 ccttactatg tgttacaact attacacatt tatttctatc tttagctaat gtctttaata
     3901 tattaattat ttctccagca gtaacactgt ctagattacc agtaggctca tcagctagta
     3961 ttattggagc atctgatacc aatgctctag caatagctac tctttgttgc tgtccaccag
     4021 ataatttcat aacatttctt tttatttgtt ttttatctaa acctagttca aacaagatac
     4081 tttcatctgc tgatttattt actaatctaa tattttcaat tggtgataaa taatctatta
     4141 aattatagtt ttgaaatact aaagatatat tattttttct atgattacta tatcctttat
     4201 tttgtatatc ttcattctta aacaatattt ttcctgtttg aggtttatca agtccagcaa
     4261 gtaaggaaag aagtgtagat tttcctgttc ctgactttcc tactatcgca taaaacttcc
     4321 caagttcaaa tttttgattt actcctgaca aaactttttc tttagaattt gcataactat
     4381 atgctacatt ctttatttct aatatatcca ttattttctc ctaacttatt tttgataaga
     4441 tttctttagg cttcttgatt aacattaatg aagaagcaaa tacaactgat aaaacaataa
     4501 tacttattaa tattaaataa ctttgtccaa gtgttgttat atttgacata aaactactat
     4561 tatttattaa acttccaccg gaaatcattg aatcctctga gttaatgaat ccatctacaa
     4621 ttacttttag taatacattt cctaaaaata aggaagatat tatacttggt atcgatatga
     4681 atattaactc gaatataaat tgcattataa tttgtatctt agatgttcca atagataaga
     4741 atatacctat ttcataaatt ctttccctta accatagaat caagattaat gaaagaacaa
     4801 ccatcccacc taacataata gaataagtca ttacttttat tatatatttt attccactca
     4861 ctgactctaa agactcttca aacgcattag aatctttttc aacaaaatac tttgactcat
     4921 caattttaag ctctttcaat ttgtttaagg ctaagtctgt agattctgca ctaccagaat
     4981 acattaaaat tttatttgca atttcattat tttctgaatt atttaatatc tcttggcttg
     5041 ttgaataatc tacaaacacc atattttcac taaaatcaga agataatcct gtatatgttt
     5101 cctgtttttt accagaaaag atccctataa ttttaaattt atgacttttt atttttccac
     5161 ttttttcaat atctagtaat tcaagatcaa cttcatcacc taattttaga ttgttttgtt
     5221 tagcaaattc ttcatgaaca ataattgaat tcttatcatt ttcttctata tttttacctt
     5281 ctttaattgt aaatactcca ctactaaata aaatatttct tttagtatta tttgtagctt
     5341 cgagtgaaac aacattctta aattcgtcag ataaatcttc tctatttatt ctttgttctc
     5401 cgctaactac tttagcatct tttagttttg ctaatccatc atattgaatt attttttctt
     5461 caatttcttt taatttttca atatctttaa attgatta
//
LOCUS       DAZULY010000175         5485 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000175 DAZULY010000000
VERSION     DAZULY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.177"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            11..232
                     /locus_tag="ACG81A_002198"
     CDS             11..232
                     /locus_tag="ACG81A_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015526.1"
                     /translation="MHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIKPFLEIGDE
                     PGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            295..717
                     /locus_tag="ACG81A_002199"
     CDS             295..717
                     /locus_tag="ACG81A_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015527.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(811..1128)
                     /locus_tag="ACG81A_002200"
     CDS             complement(811..1128)
                     /locus_tag="ACG81A_002200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015528.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(1155..1361)
                     /locus_tag="ACG81A_002201"
     CDS             complement(1155..1361)
                     /locus_tag="ACG81A_002201"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015529.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(1342..1524)
                     /locus_tag="ACG81A_002202"
     CDS             complement(1342..1524)
                     /locus_tag="ACG81A_002202"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015530.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(1538..1888)
                     /locus_tag="ACG81A_002203"
     CDS             complement(1538..1888)
                     /locus_tag="ACG81A_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015531.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(1914..2216)
                     /locus_tag="ACG81A_002204"
     CDS             complement(1914..2216)
                     /locus_tag="ACG81A_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015532.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(2219..2452)
                     /locus_tag="ACG81A_002205"
     CDS             complement(2219..2452)
                     /locus_tag="ACG81A_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015533.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(3356..3886)
                     /locus_tag="ACG81A_002206"
     CDS             complement(3356..3886)
                     /locus_tag="ACG81A_002206"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015534.1"
                     /translation="MKMTPNHLRHICCEVTLFNRQRWKLSNRIVRRIIVDSTGKSTVI
                     LTKKLATKQDFQLFFSQVKTIFFDGKIIANRGCSNQDLMVSNIVFSRMKRRQDLLIQA
                     RKAYKVFKKQEKQSRGKQNEQIMNKKCIEVLNGKNDHSDTKKKEGRHENYPILLVCPN
                     CFSCGFVGRIGFRTWN"
     gene            complement(3883..4998)
                     /locus_tag="ACG81A_002207"
     CDS             complement(3883..4998)
                     /locus_tag="ACG81A_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS5015535.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(5004..5402)
                     /locus_tag="ACG81A_002208"
     CDS             complement(5004..5402)
                     /locus_tag="ACG81A_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS5015536.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         1586 a         1128 c          735 g         2036 t
ORIGIN      
        1 tttaaatgaa atacatgatt gggctcaaaa taatttactt aatcgtaaac aagctgccaa
       61 aatcactgga caaaactatg atgctttttc tagctcttta cgtagaaata atatcaaacc
      121 atttctagaa ataggcgacg aacctggtcc ttccatcatt cgcttatatc taagagaaga
      181 tattgaaaaa tacgcaaaac agctcgaaga aaaaagacag aaacgtcaat gaggcgtttt
      241 ctgtcttgga tttacaagta aaacaataat aaacactttt acaacggagg ttctatgaat
      301 aagaaataca ttgtttcagt tggttcaatc gtactgattg tactcgctat tattgctttt
      361 tctgtatcca acaaaacaga aacacagcca caaaatacgg ttcaatcgac tactacaagt
      421 caatctagcg atcattccgt ctatccttca ctaaatagtt taaaagaaac gcatggacaa
      481 gatttaattg atcttggttc ttctcaaatt actactgtct tatttgaaaa gaaaaatatt
      541 gcgtttatta ctcctaaaga aggaactgac cttgaatttt ctatcaaagt aacaaatacc
      601 aaagaaaatt tattctccat tcctgacatt caatataccc ctaacaagtt aaagattggg
      661 gatacgtttg gtttggctaa agtaaatgaa caatactttg cttactcact gaaataagtt
      721 tctaatagca agaggaaaaa ccctcttgtt atttttatca cataatcatt ttataaaaac
      781 gcttcgaaaa tatcaacatt tttgaagaaa ctatttttct aatttctcaa tttcttccaa
      841 gggattttcg ctaatgaccc atacaggcac agattgctct cgaggctccc cttcttctaa
      901 ccaccaacct gcataatgaa cttcttcctc ctcagcatta ccatgatacc aaccaacaaa
      961 ggattcatct acaacgaatg aaatttcttc ttctggatca aataattcat ttttccatgc
     1021 ttcaattgct tgttctttgg tgtacttttg tttgttaaat acaccacaaa tttcgtcatc
     1081 tacataaaaa atttcaaaat caaaattact cgttcctttt actggcatct taacttttcc
     1141 tcctttgact cacttcaata gatataccac tcatgttcat tccattgttt actagttaat
     1201 tggagtttta tcttagggct ttgtgcactc tttccaaaaa cttgagcgtc gtttttttct
     1261 tctaaccaaa aaaggtaatt tcttacattc ggtactaaat gtttttcctg catttcttca
     1321 tgaaaaacat ataattctgc ttcatttttc aaccaattca ttattcattc cctcattcac
     1381 ttcttcatac cgccattcag atagtgagat ttctttttgt ttctccactt gtcctataaa
     1441 aatcgctgta ttcaacaagc tgatcacact taataataaa gcaatgaaac taatggttac
     1501 acttactctt tcacattttg tcattttgtt tccttcttta ctcgtttgtt atttcttgta
     1561 ataggtctaa taactccttc accgaaattt gcaaatggtt cgcaaaaatt tcaataaaag
     1621 cactcgcaca cattaccaga ccttcaactg atatatcact gcgacaataa aattctggag
     1681 aaacactgat ttctgattct ttcttacaat tgaacgaaaa aactattttt gaatcaaatt
     1741 ccggtaattc ttccacaact atgttttggg attctaccaa gaaagggagt gcctgttcta
     1801 atgcttcttc tggtgtttga gcagatagaa taattgcttc ttgttctttt atttcattgt
     1861 tctcaaatac tttataactt tttttcattg ttagttcctc tttcattcct ctctcaatta
     1921 gctaagccgt tactggtacg ccattctcta aatttagtat cgctaatcct taatgctcgt
     1981 cgaatatcaa tcattcgata acctattgca cgaagttctt tatatctttt agcagttagc
     2041 gtctctaacg tcatttcagg caatgggttc gaatcaaaaa tagaattcga accgattatt
     2101 tgggccaatt gttgtacttg taacagctct tctgaactta attcagtgat tgctcgattg
     2161 cctatcattg catacatttt ctgtcgaata tacttctttt cttgtttaga caacatttct
     2221 atccactcct attttcatta tcatttgtta cctccacttc agtaaatcca caaataaatg
     2281 aattcatatt ccaagtacca ttcccttcaa ctacaacttt tctcctgtcc tctttcggat
     2341 attcaagaat cagaccgttt accaatatgt ttttcactac tactaatttg tttgtatacc
     2401 gtggaattgc tgttccgata tacttcactt tatttcctac gctaattgtc atttcctcac
     2461 tcctattctt ttttatccaa aactttttcc tgtcctattc ctgccaacga atatacttca
     2521 ctgcatgctt aaacaaaaca aaatgttgcc ctgttttatt tccgtctttt acttgttcca
     2581 catacagttc atatttggtc acacctttta aaacgccaga tacttttttt catccattgt
     2641 cacaattagt tttttcatca atcgattgat actgcactgc tcatttttct tgttcatata
     2701 gtattccttt ctttttcaaa actagcctct tttgtttact agttttcatc aatcatcaac
     2761 gatttaacag attttttctt tcgcagggtt tctagcgtca gcaaccccaa actctaaaaa
     2821 agtgcctttg ctcccttgaa aaacgagcgg acgattgtac tcgtcatcat atgtttttca
     2881 tactacctga tccacgcaaa taaccttttt ttgttctgtg ccacacccat ccatacgtat
     2941 cgcttgagat tcaccatact tttttaggtg gtttatcctt cctaagtccc tgttagacac
     3001 gctgattaac ttagaaagtc ctgtttgtat ccaattttca aagatctccc aaaagaaata
     3061 agtaggttta cgtgtatcgc ttctgttcaa agtacctgtt tgtacttgag tatttctact
     3121 cccctcgttc aagggattcc ttccaccagc tagcttttcc tttcatcatc aaatgttcct
     3181 gaaaagcatt tacgtttcgt gtccgtgtgc ttacttattc ttttttcgat agcgattatc
     3241 cgctcgctac tcgctatttt atactttgtt tgttagctca aataaaaact aacaaacaaa
     3301 aaaggctagt ttctttccgt tttatttttt taactgtcga taaatagttg ttagtttaat
     3361 tccaagttct aaaaccaata cgcccaacaa aaccacagga aaaacaattg gggcaaacaa
     3421 gtaaaatcgg atagttttca tgtcttccct ctttcttttt ggtgtcgctg tggtcgttct
     3481 tgccgttcaa tacttcgata cattttttat tcattatttg ctcgttttgt tttcctctgg
     3541 actgcttttc ttgctttttg aatactttat acgcttttcg tgcctgaatt aataggtctt
     3601 gtcggcgttt cattcgtgag aaaacaatat ttgaaaccat caaatcctgg ttcgaacaac
     3661 cacgattcgc aataattttc ccatcaaaaa aaatagtttt cacttgagaa aaaaacagtt
     3721 ggaaatcttg ttttgttgct aatttttttg tcaaaatcac tgtgcttttc cctgttgaat
     3781 ctacaatgat tcttcgaaca atccgattcg ataacttcca acgttgccga ttaaacaatg
     3841 ttacttcaca acaaatatga cgtaagtgat taggggtcat tttcatgtgt ccaattcctc
     3901 caaccaatta tgcatcgaaa ttttgggcaa ttgtgacagc ccataaatct gcgtttccag
     3961 gtcttcataa ctacaagaac gcttggcttg aagtaatgaa tattctgctt gatctgatag
     4021 ttttgttgcc attggatcta gattcaattc tcttacacaa cattcccaaa aattcaatag
     4081 cgctctaaaa taaaatcctt ttcgattctg aattgctttt ccttcatgac gagcaatttt
     4141 ttcattttga atacaccgtt gatacgtttt gcttaaatct tctgcaaatg tttcccctaa
     4201 aattcgatac tcgcttccat cagataaacc atgtgtttga ttatactttc gttgccgctt
     4261 ttctataatt cgtttactgt cgtatatcat gtttagctgc tctttcgctt cacgccattc
     4321 agctgaactg cgaacaatcc atgtcaaaac atctttcgga acaaacgtac tttcgctgta
     4381 atgagcaata aactcttttt gccaatcatc agccatttca acaggactag tcgtatcgat
     4441 ttttgtatct agattttctg tatctagatt gttagaagaa ttattaaata attgtgcaga
     4501 attttcacat ggattttttt cgtcaaaatc atcgaaaatc gctaaattat caacagattc
     4561 ctcctcttct tttccttgtg cagaatctac actggttgag tcaacaggac ctaatgaata
     4621 cacatcattt tctgtcgtat ctaattctcc tagatacaac cgagctttgt ttctttttcc
     4681 tgtttgttga tcaaatccca tatcctctat ttctagtagc tcatattctt tcaattcttt
     4741 tttgattgta ttaacagaac gtctgcaaca attcaatgca ttggctaatt cttgttccgt
     4801 aaattcaaaa taaacaaaac cttgactatc tacttgattt cgttgaatgg ctaaatctaa
     4861 gcgtgaccga aaaaccatat acgcgatttt tgctttatca gacaatgagc gatatttttc
     4921 acttgtaata aatacctttg gaaattgata gtataattca ttgtatttac gagttgcggt
     4981 aaatcttttt gttgccatcg ttattacacc tcatcttgtc catatattaa gaaagaattt
     5041 tggtgttggt agacacgaac atcttccatt gtagcaatca attcttttag ataaattcga
     5101 ccgaattcaa ttgttagtaa gtcgtaatct tctaattcat ttttgatttc ttcaatttct
     5161 tcttcagaca cattgagtaa aatggctaat tcttgattgg tataagaaat atagacatgt
     5221 ttcttttcat caatccgttt ttcaattaag gattcttcta agtactcatt cagtaagcta
     5281 taggcgtatt ttgcttgtgc agtcatcgca taatattttg acgattttat aaagaatagt
     5341 tttggcatat gaaaagttgc ttgtgtcagc acatctgttg cgcgaaattt gttttttacc
     5401 atattttttc ctcctagaaa aacacgcaaa aaaagctctg tataaatcga attacacaga
     5461 gtttcttctt gttgatattt aattt
//
LOCUS       DAZULY010000176         5437 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000176 DAZULY010000000
VERSION     DAZULY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.178"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      68..165
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            281..862
                     /locus_tag="ACG81A_002209"
     CDS             281..862
                     /locus_tag="ACG81A_002209"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFS5015537.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            865..2172
                     /locus_tag="ACG81A_002210"
     CDS             865..2172
                     /locus_tag="ACG81A_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFS5015538.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            2465..2953
                     /gene="purE"
                     /locus_tag="ACG81A_002211"
     CDS             2465..2953
                     /gene="purE"
                     /locus_tag="ACG81A_002211"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFS5015539.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            2946..4070
                     /gene="purK"
                     /locus_tag="ACG81A_002212"
     CDS             2946..4070
                     /gene="purK"
                     /locus_tag="ACG81A_002212"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFS5015540.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            4083..5378
                     /gene="purB"
                     /locus_tag="ACG81A_002213"
     CDS             4083..5378
                     /gene="purB"
                     /locus_tag="ACG81A_002213"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFS5015541.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
BASE COUNT         1698 a          930 c         1262 g         1547 t
ORIGIN      
        1 tttttattca aatatggcaa actgccttca cgtgcaaaac agttgcaaga aaatgaattt
       61 acactattat tagttttaca tacctactta tatatcgtca taatatggat gacagtttct
      121 agccagtacc gtaaatgctg gactataagt aaaagattgg ctatttcacc cttcttttct
      181 tatgataaag tggagggtgt ttttctttaa cagaaacata gtaggttttt gcacaaaagc
      241 ggttatttcg gtattaataa atgataaagg agcaaataaa gtgaaagaat tagttgaacg
      301 tattcaaaaa gacggacgta tattaggcga gggcgtatta aaagtcgaca gcttcgtcac
      361 tcaccaagta gaccctgtat tgatggaaca aatgggaaaa cggtttgcag aagtatttaa
      421 agaacaagga atcacgaaag ttgtgaccat tgaagcttct gggatcgcac cagctttatt
      481 tgctgcaaga gaattagatg ttccaatgat cttcgctaga aaagcaaaaa gtttgacgat
      541 ggatgaagaa ttattgacag cttctgttta ttcttttacg aaacaagtga caagtacgat
      601 ttctatttct cgtaaatttt tatcttcaga agacaaagta ttgattatcg acgatttctt
      661 ggcaaacgga caagcggcaa aaggattgat cgaattatgc cagcaagctg gagcgcaagt
      721 agaaggaatc gggatcgtca tcgaaaagtc tttccaagat ggacgtcagt tacttgaaga
      781 aatgggattg agagtcgttt ctcttgcgcg tattgcttcc ttgacgaatg gacaagttga
      841 gtttcttgag gaggacgcat aatcgtggaa aaagaaacac aaaatggaaa agctgcggtt
      901 ttagggctgc agcatctgct agcgatgtat gcaggagcag tagcggtgcc tcttttgatc
      961 ggtacaggac taggctttaa tcaagaacag atgacttatt tgatttcaat tgatatcttt
     1021 atgtgtggat tagcgacatt attgcaattg acagttaacc gatttttcgg aattggcttg
     1081 cctgtcgttt taggatgtgc gatccaagca gtagctccac tgatcttgat cggaaccaat
     1141 gaaggagtag gagctatcta cggatctatt atcgcttctg ggatctttgt tgtgttagtt
     1201 tctggtgtct tttctaaaat taaaaaacta ttccctccta tagtaacagg aacggttatc
     1261 accgttattg gtttgacttt gattcctgta gcaatcgaga aaatgggtgg aggtaatgca
     1321 acagcagctg attttggtga caaaacaaat ctgctacttg catttgtgac gattctccta
     1381 attgtaggcg tgcaaatgct ggcaaaaggg ttcattcgtt caatcgcggt attgattggt
     1441 ttagtcggag gaagcatctt agctgctttc atgggaatga ttgatctgag cgcgattgga
     1501 gatgcgccgt tgttccatgt tccacaaccc ttttattttg gtaaaccaac tttcgacgta
     1561 tggtcgattt tactaatgat catcatttcc atggtcagca tggtagaatc aacaggtgta
     1621 tattttgcac ttggtgacat tactgggaaa aatgttggag aagaagaatt gaaaaaggga
     1681 tatcgtgcag aaggacttgc cgttatctta ggcggtattt tcaatacatt cccttatact
     1741 ggcttttctc aaaatgtcgg attggttcaa ctttcgggaa tcaaaacaag aaaacccatc
     1801 tatttctctg cattcttctt gattgtttta ggattattcc ctaaaatcgg tgcgttagca
     1861 caaatcatcc ctgagccggt acttggtgga ggaatgttag tcatgttcgg gatggtggca
     1921 gtccaaggaa tccgtatgct gttggaagtt gatttcagca atgataaaaa tctattgatt
     1981 gttgctgttt ctgttggatt tgggctgggt ttcaatgtta cgccgacatt attcagtcaa
     2041 atgccgcaaa cattgcaaat gttcactgga aatggcatcg taatgagtag cattacggct
     2101 atcatattga atttgatctt taatggattg aaaaaagatg aagaaatatt ggaagaagcc
     2161 ttcaagaagt aatagagcaa aagaaaggtt gtgaaatcag cgttttgacc tgattgttag
     2221 agaaacaaac ggaaaaatag ttcctcgtat tttgccgttt gtttgagcta ataccgaggg
     2281 tcagctgatt gaacaccgtt tatccataaa aaggaatgtg acctaacttt tgtcacattc
     2341 ctttttttca tctttaataa acacgaacaa taaaatgttt ttctttgtta acattcgaaa
     2401 tttctttgac atcaaaaaaa gtgattgtta aaatatgaat gtagaaaaac tgggaggaaa
     2461 aacaatgaca gcaaaaatct ctgtgattat ggggagtact tcagattggg aaacaatgaa
     2521 agaagcatgt caaatattgg atgaattcgg tgtgtcttat gaaaaaaagg tcgtttctgc
     2581 tcatcgaacc ccagatttga tgtttgaata tgcacaaaac gctcgaaaaa aaggaatcaa
     2641 aatcattatt gcaggagcag ggggagccgc gcatctgccg ggtatggtcg catcaaaaac
     2701 aactttgccg gttatcggtg taccggtcca atcacgaaca ttaaatggtt tggattcgct
     2761 tctttcgatt gtccaaatgc ctggaggagt acctgtagct actacagcaa tcggaaaagc
     2821 gggagcaatt aatgctggac tacttgcgat acagatgctt tccatgtatg atttagaatt
     2881 ggaggctaaa ttggctgaaa gacgttcaat gttggctaaa actgttatag aaagtagtga
     2941 tcaacttggt taaacctttg ttgcctggtt caacgatcgg gatcgtcggt ggcggtcaat
     3001 taggtcgtat gatggcgatt agtgcaaaag aaatgggctt taaagtaggt gtgctggatc
     3061 ctgtgagcga ttgtccagcc gcacaagttg cagattggca cattgaaagt gtctatgatg
     3121 acacctttgc gctgaaagag cttgccagaa gaacagatgt cattacctat gaattcgaaa
     3181 acgtcagtgt agaggcgtta aatgcgattt tacccatgtc atttattcct caagggacag
     3241 acttgcttgc gattacgcaa gaccgcctgt tagaaaaatc ttttttggaa acgaataaca
     3301 tcgtgattgc tccttatgca acgatcgtca gtccgacgga tatccaagat gcgattgacg
     3361 ggatcggcta tccttgtgtt ttgaaaacca ctcgaggcgg ctatgatggc aaggggcagt
     3421 atgttttgaa aagtcgggcg gatctagctc ctgccatgga ccttttaaga gaaggaacct
     3481 gtgttttaga agcatggatc ccgtttgaaa aagagatttc gatcatggtg gcaggaaatg
     3541 gtcaaggaga tttcacgact tttccggtcg tggaaaacat ccaccacaac aatattcttc
     3601 atgaaacgat tgctccagcc gcgattgatc aggatgtgat cgaggaggct gagcgtattg
     3661 cccgtgtgat tgcagaagca gtgagccttt caggtgtttt aggggtagaa atgtttttaa
     3721 caaaaacagg cgggctatat gtgaatgaat tagctcctcg tcctcacaat tctggacatt
     3781 attctattga agcttgttct atgagtcagt tcgatgccca tattcgaggt atttgtggtt
     3841 ggccattagg agaaaaagta cgtttgataa gcaatgcagt aatggtcaat gttttgggag
     3901 aacaactgct ggaaagctat cgcttgattg agaataagcc agactggcaa ttccatttct
     3961 atggaaaaaa acaagcaaaa aaaggtcgca agatgggaca tatcactatc ttgacagaag
     4021 acatccagca aacgctggaa gaaatcaaac aaacgaatat ttgggattag aggagaaaaa
     4081 acatgttaga acgttataca cgccctgaaa tggggaatat ctggacggaa gagaacaaat
     4141 accgtgcgtg gctcgaagtc gagattttag cagatgaagc ttgggcagaa cttggtgaaa
     4201 tcccgcaaga agatgtgaaa aaaattcgtg agcatgcatc atttgatatc gatcggatct
     4261 tggaaatcga aaaattaaca cgccatgatg tcgttgcgtt tacacgagcg gtttctgaaa
     4321 cattgggaga agaaagaaaa tgggtccatt atggtctaac aagtacggat gtcgtagata
     4381 ctgcttatgg ctatctcatc aagcaagcaa atgatatttt acgggaagac ttgaaacgtt
     4441 ttacggaaat cattggcgaa aaagcaaaag aacacaaata tacagtgatg atgggtcgga
     4501 cacacggtgt ccatgctgaa ccaactactt ttggattgaa acttgccaca tggtattcag
     4561 aaatgaaacg gaacatcgaa cgatttgaac atgcggcaaa aggagtagaa gcaggaaaaa
     4621 tctcaggagc agtgggaacg ttcgcaaata tcccgccatt tattgaagag tatgtatgta
     4681 aacaattggg gatccgtcca caagaaatct ctactcaagt tctccctcgg gatctacatg
     4741 ctgaatattt ctcagcgatg gcgcttattg ctacaagtat tgaacgtttt gcaacagaga
     4801 tacgcggatt gcaaaaatcc gaaacacgtg aagtagaaga attttttgcg aaaggtcaaa
     4861 aaggttcttc tgctatgccg cacaaaagga atccgatcgg ttcagaaaat atggctggtc
     4921 tagccagagt ggtgcgcgga cacatggtca ctgcctatga aaatgtcaca ttatggcatg
     4981 aaagagatat ttctcattct tcagcagaac gaatcatctt gcctgatacc acgattttat
     5041 tgaattacat gttaaatcgt tttggcaata tcgttaaaaa tctaacggtc tttcctgaaa
     5101 acatgaagcg gaatatgtct gcaacatttg gtttgattta tagccagcgc gttatgctga
     5161 aattgatcga taagggaatg acaagagaag aagcttatga tcttgttcag ccaaaaactg
     5221 cgtacgcttg ggacaaccaa gtgcagttcc gcccgctgtt agaagaagat ccaaccatca
     5281 catcggtatt atctaaagaa gaaatagacg atgcctttga ttatcatcat catttgaaaa
     5341 acgttgacgt gatattcaaa cgagtaggtt tggaataaag aagagtgagt tgcaaaattc
     5401 aattaaaata gaatagcaaa aataaccaaa aaagaag
//
LOCUS       DAZULY010000177         5428 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000177 DAZULY010000000
VERSION     DAZULY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.179"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(72..692)
                     /gene="rpoE"
                     /locus_tag="ACG81A_002214"
     CDS             complement(72..692)
                     /gene="rpoE"
                     /locus_tag="ACG81A_002214"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFS5015542.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(768..1205)
                     /locus_tag="ACG81A_002215"
     CDS             complement(768..1205)
                     /locus_tag="ACG81A_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFS5015543.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            1368..2198
                     /locus_tag="ACG81A_002216"
     CDS             1368..2198
                     /locus_tag="ACG81A_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFS5015544.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            2182..3549
                     /locus_tag="ACG81A_002217"
     CDS             2182..3549
                     /locus_tag="ACG81A_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFS5015545.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            3568..4377
                     /gene="yidA"
                     /locus_tag="ACG81A_002218"
     CDS             3568..4377
                     /gene="yidA"
                     /locus_tag="ACG81A_002218"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFS5015546.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(4420..4863)
                     /locus_tag="ACG81A_002219"
     CDS             complement(4420..4863)
                     /locus_tag="ACG81A_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFS5015547.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            4997..5368
                     /locus_tag="ACG81A_002220"
     CDS             4997..5368
                     /locus_tag="ACG81A_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5015548.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
BASE COUNT         1653 a         1135 c          982 g         1658 t
ORIGIN      
        1 aaatcgtgtc acacctcctt attctgatag gctgatcgtc tctgtcacaa cctctcgtaa
       61 gaaaataatg tttattcttt ttcagagtca gaaaattctt cctcttcata atcttcatca
      121 tcatcgtctt cgtctatgat acttaaatct tcttcgatat ttcctggcaa gtcttcttca
      181 tcatctgtcg ggtcatctgc tccgatttct tgcaagtcag aattataagc agcgatttct
      241 tcatcttcat catcatcgtc atcaaacaga tcatcgtcat cttcttctgt cagatctgta
      301 tcttctgggt catcgtcatt gtaatcgatc gcgtcttcat catcattgat aaaggcgttg
      361 actttcttgc gtttacggcg acgtggagta tcttcatcct cttcgtccat tccatggttt
      421 acttcttcat cgatcgaatc gatagcatac catgaacgca agccccaacg attatcccct
      481 aatgagataa agctgccgtc aatattcaag tcagtataaa attgtgcgag tgaatcacga
      541 atttcactgt ctgatttccc aagatagttt tggatctggt tagctaaatc agaaaaatcc
      601 atgacatcgc cacgttgttc taagatcgcg tgtgctactt cgatcattga taattcgctt
      661 ttgtttgcac cttcaaatac actaatttcc aatcaggaca cgtcctttca cagtctactc
      721 cttatcatac aaaatcagac acaaaaaatc aaacttttct tttatattta cgctgtaaaa
      781 tccaaggtca aataatattc gccgattttt tccggtccca tgaacagatc ataatcgatt
      841 cgaaccttcc ctcctgtggg acggtctttc aaactaacat gcagttcttt cgtaaaagta
      901 gcaatctgga tcataccgta tggtgttcga taggatgttt ctaaccgttc tttatagcca
      961 aatttcaaac gcatccgcat ttctcctgca cggatcagct gtacatgacc atctggaact
     1021 aatttgatcg ttacaggaat ctcatcgccc gtctcctcct gaatctcttt ataacgtatg
     1081 tacagcgtat cgccaattgt caccatttgc cctttcaaat caaaataaaa atcttgcact
     1141 tccccttgct gttttacttt tgtacgcaat ttgattgaaa tcggtgttcc ttgtgataat
     1201 tccattattc attctccttt acttctcata gctacattgt tcctagtttc ttttgttttg
     1261 tcaaccaact tgtccatttt tacagaaaac cttttttcca aaacaactct ttgattttag
     1321 agtataatat tgtagaaaat atgaatttat acaaaggagc cggttgaatg aaccctgcga
     1381 ttcgactaga tcagcacatt tatcaaaaaa acgacttttc cccttttgca ttaacagata
     1441 tattaacgga atattctaaa gaacaccagc aaatcttttt acatttctgg caatatgact
     1501 gcacagtcat cttaggtatg aaggatgttc gaactccttt tctaagtgac gggatccaaa
     1561 cactgaaaga tgcaggttac gctccagtga tcagaaattc aggtggatta gggatcgtat
     1621 cagatgaagg aattttgaat atttctttga ttttccccca aagtgacaca aaaaaaataa
     1681 ccatagatga cgcttacgag caaatgctcc tattgactaa atacgctttt ccaaaggcac
     1741 cgatacaagc attcgaaatc acccattctt attgtcctgg aacttatgat ttaagcatcc
     1801 atggcaaaaa atttgccggt atcgctcagc gtcggatcaa aaatgggatt tctgttatga
     1861 tgtacttaag tgtcaacggt aatcaggaaa aacgcgggca agtcatgaag cgattttatc
     1921 aatctgcttt aaaagaagac tttggtacaa atggctatcc tgcagtggat cctgctacaa
     1981 tggcaacact tgaagaactg cttgaccaac ccctttttgt aaaagaagtc aaagaagcat
     2041 ttgcaaaagc ttttgaaaag ctgtattctg caagtgattt acaggattct accgactggc
     2101 taaaaaaaga aatccagctg gagcaatgga acaaccaaat ccagagaatg gaacaacgta
     2161 ataacataaa ggagctacat tatgacaacc ccttataaat atcaaatgct gcctatggaa
     2221 aaagtttttc gtgatccagt ccataactat atccatgtcc agcatcaagt aatcttagat
     2281 ttgatcaatt cgaaagaagt ccaacgtcta cgacgcatta aacaattagg cacttcttct
     2341 ttcacttttc acggagcaga acatagccgt tttactcatt cattaggcgt ttatgagatt
     2401 tcaagacgaa tctgcgatat cttttctcga aatttttcaa aagaaaaaat aggcaacgga
     2461 ggctgggatg atcaggaacg gctggtgact ttatgtgcgg cactgttgca tgatgtcggt
     2521 catggtcctt actctcatac atttgaacat atcttccata cgaatcatga agccatcact
     2581 gtagagatca tcacttctcc agaaacagaa gtgtatcaga ttcttaaccg agtagaagaa
     2641 ggttttcctg aaaaggtagc tagtgtgatc caaaaaactt accccaaccc acaagttgtc
     2701 caaatgattt ctagtcagat cgatgcagat cgaatggact atttgcttcg ggatgcttat
     2761 ttcactggaa cagaatatgg aacatttgat cttacccgaa tattacgtgt gattcgtcct
     2821 tatgaaggcg ggatcgcttt ttccatgagt ggcatgcatg cagtggaaga ttatatcgtt
     2881 agccgatatc aaatgtatgt gcaagtgtac ttccatcctg tttcccgtgg tatggaagta
     2941 attttggagc atctgcttca tcgcgcacac gagttgtatc aagaagatgc cagcagtttt
     3001 gaactccatt ctcaattact tctgccattt cttgacggaa gctttacttt agaagaatac
     3061 ctcaaacttg atgatggggt cttgggcact tactttactc aatggtctga atctgctgat
     3121 cctattttaa atgatttagc taaacgtttt ttgaatcgta aaccactgaa atctgctact
     3181 ttttctggca accgggattc aaaattagtc caagaactca ctttgttagt agaaaaggtc
     3241 ggatataatc ctgtttatta cactgctgtg aattctagct acgatctacc ttatgatttt
     3301 tatcgcccag aacaaggcag acatcgcaca caaatcgaga ttttgcgaaa tgacggaact
     3361 ttgatagaat tatcacaggt tagtcagctt gttgctgcat tagcgggaca agaacaagga
     3421 gatgagcgat ttttctttcc aaaagaaatg gttgatccgt ccttgcgtga tcattatgat
     3481 ttatttgacg aaacttatca agaatttgcc agccatatcc gtaatggcgc attgattgaa
     3541 ataaattaaa atatattgga ggaaattatg tctatcaaac tagtcgctat cgacatcgat
     3601 ggtactttat taaatgagaa aagagaagta acaaaagaag taaaagaagc gattgccgct
     3661 gctgttgcaa aaggtgtttc gatcgtttta tgcactggcc gtcccttacc aggtgtccag
     3721 gaacagttaa acgagttgaa cctttttcaa gataatgatt acgttattac ttataacggg
     3781 gcactcgtac aacaaacgaa atcaggtaaa atcatcgccc gtcacggact gacacatgaa
     3841 gatttcctag agatcgaagt gatggcacgc cgtgtcggca gtcatctcca ttcgatcgat
     3901 gacaaagcga tctacacgcc taatcgagat atcagcgctt atacgatcca cgaagcaagt
     3961 ttagtcaaga tgccgctaaa atatcgaaca cctgaagaaa tgacaccaga catgaacatc
     4021 gtcaaaatga tgatgatcga cgaaccagaa attttagatg cagcaattgc gcgtttaccg
     4081 caaactttcc gtgataaata cacaacggtc aaaagtgcgc cctattactt cgaagtacta
     4141 aataaagaag caagtaaagg tgcggcagtt gcgaatctcg cacagcacct tggtattgat
     4201 caagatgaaa tcatggctat cggagataac gagaacgact tatccatgat tgaatatgct
     4261 ggtctaggag tagcaatggg aaatgccgtt tctcttgtca aagaagcagc aaatgtcatc
     4321 acttcatcaa acgacgagca cggtgtagct gaagcaatca aaaaatatgt actgtaataa
     4381 ataaaaaaac tgagagaaag ctgatctact cgctttcttt cagtttttat ttttttcgat
     4441 ccatctctcg actgcccatc ggtggctacc acgtttcaat ttcacaagcg cttcttgagg
     4501 gctcacccac caaattttat tggtcttttc agttggttct cctatacgct tccaagaatc
     4561 agcaacaaag aaatatcctg gattataata ataagttgca cgataattcg agtaaaaata
     4621 ctcatcagct tctcccagat aagaagaaat ttctgccaca attcccattt cttctatcag
     4681 ctcacggaca atcgcttctt ctttcgtctc ttctccttca atttccccgc caggaagaaa
     4741 aaaagcacca tttggagcct cgattactgc aatctgctga tctctttcta tgacaatata
     4801 tgctgcataa cgggtttgat aatcagcaaa ttgctttttt tcaccaaaaa ttggtttatc
     4861 catgaatagt ttccacctta acagttttat tttctatcca ttttacatga gaaatttctc
     4921 atgaacaagc acaaatcaac tatttcctta cttctttttg ttatgattga gtaaaaggaa
     4981 aaggagagat gaatctatga aaatggctca cacatgtgtt cgagtgaaag atcttgaagc
     5041 atcaatcgaa ttttatcaaa aggcatttgg ttttgaagaa agccgccgcc gtgattttcc
     5101 agaaaacaaa tttacacttg tttatttgac acttcctggg gatgactatg aattagagtt
     5161 gacttacaac tatgatcatg gtgcgtatga tctaggagac ggctatggtc atatcgctat
     5221 agcagctgat gatctagaaa aattacacga gcaacataaa gctgccggtc ttgagattac
     5281 tgacttaaaa ggattacctg gcactgcgcc ttcttactac tttgtcattg accctgatgg
     5341 ttataaaatc gaagtcatcc gaggatagtt agtatggggc attttactct tgattgaata
     5401 aaaaacaaac accaataatt aaaatgag
//
LOCUS       DAZULY010000178         5418 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000178 DAZULY010000000
VERSION     DAZULY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.180"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..662
                     /locus_tag="ACG81A_002221"
     CDS             <1..662
                     /locus_tag="ACG81A_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015549.1"
                     /translation="LFSSFSAFLLIACLTLYHLQSALANQVQDRLSFIGASASTELLP
                     ALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNKQELINWRLTGLSKTKLFLFIFW
                     QLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVLKTTELPDIRDILTSVSGLTIPM
                     DQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLILSLFPFTFFIHHLQSRRPGRNE
                     T"
     gene            652..1161
                     /locus_tag="ACG81A_002222"
     CDS             652..1161
                     /locus_tag="ACG81A_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015550.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1231..2679)
                     /locus_tag="ACG81A_002223"
     CDS             complement(1231..2679)
                     /locus_tag="ACG81A_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5015551.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2679..3395)
                     /locus_tag="ACG81A_002224"
     CDS             complement(2679..3395)
                     /locus_tag="ACG81A_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFS5015552.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3376..3726)
                     /locus_tag="ACG81A_002225"
     CDS             complement(3376..3726)
                     /locus_tag="ACG81A_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFS5015553.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            3870..4643
                     /locus_tag="ACG81A_002226"
     CDS             3870..4643
                     /locus_tag="ACG81A_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5015554.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            4814..5180
                     /gene="ssrA"
                     /locus_tag="ACG81A_002227"
     tmRNA           4814..5180
                     /gene="ssrA"
                     /locus_tag="ACG81A_002227"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(5226..>5418)
                     /locus_tag="ACG81A_002228"
     CDS             complement(5226..>5418)
                     /locus_tag="ACG81A_002228"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015555.1"
                     /translation="SISIKVHLKKSVRTKVLFVNLKIDFPEYVLGISKCLNVFLNAKS
                     MQKILYFSTFLSLTKIKKR"
BASE COUNT         1562 a         1071 c          954 g         1831 t
ORIGIN      
        1 gtttattttc aagcttttct gcttttttat tgatagcatg cctgactctt taccatttgc
       61 aatcggcttt agccaatcaa gtccaagatc ggctttcatt tattggcgct tctgcatcta
      121 cagagcttct tccggcttta gaattagcta gccgatttta tataaaaggc atattcagtt
      181 tattttttct atttgccttc ctttttatct ttttctttta ctattcagtc aggcaaaaca
      241 agcaggaatt gatcaattgg cgcctgactg gtctttctaa aaccaaatta ttccttttta
      301 ttttctggca gctgttcttt cctctctttc tttgttgtat cttggtttta ttgtggatcg
      361 taagcttcca gtcagtttat gaagcgcttc ttcaaaggat caattttctt gtcttaaaga
      421 ctactgaact gccagatatc cgtgatatct tgacttcggt aagcggactt acaattccga
      481 tggatcaaca gacttttttt accatcgatt tccaaaatga tctttttttc acagatatac
      541 ttaagggatt tgcccaaact cttattgggc tcagtggatg ctgtctcatt cttagtctat
      601 tccctttcac tttttttatc catcatttgc aaagtaggag gcctggacgt aatgaaacct
      661 gaatatttga ttcatgctga acacttgacc gttattttgt gtcctctcga aaaacagcat
      721 aaaatattgg agcacttgaa aaaagaggtt cttgttgcaa aactggataa ttttgcgatc
      781 atccaaaaaa attggccgat gtttccttat ctgaatctta aagaccatgt cctgttggat
      841 gtaccggaaa aagaaatcaa acaagatcgt ttggcctatc aagaaaaact tgatatctct
      901 ccttccttgc ttaattgtgc ggcagctgag ttgacgtctt ttgaaaaagt aaagctgcaa
      961 ttacttcatg ccttgctttc caagcgaaac aatattgtta ttgaagatac atttgatgaa
     1021 cttagtgtat tggagatcca agagctactc cacttgcttt cttatttagc tcatgaagaa
     1081 aatcaaggta tcttactctt tactcatgat gctactattg ctcaaagtcc ttatattgat
     1141 cgtttagagc ccgcgggcta aatcaatcat tttatagtaa aaacaagcgg tatagaagat
     1201 cgattcatga tcttttatac cgcttgtttt ttactctttt ttgatttcta cgtcaggcaa
     1261 acgaacagcg aattttgttc cttttcctag ttcgctttct acttctaatt ttcctttatg
     1321 caagcgtaca agttgctgca cgattggtaa acctaatcct gattcaccat attttgtatt
     1381 ttttcttgaa ggatctactt tataataacg atcccagata cttttcattt gttgttcaga
     1441 cattccaatt cccgtatctt caatcgtgat gatcgtttca agatagcctt tttctaaagt
     1501 aatcgtgatt tggccgtttt cagtaaactg tatcgcgttt tgaataatat tgaccatgat
     1561 ttggacaaat cggtcataat cagcgtaaac atcaatatca tcaacggctt tcaatgtcaa
     1621 tgtatctcct gctgcttctg ctttagcttc taactgtgca acaatatttt tcaatgtttc
     1681 tgttccattg aattttttaa tgaccatact gatttgattc gtacggattt tttcataatc
     1741 caagttttca ttcaccaaac ggatcagacg ctctgtttca tttttcatca atttaatcgc
     1801 gttttctcgc tgattttcag ggattgcatt gtattctaac ccttccagaa gtccgttgat
     1861 tgtcgtaaga ggtgttctca tctcgtgaga agcatctgcc ataaattgtc ttcgacgatt
     1921 ttcttgttct tcaatttctg cttgagattc tttcaatgaa ttcgtcattt tattgaaatc
     1981 atctgctaac tcatcaaatt catctttgtc atgtacgggg agctgtacat caaaattacc
     2041 attcgtcact tctttcgtcg cattccttaa ccgattgata cgcttaactt ggaatgctgc
     2101 aaaagcatag ctggcaataa ctgcaacgat actggaaaaa ataaatcctt tgaacaggtt
     2161 cagtgtaatt ggacgaacgc tgttatcgat atttcgcgca ggttgactaa ctacaagtgc
     2221 tccatagaat tcatgattca aattaaatgg cacaagtgcg taagaggtcg cctgtgcttg
     2281 tcctaaaata tttttattcg atgtgaattt ttcttgacgt ccattcctca gatttttcca
     2341 ttgatcagct gttaaggtaa aattcagtaa agctgtatta gtcggataaa tgacacgttc
     2401 gtttttatcg ataaatataa agttcacatt ttgttctgtc agtacttgct ctgtcagcag
     2461 caatgcattt tgaaacgcct gatcttgact aaactgcggg agcgtatcgg cataggtttg
     2521 cgttgtcttt tcaactgatt cagcatatcc aaacagttgc tggtaattgt tttcttcaat
     2581 cgtttgtttt gtcagttgtg taaaagaaac tccaacgatc aataagatca acgcgaccat
     2641 tccgcaaaaa gctaagagtt gctgatacaa ataacgcatt aggcaacacc agaatcatcg
     2701 aatttgtagc cgactcccca aacagtttgg atgacttgtg gtccgacctt ttcgattttt
     2761 tgtcgtaatt tcttgatatg ggcgtccact gtccgttcat caccaaagta ctgataatcc
     2821 cagactaatt caagcaactg ctcccgagag aatacttgac gtggtttttt tgctaaagta
     2881 aacaatagat cgaactcttt aggcgtcagt ccttcaattg gccggttgtc cagatacgct
     2941 tctcgtgttt tcgtattcat cttgaaatga ctagtctcga tatcaaaatg atcatctgaa
     3001 actgctgctt cttctcttgc ttctgccaac tcactacggc gatgcaaagc tttcattcga
     3061 gcaataagcg tcagaggact gaaaggtttt gtgacataat catctgcccc catctctaag
     3121 ccgatcacct gatcgctttc agaatctcta gctgtcagca tgataatcgg cacagtactt
     3181 gaaactttgc ggatctctct tcctactagc ataccgtcca gtgtgggcaa attcaggtct
     3241 agtgtaatca tatcccaatg ttgtgggttt tctaaaaagg catcgagccc ttcttttcca
     3301 tcatacttga aagttgcttc ccaaccttca tttaagaaaa acatctgcat catttcagaa
     3361 acagattgat tatcttcaat cattaaaata ttcattatcg ttccctccag tttccaggtc
     3421 atatttctgt aatttctttt ctgtcataat aaaaataggc ataattggaa atactagcag
     3481 aagcatttca atcagaaaat aattcgtatg cccagatgtc tttaaataat agccgatcaa
     3541 tgccatcagt aagcccataa gaaaaaacca gtttccgctt gtcttttgag ccagccgcca
     3601 atgagcgtct gtttttgcgg caagaggcga atgataccca taaaaaggca gatttccttt
     3661 tgagggcaga atccgaaaaa gactgccaat tattaagaat aagataccaa tactgaaaaa
     3721 aatcattctt tccctcctaa attctcttct tatagtatac tctaaattgt tgactgaacc
     3781 aatatgttca ggaattcttc acgaaaggtt tgtcttttgt tcattctttg ttaagataaa
     3841 cttattccaa taaaaaagga gttttatttg tgtcaaatta tcgtgcaatc actttctttg
     3901 atcttgatgg aacattacta gatgcgaaat caaaaatcac acccgaagtg gcaaaagcta
     3961 tgaacgcatt aaaagaaaac aatgttctcc ccgtcatcgc tacaggacgt acagaagcag
     4021 aaatccactc tatcatggaa gatgcgggca ttacttctgc ggtcaccatg aatggttctt
     4081 tcattcgagt agacggcgaa gaaatctatt ctcaaacatt ttcttcagaa gaatgtcaaa
     4141 aaatgtatga tcatgtacag ttgaacggtc atgaactttc ttactataac gatcagaaaa
     4201 tctggtgtac cggtcatagc caaacaatga tcaatgctta cgacttcatt cattcagatg
     4261 ttccggaaat tgatccttta ggctttaaag aaaacacagt aaatatgttg ctgatcttat
     4321 ctcaatcagg tgacgaatac tatcacgaac attttcctga gctaactttt tatcggaatg
     4381 gtccttactc gatcgataca gtgaagaaag gaatctcaaa aggggcaggt gtcaaacgtc
     4441 tgcaaaaaga acttcagctg gaaaaagttc ctacttttgg atttggcgat ggtccgaatg
     4501 attttgcctt gttggaagct tgcgacaaca aaatcgctat gggcaacgcc tatgacggat
     4561 taaaagaact ttctaccttt atcacaaaga aaaatacaga aggcggaatt gtccacgcat
     4621 tgaagtattt tgatctgatt taagatcttt ttttgaagct acctcttttc ttaagaaaat
     4681 cgcttatttt ttcttaagaa aggggcagtt attatataaa taatgggata tgattcgctt
     4741 ttttattgaa tttatcccaa tatatgctat actaattttg tatgaaaaat acacgtaacc
     4801 cttgttagct aacggggacg ttacggattc gacaggcaca gtcgagcttg aattgcgttt
     4861 cgtaggttac gtctacgtaa aaacgttaca gttaaatata actgctaaaa acgaaaacaa
     4921 ctcttacgct ttagctgcct aaaaacagtt agcgtagatc ctctcggcat cgcccatgtg
     4981 ctcgagtaag ggtcctaact ttagtgggat acgtttcaac tttccgtctg taagttgaaa
     5041 aagagaacat cagactagcg atacagaatg cctgtcactc ggcaagctgt aaagtgaatc
     5101 cttaaatgag ttgactatga acgtagattt ttaagtggcg atgtgtttgg acgcgggttc
     5161 gactcccgcc gtctccatat atgtatggtt gagacagata aacatgtatg aaaaacgttg
     5221 ttaaatcaac gtttttttat tttggtcaag gagagaaaag tagagaaata caaaattttt
     5281 tgcattgatt ttgcattcaa aaaaacattc aaacatttag aaatccccaa tacgtattca
     5341 ggaaaatcta ttttaaggtt tacaaaaaga acttttgttc gtacactctt tttgaggtga
     5401 acttttatgc tgatggaa
//
LOCUS       DAZULY010000179         5245 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000179 DAZULY010000000
VERSION     DAZULY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.181"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..1240
                     /locus_tag="ACG81A_002229"
     CDS             20..1240
                     /locus_tag="ACG81A_002229"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5015556.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            1260..1754
                     /locus_tag="ACG81A_002230"
     CDS             1260..1754
                     /locus_tag="ACG81A_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS5015557.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            1732..2418
                     /locus_tag="ACG81A_002231"
     CDS             1732..2418
                     /locus_tag="ACG81A_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015558.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(2569..5178)
                     /gene="clpB"
                     /locus_tag="ACG81A_002232"
     CDS             complement(2569..5178)
                     /gene="clpB"
                     /locus_tag="ACG81A_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFS5015559.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
BASE COUNT         1457 a         1042 c         1002 g         1744 t
ORIGIN      
        1 aataaatgga gatgaaaaaa tggatcaaaa agattttaga gaaaatcttg aattaaaacc
       61 ggtcactcaa gaacatatcg atcaattcaa tgaattgctt tcctatgtgt tccaagtgac
      121 tgaagcagac atcgaggaaa gcgggtttga gaataaacga gcttttatcc gttccaaaaa
      181 accgattttg gaagtatcga aagtgttcgg atggttccac gaaaataaac tgatctctca
      241 aatcgctatc tatccttgtg aagtcaatat tcacggtgca cgttataaaa tgggtggtgt
      301 gactggagtt ggaacttatc cagaatatgc taaccatgga ttgatgcaag atctaattat
      361 cgttgcattg gataatatgc gcaagaataa gcaatggatc tcttatttgt atccttacag
      421 tatcccctac tatcggcgta aaggatggga aatcatgtct gataaacttt cctttaaaat
      481 cagagataca cagctaccta aacaaatccc agtaagtggt atggttgagc gattaccagt
      541 tgatcatcca gacgtatttt ccgtttatga caaatttgcc caacaaaacc atggtgcttt
      601 attccgcagt gattttcatt gggaagagta ttggcgtttc gaaaatgaag atgagcgcac
      661 agccgctgtc tactacgata gcaaccatga accaagaggt gttctgttct actgggtagc
      721 tgaagaaata tttcacgtaa aagaaatgtt ctatcttgat caagaagctc gaaatggctt
      781 atggaacttt atttccgcac atttttccat gatctactgg gtacatggag atatttataa
      841 aaatgagcct ttagcatttt tgattgaaga tagtcagatc aaagagcaga tcgaaccata
      901 ttttatggct cggatcgttg atgtaaaaga attccttcaa cggtttccgt ttgttggaac
      961 agctgatgct tttcatttca tcattgaaga tcctgtcgct ccttggaata atggtgtctt
     1021 cgctttaaca tgggatgaac agggacaagt ccgtgtctta aatgaaccta taggaaaatc
     1081 cgttcgactg gatatccaaa cattgacttg cttgatgatg aattatcgcc gtgcttctta
     1141 tttggcaaga atcgaacggt tagaaacaga tgaagaaaca ctgaagtcac tagagcgtat
     1201 cattccaaac atggaagctt actttagcga ttatttttga taaaaaggat gatgtttaga
     1261 tgaaaattta ttttgccgca ccaatgtttg ctaaaagtga tttgttatat aaccaacagt
     1321 tagttaaaga aatcagagag ctctcttctg agctttcgat ctatcttcct caagaaaatg
     1381 aagcaatcaa tgacaaaact gcttatgcag acagtcgaat gattgctctt gctgacacag
     1441 aaaaagtgtt ggctagtgat ttaatgattg ctttactaga tgggctgacg atcgatgccg
     1501 gggtagcatc ggaaatcggt gtggcttatg cgaaaggcat cccagtgatc ggcttatata
     1561 ctgatactcg acagcaaggc ggaacccacc cgaaaaaaat cgctgcacta caagaaacag
     1621 ctgaaaatca atttcactac ctgaacttat atacaatagg tttgatcaaa ttgaacggaa
     1681 aagtggtttc ttcagaaatt gagttgcttt cagaagtaaa aagattttta gatggaggga
     1741 ctttcagtga ttaaagaaat caaaaaggtg caaatagctt tacttgcttt tggtgtattc
     1801 gttgtctgct ataattttta tgaatttatc acacaaaaat attcgacttc tcaaggaatc
     1861 acatttatcg tggaatcttt attagggatt gcattgattt tcatgccaca agtcattttg
     1921 aaagttttca aacttaaaat accagcagca atcgttttgt tttactggtt tttcttgttc
     1981 atctctgtct ttttaggcac agggatgcat ttgatcagta tcatctcttt ttgggacaaa
     2041 atcttacatg cggtgagtcc aatggtcctg acagctcttg gctatggatt gattggttac
     2101 ttgatgaaag atgctgagat atcaaaaacc agtccttggt tgttcctctt attcggcttt
     2161 gcgtttgctg gactttgcgg cgtgttttgg gagttctggg aattcttatg cgaccagttc
     2221 ttaggcatga atcttcaacg ttttgctgct tctgatggca cactgtttgt cggacgagca
     2281 gcattgatgg atacgatggg cgacttgctg acaaatacga taggtgctgc cttaatggga
     2341 ttgtttgcat ggtctcaaag taaaaaagat gagcgttact ttgaaagtta taaattagaa
     2401 aaagtgaaaa atacctaata aaagaggttg tgacagaagc gggcagcttc aagaaatttc
     2461 ggcttatttt tcgaagaaac tacttctgga acactgttta tttgaaaaaa ggaggtctga
     2521 cactagttgt gtcagacccc ttttttcttt tatagtctca atggaagatt agacgatttc
     2581 ttctagctct tctgttttga aatgaagttg tccatcaagt aaagtaatcg ttacttttga
     2641 tttaggcata acatgtcctg caacgatttc tttagctagt ggtgtttcta cttccttagt
     2701 gataaatcgt ttcaatggtc ttgcaccata tgctggttca taagcatttt cagcaatcca
     2761 cgttttggct tcatcactga ttgtcagcag gatttcttga tgttccagac gttgtgccaa
     2821 ttgcgccacc attttgtcaa cgattccttt cacgttgtct aggctcaatg gtgtaaataa
     2881 aatcgtgtcg tcgattcggt tcaagaattc tggtttgaag tttccacgta atagcgtgtt
     2941 gacttgttca gccactgctt ctggaatcgt tccatcagca gtcacgcctt ccagcagtaa
     3001 ctgtgaaccg atattgctgg tcatgatcag aaccgtattt ttgaaatcta ctactcgccc
     3061 ttttgaatca gtcaagcgtc cgtcatctag gacttgtaac aagatattga agacatcggg
     3121 atgcgctttt tcgatttcat ccaacaagac gattgtataa ggatttcgtc tgacagcttc
     3181 cgttaattga ccaccttctt cgtaacctac atagcctgga ggagctccga ctagacgaga
     3241 cacagcatgt ttttccatat attcactcat atcgatccgt accatatgat cttctgagtc
     3301 aaacaagttc tcagctaacg ctttcgctaa ttcggttttt ccgacaccag ttggtcctag
     3361 gaaaaggaaa gaacctagag gacgatttgg gtcttgcaat ccagcccgtg aacgaattac
     3421 cgcgtcactg accgcatcaa ctgcttcatc ttgaccaatc actcgtttat gaagtgtttc
     3481 attcaattta attagttttt ctcgttcacc ttctacaagt ttcgttacgg ggatacccgt
     3541 taatctgcca acgacttgag caatctcatt ttcggtaacg gactcttgaa ccatcttaat
     3601 ctcgctgtct tttgctttag cttctaattc cttcaattct ttttctaatt gaggtatcgt
     3661 accatgacgc aatacagcag cgcgttctaa atcatagtta ttttctgcat cttctaattc
     3721 gtgttttgct ttatcgattt cagcacgttt tgctgaaaca gaattgactt cttctttttc
     3781 tgtttcccac tgcatcttca tcgcatttgc ttcttcacga agttcagcta attcttcttg
     3841 caaatttttc aagcgttttt tacttgcatc atctgattct tttttcagtg cagcttcttc
     3901 gatttccaac tgcatcaaac gtctagtaac ttgatctaat tccgttggca tagagttcat
     3961 ttctacccga atcgttgcac tggcttcatc gatcaaatcg attgctttgt ccggtaagaa
     4021 ccgatcagta atataacggt cagataaagt agctgctgct actaatgcgt tgtcatggat
     4081 gttaacacca tggtggatct caaaacgttc ttttaatcca cgcagaatac tgatcgtatc
     4141 ttccactgtt ggttctttga ctaatacctt ttgaaagcga cgttccaacg ctttgtcttt
     4201 ttccatatat tgacggtact catctaaagt tgtggcaccg atcaaatgta attcaccgcg
     4261 agcaagcatt ggtttcaaca gatttccggc atccatactt ccttctgttt ttcctgcccc
     4321 tacgatattg tggatttcat cgatgaacaa gaggatacgt ccatcacttt ttttgacttc
     4381 tttcaagact gcttttagtc tttcttcaaa ttcaccacgg aattttgctc cagcgatcaa
     4441 tgcacccata tctaatgaaa aaatcgtttt atcttttaaa ttttcaggca catctttgcg
     4501 tacgatacgt tgagcaagcc cttcaacgat tgccgttttc cctacaccgg gttctcctat
     4561 cagaacagga ttattttttg tttttcgtga aagaatacgg atgacatcac ggatttcttc
     4621 atcacgaccg atgattggat ccatttttcc gcttttgact tgctgtacta aatcaacgcc
     4681 gtatttttct aatgctttgt attgttcttc ttggttttgt gaggtcactc tttctcctcc
     4741 tctcatctct tcgatgtttt tacgcaattc tttttcagat aatccgtttt tattcaaata
     4801 aacagttaac ggataatttt ttaatttcat caacgctaaa ataacgactt ccgttgaaag
     4861 aaactcgtcg ccgaagcttt cacgtatttg gtctgcttca ttcaataatc gaaagaaatt
     4921 ctggctgagg ttttgtccat attggacgtt tccaccagaa acaactggat actcatccag
     4981 tagacgatca atttcatgtt caaaagattc gacatccaat ccggcatccg tatagaaatt
     5041 tcgaccaaaa tgattagatt gtaaaaagat tttccataca tgggcaatgt cgatatcttg
     5101 atgttttcga gtgaccgcaa tctgttgagc ttcagcaatc gcttcttgaa gcgtcgtagt
     5161 cattttctca atattcatac aggtataccc tcctaattaa aggtaagatc acttatttct
     5221 ttgatcttta caaaaactat tatat
//
LOCUS       DAZULY010000180         5220 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000180 DAZULY010000000
VERSION     DAZULY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.182"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1256)
                     /locus_tag="ACG81A_002233"
     CDS             complement(<1..1256)
                     /locus_tag="ACG81A_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS5015560.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1270..2487)
                     /locus_tag="ACG81A_002234"
     CDS             complement(1270..2487)
                     /locus_tag="ACG81A_002234"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS5015561.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2477..4072)
                     /locus_tag="ACG81A_002235"
     CDS             complement(2477..4072)
                     /locus_tag="ACG81A_002235"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFS5015562.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            4197..4982
                     /locus_tag="ACG81A_002236"
     CDS             4197..4982
                     /locus_tag="ACG81A_002236"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HFS5015563.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            4975..5217
                     /locus_tag="ACG81A_002237"
     CDS             4975..5217
                     /locus_tag="ACG81A_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015564.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT         1574 a         1051 c          862 g         1733 t
ORIGIN      
        1 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
       61 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
      121 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
      181 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
      241 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
      541 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
      601 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
      721 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
      781 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
      841 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     1081 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     1141 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     1201 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     1261 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     1321 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     1381 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     1441 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     1501 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     1561 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     1621 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     1681 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     1741 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     1801 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     1861 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     1921 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     1981 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     2041 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     2101 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     2161 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     2221 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     2281 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     2341 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     2401 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     2461 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     2521 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     2581 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     2641 cttcttcttc gaaagtatcg acatagcgtg ggatatttaa gttatagtca ttttctttga
     2701 tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt
     2761 cgaggatttt ttgaatgttt tcttccgata atttattctg atttttccct ttaacaaatt
     2821 cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc aatacaatca
     2881 ccgtcgttgg aatcgatgta ccaaagaaca agttcgcagg cataccgatc acagcataaa
     2941 tactgccgtc ttctaataat ttttgacgaa tgaccccttc tgcagcacca cggaataaaa
     3001 ccccgtgtgg caagacgata gccatagtcc ctgtttcctt caaatgatag aaaccatgaa
     3061 gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccgtagcga ttaaaccgag
     3121 aatcatctaa gaaggtcgta tctgctgacc aattagccga ataaggtgga ttcatcacta
     3181 ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc
     3241 ggagattcat ttcttcggca tctaccccat gtaaaatcaa gttcattttt gctagattgt
     3301 atgtcgttgt atttagttct tgcccatgat atttaacgtt atctggatga gttaagtagt
     3361 tacgtacatt taacatcaag gaacctgaac ccatggttgg gtcaaagaca ctaaagaatc
     3421 gtcgttcttt ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg
     3481 tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat
     3541 acgcatcccc gatcacatcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttga
     3601 taacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga
     3661 gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg
     3721 ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga
     3781 aaagatactc tggtgagatc gaatacccca aaatatcaac gattgtggca atcaggtctt
     3841 cttttgattc ttcatctgaa agtaattcac gatacaacat tgtttgtttg cttactgtgt
     3901 catattcctc taaggattca tctgctaaaa gaaccacttg ttccaacaaa cggtcggata
     3961 aatatttata gaaaatcagt cccaataaat aatttttata ttctgacgca tccattttac
     4021 tacggagatt atctgccgca ctaaataatt tttgatttaa ttctgcaccc aaaaagatac
     4081 actcctatct cttatttaaa gttgctatat accctcttat tttagcatac tcccttttag
     4141 aaaaagcaaa aaaaaaagaa aaaatcttat tcttgataac aagctacgtg acgtatatgc
     4201 ctaacaaaaa tagaactttt gaatcaatct taaatgaagg tttaaccaca gaaccggcat
     4261 ccattatttt tgatgaagtt atcgatgacg ttgaagatag taagtatcat aaaaatttaa
     4321 aagaactttt ttattttgca ctcaacaatg gaccaagttt gttcactggt atatcagtac
     4381 ctgatagtga taatccatta attatggcaa aaatttactt aacaaaatgg tgtaataaat
     4441 acatcaggga taggaataac ccagcattaa aaaaacctct taaaacattt ggcgaaaaag
     4501 atgctgcttt aactactaga gtagcagcaa atgctaatat tgatgatcaa aaagtattga
     4561 atgactactt gcgtggacat tttttatata tgagcgctga gaacatgaac ggaagtattt
     4621 tagaggaata tttagccgaa gtgttagaac ctgaaggttg gatttggtgc gccggctctg
     4681 tatatcgagc tgttgatttc tgttatttag gaacctcacc aattctttta caagtgaaaa
     4741 ataaatacaa tacagaaagc agttcgagtt cagctataag agtaggaaca acaattagaa
     4801 aatggaatcg tttaaataag tcgacaaaaa tttctggttt ggatagccct atccccaatt
     4861 ggaaagcatt aatagaaatg actgaagcta gtaaggaact ggcagctaag ttaactgaaa
     4921 atagttattt agcttatatt aatgaaaaat ccacacgaga attatggaca ctcgatgact
     4981 aacaatatcc ttattagtat ctatccacga tacatggaaa aaattataaa caaacaaaaa
     5041 aactttgaat ttagaaattt ctatcttacc tcagatcctt ataatatctt ttggatttac
     5101 gaaacaatcc ccaccaaagc tttaaagtat aaattgattg taaaaaatcc aattactaaa
     5161 cttaagaaaa atcaacacta ctttttagga gacgatgagt ttgaacaatt agtttaaaag
//
LOCUS       DAZULY010000181         5197 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000181 DAZULY010000000
VERSION     DAZULY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.183"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..1627)
                     /locus_tag="ACG81A_002238"
                     /pseudo
     CDS             complement(1..1627)
                     /locus_tag="ACG81A_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2182..2745)
                     /locus_tag="ACG81A_002239"
     CDS             complement(2182..2745)
                     /locus_tag="ACG81A_002239"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS5015565.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(2760..3803)
                     /locus_tag="ACG81A_002240"
     CDS             complement(2760..3803)
                     /locus_tag="ACG81A_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015566.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(3936..4340)
                     /locus_tag="ACG81A_002241"
     CDS             complement(3936..4340)
                     /locus_tag="ACG81A_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFS5015567.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(4573..>5197)
                     /locus_tag="ACG81A_002242"
     CDS             complement(4573..>5197)
                     /locus_tag="ACG81A_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS5015568.1"
                     /translation="QKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFGDVDS
                     PVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFTGLFS
                     INTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTVISLL
                     IAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
BASE COUNT         1665 a         1019 c          858 g         1655 t
ORIGIN      
        1 tatttttata tgttgtaact tcctgataag aaaactaatt aaaagaaaga ggtaactaca
       61 taaactaatt aaaaaagcta acttcatagt tggaggataa atggaagaaa cttcaatgat
      121 tgtttctcct tgtgttcctt caatcgacac tagtcctatt atcaatctct cttttttgtc
      181 catttacttt tgcgactgtt ccccaataat caaatacagg aagcgttatt ttttgtcctt
      241 tagattcatg tactgtatag actattttcg aatccatcac ttgataaggc atagtatgcc
      301 attccttgcc aattcttact tcatgattct gcaaatgttc tttgtattgt aatgcttccg
      361 tgggtgcata gtcataaagt cctccgggaa ggagtgtcgt gtaacgaata agattttctt
      421 gattcattac caagtgatct ctctgagtga caactttatg ttcatagttt gcttttgttg
      481 cagcagatac agccaacagt aataaaacaa ttgccgttat attaactact tttctttgct
      541 tagctgatgt gctcttcatg aaaacgaacg ataaaatcaa cgagccaaac agtacttgaa
      601 agcctaagat tcgccaagga aattgaatca tttgaatggg tgtattttgt aataaagacc
      661 atggaaaaaa tttggtggat aagagccaag agactaccgc actaaggtaa atcacttttt
      721 ctttgcgatt aaatccttta aaaaagaaga gtggacagat caaagaaata ataccaatga
      781 ttcctaccgt aaaggttgtc aggtcattat tcatcgcacc aatcaaacta tccgagacat
      841 ttaatgcttg gtgttgtaag gtcgttttat ctggctcatt aatcgattga accatgattt
      901 gtcggaacat cggataccaa aagtatgctg ttaaaagaat cgtcattcct actgcctttg
      961 acaactccac aatcaaccgg atgctttctt tttttcttgt ttcacttact acaaagccgc
     1021 ctaagactag taagccaacg gctattacac tcataaaagt ggaaagaaca tgtgtataaa
     1081 tgagtaagct catactgact gctaatggaa ttgcacatgg ctttttctca taaatcagtg
     1141 cgtaagcata atacataact atcggtataa ccgtaagtgc aatcgcttca gcaatagcag
     1201 aacgatgata aatatcaatc gtacggtacc cataaaatgt atataaacag ctaaatataa
     1261 aagccgcatc gattcttttt aaaaatttta gtccataatg aaacatcagc atgatagtac
     1321 aaacattcaa tagccaaaca tataagatat aactaacgat taaattatta cttatccaat
     1381 aaaaaatgac tgccggaaaa aaggtcaagt agggataaaa ataattcact ccgctcccat
     1441 aatgattaaa cgtcgtaaaa ttgattggac cagaaaaaat tgaagataac cctttgactc
     1501 tagcaaaatg gaaggcaata tcccccgatg tagaaatagt atgaacattt ctaaaataat
     1561 tgattgaata aagtccagca ataattgcta ggagaatgga caatattatt tttttcttta
     1621 cttgcattac ttaattatat actcctttca tggattgctt tatctagtat aacttaaatt
     1681 ttaaataaat cgttatttat ttttactctc acttaaaaaa gcactaaaat tataagagag
     1741 ggaggtaatg gtctgaacac caagaattgt tccaacatcg aattgcttaa ctaatgagtt
     1801 caaagcttaa ttatgtggct cttatattta ccgagcgtgg attgcgatct ctttagcgaa
     1861 acaaacatct ttcataaaaa aaacagccat atccgctttc ttggatatga ctatttacta
     1921 ctactttact atctttcatt cttcttgtct cacttatgat aaggcgctgc gtatcatgag
     1981 tgaatgatat taggaaaact tcataaaata aatcttttat acatttccac ttctagatgg
     2041 tcattcatgt tagaagaatc aaactatttt tttactgtcg taataatatt aaaacactag
     2101 aacaaatcaa ttaaattcca taaaatataa ataatagacc tagcatcatc tggtataaaa
     2161 tggatgctag gtccgttata tttactcaaa attgattcta cgaatagatc ttcttatcat
     2221 ttctatttta gtagtcatgc gttgttgttc ttctaaaggt ggtaatggta taagtagttt
     2281 agataaattt gtaccattta catttggttg cccagttccg taagaaattt tttctaataa
     2341 cttccaatat aatggcgagt ctaagaagca gtctacgtat tctgataata atttctcaac
     2401 taacctaatt cgaatcaagt aagaagcaaa aacagattcc tcacttattt ccttaaccaa
     2461 gaaacttttt cccatagttc ctcctgtcct tgctatcaaa atgtcatttt cttccaatct
     2521 taaatcaact agtttagaat tactaatatc acagtaaggc acactggacc aatttacacg
     2581 tccctcttgt atatctgtga ttctcaaaaa cttaacattg ccttgttttt tcgccgaatc
     2641 tgtatagcca tattgaatct tagtagaaat acttcctaaa taaacccata gtcgaacatc
     2701 gctagatgat ttttcttgtc gttctttatg aacaacagtg atcatcttga gttgtccgac
     2761 tattttttag ttgcagcaat ctttttggga cgcttatttg catgaagcaa cgttgctaag
     2821 tctagtaata aaacattggc taacacagga ttggctttct cgcgatacgc tgcggcgcca
     2881 ctttcaaaca atactgctag acgatcttct acaacaacga tttgttccaa atacggtgga
     2941 aatttgattc gttttaggat ccggctagga gagtcccgct cggacactaa gagttccgag
     3001 gtataaggac cataagaacg actggctaag agataatggc cataaaaagc cacaccttga
     3061 atcttatcag atgtttttct gctcattgtc acggttggtt ggattttgtc tttgcggcga
     3121 attgctgttt tcatttcagc tatcgtcggc aatagataga aagtcaactg tccttcacct
     3181 tttagtgtga agttgccaga taccagttga tcattgtttt gagtcaaata cgatgcttgt
     3241 ggaatctctg agagatcagt tgtcagccaa taagcgattg gctgtcccgt ctcttcagat
     3301 gtatctgcta agatatcgat caaacgtaaa gcggctactc gtccagtagc agatcctgtg
     3361 attgtcaacc ataaaagttc ccgttttgta tcatatacta agccccctac atgtggccgt
     3421 ttaggtaaaa tcagcagttt gatcggctgt cctgtttctc tatccagtac atgaataata
     3481 gaatgatgct gatggtcata gcagtaggca gaaataaaca caaaatcttg agaaaaagcc
     3541 aacccttgcg gcgtcatcca ttgacaaacc tcattttcgc cggtttccgg caccaccatt
     3601 cttgtttctg ttaatccagg aatgttatat tgttttgctt cagccttttc ccaagctgct
     3661 tgaacaatag gataccgttt taactttttt tcaatatcct gatgtatccg ttgctgcttc
     3721 caatgcttaa actttaggac atagtaaaca agaaggccaa tgaccagcaa cactaaaatc
     3781 attattgcta cccgctgtgt catttttttg cctccttgtt ctattcgatc aatcgtccat
     3841 ataccgctat taattaccgt aactacatgt ttcttttact agcgtttctt tataggatct
     3901 ttcataatct catttatagt aacattaaga atcccttatt ctactatagt cacttccaca
     3961 tcaatattgc ctcttgttgc tttagagtat gggcaaaatg cgtgggcttc ttctgcgatt
     4021 ttctctgctt ggtccttctc aagtcctggc aaagaaactt ctaaaacgac cccgatttgg
     4081 tagcccccat cttctaattg aaataaagat acccgtgctt tgacttcaga atcagactca
     4141 agattatttt cttccagcat gtgttgaacc gcgccgttga agcaagatgc gtatccagcc
     4201 gcaaaaagtt gttccggatt tgtcccttca ctatggattc ctgggcggtc gatcttcatg
     4261 tgcatacttc cgtttggtgc ctcaacttcg ccatcacggc ctccacgatt gatcattgct
     4321 gtttcgtaca tttttttcat tgagatcatc ctttctttcg tcgttctata ttaagtgtaa
     4381 cgagtatttt ttaatctgac aaaggtaatg cttgcattcg gcgacatcag ttagacaaca
     4441 tcgatgatca gtgatagaag cagtttatct atacaaaaaa agaagggttc tatgatattt
     4501 ggtgtcctta ctcgccttga gtaacaacac caaatatcat agaaccactt aatcagcttc
     4561 acactgcttt ttctactttc gtagttgaaa aaaacttaaa caaatcatcg gcaaatagtt
     4621 aaagaaaaaa cttaatcgtt gaaagaagcc caattgctta aagaaggcaa actgactaat
     4681 cgacggaatc cgtgctaatc cgtaaagacc tgcaattagt aaactaataa ctgttaaaac
     4741 aagataaaaa tggctaagcg taccttgacc actttgacga taaagcagaa caagtaacaa
     4801 tgggaaaagt agaaagcctg catagcctag cccagaacct atattatgga tccaagtcga
     4861 tagattacaa gagctttcat tcgtattgat actgaataag ccagtaaaaa tacaatcacc
     4921 tatcccatat aaaccaactg ttgcaacaac aagtattgca agtgtttttg atgttccaac
     4981 aaataggtga tacaacgcag gcaacgaaag aacaaaaaaa agtccagata caactgacca
     5041 gaccaagaaa gcacgtctga ctggactatc tacgtcacca aacacactaa ttacggtagt
     5101 catttgattc agtttgggat aaaaaaggcc aaggatatac ggtgtcgata aatcactcat
     5161 cacacctaat aataaaaaat aaaaaccata tttttgc
//
LOCUS       DAZULY010000182         5182 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000182 DAZULY010000000
VERSION     DAZULY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.184"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..152
                     /locus_tag="ACG81A_002243"
     CDS             <1..152
                     /locus_tag="ACG81A_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5015569.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="ACG81A_002244"
     CDS             complement(230..763)
                     /locus_tag="ACG81A_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFS5015570.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ACG81A_002245"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ACG81A_002245"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFS5015571.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ACG81A_002246"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ACG81A_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFS5015572.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="ACG81A_002247"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="ACG81A_002247"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS5015573.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="ACG81A_002248"
     CDS             complement(4488..5066)
                     /locus_tag="ACG81A_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFS5015574.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
BASE COUNT         1502 a         1054 c          929 g         1697 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaaag gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca ga
//
LOCUS       DAZULY010000183         5176 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000183 DAZULY010000000
VERSION     DAZULY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.185"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..672)
                     /locus_tag="ACG81A_002249"
     CDS             complement(<1..672)
                     /locus_tag="ACG81A_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5015575.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTI"
     gene            complement(683..1351)
                     /locus_tag="ACG81A_002250"
     CDS             complement(683..1351)
                     /locus_tag="ACG81A_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFS5015576.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1481..2065)
                     /locus_tag="ACG81A_002251"
     CDS             complement(1481..2065)
                     /locus_tag="ACG81A_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5015577.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2396..2629)
                     /locus_tag="ACG81A_002252"
     CDS             complement(2396..2629)
                     /locus_tag="ACG81A_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFS5015578.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(2644..3594)
                     /locus_tag="ACG81A_002253"
     CDS             complement(2644..3594)
                     /locus_tag="ACG81A_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFS5015579.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3591..4433)
                     /locus_tag="ACG81A_002254"
     CDS             complement(3591..4433)
                     /locus_tag="ACG81A_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFS5015580.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(4435..5154)
                     /locus_tag="ACG81A_002255"
     CDS             complement(4435..5154)
                     /locus_tag="ACG81A_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFS5015581.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
BASE COUNT         1765 a          932 c          639 g         1840 t
ORIGIN      
        1 aatagttgta gtttttacag ttctattttt aacaaataga acttgatcaa ttaattcttt
       61 caaatagtaa aaacgatgtt cagctataac aatagtaaat cctttatttt ttaaatcaca
      121 aagtaagtca gcaagtttct tagtcgcttt gaagtccaaa tttgaagacg gttcatccaa
      181 tataattaat ttagagttta gcgataaact acatccaata gaaattttct gccgttcacc
      241 acttgatagc gaaaaaatgt ttttatctaa tatatttgaa agatctaata attcagctaa
      301 ttcattgatt cttttatcca tatgctcttt tgtaaatcca aagttttcca tcgaaaatac
      361 taactcactc gttgtatttg ttgtaaagaa ttgtcctcta ggatcttgac ttacgtagcc
      421 aatatttttt gatatacttt cttgtgttaa ggattgtagc gagttatttt caaataaaat
      481 ttccccaacc aattttccgt cataaagtct tggaataagt ccattcatta actttaatag
      541 tgtggatttc ccactcccag aagaaccagt aatcaatgta agactgcctt tttttattga
      601 aaaattaagg tcaacaagcg cttgttcctt aacatcctta tatgaaaaag aaacattgtt
      661 aaattcaaac aattctttca cctcaaatca aaatacttaa gaaaaaaata gtaacggaaa
      721 acaggatgat atagtctaat aagctcatgt ttgttttata gtaagaactt cttttcacag
      781 gagattctat cccctttgtt attgcattag cggtcatttc ttcagcaatc atagttgcac
      841 gtataagaac gggaactaac agaaattcca aatattgcag aggctgaaaa aaagacaagc
      901 gaatgtttct catttttaaa gattctttaa tcattcttat ttcagaacga taagtcggaa
      961 agaatctgat cactactgta atacttaaca ttattttttt cggtaaatgt attttttcta
     1021 aagaggataa taattcattg ctagtataga cactaaccaa caaccatgag atcattacga
     1081 ccggcaacat tcgaaataca atatataagg atgtcgcaaa aaatatagta aaaaactcta
     1141 tcttgatagc atcaataatt ttcattacta aaaacagaac tgaataaatc aagatgaaat
     1201 aaatacatgt ctttatttta cgaaacatca atactaaaac tgtagcaata atgaaacacc
     1261 ccacttgaaa tatatttccc tttcctaaaa aaataatgac cggaaaaaaa atatttaaag
     1321 taatcaaggt taacggattg atttttgaca ctctttcacc tccaaaaaat aagatcgcac
     1381 tgttatctta agctgataaa aatgcaccgt tattcagata acagtgcgat cttatcaaaa
     1441 agcataactg agaacaattc tcaatgcaag ctttttttaa ttaactcagt aattcaatcc
     1501 acccaggaac agaaaattga tacaatcttc tggttaatag catagtcttt tcttttgaat
     1561 agctatgctt caatatatca tagaatgtag cgtagaatga gtaaatgtag aagtggattt
     1621 cttcttcatt aaagactaaa ttttgattga tcattccgat agtctttaac ttatctagac
     1681 aacagactgt ctttttcgtc acaaatgatg taattttttc aacaatatct tgatatttgg
     1741 ttccagctga ttggaataaa atcaaatcga atatacgttt attctcaaaa atataattaa
     1801 taaatggatc taattcattg aagttctctc tccaactttt ctctatatct atattttcta
     1861 agtttaagag gtatttggta aactcttcat tatacatatt aataagcgca ttaatatgcg
     1921 gctgaataat ttcttcaaat aacgcttcct tagaagtaaa atgtttataa aaagcacccg
     1981 tagttacgcc tgcggtcagg cacagttgcc ttatcttagt ttgctgaaat ccttcttttt
     2041 caaaatggct tagtccactt tctattaact tttggtgagt tataatataa tctctggttt
     2101 ctatgggtac ttcctcctaa taagatgaat taactttatt atcttacaca ggacttgaag
     2161 tttcaatcgt taacgtttat tactgaactc attccttaga gctaaactaa tgagaaagag
     2221 cagaataact gatacaaaaa gcaatcctac aagaattaat agactcatct aaatcattca
     2281 ccttttctaa aataatccga gaaagattta aaaaaattat atccagctat taggataagt
     2341 cctatcataa tcactccaat agccattttc ttatcctccc taaaaataaa gtcttttact
     2401 taaaaccttt tttgtaatcg tcaataaatt ttttcccttt atcagttaat tgaaatccta
     2461 tatctaaata gtcctcatat ctaagtttag ccagcatctc acttacagat gctggactaa
     2521 cacttagaac ttttgctaaa tcattatttt ttaatttctt ttttcttttt gataattcct
     2581 gaattgctag taaataaatt agtatagtag tcgttttaat ttgtaccata cctaccctcc
     2641 aaattatttc gaaaggcctt cagcaatttt atccaggttc catttcatca tcgcatagta
     2701 actatcacca tcttgtcctt tttctgcaat tgaatcagta aaaatcgttg aatagatagg
     2761 aatattggta tcttttgata ctgttttcat tggtctatca tccacactgc tttctacgaa
     2821 taaggaggga acttttgttg tgcgtaattt ttcaactaag tgttttattt gatctggtgt
     2881 tccttcttct tcagtgttga tttcccaaat gtaagcagaa ggcacattat acgctttcga
     2941 gaaatattta aagcatcctt cacttgtaac aatcattttt ttatcattcg gaattgaagc
     3001 aaatttagat ttagcttctt tgtctagaga atccaatttt tcaatatact tatctagatt
     3061 ttctttatag aattttttat tatcaggatc tttttccgct aactgttttt caatattttt
     3121 agcgtaaata ataccgtttt ctaaatttaa ccaagcatga ggatcttcct tccctttctc
     3181 actctgaccc tctaagtaaa taacatctat gccatcactt gctgcaaaat agtctttgtt
     3241 ttcctctttg ttcgcatttt taactaattt tgtaaaccaa gcatttcctc cagtttccaa
     3301 gttaacaccg ttataaaaaa tcaaatctgc ttttgaagtt ttttgaacat cttcaggcaa
     3361 aggttcatat tcgtggggat ctttcccaat aggtacgata ctgtgtaaat caattttatc
     3421 ttttgctata ttttcggtaa tatctgctaa aatcgaatta gtaactacta cttttaattt
     3481 ttcgttcgaa gccactttat ctttgtcttt actatttatt gaacatgccc cgaatataaa
     3541 aagagcaatc aaaatcccca gacttcccag tactgctttc cattttctca tcataacgat
     3601 ctctccttct ttcttaaaaa tttttgtttt ggtgagatta gaaaactaat tacaaataag
     3661 aatgccgctg ttaaaacaat acttgatcct gctgctatgt taaaactata accaataaac
     3721 aatccaagca atgatgaact tgctccgact gttgcagaaa taaatatcat agttttcaag
     3781 ctatttgcat ataaatatgc tgtagctgct ggtgtaatta acattgcaac aattaatata
     3841 gttcccacac tttgcatagc tgttactgct actaaagtta atagaaccat caatagataa
     3901 tgataaaaac tgacattcat ccctattgct tttgccatta atggatcaaa agaagtgatt
     3961 aataattctt taaaaaatag aactactata accaacacta aaaccgaaac gccaattgta
     4021 atccacttat caatatcttg tacagctaaa atatttccaa ataaaatatg aaataaatcc
     4081 gttgagctat ttgcaactcc tataaggata actcctagtg ctaaaaaaga actgaaagta
     4141 ataccaattg ctgtatctcc ttttattaca ctattgcttt ttataaaagt aataatgata
     4201 gaagctaata gaccaaatac aatcgcacca ataaaaaaat taatacctaa aatataagaa
     4261 attgctactc ctggcaaaac agcatgagaa atagcatcac ccataagaga cattcctctt
     4321 aaaataataa aacaacctac tgttccagaa acaatcccta taactacaga tgtaattaga
     4381 gcattctgta aaaagtgaaa gtcatacaga ccattaataa aattttgaag catattattc
     4441 cccctctcca ataaatatag tatctccata ggcttttttt aaattttctt cattaaatac
     4501 agattctaca cttccgtgag caattagatt tcgattcaaa agaataatat tatcaaaata
     4561 cttctttacc ttgcttaaat catgatgcac aattagcact gtttttccct gatgtttcaa
     4621 tttttttaac gtatccataa ttattcgttc actaactaag tctatcccta caaatggttc
     4681 atctaaaaat atgaaatccg cattttgagc aagacatctt gctaaaagta cacgttgaaa
     4741 ttgtcctcca gataattctc ctatttgatg attagaatat tttcccatat cgaccttttc
     4801 caaagccttt gaaactcttt gccattctgc atttttaatt ctttgaaaaa ctttcattcc
     4861 ggcatatgtt cccatagata cacattcttt tacttttatt ggaaaagtaa aatcaatatc
     4921 agctttttgc tctacatatg caatcttcac cagtttttta ttgatatctt cattattaat
     4981 ggagacctct ccctgatgag ggataatatt taacattgct ttaagcaatg tggattttcc
     5041 agcaccattt ggaccaataa tacctgttaa agcaccttct tgtattttta aagaaatctc
     5101 gttcaatgct aaaaaatcgt tataacaaac attaagcttt ttcacttcta acataacttt
     5161 ctccttctaa atgata
//
LOCUS       DAZULY010000184         5153 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000184 DAZULY010000000
VERSION     DAZULY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.186"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..648
                     /locus_tag="ACG81A_002256"
     CDS             <1..648
                     /locus_tag="ACG81A_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015582.1"
                     /translation="DTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLS
                     AVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMR
                     YQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITM
                     IHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            707..1147
                     /locus_tag="ACG81A_002257"
     CDS             707..1147
                     /locus_tag="ACG81A_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015583.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1159..1827
                     /locus_tag="ACG81A_002258"
     CDS             1159..1827
                     /locus_tag="ACG81A_002258"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS5015584.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1891..2514
                     /locus_tag="ACG81A_002259"
     CDS             1891..2514
                     /locus_tag="ACG81A_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFS5015585.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2570..2956
                     /locus_tag="ACG81A_002260"
     CDS             2570..2956
                     /locus_tag="ACG81A_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFS5015586.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3169..4560
                     /locus_tag="ACG81A_002261"
     CDS             3169..4560
                     /locus_tag="ACG81A_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFS5015587.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1707 a          861 c         1084 g         1501 t
ORIGIN      
        1 gatactctga ttgagcgatc tacatttcta tatgttcaat tgcaggaaaa taaaacattg
       61 ctgcattttt caccagagtt ccatctagag gggctaacat taggagaaat ctatcaagca
      121 gaagggctgt cggctgtttt gcactttttt aaggtagaat tggaattacc agtaaaagat
      181 tatatcgagc tttctcttga atcttgggat cgagcagtgg ttgaactgtt tccagaaggt
      241 ttaatgatcg atacaaacga tgggaagatc catctgactg cagcagaact gcaaaaacaa
      301 atgcgttatc aaatagatga cgaaaattct ttttctattt ttagacgtca gcaaaagatt
      361 ctaaaaagct ttttaaaagt gatcagtaaa aaacggaatc tgatcaaaac gacacagctg
      421 ttgaaaaaat atccaggtct gcttcacacg tctattcaat ttcctcaatt gattacgatg
      481 atccatcgtt ttatacgtat gcaacagcta ccatctaaga agctgtttct tccattgacg
      541 gacacatttc gagtagtcaa cagagaagat aaaaagaaag tgattgtgat tgactttacc
      601 agaaatcgga atgtccttca ccaagttgca atggaaaagg ccaattaagg ctttttcttt
      661 ttggattttt ttgtcataat aaatattaga tgatggagga gataatatga tgcaaacatg
      721 gttagaaagg ctaatacgat ttcagcagaa ctatcacaaa cttatgcagg gaagatatgc
      781 gaaatttgat cagttgaatc gcggtctatt gatcgctgcg cttgtcttta gtttgctaag
      841 tcgttggttg ccctatagaa tcggacaatt gttgttcatc gtctttttcg gatggatgat
      901 atttcgtttc ctatcaaaaa aaatctaccc cagactaaat gagaatcaac gttatttgaa
      961 atatcttgat agagtcaaag aaaaatatca tcagataaaa aacaaaaagc aagtcaaaat
     1021 ggatcgaaaa agttatactt tttttgagtg cccgaattgt catcaaaagc agcgtgcacc
     1081 taaaggaaaa ggacgaatac gtgtgacatg caaaacatgt ggtataaaat tcgaaacgaa
     1141 tgtttaaggg agaaaagaat gaaattagta ttattacgtc atggcgaaag cgaagcaaat
     1201 tttgaaaatt actggacagg atggctagat gtcgccttaa cagaaaaagg aaaagaacaa
     1261 gcagcagttg ccggtcaaaa aatgcttgaa gccggacttc aattcgatgc cgtttatacc
     1321 tctgttttga aacgtgcgat tcttaccgga caagtcgcac tagaagaaat gggtcagtta
     1381 tggcttccta taataaaaac atggcgtcta aatgaacggc attatgggga tcttgtgggt
     1441 aaaaacaaag ataagatgaa agaaatatac ggaaaagatc aagtaaaaac atggcgcaga
     1501 ggatatacgg aagttccgcc gttaaccgca gaaaatagat ttgaccgcag atatgatcac
     1561 ttggatccgc atttgatccc atacggagaa agtctggaaa tgacagtgaa tcggatcatt
     1621 cccttatggc aagatcatct agcaccaaaa ctaaaagatc ggcaagattt actagttgtg
     1681 ggtcatggta atagcatccg agcactgaca aaatatttag aagatgttcc tgaagatcaa
     1741 atggatacga tcgatatccc aaatgcacaa ccaatccagt atacattagc agatgatttg
     1801 acaattctgg acaaaaaaat actttaaaga acacctttat ctaaaagtga gagatagaaa
     1861 aaagaagcaa aggaatggaa caaaaggaac atgaaaaatt atatatggga ctttgatggt
     1921 acattgtttg atacgtatcc cgcaatggta gatggtgcat ggcaagcact aaaagatttt
     1981 ggtatcagta tggacaaaaa agaaatctac ttcaaaatga aaaagtattc tactagttat
     2041 ttgatcaatg aatcaaactt gaatgccaga gagtttaatg aattatttca ccgatatgaa
     2101 aaagaaagta cggaagtttc ccgtccattt ccagaaacga aacaagtact ggaaatgcta
     2161 aaagacaacg gcggtcgtca tttcatcctg acccaccgtt tgacagaatc aacatggggc
     2221 ctgttaaaag aacatcgatt ggcacatctg attgaggaag tagtagggat cgatcaggac
     2281 tttcctcgta aacctgatcc agcatcttta aattatttga tcgatacttt tcatttagaa
     2341 agaacagaca cgatgatgat cggcgatcgt cgtttagata tcgaagcagg gaaaaatgca
     2401 ggagtcgcta cttgtcttta cgacatcgat cattttctgg gtgagatccc ggcagattat
     2461 gtggtaggaa atctgaatga aatccttacc ttgcagagca tcattaaaaa gtagtatccc
     2521 ctttcaaagg agatatgtta taattttcat aaaggagtga taaaattata tggaaatcag
     2581 aagacaaaaa gaagttgtag aagcatttca ttatgatatg agattgccga accaagaagt
     2641 agaaacagat ttgaaagtag gcttctcgcc tttaaaagaa acgatcgaaa actatccgaa
     2701 agaacatagc atcgtaggag cacgcttaga gttccgttta gtgtttgata catatgtttt
     2761 atctggaagt gtgagtcaga tcaatcatat cctcaatcgg aaaattgaag gtcaaaatga
     2821 tgtcacacaa gaagaagtgg acgagctagt agcaccgtta ttcgatatcg tacgccgaat
     2881 gtcttatgag gtgacagaga tcgccttaga tcagcctggg gtaaaattga attttcaatc
     2941 agaaaaacaa gaatagacag taaaaacata cctgcgatag attaaagcta tcacaggtat
     3001 gtttttttat tactaattgt aagcaaaaag aatgtcattt cgataaaaat cgtctaaaat
     3061 ataaaagtca ctgaaaaaaa ttttttcagg aggatggttg aagaatcatt ctcgggaacc
     3121 tttacttcct tcgatggact tgtcattaat aatatgagga ggagaaaaat gacaacactc
     3181 atcacagttt tagtggttct agtttttgcg gcattattat atgtgttcta tcgtatgcaa
     3241 aagcgccata caaaattttc aacacgcgtc tttacagcat taggagttgg tatcgtcttt
     3301 ggcggtctgc ttcaattgat ctttggtgta gacgacaaag tcgttactca atcaatggaa
     3361 tggatcggga ttgtaggtca aggatatgta tcattcttgc aaatgctagt tatgccttta
     3421 gtgtttattt cgatcattgg tgcattcaca aaaatgaaag tttctgaaaa gctagggaaa
     3481 attagtgcga ctgtattgac ttctttactt ggaacaacag ctattgcagc attgatcgga
     3541 attggttcaa caatgctgtt cggactccaa ggagctaaat tcacacaagg agctgcagaa
     3601 acagcacgaa tctctgaact ggcaacacgt caggaaacag ttgaaaactt atcgattcca
     3661 cagcagattt tatcctttat ccctaaaaac gtttttgctg atttagcagg tagtcgtgca
     3721 acaagtacga ttggagtcgt tattttcgcg gcatttgtcg gaatcgctta tctaggcgtg
     3781 aaaagaaaag cgccaaaaga agcagcattc ttttcagatt tagtcgacag tctgtataaa
     3841 atcattatgc ggatcgtgac gcttgttttg cgtttgacac cttatggagt atttgctttg
     3901 atgacaaacg ttttggcaac aagtgatttt gcagctatcg tcaatttagg taaatttgtc
     3961 gttgcttctt acgtagcatt gcttgctgtt ttgattgtcc actcactgat tttgattggt
     4021 gcaaaagtca atccaatcaa ctatttcaaa aaagcctttc cggtattgag ctttgcgttt
     4081 acttcacgtt caagtgctgg agctttgcca ttgaatatcg agactcagac aaaagcttta
     4141 ggagtagatg atgcaacagc taatttctca gcaagttttg gtttgtctat tggacaaaac
     4201 ggctgtgctg gcgtttatcc agctatgttg gcaacgatcg tcgcaccagc agctggagtg
     4261 aatgtcttta gtttggaatt tattttgatg cttgtggcaa ttgtaacaat cagttctttt
     4321 ggtgtagctg gagtcggtgg gggcgcaaca tttgcttcac tgatcgtttt aggggcaatg
     4381 aacttgccag tagcaatcgt tgggttagtt atctctgttg aaccattaat cgatatggca
     4441 cgtacagcag taaacgtcag cggtagcatg gtagctggta tcgtgacaag tgcacgcatc
     4501 aaagatttag atcgcgatgt catcaatgat gattctaaag tgatcgatgt gaatgcatag
     4561 tcaaaaagag gttgtgatag aagtggtcag ctttaagaaa taagaagaag tttcctaaaa
     4621 atagtttctc aaatttttgt gaaatttcgg cttatttccg agaagctact tctgaaacac
     4681 cgtgaataag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaaacaaa
     4741 aatagttcct catattttac gtttggttga gcttattacc gcagatgcaa gcttttgaac
     4801 accgtttaag aaagaggtcg tgagcctgcc gtttcactct gagtcacaaa gagcaatttc
     4861 aaaaaatagc ttctcatgtt tgttgaatat tgtgtcttgt gaccgaggag ttgtcaggag
     4921 aacaccgttt accagaggtt gtgacttgtt tttagtcacg acctcttttt ttatttattc
     4981 catataaata cacattattt attccgaaaa gtgaaagcgc aaataacaaa aacaaccaac
     5041 ttatttatta ttaaagaaaa gcgtatttaa gtaggacgat tctgttgaga gctttcgtag
     5101 attttcccat tttcctatgg taaactgaca aagttgagaa tgttagaaca gat
//
LOCUS       DAZULY010000185         5136 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000185 DAZULY010000000
VERSION     DAZULY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.187"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..554
                     /locus_tag="ACG81A_002262"
     CDS             15..554
                     /locus_tag="ACG81A_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFS5015588.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(629..1138)
                     /locus_tag="ACG81A_002263"
     CDS             complement(629..1138)
                     /locus_tag="ACG81A_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFS5015589.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(1518..2279)
                     /locus_tag="ACG81A_002264"
     CDS             complement(1518..2279)
                     /locus_tag="ACG81A_002264"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFS5015590.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            complement(2276..2497)
                     /locus_tag="ACG81A_002265"
     CDS             complement(2276..2497)
                     /locus_tag="ACG81A_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFS5015591.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            complement(2516..4102)
                     /locus_tag="ACG81A_002266"
     CDS             complement(2516..4102)
                     /locus_tag="ACG81A_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5015592.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            complement(4102..4803)
                     /locus_tag="ACG81A_002267"
     CDS             complement(4102..4803)
                     /locus_tag="ACG81A_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFS5015593.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            complement(4796..>5136)
                     /locus_tag="ACG81A_002268"
     CDS             complement(4796..>5136)
                     /locus_tag="ACG81A_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFS5015594.1"
                     /translation="HLPIPGSILGILFLFLAFEMHLVDPEKIGATGDFLLNNLAILFV
                     PAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVAAGKTAEGVEALLGYVRKKKAERVS
                     VNEMEENQID"
BASE COUNT         1566 a         1073 c          943 g         1554 t
ORIGIN      
        1 gaaaagaggg cgttatgaac cgaacattga aagaattgat acttgctgcg gaatttgccg
       61 cgattatttc tgtcctttct cagatgacca ttccttttgg tttggttcct ttgacagggc
      121 aaacatttgc tattggatta acagcaactt ttctcggaag aaaactagga ttgatatctg
      181 ttggagttta tcttttactt ggcttgattg gtcttcctgt attttcaggg atgactggag
      241 gtatagctgt tttatttggg ccaactggcg gatatttgat tggatttctc ttacaaacgt
      301 ggataactgg ttggatgatc gaaagaacgg atgctcatta cttatatgca atctttgcca
      361 atctaatggg ctctcttgct gcattgatct gcggaacgat ctggctgaaa ataagtggtg
      421 atcttacttt tacaactgca tttgccagtg gactactgcc gtttttactg ccggaagcaa
      481 tcaaggcaac tggagcagct tatatcggta tcttgatgaa aaaacggcta aatcgatttc
      541 ttcatacaaa ttaatcaaaa aagcagcatg gaagaagact caacattcag tctcttccat
      601 gctgccctat tttaaattaa acagatcttt attttgacaa atccactttt ttaccgatcc
      661 aataaataat tatcccaccg acaatcattg atgccaattc tcctaaagca atcacccacc
      721 aattcaagaa aaaaggtagt tgatataaaa tcgttagctg accagctact gtaaacatgg
      781 aaaaagcaaa tacgcatgct gtcacggcca ttttgatttt catgtttttg atgcgactag
      841 tgattttata gttgataagc aatacaagca gcgtgctgac gcttcctact gcaacatcta
      901 tcagtccaag aggagaagca atattagcca aagctactcc cgctgttacc gcgacgatgt
      961 atcttttgtt atacaaggac agatagttga acatctccga gagacgaatc tgaatcactc
     1021 caaagctaaa gacagacaaa accagcgtca ctgccacata taaaccagtg actagcgcca
     1081 tccgagtcac atctgtcgtt gtccagcgtt tggctgaacg ggtattattt ttttccatgt
     1141 tctatttctc ctttgcaaga taattttatc ttggtaatga ccgcgaccaa aggatgggaa
     1201 gaaaattcca actgaagtgt cgcagaaggt agtgaaaaag ctgccctgca acaagtaata
     1261 agaatggtca aataaaaata gctccttata tttttcattt ggctggactt gttaccgcag
     1321 ttgcaagctt ttgaatatcg tttatatcaa gaggtcgtga aaagccgctt agtttcgaat
     1381 attaacgatt attaaaaaga attttttgaa ctggctgttc ggcatcgaat gtcgggaaca
     1441 gtcatcgact ttttttatca tcaaccgttg tttctctctt tagttttatc atacagaagc
     1501 ttgaaaaaaa cacaaggtta ttttaacaac catccgccgt cgattgggat gatcgttcct
     1561 tgtatgtagc ttgcttgagg agaggcaaga aataacgtca gctcagctac ttcttccggc
     1621 tgtgcccatc gcttgactgg tgtttcattt gccacccact ctgccatctc acctttccct
     1681 tcaaaatcag ccgcattcat tggtgtttgg atggctcctg gagcaattcc ttttactcgg
     1741 ataccctgtc ctgcgtaatc cagagcgagt tgtttggtaa atccgacaat cgcatgtttc
     1801 gcgcttgtat aagcgatccc acctccacca gcaatcaagc cagcaatcga agccatattg
     1861 atgatcgttc cgcttttttg gcggatcata tcaggaagga tgatttttgt cagctgatac
     1921 atgcttttca cattcgtctc ataaatcaga tcccacagcg attcgtcagt ttccatcaag
     1981 ggtttgtagg catccaatat ccccgctgta ttcagtaaaa tcgtaacttc accaaaatga
     2041 ttttggcatt tcgataccgc ttgttggagt tctttcattt gtcgaacatc acctagtgcg
     2101 taggcaaaag tttcaggaaa tcgcaaagaa atttcttcca ttttaccgaa cttttgatct
     2161 aatccaaata caaaagcatc ttgttcaaga aacgctcggg cttgcgcagc accaattcca
     2221 gaagcagctc ctgtgataaa tacaacctgt ttagctagat ctggatactg gtttttcatt
     2281 ctacgatcgc ccaatcatct gctaaaatat cacaaactgt aggagcgaag ctggaaaaac
     2341 cttcgtcact tgttttgatc aataagtacg gcgtcaccgg acagccatca aattcatcat
     2401 cggatacgag aacaacataa agttcaaatc cactccatcc tttacgaata atctttgcac
     2461 cttttttcaa ttctggtaaa atttcttcaa atgtcatctt ttttctcctt ctttgttatt
     2521 gttttaatag tttttgtttt tgaatcattt caaatttttg agatagttgg atgtaatcaa
     2581 ttaattttag ttcttcagtc acaataaaat agtcaagatc cgcccatggt ttgcggtgga
     2641 ctttacttgt cgaaatcaga agatcaactt ctctcccccg ataatctgtt ggcagaaaaa
     2701 tcagctgata ctcattacgg aaaaatgtcc gtaatccctc ctctaacagt tgctcttcaa
     2761 ataatgggaa atctgtcatt aaaaggatcg tgatctttgg aagctgtgga gtaaaggctc
     2821 ctaatgtttt aaacacagtc agataccgcc ccatcaaaaa cgcatgattt ttaaatgcca
     2881 gatgattggg atgtttgtgt ctactttcaa aaaaatgtac gagccattgt ttcaattcct
     2941 tgttttcaat aactaaatga ttaataaaat cactactatt gccatcaatc gtttctttcg
     3001 cattaggaaa aagataggcg aaaaaatgac tgctgaataa atagttttct gcctcgaagg
     3061 ttagattttc tgctggcagc ccctgctctt cctgataaag agaaagagcg gcttttgctt
     3121 ctgaaagggc tgtaaagggc ggtgtttttg cttgttgatg aaaatcaaaa attttcttcc
     3181 gtatcttagg atcactataa tattcttcac gagtcatcag tacaagaaat aatgcgccta
     3241 actcgtcttc tacttgaaaa taattaggaa ataccggctc catgacctgg caaaatcgct
     3301 ggaacaacag attgtcagaa atcagccgtc tttttttctc attcaataca acataatgct
     3361 tctgtgattt tcttaaaaaa aaggcaccta gcatatattc caatcgtctt tttttgatct
     3421 cgttcagacg aacattaaaa aaagccatga tatgttctgc catattcttt acgtccattt
     3481 gagaaatcgt ttcaaatggc cagattttcc ctcgaaacaa ctgccagaat gtcatatgca
     3541 agtaaagtcg aatgatttct tcttcgcctt gagcgatata tgtacctcgt tgcagccgaa
     3601 ttccgtaagt ttgcagttgc tgtttccatt cattgatttt tcgatgagca gtagattcac
     3661 taatgtaatg cttttgcgca aattggtaca ttgtatggaa ttcttcaaaa aaaatgtcgt
     3721 gcagcaactg gaatttcagc gtactttgca cgagatagat caaaaaacgc tcatgttcaa
     3781 ttgaatcgag cgtatcataa aagttataac cagaacggtg attttttgta aacaaagggt
     3841 gtgtcttacc gctaaactgc tgatagtttt tagacagctc gttcaggtat ttcgacacgc
     3901 tgcgggcatc cagctgaaca acttgcgcta attgtgcagc ggtatgccaa gcttgatcct
     3961 caaaactttc taggaggaga atcttataat atagatcttt atcaaattgt ttaagatact
     4021 cggaagacaa atacacgtct ttccctcctt ttttatgctc gaacgttttg gttattgtag
     4081 cataaaatag tggttttcgc atcaaaacaa gccagcaaaa ggctgaaaga ataacggtag
     4141 caataaaacg gtcaatattc cgttgacgca tatcgcaagt ccggaaatga ttccttctat
     4201 ttcaccaatc tcgattgctt ttgctgtacc aatagcatga gaagtacttc ctaacgccac
     4261 accgcgagca actggacttt cgatcttgaa taggcggaaa atatgagtgg cgatcagcga
     4321 accaaaaata cctgtagaga caacaatagc aagtgtcacg gcattcaccc cgcccatcgt
     4381 atgagataaa tcgacagaaa tagccgtcgt tacagattta ggtagtaatg agatcaccat
     4441 ctctttatga agagagaaga agtaaccgaa ccctacacta accaagcaat ttgcgactat
     4501 tcctgctaaa atcgcagcca ttacaggata aaaattctct ttcagtaaac ggaagttttt
     4561 ataaagaggg atggcaagtg ccactgtcgc aggagtaatc agcatagtca aaaaattgcc
     4621 cccgatatta tagtctttat agctgatccc agaaaaatgc aaaaataaga taataacaac
     4681 aatcgataat acgagaggat tcaaaacagg cagtttatat ttccgcaaaa atttgtcaga
     4741 taatacgtac actcctaaag tcaaacttaa gccaaataat ggattcacaa aaaactcagt
     4801 caatttgatt ctcctccatt tcgtttacag ataccctttc tgcttttttc ttacggacat
     4861 agcccagcaa agcttctaca ccttctgccg tttttcccgc tgcaaccatc gtgacgatgg
     4921 aacacacgac gactgctccc agtaaaacag gccatatcga agcaatatca tcaaaatatt
     4981 ccaataaacc tacaccagca ggaacaaata aaatggctag attattcaat aaaaaatcac
     5041 cagttgctcc gattttttca ggatcaacaa gatgcatttc aaatgctaaa aataaaaaaa
     5101 gaatacctaa gatacttcca ggaataggta aatgag
//
LOCUS       DAZULY010000186         5061 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000186 DAZULY010000000
VERSION     DAZULY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.188"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(205..660)
                     /locus_tag="ACG81A_002269"
     CDS             complement(205..660)
                     /locus_tag="ACG81A_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LMxysn_1693 family intestinal colonization
                     protein"
                     /protein_id="HFS5015595.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
     gene            complement(1063..1236)
                     /locus_tag="ACG81A_002270"
     CDS             complement(1063..1236)
                     /locus_tag="ACG81A_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015596.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYN
                     SPNEELFVKETDK"
     gene            complement(1917..2456)
                     /locus_tag="ACG81A_002271"
     CDS             complement(1917..2456)
                     /locus_tag="ACG81A_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS5015597.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            complement(2472..3395)
                     /locus_tag="ACG81A_002272"
     CDS             complement(2472..3395)
                     /locus_tag="ACG81A_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS5015598.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            complement(4523..4678)
                     /locus_tag="ACG81A_002273"
     CDS             complement(4523..4678)
                     /locus_tag="ACG81A_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015599.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRI
                     FEQRKKM"
     gene            complement(4697..>5061)
                     /locus_tag="ACG81A_002274"
     CDS             complement(4697..>5061)
                     /locus_tag="ACG81A_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFS5015600.1"
                     /translation="FSVLFLINILNTSFSEGINPNEVNGSLLLFFLNAATYGAFLEEG
                     IFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFVYIQTKNIWYSI
                     VAHGFYNTIGILIYLISI"
BASE COUNT         1607 a          839 c          771 g         1844 t
ORIGIN      
        1 aaaaaaaact atatttaaag agttttttta gatttacttc tttgaattca cgaatgaagg
       61 tagaatgaaa tagtataatt attagcacta aaaaaaatat atcactatgt tcaaataaaa
      121 aatatatatt tgacggtact cctaatgatg ggatagcaaa ggtcaaaacc aagtaagcaa
      181 tactaattat ccattttatt gtcattaggc aggaatataa tcaatatatt gtgtatttgc
      241 tgtagaagtt tgagtacgaa tatatttatt atctacctta cgataaactc tgtaggttac
      301 agttattctt gcattttgac gatatttttt aaattttcca cttgcagaag tgtttctggc
      361 tatttcgatt ttcatcccaa atacatttgc actgacagat aactttgtac ctcgactatg
      421 agtttctata cctaaataag tacggcttcc tttagcaatt gaccagcttt gaggtttaga
      481 ccaagtcctt gtttgaataa ctggttcgtc taacacatag tagtactcat catcagaata
      541 agtggctata tcagaaatgt tgttctcatt agcgaatacc gctccgctag gagtaatagc
      601 agaaccaaaa gttaaaatag aagtagataa taaaaaggtt ttaaataact gttttttcat
      661 caaaatcacc tcgaatataa gatacttaag aaaacgcttt ctttatataa ttagaataat
      721 caggaaattt aatgttgtca agagggttta ttttataaaa gctgttatat taaaggttag
      781 tagacataat gtacagtatg ccaagtagcc cttgtatcaa ccgatacagg ggctcacttt
      841 gtgattttta attggtctag gtaaaaagct ctttttaagg cttgattttc tgcagttgct
      901 tctttccgtt tctttgtgaa tttttaccag tcatttttta ctggattgtt atttttcata
      961 aaatatgata aatttttggt agcgctttat aagcgcgttg ctttttcaag aataaacact
     1021 cattattttt taactggctg ctcggaagag cagtcttttt tattatttat ccgtttcttt
     1081 tacaaacaat tcttcgttag gactgttgta ataatttgaa ttcaatttaa gttctctgtt
     1141 ggaattgttt tttccaatta cggataatag catggggcta aaacctaata ttcctagaga
     1201 aatagaaccc agtaccaaca cagttttttt taacataggt tatacctcca ctttttttaa
     1261 cgctaccact tgtatacaca ttttaaatac tagccttcag tttatcaaat gtttaaattg
     1321 aacggatata aatcaaaatt aaattttcaa aagttgttct aaaaaatttt tcttttttgg
     1381 aagttactct aacttttttt gcttgtggtg cttggtatgt tttctccgcc tcccaggttc
     1441 ttgaccattt tctcttataa gtgggatttt taccacgttg agagaaaatg gtcaagaacc
     1501 tgggaggcgg agaatgccct taataccaag caccgcaagc ctttcccata ctttattcga
     1561 tgatcccacg atgatctcat aatgattcta caatgaccct acgatgatca aaattctcaa
     1621 aaaaaataaa cgaaatttca aacgattttt ttgaaatttt gttaaaatca catttgtttt
     1681 tttatgactt tttcaacaaa atcgattaga atttattttg tttttccacc aaaaattgag
     1741 tgagatttca acctatttta agcaataagt caccatatag tttgatagtt tacaactata
     1801 aacattgatt gtttacagtt gtaaactatt ttttctttct cataaaaatt tgagtgatct
     1861 ccccatcagg tagtacaact ttttcctcta ctatccgaac taacttctag aaaattttag
     1921 agattggttc tattagattt ccaaatttgc caccactttt tttcaataag tggttgtgta
     1981 ttgtctgctt ttttttcaga aaaggcagtt gattcggttg ttggagtttc ttctgtataa
     2041 gttaaatgga gctgttcttg taggttctca atttgttggt tcgcttgtag agtgagttgt
     2101 tgttgctggt cgagtaattt ttgcatttgt tttatttgat tatctttttc ttgtagctgt
     2161 atatcttttc gatttaactg tttttctagg aaagaaagga gttttaattc ggaaacttgg
     2221 ttggtgtttt tatggttttt ggtttttgat ttttcgccaa ctagttttcg atttaagttt
     2281 gcgttatcga ctaaaaacat tgattttata gctttttgac cggtttcgtt aatcacaaac
     2341 ttgtttccgt tttttgcaaa ccaaagtttt cgatttttgt cagaaacttt tttgttaatt
     2401 gctgttttac taactcctaa ttcttccgca agttctttga ttgttttatc tgccataaat
     2461 actccttaag cttaatcgaa taagttttgt aaatttccta gtaaatcttg tttgagtttg
     2521 tttgaaagtt ctcgtttttc tgcctgtttt tcttcagttt gttgttgttg tttaaaatca
     2581 gcttcatgag tgcctaaggg aagatttagt attcgatctt tagcgcgcca tttttcttct
     2641 cgtgttaatt ctccattgtg atcaatatta aataatttta tacgtaaatc ttcttgtttc
     2701 cctttggaaa aatcgtcagc atggttaatt tcagctttcc aagtaaaacg ataaccgata
     2761 acaggtttac ctcgaccttt accatatttt ttacgaatag ttaatccttt aaataaggga
     2821 gtgagttctt ctttaattgg ccttaagata aatttgtcaa tatttgacgg actattccaa
     2881 taacttttag gaatatctag taaatcaaaa aatttttctt tggaaaattc agtccagcct
     2941 tgagttcgat attgttttaa taatcgaaac atagtttttg aatatgtgct atccaaatta
     3001 gtaaattgct ctagattaaa tcttgtccag ttctttaatt gattaaaaag tcctttaaat
     3061 tctggattaa ctgaaattat aacaagttgt ttatctcgta taatgctata ttttgtgaaa
     3121 gcaacaaatt tagttaaagt atttccatca tcattccaag cattaatact taataatttg
     3181 tcatacgtgc tactaagatc ttttataaat tgttccccac gttctgtata accgcttagt
     3241 tttttcaaat cgtgaaatga aaaaataatc tcatctacac ctttttcacg tacacgtgaa
     3301 gcaattgaaa aaaataagtt catttcacga ctattaaatt ttctcagagg aatatgattt
     3361 aatctatttt catattttac aatttcattt gccataagct tctcctactt ttataaagta
     3421 tgaaataaaa gtaacatact ttataaaaat actcaataat gtcggaaaac ctacttgaaa
     3481 agcgtagaaa acctacttga aaagcgtaga aaacctactt gaaaagcgta gaaaacctac
     3541 ttgaaaaaac tctgaattgc ttgggagagt aaggacacag ccgacataaa agagatataa
     3601 aaagagatat aaaaagagat ataaaaagag gggaggctaa ttttcccgcc taaaaaatca
     3661 ctcctttttt ctctaaacag gttcgcgaat ttatcaaaac tgccaattcc aaaccttgct
     3721 ccctacggtt cgctaaaaag gtgtgatcgc tctgctctca cattatactg cgcagtcgcc
     3781 tccggctcct ttgctaaaag ccgttcataa ggaacccttt gagaagctct gagcgttcgt
     3841 ttaagacatt ttaagacagt cctagtataa atcccttatc ttgttcttaa aacgccttac
     3901 aggcggtcag ggcaagccct aacaacccta aaattgtcta aatgccctct aaaattcgat
     3961 ttaagcactc ttaaacctat tcttatatat ttatatactt aggcgtttca aacgtcttaa
     4021 aacgcaaatt agagcttgat aaagatattt gctcgtgtgc ctagactttt aaaaaagtcg
     4081 gcacacgcca tgacaaagct gaaataaact gcttcagctt tgtcatggcg gattcgctca
     4141 cttgaaaaag tgtttatttc gctcgattat tagcatatat tttttagctt ttttgctagc
     4201 caaattttgc attaatttgc tcataagtcc caccctttca gtacgtccta cggacgtatc
     4261 gttcgtaatg tgaaagtacc tttaaaggta ctttctacaa aactcactaa aagggtttgg
     4321 caagctctat atttgctagc caaataccta cgtatttggt gcaaataacc gcttgtcaaa
     4381 ggggcgcaag ctcccccttg aatcccccct caaaacccac gctccacaat acaaaaaagg
     4441 ctagcaaatg ctagccactt tttgtaaagt ttcgcttgtt tcaccttcgg tgaaattgtt
     4501 gctttgcaac gatcaggtag agctacattt tttttcgttg ttcaaatatt cttacgccat
     4561 ttactattaa aatcgtaggt attccaaagt aaagaaaaaa ttgattagca ggtaatacaa
     4621 aatataaaat aataccagca ataattacca aacttatagt aagttttgtt aatttcatga
     4681 agtatgccac cttatcttat atagatatca aatagattaa tatgccgatt gtattataaa
     4741 atccatgagc aactatggaa taccatatat tttttgtttg tatataaaca aaacttaaaa
     4801 taataccaat aaagaaaata cttaggcctc ccccatgaac aatagaaaac agaaaagaag
     4861 aaattagtat tgaaatatat tgttgttttt tgtttgcttg aggatcaatc atacaaaatc
     4921 gaaaaattcc ttcttctaaa aaagctccat aagtagcagc atttaaaaag aataaaagga
     4981 gagaaccatt tacttcatta ggatttatac cttctgaaaa agatgtattt agaatattga
     5041 ttaggaataa aacagagaat g
//
LOCUS       DAZULY010000187         4979 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000187 DAZULY010000000
VERSION     DAZULY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.189"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            60..2213
                     /locus_tag="ACG81A_002275"
     CDS             60..2213
                     /locus_tag="ACG81A_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFS5015601.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2182..3597
                     /locus_tag="ACG81A_002276"
     CDS             2182..3597
                     /locus_tag="ACG81A_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFS5015602.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            3630..4664
                     /gene="sppA"
                     /locus_tag="ACG81A_002277"
     CDS             3630..4664
                     /gene="sppA"
                     /locus_tag="ACG81A_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS5015603.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            4666..>4979
                     /locus_tag="ACG81A_002278"
     CDS             4666..>4979
                     /locus_tag="ACG81A_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFS5015604.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QT"
BASE COUNT         1808 a          692 c          949 g         1530 t
ORIGIN      
        1 ttaaagctat attgagaatt tttctataaa aattttttag aaggagttgt gttagataag
       61 tgataaaatc atttcctttt gtgcagcaac aagatgaaaa agattgtggt gttgcttgcc
      121 tttcgatgat tttaaaatat tttaaaactg atatcccgat tcataaatta cgggacctat
      181 ctgggactga tttagagggt acttcagcat ttggattgaa aaagacattt gaaaaattgt
      241 cttttgattg tatggctatt caagcagata gtgagatctg gaaagaaaga gatcttatct
      301 taccattaat tgcacatata ttggttgata acagctatat gcactacgtt gtaatttata
      361 aaacgaaggg aaacgtacta tggattgcag atcccgctaa tggaaaaaat agaaaaacga
      421 ttgaagcgtt tcaaaaagaa tggacaggta ttcttttatt acctatgcct agagaaactt
      481 atattcctat taaagaaaaa gtagctggat taagttcttt tttgcctata atttggaaac
      541 aaaaaggatt agtattccac atcgttttag catcgatttt cattacgttt tttggcatag
      601 gaagctcata ttattttcaa ggtattcttg attttttcat ccctaatcaa gcacgttcaa
      661 ctttaaatat aatttccatt ggcttgatcg ctgtctattt atttcgggta ttgtttgaat
      721 atagtcgtag ttatcttctt attattttag gacagcgaat gagtatggca attatgcttc
      781 agtatttcaa acatgtgtta actttaccaa tgaatttttt tgctacgaga aaatcagggg
      841 aaattatttc tcgttttctt gatgccagta agattattga tgctttagca agtgctacgt
      901 tatcaatatt tttagatatt ggtatggtgt tgcttgttgg ggttacgtta gctattcaga
      961 atagtacgtt gtttttaatc actctatgct ctttgccttt ttatgtagtt gcaatattcg
     1021 ctttcgtgaa aagctatgaa aaatcgaaca gggaagaaat ggcagccggt gcagctgtaa
     1081 attctagtat catagaaagt cttaaaggaa tagaaacaat taaagcatat agcggtgaag
     1141 agaaagtata taaccgtata gaccatgaat ttgttaaatt aatgaaaaaa tcatttcgta
     1201 cggttacgct tgataatatt caacaaggaa ttaaacattt gattcaatta attagcagtg
     1261 cattaatttt atggctaggt tcttattttg ttatggataa tagaattagt ctaggtcaac
     1321 ttatcacata caatgcttta ctagtatttt tgacagatcc tttacaaaac attattaatt
     1381 tacaagtgaa aatgcaaacg gctcaagttg caaataatcg tttaaatgaa attttcgcga
     1441 ttgattcaga aaaatcgaac cagtcttttg aatatatgct ttctgaacaa ttatttcaac
     1501 aagatatcac tgtaaaaaac gtttcattct cgtatagcat gaaggcccca acgttgaacg
     1561 aaatttcatg tacaattcct tcgcacagta aaacggctat agttggagtt agtggttctg
     1621 gaaaatcaac tcttgcaaag ttatttgttc gtttttatga agcatcagaa ggatcgatcc
     1681 attatggaaa cattaatcac ttagacattc cttttcagat attacgcgat cgagtgacat
     1741 atttgcctca agagtcattt ttctttagtg gaacaatcat tgagaatctt atattcggtt
     1801 tagatagccc accaagtttc gaacaaataa tagaagtttg tgagatagtc caactaaatg
     1861 aatttattaa tcaacagcca ttacgttacg agacattgtt ggaagaaggt ggaaataatc
     1921 tttcaggagg gcaacgacaa cgattagcct tagcacgggc tttattaaaa aatccggaca
     1981 tattaatttt agatgaagca actagtggat tagatacctt gttagaacat gcaattatga
     2041 attatctcct caatttaaaa gaaaaaacga ttatttttat tgcgcatcat ttaccaatag
     2101 caaaagcatg tgatcaaatt ttagttctcc atgaagggca tttggtagaa aaaggaacgc
     2161 acgatgaact tcgttttgcc gatggtatct atcaaaaatt atgggaaatc taggagaggg
     2221 aaacaattta tgcagaataa taattggttc gaacattcta gtatctatac taaacaacat
     2281 catagcttct accgttgggt gttataccct gtaattcttt ttttatgtat tattggattg
     2341 tttttaacct ttgctaaaaa agaggttgtt attcggacaa cggctcaatt gaaccccgaa
     2401 aaaatagaga agttacaagt gccattagaa gctaaaataa tggaaaactt cctaaaagaa
     2461 aatcaatttg ttcataagga cgatgtgttg gttagattgg attgttcttt aatagagaat
     2521 gaaaaggctc agatagaaca agaaaaccaa cgtattactc aacaaataaa gatggctcag
     2581 ctatttattg aaagtataag taaaggaaaa aatttgtttt caacggatga cagttttggc
     2641 tacagtaatc aattaaagag catgttgtca gaaaaagaat cactccgcta cgctttgaag
     2701 caaagtgaat taaatgatca aaagcaatta gaagtatacg aaaagacaaa aagacaacta
     2761 gaaaaacaaa ttgagagttc agatagtaaa ttacaagaat ggcaacaagt acaggtagct
     2821 tggagtaata atcaatcatt aaaagatttt tcaaaagaaa tgatggcaaa ctatgagaat
     2881 tggcaagaac aactaaataa tgtttctgat gatcaaaaaa atcaagtgaa actgacaatt
     2941 tcagcaagca taaatgaaca aattgagcaa ctaaaaaaag aagtagaaca gtatcagtca
     3001 gaaaaagcta aattagttaa accaactact tctgagaatg acagaattag tcaaacggaa
     3061 aaaggaaagc aagagctaga acaagcaata acaactgtaa aacaaacagt tacagagcta
     3121 aatgaaaaac aagagaagaa ccagtcagtt atcaaaagtt tggacgatca actttcaaaa
     3181 ggaatactaa aagctcctgt atctggaacg gttcatttaa atgaagaaac aaagggacaa
     3241 gcagaactag ccaaaggaac agtgcttgca gaaatttatc cgatccacga aaaatcaaaa
     3301 atgaaattta cagcactttt acctgcgaat gaaagtacct atattaaaga agggatgaag
     3361 gttcatttta aattggatca aaaaggtggg gctccgatta cgatcgacgg aactttagat
     3421 gaaatttcag aaaattctac tgccactgag aaaggtgttt tttatgcagt gaaaggaacg
     3481 ttaaaaacaa ctcaatcgtt tccgtttcgt tatggattaa ctggagagtt gtctttaatt
     3541 gtaggagaaa aaacatattg gcaacaaatt gtcgatgtgt ttctaaataa aaaataatta
     3601 tttttactaa gaatatagga ggagtttata tgaataaaag aagatggatt gctgtgggag
     3661 tagcgtgcgg attattgtta ctatccatta tcgtcgcgct aattccagga aaagataaag
     3721 aggaagcatc aaatacaacc ctaacaggaa taaataagat tttttacgga tctaacgaaa
     3781 tcacagaaga aacgttagaa gaaggcgcat caaataaaaa gattgtcaaa ctatccgtca
     3841 atggagttat tgcagataca ggtgaaagca atctttttag tagggaacaa tataaccatc
     3901 agaatttttt aacacagcta aagaaaattc aagaagacaa agcggttaag ggagtgttat
     3961 tagaagtaaa ttcacctggg ggaggaatat atgaaagtgc tgagattgct aaagaaatgg
     4021 caaaaattaa aaagctagat attcctatct atactgcatt taaaaataca gcggcaagtg
     4081 gagggtacta catttctgct ggttcagata aaatatttgc tacggaagaa acaacaactg
     4141 gatcaattgg tgtcattatt tctggtttaa attattctgg cttattagaa aaacttggcg
     4201 taactgatgc gacgtataaa agtggtgcat taaaagatat gatgtcacct caacacaaac
     4261 cttcagagga agagaataaa gttatacagg aatttgtgat gagtgcttat gatcgttttg
     4321 tcaatgttgt agcaaaagga agaaatatgg atactaatgc agtgaaagaa cttgcggatg
     4381 gaagaattta tgatggtaac caagcagtag aaaatgggtt ggtggatcaa attggttact
     4441 ctgaagatgc attagattcc ctaaaaaaag agaagaaatt aactgatgcg acggttatag
     4501 aatataaaaa tgacacaact ggctttgctt cttcttggtt aggtaataag attgctgaat
     4561 ggcaagggct aaaggcaact acatcagatc gtgtaatttc agtttttgaa aaacttggat
     4621 tttctgatac tccaaaacca atgtattact atggagggct ataaaatgaa taagaagagt
     4681 ggcattgaaa ctttgcctaa ttatgtattt gcgggatttt ggttacgatt ttttgctttt
     4741 cttattgatt taatttgtat tagcatcatc acccatgcta ctataggtct cttttacaaa
     4801 atcatgggac tggaatctga caaatccatt attacttttt atggtatagc gtcattagct
     4861 atttatttaa cctattttgt acttttgact aagctgaatt caggacaaac aattgggaaa
     4921 atgatttttg ggataagagt agtgagcttt gaggaaaaag agctaagttg gcaaactat
//
LOCUS       DAZULY010000188         4946 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000188 DAZULY010000000
VERSION     DAZULY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.190"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            27..845
                     /gene="purR"
                     /locus_tag="ACG81A_002279"
     CDS             27..845
                     /gene="purR"
                     /locus_tag="ACG81A_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFS5015605.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1348..2721
                     /gene="glmU"
                     /locus_tag="ACG81A_002280"
     CDS             1348..2721
                     /gene="glmU"
                     /locus_tag="ACG81A_002280"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFS5015606.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2815..3786
                     /locus_tag="ACG81A_002281"
     CDS             2815..3786
                     /locus_tag="ACG81A_002281"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS5015607.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3792..3894
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3960..4529
                     /gene="thiT"
                     /locus_tag="ACG81A_002282"
     CDS             3960..4529
                     /gene="thiT"
                     /locus_tag="ACG81A_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFS5015608.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4610..>4946)
                     /locus_tag="ACG81A_002283"
     CDS             complement(4610..>4946)
                     /locus_tag="ACG81A_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015609.1"
                     /translation="IFASKLSSILSDKINVKKSFFKKETPVEKISVELLKEMRNLMQE
                     GIIFSFDQVGLIDGKYRIPIYVIEDYIWKLKGTRYREEEIIATLIYSVTDRHWQLVED
                     ADLSYLPLK"
BASE COUNT         1610 a          875 c         1078 g         1383 t
ORIGIN      
        1 aaatttctta aaaggagttg gaataggtga aagtacgtcg cagtgaacgt ctagttgaca
       61 tgacacagta tttattgaat cacccgcatg aattgatttc cttaacaagc tttgccgaac
      121 gttatgaatc tgctaaatct tctatcagtg aagatttggg aatcatcaag aaaacattta
      181 aagaacgagg gtatggaacc ttggaaacaa tcccgggtgc tgcaggtggt gtattgttca
      241 tccctgaaat ttcatatgaa gaggcgaaaa aatatattga atctctagca gaacgtctat
      301 cagaacaaga ccgtctgctt ccaggaggat atgtttatct ctctgatttg ttaggagaac
      361 ctgaactgct gcgtcaagtt ggaagaatca ttgcttctaa ataccttggt gaaaaaatcg
      421 atgctgtaat gactgtagcc acaaaaggcg tacctattgc ccaagctgtg tcctattatc
      481 tgaatgcgcc attcgttatt gtacgccggg actcaaaaat aacagaagga tcaacagtaa
      541 gtgtcaacta tgtgtctgga tcttctgaaa gaattgaaaa gatggaactg tctaagagaa
      601 gtttgaaacg tggttcacgt gtccttgtag tggatgattt catgaaaggc ggcggaacag
      661 tcaacggaat gaagagcttg attgaagaat tcgaatcaga acttgttggc atcactgtat
      721 ttgccgaatc aaaattcaac ggtcatcgcg ccatcgatga ttatacgtca ctgctttatg
      781 tcaaagatgt agatactcaa acgaaaaata ttacggttgt gccaggaaat tatttttcag
      841 aataagaggt tgtgaaaaag ccgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
      901 gctttccaca ttttttcata aatttggctt aataccgaga aactggcttt tgaacaccgt
      961 ttatctagag gttgtgaaat cagcgtcctg tcctgagtat tagctcaata aacggaaaaa
     1021 ttgctcctca tattttgccg tttattgagc taataccgaa ggtcagctga ttgaaaaacg
     1081 tttaatgaag aggttgtaag cctaccgttt aactctaagt cacaagaaac gatttcaaaa
     1141 aacagctttt catgttttat gagtagtgtg acttgtgacc gaggagttgg caggagagca
     1201 ccggttacca aatagatagg cccgttatgg tctatctatt tttttatcca gaaaacgctg
     1261 ggataaaaga aaatcacttg ttttgttgaa aaaatgggta tactgggata aactgaaatc
     1321 agcaaactat atttaggagt gacgcagttg gacgcacgtt atgcaattat tttggcggca
     1381 gggaaaggta cccgcatgaa gtcaaaatta tataaagttt tacatcccgt ttctggtaaa
     1441 cctatggtag aacatatcat taatcgggtc agtgaaacga aaccagatga agtgattacg
     1501 atcgttggac acggagcaga acaagttaaa gcacaactag gagaacgcag taaatatgcg
     1561 ttacaagcag aacaattggg aacaggacat gctgttttgc aagctgcttc tttcttggaa
     1621 ggaaaaaaag gaacaacgtt agttatcagt ggcgatacac cattattgac tactgaaacg
     1681 ctgaacaatc tttttgaata tcaccaagga aaaaatgcaa gtgcaacgat cttgacggca
     1741 caagcagaaa acccaacagg atatggacga atcatccgtg accatattgg gatcgtagaa
     1801 aaaatcgtgg aacaaaaaga tgcaacacca gaagaagctc ttgtacaaga aatcaataca
     1861 ggaacttact gttttgacaa cgaagctttg ttcgatgcgt taagcaaagt tggaacaaac
     1921 aatgctcaag gtgaatacta tctaacagat atcatcgaaa tcttaaaaga agaaggccat
     1981 acagttgctg cttatcaaac ggatgatttt gaagaatcaa tgggagtcaa tgatcggatc
     2041 gcgttagcta aagcaaatga gatcatgaga aagcgaatta accagatgca tatggtcaat
     2101 ggcgtaagct ttgtcgattc agcaacgaca tacatcgatg caggcgtaga aatcggtcca
     2161 gacacattga tcgaagcagg tgtacaaatc caaggaaata cagtcattgg ttctgattgt
     2221 gtcatcgggt cacattcaaa aatcgtagac agtaggattg aggatcacgt cgtcattgaa
     2281 aactcagtca ttgaaagcag ccatgtcaag aaacatgcag atgtgggacc ttatgcacat
     2341 ttacgaccaa aagctgaaat tggcgaaaac gtccatatcg gaaactttgt ggaagtgaaa
     2401 aatgctcaaa tcggcaaagg aactaaagtc ggtcacttga cttatgttgg tgatgctacg
     2461 ctgggagaag aaatcaatgt cggatgtggt gttgtcttcg tcaactatga tggaaagaac
     2521 aaacatcata cgactgtcgg cgaccacagc tttattggtt ctagcacgaa tatcatcgga
     2581 cctgtcgagg tagctaaaaa cagttcgatt gctgccgggt caacaattac agacaatatt
     2641 ccagaatatg cgctggcaat tgcgagagca agacaagtca ataaagaagg atatgcaaaa
     2701 aaactccctt atctaaacta agacttttgc aaaatcactt gatttattta aaaaatatga
     2761 ctactattta ttaggtagaa ttctagagtt gtgaaaaaga aagtggaggt ccccatgtca
     2821 aaacattact ttgacccaag attaaaaatt tttgcgttaa attccaatcg cccattagca
     2881 gagaaaattg cagcagcagt cggcgtcgag ttaggaaaat catctgttac tcaatttagt
     2941 gatggagaaa ttcaagtaaa cattgaagaa agtattcgtg gatcgcatgt ctatgttatt
     3001 caatcaacga gcagcccagt caatgataac ttgatggagt tgctgatcat gattgacgca
     3061 ctgaaacgag caagtgcaaa aacaatcaac gtagtcatgc cttattatgg ctatgcgcgt
     3121 caagaccgta aagcacgtgc acgggaacca atcacagcga aattagtagc aaacatgatt
     3181 caaaaagctg gtgcaacaag aatgctgact ttggaccttc atgcagtcca aatccaaggt
     3241 ttctttgata ttccagtcga tcatttgatg ggtgcgccac ttattgccaa ttatttctta
     3301 gaaaaaggaa tcaaaggcga tgatgttgtc gttgtttcac cagaccatgg tggtgtcaca
     3361 cgggcacgta aattagcgga atttttgaaa tctccgattg cgatcataga caaacgccgt
     3421 ccaaaagcaa atgttgcaga agtaatgaat atcattggac aagtcaaagg caaaacttgt
     3481 gtactgatcg atgatatgat cgatacagca ggtacgatca cattggctgc caatgctttg
     3541 aaagaagctg gtgcaactag tgtgtatgct tcatgtacac atccagtatt atccggccct
     3601 gctttacaaa gaatagaaga ttcagctatc gagcgtctag ttgttacaga ctcgatctat
     3661 cttccagaag atcgtaaaat cgacaaaatc gatgaagtaa gtgttggcgg actaatggga
     3721 gatgcaatca aacgtatcca tgaaaacaaa ccagttagtc cattgtttga aacaaaaaat
     3781 aaataataaa taaactcaca aaggggagtc caattgggct gagattgaat ctatttctaa
     3841 acccttcgta cctgtatcgg ttatgcgagc gtaggaattg tgaataaaca gctgtttgac
     3901 tattttcgaa agctgcttcc tcctttgtga cttttttatt caaaggagga agttttttta
     3961 tgggaaaaaa tagagtttgg gtagaaggaa cgattgttgc agcgttagcg atggtgttgt
     4021 cgttgattcc gatccagatc ggcagcagtt tttctatttc attagggcag attccattga
     4081 ctctttttgc gttaagaaga gggacaaagg cagggatgct ggcaggtttc atttggggca
     4141 tcctacattt tccgttggga caagtttatt atttaagtgt cgttcaagtg ttgattgagt
     4201 acccgattgc ctatactttt gcaggcgcag cagggctcat ggcttcaaat gtccaaaaag
     4261 gctatgtaga cgataatacg aaacaaatta gaaaaagcat cgtgtttggt gcattgattg
     4321 gcgcactttc cagatatttt tggcatttta ttgcaggagt ggttttctgg ggagcgtatg
     4381 cactatgggg gatgaatcct tggctctttt ctttcgtaat gaatggtgcc agcgggttga
     4441 gtacagctat tgtaacgatc atcgttttat tagggatcca acaaacgact ccttcgattt
     4501 ttgtgccaga taaacgacag gagctgtaaa taaaaaagaa gcaagaaact tgagacagaa
     4561 tcatgtccta gtttcttgtt tcctttttat tttttgataa gaaatagatc tacttcaatg
     4621 gcagataaga aagatcggca tcttctacca attgccagtg tcgatcggtg acagagtaga
     4681 ttaaagtagc gatgatttcc tcttctcgat accgggtgcc ttttaatttc caaatataat
     4741 cttcaatcac atagattggg atacgatatt taccatcgat caaaccaact tgatcaaaag
     4801 aaaaaatgat cccttcctgc atcaaattac gcatttcttt caataattcc acactgattt
     4861 tttcaacagg tgtttctttt ttgaaaaaag atttttttac attgatctta tctgacaaga
     4921 tcgaacttaa tttactagca aaaatc
//
LOCUS       DAZULY010000189         4946 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000189 DAZULY010000000
VERSION     DAZULY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.191"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            42..464
                     /gene="mgsA"
                     /locus_tag="ACG81A_002284"
     CDS             42..464
                     /gene="mgsA"
                     /locus_tag="ACG81A_002284"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFS5015610.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      651..749
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            787..1323
                     /locus_tag="ACG81A_002285"
     CDS             787..1323
                     /locus_tag="ACG81A_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFS5015611.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(1363..3126)
                     /locus_tag="ACG81A_002286"
     CDS             complement(1363..3126)
                     /locus_tag="ACG81A_002286"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5015612.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(3129..4844)
                     /locus_tag="ACG81A_002287"
     CDS             complement(3129..4844)
                     /locus_tag="ACG81A_002287"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5015613.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         1503 a          968 c         1002 g         1473 t
ORIGIN      
        1 agtaaataag tatgttaaca atatcaaaag gagtgtctct tatgaaaatc gctttgatcg
       61 ctcatgaccg aaaaaaacct atgatggtga aattagcaac agcgtataaa acaatattgg
      121 aaaaacatga actttttgct accgggacga ctgggcagag gatcacggaa gcaaccggct
      181 tgccgatcca tcggtttaaa tccggaccgt taggcgggga tcagcaaatt ggagccatga
      241 tttcggagga tcagttagat ttggtgattt ttcttcgaga cccgctggct gcacagcccc
      301 atgaaccaga tgtaacagca cttatccgtt tatctgatgt ctatgagatt ccgttagcta
      361 cgaatatcgg aacagctgag gtgctactaa gaggactgga agccggcttc ctagattttc
      421 gaagaatcgt ccatgaaata gacaagcatc cactttcatt ttgatggata agcgtatgga
      481 aaaataacgt ttaatgttcg cctatatgta gctataaaag ctaaacaaac tgataatatg
      541 cgatataata cgaacgtatt ggataatata aacaataata ttagtttttc gtatttgcag
      601 taaatgtgaa aatatgctat agtttctgtg ggaaaagatc ccaaaaataa tacagagtag
      661 aaaataaagc gttaagtgct aagaggatgg gatgttgcct cttggacgaa ggacgtaatg
      721 ttcgcggttt tattttcgca ttcgctgcat gatacgatgt agcagctctt aaaggagatg
      781 ctaaaaatga agaaacaccg gttagacgca cgtatgattg ctattatggg cttattgatt
      841 gcattgatgg tcactttgtc ccgactggta gcaatcgaga ccccatttat taaaatcagt
      901 gtcacattta ttccacaagt gatcatgggc attctttttg gtcctttttg gtcagggata
      961 ggcgcagttt tagctgattt agtaggaatg gccctcttct cgaaatctgc attctttatt
     1021 ggatttacat tgaatgcatt tattgaagga gcaatctacg gattcttttt ctatcgaaaa
     1081 gagatcactt ggaaaaatgc cattctggct acgcttagtg tcacgctgat catcaactta
     1141 tttttgacac cattatggct tgcgttgatg taccatgtgc cattgttcag ctgggtcgtt
     1201 tgggcgcccc gcctgttaaa aacagtaata tggttaccga ttcaatcaat tgccatttat
     1261 tacgtaggac gtagtattcc ttacaaaaaa attttaagaa gtttagcgat ccacgctaaa
     1321 taaaaaacaa gcagtcttcc catcccagaa gactgcttgt ttttattgtg ctgtgaattg
     1381 tgcttgatag agccgtcggt aataaccatc tttttgaagc agctcatcat gggtgcccat
     1441 ttcgacaata gctccctgat ccatcactaa tatctggtcc gcatttttga ttgtggacag
     1501 gcgatgggca atcacaaaac tggtttttcc ttccatcatt gctaagaagg cttgttggat
     1561 ttttttctct gtcaatgtat cgactgagct ggtagcttca tctaagatca acataggggg
     1621 attactgatc atcgttcgag caatcgtcaa cagttggcgt tgtccatctg atatctttaa
     1681 cccttgaccg ccaatcttag tatctaattt ttccggcaaa cgttctacaa attcatacat
     1741 ataagaggct tttagggcat cattgatttc tgcgtcactt gcttccggat tcccaaaacg
     1801 aagattttct cgcaatgtac tgtcaaatag ccaagtatct tgtaacacca ttccaaaact
     1861 tttacgtaat gtatcacgtt ggatagagcg gatgtcatgg tcatcgactg taattgttcc
     1921 ttttgttgtg tcataaaaac gcatcagcaa attaacaagt gtggattttc cggcccctgt
     1981 ttttcctact atagcaatgg tttgacctgg ataagcagta aaattgaaat cttcgatcaa
     2041 acgctgcttg gggtcataag aaaaagaaac atgatcaaaa gagacttttc ctttgatatt
     2101 tttgggttcc aaggcatagg catctggcaa atcaggcttt tcttctggct gatcgatcaa
     2161 atcaaatgct cgttctagac cagaaaaagc ggtttgcagt tgtgtcgtga ttccagaaag
     2221 ttcgataaat ggtttagaaa actgacttga gtagatcgta aagctggaaa tcacaccaac
     2281 agtgattgac gaaccactct tcaatgccaa taggccgcca actaaaccaa tcgataaata
     2341 agcaaggtga tctacaaaac gagaagacgg gtttgtcaag gaagaagaaa attgtgcccg
     2401 ttgtcctttt tcatatagat cagcatttag cagttcaaat tgctgttgac tagcttcttc
     2461 ttgctgaaaa gctttcacga ttttctggtt accgattcgt tcggtcacaa agcccgagat
     2521 atccccaata atcctttgct gtctatcgaa atttgtttgt gaagcacgag caacgatcca
     2581 actcacaagg aaaataattg gtgtagaaca aagaacgacc aatgtcagaa gtggacttag
     2641 acgcatcata aaaataaacg aaagaatgat gattgccact ccagaaaata attgattgaa
     2701 ggccgcagaa attgcaatgg aaatattgtc catatcattt gtaaatcgac tgacgatatt
     2761 tccgtgggag ttttgatcat aataacgaag tggcaatcga ttcagatgag caaatgcatc
     2821 ttttcttaat tttgcaacgg ataagtaagc taaccgattg cttaacactt ggatgagcca
     2881 ttgactgact acgttgatta aaacgatacc accgaataga ataagtaaat ggaacagtcg
     2941 attgaagcgg acctgtcctt ctccaatcat ttgatcgata ctttgaccaa tttgtaacgt
     3001 catcacaact gttgcaatcc cattgatgat tcctagtatc acagctagcc aaatgtcttt
     3061 tgcataggca gtaaggtaag gaatgaatcg gcggagcgtc tgcaaggata atttttttac
     3121 tttcataact attcttcctc ctgtgatgcg acgatttcct gatattctgc tgattgagca
     3181 agtaattctt catgggtacc ctgagcagca atcttacctt gatccatcac taagatatga
     3241 tgtgcttctt gaatagagcg gacacgttgg gagataataa tcactgtact gccaagataa
     3301 gtctgtagcg ccttacgcaa atccaagtct gtttgataat ccaacgcact taaagagtca
     3361 tctaaaatca gtaatgctgg cttggcaatc aaagctcttg caatcgtcag tcgctgtttt
     3421 tgtccgccag agaaattttt cccattttcc atgacttttg tatctaaacc atttggaagc
     3481 tgacggacaa attcttctgc ctgagcgatt ttcagagctt cccaacattc tttttctgtg
     3541 gcgtctttct taccccattg aaggttttca cgaatagtcc cggtaaatag tacagaagtt
     3601 tggggaacaa gagcaatttg ctgacggaga tgatccagct gccaagaacg aacatccatt
     3661 ccgtccatta taactgtacc ggaactgaca tcatagaaac gcggaatcaa ttgtgtcaaa
     3721 gtcgttttcc cgcttccggt tggaccaatg attccaagga ctgtatttcg cttcagctga
     3781 aatgtaatat tttggagaga aagaccagaa tcaggagtat aacggaagtc aacatggtca
     3841 aaagcgataa ttgcttcaga agaaaaatcg ggagtagtac tgacctcagc ttcaagaatc
     3901 gaattttctg tgtctagcac ctctttcacc cgatttcctg aagcttcggc acgtgtgaag
     3961 atcacgacga ggtttgaaac aacgatcagt gccaaaagca tttgattcat ataattgatc
     4021 aaagcaagaa cttgtccttg ttgcaagcta ccaatattta ctttgatccc gccaactgtc
     4081 agcaaacaga tgatcccgac attcatgatc aaggttgtag caggtgataa tagggcagag
     4141 atattggaaa cacgtaaata attgttcgct agatcatttg tgcttttatc gaacttctct
     4201 gtttcttttt ttgtctgcgc aaatgcgcgg atgacacgaa caccgctaag attctgacta
     4261 acgtgttcat tgagctgatc tagtttttct tgcacttttt ggtagagagg aacggttttt
     4321 ttgatgataa aaaataaaat gattgagaaa ataggcaata atgctaagaa aaacagacct
     4381 acttcccagt cgatcacaaa agccatgacc actgacccga tacttaaaaa cggtgctctg
     4441 atcaataaac gaatgaccat tgctaaagct agctgaagct gattgatgtc gtttgtcatt
     4501 cgggtgatca aggtatctgt tccaaaatga ttcaactcgt tgtgagagaa agtattgatt
     4561 tttttgatta gctgattacg taactctgta ccaaatcctt gtgaagctac tgaagcatag
     4621 tattggcaaa tgatagcaca aatcagacca atcacagaca tagcgaccat ccataagccc
     4681 atttcaatga tttttcctcg gtcattcatt tttagtccat tatcgatcaa ggaagccatc
     4741 attaaaggca ggacaagttc gaagacagct tcaagaaatt tgaagaacgg gcccaaaatg
     4801 atttgcttac gatagtcttt tgcataacgt aataactgaa tcatatgatt cctcctatta
     4861 tttatttgtc cacaattgaa aaaacaagta ctaccacatt gtagcttagg aaatttagaa
     4921 tgaaaagaga acaagccttt tcataa
//
LOCUS       DAZULY010000190         4945 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000190 DAZULY010000000
VERSION     DAZULY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.192"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..722
                     /locus_tag="ACG81A_002288"
     CDS             <1..722
                     /locus_tag="ACG81A_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015614.1"
                     /translation="VFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYTMSI
                     VIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAENYNH
                     TDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTPIYR
                     YKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTLILF
                     LLYRFTFSKQKQPRREVQHSH"
     gene            complement(867..1607)
                     /gene="truA"
                     /locus_tag="ACG81A_002289"
     CDS             complement(867..1607)
                     /gene="truA"
                     /locus_tag="ACG81A_002289"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS5015615.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1798..2433)
                     /locus_tag="ACG81A_002290"
     CDS             complement(1798..2433)
                     /locus_tag="ACG81A_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFS5015616.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(2580..3800)
                     /gene="sstT"
                     /locus_tag="ACG81A_002291"
     CDS             complement(2580..3800)
                     /gene="sstT"
                     /locus_tag="ACG81A_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFS5015617.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            4019..>4945
                     /locus_tag="ACG81A_002292"
     CDS             4019..>4945
                     /locus_tag="ACG81A_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015618.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVS"
BASE COUNT         1628 a          963 c          745 g         1609 t
ORIGIN      
        1 gtgtatttgt tcttttttgt tcaaccaatt tatttccttg gcaacttttt aaccatacac
       61 cagttaagag tctacaattt gtctggcggt taaatggttt ttcttcttta tttatagcct
      121 atacgatgag tatagtgatc tattatattt tttctactaa aaataattca tggaaaataa
      181 tggtctttac gtttcttgca ctaatattgc atctctcagg agtaagcaat tctatccatg
      241 ctaacaaaga ttcattaaca cttatagcac ctgaagacgc tgttgctata gctgaaaatt
      301 acaatcatac tgattatgca aacaaagagt caatctatca tcctgatatg attaataatg
      361 atcaatattt actaggtaac caggaaataa atccaacaac tcattttatg agtaacaaac
      421 ttacaattga attagataat tcgaataata aaaatactgt cttgacgaca cctatttatc
      481 gttataaagg gcaagtggct tctattaatg gcaaactagt acaaacaaaa ttatctaagt
      541 ttggcacaac tgaattaact attcctccag gtataaataa agtcgttatt acttatcagt
      601 acacaaaact agcaattgct tcaagatatt tatctatcgt tactttgatt ttatttttac
      661 tttatcgatt tactttttct aaacaaaaac aaccgagaag agaagtacaa cattctcact
      721 agatttttat ttaagaaaga ctttttagct gtacacataa acaggctaaa aagtcttttt
      781 ttatatttct ttaatttgct tcatacatta tttaacatcg atatttatac tgtgaaacat
      841 gcgtttcatc cattatcaaa gtatttttat cgataataaa cttcatccaa aaacagacct
      901 tgtgcaggga cggtttctcc tgcatgttgc cgcactttgc tttcaaatat ttcatcgatt
      961 gtttctatcg gcatggcacc tgtgccgatt tctagtaatg tccccatgat aatccgcacc
     1021 attttatata agaatccgtc tccgacaaaa gtaaaatgaa gcatttctcc ctttcgttcg
     1081 atagataatt catcaatcgt acgcactgtg gatttttttg attttttcaa tgcagaaaaa
     1141 ccaatgaaat catgtgtacc cactaatttt tcgcaagctg cattcagctt gtccatatcc
     1201 aactcttgag ggaaataaaa gctgtggttt cgctcaaatg ctgaaggaat cacgttgttc
     1261 caaacataat agctgtattt tttgcccacc gcattatatc ttgaatggaa gcgctcaggc
     1321 atttcttcga ttcttttgat gacaatatcc cgcggaaggt aacgattcat aaagtcgatc
     1381 atttctgcca gtccaatttc tgtgtttgtt ttaaaatttg ctacttgtcc tcgcgcatgg
     1441 gtgccagcat ctgttcttcc tgatccaata atttctatct cttcatttgt catctgatgc
     1501 aatacttttt cgatttttcc ttgaatggtt ttatcgtcat ctccaagtct ttgccatcct
     1561 gaatatcttt ttccgtcata ttcgattgtc aacttgatgt ttctcatatt ttagtggctc
     1621 ctctgtcttc catttggtct tctctctacc tttttaagat tgtagcacaa aatatagctc
     1681 cttgcttctc taagacagaa gctatacaag aaaactgtgg aaatatatat ttcagaaaaa
     1741 caaaaaacag ccaccggatg aagtatccgc tgactgttca atttgacggc tcaagtttta
     1801 ctgtttgaga tgcaagatac tcaacctggc aatgggcaca taattaaaga aaaaactgat
     1861 tctttggcaa aaacctattt tattcagtaa tggccaagaa tttatagaag gaattcttgc
     1921 cagtccataa atcgccgcaa aaagcaaact gatcacagac agtccaaggt ataagtggct
     1981 tacgtccatt tgcccatttt ttcgatagat caagaaaaga agaactggaa acaataaaaa
     2041 accactgtaa cctaaacctg atccgatatt atggacccat gtcgagacat tccacgaaga
     2101 ttcatgggta ttgatactga acaaacctgt aaagatacaa tcacctatac catagaacgc
     2161 gatggctgct gtaactaggg tagctaacac acgagatgta ctaacaaatg cagcatacaa
     2221 tgcaggcaat gacaggatga aaaagatccc tgaaaccaca gaccaaataa gaaacgcttt
     2281 tcttacagga ctggaaacct ctccaaatac actgatcacg ctcttcattt gatctaattc
     2341 aggataatac aatcctaata cgtacggagt caaaaaatca ctcagcacgc ctaataataa
     2401 aaagaaaaaa ccaaatttac gtaaaaaatc catgatacgc ctccaataac aattacctat
     2461 gcttttaatt gttattataa acgactttat cttgatttaa cagaaacctc tatttacacg
     2521 aacatctata taactcatta aaaactcgaa tcattttaaa aatactaaat atctaacgtt
     2581 caacgttcac ttaatccatc taaacaatct ttcaatttga ttttttcccc acttctttta
     2641 cgcgcgccta gctctcctat tgcagtaaac aacagatcac ttgacgaatt taaggcagtt
     2701 tccattgaat cctggataac accgataata aatccaatac cgacgatttg catcgcgaca
     2761 tcattagaga taccaaacaa actacatgct aatggaatca ataacagtga accgcccgca
     2821 acacctgaag ctccacaagc agctaaagca gacaaaatac ataaaaggac tttcaaaaca
     2881 aaaggtgctt caatccctaa agaatgtaca gcagtcaacg tcatcaagga aatggtaatc
     2941 gcggcaccgc ccatattgat cgtagcacct aacgggatcg atatggaata agactctttt
     3001 gtcaatttca atctttcagc caacatcatg tttatcggga tatttgcggc agaacttcgg
     3061 gtaaagaaag cgggaagtgc gctttctttc aaacaaaaga aaatcaatgg atagggattt
     3121 tctttaataa acaaaaacgt gaggaatggg tacacaacta atgcgacaaa cagcatcgta
     3181 ccgatcaaaa caagtaaaag ctgcagatac tctgccaatc cagcgattcc agtcgtagca
     3241 attgattgat aaaccaatcc taaaatccct atcggagcaa atgcaataat cacttgtacg
     3301 acttgtgaca acgctataga tatctgatcc accaactctt ttgtcgattc tgaacgtgct
     3361 cgaaaagcta gtcccaacaa tacagcccag aataagacgc caaggtaatt cgcatcaata
     3421 agagattgaa caggattttg tgtgacattc gtcaacatag tagataaaac actttccaaa
     3481 ctttgcggtg ctttttcaga tactgtttct tgtaaagtaa taggaacagt aaaaattgaa
     3541 cttacaataa ctgctacaag tgcagaaagc aatgttccca ctaaatataa gatcaaaata
     3601 ggcttcacga atgtcttatt cccaatttta tgcttggcaa ttgacgccat cgtcaagaaa
     3661 aaaaccagaa taggtgcgat tgccttcaag caactgacaa acaacgttcc caatattgaa
     3721 ataaatgtcc atgtggggaa aaagatacca aacaatattc cgactaaaat accaacaaca
     3781 atacgttgaa ctaacgacat atccaaaaac ttctctaaca aatgaccacc ctttcacaat
     3841 tttaacaatc attgtatcat tttatgcaaa gaaactaaag gtataatttt ctaaaaattc
     3901 taacaataca aacaaccatt tcttgctaat tttgtacttt ttctgtctac acgttgtttt
     3961 ttgagatatt ctaagttagg atgttattaa tgggcaactt atcaggaaac gttttcttat
     4021 gtttttgtcc aatttttttg tgaggtgttt attcttgaaa aaaataaaga aggagagtta
     4081 tggcttcctg acaatctttg tcgcttgtat aatgatggtt ttacagcgta tattgatttc
     4141 agcagtcact attcctacat atgcaacaaa ccctggtcga ttctacatct atacattttt
     4201 acaaacttcc gttgttattg gtgcaaattt gatcccactt tacatgggtt accaatatca
     4261 aaaaatcgcc cgtttaaaag ttttacatta tttaagtcgt tttgccttta tattcctaat
     4321 agccacacta gtttgcaata tcttttttta cgtacaagct ggcagcttga atattcgtga
     4381 ttattggctt atcctaacac ctatcagtca gaattacttt acttatgctg tttcttgtac
     4441 tttgcttttt tgtagtattc cttatacagt tggattttta aataaattat cgaaaacaac
     4501 aattaaatat ctactattat ttttaacaat tggattagtt ggctgctcta ctctatttaa
     4561 taaagacccg tttgcattac agaatggaca gagtatttta tggatttttt atttattttt
     4621 aatgggttat ggtttgaaag aatggaactg gaaagaaaag gtccgctata agcttcctca
     4681 agtatttatc agcttgattg ttttattcgg tttgattatt ttgatgacac aaatttcact
     4741 catcatcaga ggaaatacag atacagcgct gaggttttct aaaccatatt ctctcttcgc
     4801 aatgtattat actttctctt cgtttttatt tttagaaatg ctatctgata aattaggtat
     4861 aaaaggaaga ggaaattttc ttgctgtatt tttgattgtc actcaagtta tatgtaattt
     4921 ccctttagtt tcttatcgcg tatct
//
LOCUS       DAZULY010000191         4935 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000191 DAZULY010000000
VERSION     DAZULY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.193"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..601
                     /locus_tag="ACG81A_002293"
     CDS             <1..601
                     /locus_tag="ACG81A_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015619.1"
                     /translation="KSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLLTVAMFLLP
                     EKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKTYQDMLDVF
                     NDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRITAGKGGIT
                     DFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1051..2100
                     /gene="trpX"
                     /locus_tag="ACG81A_002294"
     CDS             1051..2100
                     /gene="trpX"
                     /locus_tag="ACG81A_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS5015620.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2097..2978
                     /locus_tag="ACG81A_002295"
     CDS             2097..2978
                     /locus_tag="ACG81A_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5015621.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            2981..3751
                     /locus_tag="ACG81A_002296"
     CDS             2981..3751
                     /locus_tag="ACG81A_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5015622.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4030..4884
                     /gene="aroE"
                     /locus_tag="ACG81A_002297"
     CDS             4030..4884
                     /gene="aroE"
                     /locus_tag="ACG81A_002297"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFS5015623.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
BASE COUNT         1656 a          859 c         1060 g         1360 t
ORIGIN      
        1 taaatcgaga gtacaagaaa tgaatgaaca tttctcagtg tcattcgcag aaaatccaag
       61 taagtatttt cgacgtcttg tttttctgct catcttatca ttgctgttaa cagttgcaat
      121 gtttttattg ccggaaaaaa tccgagaaga gcaagtaaat agtctcgttt attcgtccat
      181 accaaccagc aaattgaaac cttttgatta cagcaaagct gatcagaaaa tcaaacaaag
      241 ccgggcgata tcggtgatgt tcagcaaacc gaacgggaaa acctatcagg acatgcttga
      301 tgtttttaat gatcctgagc ttatggaaga attgaatcga ccgatctttt attatccgat
      361 cgtttatgat gtacatgaac tggaacaaaa atataacata cgaacggatg aagtgacatt
      421 tattttcttt gatggaggaa aagaagcaaa tcggataact gcgggtaagg gaggaataac
      481 tgattttgat aaggagttga tccctgaact gaatcgactt ccgttagcca atatcaaaca
      541 attagaagaa gaattggata gaacgggagt aacttcccaa aattccgaga ttgctaattg
      601 agacattggc gcgatgaaac ggtgggtcta tatagcgcta tttttaatta tcagaacaag
      661 tgatgtttga tcttcaaaat aacaaataaa aaattaaaaa atatagttga cgaaattcaa
      721 aaattcgatt agacttaaat caattcttaa ggaaagggtg acctgtaatg aacttatcaa
      781 aacgtatttc agcagaaaac aaaaaaaatt ggcgcagctg gcgtagataa gttgtattaa
      841 tggtgagact ttcccataga tacaactttt tagagtgcat ttctaaagct tgtatctatg
      901 ctagacatga acattgactg ccgcatagat tagaatctat gcggtttttt gtgtaaagac
      961 aatgaccgct ttggccattg tctttttttg tagactggat acttgcctga ttataataaa
     1021 taaaaagatt gactttgcag gaggaaaaaa atgaaaaaca aacgattaat aacagttgta
     1081 gtaattattt tagtttattt agcaggtgca tttatttatg gaaaagcctc aggcgaaaaa
     1141 gaaagtactt cgacgaacca gacaaaaaaa acaacagttg ttggggtatt gcaatacgtg
     1201 agccatccgg cattagatga gatttatcga ggaatcaaag atggtctgaa agaagaagga
     1261 ttaaaagaag gagaaaatct tgagatcaaa tttcaaaatg gacaagccga tcagagcaag
     1321 ctagccacaa tgagtcaaca gctagtacaa tctgatccag atgccttgat tggaatcgca
     1381 acacctgccg ctcaatcatt agcaaacacg acttcatcga tcccagttgt gttaggtgct
     1441 gtaacggatc ctgtgggggc tggattagtc gcttctttag agaaacctgg tggtaatgta
     1501 acaggtgttt ctgatcagcc gcctgttact tcacaaatca agcttgcttc tgatttgttg
     1561 ccaaatgcaa aaaaagtcgg aatcttgtat gcttcttcag aggataattc aaaataccaa
     1621 gtagcacaag cagaagaagc tgcaaaaaaa gaagggttag aatcagtaaa atatgcggtg
     1681 ccttccacta atgagattgc acaaacggtt caagtgatga gccgacaagt cgattttatc
     1741 tatgttccat tagataatac cattgccaat gcgatgcaaa cagtggtaaa agaagcaaac
     1801 aaagcaaata tccccgtcat tccatctgtg gatacaatgg tggaacaagg tggactagca
     1861 acaatcggga tcaatcaata tcaattaggg cttcaaagcg gtaagatggc ggcagaactt
     1921 gcgagtggaa aagaaaaacc ggaaacgacg cctgtttata catttgatca aggggataca
     1981 gtgatcaatc aatctcaagc tgatcgctta ggaatcattg ttccccaatc gatgaaggaa
     2041 aaagccaaaa tcattacaga tgactcgcag caagaaacat ctaaggagga tgacaaatga
     2101 tcgtatcagc tatttcacaa ggcatgcttt gggcaatatt aggattaggg attttcatga
     2161 cttatcggat cttagacttt cctgatatga cgacagaagg gtccttccca ttaggtgctg
     2221 ctgtctgtgt gacagctatt actcatgggg tagcgccgat tgtagctaca ttattaggcg
     2281 taggtgctgg catgatagca ggactcgtta cggggttact ttatacaaaa ggaaagatac
     2341 cggttatttt agcagggatt ttagtgatgt caggattgaa ctcagttatt ttgtttgtga
     2401 tgcgaacacc aaatctttct ttattgaatc atccgattct acaagacagc ttccatcgct
     2461 ttgggttacc tgattacttt gacacagtcc ttcttggctt catctctttg ggcatcttga
     2521 ttgctgtact gctattcttc ttcaatacag atttgggtca aggctatatt gcgacaggag
     2581 acaacgaaac gatggctcgg tcgttaggga taaaaaccga tcgtatgaaa attttaggtt
     2641 taactttgtc gaacggtgtc attgcattat ccggtgcgct gattgcccag aacgacggat
     2701 atgcagatgt aaataaaggg attggcgtaa tcgtcatcgg tcttgcttcc atcatcatcg
     2761 gtgaagtgat cttccaagaa ttgacgctag cagaaagatt gattgctatt gttgtcggca
     2821 gtatcattta tcagctattg attttagtag taatcaaact agggtttgac acgacttatc
     2881 tgaagttgtt ctccgctatc atcttagcta tttgtctgat gatcccgcaa ttgaagaaag
     2941 cactgaactt aaaaacgggt tttgagaagg aggcatagta atgactgata tattgacaat
     3001 caaaaatggc agaaaagtcg taaacaatgg aatgaatgaa gagaaagtac tattggatga
     3061 aattaacctt acaatcagag aaggggactt catcactgtc cttggcggga atggtgctgg
     3121 aaaaagcaca ctgttcaata cgatcgctgg tacgcttcaa ctcagtcaag gatcgatctc
     3181 ttttaaagat cagctgatca caaaagattc agaagaaaaa agagcacgat tcctttctcg
     3241 agttttccaa gaccccaaga tgggaacggc tcctcggatg acagtagcag aaaatttgct
     3301 acttgcacaa aaaagaggtc aaaaacggaa attccgttta cgtaacttga aaaatcaaaa
     3361 agaagatttt ttcagactat gtcaagaagt cggtaatggc ctagagcaac acttggatac
     3421 tccagcgggt gaattatctg gtggtcaacg ccaagcatta agcttgctga tggcgacgat
     3481 cacagctcct gagttactat tattagatga acatactgca gctttagatc ctaaaacagc
     3541 taaagcatta atgcgattaa caaatcagcg gatcaatgaa caaaaattga cttgtctcat
     3601 gatcacgcat cgaatggaag acgccttgaa atacggaaat cgtctgatcg tactccaaaa
     3661 agggaaaatc gttcaagata tttctgaaaa agaaaagcaa catttgacaa tgggcgacct
     3721 tttgcagttc tttgaagaag tagtggattg atttcgacag aaaaattcct ttcagtagaa
     3781 ggacttgaaa actcattgac atcttccttt gattcatcct atacttaaaa caataaacaa
     3841 ttaaacatat ttgggtgttt tgtaggatca cgtagtcaat gattttaaca attaaataga
     3901 atattctttg ctctgacaag taaacgatga gaaaagaatt tgctactttg ccacctagac
     3961 gtttcgtact ttttcgtatc aaacgctaag tggctttttt tattgaagta agaacaaagg
     4021 aggaataaga tgatttctgg acaaaccaaa ttagctgggc tctttgctga tccggcttct
     4081 cacagtttat ccccattgat gcacaatact gcttttgaag caaacaacat cgatgcggtg
     4141 tatttagctt tccaagtaaa tccaatgaat ttgaagcaag cagtcgaaag tattcgaact
     4201 tttgatatgt taggtgtaaa tctttcgatg ccgaataaaa cagcggttat tccttatctg
     4261 gatgaggtaa gccaggaagc gcaattgatc ggagcagtga atacgatcgt gcacgaaaag
     4321 ggtcgtttga ttggctataa taccgatggt gcgggcttca tgcaatccgt gcaagaagct
     4381 gggatttcga tcaaaaagaa aaaaatgact ctactgggag ctggaggtgc agcaaaggca
     4441 gtcactgttc aagctgcgtt agacggcgct gaggaaatcg tagtttataa acgaaacaac
     4501 caaacattcg ctcaagtaaa agaaacattc gaacaactca gctatcgaac ttcttgtaag
     4561 ataaaagttc gtgactttgc cgatacgcat caattaagaa aagacttgaa agaaagctgt
     4621 cttctagtca atgcaacaga tatcgggatg ggaaagaaaa tcaatcagtc accgctctta
     4681 gatataagca tgatgcatga gaatctagcg gtttttgatt tgatttatgc acctagagaa
     4741 acacgtttat taaaagaagc aaaaaaagta ggagcaaaag gatacaatgg acttggcatg
     4801 ttgatctacc aaggagcaat cgcgtttgaa ttatggacaa aggaaaagat gccgatccat
     4861 gtgattgaaa agctgtttga ttaaaaaagg ataatagata aagatctttt attctcgcat
     4921 aaacggtctc aagaa
//
LOCUS       DAZULY010000192         4842 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000192 DAZULY010000000
VERSION     DAZULY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.194"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(182..1144)
                     /locus_tag="ACG81A_002298"
     CDS             complement(182..1144)
                     /locus_tag="ACG81A_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5015624.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1146..2585)
                     /locus_tag="ACG81A_002299"
     CDS             complement(1146..2585)
                     /locus_tag="ACG81A_002299"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFS5015625.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2770..4716
                     /locus_tag="ACG81A_002300"
     CDS             2770..4716
                     /locus_tag="ACG81A_002300"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFS5015626.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1551 a          819 c          791 g         1681 t
ORIGIN      
        1 acagttgtgt aaaaatatca ttttagagca tgaaaaaagg agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacaag ttgacaactc ctttttgttt acaaatcaat
      121 tggatatcta caaattgtat tttctaacga attttataga cattaaaaaa ttaatttaac
      181 gttaaatact tcctccagaa agtattgaga ttggtaaaat ataatcctta tttgtctttt
      241 cgcctttaat ttttttcaga agaatatcaa caataagact tgcaatttca tcaattggtt
      301 gtcttattgt tgtcaattgt gggaaaaatt tctcaataaa agaggtacca tcgtatccaa
      361 taatttttaa atcttctggg atattaagtt taagttgatg agcaattttc atagtgagaa
      421 tggctgttaa atcatcagaa acaaaaatac catctggttt gtgatttata atagttgact
      481 ttatttccat ttcacgacgg atagtagaca gattattagg aattttgaaa atttttccat
      541 ttggattttg aaaagaaaaa cctagtgcac gtaattctgt gggagaatcc gtgttatcat
      601 ttcctgtaat cataatggta ttttgacaac catttttttg taaagtctta gctgcaagtt
      661 ttcccccttc aaagttatct gaagaaacaa tggaaacatt aggagctaaa tttctatcaa
      721 aagcaacaat aggtgctctc acacgctcat aatcagcaat tccaagatta tggcttgatg
      781 agattatgcc atcaacttgg ttggcttcaa gcatttcaat gtattctctc tctttagcag
      841 gatcattttg actattgcaa ataatcgttt tatatccatg tttaaataac tctatttcga
      901 gatactcaat gagttcagag taaaagatat tactaatatt aggaaaaata agaccaataa
      961 gctgaggtga ctttccttga agtccacgtg ctaaattatt tggtttataa ccaagagttt
     1021 tcatagcttc atgaactttt tgttttgttt tttctgataa atatccctta ttattaatga
     1081 cgcgtgaaac ggttgttggg ctaactccag ctaattcagc gacatcagtt aattttgcta
     1141 ccattttaat attttaattc aaagtatttt ccagtaggtt cgcctgattt gatttgaata
     1201 ccattttggt gattttcagg gaatacacga ctagtaaaca ctttttctcc tttattaata
     1261 aaaatctcaa tgattgagtt atctacaaaa atgttagcag ttgtttcttt agtatcaatt
     1321 gagcattcac gaatcgtacc aaagtcagtg gcatattgaa tgcctgcttt gctgcgatct
     1381 aaaataattt ttccttcttt ggtatctaca atcaagctaa gtccatttcc tttactatca
     1441 gagaaaagaa gtaactccga tttctgattt ggaggaaaag ttagttcaag ctcataagta
     1501 ttatttgttt ctgcttttgc tgtaaattgt tcagaactag cacgtaatga tgtaatagct
     1561 tctactggat attggtaaag tttaccatct tttattgaaa gttcttttac aagactcatt
     1621 gctccctgat aatcatactt atcagttgga taatcgatat caggtagtcc aatccaactg
     1681 acaatcagag cacgaccatc aggagtattg aatccttgag tcgcatacgc ttcaaatcca
     1741 taatcaagat tttgaatttc tgaagcgccc acaagtttta ctttttctat gtcaagcgat
     1801 tgacagatct taaaagtatt cggatagata ttcccataat ttagctcaga tttatccaac
     1861 ccttgaggac aataaatgag aacaggtttt ttgtctacaa atactagatt tggacactca
     1921 atcatgtatt ctgaaccagt gccgccaaaa tcaaggttgc caacttcctc ccatgtttcc
     1981 acgttattgt catccgcttt gtaaagtttg ataaagcctg atttttctaa attttgtgct
     2041 ccaacaatcg catacaattg tccttcataa ttaaacattt gtggatcacg aaaatgatca
     2101 gttacatcag caggtttatg aataagaaca ttctcacctt tcgtaatgtt gtagtcttta
     2161 tccatccatg ctccgatttg aagtgtatca cgttcccaat tttcatttcg aacgtttcct
     2221 gtatagagta agaatagatt cccctctatt tcatatgcag aaccggaata agcgccgtgg
     2281 ctatctcgtt ttgtgtctgg aagaagttct gctcctgtta catgaaaatg aactaaatca
     2341 tctgattcat tgtgtaccca ttctttcaac ccatgagcag ctccaaatgg ccaattttga
     2401 taaaaaagcg tatattttcc gttaaaaaaa gaaaaaccat taggatcgtt taacaatcct
     2461 gttttaggtt cgatatgata gctagcacgc caaggtgatc gttttacatt atccttaatt
     2521 ttatccatct catctttagt ccaatcagaa taagcacgat atcgtatttc tcgaggtaag
     2581 ttcatatttt actccttcaa tttatctttt tacaactagt atggtaaacg ttttccgttg
     2641 aaaaatcaat catttcattt aaaaaaataa actatatgac aaacgtttga catattttta
     2701 agatatgcta aaataaattt gcatttcagt gaaccgtttt cacgtaaaaa taaaaaagga
     2761 gtgattcgaa tgaattacag taaagtagct tccgacgtaa tacaagcggt cggaaaagat
     2821 aaccttattg cagctgcaca ctgcgctaca cgtcttcgtc tcgtactaaa agatgacacg
     2881 aaagtgaatc aaaaagcttt agacgaaaat ccagatgtga aaagcacatt taaaattaat
     2941 ggacaatatc aagtgattat cggtgcaggt gatgttaatt tcgtttatga cgaacttatc
     3001 aaaaaaactg gactttcgga aatttcaaca gatgatttaa aacaaattgc tagtaaagat
     3061 aaaaaattca accccattat ggcattaatt aaattattat cagatatttt tgttccaatt
     3121 atacctgccc ttgttgcagg tggtttatta atggcattac aaaacttttt aacagctgct
     3181 ggactttttg gaccaaaatc aattgaagaa atgtatcctg cgattgaagg tatttcctca
     3241 atgatccaac tgatgtccgc tgcaccattc atgttcttac caattttagt aggtatttca
     3301 gctgctaaac gttttggagc aaaccaattt cttggtgctg caattggtat gatcatgaca
     3361 acaccagatc ttggtggctc aactgaatat tggaatattt ttggttatca tgtttcacaa
     3421 acaaactatg cttatcaagt tattccagtt ttagcagctg tttggatatt atctatatta
     3481 gaaaaattct ttcataaaaa attgccatca tctattgact ttacattcac gccattatta
     3541 tcagtaatga ttactggatt tttgactttc acagttattg gccctgtaat gttattgttg
     3601 tctaatggaa ttacagacat tattgtttgg ctatataaca ctactggatt tattggaatg
     3661 ggtatttttg gaggaacata ttcacttata gtcatgactg gtttacatca atcatttcca
     3721 gctattgaaa ctcaattact ttcagcgtgg acaaatggta tcggacatgg agactttatc
     3781 tttgtagttg catctatggc aaacgttgct caaggtgctg caacatttgc catttggttc
     3841 ttaactaaaa attctaagac aaaaagtctt gcttcctctg cgggtgtctc agcacttctt
     3901 ggaattacag agcctgcctt gttcggagtt aacttaaaat accgctttcc attcttttgt
     3961 gctttaattg gttcaggaat tgctacagca gtagcaggat tacttaaagt agttgctgtt
     4021 tcacttggtt cagcaggttt cttgggattt ctttcaatta atgcaacatc tattccattt
     4081 tacgtattgt gtgaattgat tagctttgta gttgcttttg caattactta cttctacggt
     4141 aaaacaaaag ctgctggtat ctttaaagca gaagaaattg ctgaacaaac accagttaat
     4201 tcttcaaaag tagcaacaac agatccaata tctactgatg ttgaatcaac acaaacaaaa
     4261 gtttcatcag aaacaattgt tagtcccatt tctggtaaag tgattagtct ttcttcagta
     4321 aatgatccag tcttttcatc agaagctatg ggaaaaggga ttgccattaa accaaatgga
     4381 aacacggtcc attctccagt agatggtact attcaactca cgtttgaaac tggacatgct
     4441 tatggtatta aatcaaataa tggtgctgaa gttctaattc atattggtat tgatactgta
     4501 tcaatgggag gaaaaggatt tacacaaaaa gttactacta atcaaaaagt tagaaaaggc
     4561 gatattcttg gagcatttga ccgttcaatg attgagaaag aaggtcttga tgatacaaca
     4621 atgattattg tgaccaatac ttcggattat tcagaagtaa atatagtagc tgaaaataca
     4681 gtcactgagg gtactgattt acttgaaatc aaataaatat atttccctga gtaaagatgg
     4741 aattttcttt ccaaaatcaa ataatataga ttaatcaaat atttgagaat aacattttat
     4801 ttaatctttt catatatcaa ttgtatgaaa aattattaga at
//
LOCUS       DAZULY010000193         4838 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000193 DAZULY010000000
VERSION     DAZULY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.195"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..674
                     /locus_tag="ACG81A_002301"
     CDS             <1..674
                     /locus_tag="ACG81A_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFS5015627.1"
                     /translation="SLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQGMLELL
                     GTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGIFRMIF
                     AAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCWFFGQV
                     LFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKLAKKYY
                     PGKGQ"
     gene            676..1620
                     /locus_tag="ACG81A_002302"
     CDS             676..1620
                     /locus_tag="ACG81A_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFS5015628.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1819..2463
                     /gene="pcp"
                     /locus_tag="ACG81A_002303"
     CDS             1819..2463
                     /gene="pcp"
                     /locus_tag="ACG81A_002303"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFS5015629.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2542..3819
                     /locus_tag="ACG81A_002304"
     CDS             2542..3819
                     /locus_tag="ACG81A_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015630.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(4003..4752)
                     /gene="pnuC"
                     /locus_tag="ACG81A_002305"
     CDS             complement(4003..4752)
                     /gene="pnuC"
                     /locus_tag="ACG81A_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFS5015631.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         1555 a          862 c         1044 g         1377 t
ORIGIN      
        1 ttagcctttt aggcgtcttg attgttattc tcgggtttgc atttaaatta gactcgatat
       61 tgatcgtaat ggttgcttta gttgttacag ctttgactgg cggtcttggc attcaaggaa
      121 tgttagaact tttaggtaca agttttgtaa acaatcgcgg gatggcgatt tttatcatca
      181 ttatgttggc aacaggaacg cttgaaagaa atggtctgaa agaatcagcg gcttccttga
      241 tcaagcgatt caagaaagta tctgctggta tgattgtaga tatttatggg atctttcgaa
      301 tgatctttgc ggcttttaac gtgagttttg gtggagttgc tggatttgtt cgtccaatca
      361 ttttaccgat ggcattgggg accatcgaat caaaagggtt agaagtccat cctaagcatg
      421 aagaagaact aaaaggtatg gcttcagcta tggaaaatgt ctgctggttc tttggccaag
      481 tactattcat cggaggagca ggggcgttgc tagtccaatc aacactgaaa gatctaggat
      541 atgacgtaac attgggacag cttgctttag tggaagttcc agtggctatc acggctttag
      601 ttgtggcaag tgtctacttc tatcttaaag aaaagaaatt agcgaagaaa tattatccag
      661 gaaaggggca ataagatgga cttttttaca aatcctgata ttttattagg cgataaatta
      721 ctggagatcc tttatatcgt gatgggactc gtattgattt acactggagt aagaaattta
      781 acagataaaa ccaatcctca tcgttatggg tcagcctttt tctggacggt cttaggggta
      841 gttattgcag gaggaagatg gcttccggca atcgtgaacg gtggcttgat ctttgcgatg
      901 accataccag caattgctaa gaaagtcagc aaaggagaaa gccggcttcc atcaaaagaa
      961 tatatggaaa aaatgtcaga caaactagga atgaaaattt ttattccagc tttgagtatc
     1021 ggtgtctttg cgatcttgtt tgctctattt accaatttag gtgctttagt cggcgtgggc
     1081 gtcggtgttt ttgcagccat gctaatcttg atgttctttt ctcgagataa tcgaccaagt
     1141 acatttttag atgatgcagc agatatgctg ggcacagtag ggccgttaag catgttgcca
     1201 atgcttttag cttctctagg tgcggtattt acaagcgctg gagttgggac agtgatttcc
     1261 aatgcagtag gtacgatcgt tccagaagga aatgtgaaca tcggcatcat catttacgct
     1321 cttggcatgg tgatatttac ggtaatcatg gggaatgcat ttgcggccat caccgtgatg
     1381 acggttggga tcggtgcacc attcgtattt agtcatggag cgaatccagc gttaatagga
     1441 atggttgcat tgacttgcgg tttttgtggg accctgctga caccgatggc tgctaacttc
     1501 aatatcgtgc ctgtagcgat gctggagatg aaagacaaat acggagtgat caaaaaccag
     1561 ttgtttattg cgttatttat gctagttttc caaattgctt atatgattct atttaaataa
     1621 tagaagagag gggttgtgac agaataattg tcgcaacctc ttctaacatt gtagtggaac
     1681 actatttttt tataagaata tattaagttt gatctgttat gttatttaaa ctattgtttc
     1741 ctttatttac gctgttcaag gaaaatggta tgataaaaga taaccatatg ttaaatcaat
     1801 cggacggagg aacaacagat gaaagtacta gtaacaggtt ttgatccatt tggtggggac
     1861 aaagtgaatc cagcctatga agcagtaaaa aaaatgccag atgaaatcag cggtgctgaa
     1921 atcatcaaag tggaagtacc gaccgtattc gaaaaaagca gtcaagtagt caaagaagcc
     1981 atccaacagc accaaccaga tgtcgtgatc tgtgtcggac aagcaggtgg gcggtcagca
     2041 gtctcgttcg aacgtgtcgc aatcaatcta gcagaagcaa gaatccctga taatgaagga
     2101 aatcagccat tcgatacggc tttagaagaa aacggtccag ccgcttactt tacctcattg
     2161 ccaatcaaag caatgacaaa aaatgtacaa gatcatggtc taccagcgta catctcttat
     2221 acagcaggaa catttgtctg caatgatatc atgtatcgtt tgcttcattt gatcgaaaca
     2281 caattcccgg caattcgagg tgggttcatc cacgtgccat tctcacccga tcaagtgatc
     2341 gatcgtccag tgggtacgcc ttctatgtct ttagaagaca ttgcttcttc tttgacttat
     2401 gcagttgaag cagcagtaga aaataaagaa gatatttccg gaaatgccgg aacaactcac
     2461 taatttagca agaaacactg gctggttgct aacgttatgt tagtagccag ctttttgtaa
     2521 ttagaaagga aagaaacagc cgtggaagaa tggcaatccg tctttgaaga atggtttcca
     2581 aaagaaatca gcaaaagcta tcccatcaaa atttctaaac aatacacaag cagtcaacgt
     2641 tgggagatct atgcaaaatt aacgaaaaaa cagcgggaac tagtcgataa gcaccgccga
     2701 tatctaatca gctctcgttt tatggaagaa cattatttag ctgcaacaga ttgggtgttt
     2761 tctgacttca agatcaatcc gttttttcga acaaaacgaa gccagcaaaa attgtattgt
     2821 gagtgtggta gagaattaaa agtgcaatac atcgttaaat cgccaaaaac cggaaagatc
     2881 ctaaaactag gcatcaacca ctttgccgat cacctgcatg tctctccgac agtggcagct
     2941 tcgatccatc agggaatgac aaaagttgat ctggccttag acgaattatt atggcttaag
     3001 cagaagaata tcgattttcc tgaaggacta tggcaaaaat actgttttgt cttgtaccaa
     3061 aatcgtcgga tgaaacagcc atatttaccg gatataaaat tagctcagcg gctcgcagaa
     3121 tttcgccaag tagaaatgcc gatttacata gcggattatc aagcattgga aaatgaaatc
     3181 aagaaaataa gtgaacacat caatgggcag tcaaaaaagc gacagataaa aaaagaacta
     3241 tttgacgact ttgcagaaga attagtaaag gatgtagaag aattcttgat caactatcgc
     3301 gcatttttac gtaaagattg gcaatccatc gtatatgaag aagtgccagt acatcctaat
     3361 gcttattttg aaacatttat ttccgtgttg agaaagacaa aacgtcaacg aacaccagaa
     3421 gtcactgctc agatggaata ttttgcgaaa aaccaacgat tcatccaacc aaaaatctat
     3481 ctatttattt ggaagcaata ttgtcgctat ggttttacag aagggttctt cgacagcatt
     3541 cctcggatag tcagaaatgg ttttttaaaa gtattgcgta aagaacgaga agctatacaa
     3601 tctgctgata agaaagaccg tacagtctca aaagaaaaat ggcaactagt agtgaaagat
     3661 atccaatctg gaaatgtgca agagacaatc gacaaatgga aaggaaaaca ttatcgtttt
     3721 actgaagctc agaaacaagc gttggaatat tatcagaagt tagaagaatc actgcgtttc
     3781 aatgatgagg caagaaagta tctgaaggaa ttactataaa aaatagaggc tgggacagaa
     3841 gcgtttaact ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa
     3901 tttcagctta tttccgaagg agttgcttct gattccgccg tttatacgtg tttagagcgt
     3961 gagacaaaag tgttctttac ttttgtccca cgctcagttt ttttatgcgt gttgttgtgc
     4021 gttgtagtat tcttcttcca ttttttgcat acgttcacgt tctttttggt tataccaagg
     4081 tgaaacggta aatgcaagga catcattcag caagtaaaca gagctgttca cgaacatcgc
     4141 caaacttgca gatccatgcg taaatgaaat ataccaaagg accatttgag caagccctga
     4201 agctaaccac cagtaatatt ggttgttgta acgcaagaaa ctgatgatac cagcagtcaa
     4261 gctaatagaa aaagcaatgg catctaccca cggccgagga tcatctgtat aacgaccaat
     4321 caggaatcct gaaacaagat agacaaggaa agtactgatg attgcgataa accagctttt
     4381 tccagtgaat tttcggattt ttgaagccat attatgattc cattctttac ttaacaatac
     4441 ggggatatcc aatgtgatca tataagcaat ctgttcaccg atacttaaat agttctttgc
     4501 tgaaaaccca acgataataa aacaaatcgc agacaaaata cctaaaatac cgttgactga
     4561 ttttgccgca ttgatggata aaatacacaa tacaccaagc gtggtaccga taaatgtaat
     4621 aacggacagc caagtgatgg gactattgac aagagtcatg acctggcagc caagactgaa
     4681 aaagaaaaga taataatttt gttgcggcca gccttttagc tgatgagcta aaaactgaaa
     4741 atatgacttc atgtatgagg actccttaag acactaaatg tagtgtgttc tgtatttact
     4801 aacacaatct attgtatgct tttcttgaaa aaaaacta
//
LOCUS       DAZULY010000194         4826 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000194 DAZULY010000000
VERSION     DAZULY010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.196"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1662)
                     /locus_tag="ACG81A_002306"
     CDS             complement(52..1662)
                     /locus_tag="ACG81A_002306"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFS5015632.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(1884..2129)
                     /locus_tag="ACG81A_002307"
     CDS             complement(1884..2129)
                     /locus_tag="ACG81A_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015633.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            complement(2855..4195)
                     /gene="glnA"
                     /locus_tag="ACG81A_002308"
     CDS             complement(2855..4195)
                     /gene="glnA"
                     /locus_tag="ACG81A_002308"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFS5015634.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4223..4615)
                     /locus_tag="ACG81A_002309"
     CDS             complement(4223..4615)
                     /locus_tag="ACG81A_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFS5015635.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1410 a         1044 c          791 g         1581 t
ORIGIN      
        1 attgcttatc cccttctctt gctacttttc tctttctatc aagttcaaaa attattttgt
       61 ttcgtgatct gctaatgatg ctccgacaaa tgctttgatt aatttttgcg ggcgatttgg
      121 acgagaaatc aattctgggt ggaattggca tcccacaaag aattttttct ctggcagctc
      181 tacgatttct actaaacggt tatctggtga gacacctgaa aagactagcc cttgttcttc
      241 aaacaattga cgatatttgt tattgaactc atagcggtga cggtgacgtt cttgcacaac
      301 atcggctcca tcatatgctt cagccgtctt cgttcctttt ttgattttac atgggtataa
      361 tccaaggcgt aaagttccac ccaaattctc gatgttttcc tgatcagcca ttaaatcaat
      421 aatattgttt gctgtatctg gattcgtttc agctgatgac gcatcttcta atccaacgac
      481 gttacgtgca aattcgacac aagccatctg cataccaaga caaataccta aaaacggaac
      541 gtcattttcg cgagcataac gaatcgcttc gattttccct tctactccgc gatcaccaaa
      601 tccaccagga accaagattc catctgcgtc tttcaaacgt tcttctacat tttctctcgt
      661 taattcttgt gctttgatcc aatcgatctc gatgtctgaa ttataggcga aaccagaatg
      721 tttcaatgct tcaactactg agatatatgc atctggtaac tcgacatatt taccgacaag
      781 cgcaatccgt acttttttct tcaagttcaa gacacgttct tctaattctt tccattctgt
      841 catgtctgct tctggtgtat ctagttttaa atggtcgcaa acaatttgat ccattccttg
      901 tgcttgtaaa actaatggga tggagtaaag tgtatctaca tctggcgact cgataactgc
      961 ttctggttcg acatcacaaa attgtgccaa tttgtttttc acgctttgag aaacaggttg
     1021 ctctgtacgt accaccaaaa tattcggttg gatacctaaa ccacgtagct cttttacact
     1081 gtgctgcgtt ggtttagttt tcatttctcc agcagctttc aagtaaggaa tcaatgttgt
     1141 atggatatac ataacattat ctgtaccgac atctgctttc atttgacgaa gtgcttcaag
     1201 gaatggcaaa gattctatat cccctactgt tccccctact tctgtaatga tcacatctga
     1261 atcagtcatt ttagctgctc gcatgatttt ttctttgatc tcatttgtaa tatgaggaat
     1321 aacttgaaca gttgctccca agtattcgcc cttacgttct ttacgcaaaa cttcagaata
     1381 gatttttcct gtcgtcacgt tggagtattt gttcaaattg atgtcaataa aacgttcgta
     1441 gtggcccaga tccaaatctg tttcagcacc atcatctgtt acgaatactt ctccgtgctg
     1501 atatgggctc atcgtccccg gatccacgtt gatgtatgga tcaaattttt ggatagttac
     1561 ttttaagccg cggtttttca ataagcgtcc taaagaagct gcaacaatcc cttttccgat
     1621 tgatgaaacg acaccgccgg tcacaaaaat atattttgtc ataagttcaa aaactccttt
     1681 ttaatttatg tgcaggaaaa gtctcagaca ataaacaaag ctccctattc ttcaataaga
     1741 atagggagcc gaaatttctg taaacttctg accctctaaa agggtgccca aggtgtataa
     1801 tacaagctga ctaagggaaa gtcaagtact caaatgaata ctatccgctt acaatttttt
     1861 ctttttcgat atcctttgag aaattatttc ttaagtcctt tatgagtgca aaaaaaattt
     1921 aacccacata ataagcttga tataaacata gctaacgtta aacctaagca aatcagaaaa
     1981 tcgttactga aaaattttaa gtgaaataat agataaaaaa ataaaacaaa cattatgata
     2041 gataatatcc cacctaacaa agaactactt tttcctactt gcagcaacag tttatatagc
     2101 ttatcgtaga aacaatcttt ttctttcacc cttatttacc tcctctacgt aaaaaacgta
     2161 gtccacagtt ctcggatttt tggcatatct tttctattaa ataatatcaa aaacaaaaca
     2221 attccactaa ttttttcaaa gatgaccttt ttttcaacaa aagtgccttg tagggattca
     2281 ttgaaggtaa cgaatcacat aagtaaaaaa taaggagatg cgacaaaagt cgtgcctcct
     2341 tatttctaaa taaacggtgt tctcctgcca actcctcggt cacaagtcac aatatccatc
     2401 aaacatgaga agctgttttt gaaattattt cttgtgattc ggagttaaat ggtaggctca
     2461 caacctctgg ataaacggta ttcaaaagcc agtttctcgg tattaagcca aatttatgca
     2521 aaaatgtaga aagccatttt cacaaaattt ttcttaatac tcgaaactaa acgtcttttt
     2581 caaaacctct tggtaaacgg tgttcaaaag cttgcatctg cggtaataag ttcagccaat
     2641 tcaaaaacaa gaaaagctgt ttttgcttgg ctattcttat tacttgttgc agggcagctt
     2701 tttcacaacc tctccactaa acggtgttca atcagctgac cctcggtatt agctcaaaaa
     2761 acggcaaaat atgaggaact atttttccgt tagtttctct aatactcagg tcaaaacgct
     2821 gatttcacag cctctaattc acggtattcc tgttttaata aagttctaga tattgttctc
     2881 tttcccactc ggaaacagtt tgtctgaatg ccgcccattc gagacgtttt gcttcaacaa
     2941 aatttgagaa gatatgttgt cctaatgcgt caatcattac attatcctta cgcaattctt
     3001 tgatagcatt atggatagtt gaaggtaagt cctggatatg tgcagcttct ctttcttcct
     3061 catccattac ataaatgtta cggtcaacag ctgctggcgg tgtgatctga ttacggatac
     3121 cgtctagtcc tgcttgcaac aatactgcca ttgccaagta tgggtttgca gatggatcaa
     3181 ctgaccgcaa ttccaaacgt gtggataaac cgcgagattc tggtacacgt actaatggcg
     3241 aacgattacg gccactccat gcgacataaa caggcgcttc ataacctgga acgagacgtt
     3301 tataagaatt gactgttgga ttgcaaacgg ctgtgtaggc acgagcatgt ttcagcaaac
     3361 cacctaagaa atgataagct gtttcactta gttccaattc accgtcttta tcatagaaaa
     3421 catttccttc atcattgaac aaagacatat tgcaatgcat accagaacca ttgatcccaa
     3481 ataacggttt cggcatgaaa gtcgcgtgaa gtccatgctt gcgtgcaatc gttttgacaa
     3541 cgagcttgaa ggtttggata ttatcacaag catctaccac atctgcatat ttaaagtcaa
     3601 tttcatgctg tcctggcgct acttcatgat gtgaagcttc cacttcaaag cctagacttt
     3661 ctaattccaa cacgatgtca cggcggcaat tttcacctaa atctgttggt gcaaaatcaa
     3721 aataaccacc tttatcattc aaggtcgttg tgatcccacc gtcttcgtct aatttaaata
     3781 agaagaattc tggttcaggt cctaaattga aagaggtaaa accaaggtcg cgcatatctt
     3841 tcaatgctcg tttcaaattc ccgcgaggat cgccggcaaa tggtgtccca tccggattat
     3901 agatatcaca aattaaacga gcaacttttc catgagcact ttcccacggg aagatcatcc
     3961 aagtggagag atctggatac aaatacatat cgctttcttc aattcgaaca aatccttcaa
     4021 tcgaagaacc gtcaaacatc attttatttt ctaatacttt gtcgatctgg ctcactggaa
     4081 cttctacatt tttgatcgta cctaagatgt ctgtgaacat caatcgtaaa aatcgtacgt
     4141 tttcttcctc aatgattcgt ttgatttctt cacctgttat ctgtttcttt accatggtat
     4201 ccctcttcct tttttaattt attcagtcaa agaattgtct ttgcaatcct tgcgactgat
     4261 aatgacctgt tttgcttaac ccaccttgcg ccaaaatctc atcacgaaaa atttgtcgaa
     4321 cgtcatgatc tgtcaacggc ttcttgtttt ttgcttcttg ttttttcgcc tgtgccattt
     4381 cgtatacacg tttgatccca gccatattca atccctctga aagatagtct ttaatctcaa
     4441 gcaacatatc aatatcattc agcgaataca tacgtcggtt tccttcgctt cgttccggat
     4501 gaactaaatc ttgttcttca taatagcgaa tttgccgagc agacaaatca gtcagcttca
     4561 taactgtacc aattggaaat acggacatag atcggcgcaa ttccttttct cccatcagac
     4621 gtcccctttc gttttataag ttggctaaag tgtaccaatt aaaaaaaata ttgtcaactg
     4681 catatgttag gttttctaac atgagatctc tattttttat aaaataacaa aaaaataaga
     4741 acacaaaaaa ggtatctagt tgattttcgt accagatacc ttttattttt tattatactg
     4801 tactatcctt tattagtgtt tttatt
//
LOCUS       DAZULY010000195         4788 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000195 DAZULY010000000
VERSION     DAZULY010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.197"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            564..989
                     /gene="bacA"
                     /locus_tag="ACG81A_002310"
     CDS             564..989
                     /gene="bacA"
                     /locus_tag="ACG81A_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="HFS5015636.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="ACG81A_002311"
     CDS             1019..1183
                     /locus_tag="ACG81A_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015637.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="ACG81A_002312"
     CDS             2141..3118
                     /locus_tag="ACG81A_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS5015638.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="ACG81A_002313"
     CDS             3128..3625
                     /locus_tag="ACG81A_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFS5015639.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="ACG81A_002314"
     CDS             4001..4282
                     /locus_tag="ACG81A_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015640.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="ACG81A_002315"
     CDS             4284..4700
                     /locus_tag="ACG81A_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015641.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1758 a          817 c          785 g         1428 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtacacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DAZULY010000196         4778 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000196 DAZULY010000000
VERSION     DAZULY010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.198"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..258)
                     /locus_tag="ACG81A_002316"
     CDS             complement(<1..258)
                     /locus_tag="ACG81A_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015642.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="ACG81A_002317"
     CDS             complement(274..924)
                     /locus_tag="ACG81A_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015643.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="ACG81A_002318"
     CDS             complement(935..2107)
                     /locus_tag="ACG81A_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFS5015644.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="ACG81A_002319"
     CDS             complement(2126..2998)
                     /locus_tag="ACG81A_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFS5015645.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="ACG81A_002320"
     CDS             complement(3020..3940)
                     /locus_tag="ACG81A_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015646.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="ACG81A_002321"
     CDS             complement(3940..4209)
                     /locus_tag="ACG81A_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015647.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="ACG81A_002322"
     CDS             complement(4206..4586)
                     /locus_tag="ACG81A_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFS5015648.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
BASE COUNT         1252 a         1049 c          789 g         1688 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtaca
//
LOCUS       DAZULY010000197         4733 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000197 DAZULY010000000
VERSION     DAZULY010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.199"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(278..1351)
                     /locus_tag="ACG81A_002323"
     CDS             complement(278..1351)
                     /locus_tag="ACG81A_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5015649.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(1348..2175)
                     /locus_tag="ACG81A_002324"
     CDS             complement(1348..2175)
                     /locus_tag="ACG81A_002324"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5015650.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(2176..2982)
                     /locus_tag="ACG81A_002325"
     CDS             complement(2176..2982)
                     /locus_tag="ACG81A_002325"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5015651.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLLLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(2994..4079)
                     /locus_tag="ACG81A_002326"
     CDS             complement(2994..4079)
                     /locus_tag="ACG81A_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5015652.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(4096..4638)
                     /locus_tag="ACG81A_002327"
     CDS             complement(4096..4638)
                     /locus_tag="ACG81A_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS5015653.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
BASE COUNT         1447 a         1021 c          773 g         1492 t
ORIGIN      
        1 gattacagaa gggttaagta tatttgcata gtttccaatt actttatcct tctgtaactt
       61 tttcaatctt agatttacta gcccgtctcg ggccctattt tacaatggtt ttgtccactt
      121 ttctctggta gaaaaacgtg gtagaaaaaa taaaataaca caaaaaaagc ggcgtttcat
      181 ccgcttttgg tagaagattg gaaaactcag atagaaaaag gacgattttt agctctttag
      241 aacgttgata agtccccatt cttactgagt tttgccatta tctcctatac atcttgaatt
      301 ctaagaatag gtcattatag atgcctaggt atttcgatcc tagatctttg tagacttcaa
      361 gatgggagat cgtgtcatca gatggataga attgttcgtc cgacgaaatg tcttttggca
      421 ataacgcttt tgcttcttca tttggtgtag aataaccaat atactctgca ttttgtgccg
      481 cattttccgg ttctagcata aagttgatga aatcataggc accttcttta tttttcacgg
      541 tttttggcat aacgatatta tcaaaccaaa gattggatcc ttctggtggg atcacataat
      601 gtaaatgttc attttcccac atcatatcgg cagcttcgcc agaaaatgtc actgccacgg
      661 aggcttcttc attgatcata tacattttga tctcatctgc cacgattgct ttgacatttg
      721 gtgttaaatg attcaattta tttgctgctt ctgtcaactg tgccatattc ttactattca
      781 aagaatagcc taaactattc aaagaaagcc ccataacttc tcgtgcaccg tcaatcagca
      841 tcaaactgtc ttttaattcc ggcttccaca aatcatccca atgcttaatt tgatttgcat
      901 caaagaattt atcgttatat acgatcccta aagttcccca aaaatatgga atcgaatact
      961 gattatctgg atcaaaatcc aagtctaaaa atcgtggatc aatattattt aatcctttga
     1021 tcttcgaatg atctaatgga agtactagtt tttctttgat cattttttgg atcatatatt
     1081 cacttggtat agcgatgtcg tagtttgtcc caccttgctg gatttttgtg aacatcgctt
     1141 cattagaatc aaatgtttca taattcactt tgtatccata ttctttttca aatttcgtga
     1201 tcaaactagg atcgatataa tctccccagt tatagatggt aagtaccttt gctccactca
     1261 tgccacttgt tttttcaagt tgacgaacac caagcagcag caccgcaata atgatgagaa
     1321 tacctaaaaa cagagattgt aattttttca tcgcagccgt gccaccccct cgcgtactct
     1381 tctgtttttc ttcagccgtt ttggacggtt ttctttacta ataaagtagt atccaatgac
     1441 caatatcatg gagaacaaga aaactaacgc acttagcgca ttgatttcta aactgattcc
     1501 ttgtcttgca cgagaataga tctctactga gagcgtcgta aagccatttc ctgtgacgaa
     1561 aaatgtaacc gcaaaatcat caagagaata ggtaaatgcc ataaaatatc cagctatgat
     1621 tcctggagaa agataaggca aaatgatatt tttgactact tggaaattat tagcaccaag
     1681 gtctctagcc gcatcgacca ttgaatcgtt catttcctgc aattttggca agaccatcaa
     1741 gacgacgatt ggaatgctga aagcaatatg ggaaagcaat acactctgaa aacctaatcc
     1801 gaatcctagt agtgtaaaga aaatcaagaa actagcaccg ataataacat caggagagac
     1861 taataagatg ttattcatgc tcaacaacgc tgtccgtgca cgtcgttttt tcgtgtaata
     1921 gattcccata gccccaaatg tcccgatgat cgtggcgatc aatgcagata agaaagccag
     1981 catgaacgta ttcaatacga taatgatcaa acgtgtatct tcaaaaacag cttcataatt
     2041 ttcccaagta aatcctgtaa agctgttcat cgttcctcct tcattgaagg aataaaaaat
     2101 cagatagaag atcggtgcat ataagaataa aaagacgatc cctaaataaa gatacgacca
     2161 tttaaatttt ttcatttatg agcgacctcc ttttttcttc tcgcctgtca ggagcatcac
     2221 gatgaacata gcaatgatta aaatcacacc gattgtagaa cccatccccc agttttgtgt
     2281 aaccaagaaa tgctgttcga tagccgttcc taaagtgatc acccgatttc cgccaatcag
     2341 acgagtcagc atgaataaag aaagagaagg aataaatact gcttggacac cacttttcac
     2401 accatttaat aataacggaa aaatcactcg tcggaaggtc tcaaagctat tcgcgcctaa
     2461 atctctacta gcactgatca aagaaggatt catctcttcc aatgcattga aaattggcat
     2521 aatcataaat ggaatctcga tatacgttgc cacgaacaga aaactgaagt ctgtgaataa
     2581 tatctgtttc ggcccgatcc cgataaaatc aaagaaatga ttcacgctgc cgtgcacact
     2641 gaaaatccca ataaatgcgt aagctttaag cagcagattc acccatgtag gcaaaataat
     2701 cagcatcagc cataattgct tgtgcttcag ttttgttaaa aaataagcag tcgggtaact
     2761 gatgatcagc gtgaaaaacg tgatcaaaaa tgcataccat acagaattga tcgtcataga
     2821 aagcgatgtc cccgacgtaa aataagatag ataattattc agcgtgaatt gcccattcat
     2881 atcaaaaaaa gattgataaa tgatcattaa tacaggcgcg atcacgaata aaccgagcca
     2941 caaaatataa gggacggaat agatcctgcg cattgttttc attcttgtcc cccctattct
     3001 tcgtagctgt caagacgtgc atcgaattct tcttcagact cgttgaaacg catgacatgg
     3061 atatcttctg gctcaaaggc caatcctact ttgcttcctt ctttagcttt tttcgttgaa
     3121 tggaccatcc attcattttg ctgttcatca taacagatga tttcataatg gacaccacga
     3181 aacagctgag tatcgaccgt tacgactagt tttcctttat ctggtgtcgt gatcgtcaaa
     3241 tcctctgggc gtaaaacaac ttcaactggt tcatttagac gcatcccgcc atcgacacat
     3301 tcaaattgct taccaacaaa ctcaactaga ttatcttcga tcattacacc gtccacgata
     3361 ttgctctccc caacaaaatc agcaacgaaa tgattgattg gctcatcata aatatctacg
     3421 ggtgtaccac tttggacgat tttcccttta ttcatgacaa aaatctcatc actcattgcc
     3481 aacgcttctt cttggtcatg agtaacaaaa ataaacgtga ttcctaatcg ttgttgcaga
     3541 tcccgcagtt catactgcat atctgtacgt agtttcaaat caagcgccga taacggttca
     3601 tctaatagca atactttcgg ttcatttaca attgctcgtg caatcgctac acgctgtctt
     3661 tgaccaccag acatttcact aatctcccgt ttttcgtatc caggaagctg gaccatacgc
     3721 aaagcatctt ttaccttctt ttcgatatct gatttggaca ttttcttgat cttcaatcca
     3781 aaagcaacgt tatcaaaaac attcatatga ggaaaaagtg cataatcttg gaagactgta
     3841 tttacttgtc gcttattagc tggcaaatca ttcaaccgtt tcccttcaaa atagatgtcc
     3901 ccttctgtag cttcggtgaa tccagctata atacgtagga tcgttgtttt accacagccg
     3961 gaaggaccaa gcagtgtata aaattttcct tgttcgatct caaaacttac tttttttaat
     4021 actgtttcat catcatagcg tttcactact tgatcaaatg aaatgattgg tttccccacg
     4081 tttttacccc ctatattaca aatatgattg cgttgcaaca atcaatactt ttgttcgttt
     4141 tttgagatga ttccttaatt gatgttgttt tgtcgcttga taatagatcg actgtcctgc
     4201 ttttgccaca tagaccgtct ctcctaagct taactctaat tccccttcta atacaaaaat
     4261 aaacgtttca gataatgaag gttcaaacgt cttatattct cctgcacctt caaaagtcag
     4321 caagacaggc tccatttctt tttcattcga atcagccacg agccattcca attcataccc
     4381 gttttcttca tcgaaataaa ccgtatgatc ttcttttgaa taaaccactt gcagattcgc
     4441 tgcttcttga tgaaaaaatt cttctggagt cacacctaag acttcaagaa tagaaaagaa
     4501 tgtttccatt gatggtgaac ttaaatctcg ttccaactgg gaaatatacc cttttgacaa
     4561 atctgtgcgt tcgcctagtt cttcttgtgt caaattcttt tggatgcgca aatttcttaa
     4621 cttctcacca atttccataa tttcctccga atcgaagttt tctaatagta aacttcctgt
     4681 ttactaccac tcgtattagt atacgaaatt tattgaaaaa ggcaaccctt tgt
//
LOCUS       DAZULY010000198         4712 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000198 DAZULY010000000
VERSION     DAZULY010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.200"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..357
                     /locus_tag="ACG81A_002328"
                     /pseudo
     CDS             106..357
                     /locus_tag="ACG81A_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="ACG81A_002329"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="ACG81A_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFS5015654.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="ACG81A_002330"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="ACG81A_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            1786..1869
                     /locus_tag="ACG81A_002331"
     CDS             1786..1869
                     /locus_tag="ACG81A_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFS5015655.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="ACG81A_002332"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="ACG81A_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS5015656.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="ACG81A_002333"
     CDS             complement(2994..3857)
                     /locus_tag="ACG81A_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFS5015657.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="ACG81A_002334"
     CDS             complement(3859..4131)
                     /locus_tag="ACG81A_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS5015658.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4363)
                     /locus_tag="ACG81A_002335"
     CDS             complement(4148..4363)
                     /locus_tag="ACG81A_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFS5015659.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4712)
                     /locus_tag="ACG81A_002336"
     CDS             complement(4455..>4712)
                     /locus_tag="ACG81A_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HFS5015660.1"
                     /translation="MIGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKV
                     STWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1446 a          796 c          865 g         1605 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc at
//
LOCUS       DAZULY010000199         4673 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000199 DAZULY010000000
VERSION     DAZULY010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.201"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(224..835)
                     /locus_tag="ACG81A_002337"
     CDS             complement(224..835)
                     /locus_tag="ACG81A_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS5015661.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(958..1083)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1165..1881)
                     /locus_tag="ACG81A_002338"
     CDS             complement(1165..1881)
                     /locus_tag="ACG81A_002338"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFS5015662.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1887..2456)
                     /gene="scpB"
                     /locus_tag="ACG81A_002339"
     CDS             complement(1887..2456)
                     /gene="scpB"
                     /locus_tag="ACG81A_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFS5015663.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2440..3210)
                     /locus_tag="ACG81A_002340"
     CDS             complement(2440..3210)
                     /locus_tag="ACG81A_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFS5015664.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3207..3578)
                     /locus_tag="ACG81A_002341"
     CDS             complement(3207..3578)
                     /locus_tag="ACG81A_002341"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5015665.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3597..4484)
                     /gene="xerD"
                     /locus_tag="ACG81A_002342"
     CDS             complement(3597..4484)
                     /gene="xerD"
                     /locus_tag="ACG81A_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFS5015666.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         1283 a         1005 c          804 g         1581 t
ORIGIN      
        1 tttaaagctt tcttcttttt cattgtagcg tttccttttt tttataaaca aacattttcg
       61 tcttttaaaa ggagaagaat ttcacatttg caaagaattg attgattttt atgcataaat
      121 caaaaaaata ctcaaaacaa acaaataaaa cgtttacatt attgcatgtc ttgtattttt
      181 ttccaaaaaa aaagaccaag aaatttcttg atctttttga tatttatttg actgctgtat
      241 gacgttgttc gaactgatgt tgttttcgac ttaaccatgg aagaagttgc gcatgcagca
      301 ctaaaaagac agcactcacg atgaatcctt tgatcaagtt gaaaggaacg atcccaatca
      361 gtacatagtt tgctaaagac atgcccagca tctgtgttgc gttcagccct aaaaacatta
      421 agtaaagagg cgtaataacg aaataatttg cgatactcat aaaaatagtt agagcgatgg
      481 ttcctgtaat gacacctaaa aatttgtttc ttcctttatg ctgtttcttt acactaaaga
      541 aataataaat cgggaatgta aagatcgttg ttgataagaa actagcagtg tctcctacca
      601 aattatccgg agaaaaacct gtagtcagca gatgtaatac tgaacgcaag aacgctgttg
      661 caactccggc cgctggaccg aataagaaca tgctgatcaa taccgagata tcactaaaat
      721 cgatcttcaa aaaactaaaa gccggcatga tcggaaaagc aaaatactgt aaaactaatg
      781 cgatcgcagc aaacatcgct actgcaacca ttttctgtac acgggtgttc ttcatttgaa
      841 atcctcctgt taggactcct cttcagcgaa ctttttgacg tttctctcca aatgaaaaaa
      901 ctctattttc cagggaaaat agagttagag ttatttgatc aagcgtcgtc aaataagctc
      961 tatcttcttt atccagacta tactgtcggt atcggaattt caccgattca gctatttttt
     1021 tgcaaaatag gtcgtggact ataaccaccg gtcgggaatt tcaccctgcc cctgaagacg
     1081 aaccttatat aatttttgtt tacatattca ttatacatat ctattcaaaa aacgcaacat
     1141 ctaacaatta taaaaagtaa gtgttcattt tgagtggttc agcaaactgc tgacttcttt
     1201 tttgttcagt tcacggtaat ccccaggacg taggccttgt aaagtcagat ctccgtattt
     1261 ttcacgtttt agtttttgca caggaagacc aactgcttga agcatatttt tcacttgatg
     1321 attacgtcct tcgtggatcg tcaattccac gatactgtgg tttgtcttag gatccactga
     1381 taaaatttgg aatttagctg gagatgtttt tcgtccttcg atctttattc cttttgtcaa
     1441 aggtgcaagc atcgattttg tagcgatccc ttttaccttt gcaacgtaaa ctttatccac
     1501 ttcatgtttt ggatgtgtca ggcgctgagc aaaatctcca tcatttgtca gtagcaagat
     1561 ccccgaagta tcgtaatcca gtcgacctac aggatagata cgttctttta ccatcggcaa
     1621 aagatccgta acaacttttc ttcctttgtc atctgcaaca gcagaaataa ctcctttagg
     1681 tttgtacaat aaatagtaac cgtattcttc ttgataaatt gggacttcat ccacttccac
     1741 agtgtcatgt ttcccaactt ttatccctaa ttcccttaca acttgtccgt ttacttttac
     1801 acgtcctgca aggatgaatt cttctgcttt tcgacgagaa gtgatgccag cgtgggcaat
     1861 tactttctgc aatctctcca ttacaattat tctcctttat tcattccatc aaagaataga
     1921 tctggtgaca cgtcttcttc aagtgaaatt tccatttctt gtgtatcagg cagttcttct
     1981 aatgatttca aaccaaaata atctaagaag tagccagttg ttccatataa tatcggccga
     2041 cctggtcctt ctactctacc tttttcttca atcagtttat gggcagctag acgctgtacc
     2101 gctcctgaaa cctgaactcc tcgaatctgc tcgatttcag ctcttgtgat tggctgttta
     2161 taagcgatga ttgctaaggt ttcgacggcg gcttgagata atgtgtttga tgtcccttgc
     2221 gcatatcgct taagtaaggg agccagctct tttttagtcg ttaatacata atggctttcc
     2281 gtttcaaaaa tatgcaaagc tgagtgcgag ttttgagtgt aagattcttt taattctttc
     2341 actaaacttg tgacttctgc ttcgctgaga tttaacagtc cgcttaattc ttcattactg
     2401 atcccttcct cgccaacaat aaaaagaagg gcttcaagtt cacttaattt cgtcatgttc
     2461 ggtatctcct actgctggat aaagcataat tgattcatag ttttcttttt gttgcgcaat
     2521 gatctgccct tttttcatca attctagcaa agccataaaa gtagtgacaa cttcactttt
     2581 agaaggtgac tcaaaaaaag aatcgaaagt acagccatgc gcatttcttt ttatacgctc
     2641 ttcgataaat accattcgtt cttcgatcgt tgtgtcatcg ggcgtgatcg ttttttccac
     2701 tttttgagaa aatttcttac gttctaagat tttctgcatg gcacggatca aatcgatttt
     2761 tttgacttgt cctcgcttca acggcttttc agcatccgct aacccatcga tgttcatagg
     2821 ttcttttgtg taataaagac ttcgttcctc tgctttttct gtcagtcttt cagctgcata
     2881 tttatattta cggtattcta aaagctgagt gaccaattct tctcgaggat cttcttccca
     2941 taattcttca tcgtctgtgc cgaattcttg gacaggaagc aatgtcttgc ttttgattgc
     3001 catcaatgta gcagccatca ctaagtattc tcctgcaaca gctagctcga gtgttttcat
     3061 tgtatggata taattcatat attgctccgt gatcgtagcg atcggtatat catatatatc
     3121 gatttccatt tgctgaatca gatgaagcag cagatcgaga ggaccctcga atacatctaa
     3181 tttaatattg atctccgtca actcattcac tctttcttct ttttctttgc tagccatttt
     3241 ccaatgtaag gctctgtgat ttcagttgca cggatggaga catccgggta cttttcctgt
     3301 aattcatcta acatttgaaa gcccatattc tcaccgcgat aagacggatt caaagaaatg
     3361 tcatggatga tcagctgatc gttcgttgcc cagtaaatac caataatccc ttgaatgttg
     3421 gttgaacctt ctggcgtaaa gaaaaaaagt tcataatcgt cttcagattc gtacgtatca
     3481 atctccttca acaaagaagt tttacctgtc agtttcttat ggaaactcaa tagtcccata
     3541 gcaattttac ggttttcttt ccggtaatgt gttaacatct tctcaccttc ctatgactaa
     3601 gcccttggaa aatgctgctt ataaacatcc gtcattcttt tcttcgtgat atgtgtataa
     3661 atctgagtcg tagagatatc cgcgtgacct aacaattcct gtactgtccg caaatcagct
     3721 ccattttcta gcagatgtgt ggcaaagcta tgccgcagcg tatgtggtgt tacttctttg
     3781 taaatcccag cctctcgtac taactgcttc agatttttcc agattccttg ccgagaaaga
     3841 cctttcccat gattattgac aaatacatgc atttcagatg cgtcctttac aagtaatgga
     3901 cgtgcttcgt ctaagtagcg ttccagccat tggatcgcat aatcacctaa aggaatgatc
     3961 cgctctttat ctccttttcc aactgtctgt accagtccaa gagaaagatg aagatctccc
     4021 aatttcaatc caatcaattc actgacgcgc atcccagtag catacatgac ttccaagatt
     4081 gcacgatctc ttatccctaa cgtttttgtc gtatcaggtg cttcaatcag cttttctact
     4141 tctgtcagag tcaatgtact tggtaatttc tgtgctttct tcggcgtatc gatatgttgc
     4201 atcggatcat gatcagtgat acgctcttgc cgtaaaaact gatggaatct tctgagactt
     4261 gagatcatcc gagtaacagt tgctgtcgct tttttttctt catgcaactt ctccagaaaa
     4321 cggatgacta aaaagcgatc gacatcctgc catgaagcaa ttttctgctc ctgtagaaaa
     4381 tgcaagtact gttccaaatc acgttcatag ctttgtcgtg tattggcgga caatccccgt
     4441 tcaatattca agtagtggag ataatcgttg atttgctctt tcataataat ccccctcatg
     4501 cctctctatc atatcagata ccgcttcatt ctgatagtct ttttccataa tctgagcaaa
     4561 gaaaaagccg tctagacact cagacggact ggtttctttt ttcggctcta taatttatgg
     4621 gactgatgaa tcagaattga ttcttcagcc ccattttcat tttaggtatt aaa
//
LOCUS       DAZULY010000200         4611 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000200 DAZULY010000000
VERSION     DAZULY010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.202"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..400)
                     /locus_tag="ACG81A_002343"
     CDS             complement(86..400)
                     /locus_tag="ACG81A_002343"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5015667.1"
                     /translation="MLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQ
                     RYGATKIHQVLLKEGISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQ
                     KN"
     gene            complement(451..705)
                     /locus_tag="ACG81A_002344"
     CDS             complement(451..705)
                     /locus_tag="ACG81A_002344"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5015668.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(898..1173)
                     /gene="prgO"
                     /locus_tag="ACG81A_002345"
     CDS             complement(898..1173)
                     /gene="prgO"
                     /locus_tag="ACG81A_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFS5015669.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1145..2098)
                     /locus_tag="ACG81A_002346"
     CDS             complement(1145..2098)
                     /locus_tag="ACG81A_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5015670.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2710..4203
                     /locus_tag="ACG81A_002347"
     CDS             2710..4203
                     /locus_tag="ACG81A_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFS5015671.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4338..>4611
                     /locus_tag="ACG81A_002348"
     CDS             4338..>4611
                     /locus_tag="ACG81A_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFS5015672.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1543 t
ORIGIN      
        1 ttgcttgaat tcttgtgaaa aagttcttcg tgttctctta gacataaaaa ttcctcctgg
       61 tatgttttct tctagtctac acaccttaat ttttctgtct agttaattgt agcctatcca
      121 tgatcggttt attagacctt tgaggtctat attttttaac tacaatcgac cttaaattta
      181 attgtttcat tagcttttgg acatgtttaa gagatactga aatcccttct tttaataaaa
      241 cttgatggat ctttgtggca ccatagcgtt gtttattcgt aaaaaaaatt gttgaaatct
      301 tctttttcaa cttattattt ctgatttctg tagctgtcaa cggtttattc ttataaaaat
      361 aatagacact tctagggatt tctaataacc gacacaacaa agaaacagtg taatccttac
      421 accatttttc aataaattga acaagcgaat ttaggttttc cgagagaata tggctgcggc
      481 tttttttaag atttcaagtt cctcatttaa acgagcattt tcttttctca gttcagctac
      541 ttcttttcca gtcagtcctg tggactgatc aggtaaatat aaattcttcc atttgtaaat
      601 cgttgcttca gataagccat attctttcgc taaccctcga acagaacgtc cagtttgatt
      661 cagttcaaca atcatttgtt taaaattttc ttcatatcgg gtcataaaaa ttcatcctct
      721 cgtagataag tttaatttat tcttgtctct acgaaaagct gtctaagatt tgatactaac
      781 accaaatgtt gtataccccc aaacaataca catattttga aggattagga ttaataaagc
      841 taaaaatata gtaagtatac taaatatatt tactatatct agtatactta ctatatttta
      901 atcttttgat cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc
      961 gtccattgca agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg
     1021 aatggttttt agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt
     1081 tcgttcccct aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt
     1141 cttcttatct ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta
     1201 ggatatgatt ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac
     1261 catgttgttc atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag
     1321 cccaccatct tcgatctaca taatcattta attggatacc attctcacca tacatttcta
     1381 accgatccga gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg
     1441 ttttttgata gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc
     1501 caatgatttg aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa
     1561 taaaatcttc tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat
     1621 ctgcagcagt taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga
     1681 tataatcgaa attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac
     1741 gacgatcagg atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag
     1801 ccataatata taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga
     1861 ttgctgcaga catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag
     1921 tagtatttct ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag
     1981 aaacaatcgc taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa
     2041 gcattacaac ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag
     2101 ttccctccaa tttcctatac taaatatatt tagtatactt catatactta atatattcga
     2161 tatatctaat atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat
     2221 actaaatata tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg
     2281 tgcgttttgc gttgacaatt tagagtgaaa agaggtaaac ttaattctat agaaaccaca
     2341 gataattcac tatttacaaa gtagcaaaag cagtggtact ctttaaacag aaaaattaca
     2401 taaaaaaaag agacggaaat aattgatttt agcagaatca attatttctg aatcacatgg
     2461 ttaggacatg tgttcacgct cgtctcaaga atatgtaagt agctaccaac tacttatatt
     2521 aaaagttgat cggatacctt ttccgattgc ctttattcta ccaaaaaaag gcgagcaact
     2581 caacagaaga acgtttattt agtgtactta aattaaatat tcttaaacac tgaagagaga
     2641 acacatacta tggctgagat taaagctaaa ctgtgttctc tttttgtttg agaaaggacg
     2701 ttacaatcga tgttgagcga aatttattca gctattctta aaaacggctt acgtacatac
     2761 aaatatcgaa acagcaccat gaaacctgca ggtcatattg attccagtag gagtggtgct
     2821 atctttgcgt accgtagcaa gcaattaatg aatgttgggc gtggtatggt cattacttcg
     2881 gaagaagcta ttttagagaa cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc
     2941 tttggtactt atgcggatga ttatcgaaca gtggttaaag gtcattctga gaaaaactta
     3001 agtcaaatca acacatttgt agttgatatc gattcaaaag agaaccatca aggcgaaatt
     3061 attttagcct gtttagatca agtgggctac atgccaacgc taattttaga atcagatcat
     3121 ggttatcaag tttattttgt actaaagaca ccaggctatg tcacaaagaa aagcaatttc
     3181 aaggtgatcg atgtagcaaa aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa
     3241 ttgtccggag tggatctagg atgtaaccac ttcggaattg ctcgattccc acaagaaaat
     3301 aatattgttt ttaaagaaat gaactatcag tattcttttg ctgactggat tcaatggtcg
     3361 atgaagcttg aatcagatca aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt
     3421 cctgagaaaa aagaaattcg tcaagtagat gaaccgtggt tcgatatgtt acttcataaa
     3481 gcaaacatcg tcggcggaga aggacgtctt ggtcgaaata atgtgatttt cacgctttct
     3541 ttagcttatt actcttcagg ttatgcattg gatacttgcg aatacaatat gttcacattc
     3601 aacgaacggt tacaggaacc gctatctgaa gcagaattag taaagattgt acgtagtgct
     3661 tattctggta attatcaagc agcaagcaga gaattcatta ttgaattgtg ccagcaatgg
     3721 gtttcaccaa ccttaaaaaa taatgatctg ttcattcaac gacgtggctg gtggaaattt
     3781 aaaaagccac gagatcaaag aaaatatagt cataaaaatg aatggaaaga agatctattg
     3841 gcttatctaa gcgaaaaaag tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa
     3901 ttggcttcac aactaacgat gcccaagcga acacttgata agatcttaaa tgagttacga
     3961 gaggagaata aagtcttcta caaggtaaca cgaggaagaa atggcggaat gatcattgct
     4021 tcagtcaaaa cgttgttcgc gagggttatt caactaagta aagaaaagag actagattat
     4081 tcaacaagga tcacagaggt atttggactc aacgagagct tagtagaaga gacatttaga
     4141 caattatcaa cccccttgaa ccctcttaaa gaagtgaatc tttttgaagt agatacaggt
     4201 tgaaaaataa aaggtgcaaa ttgccttata caatatatct atgatacttc tgtaggtgtt
     4261 gctccctttg cctagctacg tgtaggtggt atacttataa ctgcgtatgc agtcttatgt
     4321 atcatggagg aagttgtatg tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt
     4381 ttatcattgg tcgtttggga ttatctgtgg aagtgttctg tgagctttat ggttttaagc
     4441 aggggacttt gtcttcttgg gttaccaggg aaaaaacagt tgcttctttg ccgattgagt
     4501 ttcttcatgg gttgtcctta gcttctgggg agactatgga tcaggtatat gattgtttgt
     4561 gtgtgttaga gcaggagtat attgagtata aaatagctaa cgagttaaga a
//
LOCUS       DAZULY010000201         4545 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000201 DAZULY010000000
VERSION     DAZULY010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.203"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            650..1561
                     /locus_tag="ACG81A_002349"
     CDS             650..1561
                     /locus_tag="ACG81A_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HFS5015673.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            2048..2314
                     /locus_tag="ACG81A_002350"
     CDS             2048..2314
                     /locus_tag="ACG81A_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015674.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2386..2727
                     /locus_tag="ACG81A_002351"
     CDS             2386..2727
                     /locus_tag="ACG81A_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015675.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2782..3201
                     /gene="ssb"
                     /locus_tag="ACG81A_002352"
     CDS             2782..3201
                     /gene="ssb"
                     /locus_tag="ACG81A_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS5015676.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3278..3574
                     /locus_tag="ACG81A_002353"
     CDS             3278..3574
                     /locus_tag="ACG81A_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015677.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3589..4020
                     /locus_tag="ACG81A_002354"
     CDS             3589..4020
                     /locus_tag="ACG81A_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFS5015678.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4325..4507
                     /locus_tag="ACG81A_002355"
     CDS             4325..4507
                     /locus_tag="ACG81A_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015679.1"
                     /translation="MQRTLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKR
                     FTEEIEQLAELNIRYF"
BASE COUNT         1658 a          717 c          978 g         1192 t
ORIGIN      
        1 ttatatgtaa taagtggggg gagggctgtc ggcgcttggt atgaaaggca ttctccgact
       61 tccaggtttt tgaccatttt ctctcaacgt ggaaaaaccg ccacttatga gagaaaatgg
      121 tcaaaaacct aagcgggaag tctccgaata acataccaag cgccgacagc caacagataa
      181 agaatacact actaaaaata gtagtagcat aaaatttaaa agggaaaaaa tcaaccccta
      241 ccccagtctt ggggtctcaa aactaactcc cccctaaccc cctctttcag cctcttttga
      301 aagaggggga gaatcaaccc cacggccaac gccccagtgt tcttggggct gtccagaaca
      361 gaaacttgcg agagagtggg ggtaaaacaa gtgtagagga aacttgaaga cttgttcgaa
      421 cagaaacttg cgagagagcc ggagtaaaac aagtgtagag gaaacttgaa gacttgttcg
      481 aacagaaact tgcgagagag ttggagtaaa acaagtgtag aggaaacttg aagacttgtt
      541 cgaacacaaa cttacaagag aggggagtaa agcagttgca gaggaatcag gaagatttgt
      601 tcgaacagaa acttgcaaga aagcattatt aaattgaagg tgatgaaata tgaagtattt
      661 attgcatggc tattttattg attattctac tcgcaatagt agtactttag cgattctatg
      721 tttgcttcat gaattgagag tggttactag aaaacagcta tacaaactat tgaattttga
      781 cagatcaatt agtaaacaag gagtaacgaa tgctttagtg agattatatg aaaaacagtt
      841 aatcgatcga aaaaaatatg gaacagaaat gtattattat ttgacgaaag agggacatca
      901 aagcataggt gggtattata cgttgccgaa agttcctgaa tataatttga atcaccattt
      961 acaaattaac aattatttga ttaaaggaat ggcattggca aaagaccacc cacatttaaa
     1021 acggattatt tcagaaagac gtaccgtcta tgaaacgaag gatttaaatc ctaagacaaa
     1081 aggaagaaaa ttttttgtgc cagattttat ttttcgtttt cgagacaaag aaaatcatga
     1141 gatcaattgg tcctttgaaa ttgagctaac gttgaaaaca cgtcgtcgct accgtgaagc
     1201 gatttttccg aaatatattc gtgagttgcg aacacgtcct gaatcacatt taatttatgt
     1261 gacacctagt ggcgcaattc aagaagaact tcatgcgttt aagcgtattt ttttcaagcg
     1321 cgaaactgaa aaagatgcga gtcaacaaga attgtttgag cggttgcata ttttttcagc
     1381 tttagagttt gaagagggat taaaagcatt attagagaat gatccagcag tcaattggcg
     1441 gacagtagaa cgagaaaaaa tttatccgaa tggcgctgtt caaagtcgtt tgaatttatt
     1501 tgaagaatca actgaaaatg aggaaaaacc aaaagtgaag tttacgcctg aagtattgta
     1561 aaagagggtg ttcgcttcgc tctttttttg tgccagtttg ctggcaccgt tcagacgcaa
     1621 gcgtctgaac cggctcgttt ttaggaagga ctaacttgtt tgatttatcc gttgtcaaag
     1681 aagtggacgt tcgagagtcc ctaatcgtat agcagtcatt cggcgagtat aacctaaaac
     1741 ataacctgcc aattgccaag ggtaaaatgc acgctgcgcg cccttggcaa tcgtcagaac
     1801 atgttttcga gccggttatg ccgaacgacc gctatacgaa aagggcctct aaacagaagt
     1861 ccactttgat ccttgacaac tgaaacccaa aatcaaacaa gcaggccttc gtctaaaaac
     1921 gagccgaatg aagaccttgg gggttgcacc cccaaacccc cgtgcccccg acacactacc
     1981 gaaaaggtgg gggtgggtgg cgaaaaaatc aaataaaaca ttctattcaa gaaaggaaga
     2041 aacaaccatg acaactaaaa acgagcaact gaaagaacag gcaagaaaag agttgcaaga
     2101 aagaggtttg attattgagg ggagctttga aggagatttt gaaacctata ttggttgtta
     2161 tgcaagaccg ataaacaaac ctacagcctt agacccaatg aacgaacaag aagtaaaaga
     2221 acaagaaaaa catgcagtga atggttttcc gcaagatttc actgaatggt atgagtggga
     2281 aattaacaac gggaagcttg aaaacttctt ataaaagtca gatgacgtgc aaagagaatg
     2341 attctctttg tacggtaaaa atataaagaa agaaggaaaa agaaaatggc aaaaaacaca
     2401 ccttatcaag taggagacat tttttatagt agttggggct acgaacaaac gaacgtgact
     2461 ttttggcaaa ttgtgaagtt aacagaaaaa acagcatggt ttcagcctat tcaaaagaaa
     2521 acggtcgagc attattcgtc catgagtgga aaagttgttc caatgaaaga caagtttata
     2581 gagtatcctt tagcaaaaac taaagacagt attgtaaaaa aacgaattga tccagaaacc
     2641 tatcctgata tgttatgtgg ttatcgtagt tgggacacgt ttcatagata taaaaatcaa
     2701 gctgtttatg aaagtagtta ttattaaggg gatagtttcc cctttcaaaa ataataaata
     2761 gaagaatgga gagaaaaaat catgatgaat tcagtgaatt tagtaggacg gttaacaaaa
     2821 gaagtggatt taaaatacac accaaaaggc aatgcgacag gcacatttat tttagctgtt
     2881 aatcgaaatt atacgaatgc aaacggcgag cgtgaagcag attatatccg ctgtgtcatt
     2941 tggcggaaag ctgcggaaac tttagcaaaa tatactggaa aaggaagttt gattggcatt
     3001 aatggacgtc ttcaaacgag gagctatcag aatcagcaag gacagaccat gtatgttacg
     3061 gaagttctag tgacagattt ctatttgtta gagagtaaag aaatcaatga gcaacggtca
     3121 aaatcaatgg cgaataatca aaataattct tcaattccag gaaatgagat tcaaatttca
     3181 gatagttatt tgccctttta agtgaaaaat aattttgttc tatacctgat ttcttcaggt
     3241 atagaacaaa aaataaatag agatgaggcg attttttatg aaaatcattg acagttacga
     3301 tcaacgaacc atgcaatatt tgggctcgtt tgattataca gatgaaaata ttattaatta
     3361 tgttgcaagt ttacctcctt atcaaagcgt tcgcttggta gattttgcaa gtgatgaatt
     3421 ggtattaaca acgatgggca actttttaga ttatgtcgga gacaaaaaat ggttggaaaa
     3481 aattttgtct aaattgattg cgaaacaaac aggagaagaa cccataaatg cgattcaatt
     3541 gttcaatcga tatgaacaag aaaacaatga ttaaagagga gagaaaaaat gaaaagatta
     3601 aaacgaagta tttttattgt gctagcattg attgattttg ttgggacagg ttgtgaagca
     3661 attgacgttc aaaaagctcc cttatcggaa ataaaacaag caataacaaa acaagatgaa
     3721 gaacaaccgc taaatacaca agtagaaata gtgaatcaaa ataaaccgag tttttctgaa
     3781 gaagattttt cgttagaaaa tggaaccagg caattttttt cagatttgga tcgtttaaat
     3841 cgggtaggtg tagcaagtgc acttttgcat aaaagcagga tgccaacaga agaacgagac
     3901 cgattataca ttaagccaac tggctggaaa caaaagaaaa tgaagaatgg cgattatctt
     3961 tataatcgtt gtcatttgat tggctatcaa cgtgcgacaa gtttccccat aagtatgtaa
     4021 tacgaaacgg gaggattaat cttaagatta atcacaacta acctatttga cagttggaat
     4081 gaaagctatc atgtctgctg aatcaactgg tctgatactc ctacatgcaa taaagtctac
     4141 taggaagtta ttgtatgaag ctaggtaaag gcggcagaac aatttctaag tgtggaatca
     4201 tatgaagttg gatatgccgg gacgctataa attactcagg gtgagactgt ttttaattaa
     4261 actgacgaac ttgcgaatgt acgggtctat aaccgactta tattcgaaag atataagatt
     4321 gcgaatgcaa agaacattat cgaaaagtaa gaatgttggt catgaaattc gagatggaag
     4381 actagcggtg tatctgtcaa gagttggtaa aacatgtttg attgattcag atgagagtgg
     4441 gaatgcgaca aaacgtttta cagaagaaat agagcaacta gcagaactga atatacgata
     4501 tttttaaata ctttagcgga gaataaaaaa atcctctaca gaaaa
//
LOCUS       DAZULY010000202         4535 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000202 DAZULY010000000
VERSION     DAZULY010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.204"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(83..955)
                     /gene="scrK"
                     /locus_tag="ACG81A_002356"
     CDS             complement(83..955)
                     /gene="scrK"
                     /locus_tag="ACG81A_002356"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS5015680.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            complement(991..1458)
                     /locus_tag="ACG81A_002357"
     CDS             complement(991..1458)
                     /locus_tag="ACG81A_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS5015681.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            complement(1468..2937)
                     /locus_tag="ACG81A_002358"
     CDS             complement(1468..2937)
                     /locus_tag="ACG81A_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5015682.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            complement(3008..4426)
                     /locus_tag="ACG81A_002359"
     CDS             complement(3008..4426)
                     /locus_tag="ACG81A_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFS5015683.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
BASE COUNT         1440 a          946 c          653 g         1496 t
ORIGIN      
        1 acttttaggc gtcccttcaa ttactaattg aaaaaaagat cgcatcaacc aaacaaatag
       61 tcgagacgat cttttttcca aattaagatt tttctttaac gtctcttgct aaagctaaac
      121 aacctacaat accagaatta tctaacaatt ttggtgtcat aatataattg tcaacatcgg
      181 gaatagcgac atatccgttg atcaactcaa taaacttatc ttgtactttc ttttttaagt
      241 gtttttgttt cattactccg ccaccaaata taatgacatc aggtgataac atcaatgttg
      301 tattatatgc acactgagca atgtaataag cttctatttc ccaagtaacg tcattttcag
      361 tcacttcttg tccctttttc cctgatcttc tttcgattgc cggaccagca gctaagccct
      421 ctaaacagtc tccgtggaat ggacatgtcc cagaaaagtt gtctttattg tgtcgtttta
      481 caagcatatg tcccatttct ggatgactaa aaccttcaat gaagtttccg ttttggatag
      541 cgccacctcc tatgcccgtt cctacggtat agtaaacaac acttgaaagg cctctgccat
      601 ttccagaaac atattcacca tagcaagagg catttacatc agtcgtccaa gcagttggaa
      661 tattaagttg cttttttaaa cttccaataa aatcaaaatc ctgccaagca agttttggcg
      721 ttgatgtaat gaagccatac gttttagatt cacaatgaat atcaatagga ccgaacgatc
      781 ccactccgat accttctaaa tcatctttat gatgattaaa aaaatctaaa actaaattca
      841 ttgtttcttt gggtgtagtc gtgggaaagc tgactttttc aaatatgttc aaatctttat
      901 ctccaatggc acaaacaaat tttgttccac cagcttcaat agccccaaaa accatacatt
      961 ttcctcgatt ctattattaa ttacttatca ttagccaaga acaaacaatt cttgtccttg
     1021 ttttacttct tcaagtttcg gaatgaaaat ttcgtcttgg tagtctggaa aaacaacaat
     1081 gacatcttta gtcttgtgat tcttttcaaa aactacaaaa ttaatggtca taagaatatc
     1141 cccagcctta actttttgtc cttcttgaac tttaatatca aatccctcac catttagctc
     1201 aactgtatct attcctacat gaacaagtat ctcaattcct gattttgttt ttattcctaa
     1261 ggcatgtttt gtaggaaaaa tacttgaaac aaaaccatcg attggagata caatcgtttc
     1321 gttttttgca tcaattgcaa atccttgtcc catcattcct tttgaaaaga ctggatcact
     1381 taatttctcc agtttttcaa aaacaccggt tgcaggtgct acaacgctta tttcattttt
     1441 tctaaaaaaa tttaacatgc tctcatctca ctttacttga actggcaatt tttttgagtc
     1501 agttttttct gcaaatttac ggtgtttagc ttcagggata aacactttat atttatcata
     1561 agcgagaata aaaatgattg gtaaaatgaa tgctatgaga ttaccaaata tataaggaag
     1621 aactggatta gcaatagtca gacctggcaa tacagtcaat ccttgtccaa tggcttttac
     1681 atcaaaaatt ttcataaatg ctccaccaaa accagcacca ataaacgagc aaacaaaagg
     1741 aacgattgaa tatttcaaat taacagcaaa gatagccggt tcagtaatac ctaccagtgt
     1801 tggaataaag cttgaaatcg caatatctct atctttagat tttttcttaa agagtaaata
     1861 cataccaata caaccgccac cttgggcaat aattgaaact gaccaaatag gttggatata
     1921 attgaatcca gttgttgcga ctaagtttgc ctcaatacct tgaatagcat gttgtgttcc
     1981 tgttacaact aatggttgta acgcgcctgc aaaaacaaaa gcaccaaagg cacccatctt
     2041 catatatagg aaatcaatga ttccacctaa aacattaccg ataaaattcc ccaatggacc
     2101 aaacactaaa aatagtgcaa agttggcaag aacaattgtc acagttggga ctaaaatgta
     2161 agcaagtact tgaggagaat atttatttgt aattttctca acttttgcta aaaaccaaga
     2221 agtcaaaatt gctggaaaaa cacccccttg aaacgcaact gctggaatgc ttaatccaaa
     2281 taaatgccaa taatcaggct ttatagttcc catgacgaaa tcattccgat tggctagtcc
     2341 tggatgaatc ataacaaatc ctacaagtaa tcctaaaata gggtttccgc caaaacgttt
     2401 tgttgcagaa tacataacta aaactggtaa ataatcaaat gtggttgcaa taatacccag
     2461 aaatttggat aaatcattta ggaatacatt gtgatcagcg aatgagccat ccattccaaa
     2521 cattccagga gtggtaatca acgaacgaat tcctaaaacc attgctgccc caataaaagc
     2581 tggaatgatc ggaataaaga tatccgagaa aactttaaaa gctcgttgaa gtctatttcc
     2641 tgaattattg actccttcgt ctttcatttc agaaacggat acttccttag ctcctgtcaa
     2701 ttcaacgaac tctttgtaaa ccttttcaat tatacctgtt ccgaaaatta cttgtaactg
     2761 tccactattg aaaagaacac cttttgcacc ttctatttcc tcaagcattt ccatattcgc
     2821 tttgcttttg tctttaagaa ttaaacgtaa acgagtaatg cagtgtgtag cgcttgagat
     2881 attctctttt cctccaacgt tttccaatac atcttcggca atttttttgt agtccattca
     2941 tttatcctcc cttttaaatg tgacaggtca tgtttgattt tcagacctgc cacatgattg
     3001 acgtttatta tttctttatt tcatttgaaa acttggtatt tattgcagtc attgatccat
     3061 ttgttgctgt aatagtaatt gcttgaccaa aacaaccatg tacacgagcg ttaagagcga
     3121 ttccatcaat ataaagtgtc attatattgt catcaacaat cacttttagt ttataagatt
     3181 ggttaggctt taaagatata ggtctaataa gtcctttatc catatattga aaccaagggt
     3241 aacaagggtt tttatcaaat gtaagacgcc cctcatctaa attgaagcga tattcgtaag
     3301 attcatctgt ctcaaggttt ttatataatc tcagtgaaaa gtcactaaga ttctcatcaa
     3361 atgaaattgt tgtttctaaa ttaaacaaag ttccactatt tttgacaagc gttacttctt
     3421 ctcttgtgtc tgctgtttct atctctacat cggcaatatc atgtaaattt atgaatgcat
     3481 tccacacttc gtctatgggt tttaccccaa gtgaaaaatc ctctttttga tagacttcat
     3541 gaggaaccaa agtacctccc cataaataat tgtttttatc attttcttga tcttttgttg
     3601 gtacccatcc aaataggact cgacgttttc catcataagc tgttctacct gcataataag
     3661 cccgtccatc aaaagcttca tctttaggct taatccaagg accttccaaa ctttgactca
     3721 ttcgatagag tattttattt ccatctgagt attctgaata gacaagatac caccagtcac
     3781 caatcttgaa aagttctggc atttcaaaca tggtatacaa tcccggagcc caaaaatcac
     3841 ctttgaattc ccaattagtt aaatcttttg atgtaaattc cactaaacgt ccggtttgta
     3901 gatgtttatc ctgacccttt cgcgccccta aaattagcaa atattcttct ttttcatcat
     3961 cccaaacgac aaatggatct ctccagtttc gaatatcata cccttcttgt ggttctaact
     4021 ccaatgctgt ttctgatttt tcccaatgaa caaaatcatc actctcagca tgaagcaaca
     4081 cttgagatgt ctttccctgc gctaagaatt cacgattata ccctgtataa aatgcgtgaa
     4141 cctttccatt tgcctcaaat acagaaccag cgtagataaa ctggtctctc tcattgtcat
     4201 cccctcttaa aagagattcc ccaaagtctt caaaatgaac aaaatctttc gtagttgcta
     4261 aagcccagcc aaacggttct ccaaaaggaa ctggatttct agtatcacgt tgatgataaa
     4321 taaagaattt tccttctttc ccgtaaggca taacatctcc gacccaaaca tccttaggtt
     4381 ggtagtaaag ttgatgatta ttgcttttaa ttaataattt ctccattttc ttcctccagt
     4441 gaaaaggttt ttatcttcac aactaatata tgtcaacccg tttcatatgt caacccgttt
     4501 cataaaaata tttttaaatc gacgaaggaa ttttt
//
LOCUS       DAZULY010000203         4520 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000203 DAZULY010000000
VERSION     DAZULY010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.205"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1259)
                     /gene="dltD"
                     /locus_tag="ACG81A_002360"
     CDS             complement(<1..1259)
                     /gene="dltD"
                     /locus_tag="ACG81A_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFS5015684.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKL"
     gene            complement(1262..1495)
                     /gene="dltC"
                     /locus_tag="ACG81A_002361"
     CDS             complement(1262..1495)
                     /gene="dltC"
                     /locus_tag="ACG81A_002361"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFS5015685.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            complement(1540..2745)
                     /gene="dltB"
                     /locus_tag="ACG81A_002362"
     CDS             complement(1540..2745)
                     /gene="dltB"
                     /locus_tag="ACG81A_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFS5015686.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            complement(2742..4256)
                     /gene="dltA"
                     /locus_tag="ACG81A_002363"
     CDS             complement(2742..4256)
                     /gene="dltA"
                     /locus_tag="ACG81A_002363"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFS5015687.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            complement(4267..4416)
                     /locus_tag="ACG81A_002364"
     CDS             complement(4267..4416)
                     /locus_tag="ACG81A_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFS5015688.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
BASE COUNT         1410 a          916 c          758 g         1436 t
ORIGIN      
        1 tgtagtttat ctggtatttg atgttgccaa gatttgctaa aaaaagcatc gtcaagatga
       61 tacttactag cagtaatatg attttcttct aaaaacggtc tgatctgttg atctgcagcc
      121 aaccagcctt tccagcctag atggatcgtg tcttccataa aatagttggt acccgcttga
      181 ttgacaaaat cagctattcg attgaatcct tggctattta attgaaattt gatctttttg
      241 gcaaatcctt gtaacatttc ttgagatagt ccggtataat cgctccattt ctcgttaacc
      301 ggtgggatga tgaacaatac ttcgttatgg ttttttgcta attgatccaa gaccagctga
      361 aaatcagaaa attctggcga aaaacgataa tcccaatttt tttgtgactg tttgagctct
      421 ggctcgtggg ctcggatacg atgcgtataa aaatcgtttt tcaacccgaa tggattattc
      481 gtcgtttctc gttctccaat ttgatttgct aaaatggata attctgtttc ttggtattgt
      541 tttggtaatc gttttgactc atgatcgatt ttttgctgtc gatctttcaa cccaatattg
      601 ctgaatactt catcctctct cttcagcatg ttccattggc tgcgcagctg attcaaagat
      661 tcaggtaaag gtagcttccc ttttttgatc gtttgcagaa tcgccgtcaa cgtatcattc
      721 tcttttactt ttgagaaagt taataatctt ctagctaaat agcgatcagc aggcgttact
      781 ttcttcattg aaaacaacca gtcgtatgtt tgaagttcag aataatacgt attaaagtaa
      841 tcagttttta caccattttt aacgaaccac tgaggagaaa taataaagac gactttcttg
      901 tttttcatag catctcctgc agaacgcatc atcatatatt ggcttaacga ttgtgtcccg
      961 ggtgctccca gcaaaaatgg acgatagttt cgctggtatt tttctgccag aacagacgga
     1021 tgaaatggac tgattctgct taattcactt gagccaaaaa aaggaatata ttgtttttca
     1081 gataatgcat aattttttat cgcatctcct ttcaacacgt tcactgacat ggaagaagag
     1141 gcacgtctaa tcgtttctgg attagagata tctacttgat aggaggaggt aaaaaacaca
     1201 aacagtaagc caacagctaa aacaaccggg ccgaaaatag caaaaagttt ctttttcata
     1261 ctcaaccctt caatgcagtt gcttgttgaa tgattttatt cggtgtgctc caatcctctc
     1321 gctcgaattc agatacaggg acttggatat ccagctctcc gtctagctcg actaataatt
     1381 gaacagatgc taaagaatca agcaaccctt catcaaatag atcaatatca cgattttttc
     1441 ttacttcatc tgttcctgtt atttcttcta agattgataa aatttgttct tccattttcc
     1501 ttttctcctt tgttatataa attaatatgt cactattcgt tagctaaaaa aaagtttatc
     1561 taaaaatcct gaaaaaatca ggaagctaaa acatactgct tgaaatgtca aaaatacggc
     1621 taagccatgc gtaaatttgt tagaaggcaa ctgctcttta tgcttcttct taaatcggat
     1681 ccatgcatct gtcagacaga tcagacaagc atgatacagt ccatatacga tgtagtacca
     1741 cgtcaatcca tgccatatac ccattagaag aaacaaagca aaataaccga tatttgacat
     1801 tacgatcttg cttttaaaaa attttttctt catcatgaag aacatcaatc gcatatatac
     1861 ataatcacgg aaccaaaagg acagcgtcat atgccaacga ttccagaatt ctttgatatt
     1921 ccatgataaa aaaggtttat tgaaattgat tggtgtctga tatcccatca tgtagcttgt
     1981 accgactgca aataagctat agccagcaaa atcaaaaaac aaatacatac tgtacacgta
     2041 catataacct actaatgccc atgaaatacc accatgctgt aaggcaatgc gtcccactac
     2101 aggaagcagt acttgaccaa aataataacc gataatgaat ttatataaaa aaccgacaaa
     2161 aatattttgg acgccttttc ccaaaagatc aacatattta tcacgaacag gcggatggag
     2221 ctgatctttt tcaaatctcc tatagcgatc gattggtcca gaagagattg ttggaaagaa
     2281 cagcagaaac tggatataac gaaatggctt atatgttttg atcatgccat ctcttatttc
     2341 catgatcatt tgtacagatt taaaggttaa ataagagatt cctaaaaaac caagtaacga
     2401 tgcttttcca tgttcaaaaa aaggagtcac tttcgttaaa aacagtggaa ttatcgctaa
     2461 aataacggcg gcaaaaaacc atcctgtctg atttttcctt tgccgataaa caaaatagcc
     2521 ccatacaagt atcgtctgcc acaaaacata cccaatcagc gccgctccct gttgccaata
     2581 attacctcca aagctgacaa ataaaaagaa aagtgtcact gctgattgat aaactggtaa
     2641 tcttttttcc catatcaata aagatagaac gatcggtaca agcgcaatgg ctaaaagaaa
     2701 aaaataaaac ggctctgcat aagggatcat atgaggtaac atcattcagg attcacctca
     2761 gctgttaatt tttttcgatc gatttttcca ttaactgtct gaggcagcag atcgacataa
     2821 tggaacttct gaggaatcat ataatccatt acaacttcta gtaattgttc tttgatagat
     2881 ttagtcaact gaaaatccgg ctgtttcgtt ttgttttcta atacgacaaa agcaaccagc
     2941 tgctgtactt ttgttccttg gtattttggt acgacgacag cttgtttgat tcggttgtct
     3001 tggagcagat aatgttcgat atccccgagt tcaatccggt atccatgtaa tttgacctgc
     3061 tgatccatac gtccttcata aaataaaagc ccatattcat ctagtctacc tgcatcccct
     3121 gtatggtaac ttgctaagtc attttgacaa aagaacgcag cagctgtctt ttcgggattg
     3181 ttcaggtaac cttttgatac gcttggtcct gcaataatga tttcacccgt ttctcctttt
     3241 gaaacaggtt taccttcttg gacaatagaa actttcgtgt ctttttttac gtagccaatc
     3301 ggtagtcggt cataactatc tagtacatgc tggtcaatct gaatacttga aatagctaca
     3361 gttgcttctg ttggaccgta agtattatat atccgtgcat ttgggaatcg atcaagtaga
     3421 ctttctgcag tttttttcgt caattcttct ccgcaaaata aaaacatgtc caattccggc
     3481 acattttctc cattgaatgt aggttccatc aagcaaatat ccataaatga tggagtagaa
     3541 acccacacat tcaacttcaa ggctggcaat gtcgcgaata attgacgaaa gtcttgaaca
     3601 actgctttat ttaacggctt cagcattccg ccagaaacta atgcaggata aatagaaaat
     3661 accgataaat caaaagaaaa tggcgcttgg gctaagaaat gcataccagg cttgatccca
     3721 aagtcttcca tcgtccattg aacaaagctg actagattat catgactgat ttgtacgcct
     3781 tttggttctc cagtagtacc agaagtaaaa ataatataaa aattttctgt accttttacg
     3841 ctatttctgg gataaaatcc tactggtatc ttcttgattt cttgaaattc tttttttgtt
     3901 atgacaggaa ccgaaggaat atcttctatc cagtccccga tactgatgac catagatggt
     3961 ttagccaccc gaaagatcga taggatacgc tccttgggtg tatgctcttc aatcggcaaa
     4021 taggcatggc cagatttgac aacccctaga aatgagacaa tcatttcaaa ctccaaattg
     4081 ccaaaaacga caattggtcc atcgccttct accttttttt ctaaataata agcaagtgcg
     4141 tctgatgctt gtttcaattc accaaaagta tgcgtctcat ccgcctgata agcaactcga
     4201 tcaggttcat ttattcccca atcatcgatt gcttcaataa tcgttttgat ttccatactc
     4261 ttcctcctaa aattcgttat agataaaagt tcctccttga atatttttat agtgatacag
     4321 ataaatcagg aacagcagta ctgcactata aaaaattgtt cgaatgatga attttcccca
     4381 ataattcaat tttggattag aacgccattt gttcattgga ttccctcctc atataacctc
     4441 acacaaagtg taactaattt tgacaaaaag aaacatgtgt ctagcttaca gaaaccgttt
     4501 ttttactaac agttttgtca
//
LOCUS       DAZULY010000204         4440 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000204 DAZULY010000000
VERSION     DAZULY010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.206"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(3..977)
                     /gene="lacD"
                     /locus_tag="ACG81A_002365"
     CDS             complement(3..977)
                     /gene="lacD"
                     /locus_tag="ACG81A_002365"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS5015689.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(974..1432)
                     /locus_tag="ACG81A_002366"
     CDS             complement(974..1432)
                     /locus_tag="ACG81A_002366"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFS5015690.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(1429..2892)
                     /locus_tag="ACG81A_002367"
     CDS             complement(1429..2892)
                     /locus_tag="ACG81A_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFS5015691.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(2874..3806)
                     /gene="pfkB"
                     /locus_tag="ACG81A_002368"
     CDS             complement(2874..3806)
                     /gene="pfkB"
                     /locus_tag="ACG81A_002368"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS5015692.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            3948..>4440
                     /locus_tag="ACG81A_002369"
     CDS             3948..>4440
                     /locus_tag="ACG81A_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5015693.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDS"
BASE COUNT         1322 a         1010 c          716 g         1392 t
ORIGIN      
        1 tcttacttgt taacataaat agaagttgct gtttcttcta atagtgtact taattcttta
       61 atattttgtt ttccttcttc taacatccaa ctactcgctt tttcttcgcc ttcggtaaca
      121 aaaggtgcta ctcccttcgc ccatgttgct cgcccacata agacaccatt gaatgtcgaa
      181 cctgattgtt ttgcaaaacg caatgtatct tgaaatagtg cagcgctgac tcccgcactt
      241 aaaaaaataa acggttgttc agtcactttc gattgttcat agaaatacgc ggatgcctct
      301 gcttgactat aacaagtatc ttgcccatat ccttccacaa atctcatatt cactggtact
      361 tctactttca atacatccac ttgataacgt tcttttgaaa attctcgcat ggcttcaatc
      421 actttatgcg gttttacttt tgcatagtca agagaagacg gatcgccaat agttgcatcg
      481 taagaaagga gctctaaaaa gaaaggtatg ccttctgctt cgcattctga accaatccgt
      541 tctataacta tttgtttttg cacattaatc tctactggtt catcaacgtc atagtacaat
      601 aaaaatttac aagcatccgc acccacttct tttaaccttt tcactgacca actattcagt
      661 aaatctggta gtcggcctgg cattttcgca tcatagccgg ttttttcata agctagcaac
      721 aaaccacatt ctttctctcg aagcgctgct gcaggcagac catattctgg atcgagtaaa
      781 atagcggaag catacggagt caggtactga gaaaccatct gtttaaattg gatgatatct
      841 tctgatgtag cttctttata ttggttgatc attttcttca aagcaccacg ttggtcgata
      901 gctagcgcgc taataattcc agcttcatcc gtcaatcgct tcaaacgttc ctgtttttct
      961 atactcattt tcttcatata tcttcaccta tttctttagt taatattgta gctatttcta
     1021 tttttgattt tgcagctctc aactgttctg taacctcttt tcgcatcaat gatcgtgcaa
     1081 cgagagacaa taatttcaaa tgagtcgttc ctgcttctga atctgggata aataacgcaa
     1141 aaataaaatc aactgactgt ctatctaaac tgttccattc aattcctttt ttcagtgtga
     1201 cgataatcaa actaggttca ttgattgttt gagacttcgc atgagggata gcaaaacctg
     1261 acattattcc tgtagtacct tctttttctc tttccagaag tttttgatat acttcttttt
     1321 cgtcatgagc gattcctaaa cagaatgttt gctttgctaa aaacttaaat gcttcttctt
     1381 gattatctaa tggctgttgt aaaaaaatgt tctcttcatg aatgattgtc atgataaacc
     1441 tcctgctctt ccttcttcga taactgtacg atttgttgct ttattatccc tcttcttgtc
     1501 tttatcagtt actcttttct tttggatcaa acctagtatc actccaccaa cgatcgaacc
     1561 aataagaatc gctgtaaccc attggatttt tcccgtcact acaggtaaca ctaaaaaacc
     1621 gccatgtggg gctggtactt ggacaccaaa cagataggtc aagacagcag caattgatga
     1681 gcctaacatc atcgttggta atactcggat cgggtctttc gcagcaaacg gaatagctcc
     1741 ttccgtaata tgagttgatc ctaaaataaa attaaccaaa cctgcattac gatcattagt
     1801 atcaaaatat ttaccaaaca ataaagtagc aaaaccagta atcaacggtg gtgcaatgca
     1861 agctgctgac gcaccagcca taaaagtcgt gtttccttgt gtcaaaagag ctgttcctgt
     1921 tacatatgcc gccttattaa ccggtcctcc catatcaaac gcacacatac agccaacaat
     1981 cacgccaaga acaagtggat tcgaatattc aaatccagct aaaaagctca tcattccttc
     2041 atttatagcc ttcattggac ctgccagcaa agacatcacg atgccaataa atgctacacc
     2101 aataactgga tataagacga ttgccttcaa gccatctagt tgtttaggta aaattatcag
     2161 tagcttttgc aaaaataaga taaaataacc tgctaagaac ccagctaaaa tacctcctaa
     2221 aaatccagta ccgccattca tggctacgat tccaccaaca aatccaacga ctaaccctgg
     2281 acgtttcgcg atcccttctg caatataagc tgctaaaact ggaaccatca ttcccattgc
     2341 tgcatcgtca actgctttta ccattgccgc tgtagcattg tattgtgcat gatctggatc
     2401 agaagaatag attccccaaa cggcaaatga aatagcaatc aatactccgc cagctaccac
     2461 gaaaggcaac atatgagaga caccgttcat caaattttta tagatgctac gaccaaaatt
     2521 agcaccactt atcgcttctg gttctaaagt atcagcagct tcattatctg ctaagacagt
     2581 accttttcct gccaatgctt cgtcaatcag ccgttgtgca tccttgattc ctgtactgac
     2641 agaaacatca atgactcgtt ttcccacaaa tctttctgaa tggacatctt tgtctgcggc
     2701 aataatgaca gcatctgcct gtttgatttc ttcagccgtt aattcattct cgatcccaat
     2761 ttgtccatgg gtctccactt tgatcgtgat gccttttttc ctagctgctt gctctaatgc
     2821 ctcttgcgcc atgtaggtgt gcgcaattcc tgtcggacaa ccggttgctg caattagttg
     2881 atattttccc atctttcgaa cctctcttcc ttgattttga tttgtttact taattcctcc
     2941 acatccaaaa aatcagtcag tcctttacga aaagcagtag agctgcctgc tgctatgctt
     3001 ttacgtaacg tttcatcaag cgatcgtcga ttcatgtatc ctgctaaaaa agtgcctaac
     3061 atggcatcac ctgcacatgc tgtattgaca acttttcctt ttggcgagtt tcctcgaatc
     3121 accctgtctt tcgtaaaaag aatcgctcct ttctcaccga gggataacaa aacattttct
     3181 gcgcctgact ctactagtcg ttgtccataa acaaagtaat cttcatctgt aaccattttt
     3241 cgtccgaacc aagaagaaag ttcttcctca tttggtttca gcaaaaacgg tcgatagggt
     3301 aaacaatcca tgacttcttg atcactacta tcaataatca aacaaatccg actgatttct
     3361 attaaaacct ttggagacag ccctcttggt aaactcccag aaacacaaag ataatcccct
     3421 gcttgtaatg ctcttatttg atttaaaaaa ttatgaacag ctgtctgcgg tatttcaggt
     3481 ccttgattca ctaatttgta ttcttctccc gtttgattga cttgtgtaaa tacattgata
     3541 cgcgtcatac caggcacttc aataaactct gtttggattt gtttctgctg taaatagtct
     3601 tctatatact ttcctgtgaa cccagcagaa aaacctaatg ccgtacttgg gaaatctagc
     3661 atctttaata ctagtgacac attcacccct tttccgtttg cctgaatgtc atcctctttt
     3721 gtgcggttta ccataaaagg atgcatctca tctgtttcaa taaatagatc aattgccaaa
     3781 ttcatcgtgc atgtgtagat agacataacc aacgacctcc ttgattctat tatgaaatac
     3841 gcgaaataga aaatgttttc ttaatcgtcc caacacaaag aaaaccttta catttataaa
     3901 ctcgtcgtgc tttttttaac taacttttgg tgaaagaagg aaagaatatg aatcacaaat
     3961 taagtgaaac agagcagtac ctctggaact ttatcgaaca cctcatccta gagatcccta
     4021 actattccat cgtgaaacta agtgaacaag caaatgtctc aacagccacc attgtgcgga
     4081 caatgaaaaa gaaaggctat gaagggttta cttcgtttaa gcatcatcta aaagaaggag
     4141 aaaacaagaa catcaatttt tcatttttag ataaagtaga caaaggaatc cgccgagcca
     4201 ttctaaaaaa tgaacaagaa gtggtacgta caatcaatat gttggaaatc gggaacattg
     4261 aagatgcaat acaaagaatc aaatttgctg accgggtttt tgtctttgcc cgaggatttt
     4321 cagaaatgat tggccaagaa atgctggtaa aacttcaatt aacgggaaaa aattgtcaga
     4381 tgcatactga tccagaaatt atcaaaagca tcagtcggaa actaactaag aaagacagcg
//
LOCUS       DAZULY010000205         4411 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000205 DAZULY010000000
VERSION     DAZULY010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.207"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            262..990
                     /locus_tag="ACG81A_002370"
     CDS             262..990
                     /locus_tag="ACG81A_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFS5015694.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1135..3162
                     /locus_tag="ACG81A_002371"
     CDS             1135..3162
                     /locus_tag="ACG81A_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFS5015695.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3270..4367
                     /locus_tag="ACG81A_002372"
     CDS             3270..4367
                     /locus_tag="ACG81A_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFS5015696.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1546 a          713 c          917 g         1235 t
ORIGIN      
        1 gtcacatttt gatagatagg agggggactt acgaattatt ttaaaggcta tgacagctgt
       61 atttctagat tgtgtttgca tcaagttgca ataaaaacta gaaatcaaat cgggctgata
      121 atcatttata actaatcgat acataggacc aaatataggt ctatgttctt ttatttcata
      181 agtatcgtaa aacagaattt tgctatggat taatccaatc gactgatatg tttagtaaat
      241 aaagaaagag gaatgtcaat gatgaaaaaa gaaatcgtat taacagcagc aatgatgtta
      301 ttcagcatgg tagcaagtac gacttttgtt tctgcaactg aagtataccc aaaggaatat
      361 aacacagaag gaacgattac atttgaagca ggagatgagg gtgtaactcc tcctgttgat
      421 ccagagaatc ctgatcccaa taaacctgtc gatccatcag atcctccaag ccctggaaca
      481 ggaggggcat tatcgattga ttatggatca aaattcaaat ttgggactca aaaaatttct
      541 actgcggata aaacgtatta tgcagctgct gatgtgatga acgatgggtc tagaaaaccg
      601 acatatgtcc aagtgacaga taggagaagt accttatctg ggtggaaatt aagtgtctct
      661 caaccagaac agtttaaaac tgccagtggg gatgaactag ttggagcaca gctgaaattt
      721 acgaaagggc aggctgtttc tttggttgat ccaacatata cgccgcaaac ggtgaattct
      781 gaattaacgt tgacaccggg aggaaacaat acgttagcaa ttaatgctaa atcaggaaca
      841 ggtgtgggga cttgggtcta tcgatttggt gcaaatgaaa atgaaaatca agatgcggta
      901 cagctttcgg tacccggtaa atctgtcaag ttagcgcaac aatattccac taaattagtt
      961 tggacattag aagatacacc taacaactag tttactgaaa agaactgtta aaaataaata
     1021 cgtagtcata ttgtcttgcg aggtaatcga gcaaaaattg tcaaaggcta ggctaaattt
     1081 gtttggatac gaatagttca tctaggaatt taacgatcag gaggaaaaga tatattgaaa
     1141 aagaggagga tcgtgctcat tttaagcagt ctcttagtga ctaatatctt tccgatcata
     1201 gggtactctt tggaattgaa tcagttggag catggaaata gaagagacga agaaagcaaa
     1261 ctcacttcga cattcgacag tttaatagaa tccaaccaga cacaagaaag cagtgaggat
     1321 ttaagtattt cgagtagtgg agagaccact gacaacgtag aagctgaggg ttcggacaaa
     1381 caaggactag aagatgcacc aatttctgcg gttgagctgc ctgacgatcc caatattatt
     1441 tctatcgata aaatttttca aacacctatc ggtactagca cgtccatatt ggaaaatggg
     1501 aagttactgc agttgaaccc agcagaaagg tctcaaagag gagctatttg gtcaaataag
     1561 aagattagtt tactctcgga ttttacattt aaatcttatt tatacttagg aaatcaacga
     1621 gatagagcgg gagatggcat gacctttaca ttgactggcg atcctagaat ggctagtaat
     1681 cccagtcaag ttattggatc gccaggtatg ggaattggct catattctac tcgttctggc
     1741 caaccttaca ttgcgaatgc gctctcgatt gagtttgata cgtataaaaa tagtggttct
     1801 tctgatcgaa tggatcgaga gataactgct gataatagag ggcatggaca tgtcgctttt
     1861 gtcacgccta aaccaaataa taataattat tctggagagc atactggcgt gacggtagct
     1921 cctacatttc tttctaatgg cacatggcgc ttgttaataa ttaaatggga cgcggagcaa
     1981 agaagtctta cttatgattt ggaaggtgtc ggtcactctt ctcggattat acctgatctc
     2041 attaggcagt ttggttctac agaagtaaat tgggggttta cttcttcaac aggtactttt
     2101 tttcaagaaa atgctgtcgc tgtcacgcga cttccatcga atgtcacatc aacagctgat
     2161 gtaaaagtca acgatagtga ttatggggcc atcgctgaag ctggaggaaa cgataagata
     2221 actatcagaa atacattgaa ggtcgagggg gattttcttg aatataagaa aatgcagact
     2281 agtatcgacc tcccacaaga attaactatt cccgagcata tcttgatcaa tggaaatcaa
     2341 attgatgcca aagatatctt gataaccgga aatcgagtca cgctaaatct gacagattat
     2401 tttattgctg gagatacgtt aataatcgaa ttggagacaa ctttgcagga agtaattcct
     2461 gaaaaagtac tcactatgca ttttgaatac cttgaaaacg ggaatttgat tgagcaatcg
     2521 aatgaaataa acctaaagat cgcaaagctg aaggagatag atatccatgt ttattataaa
     2581 gataaagaca gtcagattga tattgctgaa atgaaaacaa tcactgggaa aataggagag
     2641 aagtataaag aaactcctat tgatattgaa gggtatgttt ttgactcgga ttcagcgaat
     2701 acagaaggca tcttctccga aaatacagag gatatctatt tttattacag aaaaggcaaa
     2761 ctttatctct caactgctcc tcaatatgtc aattttggaa aacataaaat atccagtaga
     2821 ccattaagtg aattcggaca gttttcaggt gggttgaaaa ttatagatga acgggctttt
     2881 ggaagttgga aattacaatt gaaacaagag aaagttctta caaatggaga ttttgagctt
     2941 ccagaagctc tttcctttgt tacctctgaa aatacaacgg ttatagggac atccgctgtt
     3001 acgattttcg aaagtgatca aaagggtgaa agcgatttgt cctctctttt agattctaca
     3061 gctcatcgtg gaatcagaat agatattcct gtagaaaacc aaagaatcgg aacctttgag
     3121 gggaaactat cttggatact tgcagatgtc ccaggaaatt aacaggaaga gaagaatata
     3181 acaaaattat tgagtttcaa gaagggcaca ggtaccatag gctcttgtgt cccacagaga
     3241 caagattttg ttatagtctt ctcttacata tgattaaagg agcgattttc ttgagaccaa
     3301 aacaacaatt atttataaca ctcctagtta ttttaggggc actttttaac aatatatcag
     3361 tgtcagctag tgcatttaat ttttcagtga cgccgataac ttcagagaat caaatcgaca
     3421 agagaaaaac ttacttcgat cttcaacttg atccggatca ggaagtagaa gtaaaagcgg
     3481 aattgagaaa tgatacagaa aaagaagtga aaatcgatat ttctgtgaat agcgcaacca
     3541 ccaattcaaa tgtgatggtt gaatacggaa aaaacgagat tgaaaaagac atgagcctga
     3601 tctttgatct ggtagattat gtgagctatc cacagatagt gacgctaaaa cctaaaagtg
     3661 tccaaaccgt tgcttttcat gtcaggatgc cgaatgagcg atttgatggt gtattggctg
     3721 gtgggatcac attccaagaa atccagacgg aaaaggacca aacagaaact aaggatcaaa
     3781 gcttatcgat tgagaatgaa tacacctata ttattgcttt gttgatgcaa cagacactga
     3841 acgaagttgc tccgaacctt actcttcatg aagttaaacc agatcaaatc aatgcaagaa
     3901 atgtcatctt agcaaacgtt caaaatgatc aaaaaaccta tatcaatcaa gtagttattg
     3961 aaacaaaaat tacaaaaaaa ggacattctg aagttttata tcaagaggaa aaagaaggat
     4021 tgcagattgc gccgaataca aatttttctt ttccaactgc tttaaatggt caaccgctaa
     4081 ccccaggtga atatcacctt acaatgactg ttttaggaaa tgaaaatgaa aatggaaaat
     4141 tttcgagaaa aaaaggaaat actacgatca actataccaa tcaatgggta tttgaaaaag
     4201 attttacaat agacggaaaa gtagccaagg aattgaacac gacggatgtt acgattaaaa
     4261 aagacaataa ttggatttat atcttagtag cttcactttt cttattgttt attttattat
     4321 gcatactttt gtttgttttg agaaaaaaag aagagaaaga aaagtaaggt taagataaca
     4381 aatagtaaat ttttattatt gtaggatggt t
//
LOCUS       DAZULY010000206         4396 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000206 DAZULY010000000
VERSION     DAZULY010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.208"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..93
                     /locus_tag="ACG81A_002373"
     CDS             <1..93
                     /locus_tag="ACG81A_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFS5015697.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(151..1371)
                     /locus_tag="ACG81A_002374"
     CDS             complement(151..1371)
                     /locus_tag="ACG81A_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFS5015698.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1391..2002)
                     /locus_tag="ACG81A_002375"
     CDS             complement(1391..2002)
                     /locus_tag="ACG81A_002375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFS5015699.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(2045..2977)
                     /locus_tag="ACG81A_002376"
     CDS             complement(2045..2977)
                     /locus_tag="ACG81A_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFS5015700.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3398..4258)
                     /locus_tag="ACG81A_002377"
     CDS             complement(3398..4258)
                     /locus_tag="ACG81A_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5015701.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1257 a          882 c          727 g         1530 t
ORIGIN      
        1 cgaggattat ataagaaaac ccgaaaagaa ggcactctct tcgggttttc ggtctgtact
       61 gaaatcaagg tattattggg aatcccagct taaatcatag ataccgtaag ggactttatt
      121 ctttatttaa aactttgcaa cagaaccttt ttattttaca gaagcactcg aaactgcatc
      181 tactgcagtt tcatcgtgtg tgctggcgct ggttactgca tctgtttttc caccatgaac
      241 agatgcactt gaaaccgcat cttcatttcc aacaagttgt tgacccgttt gttgtaaata
      301 ccactcaatg attggtttga aatcaagaat gtattcattg attccagcat actctccatt
      361 ttcatcatgc attgcttggt aattatgcac aatatatttt tctggcactt gttttggtac
      421 atgaacacgt accgcatctg ttgctccgct tcgcagttgt tgaattaccc actctacatt
      481 tttatacgat ttttctgggt gacagttggc taatggattc cctacctgac cgggcacacg
      541 agcagctaac atctctttcg cttcttggcg caaattataa tataaaaatt ggttattatt
      601 atctgcaaaa gttaattcca tcggcatcga atctaaaaag taattgattt gatccactgt
      661 caataaccca cgatttaatt tgacgtaagt atttccagaa acggcaccga ctgcttcaga
      721 agctttttcc aaccaatgat catctgacat atcaacatcg ggtattgtcg tagcaattgt
      781 tcctgtcgca tgcaaatctt ctggtggaag ctttttaggc cctgctaagg tagacacgac
      841 tttaatgaat tttacaaaac ttgctaatgt actgcctaaa aagtcaacag ttcgtggatc
      901 aatcaagcga tcttcttggt caaatgcttc ttttgctttt cccaataaaa attcatttcc
      961 gggcatcact aatgcattca cgcctggtgc gtccaaaatt tggcgcaagt gtaattgcgc
     1021 gcgtgaagtt ccttgatcgt agtaagaagc acccacaacc attacaggtt tattttcaag
     1081 tggatggatt ttaaatgata gccattcaag tgcacttttc aatcctgctg gaaccgtatg
     1141 attgtgttct ggcgtagcaa taattacccc atcagccatt aagattttgc gatttagctg
     1201 ttgaatagca ggactatttg tttgatcatc atcttgattg aacagaggta aatctttgat
     1261 ttcaatgatt tctaaattaa atagttcatg atattgtttt tggataaatg ttaataattt
     1321 acggttatat gaaagttcag ctgctgatcc gacgatacct attaatttca tctttgtttg
     1381 ctcccctcga ttaaatcgtt tcccatgaaa agctttcggc ttcacgttta tttgcttggt
     1441 gcgcatgttg caattgacta gtgattgtga caaagagtaa gaaatctttg aatagttcac
     1501 gtaattttgc gacttgttct tcatcaatga gtcgtccttg ctcatcaaag gcttggagtg
     1561 aatgacccaa taaaaattct gagcttggca taattcgtgc ctttaactct ggtgaatcca
     1621 atatttggcg caaatgtgct tgtgcgcgtg aagaacctaa cgtgccataa gacgctccgg
     1681 taatcatcac tggtttatcc acaaacggaa aaataccata tgaaagccaa tttaatgcgt
     1741 taatcaatga agcaggaacc gcatggtcat actctggtgt gctgatgatt acgccatctg
     1801 cttgttcaat ttttccagca atttctttaa caacatttgg tagttctgtg ttttcaggtt
     1861 tgttaaatag tggcagctcc ttgatttcga caatttcgat ttctgcctca tttacaaaat
     1921 aatttttcat aaactgtaat aattcacggt ttgttgaccg cgttgaattt gttcctacta
     1981 atccaataaa tttcatcttc attctctttc ctttctaaca tcgatttctg actgttcgca
     2041 cgtactaaat tttttcaaat ccttttgtat acaagaattg attctctttt gttacaactg
     2101 ctccctcgat acccggctgt tcttcaagcg ctttcaacac ttctaaagaa gatgctccaa
     2161 ataaacgtgt agtccagatt tctccatcta cagaagaatc agacaaaatc gtcaggcttg
     2221 ctacgtcatt tttaactgga aagccagttt tcggatcaat gatgtgatga tacactgacg
     2281 aaccattttg tagataacgt tcgtagattc cagaggtaac taaagattgg ttgtttagtt
     2341 taaccaccgc aagcgactct tcacgtgaac ctttaggatt tcgcaaacca atacgccaag
     2401 caccatccga acgtcttggt gattcaccaa aaacaacgat atttcctcct aagttaacta
     2461 aagcaaacgc tgcatttcgc tctcggcaat gtgtaattaa tttatctgca atgtaacctt
     2521 ttgctaacgc ccccaagtca atcatcattc ctggtttggt taaaaaaacg gttttcattt
     2581 tttcatctaa ttgaatttgg tgtggatccg ttagcagaag taactcagtt atctctgctt
     2641 ttgttggaac caatgcatct tcaaatccaa tgtgccattt ttgtacaacc ggaccaatag
     2701 caatattcag ataactattt cttgcacaac tatgtgcttt tcctaatttg atgagctcaa
     2761 aaagttcagg atgtacaatc acagcttgtc gtccagcagc ttgattgatg gccattaatt
     2821 cagaagttgc atcatgtgcg ctgaagcgat gttcataaac gtgcaaccat tcgcctaact
     2881 ctgctagtaa tcgactaggc tcttcatgcc caacttgtaa tgtaattgtt gtccccatca
     2941 tttgccagat tttcgtttct tcgatcacct gattcatttt gtaccttctt tacttgaact
     3001 acggattaca tgagtaatta gtttttattt ttgttactgt tttcacaacc agatgatacg
     3061 cctgctagat ttatttgtca accgatttta tatatttttt tatataataa ggtaatttta
     3121 atataaaatt ttatattgta aaaaaacaaa taagggaaac aaacaacaca tttttgtgtg
     3181 ttgcttattt ccctctaact atcgttttct atttgatggt taacctactt aaaataaata
     3241 ctgcaacgac aaacggagat caagcatgct ttgtaacgta ttttacgata attacgactt
     3301 tattttatta ctctatcttg ctgccatacc cgtctatccc taatcatctt tggtgaattt
     3361 tttcaaagtt agcataacaa aaataagtat tttttcctta gttaatcaat agtatcattt
     3421 ttttcatatg atttaattca tcaattaatt ttttgttttt attaatttta gcaaaagcta
     3481 gtgcatcttg aatcaattcg agcacttctg ccttcgactt atttttagcc tgtgcgttct
     3541 tcgctaattg ggcagcagct cgtgctaagt aataagtcaa atggttatcc gaacaaatat
     3601 cgattgcgta atttagtagt gcatcacttg tttccaaatc accgtcttgc gcaaaaaaaa
     3661 caccacaatg aaagacgata tttaacaccc gccagatatc ttctgttgta cgaatcggat
     3721 acgtataaat tttttcaaac actcgattga aataaacttc tgctttttcc aactcttttt
     3781 ctctagcata gaccattcca atacctgtat atgccaataa acgaaacaac gtgttggctg
     3841 tttctgccat caacaactga tcaaaatgga acaacacatc cgtaattggt gcattcgtaa
     3901 aaatcatttc aaatcctttt agatagtagt aacgtgcaat cagctcagac tcgtcaatta
     3961 ccttgggatc aatggctgct aaaatctgtg tcaattgttc atattcactc gtaatcagtg
     4021 aaaactctgc tttttctagc tgttcatcaa gttgtgtatt gatcacgttc atcgaagaaa
     4081 aaagttcaac taacggaaga ttgattcgat tacataattt gattaaaatt tttaacgaag
     4141 ggacttgacc attgttctca aatttactta atgttgcttg tgtacaaatt ccttcagaca
     4201 cttctttttg agataagttg gcagcctttc ttgccgtaat aaatttttca atgttcatta
     4261 ttttctctcc aatcagacct cacacgaaaa tcatttctgt ctcgatttta gcatgttttc
     4321 tttgggaatg ctttaggttc tgttgcaaag ttttaaataa agaataaagt cccttacggt
     4381 atctatgatt taagct
//
LOCUS       DAZULY010000207         4384 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000207 DAZULY010000000
VERSION     DAZULY010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.209"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..538)
                     /locus_tag="ACG81A_002378"
     CDS             complement(17..538)
                     /locus_tag="ACG81A_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFS5015702.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            737..>4384
                     /gene="nifJ"
                     /locus_tag="ACG81A_002379"
     CDS             737..>4384
                     /gene="nifJ"
                     /locus_tag="ACG81A_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFS5015703.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAER"
BASE COUNT         1375 a          826 c          971 g         1212 t
ORIGIN      
        1 cacgctcctt atttttttat tttgtttgga tcaattcttt taattcctct attgaaatag
       61 attcatcaaa gatttcttta cccaagatga cagttggaac aaatcgaata tgtgcttgct
      121 cagcttcgtt gatgacagct tggctcagct gttcgtcttt ttgttctgtc agctttagtt
      181 tatctactgc aaaatccgct acttcttgga gagaaagatg tttccactcg tcttgagtat
      241 cgaatatttg tttgatctct ttgatcgctt tttggccatc gctgattgtg agataccggt
      301 gcatcacgtt ccctcgctgt aagctttctt tttctttatc gaacaatttg atcactcgct
      361 gcaattgccc gctttggact gcttcttcta atagctcata agattcttca aaccattgtc
      421 ggcaatacgg acaagctaag ttgatgaatt cgaccatttt tttaggagca ttcgatgaac
      481 cataaaggat tcctttttgt gtatttgttt tcgttgcatc aatcactgat atatccatgt
      541 taaaacacct ctttcgctta tagtttatac atgtctgaca aaaaaaggca acgatttaga
      601 ctgtttgtct aaaaattaga tgaccaattc aaatagatac atgtaaaata tttttataaa
      661 taaaagaaaa aaatggcatg ttttacgtta taatggaaca gataataaga gagaatatga
      721 acaaggatga tgaatcatgc gaaaaatgaa aacaatggat ggcaatacgg cagccgcata
      781 tatttcttat gcatttaccg agattgctgc aatatatcca ataacaccca gttcgaccat
      841 ggctgaatta gtagatcaat ggtcagcagc aggaaaagaa aacgtattcg gtcaaccagt
      901 aaaagtagta gaaatgcaat ccgaagcagg agcagctggt gtggtccatg gatcattgaa
      961 aactggtgca ctgacaacta catataccgc ttcacaaggg ctgcttttga tgataccgaa
     1021 tatgtacaag attgctgggg aactactgcc aagtgttttc cacgttgcta gtcgggcagt
     1081 gacgacaaat gcgttgaata ttttcggaga tcacggagac gtgatggctg caagacaaac
     1141 aggatttgct atgttagctg aaagcagtgt acaagaagtg atggatttag cacctgttgc
     1201 ccatcttgct tctattgaag gagaagtccc ttttttgaat ttctttgatg gattccgtac
     1261 gagtcacgag attcaaaaaa tcgaagtttt ggaatatgaa gaattagctc ctctgatcaa
     1321 tcaggagaaa ttagcttcat ttcgtaaacg agggatgaat cccaatcatc cgactgttag
     1381 tggtacgaac cagaatccag acatccattt tcaacaacgg gaaacgatca acccctatta
     1441 tgataaatta cctgaaatcg ttcaaaaata tatgaaagag atcaaccgat tacgaggaac
     1501 ttcttatgac ttggtgactt attacggagc agacgatgcg gaagaagtca ttgtttctat
     1561 gggatcagtt gcacagacga ttgaacaaac gattgattac ttgaatgcaa acggacggaa
     1621 aacaggtttt ttgaatatcc atctttatcg tccttttcca gtggaaacat tcgtgcaaaa
     1681 gttgcctcat acagtaaaaa caattgctgt tcttgatcga agtaaagaac caggggcagg
     1741 tggagaacct ctcttattag acgttcaaag tgcgttatat gattctgaac ttcgcccggc
     1801 tgttattggt ggtcgctacg gattggggtc taaagatgtg actcccgacc aaatctcagc
     1861 agtttttgat gaattgaaaa aagacccaag catacgcaaa aaacggttta ccatagggat
     1921 cgtggatgat gtgacttatc aatctttgga accaaaggaa agtctggatt tgacagaacc
     1981 gcaaacattc caggccaagt tttggggatt tggatctgac ggaacagttg gagcaaataa
     2041 atcagctatc aagattattg gagatcacac cgataaatat gcgcaaggct atttttatta
     2101 tgattcgaaa aaatctggcg gtctgactgt ctctcatctt cgttttggag atacgccgat
     2161 acgttcagcg tatcttgtcg agcatgcgga ccttgttgcc tgtcacacac cagcttacct
     2221 gcatagctat gacttggtca aaggattgaa gccaggtggc atttttttat tgaatactct
     2281 ttggagcgac gaacaattag aaacgcatct gcccttaaaa ttaaaacgtt acttggctga
     2341 aaataacatt agattctata cgatcaatgc catgagattg gcccaagaag tcggtttggg
     2401 acgccggatc aatacggcaa tggaaacggc tttttttaaa ttagctgaca tcattccttt
     2461 tgatgaggtg cttccgctgc taaaagaaga agccctgaaa agctatggac ataaatcaat
     2521 ggctatcgta gaaaaaaaca tacaagcaat cgatcggacg accgaactgc ttcatcaagt
     2581 cgcgatccca gaccattgga gaacgttgga gatgcctgaa aaaatcaata aaaggaacac
     2641 tggttatata caagaaatac ttgaaccagt gaatgcacag gaaggaaatc aactctctgt
     2701 tggtacactt gtagcaaatg gaatgacaac tggcgaaatg cctttaggaa cggcttctat
     2761 ggaaaagcgg ggcgtcgctt tagaagtacc tgagtggatt agtgatcgct gtacgatgtg
     2821 taatgaatgt gcctttgttt gtcctcatgc tgccatccgt ccatttttag cagatgaaga
     2881 ggaaatggaa gaagcgccag aaggatttat cgtgagagaa atgcgaggcg cagacggcgt
     2941 aaaataccgt atccaagttt cagtggaaga ctgtacaggt tgtggtctgt gtgtagaggc
     3001 ttgtcccgca aaagaaaaag ctttagtgat gaagccttat gaagaacaga aagaacaagc
     3061 tgtgaattgg gcttttgcaa tgacattaag acaaaaagca aatccagtca aaggaaagaa
     3121 tactgtcttg tctacacaat tcaatcaacc gcttcttgaa ttctcgggcg cttgctcagg
     3181 ctgtggcgaa acaccttatg taaaattatt gacacaaatg ttcggtgatc gaatgatgat
     3241 cgcgaatgcg acaggctgtt cttctatttg gggcggtgct tctcctgctt ctccttatac
     3301 gaccaatgaa tgtggtcaag ggcctgcatg gtcaaattct ttattggaag ataatgctga
     3361 atttggttat ggtatgcttc tagcggcaca gactcgtcgc gaggctttgg caagttcaat
     3421 caaagaagta ttgccaaaag tatcagaaga actacgagaa ttgatggaag actggttagt
     3481 acacatgttt gagagtgaag gaacgcagca aagagcagca aaactgcaag ctgcgatcga
     3541 aagcgaacga tcaattttcc ctgaactgga ggatatatac aagagaaagg atctattcgt
     3601 aaaaaacagc caatggatga ttggtggaga cggatgggct tacgacattg gttttggcgg
     3661 gatcgatcat gtacttgcca gcggagcaga cgtgaatatc cttgttttgg ataacgaagt
     3721 ctattccaat actggcggtc aaacctcaaa agcaacacca gcttcggcaa tcgctaaatt
     3781 ttcggctagt ggaaagtatg cttcaaagaa agatctagga atgatggcga tgacatatgg
     3841 aaatgtttat gtggcgcaaa ttgcttcagg tgccaatcaa atgcaaacga tcaaggcatt
     3901 tgaagaagca gaaaagtatc ctggtccttc tctgattatt gcctatacgc cttgtatcac
     3961 acatggcttg cttggcggaa tgaaagaatc gcttaaagaa gcaaaagaag ctgtttcttc
     4021 tggttattgg tcattatatc gatataaccc tacaatggtc gaaaaaaaca agaacccaat
     4081 gactttggac tacaaaaaac ctgatttctc tgctatgcct gatttcatga ggaaacaagt
     4141 tcgcttttcc tcgctagata acgcaaatcc aaatcttgca aaagctttat ttgaaaagac
     4201 tgtccacgac gctaaaacac gtttttacaa ctatgctgca ctgacgggag aattagaaaa
     4261 aatcaagaaa cgacttgaac cagaggaatc tatcgatgta cccgttaaga ctagaaaaga
     4321 aaaaacgacg gaccttgaag cagaagaaag aagagctgct agaagagctg ccagagcaga
     4381 acga
//
LOCUS       DAZULY010000208         4349 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000208 DAZULY010000000
VERSION     DAZULY010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.210"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      24..190
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            338..895
                     /locus_tag="ACG81A_002380"
                     /pseudo
     CDS             338..895
                     /locus_tag="ACG81A_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            953..2992
                     /locus_tag="ACG81A_002381"
     CDS             953..2992
                     /locus_tag="ACG81A_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFS5015704.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3101..4252)
                     /locus_tag="ACG81A_002382"
     CDS             complement(3101..4252)
                     /locus_tag="ACG81A_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFS5015705.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
BASE COUNT         1577 a          804 c          855 g         1113 t
ORIGIN      
        1 atgaactcgt aaatagaata tagtaataac cttgttaggt gaggctccta tacaaacata
       61 ggctgttgcc cagaaacgtc gaaagtcgcc aatgggtaaa acagaaactg ttggattaag
      121 gctttttcca atagctaaga atgttttctt tacgttgtat agtgctaaaa ctcggacgat
      181 gagatgatgc taggtcagaa ttcttgttcg aaaaggtttc tgaccttttc tttatatttt
      241 tgtgactgac ttatttgatg agacaaactc gctcctcacc tgacaagtgg attgctgtgt
      301 gaggaggaga tgcggtgaaa aaagatcgat tggtggaggt cgctcggatt cacttctggt
      361 tgcacaatac aaccagaaaa cacaacaagc aaaactgatc tctatcatga gagattcata
      421 tgtcgatatt ccaggttacg gaatgaataa gataaatgca gcttactctt atgggggagt
      481 tgatttatta aatcaaacat taaaggaaaa tttcaaattt gaagctccct attatgcaag
      541 tatcacattt caagatttta tcgattgcgt taatgaactg tttcctggtg gagtcaagat
      601 cgatgcagaa aaatctctag atttagatgg cgtatatata aacaaaggag agcaagtgat
      661 ggatggtaat actttactcc agtatgctcg atttcgggaa gacaaagaag gagacttcgg
      721 gaggatcaga aggcagcagc aagtgatcaa agctatctct cagcagctga aagacgtaac
      781 ttcaatattc aagctaccca aagcggttgg gaaattactt ggtagtatcc aaacgaacct
      841 tccagaaagt gtgcttctgg attgtggtat ggatttcctc aaagacatat gttagaataa
      901 acaggtataa atagtgaaat aaaggaatga caagcaagag aaggaggaaa aaatgaaaag
      961 aagtgacaag cacggcaaaa atcgaacagg cgcttatatt gccggcgcag tgatcttaat
     1021 agcagctgcg ggtggcggtt atttttacta ccagcactat caagaaaccc aagcagtaga
     1081 agctggagaa aagacagttg agcaatttgt ccaagctttg aacaagggag attataacaa
     1141 agctgcagaa atgacatcaa aaaaggcggc aaataaaagt gcattatctg aaaaagagat
     1201 cttagataaa taccagaata tatacggtgc tgctgatgtc aaaggacttc agatatcgaa
     1261 tctaaaagta gataaaaaag acgattctac ttacagtttt tcatataaag caaagatgaa
     1321 tacctcatta ggtgaattga aagatctttc ttataaagga acattagaca gaaatgatgg
     1381 tcaaaccacg atcaactggc aacccaactt ggtttttcca gaaatggaag gaaatgacaa
     1441 agtaagcctg accacacaag aagcagcaag agggaatatt atagatcgaa atggtgaacc
     1501 attagcaaca acaggcaaac taaaacaatt aggagtcgtt ccaagtaaac ttggagatgg
     1561 aggcgaaaaa acagccaata tcaaagcgat tgcttcctcc ttcgacttaa cagaagatgc
     1621 tatcaatcag gcgatttcac aaagctgggt acaacccgat tactttgtcc cattgaaaat
     1681 cattgatgga gcaacgccag aacttccagc tggagctacc attcaagaag tagacggcag
     1741 atattatcct ttgggtgaag cagctgctca gctgattggt tacgtgggag atatcacagc
     1801 agaagatatt gataagaatc cagaattaag cagtaatgga aaaatcggtc gctcaggttt
     1861 ggagatggct tttgataagg atcttcgtgg gacaacaggt gggaaattaa gcattacaga
     1921 tgcagacggt gtagagaaaa aagttctgat cgaacatgaa gttcaaaatg gcaaagatat
     1981 caaattgaca atcgatgcga aggcacaaaa aatagctttt gacagtctag gaggaaaagc
     2041 tgcatcaact gttgcgacaa cgccaaaaac cggtgatctt cttgcgcttg ctagctctcc
     2101 aagctatgat ccaaacaaaa tgacaaacgg gatctcacaa gaagattaca aagcttatga
     2161 agaaaatcct gaacatccat tcatcagccg atttgcgaca ggttatgctc ctggatctac
     2221 gtttaaaatg attacagcag caatcggtct cgacaacggc actatcgatc caaatgaagt
     2281 gttgacgatc aacgggctta aatggcaaaa agacagttct tggggatctt ttaaaacaac
     2341 gcgtgtcagt agtgatgtat cacaagtaga cttaaaaact gctttgatct attccgataa
     2401 tatatatgcg gcacaagaaa cgttgaaaat gggtgagaaa aaatttcgta caggcttaga
     2461 taaattcatt tttggtgaag accttgattt gccaattagt atgaatccag cacaaatttc
     2521 taatgaagat agctttaact cagatatctt gctagctgat actggatatg gacagggcga
     2581 acttctaatt actcctatcc agcaagcagc aatgtattct gtttttacca acaatggcac
     2641 acttgtctat cctaaattga ttgcagataa agagacaaaa gataagaaga atgtcatcag
     2701 cgaaacagca gtacaaacga tcgtgccaga tctgagagaa gttgtgcaag atgtaaatgg
     2761 tacagcacat tctctttctg ctttagggat tccgttggca gcgaaaactg gtacagcgga
     2821 aatcaaagaa aaacaggatg taaaggggca agagaacagt ttcttgtttg ctttcaatct
     2881 tgataaccaa ggttatatga tggtcagcat gttggaaaat aaagaagatg atgattcagc
     2941 aaccaaacga gcatcggaac tattgcaata cctcaaccaa aattatcaat aaaaaattgc
     3001 tttttaagta cagtaaaagg actgtggcgt tatcgtcaca gtcctttttt caaataaacg
     3061 gttttcacga tgtctctttc acaacttctt tttgatttac tcacacttca taaacttttt
     3121 ttgtaaaata tttgagaaca agtggagaaa taagtgtact taataaaatc acgataacta
     3181 aaggtgaata gtactgtggt tcgatcaatt gactttgctg tccaatttgt aaaatgatca
     3241 aagccatttc tccacgcgaa atcataccag caccgaccat caatgaacta tgttgagaaa
     3301 aaccggcaat ctgagcacca aaataacctc caagcaattt ggtcaaaatc gctacaagcg
     3361 tcaatataga aataaagagt agttgttcac taaagtcaga aaaatcaact tctaatccta
     3421 cactgacaaa aaatactggt ataaatactg catatcctaa agcctctaca ttgtggtata
     3481 cttcttcttt aacctttgtc tgactgacag ctatcccagc aaaaaaagca ccaatgacag
     3541 agctaagccc aacaagatcc gctacgtaag acattcctaa acaaataact agcgatacga
     3601 tgataataga agaatttgcg tacacttttt ctgcaaatga aagtaggaaa ggcgcaatcc
     3661 atttgactaa taagaaaata aaaacaaaat aaattagctg ttcaatcaaa acaagcggta
     3721 aagaagcatt gctagaagct tcccccgtta gaaaagatag gctgaaactt agaactaaaa
     3781 cgactaaaat gtcatctacc acagaagcac ccaaaattgt tgatccttct tttgtattca
     3841 ctacgtttag ttcttttaat acttctactg aaatactgac agaagttgcc gcaagaataa
     3901 tcccaaagaa aatcgcttca tttcccgaaa cttggaatgc tttgcctgtc aaccagccaa
     3961 aaacaaccgg gaagatgatc ccaagcaaag caacgaacat accaggtcgg aaatattttt
     4021 tcaaaagaga taaatcactt tccagaccag ctaaaaacat taataagatc acaccaatct
     4081 ccgaaaagtc atggactaga atatcaggtt tgacccaatt caatccagct tggcctagca
     4141 aaacaccaac aagtaattgt ccaatcactg ctgggatacc taatctccgc gagatatgtg
     4201 agccaatcgt tgttgaaatc aatatcaggc ataaaatacc aataaattcc attctcatca
     4261 ttcctttgta taaaatatag cccgaactta gcataacata cattattgaa aaaataggaa
     4321 aatatactca caaaaaaacc aacaatcca
//
LOCUS       DAZULY010000209         4344 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000209 DAZULY010000000
VERSION     DAZULY010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.211"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..1741
                     /locus_tag="ACG81A_002383"
     CDS             110..1741
                     /locus_tag="ACG81A_002383"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS5015706.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1744..2823
                     /gene="uxuA"
                     /locus_tag="ACG81A_002384"
     CDS             1744..2823
                     /gene="uxuA"
                     /locus_tag="ACG81A_002384"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS5015707.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2897..4324
                     /gene="uxaC"
                     /locus_tag="ACG81A_002385"
     CDS             2897..4324
                     /gene="uxaC"
                     /locus_tag="ACG81A_002385"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS5015708.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
BASE COUNT         1504 a          608 c          909 g         1323 t
ORIGIN      
        1 acatggtaga aaaaagaata tttttagttt tttcttattg acagcgatta cattcgattt
       61 tatactacaa ttgtaacgga aactaacata tcagtatgga ggggtaaata tggttttgat
      121 taaagactgg caagattcta tcgaaggatt gaacaaaatg caagttcaac taccgtattt
      181 taatgtcgaa gcggtcaaac aagaaggtca gaaacatcca gtgtggcttc attttggtgg
      241 aggaaatctt tatcgaggat ttcatgcaga aattgctcaa tgcttagcag atcaaaatga
      301 attagaatcc ggagtagttg tgtgtgagac atttgatgaa gaagtagtag aaaaagctta
      361 tcatgactac aacaatgata ttttggagat tgttatgggt gaagatggtg aactcaaaaa
      421 aagactttta gcagtaacag ctgatagttt atattgcaat ccaaagaata taacggacta
      481 tcaaagagtg gttaaatatt ttgaagatcc tacattacag tttacaacgt ttactattac
      541 tgaaaaagga tacagtttaa aagattctaa aggacaattt ttttcggtag tggaagcaga
      601 tataaaatct ggacctacca atcctcaaca tacgatgagt attgtgacat ccttgttgta
      661 tagcaggttt aatgctggag agttaccgat tgcgatggtt tctactgata atttctcaca
      721 gaatggtcaa cgttttcaag atagcatttt tatgattgca gaagcttggc aaaaaaaagg
      781 atacgtttct gaaaaattta ttgcttattt aaaggataaa gaaaaagtga cttttccttg
      841 gagtatgatt gatagaatta cgccaaatcc ttcggaaagt gtcaaaatgg atttattgaa
      901 ggcgggtttc gaagacttag atataattca tactgcgaaa aataccaata tcgctgcttt
      961 taccaatact gaaaaagtcc attatctagt cattgaagat agttttccaa atggtcgacc
     1021 taaactggaa gcggctggtg tgattatgac agatagagaa acagttgata aagcagatat
     1081 catgaaggtt acgacatgtt taaatccgct ccatacggca ttagcaataa caggttgttt
     1141 gttggggtat aaatcaattc aggcagaaat gcaagatgat gatttggttg gcttaattaa
     1201 agggataggt tatgtagaag gcttaccagt agttgaaagt ccaggcgtta ttgatccaaa
     1261 acaatttatt gatgaagtaa tcaataaacg tttgcctaat ccctatattc cagatacacc
     1321 gcaacgaatt gcaacagata cttctcaaaa agtagcgatt cgctatggtg aaacaattaa
     1381 aaagtatgtc gaaaaagacg gaaatgcaga aaatctaaca tttattccta tcgctattgc
     1441 tgcttggtta cgttatttat tggcagtcga tgatcaagga aaaaattttg tgccaagtcc
     1501 tgatccttta ttggtagagt tgcaggaaaa attctcagta ttgaaattag gtgaacaaga
     1561 tcctgcaaag attcattgtg cggtacagga aatcttagaa aataaggaaa tatttggttt
     1621 agatttatat caagttgaga taggaaagaa aattgaaaaa atcttttgcg aaatgctagt
     1681 aggtcctcaa gcagttcgta aaacaatcaa taaatatata acagaagttg gaggaaagta
     1741 actatggaaa tgacttttag atggtatggg gcaaatgaag aaaaaatctc actacaagaa
     1801 attcgtcaga tcccagcaat gaaaggaatt gttggaacat taatggatat accagccgga
     1861 gaggtctggc cgagagaacg aattaaagct ttaaaagatg aagtcgaagc caatggcctt
     1921 accttgaaag ttattgagag tgttaatgtt catgatgata tcaaaattgg tttgccaaca
     1981 cgagatcaat acatcgagaa ttataaacag acgattcgca atttggcaga atttgggatt
     2041 gaggttatct gttataattt tatgccagtc tttgattggg taaaatcaga tttggattat
     2101 catttagatg atgggtcttc tacgttagcc tatatttcaa aaaatattcc tactgatcca
     2161 gaggaaatta ttagaaaagt agaaaattct actaataatt ttgaattgcc gggttgggaa
     2221 cctcatcggt tatcacaaat caagcaatta ttagaagcct ataaagaagt tgatgaagcg
     2281 aaattgcgag agaactttgc atattttctc aaagcaatta tcccgacatg tgaagaagtt
     2341 gggattaaaa tggcagttca tccagatgat ccaccatatt ctattttcgg attacctcgt
     2401 attgttaaaa atcgtgacga tttagaatgg ttgtgtaatg tagtggattc tccttctaat
     2461 ggtattacgt tatgtactgg aagtattgca gaacgaccag ataatgatgt ctatgaaatt
     2521 ctatctgaat tttgtcaacg ggatcggatt ccttttgcac atgttcgtaa tatcaaatat
     2581 ttaagtgata atggactcga tttttatgaa gcgccgcata aatcagaata tggttctcta
     2641 gatatgtata agatattaaa aacaatgtac gataatggat ttaatggcta tattcgtcca
     2701 gatcatggtc ggatgatttg gggagaaacc ggtagacctg gttatgggtt atatgaccga
     2761 gctctaggtg cagtttattt aaatggtatt tgggaagcgc ttgaaaaggg ggcgagtaaa
     2821 taattatata atggagtcta tttagaagaa aaattttctc ttctaaaaac taagggaagg
     2881 aaaggagtgc ttaaatatgt ttttagataa gaacttttta ttagagaatg aaatagctaa
     2941 aagacttttt cataaatttg cagaacacca accaatcatc gattatcatt gtcatttaga
     3001 cccagcatgt ttgctagaaa ataagcaata caagaatttg actcaggtat ggttaaatga
     3061 taaaggagct ggagatcatt ataaatggcg attgatgcga gctaatggga tagaagaaga
     3121 gttgatcagt ggtgcaggta atgattatga aaaattttta gcttttgtaa aaacagtgga
     3181 gaaggcgcca ggtaatccta tttttgaatg gtctcatttg gaattacaac gttactttga
     3241 tattgatttg acaatttctg agaagaatgc accagaaatt tggcgacgtg ccaacgaaaa
     3301 aatccaaacg gattcctatc gcccaaaaga attgctgaag atgatggatg tgaaagccat
     3361 ttgtacaacg gatgatccta cagatgactt gcgatatcat actgagcttt ctaaacagga
     3421 taattctttc aaggtattac ctactttccg tccagataag gtctggagta tcaaatcatc
     3481 aggattttcg aactatattt taaaacttag tcaagcagcg ggtataccaa tcaaaaactt
     3541 taatgggttg aagcaggcat tgagccaacg agtggattat tttcatcaaa ttggctgtcg
     3601 attagcagat cacggtttga atacttattt ttatgaaata gcagatgaaa aaatactcga
     3661 caatctgtta ataaaagctt tagcaggaaa aacggatttc catttatcag aaattgcaca
     3721 atttgttaca ggtattcaat tacatttgat gtctgagtat acaagatatg gttggacgat
     3781 gcaaatgcat ttaaatgctt taagaaataa ttcaagtata ttaaaggaac aaattggagt
     3841 agatgtcggt ggtgattcga tgggggatca agctagtttg acacaaaatg ttgtagcatt
     3901 gttcgacact gctgaaaaga gtgggtgctt accaaaaaca attttatatt cattaaatcc
     3961 aaccgattgg cttccactag caacagcaat gcaaagcttc caagggggta tgattcaaaa
     4021 aatccaattg ggatgtgctt ggtggttcaa tgatacagct gaaggaatgc gtcaacagtt
     4081 gactacgatg gcgcaacaaa gtttgttgtc gaattttaca ggtatgttga ctgattctcg
     4141 aagttttcta agctatcctc gccatgagta tttccgtcga gtattatgtc aattactaag
     4201 cgagtgggtt aaagcaggtc gaattccaga tgatgaaatg tatcttagtc agatagtgga
     4261 gaatatttgt tatcgtaatg cgtataatta ttttaatttt tttgaaatcg aaagtagaaa
     4321 ataatacaaa aaataaaagc ttaa
//
LOCUS       DAZULY010000210         4326 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000210 DAZULY010000000
VERSION     DAZULY010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.213"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..283)
                     /locus_tag="ACG81A_002386"
     CDS             complement(<1..283)
                     /locus_tag="ACG81A_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFS5015709.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENI"
     gene            complement(732..2140)
                     /locus_tag="ACG81A_002387"
                     /pseudo
     CDS             complement(732..2140)
                     /locus_tag="ACG81A_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2361..2723)
                     /locus_tag="ACG81A_002388"
     CDS             complement(2361..2723)
                     /locus_tag="ACG81A_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015710.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2727..3035)
                     /locus_tag="ACG81A_002389"
     CDS             complement(2727..3035)
                     /locus_tag="ACG81A_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015711.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3044..3463)
                     /locus_tag="ACG81A_002390"
     CDS             complement(3044..3463)
                     /locus_tag="ACG81A_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015712.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3456..3689)
                     /locus_tag="ACG81A_002391"
     CDS             complement(3456..3689)
                     /locus_tag="ACG81A_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015713.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3686..>4326)
                     /locus_tag="ACG81A_002392"
     CDS             complement(3686..>4326)
                     /locus_tag="ACG81A_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFS5015714.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1224 a          962 c          620 g         1520 t
ORIGIN      
        1 caatattctc ttcatgttgg tctaacaact gccaaccatt attactaaca gatcttaaat
       61 aaccattacc ttcatttagg cgcataagct gttcaaaatt taaaatcaac acatcatttg
      121 gagtgtgata ataaatattt tctttcagtt ttccgactat ggtaattcgg tcaattgacc
      181 actgcatttt taagggaagg gtcaaacttt gatactttaa ccgcctgtta gagggggctg
      241 ttaatttttc ccactcattt atgatcgtgc cttttttact caaattttgt tcctcctcaa
      301 agaaaaagag acttctggaa agaattttca aaagttcacg taactcttaa aaattcatta
      361 ccaaaaaatc tctagtcttg aagaaatcta aaaatattcg tataatgatt aagaagttac
      421 ggagctagtg cgattagttt ccgtaaaggt cgagaacgtg tccagcgttg ctcgacttta
      481 tttttttgct cttttttcag tttatttatt tgataactca ataatagcac ttctctttag
      541 ctatgcaaat atccaaaaaa taaaaccgca ccctttgaaa agggcgcccc caaacttcgc
      601 taacgctagg ttggccaaaa aatggacttg ccatttttta gcaaatgtca gcttcaggga
      661 ttagtggttt agccgtcaac tccttatcct cggcacaaac cgcctccggt accgttgact
      721 gcacatcgta actaccactc gatctcatct tcagaacgct ctaatttacg ctgtgagacg
      781 ttttgacgat aaaataaggt tttctcctgc tctaacactt ctaattcctc agaaagcccc
      841 atatcctttt ctgcaagacc aaaacggata gacaaacgag tgatcgattt ttgaaatgcg
      901 ttttttaatc gctctaaacg ctggcgtaaa gagcgatttt cttctttcag ctttttattt
      961 tccacttgta aatctgctaa ttgttttcta ccagacaaat gacgctcttc ttccaatgta
     1021 gctacatact catttaattc tgtattttgc tcaagcatct gtaaatattg ctcactcatg
     1081 ttatcagcat ttctcttggc atttgttgct ctcgtagctc gttgatacaa atcaacaaaa
     1141 tcattcccag ataaatgata cgaaccatca ggaagcctct tgaaactttc taaaggaaca
     1201 atatcttttt ggaaaatgac cgccatctct ttttttacat tcagcatatt tttcttctct
     1261 tcttcaatct tcactctctc agaagccacc agagcctttt ctcgctcatt ttcttctctt
     1321 tgcctagaaa aatatacctc aactcatcag aaacgctctc acgctctttt acggccgttt
     1381 gacgcaattc tgaaatctcg ctcatgattt ctttatattc gtgaacatct ttgattttgt
     1441 tagtaccgac taactcacgc tcccaaccaa aatctgccat caaacgttca attgactcaa
     1501 cttcctgatt cctaaaatta ttccaaatca ctcgaccatt ctctttatct gctcgtaaac
     1561 cctgctgttg caaggctttg ttaaaacttg gttgcttttg aacaccccgt ttataccctt
     1621 cagcaacagg aacaatattc aaatgcaggt gcggacttac ttcgtcatta tgaataacag
     1681 cattgtagac ggataaatta ggattccgtt ctttaaattc tttgaaatat tcaactaaaa
     1741 ttttgttggc aatcactcga ttttgttctg cttgttgttc cgatatgcca gtagaagaac
     1801 catcatcacg agtagtacga aaatcatcaa gagtaccaat ttgcacgata aattctcgct
     1861 gtggttccaa ttttttatcc ttgcgaacct ttgctaaata gtcctcgatt tttctatcgg
     1921 ctcttttctg ctttgcatta tatttttcta cggcttcacc gaataattct tcatattttt
     1981 cacgaatatt tgtttgttct aaatagatat tttcatccga ttttgtgaaa tcaatatgct
     2041 tatgccagtc attgattttt tggctatcag ttaactcacg attattatga gaaatgcttg
     2101 tattctttgt tccctttttg aacgaaatac tgaaccccaa gttaaaaccc tccttcggct
     2161 gtcgctaata aaaagagtgt aaacctttct gttactcttt gtcaaagagt aacattttcc
     2221 taattgtgac atggctgtca caattccaaa tgctctccga gggtattcgc tgtcacaagt
     2281 gacaacgaaa aaaggagccg aaagcgtgtc acgctctcaa ctccttttac gctttatttt
     2341 gcttcacgca aaataatcgc ttatcgtaaa ttcacgtcat taaatattga cgtaaattta
     2401 ctctcactca tctgactttt cttattcggt atcactccga ataagccgtc agattcatcg
     2461 ggataatctc gttcgcttgg cgctgatttt tcatcagcaa gccactcatt atccactgat
     2521 ttttcgtgtt gctgatcgtc agaaatcgac gatagccacc gctcaaaatc agcaaaagtt
     2581 tcaaacccaa aacgcttccg aagagccttg cccattgcag cattttcttt ctcaatcaag
     2641 cgttttttct gttggcgaag tttcgcttgt tcttttttta atttttcttc ttgcgcttta
     2701 atatctgcta aagtcattcc cataaactac atctcctttt ctttggaatt aataacggaa
     2761 atttggttta actgcataac atgactcatt aatttcaacc aaaattcttt ttctgattca
     2821 tcagttgttt gcgctaaatg cttatgaaca aattcattag tcaaaactga agattttgct
     2881 aaatcagcat tgataaccga gttgagaaca tccataattc gttcaagcat ttcactataa
     2941 ttatcttcat ccggcaaatt caaataatca gctaaccgtt catacatcaa ttcaattcta
     3001 tcgactgttt cttctctcaa atcaatttta tccatgtcac acctcacttc cagaattgcc
     3061 accattttcg attttttaat tcttgatttt ctagtaacaa ctgatcgttt ttttgttgct
     3121 gaagcagggt caatttttgt tgatcacgaa tcatttctgt taattcagaa atatgtttgt
     3181 ccttctcagc taattgatca cgcaaaaact cattctcttt ttctaaagga aaatcactgc
     3241 ctgtgttatt gaccggtaat tgaccagtca attgatcact ttttgaccga taaattgacc
     3301 gaacaacacc aatcaactct ttatctagca tgataatgcc attttcttta tactgagcaa
     3361 ggtcaaaccc tgaattgacc agttgattgc gcactgtatt tttcgataca cccaattcgt
     3421 cagcaagatt ttttatggtc atcttttctg acaaatcagt cattagcttc tccactactt
     3481 tcagctaccg cataaatata tttaacggcg tgcttaggga taataatcgg agagtcaatt
     3541 ttattatttt tcaaaatcaa ttcatattgc gttacatcaa gtaacttacc ggacattttt
     3601 ttgctatcca aaaaaacaac cacaacgtaa tcacctgtta aatttaccaa ccaatttttc
     3661 ttaccaaaat acttttgttt ttctgtcatt tttcttcctc ctctgttacg tctagcccta
     3721 gaagccatgt gtctaattct ttttttaaat cgtctgccga ttccgcaaag gtttctcgca
     3781 aatgattcgt caattcatca ttttgacttt cttgcttcaa caaatccctg tattttttct
     3841 ttgtattctt agctagattt ccccactcag aatgatcata aagcaaacct ttcaccataa
     3901 tccgttcagt aactttagta tttggcggaa aataatgtgg actagcaaag ctatccaaac
     3961 tttcatgaac catctcatgc caatcagttg cttttcccct tcgtaactgc aattctagtc
     4021 gccaccatgt cgttatttcc ggcggaacaa ttttacgttt tctttcttgt tccagttttt
     4081 tattatacat ccgaatttgt cgttcactgg cacgactacc ccaatatgcc gtttctaact
     4141 taccagaacg cccataaatt ggtttaaaag acacaggatc aactacccga tattgtgtaa
     4201 taaactcatc aggtacatcg ataatgtcgc aagcaatatc cgcacgtgaa aagtgaggtt
     4261 cgataaacaa atcatgaata aaatttttca tggaactagc taagaattga ctaatcttat
     4321 tcggat
//
LOCUS       DAZULY010000211         4295 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000211 DAZULY010000000
VERSION     DAZULY010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.214"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(17..913)
                     /locus_tag="ACG81A_002393"
     CDS             complement(17..913)
                     /locus_tag="ACG81A_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5015715.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(906..1586)
                     /locus_tag="ACG81A_002394"
     CDS             complement(906..1586)
                     /locus_tag="ACG81A_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5015716.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1587..2369)
                     /locus_tag="ACG81A_002395"
     CDS             complement(1587..2369)
                     /locus_tag="ACG81A_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS5015717.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2362..3279)
                     /locus_tag="ACG81A_002396"
     CDS             complement(2362..3279)
                     /locus_tag="ACG81A_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS5015718.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3443..3697)
                     /locus_tag="ACG81A_002397"
     CDS             complement(3443..3697)
                     /locus_tag="ACG81A_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015719.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT         1258 a          942 c          753 g         1342 t
ORIGIN      
        1 cactaatctt gtataattaa accctccgca acttgacgga gatggagaag atgcccccgt
       61 ctaatctagc ggtcatctct cctcccattc cttccaccaa tttttttgac aggtaaagcc
      121 cgattccgct agattccgta tttgtgcggc tggtattttc ggtataaaaa cgctggaaga
      181 ctttttcaac agaggtttgc gttcctggtt tgataggatt ggccactgtt aatcgaatga
      241 atggatcctc ttttgaaaat gtcacctttg cttgtccatc tgcataacgc aataaattct
      301 gtatgatatt ttgcagcact cgcagcaaca tgagcggatc tgctaataca gttgtattta
      361 tcgagtgttc agcgaacgct acctcgatat ttttctcaga aaattgttca taaaaagaga
      421 aaaatacttc ttccacctct ttagaaaaag aaagcgtatg gagatccatt tctttttgct
      481 tcgtctctat tcgagcaaga tcataaaact gatccgtcat actgagtaat cttgcaacag
      541 attgtttgat gatcgttaaa tacttctccc gtttctccgt atcctctacc tgttccagta
      601 attgtacata accgttaata gcagtcaaag gagttctgag atcatgagaa aggccgacga
      661 tcgatagtct gatgttttct tccatttctt gaaatgcttg tcctttttgt tcattttcat
      721 ccaaccatgt attcagtgca tctactaggt ccattagatt cttttctcga aattcgactg
      781 aaagacgacc accatattct tgtcgttcag aaagagtggc gattttctta gttaatctct
      841 tgatttccct ttgcaggaag aaaataaaac ttcccataaa gataacaacg agaagcaaca
      901 gccatttaat caaatctaaa ccccactccc cacaccgtat gaatatacac agcatgtgcc
      961 tttgctagct tccctcgaag attggaaata tgcatattca ctgttttctc actatcaaaa
     1021 tatggttctt gccaaactgt ttcataaaga tttgctcgcg aaaatacttt tttcggatgt
     1081 tctaaaaaaa gcttcagcag atcgaattct cttcctgtca aatggacagc cacaccattc
     1141 acatttacat ccctcgactc tttgtccagt aagatttcct tgtaatagat gcgttgcgac
     1201 agatctttta cttggacaga agtctgatga cgaagttgga tgccgattcg taacaaaagt
     1261 tcttcgatat caaatggttt cgtcacatag tcatccgctc ctgctgccaa caaggaaact
     1321 ttatctcttt gatcgctttt tgctgaaaga acgatgatcg gaacattcga tgtctgtcgg
     1381 atttctgcta acaatgcttc acctgaaagc cctggaagca tgagatctag aaggatcaaa
     1441 tcgacaggat atgtgcttaa caataatttt ccttccgtac ctgaataagc atcaagaagt
     1501 ttgtatctct gttcaagaac ctcctccagt aaggaatgaa cagcttcatc gtcttcgata
     1561 atcaaaatag tttccagcag gaccacctac ttaatgtcag atttttggaa aataaacatt
     1621 cctagagctg atgataccac gattgttact agactgacca gaatcatttt cggtgcttgc
     1681 gaataagttg cttgttgcgc aaagtccgta tacatgagcc acgtttccgt aaaccactcc
     1741 gttagaaatt ccaagccttt gatttttgcg aaaacaccgg gaatgaaggc cgttcccatg
     1801 atataaagaa aagaaagaag caaactgaca ctggtacttc tagtgatgaa tgtcaaagcc
     1861 actggaacag ataatgtccc taatgtcagc agaagcaata caggaaatac ttttagaaca
     1921 taatttccat agtttttgat tccctcaact tcaccaaatc cgaaagcaag tgtgtaagca
     1981 agcatggcta gtcccgtgat caaaaccacg ccaagtgttg tgatcaaaag cgtaacgaga
     2041 agtttcgaca cataataagt cattcgattc cgtcctaaag ataacgtatt gcgaatcgta
     2101 ccgttttgaa attcttcccc aatataaaag cacgcaaatg ctgcgaaagc cagaagaatc
     2161 catgcagcca ttttttctac cattaaaatc cctgttgcac cgttgatgac tgtatccgtt
     2221 gcttggttta attcattgcc gtattttgta tgcatatacc attcgttcca agaataaaaa
     2281 agcggaagta cgaccatcag tcctagtgag acccacattt tacggctctt ccatactttt
     2341 attttttcag cagttaatag attaaacatg tggattgcct ccagttaatt gtaagaaata
     2401 atcttccagt ttttgcccaa ctaggtgaat accatccaca ggaacttttg cttctaccaa
     2461 tgcttgattg atggctgcta cttggtcaat tccttcaaaa agctggataa cacggctaga
     2521 ttgtacaaaa taatccctat aaccaagaga gtcgagaacc gttactgctt gttccgtcgc
     2581 cgaagtgtca atttttataa attgacgaga ttcttgtgcc aattcttctt tcgataattc
     2641 tttgatcaag tgtccttcat gcaaaatacc atagtgagtg gcgatctgtg acaattcatc
     2701 tagcaagtgg ctagaaagca agacggtgat gccccgttct tttaccaaac gttggatgat
     2761 ttctcgcatt tctgccatcc ctgctggatc catcccattc gtcggttcat ccaaaatcaa
     2821 aaattctgga ttcgtgatca gtgcatttgc aatagctaaa cgctggcgca tacctaaaga
     2881 gaaattcttt gctttctttt tccctgtatt ttccaatcgc atcattttca gcaggtcttc
     2941 gatttctcta tccgacacac cgccgttcgc tgcttggata cgtaggttat ctcttgcagt
     3001 taattctgga taaagagcag gggtttcaat cgattgaccc atacgtcgtc ttgctgcctg
     3061 caaatccgtc gttccaaata attcgatcga accttcatca atcgagatca aacccataat
     3121 agttctcata aaggtagact tacccgctcc attttcccca atcaatccat agatcattcc
     3181 acgtttgatt tcgattgata ctttatctaa tgctttggca gcaccgtatt ttttggttat
     3241 accagttgct ttcaagaccg tttcatttcc aaaatccata cttatccttc tttcttattg
     3301 atgagtgaag tatagttgac gggtataaag aaagcagcca gaaatgtata aagaaagtgt
     3361 aaattttcca attgaatgtc aaaaaaaaca atcggacaat acgtgtcttt cctattatca
     3421 ggcagtttgt tatacgcttg tatcagtttc ctgattcttt cttttcgacc aaagtcgttt
     3481 ctctgtattc ctcttcactg aaaatttttg cttctttcag atcaagcttg agtaatttta
     3541 ccagaccggt tataaggata actatagcaa gactccctcc cagaaaaatt agactgtctc
     3601 gcagacgtgg ttgcgttgtc ttccaatcaa agagcgcacc aaacagtgct aatccaagtg
     3661 gactggccac catggtaatc gttgttatcg ttgacataag tcgtccttgt atatgcgttg
     3721 gaacttctcg ctgaagccag accatataaa aagcaaaaga aaacattaat ccttaaaaac
     3781 ggacttatga aaaataatac atgccgtttg ggatttacaa ttagctacca aataagaggt
     3841 tatgacaaaa gacttttcac aatcccttct tccgaataaa cggtgttctc ctgccaactc
     3901 ctcggtcaca agtcacaata tttatcaaac atgagaagca atttttgtaa attccttctt
     3961 atttcttgaa gctgaccact tctgtcacaa cctcagaagc ggataaataa tattgtttga
     4021 tgatacttta tctttttccc gtttgtttat agatcgaatg atattataga ccaagaatcc
     4081 tacctattaa catagctaaa aacacaaaaa aggcactttc caaattgtgg aaagtgccag
     4141 aaaagttagg attaaggtaa ctgcgctcag cattgcttcg cccagtacga ttctcgtaac
     4201 tcgtgaactc gaacgataat cgcaattaac gttttgagaa ctgtgaagct ttacgggctt
     4261 tcttaagacc tggttttttc ttcgtatcat tgaat
//
LOCUS       DAZULY010000212         4279 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000212 DAZULY010000000
VERSION     DAZULY010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.215"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACG81A_002398"
     CDS             complement(<1..149)
                     /locus_tag="ACG81A_002398"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5015720.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACG81A_002399"
     CDS             complement(593..1714)
                     /locus_tag="ACG81A_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFS5015721.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACG81A_002400"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACG81A_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFS5015722.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACG81A_002401"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACG81A_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFS5015723.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
BASE COUNT         1413 a          774 c          809 g         1283 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttg
//
LOCUS       DAZULY010000213         4276 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000213 DAZULY010000000
VERSION     DAZULY010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.216"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..1348
                     /locus_tag="ACG81A_002402"
     CDS             47..1348
                     /locus_tag="ACG81A_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS5015724.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1375..1635
                     /locus_tag="ACG81A_002403"
     CDS             1375..1635
                     /locus_tag="ACG81A_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS5015725.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2634..3869)
                     /locus_tag="ACG81A_002404"
     CDS             complement(2634..3869)
                     /locus_tag="ACG81A_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS5015726.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1242 a          684 c          882 g         1468 t
ORIGIN      
        1 gtcatgcaat gaatggttca tattttattg aaaaggagtg gttttagtgg aaaagctgat
       61 gaaatgggtc gaagagaaac ttgtcccacc aatggcaaaa attggtacac agcgtcattt
      121 attagcaatt cgtaatgggg ttgtttcaac attgtcattg attttgattg ggacattttt
      181 tatggtcttt atcaatttac cgtttcctgg atggaatgaa tttattgccc cttattcggc
      241 aacgattgtt ttacccttta ggatgacgat gggattgatg gctatttatg caacttttgt
      301 tatgggttcg gatctagcta aatcatatgg attagatagt gtgacaggtg gtatattatc
      361 tttaggaacg ttctttatgt tacaagttcc tgtaaacgtt ttaactcctg aagaggcacc
      421 tcttggctgg gtcttaccta tgagttcttt gggagcatct gggatgtttg cggggatctt
      481 atctatgatt tttgcagtgg aagtatatcg tttctttaaa aatcgaaata ttactatcaa
      541 aatgccagaa caagtgccac ctgctgttgc ccgtagtttt gaggcattga ttccaggtgc
      601 agtcatttta acaattactt ggctgattcg aagcgtcgcg ggctttgatg taaacgctgc
      661 cttattatcg ttattcgagc ctttaaccaa tattttaggg aataatttgc ttggtgtttt
      721 actaccaatg tttttgatcc atttgttgtg gtcatttggt atccatggca tgagtattgt
      781 aggggctgtt gttcgtccaa tgtggctgat catgttggat gagaatgcaa aggcattggc
      841 agatggtaca gctgccaccc aattacccta tatcgctcct gaacagtttt atcaatggac
      901 ggtaacaatt ggtggtgcag gagcaacgat cgtggtatct gttttgttta tattcttctg
      961 taaatcaaaa ttcttgaaag aggttggtcg tttttctatt attcctggta tcttcaatat
     1021 caatgagccc ataatttttg gagctcctat gatgttgaat ccttacatgt tcatcccgtt
     1081 caacttagtt cctttagttt tgactattat cagttatttt gcagtcaaat tagaaatggt
     1141 caatagattt actgttttgc caacatggac attaccagct ccaatcggcg gttatctttc
     1201 agcaggaaat gactggcgtg ttgttatctt gatcatgatc aacacattga tagcatttat
     1261 tatctattat ccatttgtaa aagcttatga taaaaagatg ctgtcagatg aacaaagtcg
     1321 tggagaaaac gaagtccaga ctgtctaagt ttggttttta gaaggtggga aacaatgtat
     1381 tggctgattt tattttttgt ttttatcttt ttattaacgg ctagtcacct tatattgaat
     1441 atgctagcta catatcatat tcaaatcaat cgatggattt gggcactggc atcatttttg
     1501 atcgtgattc ttccaaagat cattgtaccg catatgaatg ttttgttttc atgggggaca
     1561 tatgtacttt gtggaatttt tgctatcaat tttatgatcg agcagcatag atggtttgtc
     1621 acatcgaagc tttaaatgtt ctaaaagtgg ttgtaacaga agtggttagc ttcaagaaat
     1681 aagaagaaat ttacgaaaat tgcttcttaa atttttgtga aatgttggct tatttttcgt
     1741 ttggttgaac ttattaccgc agatgcaagc ttttgaccac cgtttattcg gaatatggga
     1801 ctgtgacaag gcttttgtca cagccctttt ttacataaag attattattg ttttaactgt
     1861 tttggcagaa agaaggagga aaaaatggaa attttatcag caatggattt agaattagta
     1921 gatctactac caaagtgatc aagtcaaata tagatttggg agcttacaac catatagtag
     1981 gggctgcagt atggtgtatg aatggtaata tgtatgatgt gagtgtttat tcaaatcatg
     2041 aaacgtgtat agaaataatt gtgctgggaa tagcaatcag tcatggtgaa cgtgactttt
     2101 agtgtatcgt tgctgacgac atagaacatt ctgacatgat ctactcaata tgtggggatt
     2161 gctggcagtt tatataggag tatgcatcgg attgcgagat cattgtctca acagaagagg
     2221 gaggttgatt atcaaagata aagtggtaaa tcctacattt gatttagtaa ttgttgaggg
     2281 gactagcggc acaaatctct acaaagcgat gcttctagat ctatatgaca cattttaaga
     2341 aaatgtattt gcctaattat tatggtcttt cttttgaaga aacgttgaga tgatccaacc
     2401 agagaaaggt gtcaaagaat ttgactaaga ataaatggca gacgctacct atgctcattt
     2461 ggatagcgtc agatctttta ttttaaggat gattttgtgt tttttagaca ccgtaattta
     2521 agtaactcta acttttcaat ccactattta ctttttttgt attttcactg tctgggtctc
     2581 gaatagcgaa attactgatg agtctctatt aaaaaagctt tttctacttg aaactatatt
     2641 gggtggttgc tcaggaaggt ttcgatttct tgaattgctt gttgtggacc atttattgga
     2701 aaaacggctt gcagcgtgcg tgcctgctga cgaatcttgg gtgaagaaac ttcgcgcaga
     2761 gcaatctgca gttttctttc agtcactggt tttgtgaggg cgattgcgtt tccgtatttt
     2821 acacaatagt caatattaca gctttgctca aattgcatcc ccacaccgat aaacggtttg
     2881 ccggattcac aggcagtttg gacggtccct tccccgccgt ggataaaaga aaaatcaatg
     2941 aattcatgca aaagatgact agacagatat tctgttatta aaagatcttc tgagtcagat
     3001 tgaatgtcat caagcagata gtcaataggt gcaataaccg ctagatccgg ttgtttttgt
     3061 aagaagaaca aaattttgcg taacatacgc ggattgcctg aactacccat tgcgaaatag
     3121 ataatacgtt ttctttcttg ttgctttcgc tgtataaaag ttagcacttc ttcaggcaag
     3181 gaagtactga gcttagcaaa aattggacca acgattcgat aattttccgg cagctgttct
     3241 tcttccaaaa atagtttagg cgttgtgatg agatttagat cagcatccat catttgtaaa
     3301 cttgtttttg gtaaaggaag catttctttt tgtgcagcgt gtttgaaggc attggggatc
     3361 catcgggtat ttactatacg cttttttgtc cagtgccaaa ggggattgct taaagggccg
     3421 aagaaacgta actcgtgggg catcagcgca tgctctgatt gaaaaaaagg ttcactcaaa
     3481 gcatagggtt taacatatac taaaggaatc tttttattgc gggccgataa aaaaattgtc
     3541 acattcgaac ctgtcacaat cagtcgaggc tcaaacttag caatcagact ctgttcacta
     3601 gtgatacgag aggcaaccat ttctttggta aaagggtttt taagtgtacg caactggtca
     3661 aattttatga tttgttcttc ttgttttttc gtaaggatcg gcgctagtaa atgatactca
     3721 aagccacttt ctgtgatcaa aggagcaaaa gttttagaaa agccaaagaa tacacagtaa
     3781 taacggtctt tcatagcttt ggcaacctca atcattcgtg ttgtctcagc taaattgaat
     3841 actgcaggag caaaaagaat tgtcttcatg aaatcctcct tattggaatg agtctatccc
     3901 ttcgtttctt actagtatac taaaaaacac agcgttcgag tagcctatgt atattttgaa
     3961 tattaaactg tctctcgctg ttttcacaac ttctaaattt aaattttgta atcaagttga
     4021 aactccaatt ttcaaaaaaa cacgcaacaa ttttttgatc gatttatatg ttgtgccaga
     4081 taaatacgag aagttgtgtc attaaaaata aacatgagaa cataaaaaaa taaaataatt
     4141 atattaattg ataggtgtgc tgtcgtcatt atacacacgt aagaaagaaa tagttatgtt
     4201 taaaaatatt cttagacttt ctcgccatga caattttcac tatgtgttat aactgtaaac
     4261 gataattatt cagata
//
LOCUS       DAZULY010000214         4267 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000214 DAZULY010000000
VERSION     DAZULY010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.217"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            62..337
                     /gene="rpsP"
                     /locus_tag="ACG81A_002405"
     CDS             62..337
                     /gene="rpsP"
                     /locus_tag="ACG81A_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFS5015727.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            349..600
                     /locus_tag="ACG81A_002406"
     CDS             349..600
                     /locus_tag="ACG81A_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFS5015728.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            725..1516
                     /locus_tag="ACG81A_002407"
     CDS             725..1516
                     /locus_tag="ACG81A_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015729.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            1686..2207
                     /gene="rimM"
                     /locus_tag="ACG81A_002408"
     CDS             1686..2207
                     /gene="rimM"
                     /locus_tag="ACG81A_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFS5015730.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            2197..2958
                     /gene="trmD"
                     /locus_tag="ACG81A_002409"
     CDS             2197..2958
                     /gene="trmD"
                     /locus_tag="ACG81A_002409"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFS5015731.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            3094..3441
                     /gene="rplS"
                     /locus_tag="ACG81A_002410"
     CDS             3094..3441
                     /gene="rplS"
                     /locus_tag="ACG81A_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFS5015732.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(3573..>4267)
                     /locus_tag="ACG81A_002411"
     CDS             complement(3573..>4267)
                     /locus_tag="ACG81A_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5015733.1"
                     /translation="SYAMKKGYVEKNPINEVVIDYKRESKTIKIKDKFLEDDEYKRLV
                     NFTTSHNKRYSLLFQWLYLTGMRPGEAIALSKDDVHITNNNASVVINGTMMYRERSIA
                     DMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNPNGQFLFQTTKGTPFQLTAINTYL
                     RNHKADMKIDKKLSSHIFRHTHISKLAELGTPLYAIQDRVGHENSDITEKIYLHVTKG
                     VKEKLKEDIEKL"
BASE COUNT         1411 a          760 c          848 g         1248 t
ORIGIN      
        1 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
       61 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
      121 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
      181 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
      241 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
      301 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
      361 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
      421 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
      481 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
      541 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
      601 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
      661 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
      721 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
      781 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
      841 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
      901 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
      961 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
     1021 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
     1081 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
     1141 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
     1201 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
     1261 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
     1321 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
     1381 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
     1441 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
     1501 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
     1561 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
     1621 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
     1681 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
     1741 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
     1801 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
     1861 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
     1921 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
     1981 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
     2041 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
     2101 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
     2161 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
     2221 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
     2281 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
     2341 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
     2401 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
     2461 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
     2521 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
     2581 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
     2641 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
     2701 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
     2761 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
     2821 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
     2881 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
     2941 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
     3001 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
     3061 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
     3121 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
     3181 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
     3241 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
     3301 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
     3361 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
     3421 atcaaagaaa tccgtcgtta atcatcgtta tcagactgag aaagaaccct tggtattcaa
     3481 gggttctttt tttgttctct gtttttttat attaacgatt tctatttggg acatttttga
     3541 ttaataaaaa ggggcaaaaa aggggcaaca agttacagtt tttctatatc ttctttcaat
     3601 ttttctttta ctccctttgt cacatgcaaa taaatctttt cagtgatatc actattttcg
     3661 tgcccaacgc gatcctgaat agcatacaga ggagttccta gttccgctaa ttttgaaata
     3721 tgggtatgtc taaaaatatg tgaactaagt tttttatcaa tcttcatgtc agctttatga
     3781 tttctcaaat aggtgttaat tgctgttagt tggaaaggag ttcctttcgt tgtttgaaat
     3841 agaaattgac cattcggatt taattctaga agctcattgt aaatagctat cgcttttttt
     3901 ggtaaatcaa tttctcgcat tccagcagca gtttttgtag aatcagattt tttcatatca
     3961 gctattgaac gttctctata catcatcgtc ccatttataa ctactgatgc attattattg
     4021 gtaatgtgta catcgtcttt acttaatgcg attgcttctc caggcctcat tccagtcaaa
     4081 tatagccact ggaaaaggag agaatatctt ttattgtgtg aagttgtgaa atttactagt
     4141 cttttatatt catcatcttc taaaaactta tctttaattt ttatcgtttt tgactctcgt
     4201 ttataatcaa taactacttc gttgatagga tttttttcta catagccttt tttcatagcg
     4261 tatgaaa
//
LOCUS       DAZULY010000215         4209 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000215 DAZULY010000000
VERSION     DAZULY010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.218"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            362..1090
                     /locus_tag="ACG81A_002412"
     CDS             362..1090
                     /locus_tag="ACG81A_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5015734.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            1108..2304
                     /locus_tag="ACG81A_002413"
     CDS             1108..2304
                     /locus_tag="ACG81A_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5015735.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            2297..3292
                     /gene="lacD"
                     /locus_tag="ACG81A_002414"
     CDS             2297..3292
                     /gene="lacD"
                     /locus_tag="ACG81A_002414"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFS5015736.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            3289..>4209
                     /locus_tag="ACG81A_002415"
     CDS             3289..>4209
                     /locus_tag="ACG81A_002415"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HFS5015737.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHI"
BASE COUNT         1412 a          675 c          919 g         1203 t
ORIGIN      
        1 agtataaaac tgcaaaagtt atctaaaact aataataaca tttgtcatta ataagctatt
       61 tttagttgtt gtaaatgata ttggaagtaa taaaacgata gttgtaggaa tatcataggg
      121 attaatgtgg atcacgctgc tcgataagtt agaccatttt gacggatcgc ttatgtggtt
      181 cgtctttttt tctgttcttt tattggtaac tggtattttt gaggtatctt tgtctattca
      241 agatataaag ctttaaagag ctgttataat agcttttttg gtctaaaaaa cctttgacaa
      301 atgattaata attcactatt cttgtgttgt ggttataacc agtaaagaag aggtgatttt
      361 tatggagagc tcaaataaag gaaaatcatt gtattatcaa ctagttgatt tattgaagga
      421 tcgtatcgaa acacagatga ctccgcatga taagctgtta tctgaaagag aactttgtca
      481 acactatggg gtgagtcgta ccaccgtacg tctggcttta caggaattgg aaaatcaagg
      541 ttatatttac cgaagacatg gaaaaggtac ctatgtctct gatttgtctg ataagtcatc
      601 gaacttagct ggagcatata gctttacaga agaaatgcgc gaaatagggc gcgtccctaa
      661 gacggtcatt ttagaattta gacaaatgga agctaataag agaattgcta agaatttgaa
      721 catccattta ggtgaaactg tatttaaact taaaagattg cgtttggcag atgaagagcc
      781 attgatggtt gagcgttctt atctaccggc gaaactattt atgggacttg atttgccgtt
      841 acttgaaagt aaaccgttgt atgatgtgtt ttcacaggat tatgaacaac aagttcaatt
      901 agctgatgaa gagttttatg caagtatcgt gcaaaaaaaa gatgctgttg tgctacgaat
      961 cgctgatggc gctcccgtct tacatctgac gaggataacc tataacgtga aaaatgagat
     1021 tatcgaatat acagatagtg tagcaagagc ggaccagttt cattataaaa ttcggcatat
     1081 ccgaaaatga ttgagaggga gaacaacatg tttgagctat cgaagaacaa attaaaacaa
     1141 ataggtgcag caatcacgac acaagaaata aaacaacaac cagatctctg gaaagaaaca
     1201 atcgatagat tgatgcaaag ccaagaaaat ttagctctat tttttgatca aataaaaaaa
     1261 gaagcaaacg gaagaagaat acgtgttatt tttacgggtg caggaacgtc tcaatatatt
     1321 ggggatacgc tcttacccta tctaaatgaa catggagaca atcagttttt tatcttccac
     1381 agttttggca cgacagatat tgtagcttca ccaaaagaat ttttgtttcc aaatgaaccg
     1441 actttattag tttcatttgc tcgcagtggc aacagtccag aaagtgtagc ggctgtgcaa
     1501 attgcaaatg aacaagttga aacattgttt catttgacga ttacttgtgc taaagagggg
     1561 atgttggcaa aaaacgcaca ggatgataaa cagaattatc tgttattgat gccagagaga
     1621 gcaaacgatg caggttttgc tatgacagga agttttacgt gtatgttatt gagtgcttta
     1681 tggatctttg atcaaacagc aatggagaaa aaagaaagat atcttgaaca attagttaaa
     1741 atcggtcaag atcttctttc aagagatcaa gaaattcaaa agttattacc tgattttttt
     1801 gaacggatcg cttatctggg ttcaggaagt ttggcaggtg cgacaagaga agcacaatta
     1861 aaaattttag aattaacagc aggtaaagtt gctacaattt ttgattcttc gatggggttt
     1921 cgtcatggac caaagtcttt tttaaatagc gagacactcg ttgtaggttt tgtgagcaat
     1981 caaccatata cgcgaaatta tgacttagat attttagagg aaataaaaga agatcgggtc
     2041 actgccaatg tcattgcgat tgcgcaaccg ggtactattg atttctcagg tatgactgtg
     2101 ctattggaag actcggtgag taaattgccc gatgcctatc ttgcattagc gatgattatg
     2161 actgcacaaa tcattgcact ctatacttca atcaaagtag ctaataaacc agatacacca
     2221 tctccaacag gcacggtcaa tcgagtagtc aagggcgtca ttattcataa gtatgaaacc
     2281 gaaggagaaa tgacagatga atgaagaaaa aaatagggca atggagagac tatcaacaaa
     2341 agatgggatt attagtgcat tagcgatcga tcagcgtggt gctctaaaaa aaatgatcaa
     2401 atcgttggga aaagagccaa gtgaagacca aatcagtgaa ttcaaagaac tagtatcagc
     2461 tgagctgaca ccctatgctt ctgctatttt attggatccg gaatatggat tgccagcagc
     2521 aaaagtcaga gcaaaagaga cggggttatt gttagcttat gagaaaaccg gctatgacgc
     2581 aactacgcct ggtcgtctac ctgatttatt agcggattgg tcttctctca gattgaaaga
     2641 acagcaagcg gatgcagtaa aattcttact ttactacgat gttgatgaag cacccgaaat
     2701 caatcatttg aagcatgtgt tcatcgaacg tttaggtagt gagtgcgaag gagaaaatcg
     2761 tcccttctat ttggagttag tttcttatga tgcacaaata agcgatagtt cttctccaga
     2821 gtatgcgaaa gtcaagcccc acaaagttat tgagatgatg aaagaatttt ctgatccaaa
     2881 atataaagtt gatgtgttga aagtagaagt accagtcaat atgaattatg tgaatggcta
     2941 ttccacaggt gaatttattc acagcaaaga agaggctgct gccttcttta aggagcaaag
     3001 tgaagcgact ccattaccat tcattttttt gagtgggggt gtttcaacag aattatttca
     3061 acaaacactg atttttgctc atgaatcggg ttccaccttc aatggtgtat tgtgtggacg
     3121 tgcgacatgg aaagatgggg tcaaagcata cgtcgaaaat ggaaaagacg gagctattca
     3181 ttggttaaga acaaatggga gagaaaacat cgagacttta aataaagtaa taaatgaaac
     3241 cgctacccct tggttcaata aaacaaaaag caaaaaggtg aagatgaaat gattacgaca
     3301 gttaccttga atccttccat cgatatcgcg tatacaattg aagaattaaa cctcgacaaa
     3361 gtcaatcgag ttgaaacagt caataaaaca gcgggaggaa aaggagtgaa tgtggcgagg
     3421 gttctaaatt tattaggaga agaggtgata gctactggtt taataggtgg ccagcacggt
     3481 gaatatctaa agaaccagct agatcagtta gggatcatgc atcagtttgt tccaattaaa
     3541 ggagagacaa gggattctat tgctatcctg catagttcga gacaaacaga gcttttggaa
     3601 cgaggcccct tcataacaaa gaaagaacaa cagacattta ccgatcattt cgagcagctc
     3661 ctcgaaatat cagatattat gacgatctca ggtagtttac cccaaggatg ccctcttgat
     3721 tactataagc aattggttgc tttagctgat aaaaaaggga aagacatatt actagatact
     3781 tctggtaaat ttttgcttga agtattggaa ggtaaggaga agccactact tatcaagccc
     3841 aatctggaag aattaggaga actgattggc caacgtatct gttcttcgat tcaattagaa
     3901 aaaattcagt ccttattagc tcatccaata tttgaaggag tgccatgggt tgttgtttct
     3961 ttaggagaga gaggtgctgt ggcaaaagtt gctcagtgtt tttacgaagt cattattcct
     4021 aaaatatcag ttgttaatcc tgttggttca ggtgacgcga cccttgcagg atcggcccat
     4081 gcgttatcaa aaaggcaatc aatcgaagag attctcgcat tttctatggc ttgtggtatg
     4141 gcaaacgcgc aacaagagaa aactggctgg atcgacccta tgaaagttac gatgttgatg
     4201 aaacacatt
//
LOCUS       DAZULY010000216         4190 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000216 DAZULY010000000
VERSION     DAZULY010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.219"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..537
                     /locus_tag="ACG81A_002416"
     CDS             <1..537
                     /locus_tag="ACG81A_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HFS5015738.1"
                     /translation="DGYNSDLISEYKNEIETCVNEIRKLNWDIVSVKKEIQDKAKVEL
                     EIEDLKKQVEKLSHKSYKDVYDEYSKETDIYRELKRDVEALKEIPKNLTDSVDKIDFG
                     NGIKVTDEIDEHRGELIKNLVTNRAKIQTIINEMSDEIDSYRAELNKSDWKVNYNEVS
                     KRYQRGIKNLVKSYLKMN"
     gene            540..1436
                     /locus_tag="ACG81A_002417"
     CDS             540..1436
                     /locus_tag="ACG81A_002417"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS5015739.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            1486..1875
                     /locus_tag="ACG81A_002418"
     CDS             1486..1875
                     /locus_tag="ACG81A_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5015740.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            1862..>4190
                     /locus_tag="ACG81A_002419"
     CDS             1862..>4190
                     /locus_tag="ACG81A_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5015741.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         1434 a          648 c          882 g         1226 t
ORIGIN      
        1 gatggatata actctgattt gatttcagag tataaaaatg agattgaaac atgtgtaaac
       61 gaaataagaa aattaaattg ggatattgtt tctgtgaaaa aagaaataca agataaggcg
      121 aaggtagagc ttgaaattga agatttaaaa aaacaagtag aaaagctatc tcataagagt
      181 tataaagatg tttatgatga gtattcaaaa gagacagata tttataggga actgaaacgt
      241 gatgtggagg cacttaagga aattcccaaa aatctaactg atagtgttga caaaattgac
      301 tttggcaatg gtataaaagt tacagacgaa attgatgaac accgcggaga attgattaag
      361 aatttagtga ccaatcgggc taagattcaa acaataatta atgaaatgtc agatgaaatt
      421 gatagttata gagcagagct gaataaatca gattggaaag ttaactataa tgaggtttca
      481 aagaggtatc aaagaggtat caaaaactta gtgaagagtt atctcaagat gaattaaaaa
      541 tgttacaaaa ccttgattct ttatatcaat ctttatcaaa aactgaagag cgattggaag
      601 aaattattca acaagaaata aagcttaaag attaccaagt tgaggttgac tcagcaatgt
      661 caagaatgaa taaatcatat caaaatctct attctgagag aagtagttcc tcgcgcgaaa
      721 ttttttctga tattacagga atagaggcta ctattcaacc aaatagggat ttcaataatt
      781 atgttaaaga gctcagagct tgtattaata aggaggacac ttttgacagt gagtttggat
      841 ctcttattga taagctgaca aaaaattcca ttactaatca agatatatat aatgacatac
      901 ttgatataag agttagtggg gaaagtagta ttttttcaga taaaaaatta atcaattcta
      961 taagaaaatt gaatgatgca caagttcttg aattacaaac ccttaagcca aatgatttag
     1021 tagttatccg acttgaacta aatggaaaaa aagttagttt atcgaatgct agtgctggtc
     1081 aaaagacatc tgcaattctt tctatgattt tggcgtatgg aaattcacca ctcatattgg
     1141 atcagcctga agatgatttg gatagtcaat taattaataa cttaatcgta agaagtatca
     1201 ttacaaaaaa ggaaaacaga caaattttaa ttgtaactca taatgcaaat atcccagtta
     1261 atggtgattc agaatggttg attagcatgg gagattcaaa agaaatatcg gctgatcagt
     1321 gcgggtcggt agatgaaccc aatatcaagc caagaatatg tactgtgatg gaaggtggcg
     1381 aggatgcttt tacaaataga gcgaaacgct atggttttaa acaaattgta gaataacact
     1441 agagggtgag acatattatt atgtccgccc tttttaggag gaaaaatgaa aaaaataaaa
     1501 agctatacca gtatctgggc agttgaaaaa gtcatttacg caatcaatga ttttcagttg
     1561 ccttttcctg tgacttttaa ccaaatggct tggttcgttt tatctttact gtttgtcatc
     1621 gtatttgccc atgtgcctcc actatctatg atagaggagg tctttttaaa atacttaggc
     1681 ataccggtgg ctgtcacttg gtttatgtcg caaaagacgt ttgatggcaa gaaaccactg
     1741 ggatttttac gatcctttat cagctatcac ttgcgattta aggtgacttt tgcaggaaag
     1801 aaagtcaaag aacagaagaa acgatgggat gaacccagta ctcttgtaag gagtgtgaac
     1861 tatgtatccg attaagtata ttgaaaataa tctcgtcttt aaccaagaag gcgagtgttt
     1921 tgcctactat gaattagtgc cttacaatta ctcctttttg tcacccgaac agaaatttca
     1981 agtgcatgat aactttcgcc aactaattgc gcaaaatcgt gaaggcaaga tccatgcgtt
     2041 acaaattgcc acagaaagta gtattcgcgc cacacaagaa cgatcaaaaa aagaaattac
     2101 cggtagactc aaagaagtag cgaaacaacg aattgatata cagacagatg cattagtatc
     2161 catgattggc gatagtcaga ttgattatcg cttttttatt ggctttaaac taattgcgac
     2221 agatgaagaa gtcaatctaa agagtctcaa gaaatctttc ttttctgggc ttcaagaatt
     2281 tgtctatgga gtgaatcatc atttgatggg cgattttgtt tccttatcca atgaagaaat
     2341 tcgctgctat tctaagcttg aaaaattaat ggaaagcaaa ttggctcgaa gatttaaagt
     2401 caggcgagtc acaccgagtg acctgacgta tttgattgaa catatttatg gcgaaaaagg
     2461 gataccgttt gaagaatacg aatttcagct accaaagaaa aagctaaaat cagaaacact
     2521 ggtgaaacgt tatgacttat tacgtccaaa tcgttgttta attgaagaaa aaccaaggtg
     2581 cttacggatg gaacatgaga atcatgagtc gtacgtagcg tatttaacaa ttaatacaat
     2641 tgtgggggag atggaatttc cttcctcaga acttttctat taccagcaac aacaatttac
     2701 attcccaatc gacacaagta tgaatgtaga gattgtgacg aataaaaagg ctttagctac
     2761 cgttcgtaat aagaaaaaag aactgaaaga tttagacaat catgcttacc aatctgacaa
     2821 tgaaacgaat tccaatgtct tagatgcttt ggattccgtg gatgaattgg aaaccacctt
     2881 ggatcagtca aaagaatcta tgtacaaact gagttatgtg gttcgagtga gtgccgaatc
     2941 agtggatgaa ctaaagcgac gctgtgatga agtgctcgat ttttatgatg ataccaatgt
     3001 aaaactagtc cggccctttg gcgatatgat ggggctacat gaagaattct taccctcaag
     3061 taaacgatat atgaacgact atattcagta tgttacgtct gattttttgg cggggcttgg
     3121 ttttggggct actcagatgc taggagaatt agaagggatt tactttggct acaatgtgga
     3181 tacaggtcgt aatgtttacc tgaaaccggc attggcttca caaggagtca agggttcagt
     3241 aaccaatgcg ctggccgccg ctttcctggg ttctcttggt ggaggtaaat cctttagtaa
     3301 caatctctta gtctactatg ctgttttgtt cggtggtcaa gctgtgattg tcgatccaaa
     3361 aggggagcga ggagggtgga aagaaacctt gccagaaatt gcggatgaaa tcagtatcct
     3421 caacttgacg agtaagtcag aaaatcaagg tctgcttgat ccttatgtga ttatgaagaa
     3481 aaagaaagac tcggaaagtt tagcaattga tattctgact tttttaacag gaatttcaag
     3541 tcgagacggg gagaagtttc ctgtgttacg taaagccatt cgaacggtga ctcaaagtga
     3601 tcaacggggg ttattacaag ttattaacga attgcgaaaa gacgggtcac caatcgcgga
     3661 aaatatcgcc gatcatatcg agtcctttac cgattatgat tttgctcatt tgcttttctc
     3721 agacggctcg attacccaat cgattagttt agataaacag ctaaatatca ttcaagtggc
     3781 ggatttggtt ttaccggatg cagaaacgac ttttgaagaa tataccacca tggaattgct
     3841 tagtgtttcg atgctaatcg tcatttccac ttttgccttg gactttatcc atagtaaccg
     3901 agaaatcttc aaaattgtcg atttggatga ggcttggagt tttcttcaag tggctcaagg
     3961 aaaaacgtta tcgaataagc ttgttcgtgc tggtcgggcc atgaatgctg gggtctattt
     4021 cgtcactcaa aatagcgacg atttattaga tgaacgttta aaaaataata tcgggttgaa
     4081 gtttgctttt cgcagtaccg atattcatga aatcaagaaa accttggaat tctttggttt
     4141 ggatcaggag gatcaaagca atcaaaaacg cctgcgagat ttggaaaacg
//
LOCUS       DAZULY010000217         4189 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000217 DAZULY010000000
VERSION     DAZULY010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.220"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..2252)
                     /gene="topB"
                     /locus_tag="ACG81A_002420"
     CDS             complement(24..2252)
                     /gene="topB"
                     /locus_tag="ACG81A_002420"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS5015742.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            complement(2426..2836)
                     /locus_tag="ACG81A_002421"
     CDS             complement(2426..2836)
                     /locus_tag="ACG81A_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5015743.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            complement(2953..4116)
                     /locus_tag="ACG81A_002422"
     CDS             complement(2953..4116)
                     /locus_tag="ACG81A_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS5015744.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
BASE COUNT         1051 a          919 c          711 g         1508 t
ORIGIN      
        1 ttccgctatt tcattgtttt catttactct acttcgtttt cattattttt tcttgatgtt
       61 tctttgtttc catcaactgc ttttgttcca ctgttttcga tccatggaaa agaaaaaccg
      121 attgtcatat cttcttttaa tactaaaact gcatcgaatt tccctttttt accagtaaaa
      181 cctttgacaa ctttacttgt ctttcctttt gaaagcaaat ctctgagatt cgttttagtc
      241 agcttctttt tagctactgt tggccacaat ttgaagtcgc attccggatt caagcatgta
      301 gcgattttag gataaagcat gactattccc tttttacatt ttggacatct tcctaattga
      361 tcgtttttct tctccatcgc ttttatctga cgtgtcccgc taatctcatc tgaaaagtct
      421 accttgtcga tttgattagg aacttctgcc aataaatgta ggatgaattt tttgatattc
      481 tctaaaaaaa cagatggtgt cccctcttgt ttaccaattt ttctcaagta cccttcccag
      541 cgcgcagtca tttctgcgct cgtcagtaat ggttcattgg ctactgcctt acaaagagtg
      601 attccttttg gcgtaacagt tatttcgttt ttattgactt cgatgtaatg cttctgctta
      661 agattttcaa tgatattggc tcgtgtagct tctgtcccta ttccttctac ttctttgaga
      721 atttcttgcg cttcatcatt atccagcgtt tttcccgcag ttttcattgc tgtgatcaac
      781 gttccttcgg tatagggttt tggaggttgt gtttctttct ccatactttt cagttctact
      841 tctgcttctt cattttctac gataggagga agagtctgag cttctttctt ttttgacttg
      901 atgcgtaaga tttcctgcca accttgctgt aatgggatct ttccgatgct ttttagcttt
      961 aattgctgta cgcccgtcat tacggtcgtc tcttcgtaga tataatctgc agcaaacata
     1021 gccacagtag tgcgcaaaac ttgcaaatag attgcttgct gtaatttgga aagcttcgca
     1081 aagcgtgcag gagtcggaat cgtctttgtc ggaataattg catgatgttc ttgaactttt
     1141 ttcgcatcga catatcgttt ctttgctttg atctcagttg tctgtacttc ttctattcct
     1201 aaaaattttt tataattttg cagatgttta accagatact gatgctcatt ttctgtaatg
     1261 taaaaggaat ccgttcttgg atagcttaga aacttgcttt cgtacaatcc ttgagcagca
     1321 tcaagtgtag ctttggcact tgctttcatt agctgattca tctttgtctg caaactagat
     1381 agcgaaaata aacgtggact atgcaacctc ttttcctttt tcgtaacttc aagaatccgt
     1441 ccgttttgct tacctaaatg cgctccaagt ttttctattt ccttcgttaa ctcttcttgg
     1501 gttttaaatg ctttttttgg tacgattttt ccagaaaact gccctttgtt tgtcgtaatt
     1561 aaaccttctc cttcaaagta cgtttctttt ttgaagtgtt tgatttcttc ttgcagctga
     1621 taaatcatat agagtgtcgg tgtttgtaca cgaccaagag aaaaactgcc atcaatccct
     1681 tttcgttgaa gtagcaagct atataatgga cttccattca ttccaatcag ccaatcagcg
     1741 ttctgccttg cttgcgcttc tttatatttt ggaaaatagt tctttccagg ttggagattc
     1801 ttaaaacctt catagattgc ttctttttca agagaattga tccataaacg taaatagttc
     1861 ttttccttag aaaatgcgtt tgcttgtcga ataatcgacc acgcgatggc ttcaccttct
     1921 cgatcaccat ctgttgcaat aatgatggta tcggctgact gcaattctcg ttttacgatc
     1981 gaaaattgtg cacgtttgtc cattggaact tcgtaacgga aggtttctgg gaaaataggc
     2041 aaattttcta aagaccattt tgcccaacgc tcttcataag ctcctggagc aaccatatct
     2101 accaaatgtc caaaaccata agtgatggtt acttcaccag caaacaatgg atcgctgatt
     2161 tcataatacc cttcatgccg cttttgctgt tggaaggttt cggcataggc ttttgcttgt
     2221 gaaggttttt cagctagtat caccgtcttc atcttctgac ctccgttact taattttttg
     2281 cggacttctc tattatggaa catattttcg catctggcaa taaaaaaaag ataccgtata
     2341 gtcgaaatag acacaaaaac atcaagcaaa caaacgatga tccgtttgct gcttgatgtt
     2401 tccagaaaat attcatcttt agctgtcact cttggttaat ctgccagttg atgccaaatc
     2461 gatcagttac catcccatac aactgagccc acggaacagc atccagtggc atctcaatct
     2521 taccaccctc agctaagctt tcaaataggc gcttcgtttc ttcttcattt cttgtctgga
     2581 tgactaaaga aacattattt cccacaacta attcaggacc aattccttct ggcgtatctg
     2641 caaacataac taatgtatca taaatgttca gccgtgcatt cgtgattaga ttttttgctt
     2701 cttctggcaa gttcatttgt tctattccag gcatttcacc gtatgtggtg atacctgatg
     2761 cttcagtctg aaaaactttt tcataaaaag cgatggcttc acgcgactca tttttgaagt
     2821 tcagatagat atccaatgta aacagctcct ctcttggtta aatctaccat aagaaagcgt
     2881 tttttacgag tgaatcgttt gattgattca tgacaaaagc cttgcgcagc ttcttcttga
     2941 tcttttttat gatcatttat tcaaaaaatc tgtaatagca gattgattca tttgattatc
     3001 aatttcaaga atactgccag cataatcact ttgcccataa ttccacgaac cgtctactgg
     3061 cacacttatt cgatcaatgc caccagctcc tcgtgcaatc gaaaagaggt ttttcgtcaa
     3121 gaaccaagca gtgacatctg tggatgtata tcctaaagct ttaccagctg cataaggtgc
     3181 acggatcaga ttcaatggat tcttcaattg gctgaaaata gcgttcatta cttgctgttg
     3241 gcgtctgaca cggccaaagt ctccttgttc atccattcgg aaacgtgcgt actgcaaaag
     3301 aacatgtcca tccatcttct ggaccccttt tttaatatct acaccatcca gattcagatc
     3361 tttttctgca tcgatcttca ctccgttcat gaataaggca tcgatcactt tttcaaacga
     3421 tttgaaatcg actttcgcat agtattgaca atcgattcca aaattttcta ctaaagtttg
     3481 acgcactaga tcggctcctc cgtatgcata agctgaattg atcttatttt gtcctacccc
     3541 tgggatcgta acgaagctgt cacgcataaa agaaatcagt ttcggcttat gggaaggacc
     3601 atccaactgt aagaccatga tcgtatcggc tcgcccggca tcttctccgc gtgtatcact
     3661 tccaaggatc aaaatattat gcgcaccatt cgtcgctttt tgcccatgaa aggtttctga
     3721 agtcgctgcc tctaagcttg tgtcatgttc agctacatat ttaccaataa aaaatgagat
     3781 acctgaataa aggatcaaaa caagcaaaat cgatagaatg atttttttcc acgatcgctt
     3841 tcttcgaggt tttaacggtt tgttcttctt ttgccgattg aaaaattttt tatttttttg
     3901 ttgttcttct gctcttggtg catctctgta tatctgttgc tcgtattggt tcaaatgttc
     3961 aaatgaatgc tgctctcttt cataaatacg ggtagcatct tcctcatttt ccatacgcgt
     4021 tcgttttcgt tctcttctag caaaaatact ccccgtatcc cgcttcagag gtttcgcctt
     4081 cttatggatg tccttatagc gatccatccg actcattgtg gttcctcctc gtttcttact
     4141 gataaataaa ggatacgata accaatcgac aaaagcctat cattttttg
//
LOCUS       DAZULY010000218         4182 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000218 DAZULY010000000
VERSION     DAZULY010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.221"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..1842)
                     /locus_tag="ACG81A_002423"
     CDS             complement(76..1842)
                     /locus_tag="ACG81A_002423"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5015745.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1802..3555)
                     /locus_tag="ACG81A_002424"
                     /pseudo
     CDS             complement(1802..3555)
                     /locus_tag="ACG81A_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(3665..4180)
                     /locus_tag="ACG81A_002425"
     CDS             complement(3665..4180)
                     /locus_tag="ACG81A_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFS5015746.1"
                     /translation="MYFFSIMVSSIGGAFGHSISPGIFGLLSGIIFVYISYNYPRKGI
                     FTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRAIPQVASWALMQLG
                     SAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLVVASLSMIGVL
                     IGRKVLKKYKK"
BASE COUNT         1414 a          793 c          587 g         1388 t
ORIGIN      
        1 taataaaatt attctttttg aaaaagaatt ataactatat cccaaaaatt gtaggttaat
       61 ttaaagccta tttcatcatc gtgattgagc ttctttttca tagacgaatt tctcagatag
      121 tttagttttc tttatgagat ttttgtatat ttctgacctt cgatacaact cattatgagt
      181 accttgatca actagttttc cttgatccat cactacaatt ttatttacct tttcaataga
      241 cttcaagcga tgcgatataa ttagtactgt cttatcttgt attaaacgat ttaaactttc
      301 ctgaatacgt ttttcatttt ccgcatctaa actggcagta atttcatcta aaattaaaat
      361 tggcgcattt ttcaaaaaag cccgtgctat ggataggcgt tgtcgctctc ctccagaaag
      421 tgaacttccg ttttctccga ccaatgtatg ataaccgtca ggtagttttt caataaagtc
      481 ttcgcagtta gctaaacgag ctgctttttt cacttcctca tctgacgctt gactatttcc
      541 aatacgtata ttttccaaaa tacttgtatt aaacaaagtt acctcttgaa aaacaatagc
      601 gattttgcta aacaaagaag ctggagatac acgtttaata tcatacccat caattaaaat
      661 acagccttca tcataatcat acagtctaga cactaatttc aagatactag ttttcccgga
      721 accactagca cctactaacg cagtcacttc tccttgttta gccgtaaaag agatatgatc
      781 taaaattggg gtattgttat catatgagaa agagacatct cgcaactcaa tgtcaaaaga
      841 tttcagagga ctatctgatc cctcttggat actggtttcc ttcatagctc taattcgctg
      901 tattttagga gctaaataaa atatttcaag cactgcttct ttcatagaat cgaaggaatc
      961 ctttattttt attgcggcta gtaagtaacc aacgacgtag agtatagtca cttcacccgt
     1021 taacagtaag tgaacaccta ctagaatgac tactgctaaa ctaacatatg agaaaataga
     1081 ggataaggcc atgactgaaa aagtacttaa ttctaccttt aaatgaatcc tttcactctc
     1141 ttccattttt ttgaaaagtc tgtcttgaac ttttttagat agattaaagc tattaatctc
     1201 ttgctgcaat tctatagttt cttgaaattc ttccgagttt tctctcaaag tatcgtaata
     1261 tttagtattg gctttcgttt gggatttctt tgataacaag attaagacaa aactaaataa
     1321 cgttggcaaa ataacagcta atcccatttt gacattacca accaaaagca tcactgaaat
     1381 aaaagggaaa aacagagcca taccgccggc tttaggtatt gcatgactca tcgcatgctc
     1441 aataccttca acgtcagaca tgatagtttg agataaatct gataaattat gttttgaaaa
     1501 ataggataat ggtaaatttg acaaatcgtc tgcaatttgc actcttaaat gcgcactttc
     1561 tttataagta ctattatata agttttcata ctccctatct aacaaccaat acatgaaaag
     1621 caaagtaaca aacgatacgg caaaataaag ccaacggggc tttgtatttt ctaaaactaa
     1681 ttcgtctatc agcatcatta taatgaatgc tggtaccata ttaatgcagt atacaaaaaa
     1741 actagaaata gaagctttag ttaaagcttc tgcgccctga tcacttaaag caaatttgtt
     1801 tttataaaaa cttttcattg tcaagcctcc aatcatttgc attctcataa ttagtgacca
     1861 attgtttata cacttgatgt ttagccatta attcattatg atttccacgt tctactattt
     1921 tcccttctga caagacaaga atttcatcta cagatttaat gctggataat cgatgagcta
     1981 tcataataac tgttttttct ttcatcagat gttgaaaagc cttttgtagt tcatactcat
     2041 tatctggatc aatagatgca ctggcttcat ccataataac aatctttgca tcttttaaaa
     2101 ttgctctggc aatagctata cgttgttttt ctcctcctga cagataaact cctttactac
     2161 caattaaggt atgctctctt tcgggaaatt tttctaaaat cgattcgcaa ccggctaatc
     2221 ttagcgcgtc aaatacttta cttgctggcg cttgaggatt tgctatagaa acgttatcgt
     2281 aaatactctg attgaatagt ttcgtatctt gaaagacaaa agcaattgtg ttaataattg
     2341 tgtcttttga atagtgattt aaaggcttac caccaatttt aatctgtccc ttatccacat
     2401 tgtagaaacc tgaaattagt ttagctatcg tactctttcc gcttcctgat tttcctacta
     2461 atgcataaat tcttttttgt tctaaaacta agttgaaatt ttctaaaaca cactcttctt
     2521 tgtatgaaaa gctgacattt tcaaatgcaa tgtcacaatt tttaatctgt gtctcgttcc
     2581 catattttag ttggtcttgc ttcatttcta aataaaggga gtctagtgtt tcgactgcat
     2641 atcttgcttc aaacaagtgc atacttacat acattattct catgaaagaa acaaaaataa
     2701 ttccacttaa aaaaaacaac atcaacaagt caacagctaa tatcttggga gtggtaagag
     2761 aagttataaa aaaggttata ggaactatca aaatgcaaac tatgccaaaa aatagccatt
     2821 gaaaaagaac aaaaggtgtt ttcccactca aagaatactt atatgcaaac ttagcataat
     2881 cgttaattgc tttattcaag gctttaaatg atgttacttt agcaccaaat atctttataa
     2941 cttgaattcc gcggatatat tctaccgttt cagagcttaa cacatctaac gattcttgat
     3001 atttttgaat aaaatttgta ttctctgtca ttttctttaa caagaataca ccaattacta
     3061 acatggcgat tagaaccaat ccaattttaa agttaatgaa aaagcctaag gcaactgata
     3121 aaacaggtgt tactatggct tgagcactat ctggaatcaa gtgagcaatt gccatatgtg
     3181 ttttagccgc gttgtcatca attgtctttc gaatatatcc cgaagaattt aaatcaaaaa
     3241 aacgaaaact tgcttctgtt aaaccgctaa ttcccttttt tctcaaatta gtctctaagc
     3301 gaaacccaag gatatgagaa aataagccag aaaaaatata aaataaagct ctaaagttaa
     3361 ccaagcaaca gtttgaaccg cataactctt agctaaatat tcatctccta caataattag
     3421 agactgaaga aatttgtaaa tactgtaata tccatagact gttaatacga cagaaaaatc
     3481 agaaaataaa gtggctaacc atccgtagta tttcatttta gggacataac taaataattt
     3541 tttgtatact ttcatttttt cctcccccgc attcaatttc ttctcttatt cgtttacaac
     3601 tgttaatgct ttccgtaata gcgtaaatta gagatgtaca aacagataaa aggtaaacta
     3661 cgttctattt tttgtatttt tttaagactt ttcttccaat caatacacca atcatagata
     3721 aacttgctac aactaacact gaaatgattc cccatatacc taccgcatag tggatcattg
     3781 catccattgt tgcagcagat tgtcctttat ctatccaatc ttgacgaaaa cgatctgtaa
     3841 agaaccatat aggaatccac tgtccggcag cactgcctaa ttgcattaga gcccaagacg
     3901 caacttgagg aattgctcga tcatatccaa atacagatag aataatatca gctaagatcg
     3961 ccatactaat cgaactgata aaccaaggta agtacgcccc gcccattaac gtaaaaaata
     4021 acaataaaac aattgtgtaa atagtaaaaa ttccttttct agggtagtta tagctaatat
     4081 aaacaaatat gattccagat aataatccaa aaatcccggg ggatatgctg tgaccaaacg
     4141 ccccaccaat actactaacc attattgaaa aaaagtaaat ta
//
LOCUS       DAZULY010000219         4159 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000219 DAZULY010000000
VERSION     DAZULY010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.222"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            15..125
                     /locus_tag="ACG81A_002426"
     CDS             15..125
                     /locus_tag="ACG81A_002426"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015747.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
     gene            130..2259
                     /locus_tag="ACG81A_002427"
     CDS             130..2259
                     /locus_tag="ACG81A_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5015748.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            2256..3263
                     /locus_tag="ACG81A_002428"
     CDS             2256..3263
                     /locus_tag="ACG81A_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFS5015749.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            3280..>4159
                     /locus_tag="ACG81A_002429"
     CDS             3280..>4159
                     /locus_tag="ACG81A_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFS5015750.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYK"
BASE COUNT         1371 a          672 c          946 g         1170 t
ORIGIN      
        1 aaaacggtca atgcttgatg caagacttgt atgggcgagt aggtgtggtt caagttcatc
       61 cagtctttga agaactcttc catgcttttg acactcgtcc gccggttcaa gagagaatgg
      121 agtgagggga tgaaaaagaa actttttcgg attgtgggaa tcgtttttat tagcatcgtc
      181 actctgacag ttctattgag tttagtcggt acggtagctg aggcgacagg tctggttgat
      241 gatacagttg aagccggaaa cctttattcc aaatattctc tgaacaatta tcaactggat
      301 ttctttgtgg atagttcttg ggattggtta ccttggaatt ggggggatgg cttagggaaa
      361 agcgtgatgt atggtcttta tgccattacc aattttattt ggacagtaag tctgtattta
      421 tcaaacgcca cgggctatgt ggttcaagaa gcttacaagt tggatttcat ttcggatacg
      481 gctaaaagta ttggaaagaa tatccaaact ttagccggta tcactgaaaa tggattacag
      541 acttcgggat tctactttgg gttcctatta ttgatgattt tagctctagg agtttatgtg
      601 gcgtataccg gattattaaa acgagaaact accaaagcag tccgaaccgt cattaatttt
      661 gtgatgattt ttctactctc gggttctttc attgcgtatg cgcccaccta tattacaaaa
      721 atcaatgact ttagctcaga cgttagtgaa gcggctctta gccttggaac caagattgtt
      781 gtacctaatt cagaaagcca gggaaaagat agtgtggatt tgattcgaga cagtttattt
      841 tcgattcaag ttcaacaacc ctggttacta ttacaattcg atgattccaa tatagaagaa
      901 attggcgagg gtcgggttaa taagatttta tccgtgagtc cggatgaaaa taagggtaag
      961 gatcgggaag aagcagtcaa agcagagatt gaagataatg acaatgcgaa tctcagtatt
     1021 accaagacca tgaaccgttt aggaaccgtg gtctttttag tgttgtttaa tattgggatt
     1081 tccttttttg tgtttcttct gacagggatt atgttgttct cacaaatcct tttcattatc
     1141 tttgcgatgt ttctgcccat tagtttctta ttgtcgatgt tgccgacgta tgagagtttg
     1201 ggcaaaaggg cgattattcg tttgttcaat acgattatga tgcgagcggg agttacctta
     1261 gtcattacga ctgcattcag tatctccacg atgtttttca atatttcggc cacctatccc
     1321 ttctttatgg tagcttttct acaaattgtg acctttgccg gtatctattt caaattaggt
     1381 gacattatga gcatgtttaa ccttcaaagt aatgatagtc agtcaatggg aaggcgagtc
     1441 atgcggaaac cgcaaatgtt gatgaatcga aaattgcgac agcttaatcg aaatgtgggt
     1501 cgaacttttg cttttggtgg cggtgcagca gttggtggga aactagctac cagttctaca
     1561 aaagggaaag gtagttcaaa aatcaataga gtgtccaata gaaagaacca ttccgcgaga
     1621 agcattaacg agctaaattc tgatctgaaa aaacaagaac aaccagtggg cacgaagaaa
     1681 gaatcacgaa taaatttagt tggtagaaaa gttggaaaag tactcgatac aaaagaaatc
     1741 gtgaaggaca aagcgaaaca agcaaaaaat caagtaaccg atgcaccaac taacttgaaa
     1801 tacagccttc ataaaggcat ggaaaagacg aaaaaagtac cagaggactt taagcgaggg
     1861 cctttcgaag aaaaagcaaa tcgaggggag ttgcgagaaa aacaacgaca acgaagagac
     1921 gcaaaaatgg atgaaaaacg caaagtattg aacgaagcat ggaatcgtca tgagaaagga
     1981 agggaaaatg ttccgataaa aaaggagata aaaccacaga aaaatgaact accagaaaat
     2041 aggtctttaa aaccaagggt acaagtacat tcgaatcctg aaatcaaacg gaaactttct
     2101 agtcaagaag tcattccaaa cggaagcact cagccactaa gcaagaacaa ttcctttcaa
     2161 caagtgaaac agagattcac ttttccaaag cgaaccaatc aacaagtaca aagaaaaaag
     2221 aacactcgcc caaaaccaaa gccaggtgag aaaagatgaa cttaaaaaaa ttgagtatta
     2281 tttcgttaac cttcctgacc atctcttttg tgggcatctt gatgtgtgtg ggtttgttgt
     2341 ttggtgagga cggtgaggga agtggtagtt ctggaacttc tcctggaggt agtagtgtct
     2401 cagaagtagt tttgaaacac cgttcaatgg tggaaaagta tgccaaagaa tcagatataa
     2461 cagagtatgt ttcgattctt ttggcgatta ttcaagtcga atctggaggt acaatggaag
     2521 atgtcatgca aagttctgaa tcagcaggtt tatcaccgaa ttcattaagt accgaggcat
     2581 ccatcaaaca agtgtgtctc tatttttcgt ctttggttaa gtgttccaaa gaactaggat
     2641 gcgatcaaga tagtatcatt caggcttata actatggtga cgggtatttg gattatgtcg
     2701 cgaaaaacgg aaagaagcat agttttgctt tagccgaatc tttttcaaaa gaaaaatcgg
     2761 gtggtcaaaa agtcgattat cccaatccca tcgcgattaa ggaaaatagt gggtggcggt
     2821 ataactatgg caatatgttt tattgcttat tagtgaagca gtacctaact acaacacagt
     2881 ttgatgataa aacagtccaa ggaatttttg acgaggcgtt caagtatgaa ggaacggctt
     2941 atgtatttgg tgggtcaagt cccggaactg ggtttgattg tagtggttta actcaatggt
     3001 gttatgccaa agttggtttg aaactccctc gggtggctca ggatcaatat gatgctatga
     3061 ctcatattga cttaaaagat gccaaaccag gcgatttgat tttctttcat tcaacttatg
     3121 atgccggtac ttatgtgact catgtgggca tttatgcagg agagaacaga atgtttcatg
     3181 caggagatcc ggtgggttgg accaatctta ccgatagcta ttggcaacag cacattattg
     3241 gtgctggtcg gtataaacaa tagaaaagag gaatggaaaa tgaagataaa aattgagcga
     3301 agtaacaagg aaaagattcc aaaaaagaag aaagaacgag tagtatccgt gggcaagcat
     3361 cgaaaaatgg tgcttgccct ttggctttta ttgtcttgta gccttgcttt tggaatctac
     3421 aaaaacttca cggccattga tcagcataca actcatgaga aagtggtcgt gaaagagaaa
     3481 atagtgaata cctctggagt agaaaatttt actgtagatt ttgtgaaaga atatttttca
     3541 tggaagaata acaaggaggt aattgaaaag agaatgacta atcttgagca gtaccttaca
     3601 gaagaagggc tcgctttgag ccaagatatg gttcgagcgg atattcccac tagttccgag
     3661 gttgtatcag taaaaattct agatgttgaa aaacactcgg aagaatttgt cgtttcattt
     3721 gtggtagatc aaaagattac agaagggaaa aagacacaat caatttcttc tgcatatcgt
     3781 ctgatgatct ttgaagatga aaaaggcaat catattgtaa cgagtttacc cactatgatt
     3841 ggtaagccag aaaaggcaaa acataaagta agacaagtaa aaagtgattc aaaaattgat
     3901 tcaaagacaa cggaagaaat caccgagttt ttagagacgt ttttcaggct ttatccagca
     3961 gcctcagaaa aagaactaga gcattatgtt gaaaaagacg cgatgcaacc aattaatact
     4021 aatttgaaat ttgtggaatt agtgaatcct atttacaaac aaaagggtaa agcgattcaa
     4081 gtgattgttg gtgttgagta tcttgatgag ttgtccaaaa caacaagtgt ttttcagtat
     4141 agattaaaac tatacaaaa
//
LOCUS       DAZULY010000220         4061 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000220 DAZULY010000000
VERSION     DAZULY010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.223"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(108..980)
                     /locus_tag="ACG81A_002430"
     CDS             complement(108..980)
                     /locus_tag="ACG81A_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFS5015751.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            complement(984..>4061)
                     /locus_tag="ACG81A_002431"
     CDS             complement(984..>4061)
                     /locus_tag="ACG81A_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS5015752.1"
                     /translation="NQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLAD
                     NEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVKN
                     LLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNIF
                     PSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHPG
                     GAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYADG
                     IGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDSL
                     SEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGATW
                     NKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYGS
                     FIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMVG
                     GIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHEE
                     YVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDLL
                     DDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDGA
                     VNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRYT
                     AAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMGS
                     GDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGND
                     GLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIGK
                     GHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKSE
                     INSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
BASE COUNT         1172 a          821 c          651 g         1417 t
ORIGIN      
        1 attacttact tcttctccaa tttcatccgt tccaaattcg aactcagtcg aaattaaacg
       61 ttcgttatat aaattcatat ctcggtgaaa gagggtaatg gctaaaatca aaatagatca
      121 cgctcctcat aaagttcaat ctctacattt gcatgtccaa catgaaaaac aacgctgaat
      181 tctcctagag gcgcttggac gaagtttgat ttagtaaaat cttgttgttg ataaacagaa
      241 gattcaactc cacctacatc ttttaaaatt gccgtttggt cttctagcaa actagacact
      301 tctaatattt gtgtgtcttt catattaata aaatagccat ctgaagctac tactttagag
      361 ttttggataa cttcccaata tggatttgag cactctccaa taatccttat ttttaacgga
      421 gacattcgct ctgtactatt tgtcaaatat acagaattat tactgaactt aaatacacct
      481 ttcttttccc atacattttt ggtgtagaca tatggtcgaa caaatgagta aacttttcca
      541 cgtgtattga gagaagttga attctgtatt aatttttcac gcttaataga ataccagctt
      601 gaagtaaaat atagattcaa agtatctgtt agcaatgagc tttgggaatc aatttctgtt
      661 ttagataatg atttcaaact acatttcctg atcattgttt ctgaatcaaa agaaaattct
      721 agttcaaaag gtccattagc cagaaattca actaatgaac catataattg ttgttctcta
      781 aatccgtgaa cagaaatcat aactgatgat tgaaattcag acaattcaat actctctcct
      841 atttctctga aactccccca ttgtccaata tgctcttttg taacttcaaa acctagattc
      901 gacatttcag tagcaaaata atcttctgtg gacaaatcaa tttcttcatt caatttattt
      961 cttaatatta ctgtcctcaa atactatgtc ctcctgatta aaattttctg aatatctaaa
     1021 gctagttctt tatctgatcg attgttactt ttaaaaaacg agagtaataa tgatagcaga
     1081 tcatttgtca tacgagactg tttttctatc gcttgtagga tatgtctatt atctacagaa
     1141 ttaatctcac ttttcgtttc ttttttattg cttccagaaa ggaaggctaa tgcttgaccc
     1201 ataagttcga tagctcttgt ttttcttgtc aatggaataa ccatttctgg tttatttcct
     1261 tctcctgctc gatatagtcc gtctttggta atcaagccgc cattggcata tccatgtcct
     1321 ttcccgataa cttgcaacat acctgcaact ccgtaacgtt tcttagcata attgattgct
     1381 gctaacatgt tatcaaatcc gttcataata tttccgtgac caggaaaagc gttagctgca
     1441 aaagttccgg gtttcgtttg aagtaatcct gttgcattgc cttcggcgag cccatcattg
     1501 ccgccgattg ctttctcatt accaccagat tccgtttgga tttgggacat ccaggcattt
     1561 atatacgctg ctgtagctgg taatccattc attttcaatg ctttagacac ataagaacgc
     1621 cagcgtgcaa caccacttcc tccaactcct ccattgaata tatcgccaga ccccatcgaa
     1681 ccgtttaaat gaatatgatc gaagtgatcg ccatctggcc aattcgtcca ttgtccgctt
     1741 gaccttgttc cagataatcc catgcggtca cgtacacgtc cattcgtaat gacatacgca
     1801 atttttgaag ggaatttttc aaaagcgtaa tttgctgctg ctgtatatct cggatctcca
     1861 gaaatacctg gataagccaa gtcgatagct tgccgttttc cgtgatagta ggcatctcct
     1921 gctcgatatc cagaagttac tgtaagtccg gggaacttac ccattacttt ctgtgccaca
     1981 tcaactaaat attgataaac accattggca tttactgcac catcaaaatt gccatgagtg
     2041 aagaattcac ttaattttga ttgaagcatt gtatttgcag ctttggacat taatgacgtt
     2101 cctgatttag tcatatcaag ccatggttca ttaatgcctg aaaaatcaac tttgctagtt
     2161 aagaattcta acattctttt ttcatcatct aacaaatcaa caatatctaa attgccaata
     2221 cctttttcgt agtgtggaat atttaaacgt tcctttaatt tctttgttaa agaagcattt
     2281 aagacctgag cacctttagg caaattaaca agaagatctc tacctttggc aataaaacta
     2341 cgtccatctg gcatctgaac atattcttcg tgaactaggc ctttttgatc attaatcata
     2401 gcaagtccac caggatgtcc atcagttccc ttagcgtatt gtggaattgg ccagtttccg
     2461 atattcttac ttgattcgac ttccttaagc acatagttaa ctcccgatat gacgccatta
     2521 actcctttgc ctataccacc aaccattgta ttagcgacat tgttcattgt agtagaaaga
     2581 gaattaccta acgaattcat tccgtttatt aaagattgca acaagaatgt ccctgcattg
     2641 taaaaaccac cacttttaga acgaaggttg ttaatcgaat cgttaccaag ctgatttaca
     2701 cgggctatga atgatccata caatgaattc caaccattaa gattattttg ctgccacgtt
     2761 cggccattgt tgtacatagg agtgttgtag tttctaagcg tgaccattgc ttgattacaa
     2821 aatgaattga ttgtagcaat aaaggtccca gttaaactat tccatccatt cagtaagttc
     2881 ttattccatg ttgctccttg cgtataatta ggattgtttt gaagttttag cgcattgaga
     2941 tagtttgtaa taaaaaccat ttcactattc atatattgag gaaccgctga gttccaacca
     3001 ttcattagat tagttaacca ttgagctcca attcctagat actgatctga tttttcagaa
     3061 agactgtccg gaataatcgg atcggcaact tttgtttcat ctgatagttt agtatcttta
     3121 gcatcagtcg ttcctgccat cagttgagtt ctcaataccg tcgttagctc gttaatagct
     3181 aaaattaaag catctaagtt tagcgtagat gaaaccgtcg ttatgtttcc tataccatca
     3241 gcatatttag gcactagacg tttagtttta gtggcgttca gtactttcga acctcttggt
     3301 aagtctaaaa cgacattacg tcctttaggt ataaaagttt ctccgcttgg caaggttata
     3361 agctcttcat aagtaggccc tttttgatcg tttaccatag ctgctccgcc aggatggaag
     3421 tttgttcctt tcgcattttt cggcatagga cctataaagt cagctgatac agttttagta
     3481 attttatctg gtatttttgt ttggaagatg ttgaacgctt caaatgcatc tttagcagct
     3541 tgtgaagcct catcgtgtcc tactgcagtt ttatcactag gaaaaatatt tttgttgtaa
     3601 ttctctatag agcttttagc cccactgatt gcctctttta aattaacgtt atctccatat
     3661 agagttttaa gaagtggcat aactttgtta tattcttcta cacttacagt cccgtctttc
     3721 actttagcca gtaaatcaat attattacca agcaagtttt taactgtgtc tggaattgct
     3781 ttccatgcat tccatgattc ttctgaagcg aatattttgt ttgctaagtc agtattgtca
     3841 gctaacatat atttttgatt atctggtaat tgagtccatc ttccgtatat ttcttctgaa
     3901 gataaaacag tggtagctaa atcttcgtta tctgcaagta atcttttctc attatcagga
     3961 aggtttaccc attgattcca cattctgtca gatgataaaa gaacctctgt aaatttagta
     4021 ttattagccg aaagaaactt tgcattagat gataactgat t
//
LOCUS       DAZULY010000221         4045 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000221 DAZULY010000000
VERSION     DAZULY010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.224"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..845
                     /locus_tag="ACG81A_002432"
     CDS             66..845
                     /locus_tag="ACG81A_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFS5015753.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            838..1155
                     /locus_tag="ACG81A_002433"
     CDS             838..1155
                     /locus_tag="ACG81A_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFS5015754.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            1399..3009
                     /locus_tag="ACG81A_002434"
     CDS             1399..3009
                     /locus_tag="ACG81A_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS5015755.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            3166..4014
                     /locus_tag="ACG81A_002435"
     CDS             3166..4014
                     /locus_tag="ACG81A_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFS5015756.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         1177 a          705 c          827 g         1336 t
ORIGIN      
        1 aatttagcga atttatgtta caatgagtcc gctgtttatt tgttgtaatt aaaggagata
       61 ttttaatgaa caaaaagaaa agtgcgccat ggcttcaagc tttgcatgca gtgatgccac
      121 tttgtataag ctatatacca gtaggattag cttgcggagt attgttgcaa aaagttggat
      181 ttgatcctct cttatcagga ctgttatcga ttctggtctt ttccggtggc gcgcagtttt
      241 tagttgcatc tatgcttacg acgcaagcat catttgcaac gacattattg atggttttct
      301 ttttagaatt acgctataca ttattaggag caagtcttgc cggttttatg aaaaaagaaa
      361 agcggacgtt tttggctgtc ttttctcaat cactgaatga tgaaaactat gcggtgaact
      421 acttaaagtt ttcaactgac ccttcctgga ataaaagaaa ggctttatat gtcaattggt
      481 tctcaatgac tgcatgggcc ggtagcaata tgatcggtaa ttttttcggc tcggtgattc
      541 gagtagatgc tgaccttgtc cattttgctt tgacggccat gttcattttc atgtttatca
      601 tgcagatgaa aagcgcacta ctgatcttta caggtttatt ctcaggcgta ttaggggtcg
      661 tattcatggt attattccaa aatactttcg gattgattgc agcaacagtc attgcttcat
      721 ttgccggatt tatgctggag aaggcattca aaaaagaaaa agctaagaaa ctaagagacc
      781 gcggtaaaga tgtcaatgag ccgctcttca acttcccgga acaggaggcc aatcaccatg
      841 actaaactat atttgctact tttagttgct tgtctgttca tcgtggcata tatcccgcgc
      901 ttgttcccga tgctttattt tactcatcga aaagtgccat cctggttcag tgactggatg
      961 aaatatgtac cggtggcatt atttgcggcg ttagctttta aagatgtttt cattactcat
     1021 gaacatctgg atattgcttg gaatatcaaa atagctgcaa tgattctagt agcaggcgtg
     1081 gcttacaaaa cacgttcgat ggcgttgtca gttttgacag gacttgcgtc tgtctttttg
     1141 ctgtctatgc tttaaactgt tttattttat taaaaacaga gattgcgaca taagttgttc
     1201 aaactgatct atccgaactg catttgcaac agtcaattct ggatgcaagt ttattcgtag
     1261 ggataaaaaa gattagtgtt ggcttatgat gcaagctctt ttttatttta ggaattagag
     1321 agggatttgt ccttctatga ttttttgcta taatagggaa gatgtttatt ttatgaaaga
     1381 atagttgagg gaatttaaat gaataaaaaa ttgatgcaag gaacattttg gctaaccttt
     1441 gctaatcttc tatgtaaagt tttaggggta gtctatttga ttccctggct aagtatgatg
     1501 gggaacaatc aagatggtat gcttgctacg acattgtata atgttggcta tcttccttat
     1561 ggattgttcc tgatgcttgg tacagtcgga tttccaaatg cgattgcaaa aaaagtagct
     1621 gtagccacaa aagaaggaga taacgctgct tgtcggctga tttttagaag tacgatcaat
     1681 atcatgtttg tcatcggtat cgcctctgct gcactgatgt atttatttgc tcctttacta
     1741 gccggaatca gtccgattgc taacgttaat aatgggatac tagctatcag aagtttatgc
     1801 ccatctttga ttgctatccc aattcttagt gcaatgagag gatatttcca aggaaaaaat
     1861 gatctgcggc cttatggaac ttctttgatt atcgaacagg ctgtgcgagt gatcgttatc
     1921 ttggctggta cttattactt gcgcgtattg actgacggaa ccatattaga agctgtattg
     1981 atcagtacag tcgcttcttt cttcggtggg ttagctgcga ttatccatat gtatatcgtc
     2041 ggacgcagaa aagacttctt cgaacttaaa gattttttga tttccagccg ttatttcgaa
     2101 agagaaaacc gatctgcctc tgtctctatc attcgggaaa cactaccgtt tatttttgtc
     2161 ggctcagtca ttactatcgt tcaaatgatc gaccaagtaa cgatgaaacc actgcttcac
     2221 tttttccgtc cagagatcgc agatcagcaa ctggaattct tattcagccg tgcttctgtc
     2281 aatccaaata aattgacatt gatcctgatc tccatggtag gaacagtagc tataagcagc
     2341 ttgccaatat taagcacgat gaagaaaaaa gatcggatac aaatcgaaaa aacagtagga
     2401 gatagttttt cgattgcgtt attgattctt cttccttcat tgactggaat gtctttatta
     2461 gctggaccac tatacacatt gttttttggc tatgatcctg aaagtgtcgg atatttccaa
     2521 atggctttgc tagcttcttt gtttttctct ttattcacga tcctatctac gatgcttcaa
     2581 tctttgaatc accatcgttt tgcgatccga ttaactgtag aagcaattat cctgaaagtc
     2641 atcttccaag taatcggatt aggattagtt ggcggatacg ggatgagctt atcaaatggc
     2701 ttagcattcg gtattgtctt tgttcgcggc tatcacttta tgtgcaaaga ataccgaata
     2761 gcacctttag caaagatcag caattttttc ttaaaaactt tccgctccac cctgattatg
     2821 cttgcagtat gttttgccgt tttctttttg ctgaatcagc gattgtcaat ggaatcaaaa
     2881 agctatgcaa tgatttattg tgtggctgta ggaagtattg gcggcttagt atttttattt
     2941 tctcaattcg gaaaaaatgg gtttcgaatg ttgaaaaatg ttaaacatca tagcagaagc
     3001 aaagaatagg ttaactaatc gttttttgct gtcacttgaa aaacggcaac tcgtatgatt
     3061 acgcttgtcg tttttctatt ttccaatttt agtttcatat ctaatcttac ttatgatata
     3121 gttataaggt ctggaaaaat tctttgaaac gaggaaatta aacacatgaa acttgcagta
     3181 gttacagata gttcggcata tctgccggaa cgtatcaaga accatcccga cttattcgtt
     3241 attccaatcc ctttgattat ggatgggaaa atttataacg aaggaatcga tatcgaagcg
     3301 gatgaatatt atggactttt aaaaaacagt aaagattttc ctactacttc acaacctgtc
     3361 gtaggggaag ttttgggttt atatgaggaa ttgaaagaga aagggtatga cacgatttta
     3421 agtatccatc tttcttcagg gatttctggc tttgtcaaca cattatttgc gatgaaagat
     3481 gacatcagcg gagtaacggt cattccttat gactctaaga tcactagtat gccgatggga
     3541 cacatggtag aagctgcctt agacttaaat gatgaaggtc agtcccttga tgaaatactt
     3601 gcacatatcg atcgaatccg agataatacc tatgcttatc tgattgtaga tgatttgaac
     3661 aatttagtac gaggaggacg tttgacaaat ggtgcagcgc tgattggcag cttattaaaa
     3721 atcaaaccga tcctgacatt ttctgaaggg aaaattgttt tattcgaaaa aatccgttca
     3781 agtaaaaaag catttgctcg agctgaagaa attattggta gaagaaataa cgaaatacaa
     3841 ccgccagtca aactttacgt gatacatgca aataatttag aagtagcgat tgcggagaaa
     3901 gagaaattgc agaaaaaata tccaaatgca gaaatcgaaa ttggtcattt cggaccagtc
     3961 attggtacac atttaggtga aaaagccatc ggcattgcca tttcagcgca ataattcctt
     4021 ttattcaaaa aacataccaa aaata
//
LOCUS       DAZULY010000222         3976 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000222 DAZULY010000000
VERSION     DAZULY010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.225"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..1591)
                     /locus_tag="ACG81A_002436"
     CDS             complement(86..1591)
                     /locus_tag="ACG81A_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS5015757.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1588..2274)
                     /locus_tag="ACG81A_002437"
     CDS             complement(1588..2274)
                     /locus_tag="ACG81A_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5015758.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2467..3888)
                     /gene="gndA"
                     /locus_tag="ACG81A_002438"
     CDS             complement(2467..3888)
                     /gene="gndA"
                     /locus_tag="ACG81A_002438"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFS5015759.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1095 a          848 c          684 g         1349 t
ORIGIN      
        1 aatgacatcc ctgaatttct cacttgcttg tattttgtac cttattcttt gttggatata
       61 taaaaaaaca agtaaagcac atgctttact tgtcttctgc ctttgcgata ggaataaaga
      121 ttctaaaaat tgtcccttgc cctactacac tttctgctaa gatacgtcct ttatagtttt
      181 caaccagttg tttagcgata gacaacccca gaccatttcc acccttcgtt ctagctcttg
      241 ctttatccac tcgataaaaa cgattgaaga tcttattaag gtcttctttg ggtattcctt
      301 cgccaaagtc ctgaatcgcg atctcaaatt cattcagagt ggaagaaata gagatatgga
      361 cttcttttcg tgtagttgaa tactttactg cgttatccaa tatgataatg attaattgtt
      421 caaagtgatt acgataaatt tgcagggtca cttcttttgt caaatcatca tctaaagtga
      481 tcacatagtc tggatataat atcttgaaat tattataaac ttgataggtg acttcttttc
      541 ctttagttgt ttcattcgca taatgaacat ctacttgttc tgctcttgat aagtctagca
      601 tctcttgaac taagcttttc atacggctga tttcttgcat gctggcgctt aatgattctt
      661 ctaatatctc aggatcatct ttcccccaac gatttagcag actaagatgc ccttcaataa
      721 ttgcaacagg tgtccttaac tcatgagata cgtcttccac gaattgctct tgttgttcaa
      781 tatacaaacg catacggtca agcatctcat tgaatatttc agctaaatct gccaattcat
      841 catttgtgtc gatttcaggc atgtggatat ctgattgcgg atctttacgt atagtatcca
      901 ttgtatctct gagtaccttg agtggtttta agaaataaga agataaaaag aaacctaaaa
      961 tactacttaa gatcaaagaa acaacttcta aaacaataag agtcaataat agcttatttc
     1021 gaatatcata gaaagaagat aattcataga agctttgcgc atagccgatc ttttctcttg
     1081 tacctttaga atatataggc tggatagata aaaagccagt ttttccttct agtgtaacga
     1141 tcgttggaac tttacgtgta gtctgaatca ggtttcgact gttttcatgg gttttaaaaa
     1201 tcaagttctc gtccaaatca taaacataaa gatccattga tggctgccct agctcagcta
     1261 taaaggaatc gatcttcatt aagtttcctt cgaccagtga agtatgatct acgacctcat
     1321 ctccgtcatt tgtcgctttc gtcagattac gatacgtatt tactaatgtc agttcttcat
     1381 ccgagttagc caaacgagaa gctacttctg aaatcgtacg ctctacattg tttctttctt
     1441 ttgccacaat cagattgata gatgatttat aggtaatgac agcgaaaatt gtaaacacaa
     1501 cgaatatgaa gaaagaactc gcaaaggccc acttgattgt caaggagggc cctttcagtt
     1561 ctcttttttc cgattttttc ggctttatca cgagcgcatc acataacctg ttccgcgtac
     1621 agtttggata tagctttcct ccccgggaac gtcaattttg ttgcgaagat aacgaatata
     1681 tacgtccact acgtttgttt caacttctgt ttcgtatccc cacactttat ttagtaaaac
     1741 gtcacgtgct aataccacgt tcacgttttc cattaaagta agcagcaact cgtattctct
     1801 ttttgttagt tcaattactt catttccgcg acgtacgaca cggttttctt tttcgatagt
     1861 caaatcgcga taagtaatag tagtttgttt agccacattt ttatcgcctt caatatcgat
     1921 tcgacgcaac aatgcacgaa gacgtgctaa taattcttcg atagcaaatg gtttgacgat
     1981 gtagtcatct gctccgtggt caagtccaga tacacgatcg atcacagaat ctcttgcagt
     2041 catcataatg attggtgtat ttttgacttg gcgtacacgt ctgcagactt ctaaaccgtt
     2101 caattctggg agcatcagat ccaacaatat ggcatcccac tcattattta atgccgcatc
     2161 taaaccagtt cggccgttgt agtggacttc tgcgttatac ccttcatgct tcaactctag
     2221 ctcgacaaat cgtgccaagt tcttttcatc ttcaatgatt agaatattgc tcatttatta
     2281 ttttatcctt tctattctac tggcttttta aaaactctat cattataccg ataatatgct
     2341 taaaaatcta tagtcatttt atcatggata gcaattttaa aaaagagctg aagtctactc
     2401 ataacaatcg acttcagctc tttcgtcctc tttttatttt ctcatttcct aacagccgaa
     2461 agactattat tcttcatggt accatgagta atggaagatt ccttctttgt cgacacgctc
     2521 ataggtatgt gcgccaaagt aatcgcgttg ggcttggatt atatttgcag gcagacgttc
     2581 tgctctgtat gaatcgaagt aagcgatggc tgatgagaaa gtcggtacag gtacgccagc
     2641 ttggacagcc aaggcaacga cgtctctgac tgattgctga tattttttcg tgatttcaac
     2701 gaagtaatca tctagcaata aattttcgat ctcaggattt ttgtcatagg catccgtaat
     2761 tttttgcaag aatcgagctc ggatgataca tccagcgcgc cagatttttg cgatttcacc
     2821 aaatggcaag tcccaatcaa attcttttga agcgacacgt aattgagcaa atccttgggc
     2881 ataactcatg attttactga aatacaacgc ttctcggatt ttttcgacca attctttttt
     2941 gtctcccgtg aactcaaaat cgttcgtacg agacaaaatc ttgcttgctt gtacgcgttc
     3001 ttctttataa gcagaaatat agcgggcaaa tacagattca gtgatcaatg gaagtggcac
     3061 acctaagtca agcgcgcttt ggcttgtcca tttacctgtt cctttattcc cagctgcatc
     3121 aaggatcaca tctactactg gtttacctgt tccttcatcg tctttacgtg tcagaatgtc
     3181 agcagtgatt tctatcaaat agctgtctaa ttcgccttga ttccattctt taaagatatc
     3241 agccatttct tctactgaaa ggtttaaaat attcttcatt aagtcataag attcagcaat
     3301 cagttgcata tctccatact caataccgtt gtgtaccatt ttcacataat gtccggctcc
     3361 atttgggcca atatatgtga cacatggtgc tccgtcttct gcttttgcag aaatttgctc
     3421 taagattggt gcaactagcg cataggcttc tttttgtcca ccaggcatga ttgaaggtcc
     3481 ttttaaagct ccttcttcac caccggaaac gcctgtaccg ataaagttga tgccagagtt
     3541 ggctaactct tcattacgac gcatcgtatc tttaaagaat gtatttcctc cgtcaatcaa
     3601 aatatcgcct ttatcaaggt gaggaagtaa ctcttggatt gttgcatctg ttgccggtcc
     3661 tgctttgacc attagcaaga tccgacgtgg tttctcgata gattctacaa attcttcgat
     3721 tgtatatgtt gctttgaaat ttttatctgg gtgttcttct actacagcgg ttgttttagc
     3781 ccctgtacga ttgaataaag ccactgaata gccgcggctt tctatgttca aggctagatt
     3841 tttacccata acggccatac cgacaacgcc gatttgttgt tttgacatat gaactcctcc
     3901 tacatacggg atttctccct aatctcttac attatagctt aacacggcta cattgcaaac
     3961 taaagaacaa catatt
//
LOCUS       DAZULY010000223         3974 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000223 DAZULY010000000
VERSION     DAZULY010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.226"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..539)
                     /locus_tag="ACG81A_002439"
     CDS             complement(<1..539)
                     /locus_tag="ACG81A_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFS5015760.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQG"
     gene            674..1063
                     /locus_tag="ACG81A_002440"
     CDS             674..1063
                     /locus_tag="ACG81A_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015761.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1067..1831
                     /locus_tag="ACG81A_002441"
     CDS             1067..1831
                     /locus_tag="ACG81A_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5015762.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            1960..2907
                     /locus_tag="ACG81A_002442"
     CDS             1960..2907
                     /locus_tag="ACG81A_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5015763.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3037..3777
                     /locus_tag="ACG81A_002443"
     CDS             3037..3777
                     /locus_tag="ACG81A_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015764.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1319 a          683 c          807 g         1165 t
ORIGIN      
        1 tttccttgtc gttttctcac ttttttagtc cagaaaccac ctgaaatata ttttggctca
       61 taagaaatga taaaagcacg ctcttcgatt tctttgatca ggcgatacag ttcacgttcg
      121 ctttttctag gagaaagtat ttcgagaact agccgctcac cttccagccc cattgcagaa
      181 gtctgtgtca caccataacc gtgttctctc agtatacccg gcaaactttt atcttctgcg
      241 ctatttgctg gcaaaatcgt tgtgaccatg atgtatccta atgccaattt gtcttcaatc
      301 aaaattccga cactgatacc tactgcgtaa cctaacgcat agacaaataa attcagtgga
      361 ttatctaaac gatctaaaac aagggataag ccgacgatat aaatcgtgat ctcgaccata
      421 ctgacaagtg gagcaatcag tcgatagcct ttcattgtca acatgaaacg taacgtgttc
      481 agagtgatat aagcaaaatt aatgaggaag atcatcccca acatttttgg atcaatcatt
      541 tgtaaaactc ctttcattta tataacaaca atatcaaaaa aaatcgtaag tgaagaaaaa
      601 ttgaaacttt atacatacaa aaaaatgata aaatgattat aatagaatta taaagatagg
      661 ttaggtgatt aaaatgaaaa cggtcaatcg tgtgttttgt gccagccacg tgctaagtct
      721 ccttctcagt atatacttag gtgtctattc gattcaacag ccaattgagg tagagaacat
      781 tgtttttcag atgtcgctga cagatggttt aattttaagc gtttttttct ttttgatact
      841 gtttattggt aatattttcg gtagcgttgc cagttttttg aatttttcta tttatccact
      901 cctttcgcta gcattaggag tggtgggact ttgttctctg tgcttatttc ccgagccatt
      961 cagtcctgca tttatattat ttggtacact gaacctgttc cagacaactg ttggagcatg
     1021 gttgctatgg cgatcaggaa atttaatgaa aataggtgag taacaattga atccaattat
     1081 attgaaacgt tctgtccatc atattccagt gttagaagtt gttctgaaag aattaagaga
     1141 ggaacgtatt cccgttgtca tctattatca cggatggcag acaagtaagg agttagtttt
     1201 gacgcaagga agaaaaattg cgcagcaggg cattcgtgtg atcctgccag atgcgatgaa
     1261 tcatggggag cgcaaacagc cggtttcttc tattccatcc tatacatttt gggatagtat
     1321 ctacggaaat ctgtttgaat ttgacacact tatcaagcat ttagaaaaaa gaggtcttct
     1381 acatgatcag cttgcagttg gcggggtttc gatgggggga atgacaacat gcgccttgtt
     1441 ggctaaacat cctcatatag tagcgggcgt gtgtttgatg ggttcccctg cacctttgga
     1501 atatggaaaa gaaatcgtaa ggcgcgcatc ggaatataat tatgtcgtac caaaagacta
     1561 ttttgatttg atttcatggg ttaagagcta tgatttgtca ttgcagccgg aaaagttagc
     1621 tggacgtcct ctgttctttt ggcatggaca agctgacgaa aaaatccctt atatacaaac
     1681 ggaacgattt gtagaaaaaa atcggcataa gtcttacgga aaagaaattg tttttcgttc
     1741 ctcaaaaaca cagcgacata tggtacaaat accactaatg gaagaagctt ccactttttt
     1801 agcaaaaaat ttgataaaaa agtataaata attgaaacgg aaaatcactc atgacgatag
     1861 aggattttcc gttttttatt ttgggctata ataaactctg ttaggaaaat ttggtataat
     1921 tgatagtaag aatgacaaca tgttagaaag gaagagagca tgcccacttt agcagatgtt
     1981 gcaaaacgtg ccaatgtttc aaaaatgacg gtctctcgag tcatcaatca tccggaacag
     2041 gtgactgacg aattgaaaga gttagttttc tcagcaatgg ctgaactgga ttaccgtcca
     2101 aatattgctg caaaagcact tgtcacgaat cggtcgcaga tcatcaaatt atttatactg
     2161 gaagaaatcg acacaactga gccctattac atgaatttgc tgatgggaat cgctaaacgg
     2221 atcggtaaag cgcattattc tctccaatta gttacaaatg atgccttcga cgtcggttct
     2281 tgtgatggat acatcatcac aggtgtcaga aaaaaagact tcgattggat cgaccgtttg
     2341 gaaaaaccag ttgtcttgtt tggggaaaat tctgaaggat atgattatgt agacagcgat
     2401 aatgaatatg gtacagcaaa agcaacgcaa tatgccttag aaagagatta tcaaacgatt
     2461 atttatatcg gtatcgaagt ggacgagcct tttgaaattt caagggaaac tggttattta
     2521 tctgtgatga aggaagttga tcttcctagt aaaatcttgc ggtttccaaa tcattcgaca
     2581 gaagtagaaa gatatatgac agaaaactgg gcagatttta cagataaaac ttgttttgta
     2641 tgtagttcag atcggctagc actaggagca gtaagaggga ttataaatgc tggaggacac
     2701 ttgcccgaag aatttggtgt gattggattt gatggggtat ttttagacca aatcagttca
     2761 cctaaattga caacgatcaa gcaagatatt atacgtatgg gtgaagtatg tggagaaatg
     2821 ttgttaaaaa aaatagaaga aaaaggggcg agtcaaggtt atcgtcgttt ttttcctgaa
     2881 ctagttatca gagagacaac gaattaagtc ttattactat atatatattt atgaaattca
     2941 tcgctatttt cttaaaataa tcagcgcatt gctactgaaa acaatgaatc tgtgctatta
     3001 ttgagaaaag ttccattttt gagaaggatg gtttgaatga aagaaatcag acgaaaacat
     3061 ttccgttttc ctgcgtacga tgatgagtta ggcgtaaaat taaccaacga caagaagcga
     3121 gtattatttg acggacaaga agatcttttg actgaacaga atcagtcccc gtttgaacag
     3181 atgaaagaat ttcatttcac gaacacgaac accgagaaaa tccgtcgcaa gcgtcgtgca
     3241 acaaatggat tgaagtcagc tccgcgtaag gaaaatttgg aaaagcataa aggaaaatta
     3301 ccggattata ccggccatta ccaatcagaa atcacatcga caggacgaaa aaaacttttt
     3361 ggtgattcct ctcgttctgt cagacaaagt gaaacaagga cagagtccaa gcatactaca
     3421 cgaaacgcat ctgttgacac gaaggtgcgg gagcgttctt attttgttcc taaatatgta
     3481 cccgcatcga ttatcccaga ccctagagaa agcaaaattt ctgaggatca tttaatcgaa
     3541 tcgatgaaaa aaacggatga ctcttatctg ttgttcgata cagaaccgac gccgtatcaa
     3601 gaaaaaagaa atggtgagcc gtctgtacga acattcaatc gaacaaaaac gttagaggaa
     3661 acaaagcagt cagaagaaaa aaaatcgaaa aaaagccatg gtatcttaga acgctcatta
     3721 caaggattga tcgatgatca aggaaactct ttgggagaaa atagctattt taaataagtg
     3781 atatacaaat ttatattcgg ctacagttgg aaaggaaacg ctgcggcgtt tccttatttt
     3841 atactctatc cgaaagttta tatcgtatcc tcaaattttt ttgagtttaa gcgaattttt
     3901 tctcccaata agtaagaaaa taatggcaaa ataaaaaaac aaactatttt cgcagatcgt
     3961 ttgcaaaaat agtt
//
LOCUS       DAZULY010000224         3925 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000224 DAZULY010000000
VERSION     DAZULY010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.227"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(573..1130)
                     /locus_tag="ACG81A_002444"
     CDS             complement(573..1130)
                     /locus_tag="ACG81A_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015765.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="ACG81A_002445"
     CDS             complement(1309..1920)
                     /locus_tag="ACG81A_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015766.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="ACG81A_002446"
     CDS             complement(2033..2290)
                     /locus_tag="ACG81A_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015767.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="ACG81A_002447"
     CDS             complement(2887..3165)
                     /locus_tag="ACG81A_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015768.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="ACG81A_002448"
     CDS             complement(3199..3450)
                     /locus_tag="ACG81A_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015769.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          799 c          520 g         1411 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtat
//
LOCUS       DAZULY010000225         3881 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000225 DAZULY010000000
VERSION     DAZULY010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.228"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            71..1000
                     /gene="comGA"
                     /locus_tag="ACG81A_002449"
     CDS             71..1000
                     /gene="comGA"
                     /locus_tag="ACG81A_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFS5015770.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            957..2006
                     /gene="comGB"
                     /locus_tag="ACG81A_002450"
     CDS             957..2006
                     /gene="comGB"
                     /locus_tag="ACG81A_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFS5015771.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            2003..2281
                     /gene="comGC"
                     /locus_tag="ACG81A_002451"
     CDS             2003..2281
                     /gene="comGC"
                     /locus_tag="ACG81A_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFS5015772.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            2256..2726
                     /gene="comGD"
                     /locus_tag="ACG81A_002452"
     CDS             2256..2726
                     /gene="comGD"
                     /locus_tag="ACG81A_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFS5015773.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            2698..3045
                     /locus_tag="ACG81A_002453"
     CDS             2698..3045
                     /locus_tag="ACG81A_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015774.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            3008..3433
                     /gene="comGF"
                     /locus_tag="ACG81A_002454"
     CDS             3008..3433
                     /gene="comGF"
                     /locus_tag="ACG81A_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFS5015775.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            3405..3764
                     /gene="comGG"
                     /locus_tag="ACG81A_002455"
     CDS             3405..3764
                     /gene="comGG"
                     /locus_tag="ACG81A_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFS5015776.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
BASE COUNT         1250 a          636 c          813 g         1182 t
ORIGIN      
        1 tttttgtaaa ttcattttat ttgaaacaaa aagatcaaaa agacgtatgt tttttgtgga
       61 ggggagaata atggaaataa aacgattatc ggatcaaatg ataagtgaaa gttttgccaa
      121 acatgcccaa gacgtatata tattgccagc ttcagaagta ttttacatct attttcgcta
      181 tggaaaaaaa cgaacattct ggaagaaagt ctcttcggca gatggacagc aactgatctc
      241 aagatttaag tatttgggac agatggatat cggagaaaaa agaaaagctc agttaggtgc
      301 atttacttat tctttagaaa aacaggaagt tcgtctgcgg ttgtcttctg taggagatta
      361 ccttcaacga gagagtttag tgattcggct attatacggt acttttgaaa caccccatcg
      421 ttgctttagt tctcgagatt ttgcaactat tttacagaaa accgtcagac gtggattata
      481 ccttttcagc gggacagtcg gatcaggcaa gacttctcta atgtatcggt tggctttgga
      541 agaaaaattg caagtgatta ctatcgaaga tcctgtagag atagaagaac cgttttttct
      601 gcagctacag atcaatccga aaatagcaca gacttatgat acacttttga aactcgcatt
      661 acggcatcgt ccagacttgc taatcattgg cgagataaga gatgaatcaa ctgcacatgc
      721 tgcggtaaga gcagcactaa cagggcaccg ggtttttgca accgtacatg cacgcaatct
      781 tgaaggtaca ttggtcagaa tccatgaact gacaaagaaa aaagaagagc tatctgaatg
      841 tttagctggt gtcatttatc aagaactttt ggaagatgcc aatcaccatc cttctgtttt
      901 atggagttat caatttatgg aggaagaatc agtaaaaaaa tcatgggagg aaagttatga
      961 agaagctcag gaaaatattt ggcagtcaga actattctaa aggattaaca agaagccagc
     1021 aaaaccagtt tgcacaaacc atgggagaac tgcttttatc tggttttagt ctgcaagaat
     1081 ccctttattt attactcacg atccgttcgt ttccacctgt tattttggca aatatccaac
     1141 atcagctaaa ggaagggcaa gcgtttagtg aggtactttc gcaaatagga tatggagcag
     1201 agcaactggt acagatcgag ttagctgaac gtcatgggaa tctgccgaca actttgttgg
     1261 aaattgccaa acagtttcgg cttgttgaag gatttcgcaa tgatttgaaa aagctgcttg
     1321 catatccgat gctattgctc acctttttgc taggtatctt gtttagtatg cggttgttcg
     1381 ttttgcctca gcttattggt tcaggaatgg tcatgcaaga gcattggggt attcagctga
     1441 tacaagtctt tcattggtat gttctactat tcatcctagc ggttactcta ttggctttac
     1501 tagtaaggta tcgtctgatg aagttgtctc ctgttgatca gagtgaatgg ataagcagac
     1561 aattattgat tggaccgata tattccttgt atcaatcggc ttatttgtca ctagagattg
     1621 gaaagctttt tcatgaaggt ttcgaattaa ggcaaatcat agtctgctta aaggaaacga
     1681 aacaagaaag tctgattcag catcttgcct ctaagttgat tcaaggattg gaggcaggta
     1741 tgtcattggc tgagcagttc cagaattatc gttttctatc agaagaattc agtaaaattg
     1801 ttttacaggg agaggcaaag ggaagtttag gtaaagaact gattttttac agcgaatgga
     1861 caagaaaaca gcttttcaaa cgtcttcatc actggatgca ttttattcag ccaatcattt
     1921 ttttgggcgt agcggctttg attttattag tttatgccgc tgttttatta cctgtttacg
     1981 gaagcataga ggaggttttg ccatgaaaaa gagaaaaata tcttattata gcggttttac
     2041 attgatcgaa atgctaatcg tcttactgat catcagtgta ttagtgttgt tgttcgtccc
     2101 taatttgtcg cgttatagga atcatgtaga ccaagaaagt cgtgaagcga tcatccagtt
     2161 ggtagacaca caaaaagagc tctatgcgct tcaaaataat ggaagagttc ctactgtgga
     2221 ggaattgttg aatgaaggct atatcaaacg agaacatgca gaaatctatc agcggccttg
     2281 aaggtaaggt tttagcatca ggatatacgt tgtttgaatc actcttactg attttattac
     2341 ttacaacgat cctttctttt ccagttctcg cattttctgc atggaagaat gaattggcag
     2401 tctatcaatt tttcggccaa tttgaaaaac gaatctattc tacgcaaaaa atcgctattg
     2461 tgaatcagat ccaaacaggc ttttattgga atagtgaaga gaatcaaatc atttttcatg
     2521 ttccgaatcc agataaaata aattgggaag tattagatat tcctgatgag attaccttga
     2581 aacgccatgc ttctattact tttgctgcta gaaccgggaa tgaaagttca ctaaaagctt
     2641 atcaatttta ttgggaagaa aaagagcgga cgattacgta tcaattccag atagggagtg
     2701 gtcgctatac aaaaagaatt gaataaaaca ggatttgttt tgttggagtc ccttacagcg
     2761 cttatgatcg cttctgtctg tgtctactta gtgagctttg gccagctgtt tttgcttcag
     2821 gcagaaaaga aaagtgggga ggaactgcta tttattcgaa tgatttatga agatatcaag
     2881 acgcagcgga tgtatcacaa aagaccagag cgaagggaag aaatgatccc atacgatatc
     2941 caaattgtcg ataataatgg agtaatcgaa aaagtgtttg tcaatatgga gaaagaagcg
     3001 atcagtattg aaaaaacaag taattccagc atttacgctg attgaatgtc taattggact
     3061 gatgatattg agcagtctat tgctcacatt caatcttttg attcagcaaa ccatccaagt
     3121 aagtgaattt ttgcagcgta aagatcaaaa agagtggcta atatttttag cacaattaga
     3181 agaagaactc aagagcagtc acaatgtctc tgtgaaaaat cagcaactgc actacacgat
     3241 cgaggataaa cagtatatca tcgaaagata tcaggcaatg atacgtaaaa gaagaacttc
     3301 cggcggacat caacctatgt taacaagtat cactgagctt caattattgg aaaaagaaca
     3361 cacaatttct ttttatgtac actttgaaaa tggagaagat ggatatgcca tatggacaaa
     3421 aaacgatcag tagaaaatgt tataagggct ctattttatt ttctgtcttg cttttatttc
     3481 ttttatgtac gtttttattg atggcagcat tagaaagcta ccgtttgtca atggatctta
     3541 atatacgaat caaaaattat tatatagcaa aaataatgaa gcagatggct ttatcggaac
     3601 agtctacgct tgccgaaagt tcggaaggag ttttttacta cacaactgga tctgtaacat
     3661 atcagtgggt acaacagtcc ctattcttag aggtggaagt ctcaccgttt atctttcggt
     3721 tcacagaaga agtaaaaaat gatacagata ctagtacaga atgatggaat gttcgtgaaa
     3781 acgccctctt ttctgttata acactgtttt taataaaaaa atgtttgccg tttcacaaat
     3841 aaaaaacagt gaaaacgatt gaaattttgt caattgttcg a
//
LOCUS       DAZULY010000226         3876 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000226 DAZULY010000000
VERSION     DAZULY010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.229"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            158..2257
                     /locus_tag="ACG81A_002456"
     CDS             158..2257
                     /locus_tag="ACG81A_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFS5015777.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2453..3316
                     /locus_tag="ACG81A_002457"
     CDS             2453..3316
                     /locus_tag="ACG81A_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFS5015778.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3421..>3876
                     /locus_tag="ACG81A_002458"
     CDS             3421..>3876
                     /locus_tag="ACG81A_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS5015779.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT         1544 a          646 c          782 g          904 t
ORIGIN      
        1 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
       61 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
      121 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      181 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      241 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      301 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      361 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      421 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      481 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      541 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      601 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      661 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      721 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      781 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      841 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      901 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      961 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
     1021 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1081 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1141 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1201 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1261 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1321 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1381 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1441 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1501 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1561 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1621 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1681 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1741 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1801 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1861 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1921 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1981 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     2041 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2101 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2161 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2221 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2281 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2341 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2401 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2461 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2521 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2581 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2641 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2701 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2761 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2821 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2881 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2941 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     3001 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3061 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3121 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3181 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3241 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3301 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3361 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3421 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3481 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3541 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3601 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3661 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3721 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3781 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3841 catggaatct gtcaggaatg ctatgaaaaa gaaaaa
//
LOCUS       DAZULY010000227         3841 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000227 DAZULY010000000
VERSION     DAZULY010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.230"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..879
                     /locus_tag="ACG81A_002459"
     CDS             <1..879
                     /locus_tag="ACG81A_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFS5015780.1"
                     /translation="INEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGVSSAI
                     TTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIGHYTN
                     YAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTEDTLE
                     MLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYPDNLN
                     QYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCTLFGK
                     GFGNYDQDIKLKVYYS"
     gene            complement(952..1776)
                     /gene="nadE"
                     /locus_tag="ACG81A_002460"
     CDS             complement(952..1776)
                     /gene="nadE"
                     /locus_tag="ACG81A_002460"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFS5015781.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1788..3260)
                     /locus_tag="ACG81A_002461"
     CDS             complement(1788..3260)
                     /locus_tag="ACG81A_002461"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFS5015782.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT         1119 a          790 c          679 g         1253 t
ORIGIN      
        1 ataaacgaag aatccttacc ggtatatgaa gcattagcta gtaaaacaag gatcaagatc
       61 atccagctac tttccaaaaa aaagatgaat gtcaaagatt tggcaaaaga acttggagtg
      121 agcagtgcta tcacgacgat gcacgtgaaa aagctagagg aagcaaacat catcaaaaca
      181 gaaaaagtag gacaacaaaa aatttcgagc cttcgtgtcg ataaaatcga tattagtttt
      241 ccagaaaaaa tattcaatgc ttttgatacg aaagaaacat cgatacctat tggtcactat
      301 acaaattatg caattgaacc aacatgcggt ttagctacga tccatgattt tatcgggaaa
      361 gtggatgaac cacgctattt tatggatcct cgaaggatgg atgcccgcat tctctggttt
      421 accagcggat ttgtcgagta tcaggcacct aattttttaa atacagagga tacattggag
      481 atgttggaag tttctgtgga aatttcttct gaatttcctt tttcaaatga caattggcct
      541 tcagatatca ctttcagcct aaatggtgtg gagctaggaa catggacgag ccctggagat
      601 tttgctgata tccgcggtaa atatacgcct gattggtatc cagataatct taatcaatac
      661 ggcttgctga agacgatccg aattaccaaa cacttgacga atatgaatgg agaacccttg
      721 tcaaacatca cgatcaacga catccctaaa gagcaagaca cttggcattt gcgaatcgaa
      781 gtgaaagacg atgccaaaca tgtggggggc tgtactttat ttggtaaagg attcggtaat
      841 tacgatcagg atatcaaatt aaaagtttac tacagctgat atttttcttt tctattttat
      901 aaaacaaaaa cagccaatcc ctatgaaggg acggctgttt ttgctttatt gttatttcca
      961 gaaatcatcc aaaacggaaa ttggcaaatg tcgtttatgt tgtgtcttgt tccaccatgc
     1021 ttcgattttt tcttgtgctt gagcgggaac ttcttttcct tctagataat catcaatttc
     1081 attatatgtg acacctagtg cgacttcatc tgcaatcatt ggtttcccat cttcaagatc
     1141 tgcagtcgga atttttgtgt ataatttttc cggtgcattc aatgcttgaa gcaattgttt
     1201 cccttgtcgt ttgtccaaac ggaataatgg caaaatgtcg gctccaccat caccaaattt
     1261 cgtgaagaat ccagtaatgt tttctgcagc atgatctgtc cctaatacgg ctcctgctct
     1321 ttctccagct acggcatatt gcgtaatcat acgttgacgt gctttgatat ttcctttgtt
     1381 aaaatcgctg attcccacac ctgcttcgct taatacttgt acttgtgcat caacagctgg
     1441 tttgatattt actcgcagac taacatcggg ctggataaag tcaagagcag cttttgcatc
     1501 ttcttcatct gcttgttcac cataaggaag acgaacagcg ataaattgat agttttcatc
     1561 ttttgtttct tcacgcattt cttccatcgt tagttgcgcc aaacggcctg ctaaagtaga
     1621 atcttggccg ccgctgatac ctagtacaaa agttttaaga aacggatatt ttttcaaata
     1681 tgctttcata aagtcaatac ttttacgtat ttcttctttt ggatcaattg ttggtttgac
     1741 acctaattca ttgataattt ctgtctgcag gctcatttat tctcctccta tgcttctgta
     1801 tgaagttctg ctacgctttt tttcattcgt tccatgatag ccattttatg gttccagcat
     1861 tcagtagaca gatcgactgg gtatttttgc ggattcaagt ctcgtttata ctcttcccat
     1921 agtgaatcta gattttcttt tgtatattct ttgatttcgt ttagtgcagg taattcgtaa
     1981 actctttttc cttcgataaa gatatcttgt aaaactgggc gagctgtaaa gtctcgaaca
     2041 gttttattga taaacgtatg cactggatgg aacatgaaga tagcttcttc ttctcgaggg
     2101 tcttcatccc ataatgtcac gtagtctcct tcagatttcc catcaaaatt tcgagtgatg
     2161 cgccacactt gttttttccc tggagtagtt actttttctg cattgcttga taacttgatc
     2221 gtatccatca tttttccttc atcatcttcg atcgatacta gcttgaatac tgcaccaagg
     2281 gctggctgat cataggctgt gatcaatttt gtcccaacac cccatacatc aattttagct
     2341 ttttgcatct ttaaattcaa aatcgttgct tcatccaagt cattcgaagc atagattttt
     2401 gcttccgtaa atccggctgc atctaattgt tcccgaacac gcttagatat ataggccata
     2461 tctccactgt cgatgcgtac gccttggaag ttgattttgt cacctaattc tttcgccacg
     2521 cgaatcgcac ttggcacacc tgatttcaac gtatcataag tgtcgactaa gaacacgcag
     2581 tctttatggg ttttagcgta ggccataaat gcttcataat catttccata agattggact
     2641 aacgaatgag catgggtccc gctagctggt atcccaaata tcttcccagc acgtacattg
     2701 cttgttgcat ctgctccgcc gatatatgcc gctctagttc cccagactgc tgcatccagt
     2761 tcttgtgccc gtctagttcc aaattccaat agtggatctt ctccgatgac agatttgata
     2821 cgagctgctt ttgtcgcgat caacgtttgg aaattgacca tattcaacag tgctgtttct
     2881 actaattggc agtgcgctaa aggcccttcc acttggatca atggttcatt tgcaaaaact
     2941 agttctcctt cacgagcaga acgaactgtt gctttaaatt caaaattttg taaatactcc
     3001 aaaaaacctt caggatagac ttctaatcca cgcaagtaag caatatcgga atcattgaac
     3061 gtcagattct ctaaataatt gactaaccgt tccaatcctg caaaaaccgc gtatccgtgg
     3121 ttaaaaggca tttctcgaaa gtaacattca aaaacagcat gtaaatcagc tcgacctaat
     3181 tcccagtatg tctgcatcat attgatttga tatagatccg tatgcagcgt caaactgtcg
     3241 tctgggtaag tattattcat cttgtattac tcctcttggc aagttttcat ttcctaccca
     3301 ttataacaaa aaaaattaat aggaaaagaa atcttccatt ttttctgaaa aagcacatgt
     3361 tttacggata tttcttgcta aaaatgaact ccatctgcta aaactatcct ttcttattgg
     3421 tatttaacct aaacgagaaa gaatacttat tttattcaat gtatccgaga cacgaagaaa
     3481 ttagcgtttt ctatttttct tacttgataa aataagtaga ttttcctact acaatcgtag
     3541 ccgttaagat accgatgatt gaacaaatat aaccaaatgt ctctacaaca taatcaatgg
     3601 caaaagtttt tgaagcaata atacgtcttt ccttttttgc ttcttatccc caaagaaacg
     3661 gatataactt attatgtatt gaattttttt ggaaaggcga attatgacaa gatatacctt
     3721 gcgttgaaaa aactcgcgga aaaaatatca cacgttttta aaaagataca aagtaagaaa
     3781 gcagctgtca atagaatgga aacaaaaatg gcgagacatc aatcagtctc gccttgtttt
     3841 t
//
LOCUS       DAZULY010000228         3831 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000228 DAZULY010000000
VERSION     DAZULY010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.231"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            135..1085
                     /gene="yidC"
                     /locus_tag="ACG81A_002462"
     CDS             135..1085
                     /gene="yidC"
                     /locus_tag="ACG81A_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFS5015783.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(1131..1532)
                     /locus_tag="ACG81A_002463"
     CDS             complement(1131..1532)
                     /locus_tag="ACG81A_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5015784.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(1546..2355)
                     /locus_tag="ACG81A_002464"
     CDS             complement(1546..2355)
                     /locus_tag="ACG81A_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5015785.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(2342..3232)
                     /locus_tag="ACG81A_002465"
     CDS             complement(2342..3232)
                     /locus_tag="ACG81A_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5015786.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(3245..3724)
                     /locus_tag="ACG81A_002466"
                     /pseudo
     CDS             complement(3245..3724)
                     /locus_tag="ACG81A_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
BASE COUNT         1201 a          818 c          647 g         1165 t
ORIGIN      
        1 tgtctttttt tcaaaatttt tcgctacgta aaagaacgaa tatttatatg aaatttctat
       61 gaagttgatt gaattccatg cgctttttta gtatagttaa gtttgtgtaa aaattataga
      121 agaggaagta ttctatgaat aaatggaaaa actggttatt gggatccgga cttgtcagta
      181 tcctgctctt tttatctggc tgtgtgcgga tggattcaaa cggaaaccct gacacttcgg
      241 gaatcgttta tcgagtactc gtccatccta tgggccaagc gattacatat ctagttgaga
      301 atttcaattg gtcttatggc tgggcagtta ttgcaatgac tgtgatcgtc cgtatcatca
      361 tcttgccact aggtattagt caatcgaaaa aaacaatgat ccaatctgaa aaaatgcaag
      421 cgttaaaacc acaagttgaa gcagcccaac aaaaattgaa agctgcaaca acacgtgaag
      481 aacaaatggc agcacaagca gaaatgcagc aagtataccg cgaaaacgga ttgagtatga
      541 ccggcggaat cggctgtctg cctctattga tccaaatgcc gatcttctct gccttgtact
      601 ttacggcacg ttatacagaa ggtattcggg aatcatcttt ctacggcatc gatttaggct
      661 caccaagtat ggtacttgtt gtcattgcag gggtcgccta tctgcttcaa ggatacatct
      721 caacgatcgg tattcctgaa gaacagaaaa agacgatgcg tacgatgcta atcgtctctc
      781 ctgctatgat cgtcttcatg tcgatcagtg cgccagccgg tgttacactt tactgggtag
      841 tcggtggtgt gttcagctgt ctgcaaacat tcatcacaaa cgttatgatg aaaccacgtt
      901 tgaaagcaca agtggcagaa gagttgaaac aaaacccgcc aaaacaagtc gtcactccaa
      961 gaaaagacgt gacagaatca gcaaagccag ctaacaacaa acaagcaaca aaacaaatca
     1021 aacaaccaaa aaacagtaca aacaacaaag gccgcaacgc aggaaagcag cagaagcgga
     1081 aataaaaaca taggacaaaa aagatgagag ctaagccctc atcttttttg ttactcttcc
     1141 tcttctgcta ttccattaat taattcgttg acagagacaa tgctttcaca tcctgcacga
     1201 acaaactcgg gctctgaacc aatcatttgt gagttgataa attcaatgac catcggaaga
     1261 ttcatcccag cataaagagt aatctgttgt ccttccatta ttagtttaga aatgatattt
     1321 gcaggtgtac cacctaaaag atcgcatagt accacaaaat catttaacga atctgtgaca
     1381 gtgagaaact tttgtttgta tgtttcagtg ccatcttctg gtaataaagg taacgtgtag
     1441 atatcctctt gaggccccat gatcatttct gtactttttt tcaattcctc acaaaatttt
     1501 ccgtgactaa tcaataccaa ttgtctactc atttttcgct ctcccttatt tcgaaatcac
     1561 acccaatgca gataaagcaa cacacacaat taatacgata aaaatcgctc gagtaggagt
     1621 catgtttttc tttcctaata accaataaac accaccgacg atcccagcag gtaaaatttt
     1681 aggtaagatc atatcgatat agttttgcat attcaatgtc acatcgccaa tcgttggtgc
     1741 ccatgaaaat ttaacattga tcattgtcgc aactaatgcc cctaccatga atacccctaa
     1801 taaagtggct gcgtccgtta atgcagttaa gcgagattgc atagtagtaa ctaaggaaat
     1861 tccttctttg tatgcaaatt caagttgctt ccaacgaaaa accatgacaa cgatctgagc
     1921 aaaaatccat ataaacacac ctatcggatt tccatcagtt gccatattcg ccgccaaagc
     1981 cccaaaaatc gttggaatca aagcagcaaa tacggaatct ccaatcgctg caaaggaacc
     2041 catcaatcca gctttcagcc ccgatacggt ttcctttgaa gcaattccct ctttttcttc
     2101 cattgcaaga tcaatcccag taatgattgt attaaaaaaa ttagaagtat taaaaaattg
     2161 cgtatgtgtt ttcatcatct cttttaattc tggtgtattg tctccatata tttttcttag
     2221 ttgtggcaag atagtatata aatagccaga accctgcatt ctctcatagt tccaacctaa
     2281 ttgaaaagtg aacaaacttc ttcgattgat ttgcttaaaa tcttcttttg ttaatttgta
     2341 attagatttc gtcatcttcg atttccccct caacagaatg tgatggctca ctagctaatg
     2401 gttgatggtc tttgttttga tcattcttat aactgatcat tgctagtgct agaccaatca
     2461 atgctacagc aagcatcggc agcgaattga aagttgccgg aagatcttta acaacgcttg
     2521 ccacacttgt tcctaataat tgaacattag agaataacgt tgctaataat gccgtgatcg
     2581 taaatcctaa aataagataa gatagatgtt tttttactgg taaatagcgc agaagaatgg
     2641 caaacccaac agctggtaaa acagctcctg ctactgaaag tccatcgcct agccatttta
     2701 gattcccgtt taaaacagaa acaacatttt caactaatcc accaccaaat gctaaagcta
     2761 aaaatacagg aatcatacga gagagcgacc aaggtaaagc tcccattaag aaattgcgtt
     2821 cgattgcttg ataattcatg tcttctacgt gacggtcaat acgatgggca aagtatgtgt
     2881 tagcgaatct agctaaaata tccaattgga tcattaagct tgctacaggc actgcaatag
     2941 cagcaatcgc ttgttctgga ttcattccaa gagaaatcga gaaggcagtt gctaaaatcg
     3001 tcccagagtt ggcatctatt tttgatgcgc ctccaaacgt ccccactcct aaaactgtca
     3061 actgcatact gccaccaata atcaaacctg cgtttaaatc acccattact aaacctgcat
     3121 ataaccctgc aaacactggc gcactcattg aagaatagat ctgtaattca tctaaaattt
     3181 gataacctgc atataatgtt aataataaga tctgccacca ttgaatatcc atggatcatt
     3241 cctcctattt gattaaaaga ttcataaagt cttctgcttt atcgtttggt accatttgag
     3301 caattaaatg tataccaagc tgatctaatt ttttaaatac ttcgatatct gcatctacca
     3361 cattgattga ttttgtaatg gaccgtgttt cattcgtttg tgacatattc cccacattta
     3421 cttctttgat aggaactcct ttttctacta aagataagat tcgatctggc ttttttgcta
     3481 cgatcaatac tcgttgagag tcatatttgc cagctaaaat atttgttgct gctttttcga
     3541 ttggcaaaat gcttaatttt acccctgctg gagtcgctaa cttcaatcca cttttttcaa
     3601 ttgtgtttgt ggctacctca tcatccacga ccatgattcg actaatattc agtttcgttg
     3661 tccaaagatt tgctacttat ccatgtatca aacgtccatc gattcttaca ccaataattg
     3721 ccatcatgca ttctccttat ctattttttc tatttattgt tcaattaaaa ctgtgacatc
     3781 atcaaatatc ccttctgttt caaagataac gttttcattt tttctttctt a
//
LOCUS       DAZULY010000229         3827 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000229 DAZULY010000000
VERSION     DAZULY010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.232"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..399)
                     /locus_tag="ACG81A_002467"
     CDS             complement(<1..399)
                     /locus_tag="ACG81A_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFS5015787.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLS"
     gene            complement(515..1429)
                     /locus_tag="ACG81A_002468"
     CDS             complement(515..1429)
                     /locus_tag="ACG81A_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFS5015788.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(1520..1948)
                     /locus_tag="ACG81A_002469"
     CDS             complement(1520..1948)
                     /locus_tag="ACG81A_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFS5015789.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(1953..2327)
                     /locus_tag="ACG81A_002470"
     CDS             complement(1953..2327)
                     /locus_tag="ACG81A_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5015790.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            2613..2798
                     /locus_tag="ACG81A_002471"
     CDS             2613..2798
                     /locus_tag="ACG81A_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5015791.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            2810..3058
                     /locus_tag="ACG81A_002472"
     CDS             2810..3058
                     /locus_tag="ACG81A_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015792.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            3089..3268
                     /locus_tag="ACG81A_002473"
     CDS             3089..3268
                     /locus_tag="ACG81A_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015793.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(3311..>3827)
                     /locus_tag="ACG81A_002474"
     CDS             complement(3311..>3827)
                     /locus_tag="ACG81A_002474"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015794.1"
                     /translation="VVFILNFFHLTIFQWMSIISFILSTFLTGIKIKGMRPQLEVELN
                     ASYFAADMIYTKVIISNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNR
                     IYSEAVPLNIPPKSAISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTN
                     FPIEKLVKELN"
BASE COUNT         1203 a          614 c          638 g         1372 t
ORIGIN      
        1 tgacaagtca tctttatata tcatatcttc aaaaatatta tttaaatcta ctgtattaat
       61 accagaaacg atgtaagtct caggtatctt tttctttata gtgtttaaga tattattcgt
      121 aggataatat gtagactcct taacttgtct tttgtaaata actaaccatt cttcgacaag
      181 ctcatgaaat gttagatcag gcttctgaag tgttttttcg tttaatttta tatcaatttt
      241 tttatttaac tctagcaatg ccactttctg agtttctcga gatttatttt tataagtaat
      301 gctcactttt tttctttttc tagtttttgg atcaatatat cgctcattat atttatatac
      361 tttttttccg tttttatcag ttttggtttc aatccacatt gttttcatct cctatttgtt
      421 gctataatag gaatggataa gtaagccaat tatagcaggt ttatttttca tcacgtccac
      481 aaactttggc gagggagggg gcgtgttttt ttgtttagta gctacctttc catgaagtat
      541 ctgcgaagaa cacttcaaat gttgttccat catttttcac ttcaaaatat gctgttcctt
      601 gtgcggattt acccggttgg atggtttccg aaaagtaatc tctactagct aaatcagatt
      661 taatatctga tgaatcataa aattctaaat aacttgaact tgcatcaaaa ggtgtagtcc
      721 ctgtgttttc tacaagaaaa tctacctttg cgtaaaatag gccttctggt ttatagaaat
      781 catcaccgta atcttttgat acagactgga cagttacgtt aatagagtta ccattttcag
      841 aagtaaaagg aactgcttct cctatactat gtgcaactga tgccgtctgg ggttctgaag
      901 acgaagttgt agttgtagtt gtagtagagc ttgaatcttt ggattttcca tctttatcta
      961 agaaagttcg ttgattataa tatacaacct gttcagtttt tggactaacc caaattaaca
     1021 ttgtttcatc atcagaatca gttgaataag tatattggag catctctaat ttttttatat
     1081 cttttgcact atttccatgt tcaattaatt cagagtgctc tttataataa tctccttgtg
     1141 catcctttga aatagataca attaattcat cccgtgtgat agcattgtct aattctttgt
     1201 aaacaaaatc atttgtccat ttacgtaacg gtgaaccaag tgtctgataa acttgagtta
     1261 aatcatcacc aacttttatt ttatgaacgt ctgatgaagt tagttttttt gtttcatatt
     1321 cagatgttga tgaaacagta ttttctttag tgctagcaga attaggacta gtggagcatc
     1381 cgcttaatac caagcctaaa aaaagcaacc caaaaacaat ttttttcatt tttaattcct
     1441 cacttctatg atatgatttt tttgtaggat cttagaaata aggtttttag tccgtgttgc
     1501 agcacggact ttttttacat tatataagaa agcgataagc gctttctgga agtccgtaaa
     1561 gattctttaa ttcctcaatt cgtttaggat attgatcatt gtcttctttg tatagagaaa
     1621 caatgagatt agcagcaaag caattagctt cgctttcaga tttgcttcta gatgttcttg
     1681 ttgatacata gtaactggat aagccacgat gaaaaatagc gtgacctaat tcgtgagcgc
     1741 aaatgtagaa tctttcctca gagtctctca gttcatcatt taagaagatt attgcacgac
     1801 ctctaatttc ttgaaactgt cctttgggat tctcgataaa aggaacgtat tgaattttaa
     1861 tgcccatctt ttcacaaata taaaaaggat tagcggactg gtatttccgc ttcaactcct
     1921 cgactaaatt aatcgtatcc atctccataa gctcacatct ttttgccttt ttctttgtct
     1981 tctttcacaa tatcccagaa ggttccaata aggatatctt ttacgcgttg gacctgttca
     2041 ggcgtcagtg tttctccgcc ataagacata ttgacatttg aatctagtaa tttatcaagt
     2101 tcaactactt catcttttgt tgcccagtca ggaacttggt ttctaccgag taaataatca
     2161 gtagttacat taaaataatc tgctacaagt tgtagtcttt tagtactagg agttctattt
     2221 ttccattgat aaatcgtgtt tttaggtata tttaattctt cttccaattg agttatactt
     2281 atgcctcttt tatgagctaa ttcctttatt ctatctagta aattcatttt cgcacctcac
     2341 aagctacgaa aacactaata aaaaaattag ttaaaggtgt tgacaactaa taaaagtgtt
     2401 agtatactgt tttcgtaagc taaaatatta gctaaaaaga cttgataact aataaacact
     2461 tcacggtcgg caaactctga aatgtaaatt actaggcatt tctgtgtctt atttagctat
     2521 gtctatatac taataaaaat attagttatt gtcaacgaat attagctaat tttttagctt
     2581 acagattatt tttttagaaa ggagctattt ttatgtctga gaatttagac ttaaaaattc
     2641 gagcggagat gagaaaaaga agaatgactt tcaaagaact agctgcgctt atcggtattt
     2701 caggagctta tttatcagat attctaaacg gcaatcgtga tggaaagaaa gcacaacagc
     2761 atatcgaaac agtgaaaaaa atattggaca tccgataggg gggtaggcga tgacaaaact
     2821 aaaaaaacaa gattttgtaa aaaaatacaa ttattctcca tctacttatc aacgtcgaat
     2881 gtcggaacta aaaaatacag caattttctc agcggcgtat gaacgggtca caggacaaga
     2941 agtttggatc aatacagaat tatacgataa atttttgtct ttcaaatcct ataacaggtt
     3001 acgcacaaga aaggtaacac ctaaagaatt tatcgagaag catttagttg atttataaaa
     3061 aacagaacat tttgagaggt gaaggttaat gggtaaattc aacagagcat tggtattcag
     3121 cgcaccgcta atcatctacg ctttaggact ttggggaagc aggcaagcgt tgataggaac
     3181 gattgtttac atggtttgga tttttatggg gcttgatgaa gctgaagctg agtacagagc
     3241 gaaaaagcca accgaggggg ctgactaaaa atgaatagaa aagagaaact agaatgtata
     3301 ttactattac tcagtttaat tctttcacta atttctctat tgggaagttt gtatttttga
     3361 tatcaacaat tttgtggatt tgggttctat ttagagtaaa tatcattttt gtttggtgat
     3421 taaggagttt attaaaatta attttcccta cttcaaatgc aaggtaacaa gatatagcac
     3481 ttttaggagg aatatttagc ggtacagctt cagagtatat tctattatca tcgactgatc
     3541 ctcctttagc aattaatttc ttaaatggag tagctgacca tctgcggttt aagttagcgg
     3601 atgaaagttc taaattaact aacatagctg gttctgttga atagttagaa atgattactt
     3661 ttgtataaat catatctgca gcgaagtaag aagcattaag ttcaacttct agttgagggc
     3721 gcattccttt tatttttatc cctgttaaaa acgtactcaa tataaaactt attattgaca
     3781 tccattgaaa aattgttaag tgaaaaaaat tcaaaataaa caccacc
//
LOCUS       DAZULY010000230         3718 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000230 DAZULY010000000
VERSION     DAZULY010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.233"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..714
                     /locus_tag="ACG81A_002475"
     CDS             <1..714
                     /locus_tag="ACG81A_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5015795.1"
                     /translation="QAFLITKNTILYNVAFILLNLLISVVFAIIMSELRNKRLVKVYQ
                     TMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIG
                     TWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAV
                     GNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFV
                     LLMITNTIARRFDSDSALF"
     gene            726..1646
                     /locus_tag="ACG81A_002476"
     CDS             726..1646
                     /locus_tag="ACG81A_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS5015796.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1687..3144
                     /locus_tag="ACG81A_002477"
     CDS             1687..3144
                     /locus_tag="ACG81A_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS5015797.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3489..>3718
                     /locus_tag="ACG81A_002478"
     CDS             3489..>3718
                     /locus_tag="ACG81A_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFS5015798.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLL"
BASE COUNT         1200 a          640 c          750 g         1128 t
ORIGIN      
        1 caagcatttc tcatcactaa aaatacaatt ttatataatg tagcatttat ccttttgaat
       61 ctgctgatct cagtcgtatt tgcaatcatt atgagcgagt tgagaaataa acgactcgtt
      121 aaagtttatc aaacgatgtc cttactacct tattttctat cttgggtcat tatcagttac
      181 tttgtttacg cttttttaag tcctgacaaa ggaatattca atcagtggat cactggacat
      241 ggaggagaag ctgtcaattg gtataatgaa ccgaaatatt ggccgtttat cttggtgttc
      301 attggtacat ggaaagggat tggttataac agcattattt actttgcatc tgttatggga
      361 atcgatccga cttattatga agctgctatg gttgatggag ctagcaaatg gcagcagatc
      421 aaacatgtga cgataccaca acttattcca ctaatgacga ttttaacgat tttagccgta
      481 ggaaacattt tccgagctga ttttggcctg ttttataaca ttcctaggaa ttccggtgca
      541 ttatatgatg tgactcaagt tttggatacg tatatctaca atggcctgac atccacagga
      601 gattttggta tgacggctgc tgcaggactt taccaatcag ttgtcggctt tgtcttgttg
      661 atgatcacaa atacgatcgc ccgacgtttt gatagcgatt ctgcattatt ctaaggagga
      721 aaaaaatgaa aaaaaagaaa gtggagagag tccaagtccg ctctttcaat aagacggcta
      781 attttttctc taatataatg attgcactct ttgctatttc ctgtattttg ccatttatat
      841 ttgttatcgc catctctttt accgatgagt ccatattgtc gactcacggg tacagttttt
      901 ggccagaaaa gttcagtata tttggttata cgtacttgtt cgaccagatg caggataaaa
      961 ttttccaagc gcttttcgtc actgtacttg ttactgtttt aggtacactg atcaatagca
     1021 cggctacttc tttatatgct tatgcgattt cacggtcaaa ctttccattc cgtcgttttt
     1081 ttaccgtttt ttgtttgatt acgatgttgt tctcaccagg gatggtggcc aattatcttg
     1141 ttatgacaaa tcttcttcag ttaaaagata cgatttgggc attgatcttg ccaatggctg
     1201 tcagtccctt caatattatt gtgatgcgca cattctttaa acgttcggtc tctgattcga
     1261 ttattgaatc ggcacggatc gatggagcta gcgaactgcg aatatttgtt cagatcgttt
     1321 taccgttagc tgttccaggt attgcgacaa tcagtttatt tgccgcattg ggttactgga
     1381 atgactggtt caatgcttta ctatacatcc aagatgataa gctagttcca ctacagtatc
     1441 ttttgatgaa aatccaaagt aatattcaat atctgacaca aaattcagga gcaggaagcc
     1501 aattgacagg cggattagcc tcagttccag gagaatctgc cagaatggcg atcgtagtca
     1561 tctcgacgtt gcctattgcg atcagctatc catttttcca gaagcatttt gtcaaaggtc
     1621 taacaatcgg cggagtaaaa gaataaagaa acaaaaatat aaatcataaa attaggagga
     1681 agaaaaatga agttatggaa gaaactagcc tttacaggtg tttcagcgtt gttgttggga
     1741 acattagcag catgcggagg ttctggttct aaagaccagg cagaagctag caattcggac
     1801 actttgcaga tgtatcaaat cggagataaa ccagataatt tcgatcaatt gatggaagta
     1861 gcaaacaaac gaatcgaaaa agagatcggt gtaaaagtca atatcaatta cattggctgg
     1921 ggcgattacg aaaagaaaat gaacgtcatc atttcttccg gggaaaatta tgatatcgcc
     1981 tttgcgaaca actatgtttc caatgcccaa aaaggagcct ttgctgattt gactgaatta
     2041 gcgcctaagt acgctgaaaa agcttataat gatttagatg aagcatatat taaaggaaat
     2101 ctggtcaatg gaaaattata tgctttccca gtcaatggaa atgtctttgc tcaacaagta
     2161 cttactttta acaaagcttt actagataaa tacgatcttt caattgatgg aatccaatct
     2221 tacgctgatg cagaaaaagt cttgaaggaa ttccatgaaa aagaaccaaa cacggcagct
     2281 tttgccattg gacaaggctt caaagtccaa gggaactttg attatccttt agggaatacg
     2341 ttaccatttg ctattgatct ggatggagat gattcgaaaa tcatcaacca atacgataat
     2401 gaaacgtata tcggactgtt acgtacaatg catagctggt acaaacaagg actcattcca
     2461 agtgacgctg caacgagcaa taaggattat ccactagaag gaaatacttg gctaatgcgt
     2521 ggagaaacac aaggaccgta tgattatggc gatacaattt tgacaaatgc agcaaaacaa
     2581 gaacttgtct caaaagcaat tactgttcca ctaaaatcta cagctcaagc acaaatggca
     2641 aattttgtcg tatcaaatac ttcaaaaaat aaagaaaaat cagtagaact tctagggctg
     2701 cttaacagtg atccagaatt attgaatggg cttgtttggg ggatcgaggg agaagcatgg
     2761 gaaaaagtcg atggttcaga tgataaaatc aaattgctag atggttatca gccaaataca
     2821 catatgtctg cttggaatac aggaaacaac aagattctat atacacaaga gtctattacg
     2881 gatgacatga ttgccaaacg tgatcaatcg attgctgatg cagaaacttc accgatttta
     2941 ggattcagct tcaacactga cagtgtcaaa acagaattaa gtaatatttc taatgtcatg
     3001 aatcaatatt tagatggatt gaatacaggg actgttgatc cagatgaaac attaccgaaa
     3061 ttgaaagacg ctttggacaa ggctggctat gataaggtat tgaaagaaat gcaaaaacaa
     3121 tatgatgagt tccgacaaga ataataatga ataaagacaa aggggttgtg acaaaagacg
     3181 tgtcacaacc tcttgttccg aataaacggt gttcaaaagc cagtttctcg gtattaagct
     3241 aaatatatgt aaaaatgtgg aaagccatat tcacaaaatt tttcttacta ctcgaaacta
     3301 agcggctatt tcacaaactc ttggtaaacg gtgttcaatc agctaaccct cggtattcgc
     3361 tcacaaaacg acaaaatatg aggaactatt tttccgtttg tttctctaat aatcaggtca
     3421 aggtgctgat tgcccaacct ttttttgtat aatgagaaag tataaactta atgtttggag
     3481 gagaagatat gttagggaag gttttggaag agttatttat ccggatctgg gtcattataa
     3541 aactaacctt gtatttctgg atatatacat ttgccggcgg gatcatcttt ggattaggtg
     3601 cggcatggaa aacagtcaat gaactatttt atttgtacgg attcgagtat aaagaaatca
     3661 caataaaaag aggctggaat atatataaaa gaaatttttt aagaggcaat ctgctatt
//
LOCUS       DAZULY010000231         3668 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000231 DAZULY010000000
VERSION     DAZULY010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.234"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..300)
                     /locus_tag="ACG81A_002479"
     CDS             complement(136..300)
                     /locus_tag="ACG81A_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015799.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            595..1923
                     /locus_tag="ACG81A_002480"
     CDS             595..1923
                     /locus_tag="ACG81A_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFS5015800.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2125..2958
                     /locus_tag="ACG81A_002481"
     CDS             2125..2958
                     /locus_tag="ACG81A_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFS5015801.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2974..>3668
                     /locus_tag="ACG81A_002482"
     CDS             2974..>3668
                     /locus_tag="ACG81A_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFS5015802.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQ"
BASE COUNT         1220 a          580 c          762 g         1106 t
ORIGIN      
        1 aataaagctt atgcaaagaa aaagaaatcc aatcacttgg gtttcttttt cttttattat
       61 tatgatgaat caagctttct gtttttgtca cgtatgttgt aatctttacg aaagagtaga
      121 gttggctact cataatcaac catacttccc atcatcgcta aatacgaaat aaatcagcat
      181 tcctacacca atcaaagcac aagctgcacc aagaaaaggg atgacattcc caaagttatc
      241 tgtattcata agtatgccac cacccacaaa caaaaagccg ccagcaatca ggtttttcat
      301 tttattcatc tcctttctta cgttatagta tttccttctt tcaatgagta ttttgaaagc
      361 tatcttattg tatgatgttc ttctaggcct cttctttatt atacaggcca ttttcgttat
      421 ctcatagaaa tattcctttt tatctgaaaa ctattgtata ccgtatgaca tttttcttca
      481 tgaagttgta agtatattct cattcaaatg aaaatttcat catagaagag attgtatttc
      541 taaattctta agagtaaaat acgaagtata acttggattt ggaaggatga ttggatgaaa
      601 gctattacgc ctgatatgac aacaaaattt aacaaacagt ttcaagcaaa tatcaaacag
      661 caggcattgc agcgggcagt tgtgaagaac ggaattactg cttctgctga aaatgtttca
      721 gcacatgtgg agaatacacc ggtattttct atagatttgg ctacggggaa agtggctaat
      781 caaatgcaat cggggcgttg ctggatgttt gctgcactga acacattccg tcataagatg
      841 ctgaatcaat tccatttaaa agagtttgaa ttatcacaaa attacacttt tttctgggat
      901 aaatatgaaa aggcaaatta tttctatgaa aacatcattg caacaggaaa agaggcaatc
      961 gatagccgga aagtagcttt cttactcgct actcctcaac aagatggcgg ccaatgggat
     1021 atgatcgttt cattattcca aaaatatggc gttgtgccta aatcggtgat gccggaaagt
     1081 gcgaatagtt caaattctcg agatttgaat aactacttga acaaattact gcgtaaagat
     1141 gcagtagtgt tacggaaaat ggttgctcaa ggagaaacat tagacgctat tgaagaaaag
     1201 aaagaagaaa tgctagaaag catttataac ttcttagcta tctcattagg aacacctcca
     1261 aaagaattcg attttgagta ccgcgatgaa gaaaagaact atcatcttga ccgaggactg
     1321 acaccacaaa ttttttatga taaatacatc ggtgtaaaat tagatgatta tgttagcgtg
     1381 atcaatgcac caacaaaaga caaaccattc aatcgttctt atacagtaga gatgctagga
     1441 aatgtcgttg gcgggaaaga agtcaaatat ctgaatgttg atatggaaac tttcaaaaaa
     1501 ttagcgattg ctcagcttga agaaggagaa tctgtttggt ttggttgtga tgtaggtcaa
     1561 tcttctacta gaactagtgg catcatggcc ttagatgctt attcaatgga tgatctattt
     1621 gatacggatt ttacaatgac taaagctgaa cgtttagatt ttggagaaag tctgatgacc
     1681 catgcgatgg tattgaccgg tgttgactta gtagacgggc aatcaacaaa atggaaagtc
     1741 gaaaatagct ggggcgaaaa agttggaaag aatggcttct ttgttatgag tgatgcctgg
     1801 atggacgaat atacgtacca aatcgttgta agaaaagatt tattgacgaa agagcaatta
     1861 aatgcgttca atgaagaacc aattgttcta tctccgtggg atccaatggg tgctttagca
     1921 tagacgagta tttcaatgaa gagtatgtag taaagataaa aagagctggc ttaagccagc
     1981 tctttttatc tttaaagtcc attccctgtt ttttatggtc cctgtgctat aattctaaat
     2041 aaaggataag gattggaaat aatcctttta tcattaaaaa tatgatgacg aagtagaata
     2101 aaattgggag taggggggaa agtcatggat atccaaactg ttttatctga tctgaaatta
     2161 aatggtaaag tgattccagt tgttggtggc gatgttaatc agacctatcg tataaaaacc
     2221 gaacatcgtg cttatttttt aaaaatccat ccgaatgtaa aaaaaggatt ttttgaagca
     2281 gaagtagacg gattaaaaga actgtcagca ttcgtacgtg taccggatac gtatatgcta
     2341 ggtgaaacaa gtgaaggcgc ttatttgtta atggaatgga tcgagccggg aaaaggggat
     2401 caacgagatc ttgctgcagc tttagctaat ctccatcagc aaacagcgcc acaatttggt
     2461 tttcgtaaag ataactattt gggtacactt gttcagaaaa attcttttga agaagattgg
     2521 tggacttttt tctttaaaga tcggcttgaa tcccagattt ctttggcaga agaaacgaat
     2581 cgatggaatg tacagcgtca agaaaagtat ctgcgattta aagaacgtgt attgaaatcg
     2641 gttgaaccaa aaaagatcac tccacgcctc ttgcacggag acctgtggag tggtaatgtg
     2701 ttctttgacc agcaagggca tccagttttt gtcgacccag ctgtttctta tgggaatcgg
     2761 gaacaagata ttgcaatgag ccagttattc ggtggtttcc gaccggaatt tttagatgct
     2821 tatcaaacta tatttccatt agaaaaggga tggaaagacc gactgccgat ttaccagtta
     2881 tattatcttt tggcacattt gaatatgttt ggagaaagtt acggttccca agtagatcaa
     2941 ttactagaaa atttttaaga ggagggcgtg tttttggcaa aaaaatatac caaaaagcat
     3001 gaagtttcat attatgagtg tgacatcaac caaacgatga catttccttc gatgttagga
     3061 gtagttatca aaacgtctga agatcaaagc gaccagttgg gaagaggcag tgaattcgtc
     3121 aatagctttg gacttacttg ggtcatcacg aattattcca tggagattaa tcgtctccct
     3181 agagtcggtg aagtaatctc agtaacgact caagccatgg agtacaataa gtttttctgt
     3241 tatcggaact tctggatcca tgatgaagat gggaaagaat tagtcaaaat agaatctgtt
     3301 ttcgtattga tggattttgt gaatcgaaaa atgagcagtg ttaatgaaga gatcattgct
     3361 ccttttgaga gtgagaagat caagaaaatc aagcgtcagg aaaaaattga aaagattgat
     3421 tcgggcttca tgttgccata tcgtgttcgt ttttatgata tcgatagtaa tcagcatgtt
     3481 aataatgcga tgtattttaa ttggatcatt gacgtattag gatatgactt tttgactacg
     3541 catgttccag aaaaagtaat tattcgattt gataaagaag tagagtacgg gaatgagatc
     3601 gaaagccatt ttgaacaaat ccctatcgaa aatggtgtca aaacaaaaca tgaaatccga
     3661 atgcaaga
//
LOCUS       DAZULY010000232         3657 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000232 DAZULY010000000
VERSION     DAZULY010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.235"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..545
                     /locus_tag="ACG81A_002483"
     CDS             75..545
                     /locus_tag="ACG81A_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFS5015803.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGFPYFDFGWHIFHAKFCDFNSIGKI"
     gene            complement(465..683)
                     /locus_tag="ACG81A_002484"
     CDS             complement(465..683)
                     /locus_tag="ACG81A_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015804.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            790..1104
                     /locus_tag="ACG81A_002485"
     CDS             790..1104
                     /locus_tag="ACG81A_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5015805.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="ACG81A_002486"
     CDS             1208..2386
                     /locus_tag="ACG81A_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5015806.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2567..2752)
                     /locus_tag="ACG81A_002487"
     CDS             complement(2567..2752)
                     /locus_tag="ACG81A_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015807.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            2789..3082
                     /locus_tag="ACG81A_002488"
     CDS             2789..3082
                     /locus_tag="ACG81A_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFS5015808.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(3170..3316)
                     /locus_tag="ACG81A_002489"
                     /pseudo
     CDS             complement(3170..3316)
                     /locus_tag="ACG81A_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT         1253 a          534 c          715 g         1155 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
     2701 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
     2761 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
     2821 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
     2881 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
     2941 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
     3001 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
     3061 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
     3121 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
     3181 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
     3241 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
     3301 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
     3361 cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa
     3421 caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag
     3481 aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc
     3541 agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg acacatttct
     3601 cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat aatcccg
//
LOCUS       DAZULY010000233         3649 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000233 DAZULY010000000
VERSION     DAZULY010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.236"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            40..792
                     /locus_tag="ACG81A_002490"
     CDS             40..792
                     /locus_tag="ACG81A_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS5015809.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            789..1703
                     /gene="pfkB"
                     /locus_tag="ACG81A_002491"
     CDS             789..1703
                     /gene="pfkB"
                     /locus_tag="ACG81A_002491"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS5015810.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1721..>3649
                     /locus_tag="ACG81A_002492"
     CDS             1721..>3649
                     /locus_tag="ACG81A_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFS5015811.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
BASE COUNT         1171 a          659 c          835 g          984 t
ORIGIN      
        1 aaaaaatatg ctattatttg tattgaggtg atcgtgaaaa tgcttacaga ggaacgcaga
       61 caaaaaatcc tagacttgct tgaacagcag aaaatcgtga aatcacaaga tcttgtaaac
      121 cttttgaatg cttctgaatc aacgataaga cgtgatttac aagagctgga gaatagtggg
      181 tttctagaaa gagttcatgg cggagctaag aaagagcaat tattgaattt tgagcagaat
      241 atgagtgaaa aatcactcaa aaacgttcac gaaaaacagc aaatcgccaa atcggcagct
      301 gaaaccatta atgacgaaga tgtcatttat ttagacgctg gctctactac gttagaaatg
      361 attccttttc tgcaaggaaa acagatcaca gtagtgacaa attccgttca acatgcggca
      421 aatttagttg atttggagct tgctaccatt attttaggtg gaacgataaa gctggcaact
      481 aatgctgttt taggatctaa tgcgatagaa caactaaaaa attttcattt taataaagca
      541 tttatgggaa tgaacggtgc ccatcttgaa caaggattca cgactccgga tccagaagag
      601 gcagctgtta aacgtttagc catgaaaaat tccgaagaga catttgtatt gattgatcat
      661 accaaattca accagttgtc cttcacctct gttgcacctt tggaagcagc cacgatcata
      721 accgagcgtt gtccattaga attcgttaat gaattccgtg aaaagacaac aattaaggag
      781 gcaatacaat gatttacaca gtaacactta atccatcaat tgattatatc gtacgcgtag
      841 atgggttaaa actaggcgat ttgaaccgaa tgaaggaaga cttcaagtta cctggaggaa
      901 aagggatcaa tgtttcccgt attttgaaac ggatcgattc tcaatctaca gcactagggt
      961 ttttaggagg gttcactggc gaatttattt cgaagtggct tgaaaaagaa caaatccagt
     1021 ctttgttcac aaccgtaaaa gaagatacaa gaatcaatat caaactaaaa tcagacactg
     1081 aaactgagat caatggctta ggcccatcta tttcagatga agaaatcgat gagttgaagc
     1141 aagcattagg aacagtagga caaggagata tcgtcgtatt gtcaggaagc gcgccagcta
     1201 gcctgcgaaa aggattttat caagaactga ttgatatcat tcgagaaaaa ggtgcagatt
     1261 tcgtgattga tacaacaggt gatgacctaa aaaatgcact tgttaagaaa ccgttgttga
     1321 tcaaaccaaa caatcacgaa ttagctgagc tttacaacac aaccttccaa tctgtagaag
     1381 aaatcattcc gtatgggaaa aaattactag ctgaaggggc acagaatgtg ctgatctcaa
     1441 tggctggaga cggtgctttg ctcttcacgg aacaaggaac ttataaaacg aatgtattag
     1501 ttcgcccatt gaaaaattca gtaggtgcag gggattcaat gattgctggc tttattgggg
     1561 cttacagccg tacgaaagat ccaatcgaag cctttaaatg gggagttgct tgcggaagcg
     1621 caacagcttt ttctgatgac ttggcagctc gcgaattgat tgatgagttg ctgccagaag
     1681 ttgaaatcac aaaaattgat tagtttcaag gaggataatc atgaatatca aggatttaat
     1741 ggtcaaagaa gcgatgatca tggatttgca agcgactgat aaaaaaggcg caatcgatga
     1801 aatggtccaa aaaatgtttg atgccggccg gatttccgat atcgaaacat ataaagaagg
     1861 aattctagcc cgcgaagcac aaacttctac aggtcttggt gatggaatcg ccatgcctca
     1921 cgcgaaaaac agtgcagtta aagaagcaac agttttattc gcaaaaagcc aaaaaggagt
     1981 agattatgaa gccttagacg gacagccaac ttatctgttt ttcatgatcg cagctccaga
     2041 aggcgcaaac gacacacatt tgcaagcatt agcagcacta tctcgattgc tgattgatgc
     2101 agatttcgta ggaaaattaa aagaagcaaa tacaccagaa gaagtccaag acttgttcca
     2161 aacagctgaa gagcaaaaag aagcagaaga aaaagcagaa caagaaacac aagctgcaga
     2221 agcttctgct catccagaaa gaaaattcgt tgttgccgtt acagcttgtc caacagggat
     2281 cgctcacact tacatggcag aagatgcttt gaagaaaaaa gcgaaagaaa tgggtgtgga
     2341 gatcaaagtc gaaacgaacg gttcagaagg aatcaaaaac cgattgactg ctgaagacat
     2401 cgcccgtgca gaaggtgtaa tcgttgcagc agacaaaaaa gtagagatga atcgctttga
     2461 cggaaaagaa ctgatcaatc gtcctgtcag tgatggtatc cgtaaaccag aagagttgat
     2521 caatctagcc cttagtggta ctgcaccagt attccatggt gatggaaaag aaagcagttc
     2581 agaagaagga aatgcggatg gtactatcgg acaaagaatt tacaaagatt tgatgaacgg
     2641 cgtatcacac atgttacctt tcgttattgg tggaggaatc gctattgcgc tttctttcat
     2701 gatcgaccaa tttattggcg tgccacatga tcaattatca aatctaggga attacaatca
     2761 agctgctagc tggttcaacc aaatcggaca agctgcattt ggtttcatgc tgccggtatt
     2821 agcaggattt attgcttcaa gtatcggtga ccgccctggt ttgatcgttg gatttgctgc
     2881 tggtgcatta gctaatacag gcggtgcagg attcttaggt gcactgattg gtggattctt
     2941 agctggttat gtcatcgtat ttttacgtaa attattcaag aacttaccaa aatcattgga
     3001 tggaattaaa acgatcctct tttatccagt gtttggctta ctgattactg gtttcttgat
     3061 gttgctggtc aatgtcccta tgaaagcaat caatgatgca ttgaacggtt tcttgacaag
     3121 tatgagtggt tcgaatgctg ctttacttgg tgcattatta gctggaatga tggcggcaga
     3181 tttaggcggt ccaatcaaca aagccgctta tgtgtttggt acagcaactt tagctacgac
     3241 tgttgcaact ggcggaagtg tcgtgatggc ttctgttatg gctggaggaa tggtacctcc
     3301 attggcaatt ttcgttgcta cacgtttgtt taagaataaa ttcagcaaaa cagatcaaga
     3361 tgctggtttg acgaacatcg tgatgggact atcgtttgtc acggaaggat cgattccatt
     3421 tgcagcagct gacccgattc gtgccattcc aagttttatt attggttctg ctttaactgg
     3481 cggattagtc ggagctttcg gaatcaaatt actggctcct cacggcggga ttttcgttgt
     3541 attcttactg agtcacccgg tcatgtattt ggtgtttatt gctatcggag cagtcgtatc
     3601 tggtgtgatt tatggtttat tgagaaaagc tcctgcagta gcagaagca
//
LOCUS       DAZULY010000234         3619 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000234 DAZULY010000000
VERSION     DAZULY010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.237"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..932)
                     /locus_tag="ACG81A_002493"
     CDS             complement(<1..932)
                     /locus_tag="ACG81A_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HFS5015812.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENF"
     gene            complement(936..1346)
                     /locus_tag="ACG81A_002494"
     CDS             complement(936..1346)
                     /locus_tag="ACG81A_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5015813.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(1364..1855)
                     /locus_tag="ACG81A_002495"
     CDS             complement(1364..1855)
                     /locus_tag="ACG81A_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS5015814.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(1865..2656)
                     /locus_tag="ACG81A_002496"
     CDS             complement(1865..2656)
                     /locus_tag="ACG81A_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS5015815.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(2649..3422)
                     /locus_tag="ACG81A_002497"
     CDS             complement(2649..3422)
                     /locus_tag="ACG81A_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFS5015816.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
BASE COUNT         1125 a          763 c          494 g         1237 t
ORIGIN      
        1 ttaaaattct caattatttt ttcaggcagt ttaacaccaa gagttttgtc tgtcttttga
       61 ataatttgat gtggaacaag tgcaccgccc caatcccagt tattttgatc gttctcccct
      121 tcttttgttg ccacccagcc agttagaaaa cggccagttt cattagttcc gactgttctt
      181 gctgcataat aggcttttcc gtcgaaagct tcgtctactg gtgatttcca atctgaaaat
      241 aatcctgctc cgactcgata tttagtcttt ttatcttcac aatactctga aaaaagtaaa
      301 taccacgttc catcaatttc aaatatatca ggcatttcaa tcatgttaaa ttcattatta
      361 gcccagtagt ctcctttgaa attccaatct tttaaatctt cggattcaaa atagaccaat
      421 ctccccgtcg gctttttctt acttgctggt tttctagctc ctagtatcaa aacgtatttt
      481 ccatgatctt gatcaaaaat tgcaaaagga tcgcgccaat cattcttatc atatccttct
      541 tgaggaacaa gttttgctgc ttcgccatcc tttttccaat gaattaaatc tttagaactt
      601 gcaatcatta acacttctga agtttttcca tccaacttag ctttgcgatt atgtccagta
      661 tacaaagcta aatactcatt gattccttgg acaatacttc cagcgtaaat gtattgatca
      721 atgtcatcgt tgccaccacg atgaattact tccccgtatt cttcaaattt cacaaaatca
      781 gtggttgtaa ctagtgacca cccaaaagga tcagttaaag gtttaggatt tctcgtatct
      841 cgttgatgaa atagataaaa agttccatct ttataaaaag gcatgcaatc cccaaaccaa
      901 aaatctttag gcttgtaaaa aattttttgc atttatcact catcctcttc gttagtatgt
      961 ctttgcatat ctgcaagaca cgcaattgta ttcatatttc ttagattttc gatgatatca
     1021 tcgctttttt ttccttgtaa tctcaaattg acagcctcaa tcaaaattcc taaattcatg
     1081 ccaccaagaa gttgaaaccc attttgaaat acttcacttc ttttagcaaa ttcgttgaaa
     1141 ggtgttccac caatcaagtc agcaaatacc ataacttctt gattttttac ttgagaaact
     1201 attttttcaa tgttttcacg gaattcttca atgcctgcat ctccaagtgt taccacatga
     1261 caatttaaat tatcgccaaa aaacattttt aatgtatcta caaatccaat ggcaatattt
     1321 ccatgagatg taattataaa ctccatccac tcatcctctg ttcttaataa ataaaatcct
     1381 ttaaaaattc ttttgaatct ctaggagttt gtatcactgt cacttcaact ccatcactaa
     1441 ttagttcttt aaaagcctct ctctcttctt cagtaatatt tatcgtcgga cggatgcgtt
     1501 ctgttccatt ccttcttgat aaatttccta cgtttacact tgttattggt aaaccgttat
     1561 caatcaaaaa tttaacatcg attggagaaa ctgcaacaat aagaactttt tgtccttcat
     1621 atttaccaga aagaatattt ttaactgcta cttcccttgt aattattgaa gttctaacat
     1681 taggtggtgc agcgaggcga actacagctt tttttacgtc atcattggct acttcattat
     1741 taataaccat aattctgtta gcactcaatt gatttgtcca ttgtgttgct acttgtccat
     1801 gaatcattcg atcatcaata cgaacatgta taataccttt cattttttca tccatattca
     1861 acacctactt caaaatattt aacgctctta acgcaataga aactactaag attaatagaa
     1921 ttacataaac tggtttaacc tttcgtccta acaaataata cacaattgct actacacatg
     1981 caggtaataa accaggcata acttgatcaa agatttcatt tccggtcatt gttaagtcgc
     2041 cttgtttaaa ttcaaagcca acattcactt ttacaactgt ggcaatgagt gcaccgacca
     2101 ccattaaccc taaaattgat actgcatcag taatagactt taattttgtt tcaatttgag
     2161 ttagtaggct cgcacccgat tctttcccag caaggtataa aggataagca gtaatcctta
     2221 ataaaattga tgccagtatc cacaaaataa ttccaacaaa actaccttcc aatgccatat
     2281 tagctgcaat tgctccaaaa atagtccatg gaataactac aaatacacta tctccaattc
     2341 ccgctaaagg acccattaga cctgttttca gagcacttac tgcttccaaa gacttatttt
     2401 tttcttcttt ttcaattcca acattcatac caataatgta aggcgctgta tacggcgtag
     2461 tgttaaaaaa ttgtaagtga gctaaaacgg attgtttcat tccttcagga tcatctttat
     2521 atgtgtcctt gataaaagga agcatagaat acgcataacc caacccttcc attttttcat
     2581 aatttaatga agttgcacca ttgagaatcc accggaaaat tacttgtttg tcgatttttg
     2641 tcagtttctt attcatcttc gtcaaaacct cctgttgcag ctggtgctac ttgagtatta
     2701 tttgatctat ttttatatac gattagtgcg attgcagcgc caagtaacga aatccctaaa
     2761 attggtattt ggaaataaac tgccaaaacg aaaccaacta acaaataggc aaagtaatct
     2821 tttgtaggta aatatttaag tagcattcct atcccaacag caggtaatag tccccctgct
     2881 acctttaaac caccggtaac ccattcagga atataattga ttgcacttcc cactaacgtt
     2941 gggcctaata tcacagttaa caaaacagga attccagata caaagcttgt attaattgat
     3001 gccaaatatt gcatccactc taatttttga taatcttctt tttgtgcgaa tttttctgcc
     3061 ttttgttgaa aaaaaatgtt tgttgaccac tttagaacat ctagttgtac aattaacatt
     3121 gctaccggta ttgctaaagt tattcctata gaagcatcct ggttagttga gattgctaaa
     3181 taagtaccga tcaatgctgc tgtttgataa tccggtactg atgcaccacc aaaactagta
     3241 atacctagtg tcattaattg caaagtccca ccgatataaa gtccagtttg aatatctccc
     3301 ataataagtc cggtcaagaa acctgcaacg acaggttggg aggttccgat tttgggccct
     3361 tccttatccc agttaatgaa aaatccatac aacacaacta aaaatgtttg ccaaaacgcc
     3421 attttgttct ctcctttctt cttatacttt tatataagca attactgtgc caactaacat
     3481 tatccaataa agcgggaaaa tacagtaaat attaattaaa tgagcgtcta aatatacttt
     3541 ggacaccctt tcattttttt agacgctagt atgctattta gaaaatagtc tgctcaaaaa
     3601 ttgtatagat taactcata
//
LOCUS       DAZULY010000235         3545 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000235 DAZULY010000000
VERSION     DAZULY010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.239"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            126..1892
                     /locus_tag="ACG81A_002498"
     CDS             126..1892
                     /locus_tag="ACG81A_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFS5015817.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            1907..2344
                     /locus_tag="ACG81A_002499"
     CDS             1907..2344
                     /locus_tag="ACG81A_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS5015818.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            2357..3508
                     /locus_tag="ACG81A_002500"
     CDS             2357..3508
                     /locus_tag="ACG81A_002500"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFS5015819.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         1187 a          632 c          768 g          958 t
ORIGIN      
        1 gaaatctttt tattttgctg acatactcaa acctttggca caaagtttcg tgccaaagta
       61 cgactatttc tctttttagt aagcggatac aattagagta tcaaaaggag agagggagag
      121 acaaaatgga acggacgatt cccaaaaaca acatttcgct aaaagcgagg gtacaaaaat
      181 taggcagtac tttatcaagc atggtcatgc caaatattgg agcactgatt gcctggggtg
      241 tattaacggc tctatttatc ccagatggtt atttaccaaa tgaggctttg gctaccatgg
      301 taagcccaat gctaacttat ctgattcctt tattgatcgg ttatactggc ggaaaagtaa
      361 ttgctggtga ccgcggatca gttgttgggg caattgcaac gatgggtgtg attgtcggaa
      421 cagatattcc aatgatgcta ggcgcgatga tcatgggacc tcttggtgga tatgccatca
      481 agaaatttga tcagctattc caaaaacgga tcaaatccgg ttttgaaatg ctagtcaata
      541 atttttcagc aggtcttatc ggttttggat tagcgttatt aggtttttct gcgattggtc
      601 cagttgtcga tgcattgact caagcaatgg caaaaggtgt agagatcatt ttatctgccc
      661 atttgattcc attgacaagt atttttattg aaccagcaaa aatcttattt ttgaataatg
      721 caatcaatca tgggattttg acaccattgg gaactgaaca agtagcagag gctggaaaat
      781 ctattctatt cttgcttgaa gcaaatcctg gtccaggact tggcgtatta cttgctttta
      841 ctttgtttgg caaaggagca gcaaaatctt cagcaccagg agccatgatc attcactttt
      901 taggcgggat ccatgaaatc tattttcctt acgttatgat gaaaccgtta ttattcttat
      961 cagttatagg cggaggaatg tcaggaagtt tcattttcca attgatggat gctggactaa
     1021 gagcgccagc ttctccaggg tcgatcattg cgattctagc gatgacaccg ttcaattctt
     1081 accttcctgt tattttgggc gtgattgttg ccgcagctgt ttcgtttggt atttctgcag
     1141 taattttgaa agcggatgcg aaagatacaa atgaagactt cgaaaaatct gttcaagaga
     1201 caaaggcagc taagcaagct tccaaaggtg cccaaggtat tcctcaagga gctacattct
     1261 ctggcgtaca aaaaatcatt tttgcttgtg atgcaggaat gggatcaagt gcgatgggcg
     1321 cttcgatctt gagaaaaaaa gtacaggaag cagacttgcc tcaaacggtg accaatcaag
     1381 caatcagcca actgacagat gactcgaata cattgatcgt tactcaggtc gaactacaag
     1441 aaagagcaaa acaaaaagca ccgagcgcgc gttttgtctc agtagaaaac tttttgaatt
     1501 cacctcgata tgatgaaatc attcaagaat taagcagcaa gcagaaaaca tcagtttctg
     1561 aaattgagaa tgagccagca gaagagtttc tttcagcaaa catcaaacaa atcaaagaga
     1621 ttctacttgt ccacgatgat cgttccggat cagcaacaat ggggatgacc gtgctcaaag
     1681 atatattaga caagaaccat ttaactatcc cgttaagaaa agtcaatatc caagatttaa
     1741 aagatgaacc tgattcactg atcgtagcca aagaaagttt agcggaaaaa gtacagatgg
     1801 ctgcacctca atccattcat ttatcagtca acagcatggt caatccgcaa aaatacgaat
     1861 cgattgcaca atatttgaaa cagacagctt aagggaggaa aacagcatgc aaagtttgcc
     1921 attcgacatg atcgagctca atcgatcttt taaaaccaaa gaagaagcga tccgctattg
     1981 cggtcagatg cttgtagaag cagggtgcgt tgatgaggaa tatatcgatg ccatggtaga
     2041 tagagacaaa atgttatctg tttatatggg gaattttatc gcgattcctc atggaacaga
     2101 tgaagcaaaa aaatatgtga aaaaatccgg catctgtgtc gttcaagtac cagatggtgt
     2161 agattttggt accgaagaag aagaaaagat tgcgactgtt ttgttcggta ttgcaggagt
     2221 cggagatgag catttgcaac ttgtacagca aatcgcactt tattgcagtg atatggacaa
     2281 tgttgtccag ctagcagacg cgctcagcaa agaagaagta acggaaaatc tagcaattgc
     2341 ttaaggagag agaaaaatga gagcagtaca ttttggtgca ggaaatatcg gtcgaggatt
     2401 tattggagag gttttggcag ctaatggatt tgagattact ttcgtagatg tgaatcacac
     2461 gatcattgat gctttaaatg aaagaaaaga atacgaaatc gaattagcag atgaatcaaa
     2521 agaaaaaatc actgtaaaaa atgtcaaagg aatcaacaat caaacaaatc cagaaaaagt
     2581 aatccaagaa atcgctgaag cagatttagt tactaccgcc attggcccga atatcttacc
     2641 ttttattgct gagttgattg caaaaggtat ccaagaaaga gaaaaggaag gaagcacaac
     2701 atctttagat atcattgcct gtgaaaatat gattggcggt tcagcatttc tagaaaaaga
     2761 agtgtataaa tatcttccgg aaacatcatt tactgacaaa tatatcggct ttccaaatgc
     2821 agcagtcgat cgaatcgtcc cattacaaca ccatgaagat cctttattcg tccaagtgga
     2881 acctttcaaa gaatgggtca ttgacgatag ccaaagaaaa aataaaaaaa tccaattaaa
     2941 aggggtttta tatgtagatg atctagaacc atatatcgaa cgcaaactat ttagtgtcaa
     3001 tactggtcat gcgactgtcg cttatacagg tgccttgtta ggttaccaga ccattgatga
     3061 agcaatgcaa gatgcgttag ttgtggcaca attaaaatca gttttgcagg aaacaggaag
     3121 tttgctgatt gccaaatggg ggtttgatgc agagcagcat catgcgtata ttgaaaaaat
     3181 cattcatcgc ttccaaaaca aaaatatctc tgatgcgatc acacgtgtcg ctcggacacc
     3241 attacgtaag ttaggttatc aagaaagatt tacacgtcca gtgagagaac tacaagaaca
     3301 taacttgact tgcccacatt tgacggcaac aatggggatt atttttaatt actatgatcc
     3361 tgaagatgaa caaagccgtc aattgcatga aatgaagatc catgaaaact tggaacaatt
     3421 gattcaagaa gtcacaggaa tcaatgatcc gaaaacaatc gggaatatca agcaaaacgt
     3481 gaaccgttat gccaagcaag tcgcttaaaa taggatacga aaaaggggaa tgtgacgaaa
     3541 ttttt
//
LOCUS       DAZULY010000236         3481 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000236 DAZULY010000000
VERSION     DAZULY010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.240"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..1793
                     /gene="yfcC"
                     /locus_tag="ACG81A_002501"
     CDS             54..1793
                     /gene="yfcC"
                     /locus_tag="ACG81A_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFS5015820.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1950..3134
                     /gene="iadA"
                     /locus_tag="ACG81A_002502"
     CDS             1950..3134
                     /gene="iadA"
                     /locus_tag="ACG81A_002502"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFS5015821.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3245..3448
                     /locus_tag="ACG81A_002503"
     CDS             3245..3448
                     /locus_tag="ACG81A_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS5015822.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
BASE COUNT         1104 a          586 c          814 g          977 t
ORIGIN      
        1 ttatccattt tttattcatc aaaattactg aaaaaagaaa ggaagaaata aatttgaaga
       61 aaacaaattc ttcaggaaaa ccgaaacgcg acttcactaa attatcgaca ccccatacgt
      121 atgtcattat ttttggtgta gtcatctttg catggatact gacttttgtc gttccagcag
      181 gaaagttcag cacgcaagat attgaataca aagatgcaaa tggcgaaaca agtactcgta
      241 ctgttttacg tcaagattcc ttccgttatg cttacgagct ggataagtcg tatgtgtttg
      301 atcaattaga agaactgcaa gatcatccag cagaacgtga aaaactggat gttccggaaa
      361 aaggattgga aaaagtcatt gctgacggag aaaaaaatct tacgcaggaa aaactagatg
      421 aaatctcctt gacagatgat gtgctttacg atcagtacgg cgaaaatatc tatgatactt
      481 ctaaaaaatt gcataaaaca gccaaaatat ggggaacaga tgattttggc ggctttggtt
      541 tcttaaattt tgtttttgaa ggactagtat ctggagataa gtatggatct gctgtaggga
      601 tcgctgcatt gatccttgtt gttggaggtg cttttggtat tattatgcgt accggagcga
      661 tcgacgcagg tatctatgcg tttatcagta aaactaaggg actggagcgt ttagctttac
      721 cgctattatt ctttgctttt tcattcggtg gcgctacatt cgggatggca gaagaggtaa
      781 ttccattctc aatggtgatg gttccatttg tcattgcgtt aggatatgac tcgatcgtag
      841 cagtaaccgt gacttatgtc gcatctcaag tcggaaatgc gacctcttgg atgagcccgt
      901 ttagcgtagc ggtggctcaa gggattgctg gaatccccgt gttgtctggt gcgacattcc
      961 gattgattat gtgggtagtt gttactgcat tagccgctgg ttatatgatg atatatgcag
     1021 aaaaaatccg taaaaaacca gaaagttcgt tgacttacaa atcagatgat tatttccgtt
     1081 ctcatatcaa aaagacatct gatgaaaata aaaaattcat gcttgggcat aaattgatct
     1141 tattagagat gttagttgtc cttgtctgga tcgtttgggg cgtgactcaa aaaggctatt
     1201 acattccaga aatcgcttct caattctttg tgatgggatt agttgctgga atcattgctg
     1261 ttcttttcaa attagatgga atgaaagtca atgacatcgc ttcttctttc caatctggtg
     1321 cagctgattt agctggtaca gcaatcgtag taggtatggc caaagggatt cttcttgtct
     1381 tgggaggatc tgatgcatct gttccttcag ctttgaatac gattttgcat gggatcggga
     1441 cggcgttgac cggtgttcca gctgtcattg gtgcttgggc gatgtacatc ttccaaagtt
     1501 tgttcaactt ggttgttaca tcaaattcag gacaagcagc tttgactatg ccaatcatgg
     1561 ctccactggc agatttagta ggtgtatcca gacaagtagc agttcttgct tatcagcttg
     1621 gttcaggttt tatggacgct tttacacccg tatcagccag cttgattggt gtgctaggtg
     1681 ttgcacggat cgaatgggca aaatgggcaa gattccaaat caagatgcaa ggattcctgt
     1741 ttgttttagg aactattttc atcatgattg ccattgcaat aggtctgcag tagtactgga
     1801 gacctgtaaa taaaaaagta atccaaacag tagataagga ctccaagaac cgtcatttta
     1861 cggtaaaatc aaaggagtaa agatcctatc gtttggatta ttttttaaga tcctatcatc
     1921 agttatactt aaagaaagag gaaaaatgta tgaaaatcat tcgacaaatt gaagtgtttg
     1981 caccagatta cttaggaaaa atggatgtac tgattgccgg tgggaagatt ttggctgtag
     2041 aagaacagct agaaggcggc tacgaaggag tggaagtaga agaactttca ggtgagggaa
     2101 aagtactaac cccgggtttc attgactgtc atttccatat tttaggtgga ggaggagaag
     2161 gcggttatca aaatcgaacg ccagaagtca cattgagcca attgactaca gctggtgtaa
     2221 cgacagttgt cggttgtttg ggaacagacg gagaaggtag agatatgact gcgttgatca
     2281 gcaaagcgaa aggtctggaa gcagaaggaa tctccactta tgtatacgaa ggttcgtacc
     2341 gattgccggt aaaaacagta acagattcta tcatcaaaga ctttttgaca attgataaaa
     2401 ttatcggaat cggagaaatc gcagtatccg atcaccgttc ttctcagcca agttttgaag
     2461 agttcgctcg tgcagtagca gatgctcgtg taggaggcat gttatccgga aaagctggga
     2521 tcatcaatgt ccacttaggc ggaggaaaaa gaaagctaga gctattgaca cgaatcgtag
     2581 aagaaacaga aattccgatc acacaatttt tgcctactca tgcaaatcgc acaccagaat
     2641 tatttgaagc ttgcgtggca tttgccaaac gtggtggcac gattgacttc actgctagcg
     2701 aagatcctga tttttgggag aagacagatg gtgaagttcg cttcagcaaa gcactgaaac
     2761 gattgatcga ggaagatgtt tctttagata atttcacgat gacctcagat ggacaaggaa
     2821 gcttgcctta ttttgatgaa aacaatcatt tcctagggtt aggagtagga agtgccaagg
     2881 cattgctagt agggatcaaa gaagctgtgc aaaaagaaag tatccctttg gaaatcgcct
     2941 tacgagctat aaccagtaat ccagcacgta tattgaaatt ggataaaaaa ggaaaaatcg
     3001 aaattggtgc tgatgcagat ctgtgtatct tggataaaga aacgcttgat attgatacag
     3061 ttatcgctaa aggagaaata atggtccaag aaaaagaagt caaagtatgg gggactttcg
     3121 aaaaaagttt ttaatcaaca gctctaacta tcttttttag aaaacgagcg ttctgtttgt
     3181 ctgtatctgt tgttgatgtt accctgaagg tagatcaata aacaaactaa gaggaggaag
     3241 cacaatggca gatttaaaag gacgttctaa agatatgaaa gataaagtag tcggtgaagg
     3301 aaaagaagca tacggcaaag caacagacga caagagcaaa gaaacagaag ggaaagcaca
     3361 atcactaggt gctgacatca aagaaaaagc tcgaaattta ggcgacgaag tcaaagacaa
     3421 gttttctcga aaagacgaaa aaaaataaaa accacttatt actaattgaa tcaagcaaat
     3481 a
//
LOCUS       DAZULY010000237         3466 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000237 DAZULY010000000
VERSION     DAZULY010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.241"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACG81A_002504"
     CDS             complement(<1..2232)
                     /locus_tag="ACG81A_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFS5015823.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACG81A_002505"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACG81A_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          973 a          768 c          548 g         1177 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg aatccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacact tttataaaac gacgaaaaat aaatcactac cagtca
//
LOCUS       DAZULY010000238         3445 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000238 DAZULY010000000
VERSION     DAZULY010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.242"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(748..2202)
                     /locus_tag="ACG81A_002506"
     CDS             complement(748..2202)
                     /locus_tag="ACG81A_002506"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015824.1"
                     /translation="MFDESLHLFPEYPDTVGSQAIPEKKQAVCQILLKEEVQLKQELT
                     AVILMDRICKEVLAGSDGTLAYGVVPLFDEVVRLLGNTQQIGAICGEFLLLPKMLVTV
                     ELAELATGMNEHPYMSGYFYLTVDDKNRQALMQAVTRLLTEKKLNILVGTVSLLGEGW
                     DCPAINLLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVPLSEQPDLK
                     RMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSCERSRLKEQ
                     WSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKGNLLEYFQI
                     RRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLENAGYREGQ
                     LFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQSSLPKCQLV
                     YTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(2203..2502)
                     /locus_tag="ACG81A_002507"
     CDS             complement(2203..2502)
                     /locus_tag="ACG81A_002507"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015825.1"
                     /translation="MESQETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHN
                     YELIPEHWQLLGSSKQQGKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(2805..3176)
                     /locus_tag="ACG81A_002508"
     CDS             complement(2805..3176)
                     /locus_tag="ACG81A_002508"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS5015826.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
BASE COUNT         1057 a          736 c          556 g         1096 t
ORIGIN      
        1 agtcacttta tattactttc tcttgagtaa aaaaacatgt gaggtcgtaa acaaagattg
       61 aactatctac attcatactc tataatctgt ttatttgcag actgataata gcaaaagaaa
      121 acttataatc aaataatatc aacatttgat cacataattt gagattgaat gctgatttac
      181 ctatgtttta cgtaatggtc tttagttatt tttgctcata aaatgcataa aacacgcgtt
      241 tttatgtgtg cttgaaattt cttggctcta taatttctaa gactgataaa aatattctat
      301 cggttctttt tgcaaataaa aaaacatata gaagctccat tagattgaat caccacaaca
      361 caatcaaagg agcattccgt atgcttgatt ttcattctat aaaactactt ttaggaatca
      421 aagaaaatca caacggttca aactaaaagc aagaatatcc gaagtaccat tgtttcatcg
      481 atcatgagaa aagcattcac ctctgtctgc agatgttctt atatcgctct gtcagtccta
      541 ttaataaagt actgttttgt tttcaaaaaa actattacga gtacttttta agagaatgcc
      601 ttgcaaatat tcatcacaca ccgttatata agtcaaatag tttctttcag tcgtcaagaa
      661 aagattctcc tgtccttggc attatcagtt aagtagcaac ttaattctcg gacttgttct
      721 actctagcaa aatttatttt catcaactta cgtccaaatg tttttttgaa taattacttc
      781 atcgcgggtc tggaagactt gcctcattcg ataagtcact agcttttggc gtccttcagg
      841 atttctagta tacaccaact gacatttggg cagcgacgat tgatttaatt cgtttagaaa
      901 aaggttagcg atttgcttat ttcggccaaa gcgttctggt accgtcagaa ctatcttttt
      961 cccttcgatt aaataccgcg gattctccag cggtgctaaa agctccctca tagcttcagt
     1021 aaaaagctgt ccttctcgat agccagcatt ctcaagatag catctaaatt cctgctgatc
     1081 agccgctacg attacttttc tatcactaac ttcaccaagg cgctgcaacg tcgtttgaac
     1141 gcatatggcc aagctttttt tgcgggcaca ttccttttcc catagctttt gtcgcctacg
     1201 aatttggaaa tattccagca agttaccttt aaatatagct gaccagaagc ttagaggctc
     1261 tatatttttt actactatcg gcaatgaagt ttcttgtttt tgctttacct gatcgacttt
     1321 cctaacgacg attggcatag ataggtgcca gccatcagct aaagcagtac tccattgttc
     1381 tttcaaccga cttcgttcac aagaaaaata gaacatcgac tgagtataag tttccatcgt
     1441 ggtggtcgta tattccagtg gtaaagcaaa gcgatcgcca ccactctcaa tttttggtga
     1501 agattcaaag gtcagacctg aaatattgcc caaacgccgt agcattcttt tcaaatcagg
     1561 ctgttcagac agaggaactc ccggtaatcc aatcgctaaa tgccaaatgt tcgttacttt
     1621 attttgtgca cctaggcgta agccccgccc tcgtagttgt tgtgtttgca caaaagtgga
     1681 agcttgatta gccaagatca acagattaat tgcaggacaa tcccagcctt ctcctagcaa
     1741 actaacggtt cctaccaaaa tattcaactt cttttcggtt aataggcgag taactgcttg
     1801 catcaatgct tggcggtttt tgtcatccac cgtcaaataa aaataacccg acatataagg
     1861 atgttcattc atacccgtcg ctagttctgc cagctcaaca gttactagca ttttcggtaa
     1921 cagtaagaac tctccacaga tggcaccgat ttgctgagtg ttacctaata gacgaacaac
     1981 ttcatcaaac aaaggaacca cgccataagc taaggtacca tcactgcctg ctaatacctc
     2041 cttgcaaata cgatccatca aaatgaccgc tgtcagttct tgcttcagct gaacttcttc
     2101 ttttaacaaa atttgacaaa ctgcttgctt tttctcagga atcgcctgac taccaacagt
     2161 gtctggatac tctggaaaca aatgcagcga ctcatcaaac aatcatccag cttttttcaa
     2221 ttgataaaaa ataggcaatt ccggttgatc ttcaaacatc tgctgataaa tagtgatcag
     2281 ctctttttta cctaaagatg gaactgaagc ttttttacct tgctgcttag aactgcctaa
     2341 tagctgccaa tgttcgggta tcaattcata gttatgatga ttcaagtaaa ttaatgcact
     2401 agtgaacagc tcaaaatttt cataaatttc cttcgaatgc tcaagcggat gctgaacaaa
     2461 ttctaaagaa agcaaataat ccaccgtctc ttggctttcc attaacgctt ccaccagctc
     2521 attttgtttc tctaaaaagg taaaataatg ctgagtagtt tcctgattag ccggaatcaa
     2581 atatagaaaa tcctgatgag gacatagaac ctcctccttt accaactcgg gaatgctaat
     2641 ttcttcgtca atcaggccat tttaactcaa tatattgctt ccactccgtc tgagagctat
     2701 caatcggagg cgtggctgtc aaggaaatcg tcaacagatt cggttgtttt ttcttcaagt
     2761 caatcaacaa ttcaccccag ctttttttta aatgatgagc ttcatcaaaa aactaacagc
     2821 tccagctgat caccataagt tggctcctcc ttcagtcggt tgtaaatggt ctgatacgtt
     2881 tccactgtaa tatgactagg atgttcaatc gccccagaaa gatagcaact atctgctgga
     2941 tttactaaga aatattcctc gattgttgtt aaccactgtt tcttcaatat tagggaagga
     3001 accaaaatta aggttttaca atccaaacgt cggatcatct caattcctaa aaaggtcttg
     3061 ccagcccctg gtggagccac tagatggact gaattgttat ttaaataagt agcaaagtta
     3121 gtcaatatac gcttctgata gctacgccac tgaatttttt catcagatag ccacatccca
     3181 ggtccccctc tcacgtattt tctctatcat aatctaaatg aatacagcaa aaaaaagatt
     3241 tcttcaagaa tatgtgtttt tatagtaata ttttacttac tctaaactca aatgaaaagg
     3301 taaaaaaact ggtagtacaa tcttttaaat tctcattgtt ttgtacgttc tttactatca
     3361 aaaaaacaaa aaaaacgcgt ttctacgcgt gaccaaaatt tttagctcta taatttctaa
     3421 gactgataga aaatcattct gtttt
//
LOCUS       DAZULY010000239         3346 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000239 DAZULY010000000
VERSION     DAZULY010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.243"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1309
                     /locus_tag="ACG81A_002509"
     CDS             <1..1309
                     /locus_tag="ACG81A_002509"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFS5015827.1"
                     /translation="LFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQS
                     KKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYT
                     PHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTT
                     TYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTF
                     LDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKL
                     LEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSE
                     ENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGND
                     IKKIRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1299..2516
                     /locus_tag="ACG81A_002510"
     CDS             1299..2516
                     /locus_tag="ACG81A_002510"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS5015828.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2529..>3346
                     /locus_tag="ACG81A_002511"
     CDS             2529..>3346
                     /locus_tag="ACG81A_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFS5015829.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQV"
BASE COUNT         1156 a          571 c          691 g          928 t
ORIGIN      
        1 tcttttcaac gtcttagcgg atcaagcaaa acaagcaacg ttccaattga acgatctaaa
       61 caaagccttt gtgcaattgg catctaccta caatcaattt aatgggttat ttgatgatgt
      121 ggatctccaa tcaaaaaaac taggaacaga tgagcaacaa cgaaatgtga cgattaccga
      181 agtcatcaag aaattgaatg atgtagatgt tttagaacac gatggggacg tgatcgggga
      241 tgcgtatgag tttctgatca gtcaatttgc ttcagaagca gggaagaaag ccggagaatt
      301 ttacacacct catatggttt ctgatatgat ggcgcaaatc gtaacgctcg atcaaaagga
      361 acgacgattc tttagtgttt ttgatccaac gatgggttca ggttcattga tgttaaacgt
      421 acgtaactac ttaacacacc cagataacgt taaatatcat gggcaagaac taaatacaac
      481 gacatacaat ctagcaaaaa tgaacttgat tttacatggt gtcgacgccg aagaaatgaa
      541 tctccgcaat ggggatacgt tgaataagga ctggccaaca gatgaacctt acacatttga
      601 tgcagtagta atgaacccac cttattcggc taattggtca gcagatacga ctttcttaga
      661 tgattctcgg tttaatcgtt atgggaaatt agcgccaaaa tccaaagcag attttgcgtt
      721 tcttcttcat gggttctatc atttgaagga aacaggtacc atggccatcg tcttaccaca
      781 cggagtttta ttccgtggcg ctgcagaagg ggtcatccgt caaaaattac tagaagatgg
      841 cagtatttat gctgtgatcg gtatgcctgc gaacttgttc tttgggacat cgattccaac
      901 gacggtgatt gtgttgaaga agaatcgtca aactcgagat gtattattca ttgatgctag
      961 tcgtgaattt gttaaaggga aaaatcaaaa taaactatcg gaagaaaaca ttcaaaaaat
     1021 cctcgaaacc tacgctgaaa gaaaagatgt cgagaaatac gctcatttgg caacctttga
     1081 tgaaatcaaa gaaaatgact ataacttaaa tatcccacgc tatgtagata ctttcgaaga
     1141 agaagaacca attgatatgg ttcatgtcgg aaatgacatc aagaagatac gacaagaaca
     1201 gcaagtgcta gaaaaaaaac ttttagaagc tatctcttcc ctgcaaacaa cacctgaaaa
     1261 tgaagcgtgg ttacaaggtg cgctggaggt gttcaaacat gagcaatgat acacaaccag
     1321 aaatccgttt cccaggattt acagatgatt gggaacagag gaagttgggg gacttcgttg
     1381 tagattatgt tgaaaagaca agtgtacaaa atcaatttcc tatgctaact tcatctcaac
     1441 aaaaagggat agttttgcaa gaagactatt ttgcaaatcg gcaagtaact acagaaaata
     1501 atattggtta ctttgtacta cctagaggat atttcacgtt ccgtagtcga agtgataatg
     1561 atgtgtttgt gtttaaccgt aatgacatta ttgatagagg gattataagc tatttttatc
     1621 ctgtatttac tttaaaatct gctgattccg acttcttctt aagacgtatt aacaatggaa
     1681 tccaacgaca attatctatt caagcagagg gaacaggtca acatgttctg tctctaaaaa
     1741 agtttaaaaa tatagtagca atgtttccta gtgaggaaga acaacaaaaa atcggcacct
     1801 tcttcaaaca acttgacgac acgatcgctc ttcatcagcg taagttagat ttgctgaagg
     1861 aaacgaaaaa aggattctta caaaaaatgt tccctgaaaa cggagcaaaa gtgccagaaa
     1921 tccggtttcc tggttttacg gaagattggg aagagcgtaa gttgggagaa ttaggtaaaa
     1981 ctcaatcggg aattggattt cctgatgccg agcaaggtgg ttctgaaggt actccatttt
     2041 ttaaagtttc cgacatgaat aacattggta atgagtatga aatgagaaat gcaaatcatt
     2101 atgtatctaa tgagcaaatt gagcgtaaaa aatggaagcc aataaaagat gtgccagcga
     2161 ttatatttgc taaagttggc gcagcaataa tgcttaatag aaaacgtttg gtcacaagcc
     2221 ctttcttaat agataacaat accatggctt atttatttaa taatacttgg gatatctatt
     2281 ttggtaaaat attgtttgaa actataaatt tgccaagata ctcccaagta ggggcattgc
     2341 cttcttataa cagttctgat attgagaata tcagtgttaa ggttcctgta aaggatgaac
     2401 aacaaaaaat cggtactttc ttcaaacagc tagatgatac gatcgctctt catcaacgta
     2461 agttagattt actaaaagaa acgaaaaaaa gcttcttaca aaagatgttt gtctaggagg
     2521 gaagaagaat gaaaattagt caccgtgagg aagcagaagt cgaagaacag ttgattcgtg
     2581 tacttggaga agggcataat cagtggacgt atcgtccgga tctaaaatca gaagaagacc
     2641 tttgggtcaa tttacgccaa aaaattatct caaataatca agcagaactg aatgattttc
     2701 ctttgaccga taaagaattt gaaacgatca aaacggaatt actgctgcga acgaaaaccc
     2761 catttgatgc tgcgaaatgg ttaaaaggtg aaaatgggat ggctcggatc acgattgaac
     2821 gggaagattc gcaattgggt tctgtctcac ttatccttta ttccaatcaa gacattggtg
     2881 gaggaatctc tacttatgaa gtggtccatc aaattgcaaa acgtggaagt aacatagaag
     2941 cacgggatcg acgttttgac gtcaccttat taatcaatgg attaccgatt gtccaaatcg
     3001 agctgaaaca agtaactgca aaagatggtg tttatcaggc atttaaccaa atcaaaaaat
     3061 atgcagaaga aggtatgttt cggaacaata tcttttcgac cttacagttg tttgtggttt
     3121 ccaatgaaca aaccactcgg tattttgcaa atgccttacc gaaagacttg caccctaagt
     3181 ttttatttag ttggcgaacg aaagacaatg aaaaagtaga aaacttatat gagttttgta
     3241 aacaagtgtt aaatatcccg gatgctcatc gtttgattgc ggattataca atcgtcagtg
     3301 aagatcaaga caataaaacc ctcatggtgt tacatcctta tcaagt
//
LOCUS       DAZULY010000240         3335 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000240 DAZULY010000000
VERSION     DAZULY010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.244"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            588..1004
                     /locus_tag="ACG81A_002512"
     CDS             588..1004
                     /locus_tag="ACG81A_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFS5015830.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            1018..1584
                     /locus_tag="ACG81A_002513"
     CDS             1018..1584
                     /locus_tag="ACG81A_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFS5015831.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            1607..2593
                     /locus_tag="ACG81A_002514"
     CDS             1607..2593
                     /locus_tag="ACG81A_002514"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFS5015832.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(2748..>3335)
                     /gene="scm"
                     /locus_tag="ACG81A_002515"
     CDS             complement(2748..>3335)
                     /gene="scm"
                     /locus_tag="ACG81A_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFS5015833.1"
                     /translation="TDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSAS
                     SDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTT
                     SDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRAL
                     PKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT          990 a          625 c          717 g         1003 t
ORIGIN      
        1 ttgttgcttt cgcattgatg aacggttccg gacgtgcgat tgggttggat cgctgggtaa
       61 ttccatggct acaacgtaca ctaggtaatt ggtggtacgg tagaccaagt tcccagtacg
      121 gcaagaaata aatagataaa gaacgttatt tgaatgcaag atttcaatag aaaggggctg
      181 agacaaaccg tcacagcctc ctttttatgt aaaattaaag gaataagatg cccgagataa
      241 gcctccatga gcaagacaca ggtgtcacaa cctctgttac aacctcttgt tataaacggt
      301 gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaactt gaaaagtaat
      361 tttcgtaaat tccttcttat ttcttgaagt tgcccacttc tgtcacaacc tcttgttata
      421 aacggtgttc cacctgctgc ttctcggtaa taagcccaaa gaattagaaa acatggaaag
      481 ctgttttcta ttcttcgttc ttattactca aagcaagaca caggtgtcac aacctcttgt
      541 ttttatgcta aaattgggtg agttaatctg gaaaaaggag aacaaaaatg agccttaaag
      601 aatttgctga aaaaagtctg ctcaaacctt gggatgtttt tattattttg cttttgactg
      661 ttctttcttt tttaccagtc atgatttttg gtcttcagca aaacaccgca acagcagaaa
      721 aagaagcggt actgcgtgtg gatggcacag aaatcaaaac attccctttg atggctggag
      781 aagagagcaa cacatatact tacaaagatg aacacggaga ttataacttg atcgagatag
      841 atggcgatcg agtacggata aaagaagcag attgcgatga ccagatctgc gtacgtcgag
      901 gctgggcaac gaaaaatggc gaaaccatcg tctgtttgcc acataaatta gtgatcgaag
      961 tccgttcaac agatggaggt gacgaagaca gtctgattta ttagaccttc acacaccatg
     1021 agtaaattac aaaaaatgat ttatatttcg ctacttgttg cacaaggagt gatcattggt
     1081 ttattggaaa atatgatccc ttatcctttt tcttttgcgc ctggtgcaaa actaggctta
     1141 gccaatctaa tcacgatcgt tgccattttt accatgaaaa aacaagacag cttcttacta
     1201 ctttggctac gactattcct gactactttg ctaggcggaa cgatttccac attcctttat
     1261 agtatgagtg gtgctttgct tagttatttt ggaatgttat tggtcaagca attaggacca
     1321 aaacgtgtca gtatcatcgg aattagtgcg gccggcggtt tcatgcataa tgtcggacag
     1381 cttttgatgg cttctttcat tgcacaatca tggacagtca tgttgtactt gccagtcctt
     1441 tcagttttag ggattttatc agggattgca atcgggatcg cagcgaatta tcttttacga
     1501 catgtgcaga cacttcaacg attccaaaaa gattatgagc agggaaccaa acaaaaatgg
     1561 agcccaatgt ttaccaaaaa ataatcgtca cacaaggagt ttttttatga agctacatcc
     1621 aatgtgggaa aactatcctg ctttaagtaa agaattgaca gaaacactaa aacggatgga
     1681 agaaactgtc cgcctgaaaa acaaacctgt cgaacaagcc atcaaagaaa cgatccatgc
     1741 cggcggaaag ctgttgcgtc ctgcctatca gctattgttc tcacaatttg gaccagagca
     1801 agatcgagac aaagccatcg ctttggcagc ttctatcgaa atgctccata cggccacact
     1861 gatccatgat gatatcgttg atgaagcgga tctgcgacgt ggtcagccta atatccgtag
     1921 tcagtttggt aatgtcgttt ctgtctatgc aggagactat ttatttgtct gctgttttaa
     1981 actattgtct gattactcca cttccttgaa aagtctccaa ttgaattctc gcagtatgga
     2041 aaaagtattg aatggcgaat tagggcagat ggatgatcgc tataattatg aactttctgt
     2101 aaaagaatat ttggaaaata tttcaggtaa gacagctgaa ctgttccaat taagttgttc
     2161 tgttggcgct tttgaaagtg gaacaagcga gcgcttcgca aaaaaagcag gagatatcgg
     2221 actttctatc gggatggcct ttcagattat cgatgatatt ttagattata caaaagagag
     2281 ccaggaaatc ggcaaacctg tactagaaga catgcgtcaa ggggtctatt ctttaccgtt
     2341 gatctactct ttgcaaaaaa acaaaccaaa gctactacct tatttagaga agaaagcggc
     2401 aatgacagaa gaagatgtcg acgccgtacg caaaatcgtt gaacatacag gaggcgtgga
     2461 agaagctagg aagttagcgg cctcttacac agaaaaggct ttgaaggaaa taaagaaatt
     2521 acctgccacc tctcttcgaa caaaagagaa tctcttttcc cttacccaac tgattttgga
     2581 tcgcaaagac tgaataggga aaggttcgat acgtaaataa atttctcaga aggaaatcta
     2641 cgtgtttttt gacaaattga attgtagttc ttgtatttat gataaaaagc aaaaataaag
     2701 agcaacgtcc gttagcgacg ttgctctttt ttatcttgaa tattttttta ccttttcgat
     2761 ttcttataac tcataaaagt gattcttaaa ccgattacaa gtaatataac gcctgcgagt
     2821 gttatccaat tattcgattg acttccagtt ttaggaagtg ctctttttga tttgtttcca
     2881 ttgtcattgc ttgttttatt agttaagtta aagttcgtcc tgttatccga acttgttttt
     2941 gagaaggaga cacttgcatg acgtgtatcg cttgtggtag acgacgtgtc tgtactatcg
     3001 cttgaagttg atgaatctga gctatcactt gtggtagagg atgtatctgt actgtcactt
     3061 gaagctgacg tgtccgtgct atcacttgta gtggtagatg aatctgagct atcacttgtg
     3121 gtagaggtag tatctgtact gtcacttgaa gctgacgtgt ccgtgctatc acttgttgtg
     3181 gtagatgtat ccgtactgtc gcttgaagct gacgtgtccg tgctatcact tgtggtagag
     3241 gtagtatctg tactgtcact tgaagctgat gtgtccgtgc tatcacttgt ggtagaggta
     3301 gtatctgtac tgtcgcttga agctgacgtg tccgt
//
LOCUS       DAZULY010000241         3283 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000241 DAZULY010000000
VERSION     DAZULY010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.245"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1376)
                     /locus_tag="ACG81A_002516"
     CDS             complement(<1..1376)
                     /locus_tag="ACG81A_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS5015834.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEK"
     gene            1468..2439
                     /locus_tag="ACG81A_002517"
     CDS             1468..2439
                     /locus_tag="ACG81A_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFS5015835.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            2436..3218
                     /locus_tag="ACG81A_002518"
     CDS             2436..3218
                     /locus_tag="ACG81A_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFS5015836.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
BASE COUNT         1087 a          508 c          651 g         1037 t
ORIGIN      
        1 attttttctt ttacgatact tagtccagaa gctaaaattt caaggttatt agaggatagg
       61 gaaattcgaa cgaatgcatc tggttgcgca cgtgtagtaa agcgtctgga atgataaagt
      121 cggataccgt tagcaagaaa gtcattctca attatcttag ggtcaatctc aggatcaact
      181 ggaagaattc gatagtaagg gacacgattc gttggttttt ctactttgaa aagagaatca
      241 aaaagctgat tggctctttt tgtaagactt aatttagttt ctaagatcct atttgcttca
      301 tttgaatgga ggatttgagt gatgacttcc acatctaatc cagaagtttt cacatttata
      361 ttaaaaattg cttgttctat cttgctttgg acatgtactg gaactacaag ataagcaact
      421 cgcaatccag aacaaataaa ttttgtcatt ccagctaagt agattgtttg gttaggtaat
      481 aaattttgga aaggaggaat gatcgtatgt tgatagtaag ttgttaaaaa tgaatggata
      541 tcatcttcaa taacccatag accttctttt tcaatcactt cggctaattg ctctcgtctt
      601 ttgtgagaga tttgaaaccc tataggatta ttacaagaag gcataagaaa gacaccatga
      661 atcttctttt ttctacattc ctgaatcaat atatcaggtc gcattccttg ctcgtcaaaa
      721 tcaattggaa cgatctctaa atgatataac tgagccaatt caatgaaatt agcgtatgta
      781 tatcggtctg ttgctattct atcacctgga gagaaaagtg cagaaagtgt gatagccaat
      841 ccattttgaa caccggaaac aatagataga gattctaagg acggaatgac tccttgagtt
      901 tttaaccaat ttgctgcagt agtcaattga aaatttgtgc ctttgggatc ttggtaatta
      961 agcaaaccaa caagatttgg gttattacta gttgattgaa taaacgggac aacgaattta
     1021 ttacattctt caaaagaact aaccagacca aaatcaatga tatgttcggt tacttcattg
     1081 gtagagatgg tgatggactc aaagacattg ggagaaataa atgtgccact tccaatattc
     1141 gtatacagca aacctttctc aattccatat ttataagcct tgccaacggt agtaaaattt
     1201 aaatctaaat aatcagctag ctctctttgg gagggtaact gagtgttttt ctgtaattta
     1261 ttctttaaaa tatcttgttc taattgttga atcaacgatc gataataagg acgcttcaat
     1321 ttttgtttgt ctggtcgcca agttaacggg taattgtcat aagaattaat aggcattttc
     1381 tccactcctt aaaattgtat accatacaat tttaccattg attgtattga ttttagaagt
     1441 taatcttttg tgaggaggga attgtctatg aaaattatta attttgagga agtagtagct
     1501 tcttttagtt ttgaagaagc gattcaagta atgaaaaaat gtttttttga ttatgaaagt
     1561 ggtaaaatca gccaaaatcc tcgattggtg gaaattttac ctgacggaaa aggcaaaaat
     1621 atgtttgcga tgatgcctgc ttatttgggg gagaacagat attttggcac aaaagtcatt
     1681 accgcttttc cggataatca tcagtatcat ttaccttctc atttaggaga gattttcttg
     1741 tttgatgctc aaaacggact acccaaagca ttgataaacg caaatgcagt cacctggatt
     1801 cgaacagctg ctgtatctgc gctggcaaca gattacttag caaatccgca tgcagaaaca
     1861 cttgctttaa ttggagctgg tcaacaagca ttgtcgcatt tgactgcgat ttctaccatt
     1921 cgtcccatca ctacagtgtt tgtttacgat attttagaag aaagacgaaa tacttttatc
     1981 gctaaagcta aaaaacagta tcctaaaata acgtttataa atgcaatatc agtagaagat
     2041 gcagtacgtc cagcggaaat catttgtacg ttgacctcta gtaaagaggc atttcttcaa
     2101 aaagagtggg taagggaaga cgcacatatc aatgctgtgg gcacatttac tcctgataca
     2161 agagagataa gtacatcgtt gatggaaact tctcaaatct atgtagatga ttactcagct
     2221 gccttaacag aaagcgggga cctgcttata gccatggcag aaggagattt agaggaatct
     2281 tgtattgttg gtacattagg cgaattagtt tctgagaaaa aaacatttta taaaggagaa
     2341 tcgaggccga cgattttcga cgcagttggt ctagctattg aagatctttg ttgtgcagaa
     2401 tatatttata acaaattaga gaaaggggct ttaaaatgaa cttagattat tacgtagttg
     2461 atgcatttgc agatgaggtg ttcaaaggaa atccagcagc ggtttatgtg ctagaagaat
     2521 ggctgccaga aggtacgatg caaaaaattg ccattgaaaa caatttatct gaaacagcat
     2581 ttacagtgaa gaaaaaccaa gaatttgagc ttagatggtt tacgccagat cgagaaatcg
     2641 atctttgtgg tcacgcgaca ttagcaacag catttgtttt atttaactat tacaaaatac
     2701 ctgatgaaac gattaaattt tctactcaaa gcggtaatct atttgtgaca agaattaaag
     2761 attattatta catggatttt cctagtatta tgccgaaaaa agtgccgata ttgtctgaat
     2821 atgaagaagc gattggggct aagattaaag aagcctactt agcacgtgat ttatttttcg
     2881 tattagaaga tgaaaaaacg gtggctaaat tgcagccaga ttttacagct atcaaaaatt
     2941 ttgaattagg aataggagtc atcgtgacag cagaaagtgt gcaaaaagac tttgtttcta
     3001 gaaccttctt tccaaaatta acaatcaatg aagatccagt atgtggatca gcacattcta
     3061 atcttattcc ttattgggca gaaagaatga ataaaacaaa attgtcagct tatcaaatgt
     3121 catcaagggg cggggatcta gaatgtgaat taaaagaaga tcgtgtgata ataggaggaa
     3181 aagcagtttt gttttctaaa ggtacctcgt ttatttgatt tctttcaaat gaattaaaaa
     3241 taaattggtg gagagtggca accttatagc tacaatggtt gtt
//
LOCUS       DAZULY010000242         3281 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000242 DAZULY010000000
VERSION     DAZULY010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.246"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACG81A_002519"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="ACG81A_002519"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFS5015837.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="ACG81A_002520"
     CDS             complement(2261..2512)
                     /locus_tag="ACG81A_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFS5015838.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..>3281
                     /gene="lexA"
                     /locus_tag="ACG81A_002521"
     CDS             2659..>3281
                     /gene="lexA"
                     /locus_tag="ACG81A_002521"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFS5015839.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          894 a          724 c          613 g         1050 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt a
//
LOCUS       DAZULY010000243         3233 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000243 DAZULY010000000
VERSION     DAZULY010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.247"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1000
                     /locus_tag="ACG81A_002522"
     CDS             <1..1000
                     /locus_tag="ACG81A_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5015840.1"
                     /translation="MKFIKYLDNTGKSPNTIKAYCYHLKLLYEFMEQRGVILNDINFE
                     LLADFVGWLRYPSASNVIDLQSKKAIREETTVNTILNVVMSFLDYLSRLGEFKSIDVF
                     KQAKGRNFKGFLHHVNKGRYQKNVLKLRVKKKQIRTLRSEEVKQIIDACHTKRDKLIL
                     MLMYEGGLRIGEVLSLRLEDIVTWDNQIHLTPRDVNVNEAYIKLRKERTIHVSKELMS
                     LYTDYLIYEYSEELEHDYVFISLKEGYFGKPLKYQSVLDLVRRIVKRTGIEFTSHMLR
                     HTHATQLIREGWDVAFVQKRLGHAHVQTTLNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            997..2889
                     /locus_tag="ACG81A_002523"
     CDS             997..2889
                     /locus_tag="ACG81A_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFS5015841.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            2896..>3233
                     /locus_tag="ACG81A_002524"
     CDS             2896..>3233
                     /locus_tag="ACG81A_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HFS5015842.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQN"
BASE COUNT         1232 a          424 c          641 g          936 t
ORIGIN      
        1 aatgaaattt attaaatact tagataatac tggtaagtct cctaatacca ttaaagcata
       61 ctgctatcat ttaaagttgc tgtacgagtt catggaacag agaggtgtta ttcttaatga
      121 tattaacttt gagttgttag cagacttcgt aggttggttg agatatcctt cagcatcaaa
      181 tgtaattgat cttcagtcaa aaaaagccat aagagaagaa acgacagtga atacaatttt
      241 aaatgtagtt atgagttttc ttgattattt aagtagatta ggagaattta aatcaattga
      301 tgtatttaaa caagcaaagg gaagaaattt caaaggattt ttacatcatg ttaataaggg
      361 tagataccaa aagaatgtct taaagttaag ggttaaaaag aaacagataa gaacattgag
      421 atcagaggaa gttaagcaaa ttattgatgc ttgtcatacg aaaagagata aattaatttt
      481 aatgcttatg tatgagggtg gtttaagaat cggtgaagtg ttatcgctta ggcttgaaga
      541 tattgtcact tgggacaatc aaatccattt aacacctaga gatgttaatg ttaatgaagc
      601 ttatattaaa ttaaggaagg aaagaacaat acatgtgagt aaagaactta tgtcacttta
      661 tacagattac ttgatatatg agtatagtga ggaattggag catgattatg tttttatttc
      721 cttaaaagaa ggctattttg ggaaaccact aaagtaccaa agtgttcttg atctagttag
      781 aagaatagtt aaaaggactg gaatagaatt tacatcacat atgcttcgcc acactcacgc
      841 aacgcagcta attagggaag gatgggatgt tgcgttcgtt caaaagagat taggtcacgc
      901 acatgttcag acaacgttaa atacctatgt tcatctttca gatcaggata tgaaaaatga
      961 gtttaataaa tacctcgaga gaaaggagca taagaaatga atgcttctag taaaaggaaa
     1021 attattagtc agagtgagat tagcaaaaaa atagctgtaa tgaatgaaga aatgcagggg
     1081 ttttgggcta ataatagttg ggatataaga aaatgtccac atccttctgc catagaatta
     1141 agtaagaatc ctgctttaag gaatcgttgg gttcgttttg aacgtgttaa aaatctgtgg
     1201 ttaagaacag aattgaaata tttttatttt taccatttaa acaatggaat atggaatgca
     1261 aaaactgtct ggattagaaa aggaacagta attaataaaa tgttagattt cttagattta
     1321 aagtatccta gcattacttc aattactgaa gttcctattg aaaaagcaat gacggagtat
     1381 agaacttatt tgacaaaacg gggtgttaga attaccacta ctaattataa gattactgct
     1441 aatcaagaaa aaacacctgt aaaagctaat tcctactatg ttactaatct aaaacaattt
     1501 atggagtttt atgagaactt ttattttgat ggagaggagt gggataaaga cgtttgggat
     1561 agacgtaact tacctttgcc agatgataag gttaacccaa cacaatatga atatacaatt
     1621 aactttaaag ggtttcggaa tacatatttt aaacaacttg taaaaagata ttgtaagttg
     1681 agattgaaca tggatagctt ttcctatgta agtgatattg cccaaagact taaagagttc
     1741 tttaattttc tggacatgaa atttaaacaa gttcagagag tacaccaatt aacgagagtg
     1801 gaaattgaag catatttaag tgaactaaac atgatgggaa taaaacctag tacaataact
     1861 gggaggatct ctatattgga aggactattt agtacccttc ttaggctaga atgggatgat
     1921 gttccttcca aaatattaat ttattctgag gactatccga aaataccaag agcaaaacca
     1981 cgctttatag atgaattcgt cctagagcaa ttgaacagtc atcttgataa attacccgaa
     2041 tatatagcta cgatgactat gattgttcaa gaatgtggaa tgaggataag tgaattgtgc
     2101 accttgaaaa aaggctgtct attagaggac aaagatggag atttcttttt aaagtattat
     2161 caatggaaaa tgaaaaagga gcatatagtt ccaatatcta aagaggtagc tttacttatt
     2221 aaagttcggg aagataaagt ttcagaggaa tttccagata gtgaatacct ctttccaaga
     2281 aaagatggat cgccattaaa acaagaaaca tttagaggtg agttaaataa attagcttat
     2341 gagcaaaata tagtggataa atcaggtgag atttatagat tccatgccca tgcctttcgc
     2401 catacagtag gaacaagaat gattaacaac gggatgcccc agcatattgt gcagaaattt
     2461 ttggggcatg aaagcccaga aatgacaagc agatacgctc atatctttga tgaaactcta
     2521 aaaaatgaat ttactaaatt tcaggaaaaa ctggttacca ataatggaga tgtgcttgat
     2581 ctagatgaag ataatgaagt cgatgatgta gagcttcaat ggttcaagaa aaatataaat
     2641 gcacaagtgc ttccaaatgg ttattgtaga ttgccagtag tagcaggtgg ttgtccacat
     2701 gcgaatgcat gcttagattg cactcacttc tgtaccagta agcaattctt accacagcac
     2761 gaagaacagt tagagcgtac agaagagtta ttagccatag ctaaggataa acaatggcaa
     2821 agacaagtag agactaatag ccgtgttaaa gagcgtttag aacaaatcat tggaagtttg
     2881 acggggtaat tatcaatgga taaacaagtt agaaatacaa cagaaattgt acgtttggcg
     2941 aagcagaaat caaaaaagac aagggaaaaa gtagacaaag cgatttctaa attttcgatt
     3001 gaaggtaaag ttattaattt taattcaata gcaaaggaag ctaatgtttc taaatcatgg
     3061 ctttataagg aacacgatat taggcaaaga atcgaatccc ttcgtgagcg tcaaataaca
     3121 gcaaatgtag tctcaaaacc caagaaaagt tctcgttcgg aggaaatcct tattaaaacc
     3181 ttaaaaagaa gagtaatgga attagaaaaa gaaaataaaa aattacagaa cca
//
LOCUS       DAZULY010000244         3221 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000244 DAZULY010000000
VERSION     DAZULY010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.248"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACG81A_002525"
     CDS             301..585
                     /locus_tag="ACG81A_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015843.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACG81A_002526"
     CDS             588..1004
                     /locus_tag="ACG81A_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015844.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACG81A_002527"
     CDS             complement(1141..1860)
                     /locus_tag="ACG81A_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS5015845.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2544..2630
                     /locus_tag="ACG81A_002528"
     CDS             2544..2630
                     /locus_tag="ACG81A_002528"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFS5015846.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..>3221)
                     /locus_tag="ACG81A_002529"
     CDS             complement(2932..>3221)
                     /locus_tag="ACG81A_002529"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015847.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DAZULY010000245         3195 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000245 DAZULY010000000
VERSION     DAZULY010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.249"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..224
                     /locus_tag="ACG81A_002530"
     CDS             12..224
                     /locus_tag="ACG81A_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015848.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(502..2301)
                     /locus_tag="ACG81A_002531"
     CDS             complement(502..2301)
                     /locus_tag="ACG81A_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFS5015849.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2425..3021)
                     /locus_tag="ACG81A_002532"
     CDS             complement(2425..3021)
                     /locus_tag="ACG81A_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFS5015850.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1130 a          589 c          572 g          904 t
ORIGIN      
        1 gagggaatga aatggtaaaa gcagaagtag gaagatctta tatatgcaaa ccgatcggag
       61 tatctcatga actaatcggc tgtattgaac gtacatatat caacacggct gtcgtgtatg
      121 ttgaaagtta tgaccaacga gactatcaac ttatcaatga aagcaagtat cgcatgctcg
      181 taaaattctg tgatattagc gcacctgctg aattggtttc ttaaacagta tttttgagaa
      241 ttcgcaagaa ctcccttata atataaataa agacagaagg gagcgagaag atatgccaaa
      301 aagaaaaagt tcagggaaaa tgggaattat tcttgcagtg atctctgcaa ttcctgctat
      361 tttagctatt gttcgtgcga tgaaggaatc aagagaaagt taagcacaca ttatgagcgt
      421 caaacaaaaa acgcatatac ctgcggtggc aggttatgcg ttttttgttg tataaagact
      481 tttttcatta acgatttttg attagccgac tcccaaaaca aaatttagca gtttatctga
      541 ataagtgatt tcttcatctg tttgtttcac actttcattt agtagtccca tctcatcggt
      601 tatgatacca tccacaccat aaaacatcat gcgactcata gcatctgaat cgttgattgt
      661 ccatacatat acttcttttc cgtcttggtg ggcagaatta atgaagttac ggttgatcgt
      721 actatattcc attgtcagaa aatcagcagg cgtcacaggg ggcccaacaa tattgaaagg
      781 taagatgtag cccacataaa agtttggttc tgcttctttt aaatctgaaa cggtttggta
      841 cgtcaaactt tgcaaaatat gatggttctc taagataact ttgcggtatt tatggacaaa
      901 tcgttcgaca aggtcatccg aatctttttt agaggtcttg atctcaataa gtaatttttg
      961 gtttagctta tgtgcttttt ccaaataaga atcaaaagaa caaacagggg cttgcactcc
     1021 gttttcagta actgtaagat ttgtcaattc tttcaatgta agatcctgtg gtgttccatt
     1081 tacccctgtt aagtgacgaa gatttgcatc atgcatcact acgaaatgac catcttttgt
     1141 ttcctgaaca tccatctcta cataatcagg atgatagtct ctgcttgttt tttcaagtgc
     1201 ttctaaagta ttttgcgccc cattttgggt actcacacct ctgtgagaga cagtcactgg
     1261 ttttgtctga acagctgaag tcagataatc catattgtaa agacagacgc caataccgaa
     1321 aaaaactgca aaaagagtca aaccagtgtt tttcagcgca gagaaacgca agttgggggc
     1381 ttgtgggata aaccacttag ggatctcagg caagaagcct tcgtcatcca tgaaatcaac
     1441 aatgatataa aaaatcccta cggtagacat cacgatattg aataataaaa tcccttgtaa
     1501 aagcgtcata gcaaacacag cgctgatcaa tgcatagtct gggaaaaatt gttcaaccat
     1561 actttgtgcc aaaataacaa cgatatatcc taaagaagaa aggagtaaaa tcgtaccgcc
     1621 aatcacaata aattggcccg tgatggccaa aagacgcgat ttggtcaatg accagctttc
     1681 ccgaatggct gctcgaaaag gacgatctcg tagaatcatt tccggtaaag caaaaatcag
     1741 tcgaatccca atatagccaa ggaaaatata aacgagtaaa aaggaactga cgatgatcca
     1801 acgattagca aaaataaaat ccataatgaa agcggggatt tttatttttg acagtagatc
     1861 agaattaaaa cttaatccgc tgatcggtag aatcaataaa aaataagcaa ggaaaaacaa
     1921 aaaagtaatg ggtctcactt tcttcagctg aagtatcgtc aagtgtaaga gttgcttcaa
     1981 agagattggt tctttctttt taataaaaaa aacactcatc aaaagaaaag taaattcgaa
     2041 ataaactaac agtaaaatcg tcatcaaaac tacgattaac gagagtagaa ccccaggatg
     2101 ttgagaaaaa attgaaggaa tattgtcata agacaaatag tcaattgccc ctcggtgtaa
     2161 aataaaacgg gtcgtactgc tcaataaggg taaacaaata aataaaataa agccatgcat
     2221 cagcaagaca tcacggaaat aagcttgtgt tccttttaaa aagtcccaag cattacctaa
     2281 gctacgtttc aaataattca tcctatcacc tgctcttcac attgtagcgc aaatctaatg
     2341 aaaaaggaaa gggtgcagtg tgttttgcag accctttccc agatttaaca ttgtcaatca
     2401 atgactaatt ttacaaaata aatattatcc aagaagacca cttagatatt gacctccata
     2461 aatcaaggcc atactatata agaaaatcga cagtggttta gcaatcaaaa tgattagtgt
     2521 atatcgtttc actgacatgt ttgtcaatcc agccatcaaa cataacgcat cgtcaggagc
     2581 aactggaaga aaaatagcta gagcaaacag cttctcaaaa cggttttgat tatccagcca
     2641 gccaatatat ttgttatatg tcttatcgct aatcatggaa agtatgaatg gctttccata
     2701 acgacgccct agcagaaaaa tgataatcga accaatgcag attccaatat agttatagat
     2761 aaatccagca taagggccaa atatcaggac accagctgca gtactgatcc ctccaggaat
     2821 gattggaata actacttgca ggatttggat gaagacaaaa ataatcggtc ctaaaatgat
     2881 ggaatcacct actaatcctc ttaacgcatt caaatctttg aaaacaccta aattaataaa
     2941 ataaatcgtc agcgcgattg ataggcctaa tccgataatg gatatgaaat ttatcagttt
     3001 tcgagaggca gcaatattca ttattcatca actccttaga ggttctttgt taactatact
     3061 ttactagaaa agcaactaac tgcgccattt tttgatatga cagaaaacaa acaattttgt
     3121 aagttcatca taagtataaa agattgatct caaaaaaaca tccaactaaa gacttattta
     3181 ataacttgtt ttttt
//
LOCUS       DAZULY010000246         3184 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000246 DAZULY010000000
VERSION     DAZULY010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.250"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..674
                     /locus_tag="ACG81A_002533"
     CDS             105..674
                     /locus_tag="ACG81A_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5015851.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(852..1292)
                     /locus_tag="ACG81A_002534"
     CDS             complement(852..1292)
                     /locus_tag="ACG81A_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFS5015852.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            1450..2214
                     /gene="map"
                     /locus_tag="ACG81A_002535"
     CDS             1450..2214
                     /gene="map"
                     /locus_tag="ACG81A_002535"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFS5015853.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            2246..3169
                     /locus_tag="ACG81A_002536"
     CDS             2246..3169
                     /locus_tag="ACG81A_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFS5015854.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
BASE COUNT         1016 a          549 c          644 g          975 t
ORIGIN      
        1 ttgtgaagaa aatcaaaaac taattttgca tatcaaaaaa acatgttaca atgaaattaa
       61 tcaatggtgg aagcgttata aaaaaattaa ggagattaaa aaatatggca agaaaaaaga
      121 cgattactag agaacagatt ttggcagctg cttatgaagt agctgccacc gatggattta
      181 cacgatttac tgcgagaaac atcgcgaaca aaatgaattg ttccacgcaa ccgatttatc
      241 ttgagtttaa aaatatggat gacttgagac aagctctttt tgagcagatc tatgatcatt
      301 tacaatatga agtgttcact gttgagcata cagggaatac gattatcgat cttgctttta
      361 attatatcca ttttgcaaga agagagaaga agttgtatcg ttcactgtac ttagaagaat
      421 atggtggagg aaaagagatg cagaatttct cttatactta ctttgtgaat acagtgaaac
      481 gcgatccagc ttatgtgaat ctttcagaag agcagatcga atctttgtat aatgggacat
      541 ggatcatagc tactgggatc gcagctttga tgtcttcaag catcatccat ccaactgatg
      601 aacagattga aaagatgatc caagacagta tagatgctat cttgaaattc aaagaaccaa
      661 tcgaaatcga ttgaatatac tcaaaagttc cacaaatcag taggtaacaa gagatgtgtc
      721 aaaataagga aaagttcgtt tttcttacag cgtatgcatt tttaactccc gaagaactct
      781 tccatgaatg caatgaataa aagagccata cagattgaat aaatctggat ggctctttta
      841 ttgcaagtat attaaatttt tgcgactagt tctttagcga aagcttctaa attttcgata
      901 tcttcttctt cagcattcag atccacttta acattttcgg ctccttttga tgcgcctgtt
      961 tttgcaaaag cagcatcgaa atcatccacc gatttgcaga attcatcgta aaaagtatca
     1021 ccagatccgc agacaccgta aatttttccc aatagatcca attcttgcaa gtcttcataa
     1081 aagtctacga tctcatcagg cagatcgcca tcatcgtatg tataagtagc tactacgcaa
     1141 atatctgcct cctcgaattc atctgcatct acttgggtac attcgttgat ttcgacttcg
     1201 atatccagat tttcaaatgc ttctgcaaca atatcggcga tttcttcagt gtttcctgtc
     1261 atgctagcat atacgatttt tgctaaagcc atgatgtgcc tcctcgttta taattattta
     1321 acaattatga attataccaa aacatcattg aaaagaaaat aaaacctaaa gacacctggg
     1381 gaatgtcatt tggctaaatg tatgccgcta tgttaaaatg agagggattt gtaaaaagga
     1441 gacgaaaata tgattacttt aaaatcagca cgtgaaattg aaatgatggc agaatctgga
     1501 gctttattag cagatgtaca taaaaatctg cgtgatttta taaaaccagg aatcacaagt
     1561 tgggatattg aggtatttgt cagaaactat atcgagagtc atggcggaat tgctgcgcaa
     1621 attggctttg aagggtatga atatgcgact tgtatcagta taaatgatga aatttgtcat
     1681 ggattccctc gtaaaaagcc tttgaaaaac ggtgatttgg taaaagtcga tatgtgtatc
     1741 gatttgaaag gcggcatttc tgattcttgt tgggcctatg tagttggaga atctacacct
     1801 gagatcgacc gattgatgga agtaacgaag aaagctttgt acatcggtat tgagcaagca
     1861 caagtaggga atcggattgg tgacatcggt catgctatcc aaacttatgt agaaggcgaa
     1921 aatctagcga tcgttcgtga ttttattggt catggcgttg gtcctacgat tcatgaagaa
     1981 ccagcagttc ctcattacgg agaagctgga aaagggttgc ggttgaaaga aggcatggtt
     2041 atcacgatcg aacctatggt taataccgga acttggaaaa tgaaaatgga tcctaacggc
     2101 tggactgcgt atacaagaga cggcggactt tcttgccaat atgaacatac attggctatt
     2161 actaaagatg gtccaagaat tttgacatca caaggagaag aaggcactta ttgatctatc
     2221 cagtcaatag gtacgggaga ggtacatgaa agtttggaac aaaataaaac aaaatgaaaa
     2281 tctgatgcgc tttattgaga cgactcaaaa gcgcatcact gattcagaaa tgagtacgac
     2341 ctctgtcgtc gttgcttatt atttactctt gtctttattt ccattgatta ttgcttttgg
     2401 taatatttta ccgtttctcc acatcgatca agagactgta ctgacctatt tacgagaagt
     2461 gattcctgag accgtttacc agtttatcgg tcctgcaatc aaagaccttt tgacacaaag
     2521 ttcaggaggc ttgttatccg tttctgcatt agcggcttta tggtctgcaa gtcaaagtat
     2581 caatgctttg caaattgcga tgaataaagc gtatggcgta gaaaatagaa aaaatttttt
     2641 gatcgtccgt ttcttctcac taattgtcat tgtcttgttc atgattgcta ttagtggggt
     2701 aactatcgtt cttgggttgg gacagctgat tttggatgct attcagccaa tcgtacaaat
     2761 acccgtgaac tttatcgatc agttccaaac tttgaagtgg ccaatcacat tggtagcttt
     2821 atttgtcatc atgttcttga tttatttaat tgtccctaat gctcagttga agttgaagac
     2881 gatcattccg ggcacgatct tcgctactgt cggctggatg ctgttatcac aagtgtttag
     2941 tatctatgct aggtattttg caacgaggat cagcggttat cagatcatag gaagttttat
     3001 tgtactcatg ctttggctta attttgctgc tacgatcatc attttgggcg gaatcatcaa
     3061 tgcagttgtt caagaatacg tgacgggtca tcaaatcaaa aaacgtcgag atccaagccg
     3121 aaaatggttc aataaattga aaactaaatt ttctcataaa aaaaagtaga aacaagagtt
     3181 ctta
//
LOCUS       DAZULY010000247         3129 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000247 DAZULY010000000
VERSION     DAZULY010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.251"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            103..1347
                     /locus_tag="ACG81A_002537"
                     /pseudo
     CDS             103..1347
                     /locus_tag="ACG81A_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="ACG81A_002538"
     CDS             1360..1992
                     /locus_tag="ACG81A_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5015855.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2200..2424
                     /locus_tag="ACG81A_002539"
     CDS             2200..2424
                     /locus_tag="ACG81A_002539"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFS5015856.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2526..2759
                     /gene="relB"
                     /locus_tag="ACG81A_002540"
     CDS             2526..2759
                     /gene="relB"
                     /locus_tag="ACG81A_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFS5015857.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="ACG81A_002541"
     CDS             2749..3018
                     /locus_tag="ACG81A_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS5015858.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1102 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttta
//
LOCUS       DAZULY010000248         3066 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000248 DAZULY010000000
VERSION     DAZULY010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.252"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            208..444
                     /locus_tag="ACG81A_002542"
     CDS             208..444
                     /locus_tag="ACG81A_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015859.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            451..2403
                     /locus_tag="ACG81A_002543"
     CDS             451..2403
                     /locus_tag="ACG81A_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS5015860.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2475..2972)
                     /gene="dfrG"
                     /locus_tag="ACG81A_002544"
     CDS             complement(2475..2972)
                     /gene="dfrG"
                     /locus_tag="ACG81A_002544"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS5015861.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1081 a          513 c          543 g          929 t
ORIGIN      
        1 gaaaatgtaa acctaaatga gtatcaaatt gcaactgaat ttgatactcg ttttttattg
       61 ttaaatcaac gtttgttgtt aatttacacc acaaaatgta agttaaaatg tatataaaaa
      121 tgcgaacgtg aaataattat tgtaggaatg caaattggtg tgttaaaatg gttacaattt
      181 actaagcaaa aaggggtgca ttttcctatg caatttcttg agccaaaaaa taagaacgct
      241 aagtctgtgg attgggaaat atcagagcaa gtaagggtga tagttaagca atacgctgaa
      301 tatgcagagc gtactgaaag cgaagcagtg gatgaattct tattgaacat tttagacgat
      361 aagaagttta ttgaatggat tgccaacaag cgaagtaata agcggattgt tgaaaaaatg
      421 ggtatcaaag atagagtggg ttgatagaca atgaccaagc ttaaaagatt agccacgaag
      481 gaagatgaaa ttgtagatgt taaaattccg atttcaaacg aagaattaaa agatagggct
      541 aaacaatatg atttattaac tccaaaacga tttgcaacaa ggtataacaa aatgttgttc
      601 ttgccaacgt cttttaaatg gaacggttct gaatatccca ttcaatataa ttactgcatc
      661 aatccgtttt gttgtaattt tgggaaagaa cagcacaaat tcaaagatgt aaaagggaaa
      721 ccaagtcgat ataaaatgac tggtagttcc aaagataaag gtcataaagg tatgtactgc
      781 aatgacaatc ctattggcag aggtgtatcg caaaactgta cagtaacccc tttatctaat
      841 tggtctgttg ttgaagaaat taaacgcctt atagaaatca atagcatcca agatgttgag
      901 ccagattatc aatttcataa agaaggctgc tcggaagaag aatcaacccc attcaatgag
      961 ccgaagcaat tttacaaaag ggggaaaagt aggggcaaat ctcaacgcta tcaatgtaag
     1021 gcgtgtaaga agttcactaa tgttctacca aaaagagaag aaaccactac ttatcaccaa
     1081 caaaagaaca ccattcttcc aatgttcgct aagatggttg ttggaagggt ttcagtcagt
     1141 cgtacttgtg atattttagg aattggagtt ggaacctact atcataagct ggagtggctg
     1201 tataggcgtt gtttggagtt tttagaacga tatgagacac aaccccttca aacaaagaaa
     1261 tttaacgaga tgtggcttaa tacggacaag atgcactact acctaaataa cgtccgtaag
     1321 aaaggacaag gttcaaagaa atatacgggg tttgaagacc ttaatatgca aacgtatata
     1381 gttgtatctg ctgaggtctt atcaagatat gtgttccgtt ctgatgttgc ctatgattgg
     1441 aatatttcaa tggatgaatt aaatgaagat acaagaaaat tcaaagaaga ccatctcaat
     1501 acttttagtc gtaaaaatga taggttagat tggtcttatt atccacaaga accatccgct
     1561 aacgatagcg aaaataggaa tgcttatctt cacgaattag ggaaaattac taataggagt
     1621 agatttgtcg atggtttaaa tgttgatgct ccttatacta caactgctca ctattggtta
     1681 attaaacaaa tggttaatgc cgacgaatgg cgaatgatta gtgatgatga cttctctatt
     1741 agaaacgcct tttatagagt attcacgaaa gaattaaggt tgagcgatgc acatcatttt
     1801 atttgccaag ttaacaagac gaaaagcaga aaacaatgct taaaagaatt cggacaagca
     1861 aaagcagaac tgctcgattg gggagatatc agaggattca aaacaaagtt tctacggact
     1921 ttggcatctc attatctaac tgaattacta acaagtcatc aattccacga agaggctatt
     1981 agtaaagatg gagaaagata tcgcaaatat gccgataacc ctatcaaaca tccattagcc
     2041 actaaggata aaggatttta ttcagttgat tgtagaacgg atttatctgc tctggaacca
     2101 aacgaaatcg caaagatgct attaaatgtt aatgaccatt cgaccaatag ctttattcaa
     2161 cagataagac gttatatatc atctcttgaa agaccattaa caacagctcg tggtgataag
     2221 aagagctata tttacgctaa cttcaatccg aagtatgctc aatttgcgat tacaattcta
     2281 agaacttact acaacttttg tcgccctttt aaatcagcgg ataaaaaggt tttgacacct
     2341 gctcaaagat tagggattac agataaacaa tttgattgga aagatataat ttatttcaaa
     2401 tgacaaaaag agcttggagt aatcccaagc tcttgtgctt tatctttact gttcttcttc
     2461 aattaacgca cccgttaact caataagttt tttctttcat atacatgaaa atagtagtta
     2521 tacggatttt tatcattctt tatccctttt tgggcaaata cctcattcca ttcctcataa
     2581 ttcacttctg gaaaaaaagt atctccttcg aattcatgat gtatttttgt gatgtacatt
     2641 ttctcaacat aagggaaaaa caaattataa atctgttctc ctccgaaaat aaaaatttct
     2701 tcttcgtttt tacataactc aaaaacatct tctattgaat gaacaatttc acaaccatta
     2761 aaggtaaacc ccttatctct cgtcagaata atatttcttc tgtcaggtaa ggctcttccg
     2821 attgattcaa ggttcttcct acctaatatt atcggatgtc cctttgtagt atttttaaca
     2881 tattcccagt ccttgggaat cctccaagga atgtcattct ctttgccaat cactctattc
     2941 ttatccatcg cagcaatcaa agaaactttc atttataaca cagccttttt aactaattta
     3001 ccatctaatt tcttatttct atcaaaaaac cttttctcat tatcctgccc gttagttgaa
     3061 taagca
//
LOCUS       DAZULY010000249         3052 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000249 DAZULY010000000
VERSION     DAZULY010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.253"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..352)
                     /locus_tag="ACG81A_002545"
                     /pseudo
     CDS             complement(1..352)
                     /locus_tag="ACG81A_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(612..1160)
                     /locus_tag="ACG81A_002546"
     CDS             complement(612..1160)
                     /locus_tag="ACG81A_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015862.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1161..2018)
                     /locus_tag="ACG81A_002547"
     CDS             complement(1161..2018)
                     /locus_tag="ACG81A_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS5015863.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2304..2590)
                     /locus_tag="ACG81A_002548"
                     /pseudo
     CDS             complement(2304..2590)
                     /locus_tag="ACG81A_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2580..2909)
                     /locus_tag="ACG81A_002549"
     CDS             complement(2580..2909)
                     /locus_tag="ACG81A_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5015864.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2986..>3052)
                     /locus_tag="ACG81A_002550"
     CDS             complement(2986..>3052)
                     /locus_tag="ACG81A_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS5015865.1"
                     /translation="KVDEDEHVVEIYSARTHYESQ"
BASE COUNT          892 a          620 c          384 g         1156 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac ttatttgaga gtcatctaat tggctcataa
      241 tcctttcttt ttggttagta tctgcagtta cttgtttagt atgagccatg aacccaaaca
      301 tactcacccc aacaaccaat cctaaatata ttttcttttt aaactttttc atctacatct
      361 cctcctaata gttttatttt agagttagct ctattactta tttactaact caacaacaga
      421 ttatcataca cttgttaatg aaatcacaat atctcattaa aacacaccct aaaatcataa
      481 ccgttcctat gctcattttt tggatttttt aatcacgtaa ccagaaaaat aaatcccctc
      541 tatttcacgc aaatcaaaga agaactaagt aactaccgtt taaaggtagt tacttagttc
      601 ttctttgatt attatctaac tttattattc attccccacc ctttgcgcag tgagtcttcg
      661 gtatcttgtt ttatctgact ggtagttctt attatttctc cacgatcatc taattctgga
      721 acaattggat atttttctct gaacaagtct agttctttca ccacgtcatt aaacaaatct
      781 tgcttatctt cttgcgtaag attctgttta atatactcat ctaaagcttt tctaagcaat
      841 tcatctcttg tcatattagt ctcatacttt tctactaaaa ctctggaagt cgcatttaac
      901 aaagtcgaca gttttaaagg atgcttggtg gaataaacct tatcaaactt tctgattggt
      961 gcgccaactt tttttgttct tcctctactc tgtctttcaa cattggagga agtttttatt
     1021 tcttgaactt ggtgcacttc taattgctta ttttcagatg gtgtaattgc ttgctgcttt
     1081 ttctttgctt ctgcttcttt ttttattttg gcgagtcctt tgttgttccc aaaaatatca
     1141 agtcctttga tgtcagccaa ttactccacc tccgcatacc aattctcgtg ttcttccaat
     1201 tctttcaaaa tactgataaa taattcgtgg cttttgcgat cccattgttt taagagacct
     1261 ttctttgtct tctccatcgt aatacctgat ccgtcgtatc gttttaagcg cgcatttttg
     1321 agtacaacat ttttaatcaa atgttcccca taaatctctt gggcttgttt atacatttcc
     1381 tgatctactg cgtcaccgct ttccaacatg aaaggaataa caccagcaac atataaattt
     1441 gtatcaaatt ctttcacaaa aaagtccata tactcaagat attgcatcgc atttctcatc
     1501 gattttactt gggtctgtaa gacgacgatt gcccaatccg caatgtacat cgcggattta
     1561 ctatatacag aaatagaagg tgggacatct aaatagatga cgtcaaactc atcaaataat
     1621 ggttctagct tttctttcat atatttgaat tgggataatg aatcattata ttggtctttg
     1681 atccaatcat catacatttc aaaggacatg tctgctggaa gtatatagag gttattgatt
     1741 actttataga cagcctcttt aggtggaaga ttgtttatca acacgtcagt cacagtattt
     1801 tcgtaaacaa tcggttcacc ttctccgcct ccttcttcgt tagtgaagtt catatgcgtt
     1861 aagttcatca catcagaagt atttccttgc atatccaagt caattatcaa ggttcttttt
     1921 ccttcattaa ctgctgattc aaaccctaac atttgcgcgc acgtactttt acctacgcca
     1981 cctttaaaat taccgattac atatttcttc gctaccacgt tcctacctct tttcttttag
     2041 caatattcat ttgatactat tataacatat ttatttttag ttactaccaa aaaaaagata
     2101 tatatgtact accttataat taataagtaa aaggtagtac ataaataagg aaaagctgaa
     2161 atatattgat ttatagcttt cctcaaagta atgacgttat ttaatttatc gagaagcaga
     2221 gaatactcgc gactttagtc atgtagacga atcagatttt cgataaaaaa gaagccgtcc
     2281 ttcttaagaa cgacaacaaa tcactagatt aattttttca actgttcata aaagttttca
     2341 tgcgttccac ataacaatac tggaataacc ttgttttcta taatctcgta agctactcta
     2401 tatgttgtgc ccgcatattt gaacccacta gaccaaatac ctgataaatc cccagttttt
     2461 tgttcaccct tatatggatc aatcgcaatt tcatcataaa ttaaattcaa aaatttttct
     2521 ttcaatggct tctcttttag ctttttaaat acttaatgac atgttttgaa ggaacgattt
     2581 cataagccaa tttctgttgc caactcctct ttagtcatta ctttagaatt agtaagtgta
     2641 tcttcagcaa tatctctaaa ggaagaatgc attttgtttt ttctattctt aaattctaca
     2701 agaatctcat ctttatcgta gccttctgca attatatcag acaaaatatc ttcgctaaaa
     2761 tcataaaatt ctttttgtgg ttcaacaaat tcataaagaa taccattctc taccaacttt
     2821 gcttcaactt cgactccatt aggaacatta aaatctttag gtactgtcaa catgattgaa
     2881 ttgccttgtt ttcttgtttt tgtaatcatt taaatccctc atttctaaca gtatgtaatt
     2941 acttagtaaa tacattatac cacgagataa tgtatttaca agtaattact gtgactcgta
     3001 atgcgtcctt gctgaataaa tttcaaccac gtgttcatct tcatctacct ta
//
LOCUS       DAZULY010000250         3043 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000250 DAZULY010000000
VERSION     DAZULY010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.254"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1174
                     /locus_tag="ACG81A_002551"
     CDS             <1..1174
                     /locus_tag="ACG81A_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HFS5015866.1"
                     /translation="EILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDYEKYDRI
                     GFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEETVIHLF
                     RKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAIYMDATY
                     IPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERGLKNVLL
                     FITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSK
                     EVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIESFNKQI
                     KKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQLIEN"
     gene            1308..>3043
                     /locus_tag="ACG81A_002552"
     CDS             1308..>3043
                     /locus_tag="ACG81A_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFS5015867.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILT"
BASE COUNT         1052 a          503 c          570 g          918 t
ORIGIN      
        1 agaaattcta aagactctag cgaacaaagg cgatttgaat gaattattcc gtgtccattt
       61 ggagaaagca gtcaatacgc ttctcaaaac ggagttaacg gctttcctag attacgaaaa
      121 gtatgatcgc attggtttta acacgggtaa ttctcgtaac ggctcctatg accgtacggt
      181 caagaccgag tatggggaac ttcatctcca gattccgcgc gaccgcaatg gtgagttcaa
      241 gcaacagact gttcctgctt atagacggac gaatgacacg ttagaggaga ccgtcattca
      301 cctcttccga aaaggtatta ccatgtcgga aatcgcagac ttgattgaga aaatgtatgg
      361 gcattactac acgccccaaa ccatgtccaa tataacaaaa tcatttacag aagaggtaac
      421 ggcgtttaat gggcgggagc ttcatgaccg ttatgctgct atttatatgg acgcaacgta
      481 tattccgtta aagcggaaaa ccgtcgccaa ggaagctatt catatcgcag ttggcattcg
      541 cccggacgga tcaaaggaag tattgagcta tgcgattgca ccgactgaat ccatcacgat
      601 ttgggaggaa attttattgg accttcaaga gcgcggtttg aaaaatgtcc tcctgttcat
      661 cacggatggc ttaaagggga tggtaggagc gattagtcgg ttctatccca aagctcgttt
      721 tcaacattgt tgtgtacacg tttcccgtaa tatcagtcat aaagtgcgtg tcgatgatcg
      781 taaggaagtc tgtgatgatt ttaaaatggt gtatcaagcc tcatctaaag aggtggcgtt
      841 ggaagcacgt ggtacttttg cggaaaaatg gaaaaccagc tatccaaaag tggttgaatc
      901 gattctttcg aacgatcact tgctcacttt ctatgatttc cccttggcca tacgcaagag
      961 tatttattct acgaacttga ttgaatcctt taataaacaa atcaagaaat acagccaccg
     1021 caaggaacag ttccaaaatg aagagtcgat ggaacgtttc ttagtctcgt cttttgatac
     1081 ttacaaccaa aaattcctag gtcgcagtca taaaggtttc caacaggccg aaggcgagct
     1141 cgaacaaatg ctaagccaac tgattgagaa ttagaaacaa tctgcagtag ggaagaatca
     1201 tttacacaaa attattgacg ctcccttata atgagaactt ttactaacat aatttatgat
     1261 aaacttacta tataatcata ttaattaaat agttaggaga tctgcttatg tttatatcta
     1321 ctcttcaaat tcgaaatttt agaaattatg aatccgaaat ctttcgattc gataatgaga
     1381 caaatactat tataggtgag aatgattcag ggaaaaccaa tgctcttact gcattgagaa
     1441 tcttattaga tgatagttat tactatagta gtaaaacact aaaagaatca gatttttttc
     1501 atggaattca aaatgggtgg caagggcatt ggattattat ctctgcgaca tttgaaggaa
     1561 tcagcgaaga agaatttgac aatgagattt gtgcttccat ttctttaaat tctgaaagtc
     1621 aaacaatctt agaagaattg atttcaaatg cagataaagg tgtaggtagt atttctctat
     1681 ttattagacc taacaaagct attagaaagc aattatttga tatttccact gaattagact
     1741 ctcatcaatt taacgaattt agaaattcta ttcgtctatc cgactatgaa ttttactaca
     1801 catctaaatc aaatctaaat ttctgtatca atgaaaatta tgataaattg gtaggaagac
     1861 tcaatgacaa tatagctagt aatcctgaag aagacgatga agctctgttg ggctcaagaa
     1921 ttgacatgtc agatattttc aagcatattt cggttgttta tgttgacgct cttcgagatg
     1981 ttcttagaga aatgaaaaac aatcgaaacc cagtcaaaag aatcatggaa acaattgaat
     2041 caaaaatatc atcagataat gttgagtcat taaaaacaat tatacagcaa ttaaatgaga
     2101 ctattaccag tgttcctgaa attaggaaaa ttggcgaaaa tattaatcgg caactaaata
     2161 gcataattgg ttcagtgtat tctcctaatc ttcttctttc ctcaactatg tcagatgata
     2221 tgggttcttt agcaaaattt ctttcaatga agccagaaca aaatatcgac ttagacttac
     2281 tagggttagg acatctaaat atgatttatt tagctttgaa aatagtcgaa tttgaagcgt
     2341 gtcgttcaag agaactactt aatattatgt tgattgaaga accagaagct catattcatc
     2401 atcatattca aaaaactctg ttcgaaggat taaatcttca aaaaaactat actcagattc
     2461 tcatgaccac acattctgta catttagctg aagtgtctga aatatctcgg atgaatgtat
     2521 taaaatcgtt taatggcaaa tctatcgtta tgaatccagc aaataatctg aactcatttg
     2581 ctaaagaaaa gttaaaaaag aacaatctaa atctaataaa cgcagtagag agatatttag
     2641 atgttaaaag aaatggattg cttttttcta aaggtgttat cttagttgaa ggagatgctg
     2701 aagaaatact tattcctcaa atagtaaaaa aattatttgg tataaactta gacgaacttg
     2761 gaataggttt gattaatgtt ggtagtactt cgtttgaata tatcgctagc ttgtttgacg
     2821 atgatagaat acagagaaag tgtgctattg taactgattt agatgaacag gcaatcccat
     2881 cagaacatcg attgtataaa agcactgcac aagagaaagg tcaagcaaga aaagaaaaat
     2941 tatcctccct ttttaatgaa aacaattggg ttaattgttt ttatgctaat acaacatttg
     3001 aaattgagtt tttagatgtg aataaagaaa atattctaac tta
//
LOCUS       DAZULY010000251         3036 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000251 DAZULY010000000
VERSION     DAZULY010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.255"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..383
                     /locus_tag="ACG81A_002553"
     CDS             <1..383
                     /locus_tag="ACG81A_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015868.1"
                     /translation="PFITLNKNSILKEFNNTDYIAFLKLFSVCVLISCVIIFIVAKTC
                     WTMLNKNQRDVENIAIFLIKITSSISIIIPMTVTFSKEQFNVAATYLSFIALFSFVLP
                     KRNKSNFDNKELDEVRNSSPKEQK"
     gene            complement(389..571)
                     /locus_tag="ACG81A_002554"
     CDS             complement(389..571)
                     /locus_tag="ACG81A_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015869.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(571..870)
                     /locus_tag="ACG81A_002555"
     CDS             complement(571..870)
                     /locus_tag="ACG81A_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015870.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(975..1178)
                     /locus_tag="ACG81A_002556"
     CDS             complement(975..1178)
                     /locus_tag="ACG81A_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5015871.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(1193..1939)
                     /locus_tag="ACG81A_002557"
     CDS             complement(1193..1939)
                     /locus_tag="ACG81A_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFS5015872.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            2017..2325
                     /locus_tag="ACG81A_002558"
     CDS             2017..2325
                     /locus_tag="ACG81A_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015873.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(2296..2553)
                     /locus_tag="ACG81A_002559"
     CDS             complement(2296..2553)
                     /locus_tag="ACG81A_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HFS5015874.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(2851..2991)
                     /locus_tag="ACG81A_002560"
     CDS             complement(2851..2991)
                     /locus_tag="ACG81A_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015875.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
BASE COUNT          841 a          584 c          502 g         1109 t
ORIGIN      
        1 ttccttttat tacattgaat aaaaatagta ttttgaagga gttcaataac actgactaca
       61 tcgcatttct aaaacttttt tcagtctgtg tgctaatatc atgtgtaatt atttttattg
      121 ttgctaaaac atgttggaca atgttaaata aaaaccaacg cgatgttgaa aatattgcta
      181 tatttttaat taagattact tcctcaatat caattatcat tccgatgact gtaacttttt
      241 caaaagaaca gtttaatgta gcggctactt atctttcatt tatagcgcta ttttcttttg
      301 ttcttcctaa aagaaataag agcaactttg acaacaagga gctggatgaa gtacgcaata
      361 gcagcccaaa ggaacaaaaa taaaaaaatc atattttagt cagtccctcc cgactggctt
      421 ttttcgctct gtgctcagct tcatcaagcc ccataaaaat ccaaaccatg taaacgattg
      481 tccctatcaa cgcttgtctg cttccccaga ggcctaaagc gtagatgatt agcggtgcgc
      541 tgaatactag tgctctgttg aatttgccca ttaacttgcc tccttcaaaa catctgaagg
      601 cgataaactt tttacacgat atttgtttgc gtctttccac tttaaaaacc aaataaaagt
      661 atggtaatga ataaatgtgg ttgagtgacc tggacgaagt attccttctg caaattcagg
      721 aattgtttcc atttccttgc agtattcaac taaagtagtt tttgacatgc cgtggaactt
      781 ctccaagatt aaactttgtc ggtaccagtc gtctggtttt actgccttct cagcagcttc
      841 tatcagctct gaaagtgttg gttttttcac ttattatccc tcctaaaaat tatcgttcca
      901 tactcgatgt ttaacatttc ttgaagctct gattttgtaa gaatgatttt atcttccaac
      961 gactttacct ccctctacaa ttcgtacata gtgataatcg aatctataat tctgtttgct
     1021 tctgcagaag tctttttacc gtttaaaatt aaagataagt agcttttgct aattccaaat
     1081 ctttcagcaa gcatggtgta agttaagaac tttgaacttt cgacatattc tttgattttt
     1141 tctctatctc gttgagtgat ttctgcaatg tcagtcatac taaaactcct ttctaaccaa
     1201 tttcctctaa atccatttga gggtaatagc cctctgcgat taataagttg taaataaaca
     1261 ctcgcccttt ctgtgtccat ttggtattca ttacaacttt agtgccgcca tcggctttcg
     1321 gtatctcact tgtgtgcgat tttgtgtatc cttggttcat atgttttcgg taaagtatcc
     1381 attgtccgct aactttatgc tgaacaccta actcgtttag caatttgttt agagcaattg
     1441 ccgacattcc gtaatcagct gcaatttgag aagtagctac cgtatctgtc gatgaaagaa
     1501 tcgtatctaa gtagctaatc tttggttcgt actcggcaat ttgttgttcg agtaattggt
     1561 tcttttcttc taaatccgct gctaaacgca atgcttgtgc aaaactttga ggtacgtttg
     1621 aatagctacc tgtttttcta attgttggaa ggacttcact cgttacccaa cgtttaaatt
     1681 ttttggcaga gggaagtttt gattttaaga ttaaactgta aaggcctgac tcgttaatca
     1741 aaacaactgc tgttccattg acggtgaacg aatcgttctg cgtcttatcc tccaagtcca
     1801 catgatctcg aatagctttt tgcggattcg aatatcctag aacgtctgct acatcttttc
     1861 ctacaaaata tggttcatca tttactaaaa tagttcgaac ttcgttttgc tcgaaattaa
     1921 aaatttgtgg tgtattcata tttctcattc ctttctttgg tataatttcc ttatcagtgt
     1981 gacaggctga aataattgtt aaggaggtga aaatttatga aaccagaatt tgtaattact
     2041 atgcttgatg gagaaaaaat caaaatattt gccgatactt tggtcgttgg atacgatgat
     2101 gcgtcaaata tcgaagaaaa agatggaatg ttctattctt ctcaaattta cgtgaatacc
     2161 ttgcaaggtg atattggggt atctactctt gcaacatcaa gaccgcttgt aggtcttatc
     2221 ggctttttgt caaaagcaac attcttttct ctcggcgatg atatagattc ggatgaaact
     2281 gtcatctata aaacatcagc tgttaaaagt atcggtttca aatagtaatt cttgctcgtt
     2341 gatagcggaa gccaactttt cttccaacac atcggcgagc tttctttttg ttggattcat
     2401 ttcaaagttg atagtcttga gaagcatttt tgcatcctta actttttgtc cttgcaattc
     2461 cttgatgatt ttatcggctt ggctatcaat tccatgtgtt agatcatcta tcattttttg
     2521 taccttgatt gtttggctca tttccttgtt cattgtttag cctcctttaa taaatgattt
     2581 tttgtatttt ttaatttcca taaattcctc aaattgctct ttggtattat taccacgacc
     2641 atacatattg tgaaattctt tatggcaact gacacaaagt gtaacgccgt ttgaaatcgc
     2701 tgcggattta gttgttgcat ggctaaatca cgaatcacaa gtatctacta aaggcgaatc
     2761 aatttcgcca aaagaaattg ttcaagctta tttagatatt cattatgctg ttatctatgg
     2821 tcagcttcca gaagaccgta aaaacgatga ctaaacgtga gctaaaatct ctgctatggc
     2881 tgcaaccatg gcagagtctt ctttatctac cgcgtgttcc atcgccactt ttgcttgttt
     2941 cagtatgtct aatttcaaat cttcgatttt tgccgagact gttttttcca tattcttctc
     3001 tcctttcttt tatattcgta aacaaattta acaact
//
LOCUS       DAZULY010000252         3019 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000252 DAZULY010000000
VERSION     DAZULY010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.256"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..744
                     /gene="fabI"
                     /locus_tag="ACG81A_002561"
     CDS             <1..744
                     /gene="fabI"
                     /locus_tag="ACG81A_002561"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFS5015876.1"
                     /translation="EGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERMKKSL
                     VKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNVSDIS
                     RDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAKASLE
                     AAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVTIEEV
                     GNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(854..1906)
                     /locus_tag="ACG81A_002562"
     CDS             complement(854..1906)
                     /locus_tag="ACG81A_002562"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFS5015877.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            2032..2952
                     /locus_tag="ACG81A_002563"
     CDS             2032..2952
                     /locus_tag="ACG81A_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFS5015878.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT          875 a          613 c          582 g          949 t
ORIGIN      
        1 gaaggaaaaa aaatcgtcat tatgggcgtc gctaacaaaa aaagcattgc ttggggttgt
       61 gctgaagcaa tgttgaaaca aggggcagaa attatctata cttaccaaaa tgaacgaatg
      121 aaaaaatcat tagtcaaatt ggtcggcgaa gaagcattca ctgtagaatg tgatgtttct
      181 agcgacgaaa gcatcgaaaa agcaatgaat gccatcggtg aatatgctgg ggagatccac
      241 ggccttgtcc atgcagttgc ttacgccaac aaagaagaat tgtccggaaa tgtgtctgat
      301 atttccagag acggctatca attagctcaa gatatcagca gctactcttt aattgctgta
      361 tcaaaatacg cacaaccgtt tcttgcacca aacagcggga tcgttagcat gacttatcta
      421 ggttcagaac gcgcaatccc gaattataac atgatgggta ttgcgaaagc ttctcttgaa
      481 gctgctatcc gttatttggc agctgagttc tcgcctaaag gaattcgggt caatggaatc
      541 tcagctgggg caatcaaaac attagctgtc acaggtgtta aagactatca aaaactgatc
      601 cagttatctg aatctcgtac gcctgatcag caaggtgtga cgatcgaaga agtcggtaat
      661 gcttgtgctt tcttaatcag cccactttcc agcggaatca tcggtgatat tatctacgtt
      721 gacaaaggtg tccatctttc ataaactttt gttgcattct ttaaagtaaa acatataaaa
      781 atcaggaaaa aactttttaa agtctcttcc tgattttttc tttttacgtt tttttcaact
      841 cattactttg aaattattcc acgacttcaa tccagccttc tggtgcttct atgtctccaa
      901 attgaatacc tgtcaattca tcatacaacc gttttgttac tggtcctact tccgtttcac
      961 tataaaatac atgaagattg ttattgtgat aaatcccacc gattggagaa ataacagccg
     1021 ctgtaccaca tgcaccagct tctgcaaatt gatctagatc atcaatgaaa atatctcctt
     1081 caattgcttg cagtcctagt cgttcttcag ctaactgcaa caaggaatat ttcgtgatac
     1141 ttggtaagat agagggcgat tgcggagtga tgaattttcc gtcttttgta attccaaaga
     1201 aatttgctgc acctacttct tcgattttcg tatgtgtggc tggatcaaga taaacacaat
     1261 ctgagtaatc cagttctttt gcttctgttc ctggaagtaa actcgctgcg taatttccgc
     1321 cgacttttgc tgcaccagtc cccttaggag cagcacgatc gtaatcagat acaataaaat
     1381 tcgttggttt tagtccacct ttgaaatacg ctcctactgg cgtacagaag acgacaaata
     1441 aatattctgg agctggatgg acaccaatgt tttcaccgac cccaatcagt aaagggcgca
     1501 agtataatgt agcgcctgtt ccatagggcg gcacaaaatc ttcatttgct tttaccactt
     1561 gcttgacagc atcaacgaac atcttttcag ggaccattgg catcagcaat cgtctagcac
     1621 tttgattcat ccgtcgacta ttttgttcag gtcgaaagag attgaccttg ccgtcttttc
     1681 gacgataagc ttttagacct tcaaaacatt gctgaccata atgaagcgct ggagagcctt
     1741 catgaatcgt caggtaatta tcttctgtca attctccttc ttcccattgt ccgtctttcc
     1801 atttggcaat aaaacgccaa ggagttttga tataagaaaa acctagattg ttccaatcga
     1861 tttgcatata cttaccacct tcgttatttt ctccacattt tatcattatc catattattt
     1921 gtcattttat tttcagcttt tttccttcat tatagttatt tttgctattt ccttttttga
     1981 aaacttcttg gtacaataca gaaagaatca aattttagaa aagaggattt tatgttttca
     2041 ttattagcaa ctgccggtac gacagactct tctggcgaga cattgacaga tcaagctgtc
     2101 agcaaagtaa atgcgtttca gcggttttgg gacgggattg attgggacgc cattttggct
     2161 gcctttatcc aaaaaagttt atatctgatt tttttgatcg tccttttttg gattcttcac
     2221 cgtgtgggaa aatatctgat tgataagtct tttaatcagt attctaaaaa agccattcta
     2281 aacgagagcc gcttaaagac tttacatagt cttttaaata ctatttttca ttataccctt
     2341 ggtttctttt ttatctatgc aattttatct gctatcggtg tgccgatcgg atcacttttg
     2401 gctggtgccg gaattgcagg tgtggcaatc ggtcttggtg cccaagggtt catgagtgat
     2461 gttattaccg gattttttat tatcatggaa caacagatgg atgttgggga ctacatcaag
     2521 ctagcaaact taacgattga aggaaccgtc gtatctgttg gtatccgtac ccttcagctg
     2581 aaagcaacgg atggtactgt ccatttcatc ccgaatcgaa acatcaccac gatcagcaat
     2641 ctctctcgtg caaatatgca agtactttta gatattcgga ttgttcctga agaaggatat
     2701 gacaaaatct atgagatcat tgatcgagta aatcgtacat tagctgaaaa ataccaagaa
     2761 gagctccaaa ctgaaccttc tatctttgga ttagtggatc ttggaaacag caattttgcg
     2821 attcgtacgg tctgctacgc attgaacgga aaacaatatg ccttaaaaga agagttcttg
     2881 agctcctatg tcaaagaatt gacagcttct ggtttcacga ttccgaacag tccaattgct
     2941 gtcggaaaat aatttggaca aacgatgcaa cgaatctcac taaaaagagc gtgggacaaa
     3001 agtaaagaac acttttgtc
//
LOCUS       DAZULY010000253         2948 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000253 DAZULY010000000
VERSION     DAZULY010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.258"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..531
                     /locus_tag="ACG81A_002564"
     CDS             22..531
                     /locus_tag="ACG81A_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS5015879.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            624..1786
                     /locus_tag="ACG81A_002565"
     CDS             join(624..900,900..1786)
                     /locus_tag="ACG81A_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5015880.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2066..>2948
                     /locus_tag="ACG81A_002566"
     CDS             2066..>2948
                     /locus_tag="ACG81A_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5015881.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQIS"
BASE COUNT         1051 a          486 c          600 g          811 t
ORIGIN      
        1 attgtttagg gctatactat attggtacaa ggaatagaaa gaggtgttta tgtggttgat
       61 aaaattcagt ttaatcaagt tatagaagaa ttcatcaaag taaataattt aatgacccat
      121 attcaaaaac gaccaatcag aataactaat gatctaaaac tcagtacgag tatgattcat
      181 ttgattgatg tgattggaaa ttatcctgaa actagtatta cggaattagc tgatcgatta
      241 ggcgttacca aaggagctgt atcccaacag attcccactt tagagaaaat gaatcttata
      301 agcatttctc aaaaaaaagg aaataagaaa actaaacttg tatcgttaac aaaatatggt
      361 cgaaaggtgt acgaatcaca taattattta catgaagagc tttatagctc aataaatgaa
      421 gcgttagaat cattctcacc agaccaaatt tcaaccatta aacagattct agaaaaagta
      481 tcttatacaa tcaatgaata tcaagtaagt ctgacaagaa aggaagattg acatgaaaaa
      541 aatgattttt gaatcggcta cactagacta gacagaaaaa ataaggtgtg taaaataaat
      601 gaaaacacac tggaggaatt tttatgtcaa gacgtcaacg tagaacctat tcaaaagaat
      661 tcaaacaaca agtcgtcgat ctttatctcg caggaaagcc ccgagctgaa atcattcgag
      721 attatgagct gactacctcg gcctttgata agtgggtcaa gcaagcgcaa actactggct
      781 cgtttcagga aaaagataac ttgatccctg aacaagtaga actaattgca ttacgaaaga
      841 aaaataaaca gcttgaaatg gagaatgaca ttttaaagca agcggcgctg atattgggac
      901 gaaaagacaa gtaattgata ccaataagca caaatactca atatcagcga tgtgtaaagt
      961 gttaaacatc tcttgccaaa cctattatta tcaagccaaa ccagttgaag atgaatcaga
     1021 actggaagaa ctcgttcaag aagaatttat tcgcaatcga aaagcttatg gtaccaggaa
     1081 gctgaagcag tgcttggcca atctaggtct tcaagtgagt caacgcagaa tcggccgtat
     1141 gatgaaacag cgtggcttgc gttcaacata cacattggct catttcaaat tccattcttc
     1201 tacttgtaac gaagccaaga ccgccaatat tctagacgga accttcctac aggaaaagtc
     1261 attagaagcg attgtaaccg accttaccta tgttcgtgtt gggaagaaat ggcagtatat
     1321 ctgcctgatt cttgatttgt ttaatcgaga aatcatcggc tattcatgcg gggagaagaa
     1381 agatgcgacg ttagtcaagg aagcttttgc aaagattccg tactcgttga ataatgtaca
     1441 actttttcac acggatcgtg gaaaagaatt cgataaccag acgattgacg aaattttatg
     1501 ggcctttaat atcacgcgtt ccttaagcaa aaaaggctgc ccctatgata acgccgttgc
     1561 agaatccacc tataaatctg tcaaagtaga attcgttcat caataccgct ttgagacatt
     1621 ggctaatttg cgattagagc tatttgatta cgtccattgg tggaatacca tccggctgca
     1681 cggcacgtta ggctatgaga caccgatcag ttttcgacag cagagattgg cgcagcgaat
     1741 ccttgataat gagctcggat gtgataccgg tggcgaggca ccttaattat ttgaatgagc
     1801 ttctgcctta gagaatcaga ttcgaggagg ctcgttgtca agggcagtcg gaacaccagc
     1861 agcaagctta cgcaatacct tataattttt gtcaaaaaaa gtgttgccat tccacatata
     1921 tctttaaaca gctattgcca gataaaaata aatgatatta tatatataaa tacttatccg
     1981 aaaaggggaa tgagagagtc aaggttcttg gaatgtcgaa tagtcgagta ggtagatttt
     2041 ctatctacaa ccttgttgtt taaaaatgaa aaaaattgga attagagaag ttgcgaaaga
     2101 ggcaaatgtt tctcctacga ctgtgtcgag ggtattgaat gatcgtggat atttgagtga
     2161 ggaaacaaaa agaaaagttt ttgatgccat gaaaaaatta gaatactatc ctaatgaact
     2221 ggcgagagca ctttttaaaa acgaaacata taccgtaggt ttgatttttc ctacaattac
     2281 taatccattt cacgctgagt taattcaaga tatcgagttc gagctttcca gcaggggata
     2341 taaagtgttg ctgtgcaata gcttaaacaa tccaacaaaa gaaaaagagt acttgacgat
     2401 gttgagaaag aatcaggttg atggaattat agtaggaacg cacaataaag atattgcgga
     2461 ttatgatatt ccagggctac ctattgtagc gattgataga agtcttggtg aaaatacagt
     2521 tacagtatct tgtgataatt acaggggtgg ggaattagct actcaactac ttgtaaatca
     2581 tggctgtcag aatatactgt gtattcgggg agatagtaag ataaagctac ccgcgaatag
     2641 taggacttta gcatataaag atgttttgaa taaattcatg ctggaaccgt tagtcctaga
     2701 agtaccattt gtaaaagaaa tcaatgaaaa aaatgaaata attaagacat atctggaaac
     2761 tcatccaaat attgacgggg tttttgcagg agatgattta ttggcttctt tggcgattaa
     2821 caacttagga agttcgggca aaagagttcc aaaagaggtt aaagtgattg gatttgatgg
     2881 agctcaacaa acattgattt ataatccaga attatcaact attcaacagc ctatagatca
     2941 aatatcta
//
LOCUS       DAZULY010000254         2946 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000254 DAZULY010000000
VERSION     DAZULY010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.259"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..717
                     /locus_tag="ACG81A_002567"
     CDS             <1..717
                     /locus_tag="ACG81A_002567"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFS5015882.1"
                     /translation="INPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIA
                     SPFMLFTAILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSND
                     ARVTPVGKFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNV
                     RAGITGYAQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLE
                     EDENVISLAEIAFPNEKIFR"
     gene            733..1773
                     /locus_tag="ACG81A_002568"
     CDS             733..1773
                     /locus_tag="ACG81A_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5015883.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            1792..2877
                     /locus_tag="ACG81A_002569"
     CDS             1792..2877
                     /locus_tag="ACG81A_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFS5015884.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
BASE COUNT         1073 a          420 c          557 g          896 t
ORIGIN      
        1 atcaatccaa atattatgaa tttcgaagat gaaagtatta ttgaagcatc tggatttaga
       61 atccaacctg aatatgaact attcaaacgt ttgtttgata tcatcgtttc acttgtttta
      121 ttagttattg cttcaccatt tatgttattc acagcaattt tagtcaaagt gacatcaccg
      181 ggaccaatca tctataaaca agtgcggatc acaaaaaatc aaaaggaatt ttcaatctat
      241 aaattccgct cgatgaccgc cacagccgaa gcaaaatctg gaccagtttt agctaagagc
      301 aatgatgcac gagtaacgcc tgtaggtaaa tttattcgag ctgtccgctt tgatgaatta
      361 ccacaaatct tcaatgtgct gaaaggcgat atgtcgatcg taggaccacg tcctgaacgt
      421 tctttcttcg tcgatcagtt caatgcagaa aacccttatt actacttacg gcataatgtt
      481 cgtgctggta tcactgggta tgcacaagtc tatggaaaat atgtctctga ttacaacagt
      541 aaattacgtt ttgatttact ttatatcaaa aaatattcat tgatgcttga tttaaagatc
      601 ttgctgcaaa ccatcaagat cctatttgat aaaatgtcct ctcaaggact tgaagaagac
      661 gaaaatgtga tctcattggc agaaattgct tttccgaatg aaaaaatctt tcgctagttg
      721 ggaggcgtaa aaatgaacaa tccgaaattc agcataatcg taccagtata caatgcagaa
      781 gcaactattg gtcgaacgat tgatattcta aaagaattgg attatgaaga gtacgaagtc
      841 gttttagtaa atgatggatc aacagatcaa acacaggaaa tcattcaaga actgattgaa
      901 aatgatgaac gttttgaatt gattactacg accaatcaag ggcccggatt agcaagaaac
      961 gaaggaataa gacagtcaaa aggagaatat ctcttattct tcgatgcaga tgacacgcca
     1021 gtaaaaacga tcttgtcaga ttatgatcat ttgctagaaa agagtccaaa agcggatctt
     1081 gttgttagtt cgtttgtttt tcgtacaatg gacgagggaa gaatcgtttc cgaaaaagaa
     1141 aacttagtta cagaatgtca ctatctaact cacgaagagt ttataaaaga catgtatcag
     1201 ttaatgaatg aacagttgat gtatgttgta tggaacaaat gctatcgtag cgatattctg
     1261 aaaaataacc aaattctatt taaaacttat aatagttgtg aggatcgaat atttaactta
     1321 cattattatg aatactgtca tcaagtgatc atgaacccaa aagtagaata tatttatgaa
     1381 tttgaaggtg ggaaggggat cactaatcaa taccgaccta acaagttcga aacatttaaa
     1441 gaattctatt cattagcaaa tactgtaaca gacgatatta ataaagacgg tatggcggcg
     1501 cttcttttaa aagggacaac ttcagttatt ttttctattt atagtacctc tgcaagaact
     1561 tcaaaagaga agaaagacga agcaaagcag atattgagtg atccagtgat ccgagaagcc
     1621 aagcaaattg ctaagacgga ttcttcagca aaaaaaataa caaagttgct ttacaatatg
     1681 ccagctccac tatttttatc tgctgtaaaa gttggaagct tcgttgaagt gaaaatgcca
     1741 gggttaatgg ctgctttgaa gcgagtatat tagttaatgg aggaaaatat aatgaaaaga
     1801 attttgcatt ttcagggacg aatgggttta ggtggagcag agtctttcat gatgaattta
     1861 tatcgtaaaa ttgatcgtac aaactatcaa tttgattttt tgatttatga agattatgca
     1921 gatgttcaag actatcattc agagattgaa agattaggag gccgtatttt tgttgtacct
     1981 aatcctaaga aaaatatttt taaatattta atagaagtga ataagttact aaaaaaagaa
     2041 tctttttcta tagttcacaa tcaggtttat tttggcgggg gaattaattt atggttagcc
     2101 aaaaagaatg gaatcagaca gagaattgct catagccatg caactgaaga tggaaaatct
     2161 caaaatattg taatgaatgt attgcgtaaa tttttaacca aattgttgtt acaaaatgca
     2221 acagactacc ttgccgttag tcaagaagct ggtgaaagtc tgtttcaaaa ccatcccttt
     2281 gagatagttc ataatggtat tgatttagag ctttatagta aaaattcaga agctaaggtt
     2341 aataagagaa aagaattgga tatctcaatg agtacttttg ttgtgggaaa cattggacga
     2401 ttagagaaac aaaaaaatca atcttatttg ttagagattt ttttagaaat actaaaaatg
     2461 aattctgatt cttacttgat tttagtaggt gaagggtcac ttagaaataa actgaaagcc
     2521 aaagcagtag aattggatat agagaaaaga gtaaaatttt taggagaaag aaacgatatt
     2581 cccgagttat tatctgtgat ggatgttttt gtaatgcctt ctctttatga aggtctgcca
     2641 atggttggat tagaagcaca agcagcaaaa ttaaaattag tcttagcaga tactatttcc
     2701 aaagatacaa agttaacaaa taatgtggat tttgtatcat tagatgaaac accacaagaa
     2761 tgggcaaaaa aagtaataaa agagccgtat gctaataaaa taacacaaga actaaagagt
     2821 tatgatgttt cgtacactac tgaacaaatg gagagtatat atagtagaga tcattaaaaa
     2881 tgttcaagat attttaggag tgattatttt tgaaaatatg ttatttgata ttggcataca
     2941 atgata
//
LOCUS       DAZULY010000255         2922 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000255 DAZULY010000000
VERSION     DAZULY010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.260"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..1336
                     /locus_tag="ACG81A_002570"
     CDS             2..1336
                     /locus_tag="ACG81A_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015885.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1323..>2922
                     /locus_tag="ACG81A_002571"
     CDS             1323..>2922
                     /locus_tag="ACG81A_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFS5015886.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYL"
BASE COUNT          993 a          440 c          499 g          990 t
ORIGIN      
        1 gatagatgct gcttttttat ttagttgttt atatactttt gcaacttatc gaacagttga
       61 tatctattat cgatccgcaa ttgcagaagc aatcgctctt acgattatcc cagctgtttt
      121 atattatgct tatggattga tattcaatag agaaaaaaaa gtcatcgctt tatctgtcag
      181 tataagtcta cttgtgtata ctcatgtctt aactacacta ttgtgtagtg cattgattgc
      241 ttgtatgatt atagttcgtt tgtttttaag caagcaaata aaaaacgata taaaatttgt
      301 ggtcgtgaat ttagtaaagg ctgttttttt gacttgtgca ttaactagct atttttggta
      361 tcctatgttg cagcagatga attatcaaga aatacacagt cccttcaaaa cagatttgca
      421 gctagaagca ctttctattt cagatagtat cattcaagcg ttaaataacg atattacaac
      481 atacactatg gggttattag gattggttgc gttgattttt cctttattca tactgaaaga
      541 aatggagaaa aaagaaaaaa tcatctatgt tggcgtagtt tctacatggg tatgcgtaac
      601 taatttattt ccatggtttt tgtttcaaaa aacaccgata agcttactcc agtttccttg
      661 gcgtatctta ggtatccaaa tctttttttc ttcaatgatc ataacgcttg tttttatgaa
      721 aaagactatg aataaaaaat attcatggtt aatcattggc gctactgtgg tgtttctact
      781 cgtcacgtct atagcagcaa aagaaaatta cgcaaaagtt atccaatcaa aacacggtca
      841 tattgttatc aatgaagaaa cactcccacg ttatacaaca tcagatatgg gaggattata
      901 cgattacgcc ccaacagaag cgataaaaga aaggcctcac ttagaaaaac atgaagtaaa
      961 agtaggagaa tcgtgggaaa aagggaagta taaagctgca gataataaca ttacttacac
     1021 agtggcaagc aacaaaaagc aaaaagtgac agtaccagta tatgcttatt taggtacaca
     1081 ggtaaaagta aacggtaaag tcgtaaatga ctcttataaa aaacatggct tagtgaatgt
     1141 ggatgtaaat gctggagaaa ataagataga aattacaaca aaatatactc caactgctct
     1201 catggcttta tggatcagta ttctgactta ttgtttagtg gtgtattcaa gttttaaacg
     1261 atttgcacct aataagttga aagattctcg atatgtgact aaaaaggaga taaacaagaa
     1321 gaatgaaaat aaataaaaaa gtagtaatat tgagtggatt aattatagtt agtagtatat
     1381 attctttcat ctttttcaaa aatacacaga gtatttatac cactggagat ctttcttttc
     1441 acttatctcg aatcaagggg ttatcgacta tttttaccag tccgattaat tatgagacat
     1501 tcaattatac tggatatggt gtaaattatt tttatccctt tctgactttt tttccagctg
     1561 taatgttata ttggatgaca aaaaatttga ttgtaagcta tattatttac gtttggttat
     1621 tgaacttgtg tacaacactc gtagcttatc attatggcga gcggttttta aaacaaaaaa
     1681 aagcggcatt tttatttagt tgtctctata ttttctctgc ttatcggaca gtagacatct
     1741 actatcgttc cgcaattgcc gaagctatcg caataacact tgttatccct gtattatttt
     1801 atgcttatca aatcatctct ggaaaagaag aaaagtatcc gtctgtaaag ctagcattga
     1861 gtatgagttt gctagtgtac agccatgtac tatcaacatt aatgagtaca gcattaatta
     1921 tcatttttat ttttattcgt ttggtttcta aaggtttcaa gaatgcagat tttattgcta
     1981 tatttaaaaa attatttagt gcggcaggaa tgacattggt tctgacgagt gctttttggt
     2041 atccgatgtt tgaacaaatg ctctaccaaa aaatcaacaa acctagcgtg acaaatttat
     2101 atgctcatgc atctaatgtg ttcgatagtc tgactgaagc tatgaacaac gacttaacta
     2161 cttactcaat gggactagtt ggtctgttat ctttatgtat tcccctgatt ttatttaaaa
     2221 aattaacaaa catagaaaaa aagatttatt atggtacgtg tttgacttgg ctagctacaa
     2281 ctagtttggt gccatggtat cttctgcaaa atacgccagc caaattgtta caatttcctt
     2341 ggcggatctt aagcttgcaa ataatattct cttctctgat attaacgatg atatttttca
     2401 aaaatagaag atacaataag acaagagaac tattttattt atttatgact gttttgttat
     2461 taatcacttt aactgtttct agtaaaaaaa atttcaatca aaaaataatt tctcagccgg
     2521 gacatattgt tgtaaacaaa gaaacagtag aggcgtatac cactaaagga atggattatt
     2581 ttttatatga ttattctcct atcgcttcat tggagaataa ggagcatata ataaaacacg
     2641 aagcaaagat tggcagtcaa tgggtaaacc ttccttataa aacaactggt agttctatca
     2701 tatatagagt aactaatgag aagagccaag taatcacact tcctgtatat gcttatttag
     2761 gaacgaatgt agaaataaat catcaacctg taaaggagca ggtgaatgct aatggattgg
     2821 tacaaataag cggagtcaaa ggagttgctg atattaaagt aagcacaagt tatacagtaa
     2881 cgactattat ttcttatctt gttacattaa ctgcatattt at
//
LOCUS       DAZULY010000256         2903 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000256 DAZULY010000000
VERSION     DAZULY010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.261"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..497
                     /locus_tag="ACG81A_002572"
     CDS             <1..497
                     /locus_tag="ACG81A_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFS5015887.1"
                     /translation="IKEFIPEGVTFLKRSSSLDQDTTSITQVLAGFTKEVEADVYVMT
                     HTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGEPFNYELDNIPRTQDLP
                     PLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDIDEAEDFMIADAIYNHLI
                     LQGS"
     gene            516..2099
                     /locus_tag="ACG81A_002573"
     CDS             516..2099
                     /locus_tag="ACG81A_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HFS5015888.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            2228..2812
                     /locus_tag="ACG81A_002574"
                     /pseudo
     CDS             2228..2812
                     /locus_tag="ACG81A_002574"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
BASE COUNT         1087 a          323 c          551 g          942 t
ORIGIN      
        1 atattaaaga atttatacca gaaggtgtaa catttttaaa gagatctagt tccttagatc
       61 aggatacaac gagtattaca caggtattag ccggtttcac aaaagaagta gaggctgatg
      121 tatatgtaat gacacacaca acagcaccat ttattagcga aaaatctatt gaaaaaggac
      181 tagatgccgt gctgacagga gaatatgatt ctgcatttgc tgcaaaaaaa ttgcaagatt
      241 tcttatggaa agacggtgaa ccatttaatt atgaattgga caatattcct agaacacagg
      301 atttgccgcc tttatacgaa gaaactagtg gattttatat ttataatcgc aatgtgatga
      361 ccgagttgca tagaagaatt gggaaaaaac cttgcatagt tgaagtaggt gagattgagt
      421 cagttgatat tgatgaagcc gaggatttta tgattgctga tgcgatttat aatcatctta
      481 ttttacaagg tagttaagac agggggaagg ttaggatgaa caatgtacag attttagatt
      541 gcacacttag agatgggggc tattgtaaca attgggaatt tggatttgat aatacaaaaa
      601 aaattatttc tggacttata gaatcaggaa ttgagattat agaatgtggg tttctcacaa
      661 ataaaacagt atacaataaa aatgttacaa aatatacgac cttagaagaa ttagcacaga
      721 taattcctga aaaaagagaa ggaaaattat ttgtagcaat gatgaactat ggcgaatata
      781 atatagagga tcttccagat tatgatggtt catctattga tggaattagg attgcgtttc
      841 ataaaaaaaa ttgcaatgaa gcgttgaaat tatgtcgtga gataaagaaa aaaggctact
      901 tagtttttgt tcaagctatg gtatctctca attatacaga tgaagagttt ttagcagtaa
      961 taaaaaatgt gaatcagttt aaaccatacg ctttttatat tgttgatagt tttggaatga
     1021 tgaaaggaaa agatttaact cgtctttttt acatgattga gcataatcta gaaaaagata
     1081 tacagatagg ttttcactca cataataaca tgcagcttgc atattctaat gcacaaaaac
     1141 ttataaccgt acaaacaaat cgaagcctga ttattgattc ttcggtgtac ggaatgggaa
     1201 gaggagcagg taatcttaac acagaactat ttgtagaata tttgaatgat aatgcaggaa
     1261 aaaaatatca attaaaacca ttattatata taattgatga aatactaaat agtttctatc
     1321 agaagagtta ttggggatat tcattaccaa actatatttc agcagttcat aatgctcatc
     1381 ctaattatgc aagctatctg tcatataaga atactttaac aattgaagca atgaatgaaa
     1441 tattcgaaat gatggatgaa gaaaaaaagg tatcttatga taaacaatat atagaagatt
     1501 tatactacaa atatatggaa actgggaaaa tacaggagga atatatggaa gaactgagaa
     1561 aatttctctc agacaaaaaa attctactga ttgcaccagg aaaaagttct gtagatgagg
     1621 tagaaagaat aaaagagttt gttaccaaag aagaagtcgt aataattagt gtaaattttg
     1681 aatattctca tatagatata gattacgtat ttttaagtaa tttaagacgt tttcatgaat
     1741 taccgaaaga aagcagaact aaatgtattg ttacatcaaa tatacaagca tcggatgtat
     1801 atttacggac gagttataaa aagttgttat ctgaaaggga agttgtaaga gacaatgcag
     1861 gattaatggc aattagattt ttagccgata tgaatgtgga tgaaatattc ttagctggat
     1921 ttgatggata ttcacataat gaagatgaga attatggtga gcaatccatg gaaattataa
     1981 caaaatttgc attattagat gcaatgaacg tgcaaatgag agatgaattg tcagaattag
     2041 ctaaaaaagt aaaggtgaca tttttaacgg atccacgttg ggttcgaatt gataagtagt
     2101 agtaataatg tttttaaaat tttgataaag agatagtgag agaataaaaa tgggtaaagt
     2161 cttaaataaa aaagaagaaa aatatttaag ggacgatggg gcgagatgaa actctaattt
     2221 tgaactttta agaatcattt ctatattgtg tattgttgct taccatgcta atttgcgttt
     2281 aaattataat gcagacggaa ttatagtaca tggttcatta actgctaacc agattttctt
     2341 gaattatatt ggtatttggg gtcagctagg agttaattgt tttatattta tttcggcata
     2401 ttttttaagt gagaaaagaa acattcactt tgaaaatgtt ttgggaattg tattagaatg
     2461 tattttttat agtgtattag tttatggaat tctttgtgca tgtggaatag aaacattttc
     2521 attgaacagc tttgtaaaag aaatattatc atttgctcta ggtagatatt ggtttattac
     2581 atcctattta gcattgtata ttctaagtcc gtatttaaat atcatgctta acaatctgga
     2641 aaaacttcag ttgcaaaaaa taagtatgtt attgtttata ggtgtatgct gttatcgtat
     2701 gttatttaaa acatctttgt ttggggattg tgctgtttta attgcactat atgtcatatg
     2761 ttatacactg aaaaaaacga taataatatt tttgagagac acagaatcat aggaacaatt
     2821 gtatgtataa cagttatacc acttctcata tccataggaa tacttttcaa atttatacca
     2881 aatgcaatgg ttaaccagat ggt
//
LOCUS       DAZULY010000257         2860 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000257 DAZULY010000000
VERSION     DAZULY010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.262"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..801
                     /locus_tag="ACG81A_002575"
     CDS             34..801
                     /locus_tag="ACG81A_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5015889.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            791..2833
                     /locus_tag="ACG81A_002576"
     CDS             791..2833
                     /locus_tag="ACG81A_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS5015890.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT          910 a          469 c          583 g          898 t
ORIGIN      
        1 atagaaatga aaaatgaaag aggaggagaa gaattggaaa agatgatcga agtgaaacat
       61 ctaagtaaaa gctacggcag caaactttct cccgtcaaag ccttagataa tatctcattc
      121 acagtagaag aaggcgagtt tgttgggatt atgggcccaa gtggtgcagg aaagacaacg
      181 ctgatgaata tcttgtccac gatcaatttg cctacgatgg gcagtgtctc gatccaagga
      241 aaagaaatca caaaaatgaa aaacagtgag ctcagtgact ttcgtcgaaa aaaactagga
      301 ttcatttttc aagagttcaa tttgatcgat accctaaatg caaaagacaa tatcctattg
      361 cccctagcag ttgaacggat gactaaagag gaaatggaaa agagagtaag acatgtagct
      421 caattgttga atatagaaga actgctcaaa cggtatcccg atgaactttc tgtcggacag
      481 agacaacgaa ttgcagctgc tagagcactt gttgttcagc cacaagtgat ttttgcagat
      541 gaaccaactg gctctttgga ctcgaagtcg gctacagaat tgttgaacta cttgaagacc
      601 atgaatcaaa aagaaaaagc aacgatactg ttagtcaccc atgatccata tacagcaagc
      661 tactgcgata gaattttatt tattaaagat ggtgtgattt tttctgaagt tgtcagaaga
      721 gggacaagaa gagagttctt tgagaaagta atagacatgc aggcgacgat tggcggagga
      781 ggaaaaatga atgctgtata aactagccgt taaaagtttt tttcggcagt cacgaggcta
      841 tcttgtttat tttttcagtt tgactttatc cgtaatgatc tattattcct tcagcgcaat
      901 gacctacgat caatctttag tcagaagagc aagtcaagat acttcgatag atggtggatt
      961 gagttttggt ggatttatca ttactgttgt tttgcttttt ttcgtattca gtgccaaccg
     1021 atttttcttg aatagaaggc aaaaggaaat aggcatctat cagctttttg gtatgaataa
     1081 actgcagata tcaggtatat atgtgttaga aatcatgata attggattgt tcgcctgtat
     1141 ttctgggata ttactcggta ttattttttc caaactcttt tcaatgattt tggttcgaat
     1201 gatggatatg gatcttacta gtccattttt tatctccatt ccatcagtag tagatacttt
     1261 atttgctttt ttcatcatcc ttttagcagt atctgtatat tcaatatgga aaatatggcg
     1321 ctatccaatg attcgaagtt ttggagaaaa agaacaagca gatagttcaa caatgcgctt
     1381 caggacaagg caccgattac tcgcagtcat cgggttacta cttttaacga gcggatattt
     1441 tggagctagt cattttaggg aaatcacttc atggatgatc agttatcatt atcttgatac
     1501 attagttttc ttttttcctt ttttaatttt tttcgtttgt gtggtcggta cttatttgtt
     1561 tttctgttat acatttcggt tatttttgat catattgagt aaatggaaga tttattacta
     1621 tggtatcaat tttttgataa tgggtaacac gcaagttcat ttgctcaaaa gttggcgtac
     1681 aaattctctg attacagtca ttctcggaat ttctttaatg acaattggcg gcacggctag
     1741 tatctctact attttatcct accaggagaa agtttcctcc ccgatggact atcagtttga
     1801 tgttgagaca gagaaaaaaa tcaagccgat tctagcagag gaagggcaga agataactaa
     1861 agaaatcacc ttgcattata aagtcattgg gagtatctat agtcaaaatc tcgaagaacc
     1921 tatgtttcaa actgctaatc tgatcacgga aacacaatat caagccttca gaaaagttaa
     1981 tccagatttg ccgaacattt ctttgaaatc acctggtcat actgttttgc ttgatcaaat
     2041 gcaaactctt tttcggaata tttcattata tggatctagt attggactgc cagataatca
     2101 agaattgaag attcaacaaa tgcttccaag tgtattaggt gacgaaacaa tggtatatct
     2161 aagtcctgta ctgattgtag atgataccgt ttttgaaaaa gcaaaaggtg tggattatca
     2221 agtagtcaat gtagatgtat caggtggaaa taatgaaaaa atagatgaac gactgagcaa
     2281 tgacctgtcg ataaagtggc tcaatgctgt ttattattct tatgatatcg taaatggaag
     2341 tctacaagga gaaattagca caaagaaact tccggatgac caaatgaagg aagcacaatt
     2401 tagccaagaa agctctcgtt tgaatttctc ttccagctat tcgaaattgc gtgcagttaa
     2461 tcggcgaatc ggtatcaata tttttgtcac actatttgtc gggatgatcg tgattattgc
     2521 tactggtagt attttgacag tccggcaatt agcagaagca gaggaagaaa aagaaaatta
     2581 tcacctattg actaagctgg ggattccaca aagaaggatc agacggatga tcttcgagca
     2641 aaacgcactt accttctttc cacccatgat attagggatc actcacgcag tatttgcaat
     2701 caatgtcttc actcagtata taaaaactgc cgattactgg ttggcttatc ttgtgagtgg
     2761 cttattgatc cttatctatc ttttgcttta tgtcattacg agtcagttat attgtcggat
     2821 tatcgaggaa tgatatacgg aataagacaa gataataaat
//
LOCUS       DAZULY010000258         2844 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000258 DAZULY010000000
VERSION     DAZULY010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.263"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..494
                     /locus_tag="ACG81A_002577"
     CDS             144..494
                     /locus_tag="ACG81A_002577"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5015891.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            908..1726
                     /locus_tag="ACG81A_002578"
     CDS             908..1726
                     /locus_tag="ACG81A_002578"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFS5015892.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1799..2158)
                     /locus_tag="ACG81A_002579"
     CDS             complement(1799..2158)
                     /locus_tag="ACG81A_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS5015893.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          969 a          399 c          505 g          971 t
ORIGIN      
        1 gcaaccggag tttcttgatg aaaatatgct tctatcatta caagctcgtt tggtgtaaga
       61 tgggtataag tcatttatgt tcactctcct tgtatgcttt agcgggtatt acaatttgag
      121 tgtaacataa atggcttttt tatttgtctc gcttaattat acaaacggcg ttttataaag
      181 caatcattcc tgagaactta gatgaggtta aactttattt atgggaagat aacggagaaa
      241 ttgtcggttt tagtggtgta agtgcagaag aattagttat gctatttctt gatccagatt
      301 ttattggtaa gcgatatggt agtcaaatat taattgagct gattgaaact gaagatataa
      361 aaagaattga tgttaatagt caaaataaat atgcaaaaca tttttattta agccatggat
      421 ttgaaattga atctgaagaa gacttagatg gttttggtaa accataccct ataacgcatt
      481 tagtaaaaaa ataattcatg aatggtttat tacctgattg aacatgaata aaagtaatat
      541 ttttattttg atggcctatt tctaggttat gaattatcca gatcaaattt gtgtattaca
      601 ttcagtaata aggtgcttat cttaggatca acttatgata ctagctgacc aaggatgtac
      661 cagttataga aaatcagtta aagtaaaaat gataaaaatc ttatgtcaac actgatttag
      721 gctgacctgt gggttaggag aaagacaggt cggtagcttt taatacaaaa aatggcttgt
      781 atgtatctag atagttaact gaaattagaa atgttcttat ttttaacgga gtaagaaaaa
      841 taggctgctt taggtctgaa tactttttca atataatgaa gatatcaaag gcgacaggag
      901 tgtttttatg aataagaatc ctcataatct aaagttggta gcagtagata tggatggaac
      961 atttgctcgt actgattaca catacgatat cccaaggttc aaggctgttt tatcgcgaat
     1021 gaaagctgtt gattgtcaat ttgttgttgc aagtggaaat caatactacc aattgagatc
     1081 gttatttcca aattattatg atgaattaag ttttgttgct gaaaatggtg cttttgttaa
     1141 agataaaaca gaggtactat tttcagccga tatgcctaga aaagcgatcg aggtggtcct
     1201 tgatttatgt aaagactatc ctgaagtaaa aaatgtgatg tgtggtctga atagtgccta
     1261 ctgtgaaaga ggaaaggtga gtcaagcatt ttttgatcta accaatatct attaccatag
     1321 attagaatgg gtagctgatt ttgcagaagt tcaagataaa attttaaaat ttgctccgac
     1381 tatgcctgtt gaaaaaaccg aattttacgt tgaattgttc cgtgaaaaat tagccgggct
     1441 attagtacct accagtagtg gtcatggttc tgtagacttg attctttctg agtgccataa
     1501 aactttcggc ttgaaaatgt tagttgagcg cttggggatt aatcctgacc agtgcgttgc
     1561 ttttggagat gggggcaatg acattgagat gttggagtat tgtggtctga gttatgaaat
     1621 ggataatgca acggaagctg ttaagcaagt tgctaagcat caatgtcctt caaatgacga
     1681 agacggtgtt ttagtgacgc tcgatcgatt gttccctaac acgtaaacac taggtaattc
     1741 atcaaaaaac ctcgatataa gaaatattcg aggttttaaa actatattta gaacagattt
     1801 acgaagcgtc tgatgagtca aaatagactt ggacattgct atctaaaata ccactttcat
     1861 tctttaaacg aattgtttga tttgatggag ttacctctaa tactaaatgc ccattaataa
     1921 aggtagtatc gtattcagat gttttaactg ttgcttcttc gcaaggtatt gttaaatcat
     1981 atgctggaat ataatgttgt gtggaaacta ataaaaaatg atcaccaatc atacagcatc
     2041 cctctgctga agaaaattga ttagagccat catttaaagt taacaagaat ccatccttta
     2101 aacgaggtac ttgcttaaat acttctggtt gtacttctaa aaataaatta tcgctcattt
     2161 tcacgaccac catcgacttc cacagttgaa attgacattt tatttaacac acctattccc
     2221 ccttaactat aactcaattc tatataaaaa atattctatt ctcaaataat tgaacttttc
     2281 aaatcgccaa tttacttcgg ctttatcaga gctcattcat atcaagagcg attaacgctg
     2341 taaaaattaa accgaagatc tgataaattt tctctatttt ctacaatatt taaaacgctt
     2401 ccttagtcta ttatatatct atttctctta ggagtatgtt tagatgtttg aataatagtt
     2461 gcccaacttt aaaggttatt cttataatat tttacttcat tttccaatag aaaattcaaa
     2521 ggattactgg tttttctaaa atagtttagt ttaaatacat aagtatcctc cgacactgta
     2581 ttagttcata atcatctaaa tacaaaattt tcaaataaaa atatttcaag ctctatcgtt
     2641 gacgaatgta aacgatactt ataaagtaat agaaattgat tacatttgta aacttaaaat
     2701 ggttaattta tctcacataa aactaagaat aaaataatga ttaaaagtgg ttagttttta
     2761 tacgatttag cctatcggta aaaatgaatt agagagtgta aaatattttg tgtaaatgaa
     2821 aaaatccata caaaaaagga agtc
//
LOCUS       DAZULY010000259         2843 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000259 DAZULY010000000
VERSION     DAZULY010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.264"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            367..555
                     /locus_tag="ACG81A_002580"
     CDS             367..555
                     /locus_tag="ACG81A_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015894.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="ACG81A_002581"
     CDS             545..805
                     /locus_tag="ACG81A_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6275 family protein"
                     /protein_id="HFS5015895.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="ACG81A_002582"
     CDS             846..1073
                     /locus_tag="ACG81A_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015896.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="ACG81A_002583"
     CDS             1138..1710
                     /locus_tag="ACG81A_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015897.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="ACG81A_002584"
     CDS             1711..2022
                     /locus_tag="ACG81A_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS5015898.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="ACG81A_002585"
     CDS             2013..2273
                     /locus_tag="ACG81A_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015899.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="ACG81A_002586"
     CDS             2434..2793
                     /locus_tag="ACG81A_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015900.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
BASE COUNT          996 a          432 c          619 g          796 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaa
//
LOCUS       DAZULY010000260         2832 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000260 DAZULY010000000
VERSION     DAZULY010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.265"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            275..2170
                     /gene="asnB"
                     /locus_tag="ACG81A_002587"
     CDS             275..2170
                     /gene="asnB"
                     /locus_tag="ACG81A_002587"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFS5015901.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(2365..2811)
                     /locus_tag="ACG81A_002588"
     CDS             complement(2365..2811)
                     /locus_tag="ACG81A_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFS5015902.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
BASE COUNT          921 a          485 c          535 g          891 t
ORIGIN      
        1 gacacgatct ttaggtcgtg tctttttttg ctggtttttt tggaaatagc atccactaat
       61 atttttttaa cacgaagaga ataaggtagc ctcaaaaaag aaaacatggt aaattagata
      121 ttgggacttt attttctgta aaaacagaaa ctttcatctt ttgttttcac gtgacgaaat
      181 tgtaagagag aaaatattcg tccgctattt attctatgat aagatgcata tgaatgaaat
      241 aaacagtaat tttatattag gatgtgaaag ttaaatgtgc ggtattgtag gttttgttaa
      301 cgacaaggac aacaaaaaaa caattattaa tacaatgatg gatcgaatcg ttcaccgtgg
      361 acctaatagt tcaggagaat atatcgataa acatgttgct ttaggattca gaagattaag
      421 tattattgac ttagagggcg gtactcagcc tatttacaat gaagatcgga caaagatcat
      481 tatttttaat ggcgaaatct ataattacca gcctttgaga gaagaattga ttgcagctgg
      541 ccacgttttt caaacacatg cagatacaga agtccttttg catggttacg aagaatgggg
      601 aacagaatta ttgcaaaaga tccgcggaat gtttgctttt gctatttggg ataatgaaaa
      661 gaatgagtta ttcggtgcaa gagaccactt tgggatcaaa ccatattatt atgcagaaat
      721 gaacggaaca tttatgtttg gttcagaaat caaaagtttt ctgcctcatc ctgattttaa
      781 caaagaattg aacaaagaag cgttgaaacc ttatatgact ttccaatatt ctccattaaa
      841 cggcgaaaca ttcttcaaag atgtctatcg tttgcctgaa ggccattatt atacgtataa
      901 agatggaaaa atagacattc agcaatattg ggatgcagat tttgaaacaa aagaaacgca
      961 ttcccgacaa gaatggatcg aaaaaatcga cgaaaccgtt caagcttcta ttgaagcaca
     1021 tacggtcagt gatgtagaag ttggttcttt cttatccagc ggggttgatt ccagttatgt
     1081 aacctctgta ttgaaaccag atcattcttt ctcaatcggg tttgatgata aaacctataa
     1141 tgaagcgatt gaagccagaa aactaaccga actattggac ttagataata cagcagcagt
     1201 gatcgacgga gacatgtcgt tcaaagcatt ccctttgatc cagtatcact tagatgaacc
     1261 tgattcaaat ccttcttgcg tacctttgta ctttttagca aatcttgctt cacaaagtgt
     1321 gcgggtagtc caatctggag aaggggcgga tgaactgttt gcgggatacc aaacgtatgg
     1381 attccatacg aattcaaaat tcatccgtgt gattgctcaa ggattgaaaa aattaccaaa
     1441 aggaacacgc tataatttag gacgtaaaat cggaaaaatg aaaaacttcc atggacggat
     1501 ccatttatat gaatcacttg ctcctgcaaa agaatatttt atcggtcatg ctcgagtgtt
     1561 cgaagaatca gaagcatcag aagtgttaac accaaaatat caaacagcac catctgtgga
     1621 tgaaatcatg actgttcatt acgagaaaac agaaggaatc aaagatgaag tcaataagat
     1681 gcaatatgtc gatcttcatc aatggatgcc aaaagatatc ttgttaaagg cagataaact
     1741 ttctatggca agttctttgg aagtccgagt acctttattg gatatcgaag tcatgaaatt
     1801 ggctcaacaa ataccaagta agtttttatt gaatcaaaac aacacaaaag atattttcag
     1861 acaagctgcg aacaaacatc ttccggaaga atggtcgaat cgtgtgaaat taggattccc
     1921 tgtgccaatc aaagcttggt tgaaagaaga acacggctac gaacaagtca aagcattatt
     1981 cgaagcagat tttgcaaaag aattttttga tcaagaaaaa atcatgaagt tgctagatga
     2041 ccatcacgaa ggacgtaaag aagagcaaag aaaaatctgg acaatcttca gtttcttgac
     2101 atggtataaa gtctactttg tagatgaaac gattccgcaa gcagaagcaa tcgactatgt
     2161 gactgtttga tgaaaatcaa atatcaataa aaaagaggtt ttctcctacc gtttaacact
     2221 gagtgataag aaacaatttc aaaaaacagc tcttcccgtt ttatagatat tatgacttat
     2281 ggttcggagt tggcagagga acaccattgt ttcgaacata agggggttgt gacacgattt
     2341 tcgtcacaac ccctttacat atgttcaata atcgatttct ttttcttttg tcccatcttt
     2401 tttataaatg aacaaagttg attctttgtt ttgtgctact tgctttcctt tttcgattgc
     2461 ttcttttttt gtatcaaaat ggttgcttgc cttttttgcc cctttagatt tcacgatcca
     2521 ttgttcttcc tcatattcta cgatcacatc tgaatcaagc agttttccag cacgaggatt
     2581 cgtatcgtgt tcatccgtct tgcttggatt tttttctttt tcgaaagttc gtttttctga
     2641 ttcactagca tcttcatacc atttttttgc ttgatcaatg gcaattggga tcgcacggtt
     2701 atcagggtac ccttcatcta ataatgcatt tgcgatgtcg attgcttttt ttcttgtcaa
     2761 tttatctaag tttttcattg atgcaggaaa gtccttcatg ttccatggca tattagtatc
     2821 cccctttatt aa
//
LOCUS       DAZULY010000261         2827 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000261 DAZULY010000000
VERSION     DAZULY010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.266"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            799..1053
                     /locus_tag="ACG81A_002589"
                     /pseudo
     CDS             799..1053
                     /locus_tag="ACG81A_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1123..1635
                     /locus_tag="ACG81A_002590"
     CDS             1123..1635
                     /locus_tag="ACG81A_002590"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5015903.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1632..2669
                     /locus_tag="ACG81A_002591"
     CDS             1632..2669
                     /locus_tag="ACG81A_002591"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5015904.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1023 a          496 c          564 g          744 t
ORIGIN      
        1 attgatgacc acgaaagtgc atggcgatat ttgatgttga tactttatgc agtaccctat
       61 gtattcttcg ctatcattct gtggctcata gcattgtttt ttagagtatt taatgacctt
      121 taggtagatt tatatataaa aagggctaaa tacgcaaaat acccccttaa aatgcaaaat
      181 aaatgcaaat aagggggtaa ctccttatga aaaaacaact aatagtatta taacacgttt
      241 ttatgccgag aaaacttatt ggttggaatg ggctatgtgt tagctaactt gttagcgagt
      301 tggttggact tgaattggga ttaatcccaa gaaagtacca actcaacaac acataaagcc
      361 ctgtaggttc cgaccaataa ggaaattgga ataaagcaac gaaaggagtt gagtaaaatg
      421 agtgagaaaa cgaagaaaat cattcgagta tcattagctt tttcacaagt agtagtgcaa
      481 tcattgatag tttattatac ttaccagcaa tataaattat ctaaagagaa ttaagttttg
      541 attatgtaat aagttttggt actaaaaaag acttgatcta attagaccaa gtcttttgtt
      601 agtgttatat taataacaaa ataaaaataa gaagtcgctc actccccgac caaagtttgt
      661 gagcaaaata aacagtcgat gtttgtttct taattgtata tcttgatatt aaacgattta
      721 agattattct tcaagatata tattcgcaaa tgtaattgga aaagcgactt cttaaacaaa
      781 attaaggagt cgctttttat ggcaaaaata acgaaaaatg agcaagaaaa tttggtggaa
      841 aattacacaa aaaaaccggc gtactctaat agccggttag acgcacattt cgtgtgcact
      901 tctaatccga aattgagctt tgacgctatg acgatagtcg gaaaccttaa taaaaacagc
      961 gctaaaaagc tatccgattt tatgagttta gatccacaaa ttagactttg ggatatactt
     1021 caaacaaaat ttaaagctaa agcactacaa gaccccctat gttaggctag ttgtcgtata
     1081 ggataataag ttataataaa gactatacgg aggatgatta ccatgacaaa taaaaacaat
     1141 gaacgttact ctaccgaact gaaagcgaca gccatcaaga aaatgatgcc acccgaaaat
     1201 agggggattc gccaattaag tcaaactttg ggcattccag aagcgactct ttattcttgg
     1261 cgtaaaaaag ctagaattca agggaatact acacccggaa cgaaaaaaaa tgtcgaaaaa
     1321 tggtcaagtg aagataaatt tttaattgtg atggaatcat tctcaatgaa ccaagtagaa
     1381 cttgcggaat attgccgaaa gaagggctta tttaaagaac aaattgaggc ttggaagaaa
     1441 acgtgcttat ctgcgaatga acaggaagaa aatcgaacga gagaacttgc aactgaacta
     1501 aaagaagaga aaaaacaagc gcgtcaatta gagaaagaat tacgtcataa ggaaaaagca
     1561 ttagcggaag ccgctgctct cttattacta agaaaaaaag cacacgcgat ttggggggac
     1621 caagaggaag aatgatttca tcttcagatc gcaaacaagc tgttgaactt attcaagaag
     1681 ccaatcgcaa tggggcccgt ctcacctatg cctgtaatga gctgaatatt agcgttcgaa
     1741 cctacgaacg atggacgcgt gaaggtacga tagcgcatga tcaacgccct ttggcgaaac
     1801 gtcctgtacc caaaaataaa ctgacggatc aagaacgcga aaaaataata gaaaccgttt
     1861 caaaaaaaga atttatgaat ttgccaccta gccaaattgt accaaaactt gcggattgct
     1921 cgatttatat tgcatcagag tctagcttct atcgtgtatt gcgagaaaaa aatatgcaac
     1981 atcaccgtgg ccgaagtcaa gtttcacaaa aacgcatacc gccaagtcac cttgcgacga
     2041 agccaaatga agtttggact tgggatatta cttggctaaa aggtccaata aaaggactct
     2101 tttaccggtt atatctcatc attgatttat ttagccgtaa aatggtaggc tgggagattt
     2161 gggaatcaga agaagctcgt catgccgaaa aacttattaa acagacggtg cttaaggaac
     2221 gtatccaagg acagccgttg gttttacact ccgataatgg cagccctatg aaagccgcga
     2281 cttttcaagt cttactcgaa aaattaggaa ttcaaagttc ttattcaaga ccacgagtga
     2341 gtaatgacaa tccttattcg gaagcggctt tccgaacgtt aaaatatcga ccagaatatc
     2401 ccaataacgg ctttaaaaca atagatgatg ctcgaaaatg gacgaaagag tttgtcgatt
     2461 ggtataacga ttctcatttg catagtggga ttcaatttgt gtctcctgca gcgtgtcatg
     2521 aaggacgaca tctcgctatt ttagagaaac gaaagcaggt ttacgaggac gctaaaaagc
     2581 gccatcctga acgctggtca aaaggtgttc gaaattggac tccacatcaa tcggtggcgt
     2641 tgaatccttt aaaagaaaca gaaagttaac aggagcataa tctttcaaga ttatcaaata
     2701 accctcgtca cccaataggg gaaaaagtta ttcttcccct attggataac gaggccaacc
     2761 gaccataggg aggttctgct ttttgatagg tggaaaataa aaatacgaca actatattga
     2821 caaacac
//
LOCUS       DAZULY010000262         2807 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000262 DAZULY010000000
VERSION     DAZULY010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.267"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            66..491
                     /locus_tag="ACG81A_002592"
     CDS             66..491
                     /locus_tag="ACG81A_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015905.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(979..1680)
                     /locus_tag="ACG81A_002593"
     CDS             complement(979..1680)
                     /locus_tag="ACG81A_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFS5015906.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(1696..2136)
                     /locus_tag="ACG81A_002594"
     CDS             complement(1696..2136)
                     /locus_tag="ACG81A_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFS5015907.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(2136..2711)
                     /locus_tag="ACG81A_002595"
     CDS             complement(2136..2711)
                     /locus_tag="ACG81A_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFS5015908.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT          869 a          559 c          452 g          927 t
ORIGIN      
        1 catccgtttg aagttttaca ttccaatggt ataatttctt tatcctatca aatgggggaa
       61 gtattttgaa gaaattaatc gtactattta ttttgtctgc gggacttttg acattgactg
      121 gctgtgattt gtttggcggc tctgaccaat cctcggacga aacaaccgat tcttctgtct
      181 taaaagcgga aaaagcagat accaccaaca gtgtatatac gctaaaagta cgtaaagata
      241 tggatttatc cgcgggacag ttgacattta cagataaaaa aatggaatgg acacgcacgt
      301 atcagacaca aggaactgat tcttctgaat catctgaaga aaaaacagtg ctgacagata
      361 tcaagatcac taccaaagat gacgaatata ttatttcagg aaaagaaaat aaaaaagaaa
      421 caacagtcat atttacaaaa atcggtaatt accgtttgaa ggatgaggat ggcaacatct
      481 acagtctata gtgccgtctg ttggatcttc ttttgtttct ccccttcttt tctgctatag
      541 taaagaagca agatgattgt aaaggagacg aaatggatga tcgatagtcc tgattattgt
      601 acatgtaata acaacaaaaa tatccataca gaatttaatg accaaggaag cttgagcata
      661 tgcgacaact gtcacaaact agtcaaagat tcatttaaaa catttgaatc ttatcctgtt
      721 caaaattatg taaaaagtag aagaagataa acaaatccaa gcaaaactca ctatggctga
      781 tttttatgaa cttcaaactc agcttcttcc tttgatcaag cttcatcagt aaaacaaaca
      841 gtggatcatt tcagtttaca aatcaaagag taaagattta tttatttcaa atatttgaat
      901 ataaaataac caaacgatag aaaggcttat tctacatcac gtagtaattt ttctttcccg
      961 tcgtttggtt ttttattttt agcctaattc ttgacctttt gcatgctggt tatccgtaaa
     1021 atattttcca gcaggctttc cttccaattc agctaatttt tcttggatct gacggatcac
     1081 cttttttgca tcttctggat tttctacaaa atcgaattta tccccatcca cttgtatttt
     1141 aggacataca tcaaaatctt cataccattg attatagcgg gtgttcaacg tttcataata
     1201 ggtatataac gtctcatcgc tttctagctg ttcatagtca cgcccacgtt ttttgatacg
     1261 ttctagcatc gtgtccaaag aaactttgat atggaccatc aaatcaggcc gtttttttgg
     1321 agcaacatca tctaactcgt ttagcatcgt atctaataag ctatcgtact gctgtacttc
     1381 gatatccgtt acccgaccta aatcagcgtt tagatggaac aacaggctat cttcatagat
     1441 ggatcggtct agcacattgt catcgttcac taaagcattt ttcattgcta aaaatcgctt
     1501 gttcaaaaag aagatttgca ataaaaaagc gtactgctca ggatttgaat aaaagagcgg
     1561 caatacttcg ttatcttcga cattttcata aaatggttta gaatcaatct cttttgcaag
     1621 catatccgtc aaactgctct tacctgcacc aatcgttcct gaaatcaaaa tcactgacat
     1681 acaacccttc ctcttctata agtactcgcc tacgtaagtt tcaacaggca atgtttcaaa
     1741 atcatattca cgtacagctt ggtctgattt taaatacgta tggagacttt gtgtaatcat
     1801 caagttcgta ggtaccgtct cttcttgtac aatgatcatc ggttttttca acatagttgc
     1861 cacacctaat tcgtaagccg ttcctggatc gattgtttga tgctcaaagt caaggatagc
     1921 aacaacaaat tctgcatttg tcagttcttc catatctttt tcgtatactt cctgcgccca
     1981 tccagcgctg aatagttcat aattgctttc ttggtgatga cgtggagagt agaaacttgt
     2041 gactgttttg ttttcctcca acgctttttc gatacgactt actcgatcga tttgctcctc
     2101 tgaaaaaaat ggtcctgcta aatatatttg actcattatt ttgcctcctt caataataac
     2161 tgatttttaa tagatactgc tcgtaattca ttgaccagtt ccaatggatt gttggaaaag
     2221 attacttgat ccaaccattt tttatccata aaatcctctt ttgcaaaagt ttctaataat
     2281 tgttgcaagc cgctgtaata accgccaata ttcaatattc cgattggctt gtcgggcgtc
     2341 agaccgattg ctatccaaga aagaagttgt gagaattcct ccaatgtacc tagacctcca
     2401 gggaaaacga ggacagcatc tcccttttca aataataata cctttctttc atcgatcgtt
     2461 tctgtaggaa taaatgtagt aacatctttt ccaggcaatt cagtttcaaa aagcccattc
     2521 ggatagatac ctgtgacttt tccgttattt tccagtactg ttttactaat cactcccata
     2581 atcccggagt atgaaccacc gtaaaccagt tcgatttctt cttgtgccat gtattttcct
     2641 aacgtttgcg caataaattt gtaactttct ttattaccaa aacgggaacc gcaaaaaaca
     2701 gtaagtttca taaaaaaaca ctccctaact caaaagtcta tcttcattct acacgtgttt
     2761 ttttaaaata aaagactaga ttttgtagtt ttttagtggt atatagg
//
LOCUS       DAZULY010000263         2745 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000263 DAZULY010000000
VERSION     DAZULY010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.268"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            256..1227
                     /locus_tag="ACG81A_002596"
     CDS             256..1227
                     /locus_tag="ACG81A_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS5015909.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            1259..2656
                     /locus_tag="ACG81A_002597"
     CDS             1259..2656
                     /locus_tag="ACG81A_002597"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFS5015910.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
BASE COUNT          981 a          435 c          605 g          724 t
ORIGIN      
        1 tttaaaaagg caaaaaatcc gccactttca gaaagcgttt taaaaaagtg ttgcaatctt
       61 ttaaaaaaat gctatattaa aaatgaaccg gttcaaatac ttgtttaagt ataattaata
      121 gtgagaggaa gttgctctac tgaagacttt agttatatgg atattgatga caagcaacac
      181 tatgcaaaga atgcggtcat cataatcgaa caacaagaaa gagaaatcaa atgtaactgg
      241 caggagtgag aatgtatggc atcgatccgt gatgtggcta agatggctgg tgtttcagtg
      301 ggaacagttt cccgttactt gaatggtcag caactgaagg aaaagaacat gaaaaaaata
      361 gcagaagcga tttcagcgtt ggactataaa gaaaacatca ttgctaaagg gttgaagaat
      421 aatcgaagct tctctattgg acttttgata aacgggattt ccagccgttt tgggtcggaa
      481 gttgtctcag ggatcgaaag agtagctgaa gaaaatgggt atagcttatt attaagcggg
      541 tttgccgatc agccagaaaa aattgaacaa aaaattgaat acctgatgaa gcatgtgatc
      601 gatgggctga ttgtcttctt atccgaagaa gagtggtctg gatttgaaat gctcacaaaa
      661 atgagtattc cagttatcgc attgaattgt ccagaaggac cagcaggtat tgatacaata
      721 ttagtgaatg atcgagaaag tgtagcaaaa gtgatcgctc atcatggtga acaaggacat
      781 aaaaaaatcg gatttattgc tgccactcaa acagactacg tagcaagaga acgtttagca
      841 ggagccaaag aagcggtcca aaatgatcca tcgatccaat tagaggtctt tacaggagac
      901 tattctagaa aaagcggcta ttacggggca aaagcattat tagaaaaagg ggcaactgct
      961 atttttgtca gcaattacaa tatgtcgatt ggtgccatcg aatgtttcaa tcaagaaggt
     1021 gtcagaatcg ggaaggatat tgcgttcagt cactatgatt atttagataa aaacgatctg
     1081 agtatccttc cgaaaatcac gatcacgcca ccaacagata aaatcgggga acgagcagca
     1141 gaacagctgt taaaacgaat caaaaaagaa gaggaagaaa atgaaaaagt gattgtgatg
     1201 gaaaatatca tacatggaat caactaaaac gaactaaaaa agaaaaagag gtgctttgat
     1261 gaaaaaatat caatttccag caaatttttg gtggggttct gcagcaagtg gcccacaaac
     1321 agaaggaagg atttcaggcg atggaaaagg agagaacatt tttgattatt ggtacaaaaa
     1381 agaaccgcaa aagttctttg atcaagtagg acctgaaaaa acctcgcaag tttatacgaa
     1441 gtatcaagag gatgtacagt tgatgaaaca gactgggcat aattcatttc gaacatctat
     1501 ccaatggagc cgtttgatac ctgatgaatc tggaacaatc aacgaagaag ccgttcgttt
     1561 ctatcatcgt tattttgatg agttgattga aaacggtatc gaaccctttg tgaatctgta
     1621 tcatttcgat atgccgatgt atctgcaaga aaaaggcgga tggttgaacc gagaaacggt
     1681 tgaagcctat gaaaaatatg ccaaaagttg ttttgaattg tttggttcga aagtaaaaaa
     1741 atggtttact cataatgaac caatcgttcc agtcgaagca ggttatttat atcgatggca
     1801 ttatccagaa gagtcagata tgaaaaaagc gatccaagtt ggctatcatg aagcgttggc
     1861 ttcagcgtta gccatcaaat cttaccatga aatggagcaa ggtggtgtta ttggtatcat
     1921 cttgaatctg acaccaagct atccaagaga cgaatcaaac cctgaagatg ttaaagcagc
     1981 gcaaatagca gatgcgttct tcaatcgttc attccttgat ccagctgtaa aaggcgagtt
     2041 tccaagtgaa ttgattgaga tagtaaaaga acttgatctc atgcctgtga tccatgcaga
     2101 ggatttgacg gttatcaaag aaaatacagt agatctttta ggtatcaatt attaccagcc
     2161 acgccgggta aaagcaaaag aaacaccaat cgatactaaa aacggtccaa tgcctgagga
     2221 ttatttcgac aattatgaaa tgcctggtcg taaaatgaat ccttatcgcg gatgggagat
     2281 ctatgaacaa ggaatctatg atattttgat caatgtacga gatcattatg gaaatatcga
     2341 ctgctttatt tctgaaaacg gtatgggtgt agaaaacgaa gcacgattta tcaaagaaga
     2401 tggcatgatc cacgatgatt atcggatcga atttgtccaa tctcatctga aatatgtcca
     2461 tcaagccatt caagaaggtg ccaattgttt aggctatcat atgtggacat gtatggataa
     2521 ctggtcgtgg accaatgcat ataagaatcg atatggcttt atttcagtag acttagcaca
     2581 agatggaaaa agaacaatca aaaaatcggg tcattggttc aaagacgtat cagataaaaa
     2641 cggatttgag gcttaagaaa gaatagttga gagcaaatgc gtgaaattct ccattcttac
     2701 cattttgtga aaaagaagtt caattattta cgatcttttt caact
//
LOCUS       DAZULY010000264         2739 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000264 DAZULY010000000
VERSION     DAZULY010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.269"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="ACG81A_002598"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="ACG81A_002598"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFS5015911.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1072 a          425 c          558 g          684 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatata
//
LOCUS       DAZULY010000265         2728 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000265 DAZULY010000000
VERSION     DAZULY010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.270"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            174..518
                     /locus_tag="ACG81A_002599"
     CDS             174..518
                     /locus_tag="ACG81A_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFS5015912.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="ACG81A_002600"
     CDS             519..938
                     /locus_tag="ACG81A_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFS5015913.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="ACG81A_002601"
     CDS             complement(1030..1380)
                     /locus_tag="ACG81A_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015914.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2676)
                     /locus_tag="ACG81A_002602"
                     /pseudo
     CDS             complement(1346..2676)
                     /locus_tag="ACG81A_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
BASE COUNT         1011 a          491 c          527 g          699 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttg
//
LOCUS       DAZULY010000266         2698 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000266 DAZULY010000000
VERSION     DAZULY010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.271"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(642..1145)
                     /locus_tag="ACG81A_002603"
     CDS             complement(642..1145)
                     /locus_tag="ACG81A_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFS5015915.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1504..2529
                     /locus_tag="ACG81A_002604"
     CDS             1504..2529
                     /locus_tag="ACG81A_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS5015916.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
BASE COUNT          883 a          412 c          497 g          906 t
ORIGIN      
        1 aaaaattagt caaagaaggg cgtatagatt tcttttgata ggagatatac aacagaaatg
       61 aataaatatt aacgtgtttt caaacagtat aaaacggtgt ggtttttgga ataaaaaaat
      121 aacatatctc attttacgct tttgttattt tatacttaaa aaataacgac acagaaaata
      181 ccgtgtctca gatttctcta ctacttctaa aaactactgt ttttttcctt tgaaatgcct
      241 tcaaaaagta ggatcaggca gaatatactg gcagaatcaa ggagaaggga gcctaaataa
      301 ggagaaaaac catagaaaat ataaagtaat tacttataaa acaagctttt tatagaaaaa
      361 taatatacct gagtagggta taaattaaga cggaattcct ttctagattg agatattgac
      421 taggaagaat gttaagattt taagccttta agctaaaaaa aaaccctttt tgttaaataa
      481 ggggaatata gcctgtcagt ttcaaactgc aagtcgtcct gctgcaataa tatagtagat
      541 tagagatgaa aatagatttt tttaaaccaa tgactgaata aaaagaccgt catagataca
      601 cattggaatg tgtctattac ggtctttttt tacgaacgat tttattgaag acggacagtt
      661 cgggtcaaaa ataaaaaagc tacgatcaga aataagaata aaatgtatcc caaaataatg
      721 gctagacggt ctaacttatt tgtcgataga tgtttgttta gaagttgata ggcccataaa
      781 cctatcagtc ctcctgttgt attagttatc aaatcagtga tgtccgctac gccaataccg
      841 aataggaatt gaatcaattc tgccaagaag cttacaagga aaataatccc taatttttta
      901 gaaaaagaaa gtctcttgaa attgacattc aaaagcaccc caaaaggaaa aaagacaatc
      961 aagttataag tggtctcacg taataccgtc gtattttcaa aagaaaaagt agaaaaaggg
     1021 acgagattga cattgctgta agaataagtg agaaattgtg gaagattaaa tgatagcttg
     1081 aataaaatca accataatag aaagaataaa taaatcccta ataaaaattt tgatacatac
     1141 cgcatagaca tctcctccat ttaaaaagta taccatcgaa aaccgctgaa aaatcataaa
     1201 aaagtatgaa gtttaaggac caacgtagtt atcgtacgaa aattagaagc ttttttttgt
     1261 tgggaatccg gtttctttaa caaagctaag atagcgagtt gcttggaaaa gttgctatct
     1321 tttttctgtc aaaacgactc ggaatgttcg ttttttgttc taatttctcc aagttgttta
     1381 ctttttgata ctaagtttat gaaaaatcaa gaaatagttg ttatactagg taatgctgac
     1441 aaacgggatt ggcatttcat cttggttcag ctttttttcc aattactagg aaggagggat
     1501 agattgttca agacttattt tcgtttcaat aaaatacaag ataatccttt tcgtatcatg
     1561 aatctgatgc tacttatctt tattgtatta tggttaggtt acataaatca tgattttttg
     1621 atttcaagtt tttcatcatt agggatcttt acttttttct attatcaaaa cataccgatg
     1681 aaatatttgc tgaaacgtat gtttctgatc ggttgcggac tgttggttgc tttcgttttg
     1741 gggatcttat cgacttatgt tttatggctt gaaccatttg ccgtagcttt ggttgctttt
     1801 tccagcagat tcgtgttgcg attattccat atttctaaac caggtggctt gttttttgcg
     1861 atgctttctg ccatgggaac gagcatgcag ctgccaatcg ctcaattacc gatcgtctca
     1921 ctttattttt ttatgggtgt cgtttttgcc ctgattgcgg cagtgattac aaaattactg
     1981 gattcacggc ctgagcagac aattgaaaaa gcaactttaa aagaaagatt tcacgaagag
     2041 cctttagtga tcattgattc tgttttctac agcgctgcct tatttctttc cgtttatgtc
     2101 agtcacggct tgaatctgca taatccttat tggttgacct tgtcatgtgc atcgattttg
     2161 ctagcagaaa acttggatgc gatgaagcat cgacaagtcc aatatctgat tggatcgatg
     2221 ggtgggttat gtgtgtcggc attcttgtca tttgtgccgt ttacgcagtt gcagaccatt
     2281 tttctgatca cttttctata tggaatcgca caatttctag ttgctagaaa ttatgcagtt
     2341 gctaatattt ttcttaatcc gatggcgttg atgctatcta cacttattcg tggagcatac
     2401 ttgatttctt tgatcgaata tcgtttttta gggatcgtca taggaagttt catcggttta
     2461 ggtgttgcgt gggtaatgac ggttggtttg caacattatt taacggttgt tcgtcaaaaa
     2521 ttagaataga aggacaaata tatgtgccaa aagaggttat gacaagattt ttgtcacaac
     2581 ctctaattca aggtgttcaa aagccagatt ctcagcatta agtcaaattc acacaaaaat
     2641 gtggaaagga actttcaaaa aaattttata tttattctat caaaaaattt tgtttatg
//
LOCUS       DAZULY010000267         2636 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000267 DAZULY010000000
VERSION     DAZULY010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.272"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1057..1722)
                     /locus_tag="ACG81A_002605"
     CDS             complement(1057..1722)
                     /locus_tag="ACG81A_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5015917.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1848..>2636
                     /locus_tag="ACG81A_002606"
     CDS             1848..>2636
                     /locus_tag="ACG81A_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFS5015918.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMY"
BASE COUNT          850 a          413 c          471 g          902 t
ORIGIN      
        1 ggctaggaac ctgataacat caaggttcct agccttttta taataaaatg aagcgaaaat
       61 aatttctttt aaagagccga gattatcagc tatataagag tgaatttgat gcaaaaaaca
      121 gagccgaaac agtcttggtg ttcgtagctc tgcttatatg tttcgaattt aagacaaaga
      181 gttggcaaaa gatgttgata aggaagataa gataactgtg ttatataaac taggtaacta
      241 actgtaacaa gctaatttta agagattctt attagaagac gtttaggaat ataactaaga
      301 aaatgattat acaggtaatt gtcatttcaa taatgttttc taattttcgc gtctttgttt
      361 taagaaaaag gccagaatca gttggaaaaa agttcgtacg atagtgaaga ggggcaaaat
      421 ttcctactaa tattcccact aacaagagat aaaacggcca ggttggtgtg attcctctat
      481 aaaaaaatgc ccatgctacc gaaatcccat agattaacaa ctgaacaatt tttacaatga
      541 aaaaattgtt ctgccattac tcaaagtctc ttcctccttg tatcgtttat ttgtttcatt
      601 ttatcattat gataggcgct tgaacaatcc attttgtatt ttattgagta ctcttggata
      661 aactaccagc gattatcata tttatttatg ttactgtaag ccatcatata aaagtaaatg
      721 agcagatcat agaaatataa aaaagaacta aaattgatct aaattatgtg cagcttgact
      781 acgtaccaat ctatattact tactagattg gtcgttaatc tattcggatc gaatgagcaa
      841 aatatttttg tgaatgtcca tccttgatta tgattccaaa aagaatcatt gggaggctgt
      901 ggcgtaaata ttatactata agccagaaat acctatcatt ctgaactttt ttatttagga
      961 actttcatct caaggctttt tcaattaatt tgtacccctg ttttttggat aagatcatgt
     1021 gcattgattt tttctatctc tacgttcatc tcctacttaa tgcctatctg gctcctgagg
     1081 gcttcttttt aaggcgtcgt catagtcttg gctgaggatt tcttggtaat ctgagggaat
     1141 ggcttcccaa gtgagcaggc gaacgttcat gacgtgtttt cctttgtgtt tgattacagc
     1201 atggtttttc tctttttcta cctctccagc gtgaaaagac agccatgtac catggccaaa
     1261 gatatcatta catacccaac gttcgatgga attgtccacg tatcctacag ggagtgtggt
     1321 atagccgatt tgtggtttca aaaaaggatt gaatcgatcg atcgtttcat ttttgtattg
     1381 ataattgata agaaaaagat agaccaaaca aaagacgagc agaaagaaag atagtgtctt
     1441 tttgagtgag aagggttgtt ttgatgaata agatttcttg ttaacaagag gttcatctgg
     1501 caatgttggt tctattgatt cggtagctga ttcaatagaa ggaaaatcaa tcggcagacc
     1561 taaagtatga tgtaaaagtg acagcatttc aaggtctggc attgtacgag aattttccca
     1621 actagaaatc gttttatcgg aaacaccaat tttttcgcct aattcttttt gcgtaagctt
     1681 acaatttctg cggtaagttc gtattttttc tccggtgttc atcttcttct cctttcgatt
     1741 ctacagtata tagatttatt ctacatgacc tatacacttg atagatacgt ttttttgatg
     1801 aaaaacggta tgattttatc aaagaatcat ttaagtaagg gagagtggtg ataatcaaaa
     1861 aaaagttcac agttttactc acaatcatag tgaccggtat catgagtggt tttgccttgg
     1921 ttagtataaa taaacaggta ctctatcgga caaatgaatt tttccatcaa acagatactt
     1981 atactccgat tcgattgcca caacatttta ttgggcaaga agaatatgag cagctaatca
     2041 tggtatttga aaatgtctca tcagaaactg agctttctta tatgaaaaaa acagtaaatg
     2101 aatccaatga agtagataca tcttggaact tcaagataca gccaaagctt gaaatgacat
     2161 tgtattatca aggggatgtc aaatcttctc acgagaaggt accctattct aatacctcct
     2221 ttcgcgtaac gtccattgaa gaatcgatca aaataaaaga ttttgaaggt gaatttttct
     2281 tgatgacgag agaaaaagaa gcctatgaac ggttcatttc actattcact gatcgattca
     2341 atcaagcatt tcacactgat tatgccacta atatttttga agactttgaa aatagctatt
     2401 cgtattcggt tatttcaaat acagacgtat ataattatcg agtgtattta actattggat
     2461 tgattctttt attccttttg ttatcatcct ttttcttatt gatgaataaa gaaatcagtt
     2521 tattgttgaa taatgacttt tcattacatc aagtattgtg gtgtttattg gggaaaaaga
     2581 aaatcattat tacattcata attgcaggaa tcattggtgg ttttattttt atgtat
//
LOCUS       DAZULY010000268         2601 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000268 DAZULY010000000
VERSION     DAZULY010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.273"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(203..1255)
                     /locus_tag="ACG81A_002607"
     CDS             complement(203..1255)
                     /locus_tag="ACG81A_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5015919.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(1268..2206)
                     /locus_tag="ACG81A_002608"
     CDS             complement(1268..2206)
                     /locus_tag="ACG81A_002608"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS5015920.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT          852 a          556 c          398 g          795 t
ORIGIN      
        1 acacatattt ttattctagt tgtcttttga acaatcaata acataaaggt gagtttcttc
       61 tacattttaa aaaatcgaat ggcactgaag agttcagttg tcagcatcaa tctcctactc
      121 aaaaaggcaa aaaaacgtaa gtcagatgtt gttgacatct cacttacgca tccattgatt
      181 gaaaatttct tagtaaaatc tactagccta aaaatttgaa gacttcttct cgatcataca
      241 gtttaaatac tttctcgcat aaaaattgag taattagtgc cgcaaaaaca gggatgacta
      301 accaacaaag aacaacaaga aagccattta gccctgcatc taaagaagcc aacggaccaa
      361 caagtccgac cacgccgaaa ccagcagaag caggcgtacc tgaaatagaa aatagagcga
      421 caggaatagc tgtaatcact gcactaacta aacaaggaat aagaatcaca ggttttctaa
      481 atagattagg catcatcatc ttcatagcgc ctaaagcaat cgcaattgtc acacctgact
      541 tattaacctt ccaagagttc actactaaaa caaaagtggt tgcagctacg cccatcgcag
      601 ccgcaccagc agaaacacca tctagttgga ttgccattcc gattgctact gttgaaatag
      661 gagagataat caaaaaggca aaggaacaac caatcaagat agacattaac agcggttgta
      721 aggtagtgaa gttattaatc aatgatccaa ttgcagaagt aattgctgtt acaaaaggga
      781 ataaataaaa accaagtaaa cctgcaccta cacctacaac aatcggtgtt aacacaatcg
      841 acactgaccc aaaactattt ccaatcagta aaatcagccc gacagcaatc gatgctgtca
      901 gcatcgtatt aataatatcg cctgtaccag caccaacgta aacacccgtt tcacccaaat
      961 caggtgtgaa tttgactaca ccagaaccaa caaaagaagc tccggctaca accatcattc
     1021 tcatcggatc aaatccaaat tgtaaggcaa tcaaaccacc aatgatcaaa ggtgttgcta
     1081 gttgaaaaat aactgctaac tgtgcgatcg ttaccgcaaa gggatacgca ctaaaatatt
     1141 ttaaaatggc acctaatact gcattaggaa tcaatccaat aatgattcct gttgctgtac
     1201 cagcgaggac tttattcaga aaaattttcg gtgatatttt ttgtgaagtt tccattcaat
     1261 ctctccttta ttttacgttc aaactatctt cttttgcatg gacaagctca gtcaagaaat
     1321 cacaataagg cgtcgcaaca ccatatttct tcccttttct agaaattgcg ccattgatat
     1381 aatcgatttc tgttaatcga tggttcttaa tcaagtcttg atacattgaa ggataatgta
     1441 acccaattgt ttccgggtca aaacaagatt cacaatgtgc aatgacttct gggacatcta
     1501 gttcaatctt ctctactgct gctactttgg caaactcatt gacaatcgtc gccaccattt
     1561 tatgagcagt cgatgttttt cctagctcag ccatattaac gtctaagatt gtgcatagtc
     1621 cgttcattgt tccgttcaca catgctttgc gataaataga atagtgaata ttgtcagaaa
     1681 aatgagcatt taatcctgat tcagacaatt tgtctgctaa tttttttgca gctgcttctt
     1741 ttccatcacc taaattttgt aattctactg aaccacttcc aaataatttg acctgaccag
     1801 gaccttcaag acctgccgtc cacatggtat ttccaatata gatattttcc attggtacaa
     1861 atttctcaat aatatcttca tgcccgatac catttaatag acataagacc tctgtatctt
     1921 ttttgattaa tgattggata tcctgcagca ttttttccag ctgcatcgct ttggtaaata
     1981 gaataatcag atcaacttga tcttcttttt ctacttcgga ttgaagaacg attggtagtt
     2041 ttgcttctac ctctttacca ttaaaatttg cttgtaatcc atgttctttg atttgttgga
     2101 catgttctgc ccagccatcg attaataata cttcgttccc actttggtga agcatcaacc
     2161 caaagcgact tcccattgcg ccagcgcctg caattgctat tttcattgat tcttcctcct
     2221 ttttctttct attatactca tttgagaatc ttttcacaac ctcgttttgt tattcttctc
     2281 ttatttatca tgtttatatc caccattcaa gttattttat cagcaaatca ataaaaactg
     2341 gaatagaatc ccaatttcat cgtaagagaa actagactag caataagaaa atagaaacat
     2401 tcatagaaaa aatctcactc ctctgattat aaaagagggg gcattaacct cgtgtatact
     2461 aaaaccttcc attcattcga tggatgattt tctttttgtg aaaaaatatt cattgtagta
     2521 cgatgttgcg caacacagac ggatggtatt gcttattatt tattaaaatc gaacaataca
     2581 cttatttaaa aacttctttc c
//
LOCUS       DAZULY010000269         2590 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000269 DAZULY010000000
VERSION     DAZULY010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.274"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            16..2430
                     /gene="leuS"
                     /locus_tag="ACG81A_002609"
     CDS             16..2430
                     /gene="leuS"
                     /locus_tag="ACG81A_002609"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFS5015921.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
BASE COUNT          882 a          473 c          570 g          665 t
ORIGIN      
        1 acaggaggac agcaaatgag ttacaatcat aaagaaattg agaaaaaatg gcaaaaatac
       61 tgggcaaaaa cgaatacttt caacacccat gatgatcctg aaaagccaaa attttacgca
      121 ctggatatgt ttccctatcc atccggtcaa gggctacatg tagggcatcc tgaaggttat
      181 acggcaacgg atatcctttc acgtgtgaaa agaagtcaag gatacaacgt gctgcatcca
      241 atggggtggg atgcattcgg tctgcctgct gaacaatatg cgctagatac aggaaacgat
      301 ccagctgagt ttacccaaaa aaatatcgaa acattccgac gccaaatcaa ttctttagga
      361 tttagttatg actggaatcg cgaagtgaat acaactgatc cggagtatta taaatggaca
      421 caatggatct ttacgaagct ttatgaaaaa ggtctagctt atgaagcaga agtagcggta
      481 aactgggtac ctgaattagg aacagttatt tcaaatgaag aagtgatcga tgggaaaagt
      541 gaacgtggtg gctatgatgt agtcagaaaa ccaatgcgcc aatggatgct gaaaatcact
      601 gcttatgctg atcgcttatt ggatgatcta gaattagttg attggccaga aagcattaaa
      661 gaaatgcaaa gaaattggat cggtcgttca gtaggagcaa atattgaatt taaagtggct
      721 ggtacggata aatcctatac cgtatttacg acacgaccag atactctatt cggtgcaact
      781 tactcggttc ttgcacctga actagatctt gttagagaaa ttacgacccc tgaacaaaaa
      841 gctgctgtag aagcttatat cgaagagaca gctaaaaaat cagatctaaa gagaacggat
      901 cttgcaaaag aaaaaacagg agtatttact ggtgcttatg caatcaatcc agttaatggc
      961 aaggaaatcc caatctggat cgccgattat gtattatctt catacggcac aggagccatc
     1021 atggctgttc cagcacatga tgaacgtgac tttgaattcg ctcaaacttt cggactagaa
     1081 attcttccag ttatcgaagg tggagatgtt gaaaaagctg cttacacaga agatggtacg
     1141 cacatcaact ctgaatttct agacggcatg aacaaacaag aagcaatcga taaaatgaat
     1201 gaatggctag aagaaaatgg cgtagggaaa aaagaagtca gctatcgctt acgtgactgg
     1261 ctattctccc gtcaacgtta ctggggtgaa ccaatcccaa tcatccattg ggaggacgga
     1321 acagtcacag ctgtcccaga agaagagcta ccattacggt tgcctaagac aaaaaacatc
     1381 aagccaagcg gtactggaga atctccatta gcaaatatcg aggagtgggt caatgtcgtt
     1441 gatcctgtaa caggtaaaaa aggccgtcgc gaaacgaata caatgccaca atgggcagga
     1501 agctcatggt attacctgcg ttatatcgac cctcataaca aaaaagaact agcagattat
     1561 gaaaagttga agcgttggct accagttgac atctacattg gtggagcaga acatgcggta
     1621 cttcatttac tatatgcacg attctggcat aaattcttat acgatatcgg tgttgttcct
     1681 acaaaagaac cattccaaaa actatacaat caaggaatga tcttaggcga aaacaacgaa
     1741 aaaatgtcaa aatctcgtgg taatgtagtc aatcctgatg atgtggtcga aacttacggt
     1801 gcagatacac tgcgcatgta cgaaatgttc atggggccat tagatgcttc gattgcttgg
     1861 agcgaaaatg gcttagaagg aagccgtaaa ttcttggatc gtgtatggcg tttgatcgtt
     1921 gatgagaaca ataaaatgcg tgaccgtatc actacattga atgatggcaa gcttgataaa
     1981 gtgtatcatc aaacagtgaa aaaagtgaca gaagactatg aaaatcttca tttcaataca
     2041 gcaatctccc aattgatggt ctttatcaat gaagcctata aagtggacgc tctgccttat
     2101 gaatatatcg aaggatttgt tcaactactc gcaccgattg ctcctcacat gggtgaagaa
     2161 ctatgggcaa tccttggaaa tgatggtggt atttcttacg caccatggcc aacatacgat
     2221 gaagcagcac ttgttgaaga cgaagtagaa gttgttttcc aagtaaacgg taaagttcgt
     2281 gcgaaaagca atgttccccg agacttagga aaagatgagc tagaaaaagc agcattagcc
     2341 aatgaaatag tgcaagaata tattgaaggc aaaacagtca gaaaagttat tgcggtacca
     2401 aacaagttag tcaatatcgt tgctaattaa ttggctcttt gtcaataagg actgatgagt
     2461 tgtgcaaaat caaatctggg ccagaatgaa cttcattctg tctcagattt tttgttattt
     2521 caaattgatt aattgattga tcaaaaaggt tttgattttc atgttctcaa acacgttcaa
     2581 tgtctttttc
//
LOCUS       DAZULY010000270         2566 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000270 DAZULY010000000
VERSION     DAZULY010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.275"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..379)
                     /locus_tag="ACG81A_002610"
     CDS             complement(<1..379)
                     /locus_tag="ACG81A_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFS5015922.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPS"
     gene            565..1968
                     /locus_tag="ACG81A_002611"
     CDS             565..1968
                     /locus_tag="ACG81A_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HFS5015923.1"
                     /translation="MTASDLIRNFIMMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDF
                     FRYYLAAITGEYSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPE
                     GKYSEVLSDFQSMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNG
                     DDTSRVSKAFPSYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNL
                     LNANAYAMRLARWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYT
                     GLVNTIGNLTLVTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISAR
                     SEALINELITMYPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYS
                     KIKDIASDSLDETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDY
                     WKYDNGISINDSLRNKK"
     gene            1989..2273
                     /locus_tag="ACG81A_002612"
     CDS             1989..2273
                     /locus_tag="ACG81A_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcQ family protein"
                     /protein_id="HFS5015924.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          873 a          384 c          526 g          783 t
ORIGIN      
        1 gactaggaaa actatctata attccgacta gttcaccttt ggtgagcttc ttgatgaaaa
       61 agtatcgaat aatcttgata gtgtaagcta agtttgaact taagcgagtg aatcgactcc
      121 tactaggaaa gtcaccatta agaaataaga cggaacaaac cgctctatac gtggctcttt
      181 ggctagtata gccattaatt atgccccata taacacaagc aataatcact gtatcgcgtt
      241 gcttcagttg atcagtattt cttcggtttt taatcttatc tggtacacaa ttgtggtata
      301 tgttagaaac agttctcata atttccttga atgttataaa agtatctgta taatgttcag
      361 tatattttga tgaacgcata tgtagtcctc atttcttggg agttttgaga ctactatatg
      421 cgttttttgt tgatttacca acatgtttag acattttcct ctaggaaaaa attaactagc
      481 accacgcgta ttgtttatat taaattagat gaccgtgata atgcacagca aatttttgaa
      541 agtatcaatt ctaccggtga aagattgact gcctcggatt tgattcgaaa cttcatcatg
      601 atggataaat ctaatgaaga gcagactacg ctttatacta aatattggcg taggttggaa
      661 gaagtctttg ataattctaa aggaatggaa gactttttca gatactacct tgctgcaata
      721 actggggagt attcagcaaa gcatattctt tatcaagcat tcaaagacta ttggcataaa
      781 gagcgcgatg tatcaagtga tgccgaattg ctccaaaaat tagtgcgata tgctggttat
      841 tttgcgagac tttactataa caaacctgaa ggaaaatact cagaagtatt gagcgatttc
      901 caaagtatgg aaagtatgat gcctgcacct tttgttttgg gattatctga atggtactat
      961 catgaccaat ttatcactga agaccaatat attgatgcga ttaaggtagt gaatgattat
     1021 cagcttagga gatattttaa tggggatgat acaagcagag tgtcaaaagc atttccttca
     1081 tatttgaaaa gtgttaggaa gtacgctaaa gctaatggat atgaaaatat cgttgatatt
     1141 gtaatttatg ttcttgttac tagaaatcaa tcaaataata tggcacttcc atctgacaag
     1201 gcattgaaat ctaatctatt gaatgccaat gcttatgcga tgagattggc ccgttggtta
     1261 cttgaaaaaa tagagaatcg agaaaattca gcaacacttg atatgagtaa cttgtccatt
     1321 gaacacatca tgccacagac tagtacttca tattgggaag agaaggctgg tacatcgggg
     1381 gaagaataca caggattagt aaataccatt ggtaatttaa cactcgttac aaatcctgat
     1441 aattcggccg cgggaaataa agactttgag accaaaaaga aaatctttga agataccctt
     1501 catattcgta tgaataaaga tttgtacgaa ttgactgagt ggacatcatc ggatatttct
     1561 gccagaagtg aagctctaat caatgaactg attactatgt atccctattt gagatcgtcg
     1621 ggcgactatg aacacgatgg gaatagggaa attttcttag aggcgcaagg aataaaagca
     1681 actgggtatt tgaacgaaga cgagaccgtg attatacatt caggttcaga aatttattct
     1741 aagattaaag atattgcatc tgattcgcta gatgaaactc gccaagagct gttggataat
     1801 ggaatcattg aagaaacaat tgggggacta caatttgttc aagattacac tgcctcatct
     1861 gtttcgaatg cagcagctct gctattaggg ggtagccgga atggttggga ctactggaaa
     1921 tatgataacg gaataagtat taatgattcg ctaagaaata agaaatagag catagaggtg
     1981 gtgttaggtt ggataagaac aaactaagat atgcaattct taaattgcgt gatgaaaaaa
     2041 agaatccttt tattgagctt aaagggcaag ttccagaaga agatatttta gaacaaacta
     2101 aatttctttc gcgcaatggc ttactagaat ccatggtttg ggcggataac actgttcata
     2161 tctgggggag tgtttctctg gaaggggaaa aatatttagt tgaaaactca ggtcttgcta
     2221 aggcgtatgc attggcaaaa gaagttaaaa attggattcc attttggggc taagataagt
     2281 ttttaaacgg agaaggtagc aaatcataat tggtttgttg ccttttttga ttgcccaaaa
     2341 agaaaaatga caaagtatag agataaatga catagtacac gggaaaaatg acattgtatc
     2401 accgacaacg tctaaataga tgttgatata tatacgttcc gtacaaataa ttataaaaat
     2461 ttaaatgatt tgagtagaag tatcagtgaa tatctcaagg ggaatagcta tctcaagatt
     2521 cgattttcac atattattat ttgtcaaatt aagcgagaca aaactt
//
LOCUS       DAZULY010000271         2559 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000271 DAZULY010000000
VERSION     DAZULY010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.276"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..2543
                     /locus_tag="ACG81A_002613"
     CDS             102..2543
                     /locus_tag="ACG81A_002613"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFS5015925.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT          855 a          455 c          606 g          643 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatcttta
//
LOCUS       DAZULY010000272         2549 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000272 DAZULY010000000
VERSION     DAZULY010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.277"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     misc_binding    complement(83..289)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(359..2377)
                     /locus_tag="ACG81A_002614"
     CDS             complement(359..2377)
                     /locus_tag="ACG81A_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFS5015926.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
BASE COUNT          656 a          525 c          476 g          892 t
ORIGIN      
        1 ctgtttattt ttatttcctt ttgctttgtt ttctcaaact tcacaaaaga aataaaaaaa
       61 tcctataaaa agattgctct ttttatagga cgtaatctac gcggtaccac ctaattttac
      121 cttagtgaaa aggcctcgaa cgttgtaacg gccgtcaccg ccgcttgctt actcgggtat
      181 tcagcaaggg caactcaaag gcgagttcaa aagcatccgt gcacattctc accagccatg
      241 cgctctctga aacttcagtc tttttactac ttccttttca agtgttgtca tatttaagta
      301 gccttatagt aacaaatatt tttgtggaat tcaatcgtta tttcttcgaa cagctatttt
      361 atttagctac aatcaacgtt tgacctggat aaataaagtt gttcttaata ttgttccatt
      421 cgattagttg agccattgta attccatgac tgtcagcaac actccaaaca gattctccag
      481 atttcacagt ataggttttc gaaccagtaa ctgattgttg gtttgttgat tgagatgaac
      541 ctttcttcac aatcacttgc tgtcctggat agatgaaatt atttttgata ttattccatt
      601 cgattaactg atccatcgtg atatgatgtt tgttcgctac gctccaaact gactcgcccg
      661 ctttcacggt atacatcgta ttaccagtgg atgatgtact tgtcgtagca gacgtattcg
      721 gtgtatttgg tttcgctgca gaagtagttg aacctgtatt tgtatttgcc gaagttccac
      781 ctttaacgat caattgctgt cctggataaa tgaagttgtt tttgatatta ttccattcga
      841 ttaactgatc catcgtgatg ccgtttttat ttgccacgct ccaaacagat tcacctgctt
      901 tcacggtata agtgccttga gacgaactgc tgccagtatt aactgaactg ctgttatttg
      961 tattggttga cgaacctgta ttacctgacg tgctgtttgt tgatccactg tttgaaacag
     1021 tcagttgttg tcctgggtag ataaagttat tttggatatt gttccattgg attagctgag
     1081 ccatcgtaat tccgtattta tttgagacgc tccaaacaga gtctccggct ttaactgtat
     1141 atttcgttcc tgaagctgta ttagaagaac ttgagctgga cgaactagag ccacttgtat
     1201 tcccagtatt tgagccagta tttgctccag cattagaaga accgccattt gatacgatta
     1261 gctgttgtcc tggataaacg aaattgttct tgatattgtt ccattcaatc aattgagcca
     1321 tcgtaattcc gtgatcattt gagatcttcc aaacagaatc accagattga acagtgtatt
     1381 tcgcaccagt tgtttgtcct ccgtttgatg taccagcatt tcctgaagaa ttggtattgt
     1441 taccggaatt attcgtcgaa gagccatttt cctggctacc tttgattgtc agtttttgtc
     1501 ctggataaat gaaattgttt tggatgttgt tccattggat caattgatcc attgtgatac
     1561 cgaatttatt tgagattccc caaacagaat caccagattg gacagtataa gtggtcgaag
     1621 ttgctgaacc tgagtttgta ttaccagaat tgcttgaacc gccagtacta cctgcacttc
     1681 cagtattatt gccacctgta gtcgctccac cgcctgcgtt tccgctagct ggtgtatcat
     1741 attgggtcaa tccgtaagtt gcgatgatat tatttaattt gcctgcgtaa ccaggatcag
     1801 tcgcataacg ccctgtcaaa caagctgtcg catctgtata ggatttcgtg ttgctcttcc
     1861 aagtaccggc atagtaataa ccgttgccaa atgaagtatt tctaagtaca taggcattgt
     1921 cattgaaaga ttcagcaaaa gaaggatatt gtcggaaagg ctccttttta tttacccatt
     1981 gattatttaa aaattcaagc gtatccatgt agacggtttg tccttgatag ctgcctttta
     2041 tcccaaaaag attataataa ggtgcttgag ataacgcact actgccccaa ccgctttcta
     2101 caactgcttg tgcgatcatc actgatgcat atagatcgtt ggcttgagcg attggttgag
     2161 cataagtagc aatttctgtg ataaatgccg ctgtattgat tcttgcgccg aactgaacag
     2221 gtgtttcatc cgcttgaaca ggaaccggct gtactaatgg ggcagcgaca gaacagccta
     2281 ccatcgcagt tcccacgatt gctgcccctc gttttgcttt tcgaaaacgt ttggtctgtt
     2341 ctaaacgtct ccgttctttt cttgcaatgt tttccatctt ctatcctcca acttcaattt
     2401 atctattatt aataattata acttctctta atatttatta aaagttattt aagagctttg
     2461 ttgttttttg taatcatatt gtaatttacc gtaatgaaca acttctgttc attttcaagt
     2521 tcttcctacg tctacgttct tgtttaaaa
//
LOCUS       DAZULY010000273         2465 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000273 DAZULY010000000
VERSION     DAZULY010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.278"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            332..1441
                     /gene="pdhA"
                     /locus_tag="ACG81A_002615"
     CDS             332..1441
                     /gene="pdhA"
                     /locus_tag="ACG81A_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFS5015927.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1444..2421
                     /locus_tag="ACG81A_002616"
     CDS             1444..2421
                     /locus_tag="ACG81A_002616"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFS5015928.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT          819 a          460 c          521 g          665 t
ORIGIN      
        1 atgtgtaaat aaaataaatg tttgcaacaa aactgtataa caccaacgtt tttaaataga
       61 aacagatggt tgtaagtttg attagaaaat agaaaaaatg cagatgaaaa ataaaacgct
      121 ttccttattg tgattttatt cactaatgct gttaatatgc ggattggcat aatttccttt
      181 catcgtgaaa atttgtgctt ttagcttgta tttctcataa atccatgata atttattaat
      241 gaagacggtt aaatagtgat acagttttat tcactgtttc aatcgcagaa tgaagagatt
      301 ttcttataga tgagagagga atgtgtgaca aatggcaaac aaaaaagcac cgatcgactt
      361 ccaagcactg ctagatgaag tcaatgcaga tttcccagta tatcaagcac ttgatcaaga
      421 tggaaacgta gttaacaaag atttagtacc tgatttatcc gatgatgaac tcgttgaatt
      481 aatgactcat atggtatggt ctcgtgtatt agaccaacgt tcaacagcgc tgaaccgcca
      541 aggacgccta ggcttttttg caccaactgc tggacaagag gcaagtcagc tggctagtgc
      601 ttacgcattt gataaagaag atgttcttct tccaggttat cgggatgtac ctcaactagt
      661 aaaacatggt ttacctttat ctcaagcttt tttatggtct cgaggacatg ctgcaggtaa
      721 ttcttatccg gaagaattga aagctctacc accacaaatt atcatcggtg cacaatatgt
      781 acaagcagcc ggtgttgcat taggtttgaa gaaacgaaac aagaaaaatg tcgtcttcac
      841 ttatactggt gatggcggtt catcacaagg tgatttttac gaagcaatca atttcgctgg
      901 cgcttaccat gcaaatgccg tattttatat ccaaaacaac gggtatgcga tctctacacc
      961 tcgtgaaaaa caaactgcag caaaaacact tgcacaaaaa gctgtcgcag ctgggattcc
     1021 tggtattcaa gtcgatggaa tggatccgct tgcagtatat actgtatcta aaatggcacg
     1081 ggaatgggcg atcagtggaa atggaccagt cctaatcgaa acactgactt atcgttatgg
     1141 accacataca ctatctggtg acgatccaac ccgctaccgt acaaaagaca cagatgacga
     1201 ttggcagaaa aaagatccat tgatccgctt ccgtaaatat ttgacggaaa aaggactttg
     1261 gtctgaagaa aaagaagaac aagtcatcga agcaactaaa gaagaaatca aagcagctat
     1321 tgctgaagcc gacaaagtac caaaacaaaa ggtttcagat ttcttgaaaa acatgttcga
     1381 agtatctcca caatcaatca aagaacaaat tgcaatttat gaagcgaagg agtcgaaata
     1441 atcatggcac aaaaaacaat gatccaagca atcacagatg ccttagcact tgaactagaa
     1501 aatgacgaga atgtcgttgt ttttggtgaa gatgttggga aaaacggagg agttttccgg
     1561 gcaactgaag gattgcagga aaagttcgga gaagaccgag tattcgatac tcctttagct
     1621 gaatctggaa tcgcaggatt gagtttcggt cttgcgttag aaggatttcg tccagttcct
     1681 gaaatccaat tctttggttt tattttcgaa gcaatggacg aagtagtagc tcaaatggct
     1741 cgtacaagat atcgaatgag cggcactaga aatctaccca tcacaattcg ttcgccattt
     1801 ggtggcggtg ttcatacacc agaattgcat tcggataatt tagaaggttt gatcgctcag
     1861 tctcctggta ttcgagtagt gattccttcc aacccttatg atgcaaaagg actattgatt
     1921 tcttctatta gaagtaacga tccggttgtt ttcctagaac acatgaaact atatcgctca
     1981 ttccgagaag aagtgccgga tgaagcttac gaagttcctt tggataaagc agcagttact
     2041 cgtgagggaa cagatgtatc tattatcaca tacggagcaa tggtgcgtga agcaatcaaa
     2101 gcagcagaca atcttgcaaa agaaaatata tctgttgaaa tcattgatct tcgtacagtt
     2161 gcgccattag atgtagaaac gatcattcaa tccgtggaaa aaactggtcg cgtagttgtt
     2221 gtccaagaag cacaacgaca agcgggcgtt gccgcgcaag tagtttctga gatctcagaa
     2281 cgcgctatcc tttcattaga agccccaatc ggacgagttt ctgcacctga tacagtcttc
     2341 ccatttggac aagcagaaaa tgtctggttg ccaaatgcaa aagatatcga agacaaagta
     2401 aaagaaatcg cggaatttta agaaaagaag ggacgaaaca aagtcatggc ttatcaattt
     2461 aaatt
//
LOCUS       DAZULY010000274         2446 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000274 DAZULY010000000
VERSION     DAZULY010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.279"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(262..1593)
                     /locus_tag="ACG81A_002617"
     CDS             complement(262..1593)
                     /locus_tag="ACG81A_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS5015929.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(1742..2446)
                     /locus_tag="ACG81A_002618"
     CDS             complement(1742..2446)
                     /locus_tag="ACG81A_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5015930.1"
                     /translation="MAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKLTVK
                     KGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTFSIE
                     LPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKSVYA
                     YITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEYSVS
                     RHLYQDFVFEAVFVEK"
BASE COUNT          825 a          450 c          341 g          830 t
ORIGIN      
        1 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
       61 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
      121 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
      181 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
      241 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
      301 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
      361 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
      421 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
      481 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
      541 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
      601 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
      661 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
      721 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
      781 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
      841 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
      901 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
      961 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     1021 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     1081 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     1141 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     1201 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     1261 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     1321 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     1381 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     1441 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     1501 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     1561 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     1621 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     1681 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     1741 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     1801 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     1861 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     1921 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     1981 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     2041 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     2101 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     2161 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     2221 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     2281 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     2341 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     2401 caattttttt ctttatatct aagtaaattt ccatatactt cgcaat
//
LOCUS       DAZULY010000275         2438 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000275 DAZULY010000000
VERSION     DAZULY010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.280"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..602
                     /locus_tag="ACG81A_002619"
     CDS             <1..602
                     /locus_tag="ACG81A_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HFS5015931.1"
                     /translation="DQAITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNR
                     PQAIDLLKQILSAKGFEVEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYD
                     LIINIANVNPGTVQRIVWPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYIN
                     TYDGKQETLEILVDKLMGLSEFKGISPIDAYCGLKDTFI"
     gene            630..2438
                     /gene="uidA"
                     /locus_tag="ACG81A_002620"
     CDS             630..2438
                     /gene="uidA"
                     /locus_tag="ACG81A_002620"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFS5015932.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
BASE COUNT          915 a          307 c          490 g          726 t
ORIGIN      
        1 ctgatcaagc aattacacta gttaaagata aacaagatat ttggcctatt gtaccagaaa
       61 aatataaaag gattttactg gtagatgtac atggggtaaa aggtgggttt ggtgaagtag
      121 ttgctggaaa tagacctcag gccattgatt tattgaaaca gattttgtca gcaaaaggat
      181 ttgaagttga aaaatacatt tcacctgaag aagaattgaa aggattatcg caagaagaaa
      241 aaattaaaaa tatagctgga atatatgctg ctaagagacc aatcaaagat ttaactgaaa
      301 aatatgactt gattattaat atagctaatg ttaacccagg tacagttcaa agaattgttt
      361 ggccagcaag taaaggtact ccggatattc cattttatat tcatgaaatt ccaacaattt
      421 ttgtatctgt acagtgtccg tttcatttag ctgatgtgcc tcaagtaaaa acttatatca
      481 atacttatga tggaaaacaa gaaactttag aaatattagt ggacaaacta atgggccttt
      541 ctgaatttaa gggaataagt ccaatagatg cttattgtgg gttaaaggat acatttattt
      601 aatatttaaa ttatatagga gtgatttaca tgttatatcc cattatgaca gagacgaggc
      661 aagtaattga tttaagcggt ctttggagat ttaagttaga tacaaatgat gacttacaag
      721 aagaacttgc tttagaacca atggttgaaa aagagagtta tccaatttca gttccttctt
      781 cttataatga tttatttgaa acagagaaca ttagaaatca tgttggatgg gtgtggtatg
      841 aaaaagaagt gattcttcct aaaaaattat tagaagaaag attagttctt cgttttggat
      901 ctgcaacaca tgaagcaaaa gtatttttga atggaaaatt gattaatcaa cacaaaggag
      961 ggtttacgcc atttgaatca gaaattaatc agtttataaa agatggaagt aacagaatta
     1021 cagtggctgt aaacaatgtt attgacgata gcactttacc tgtagggatt ttgaaagaaa
     1081 cgatacagga agatggagac ataaaaaaag aaaatttggt taattttgac tttttcaatt
     1141 atgcaggcat taatagaccc gttaaattgt atacaactcc gaaagactat ataaaggatg
     1201 tcactattaa tacaaaaata attgattcag atgcaaatgt agaatttaat atcgatacag
     1261 aatggaccgg agaaattcta atagcaatct atgacgaaga agctcaactt atagcagaag
     1321 ctaaggggaa agaaggagtg attcaactta agaacccaac actttgggag cctctaaatg
     1381 cttacttata tagtatgaaa gttagtttaa ttaacgaaaa taaagtgatt gatacgtaca
     1441 cagaagaatt tggcattcgt agtattcagg tttcgaatgg gaagtttttg attaataata
     1501 aaccttttta ctttaaggga tttgggaagc atgaggacac gtatattaat ggtcgtggtt
     1561 tgaatgaagc agtaaatatc aaagatttta atttaatgaa atggatcggt gctaattcat
     1621 ttaggacttc acattatcct tattcagaag aaattatgcg cttagcagat cgagaaggta
     1681 ttgtagttat tgatgaagta cctgcagtag gattacattt gaatttcatg gctactttat
     1741 tagatgacaa cgtagaaaaa catgatactt ggaaagaaat acggacagaa aatgctcaca
     1801 aaatggcgat taaggaattg atagaacgcg acaagaacca tccgagtgtc gtaatgtggt
     1861 ctattgcaaa tgaaccagat tcggattctg aaggcgctaa agaatatttt gaacctttag
     1921 ttgaattgac taaagaactt gaccctcaga agagacctgt aacaatcgtt acttacttaa
     1981 agtcaacacc agatatttgt aaagtaggag atattgtcga tgttttatgt ttaaatcgct
     2041 attatggttg gtatgtttca ggtggagacc taaaacatgc aaaatatctg ttgaaaaaag
     2101 agctagatgg atggatggaa agatgtcccg atacgccaat aatgtttaca gaatatggag
     2161 cagatacagt agctggactt catgatacag taccaacaat gtttacagaa gagtatcaag
     2221 tgggttatta cgaagcaaat cacgaagtaa tagataaatg tgacaatttt gttggtgaac
     2281 aagtgtggaa ttttgcagat tttgaaacaa gtcaagggat tttaagagtg caaggaaata
     2341 agaaaggaat ctttactaga gatagaaagc ctaaaatgat tgctcactat ctaaagaaac
     2401 gatggtcatc aataccaaat ttcagttata aaaaataa
//
LOCUS       DAZULY010000276         2425 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000276 DAZULY010000000
VERSION     DAZULY010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.281"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..2274
                     /locus_tag="ACG81A_002621"
     CDS             121..2274
                     /locus_tag="ACG81A_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFS5015933.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          828 a          425 c          537 g          635 t
ORIGIN      
        1 aaaagttgat tttatcttga aattaggtaa agaatttctt gctccgccca ttttttcttt
       61 tttattaaga agtttttggg tacaatgaag catgaatgta aataggcgga aggataggaa
      121 atgatgaaag attttatgaa aaaaatatta aatacttctt ggttagaaaa aataagaaaa
      181 ggtgcacaag aacctaagaa caaaacgttt cgccggtccc atgttccttt tcgcttaaat
      241 tttctttttt tcattatatt tacgttattt gttgcgttga ttgctagatt gggttatttg
      301 cagatcgtga acggcgaaga gatggaggcg cgagtgaagt ctacttctac gattacggtc
      361 caagaaagta ctcctcgcgg aatgatctac gactccacag gaaaagcttt agttacgaat
      421 aaagcgaatc aggccatcac atatacgaga ggaacacaag ttacggctaa agatttgctt
      481 gaaatcgcaa cgaagctgaa ctcgctgatc gatgttccgg tagatgaaaa tctgacagat
      541 agagataaaa aagatttttg gctggctgat cccaaacatt tgaaagaagc cacaaaaaga
      601 ctttcggcaa aagaaaagca attagggaac agcgaacaat atagtgcgat ggtcaataaa
      661 gtcacagctg atgagattaa tttaaacgag gagcaattaa aaattgctac catctttaaa
      721 cggatgaata gtgcatatga attaaacact gtctatatca aaaactctgg tgttactgat
      781 caagagttag ctgttgtggc agaacgcgct tctgaacttc caggagtttc gactggcaca
      841 gactggacac gagaatataa tgcagcaagt tcactgaaaa gtattcttgg cagtgtgacg
      901 actgaaaagc aaggacttcc agcagatgaa gcagagaaat acctagcaaa aggttattct
      961 cggaatgacc gtgtaggaca aagttatttg gaaaaacaat atgaagatgt cctgcaagga
     1021 accaaaaccc aatatgaagt ttcccttgat aatgaaggaa atgtttcgaa tcaaaaagaa
     1081 atcttctctg gggaaaaagg ttctaattta atgttatcta tgaatgcaga attccaatca
     1141 aaagtagaag aaattttgaa aaggaattat caaacattga tcaacaacgg caaagcacag
     1201 tattcgcctg gtgcttatgc agtggctatg aatcctcaga caggagaagt gctagcaatg
     1261 actggtttca gtcatgaaca aggttccaaa gaaatcaccg agaatgcttt aggcacgatt
     1321 actagcgcct ttgatccagg gtctgtagtc aaggctggta ctttgacagc aggctgggca
     1381 agtaacgcga tcagcggaaa tcaagttttg attgatgagc cgattcgtct gcaaggtgct
     1441 tcagaaaaaa gctctgtctt taatcgaagc ggacaagttg ctcttgatgc agtcaaagca
     1501 tcggaattgt cttctaatac gtatatgatc aaagtggcgt taaagatgtt aggactagac
     1561 tatacacctg gaatgggact acctagttta gatgaagaag ctaaagctta tcagcaattg
     1621 agagactctt tcaaagagtt tggtcttggt acaacgactg ggatcgattt gccgaatgaa
     1681 tctcctggga tctcacgttc tgttgattat atgaagaagt tcaatgcgga taatggcaaa
     1741 gaatggtatg ctccaggtaa ctttaccgat ttggcatttg gacagtttga cacatataca
     1801 ccaatccaat tagctcaata tgcttcaaca gtcgctaatg gcgggaaacg agtacagccg
     1861 cacctagtca aagcgatcta tggcaatgac gaaaatggaa acttgggtga agtgaagaaa
     1921 gaaattggca caacagtaga aaatacggtc aatatttctg cagaaaatat ggcgatcctc
     1981 cgtgaaggat tccaccaagt tgtccatggt accgatcctt acacaacagc caaaccatta
     2041 gccagtgcga aaatggacct ttccgcaaaa acagggactg cagaaacggt tgcggaaggt
     2101 catccagata ttgcgacagt caatagtaat atcgtggcat acggaccaac agacaatccg
     2161 gaaattgcca tcagtgttgt cttgccgaac ttattagacg aacaagacca cataaacttg
     2221 acgattgcta aagaaataat ggacgcgtat tatgatatgt tcatggcaaa ataaaaaaga
     2281 ggtcgtgaaa aagccgctta gtttcgagta tgaagaaaaa ttttgtgaaa atagcttctc
     2341 acatttttgc ttaaatttga cttcataccg agaaactggc ttttgaacac cgtttactcg
     2401 gcaaaaaagg ttgtgacaaa aattg
//
LOCUS       DAZULY010000277         2408 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000277 DAZULY010000000
VERSION     DAZULY010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.282"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..464)
                     /locus_tag="ACG81A_002622"
     CDS             complement(243..464)
                     /locus_tag="ACG81A_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015934.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..807)
                     /locus_tag="ACG81A_002623"
     CDS             complement(562..807)
                     /locus_tag="ACG81A_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015935.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1047..1661
                     /locus_tag="ACG81A_002624"
                     /pseudo
     CDS             1047..1661
                     /locus_tag="ACG81A_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2031..>2408)
                     /locus_tag="ACG81A_002625"
     CDS             complement(2031..>2408)
                     /locus_tag="ACG81A_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFS5015936.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          816 a          419 c          332 g          841 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 ataatattta tctttccttt tcaatctaag aaatcgtcac cccattcttt ttcttctatt
      901 ataaactatt taacttttag aattcaaaaa ccgtttgtct atttagagtt taccctaaat
      961 agacaaaaat ataacattga aaaatttaga gttctaacgt atgattaggg tgtattataa
     1021 acgttataga aaagtaggga ataaatttga aaattgctta tattagagtt tcatccatcg
     1081 atcaaaatga acaacgtcaa attgaagaaa tgaaaaagtt tggcgctgaa aaaattttta
     1141 tcgaaaaaca atctggagcg acgattagtc atcggccgat tttccaggaa gctttagatt
     1201 ttgtcagaga gcaagatatt tttattgtag aagccattga tcgacttgga cgcaattatg
     1261 acgagattat cgattcagtg aattatctaa agaagaagaa tgtgcgactt attatcacca
     1321 gtttaccgat catggctgaa gcaattggaa atcctctcct agataaattc atcaaagact
     1381 taatcattta aatctcagcc atgattgctg aacaagaacg tactgaatcg aaacgacgcc
     1441 aggcacaagg aattaaaatc gctaaagcaa acggcgtcta taaaggtcgt cctaagttat
     1501 acagtgcaga caccaaagat cctcaacgtc gccttgtcta taggagtatc gttcaggatc
     1561 ttgagaatgg ggttgctatc tcaaagattg caacagacta caatgtgact aggcaaacaa
     1621 tttaccgaat aaaaaaagaa atcgaccaac taattgtatg atttcttttt tgaatcacgt
     1681 aattattaat tagctttgtc atcaatacag acaatcattt tagattcatc tagctaagtt
     1741 gattacattt atgttaaaaa gaatttagtt aacataaata taattgatgc ataaatcgtc
     1801 cttatttaaa ggcatttaac attgacaact tagtataatg tatcttatgt aaactaataa
     1861 gatttgaatt ttattttata cacactcgat aatagatcaa ttttatggtc ccccgcattt
     1921 acgattgcac aagagaaaca taaagtcgac ctattagcat ttcttttcaa ttcaaacatt
     1981 aaaaaagttt atacctctgc tactaaaaaa tgaaattatc tatttaaaat ttaaaacggt
     2041 aaatctgatt catccaaatt ggaaaatgga tcagcctgta ttggagcttt gtatttctcc
     2101 ataataacag atatcttttt ttcatttttt aaaaaatacc ttttaaattt cttatagtcc
     2161 tctgaaatca aaatatagcg tgatggatac tcatcaaaag aagaatcgta ttcttccttt
     2221 aaaattcccg atttacttaa tttttctagt acatcaaata ctctatcact caagtaactc
     2281 cttaaattgt tatcactttc ccattgaact acttttctat aagttgaatt atcagttgaa
     2341 aagatagaat tttcatccat aaacgcatat attagcagca atctttcatc attgtcaaat
     2401 tctattgg
//
LOCUS       DAZULY010000278         2404 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000278 DAZULY010000000
VERSION     DAZULY010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.283"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            5..1150
                     /locus_tag="ACG81A_002626"
     CDS             5..1150
                     /locus_tag="ACG81A_002626"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFS5015937.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="ACG81A_002627"
     CDS             1321..1779
                     /locus_tag="ACG81A_002627"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFS5015938.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1937..>2404
                     /locus_tag="ACG81A_002628"
     CDS             1937..>2404
                     /locus_tag="ACG81A_002628"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFS5015939.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          800 a          452 c          455 g          697 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaacggg aaaacaaata gctagttttc agtttagcgc tctttttatt atataaaata
     1921 gaaaggagaa aactttatgt ctatttataa ttttgaaact actttagaag acggcactgt
     1981 ctattcattg aaaaaatatg caggcaaacc gataatcatt gttaacactg caacaaaatg
     2041 cggtctagca cctcaattta aacaattaga agaactttac caagcttatc agacacaagg
     2101 tctggttatc ttaggctttc cttccaatca atttaaacaa gagctagatt cagcgactga
     2161 agcagcagaa gcttgtaaaa ccacttatgg cgttaccttc cctatgcatc aattgattga
     2221 cgtaaatgga gaaaatacag atcctctttt tacttatctt aaaaatgaag ctcctggtac
     2281 tgtcactaaa gcaataaaat ggaattttac taaatttttg attgatagaa atggcaaagt
     2341 aatcgaacga tttgcaccac aaacttctcc attgaaaatg accgctgcaa ttgaatccgt
     2401 tctt
//
LOCUS       DAZULY010000279         2396 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000279 DAZULY010000000
VERSION     DAZULY010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.284"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..2069
                     /locus_tag="ACG81A_002629"
     CDS             207..2069
                     /locus_tag="ACG81A_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5015940.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2396
                     /locus_tag="ACG81A_002630"
     CDS             2069..>2396
                     /locus_tag="ACG81A_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFS5015941.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKN"
BASE COUNT          864 a          353 c          466 g          713 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaata
//
LOCUS       DAZULY010000280         2383 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000280 DAZULY010000000
VERSION     DAZULY010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.285"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..>2383
                     /gene="esp"
                     /locus_tag="ACG81A_002631"
     CDS             57..>2383
                     /gene="esp"
                     /locus_tag="ACG81A_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFS5015942.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          864 a          373 c          485 g          661 t
ORIGIN      
        1 agatttatct tttattctta ctaggaaagt aattattaag ggagttgatt tgaataatgg
       61 ttagcaagaa taataagaga gtatttctag aaaaaacaaa gaaacgagta ttgaagtaca
      121 gtatcaaaaa attaagtgtt ggggttgcat ctgttttgat tggtgttggt cttgtcttgg
      181 gaacaactga actagttaaa gcacaagatg aaatttctcc aagcagccca cttgaaacag
      241 cgataagctc cgtacaagta ggtgacaaag tagcttcggg gaatactttt caggagaatc
      301 ctggttatac gaaaaattat aacttttcgg atttacaatt tagtcctcaa gaactaactg
      361 gagacacact aaaaggcaat acaattggat ttgaagttta cggaaaacat aatattgcag
      421 catcaactaa aaactgggaa atccgtcttc aattagatga acgattagct aaatatgtag
      481 aaaaaattca ggtcgatcca aagaaaggaa ttggatcttc tagacggacc tttgtaagaa
      541 ttaatgattc gctgggtaga cctacaaata tttggaaggt caattatatt cgtgcaagtg
      601 atggattatt tgctggggca gaaacaaccg atacgcaaac cgctcctaat ggggtaatta
      661 cgtttgaaaa gagcctagat gaaattttta aagagatagg catagacaat ctcaagaccg
      721 atcgtttaat gtatcgaatc tacttggtaa gtcatcaaga cgatgataaa attgtaccgg
      781 gtattgatag tacgggctac tttttaacag attcagatga tttctataat agcttagatg
      841 tatctgaaaa taatcctgac cagtttaaac atggatcagt aagtgcaaaa tatgagaaac
      901 ctaatactca aacaaaagat ggttcaggat ccacaggtgc taatggagct ataatcttag
      961 atcataagtt aacgaaaaat tataatttct catattcatc ttccgcaaag ggcacgcctt
     1021 ggtatgctaa ttacaaaatt gatgaacgat tagttccata tgtagcgggt atacagatgc
     1081 acatggttca ggctgacaaa gtaacatatg atgtttcgtt tgaatccgga acaaaagtag
     1141 ctgatttagc aatcgagcga cgggaaggtc atgaaaatta tggcataggt tcaataacta
     1201 ataatgattt aactaaactt atcgattttg ctaatgcaag tccacgtcca gtcgttattc
     1261 gatatgtttt acaattgact aagccattag atgaaatctt agaagatatg aaagcaacag
     1321 cacaagttga agaaaataaa ccatttggtg aagatttcat ctttgattct tggttgtcgg
     1381 atacgaataa aaaattaatc cagaacactt atggaacagg ttattattat ttgcaagata
     1441 ttgatggtga tggaaaccct gacgataaag aagagagcgg agacacgaat ccatatatcg
     1501 ggaaacctga attagaagaa gtatatgatg ttgacacaac agttaagggg aaagtattca
     1561 tccacgagtt agcgggaaca ggtcacaaag cccaacttgt tgataaagaa ggtactgtat
     1621 tagcagaaaa aactatcgct ccaaatgaaa aagatggggc tccaatttca gatactgtag
     1681 aatttgaatt tacgggtgta gattcaagta aattaatcgc gaaagatgaa ttaaaaatcc
     1741 aaatcgtttc tccaggtttt gataaaccag aagaaggttc aaccgttatt aaggaatcac
     1801 caaaagcggt tgataaacaa accgtggtag ttggatttaa accagatgct aaagaatcaa
     1861 ttcggaataa taaaaactta cctgaagatg cagagtattc atggaaaaca gagcctgata
     1921 cttctaacgt tactgatagt acgaaaggta ttgtaactgt taagatcgga aatcgaactt
     1981 tcgacgtgga tgtagagttt gctgtaaaag cttctcaagc tatggaaaat gatgcaacat
     2041 acgtacctat aacaacaact ccagaaacga cagttcaaag tggaaaacct acatttgata
     2101 aaccagatgt tcctttagct aaagatgcct tttcaatttc agatgtctat aataagtact
     2161 tcggtaatgc aagtgttgat gcaaacactg gtattgttac attcactcca gctaaaggtg
     2221 taggagaatc ggagccgatt actggaacaa ttcctattaa aattgtttac caagatggtt
     2281 ctgtaggcac gaccgattta gcagtaactg taagtaaaga tatttatgaa aatccaggag
     2341 aaaacattcc tgcaggctac cacaaagtaa ccttcaccgc agg
//
LOCUS       DAZULY010000281         2379 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000281 DAZULY010000000
VERSION     DAZULY010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.286"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..494)
                     /locus_tag="ACG81A_002632"
     CDS             complement(66..494)
                     /locus_tag="ACG81A_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015943.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(522..1160)
                     /locus_tag="ACG81A_002633"
     CDS             complement(522..1160)
                     /locus_tag="ACG81A_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS5015944.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1164..>2379)
                     /locus_tag="ACG81A_002634"
     CDS             complement(1164..>2379)
                     /locus_tag="ACG81A_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFS5015945.1"
                     /translation="YVFLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFS
                     LLLTVANVELATIVFSSSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFAS
                     AADQELYETLNKNGSILVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQIT
                     EKETHPVILIPERYRHTDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPW
                     IEHYPVVEILTLKNSMYLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQL
                     SFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSS
                     GYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILST
                     EKKSQLLLMNGG"
BASE COUNT          759 a          450 c          358 g          812 t
ORIGIN      
        1 ccaaaaataa cttttaacgc tgtttcatta tatgtttttt ctattttata taaacttttg
       61 gatacttaat accaaccggt tggattgtac caacatgaga atgtgtcagt tctatttcct
      121 gtcgcacttg cataggccca tactcctttt gttgcatttg ctgatcgaat agagtttcca
      181 actttcgctt ttgcaccgtg acgattatag acacgactat agtggttaga atgacctgtt
      241 tttctcgtac aagttgtatc actataataa acccatcttc cagagctatg attaattgct
      301 gttctagaaa tttcccggtc tactggtaaa tttgacttaa taatattacc tttagcatca
      361 aaatgaatct ctcctggctt taaagatttg taatagattt caccgtgaac tgaatttgct
      421 tccacttcag atgacatagc gtttcctcca acaaaaccta tccctagtgc acaaataata
      481 actgcttttt tcataaaacg acatcctttc ctttttgatt tttaaagcat gatcttcttg
      541 tttcctattt tggtcagata tggatcatgc gtgactacga taactaacgt accttgggaa
      601 gcttgttgcg ataagatttc aactacttta tccttatttc ttttatccaa tgacccagtc
      661 ggttcatcgg ctaaaatgat tttgctattc ttcaataaga ctctcgcaca ggcgattctt
      721 tgcttctcac caccagataa tgtgtagata ggcgtactta gcttcttgtt tatatttact
      781 tttttcaatg cctcatactt tgctgttcgc ttatctgaaa catttaaatt cgaaaacttc
      841 aatccaatat ctaaattaaa atcgattgtt tcatattcaa tcaacccaaa atcttgaaaa
      901 agaaaagata tggtatttct ttttatcaag cgcttctctc tcccagttaa ttgtttcgtc
      961 tcttgatcga acaaatagaa ttgtcctgaa ctgaactgat cattcaatcc taatatattc
     1021 aataacgtac ttttacctgc tccactttct ccaacaacag tcacaagatc cccagcatcc
     1081 gcagtgaaac gatccaaatg aagaatcgtt ttgtttcgaa tgattttttt cgcacctttt
     1141 atcactatca acggctccaa ttgttaacct ccattcatca gcaataactg acttttcttt
     1201 tctgtagaca aaataaaaac aactgaaaga ataaaatcaa tgaagctaaa gaaaaaaata
     1261 ttaaaaagat atttaggctc tctttctata agaaaaagga agattacact tgtcgttccc
     1321 catttgatga ggttcatcat gaagaaatct ttatacgttt caaagagcga ataaccactt
     1381 gaacgaaaga tagcgatttt ccgacaattg tagacaaaat atatgcataa catgacatag
     1441 ctcaacaaga agaaagaaaa gaaagtgagt aaaaatatct gtatcaagta atgaaatgat
     1501 ctgcttaaca ctttgacagc tatctcttca gtctgagtat acggaaatga taactgcaag
     1561 ttatcacgta actgactttc ttcaatcagc tcaaataacc tttctttcga caaagagcct
     1621 atcttgatct ttaacggtgg atagatttct ccgctgagaa tatttctttc caaatacata
     1681 ctgtttttca atgtcagtat ttcaacaacc ggataatgtt cgatccaggg gatagaagga
     1741 ataaatgtat aaatgggttg attgtttttt ataaatagaa atttcggttc tttttcactg
     1801 atttcttgat aatattctgt tatttgcgcc aggtcatttt ttaaatctgt atgtctatat
     1861 cgttcaggga tcaatataac agggtgcgtt tctttttcgg ttatttggat caaccttcca
     1921 gagacatcaa tcaaaggaaa tttcttaaga taattcagat ttatttttat ccctctgcca
     1981 aactgttcat tttcttcatt caagtaatct gatacattga ctaatatact tccgttttta
     2041 ttcaaagttt catataattc ctgatctgct gctgatgcaa aacgattgga ataaataaaa
     2101 tccacgtggt ttttcccgat ggttactgga taaaatacat gatagtcctc accagcgatt
     2161 tcttttggtg gttcatggga aacagctaac tgtgaagaag aaaaaacgat agttgctaat
     2221 tcgacattgg caacggtcaa aagaagagaa aacaacaatc caaatgtaaa aagaatgtat
     2281 tggatatagt tgaacgcttc tcttaacgaa gcgatttttt caactaataa aagagaaatc
     2341 aaagagatac catatattcc gctgaacaag aaaacgtac
//
LOCUS       DAZULY010000282         2368 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000282 DAZULY010000000
VERSION     DAZULY010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.287"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..1116
                     /locus_tag="ACG81A_002635"
     CDS             70..1116
                     /locus_tag="ACG81A_002635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS5015946.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            1133..2356
                     /locus_tag="ACG81A_002636"
     CDS             1133..2356
                     /locus_tag="ACG81A_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS5015947.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
BASE COUNT          813 a          399 c          490 g          666 t
ORIGIN      
        1 aaatagggct taagtttgag tgacagtttg taggctatgt tatactatat aagtgaaggg
       61 ggacgtaagg tgaaggtatt attatatttt gaaagtgaaa agatgcttgc aaaatctgga
      121 atcgggcgtg cgcttgatca tcaaaaacgt gctctgacgg aagtcggtat ctcttataca
      181 ctagatgaaa aagaagatta tgacatcctg cacatcaata catacggaat caatagtcat
      241 aatatggtca ataaagccag aagagaagga aaaaaagttg tctatcatgc gcattctaca
      301 gaggaagatt tccgaaattc ctttatcgga tcgaaccagc tttctcctat cgtaaaaaaa
      361 tatctggtag gattgtacca aaaagcggac tatctgatca cgccaacacc ttattcaaaa
      421 cagttgttgg aaagctatgg tatccgtgtg ccgattcaag ctatttccaa tgggatcgac
      481 ttagagaaat atcaccctga tccaatgaaa gaacaaaagt tcagagaata tttcaaactg
      541 tctccggatc aaaaagtaat catttgtgtc ggtttgtttt tcgaacgtaa aggaatcatt
      601 gatttcgtag aaattgctaa gaagatgccc gaatacacat ttatttggtt tggtcatgtt
      661 cccatgtact cgattcctcg gaacatccgg aaaattgtaa aagaagatca tcctgaaaat
      721 gtcctgtttc ctggttacat tcgtggagag atcatagaag gtgcgtattc aggtgcagat
      781 ctattctttt tcccttctta tgaagagaca gaagggatcg ttgttttaga agctttagca
      841 agcaaacaaa acgtacttgt tcgagatatt ccagtgtata atggctggtt agaagatagc
      901 aagaactgct atatggggaa gacaaacgaa gaatttatcc gactgatcca acagattacc
      961 aatcaagaac taccgagtac gacacaagcc ggttatcaaa cagcaaaaga gcgaagtatc
     1021 aataaaatcg gtgaagaact taaaaatgta tacgaaagtg tactgaatga aaaagtctct
     1081 agcaaattaa aaaaagtaaa tcaggtcaag gactagtttg gaggcgaaaa acatgaaaat
     1141 cggctttttt accgatacct actttccgca agtcagcgga gttgctacat cgatcaaaac
     1201 attaaaacaa gaattagaaa gacatgggca tgaagtctat atttttacaa cgaccgatcc
     1261 taatgcggat aagttggaaa aagatgtgat tcgaatgcca agtgtaccat ttatttcttt
     1321 caaggatcga agagttgttg ttagaggcat gtggtatgct tatttggttg ccaaagaact
     1381 agaactagat cttattcata cccatacaga attcggagca gggatacttg ggaagatggt
     1441 agctaagaag ttgaaaatac ctctgatcca tacttaccat acgatgtatg aagactacct
     1501 tcattatatc ggtaaaggaa aagtgatcag gcaatcccat gtaaaatact tgtctagatt
     1561 atttgcaaat catacgacag gtgtagtttg tccaagtgaa cgtgttattg atacattacg
     1621 cagctatggt gtaatagcac cgcttcgcgt gattcctact ggtatcgaca ttgaaaaatt
     1681 caaacgttct gatatcactc agcaggatat cagcaaatta agggagtcac taggtcttca
     1741 ggaaaaccat ttgatgattc tttctttaag caggatttct tatgagaaaa atatccaagc
     1801 cttgattaat gggtttcctc aggtcatctc cgcttatcct aacgccagat tagttatcgt
     1861 aggaaaagga ccttatgtag aagagttaga ggaattgatc tcagaactgc agttagaaga
     1921 atttgtgcag ttcacaggag aagtggacaa caatgatgta gcactttatt ataaagcggc
     1981 agattatttt gtcagtgctt ctacttcaga aacacaaggg cttacttata cagaagcgat
     2041 ggcagccgga acaccttgtg tcgtggaagg aaatgcttat ttgaaccgat tgttcgatca
     2101 tccttcatta gggaaaactt ttgaaaccga cgaagacttt gcaccagcac ttattgatta
     2161 tatttccagt gaagttccta aagatccaaa agttttagaa gctaaacttt atgatatttc
     2221 atctacacat ttcggcgaag ctatgatcga attttatcaa gacatggttg tttatcatga
     2281 agaagagatg cgaagaaaag aagaagaaga ctcgattgaa cgaatcaaaa taaaattaaa
     2341 ttcatttaaa agataaaatt caaacatt
//
LOCUS       DAZULY010000283         2331 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000283 DAZULY010000000
VERSION     DAZULY010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.288"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..848
                     /locus_tag="ACG81A_002637"
     CDS             <1..848
                     /locus_tag="ACG81A_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015948.1"
                     /translation="SSQEDKKINCKGLLSLADSEIPTVKISGANYEEHIRRLIEYYLL
                     NDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKWYVKEIKGKKI
                     YTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDIENPLVLSTWY
                     LDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGNAYSHEIKVNV
                     VLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFGNVRSRFMDYF
                     EDNGG"
     gene            852..1676
                     /locus_tag="ACG81A_002638"
     CDS             852..1676
                     /locus_tag="ACG81A_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015949.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            1694..>2331
                     /locus_tag="ACG81A_002639"
     CDS             1694..>2331
                     /locus_tag="ACG81A_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFS5015950.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT          867 a          332 c          459 g          673 t
ORIGIN      
        1 ttagctcgca agaagacaaa aaaataaatt gcaaaggatt actcagtttg gccgatagcg
       61 agataccgac tgtaaaaata tcaggtgcta attatgaaga gcatattcga cgactgattg
      121 aatactactt attgaacgat ccaacaaaac aactaaaaaa tattttggag gtaaaaacag
      181 aaagcgttac ttctcattct tatcaagctg ttgacacaaa taagagacga ttgaatgcat
      241 atataataaa tgggttcaaa aaatataatg ttaaatggta tgtcaaagag attaaaggta
      301 aaaaaatata tactggtatt cgagcagttg atgattcaat ttatattaaa gataattcat
      361 ctgagtttaa tgactgggat gtttttgtgc aatctcctgg accagggaac gagaacaaat
      421 tattgatcgt agataaagca atgaaagata tagaaaatcc cttggtattg tctacgtggt
      481 atttagatga cgaaaataat cttacgcaag attcaacgaa taagaacatt gcattgccta
      541 ccgttaattt agtaaatata tatgatcaaa ctcaagaaga tagaccttct tatgaagagg
      601 tagctaaatc ggagcttaaa ggcaacgcct attcacatga aattaaagtt aatgttgtgc
      661 taaatgctag aaatatcaat gtagaaaata tcgaaacagg aatgttagcg actattgttt
      721 acaaagagca gacttacaaa tctgttctaa ctgcctggcg aatatccagc agtaaaaaga
      781 atatagaatt gacatttggt aatgtccgaa gtcgttttat ggattatttt gaagataatg
      841 ggggataaaa aatggttagc aatgtggatg gatatcaatt tgaaaacgtg aaagtaagcg
      901 cagaaaatga tgctagactt tatcacgttt tatataatcg gaaaaatcag gttattgatg
      961 gttacgatca gtctatgaat ttatcttcaa gcggattaac agtaaaagtt gctgcaggag
     1021 cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga agaaagtata acagttccag
     1081 caaactcaag tggttatata gcattaacag tggatttgac acaagaagtt atacctggat
     1141 ctattcttcc agaatcggaa gaatatgaat ggactaataa tcaagtcaag ctagaattta
     1201 taacaaaagt tataaaaggt aatttaaaca atggtgataa ggtctataat ttaccactat
     1261 gctcagttaa ttctactgga tcaactgttt caatctcaaa gatatcggat agttacgagc
     1321 tgactctctc taaaggagaa attttgtgga gggggactgc gttaatgcat gatactcaaa
     1381 ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg ttttttgtta gtatggctcc
     1441 catacgaaaa cgggcaagca attgaggata gatatgtaac tacgcctttt tataaggagc
     1501 gtataacttt tactaatgtt ttaggagaaa ttgtttcagg atttgatgac tatcacaaaa
     1561 agtggtttag taaacgaata aactataatt cgaatactaa tatatttaca ggtgcagcaa
     1621 gcaatgcaag tggagataac gcaaatatgg tgcttaggta tatagtttct ttttagttag
     1681 gaggtatgga agagtggcaa atttagaaat taaattatct gcaaataaga gacagcctta
     1741 tctacgtcac cgtgttgttg gtagagttgg tgatgggggg ctcacaacaa tcaatgtaca
     1801 acttcttgag gaagatgaaa ttacaccttt tgtaattaat ctaaacggta ctttgaaatt
     1861 tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa ccagaaataa tcgattcgac
     1921 taatgggtta attagttaca cgttcactaa gtcaaatttc agcacgagtc atcaattcaa
     1981 acaagcatat tttgaatatg tagatcctaa cggcaaaaaa gtaacttttc agaacttcat
     2041 catagacgtg ttagaacgag tagatattaa ttcggagcaa gcgaaatact atatttcttc
     2101 attggaaaaa ttacagagtg aaatgcaaac cactttcaat cagttcatta gtgataaaca
     2161 ggtccaatac gatcaaatct actcgaaata taacgaatta gtaagattga taaatgaatc
     2221 agataagcaa gtaaatgatc gtattgataa ggcaaataat cgtattgatc aaaccaatca
     2281 gcaaatcggc gatctcggca agctgaaaaa gatgtacagt aacagcattg a
//
LOCUS       DAZULY010000284         2330 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000284 DAZULY010000000
VERSION     DAZULY010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.289"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(94..165)
                     /locus_tag="ACG81A_002640"
     tRNA            complement(94..165)
                     /locus_tag="ACG81A_002640"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(130..132),aa:Glu,seq:ttc)
     gene            complement(177..250)
                     /locus_tag="ACG81A_002641"
     tRNA            complement(177..250)
                     /locus_tag="ACG81A_002641"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(214..216),aa:Ile,seq:gat)
     gene            complement(263..333)
                     /locus_tag="ACG81A_002642"
     tRNA            complement(263..333)
                     /locus_tag="ACG81A_002642"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(299..301),aa:Gly,seq:tcc)
     gene            complement(338..410)
                     /locus_tag="ACG81A_002643"
     tRNA            complement(338..410)
                     /locus_tag="ACG81A_002643"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(375..377),aa:Phe,seq:gaa)
     gene            complement(414..486)
                     /locus_tag="ACG81A_002644"
     tRNA            complement(414..486)
                     /locus_tag="ACG81A_002644"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Asp,seq:gtc)
     gene            complement(505..578)
                     /locus_tag="ACG81A_002645"
     tRNA            complement(505..578)
                     /locus_tag="ACG81A_002645"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Met,seq:cat)
     gene            complement(591..680)
                     /locus_tag="ACG81A_002646"
     tRNA            complement(591..680)
                     /locus_tag="ACG81A_002646"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(642..644),aa:Ser,seq:tga)
     gene            complement(703..774)
                     /locus_tag="ACG81A_002647"
     tRNA            complement(703..774)
                     /locus_tag="ACG81A_002647"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(739..741),aa:Glu,seq:ttc)
     gene            complement(796..883)
                     /locus_tag="ACG81A_002648"
     tRNA            complement(796..883)
                     /locus_tag="ACG81A_002648"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(847..849),aa:Ser,seq:gct)
     gene            complement(908..981)
                     /locus_tag="ACG81A_002649"
     tRNA            complement(908..981)
                     /locus_tag="ACG81A_002649"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Ile,seq:gat)
     gene            complement(995..1065)
                     /locus_tag="ACG81A_002650"
     tRNA            complement(995..1065)
                     /locus_tag="ACG81A_002650"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1031..1033),aa:Gly,seq:tcc)
     gene            complement(1067..1142)
                     /locus_tag="ACG81A_002651"
     tRNA            complement(1067..1142)
                     /locus_tag="ACG81A_002651"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1107..1109),aa:Asp,seq:gtc)
     gene            complement(1161..1234)
                     /locus_tag="ACG81A_002652"
     tRNA            complement(1161..1234)
                     /locus_tag="ACG81A_002652"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1198..1200),aa:Met,seq:cat)
     gene            complement(1247..1336)
                     /locus_tag="ACG81A_002653"
     tRNA            complement(1247..1336)
                     /locus_tag="ACG81A_002653"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1298..1300),aa:Ser,seq:tga)
     gene            complement(1360..1433)
                     /locus_tag="ACG81A_002654"
     tRNA            complement(1360..1433)
                     /locus_tag="ACG81A_002654"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1397..1399),aa:Ile,seq:cat)
     gene            complement(1447..1520)
                     /locus_tag="ACG81A_002655"
     tRNA            complement(1447..1520)
                     /locus_tag="ACG81A_002655"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1484..1486),aa:Met,seq:cat)
     gene            complement(1548..1620)
                     /locus_tag="ACG81A_002656"
     tRNA            complement(1548..1620)
                     /locus_tag="ACG81A_002656"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1585..1587),aa:Ala,seq:tgc)
     gene            complement(1640..1713)
                     /locus_tag="ACG81A_002657"
     tRNA            complement(1640..1713)
                     /locus_tag="ACG81A_002657"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1677..1679),aa:Pro,seq:tgg)
     gene            complement(1723..1796)
                     /locus_tag="ACG81A_002658"
     tRNA            complement(1723..1796)
                     /locus_tag="ACG81A_002658"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1760..1762),aa:Arg,seq:acg)
     gene            complement(1807..1892)
                     /locus_tag="ACG81A_002659"
     tRNA            complement(1807..1892)
                     /locus_tag="ACG81A_002659"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1856..1858),aa:Leu,seq:taa)
     gene            complement(1909..1980)
                     /locus_tag="ACG81A_002660"
     tRNA            complement(1909..1980)
                     /locus_tag="ACG81A_002660"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1946..1948),aa:Gly,seq:gcc)
     gene            complement(1992..2064)
                     /locus_tag="ACG81A_002661"
     tRNA            complement(1992..2064)
                     /locus_tag="ACG81A_002661"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2029..2031),aa:Thr,seq:tgt)
     gene            complement(2082..2163)
                     /locus_tag="ACG81A_002662"
     tRNA            complement(2082..2163)
                     /locus_tag="ACG81A_002662"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2127..2129),aa:Leu,seq:tag)
     gene            complement(2171..2243)
                     /locus_tag="ACG81A_002663"
     tRNA            complement(2171..2243)
                     /locus_tag="ACG81A_002663"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2208..2210),aa:Lys,seq:ttt)
     gene            complement(2247..2319)
                     /locus_tag="ACG81A_002664"
     tRNA            complement(2247..2319)
                     /locus_tag="ACG81A_002664"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2284..2286),aa:Val,seq:tac)
BASE COUNT          636 a          642 c          543 g          509 t
ORIGIN      
        1 cttagcacaa tcatccataa agataactct gctaagcttt ttaaaatcat tctttcagat
       61 aaaaagaaaa agatactcaa aaagtatctt ccatgacccg tacgggattc gaacccgtgt
      121 taccgccgtg aaagggcggt gtcttaaccg cttgaccaac gggccaataa aataaatggg
      181 cctaaatgga ctcgaaccat cgacctcacg cttatcaggc gtgcgctcta accagctgag
      241 ctataggccc ataaaaacta aaagcgggtg acgagaatcg aactcgcgac aacagcttgg
      301 aaggctgtgg ttttaccact aaactacacc cgcataatgg cgcgggacag aatcgaactg
      361 ccgacacatg gagcttcaat ccattgctct accaactgag ctaccgagcc aaacggttcc
      421 gacgggaatc gaacccgcga tctcctgcgt gacaggcagg catgttaacc cctacaccac
      481 ggaaccaatg tttttagttt ttaattgcgg gagcaggatt tgaacctacg accttcgggt
      541 tatgagcccg acgagctacc agactgctcc atcccgcgat aatatgaaaa aggaggataa
      601 gggattcgaa cccttgcacg gttttacccg cctgacggtt ttcaagaccg ttcccttcag
      661 ccggacttgg gtaatcctcc atgtaaaact atattaaatt aatgacccgt acgggattcg
      721 aacccgtgtt accgccgtga aagggcggtg tcttaaccgc ttgaccaacg ggccaggtaa
      781 aacaaaatat tgaaacggag aaggagggat ttgaaccctc gcgccggttt cccgacctac
      841 acccttagca ggggcgcctc ttcagccact tgagtacttc cccaaaacca ataagatttt
      901 gtactaatgg gcctaaatgg actcgaacca tcgacctcac gcttatcagg cgtgcgctct
      961 aaccagctga gctataggcc cataaaaaac taaaagcggg tgacgagaat cgaactcgcg
     1021 acaacagctt ggaaggctgt ggttttacca ctaaactaca cccgcatggc ggttccgacg
     1081 ggaatcgaac ccgcgatctc ctgcgtgaca ggcaggcatg ttaaccccta caccacggaa
     1141 ccagtgtttt tagtttttaa ttgcgggagc aggatttgaa cctacgacct tcgggttatg
     1201 agcccgacga gctaccagac tgctccatcc cgcgataata tgaaaaagga ggataaggga
     1261 ttcgaaccct tgcacggttt tacccgcctg acggttttca agaccgttcc cttcagccgg
     1321 acttgggtaa tcctccatat gaaacttaca acgccacaat ggaccttgta ggactcgaac
     1381 ctacgaccgg acggttatga gccgtctgct ctaaccaact gagctaaagg tccaggctct
     1441 taaaaatcgc ggcggagggg atcgaacccc cgacctcccg ggtatgaacc ggacgctcta
     1501 gccagctgag ctacaccgcg aaaaagatat tcattattta aatgaaatgg agcctagcgg
     1561 gatcgaaccg ctgacctcct gcgtgcaaag caggcgctct cccagctgag ctaaggcccc
     1621 gtgtttaaat ttataattat cgggaagaca ggattcgaac ctgcgacccc ttggtcccaa
     1681 accaagtgct ctaccaagct gagctacttc ccgtttaatt aacgcgccca agaggagtcg
     1741 aacccctaac cttttgatcc gtagtcaaac actctatcca gttgagctat gggcgcatat
     1801 ttgaaatgcc gaggaccgga atcgaaccgg tacggtgatc actcaccgca ggattttaag
     1861 tcctgtgcgt ctgccagttc cgccaccccg gcgcgattgc tttggcaaag cggaaaacgg
     1921 ggttcgaacc cgcgaccccc accttggcaa ggtggtgctc taccactgag ctatttccgc
     1981 gtgtttaaag atgccggcta aaggacttga accctcgacc ctctgattac aaatcagatg
     2041 ctctaccaac tgagctaagc cggcatgtaa aacttaaatt atgcgggtga agggacttga
     2101 acccccacgc cgtaaggcgc tagatcctaa atctagtgcg tctgccaatt ccgccacacc
     2161 cgcaaaaata tgagccgtac agggctcgaa cctgtgaccc tctgattaaa agtcagatgc
     2221 tctaccaact gagctaacgg ctcatatgga ggttaacggg atcgaaccgc tgaccctctg
     2281 cttgtaaggc agatgctctc ccagctgagc taaacctcca tggataactt
//
LOCUS       DAZULY010000285         2321 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000285 DAZULY010000000
VERSION     DAZULY010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.290"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            36..923
                     /locus_tag="ACG81A_002665"
     CDS             36..923
                     /locus_tag="ACG81A_002665"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS5015951.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            1009..1239
                     /locus_tag="ACG81A_002666"
     CDS             1009..1239
                     /locus_tag="ACG81A_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFS5015952.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1296..2261)
                     /locus_tag="ACG81A_002667"
     CDS             complement(1296..2261)
                     /locus_tag="ACG81A_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFS5015953.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT          773 a          383 c          467 g          698 t
ORIGIN      
        1 aagcaaggat ttttgatttt taggaggtcg aaaaaatgaa acttggtttt atcggaacag
       61 gcgtcatggg aagtgctgtc gcacgccatc tactagaagc aggacacgaa gtagctgtct
      121 ataatcggac aaaagcaaaa gctgatcctt tagtaacaga aggagctatc tgggcagata
      181 caccaaaagc ggtagcagaa caaagtaata ttctctttac aatggtaggt taccccaaag
      241 atgtagaaga aatctactat ggacagtcgg ggattttttc tgcagatatc tcaggacata
      301 ttttagttga tttgactaca agtacaccaa gcctggcaga aaaaattgca aaaacagcaa
      361 aagaaaaagg cgcagatgcg ttagatgcac cggtatccgg tggtgactta ggagcaaaga
      421 atggtacact gacgatcatg gtcggtggag aagaagccgt ttatgatcaa gtactgcctt
      481 tattcaaaga atttggtacg acatttacat tgcatggttc tgctgggaaa ggtcagcata
      541 caaaaatggc taatcagatc atgattgcag gtacgatgac aggaatgacg gaaatgcttg
      601 tctatgcaca aaaaaacgga cttgatttaa agaaagtgat tgagacgctt tcaggtggaa
      661 gtgcagctaa ctggtcttta agcaattatt ctcctcgtat tttaaaagaa gattacacac
      721 ctggtttttt tgtcaaacat tttatcaaag atttaaagat tgcattagaa gaagcagaaa
      781 aaatggatct tgtattgcca gcaactaccc aagcattgaa actatatgaa gagttggcag
      841 ataaaggttt tgagaatgac ggaacccaag ctttgatcaa actttggtgg cctgaaggaa
      901 aaataccaga aaaaaaatca taaaaaacga gtttttttag agaaaatcaa tgcaagtagg
      961 ctatgctttt gatacaatat aacagaaata agaaaaggag gacctccgat gacacattca
     1021 caattagtcg caatcataaa acgactggag gctatggtcg agtcagcaga taatgaactg
     1081 caagttcgcc gttttgaaaa agaaggcgta gagaagtgta cagtaactta tgataaagcg
     1141 accgatacat ttgaattaac tgaaacagat actcatcagc aatacgaatt tgataatatc
     1201 gatatcgtag cgatggaaat ctatgactta atccaataaa ttttaggata attgacattt
     1261 gaagttgaag atgcctaaaa gaagccgaga agcaactaat ttagttgctt ttcagcttct
     1321 tttttgttta acagttcaat ttcaaactgt tctttttcta attttttgcg gtaactgatc
     1381 aagatttgta caggatcgat ttcttctttt gacaattgac gtttcaaaga taaatgagct
     1441 tctttctttt cataatagaa gtcaggagaa acgtcaccag aggtaatcat tgtttctgca
     1501 agttgaccat aacaaacgag gtagtgcttt ttctgactct catctgctgc taatggcatg
     1561 ctgaaggtga gcgatggtgt tcttaaaaac tgcttttgtt cctggatccg tctaataaaa
     1621 taagtaacta attcgatatc tttttgaagc ataccggctt tttcgaaatt caactgatag
     1681 atgaaatgtt gttgtctttt tttcaagtaa gtcagtagct ttttatttcg gccttgaaac
     1741 agcccttgga gttccttttt tttctgttca aaagtcattt cctgtaggtc aggaagctgg
     1801 actcgcaaag ctaacaaact aatttcagac aaccaaggca tttgataagt ttcttccatt
     1861 atacgcaaaa tagacggcat ttttttatag agacgaaaag gcccgtatgt tcgagccgta
     1921 ggagtatctg ttaatacaaa tcccttattg gtaatatgta cataattata attactgaaa
     1981 taattcattt gtcggttata aaaaggacgg taatgttgga tcaattgaca ctcgagcaat
     2041 aaagcatcta attccgtgtc tactacgacg aaatcaaaat ctgttatatg atgaatcata
     2101 cgtaatactt tttttgaatg ctgtttattc tgatgaaaat agctgcttac tctatttttg
     2161 agccgttttg ctttaccaac atagatgacc ttaccatctc ggtctttcat aagatagaca
     2221 cccggcagca tggggagagt tttcattttt tcttttaaca taggatctcc ttttacgctt
     2281 tgaataaaaa gactataaca aattttgaaa taatggacaa g
//
LOCUS       DAZULY010000286         2311 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000286 DAZULY010000000
VERSION     DAZULY010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.291"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(121..600)
                     /gene="rlmH"
                     /locus_tag="ACG81A_002668"
     CDS             complement(121..600)
                     /gene="rlmH"
                     /locus_tag="ACG81A_002668"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFS5015954.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            1028..>2311
                     /locus_tag="ACG81A_002669"
     CDS             1028..>2311
                     /locus_tag="ACG81A_002669"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HFS5015955.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSE"
BASE COUNT          729 a          424 c          447 g          711 t
ORIGIN      
        1 tttcttttgc aacttacttg gctaccaaag caaataaaaa agttatttat aaatttctcc
       61 ctaccactac ctattgctat tatttaaaat agggataatt ttttctctta aaaaatatca
      121 ttatttgtgg tatggagccc ccgcattgat ccggaaggca cggtagattt gctcgacaag
      181 gatcagacgc atcaattgat gtggatacgt catttttcca aaagagatct gagcattgct
      241 tcttttcatg acggcttcgc tgagtcctaa agagccgccg ataacaaaaa caaggtggct
      301 ttttccttgg atacctaatt ggtcgatttg tttagcaaaa gattcacttg taggattttt
      361 cccttcaatt gctaaggcat agacgtattc ttgttctttg attttcgcta agatccgttc
      421 cccttctttt tcttttactt gtctcatttg cgcttcgctt aaattctccg gggctttttc
      481 gtcaggaact tcgatcaatt cgatttttgc ataggcattc agccgtttga catactcatt
      541 gataccttgg atcaggtatt tttcttttaa ttttcctact gagataattt tgatattcat
      601 gttttctctc ctttggtctt catgctcact ttgtttatta taatcaattt tttcgtctat
      661 atgtttattt tggctatttt tgttaaaaag atttccccaa cagttatgca caacatccac
      721 aggtttatcc acagaaaaca gtgttatttt accttatttc atcacattca ttaggcaaac
      781 atacgtttta taagaattta tccactttat acacaggctg tggatatgtt ttatccctta
      841 tttttccaat cctatccgcg tagttttaca cagtatctgt ggaaaaagaa aatcaaatat
      901 tttcatctga aaacaatgat ttttcctaaa gtttttcata aaaagttcac agtctcaaag
      961 tagactaaag tcaacaaata agactatact aacagtagat aaatgaatgt aattggagga
     1021 ggaacacatg gatagaaaaa atgtgacacc aaagatgaaa aaaagtaaaa ataatagcct
     1081 ctggcgtaag ttaggtctgg ggcttgttgg agggatcgtt ggaggactac tgacagctgg
     1141 cattttttat gcagcgatgg gcactggcaa caatagttct actgctacaa gcggcaatca
     1201 gaattctgcc ggtgaaacag ttgtggaaaa tgtcaaagtc aatgtagatt ctgatatcac
     1261 caaagcggta gataaagttc aagacgctgt cgtttctgtt atcaacttac aaagtcaaaa
     1321 ccaatcaagc ggttttggcg gactattcgg gcagcaagaa gaaagtagca gctcagatga
     1381 tagtaatttg gaagcttata gtgaaggaag cggcgtgatc tacaaaaaag acgggaatac
     1441 tgcttatgta gtcacgaata atcacgtagt agatggccag caaggtcttg aagtcttgat
     1501 gaaagacggt acgaaggtaa aagctgaatt agttggaaca gatgcttatt cagatttagc
     1561 ggtgttgaag atcaattcag acaaagtcga aacagttgcc tcttttggtg actcaagtgc
     1621 tctgaaggtt ggtgagcctg cgattgcgat tggttcccca ctaggatctg aatatgcaaa
     1681 ttctgtcact tcaggaatca tttcttcctt gaaccgacaa gtgacaagta cgaatgaatc
     1741 gaacgaaaca gtcaatatca atgcgatcca aacagatgca gcaatcaacc ctggaaattc
     1801 tggtggtcct ttagttaata tcgaaggaca agttatcggt atcaactcaa gtaagatcgc
     1861 aagtacttct gaatctactt ctaatgtaag cgtagaagga atgggatttg ctattccaag
     1921 taacgatgta gtgaatatca tcaatcaact ggaaaaagac ggaaaagtga cacgtccagc
     1981 tctaggtatc acaatggtcg acttatctgc cgtttctact cagcaacaag aacaaatctt
     2041 gaagattcct tcatctgtaa ctaacggtgt catcgtgaca aatgtccaaa cagctacacc
     2101 ggctgaaaaa gctggattga aacaatacga cgtcattact aagatcgatg acaaagaagt
     2161 ttcttctggt gtagaacttc aatctgcttt gtataagaaa aaagtgggag attctgtcaa
     2221 aatcacttac tatgaaggct cgaaagaaaa aacaactact gttgaattga cgatcgatca
     2281 atcggcatta aaacaaaatc aaaatagtga a
//
LOCUS       DAZULY010000287         2303 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000287 DAZULY010000000
VERSION     DAZULY010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.292"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            139..1752
                     /locus_tag="ACG81A_002670"
     CDS             139..1752
                     /locus_tag="ACG81A_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS5015956.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          722 a          407 c          447 g          727 t
ORIGIN      
        1 aaaaattttt aggttttatc cacaaattat ttctctatat tttctgtcta ttcaaaataa
       61 acaataattt tccggcacaa ttatttgctg ttttttacca taacatagta cgatcaacgt
      121 agaaaaggag atgtttttat gaaagttgtt gtcattggcg gtgtagctgg cggtccttca
      181 tttgccactc gtttccgtcg attgaatgaa gcgcacgaaa tcattatcta tgaacgcgga
      241 gaaaatattt cttatgcaag ttgtgctttg ccttattatt taggaggtgt gatcactgac
      301 cgtgactcgc tgatcgaacg tacaccagaa atattgaaaa caaaaaacaa catcgatgtc
      361 tttactaaac acgaagtaac agcaatcgat ccttctacta agcgattaac agttaaagac
      421 ctatccacaa atgaagaaat aaaaacagat tacgataagt tgatcatctc ttctggtgct
      481 agaccagatt atccggatat tcccggagtt tttgaagcag aaaacggctt tgtcctccgt
      541 agtgtgacgg atgcggatcg aatcaaatcg ttccttgaag aaaaaaatcc acaacatgtc
      601 gtcattcttg gtgcaggtgt tatgggtctg gaattagctg agaatttcaa gcatcgcggc
      661 ttagacgtga ctttaatcga tcaattgcca caagtcgctt tcccttatga tccagaaatt
      721 gctaatttag tttatgacaa attgctgaaa gaaggattag ccgttcattt agaaacaaga
      781 gttactgaga tccgtgataa aggtcgagaa atagtattat cagatggctc cgtcctttct
      841 gctgatatgc taatttttgc tgttggtgtt tctccgaaga atgaagtggt gaaagcaacc
      901 ggcatacaat tatcagatac aggtcagatc attgttgatg accagttaca aaccaatctg
      961 ccggacatct atgcgattgg cgatattatc gaaacgacta gtgtagtgac tggtcagccg
     1021 atccaaagta tgctttccag tgcggccaat cgtcaaggac atatgctggc agatatttta
     1081 aatggtacgc ctatgcgcta tcgcggatat attggtgcag gtgtcgcaaa aatctttgat
     1141 catacagcaa gttatgcggg aatgacagaa catgaattaa aagcatcagg cataacaaat
     1201 tataaaactg tttttatcac tccctttgac catgcctatt tctatccaga agctacaaga
     1261 ttaaatctaa agctgatttt tgatgcagat agcggtcgta ttttaggtgg acaagcattt
     1321 ggagaaaaag gtgtcgataa acggatggga gaactttctg tagcaatcac cggaaacttg
     1381 acagtctttg atttgcctga tttggagttg ccttactctc caccgtattc tactacccgt
     1441 gacccgttga atatagctgg ttatgtcgca atcaatcaaa tgacgaatat cgtagaaacg
     1501 atcaaagcaa gtgatatacc cgaaaacgat ttgaaagaag cgttcttttt agatatacgt
     1561 gaacctaata aagcaccttc tggcagtatc tcggcaacaa aaaatattcc aatgaatgag
     1621 ttacgagatc gaatcaatga aatcccaaaa gataaaaaag tttacattac tttcagaaga
     1681 ggattgaata cttatacttc tgcccgaatc ttggcaggtt tgggtatcaa agcgattttg
     1741 attgaagaat aaaaaaacga ccgcggtggg tagccgcggt ctaggagtta tttttacttg
     1801 aggagtaaaa atgaaaaagt atatagggtt gttggttggt atgtatatag aatagaagat
     1861 aaatgtgaag aaattgtgca ttatttcttc acatttatta ggttatagtt taagaaagtt
     1921 taaacattgc gcaatttaat tattttagct gtttcttttc ataaaaaaac atgttgtgac
     1981 tctgagacaa atcatagtcc atagtgatgt ctctagagta actacgccta atattacgag
     2041 gcatcatatt aatctatcag cactagtacc taaactagca tccctttcga atattttttc
     2101 ttatataata aagtttgctt tcactcctct aatctcacca aatcgactgg cttgcgttgt
     2161 gtgatactag gaactgattg gtttagaacg acttttctat tgtaatcata tttttcgcca
     2221 tcctcgttgt cttctaatcc ttcaatgaat gatccctaat gtgtgtatta aataatttaa
     2281 tttttttgtt cctatttatt taa
//
LOCUS       DAZULY010000288         2235 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000288 DAZULY010000000
VERSION     DAZULY010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.293"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..2118)
                     /locus_tag="ACG81A_002671"
     CDS             complement(34..2118)
                     /locus_tag="ACG81A_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFS5015957.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT          646 a          509 c          355 g          725 t
ORIGIN      
        1 cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt ttacatgcaa
       61 gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa atactgcatc
      121 tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta tcccaatagc
      181 aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga ttgcttctgc
      241 aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg cagcatcgct
      301 tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg catcgttgat
      361 tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct ctaaccgctg
      421 cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt gcttcccgat
      481 atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca caccagcctg
      541 ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa taacaagata
      601 accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt gacttaaagg
      661 aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt gtttgccatc
      721 aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg gtaaaagcgg
      781 acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact gttctgcact
      841 cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct ggatcgaaaa
      901 gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt gcgcaagcaa
      961 ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc cgataccgcc
     1021 aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca ctaaaaatgt
     1081 caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg gaggtaaaac
     1141 agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga atttagtaat
     1201 gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta ataatttgct
     1261 gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat ttttattgat
     1321 aaatccgctt aatactgttt caccaacata tacagattta gggactgatt ctcctgtaag
     1381 tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg gtacacgttc
     1441 accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca cggtcatcaa
     1501 tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt cgcgaatcga
     1561 ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat aaaatagcat
     1621 cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag caacagacat
     1681 aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct tcttcactac
     1741 atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg gttcattaaa
     1801 atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga acagcgtatt
     1861 tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg ttactttgac
     1921 atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag ccaaatgatc
     1981 ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt ccttgactcc
     2041 attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat ctaaaccttc
     2101 taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca tctcttcata
     2161 actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa aatgaaaaac
     2221 atctgcatcg gaaaa
//
LOCUS       DAZULY010000289         2234 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000289 DAZULY010000000
VERSION     DAZULY010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.294"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..535)
                     /locus_tag="ACG81A_002672"
     CDS             complement(<1..535)
                     /locus_tag="ACG81A_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5015958.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQP"
     gene            complement(535..1491)
                     /locus_tag="ACG81A_002673"
     CDS             complement(535..1491)
                     /locus_tag="ACG81A_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFS5015959.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1491..2147)
                     /locus_tag="ACG81A_002674"
     CDS             complement(1491..2147)
                     /locus_tag="ACG81A_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFS5015960.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          659 a          483 c          424 g          668 t
ORIGIN      
        1 gcggctggtt cttttggttg ataaaccgat aattcagcac attatatgtt tcctgtggca
       61 gtttggaaca aaaagaatca accatatcca gttctttttc tccaccttca tgaccatagt
      121 agacgacaag gatcaatcgt ccgcgaggca caagtcgttt caatatttct tccatagctg
      181 tgcgtgtagt ctctggcata gtgatgattg ctttatcact ttttgggaga taccctagat
      241 tgaaaatccc tgctttgaga ttttgttgtt cagctatcac ttctcctaac gtttcgtggc
      301 cttgtagaaa caatgagaca cgttcttcta actgacgctg ttctagcctt tcacgagtag
      361 catcgattgc ttgttgctgg atatcaaaac tatagacatg accagttttt ccgatgtgtt
      421 ctgctagaaa aaccgtatcg tggccattgc ccatcgttgc atctacgacg tgatcccctg
      481 gttgcaagat ttcttgcagt aatgtatggc tgaaatgcaa agcagtttgc aacatcagac
      541 gctcaccttt ttccgtacat catattttcc ttggaagcta tctctgcgaa gaagttcttc
      601 atcaattgca ttcaagactt cccatttttt caagctccac attggtccaa tcaaggaatc
      661 ccacggcgca tcccctgtca aacgatggat gatgatctct tttggtatca tttccagctg
      721 gtcgcaaatg acattgacat agtcaggacg gctcattaat tgaaggcgac cttcatgata
      781 gtcccgcaac attctcgttt tgcgcatcag atgcatcaaa tggagcttga ttccttggat
      841 atctgaatct aaaatggtgc gtctgacatt ttctttcatc atttctaaac tttcgcctgg
      901 taatccgttg atcaaatgcg tacaaacacg gatattatgt ttgcgtaatt tagccacgcc
      961 atctaaataa gtttgataat catgtgctcg gttgatcagt tttgaagttt cttcaaaagt
     1021 tgtttgcaat ccaagttcta cccataaata catccgctcg ttcagttcag caaggtaatc
     1081 aactacttca tctggcagac aatccggtcg tgtaccgact gataatccta cgacacctgg
     1141 taaattgact acttgctcaa atcgttcttt gattatttca actggcgcat gggtgtttgt
     1201 aaaattttgg aaataaacga tgtattgttg gacatgaggc catttttgat gcatcatgtc
     1261 gatttctttc tggaattgga ttggaagagg gtcttcaggt gcaacgatca tgtctcccga
     1321 accagataca ctgcaaaaag tacaaccgcc atgggcaaca gtcccatctc gattcggaca
     1381 gtcaaatcct ccatcgatcg gcaccttgaa aattttcccg ccaaactcat ttcgtaaagc
     1441 gtaattccaa gaatgatatc ttttatttcc ttctgaataa ggaaaagtca ttaaaagtcc
     1501 ttctttctgt aagagttatc tatttgtttt cttttttctc tatacactgg ataagtcagg
     1561 cacagccaag aaagactgag taagtaaccg ccgatgatat cagtaggaaa atgaacgccc
     1621 agataaatgc ggcttatccc gacacttact ataataaaca tcatcagcaa ttgaagcaat
     1681 gtgcgtaagg ttcttttttg aacaaaagct ggaagcagaa atagtaaaga accgtaaaga
     1741 atcatactgc ctgctgcatg tccactaggg aaactgtaac ttgtttcaac tactaagtgc
     1801 gccaatgaag gacgctctcg catgaaaaat agtttgatca ccgggttgat gattccagca
     1861 attacaataa atccagaaac gaaccaaaat gtttctgccc tttttttctt ataccacatc
     1921 cataaagccg cacaagcaaa taaaatgatg acggtaagag gattaccgaa tttagtcata
     1981 aagagttggt agctgttcag atcaggataa gaagctctga caatgcctgt aaaccactga
     2041 tcgaaaccag aaagccacga cagatggaac tttacgatcc agcccaaaat gaaaaacagg
     2101 acaagaaaga agctaccagc cacctgaaat gatgttttat atttcataca attcctcctt
     2161 tgaagcaatg acttattata gcatttttgg tccagcgaaa aaaacggtaa agagataaaa
     2221 ccaatgtatg attc
//
LOCUS       DAZULY010000290         2205 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000290 DAZULY010000000
VERSION     DAZULY010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.295"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..610
                     /gene="ahpC"
                     /locus_tag="ACG81A_002675"
     CDS             47..610
                     /gene="ahpC"
                     /locus_tag="ACG81A_002675"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFS5015961.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            620..2149
                     /gene="ahpF"
                     /locus_tag="ACG81A_002676"
     CDS             620..2149
                     /gene="ahpF"
                     /locus_tag="ACG81A_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFS5015962.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
BASE COUNT          720 a          372 c          472 g          641 t
ORIGIN      
        1 aaattacaat aattataaat aaataattat tattggaggt tttactatgt cattgattgg
       61 gaaaaaaatc gaagaattta atgcacatgc ttatcatcaa ggagaatttt tagaagtcag
      121 tgaaaaagat cttatgggga actggagtat tctttgcttc tatccagctg attttacttt
      181 tgtttgtcct actgaattag aagacttaca agaacaatac agtacattga aatctttagg
      241 tgttgaagtc ttttcttgct ctaccgatac ccactttact cacaaagcat ggcatgatac
      301 atcagatgct atcggtaaaa tcgaatacgt gatgatcggt gatccgtctc atcagatttc
      361 tcgtattttt gatgttttgg atgaagagca aggattagct cagcgaggaa catttattat
      421 tgatccagac ggaatcgttc aagctatgga aatcaatgca gatggaatcg gtcgtgacgc
      481 tagtgcactg attgataaga ttcgcgctgc tcaatatgta cgtacccacc caggtgaagt
      541 ttgtccggca aaatggaaag aaagtggcga aacattgaaa ccaagttttg atcttgttgg
      601 aaagatttag gtggtggata tgttagacaa agaaactaaa caaaatctcg aacaatattt
      661 agcactgatt gaatctccta ttgttttttc tgttagtttg gataatagtg aaaatagtca
      721 aaaactagct gaatttacga aggaaatagc agaaatgtct cctaaaatct cttgggaaaa
      781 aagagattcg atgcgcacac ctagtttttc tatcaatcca gttggcaaag aaagtggaat
      841 cgtttttgct ggtatccccc tcggtcatga attttcctct tttttactgg ctatgttaca
      901 aattagtgga cgggcaccga aaatcagtga atctttgagt cttaaaatca aaaaaataac
      961 acaaacattg cattttgaca gttatgttag cttgacttgt cataattgcc ccgacgttgt
     1021 acaggcattg aatatattag ctgtattaaa tccaaatatt actcatacga tgattgaagg
     1081 tgggatgttc caaaaagaaa tcgatgataa aaaaatcatg gcagtgccaa ctgtgttttt
     1141 aaatggagaa gaatttagta gcggacgcat gacgatcgaa caaattgttg aaaaaatcac
     1201 tggccctttg attgaagaag agttgtttga aaaagaacct tatgatgtat tagtgattgg
     1261 cggtggacca gcagctagta gtgcagctat ctatgcagca cgtaaaggaa tccgtacagg
     1321 acttgtcgct gattctttcg gcggacaagt cgttgaaaca ctagggattg aaaatatgat
     1381 tggtacctct tacacagaag gtccaaaatt gatgcagcaa gtagaaagtc atgtgcgtag
     1441 ttaccctatc gacattatga tgaatcaaca ggccgtttcc ttaaataaag agcaacattt
     1501 tattaatatt ggcttagcta acggtatatc acttaaagca aaaaccgcca tcatcgcgac
     1561 aggtgcgcac tggcgttcta tcaatgtacc aggcgaaaaa gagtttaaaa ataaaggaat
     1621 tgcttattgc ccacactgtg acggcccttt atttaaagga aaagagattg ctgttatcgg
     1681 tggtggtaat tctggtgttg aagcagcaat cgatttggct gggttagcaa aacatgtata
     1741 tgttctagaa ttcttaccag aattaaaagc agatcaaatc ttacaggata aactagtctc
     1801 tcttgcgaat gtcactgtta tcaaacatgc agcgacgaaa gaaatcagcg gtacgaatca
     1861 ggtagaatct ctctcctata tcgatcgccg tacgatggag atgcatacac tagctgtttc
     1921 aggagtattc attctagtcg gcttactccc taatacggat tggctagatg gaacgatcga
     1981 aatgaatcca agaaatgaaa tagtgaccga gaaaaatggc gcaacgaata tccccggcgt
     2041 tttcgcagct ggtgattgta cagatagtcc atacaaacaa atcattgttt caatgggttc
     2101 aggagctact gcagctttag gagcattcga ttatctgatg cgcacttgag ctatgaagat
     2161 tgtaaaaagt cagagagcgg aaaaaaggat ggtgacaaga atttt
//
LOCUS       DAZULY010000291         2145 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000291 DAZULY010000000
VERSION     DAZULY010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.296"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            103..1404
                     /locus_tag="ACG81A_002677"
     CDS             103..1404
                     /locus_tag="ACG81A_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS5015963.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(1473..2072)
                     /locus_tag="ACG81A_002678"
     CDS             complement(1473..2072)
                     /locus_tag="ACG81A_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5015964.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT          698 a          414 c          366 g          667 t
ORIGIN      
        1 ctgcaattta tcgagaagtc actttacttt gcctcttatt ctttcttcgt tacaataagg
       61 aatgggacaa gtaaaaaaat gaaaaagcaa gggacgaggt atatggaaaa aaagcaaaaa
      121 attattttag gagccacttc tgctttaact atcgttattt taattctaag tttgtttttt
      181 gtcatccgat catctacaaa aaaagaacca atcattcctg aaaaaaaaat aacagaaaaa
      241 gcaaaagaac cgacagatga taagcaacaa ctagttactc aagcaaaaaa tctggctcaa
      301 agctacgatt atgaccaagc gattgcgtta ttggaaaaag atagtaagaa cggatctact
      361 gaaacacaac agcttcttca aacactaaaa aaggagaaag aacaactgat cgactggtct
      421 gatcctactc agatctctca tgtctttttc cacagcttga tcgtggaccc gcaaaaagcc
      481 ttccactctc aacaggcaca aggatacaaa gattatatgg tgactgttga agaattcaac
      541 cgttcgatcg aacaacttta tcaaaatgat tttgttttag ttaatttaaa tgatttgatc
      601 caaaaagacg aacaaggaaa tctttccttt aacgggcttg cgctacctga aggaaaaaaa
      661 ccattggttc tttcacaaga tgatgtcagc tattatgaat atatggataa ttccggtttt
      721 cctagccgat tggtcgtatc aaataacaat gaaatcaaaa atgtctataa agagaaagat
      781 aaagaagaaa tcggtgactt tgacatggtt cctctgatcg atgcctttgt caagaaacat
      841 cctgattttt cttatcgagg cgcaaaaggt accttggccc tgacaggtta caacggagtt
      901 ttaggttatc gaacatcaaa atctcaatat ggagacaatg aaaaaacaaa aaaagagatt
      961 aaagaagcta aaaaagtggc ggaacagttg aagaaagacg gctggagctt tgcttcccat
     1021 acatggggtc acttgaatat gactcaagct tcactagaag atgtaaaaaa agacaatgaa
     1081 ttgtggcagc aagaagttgc acctatccta ggaaaaacaa atattctgat ttatccattt
     1141 ggtgcagaca tcagcgactg gcagccttat tcagcagaaa atcaaaaatt tgcttacttg
     1201 aagagtcaag gtttcgatat tttctgtaat gtcgatgctt ctacaccagc ttggggacaa
     1261 ttaggtacgg atttctatcg aaatgctcgg atcaatatcg atggcattcg ttttcaatct
     1321 gatttagacg gaaaaaatcc tattcttgat ccattcatca atgtccatga tgtctatgac
     1381 aaagaagcac gcgaatctgc ttgattttac gtcatttatg aaccaaaaaa gccgaactga
     1441 cattttatcg tcagtttcgg cttttttatg ctttatttct tcttcggtgc gattcccttg
     1501 atcaggactt ctgtccattc tgctgtatat ggaagcagtt ttgcgtatat ctccggctta
     1561 gaaattcctt ctaccattgc ttgaaaatag aggatcaaga actcatccga atatttcaaa
     1621 tccacttttc cttctttccg ccctcgatgg aaaagatcaa gcatgatact gaaactttct
     1681 tgcgtatacg catccatcgc gtcagaaaat tctttttttt ctttttttgt atagtatgtc
     1741 atcaaatcga tataaaattg tttgttcact cgattgagat aacggatttt gttttcactc
     1801 atggcaatca gtgtttcttc aaatggaaga ttttccgcaa tcaacgcttt cgcttcttgc
     1861 cctaaatcat tcataaatgc aaaaaatact tcataaatca gcccttcctt gctgccaaaa
     1921 tatttaaaaa tcgtcgcttt acttacctga gcgttctcag ctaccttatc cattgtcact
     1981 ccagcggttc ctatgtctgt gttcatcaag tcaaaagctg cttgtaaaat ctctttcttt
     2041 ttctcttgtg ctctcttttc aaaaccgttc atttgtagag cctcccgtaa ttttaaactt
     2101 ttataatata tatagttcaa atttattctg aggtttatta taact
//
LOCUS       DAZULY010000292         2103 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000292 DAZULY010000000
VERSION     DAZULY010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.297"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1309)
                     /locus_tag="ACG81A_002679"
     CDS             complement(<1..1309)
                     /locus_tag="ACG81A_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5015965.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILF"
     gene            complement(1438..2085)
                     /locus_tag="ACG81A_002680"
     CDS             complement(1438..2085)
                     /locus_tag="ACG81A_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS5015966.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
BASE COUNT          713 a          437 c          260 g          693 t
ORIGIN      
        1 aaaataaaat caatcctaca acaaagaaac aaactgctgg aatcaacgtt gaagagttat
       61 acagcttttg taaaacttct ccactttgta cttttgctaa cgaatcatac cctattatag
      121 aaagagtcca acctccgaca ccgccagcta aagcttgtcc aatttttcgg gcaaaagaat
      181 atactgcata aatagttcca tcttctcttt ggccagattt cacttcttga tcatcgatta
      241 catcagtaat attcgcccaa atgactgtat taaaaatacc taaccccata tatccaacag
      301 aagtaagaac taagaataca tacatattac taggctttat caaaaataaa agtaaatata
      361 caatagcaga gaagaacatc cctactgacg ccaattcttt tttaccgtat tttttactga
      421 aaattaaact tacaggtgaa acaagtacca acatcaaaat aggattgata aagttcatta
      481 aaactattcc ctgtgcatta ttataataat ctggaaatac ataattgttc atactcattg
      541 ttaaaagctg agcaagcaac aaaaaaatag ctgcagctat tatagaaagc aacgctctat
      601 ttttagctaa tccttttaaa gatttcaaca atgaaacttt taactgcgat tcatcacttt
      661 taacccgttc tacagttaat ttataacata ttacgtaaca aataactgca cacactgaaa
      721 atactcctgc cacaattgta aaagttgatc caccacgtac aatttgagct ccagaactat
      781 ctcttgtata aataaaaatt ggtgttaaca cgccaatgaa catacctgct aaggtagctc
      841 ctatactacg aaatgttgat aacgctgtcc tttcttctgc aacagaagtg atagctgatg
      901 ccattgaacc gtaaggaata ttaatagatg tataaaaaat acttccccat agtatatatg
      961 ttacatacat atatataact ttaatactca tagaagctga tgccattgat gtttgataca
     1021 ttaaaaaact tgctaaagcg actggaccac tcatccgtaa aatccacggt ttaaatttgc
     1081 cagttttaga tggttttgat ccatccacaa tacgccccat tgttatgtct gtaactgcat
     1141 caataaatct tgctactaaa aataatgttc ctacggtagc tccgcttatc cccaatactt
     1201 ttgtataaaa aatcattaaa aaagaactag caaaaatgaa agtgaaatca ttcccaaaat
     1261 ctccaaacat atatcctatt ttgtctctta gaccaaattt tctttccata taatttgcgc
     1321 tccttcctat tgttaagcgt tttcattctg ttgaactgcc aacttacgta ctatactctg
     1381 aagtataaag agttagtatt tattttttac attttatagt attatttatt ttttctccta
     1441 ataattcttt atctcataaa acttatctat atatttacta gctattcgag ttacttcttc
     1501 aaaatcacct ttttcaacag gagccaacaa attcccacct acaccaactg caacaacgcc
     1561 tgcttcaaac cactcttcta agttatctag acttactccg ccagtaggca ttatatttac
     1621 atatggtaat ggagcttgat acgtttttac aacagatgga ccaaaagcag aacctggaaa
     1681 tagtttaatg atttctactc cagcggtaag tgcctccttt atctcagtaa ttgtcataca
     1741 acctggtaaa taaggaattt gatataaatt acaaatctct gctgtttgtc gatcaaaaga
     1801 tggacttacg atatattctg ctccagccat aattgcaata cgagcagtaa acgcatctaa
     1861 aactgtaccg gctccaatta ttacatcttt ttgatcagca tattttttag caatttcttt
     1921 gattatttct tcagcattcg gaactgtaaa agttagttca atccctttaa ttccacccgc
     1981 agtaattgca tttattgctt taatggcctc atctttattt tttccacgta caaccgcaat
     2041 gactccagcc gcttctaact tacttaaaat ttctactttt ttcatatcta tctccttctt
     2101 tta
//
LOCUS       DAZULY010000293         2101 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000293 DAZULY010000000
VERSION     DAZULY010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.298"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(172..2007)
                     /gene="lepA"
                     /locus_tag="ACG81A_002681"
     CDS             complement(172..2007)
                     /gene="lepA"
                     /locus_tag="ACG81A_002681"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFS5015967.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT          570 a          469 c          402 g          660 t
ORIGIN      
        1 gtaagataac aaaaaatctg agccagaatg tggttcattc tgactcagat ttgattttgc
       61 acaactcatc agtccttatt gacaaagagc caaaaaacca ccggtggcaa accggtggca
      121 aatccatgta aacatggttt attttttaca ctgttttcaa acaaaccctt gctatttctt
      181 cggttcgtcc tcgtccattt tcagaacggc catgaaggct tcttgcggaa cttcgacgga
      241 gccgatttgt ttcatccgct tcttcccttc tttttgtttc tctaagagtt tacgtttacg
      301 agaaacatct ccaccataac atttagccaa gacgttttta cgcaaggctt tgatgtctga
      361 acgggcaacg attttttgtc caatagctgc ttggattggc acctcaaatt gttggcgtgg
      421 gattagtttt ttcagttttt caacgattgc ttttccacgt tcataggcaa agtctcggtg
      481 aacgataaag cttaatgcat cgactttttc tccattcagc aagatgtcca tcttcactaa
      541 cttgcttttt tgataaccag acatttcata atccaatgag gcatagcctt tcgtgcttga
      601 tttcaatttg tcgaaaaagt caaagacaat ttcagaaagc ggaatattat agacgacatt
      661 tactcggtaa tcatctaaat aatccatcgt aatgaattct ccccgttttc gttgagaaag
      721 ttccattact gcaccgacaa aatcatttgg gaccatgatc tgtgctttga cgaatggttc
      781 ttctacatcc tgaatcgtta caggttcagg aaaatctgcc gggttatcta ctgtagcagt
      841 cgtaccatcc gttttattaa catggtaaat aacggatggt gcagtcgtga tcaattctag
      901 attgaattcc cgttccaagc gttcttggac gacatccata tgaagaagtc cgaggaatcc
      961 acagcggaac ccaaaaccta atgcttgaga ggtttctggt tcgaattgta aagcagcatc
     1021 attcaattgc agtttttcta aagcttcccg caaatcgttg taacgagaag tatcaattgg
     1081 ataaagacca caataaacca tcggattcat ttttcggtaa cctggtaaag cttcagcggc
     1141 aggattgtct gctaacgtta ctgtatcccc tactcgtgta tcttggacag ttttgatact
     1201 tgctgtgata taaccgacat caccgaccat caaaaagtct ctagcaactg cttttggcga
     1261 gaagaccccg acctctgtaa catcaaatgt tttgccatta ctcatcagtt tgattttgtc
     1321 tccgggtttg accataccgt ccgtgatccg gacatttaga acaacaccgc ggtaactatc
     1381 ataaatagag tcaaagatca aagcttttag cggtgcatct aagtctccac ttggtgcagg
     1441 aacatattcc acgatctgct cgagaatatc ttcaatccca atccctgatt tggcactggc
     1501 taatacagct tcactagcat cgattccgat tacgtcttcg atttcttgac ggacacgttc
     1561 gggatctgct gcgggtaggt cgattttatt gatgactggc aggatctcca aatcattgtc
     1621 taacgctaaa taaacattgg ccaatgtttg tgcttcgatt ccttgtgctg catcgaccac
     1681 taaaacggca ccctcacagg cagcaaggct tcgagagact tcataggtga agtcgacgtg
     1741 ccctggggtg tcaatcaagt gaaagatata tgtttctcca tttttcgctg tgtaattgag
     1801 ttctacagca tttaatttaa tcgtgattcc acgttcacgt tccaaatcca tcgagtctaa
     1861 cagctgatct tgcatttcgc gagacgtcac agtgtgcgtc atttctaaaa tacgatcggc
     1921 caatgtagat ttcccatggt cgatatgagc gatgatcgaa aaattacgaa tctgctcttg
     1981 gcgttctttc attttcttta tatccatagc gtctcctctt ttcatataat cagcactttc
     2041 cattatacca acgaaaagca gtaatttaaa gagtttttaa ttagtcagct atagaagttt
     2101 t
//
LOCUS       DAZULY010000294         2098 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000294 DAZULY010000000
VERSION     DAZULY010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.299"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>2098
                     /gene="acm"
                     /locus_tag="ACG81A_002682"
     CDS             <1..>2098
                     /gene="acm"
                     /locus_tag="ACG81A_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFS5015968.1"
                     /translation="IILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVDPTNITD
                     GGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRK
                     DSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPA
                     SGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFE
                     INQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYK
                     TIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKY
                     INDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPN
                     WIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLF
                     RASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSG
                     YQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSE
                     KDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSH
                     ITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSIL
                     PKTSSKKSSFAVIIGLL"
BASE COUNT          821 a          343 c          406 g          528 t
ORIGIN      
        1 attattttat caatgttatt tattattact aatataacat cactgattcc tgttcatgtc
       61 tatgcggatg caggcagaga tatcagcagt aatgtcactt cgttgaccgt ggacccaacg
      121 aatattacag atggaggaaa cataaaagta aaattctcgt ttgacgaaaa aaaacaaaat
      181 atccaaccag gtgattatct ttggatcaat tggccaagtg aaggaaatat tcgcggtgaa
      241 ggattccaaa aagaaatccc attaatgatt gaaaataaaa atgttggaac tttaacggtc
      301 agaaaagatt ctgcgcaggt tgtgtttaat gaaaatatta aaaacctaga ctcagtagaa
      361 ggttgggggc aatttgagat acaggccaga aacgtaaccg ataccggtga agaaaatacg
      421 ggaccattca ccgtgacgag cggtgataaa acagctactg taaatgtgac aaagccagct
      481 tctggttctt cttctagcgt tttttattat aaaacaggag acatgctacc tgaagatact
      541 aagcacattc gatggttttt gaatattaac aataacggca cttatgttga acaaccagtt
      601 aaaatcagtg atgaaatcca aagtggacaa agattagatc caagtacgtt tgaaattaat
      661 caaatacact taggtgaaca gaaggtttat agaggagaag agggaatcca acaattttta
      721 caagatttcc ccagcgctac atttaatttt tcagtaactg ataattacat tgaaataact
      781 ataccaaaaa actttgtcaa tcttaggaaa ataatggtga gttataagac gatcatagaa
      841 aacccagaac aaataaattt tgagaatcat tcggaagctt ggtttaaaga atttaataaa
      901 cctgctgttg atggagaaag tttcaatcat acagttaaaa atatttcagc ttctggtgga
      961 gtcaacggta cagttagagg cgaattgaag attttcaaat atatcaatga cacagagatt
     1021 ggtatcccaa atgtgacttt cgaattaaga cgagcagatg aacagcctat tcaaggacag
     1081 tctagtatcc tgttgacttc gaatgaacag ggagaagcta gtatcaaagg cctgcaagta
     1141 ggagactatg ttgtaaaaga aaaagaagca cccaattgga ttgattttga tccactaagt
     1201 tcaaatgaac tcaaattctc cataaacgaa aatgatacag agggagtcag tttacctatt
     1261 tataacaaga aaaaggttac gaacatcaca gcaactaaaa tttggaacgg tggaaccact
     1321 ccaagaccga gtatttattt taaattattc agagcaagta caaacaattg ggaaccagtt
     1381 cctgatgcag aaacaaaacg attagacaat ggaataactt cagtgacttg ggaagatatc
     1441 caacagtatg atgatagtgg aaacgaatat acgtttaaag tgcaagaagt agatcaaaac
     1501 ggaaatgatt atgtcccttc aggttatcaa aaaattgaaa acgggctagt cgttacaaat
     1561 gagaataaag aagtcatttc agtctcagga caaaaaacat gggaagacaa tgagaaccaa
     1621 gacggcaaac gaccgacgac gatcacagta aatctattag ccgacggtca accgatccaa
     1681 cacaaagaag taagtgaaaa agatgattgg cgctatcgtt tcacaaatct gccaaaatac
     1741 agagacgggc aagaaatcat ctatactgta acggaagaca acgtgcccga atacagtacg
     1801 acgattgacg ggtataacat caaaaacagt tatacaccgg aaaaaacaaa tatatctata
     1861 ccaggtagcc atataacacc gtggaaaaat tttccagaga agacagagag agagaaaatt
     1921 agaaatctat tctctcaaaa attgcaaata tttgctcata caggagaatc cagcgaacaa
     1981 agaagcaatt atattagtaa gagagctgta cctaaacaga taacaaataa atcaaaaagc
     2041 attcttccta aaacaagtag taaaaaatct agttttgctg taatcattgg tttattga
//
LOCUS       DAZULY010000295         2065 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000295 DAZULY010000000
VERSION     DAZULY010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.300"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1008
                     /locus_tag="ACG81A_002683"
     CDS             <1..1008
                     /locus_tag="ACG81A_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015969.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="ACG81A_002684"
     CDS             1025..1174
                     /locus_tag="ACG81A_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015970.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="ACG81A_002685"
     CDS             1171..1617
                     /locus_tag="ACG81A_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015971.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="ACG81A_002686"
     CDS             1619..1756
                     /locus_tag="ACG81A_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS5015972.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2065
                     /locus_tag="ACG81A_002687"
     CDS             1794..>2065
                     /locus_tag="ACG81A_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015973.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          753 a          358 c          425 g          529 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa acttt
//
LOCUS       DAZULY010000296         2061 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000296 DAZULY010000000
VERSION     DAZULY010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.301"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            674..1006
                     /gene="traE"
                     /locus_tag="ACG81A_002688"
     CDS             674..1006
                     /gene="traE"
                     /locus_tag="ACG81A_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFS5015974.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1625..1966
                     /locus_tag="ACG81A_002689"
     CDS             1625..1966
                     /locus_tag="ACG81A_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFS5015975.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          717 a          275 c          433 g          636 t
ORIGIN      
        1 gccttttacg tcataagtgt taaaagaaat aattaatttc aaatatagaa aagggaacgt
       61 atgtttgcat actccttttt tttgagttat aataatacgc gaagagagat aatttcatta
      121 tttaaacaag ctaaaaatct tgatattact tcttttcagg caatacgaat attattcgct
      181 tacatttttt gtataaattt aattgtctgc tatgcttgtg atgaattgat tttcgttttg
      241 cacttttgga tgataaattt ggacaattga aaatacaaaa aaaagaaagg cggtatgaac
      301 tcggaaggct ggtaaccttc gtcagcgttc atacacggca ttaagatttg gcccttaatg
      361 cgttaacctt tcagtgttac ggtattaccc gcaatggatt tagtataaca gatattcaaa
      421 agtgatacca ctttttttgg ctgtgttaag gttaaaaata cgtgatatta cgatgaatct
      481 atgcctaagc ataatgttat aaaaatgtcc gtgtggctct ttgccgtatg acgaacgctg
      541 aaattagagg gaacgcatgt ttggatgcgt tccctctttt ttgtttgtca tgaacgaaaa
      601 tcaattctct atttttgaaa aataacaaga aaaacttgtt aaaaatattt aaagaaattg
      661 ggtggtttat tttatgtcat atacaggaat tttgagtctc aaagatattt gtcactacgg
      721 taaacggtgt accgctacag agaaaatcac gaagaaacta tcaactggcc aaagtaaaac
      781 cgttgtgcag tgtaagaaat atataatcca gaaagacaaa gtctcagagg aaatgattta
      841 ttacattggg aaacaaaagc aaataatttt aaaagatcct atccccttaa aagagctata
      901 tccaacaatc aaacatgttt atgaccaaaa cggtgtactg attggtcgaa gaaaaaatgg
      961 agtcttgcgt tgtaccgcaa aagggatggg gcgtttgatc ggctgaattg atcattctca
     1021 taacttgtat tttatcgttt ttcgcttgct tgaaaatatt gtcgagactt ctaaaggaac
     1081 ttagagtcac tagaaaaata aggagtagac ttgatctatc aacaattccc ttcggggagt
     1141 gacggggtag gttttaacga ttggtaacgt aaaaaattgg tcaatcgagt aaatgcgctt
     1201 gtttggatag tggtgaaatg aatttgtgta aataataagc gcagagtaaa caagagggaa
     1261 tttagaagtt agacaagtta gaatagcgtc tagattaatc atgagcagct actaagggat
     1321 ctagactttg acgcaaactc atggaataag gagtaggtct tctcgtaatg gggaggacgc
     1381 acaagaaatt gtgtcgtgtg ctgcagtaca tgtagatact tgcgaggacg ttgattattg
     1441 gatcaatcga gcatttaccg ctgtctacta gggtttctcg tttcggacgc cataaacgag
     1501 tcgaaatttt ttatgtattt ttaagtaaac gaaaaacgat aaaatatgaa caagtcctga
     1561 ataaagtgaa aaaattaata taaagcatag agaaaatgaa atttacgtaa tggaggaatg
     1621 acaaatgaaa aattatgatc cgaatatacg attgggcaca catactatta aggtttcttt
     1681 ccaacgttgg gattacaaag gatttgtgac gtttagaagg ggcggaaatt gtaaaggatt
     1741 agatgtttta gcattagacg aggatgattt atacgatcaa acattgactg ataatccaat
     1801 tggattcggc ttgctacctg aagatgatga aggtaatgaa tggtttaaaa tgacgcttat
     1861 gaatgataat ggagacgaat tatctgttga agatacttgg tcatatttga gtgattatat
     1921 tgtcagcttt gaaatcattg aatttgtagc ggataaagaa gaataaaaac tggaggaata
     1981 aattttacag ataaaggctt agaaggagtc acgacgatta ataaaaaggt agtattacct
     2041 taggcattga gtaaggaatt t
//
LOCUS       DAZULY010000297         2055 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000297 DAZULY010000000
VERSION     DAZULY010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.302"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..365
                     /locus_tag="ACG81A_002690"
     CDS             <1..365
                     /locus_tag="ACG81A_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS5015976.1"
                     /translation="CVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNKIR
                     HSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIHSS
                     IHYLTPQEFEELAKEKMA"
     gene            complement(721..1293)
                     /locus_tag="ACG81A_002691"
     CDS             complement(721..1293)
                     /locus_tag="ACG81A_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5015977.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1309..1914)
                     /gene="fic"
                     /locus_tag="ACG81A_002692"
     CDS             complement(1309..1914)
                     /gene="fic"
                     /locus_tag="ACG81A_002692"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFS5015978.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          638 a          425 c          308 g          684 t
ORIGIN      
        1 attgtgtcat tcaaacattg aataaagcaa aaatacatta tcacattcca gaaggaatga
       61 ttctacacac tgacttgggc agccaataca cagcaagaga agtggaacaa tggcttaaaa
      121 ccaacaaaat aaggcattct tatagtagaa aaggaacacc ttatgataat gctggaatcg
      181 aatctttcca tgcctcattg aaaaaagaag aagtctacac gactagctac tcagattttg
      241 aagaagcaaa tcgagcgcta tttagctaca ttgaaggatt ttataaccga aatcgaatcc
      301 atagctcgat tcactatcta accccacagg aatttgaaga attagcaaaa gaaaaaatgg
      361 cttaaccgtc tctactaaag acaatcaaca ccgtagggat atggagataa aatcaataga
      421 gtcaatagat tatttatcga taataacact aaaaaagcca cgttcctaaa cgtgacttac
      481 gctgaacgat atgtgttgtt tggtagacga acaaatcgtt cattataaat atatgatggt
      541 atcctaatat agtaagcgtg gctagcatac ctttaattac tttacctttt acagttacca
      601 tacacatttt ttgagcagaa atcaagtaaa taaaaaaata ttatatttca caacatgcta
      661 atagactcat atctagaacg cattaagtat ttacctgtct ggttcgctga gacttctttt
      721 ttaactttct tcaattttct tctttattct aaaaagcgta ctgcgactga aaccggttat
      781 agcttcaact tccttatacg atttacctga agtaagcagt tcatatgcgt gcctcttctt
      841 cggtgtgatt gttgctttcg gacgtccttc tctgaaatta ggattattct ttcttgcaaa
      901 catttttcct tcttgggtac gttccacaat catatcacgt tccatttctg ctacactcaa
      961 caacgtccgt gtcaccattc gccccatgga cgtattttca atcgttccta gattcaaaac
     1021 cttgatcgtt acgttatcat cgagtaaagg ttcaatgatc tctaatgctt ctctcgtgtt
     1081 acgagcaaat cgatcaagct tcgtaacaac tatttcatct ccaggcttca gaccagccaa
     1141 cagctgattg aattttggcc gatcccgttt tgtacccgta aatttttctg aaaaaatatt
     1201 cttcttctct acgcagttct caagcaactg ttgaatttgt tcttttgtac tttgagaagc
     1261 ggttgagaca cgtgcatatc catatttcgt catttggtcc acctactttt acaaatcatc
     1321 tatcgtatac tcagaatacc cttcatagaa atagctgaca tcgattcctt tcatatataa
     1381 ctcgcgatcg tcaattttat cagtcagagc attggaaatt agatagcgta tttccaaatc
     1441 gtttatggga cttcgctcca ttgcagaaag atagtctgct ttatcgatta agttccaatc
     1501 tacaactttt tgcaattctt cctttaaaat gagatccagc caaattctag tacttcggcc
     1561 atttccttct cgaaatgggt gagcgatatt catctccaca tatttcttga taatctcttc
     1621 aaaagaagtt tgtggcatct gatcaatatg ctgtaacgaa tgttctaaat acataactgg
     1681 tgcaaatcga aaatttcctt ttgcaatatt gaccgatcgg atttttccgg caaaatcgta
     1741 aatatccgaa aatagaaatt catgaatttc agataaacct ttaaatgtac caacttcgat
     1801 ttcatttatt tttccacaat cgtacaattc tttcgctttt tgtttactga gtttttcttc
     1861 aaccttagcc aactcaactt gattggttaa tcccaattta ttctctaaaa tcatgcgaaa
     1921 tctccttaac tttatgacat aagtttatga aacactctca accccattat atcgcgattt
     1981 tgtttatgtg tcaaaaacta ttaagtttct gacccccgat agatttttca agtgtctcta
     2041 ttttgcacag tctag
//
LOCUS       DAZULY010000298         2021 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000298 DAZULY010000000
VERSION     DAZULY010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.303"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..690
                     /locus_tag="ACG81A_002693"
     CDS             190..690
                     /locus_tag="ACG81A_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFS5015979.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..>2021
                     /locus_tag="ACG81A_002694"
     CDS             787..>2021
                     /locus_tag="ACG81A_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFS5015980.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMIS"
BASE COUNT          648 a          275 c          458 g          640 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt c
//
LOCUS       DAZULY010000299         2009 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000299 DAZULY010000000
VERSION     DAZULY010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.304"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            32..1021
                     /locus_tag="ACG81A_002695"
     CDS             32..1021
                     /locus_tag="ACG81A_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS5015981.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            1310..2008
                     /locus_tag="ACG81A_002696"
     CDS             1310..2008
                     /locus_tag="ACG81A_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFS5015982.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT          701 a          324 c          430 g          554 t
ORIGIN      
        1 ttttaaaaag atttgatgag gaggagaaaa attgtatatt gcagatagtg cacgttatga
       61 aaaaatggaa tatcgtaaat ccggtcattc aggtttgaaa ttaccgattt tatctctcgg
      121 attatggcaa aattttggtg attatgatcc tatccataat caaagagaga ttttaagagg
      181 cgcattcgac atgggaatta cccatttcga ccttgcgaat aattatggag gccctgctgg
      241 agctgcagag aaaaactttg ggcgtatttt tagagaagat tttcaagcct accgtgatga
      301 actgattatt tctagcaaag caggttatca tatgtgggaa gggccatatg gggaatgggg
      361 ctctcgtaaa tccatcattt caagttgtga ccaaagtctc caaagaatgg ggctagatta
      421 tgttgacatc ttctatcatc atcgcccaga ccctgataca cctttggaag aaacagcaga
      481 agctttgatg caacttgtaa gacaaggaaa agcattatat attggtatat ccaactataa
      541 tggagaagat acaaaaaaaa tgactgaaat tttaaaaaga aaggaagcac cgttcattat
      601 tcatcagatg cgctacaata tgttttctcg tgcactacta gaagacgatc tatctccagt
      661 attagaagaa gaagggctag gggcaatcac gttcagtccg cttgctcaag ggttgctgac
      721 aaatcgttat ctacatggta ttcctgaaga ttctagagca catcgtaagg aaataccatt
      781 cctttcagaa gaacaagttg gatcaactct ggaaaaaatc aaagcactgc aaacaatcgc
      841 cgtatcacga ggacaatcat tagcgcaaat ggcccttgca tggaatttgc gacaaaaatc
      901 tgttacaagt gtactggtcg gagcaagtcg gttaagtcag ttacaagaaa gtgttcgaat
      961 gatggataat ttagatttct caccagaaga agaattgaga atcgaccaaa ttctagaata
     1021 gaaacgatgt aatcaaactt tttatccata tattaaaaaa aacaaaagat gacatcaaga
     1081 atcgaatgaa gatcgtctgt tattgaacat catatttttt gtcgttcaaa ataaaaatga
     1141 tcgaaggcta ggagtgaatt ttatcctagt cttttttatg gtaaatatct tcttcgtttg
     1201 tatgtctatg aacggattaa tcaattttgt atacaaaaaa tcatcagatg attcaaacgt
     1261 gttataataa ttacgaaaaa atacatcaga tgaaaaggag gtatgtggtt tggaagaaaa
     1321 taaaacccta gtagaagtaa ctactgacaa gatccttaga tatttaaagg aaatgcagtt
     1381 gcaagtagga gataaactgc cgaatgaata tgaattgtcc gattatttag aggtggggcg
     1441 aagcacggtg cgagaagctg tgcgggctct agcttccagg aatatccttg aagttcggca
     1501 aggagcgggg acatttgtca gtgataagcg agggattgcc aatgacccac tcggtttttc
     1561 tttgattgaa gatcaggatc ggatgatacg agacttattt gaactgagat atttattgga
     1621 gcctaagatg gcaggtttgg cagcggaata tgcaacgaag aatcaaatcg tccaaatgaa
     1681 aaaattaacg caaaagattg aaacttcttt tcgagaaggc acacaagaac atgttcaatt
     1741 agacatcgat tttcatacga tgattgcaga agctagcgga aacgtagcct tgctgcatat
     1801 tatcccaatc attaatgaat caattgcttt gtttaataaa aattatgata tccagcatct
     1861 gaaaatcgag acgatcgata ttcatacaga gatcgttcag gccatcgaaa ggcggaatgc
     1921 acttgctgcg tttgatgcaa tgactgtcca tatggcaata aatcggagag aattacagaa
     1981 aataatagga gaaaaagaaa ctaattgac
//
LOCUS       DAZULY010000300         2007 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000300 DAZULY010000000
VERSION     DAZULY010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.305"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1872
                     /locus_tag="ACG81A_002697"
     CDS             <1..1872
                     /locus_tag="ACG81A_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFS5015983.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          733 a          321 c          412 g          541 t
ORIGIN      
        1 agtgctgaaa atatggaaga aatcaaaaaa atgtttccga aatccacttg gtttggcttt
       61 acaggtacac cgatttttcc tgagaatcgg aaacaagcca aagggcaatt ggcacgtaca
      121 acccatgatc agtatggaga ggtcttgcat acctatacca ttaagaatgc gctagaagat
      181 ggctctgtct taggatttca agtagagcat gaaaacaccg tggagccgac ttcactagag
      241 aataaaatct atcggaaatt aaaagaagta gaaacatatg cagagtattc atcagaacag
      301 atcaatcgta tgattgatca aatggaacct gtcaaaaaag aaagttattt ggacccagct
      361 gtttttgaag cagatgaaca tatccaaaaa gtcattcata aaatgttccg tccagataat
      421 gcctatacaa aatttgattt tcgaaatggc aggccaacga aatctgcaat cttaaccacg
      481 agttccattg acatggccaa aaagtattat cgggccatca aagagatgac gaaagagcct
      541 gattggttaa caaaagagtt tcccaatcag ctcattcgtg aaggacgaac gatggaggat
      601 cctgattttc cacgaatcgc tattacctat tcattagaag aaaatagccg agattcaagt
      661 gcccaacaag aagaaatgca aaaaattatt gaggaatata atcagtatta tgatacttcc
      721 tggtcattag cagacattga gcgttataac ggagacatca ataatcgttt ggcacgaaaa
      781 agagcggaat tcaaacaatt tgggaaacaa atcgatttag cgattgtagt ggatcgtcta
      841 ttaacaggtt ttgacgcccc tacgattcaa accctatttg tggatcgtaa tctagagtat
      901 gcaggattga tccaagcttt ttcacgtacg aatcgtacgt atcctgataa gacaaaagga
      961 ttgattgtga cgtttcgtaa gccagcgaca atggagaaga atgtcgagga tgcgacaaaa
     1021 ctgtattcaa aagcaaaaga agaatcaggt ttgatttatc caagttatca ggaatccaaa
     1081 aaaagattca aacaagcata tggtaagtta aaagaggttt catccatgga atccattgat
     1141 gaacatacgc cacttgaaat ccgtatcgag tacgtcaaag catttcaaga attgaatcgt
     1201 gcttatgaat cgttagtgac ttatgacgat tacaatgatg acatggtgaa atctccaact
     1261 ttgaacaatc aaattcaact tttggaagaa caaattggcg tgtacgagac cgtgaagggt
     1321 tcattaatag aggaagaacc agaaaaagaa ggagaagatt tttcaacgat cgaattttat
     1381 agtgaaaata ccactaagtt gtatgatatt gattcaactt atattgatca attactagga
     1441 acttatgctg cgaatagttc agatgcacga gaagaaattg aaaaagcttt ggctaaattg
     1501 aataaaacag aaggagtcaa agaagtttac cgacaaattt taaatgcatt tgatgtgggg
     1561 actttagata gtaatgaaga tatctttgtc attaaacgtc gctattttac acaagcgagt
     1621 gatgatttga ttcatgtctt ttccaatgag tggtttgttt ctgagaaaga attacatgct
     1681 tctaatctgc aatatctacc aggtgaagat ccaattccta atatgaaagc aattatcaac
     1741 agtaaggatt acgaaggata taaagccaaa catccagaag cgaagccatt taaataccca
     1801 caagaaatga agcgagcatg gcgtaaaatg ttagatgatg agttgattcc acttgagaat
     1861 gaattgaggt aaacgagaag agttaagaaa attgtacgat tcaaagatgg tagtcaagtg
     1921 gaagtgtaga aaatagggat agatgtcttt tatttgttca cattttataa cgaagtattt
     1981 aagacagcta ttgaataatg tttcctc
//
LOCUS       DAZULY010000301         2004 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000301 DAZULY010000000
VERSION     DAZULY010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.306"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACG81A_002698"
     CDS             <1..378
                     /locus_tag="ACG81A_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS5015984.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACG81A_002699"
     CDS             426..1826
                     /locus_tag="ACG81A_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFS5015985.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          340 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt ttac
//
LOCUS       DAZULY010000302         2002 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000302 DAZULY010000000
VERSION     DAZULY010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.307"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..1251
                     /locus_tag="ACG81A_002700"
     CDS             25..1251
                     /locus_tag="ACG81A_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFS5015986.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(1359..1829)
                     /locus_tag="ACG81A_002701"
     CDS             complement(1359..1829)
                     /locus_tag="ACG81A_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFS5015987.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT          740 a          309 c          394 g          559 t
ORIGIN      
        1 gaaaatgtga aacagaggtg tattatgaag cgatggcaaa aagttctatt aaccttgtta
       61 ggggtattcg ttttgctgat tgccggatta tcagcatacg gaatcagatt tatgggagaa
      121 gcgagccaaa cagtaaacaa aatttctaaa aactctaatc gaatatcctc caaacgttct
      181 gagagagtaa gcattgatga caaagaacct ttttcggttt tgctattagg tttagatacc
      241 ggtggattag gaagaacgga acaaggacgt tctgatacga tgatggtagt aacagttaat
      301 ccgaaacaaa aaaaatcaac gatcatcagt ttggaccgag atatttatac gaatatcgta
      361 ggttatggaa cggtagataa attaaatcat gcctacgcat ttggtggtgt cgaaatggcc
      421 atggattcaa ttgagcaact gctcgatatt cctattgatc attatgtaac gatcaatttg
      481 gatggcatgg aagatttgat caatgcggtc ggtggcatca aagtgaataa taaaattgac
      541 tttactttgg atggtgtcca tgtaccagta ggtgaacaaa cattgaatgg agaaaaaggt
      601 ttagcctatt ctcgaatgcg acacgaagat ccagaaggcg atatcggtcg acaacgtcgt
      661 cagcgggaag tagtgacgaa aatcgtcaat aaagttttaa gtttagatgg cgtaagcaat
      721 tatagaaaaa ttttaaaagc agttgagaac aatgtgacga cggacttaga ttgggatgac
      781 atgcttgatg tagcgacgaa ctacaccccg gcgtttgaaa caatcaaaca agaccagttg
      841 caaggcgaag gacaaatgat caatgatatc tactatcaaa ttttaggaaa aaatgatctt
      901 ctttcgatcc aaaatgaact gaagaaacaa ttaaatataa aaacaagcga tacattacct
      961 aatttgaaaa atgagaatgc ttcaatgatg ttctttgatg attcggaaga tggcgattcg
     1021 acaaacggtg atggatcgaa tagtgaaggg tacaccgatt attcttccta ttcagaaaac
     1081 acatatgatc agtaccaaga tccgaatacc tattatcagc aaacagaaga tacaagtcag
     1141 tactatacac caagtcaaga tactcaaagt tatgatcctt acgctggaca ggaacaaaat
     1201 ggttatggtt atgaccagaa taatgagtat ggaacaacaa gtgggtacta gaaaaaaata
     1261 gtttggttaa taaagagtgc cacctaaatt agacaaaaaa tctaacttag gtggcacttt
     1321 ttctatgtgt taaattacag gacgtctctt tttagcgctt agtttaaaaa ataaattgaa
     1381 taattcaata gagttgcgaa tagtacccat aaataataag gaatcatgag ccacattgtc
     1441 aggcgatgat gcagccacaa ctgatacata agcactgaca agaaaataac taaaagtgta
     1501 atatcgattg ctgcaatgaa aatatttctt aaattaaaaa atagagcaga ccatataaaa
     1561 ttaaaaatga actgaatcac aaaaagaccg acaagattgt ttcgctgttt tttgttgttg
     1621 tgattaagta acaaataaag agaaataccc atcaatatat acagtaaggt ccacatcggc
     1681 ccaaatatcc agctaggtgg tgcaaatggt ggaagttgca aagaatagta atattttccg
     1741 ggatttccgc taagcaaccc tgataaaaag cctaatatga caattcctat aacacaaacc
     1801 caaaaacgaa tatcttttat catcttcatt ttactaactc ctttctataa tattagttta
     1861 ccatgaaggc tcacagcaag acctgtattt agtttgtgaa gtaagaaaaa aattgataat
     1921 tgttgttatt aaaaagttga acatttgttt atatgaatga tgtttttgaa aaagatcata
     1981 tgatttgatt ctcatgtttt ct
//
LOCUS       DAZULY010000303         1993 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000303 DAZULY010000000
VERSION     DAZULY010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.308"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACG81A_002702"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACG81A_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFS5015988.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACG81A_002703"
     CDS             852..1040
                     /locus_tag="ACG81A_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFS5015989.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACG81A_002704"
     CDS             1053..1610
                     /locus_tag="ACG81A_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFS5015990.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACG81A_002705"
     CDS             1642..1884
                     /locus_tag="ACG81A_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS5015991.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          308 c          406 g          555 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc ctt
//
LOCUS       DAZULY010000304         1968 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000304 DAZULY010000000
VERSION     DAZULY010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.309"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACG81A_002706"
     CDS             complement(<1..914)
                     /locus_tag="ACG81A_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFS5015992.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACG81A_002707"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACG81A_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFS5015993.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          652 a          362 c          385 g          569 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcgg
//
LOCUS       DAZULY010000305         1962 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000305 DAZULY010000000
VERSION     DAZULY010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.310"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..272)
                     /locus_tag="ACG81A_002708"
     CDS             complement(<1..272)
                     /locus_tag="ACG81A_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015994.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
     gene            complement(309..446)
                     /locus_tag="ACG81A_002709"
     CDS             complement(309..446)
                     /locus_tag="ACG81A_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFS5015995.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(448..894)
                     /locus_tag="ACG81A_002710"
     CDS             complement(448..894)
                     /locus_tag="ACG81A_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015996.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(891..1040)
                     /locus_tag="ACG81A_002711"
     CDS             complement(891..1040)
                     /locus_tag="ACG81A_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015997.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            complement(1054..>1962)
                     /locus_tag="ACG81A_002712"
     CDS             complement(1054..>1962)
                     /locus_tag="ACG81A_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015998.1"
                     /translation="LLDDPYWVGKTSLGENIANKGTTFPIKSSAYEIYKGNTEEELII
                     GQTYTITLKGTKPASQTFVAYNYWNVNFGELKPVEGLTDVWSLTFTPTKLEPGLPKDL
                     RIFQLPKETAGACQIDWLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYT
                     EKGLNDTVSLTEPQSIEGLKNFEDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKL
                     YREGKTVTIYFFALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSAYSFVITYSI"
BASE COUNT          510 a          407 c          341 g          704 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcatagtg gaacgaatag tcgaacttga tcgtcagtaa aacagcccca atcatacatc
      421 attttcacat cgctaaaact aaacatacta ctcacctcct tttgaagctg gatttagttg
      481 ctctttaatt tctgaaatgt ctttgctatt ttgtaacgaa gcaagcatca tttttgaatt
      541 gatttgtgct aaactgtcag ctttttcttt caatgcagta ttttcctgtt taatcgctac
      601 atcgcttaac atgagtttgg catttagctg ttttaggttg ccgttttcat gttcaagagc
      661 ctcgtacatc gctttgagat tgtttaaatc gttgtgatct aacgcgttcg ctaaaataat
      721 ccattggttc aatttaggat caaacatctg atcagcaatc gttagcggtt cgccatctga
      781 acgaacccct tcgagcggtg gctgatctgt gtaaggaacg gatacaagca tgtcgtccaa
      841 tacttttcct gcatactctc cgccagtacg gccatatttc caaatgtttt tcatttattt
      901 ccctcccagt aattgaattt cggtttccaa tttggaatcg gtggttcgac ttctgtgcat
      961 tcttccggta aatgttcttc gtcattcaca atgatttgct cgaatccgta aggcttgatt
     1021 gatttgtaaa ctactttcat tagatttcct cccttaaatt gaataagtga taacaaatga
     1081 ataagctgat ccataacttg agtttcttcg ccatttaatg gctccatctg caccaataga
     1141 taactgagca ctgttcaaag tagaacggtc tatcgagcca accagttgct caaaactaat
     1201 tggtggccga tagccttctg gaattgttag tatcgttgaa tcatttccac cactgctttt
     1261 cccattcaac gcaaaaaaat aaatagtgac ggtttttcct tcacgataaa gctttgctga
     1321 tccggtattc ccgtttgtaa ctgttaaggt tacgacttca tatttgttgt cgtctgatgt
     1381 caatacagat tttccttttg attgaatacc atcttcgaaa tttttcaatc cttcgatgga
     1441 ttgcggttca gtcaaactaa ccgtatcatt caagcctttt tcagtatatt caggcgtgat
     1501 gtcccagctg tagtcgttcg gattgttgct atctttcaat ccttcaccaa agtatttaaa
     1561 ctgactaata ttcggggttc gggtgtcgcc tttttcaatt ttgagccagt caatttggca
     1621 tgcgccggcc gtttctttag gcaactgaaa aatgcgaagg tctttaggca aacccggttc
     1681 aagtttcgtt ggtgtgaatg ttagagacca tacgtctgtc aatccctcaa ctggttttag
     1741 ttctccaaaa ttaacgttcc aataattata tgctacaaag gtttgacttg cgggttttgt
     1801 tcctttaagc gtgatagtat acgtttgacc tattataagc tcttcttccg tgttaccttt
     1861 atatatttcg tacgcgctag atttaattgg gaacgtcgta cctttatttg caatattctc
     1921 acccaacgat gttttaccta cccagtaagg gtcatctagt aa
//
LOCUS       DAZULY010000306         1951 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000306 DAZULY010000000
VERSION     DAZULY010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.311"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..302
                     /locus_tag="ACG81A_002713"
     CDS             <1..302
                     /locus_tag="ACG81A_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5015999.1"
                     /translation="FIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVITLLLLVMM
                     LSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINPLIFSR"
     gene            466..720
                     /locus_tag="ACG81A_002714"
     CDS             466..720
                     /locus_tag="ACG81A_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016000.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            790..1035
                     /locus_tag="ACG81A_002715"
     CDS             790..1035
                     /locus_tag="ACG81A_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5016001.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1111..1476
                     /locus_tag="ACG81A_002716"
     CDS             1111..1476
                     /locus_tag="ACG81A_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFS5016002.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1537..>1951)
                     /locus_tag="ACG81A_002717"
     CDS             complement(1537..>1951)
                     /locus_tag="ACG81A_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016003.1"
                     /translation="LSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGL
                     NRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRR
                     CSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT          540 a          351 c          343 g          717 t
ORIGIN      
        1 catttattgc gattttattc gatttagttg cggcagcagc ttattttctc caattaaatt
       61 atcaaacctc aggaattctg tttattggaa tgatttttca aggtgtgatt acccttttgc
      121 ttcttgtcat gatgctcagc tataaaggaa aaagatatgc ccgtattcaa acaaaagtat
      181 ttgttaaata tgtttccatt cgctatggga tcatcattct ttccttttta gtgaatgcga
      241 tcgtcctctt tttatatgta ttgaattact tagatatcaa tccattgatt ttttctcgat
      301 agataagaca cattcagcgg aacagttcct gcttatcagc agaaaactgt tctgcttttt
      361 catttttcat tgatctattc ttcttttatt ttcctagcat ttcatttgtg atgtatgcaa
      421 taacatgtta tcttttatta tatgaatcgt tgaggagtcc gttacatgat tacgttggtg
      481 agagttttgt tttgggtgcc tgcggtcgca ctggtcgcaa gtattgttta tcttatgaat
      541 tggaacaaag aacggtttta tttagctatt ttgactcttc cggctatcta ctttatgtgg
      601 aaagttttta attacaacta ctttgaaccc gatagtgttt ttatagaaga attatcagga
      661 ctggttttgt ctttattgat cgtcattctt tatttgatac gtttaaataa aaaacattga
      721 gtactattga ttgcatgtgt agctttctat agtcattata actattctac tataatgaaa
      781 ggtgattaaa tgagtatcga agaagctatt ttactggata ctttacgttg tcgtctgaat
      841 ttcttaagaa ctggccgatc tttatctcaa gtagctgttt ctagcgcgtt aagtattcct
      901 ttattcaaat atcgtaagta cgaagaagga gaagaaattc caagcatcat tgaattaatc
      961 tatatcgctg acttttacga tatttccgtt gattacctaa ttggtcgctc agaaacagca
     1021 agcaaactga agtgattttt tccagccttg gtgctggttt tttcatttaa cgtctaaggt
     1081 taacaatcta tatataaagg agacatttct atgcgattat ggcatgaaaa attgatttca
     1141 aagcttcctc gtcaacagct tttaggacaa catcgggaat gctgtgcatt aagaggaaat
     1201 ggctggggaa agaaacatgc tactgtggat tatgtttttc actattctcc ttatagatta
     1261 ttccaatatc atcagcttgt catggatgaa atggaaaaac gtggttacag cccagcacca
     1321 gagtggaaaa atcctatgta tcgtggcaaa tcatgcgaag cattttttga tttacctact
     1381 gtagagcgca acgtgccgat ttatccggaa cacgatgaag tttatctagc agaatgttta
     1441 gcaaatttgg agcaaaaagg aatttatctt acctaatata actagaaaag ccttctatct
     1501 cctcttactt cattaaaaag ctttaaccat cttagatcaa gctaaaatgt cgtaagtttt
     1561 agggttatct tcatcctcac gtgcaaaact aaaagcgtac tctagttttg gttcatcgcc
     1621 aacttcccta gacataaatg aacatctcct gacgtggttt aaaagtaccg catcttcgac
     1681 gtctgtatac gatgcacagc acagattgct tcttttgatc tttcttttga tttttctgtt
     1741 gcttcttcta caccttcttc gattactaca tcgatgctta caattgcttc tgcctaatat
     1801 tcccatgcat cgtcgattta agcctctgta gtcactgtga tcgtttccac attttaactc
     1861 ctctgtcgtg gcttcagttg attttgtgat cagttcagtt gctaagccag agtcagaaca
     1921 acctgccatt gctagcgtta atattgatag t
//
LOCUS       DAZULY010000307         1930 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000307 DAZULY010000000
VERSION     DAZULY010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.313"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..>1930
                     /locus_tag="ACG81A_002718"
     CDS             63..>1930
                     /locus_tag="ACG81A_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFS5016004.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLAN"
BASE COUNT          751 a          264 c          338 g          577 t
ORIGIN      
        1 tcttctgtat gaaaatagag ataataattg tacacttaag ctgtattcag gaggtgaaga
       61 taatgggcat cgaatttagc gaacggcaaa tgaaaatact atactttttg attgaaaatg
      121 agcacacaac ttacaaagaa ttatcagaac aattatatgt atctacgaaa acaattagca
      181 aagatatttt aattttgaaa gaaataatcc aaaaaatgcc agatgtacaa atgttggttc
      241 aacctaaaaa aggcgtttcg cttattggaa aaaaagaaac acttcgaaat ttattatcca
      301 gtcataaaat caatatcgca gactctccgg aggaccgtct aacttatgtg tttgctaaat
      361 tattgaatac taatgattct ctaaagattc aaaatcttgc tgacgaatta tacgtgagca
      421 ggtcgacggt agaaagcaca atgaaagatg ttagaaaaag actaaacaag catgggatgt
      481 ccttagtttc agatcgaaat ggcgtgcgca tcaaagctga tgaaaaaagt aaaagaaggt
      541 tgatgtcaga agttgtgaac tattattggg gtggctttac agcatctaac agtaaaacag
      601 atctcttaga cttgaatatc aatttcattg gtgaagcgaa taatttctta aataaggaca
      661 tattgaaaaa agtagccgaa ttgcttaatc gatacatgaa taagacacaa ttgaaattta
      721 cagattatga atttcaaact ttagctatcc accttgctat tgcgatagaa aggattgaaa
      781 caagcaattt cttatcacgt agtaataatt ctgaagaaga aatctcaaag aatacactta
      841 ttttaatcaa aatgattgaa aaagcatttt caattattgt gccaatagat gaacaagctt
      901 atgttgacat tcacattact tcgattgaaa aaaatgctat gaatcacgac ttagatcaaa
      961 cgaagctttc ctatttagat atgggtgttg agctacaaga gttagtaaga attaaattaa
     1021 atgaatttta tcctgatgaa gaattgatca ataatctagt attacatctc aatgcagcag
     1081 ttaaaagact caaattggga gtaaatatct ataaccctta tacggacaaa atcaaatatt
     1141 cttttaaaag gtcatttatg ataagtgtag atttattaga ggaaatcgaa gagagattct
     1201 gtatacattt taatgaagat gaaatcgctt acgtaacact ccatattcaa tcacttttgg
     1261 atcgttacaa acctgacaaa acgaaagtga tattagtttg tagtagcgga tatggaacat
     1321 caaaattatt agagcaaaga ataaataacg gttttgcaga tatggttgag atagaagatg
     1381 tactaagcat caatgaacta caagatagaa aagtcactga tgaacttgtt ataagcacga
     1441 ttccgattga aaatacaaat tttcctgtta ttgttgttag tcctttgatg ttaggggcag
     1501 atgtcagaaa agtaaaacag tatctacagt taaatgaaca aacagaagat gcttttctaa
     1561 atttattaga taaacgattt tgctttttta cccaccaaat aaaggataat caagtgaacg
     1621 ttttaaatat aattaccaaa aaattggtaa aagaaaatta cgcgaaagaa gggatactag
     1681 agggagcaat caaaagagaa aaactatctt caacagcgat gaatgatttt gctatgccgc
     1741 atatcgaagt agattttata aaaaagccag taatctgtgt ctacgttaat ccagaaggaa
     1801 ttgattggga tgggacactt gttcattctg ttttcttttt tgcacttaat gaaaaagtaa
     1861 aacctgaatt gaataaaatt tatgaaacat tcaatgaaat cgcttcgaat catgagttat
     1921 tagcaaattt
//
LOCUS       DAZULY010000308         1915 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000308 DAZULY010000000
VERSION     DAZULY010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.314"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            63..1529
                     /locus_tag="ACG81A_002719"
     CDS             63..1529
                     /locus_tag="ACG81A_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS5016005.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
BASE COUNT          589 a          328 c          393 g          605 t
ORIGIN      
        1 ttgttaaaaa ttgaatatga ttacatctgg ataacaaatt tttatcataa aggagagaac
       61 gaatgaacaa caaacaatta cgctggttta ctgttgcgtt aattgcattt aacatggttt
      121 ggggcatggg gaatgtagtt aataactatg ctcagcaagg agtatcagtt gtcacctctt
      181 ggctattgat tttggccatt tattttattc catacgcatt gatcgttgga caattaggat
      241 cagccttcaa ggatagtaaa ggaggcgtca gttcatgggt cgaaaatacg acatctaata
      301 aacgactggc atactatgcc gcttggactt actgggtcgt ccatattcct tatttggcac
      361 aaaagcctca agcaattctg attgctggtg gttgggcagt ccaaggaaat ggaaatatcg
      421 taaatacgat gtcagtacaa atggtagcag ttatttcact tattatattt cttgcctttt
      481 tatatttatc aacaaaagga ctttctactt tgaaagtgat tggcggactg gccggaacag
      541 cgatgttcgt catgtcgatc ttatttattc ttttagctgt aacggcaccg ttcatcaatc
      601 caacgatgga atttgccaca ccacatatga atcaagtgaa aacatatatt ccaacatttg
      661 atttcagtta tttcacaacc gtttcgatgc tagtatttgc agttggaggg gcggaaaaaa
      721 tatcccctta tgtcaactct acaagaaatc ctgcaaagga atttcctaaa gggatgatct
      781 tcttagcagc aatggtaggg atttgtgctg tattaggctc agtcgcaatg ggtatgctgt
      841 ttgctagtga taatattcct aaagacttga tggcaaatgg ggcatacagt gcttttcaaa
      901 ttttaggtaa ttactatggt atcggtaact cattgatgat catttacgca ttgacaaaca
      961 tgattggaca aatctccgca ttagcctttt caattgatgc accactaaaa atcttgttag
     1021 cagatgcgga tccagaattt gtaccaaact ggctacgtaa acgtaatcgt aaaggtacat
     1081 tggtgaatgg ttatcttctg acaggtatac tagtaggaat cattattttg ttgccgattt
     1141 ttggtatcaa agatatgaat gaattagtaa aatggttgac gaacttgaac tcagttgtca
     1201 tgccaatgcg ttacctatgg gtgttcttgg catatatgat gttgaataaa gcttataaga
     1261 aattcaattc tgaatataaa tttgttaaaa atccaaaaat cggttttatc tttggtgctt
     1321 ggtgctttgc cttcactgcg tttgcatgta tcctaggaat ggtgccaaaa gtagattata
     1381 ctgctgatcc taaagcatgg tggttccaat tatttacgaa tatcatcaca ccaatcgtct
     1441 tgatcttatt aggtatgatc ttgccagcaa ttgctcgtcg agacaaaaac aaaaaaatca
     1501 ctgaaccaga aacagtggtt cagccttaaa atagaaatac ttgttacaag aggttgtgac
     1561 agaagtggtc agcttcaaga aataagaagg aatttacgaa aattgcttct caaatttttg
     1621 tgaagtttcg gcttgttttt cgaagaagct acttctggaa caccgtgaat cagaggttgt
     1681 gaaaaagctg tcctgcatca agtaataaga acagccaaac aaaaatagcc cctcatattt
     1741 ttcgtttggc tgggcttatt accgcagatg caagctttgg aacaccgttt aaggaagagg
     1801 ttgtaaaatc agcgatttga cctgagtatt agagaaacaa acggaaaaat agctcctcat
     1861 attttgccgc tttttgagct aatgctgaag gtcagctgat tgaacaccat ttatt
//
LOCUS       DAZULY010000309         1911 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000309 DAZULY010000000
VERSION     DAZULY010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.315"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            115..729
                     /locus_tag="ACG81A_002720"
     CDS             115..729
                     /locus_tag="ACG81A_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5016006.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(894..1718)
                     /locus_tag="ACG81A_002721"
     CDS             complement(894..1718)
                     /locus_tag="ACG81A_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016007.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT          634 a          326 c          284 g          667 t
ORIGIN      
        1 ttgattgaca tttatttaca aaaaatattg ccatttaaaa agaaaagtga catatggctt
       61 cctcgtgtat gattagggtg tattttaaat agttgcagaa agtaggtaaa agttttgaga
      121 gttgcataca tacgtgtctc atcgatcgat caaaatgaac aacgccagat tgaagaaatg
      181 aaaaaattcg gtgcagagag aattttcatc gagaaacaat ctggcgcaac gattactcat
      241 cgacctgttt tccaagaagc attagattac gtccgagatc aagatatttt tattgtggaa
      301 gctattgatc gacttggaag aaattatgac gagatcatcg cttcagtcaa ttacttgaag
      361 aagaaaaatg tgaagctgat cattactagt ttaccgatca tggctgaagc aattggcaat
      421 cctctcctgg ataaattcat taaggatctg atcatccaaa ttttggccat gatcgctgaa
      481 caagaacgga ctgaatcgaa acgtcgccag gcacaaggaa ttaagatcgc aaaagcaaac
      541 ggcgtctaca aagggcggcc caaattatac agtgcagacg caaaagatcc ccaacgtcgc
      601 cttgtttaca aaagcatcgt tgaagatctt aaaaatggtg ttgcgattgc taagattgct
      661 aaagactaca atgttacgag acaaaccgtg tatagaataa aaaaagacag catggtcaat
      721 gacaaatagt tgatcatgct gcttttatta tcttctcatc tttaatttcc tacatataat
      781 tactatctat aaaattaagt cttactcaga acagataaag cataaaattc caacaaacca
      841 ttttttactt aactaaataa agagtctgtt aatataatct tcacttattt acgttaatcc
      901 cctactgtat ttgttggttc cttatctata ttttctacaa aatccttatt atcttgtgat
      961 ttagcttcgt ataattcttt gtatgtcgac acattggtta tactaattcc tatagctagt
     1021 tcttgagact ctacttttct ctctagctcc tttgacaaag tgatttgaga tttatttggt
     1081 tccgttggag tcaattctat agattcttca taaaaatctc tatacataaa gctatcatta
     1141 attttataaa aaaaattaaa tgtttttagc actgccatat aacattcaaa acattcttct
     1201 atagttggtt tacttccttg aggagaatga accaatttat tacgtttttc tatgaaattt
     1261 ttaatttcct ttcttatttc tttcggaaac tcaatattaa agtacccttt aaacatgctc
     1321 cctaataatt tgtctacagg gggagattgt aaattctcaa atattagagt taaatcaggt
     1381 ttctcttgtt gataaaattc ctttacccct atttcacagg ctgtaattat cgaaataaac
     1441 ctatctctag tatttgatat atttttactt cttttaagga gtataagaga aaaggggata
     1501 ctttcgtgtc catgcatgta attaactaat tgttgagctt cagaagttat atctctaatt
     1561 atatagttaa cggaattagg aagtgtcttt aactctaatg aaatttctat ccaattacta
     1621 tcttctaatc tgtatatgct cgaagtataa tttgattcgt cagcaagcat cgctacatcc
     1681 caaacactga tcataaaatc ttttaaacgt tcaatcattt cagcaatatt gctaaaatca
     1741 gctaattttt ctttagaata aaaaaaacat ttatatcaga atcaataaat tctatttttt
     1801 ttattactat atcgtcatca ttatcttgac catttatgtt tattgttccc tgagtgggga
     1861 gtacttcttt caaattcaag ttaaatttat attccattac ttctcctcca a
//
LOCUS       DAZULY010000310         1905 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000310 DAZULY010000000
VERSION     DAZULY010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.316"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            192..446
                     /locus_tag="ACG81A_002722"
     CDS             192..446
                     /locus_tag="ACG81A_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5016008.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            447..764
                     /locus_tag="ACG81A_002723"
     CDS             447..764
                     /locus_tag="ACG81A_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS5016009.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(1398..>1905)
                     /locus_tag="ACG81A_002724"
     CDS             complement(1398..>1905)
                     /locus_tag="ACG81A_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFS5016010.1"
                     /translation="RQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITN
                     PGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKGITISVPPRKNMDKSEKLD
                     HSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEG
                     TLRYSLGY"
BASE COUNT          645 a          290 c          361 g          609 t
ORIGIN      
        1 ggatgttaac ggaagtgtat ggtgagtagt tcactcgtat tttgatatcg tttgtatatg
       61 ttttcagcgt tctaagtggc ttattttgtc caataataat atggagtgga atcccttaag
      121 aaacaccgtt ggcaattgtt gttataacat gttataattt attcatcaaa aactaaaagg
      181 agtgtgataa catgggaaaa gaactaaagg ttagaaagat cggcaatagc gtgggagtga
      241 tccttccaag ctctcttgga ttaaaaagcg gagacattat ccaagccaaa caagaagaca
      301 acttattcat attagatact actcaaatag caaaagaaca cgatcgcaaa ttgattgaag
      361 aaagtttcca agactttgag aaaggtttaa cagtttcaga aattgaaatg gtaaaagctt
      421 tcggaaaata cggctggagt gaatagatga aagagtacac gctagtttat tctaaaagtt
      481 ttcagaccag ccttgaaacg ataatagaag aatgggaaaa tgagcttgct ctttctccag
      541 ccagcataaa aagatttgta ggtgcgatcc agaaggcttt agaactaacc aaaaattttc
      601 caactatgca tgaggaagtt tccgaaatct atggaatgga tatcccaaca tatcggatcg
      661 tgatcggcaa aagctatgcc ttattttatc ggatcgacca tgaaaaaaaa gagatacttg
      721 taggacgtat tttccaatct aaacaaatga aactaggctt ttaacaataa ttaggaaaaa
      781 gtcgctcatc ctctacaggt gggtgattaa tgccgctcga tatggttgtt aaccgtcggt
      841 tactcgaaga ttgatatata agacttagta cctttttatt ttgctccatg ttatggccga
      901 aggtctaaag atacaatcat tagtctagag aaaaattaag aaccttagga actacgggga
      961 tcgcttggtg aataagagac actgctatta gtgaaaaaat aagatatcaa gtacgctcta
     1021 ttcccaaaaa tttcgtgact ttactcatga gaggttcaag tagtatattt ttaatcaaaa
     1081 ttgacgtaac ttttaataaa tactacctaa ataaggtagt atttattttt gtgttttaaa
     1141 agaggaagtc caatatatct atgggaaact tcttaaatat tatgttggta catgaaaata
     1201 tacttccctg ctagactatt tacaacattt tactttgtac gtattcttgt tttaaaagga
     1261 atgggataaa atataggtac aataactatt tgatgctaaa tataattaat ggaggagaga
     1321 tagtgaacat acttaatgaa aaaagatatg tatggtttgc tggtaaaaaa tatattaacc
     1381 cgtggtgcta gttaatttta atatcctaaa gaatatctca aagttccttc aaaacgtcgt
     1441 tgtgctcgac ttaatagaac actgtaagcc agtaatatgc tttcaaatct actctctagc
     1501 cctttaacag aacgtgagtt gaaattttga caacctaatt tttctaagga agaaaaaaca
     1561 gtctcaactg tttttctttg ttttccaagt aagctgtggt ctagtttttc tgatttatcc
     1621 atgtttttac gtggcggaac ggatatagta atgcccttaa gggcaagctt ttcatggatt
     1681 ttatggctaa tatatccttt gtcccctagg atattgggaa gattcgcttc ttccgataaa
     1741 gtctctaaca ctttcacgtc atggacacct ggattcgtga ttgagtaagt aatcggaaag
     1801 ccagtcttag taacgatcat atgaatttta agaccataaa aataagattt ttttgttgag
     1861 ttatagccaa ctttggctat ttgattcaat agttttgctt gtcta
//
LOCUS       DAZULY010000311         1901 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000311 DAZULY010000000
VERSION     DAZULY010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.317"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..841
                     /locus_tag="ACG81A_002725"
     CDS             47..841
                     /locus_tag="ACG81A_002725"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5016011.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            854..>1901
                     /locus_tag="ACG81A_002726"
     CDS             854..>1901
                     /locus_tag="ACG81A_002726"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HFS5016012.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRIL"
BASE COUNT          694 a          228 c          380 g          599 t
ORIGIN      
        1 gaaaattata atttgctaaa tcaaaaacat aactaggaga aaaaacatga acagttggat
       61 attttgtatt gattcagatg gttgtgtcat ggatacaatg acttataaac acaatctctt
      121 tttcgggcca attgctgcag agatttttga tgttcatcat agggaaaaat tcctacagga
      181 ttggaataat attaatttat attcagagac tcgtggaata aacagatttt taggtttagt
      241 taaaacatta gaaaaaaatg atgtaccatc gattaatcga ttaaagcact gggtatctgt
      301 aacaaaagaa ttgtctaatc catctttgaa gagggaaata gaatactctc agtcagagga
      361 tttattaaaa gctttaaaat ggtcggaaga agtcaatcga cagattagtc aagctaaagg
      421 attagataaa ccctttttaa gtgctagaga tactataaaa gctctgaaaa aatatgggaa
      481 gataattatt gtaagctcag ctaataagga agctattcaa gaagaatggg aaagacatga
      541 attattatcc ttagttgatg aactgtgttg tcaagataaa ggaaaaaaag aagacattat
      601 tcgaagtgta attgaaaatg gttgtgattt ggataagata ttaatgattg gtgattctcc
      661 tggtgatcta gaagctgcaa ataagaataa agttttcttt tatcctattt tggttaataa
      721 agaaaaagaa tcatgggaaa acttaagaac tgatatttta tttaaatgtt tatcgggaga
      781 gtatatagat attgaacaaa aatatataga tactttttgg aaaaatctta ctaataagta
      841 gaggagaaag aaaatgacta taaaagtaga cttacgaaaa aaaccatatc atttagatga
      901 agatgctatt tcttggattg aagagacgat tgatgctatg actttagaag aaaaaatagg
      961 tcaattattt gttaatatgg gatctagtcg tgataaaaat tacttaagta gtatggtcaa
     1021 tcagtaccat attggagctg tacgatataa tccaggaact gctgatgagg tatatgatca
     1081 aaactatatc ctacaaacag aaagcaaaat tcctttatta attgctgcta atacagaagc
     1141 aggtggtaac ggagcttgta cagatggtac cgaagttggt gtagaagtaa aaattggtgc
     1201 aacaaatgat gcgaaatatg cttatgaatt agggagagtt tctggaattg aagcatcggc
     1261 tattggatgt aactggagtt ttgctcctat tgtagatatt aatagaaatt ggagaaatcc
     1321 tatcatttct agtcgatctt ttggacaaag ccctgataaa gttttagaac ttagtttagc
     1381 ttatatgaaa ggtattcaag agagtggaat tttgcctgca gctaaacatt tccctggtga
     1441 cggtattgac gaacgagatc aacatctatc ttttagtatt aatagtttaa gtacagaaga
     1501 atgggataat tcttttggaa aagtatactc aggcttaata gaagctggat taccttcaat
     1561 tatggcaggt catatccata tgccagctta ttcaaaacat tttaatcttg atctgaaaga
     1621 tgaagattta cttcctgcaa ctttatcaaa agaattaatc actgacttac ttagaggaaa
     1681 actcgggttt aatggattag tagttactga tgctagtcat atgttaggaa tgactggggc
     1741 aatgaaacgg tcagaattat tgcccacttc tattgcggct ggatgtgatc tattcctatt
     1801 ttttaatgat ccagatgaag actttaatta tatgatggaa ggttattaca aaggaattat
     1861 ttcagaagag cgtttacgtg atgcattaag aagaatttta g
//
LOCUS       DAZULY010000312         1880 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000312 DAZULY010000000
VERSION     DAZULY010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.318"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..314)
                     /locus_tag="ACG81A_002727"
     CDS             complement(<1..314)
                     /locus_tag="ACG81A_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HFS5016013.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FS"
     gene            566..1027
                     /gene="mscL"
                     /locus_tag="ACG81A_002728"
     CDS             566..1027
                     /gene="mscL"
                     /locus_tag="ACG81A_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFS5016014.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(1087..1533)
                     /locus_tag="ACG81A_002729"
     CDS             complement(1087..1533)
                     /locus_tag="ACG81A_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5016015.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(1626..1871)
                     /locus_tag="ACG81A_002730"
     CDS             complement(1626..1871)
                     /locus_tag="ACG81A_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFS5016016.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT          541 a          384 c          337 g          618 t
ORIGIN      
        1 tgagaaaaaa ggtttggatc attgacatat tcaccattca caaaactgat ccctgcgttt
       61 agccaatgta aatttccttt ttttctaact gataatccaa agcttgcgcc ataaagctct
      121 gcaagttgcg cacttaactt tgtctgatcc gaataattca aactatttgt ttccaacatg
      181 ctgctgagaa gtgaacgttt cgtgatgact tcttgttgca gacgcgcagt aaaacggata
      241 aaaatccgga tcgttttgta ttgttttgtt ggtaagacat gaagattcac gccttctgcc
      301 aattgataca ccatatttat atgctccttt tcacagttga ttgcccgctt actcttttaa
      361 tgggtcaacc tgcctttttt acttattcct ctgcttgaaa tttataagtt caggcaattc
      421 gccgtttttt ctaaatgatg ttacacttta tagtatagca tagatgtttt ttatcaaaag
      481 aaacagacgg ctaaaagcgt atattgtttt tttatttttt atttttcgat atactaaaga
      541 caaataaaag aagggagggt cattcatgat caaagaattc aaagagttca tcatgcgtgg
      601 agatgtcttg gatcttgctg tcggcgttgt tattggtagt gcgtttacaa gcatcgtaaa
      661 ccaagtggtg caaggcttga tcacaccact aataggttgg tttgtcgcaa tgatcacagg
      721 tacttctgat ttagaaggag cactttcaat tcttgactgg tcgccgacaa aaggcgtcac
      781 atttgcgttt ggtaacgtta ttagtgctat cattacattt ctgatcactg gttttgtctt
      841 attcttagtt gtcaaagcgg caaaccgtgc gaagaattta cgctccaaag aagagcaagt
      901 ggaagtggaa gaaactccta ctgcagaaga ttatcttagt gacatacgcg atcttcttgc
      961 caaacaagtg gcagaagaca atacacatcc agtagagcaa acaaatcttt ccgatacttc
     1021 taaataataa agaatctaaa aaaaaagaaa gccgattttc agtgtcggct ttcttttttt
     1081 agattcttaa cttgctactt gttccttttt catgtcgata tgtggaatat catcttccaa
     1141 atacacatct gagattgcct tgaatccaaa cgactcataa aaagcttgta ggtattcttg
     1201 tgcgccgatc acgattggtt tttctggaaa atttttctgg atcatgttaa tcgtttcttg
     1261 aacaatcttt cttcctaaac catctttacg gttgtcctga tgaacaagga cacgtccaaa
     1321 atggatactc tccgcttctt catatactcg cgtgtaggca aggatcttcc catcagtatc
     1381 ttgaaaaaac gtatgccaag cttctccatc aatgtcatca acttcttgat aaggacactg
     1441 ttgttccaca acaaaaactg cgattcgttc tttgacgatc gcgtaaaact ctgctgttga
     1501 caattcttca aatttcttta cctgatattc cactaagcaa acgccccctg cttttttatt
     1561 cttagcatac cgattcgcac acaaaaaagc aaacgaacct tacgaatctc aggctcgttt
     1621 gctttttaaa ctgcttcagt taaaaattcg atctcaactg tccgagtaag tacatctgaa
     1681 tagctgtaag atactcgttc aaaagaattt tcttcaggat ccaaatcaac aacgaatact
     1741 gaaggatagg tttctgttaa aacgccgcgg cgttctgttt gacgctttct tcctgtctga
     1801 gcaaccaaca tgattgggct gccgatacga ccttctaaat ctttcttgat tgaagctaaa
     1861 gttgttggca tacagttcac
//
LOCUS       DAZULY010000313         1875 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000313 DAZULY010000000
VERSION     DAZULY010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.319"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..334
                     /locus_tag="ACG81A_002731"
                     /pseudo
     CDS             1..334
                     /locus_tag="ACG81A_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324676.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(606..896)
                     /locus_tag="ACG81A_002732"
     CDS             complement(606..896)
                     /locus_tag="ACG81A_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016017.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1104..1796)
                     /locus_tag="ACG81A_002733"
     CDS             complement(1104..1796)
                     /locus_tag="ACG81A_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS5016018.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          506 a          362 c          294 g          713 t
ORIGIN      
        1 ttgatgcaaa agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt
       61 tttcttagtt aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa
      121 gacatacaat catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa
      181 aatccaaatg ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc
      241 ttcaatttac ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca
      301 ggaacgttta aaatcctaca attccactac ctgagactag taatgactag tctcttttta
      361 tggcctctga tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc
      421 tttaagcaat atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa
      481 tacatgtagt tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc
      541 ccatcgtctg catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt
      601 gttttctagt ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca
      661 tattctacca caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt
      721 ctattattat aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag
      781 ttcaaactat gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt
      841 ctggcactaa cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc
      901 ttcacctcaa taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta
      961 tctttttgac tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat
     1021 gaaggacatg tactaaaaaa aagaggcagc agtatagatt actgttgctt ctttactaat
     1081 ttaggtttac ttgttaagaa agattattta cgcaaatgtt cttcaacaat ttgctgagat
     1141 ttatcagctg tatcttccac ttcatctttt gctttttcta acttttgaat atattgtttc
     1201 aagcttgagt tttcagcagt tgcttcgtct aatttctttt gtgtatctgt taactgagtt
     1261 tctacatttg ttttttcttg tgtcagtgtt gcgatttgtt gattcaaacc atttacagta
     1321 ttttgtaatt ctgaaagttt tgcttgtgct tcttgtagtc ttgctgtcca atctgcattc
     1381 agatgattga tttcatcatt tttttggttg atctcatcta gtttgacatt gatttcattt
     1441 tgtttgcttt gtagttgtgc atccttgctg actaattgac tttctaagtt ttgtttttct
     1501 gctgccgatt gatttttttg gttttctaat tcatttactt ttgtttggta ttcttggaca
     1561 tctgcctttg aatcttgcaa tgtctgttct ttttgtgcta gtcgagcggt aagatctgta
     1621 agcgcttgct ctttatctgt caatgtatga tcaagctgtg tcagattctc attgattgta
     1681 tcaatatctg tctctccacc ccacaaaata atttcatttg cgtaccgcgt gcctgtgtag
     1741 ccaattagac atagaaatgc aacgacaatc ccaccagtaa taaatttctt tttcatttgc
     1801 atagctcctt aatccaatac ttttacatgc taaaagtaag tgatctcaaa cacaatgtca
     1861 agcgttttta aatta
//
LOCUS       DAZULY010000314         1868 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000314 DAZULY010000000
VERSION     DAZULY010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.320"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            164..1309
                     /locus_tag="ACG81A_002734"
     CDS             164..1309
                     /locus_tag="ACG81A_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFS5016019.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1446..1790
                     /locus_tag="ACG81A_002735"
     CDS             1446..1790
                     /locus_tag="ACG81A_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFS5016020.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT          651 a          337 c          398 g          482 t
ORIGIN      
        1 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
       61 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
      121 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
      181 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
      241 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
      301 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
      361 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
      421 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
      481 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
      541 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
      601 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
      661 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
      721 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
      781 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
      841 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
      901 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
      961 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     1021 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     1081 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     1141 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttggaagcaa tgtatggaga
     1201 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     1261 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     1321 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     1381 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     1441 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     1501 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     1561 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     1621 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     1681 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     1741 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     1801 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     1861 gcaaatta
//
LOCUS       DAZULY010000315         1823 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000315 DAZULY010000000
VERSION     DAZULY010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.321"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..702
                     /locus_tag="ACG81A_002736"
     CDS             <1..702
                     /locus_tag="ACG81A_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFS5016021.1"
                     /translation="DEESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTG
                     MADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSL
                     GYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGV
                     KRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVK
                     RPISADSVLLEEDVE"
     gene            699..>1823
                     /gene="neuC"
                     /locus_tag="ACG81A_002737"
     CDS             699..>1823
                     /gene="neuC"
                     /locus_tag="ACG81A_002737"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFS5016022.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKW"
BASE COUNT          669 a          236 c          361 g          557 t
ORIGIN      
        1 gatgaagaaa gtgcagactt tctaaaacaa attggtgtac aaggatttaa aattggctca
       61 ggagatttaa caaatttacc ttttcttaga aaaatagata tgtatgggtt gccagtttta
      121 ttatctacag gtatggctga tattcatgag gtcagtgagg ctgtagattg tttcatagat
      181 agtccagtta ctattttaca ttgtacttca aattatccgg ctagttctga agatattaat
      241 ttattggcga ttaacacatt aaaagaaatg ttcgggatac ccgttgggta ctccgatcat
      301 tcattgggat atgatgttgg tatttgtgct gttgcgatgg gtgctaaggt aattgagaaa
      361 cattttacct tggataagga actacctggt ccagatcata aagcttcttt agatcctcag
      421 gaatttgcag attttgtaaa gcatattaga aacactgaaa tatttttagg tgatggtgtt
      481 aagcgagcaa tgccttctga acttagtaca aaagaagtat caagaaaaag cctagtggtc
      541 actgagaatt taaatgtggg agatattctt acagagaaat ctcttacaat aaaacgtcct
      601 ggaaatggaa tagctcctaa gtttttatat caatatattg gcaaaaaagt taaaagacca
      661 ataagtgcag atagtgttct gcttgaggaa gatgttgaat gaaaaaaata cttatagtaa
      721 gtggaacacg agctgattat ggaatatata aacctgttgc tgagaattta ctacaagata
      781 attgggatgt atcatttgtt gtttctggaa tgcatttatc ccataaatat ggatttacta
      841 aagaattgat cttagctgac ggatttagaa ttagtggaga agttcaaagt ttgtttttag
      901 agaacaataa gggaaacatg gctcggtctg tggcattaga gataaatggt ttcacaaata
      961 tatttgaaca aactagccct gattttgtat tattactagg ggatagagga gaaatgttgg
     1021 cagctgcgat agcttgtttg tatcttggaa tcaaaacagc acatattcac ggaggcgaag
     1081 tgtctggtac tgtagatgaa tcaataagac atgccataac gaaaatagca tctgttcatt
     1141 ttgctgctac ggaaaaaagc aaagagcgtt taataaaaat gggggaagat tcttggagag
     1201 tttttcccgt cggagctcct agaatagata ctattttaaa taatagatta cctagtttta
     1261 gagatataaa agaaaaatat catctaaaat ttgaaaagaa aaaatacgtt ttgcaagtat
     1321 ttcatccagt tgtaaccgaa ctagaaaata tagaaaaaca aattttagct ttgatcaatt
     1381 caatggaaca acaatctcaa ccaattattt gcattttacc taatagtgat gctggtagcg
     1441 aaattatcag aaaaatgtac cagaaatatt taaaaacgga tattatttat attacgaatt
     1501 tagagccgga ggaatacctg actatattaa aagagtgcaa atttatgatc ggaaattctt
     1561 cttctgggat aattgaagct gcatcattca aaataccagt aataaattta ggaaccagac
     1621 aaaatggacg agaacaatct aaaaatatta taaatgcaaa tctaaatgaa aatgaaattg
     1681 aaactgcttt gaataaaata caagatcctc agtttataaa aagcttgaaa aatgttaaga
     1741 acatctacgg tgacggcaac agttctagaa gaataacaaa aacattgaat caaatattga
     1801 atgatgggga agatattaag tgg
//
LOCUS       DAZULY010000316         1815 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000316 DAZULY010000000
VERSION     DAZULY010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.322"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            119..1045
                     /gene="cysK"
                     /locus_tag="ACG81A_002738"
     CDS             119..1045
                     /gene="cysK"
                     /locus_tag="ACG81A_002738"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFS5016023.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            1281..1727
                     /locus_tag="ACG81A_002739"
     CDS             1281..1727
                     /locus_tag="ACG81A_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFS5016024.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT          618 a          274 c          402 g          521 t
ORIGIN      
        1 attaccatat atggtatatc tttgtctttt ttttgctagt caaagacgaa tcactcgcca
       61 taaatgaaca gattgtatta tgataagtaa gaagagcaaa gacaaggagg aattgaagat
      121 gacagctatc tataactcag tcaccgaatt gattggcaaa acgccaatcg tcaaattaaa
      181 taaaatcgta ccagaagatt cagcagatgt ttttgtaaag cttgagtttt ttaatccagg
      241 aggaagtgta aaagatcgta ttgctttaag tatgatagaa aaagcagaac acgatggtct
      301 actgaaacca ggtgatacta tcattgaacc tacttctgga aatacaggaa tcggattgtc
      361 gatggtagga gttgcaaaag gatataaagt aatcatcgtg atgcctgaga cgatgagtat
      421 cgaaagacgt ttattgatga aagggtacgg cgcagagttg attttgactc ctggtgcgga
      481 tggtatttct ggatcaatca gagaagcaga acgtttggca aaagaaaacg gctacttttt
      541 accattacaa tttgaaaatg aagcaaatcc tcttgtgcat gagaagacga ctggtcctga
      601 gatacatcaa gcatttggag taaatggctt ggacgcgttt gttgcaggta tcggtacagg
      661 aggaacgatc actggcgctg gccgagaatt aaaacgtgtg tatccgaaaa tcgaattaat
      721 cggggtagaa ccagcagaat ctgctatttt agaagggaaa gaagcgggac ctcacaaaat
      781 ccaaggaatc ggtacaggtt ttgttcctaa aacactggat acttctgtgt atgataaagt
      841 cctttctatt agtggagacg aagcgatgga aacagctaga gaagttgggc gaaaagaagg
      901 gattttagta ggcatctcat ctggcgctgc aattgctgcc gcgctaaaag tcgcaaaaga
      961 attaggaaaa ggcaagaagg tattagctgt ggttcccgac aatggtgaac gttatttatc
     1021 tacagccctt taccaagaga tataaggcac ttggtttttc tccagatagg tttatgtaaa
     1081 aaacttttaa agatgaaaag aacacacaaa aatttatgat gagaaaaatt atttgaatag
     1141 agacgtagaa tagtgaacgc gactgtttta cgtctttttt cattaaaata caataaaatt
     1201 gaattttctt ttcatgttaa gtggatttta aaatgtaaaa atagtatgat aagacgagaa
     1261 taacaacgtg gggtgtttac atggaaaata agttagtcga agaggttatc gaaaagttaa
     1321 aaaaagagaa cattcgaatc acacctcaac ggtacgcggt tttagaatat ttgattgaac
     1381 atcattccca tccaacggct gatgagattt ataaagcact tgaacatcgg tttccaaata
     1441 tgagtgttgc gactgtttac aataaccttc gcttatttac ttcgattgga tttgttcaag
     1501 aaatgaagta cggcgatgct tctagccgat ttgatttcag ttcaaagcgt cattatcatg
     1561 taatttgtga aaactgcggt aagattgttg attttcatta cccagggctt gaagatatag
     1621 aaatggctgc aagcagactg actgattttg agattaatga acatcggttg gaattgtatg
     1681 gactctgtcc ggattgtaaa gagtcagcaa aaaaggcgca aatgtaaaaa cagtttctac
     1741 aagtgttata cctaaaaaaa gctagctgtg taaaaaaaac gctagctttt ttattttttt
     1801 gtcttttatt cacaa
//
LOCUS       DAZULY010000317         1808 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000317 DAZULY010000000
VERSION     DAZULY010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.323"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1536)
                     /locus_tag="ACG81A_002740"
     CDS             complement(<1..1536)
                     /locus_tag="ACG81A_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5016025.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQ"
     gene            complement(1600..1808)
                     /locus_tag="ACG81A_002741"
                     /pseudo
     CDS             complement(1600..1808)
                     /locus_tag="ACG81A_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
BASE COUNT          561 a          336 c          246 g          665 t
ORIGIN      
        1 ttgaattttt gttaaataaa tatcccctat ttgaataaaa cgattgacta attgaacttc
       61 tcgactacac caactattaa tatcattgat gaaggaatat aatttatcgt ccgatgttaa
      121 ttggatttga taaatccaag aattcaaaaa gatagacgtt atttgaagat aataatagtc
      181 aaattctatt tgaaaatctc tctctatttc cttaacatac tgtctaaatc gaatctcttg
      241 ataggtggga tatgttaaag ctgaattatt ttggatatca acattctttt gtctaaaata
      301 tctatcatat ttctcaaaaa gctgctgctt atcagtgtct ttcttccacg tttgataaag
      361 atactctata agttcgttga tattaaaaac ttcgtctact ttttccaaat catccatttg
      421 aatatatttt aattcattct gtcttttcat aaccttcatg ccgttgaaat tcacccattg
      481 tggtaaatga tacatctgaa tataaagttc ttccaaatca tctaagaaat aaaaggcatt
      541 ggcacaggtc aattgaatga tatttttcaa attgccaaca ttttcactaa agcgatagtt
      601 taataaaaaa tctaataatt gttgagatat cagtattttc tttgctagtc tttcttcttc
      661 tgatttaaaa aatatcttga ttaactgttt tctttcataa agagaacgac tgtctaatgt
      721 tggaagtgtt agctttacag gtattcttct caacaaagtt gataatagtt tatcctcagg
      781 tggttctgat gttgcaaata taatccagca acttgatgtg taccagtttt cattatcacc
      841 catccgatga aatttcctcg tatccataaa ctgaaaaagt ttctcctgac attcattgct
      901 caatgcatga atttcatcta aaaataagac tccattatta gctaattctg ctaaaccctt
      961 tcttccatct tcggctcctg tatatgcacc ttttacatgc ccaaaaaaat tggacaagaa
     1021 aagttctgga ttattggcat attcactaca attaactgaa atgaaagatg ccttctcttc
     1081 tatataatta ttggccaaca aatccttata cacataattt gctaaatagc tttttccagt
     1141 tccagtctcg ccatgaagta aaatgggtaa gccatttggc ggatagtaga ttgctgcttt
     1201 aatttgagca attacatttc gcagacttcc ttgacttccg ataacccttt ctattccaac
     1261 cctaaaagag ctatcatacc atttttccgc ttcctctgaa gagctaaaaa tttgttgatt
     1321 tggaactttc tccggtaaag aaaagaagat tactggacga gtattgattt ttccaaattc
     1381 tccatctttt acgaattcat tcaaatattg agagacaata ttacgactaa catttaattt
     1441 ttcagatatt ttctgtgctg tattgttccc ccatttattt ttaatctcag agttctctat
     1501 gacataattg acaatgcgtt cttttaattc ttccatcaca gcacctcctc gacactattc
     1561 gaattttgcc gacagtattt ttccataaat atgacttgct aaaaaggctt tgtttagatc
     1621 attcaattgg aacgttgctt gttttgcttg atccgctaag acgttgaaaa gatactctgg
     1681 cgaaatcgca taccccaaaa tatcaacgat tgttgcaatc agatcttctt ttgattcttc
     1741 atctgaaagt aattcacgat acaacatcgt ttgtttgctt actgtgtcat attcctctaa
     1801 ggattcat
//
LOCUS       DAZULY010000318         1804 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000318 DAZULY010000000
VERSION     DAZULY010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.324"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..614
                     /locus_tag="ACG81A_002742"
     CDS             48..614
                     /locus_tag="ACG81A_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS5016026.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            759..846
                     /locus_tag="ACG81A_002743"
     tRNA            759..846
                     /locus_tag="ACG81A_002743"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:791..793,aa:Ser,seq:cga)
     gene            1180..1746
                     /locus_tag="ACG81A_002744"
                     /pseudo
     CDS             1180..1746
                     /locus_tag="ACG81A_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
BASE COUNT          625 a          277 c          352 g          550 t
ORIGIN      
        1 gtacaataaa acaacatata ctgcagtaaa gaagtggggg agttttaatg agaagaaaag
       61 tctataccaa ggatcaaatt ctaaaagctg cttatgatgt tgttgcaaag gaagggttta
      121 aaggatttac tgcgagaaat attgctaaaa agatggggat ttcgacacag ccgatttatc
      181 tggaattcaa aaacatggaa gatttaaaaa atacgttgct ggattctatc tttgaacaac
      241 taaaagaaga tgttttccct attgaacgta ctggtgataa aatcattgat ctcggtttga
      301 actatatcta ctttgcaaaa aagcatcata gtctttacat ggcattgtac attgatgaac
      361 atggggacgg aaagaaaatg catgattttt catacaatta tttccgtgaa atagttaaag
      421 aagatgaaag atacaatcaa ttatcggatg aatatgtcga tgctctgcat actggtacat
      481 gggtagcagt gactggctta gcgtcattga tgtcatcaaa catcatgcat ccaacagaag
      541 aacaaatcat tgcactgatc aaacggacaa ttgactcgat tttagcatta gataatcctg
      601 aaataatcag atagtgttct atcagtatga atttgatcgg taagtccgtt ggctgcaaac
      661 tgtcaatgga cttttttgct aaaaaaagcg atttctgtta tttatctatt agaaataggc
      721 ttgacatttc gtgaaaaaag tttaaaaata gtacttacgg aaggttggca gagtggtaat
      781 gcaccggact cgaaatccgg cgaaccggct aataccggcg cgcaggttca aatcctgtac
      841 cttccttata tcaacgatta gaggttgtga agaagctgtc ctgcatcaag taataagaac
      901 ggccaaacaa aaatagcttt tcctattttt tcgtttgatt gggcttatta ccgcagatgc
      961 aagcttttga acaccgtttg ttcggaataa ggggctgtga caagactttt gtcacagccc
     1021 cttattgttg tacttttaac ctacaatatt ttttgattcc ccctgagtac tattttttat
     1081 aaatgctagt aaatcaatct tctttggcct ttttttgata aaaaaaatac tagatgtaag
     1141 ataaaagtgt aaaaacgatt tgttttagga ggggaaacga tgtgtaccgc aatcacttat
     1201 gcaacgaaag accattattt tggaaggaat tttgattatg agatgtctta taatgaagtg
     1261 gtcactatta ctccaagaaa ttatcgtttc gattttagaa aggtaaaaaa tttagataaa
     1321 cattatgcaa tgataggaat agcagctggg gtatccaact atccactcta ttatgaggct
     1381 acaaatgaaa aagggttgag tatggcagga ctgaattttc ctggcaatgc tgattataaa
     1441 gaattacaag aaggaaaaga taacgttgca ccatttgagt ttattccatg gatactaggg
     1501 caatgctcaa ccatagacga agctaaagaa ttattggcta ctatcaatct agtcaatatc
     1561 gattttagtg aaaaactacc cttgtcacct ttacactggt tattagctga taaagaaaaa
     1621 tcgatagtaa ttgaaagtat gaaagatgga cttcatttat atgataatcc tgtcggtgtg
     1681 ttgacaaata atccgccatt tgattatcaa ttatttaatc tgaacaatta tcgctcgcta
     1741 tcaaatggaa cgagagtata aaatattttg tgtaaatgaa aaaatccata caaaaaagga
     1801 agtc
//
LOCUS       DAZULY010000319         1799 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000319 DAZULY010000000
VERSION     DAZULY010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.325"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="ACG81A_002745"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="ACG81A_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFS5016027.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          506 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaaaaa
//
LOCUS       DAZULY010000320         1728 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000320 DAZULY010000000
VERSION     DAZULY010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.326"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..423
                     /locus_tag="ACG81A_002746"
     CDS             <1..423
                     /locus_tag="ACG81A_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HFS5016028.1"
                     /translation="KKIIKKLNMPSEQYGIAVHPSAIVAKEISIGYGTVIFANAVINP
                     DAVIGEHAIINTGSIVEHDCRINNYVHLSPGVCLAGGVHVGVGTQIGIGSQCIQMKKI
                     GSWCMIGAGSTIVKDIPSHSLAYGNPAKIKKEGINFEF"
     gene            374..1555
                     /locus_tag="ACG81A_002747"
     CDS             374..1555
                     /locus_tag="ACG81A_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFS5016029.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            1568..>1728
                     /locus_tag="ACG81A_002748"
     CDS             1568..>1728
                     /locus_tag="ACG81A_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5016030.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLT"
BASE COUNT          604 a          235 c          364 g          525 t
ORIGIN      
        1 aaaaagatta taaaaaaatt gaatatgcca agtgagcaat atggaatagc tgttcatcca
       61 agtgctatag tggcaaaaga aatttcaatt ggttatggaa ctgttatttt tgcgaatgct
      121 gtaattaacc cagatgctgt aattggagag catgctataa taaacacagg tagcattgtt
      181 gagcatgatt gtagaattaa taactatgtt catttatctc caggtgtatg cttagcgggt
      241 ggagtacatg taggtgtagg aactcaaata ggaattggtt ctcaatgtat ccagatgaag
      301 aaaattggat cttggtgtat gataggcgca gggagcacta ttgttaagga tattccctca
      361 cattccttag cttatggcaa tccagctaaa attaagaaag aaggaattaa ttttgaattc
      421 tagaatttta ttatcaagtc ctcatatggg aggaacagaa gaaaaatatg ttgctaaggc
      481 ttttaaagat aattggatag cgccattagg tgaaaatgtg gataaatttg aagaaagtat
      541 tacaaaatat actggttcta agggcggagc ttctgcgtta agttcaggta cagctgccat
      601 acatcttgcc ttaagactgc ttaatgttgg aaaaaacgat attgtctttg ctccttcatt
      661 tacttttata gctacagtaa atcctatttt atatcaaggg gctgtaccag tgttcattga
      721 ctcagaacct aatacatgga atatgagccc tgttgcacta gagagcgcat tagaagaagc
      781 tgaaagaaac aaccaattac ctaaggcaat catcgtagtg aatttatatg gtcaaatggc
      841 tgattatgaa cgaattttag ctattgcaaa ccaatttaat gttccagtga tagaagatgc
      901 agcggaatcg ttaggttcta cttataaggg aatttcgtca ggcttatttg ggacaattgg
      961 gatctattca tttaacggta ataagatcat cacaacctca ggtggaggag ctctagtgtc
     1021 tagtgataaa aaatatgttg agcattcaag atttttagca acacaagcaa gagatcaagc
     1081 accgtattat caacatagcg aaataggtta taattaccgt ctaagcaata tttctgccgg
     1141 tatcggacgt ggacaaatgg aagtattgaa tgatagagta gctaaacgaa gagaaatttt
     1201 caatcgatat aaggaagctt tttctggaat tgatagaatt gaaatgatgc cagaagtgga
     1261 tggcatggtg ccaaatcgtt ggttgtctac tttaacgatc aatgattata attctattga
     1321 tcaagtagtt aacgtaataa ataaactaaa tgaaaaaaac attgaaacaa gagctttgtg
     1381 gaaaccacta catttgcaac cagtatttca aggggtaaaa atgtattacc atgataataa
     1441 tcttagtgtg tcagaaaagt tatttaatag cggcttatgc ttaccttctg gaagtaatat
     1501 gacagatagt gaacaagaat ttgttattga tgagtttaaa aaaatattca agtaggtgtg
     1561 atattttatg aacataggaa ttattggttt aggttccatg gggaaaagaa gacttagact
     1621 cattcaagaa cactttaatg atattttact atgtggtgtt gatactaatg aggcgagacg
     1681 aaaggaagtt gaaaaggaat ttggaatacc aacttataat agtctaac
//
LOCUS       DAZULY010000321         1702 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000321 DAZULY010000000
VERSION     DAZULY010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.327"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1017
                     /locus_tag="ACG81A_002749"
     CDS             <1..1017
                     /locus_tag="ACG81A_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFS5016031.1"
                     /translation="KLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFMDNEL
                     TDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTNGSLV
                     TQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEEVDVA
                     FCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLVSDIN
                     YIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGNIKCH
                     SLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILDEYKH
                     DILI"
     gene            1073..1291
                     /locus_tag="ACG81A_002750"
     CDS             1073..1291
                     /locus_tag="ACG81A_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016032.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1362..>1702
                     /locus_tag="ACG81A_002751"
     CDS             1362..>1702
                     /locus_tag="ACG81A_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016033.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNL"
BASE COUNT          647 a          194 c          320 g          541 t
ORIGIN      
        1 aaacttcaga tgggtgaaat tgttagaggt cctagtcaaa tatctattga tgttacatat
       61 aaatgtaatt ttagatgtaa acattgttac aatgcgagtg gtggagacaa ttgttttatg
      121 gataatgagt tgactgatga tgaactgtta gaacttacaa aagatatatg taatttaaag
      181 ccatataata tttgtttctg tggaggagaa actttactga gaaaaaaaat agttttatct
      241 tgtgccactt tattaaaaga ggcaggtatt cctaatattg ctattgttac taatggatca
      301 ttagtaactc aggatgttgc aaaagaatta ttcaaagcag gtattaatcg atttcaagta
      361 agtttagatg gaaaaaatgc tagtagccat gaagcactta gattatatcc aaacagtttt
      421 gaaaaggcaa ttaatgctat caaattattt agagaattgt ccgaagaagt ggatgttgct
      481 ttttgtccta ctagttttaa ttacaatcaa ttacgagaag tatatcaact agtaaatact
      541 cttaaagtga agtctttaag aattcaacca tttatgctta gtggtagggg aatgcaaaat
      601 agggaaatag tgccaactgt ttctcaatat agaaaactag tttctgatat taactatatt
      661 aaaagaaatg aagattatac caagatagat tggggagatc caattgatca tatcatccga
      721 cacagaactt tattgaaaga tttttcgaat tttgtttcta ttaggtcaga cggaggatta
      781 atggcatcac cttacttgcc aattcaagtt ggaaatatta aatgtcacag tctttctaaa
      841 tattggcgag caggacttta taaagttttt tctttagatt tagttcaaga tatagctact
      901 caagtttctt caatacaaga tatgagtaaa gaattaaaaa aaggacatat taaatggctt
      961 aatgaagata tagaaattga tattctcgat gaatacaaac atgatatttt aatttaggtt
     1021 tatcattgga aagtttagat aaacaaagta cttttagaaa ggagggaaaa aaatgatttc
     1081 tgaacctcaa atgaataata tgtatgaaag cccaactata atgggagctg gtgaagctgc
     1141 gccaagagct tgttcagcaa ttatggtgtg tgtgttatta gcatctgcag gagtaggtat
     1201 ccaagcagga gcggtgtata cttatgctgt agttgctaca gtagctgtag ctgcagccgc
     1261 agtttggaaa acagtaggat tagttggtta ggaattaata ggagactaac tgtctcctat
     1321 tgattccaat gattaagcta aggaggaatt ttaagaaaat aatgaaatac aataatttaa
     1381 aaccgaaatt acttaatgag aacatagaat atataagaga agaaaattat ggatatttaa
     1441 ccatagttcc caaaaatgga actgaattga aagataacgt tttcaatcca acagtaagat
     1501 atatattggc taactgtact ggaaaaaaca gtgtaaaaaa aatagcgaat ggtttaaaaa
     1561 agaagtttcc tagtttagaa tatgaaaaat tctatcaaga tgttgtagga actattaaat
     1621 ttttcaaaca ctataatatt attgatatag gagattatag tatggaagaa aagaaaatat
     1681 ataaaattga acaaaattta ga
//
LOCUS       DAZULY010000322         1700 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000322 DAZULY010000000
VERSION     DAZULY010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.328"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1508)
                     /locus_tag="ACG81A_002752"
     CDS             complement(27..1508)
                     /locus_tag="ACG81A_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5016034.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT          524 a          231 c          295 g          650 t
ORIGIN      
        1 aataagagga tgtgacaaga attttgtcac atcctctttg ttttgttctc ataacctttt
       61 ttcatataca gttcatgaac cgcttttatt aacttgccaa ataacactgt attgtgcgtt
      121 gtatccagat agaaagtctc cgtttcatct gaaattttct catgctgatc tgtaatgatc
      181 aaatcagcat gttctgaata tgcggtaatt ttaacagcat ctttgcgaaa taactgctgc
      241 aaatggcttt gaagatgata atttcctgta aactgtttgg ttatctgcaa atggatgagt
      301 aactgctctt tcgtttctgt ttgtagtaag tgttggatca attgactaag gtattgactg
      361 atttggtttc tttccccatt ttttgaaaaa attgtatcga ttttttctct taatttcttt
      421 tcctgtataa aagaactaga atgattggac gtggcttctg gaaagcaaag tgtagaaaaa
      481 ctactgctgg attcacccaa cagttcgaaa aaaacaactg ccaacacaag aaaataaaaa
      541 taaattggta ttttattttc taaatggttg agaggaaact gttgagttaa gtaagtcatc
      601 aattgaccga cttgtcgaca aataaggttt ttgcttgtgg aaaataaaaa accaatctcc
      661 tgtttttgtc gaaaagagat ttgatttgga aaaaaacaat agaaaaaata attaaagtag
      721 gctctctctg tttctgtctc ttcacaaggg cgactcactg aaaaagaagt aaagtattct
      781 gaataatcat gtgtatgctg caataagtcc atcaattcag tgatttcaga agaagttttt
      841 ggaaaatatg cctgttgttt gtttcgtaat atgaataaag ttaacagcaa gtagatattt
      901 ttttcttgtt ttgaaatttt ttctatttgc tcatctgtac tgttcatttt tagtgcattt
      961 aattcaagtg aggaaaatgg ccaatcgtta cctccaaatg tatctgaaag aaaaaagtaa
     1021 agatagagac ggatagataa ctcatttcct tcaagagtac agcaattttt tacagatagg
     1081 atccgtaaat ggaatttttt taaggaatga ttgagctgtt ttgtcagttt ttcgagatag
     1141 gagggcgata tataaagttg ttcaagtaaa tatttttttg gtacagcagt ttgtggataa
     1201 ttgattagta acatcaaata gttaaaacga ttggattctt ttaggtagca taattttaac
     1261 ttagaaaaaa cagctaactt tttagtttga tcggtttctt tggtttggat ctctccttta
     1321 tgattagaat aaataaattc atcaatcaat aaaaattgct tgagtgatac atttattttt
     1381 cggatcagtt ctttgattgt ccgttttgga atatgtaagt cattggataa ttcttgtaaa
     1441 gaacaattat ttttttgaat gaccatttgt agaacaagta actctttttt tcttgcttca
     1501 attagcataa atacctcctt tttattctgt ttttgtatat agttagacaa aaactgtgag
     1561 tggaaagtca tttcagtctg tgttattctc acagagtgtc gccttctttt ttatataacg
     1621 ttatttaatt tttttgttac cttgtttatt ctattgttta tttcctaggg tattttgtca
     1681 agatattctt gtttttaagg
//
LOCUS       DAZULY010000323         1695 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000323 DAZULY010000000
VERSION     DAZULY010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.329"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..145)
                     /locus_tag="ACG81A_002753"
     CDS             complement(<1..145)
                     /locus_tag="ACG81A_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5016035.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNM"
     gene            complement(142..795)
                     /locus_tag="ACG81A_002754"
     CDS             complement(142..795)
                     /locus_tag="ACG81A_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5016036.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1083..>1695
                     /locus_tag="ACG81A_002755"
     CDS             1083..>1695
                     /locus_tag="ACG81A_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFS5016037.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          543 a          309 c          233 g          610 t
ORIGIN      
        1 ccatattatg aatcttttct tttctcgtct ccaaatatgt tttaggaaag ataaaaaaaa
       61 ttaatgaatg aactaatata attattattc caagaacaga aaatatttgt atgaacattt
      121 ttggaaatat ctttaatttt ttcattttct ctccaattta tatcctacat ttcttatagt
      181 agttatacag tctaattgta atttttttct aagttctttt atatatacat ctattactcg
      241 atcataagga gtttcttcgc tatctttcca tacataatca ataatttgca ttcttgttaa
      301 cacttgtcca ttattatcca ataaacattt taatacttct agttcttttg catttacatc
      361 tatttcttca ccatttattt tagctgtata gctattaaaa ttaaccgata gatccttata
      421 ttcaaacttc tctaaatgtc cataattctt cttaattaaa gaatctattc tagcttttaa
      481 gaccggcaat gaaaatggct tttctacata tccgtctact aaattagtaa atgcatcaat
      541 tttatattct tcatcactaa atgcagtcaa gattagaatt ggtaaattgc ttttctttct
      601 aatttctttc aacacttcta aaccatttat aaaaggtatc tgaatgtcta agataaccaa
      661 atttatatcg ctattaaatt ttgacaaggc ttctcttcca tctttagctt gaataacagt
      721 atatccaaat tctgaaagat attcacttat tccctctctt atcatattat catcttcaac
      781 agttaatatt ttcatatata cctccactta agcctataca ctagatatta tacagcatat
      841 tatggctcta taatatctag tgttggtgac accttctgtt gccaatactt tttctttttt
      901 ctaataaatg caaacagaca ccttgaaatc ctttagaata aagggttcta taatattttg
      961 tgttgttgga aaatcgaagg cgattttcta ctaacacttt ttcttcgttt caactcaaaa
     1021 agagactcac ttttggtaaa atttagtcga ccaaaacatc accctaccaa aggagtcgct
     1081 ctatggataa tcatactaga aaattactcg gattaacaga taaacattta ttttttgatg
     1141 aagaatggct gattgaaaaa gaatataaag gcgttcaagc gcagtttatt aaagggagat
     1201 tggatgactc accttcccat tgtgaacact gtggatcgat tcgtattatc cgcaacggtt
     1261 cctatacgac acgtactcag atattgaaag tcaaagaaaa gttgactatt ttagaattaa
     1321 aacgtacccg ttttctatgc tatgattgtg ggcagacctt ttctgcgaaa acggacttag
     1381 ttgatgaaca tcatcaacta acaaaggaac tgaagcaggc gattctcata gaactgtacg
     1441 aaaatcaatc acgtaaacta atcgctaaga agtacttcgt ctcggatggg acggtgacac
     1501 ggatcttgcg tgaagcaacg aagcattacc agccacgtat gaacttcctt ccgacggtgt
     1561 tgtgtatgga tgagttcaaa tcaatgaagt ctgtttctgg ttccatgagt tttatttgtg
     1621 ttgatgggac aacccatcag ctatttacga tccttgaaga tcgcagactt tacaaactaa
     1681 ctcaatactt cctgc
//
LOCUS       DAZULY010000324         1672 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000324 DAZULY010000000
VERSION     DAZULY010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.330"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..1622
                     /locus_tag="ACG81A_002756"
     CDS             75..1622
                     /locus_tag="ACG81A_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFS5016038.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
BASE COUNT          560 a          305 c          343 g          464 t
ORIGIN      
        1 tgaaccccta tttcttgtgc gtttcttgtg gtattctgta acaaaatcag ttcgattcaa
       61 ggtggtgaaa aattttggaa actacagata cgttgcttca gttgcttcaa gaagcccata
      121 aaaccaatca agcacaacaa caaaccattc agaatcttac aactgaaatc caactattga
      181 atgaaaaggt caattattta accaacaaat tatttggacg ctctaaagag actctctttg
      241 aggaaacgaa tgggcaattg aatcttttta gtgatgaaga aatttctgta agtgttccag
      301 aagcagctgc caccattatt ccagtaaaag gacatcaacg agttgtagga acgaaagcag
      361 acaaaatcaa acacttacct ataacagaga aagaacatct ccttcctttg gaagaacaat
      421 tttgtgaaca ttgcggttca cagatgaaag atatcggacg gacaaaagta cgagaagaaa
      481 ttcgtttcca tcaggctatg ttggattgtc tcacccatta tcaacatacc tattgttgta
      541 aaagctgcga aaaagaagga ctttcttctt ttaaaaaagc gatcgttcct aaacctttga
      601 tttctaatag cttaggttcc aatagtttag tggcggaaac gatccggatg aaatttggtc
      661 agaaagttcc tgcctatcgg caagagaact attggaaaca aacacatggg ttggatattt
      721 cgagagataa tatcaccaat tggcatatta aagcagtgca aaacgcgtta gaccctttag
      781 gagaacgatt aagagtctat cttaatcaag aagagattct acacggggat gaaacaagtt
      841 atcgtgtgat tgaaagtgcc aaaacagata cctattactg gcagttttgt acaggaaaag
      901 acagtcagca tcccatcgtt tattatcacc acgacgaaag tcgtgctgga gatgttccga
      961 aaacttttct caaggagttt acagggtatt tacactgtga cggctatagc gggtataatg
     1021 ccgtggaaag tgtacgactt gtctattgtt tcgcgcatgt tcggcggaaa tttttcgaag
     1081 caattccaaa aggaaagaaa aacacggata ttcccgcagc tcaagcagtc aaacaattgg
     1141 ataaatggtt tgtcctagaa aagaaatgga aagacttttc tcctgagaag cgattaagct
     1201 gtcggcaaca agaactgcgt ccattattta ttgcttttta tgagtggatg gcaacgatag
     1261 atcctgttgc aaaatccaag ttggatgcag ccgttcaata tgcgtgtaaa ctacgaagtg
     1321 gttttgaacc tatttttgaa gatggtcgtt tagaactcac aaataatcgt gcggagcgaa
     1381 atatcaaaga gctggtgata ggacggaaaa attggttgca ttccacaagt cttgaaggag
     1441 cacgaacttc aggaatcatt ctgagtgtat ataaaacagc agaacttaac ggattgaatc
     1501 ctgtaaagta tctagagttt ctatttgata agataccaaa tcttcccgta ctttcagcag
     1561 aaactttaga tcaactgttg ccttggaaca aggatgtaca acaacatttt tctcgtaact
     1621 aaaaacgcca aacattcccg attctattct ataggtcggg ttatttgacg ta
//
LOCUS       DAZULY010000325         1665 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000325 DAZULY010000000
VERSION     DAZULY010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.331"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(102..479)
                     /locus_tag="ACG81A_002757"
     CDS             complement(102..479)
                     /locus_tag="ACG81A_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016039.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(779..1660)
                     /gene="galU"
                     /locus_tag="ACG81A_002758"
     CDS             complement(779..1660)
                     /gene="galU"
                     /locus_tag="ACG81A_002758"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS5016040.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          462 a          370 c          245 g          588 t
ORIGIN      
        1 tttatataag tgaaaaaagc ttttttccac caattaaata aatctctcat ctcaatagtt
       61 tatccattat tttatctttt tttgcgtata cctactaaat attaccgtct tcttctcgtt
      121 cttctacttt gttcgagaga atctgtacta gaaaaagtag ttgtatgtct accttttctg
      181 cctatccgag actttctttt catatatggg atataaatga ttagatacag tacagcaaaa
      241 aataaagcaa atacgcaagc ttcccaacta cctaacgcca attcaaccat caaggtagca
      301 accccaacgg atgctgcata ccagataaaa atcagaaacg tttctttttt tctgaccatg
      361 cgaaacatga taaatagact aataagcaat agtggcaaca caaatatcca attgcttcct
      421 tccatcagcc acttgagtat atcaagcagc gactgtgcaa cagcgactaa ttttcccata
      481 gtgaacacac accttttttt ataattaaaa aattcgcctt tttattctac tatgaatctt
      541 ctggcttggc tttcatttct cttatgtaat cattttcttt tatacaactt aaaaaaaaat
      601 taaaccatta gcataatatt ttctgaatct attcacgaaa gtctttttct atctttcgtt
      661 ttccacgact caactgatct agagtcaaaa aactaaaacg acagttgcgc aaatgacgca
      721 caagctgccg ctttaataac aaatagcctt tcattctcag caggccactt atttccagtt
      781 aactttctgt tcttagcttt ttactcaact ggataatata tgcacttaat gaagtcccaa
      841 tttccgaatg ttgaagtcca tattcaatat ttgtcttcat atatcctaac ttatcgccta
      901 cgtcgtatct ttttccttta aactctctcg catagaccgc ctctgtctgg ttaagagtct
      961 gaagtgcatc cgttagctgt atctcgtttc ctgctcctgg ttcttgagtt tctaatatct
     1021 caaatatttt aggagtcagt agataacgtc cgataatggc taaattactt ggtgcttttc
     1081 ccatttcagg tttttctaca aaccctttta ctctgcaacg acccttttcg ctatctgttt
     1141 taggatcaat aattccgtat ttagatgttt cattttctgg tacacgcatg acggcaacag
     1201 cggaagcttg tgtttcttca taatccatca tcaattgttg agtcaatggg atatcatctt
     1261 tcatcaaatc atcccccagc atgacgacaa acggttcatt tcctataaat gcttttgctt
     1321 gaagcacggc atcccccagt cctcttggat gagattgacg gatgaagtga agattgactt
     1381 gagtcgtttc ttcaactaaa gcgagtaatt ctgttttttc cttttcccgc aaattgtttt
     1441 ccaattcgat atttgcatca aagtgatctt caatgggtct tttttctttt cccgttacga
     1501 tcaagatgtc ttcgattccc gaatccaatg cttcttccac aataaactgg atcgttggtt
     1561 tatccacgat tggaagcatt tcttttgcca tggcttttgt tgccggcaaa aaacgtgttc
     1621 ctagcccagc ggcaggaatg actgcttttt tgaccttcac ttgct
//
LOCUS       DAZULY010000326         1659 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000326 DAZULY010000000
VERSION     DAZULY010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.332"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            190..546
                     /locus_tag="ACG81A_002759"
     CDS             190..546
                     /locus_tag="ACG81A_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS5016041.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            556..1455
                     /locus_tag="ACG81A_002760"
     CDS             556..1455
                     /locus_tag="ACG81A_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFS5016042.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          555 a          290 c          343 g          471 t
ORIGIN      
        1 aggttgcttt ttgggtcttt ttccgttcaa gaaactgaac gaagaaaagg gaaagttgtc
       61 tcttttgtga taagagaaat cataataaac gaatgactta ctttccaata aattgagaac
      121 agtcagaacc agacaaatta gttgcatctg atgaataaag tgattattct atgacatgag
      181 gtgatacaga tgaaattaga agtgacaaaa gaagcacaag aagtattgaa gaataaatta
      241 gaagcagacg atcaactttt attagatatc gaaaacggtg acggtccatt tgcagaaagc
      301 caagtcagtt gccaattgga tacggcattt cgattgatca ttgtcaaaaa agatacacaa
      361 acagatctaa gtctttattc tgtagtggct gatacaccag ttggaccaat caaaatcaaa
      421 gacagtgcaa tactttacat ggatgatcca tccacacttg ctttagaacc aacttataat
      481 agtttacaat taaaaggacc aagcggcttg agaaaaggca acttgcaagt tgttcgaaaa
      541 gactagaggg ataaaatggg aaaattggcg atcatcagcg acctacatgt ggacatcaat
      601 aaattcggcg aatcagagct gatgcagctt tggcaagttt tgcaaaagca caagatcact
      661 cggcttcatc tagccggaga tacggctaat aaagtagacc gttgcctagc tgttgtcgaa
      721 tttttcgtca ataaaggatt atctacgaca tttcatttcg gcaatcacga gttggcagat
      781 gtgcccaatg aacagatgat ggaccatttt ccagacccac attttttaaa tgaacggtat
      841 attgctttaa atgagcagac cgttctttta gggatgaatg gctggtacga ctatcagttt
      901 tccgaattga aagattacga ggcaatcgta cgaatgaagc gtttgtattg gtatgatcgg
      961 ttcatccagc gtgggaaaac agatctcgag gtcaatgcag aaatgcttgc agctgcaaaa
     1021 aatttgctag accagctgga agcaaaaaaa cttgaggtga ttttatccac tcatttcgtt
     1081 cctaaacggg catttatcat gtatcaatct gctccttatg agcgttggaa caaattaaat
     1141 gcatttttag gatcagaatc attcggagaa ttacttgacc gttattcaaa tatcaaacaa
     1201 gtagtttttg gccatacgca tcgtcgcttt gaagataaat ccatcggtca cacgatttac
     1261 agctgcagac cttttggtta ttattatgaa tggcagctga cccgagattt tgtcttgaaa
     1321 gaacagttgc tagacagcta tgatcctatg aaattacgtg tcctcttgcg tgcccatcac
     1381 ccagcttttc aggcctatca aacacaacat ttgcaagagg aatttgaaca agcgatgaca
     1441 atcgttccgt attgagacag cattagtaat gtgtaaaaga aaataccttg taaaacataa
     1501 ggataagtaa gagcagtaga tccatttatc gtggatatgc tgcttttttt gcggaaaata
     1561 atcagcgaat gtttatttga tcggatgatt tgtaactcgc agagtactag atgcaagcgt
     1621 gtattctttt tttgaacgat aatcacaaaa aaaaatata
//
LOCUS       DAZULY010000327         1653 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000327 DAZULY010000000
VERSION     DAZULY010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.333"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1408
                     /locus_tag="ACG81A_002761"
     CDS             <1..1408
                     /locus_tag="ACG81A_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFS5016043.1"
                     /translation="TVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNT
                     PGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVT
                     NLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNAD
                     KYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYP
                     DGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTW
                     KEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGK
                     TEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVV
                     VSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLI
                     LLGTLGLLGYKNRKKENE"
     gene            1542..>1653
                     /locus_tag="ACG81A_002762"
     CDS             1542..>1653
                     /locus_tag="ACG81A_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HFS5016044.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSE"
BASE COUNT          656 a          249 c          355 g          393 t
ORIGIN      
        1 aacagtagaa ggcgaaaaag tagaagttgg gggcacagta gatctaacag ataatgttac
       61 taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg gcggtacaat
      121 tgataccaac acaccaggta attacgaagg tgtcattgaa gtaacgtatc cagacggtac
      181 gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctgacg ccgataaata
      241 tgaaccaaca gtagaaggcg aaaaagtaga aattggaggc acagtagatt taacagataa
      301 tgttactaac ttgccagcgt taccagaagg aacaacagta acagatgtta ctcccggcgg
      361 tacaattgat accaacacac caggcaatta tgaaggtgtc attgaagtaa cgtatccaga
      421 cggtacgaaa gatacggtga aagttccagt agaagtgaca gacaatcgtt ctaatgcaga
      481 taaatacaat cctgaaggac aaaaagtgac tacagactta aataaagagc cagatgcatc
      541 tgagggaatt aagaacaaag aagatttacc aaaaggaact acttatgctt ggaaagagaa
      601 agtagatgtt agcacagctg gaaataaaaa aggtactatt gtagtgacat atccagatgg
      661 atcatctgat gaagttgaag tagacgttac agtgacagat actcgttccg atgcagataa
      721 atataatcct gaaggacaaa aagtgactac agacttaaat aaagagccag atgcatctga
      781 gggaattaag aacaaagaag atctaccaaa aggaactact tatacttgga aagaaaaagt
      841 agatgttagt acagctggaa acaaaaaagg tactgtagta gtaacatatc cagatggatc
      901 taaagaagaa gtagaagtta ctatttctgt agtagataaa aaagcaccga ataaacctca
      961 agttgatcct attacagagg gtgaccaaat tgttactggt aaaactgaac caaatgcaga
     1021 ggtgacagtt acattaccag acggaagtca acaccatggg acagctgata aaaatggtaa
     1081 ttttacagtt aaagttccta aattagaggc agggacaaaa gtgatagtaa ctgcaactga
     1141 tgaatctggt aatactagtg aacctactaa tgtagttgtt tcttccaaca aaaaagacag
     1201 tggaaaaaac ggtagtaaag gtagtaagac tgacaatcaa ggtagcaaca gcaatcaaga
     1261 caaaaataga ggcaaaagcc agtcctcaaa agtttttcct aagacgggag agagtgacag
     1321 taatatcttt actattagcg gtggtttaat actgttagga acgttaggat tattagggta
     1381 taaaaatcga aaaaaagaga atgaatagaa aatcaatttc ttaagtcttc tctgagaaga
     1441 ggaagaatct ttttcttcct ctttttgctt ttttatgatg ggaaataagt gttagttaaa
     1501 gatgaatata ctttttatag aaaggaaaag aatgtaatta agtggaaaaa atagtaatta
     1561 ttggttcaaa ccattcaggg ctagccgcag ctcgatttct aaacttaagc gattcaaatt
     1621 atgaagttat tctaatcgat aaaaaatcag aaa
//
LOCUS       DAZULY010000328         1648 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000328 DAZULY010000000
VERSION     DAZULY010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.334"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..422
                     /locus_tag="ACG81A_002763"
     CDS             <1..422
                     /locus_tag="ACG81A_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFS5016045.1"
                     /translation="KKTITAYKEEIQSEQYLERNLAMLKLANHVGKGWFAIELGEKID
                     FLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSASLKTSDTADL
                     NIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            419..>1648
                     /locus_tag="ACG81A_002764"
     CDS             419..>1648
                     /locus_tag="ACG81A_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFS5016046.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILI"
BASE COUNT          634 a          212 c          278 g          524 t
ORIGIN      
        1 ataaaaaaac aataactgct tataaagaag aaatacaaag tgaacaatat ttagaaagaa
       61 atttagcgat gttaaaacta gccaatcatg ttggtaaggg gtggtttgca attgagttag
      121 gtgaaaaaat tgacttttta atcaaaattc ctaagtatat tcttaatgct attgcttttg
      181 catgtcagga aatagtagat ctctcaattt ataaaaaaat tctactccat acaatcgact
      241 tgtacgagga aactacagaa ttgtcagctt tatcagctag tttaaaaaca agtgatacag
      301 ctgatttgaa tatagcaata atttctttat tagagtactt tggtaaagat gaggatgttg
      361 tgtatctaat caatcagata gaaaattaca caaaaatact ttatgctgag gtttcaaaat
      421 gacaaatatc ttcgaaaaaa tcgtaaatga tttaaattta gaacaaaaag atgttgtgga
      481 aagtgaagat aacctttatg taaatgcatg tcctgggagt gggaaaacaa gggtacttac
      541 aaggaaaatt gcttaccaag caattaaaca cgatggatca ctaaaaagaa taattgctat
      601 cacatacact aatagagctg ctgaagaaat taaggaacgc ctaagtttac ttagtataga
      661 tgatgatatt aatatttggg tggggacaat acatcagttt tgtcttgaat ttattattta
      721 tccttttaaa atgaatctct caagagtatc aaaaggattt acaattattg atgattatac
      781 tcaaagagct tacgtcagcc aaattaataa tatgctgaat ttaaatctca aaacgtatga
      841 actgaacaaa attgatttgt cactttctca tgattttaaa attaatgaag ttcagtatac
      901 tcaagtttct gaaatgtatc acactatgtt ggaaaaaaat aaagaaattg attttgatat
      961 gattttacta gtagcgtata aaattttaac tgtaaacagt aacgttgcac gtaatattgc
     1021 tagtaatata agggcgattt atgtggatga atttcaagat actagagaat tacaatacaa
     1081 tgtaatagct aaattacttc aaaacaaccc tcaaattcag tcaatgtttg ttggagatat
     1141 tgaccaagca atttatggat cattagatgg tatagctaaa tcagttgaag aactagaaat
     1201 gctaactagt cacacttttc aatcaaagac gctacatggc tgttacaggt ctaatcagag
     1261 attagtagac ttctactcta attttcagag ttgtccttac gagattgaaa gcagagggaa
     1321 taataaaaac gaccgtggtt ttataagtta tgattgcaaa gttactaagg aggatttacc
     1381 caacataata aaaaatataa taaaggaaaa gattgaaagc ggcattcatg aaaaagatat
     1441 ttgtgtactt gctcctcaac actatcctct gttttcactg ggtgagtatt taaagaaaga
     1501 attaccgtat tgcaattttg atgctataaa tatatcacca attaaggtaa ataatcatag
     1561 tttatttttc aagttagcaa ttttgtactt tactgaatca ggtgaaaagg ttcgaagaag
     1621 aaagctaatt gcaaacgata ttttaatt
//
LOCUS       DAZULY010000329         1626 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000329 DAZULY010000000
VERSION     DAZULY010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.335"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            104..829
                     /locus_tag="ACG81A_002765"
     CDS             104..829
                     /locus_tag="ACG81A_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFS5016047.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            1003..1344
                     /locus_tag="ACG81A_002766"
     CDS             1003..1344
                     /locus_tag="ACG81A_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016048.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            1337..>1626
                     /locus_tag="ACG81A_002767"
     CDS             1337..>1626
                     /locus_tag="ACG81A_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS5016049.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT          629 a          236 c          332 g          429 t
ORIGIN      
        1 tatatatatg gttaaagtcg cagtaagaat gtaattgagc tttttcctca tagaatcacc
       61 tcactttcta acgaaaatta taccaaaaag gagagaagaa ataatgcaag aattagtaat
      121 tttgaaaaat aaagaagctg taactacgag cttacaagtg gcagaaagtt tcaaaaagaa
      181 acataagcat gtactagaag caattgaatc aataaaaaga tcggtcgaaa attcggccaa
      241 tgttgaagat gggtccaatt ttggacagat gtttgtggaa gggaacgagc cagactcata
      301 cggaagaagt cggagagttt atttcatgaa tagagacgga ttttccttgc tagctatggg
      361 attcactgga agtaaagcaa taaatttcaa actaaaattt attgaagctt tcaacgaaat
      421 ggaagatgtt attcggaaga atactgttcc tcaaacaatt gaagacatga tgatctatca
      481 attagaagaa atgaaagatg ttaaaaaaga tgtttccatg cttaaagata ctatgcgaat
      541 tagcggacaa caagagtttg aaattaagca aaaaggaaat atgaaagtta tggaagttct
      601 agggggaaaa gaaagccgag cttatgaaga aatcagcaaa aaagtattct caaaattttg
      661 gtctgaattt aaacgtacct tttcaatccc aagatatggc gagttacctc gtaagagatt
      721 cgatgatgct gtttcattta ttgaaatgtg gttaccagaa actgcgatcc gcatggaaat
      781 cgatcaactg aacagacaac aaagactttt cggtgatgaa aatgaataga gctgaagcgc
      841 taagaatagg gacggtaatt gctaatcgct ggtggagaca caataaacca aacatcctaa
      901 gccaacaaca tatcgaaaag caaaaagcat ggcaacaaat aaaaaagtga ctcagccggc
      961 aagcatagag tcacaaaaca tatctaagga gaattttagc atatgaataa agaactttcc
     1021 actttagatc aatatttgac tgattctgaa tggggcaagt cgaatatcaa ggaaacaaat
     1081 aatcgaaaaa tcagacgaaa tcttttgacg aacgaagaac tatcatgtga tcaagatgac
     1141 ttgggaaatt ttgtgagtat ttgggatcat gtttacctta tccatctatc aaaacattca
     1201 aataaacctg aatacatcta tgtcatcgaa gatggcttga ttgatgcgct agaagagtac
     1261 aacagagata acttgattga tatctcttat tacggatcag gtaagaaata cattgctgaa
     1321 atggaggcag aatttgatga gtgaaatcaa agggacaacg aactttgaaa aactttttag
     1381 tcgtaagtta aataaaattc tcaagaaaaa aggaaatttt gattatttat cttgggctca
     1441 cgcgtgggag attatgaaaa agaatgatcc acaggcaacg gtaactatta atgagtataa
     1501 acactacagg gttgtttctg gaactcatca agactttctt gttgaggaat ataaaccttt
     1561 tcttatggat gaaacaggga cttatgtatc tgtctcagta acggttaaag gacatacgga
     1621 aacaga
//
LOCUS       DAZULY010000330         1616 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000330 DAZULY010000000
VERSION     DAZULY010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.336"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..476
                     /locus_tag="ACG81A_002768"
     CDS             54..476
                     /locus_tag="ACG81A_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016050.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            486..689
                     /locus_tag="ACG81A_002769"
     CDS             486..689
                     /locus_tag="ACG81A_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016051.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            900..1484
                     /locus_tag="ACG81A_002770"
     CDS             900..1484
                     /locus_tag="ACG81A_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5016052.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          569 a          227 c          298 g          522 t
ORIGIN      
        1 ttaagattta ttaagaaaac gttttcttag ttttgatttc gaggtgatag acaatgaaaa
       61 ataagtttat tttatcttta ggtattttaa ctattggaat tagtgtaact ggaactacaa
      121 ttcctacatt tgctgatagt acaaatgaac caactactgt agtagataat aaccaagaat
      181 taactccttt agatagggac ggaatagcca ttggtaaccc aactaacgac ttaactgtta
      241 ctgaaaatta tttaatagtt tctccattac ttgctctagc agcttctaaa acgatacgag
      301 tgacaggaac gatgattttt gataaagttg gttggccttc tagacctcct caatcacgga
      361 attggaaaga aaaaagaaat ggttattggt actatggaaa attgtattta caaagttata
      421 aatcagctgg aaacaattgg attgcgactt attcaggaac cttaactaga aagtagggat
      481 tttttatgcc agaatttgtg aacacaccaa atttttatca agatagcgat attctaagta
      541 aaattaattt accgaaagaa gaatcagctt tacaagttca gcgaacttta caggagacac
      601 aaaaaattca aaaatcaatt tctaatctag aagattctga agaaaaaaga aaaaaaagag
      661 accaaaaaac atatcaaaaa gaaaaataat gattttaaaa aaagagtgtc tagtcgacat
      721 tctttttttc taattgaaag tgttcataaa atcacccatt tctggacact ttcttttttt
      781 gctttatttt tcaaaaaaac aattcatttc atgttcataa acccataaca atatctatgt
      841 tcagttaaac tatatggata aagttatgtt acaataaaat taatgaagga gtgatttttt
      901 tgaaaatagg atacgcccgg gtatctactg gtttacaaaa tttggattta caagaagaca
      961 gcctaaataa atttggttgt gaaaaaattt ttactgatca tatgagtggg gcaaaaagta
     1021 atcgaccagg attagaaatg gccattgatt ttgttcgctc tggcgataca cttgtcgttt
     1081 ggcgcttaga tcgtttaggt agaaatatgg aagatttgat ttcaatcgtc aatcgactga
     1141 atgagcgtgg tgtgagtttt catagtattc aagaaaatat tacgatggac aaatcaagtt
     1201 ctacaggaca gctcatgttt catttgtttg cggcatttgc agaatttgag cgtaatttga
     1261 tcctggaacg atccgcagca ggacgagcag ctgctcgtgc aagaggacgg tttggtggtc
     1321 gtcctgaaaa attgtctgaa caagatttgg aattactgaa gactttagtg gatagcggta
     1381 cacctattaa gactattgcg gaacggtgga acgtctcaag aaccaccatt tatcgttatt
     1441 tggcaaaaat ggaggagaaa aatggaaaga caaattctca ttagtttttg tcatttaatg
     1501 ctgaaaattt ttgccattta tctagaaaag tgacaatagt atgagaattt tttgtaagtg
     1561 tctactgata acttattctc aaatgttgta aggattttat tcatttgtta atatta
//
LOCUS       DAZULY010000331         1604 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000331 DAZULY010000000
VERSION     DAZULY010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.337"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..1099
                     /locus_tag="ACG81A_002771"
     CDS             20..1099
                     /locus_tag="ACG81A_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5016053.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          470 a          395 c          406 g          333 t
ORIGIN      
        1 tagacaagga tgaattcaca tgacgcaaaa ccatgataac acagtttcac gcaagggaaa
       61 acacctaacc tactcagaac gttgccaaat cgccatcctg aaaaaggaaa actattccaa
      121 ccgtcaggtt gcggatgtcc tgggctgcgt gccgcagacc ataaacaacg aaatcaaacg
      181 cgggaccgtt actcagctta accgccagaa tcagaacggg aaggtctacg actattatca
      241 tgacgtctac gacgccgatg ccgggcaggc ggcctacgag aagaaccgct tgaactgcgg
      301 cagacggccc ttatgggccg acacggacgc cttcattgaa tgggcggacg acatgatgct
      361 gttggaaaaa tggtccccgg acgtggtggt gggctttgcc agaaaacacg aattgttcga
      421 cccggccatt atccctggga ccaccaccct ctatcagtgg atcgaccgtg gcatcatgaa
      481 aacgaagaat atggacctgc tagaaaaact gtcccgcaaa ccgaaatcgt cccgtcatgg
      541 caaccgcccg aataaacggg tgctagggaa atccattgaa gagcgcccgg aggcggttgg
      601 tacccggaaa accttcggcc attgggaagt cgacacggtc gtaggaaaca agaccaagtc
      661 ggatgccgtg ctgctgacgt tggtggaacg gcagacacgc ttcgaggtca ttctgaagct
      721 ggccgggaag gatgccgaat cggtcaacca agcggtccga cagctgagag aacgggctgg
      781 tgcgtacttc ggccagctgt tcaagacggt tacctcggac aacggctcgg agttcgcggg
      841 actgcacgat gccttgcagg atgtggcaaa catctacttc agccacccct atgcttcctg
      901 ggagcgcggg acgagcgaaa accaacacaa gctgattcgc cgtttcatcc ccaagggcag
      961 cccaatcggc aacgtgagcg aaaaccaaat cttgcggatt cagcgttgga tgaacgatta
     1021 cccgcgaaag attttgggct atgcaacgcc gcatgacacg ttcgtccagg catttaggca
     1081 ggaacggttg acggcttgac acgtgggccc ttgacaacga gcctccctcg gcatggcttg
     1141 tgtctggaaa gctggccggg gccagccagt cgggagacta ccatgcctca gctcattgtc
     1201 aagggcccac tcgattagaa aaattagacg gttggtggta acgagcaacc gggtcaccat
     1261 gggtggctaa cttaaacttg aaatttacga aaataaaaaa aggcatacag acaataaact
     1321 gtctatacgc ctcacagact ctattctata catctaattt tggctgtcct gcaaaaaata
     1381 tgtgttagcc tccatcgaaa gatcaaactt tggaaaacat cattataact ttgcttggta
     1441 agagctttgc taaaacatga tatgacttgt atacaaaatg tggagtgtat tccaataaat
     1501 aggaaggatt acaaaatttg aaaccaaata agtaaaaaat actgtcactt tgaattaatt
     1561 tgaaatagta aattctcatt aaactattga aatgctgata ccaa
//
LOCUS       DAZULY010000332         1570 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000332 DAZULY010000000
VERSION     DAZULY010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.338"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..162
                     /locus_tag="ACG81A_002772"
     tRNA            90..162
                     /locus_tag="ACG81A_002772"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:123..125,aa:Lys,seq:ctt)
     gene            324..545
                     /locus_tag="ACG81A_002773"
     CDS             324..545
                     /locus_tag="ACG81A_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016054.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(869..1114)
                     /locus_tag="ACG81A_002774"
     CDS             complement(869..1114)
                     /locus_tag="ACG81A_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5016055.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(1108..1509)
                     /locus_tag="ACG81A_002775"
     CDS             complement(1108..1509)
                     /locus_tag="ACG81A_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFS5016056.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT          510 a          262 c          236 g          562 t
ORIGIN      
        1 tcttgaaaat ccataaaatt cactctcaat tctgataata agtattgcac ttttttattt
       61 taggtgttag aataacaaag tattaatttg ggctgttagc tcagttggta gagcagatga
      121 ctcttaatca tcgggtcgcg ggttcgagcc cctcacagcc cattgggtgc caaaccctta
      181 aatacagtgt aaacgttggt gcttagcatt tagaaaacgt atatactgta tttttttgtt
      241 ttcaaaagag actgtagtac cccttttttt cactaaaagc ccactgtagt cattgatatt
      301 tttgataaga aaggaacata attatgcgta atgaaattgc taatataaca ataatccgag
      361 aaactttaca aaaccatact gcaaatgaga tctctaaaca tacaggatta aatttgagta
      421 ctataaaaaa actaaaaagt ggagaacgtt caatagaaaa actaaattta catgatgcta
      481 tttgcctcac tgaatttggt cttaaaaata atagaaaaaa tgttgaaatc aacatatgga
      541 aataattctt aatcagctgc ttttgtcaaa agcacatagg aatttcacct ctctccaagt
      601 ttacggcgag ccttacggaa gtatcattat cccaacttca aatcatcgct ttttattgac
      661 tataaaaatt attttagaat gtttatcgct ccttctttac agtaagaaat catggcgcaa
      721 ctaaaatatg ctccttgaag tgttaacgta ctgatagaat gtatttaaat ttcaatgatc
      781 aataggtttt ctgtttaaaa gaatctacta atcctttaac tattagaaga ttcttttaaa
      841 cagaataagt ggaaagggga ctttttagtt agttaatttt aaattttaag atcgcttcta
      901 tcaatttaat ttctagctgt cttagcatat actcatctac ataggtaata aattgccccg
      961 cctcattaaa tccatttgtc aaacataact tagctatata actatgataa tgtttgagta
     1021 tcatcttgat tgaaattgga tcttcttcta gagcagattg gataatggat agaggaacta
     1081 atggatactg ccgttttttg gggttgtcta ccataagtca taccccattt ttaattgttc
     1141 aactgccttc tttcgttgat accagatacc acttgtactc atattgagga ttttaccgat
     1201 ttcagtatca ttaaaacctg caaaataata aagtaggata acctttcttc ttatttccga
     1261 taaatgatca atggctgccg ataacttttg atctgaaatt aatatttcca ttccaaataa
     1321 gaaatagaca tcttgttcat caaaatgagt ttgggagtac ttaccttcat ttgaattcgt
     1381 caatggttcc tcattatctg ttatgttaat gtttcgtttt cgaatagact tagtttcgtt
     1441 tcgtaatact tttttacagt aggcatcaaa ttgatgttgt atagatttct cattgtaatt
     1501 ttgttgcatg ataaatatgc tccttttttc ccctttccac taatacaaca atcagaaagc
     1561 gccttttttt
//
LOCUS       DAZULY010000333         1553 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000333 DAZULY010000000
VERSION     DAZULY010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.339"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..344)
                     /locus_tag="ACG81A_002776"
     CDS             complement(<1..344)
                     /locus_tag="ACG81A_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5016057.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALECFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTG"
     gene            complement(640..1512)
                     /gene="scrK"
                     /locus_tag="ACG81A_002777"
     CDS             complement(640..1512)
                     /gene="scrK"
                     /locus_tag="ACG81A_002777"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFS5016058.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          418 a          357 c          305 g          473 t
ORIGIN      
        1 cccgtcttaa accggcgata gagggtactt actgaacaag agaaggttcg ctctgcacgg
       61 ccaataatga cgtctggggt ccaaccttca gttgatcttt ttgcaatgta ttctttttcc
      121 tcagcaggaa aaatgatttc ggttctccca caacgtcgct tattttcttt gtattgttca
      181 aagcattcaa gtgctgttcc accacatttg agaaagttat agacattgta aattgtttgg
      241 cgtccacgtt taagctgctt cgcaacgata gcaaccggag tttcttgatg aaaatatgct
      301 tctatcatta caagctcgtt tggtgtaaga tgggtataag tcatttatgt tcactctcct
      361 tgtatgcttt agcgggtatt acgatttgag tgtaacataa atggcttttt tatttgtctc
      421 gcttaattat acaaacggcg tgtaaaaaac tattgcacaa tacaagtggc ctacggctat
      481 cgaaatttcc ataactttcg agcaagaatt tatctcattc aaggcttgat cttccaagta
      541 aaacaaaaac cagttaagca ctctgcctaa ctggttatga ctaaattttc agttgtatct
      601 ttctctacca acactaaatg tcgaagagcc ttttttcgtt tatgaatgta agaccgcttc
      661 tcgagccaat gccaaacaac cgagcgttcc agcgttgtct tctaatgctg gggtcacgat
      721 atatttgctt aactcaggtg tttcgacata cccattgacc aattttgcaa atgcgtcgtg
      781 gacgttttgc agcatatggc gttgtttcat cacaccaccg ccaaaaatga tcacatctgg
      841 agaaaacatc aaggttgtat tgtaggcaca ttgagcaata taggaggctt cgatttccca
      901 ataaggatca tcttctgcaa cctcttgtcc ttttttccct aagcgttttt cgatggcggg
      961 tccagcggcc atcccttcta aacagttgcc atgaaatgga cagctgccag caaagtcatc
     1021 ttttggatga gggacgacga gcatatgccc catttctgga tgactaaacc cttcgataaa
     1081 gcgcccttct tgtaaggcac cgccgccaac acccgtaccg atcgtatagt aaacgacact
     1141 gctcaatcct ttgccactgc cgaagacata ctcaccatag gcagccgcat tgacatctgt
     1201 tgtccaagaa atcgggatcg gaaattcttt tttcatggta ccgatgaaat caaaattttg
     1261 ccatgccaat ttcggggtcg aggtaatgta cccataagtg gcggaatcgc gatggatatc
     1321 gataggccca aatgagccaa tcccaatccc tgctaaatct gcttggtatt gtttgaagaa
     1381 gtcaatgact aaaggcattg tttcctcagg tgtcgttgtc ggaaagctca ctctttcctt
     1441 gatcgtcatt tcttcatcgc caatggcaca cacaaatttt gtgccgcctg cttcaattgc
     1501 accgtatagc atagacattt ctccctttat tttagaatga aatcactaaa taa
//
LOCUS       DAZULY010000334         1533 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000334 DAZULY010000000
VERSION     DAZULY010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.340"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(113..547)
                     /locus_tag="ACG81A_002778"
     CDS             complement(113..547)
                     /locus_tag="ACG81A_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5016059.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(647..1495)
                     /locus_tag="ACG81A_002779"
     CDS             complement(647..1495)
                     /locus_tag="ACG81A_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5016060.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT          539 a          303 c          238 g          453 t
ORIGIN      
        1 aaatggattg gcataagata aataagagaa agcacatgaa acagacgcgc tttctcgaat
       61 agcaaacata tacaaaaaat atcagagaca aaaaataatc tcatcataaa cattacatat
      121 atttctcaaa aagttgtttt ctttcttcaa aaattacttc acgatggacc tgatgaagca
      181 tccataaata accaaaagga tctctgaaca ttgagttagt cacaccaaag tccatcattt
      241 ctgtcatttc ttgaatctct acacagccat ttcgtaatgc tttattatgt gtgtcctcga
      301 tatcagagac gatgatattg aaccagatcg tatttggatg ttcagcagta ggagcagtca
      361 aaccaagaga cgtgttttca tccagcatat ggaatctcgt accatacaag gtaaaaatga
      421 cttcattatt gcctctaggg taatctgttg cctcaatcag ttgtatttca tcaaagattg
      481 tttgatacag ttctagtgct ttcaaactat catttaatac aaaatcgatt tccgttcctt
      541 tatgcataaa gatctcctct ccttttcagt cgaacagtta ctagattctt ttattatacc
      601 agctaaagaa aacgcattca aaaaaatagc ggatttactt accaactcat tcgattggat
      661 gataagtaaa tccgcgtccc attacttctg ctacgctgct gacaatgaca aaggcacttg
      721 gatcgatttc ttctatcaat ggtttcattc gggaaaattc atgatcagtc acaacgatca
      781 ataaaacctc ttttgacttt cgattatacc cgccttttgc ctctagcagg gtaaggccac
      841 gtccaatctc agcacttaag cgaaggcgga tgtcttccaa ataatgttca ctcatgacca
      901 taacagattt ctttcgattg atacctgttt cgatataagt catgacaatt gaagtgattg
      961 caatagataa aatagcataa agaaactctt ctattccaaa aataaacaga ttaagagaaa
     1021 caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa tattttttaa
     1081 aaatgagggg agggatcgtc gtaccgccgc tagaagagtt gtttttatat aaaatcgcaa
     1141 ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa caaccgatct gaagtaatca
     1201 ttgtttctgg aactattgca atgattaaag gaaaaaccaa gcttccgaaa agtactttaa
     1261 aaaagggttt tttacctaaa aatagaagag ctagtaccaa cataacgata ttcaacacta
     1321 aaataactat agctcggtca aagcctaaag cagcttccag taatattcct agaccgctga
     1381 cacctccagc agcaatatga tgaggcccta aaaacatatt gatagcgact cctaatatga
     1441 agagggcaaa aataatctta atgtatgtcc atataaattg tctcgtttct ttcatttatt
     1501 cacttcctta attcattaat aatactctta tct
//
LOCUS       DAZULY010000335         1527 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000335 DAZULY010000000
VERSION     DAZULY010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.341"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..947)
                     /locus_tag="ACG81A_002780"
     CDS             complement(63..947)
                     /locus_tag="ACG81A_002780"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFS5016061.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(1042..1479)
                     /locus_tag="ACG81A_002781"
     CDS             complement(1042..1479)
                     /locus_tag="ACG81A_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016062.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT          475 a          325 c          234 g          493 t
ORIGIN      
        1 cacagtccct tatcccgaac aagcggtgtt ctcccgccaa ctcctcggtc acaacctcca
       61 ttttaacaat aaacctgtaa atgtgagggc aatactttga ttgtcacagg taatttatcc
      121 ccttcatctc catcaacatt cgtcgtcaat tcttcctcat cagctaatga cacaactaat
      181 tgctcacaag taaggtaggc tagatttttc gtagattcat ctactccttt taataggtct
      241 ggcaatgttt taagcgtatc gatcaaagaa gaatctttta aataaaccaa atggagctta
      301 ccatcatcca cttttgcatt tggaagtaat gtttcaaaac cgccgactga gttagttagt
      361 cctatcagca aggtactgct ctcaatttct tccttctttc catcaacttc tacgtgaaac
      421 gagtaggatt gtgtggaagc taactgtttg attcctgaca tgaaataagc tagttttcca
      481 aatttcgtct tcttctccgc atctacatca ttgattgatt cagggataga gccaattgcg
      541 acgacattca taaaatagtc gtcatttatt tttccaatat ccaatggctt gactgattca
      601 atcgaaaaat gattgatcgc ttcctcaggt tctagcggta tccctaatgc tcgagccaag
      661 tcattcaccg tacccaatgg gaaaaatccg aaatttggcc gatgttcttg ttcagcgatt
      721 ccactgattc cttcgttgac tgttccatct ccgcccatga caaacacact atgataccct
      781 tccgtagccg cttcgcgagt aaagtttttt gcatcccctg ctttttttgt atgcaagacg
      841 accacttcat caaacaccga ttccaatttt gctatcgcta attgctcaaa ttcctttgct
      901 tgttcaccgc ctgaactggg gtttaccact aataaaactt ttttcatcac tttctttccc
      961 ttctgattga tttctttaat ataacgatag cataaagaaa aaaattaggc tgtgatgaag
     1021 tactgttcat gaaacattca tttatttttt gacaagataa aacatccata ctatgaaact
     1081 gaggaaaatt aaaacaatga tataggaaat aaaagaaaat gataggcatt gtatacctaa
     1141 taaagtaaaa atcttaggtg gcagataaaa acacaaaaat agcaagagaa tgtaatagcg
     1201 tttcttccac tttttgttat cagaccttat agcgatcaga ggaataccaa atgcataact
     1261 gaaaaactct tgatgtactg ttaatatgtc acggccttga ataataaatg cgacaatccc
     1321 cgctataatt agtgtgccta acaccaaaac ggaaaattga tgaatatatt ttatatacaa
     1381 atgatccatt gtacttcctg cctttttatt tgtgagaaaa acaaatccgt atacgtgaat
     1441 aacttctaga aataaaagaa atttgcaaaa ccactccatt tatctacttc tacgtgcatt
     1501 ttttgatatt gttttcttta tttaaga
//
LOCUS       DAZULY010000336         1480 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000336 DAZULY010000000
VERSION     DAZULY010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.343"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          551 a          169 c          303 g          457 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat
//
LOCUS       DAZULY010000337         1470 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000337 DAZULY010000000
VERSION     DAZULY010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.344"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..795
                     /locus_tag="ACG81A_002782"
     CDS             34..795
                     /locus_tag="ACG81A_002782"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5016063.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(910..1350)
                     /gene="fabZ"
                     /locus_tag="ACG81A_002783"
     CDS             complement(910..1350)
                     /gene="fabZ"
                     /locus_tag="ACG81A_002783"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS5016064.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          498 a          278 c          268 g          426 t
ORIGIN      
        1 gataacaaaa tagtaaattg gaaaggaaca gttatgaaga aaaataaagt tattttaagt
       61 attagtttag cagcagcatt gctgtttgct gctggttgtt ctgcaaacaa cacgacttct
      121 tctagttcgt ctacagaaag ttcaaaaagt acatctactg cggaaagcac gactgccagt
      181 agcgtcgatt tgaacgcatt ggaattacca caattgacga aagatgtcgc cgaaaatgaa
      241 gatctggtcg aaatggtgac gaataaaggc acaatcaaaa tcaaattatt tcccgaacac
      301 gcaccaaaag cggttgaaaa ctttatgaca catgcaaaag acggttacta caacggactg
      361 actttccatc gcgtcatcaa agatttcatg atccaaggtg gcgatcctaa aggcgatggc
      421 actggagggg aaagcatttg gggagaagga tttgaaacag agatcaacaa ccacctttat
      481 aatataagag gtgcgttatc tatggctcgc tcacaagatc ctaacagtaa cggcagccag
      541 ttctttatcg ttcaaaatac agatgatatg catgatggtc ttttaaaaga cgactatcct
      601 caagcaatca tcgacgctta taaaaacggc ggttatcctt cactagatgg ccattatact
      661 gtcttcggcc aagtgattga aggaatggat gtcgtggatg ctattgcaaa tatcgaaaca
      721 gatagttcag acaaaccaaa agaagaagta aaaatcgata aaatcaatat actgcaagaa
      781 gcaaaacaaa aataaaaaaa taacgtctga atcctgcata gatgacaacg tatgctagga
      841 ttcaggcgtc ttctttttac ataaaaagca agtaagcttc cttttgtttt gctatatcag
      901 gttttatagt caagcatttt ggttgacgat gaacgtgaac tcgcactcac agactttttt
      961 atcatctaca tatgctgcag ataacgctgt accgactgat cccttcattt tggtgatttc
     1021 aaaatgacat ttcagcacat ctcctggaac aactttttga cggaatttgg ccttattgat
     1081 cccaccgata taagctcttt ttcctagaaa ctcttctgat tttaggataa gtatcgaacc
     1141 tgcttgtgcc agtgtttcta gaatcaacgc acccggcatc actggattgt caggaaagtg
     1201 accttggaaa aaatcttcat tgattgttac attttttgtc gcaacgatct tttttcctgg
     1261 ttctaaagta tctacataat caatataaca aataggataa cgattaggaa tcaattccat
     1321 tacttcagta gcagataata cgtaagacat aattaaactc cttctttcag gcaattgcca
     1381 ttttcattac tttgactatc aaagtatcat attcctaaat atttagcaat ctttttttaa
     1441 tttaagaaaa aatagtttga caatcaaaac
//
LOCUS       DAZULY010000338         1469 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000338 DAZULY010000000
VERSION     DAZULY010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.345"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG81A_002784"
     CDS             131..505
                     /locus_tag="ACG81A_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFS5016065.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG81A_002785"
     CDS             complement(619..1248)
                     /locus_tag="ACG81A_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFS5016066.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG81A_002786"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG81A_002786"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          494 a          225 c          258 g          492 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaataca
//
LOCUS       DAZULY010000339         1466 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000339 DAZULY010000000
VERSION     DAZULY010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.346"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(141..1433)
                     /locus_tag="ACG81A_002787"
     CDS             complement(141..1433)
                     /locus_tag="ACG81A_002787"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFS5016067.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          398 a          327 c          264 g          477 t
ORIGIN      
        1 ttcggaaaaa gggactgtga cacggttttt gtcacagtcc cttattctga ataaacagtg
       61 ttcaagttat tattatcgta tagataaaca cttcgactcg ctctggtaag aacgtcctga
      121 ttcagtcgtc gtttttatcc ttagatttgc gcccagacgc tttctaagat gtttgtttgt
      181 gttctatcag gtccgacaga aaatgttgag atacgtacac caacaagctc tgagatacga
      241 cgtacatagt tacgtgcgtt ttctggcagt tctgcaagtg ttttgcatcc tgtaatgtct
      301 tctgaccagc ctggcaattc ttcatagatt ggtttgcagc ggttcaactc tttcaagctt
      361 gctggatagt ggtagattag ttcgccatct aattcataag ccgtacaaat ttttactgtt
      421 tccaatccac ttaatacatc gatcgagttt aaagataggt tggtaatacc tgaaactcgt
      481 ttagaatggc gcataactac agaatcaaac cagcctacac gacgcggacg accagttgtc
      541 gtaccgtatt ctcgtcctac ttcgcggatt tgttctcctg tttcatcaaa taattcagtt
      601 ggaaatggtc catcaccgac acgtgacgta taggctttgc agacgccgac aactttattg
      661 atttttgaag gccctacacc gcttccgatt gttaccccac cggctactgg gttagaagaa
      721 gtaacaaaag gataagttcc ctggtcgata tccagcatta caccttgtgc gccttcaaac
      781 aacacgcgtt tgcctgcatc caaagcatca ttcaagataa cggaagtatc tgtgacgtat
      841 tgtttgattt gttgtccgta ttcataatat tcttcaaaaa tatcgtcaaa atcaagtggt
      901 tctgaatcaa acatttttac aaattgacgg tttttgtcat ctagattcaa acgaagacgt
      961 tcttcaaaaa tttctttatc taataagtct gcaatacgga tacctacacg agctgcttta
     1021 tccatatagg caggtccgat tcctttaatc gttgtaccaa tcttattttc gcccttagca
     1081 tcttcttgta attggtccaa tttgatatga taaggcaaga ttacgtgcgc gcggtcagaa
     1141 atacgcaaat tacttgtttc tacacctttt tcatgtaaat aagctaattc tttaaccaat
     1201 gatttaggat tgactacaac gccgtttccg atcacactga tcttgtcttt ataaaaaatc
     1261 ccagaaggaa tcaaatgcag tttataagtc acaccgttaa acttaattgt gtgccctgca
     1321 ttgtcgccac cttggtaccg tgcaatcact tcagcatttt cactgagaaa gtctgtgatt
     1381 tttcctttcc cttcatcgcc ccattgcgta cctactacta ctacagatga catgtgaaca
     1441 cctcgttcta tattatttaa tcctga
//
LOCUS       DAZULY010000340         1463 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000340 DAZULY010000000
VERSION     DAZULY010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.347"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(128..418)
                     /locus_tag="ACG81A_002788"
     CDS             complement(128..418)
                     /locus_tag="ACG81A_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016068.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(536..796)
                     /locus_tag="ACG81A_002789"
     CDS             complement(536..796)
                     /locus_tag="ACG81A_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016069.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(933..1346)
                     /locus_tag="ACG81A_002790"
     CDS             complement(933..1346)
                     /locus_tag="ACG81A_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFS5016070.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          538 a          241 c          216 g          468 t
ORIGIN      
        1 tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata cttcaaaaaa
       61 gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt taatacattt
      121 taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta agagagatca
      181 taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca tcccatattt
      241 cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg atccagcacc
      301 atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag ggaagtaaaa
      361 acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc tcccccacat
      421 tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt gtatgaataa
      481 cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg gaagtttatt
      541 tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat aagccgattt
      601 cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc ttatggataa
      661 aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg aataagtaaa
      721 agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt aagacaatca
      781 aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc gtaaaaacaa
      841 aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa atatataaaa
      901 atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt ttttgtttct
      961 tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt aaaggttcca
     1021 gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga gaccgaatgt
     1081 ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc atgattggcg
     1141 gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc acagccaggc
     1201 atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt tggataactt
     1261 gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc agggaacatg
     1321 tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta ttttttattt
     1381 atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg ttagctttgt
     1441 gaatatttca tctaatacga tac
//
LOCUS       DAZULY010000341         1461 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000341 DAZULY010000000
VERSION     DAZULY010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.348"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..660
                     /locus_tag="ACG81A_002791"
     CDS             <1..660
                     /locus_tag="ACG81A_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HFS5016071.1"
                     /translation="TTSLQVAESFEKKHQHVLRDIDALKKDVSNFGQMFVEGNEPDSY
                     GRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIRKNTVPQTIEDMMI
                     YQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGKESRAYEEISKKVF
                     SKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEIDQLNRQQRLFGDDN
                     E"
     gene            838..1179
                     /locus_tag="ACG81A_002792"
     CDS             838..1179
                     /locus_tag="ACG81A_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016072.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            1172..>1461
                     /locus_tag="ACG81A_002793"
     CDS             1172..>1461
                     /locus_tag="ACG81A_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS5016073.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT          545 a          219 c          308 g          389 t
ORIGIN      
        1 actacgagct tacaagtggc agaaagcttc gagaaaaaac atcaacatgt tttaagagat
       61 attgatgcac taaaaaaaga tgtgtccaat tttggacaga tgtttgtaga aggtaatgaa
      121 ccagattcat atggcagaaa tcgacgagtt ttcttcatta gtagagatgg atttttcttg
      181 ctggctatgg ggtttacagg aaagaaagct atctacttca aacaaaaata cattgaagca
      241 ttcaacgaaa tggaagatgt tattcgcaag aatactgttc ctcaaacaat tgaagatatg
      301 atgatctatc aattagaaga aatgaaagat gttaaaaaag atgtttccat gcttaaagat
      361 actatgcgaa ttagcggaca acaagagttt gaaattaagc aaaaaggaaa tatgaaagtt
      421 atgggagttc tagggggaaa agaaagccga gcttatgaag aaatcagcaa aaaagtattc
      481 tcaaaatttt ggtctgaatt taaacgtacc ttttcaatcc caagatatgg cgagttacct
      541 cgtaagagat tcgatgatgc tgtttcattt attgaaatgt ggttgccaga aactgcgatc
      601 cgtatggaaa ttgatcaact gaacagacaa cagagacttt ttggtgatga caatgaatag
      661 agctgaagcg ctaagaatag ggacggcaat tgctaattgc tggtggaaat actataaacc
      721 aatcatccta agccaacaac atattgacaa gcaaaaagca tggcaacaaa taaaaaagtg
      781 actccgccgg caagcaaaga gtcacaaaga aaacacatca taaggagatt ttagcatatg
      841 gaaaaagaac tttccactct agatcaatat ttgattgatc ctgattgggg caagccgaaa
      901 attgaggaaa caagtggtcg aaaaatcaga cgaaatcttt tgacgaatga agaactagct
      961 tgggatcaag atgatttagg caaccatgta actatttggg atcatgttta tcttatccat
     1021 ctatcgaagc attcgaataa acctgaatat atttacgtca tcgaagatgg cttgattgat
     1081 gcgctagaag agtacgacag agataacttg attgatatct cttattacgg accaggtaag
     1141 aaatacattg ctgaaatgga ggcagaattt gatgagtgaa atcaaaggga caacgaactt
     1201 tgaaaaactt tttagtcgta agttaaataa aattctcaag aaaaaaggaa attttgatta
     1261 tttatcttgg gctcacgcgt gggagattat gaaaaagaat gatccacagg caacggtaac
     1321 tattaatgag tacaaacact acagggttgt ttctggaact catcaagact ttcttgttga
     1381 ggaatataaa ccttttctta tggatgaaac tgggacttat gtatctgtct cagtaacggt
     1441 taaaggacac acggaaaccg a
//
LOCUS       DAZULY010000342         1454 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000342 DAZULY010000000
VERSION     DAZULY010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.349"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..625)
                     /locus_tag="ACG81A_002794"
     CDS             complement(<1..625)
                     /locus_tag="ACG81A_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFS5016074.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="ACG81A_002795"
     CDS             complement(784..1050)
                     /locus_tag="ACG81A_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS5016075.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="ACG81A_002796"
     CDS             complement(1040..1396)
                     /locus_tag="ACG81A_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS5016076.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          406 a          279 c          181 g          588 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg aatt
//
LOCUS       DAZULY010000343         1440 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000343 DAZULY010000000
VERSION     DAZULY010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.350"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..1406
                     /locus_tag="ACG81A_002797"
     CDS             105..1406
                     /locus_tag="ACG81A_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFS5016077.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          474 a          288 c          264 g          414 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DAZULY010000344         1437 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000344 DAZULY010000000
VERSION     DAZULY010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.351"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..1028
                     /locus_tag="ACG81A_002798"
     CDS             3..1028
                     /locus_tag="ACG81A_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFS5016078.1"
                     /translation="MKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVAVSLAI
                     FGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQRPSKVI
                     YDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAKKRGIT
                     ISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINSGELNK
                     DGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYNIQIVD
                     RVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYNLMNSG
                     GNGRVLR"
     gene            1277..>1437
                     /locus_tag="ACG81A_002799"
     CDS             1277..>1437
                     /locus_tag="ACG81A_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5016079.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT          509 a          188 c          306 g          434 t
ORIGIN      
        1 aagtgaaagt tatatcattc ggagaattat tattgagact tgcagcaccc ggttattcaa
       61 agttatttca aagagatagt ttggagacta cattctgcgg aggggaagct aatgtagcgg
      121 tatccttagc aatatttgga tatcaatcag aatttataac aaagcttccg gattcagatg
      181 ttggatatgc ggcagctaat gctttgcgtt attttggagt aaatgttgat aatattatat
      241 ttggagaggg acgcatggga ctgtattatt tagaaaaagg agcagctcag agaccatcta
      301 aagtaattta tgacagaaaa ttttctgcaa tttcgttagc tgatagtgat gaatttaagt
      361 ggaaggagat cctaaacgat gcaaattggt ttcattggac agggataaat ccagcattgt
      421 ctgagaatct ctttgagacg tccttagatg catgtcaggc tgccaaaaaa agaggtataa
      481 caatttcttg tgatttaaat tatcgatcca agctttggac atcagaggaa gcaaaagaaa
      541 agatggaaca gatactacca ttggttgata tttgtatttg taatgaagaa gatgcagaaa
      601 aagtgctagg aattaaagct gagcatacaa atattaatag tggagaatta aataaagatg
      661 gctatgtgca ggtggctagg gagattcaca agaagtttgg atgtaggtat gtagctacaa
      721 ctctgcgtag aagttattcg gcaagtagaa atggttggag tgcaatgtta tacgaagcag
      781 aaaaagataa agcgtatttt tccacagaat acaacataca aattgttgat cgcgttggtg
      841 gtggagacag cttttctgct ggtataatat atgcgttatg tgaaaatatg tccggacaaa
      901 aggctattga atttgcgact gcatctagtt gtttaaaaca tactatagaa ggtgatttta
      961 atcgaactac aattgaggat gtatataatt taatgaatag cggtggaaat ggtcgcgtgt
     1021 tgcgttagtc aacatataaa gataaatcac agtaattttt atggtgatta tctgtcttag
     1081 tagtgattag aataacgtga aaataaattg ttgagcgttt atactgtttt gaaaaataaa
     1141 tttaacatat tgaaaaatac aaatatatac gtatattata aagcgccgat tgtaaaatta
     1201 agctagacaa ctaaaacagt catttgtgct acactcaaaa tagttccgac caaagaatta
     1261 taaggagtga gtgcaaataa cctataccta tcttacaacg gatgaacttg taataataga
     1321 gtcttatttg aaaattaacc aatctgttgc taaaactgcg cattgcttga atcgttcaag
     1381 tcaaacgatc cataaagtat atctgttctt caagcaaaga aaatcagcct tagaata
//
LOCUS       DAZULY010000345         1436 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000345 DAZULY010000000
VERSION     DAZULY010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.352"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1375)
                     /locus_tag="ACG81A_002800"
     CDS             complement(<1..1375)
                     /locus_tag="ACG81A_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS5016080.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQI"
BASE COUNT          389 a          321 c          235 g          491 t
ORIGIN      
        1 gtatttggtt ttcatccagc ctactgtaat tgattctgat tccatttata tcttgcccaa
       61 aaagtagact tggtgcaatt ccaagaccca tatctactac tttttctgct agttcggggt
      121 ctagtttttt cccatcccaa gtaatccagg catagtaacc acctaaaatt gaatgaagct
      181 tgaaaaacga ttgtgaatct ccccaagcgc ttatcagtcg gcttttttct ttcaactttt
      241 ttcttacatc ttgcattttc aaatcaattg cttcattaaa tacattagcc actgtgattt
      301 gtgtaaggat attcatcgaa aactccatca tttcttgcgc ttgagctagt tgcgagataa
      361 aagtctcagg tcctatcacc caaccgattt tcgttgtttt tcctaaaacc ctagataacg
      421 aaccgacgta taacacatta tccggatcaa tcgttcgtat aggaaggact tcttcttggt
      481 ttagaaatga aagatccgta aatacatcgt cttcaataat tggaagccga tactgtcgac
      541 acaattctac tagcttttct cgtcgttttt gactcatcac ttttcctgta gggttttgaa
      601 aattcgggtt gataagcact gctttcaatt tatttttatg gatcgattct ttcagttttt
      661 ctaagtcgat cccttcttca tccatcggta tcccaaataa acgcgttcct gaagctttga
      721 aaagaggtaa tcggtaaaaa aaagaaggtg actctacagc tatcccctct ccagctgaaa
      781 gaatcgtttg taaaatcaaa aagattgctt gctgaccgcc accagtcaca agaagctggt
      841 tgggggaaaa tgccaattca aatttacttt ccaaataccg tttgattttg ttacgtaacg
      901 gagtatagcc taaaagatcc atttcttttt cttccgctaa gatttcttca agagaataag
      961 aaggaaactc aaaattcggc aaaagctcat ctggcatctc gctagcgtat aaatcaagaa
     1021 acgatgtttt ttggatctgc tttgcttctc ttattttttg ctcataccag tccgtctgct
     1081 cataacgagg agagatcatt tgccgccaat cgattcttgg aacagcgaat ttcccccatt
     1141 cagtctgatt aacaaaccat ccgctgccac gtttgctcgt tagtacccca agctccctaa
     1201 gctcttccaa tgcatggaca acggtggaac gattcacacc aaaaagttgc cctagttttc
     1261 gttctgattc aagttgactt ccaggattga tccttccgtt ttcgatttct ctcataatct
     1321 gttccataat ttgacgataa atcggtcctt gttttttctc tactttttcc cacataagct
     1381 tctcttttct attgtctatt ttttcttttg attttaccga atttacgaga aaaaag
//
LOCUS       DAZULY010000346         1417 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000346 DAZULY010000000
VERSION     DAZULY010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.353"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG81A_002801"
     CDS             complement(150..1337)
                     /locus_tag="ACG81A_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFS5016081.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAZULY010000347         1391 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000347 DAZULY010000000
VERSION     DAZULY010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.355"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..1372
                     /locus_tag="ACG81A_002802"
     CDS             join(33..474,474..1372)
                     /locus_tag="ACG81A_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFS5016082.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          579 a          180 c          244 g          388 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agttcaactt
     1381 ttggggttca g
//
LOCUS       DAZULY010000348         1391 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000348 DAZULY010000000
VERSION     DAZULY010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.356"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1361
                     /locus_tag="ACG81A_002803"
                     /note="aph(2'')-Ia"
     CDS             <1..1361
                     /locus_tag="ACG81A_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HFS5016083.1"
                     /translation="DTVRDFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIG
                     YGQIYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERN
                     ANAVILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVK
                     AMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYN
                     FLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIA
                     SFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNAT
                     TVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGT
                     NFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTY
                     KD"
BASE COUNT          587 a          110 c          234 g          460 t
ORIGIN      
        1 tagataccgt aagggatttt attctttatt taaaactttg caacagaacc gaatcattaa
       61 aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt gaatataaca
      121 atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat actgattatc
      181 attatccaaa aactgatgag atagtctatg gtatggatca atttatagga gagccaaatt
      241 attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt ttgaaaaaag
      301 aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga gcaataaggg
      361 cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa ttacacgagg
      421 gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc acaaatgtta
      481 aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat agtattgaaa
      541 taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac atttttaaaa
      601 caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata tataattttt
      661 taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg tatattagtg
      721 atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca ccagaaattt
      781 attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc agttttttaa
      841 gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat aataaacaaa
      901 atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta actgatatag
      961 aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt tttgagggta
     1021 aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat ggcaataata
     1081 gattaactgg aataattgat tttggagatt ctggaattat agatgaatat tgtgatttta
     1141 tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa gatatattaa
     1201 gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata gttgaagaat
     1261 attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa tttatcgaaa
     1321 atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat atatgaaaag
     1381 ctattataaa a
//
LOCUS       DAZULY010000349         1370 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000349 DAZULY010000000
VERSION     DAZULY010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.357"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            68..>1370
                     /locus_tag="ACG81A_002804"
     CDS             68..>1370
                     /locus_tag="ACG81A_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5016084.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNI"
BASE COUNT          487 a          189 c          257 g          437 t
ORIGIN      
        1 ggtgaaaaga tgaactttca tctgcatatc aagtaattgg tatgaggcat tttttgaggt
       61 gtaggatatg aagttggatt accttataga agaggaagat agagaaaaac tgctgctagc
      121 tagatattta gaatttgaga aaaagaatta tgtagtgctg cctaagttat atgaaacatt
      181 aggtttgtct aaatttaaaa caagaaatta tatcatagaa ttaaataaag aatttactga
      241 attcaaaaca aagccagaga tccaaatttc agaagaagca gagatccatg tcgaacgtct
      301 gaatttgacg gatatcaaaa atttaagaag aatctacttt caacgatcaa aaattgctcg
      361 actgttaacg gagatcgtgg attcaaaagt aacgatcgaa aaatttgcag agaatcattt
      421 tttaagcata tcaagagcct atgtaaaaag aagagaatta gtctcttttc ttgaacagca
      481 tcaaataaag ttaaagaaaa acatattagt tggggaagaa ataaacatac ggaacgtatt
      541 atttggggta tatttcgggg tctttaatgg tttttctatg ccatttagag aaagcgtcat
      601 gaatgaagca aagagtatcg tgcaatattt tgaatatctg ttcaatttaa aactgtctgc
      661 aacaaaacga atcaaattca atttgctaac cactattagt ttgttgaggg cctatgggaa
      721 tcaaccaatc ggaaaacgtt tcttttcaga tgaatacttt aagagctcag aaggaatgaa
      781 gatcatcaag gatttatcac atatttcttt tcttcatatc cctaaagagc agatcattga
      841 tgagatttct tatatcatgt tgtttttgat cacggaagaa gaatttgaat tagatggcgc
      901 atttggactt gcggaagctc attttcatga tatccacatg attagtcaaa aaatgtcagt
      961 ggaattgatt gatcaattac cattttcgat tgaaatctct gaagaagaaa gactatatat
     1021 acgaaaagaa cttcgtatgg gtatgatgca tgtcaatcga aaaaattgga tattcgactt
     1081 tgagatgact agctttacgt ctaaaaagca gctacagttt ttcttcgaga cgtatccaat
     1141 attcagtaca gcaatccaaa aggtcataca agagaataaa cgctatttct tgggaaaaga
     1201 tgaacgtttc atgattcgct tattttatga ttatctcttt ttattggtcg gtatttgtcc
     1261 ggtatcatat tttgaagcac cgattcatgt ctgtattgat ttttcgcaag gatctgctta
     1321 taccgattac attatttctc aagtgaaagg atttaaaaat tacaatattg
//
LOCUS       DAZULY010000350         1355 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000350 DAZULY010000000
VERSION     DAZULY010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.359"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACG81A_002805"
     rRNA            <1..1355
                     /locus_tag="ACG81A_002805"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAZULY010000351         1335 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000351 DAZULY010000000
VERSION     DAZULY010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.361"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(197..949)
                     /locus_tag="ACG81A_002806"
     CDS             complement(197..949)
                     /locus_tag="ACG81A_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS5016085.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(964..>1335)
                     /locus_tag="ACG81A_002807"
     CDS             complement(964..>1335)
                     /locus_tag="ACG81A_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFS5016086.1"
                     /translation="NKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDR
                     ELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNP
                     NMFVNYQNDPYQFTTQVILLF"
BASE COUNT          432 a          234 c          166 g          503 t
ORIGIN      
        1 agaaaagaac tattcatttg gccctttttt ggttccagac catagaaata ttcctccagt
       61 aactaatatt gctgttatgc acgtaactaa taacagctat aaaaattggt acaactttaa
      121 taggattcag aaaaatttgc actagtgaag gaatctattt tattagaaaa atgcaatctc
      181 cctaaagaaa aaaccactaa acagtgcttt ctttcaactt ctccttaatt ttttgtgctt
      241 tccctaaagt aaagtttctg gccaactctg aatcaaaata tagtgaacgt tttttaaaat
      301 ctatttcttt aacgttaaag atatttacta aacacgctcg actaattctt gttaacatag
      361 gatatttctt ttcaagctca ctaattcttc cataaaattc gtactgccca tctttagtac
      421 acaatgataa tctatgtggt aattttgaag cttctacaaa ataaatgtca tttatatcaa
      481 aagtaaatgt ttgagaacct atggaaaaaa caaatgcttg attcgtaacc tgttttgttg
      541 catctatcct ttcttgcgct aataacaata actcaacaat ctcgtttcga tactcatcta
      601 gtgtttgatc ttttgttaca aatcctaacg tttcaactct tctttttatt gttacgggta
      661 acatctcatc atgagtagtt gtaaaaatga tttttgcttg aacatcatat tttctgatta
      721 cttctgctaa ttctataccg ttaacttcat gatttaaatc aatatctaag aaatatattc
      781 cttgctttgg tcggaattgt ttgaggtatt ttttaacttc taacggactt tgtgttttca
      841 atactatctt aaatacttta tcgtgaaaaa gaatgaaatt atcaataatt cgttctaact
      901 gatttaattg tataatttga tcttcacata atattattgg ataactcata aatcctcctt
      961 ccctcaaaac aacaaaataa cttgagtagt aaattgatac ggatcgtttt gatagttcac
     1021 aaacatattg ggattctttt tattgatttc ctctatacta gataaaccta acccttgatg
     1081 tccctttttt gttgttattc cttttttcat tagagtatct atagagagta gtatctctct
     1141 acaactattc gatatcgaaa tttctatctg tttatcatct cggtaaatag ctagagacag
     1201 ttctctatct tgactctcta tagatgcttc aattgcatta tcgagaataa ttccaagcac
     1261 cctaatacag tcaaatattg gaacaggaac ttcaataata gtacttggac attcgaattt
     1321 acaacaaatc ttatt
//
LOCUS       DAZULY010000352         1299 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000352 DAZULY010000000
VERSION     DAZULY010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.362"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..286)
                     /locus_tag="ACG81A_002808"
                     /pseudo
     CDS             complement(62..286)
                     /locus_tag="ACG81A_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(840..926)
                     /locus_tag="ACG81A_002809"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ACG81A_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          460 a          217 c          177 g          445 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagt
//
LOCUS       DAZULY010000353         1286 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000353 DAZULY010000000
VERSION     DAZULY010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.364"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..400
                     /locus_tag="ACG81A_002810"
     CDS             2..400
                     /locus_tag="ACG81A_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS5016087.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="ACG81A_002811"
     CDS             384..1079
                     /locus_tag="ACG81A_002811"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFS5016088.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
BASE COUNT          488 a          193 c          261 g          344 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattactt atttctcatt taaacgatgt
     1201 attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt gaataaattt
     1261 cataaaaaca aatgaaaagt gtcgca
//
LOCUS       DAZULY010000354         1276 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000354 DAZULY010000000
VERSION     DAZULY010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.365"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..1194
                     /locus_tag="ACG81A_002812"
     CDS             28..1194
                     /locus_tag="ACG81A_002812"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5016089.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT          460 a          216 c          245 g          355 t
ORIGIN      
        1 agggtagttt ttttaaggag gaaaatcatg ttagagaaaa ctttatacaa tcaattattt
       61 tcacgttctt tctcgctccc tgtcgaagta aattattggg acggtacaac gaaacaatac
      121 ggagatacgg acactcctcc ccaaatcaaa atcaatatcc atgaagaaat cccaatgaaa
      181 gagattacaa gtaacgcatc tttggcacta ggagaagcct atatggatca tcgaatcgag
      241 atcgagggaa gtatccaagc attgatcaac gatgcttatc agcaagcaaa cagcttttta
      301 cgaggaaaag attatctaaa atggctgcca aagaaagaaa aacatacgaa acaaaaaaac
      361 aaagaagata tccatgcgca ttatgactta ggtaacgact tttataaact ttggctagat
      421 ccaactttga cttattcttg tgcttatttc aatacacctg aagatacgtt ggaacaagca
      481 caaatcaata aagtccatca tatcttagac aaactcttta tcaaagaagg cgaaacgttg
      541 cttgatatcg gttgtggctg gggaacattg atgttcactg ctaccaaaga gtataatgtc
      601 aaggcaactg ggatcacact aagtgaagaa caatacgatt tgattaccaa aagaataaaa
      661 gaagaacatt tagaagataa atgccgtgta ttattaatgg attaccgcga actaaaaggc
      721 gaaacgtttg atcatatcac aagtgtaggt atgttcgaac atgtaggttc tgaaaatctt
      781 gaaggttatt tcaaagtggt aaaagattta ttgaaaccaa aaggaacagc tttgatccat
      841 ggaatcagcc gacaacaagg cggtgcgaca aatgcttgga tcaataaata tattttccct
      901 ggcggttatg tccctggtgt tactgaatta gttgataata ttacaaaaaa cgatttgcaa
      961 ctgattgatt tggaaagctt acgtcgtgac taccaattga cattagaaca ttggacaaag
     1021 aattttcatc aagtccaaga tcaagtcacg aaagaaatgg gtgattcctt ctatcgaatg
     1081 tgggatctat atttacaagc ttgtgcagct tcatttgaat caagtaatat cgatgtgatt
     1141 caatatttgc ttgttcaacc tggaaacaat gacatcccaa tgcatcgtgc ataattgagg
     1201 caaaagtact gtttaaagac tatttaaaaa gaagctaagt tttatgatgc tccagaagtg
     1261 aacttttttg tctaac
//
LOCUS       DAZULY010000355         1271 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000355 DAZULY010000000
VERSION     DAZULY010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.366"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..1170)
                     /locus_tag="ACG81A_002813"
     CDS             complement(76..1170)
                     /locus_tag="ACG81A_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFS5016090.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT          375 a          270 c          196 g          430 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa t
//
LOCUS       DAZULY010000356         1257 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000356 DAZULY010000000
VERSION     DAZULY010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.367"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            101..976
                     /locus_tag="ACG81A_002814"
     CDS             101..976
                     /locus_tag="ACG81A_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFS5016091.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1121..>1257)
                     /locus_tag="ACG81A_002815"
     CDS             complement(1121..>1257)
                     /locus_tag="ACG81A_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HFS5016092.1"
                     /translation="SCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKIYPATKEICHDL
                     "
BASE COUNT          444 a          234 c          216 g          363 t
ORIGIN      
        1 tcgagcaaaa gtgattatca aatatgtaaa atcagctact tttttcttaa acgtttcttt
       61 atactaaata taaataagtg tttcatgaaa ggagaactag atgaataatt ggcttgagta
      121 ttttcctgaa aacgttttgg aacgcggtta cagctatcat ctgcacggtt ttgtccgtca
      181 tttgaactat acaagcaagt acttgtccgc aacagtttct ggcacggaag attataaggt
      241 agtgatcact tgggatgaaa agaccaacat gacttgtgat tgtctatatg ccatagaagg
      301 aaaaaaatgc aaacacatgg ctgctgtctt attcgcttac gaagaacgtc caataaaaaa
      361 atccaactac tcactttccg aactctcgtc ccttgtttcc tccgctagtt cctctcttgt
      421 acgggagctt ttaacagaga ttcttataga acatccgcaa tttatcgaac gcttcaaagt
      481 taagatgcca tttcatgcaa taaattattc agataaatta acaactatca tccataaata
      541 cgatcatatt atcaagaaaa acaaaaacag gaaaacagcg aagttcatca tggagatgcg
      601 aaagttcatc caagaagcag ttgaatcgct cattcagcaa aatgcctatc ttcccgcatt
      661 cgaattgatc aatgaagtga ttgctactct cgaaacattc tattgggagc ctgaggatga
      721 acgaaccctt cttctgatag aggactgtta ttatttatgg aaagaattat tagcagaagc
      781 acctcatgca gaaaaaaggc aaatgttctc ttggttcgtc tgccaagtcg atcatactga
      841 cgcctcttat tcaaaacgct actccataaa aatcctgaaa gaagactttc gggaaaaaga
      901 attttctaac caaaagaaaa aaatagataa aaaaactaaa gaaacggtaa agaaagacga
      961 tttcaatgaa aaatagcaag taaacgataa caaccatcct aaatccacaa gaaatacagc
     1021 agaccttgct ttaaaaacac gaatcatgca aaggatgaag taaagaagac ttaacgcgta
     1081 cccaatatta gtcatttgac tattaggtac gcgttttgtt ttataagtca tgacaaattt
     1141 ccttcgttgc tgggtagatt ttccggcatt gttgatagat tttatgatac ttttctacat
     1201 tttgtaagag aggttccact gcatctgtat attggataaa attctgacaa caacttt
//
LOCUS       DAZULY010000357         1246 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000357 DAZULY010000000
VERSION     DAZULY010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.368"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..742
                     /locus_tag="ACG81A_002816"
     CDS             200..742
                     /locus_tag="ACG81A_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016093.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="ACG81A_002817"
     CDS             898..>1246
                     /locus_tag="ACG81A_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFS5016094.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAZULY010000358         1243 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000358 DAZULY010000000
VERSION     DAZULY010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.369"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            341..>1243
                     /locus_tag="ACG81A_002818"
     CDS             341..>1243
                     /locus_tag="ACG81A_002818"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS5016095.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTAS"
BASE COUNT          488 a          192 c          249 g          314 t
ORIGIN      
        1 caacaaaaaa atgtctggaa aatcgaacat aaaagtgtct gatttgaata tcccagcagc
       61 ggaagaagaa aaacaaaatc gataacattt gttctaagaa ttagttacgt taaatacgga
      121 acgcaagata gacttgaaaa acggtaacaa aagaagtagt atattaaggt gtgatgtgct
      181 acttgacatt tattctgctg agcgaacttt ctggattgtg tagccttata tctattcagg
      241 acgtatcgag cagattagta aaggtgttct tgatgatttt catcttgaac acctttgttt
      301 ttagcaattc gcaaaaaacg aaaagtagga gaattgataa atgaaaaaaa gatttttagc
      361 acttgccatc gtactaggca ctggcttatt gtcaggctgt acgaatgccg gtgaaaaaac
      421 agccgttagt tataaaggcg gtacaatcag cgaacaagaa gtaatggata gcttgaaaaa
      481 aatgcaagga gcagattccg ctgtgcaaca gttgatcgtt tatcaagtat ttgaagataa
      541 atatggagat gacgtatcaa ccaaagaaat cgattctcaa tatgatcaaa caaagaaaca
      601 attgggtgat tcatttgatt cacaattgaa atctgcaggt tacacagaac aaacattcaa
      661 agacagtatc aaacaaagtc tagcctttca agaaggtcta aagaaacata tcaaattgac
      721 cgatgaagat ttaaaaacgg cttgggaatc tttccatcca gaagtggaag ctcaaattat
      781 ccaagtagca agtgaagatg atgcaaaaga tgtgaagaaa gctgctgata agggagacga
      841 tttctctaaa ttagccaaag acaagtcaac tgatactacg acaaaagaag atggcggaaa
      901 agtgaaattt gattccacta ctacaacggt tccggcagaa gtcaaagaag cagcctttaa
      961 gttgaaagat ggtcaagttt ctgatgtgat cacttctacc aatgcttcaa cttatactac
     1021 ggaatattat gtggttaaaa tggtgaaaaa ccaaaacaaa ggcaacgaca tggataaata
     1081 caaaaaagaa ttgaaagaaa ttgcaacaga cacaaaatta agtgacagta ccttccagaa
     1141 taaagtaatt ggcgaagtat tgaaagacgc taatgtaaaa atcaaagaca aagactttga
     1201 aaatgttttg agtacattca cttcagattc tagtacagca agt
//
LOCUS       DAZULY010000359         1226 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000359 DAZULY010000000
VERSION     DAZULY010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.370"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..1177
                     /locus_tag="ACG81A_002819"
     CDS             137..1177
                     /locus_tag="ACG81A_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFS5016096.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          493 a          176 c          204 g          353 t
ORIGIN      
        1 ttttatttct gattttaggt agtatttaat taaaaaacag ttttaatgcg tttgcatttt
       61 ttatttatct ttgctatttt cgaaaagagt aggtaatttt acataaataa aacaacgtga
      121 aagtagagga tctacaatga ctgattttaa attttttaag gcggatagag tttacaacga
      181 attattttat caatttccaa aagtctttat tgtttctgac gaatacaaaa aaatgaaaga
      241 ttcaactaag attgcctata tgcttttaaa agcaagatta gagatcgcaa tcagcaaacg
      301 gcaaatcgat gaagaaggta atgtttattt tacttatacg acaaatgaac tatgtagagt
      361 attaaactgc caaaaacaaa aagcgatagc aatcaaaaaa gagttggaat cctttggttt
      421 attattacaa aagcagatgg gatttaacaa acagttaggg aaaaataatc ctaatagact
      481 atatctagca gaattaaaag tctcagaaaa tgatatctac ttactcgaaa aatttgatag
      541 agagaatagg gaaaacgttg ataaatcaga gggtatgaaa atcataccca ccctcgacga
      601 aaaatcagac gctgaatccc ttggggctca agagggtatg aaaatcatac cgtgccaaaa
      661 cgttgataaa tcagagggta tgaaaatcat accagaactt aataataata tattagacac
      721 taatagacac aatatagaca ctgaaaaaga ccgcctacaa gatcaattgt tgttagacaa
      781 ttttgagaca attatgacaa acgacagcat tgctacgttt gtccctgaac gagtattaaa
      841 tttgataaaa acattttctt caagttacag tgaagctcaa aaaaccgtcc agactattca
      901 taatgcaaag aaaaaagctg aaatagaaag tgatatttcg atagtttttg aagaactcga
      961 tagttattat gtcaatgcag aacaagaatt atacacgaca ctgttaaaag cctatcaaaa
     1021 attaaaaacc gaaaaagtcg aaaatatcca gaacctgatt tttgtctatg taaaaaattg
     1081 gtttatcgaa aaaccaatag ctgctaaagt atcaagtgaa aaacgtttga attatgcaag
     1141 ctccccaagc actattacga aagactggtt agagtgaaac tttactgaca aagtgattgt
     1201 tgcttcatgg agtaacatta aaatta
//
LOCUS       DAZULY010000360         1225 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000360 DAZULY010000000
VERSION     DAZULY010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.371"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(165..779)
                     /locus_tag="ACG81A_002820"
     CDS             complement(165..779)
                     /locus_tag="ACG81A_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFS5016097.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          399 a          238 c          213 g          375 t
ORIGIN      
        1 ttaaacaaaa caagcttaaa atcaaagatg atccatctta aaataactaa aacaaaaaaa
       61 gtagtcgttg ttaatcctca ttaataatcg ttttcttttt ttcaaacgct ccatttttat
      121 gtggaacatt atttttttaa ggaactttgt caacatgtgg ataactattt tccacttgtg
      181 tcctggacta gctgttttgc tgtaaaatac aaaacttgct gcagcaaaaa gatcgttgca
      241 agagaggctg ttccgcagat cgctggatct agatttggca aagtcaagac tgcatcatgt
      301 aaattggcgt ggttgtccaa aaccaaatca gcaaattcaa ataatcgcaa accactgcta
      361 tgttgcgatt tgatacttcg tgacaaatca aatccagtaa caatgattaa tggatgacca
      421 ttttctttga tccactgagc tagttcgatg attgccgggt ttcttccttc attcgaaaga
      481 agaagaaaaa tgtcttctga tttgacagaa agctgatcaa taattgcctg tcctgcacct
      541 tctagcgttt catagacacc tccggctgga ttttcaatca atatacttgg tacaagatcc
      601 ggggtttgat cccaaaattc aaatgctaca ccacttagtg tgcggctagc atatatctgc
      661 aatctaccac cttgattaat ttgtttcgcg atccaagtgc cagcttgaag catcagcggc
      721 tcttctatcc ttaatttctc ttccaattgg tgataatagt caaaataaca taaactcatt
      781 tatctctccc gcctagctat acttgacatg aatagaaaca gctgcctaat ctaaggaagt
      841 acataaaaaa acagtagtta gacagtaaca catgtggtct ttgctgtttc aaacacaaaa
      901 aaggcattga acgaatacgc ccaatacctt gacatagcaa ataaaacaac gtgcagacca
      961 tggaaaatat ctacataggg gtagaatttt caccctagat taggtacaat aaattaggtc
     1021 gatgcactgc attgactatg gcaaaatgat cggtcagggt gtatgtgtgt gactggtcat
     1081 tccctgattc ttagcgccaa ctaagggtca gggagccttt ttttattcag ctgtttctat
     1141 ctgaatttat tgtaagtaat ctcttatcca agtgcaagca agaaaataca aaaaaacatt
     1201 ggtatgattt atatatatat aaata
//
LOCUS       DAZULY010000361         1211 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000361 DAZULY010000000
VERSION     DAZULY010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.372"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..301)
                     /locus_tag="ACG81A_002821"
     CDS             complement(<1..301)
                     /locus_tag="ACG81A_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFS5016098.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWR"
     gene            472..>1211
                     /locus_tag="ACG81A_002822"
     CDS             472..>1211
                     /locus_tag="ACG81A_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFS5016099.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQT"
BASE COUNT          450 a          235 c          178 g          348 t
ORIGIN      
        1 aacgccaagt catcttgaaa taacgcggat aaataatttc ttcataataa tatgcttgga
       61 aaacagcaat acaaattccc attagcaaaa cagaaataaa gaaaaacggt aagatagaaa
      121 ttgtccctgt atttctagac aatgcatgca taccgcccca caaaaaagga atcgccaaat
      181 caggggcttt tattttaaaa cgacgattta acgaaaataa ttttacaaga aattggcaac
      241 caaacaaaac gattaccggg taaatatacc aaaacaagat aagtggtgtg aatgtgccca
      301 tgaacgtaaa ctcctttttt atcgttgctt caagtatact gtatatactt tacacaactg
      361 tttcatttta tttaaattta tgcagttata ttctatatct tacaaacaca ttccatgtat
      421 tccaattccc tatccgttat aataaaagaa acaggcaaca ggaggaaaat catgatcaaa
      481 tattttacat tagataatga gaaactgcgt aataccggaa aggaaactga ccaaacgatc
      541 tggtattgtg tagaacgtcc taatcaagaa gagattcatc agctgacaca tcaatttgaa
      601 attccgtcag attacatcac ttccatattg gacgatgcag aaaattcccg tacagaagaa
      661 ttcaatcaat caaagttgac aaagccagcg ttattattgt tacaatatcc ttttgctaaa
      721 acaagtccta gcggttattt tcaagtggat acatacccgc tctctattat tttgacgccc
      781 aaaaagaagc ttatcacaat tacgaatcat gaaccactgt ttttaaagaa agtctttagc
      841 cagacctttc caaaaagtga tatttcgcct aatctcaata tcttctttca attcatttgg
      901 caggtcacac tctcttacaa cagttattta gctacgcttc aatctcaatg cgatactttg
      961 gaagccaaac ttcaagtatc cactgaaaac aaacaacttt atcaaatcat ggatatccaa
     1021 aaaagcctcg tttatttcag agaagcgact tctgctaatt tagatgcatt gaaagaacta
     1081 gcgcaaaata aacttttcca agaaaatcat gctttgaaaa atcatttacg tgatgtattg
     1141 gtggaaacag agcaggcaat gactacttct cgcattcaat taaaactagt agaacaaatg
     1201 aatcagactt t
//
LOCUS       DAZULY010000362         1195 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000362 DAZULY010000000
VERSION     DAZULY010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.373"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1076
                     /locus_tag="ACG81A_002823"
     CDS             <1..1076
                     /locus_tag="ACG81A_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS5016100.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DAZULY010000363         1194 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000363 DAZULY010000000
VERSION     DAZULY010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.374"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            28..1005
                     /gene="guaC"
                     /locus_tag="ACG81A_002824"
     CDS             28..1005
                     /gene="guaC"
                     /locus_tag="ACG81A_002824"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFS5016101.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
BASE COUNT          405 a          192 c          264 g          333 t
ORIGIN      
        1 ggatgtgaac atcaggagga taatgtaatg aaagtatttg attacgaaga tattcaatta
       61 attcctaata aatgtatcgt aaatagccgt tctgaatgtg atacaacggt cacattaggt
      121 aaacatacat tcaaaatgcc agtagtgcct gccaacatgc agacgattat tgatgaaaca
      181 atcgcagaat tcttagcgga aaatggttat ttctatatca tgcatcggtt tgatgaagca
      241 gctcgaattc cgtttatcaa aaaaatgaaa aagcgtggat tgattacctc gatcagtgtt
      301 ggcgtcaaga aagaggaata ttcctttata gagaagttgg cagaagaatc gctaaatcct
      361 gattatatca ctatcgacat cgcgcatgga catgctaact cagttattga tatgattcag
      421 catattaaaa aatatttgcc tgaaactttt gtgattgccg gtaatgttgg tacaccggaa
      481 gccgtccgtg aattggaaaa tgcgggcgca gatgcaacaa aagtcgggat tggtccagga
      541 aaagtatgta ttacaaaaat caagacaggc tttgggactg gaggctggca actggcagct
      601 cttagatggt gtgcgaaagc agctcgcaag ccgatcatcg ctgacggtgg gatcagaaca
      661 catggcgata ttgcaaaatc tgtccgtttt ggtgcgacaa tggtcatgat tggttcattg
      721 tttgccggtc acgaggaatc tcctggcgaa acaaaagtcg aaaatggaat cgtgtataaa
      781 gaatactttg gttctgcttc agaattccaa aaaggcgaaa aacgaaatgt tgaaggaaag
      841 aaaatctgga tccaacacaa aggatctttg aaagatacac tagtggaaat gcaacaagac
      901 cttcaatcat ctatttctta tgctggagga agagatctgg aagccattcg aaaagttgac
      961 tatgtcatcg tgaaaaattc gatttttaat ggagacacaa tctaaaaaat aaataaaata
     1021 ttaggatgtt gtgaaaaagt tgtcctgcat taagtaataa gaacagccaa acaaaaatag
     1081 ttctttatat gttttgtttg gctggaatta ttatcgcaga tacaagcttt tgaacatcat
     1141 tattcagaat aagggactgt gataagattt ttgtcacagt cccttatgtt ttgc
//
LOCUS       DAZULY010000364         1186 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000364 DAZULY010000000
VERSION     DAZULY010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.375"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..969
                     /locus_tag="ACG81A_002825"
     CDS             <1..969
                     /locus_tag="ACG81A_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFS5016102.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          399 a          211 c          239 g          337 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcag
//
LOCUS       DAZULY010000365         1178 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000365 DAZULY010000000
VERSION     DAZULY010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.376"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..993
                     /locus_tag="ACG81A_002826"
     CDS             <1..993
                     /locus_tag="ACG81A_002826"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFS5016103.1"
                     /translation="TILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFYMQQEL
                     SNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNVMGTQN
                     VIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIFSCVRF
                     GNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSKFGETY
                     VLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEIERSYE
                     NDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVTW"
     gene            1005..>1178
                     /locus_tag="ACG81A_002827"
     CDS             1005..>1178
                     /locus_tag="ACG81A_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFS5016104.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQ"
BASE COUNT          461 a          156 c          223 g          338 t
ORIGIN      
        1 acaatattag taactggcgg aactggttcc attggaagtg aaattgttcg tcagctttta
       61 aaatatgaac caaaagctat cagaatttat agtagaggtg aagaaaaaca attttatatg
      121 caacaagagc tctcaaatca cactaatttg agatttttaa ttggtgatat cagagactct
      181 caaaggattg attatgcatg tcgtgatgtg gatattattt ttcatgctgc cgcaatgaaa
      241 cacgttccag caagtgaata taatcctatg gaagctgtaa aaacaaatgt gatgggaact
      301 caaaatgtaa tagatgcagc gatcaaaaat aatgtaaaac attttgtagg tatcagtaca
      361 gataaagtag tgagtcctac taacaccatg ggtgcgacaa agttattgag tgaaaaatta
      421 ataatagctg ccaatcagta taaaggaaaa acagaaacaa tattttcttg tgtaaggttt
      481 ggaaatgtca tggggtcaag tggatctgtt attcctcttt ttagtaaaca aataaaaaac
      541 aataaagata ttactttgac agatgttgat atgacacgat ttatgatgtc aataccggat
      601 gctgcaagat tagttctgtc tgctgctatg aattcaaaat ttggagaaac atatgtctta
      661 aaaatgccgg caatcaatat tttaacttta gctcaatcat taataaaaat atataatgat
      721 acacatgatg aaaaatatag aggtgaaata aaagttgtag gtatcaggcc aggagaaaaa
      781 ttatatgaag agctaatgac tacagacgaa attgaacgct catatgaaaa tgataaaatg
      841 tatatcattc caccacacta tcatgctact gacgaactgt acaaagatta tgaaaaaatt
      901 aaaatcactg catactcttc agatgcaaca gaattaatga gtggacagga atttattaga
      961 atcgcaaaag agaataatgt cgtgacttgg taaggagttt tatgatgaaa tcagaagtat
     1021 atgtaattgc agaaattggt gtaaaccata atggaagtct agatttagct ttagaaagta
     1081 ttgataaagc taaagaatgt ggagctaacg cggttaaatt tcaaacattt aaaacgaata
     1141 agctagtttc caaaaaggct ccaatggcaa aatatcag
//
LOCUS       DAZULY010000366         1178 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000366 DAZULY010000000
VERSION     DAZULY010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.377"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            62..913
                     /locus_tag="ACG81A_002828"
     CDS             62..913
                     /locus_tag="ACG81A_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS5016105.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            930..1175
                     /locus_tag="ACG81A_002829"
     CDS             930..1175
                     /locus_tag="ACG81A_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016106.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT          404 a          230 c          233 g          311 t
ORIGIN      
        1 ttcttttttt cgaaaaatgc gctacactta tagttgaacg aataaaaaag gagctgaaag
       61 tatgaattca ttaacaagca cttatcgtct atcaaatggt tacgaaatcc cggtagtagg
      121 attcggcaca tggcaaacac ctgatgggga tgtcgctgtt tcctctgtta aagaagcgct
      181 ggctgcaggt tatcgccata tcgatactgc ccaaggatac aagaatgaag aaagcgtcgg
      241 ccaagcgatt aaggaaagtg gaattcctag agaagagata ttcttgacaa caaaattatg
      301 gaatgcaaac cacagctatg aacttgtgat gtcttctttt gaagaatcat tgaagaagct
      361 ccaaacagat tatttagatc tgtttttgat ccactggcca aaccctgttg cttttcgtga
      421 taattgggaa caagcaaatg cagatacatg gcgagcattt gaagaactgt atgaagcagg
      481 taaaatcaaa gcaatcggtg tgagcaattt cttaccccac catctagata cactagcaaa
      541 aacagcaaaa attatgccga tggtcaatca agttttcctt gcaccaggcg aattacagac
      601 aactgtagtt gagtatgcaa aaaaacatga tatgatcctc gaagcatata gtccattagg
      661 cactggtaaa atcttcgatg tgcctgaaat gaaacaaatc gctgaagcac atgacaagac
      721 gattgcacaa gttgctttgc gttggtcgct gcaacacgaa ttccttcctc ttcctaagtc
      781 agtgacacca agcagaatca aagaaaatac tgaattattt gattttgaat taacagaaga
      841 agaaatgaaa caaatcgatc agttagatgg tgtagtcggc aaagcaaaag acccagatac
      901 tacccaattt taagtcgaaa ggtgatttta tggatccaat cgaacgcttg aattctctct
      961 cggaagaagt tacccagact tttcattccg attttgtctt tttgatcgat gcagagaaaa
     1021 tccaacattt tcctgcaaga aactggacac atgatcaaat catcgaagaa ctgaaaaaac
     1081 ggttcgatca ttcgctaatg gtcaaaccct ggcacgaaca tgaagtcatt tattcacctg
     1141 aactacctgt ttttgccttg ataccgaaaa aatagaaa
//
LOCUS       DAZULY010000367         1160 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000367 DAZULY010000000
VERSION     DAZULY010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.378"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1141
                     /locus_tag="ACG81A_002830"
     CDS             14..1141
                     /locus_tag="ACG81A_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS5016107.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          419 a          200 c          224 g          317 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa
//
LOCUS       DAZULY010000368         1158 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000368 DAZULY010000000
VERSION     DAZULY010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.379"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..119
                     /locus_tag="ACG81A_002831"
     CDS             <1..119
                     /locus_tag="ACG81A_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFS5016108.1"
                     /translation="LILDIDSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            124..1071
                     /locus_tag="ACG81A_002832"
     CDS             124..1071
                     /locus_tag="ACG81A_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS5016109.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          373 a          171 c          245 g          369 t
ORIGIN      
        1 atctaatttt agatattgat agtgaagaag attttaaatg gttagaattt ttgtttccta
       61 aatttttaga agattcttcg gagcttcaag atatctataa ttatttatat cggaggtaaa
      121 aggatgagct gttatcttat caccggtggg gctggcttca ttggttctac cttagccaat
      181 catttaggag aagaacatgt ggtaatcgtc gtcgatgact tatcaatggg aaaagtagaa
      241 aatcttgata tgacgaaaaa tatcactttt attgagggag atgtcgctga gaaatccttg
      301 atggaagaga tcatgaaagc taataaattt gattatatct ttcatttagc agcagttgct
      361 agtgtggcag attcagttgc ccgaccagta gaaacacatc gtgtgaattt tgaaagtgta
      421 ttaatgattt tagaattgat ccgcaagtat caacctgatt taaaacggat cgtgtttagt
      481 tcttcagcgg ctgtttatgg ggatgaaccg actttaccaa aaaaagaaga gtccgtgatt
      541 cgcccattga cgccttatgc ggttgataaa ttcgctgcag aacaatatgt gctagattat
      601 tgtcatttat atgatgtacc cggtagcgca gttcgttttt tcaatgtcta tggtccaaat
      661 caaaatccga attctccata cagtggtgtg atttctattc tcgtagatcg ctataagaaa
      721 caaatcgtag gagaagcaac ttcatttaca ttatttggtg atggaagcca gtctagagac
      781 tttgtttata tagatgatgt aatccaagca ttacttttag ttgcgaatga ggaagctgca
      841 ctggggaaac aattcaatgt tggaacgggt aaagcaacca cacttttgga attgattcat
      901 gcaatcaatg aaattctcgg tgtagaattg acacttgaat acaaagaaga gcgttcgggt
      961 gatatccatg actctctagc agatattact aagatccagt caattggcta tcaaccgaaa
     1021 tatggtattt taagtggaat gactaattat ttgaaaactg aaatcaatta aaacatacta
     1081 agataagatt agagggtaaa ttcaggctct ttgtcaataa ggactgatga gttttgtaaa
     1141 atcaaatctg ggatagaa
//
LOCUS       DAZULY010000369         1155 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000369 DAZULY010000000
VERSION     DAZULY010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.380"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..963
                     /locus_tag="ACG81A_002833"
     CDS             <1..963
                     /locus_tag="ACG81A_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFS5016110.1"
                     /translation="PINGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRN
                     EAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLSDGTNHSHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          394 a          220 c          228 g          313 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttaccaa gtcggacaac ctggttatgt acagtacggt
      241 gctggttcat atgctaatgc caatagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaactt ttgaaaaaaa ctataaagca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac attagacacg ccttataacc aaccaggaat caaatcacat
      421 ttatgggtaa ctcaaaacat ttggggcgat cacactgatc cgtatggata cttatcagaa
      481 atgggtgtaa gtaaggaaaa actggcttac gatttagctc atggatttac agacgaaaac
      541 ccaacgactt ctgagaacaa gcctgtcatt gatccaaccc gagcaggtgc agcaaatcca
      601 actttatcag atggaacgaa ccattctcac attgatcagt ttggagaaat cgaaaatgcg
      661 aacttacacg tcgctggatg gcacatcgct aactataagt acgagtatat tttcattatg
      721 gactacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagaccggat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaacaacc agtac
//
LOCUS       DAZULY010000370         1148 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000370 DAZULY010000000
VERSION     DAZULY010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.381"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(187..1002)
                     /gene="thiD"
                     /locus_tag="ACG81A_002834"
     CDS             complement(187..1002)
                     /gene="thiD"
                     /locus_tag="ACG81A_002834"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFS5016111.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          297 a          254 c          188 g          409 t
ORIGIN      
        1 ctgttttttg aacttgttcc ttgtgactcg gagctggacg gcaggctcac gacctctttc
       61 ttaaacggtg ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt aaaaacataa
      121 gaagctgttt ttacttggct gttcttatta cttgatgcag ggcaactttt tcacaacttc
      181 tttcttttac tctaattcac cagctgccca atgattgatg gggccatatt tgtgccctac
      241 atttatttct tcacgaattg cacggtttac atagtctttt gccaatcgga tcgcttgttc
      301 aactgttttt cctttcgcta actcagctgc aatacatgca gatagcgaat cgccagttcc
      361 gttgatccgg tcggtaggat aaaatggtgc atttaaccaa aatgattttc ctgattcgaa
      421 caatacgtag tcattgactg tttcttgctg cgcattttta gcatgatgtc ctttgatcat
      481 aacgtttttt gcacccatat cttgcagttc ttttgctgct tgcacaaaat cttcttcagc
      541 tgctaatgtc cgtccagtca gcttttctgc ttcataaaaa ttaggtgtca gtacttcggc
      601 caaagggaca agcttttctt tcaagctttg caaagcagat tcttctagta aaagatttcc
      661 atgtttcgtt acgattacgg gatccacgac taatggtcca aaatcatatt tttggtaatt
      721 ttttacaact gtctcaatca actttgaatc tgctagcata cctgttttag ctgcacgtat
      781 ttgaaagtcc ttcgcaatca gttcaaattc tttgtcgata aaatctgttg gcaatgtcac
      841 acttgctccg attccgtaag aattacctgc aacacatgcg gtcatgacag aaacaccgta
      901 aacatttctt gtaaagaatg tatgcatatc tgcttgcatc cctgcgcttc catcgctatc
      961 tgatcctgcg atcgtcattg cttgtacata ctgatcttcc atcaaatcga ttcctccatt
     1021 tttataaaaa gatttttcat aatactgtta ctgtttctat catatgtttt tcaaagtatc
     1081 cctcagtaaa tataccacta ccttttcttg gtaatcaaat ttatttgaaa aaacatttgc
     1141 aataaatc
//
LOCUS       DAZULY010000371         1145 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.382, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000371 DAZULY010000000
VERSION     DAZULY010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.382"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(54..788)
                     /locus_tag="ACG81A_002835"
     CDS             complement(54..788)
                     /locus_tag="ACG81A_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFS5016112.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
BASE COUNT          402 a          220 c          205 g          318 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tatta
//
LOCUS       DAZULY010000372         1139 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.383, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000372 DAZULY010000000
VERSION     DAZULY010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.383"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..715
                     /locus_tag="ACG81A_002836"
     CDS             <1..715
                     /locus_tag="ACG81A_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFS5016113.1"
                     /translation="KIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSN
                     GIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPE
                     VLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETK
                     LRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYA
                     GVSPKQYQLGLVDNFNNIS"
     gene            732..>1139
                     /locus_tag="ACG81A_002837"
     CDS             732..>1139
                     /locus_tag="ACG81A_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016114.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLID"
BASE COUNT          426 a          124 c          200 g          389 t
ORIGIN      
        1 caaaatagat aatctgttat cacaaaatta cgatccagaa atatatctgt ttctttatga
       61 aaataaaata ctggaatatg ttaaaaatgg agatattcga aatttagaaa acatggtttt
      121 taatctgagt aatggaataa ttccaagtgt tagtggagat acgattcggt ctgaaaaaaa
      181 ttattctatc attgtttttg aaaagttagc acaaacgtct ataacattag gaatggatat
      241 aatagaagcg tatcaaagta gagatgcttt gatacaagaa aacgaattag ctgtatcgtt
      301 acctgaggtc ttaaaagtga gggattctgg gattgtttat tatactaagg aaatcggaaa
      361 aactaagata gagcatcttt ctcccttaat ttcttctgtc gttcaattca tcggcttaaa
      421 tatctataaa agaattactg taaaggaaat agctaattat ttttctgtaa gtgaaactaa
      481 attaagagaa agctttaaga atgaaatgca tattacgatt tataattata tctctaagcg
      541 aaaaatttct acggctaaaa taatgttgaa atcaaaccat actatcagtg aagtttcttt
      601 aggtttagga ttttcagatt cttcacactt ttcgagagta ttcaaaaagt acgcaggtgt
      661 atctcctaag caatatcaat taggtttagt ggacaatttc aacaacatta gctgagaggt
      721 tttattttat tatgagacta tatcatttac gtagtttgga ggagttgagg aaattggatt
      781 ttaacaaaaa atttttttgg gaaatttctg atttgagttg tttcgtagaa tctgagcgtc
      841 taagtaatgg taataatagt gtgttaaatg aatatgacaa agtagtaatt aataaagtaa
      901 aaagtttatg tgaagaagct agagaatcta tcagtcatat taatggtagc aattttaaac
      961 aaacattatt taatctgatt aagctagatg ctaaaatatc aagttattta ttctttttga
     1021 tacatgatga atttatggat tatcaaaaat tggatcagtt aattgaaaga gaaagctggg
     1081 aagattatta cgtgggtcta actttttcag aaaagttaaa caactacaag ttaatagat
//
LOCUS       DAZULY010000373         1138 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000373 DAZULY010000000
VERSION     DAZULY010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.384"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..1031
                     /locus_tag="ACG81A_002838"
                     /pseudo
     CDS             1..1031
                     /locus_tag="ACG81A_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          404 a          204 c          228 g          302 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag aagcgatcgt aacagacttg acttatgtac
      541 gagtcgggaa aaaatggaat tatgtctgtt tcattttgga tctgttcaat cgagaaattc
      601 tcggctattc ttgtggagaa cataaagatg ccgttctagt aaaaaaagca tttagccgta
      661 tcaaacaacc tctgacagag gttgagattt ttcatactga tcgtggaaaa gagtttgata
      721 accaagctat tgatgaatta ttaacaactt ttgacatcaa tcgatcattg agtcataaag
      781 gctgtccttt tgataatgcc gtagctgaat caacttataa gtcgttgaaa gtagaatttg
      841 tctatcaata cacatttgaa accttacaac aattggattt ggagttattt gactatgtca
      901 attggtggaa ccaccttcgg ttgcacggta cacttggcta cgagacaccg gttggttacc
      961 gtaaccagag attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg
     1021 aggcagtcta actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat
     1081 tgtcaaggac aatcggaata gcatacggaa agaagttctg caccttataa aatttgtc
//
LOCUS       DAZULY010000374         1136 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000374 DAZULY010000000
VERSION     DAZULY010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.385"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1103)
                     /gene="dnaN"
                     /locus_tag="ACG81A_002839"
     CDS             complement(<1..1103)
                     /gene="dnaN"
                     /locus_tag="ACG81A_002839"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFS5016115.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFI"
BASE COUNT          349 a          211 c          209 g          367 t
ORIGIN      
        1 tgaataaatt gaacgccatc ttccgttggt tccaatgtaa atggacgaat agcagagata
       61 aatttcactt tgatgctcat atctccaaat gcacgcaacg ctgctttcat ataatccgga
      121 ttgaaagaaa tatctaatgg atcgccactg cttgctgtat aactcaagct ttcttctact
      181 ttcccgattt ctggcgaatt tccgtataaa acaaccgcat ctggacgaat agataaacga
      241 acgatgttgt tacggccttc atgagaaagt aaagaagccc gttcgatcgc cgctaaaaag
      301 ctaggaacag aaaattcaac ctctgtatta aaacttgaag gaatcaaacg attggtatct
      361 ggatagtttc cttctagcaa acgggaatag aaatacatcg tttctgtttt gaacaacact
      421 tggttttcca taatgctgat ttcgacgatt tcttcttcat ttgttaatga gcgagataat
      481 tcgatcaaac tttttccagg aataacaata tcaaaatgat cagccgcttg ttctactgga
      541 atcacgcgtt gacttagacg gtgagaatca gtagctacag ctaataaaga attatcagat
      601 aaaatgaaat gcacacctgt caagattgga cgactctcat gttgtgagac agcaaaaact
      661 gtttcgttga ttagtttagt caatacgtgt acaggtaatt tcatctggtt atggctttcg
      721 acaacaggaa gatgaggata gttatctgca tctaatccat ttacgataaa attcgctttt
      781 ccagaagtga tcgctacttg tttattttct aaaacttcta aagtaaatgt ttcttcagga
      841 agtctccgaa tgatttcgct aaagaaacgt gcttgtaaaa caatggaacc agtagattcg
      901 atttgcatat ttgctttttc gttttcaaca gacaaaaaag tttcaattga tatatcagcg
      961 ttgctccccg tcaaagtcaa accttcttgt gtcagtgtga tttttacacc tgtcaaaata
     1021 gggatcgtgg ttttgcttga aatagctcgt tgaacagttt gcaattcctg cataaagcta
     1081 gctcggttta aagtaacttt catgatgtat ctcctttgaa tgtttgttta tttatt
//
LOCUS       DAZULY010000375         1124 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.386, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000375 DAZULY010000000
VERSION     DAZULY010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.386"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            16..276
                     /locus_tag="ACG81A_002840"
     CDS             16..276
                     /locus_tag="ACG81A_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016116.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(638..877)
                     /locus_tag="ACG81A_002841"
     CDS             complement(638..877)
                     /locus_tag="ACG81A_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016117.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          399 a          162 c          207 g          356 t
ORIGIN      
        1 aaggatggat gaaatatgaa taacgaaact atcattttag gaaaaaaata tatttgccaa
       61 cctattggct tgaaacaaaa agtggttgga gaagtaatca gtaaattaga aaattgtgtt
      121 gtgatctgcg tagacgaata tcatgacgtc gatcgcaatg aaatattaga aaaatgcgga
      181 aaagtagtgg caaaatatac tcacgtttat gagttggaaa caacagaata tctatataac
      241 tatagcaaca aatatgaaca tactttagtt tcataaagat ttaaataaag tgccttcaaa
      301 agcgagaaga ccaagctttt gaaggcactt tatttagttg gcagtgattg ttcatgattt
      361 ttgatttatt caaaaggttc gcctagagaa tctgtctctg taacgaggcc tggtggagaa
      421 actcgataaa aacctatact gccagaactg tcctattttc ggatagacaa actagtagtt
      481 ttaaaaagat catccgatcc aatcatttca aaaaatgcaa aactgatatc aggatcattt
      541 agctcaggct tgttatcgta aagcatttgt aaaggttgca tttagttagg agtaaacgcg
      601 aaactatcat cattatttgc tgaagaagat gaagaagcta attcagagtt tgtttgaaat
      661 aaaggagacg tgctgacagt ttctttaccc tgttttttgc agaggtagaa acttttatat
      721 cggaagtttc taccggagaa tttttctcat ttgtagaaat tgttgaatcg atactattcg
      781 aaatttgcgt atctgaaggt atgttctttg atgcagaagt cccacaagca gtaagtaaca
      841 ataaatttga taaactgata aacagaattc ttttcattat gtacacactc cttatagtat
      901 cgtttagcat acacctatgc tcagtattag gcaaaatcaa tgcaaaacta tcaagtcttt
      961 tatagtatct attagtctta gtaatacata tatggagagg gtgatatccc agttcaaacc
     1021 agatacattg gttaccatct gtattaacga ttatgcctct aaacgctttt tttatgaaaa
     1081 aatttataag tgaaaacgta ggtatgatat tatctagagg taat
//
LOCUS       DAZULY010000376         1105 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.387, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000376 DAZULY010000000
VERSION     DAZULY010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.387"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..389
                     /locus_tag="ACG81A_002842"
     CDS             273..389
                     /locus_tag="ACG81A_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFS5016118.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT          397 a          171 c          175 g          362 t
ORIGIN      
        1 gtgttctatc gaacacacag tacgttcaat aggtaaacta gctataacaa tagatattaa
       61 agttaacgga caagagggcc ttttttaggt agctaaaacg taataatgaa aatttatttc
      121 cagagatagg agtcaatcag ctagtctgat cgactgtttt aacaagttga ctttcatttt
      181 aacttttgat accatttaaa acacaaatcg taattgtttc gatttgtgtt ttttagcatg
      241 actacaatca aagaagaaag tgggtcgtcc atatggaaaa aacagatatc tcaagtgcat
      301 accgccgctt gaaaagccca aatatcaaaa cacgaaaacg tgcgctgaag atcatcaaag
      361 atgtgaaacg gaatagtggg aaaaggtaag aagaaggaaa cttaattaaa aaaatagccg
      421 aaaaacgttt ttcggctatt ttcaataatc ggtttttgtg taaggtttcc gaaagaactg
      481 ctgttgattt tccattttta acatatgata aattggtaga atctttcttt tcaaattgat
      541 accattccct aattccagtt atatagttaa ctacagaacc acaaagcacg tacgtttata
      601 aaagtattat ctgtagttat ttttcaattc cctttcaacc tcttattttg accagtagta
      661 ttgatcactt taccttttaa cacctttgtt cgtttttgaa acatttttag tcaagaatgg
      721 aaacgtaact acataatcaa aggttttgcg aaatatttca tgaaatattt catgtagaag
      781 cgagcaatac ggttgcaaat aaccattatc gtttaataag cagttacgaa agctcccaat
      841 atctctaacg ttcagctcac tccatttctt ttcttcaaaa aaatcagtga ttcgtatctc
      901 tttctatttg tcgaaaaagt taactttaaa aagtaacgcc gaaaaaatat gcaaaaaaat
      961 aaaaaagcag ttactattgc tagaataaca tcaaggaata ataagttttg gttttcagat
     1021 aatagtcgat aagttgtgta ttggaatttt agatacagta ataaagtatt tttcatgaaa
     1081 ttttgtgaga atcactatta caagt
//
LOCUS       DAZULY010000377         1104 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000377 DAZULY010000000
VERSION     DAZULY010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.388"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..1010)
                     /locus_tag="ACG81A_002843"
     CDS             complement(132..1010)
                     /locus_tag="ACG81A_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFS5016119.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT          328 a          213 c          181 g          382 t
ORIGIN      
        1 tttttgtgaa tttcagctta tttccgaagg agttgcttct gcttccgccg tttatacgtg
       61 tttagagcgt gagacaaaag tgttctttac ttttgtctca cgctctttta aactatttcg
      121 aagtactaaa cttatgaagt aagaatacct aagccagagc caatgtgctc gcctgcaagc
      181 aattgctctt ttgcttgtcg ctgtaaactc gtactttctg gataatcatg tatttgggca
      241 tctactcgtc caaagatcca agggactcca ctatcgtcct gttcatcaac tgataataat
      301 cgataatcgg ctgcaaaaag acagattttt gactcttttt tcaaccattt agtaagattg
      361 ggtgatagta accaagtatc tgtgtacatc acttggtaat ggtaatcagg aaaataaagt
      421 tcaaaaaaac gaatggcact ttcgtaacta tctcttatct tattctgaga taaatctgca
      481 tcggaaggaa tatgaagaga aaggcactgc tttccttcta atcttagatc cgtgtaccct
      541 ttcggaaaat atgtcatttc gtactccaat tcaccaatcc gaaagatcaa gccactagtg
      601 taatgccaaa tccaaaaacc acggtcaaag cgatagcttc ctgttacttg gaaatgttca
      661 tgaataaatc tagtaaaagc attcattgtt gccaaataga tagttcttgg aatcttcaat
      721 ggttgataaa tttttttcca actttgagaa gctgcatata gataaatagc taatgctgca
      781 aaaggttcct cttcatagac tttgtctatt ttctttgcag tcgcctttgc tttattagga
      841 tccattagtt catgactata ttgctcaaac acctcatcaa ggatgttttt tgcttgtaca
      901 atcatttgct ctataacgac tttgggaaga gttaaagttt ctgccagaat ttggatttca
      961 ttgcttattt tcttcatcaa atttcccctt ttcatatctt tgaacctcat tgtttaaatg
     1021 actgattatc cggacaccat atggtataaa catactttta ttgtacacta actataaaac
     1081 ttaaactttc tatgaagaaa gaat
//
LOCUS       DAZULY010000378         1099 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.389, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000378 DAZULY010000000
VERSION     DAZULY010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.389"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..999
                     /locus_tag="ACG81A_002844"
     CDS             <1..999
                     /locus_tag="ACG81A_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFS5016120.1"
                     /translation="IYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQKIS
                     SAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVLYE
                     LDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLTDW
                     TYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFDIM
                     ADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEVQE
                     ISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL"
BASE COUNT          417 a          160 c          233 g          289 t
ORIGIN      
        1 atctatttaa aaaaagcaat tctgcatatt attgacagag aaactggttc tccagtcttt
       61 tcacaaaaag agctggatct gacaaaagaa tatattcgtg attttcttca aaagaagatc
      121 caaaaaatct cttctgctca aacaaaaact ggacaactag cagaagaaag catgatcgtt
      181 gaatcactta aaatcgctca agaaaatttt gtggaagcaa gtgaagcaat cgttcagcgg
      241 tggttcgata tctaccaaga aagtgaagaa gcacctagtg cagatgtatt tgttgtcttg
      301 tatgaattag atacagtgat gtacttatct ttattgaaag tcaattatcg tgaggcttac
      361 actcactttg ttgaagcaga cgaagcaggg atcgacaacc gcttgatttt gaatcgagca
      421 attctagggg cgaaaacaca aaaagcagat gaagcattcg ctgtgaattt gactgattgg
      481 acctacgaat taattgaaaa aaaatatgag ttttctggca aaaaagagct ttatttttct
      541 ggaagagtga tcgaaagtgt tcctgcacct tctttagaag aaaatgtaaa agtgatccaa
      601 aaagtagcca agcatcttgg aaaaaaattt gagacagaag aatttgatat catggctgat
      661 ttgaaagaag ctgtctatga tacgatagaa gaaaaaggtc ggttggatca tgagatgatt
      721 gcggaaaaag tcttcaagga aaacatcacg gcaaaaatgg cttttaaaga agaagtacaa
      781 gagcaaggat ttgtcccaga agcacctcct gtaaaagaag tacaagaaat ttctgagaaa
      841 aaatttggaa aacaaaaatt caagctatca aacgggatcg aactgatcgt accagtagat
      901 gtatatcgca acccagattt gattgaattt gtgaataatc cggatggtac gatatccgtg
      961 atgatcaaaa atgtggatga agtattgaat cgtttataaa aatgactaga atattaggtg
     1021 ttcaagtacc agtgatcagt taaatgaatt gtgtcaagag aaggatcgga aaagacgaat
     1081 ataacaaaaa aagagcgtg
//
LOCUS       DAZULY010000379         1093 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.390, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000379 DAZULY010000000
VERSION     DAZULY010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.390"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..382
                     /gene="spx"
                     /locus_tag="ACG81A_002845"
     CDS             <1..382
                     /gene="spx"
                     /locus_tag="ACG81A_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HFS5016121.1"
                     /translation="TSCRKARAWLQANQLEFEEKNIFAEPLTESEIKKILAATEGGVQ
                     DIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRILIGFNEDEIRS
                     FVPREIRCVARNLIYLQNNNILNS"
     gene            396..752
                     /locus_tag="ACG81A_002846"
     CDS             396..752
                     /locus_tag="ACG81A_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5016122.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          380 a          165 c          208 g          340 t
ORIGIN      
        1 tacttcctgt agaaaagcaa gagcatggtt gcaagcgaat caacttgaat ttgaagagaa
       61 gaatattttt gccgaaccgt taacagaaag tgagatcaaa aagatcttgg cagcgactga
      121 aggaggagtt caagatatta tttccactcg gtcgaaaatt tacgaaaagc tgggcataga
      181 ttttaacgaa ttaactttaa agcaaatgat cgtcttattc aaagaatatc cttcattatt
      241 gagacgacct attttgattg atgaaaaaag aatactaatc ggatttaatg aagatgagat
      301 ccggtcattt gtccctcgtg aaattcgatg tgttgccaga aatctgatct atttacaaaa
      361 caataacatc ttaaattcat agggagggag ccttcgtgga agtgaaagtc tatggatcag
      421 cgcactgtgt aacaagtctg aaggcgaaac aatggttaga acaccatcaa ttgccttatc
      481 aatttgttga cttagaagaa cgcgatctaa gtacagaaga agcagaagaa ttatgcagtt
      541 ttgaagaagt gcaagcagaa cagctatttg cgacttggtc tgaggctttt cacaacattg
      601 cgattgacct ttcctgtgta ggaaaaaaac aactgatttt tttatgcaca caacatacca
      661 atctcctcag acgtcctttg attattatcg atgaagttct atttattgga tacaatgagc
      721 agttgatgga acagcttatc ttaaataaat agactttaac tgttttaatc aagtcactga
      781 gcatttcata cagttgtaac agtcgaacac ttagcatcaa gcgtgttatg tgttcttttt
      841 ttgactttat ttttctacta tagagacatg ctcaatttat gtggtcaaaa ttcaaatcta
      901 ggtggggata tagagttaaa aatatattga catgtgtaaa tttacatgat atattcattt
      961 ctgttgtttt aagtagcaaa gcaataaaat agtattttaa aaaacatata aaatagttgt
     1021 tgacatgtta gaaatgacat gataagataa ctgagttgtt aaaaaagaac cacttgcgaa
     1081 gcttgattct atc
//
LOCUS       DAZULY010000380         1083 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.391, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000380 DAZULY010000000
VERSION     DAZULY010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.391"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..>1083)
                     /locus_tag="ACG81A_002847"
     CDS             complement(243..>1083)
                     /locus_tag="ACG81A_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS5016123.1"
                     /translation="SIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLASELG
                     IADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVFESS
                     ISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIATSHS
                     TDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSSLAK
                     LGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHTISE
                     IKE"
BASE COUNT          342 a          183 c          191 g          367 t
ORIGIN      
        1 acaaaaataa taaaaaaaag aaaagataaa ttttttacta ctgtatacta actttgttat
       61 cgcatctaac aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat
      121 acgttctaac ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac
      181 taccaaatat ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg
      241 tgctattctt tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct
      301 ttttcgttgt aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata
      361 cgggaagcta aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca
      421 tcgccaagtt tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca
      481 gtttttctta cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc
      541 aaaacagcac agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc
      601 gtgctgtgag aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca
      661 atctctgaac cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc
      721 gcttttttta gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta
      781 gactcgaaca ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg
      841 atagaaatag cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat
      901 ccattataac caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca
      961 cttgctaaat tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg
     1021 gcgatagcct gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg
     1081 gag
//
LOCUS       DAZULY010000381         1080 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000381 DAZULY010000000
VERSION     DAZULY010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.392"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="ACG81A_002848"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="ACG81A_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFS5016124.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          321 a          221 c          183 g          355 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
//
LOCUS       DAZULY010000382         1075 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.393, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000382 DAZULY010000000
VERSION     DAZULY010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.393"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..1054
                     /locus_tag="ACG81A_002849"
     CDS             110..1054
                     /locus_tag="ACG81A_002849"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFS5016125.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT          379 a          164 c          226 g          306 t
ORIGIN      
        1 tcaaaaatta taataacaac tattataatt aacatgttga taaaatgaat aaacaagact
       61 atacttttca tgaaagtaac aaatagttat gaaaaggagc tgtttattca tgaaaaaaac
      121 aagtcgtaaa gtagtaatcg tcggtacagg ttttgttgga acaagtatcg cttatgcaat
      181 gatcaaccaa ggaatctcta atgaattagt attgatcgat gtgaatcaag aaaaagcaga
      241 aggggaagca ttggaccttt tagatggtat ggcttggggc gatgaaaatg tggctgtatg
      301 gtcaggtggc tatgaagaat gtaaagatgc tgatatcgtt gttatcacag ctggtatcaa
      361 ccaaaaacct ggtcaatctc gtttagattt agttaaaaca aatgcgtcaa tcatgcgcca
      421 aatcgtaaaa gaaatcatgg gatcaggatt cgacggtatt atcgttgttg cttcaaaccc
      481 agtagatatt ttgacatata tcgcttggaa cgaatctggt ctgccaacat cacgtgttat
      541 cggtactgga acaacattgg atacaactcg tttccgtaaa gaaatcgcat tgaaattaaa
      601 agttgaccca cgcagcgtcc acggctatat cttaggtgaa catggtgatt cagaagttgc
      661 tgcatggtca catacaactg tcggtggtaa accagtgttt gaaattgtag aaaaagacca
      721 tcgtattgcc caagatgaat tagatgtgat tgctgataaa gtccgtaatg ctgcttatga
      781 gatcattgat cgtaaaaaag caacttacta tggtattggt atgagtactg cacgtatcgt
      841 aaaagctatc ttgaataacg aacaagctgt attgcctgta tctgcttatt taactggtga
      901 atacgacgaa aaagatatct ttacaggtgt gccatcaatc gttgacgaaa atggtgttcg
      961 tgaagttgtt gaattatcaa tcaacgaaga agaaaaagca atgttcagca aatcaacaag
     1021 tgctttaaga gaagtattga acactgtatt ataagaaaca atgaatatga actaa
//
LOCUS       DAZULY010000383         1059 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.394, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000383 DAZULY010000000
VERSION     DAZULY010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.394"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..993)
                     /locus_tag="ACG81A_002850"
     CDS             complement(37..993)
                     /locus_tag="ACG81A_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016126.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgt
//
LOCUS       DAZULY010000384         1040 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.395, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000384 DAZULY010000000
VERSION     DAZULY010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.395"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..436
                     /locus_tag="ACG81A_002851"
     CDS             86..436
                     /locus_tag="ACG81A_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFS5016127.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            535..>1040
                     /locus_tag="ACG81A_002852"
     CDS             535..>1040
                     /locus_tag="ACG81A_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HFS5016128.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFI"
BASE COUNT          296 a          167 c          198 g          379 t
ORIGIN      
        1 gtttttttga ataatcgctt tttcttctct ttttcttata tactataagt agtaaataat
       61 ttgaaaaaaa gaaaggtgac tgattatggc gcaaaatcgt atttttacga caagttttgc
      121 aagcgtttat cctatgtatg tagcaaaagc ggagagaaaa gatcgtataa aagaggaagt
      181 agatgaaatc atttgttggc tgacgggtta ttcccaaaca gaactagaat atcaactaca
      241 aaaacaagtg actttcgaac aattttttgc ggaagcccct gtccccaatc cttcacgttc
      301 cttgattaca ggtgttgttt gtggtgtacg agtagaagac atcgaggata acttgatgaa
      361 agaaattcgt tatttagata aaatgatcga tgagctagca aaaggcaaaa aaatggaaaa
      421 gatcctcaga agttgatttt tccagagata ttcttctcct tcgctcattt tgtaacattt
      481 aatttggtaa aatgactagt tcacgttaaa ataagtcaaa ggaggttttc ttatatgttt
      541 gagacgatta aacaaaatat tcaaaaatac gccttattaa gagctgctgt ttatattatt
      601 tctgggattg cgattgtgct aaacccttct gctgtttttc attttatcgg ttatctgatc
      661 accgcctatc ttgttttgct tgggttgttg aatctttttg aggcttaccg ttttaaaagg
      721 aaaacaggtt cttggagtat cggtctgttc agtggtcttt ttttcctgat cttagctctg
      781 atcgtctatg cttttgcacc tgcaatcgtt tctatcctgc cgattcttct tggattagct
      841 gttatcatca acagcttgtt ccagttgttc ttcagcatga atacaaaagc taaaagttgg
      901 agtgtttata gtagtattct gttgttgatt ggcggattcg tcttattgtt caatccattc
      961 aaaagtttga tgcttctatt ccaaatattc ggctttattt tgattttcat gggcatccag
     1021 gagattatca atttcatcag
//
LOCUS       DAZULY010000385         1035 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.396, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000385 DAZULY010000000
VERSION     DAZULY010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.396"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            163..>1035
                     /locus_tag="ACG81A_002853"
     CDS             163..>1035
                     /locus_tag="ACG81A_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5016129.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNN"
BASE COUNT          360 a          180 c          189 g          306 t
ORIGIN      
        1 gttgatttac caacatgttt agacattttc ctctaggaaa aaattaacta gcaccacggg
       61 tttttattac gccgattgta aaattaagct agacaactaa aaaagtcatt tgtgctacac
      121 tcaaaatagt tccgaccaaa gaattataag gagtgagtac aaatgaccta tacccatctt
      181 acaacggatg aacttgtaat aatagagtct tatttcaaaa tgaatcaatc tgttgctaaa
      241 actgcccatt gcttgaatcg ttcaagacaa acgatccata aagtatatct gttcttcaag
      301 caaggaaaat cagccttaga atattatcaa cagtataaga aaaacaaatc aaactgtggt
      361 agacgtccgc ttgttttacc cgaggaacaa tcagaatata ttcaaagaaa ggttgttcaa
      421 ggatggacac ccgatgtgat tgttggtcgt gcagcgtttc ctattagttg ttctgctcgt
      481 accatttatc gtatgttcaa aaaggggcta tttgattctt ctgacttacc gatgaaaggc
      541 aagcgtaaac cgaatggaca tcaagaaaga cgtggaaaac aaactttccg ccgctctatt
      601 catgaacgtg aaaaggatta tagccaattc tcaaatgagt ttggtcacct tgaaggtgac
      661 actatcgtag gtctgaaaca taaaagtgct gtaattacct tagttgaacg attatcaaaa
      721 gttatcatca cattgaaacc gtgtggtaga caagcgattg atattgaaaa aaaattaaat
      781 caatggtttg aatctgtacc gaaaaaccta ttcaaatcca tcacttttga ttgtggaaag
      841 gaattttcaa attggaaaca gatcagtaat gtcaatgata ttgccattta tttcgctgat
      901 ccaggaacgc cgtctcaaag aggcctaaac gagaattcta acggattgtt acgtagagat
      961 ggtttattga aatctatgga tttcaattca gtagatgaat tttttattca atctgtcgca
     1021 tctaaacgaa ataat
//
LOCUS       DAZULY010000386         1033 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.397, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000386 DAZULY010000000
VERSION     DAZULY010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.397"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(190..894)
                     /locus_tag="ACG81A_002854"
     CDS             complement(190..894)
                     /locus_tag="ACG81A_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016130.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT          294 a          186 c          188 g          365 t
ORIGIN      
        1 actccaagat atcttcaggt agaagagaag tcgcctaaca ttgtaggatc ataggtgatt
       61 tgttggacag gtcaagctga tatttgagcc tgatgttaga aggaatttct ttttacttgc
      121 tgtagaaatt attatatgac taaaaaggct gggattttaa atcccagcct tttatgacaa
      181 atagaatttt tatcgatcag taatcgctgt cgctgcaaat tgttcgatca aaggaatcaa
      241 agtcaatttg actttgtatt tccgttcgag tgctttttcg ataacgaact cattttcagt
      301 agtcatagaa atagttgcta atttacgagg ttcatcagca tttttttcat taataactac
      361 ttcatgttca ttttgttctg tgtaaaattt aggatactca gcaacggctt ttgaaaaatc
      421 agcaaaactt aaagcggcat tcgtatcagt tgagcgtgta ttttcagttg tgtccgcttt
      481 tggtttttcc gttttttctt ctggcacact gactgtttct tctgtaactg gtgtcgcaac
      541 agcttgcgca gttggttcat cgtttttcaa tcgttgatca ttccagtaag acacgatttc
      601 aataagaaga ttgtcacgga atcgttcgaa aatcccgttt tctcctgaac gaattctctt
      661 taatacagta ttgaagttgt catcgattgg cacgttgatc cgtgtatctt catttgtttt
      721 cccagctaat ccgatcgtta agatttgacg gtttttggga tcaagcatga cttgtaacgg
      781 caaaccataa cgtcgcaagc tctcttcttc tagagaacgg aataattgtc ctcttacacc
      841 atccaatgaa ggacggaatt gatttaataa tttttgtttc tgttttttct tcatatgttc
      901 tctccttcgc tcataccctt tcgattataa gaaaaaaaca gccaaatgaa aaggagtaat
      961 gcaaaaaaac ttgcgtatac gcaaattaca acagaaaaat ttgcgtattt tcccgttttt
     1021 tgaaagaaaa ata
//
LOCUS       DAZULY010000387         1010 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.399, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000387 DAZULY010000000
VERSION     DAZULY010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.399"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..848
                     /locus_tag="ACG81A_002855"
     CDS             <1..848
                     /locus_tag="ACG81A_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS5016131.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          329 a          195 c          202 g          284 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt
//
LOCUS       DAZULY010000388          994 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.400, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000388 DAZULY010000000
VERSION     DAZULY010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.400"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>994)
                     /locus_tag="ACG81A_002856"
     rRNA            complement(<1..>994)
                     /locus_tag="ACG81A_002856"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DAZULY010000389          986 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.402, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000389 DAZULY010000000
VERSION     DAZULY010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.402"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..704
                     /locus_tag="ACG81A_002857"
     CDS             <1..704
                     /locus_tag="ACG81A_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS5016132.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          333 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataat
//
LOCUS       DAZULY010000390          985 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.403, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000390 DAZULY010000000
VERSION     DAZULY010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.403"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..913)
                     /locus_tag="ACG81A_002858"
     CDS             complement(26..913)
                     /locus_tag="ACG81A_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFS5016133.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          284 a          206 c          179 g          316 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaatt
//
LOCUS       DAZULY010000391          979 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.404, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000391 DAZULY010000000
VERSION     DAZULY010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.404"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>979
                     /locus_tag="ACG81A_002859"
     CDS             <1..>979
                     /locus_tag="ACG81A_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFS5016134.1"
                     /translation="SIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRACRS
                     CGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYF
                     VQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSKKIL
                     PRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEF
                     LVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKSQAE
                     KLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPP"
BASE COUNT          323 a          191 c          194 g          271 t
ORIGIN      
        1 tctatcaaaa aaatgctgag aataacagag aaagatttga tgattacaga ggtctcttac
       61 gagacccttc agaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc tactcctcgt
      121 gcttgtagaa gttgtggttc tactgtggta gatggaaacg ggaaagcaat tatagtgaaa
      181 aatggaaaaa aggaaacgat tgtccgtttt gaacaataca atcatatgcc tttggttatg
      241 cgcctaaaaa agcaacgcta tacctgtaaa aactgtcgca cccattggac ggctcaaagt
      301 tattttgtcc aacccagaca ttcaatcgca aatcatgtta gatataaaat tgcttcttta
      361 ctgactgaaa aagtatcttt atcttttatt gcgaaaagct gtcaggtatc tttgaccacc
      421 gttattcgta cattgaaaga gtttaaaagc tatttaccaa agcaatctaa gaagatcctc
      481 cccagagtat tgatggttga cgaatttcgt tcgcatgctt ccatagaaga taagatgagc
      541 tttatttgcg cggatggcga aacaggaaaa ttaatagatg ttttgcctac gcgtaaatta
      601 cctcgattaa caagctattt cttaggttgt accaatccag aagaagtaga attcttggtg
      661 acagacatga acgccgccta cttccagctc accaaacgtg ttctgccaaa tgcgaaagtc
      721 gtgattgatc ggtttcatat tgtcaaacac atgaatcaag cgttcaatga gttgcgtatc
      781 cgtgaaatga atgaacttcg taaagccgga cagaaaagcc aggcagaaaa actgaaaaag
      841 aactggcgct ttttgctaaa aaatcgtgca aacatcaacc attatgaata caaaacatgg
      901 aaaagtttcc gagcaccaaa atacccattt cttactgaag caatgatgat tgatcgattg
      961 cttgagtttt ctccgcccc
//
LOCUS       DAZULY010000392          967 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.405, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000392 DAZULY010000000
VERSION     DAZULY010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.405"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            87..356
                     /locus_tag="ACG81A_002860"
     CDS             87..356
                     /locus_tag="ACG81A_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS5016135.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            349..606
                     /locus_tag="ACG81A_002861"
     CDS             349..606
                     /locus_tag="ACG81A_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS5016136.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          372 a          104 c          178 g          313 t
ORIGIN      
        1 tataagaagt tagaatatta atcatttgct taattgtaca atataatgta caattgtttt
       61 atagaaataa ataaggggtg aaaggaatgg aagcagtagc ttattcaaat ttccgccaaa
      121 atttacgtag ttatatgaaa caagttaatg aggatgctga aacacttatt gtaacaagta
      181 aagatgtaga agatacagtt gttgtattat caaaaagaga ttatgattct atgcaagaaa
      241 cgttgagaac actttctaat aattacgtca tggaaaaaat tcgtcgagga gatgaacaat
      301 tctccaaagg tgcatttaaa acacatgact taatcgaggt tgaatctgat gattaaggct
      361 tggtctgatg atgcttggga tgattatctt tattggcatg agcaaggaaa caaaagcaat
      421 ataaaaaaga ttaacaagtt aataaaagat atcgatcgtt ccccctttgc tggattagga
      481 aaacctgagc cattaaagca tgatttatct ggaaaatggt ccagaagaat tacagatgaa
      541 catagactga tatatagagt tgaaaatgaa acgatattta tttattctgc aaaagatcac
      601 tattaaccaa tcggaagtaa ggaaagggtc agaaacttaa aagtttttga tccttatttt
      661 atttacccta gtcatttaaa aagctaatat agcttagtgt tgattgttat taatgaatgt
      721 gtttgttacg cgtattacgg atataaggtt agtaaaatca tttctaaagt tgaggaaaag
      781 taaatataaa tggcttaaat ttcaacaatt tgaagttgaa tagatatgtt ataatactat
      841 tgtagtgtgg gatgttagtt actaaaggat gacgcttata tatatgactg aatagaataa
      901 gcaataggtt taataatcta ttttaaattt tttgtactag ttttagttaa ttagcaaaaa
      961 caacaaa
//
LOCUS       DAZULY010000393          959 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.406, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000393 DAZULY010000000
VERSION     DAZULY010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.406"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..511)
                     /locus_tag="ACG81A_002862"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="ACG81A_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="ACG81A_002863"
     CDS             complement(570..>959)
                     /locus_tag="ACG81A_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5016137.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DAZULY010000394          957 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.407, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000394 DAZULY010000000
VERSION     DAZULY010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.407"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            324..957
                     /locus_tag="ACG81A_002864"
                     /pseudo
     CDS             324..957
                     /locus_tag="ACG81A_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          324 a          181 c          177 g          275 t
ORIGIN      
        1 ccagaaagaa atgaacacct tcaattatct aaatctcgaa aatggttaga tgtaatcatc
       61 aggaaaaaca atcttaaacg aattactatt catggtctta gacacacaca tgctagcttg
      121 cttttagcaa gctagtgcaa atattaaaga tgtgcaagag cgtttaggcc actcttctat
      181 tcaaatcatt atggatctat atattcatat tacagacaaa agaaaagaag aaaccgtagc
      241 gcaattcgca aaatataggc tctataattt atgggactga tgaatcaaaa ttgattcttc
      301 agtcccattg ttattttagg tatatgattc gtacattgaa agagttgaaa aactatcttc
      361 cgaagccgtc aaaaaggacc ctgccaaaag tattgatggt ggatgaattt cgttcgcacg
      421 cttcaattgt ggataaaatg agctttattt gtgcggatgg cgagactgga aaactcattg
      481 atattttgcc tacacgaaaa ttgtctcgat taacacacta ttttagtcgc tgtgcaaatc
      541 ctgaagcggt ggaatattta gtgacagata tgaacgccgc ctacttccaa ctgacaaaac
      601 gtgtcttacc aaatgcgaaa gtcgtcattg attgttttca tatcatcaaa catttgaatc
      661 aggcgttcaa tgattttcgt gtacaagaaa tgaaaagatt acaccagtta gggcaacgac
      721 gggaagccga gaaagtgaaa aagaactggc gctttcttct aaaaaaccgt caaaaaatca
      781 atcactatga ttacaaaaca tggcgcagtt ttcgtgcacc taaatacccg ctactcactg
      841 aagcaatgat gattgatcga ttgctggcat tttcaacgcc tttaaaggac gcctatactt
      901 ttttccatga gctgaccgaa gcctttagag acaaggaatc agacttattt tttacct
//
LOCUS       DAZULY010000395          948 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.408, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000395 DAZULY010000000
VERSION     DAZULY010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.408"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..>948
                     /gene="bsh"
                     /locus_tag="ACG81A_002865"
     CDS             6..>948
                     /gene="bsh"
                     /locus_tag="ACG81A_002865"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFS5016138.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPI"
BASE COUNT          328 a          126 c          171 g          323 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaatta
//
LOCUS       DAZULY010000396          936 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.409, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000396 DAZULY010000000
VERSION     DAZULY010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.409"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..929
                     /locus_tag="ACG81A_002866"
     CDS             147..929
                     /locus_tag="ACG81A_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFS5016139.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
BASE COUNT          324 a          136 c          198 g          278 t
ORIGIN      
        1 tctgtagaca aattgtgaaa ggatgtactt aaacgctaac ggtcagcttt attgaacagt
       61 catttaggta tatgtccaat ctagggtaag taaattgagt atcaatataa actttatatg
      121 aacataatca gttacgaggt gaaatcatga gcaatttgat taacggaaaa ataccaaatc
      181 aagcgattca aacattaaaa atcgtaaaag atttatttgg aagttcaata gttggagtat
      241 atctatttgg ttcagcagta aatggtggtt tacgcattaa cagcgatgta gatgttctag
      301 tcgtcgtgaa tcatagttta cctcaattaa ctcgaaaaaa actaacagaa agactaatga
      361 ctatatcagg aaagattgga aatacggatt ctgttagacc acttgaagtt acggttataa
      421 ataggagtga agttgtccct tggcaatatc ctccaaaaag agaatttata tacggtgagt
      481 ggctcagggg tgaatttgag aatggacaaa ttcaggaacc aagctatgat cctgatttgg
      541 ctattgtttt agcacaagca agaaagaata gtatttctct atttggtcct gattcttcaa
      601 gtatacttgt ctccgtacct ttgacagata ttcgaagagc aattaaggat tctttgccag
      661 aactaattga ggggataaaa ggtgatgagc gtaatgtaat tttaacccta gctcgaatgt
      721 ggcaaacagt gactactggt gaaattacct cgaaagatgt cgctgcagaa tgggctatac
      781 ctcttttacc taaagagcat gtaactttac tggatatagc tagaaaaggc tatcggggag
      841 agtgtgatga taagtgggaa ggactatatt caaaggtgaa agcactcgtt aagtatatga
      901 aaaattctat agaaacttct ctcaattagg ctaatt
//
LOCUS       DAZULY010000397          915 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.410, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000397 DAZULY010000000
VERSION     DAZULY010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.410"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..>915)
                     /locus_tag="ACG81A_002867"
     CDS             complement(109..>915)
                     /locus_tag="ACG81A_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFS5016140.1"
                     /translation="TYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGL
                     QLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDL
                     TYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDR
                     GKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLR
                     LELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          245 a          212 c          177 g          281 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccgattcggc gccgactgag
      781 ttgaagccca cgctttgcta aacacttctt caattttcgg gtaccgtaag cctttcggtt
      841 tcgaataaac tcttcctgaa cgatctcttc taagtcggac tcattttcga tcggtttcgc
      901 ttgataatag taggt
//
LOCUS       DAZULY010000398          904 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.411, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000398 DAZULY010000000
VERSION     DAZULY010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.411"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            265..888
                     /locus_tag="ACG81A_002868"
     CDS             265..888
                     /locus_tag="ACG81A_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS5016141.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          333 a          129 c          167 g          275 t
ORIGIN      
        1 tagttttaaa ataaactaat gtccttattt ttgtgttcac atttctgtta cctaagaaat
       61 acttaaagga ggtgatgtag ccagcaacga ctacaacttg aacccgctat ctacagttta
      121 tttgttgttt cttttgggta tactctaatg gtacaaatcc atatttgctt aaagcgttga
      181 tttttcagcg gtttttcttt attgcaaagt gtaccctaag acctgtatac taatagtaaa
      241 tagattggat aggaatgatg gcaaatgtat ttagcttatt taagagtttc ttccgacgat
      301 caaagtttag ctcggcaaca taagctaatt gaaaattggt cagtcaaaaa taatattgct
      361 gaagacgatt taaaaatttt cgaggaaaaa gtctctggaa aaaatattga aaacagacca
      421 gagctgcaag agcttatttc tttcatacgt gaacgagata cgattattat tcctagtctt
      481 gatcgattag caagaaatag taaagataca aaagacttac ttcaattgtt tcgtagtaaa
      541 ggtgcttcta ttgaaatatt agacctaccg tcgtttaatg gggtaactga ccctgcatta
      601 agagacttgc taacaaattt agtcatagaa gtgtttagct atgttgctga aaacgaacgg
      661 aaaaaaatca aagagcgtca aaaacaaggc attgaaattg caaagaaaaa tggtcgttat
      721 actggtaaaa agattcaata ccatgccaat gcaacaggaa aaaacaaatt gatttacgat
      781 gaaattgttg atggtctgaa gaaaaaagaa actatttcta aaatagcaaa gaacgcagga
      841 gttacaagaa aaaccgttta tcgagtaaaa agagatttag gaatgtaata tgttccagtt
      901 agaa
//
LOCUS       DAZULY010000399          898 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.412, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000399 DAZULY010000000
VERSION     DAZULY010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.412"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>898
                     /locus_tag="ACG81A_002869"
     CDS             <1..>898
                     /locus_tag="ACG81A_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFS5016142.1"
                     /translation="YISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANIFFLLI
                     LKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPLIATVV
                     GWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKEYGDNE
                     TAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIANVLNCA
                     SGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSYVIYVV
                     RKRAVGKDIFIKHYHIVVESWG"
BASE COUNT          283 a          114 c          174 g          327 t
ORIGIN      
        1 attatatatc ctatgtatgg aatcaatttc taatacaatt tgctaaaggg ttagaaaaag
       61 ttaaagctat gggcatagca ggtattatca gcacaattgt aacaataatt gccaatattt
      121 tcttcttgct tattctgaaa tggggattaa ttggtttttt cattgcaaat attttatcac
      181 agattatttc agctacttat ctgtcagtat tagtgagaat atgggaatat atcgattttg
      241 aggctaaaga tcctcagcta aagagagaaa tgctaaatta ctcgattcca ttaattgcaa
      301 ctgttgttgg atggtggatt aatagtacag ctgataggta tgtggtttcc tttatagtag
      361 gtgtctcggc gaatggctta ttgtctgtgg catataaaat acctcaaata attaatactt
      421 tgcagggaat atttatacag gcctggcaaa tttctgcaat taaagaatat ggagataatg
      481 agactgcaaa attttatgga aatacatttg ttatgattaa tttgttaatg tgcataacct
      541 gttcaagtct tatcatatta actagaccgt tttcacatat tctttatgcg aaagactttt
      601 atgaagcgtg gaaatatgtt ccatttttat taatagctaa tgttttaaat tgtgcatcag
      661 gtctgttagg acctatctta tcagcaaaaa aagattctaa atccatgatg tggtctgcaa
      721 tcataggggc attagttaat gtgattatga gtgtagtcct tgtttataag ataggggttc
      781 aaggtgccac aatagccact gttatatgtt catatgtaat atatgttgtt cgtaaacgag
      841 ctgtgggaaa agatattttt ataaagcatt atcatattgt tgtggaatct tggggatt
//
LOCUS       DAZULY010000400          898 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.413, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000400 DAZULY010000000
VERSION     DAZULY010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.413"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..>898
                     /gene="msrA"
                     /locus_tag="ACG81A_002870"
     CDS             72..>898
                     /gene="msrA"
                     /locus_tag="ACG81A_002870"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS5016143.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNH"
BASE COUNT          338 a          154 c          174 g          232 t
ORIGIN      
        1 ttatttcctc actttatgat aaattacagc taaagtcact agcttgtctt cttcggaaag
       61 gaggagaatc tatggatgag gaacaaatat tcattcaaac actttataat ctgattttga
      121 atccgaatac acgagattgg gaacgaaaag tattgattca gacaaagaat gacataagag
      181 aaaatatatc cgttaaagaa cagttatcaa aactagaagc aacactacgt ccattagcta
      241 tccgaatgaa tctgacgcct gatgtaatgg acttttattt attgattacg gaaggatttg
      301 ataaggagca aaaatatgac ttttccaagc atgccatgca agatgctgac tatcaagaac
      361 gtgcagtatt tgcaggggga tgtttctggt gtatggtcga gccgtttgaa tcgaaaaaag
      421 ggatcctatc tgttttgtca ggatacacgg gcggacatgt tgaaaaacca aattatgatc
      481 aagtaagtgg tggttacaca ggtcatgtgg aagctgtgga gatcatttat gacacaagag
      541 aaataagtta tagcgagttg ctgacaatct attggcaaat tactgatccc acagatacat
      601 ttggacaatt tcaagataga gggaagcaat atcgtccagt gattttttat caagtcgaac
      661 gacaaaaaga attagcggaa caatcaaaac agaaactaga cagttcagga actttccacc
      721 aaccaatcgt cacaaaaatc gaaccagccg gtacgttttg gccagcagaa aactatcatc
      781 agcaatttta caaaaaacaa ccgaaacgtt ataaaaaaat ccagcaagca cgtaatcaat
      841 ttctgatcta tcaacgagta aaaaacaaat ggcaaaaaaa tatacgaaaa aatcattt
//
LOCUS       DAZULY010000401          894 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.414, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000401 DAZULY010000000
VERSION     DAZULY010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.414"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(71..301)
                     /locus_tag="ACG81A_002871"
     CDS             complement(71..301)
                     /locus_tag="ACG81A_002871"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFS5016144.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(395..733)
                     /locus_tag="ACG81A_002872"
     CDS             complement(395..733)
                     /locus_tag="ACG81A_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016145.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
BASE COUNT          284 a          184 c          102 g          324 t
ORIGIN      
        1 tattaacagc aaagatatcg aaaaaacaat aacataatat gtgtcaaaat ttttcataat
       61 aagaactccc ttactgttgc atactaacat aatccaaaaa ctcttctact tcagctaaag
      121 acattccact ttctttagtc caaaaaacag ttttttctat cgatccttct ctgtaaactg
      181 taaatgtcgg agtcgagtta attgagtagc gactcttgaa ttctttccac tcattttcat
      241 cttctctaac ttttgctaca ttcacgtaat aaatattttc ttttcccttt tctaccagca
      301 tatcgaatag cttcctgctt ttgatgatcg atttcttgtg ctacatctag taaattttca
      361 tcaatttcta tatcataaag tatttcacta gtgtctacat ccacaatata tactttttat
      421 cagtagcaat ttttgagtaa tccgttaaaa tgatctcagt ctcgtcactt tcatcagtat
      481 aaatatacgt taacaatgaa ctcacctttt caatcggtgt tagctctaag cctactgtat
      541 tttttactat tgagtcatta tttaccacca taacttcgta ctgtatatac catcctattt
      601 ggtttccatc aatatcagta attggctcac tagctaatgg agtctccgga acaatatttt
      661 cctgatattt cttaaactct tctgtagcac gaaaaaggtt atcatgttcc tcacttgaaa
      721 ctggttgagt catagaaaat gttacttggc taaacagagg gaaaataaac actacaatca
      781 acaaagtata aaaaaaagcc cttctttttc attttttatc ttccaatcac aaattgaaaa
      841 acttattcct gcaactttga gtctagtatc acaatcgact gactacaact attc
//
LOCUS       DAZULY010000402          885 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.415, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000402 DAZULY010000000
VERSION     DAZULY010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.415"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>885
                     /locus_tag="ACG81A_002873"
     CDS             <1..>885
                     /locus_tag="ACG81A_002873"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFS5016146.1"
                     /translation="SNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDG
                     TRFSQEYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGII
                     GAGYIGVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSES
                     PKKYLGRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQ
                     TNIKDIYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNG
                     FSIFNYNFYAAGLNLKTAR"
BASE COUNT          343 a          109 c          153 g          280 t
ORIGIN      
        1 aagtaattta gaagatcttt tttatcaaaa tagaatgaca ctaaattctg agataaaaaa
       61 gttatttcta gagacagaag ttgacaaaat tgatattcaa aataaaaaga ttagttgtgt
      121 aaaaaaagat ggtacaagat tctctcaaga atatgacaaa gttatattag ctacaggttc
      181 aaagcaaatt atgctagata taccggggaa taatttagct aatatttttt cgataaagaa
      241 ttttccaaat gcaaaagaga tttttaaagc tttggatgat agtactatta aaaaagtagg
      301 aattattgga gcaggatata ttggagttga aattgctgaa gccataagaa aaagagggaa
      361 agaagtatat cttttcgatg ttgcagatag agtgttgtcg acttattacg accgtagttt
      421 ttcagataaa gttgaagaaa tactatcaaa aaatggaata catctttgtc tatcagaaag
      481 tcctaaaaaa tatttaggaa gaaaaaagat tgaaaaaata gtcactacaa gtggtaccca
      541 atactctatg gatatggtcg tacaagctat aggatttcta tctaatagtc caattgggga
      601 aaaggaactc ttgagagatg ttagtggagc atatctaact aatgaatacc agcaaacaaa
      661 tataaaagat atatatgcta ttggggattg tgctacaact ttttttacgt ccattaatag
      721 aagtagtgtt gatttttcta tttctaatgc tctaagaaca gcttatattg caactcaaca
      781 tattaacaat caagatttta caccttctgg ttcccaattt acaaatggct tttctatatt
      841 taattacaat ttttacgcag caggtttgaa tttaaaaaca gcacg
//
LOCUS       DAZULY010000403          842 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.417, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000403 DAZULY010000000
VERSION     DAZULY010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.417"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..385
                     /locus_tag="ACG81A_002874"
     CDS             2..385
                     /locus_tag="ACG81A_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016147.1"
                     /translation="MSEDVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVVSE
                     IEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYA
                     VVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            399..800
                     /locus_tag="ACG81A_002875"
     CDS             399..800
                     /locus_tag="ACG81A_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016148.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
BASE COUNT          307 a          112 c          151 g          272 t
ORIGIN      
        1 aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata aagtaattaa
       61 tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt ctgtaacagc
      121 tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg aagttttaaa
      181 caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc ctcctcaagt
      241 taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag caggagagca
      301 ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg atatcaaggt
      361 caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata gtaccatcaa
      421 atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc gcatcggtaa
      481 ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa aatgataatc
      541 aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat ggcaaaattt
      601 ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa tatgatgaag
      661 atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc tcagattacg
      721 atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg aaatctgtag
      781 tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat ttccaaacaa
      841 at
//
LOCUS       DAZULY010000404          836 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.418, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000404 DAZULY010000000
VERSION     DAZULY010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.418"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..252)
                     /locus_tag="ACG81A_002876"
     CDS             complement(<1..252)
                     /locus_tag="ACG81A_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016149.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFN"
BASE COUNT          277 a          149 c           91 g          319 t
ORIGIN      
        1 attaaaattt ggtattgatg accagcgatt ccgtatataa tgcgcaatca aattaagctt
       61 tctgtctctg gtaaatattc ctgttctgtt ttcttgtact cgtaagattt cttcacttat
      121 cgcaaaattc ataaaattcc acactttttc ataaacaaaa tgtacacatt tatctattac
      181 ttcataatag tctacttgat attcttctgt aaacatggtt gctactgtat ctgcaccata
      241 tttgataaac atttgggcta ctcccttaaa ttgagaaaag cccattagtt tatctactaa
      301 tacttctaaa atttcttgct ttcttcttta acatcgtccc gtaaggaatc atgtagtatg
      361 ctggaggaag ttcattttct aaataattgt atcgcttcaa ttgctcaaag gctattctct
      421 acaattaatt acaccaatta ctttatcttc acttatataa gatttctaaa catttttcgt
      481 caaatgaaga gaaaatattt ttttaaatga tcttgctcaa gatttgtcaa cgaatctttg
      541 aattcgtttt gaagttctga ggaaggtatc atttaaaaac tcaatctgac tttcatcatt
      601 tcgtttactc ccattttgat caccttctac caacaattta ttaaagtgat tatactaggt
      661 ttttattttt ttaattcaca attgtttatt cacttcatga acaattaaac acaaaatcag
      721 tattaggatt aaactttcac ccattaatac actaacgaaa aaattttcat tttttaaaat
      781 agctaaacta taaatctcta tactgaaaaa aacaataccc atcaaaaata ctggga
//
LOCUS       DAZULY010000405          805 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.419, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000405 DAZULY010000000
VERSION     DAZULY010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.419"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(90..>805)
                     /locus_tag="ACG81A_002877"
     CDS             complement(90..>805)
                     /locus_tag="ACG81A_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS5016150.1"
                     /translation="FTEINLLNEYYDEIIEVAKKKDRLLYLSSTFLKRKEIQYIKSKV
                     THNKKISYQYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFAIELPWIIDTFGKVKS
                     FTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWE
                     GNPDSLFSWNEETSSMTDIQLYDEVMHDNNYSHTIIEDAYLEEIKEFFTLLEKKMEPQ
                     YTFEKDKYIINLINDIEVN"
BASE COUNT          271 a          137 c           88 g          309 t
ORIGIN      
        1 attaatagaa ttcttttttc catagcttat tagatttttt agatgtctct ttccaattga
       61 ccctaaccca aaaaaaatta ctttcatcat taatttacct caatgtcgtt aattaagtta
      121 ataatatact tatctttttc aaaagtatat tggggttcca ttttcttttc caaaagcgtg
      181 aaaaattctt taatctcctc taaatatgca tcttcaataa tagtatgtga ataattatta
      241 tcatgcataa cctcatcgta caattgaata tctgtcatac tagaagtttc ttcattccat
      301 gaaaatagag aatccgggtt accttcccat tctattaaaa tcttttcacc aattatagtt
      361 aaatttcttt tagctactct tgaaacaaca tctacactta aagttccaac tataccagtt
      421 tcatgctcaa taataagagt gaaactgtcc gaataatcaa tatcaagatt ggataatttc
      481 tgggtcatta cagtaaacga tttaactttc ccaaatgtat ctataatcca aggtaattca
      541 atagcgaata tttctctaca cccattagtt tttttgtttg aaacaaaaaa attgcgataa
      601 tcttcccaag gatgccagtc gggtaaatac tgtcctacat ggtattgata agaaattttt
      661 ttattatgcg tcactttaga cttaatatat tgaatttctt ttctcttcaa aaatgtagaa
      721 gatagataaa gtaatctatc tttcttttta gctacttcga ttatttcatc ataatattca
      781 tttaataaat tgatttccgt aaaga
//
LOCUS       DAZULY010000406          799 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.420, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000406 DAZULY010000000
VERSION     DAZULY010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.420"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            40..708
                     /locus_tag="ACG81A_002878"
     CDS             40..708
                     /locus_tag="ACG81A_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFS5016151.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
BASE COUNT          228 a          131 c          138 g          302 t
ORIGIN      
        1 agaagttgct gaaaattttc aaaagaaaag aggctagaaa tgaaaaaatt aaatttattt
       61 acagaattgt gcggaatcat tttaggcgca ggaatctacg gattagctgt gacaggaatc
      121 aatattccat ctaagctggc agatggtggg gttactggta ttgctttgct tttgcataat
      181 ctttttggat tcgctccttc tgtcacttca ctgcttttta atttaccttt attgcttatc
      241 tcattgtttc tttttggaaa acacgctttt ctccggacga tcgtcggaac atttgcgcta
      301 gttttctttt tacatttatg ggagagcctt catatacatt ttgctgtaga tagtctactg
      361 atcaatagtt tgatgacagg tattctttct ggcattggtt gcggtctcgt ctttcgattc
      421 ggcggaagca caggcggaac agatatcatt tatcaagcca ttgaaaaata ttttcatatt
      481 aagattggca aaagtctttt cttgattact tttggtattt tagttgtctc cttgctttat
      541 ttggatctca cacattttct gtatacatta cttagctgtt ctattctatc ttatacattg
      601 aataaagtga agtatctgga aattggatct gttttgcccc aattggaagc aaaacagact
      661 acagaaacat ttgaaccact tgatcctctt gaacagttag agcactgatt cttatttttc
      721 actactttat ttaatttaca gaaatatcat tatgtaaact aaaatgatat tattcagtaa
      781 aatcttcttt ataaaaaaa
//
LOCUS       DAZULY010000407          797 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.421, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000407 DAZULY010000000
VERSION     DAZULY010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.421"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..677)
                     /gene="gpmA"
                     /locus_tag="ACG81A_002879"
     CDS             complement(<1..677)
                     /gene="gpmA"
                     /locus_tag="ACG81A_002879"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS5016152.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKK"
BASE COUNT          215 a          148 c          150 g          284 t
ORIGIN      
        1 tattttttag caactgttaa gtcatcattt aattcataaa caagtggttg acctgttgga
       61 atttcaagat ccatgatgtc ttcgtcagag ataccttcga tatgtttagc tagggcgcgt
      121 aatgagttac catgtgcagc tactaaaaca gttttgttgt ctagtaatgc tggagcaatt
      181 tcatcttgcc agaatggcaa agcgcgttct aatgtcactt tcaagttttc tccaccagga
      241 atatcgcgtt tgtctaacat tgcgtaacga cggtcatttg ctgcagaacc ttcgtcagtt
      301 tcttccatta atggaggaag agtatcgtaa gaacgacgcc agatgtgcac ttgttcgtcg
      361 ccgtattttt cagctgtttc ttttttgttc aaaccttgta atttaccata gtgacgttcg
      421 tttaagcgcc atgatttgat ttgtggtacc caaagttgat ctgaatattc taagatcaag
      481 ttacatgttt tgattgcacg agttaaaaca gaagtgtaag caacatcaaa ttcgattcca
      541 gcttctttga ttttgcgtcc gccttctttt gcttcttcga ttccttctgg tgctagattt
      601 acatcagccc aaccagtaaa ttggttcaat gcattccatt cactaagacc gtgacgagaa
      661 aaaactaatt ttggcatgtt tagctctcct tttaagatag gtttatattt ctgaaatttt
      721 cagttctgtt ttattgtaca attttttgga agagatttcc atcgaaaaat tagataagat
      781 tctatgagaa aacaagt
//
LOCUS       DAZULY010000408          793 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.422, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000408 DAZULY010000000
VERSION     DAZULY010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.422"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(9..521)
                     /locus_tag="ACG81A_002880"
     CDS             complement(9..521)
                     /locus_tag="ACG81A_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016153.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            complement(528..>793)
                     /locus_tag="ACG81A_002881"
     CDS             complement(528..>793)
                     /locus_tag="ACG81A_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFS5016154.1"
                     /translation="NYYIQFKGFDFNSSTEFKLALYAQFFIEKLNRFQEIVEDSDFNT
                     FNSHKPMRGTYIKFLENYNDLKSTNNYQFGNLSYEMISKVLSL"
BASE COUNT          273 a          113 c           75 g          332 t
ORIGIN      
        1 taacatcctt aatatcttaa tttatccata ttttgagaaa taatactttt ccattcagaa
       61 actaatttag taaggaatct tgcaatgttt tgtctcatta attcattatt tctattatct
      121 ataagatctg ttagatattt ttcaaattct aaaaagctat ccgaattttc ttcgacgatt
      181 aaatacttaa gagaatttat atcggaaaag atagattcaa gtttactaat ctcattttct
      241 aaatctatat tttcacgtgt gattatctca tcactttttc ttaaaaagct aaaaattgca
      301 tcagttgctt tacacttttc cattaaagaa gtatattctt caatttttct tctttcttta
      361 tggtcatttt ttaaatcagt ttcaatgtct tttttcattt gttctatact gagtttgttc
      421 tttttgttct tttgagtaaa tctaaaattc aaacacgaaa aaagtatgtt agcaataaca
      481 cctataatag caaataaatc atttatacta tttaatttca tttatcatca caaacttaga
      541 actttactta tcatctcata agacaagttt ccaaattgat aattattggt agattttaaa
      601 tcattataat tttcgagaaa ttttatataa gtacctctca tcggtttatg gctattaaaa
      661 gtgttaaaat cactatcttc aactatttct tgaaacctat ttaatttttc aataaaaaat
      721 tgtgcataaa gtgctaactt aaattcggta gagctattaa agtcaaaacc tttaaattgt
      781 atataataat tat
//
LOCUS       DAZULY010000409          784 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.423, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000409 DAZULY010000000
VERSION     DAZULY010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.423"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="ACG81A_002882"
     CDS             complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="ACG81A_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS5016155.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          206 a          174 c          123 g          281 t
ORIGIN      
        1 ggtacacggt gttccaaaag tagcttcttc ggaaataagt tggaatttca caaaaattca
       61 agaagtaatt ttcgtaaatt ccttcttatt tcttgaagct gattacttct gtcacaacct
      121 ctttttatta ttgagaattt ggtcgaggaa taatcgtttt tgccatccaa atatccggtt
      181 tgtcccagcc atttgcattt ggtaagacac ctacgatttt ataacctaat ttttcataga
      241 attcatacgg atgttcacga aggttctgga tggaagccac tttatcaaat gtatgctcat
      301 acaggtccgt ttgacttaac gttgttccat ggtctaaatc atccgtacct aaataaatcg
      361 tgattcctcc tctggaagct acttcttttt ctaagtaatt gactaatcga gtacctattt
      421 ggttctttcg tcgggagctt tctacaacta atggatgcaa ttcccaacct gtgataccgt
      481 attgagggat tgcaccaata aatcctacta actcatcttg gtctaccgct gctaccgcga
      541 ttcgttctgg attcatcatt tcttctactt cttctgccga gctgtctcca tattcttccg
      601 gccaagtcag tctcagtaaa tcagaaagct gatctttcaa tactggatta ttacggtcaa
      661 attcactgat tatcatacgt cttccccctt gttcttgctt attctaacag tccttttgaa
      721 aaaacgaaat gttctcgctc aaaaatcttt agctaccaat cagtatctct attctttcat
      781 tatt
//
LOCUS       DAZULY010000410          768 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.425, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000410 DAZULY010000000
VERSION     DAZULY010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.425"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(97..495)
                     /locus_tag="ACG81A_002883"
     CDS             complement(97..495)
                     /locus_tag="ACG81A_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS5016156.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(492..728)
                     /locus_tag="ACG81A_002884"
     CDS             complement(492..728)
                     /locus_tag="ACG81A_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS5016157.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
BASE COUNT          239 a          134 c          119 g          276 t
ORIGIN      
        1 ttatgaaaaa actattttgt atgattaaac ataaaaaaat cagcatattc cttaatgaca
       61 tgctgatcta attgagaaat aaagttttta attcgttcat tttattgcgg tttgtcgtaa
      121 ataactggtt atcttggact ttagtgaatc gaaatccaat gaactagtgg taatcattat
      181 gataaaatca acagcttctt gacgagaaaa agcaactgga caattattca attgaagaaa
      241 caacaacatg gcaacaagag cagttcgttt attgccatta tgaaaaggat gttttttagc
      301 taaattaatt gctaaaatcg ccgctttttc atagatttca ggatagagtt cttgattgaa
      361 tactgcttgt gaaggctgtt tgacggccat atctaatgca tttgcatctt ttacaccgat
      421 catttcgcct ggcgagtgac gtaaaataac tttcgcgttg attttgataa tttctttagt
      481 cgaaagatac ctcataaatc aaccaaatct tttaacgcgt cttcatattg attcatgatc
      541 gaatcaacta atgaatcaat atcaggttct tcttgtgctt tgatcattcg tacagaacca
      601 tcagattgaa aaacataact gattgcgtca cctgtctgca cacccatgaa ttcttttact
      661 tctggtggga tagttgttac atcgctattg ccagattttc taattttata ctttttcatt
      721 tttttcatat ttcgccaccc tctataccat tctcattcta tagtaagt
//
LOCUS       DAZULY010000411          756 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.426, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000411 DAZULY010000000
VERSION     DAZULY010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.426"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            126..>756
                     /locus_tag="ACG81A_002885"
     CDS             126..>756
                     /locus_tag="ACG81A_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS5016158.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT          266 a          107 c          124 g          259 t
ORIGIN      
        1 agaacgtttc aagagatagc gacactatcg ttatcgaaaa aggcgaaaac ctatctcatt
       61 ttctgctaat gtaagttggt ttagcttttg ttaatatgac tttatgaaaa tggaggtaat
      121 taaatatgat ttttcctaaa gttaatgaag caagcttgaa taaaggatcc ttttttcata
      181 tctatgaaaa cgaagatttt gaactgtata atatcctttc acaaaaagag gtcatcgaaa
      241 accaaataat ctgccatcca attatttaca aatctatttt caagtatgca ttttttgctt
      301 ccaatgattt tcaaagaatg gagattaccg actgtatatt cgaaaactgt gatttttcag
      361 gctgtatatt gacaaaaagc atgttgcatc gcgtgaaatt tgtcaattgt aaattaacag
      421 gtactaaatt gatggaaagt tatattggga acacgttatt cgagaattgt aaaatggact
      481 atgtaaattt atctggttca aacataaaag aaagtacctt tgagcactct gtcttatcaa
      541 gcgcagattt tgtagactgt tccctcacta aaataatgtt ttctactaat gatttagaaa
      601 atgctagctt ttttgatact gatttaaaaa atatcgattt aagtagcaac tcctttgaaa
      661 tgattgaagt acgctcggaa catctaaaaa attgtacgct taatcagttg caagcacttg
      721 gttttgctag aaaattttta caaataaaag tagtat
//
LOCUS       DAZULY010000412          748 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.427, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000412 DAZULY010000000
VERSION     DAZULY010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.427"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..>748)
                     /locus_tag="ACG81A_002886"
     CDS             complement(26..>748)
                     /locus_tag="ACG81A_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFS5016159.1"
                     /translation="FLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGT
                     NYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAA
                     LKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSG
                     IIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRD
                     VISIVFWYYLGSISYLYLKKEN"
BASE COUNT          283 a          134 c           88 g          243 t
ORIGIN      
        1 ccaagcaatg aaagctgtct tcatattaat tctccttttt taaatacaag taagaaatgc
       61 ttcccaaata ataccagaaa acaattgaaa tgacatcgcg cttattgaaa agtacatgag
      121 cctccaccat ttcatctata aaaatagata gtataaaaat taaaacaata ccaagaaacg
      181 cataattcac tttacttcta aaaaatgttt tgagtaaact aaagatattt aacacataga
      241 aagcatagat acatataaaa gcaattatac cacttgatac aagtacttga agaaatccat
      301 tatgcatgtt tccatgtgct ctttttagtt cattcacatc tgtagtatca agatctactt
      361 ttttcaaaaa ttttgcatcg ctattcccat agaaatcggt ctctccataa ccaaataaca
      421 aagttggtct tataacttta agcgcagctt tccaaatcgt aaaacgacca gctgaaattt
      481 cggagttatc actagaatgc tgtaatatta cttctttttc tggaagtgga gttttatctg
      541 attcatttga ctctataact tctacttttc tatcaatcga tacaggaatt gttgctaaaa
      601 aatccttagc ataattagta ccaaaagaaa aatataccaa tagaactaat aatatgctta
      661 ccatccattt gatttttaca ggtagtactt ctctaatgct ctttctaaat tttgaattgg
      721 caaacaacaa aagaaataat actaaaaa
//
LOCUS       DAZULY010000413          735 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.428, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000413 DAZULY010000000
VERSION     DAZULY010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.428"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..382
                     /locus_tag="ACG81A_002887"
     CDS             <1..382
                     /locus_tag="ACG81A_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 32"
                     /protein_id="HFS5016160.1"
                     /translation="LFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGK
                     TPNYPWPQMFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTAL
                     SMTVTEGAIKVDKLDYSINYECGF"
     gene            405..>735
                     /locus_tag="ACG81A_002888"
     CDS             405..>735
                     /locus_tag="ACG81A_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS5016161.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFY"
BASE COUNT          266 a           84 c          148 g          237 t
ORIGIN      
        1 tctatttgaa acaactttga ctattacatc gttaacaaag gagtttgctt taaaattatt
       61 tgtagatagt gatactcaag aaggatacga atttcgtatt tcattgatta ataatcagct
      121 tactttcgga aaaactccga actatccttg gcctcaaatg tttgataagg ggctgcaacg
      181 accactattt ttggaaatag gcaaagaata tgaactaaag ttaattgttg atgatacgat
      241 agctgtttta tatattgata atgttgcttt aagtactaga atgtatgata aagtcggaac
      301 agcattaagt atgactgtta cggagggagc aatcaaggta gataaactag actattcaat
      361 aaattatgaa tgtggatttt aaaacaattg aagggatgat gtaagtgaaa ttaaatggtt
      421 tacaacatat tggaattcca acaaaagatt ttggagcttc aaaaatcttt tatgaatcct
      481 tagggtttag tctgattaat gaagaaatga atgtgggaag taaagtcggc tttttcgagt
      541 tgaatggaac gataatagaa atatgggaag ataaaactac tgagtgtaga ggagccattg
      601 atcatctagc attagatacg gatgacatag atgaggtatt caattcaatt caagatttag
      661 gatatgaatt attagataaa aatattatgg aattaccgtt ctgggaaaat ggaatccgtt
      721 actttaattt ctacg
//
LOCUS       DAZULY010000414          733 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.429, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000414 DAZULY010000000
VERSION     DAZULY010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.429"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>733
                     /locus_tag="ACG81A_002889"
     rRNA            <1..>733
                     /locus_tag="ACG81A_002889"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DAZULY010000415          725 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.430, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000415 DAZULY010000000
VERSION     DAZULY010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.430"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>725
                     /locus_tag="ACG81A_002890"
     CDS             <1..>725
                     /locus_tag="ACG81A_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFS5016162.1"
                     /translation="SFISTFLNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIY
                     LRIMIKGAHFFDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSY
                     ISNIGIILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSI
                     DLAKFMAPKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVN
                     ILLNMIFIPRLGIIAAGYTTVFT"
BASE COUNT          225 a           94 c          135 g          271 t
ORIGIN      
        1 atcttttata tctacttttt tgaatattgc aatctctgta gctttgatgc agacattata
       61 ttttgatgat aaatatgttg gcagagtagt cggcggaaca ctgtcatttg tgttgatagg
      121 tttagtaatt tatttaagaa taatgataaa gggtgctcat ttttttgata aaaaacagtg
      181 gagatacgct ttaaaaatct ctttgccgct aatcccacat tccttagcaa atatagtttt
      241 agcacaattc gatagaatta tgattaattc ttatttaggt cctgctgcgg ggggaatata
      301 ttcctacatt agtaacatag gtattattct aagcgtaata tggatgtcta ctaataacgc
      361 ctgggttcca tggttctatg gtgagatgag taaaaataat gaaaaaaaag tgaagaaagt
      421 tagtaactat tatttagtag tctttacttt gattacgatg attgtaatgg tagtttcgat
      481 tgatttagca aaatttatgg ctccgaaaga ctattattct gggttatctt tagtggttcc
      541 gataacctta ggctatttct ttcagttttt atatagttta ccagttaatg ctgaatttta
      601 tgagaaaaaa acatcgttta ttgcgattgg tacagtagca agtgctatag ttaatatact
      661 attaaacatg attttcatac ctaggttagg aataattgct gctggataca caaccgtatt
      721 tacgt
//
LOCUS       DAZULY010000416          723 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.431, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000416 DAZULY010000000
VERSION     DAZULY010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.431"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            146..382
                     /locus_tag="ACG81A_002891"
     CDS             146..382
                     /locus_tag="ACG81A_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFS5016163.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            401..616
                     /locus_tag="ACG81A_002892"
     CDS             401..616
                     /locus_tag="ACG81A_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFS5016164.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
BASE COUNT          233 a           98 c          178 g          214 t
ORIGIN      
        1 aacactaact ttttcattcc tgaatcaaat ttattttgga aattttacta ttcaagaata
       61 aacttgataa gtttcaggaa gatttttact gttaatcaaa tacgtatgtt acagtagcac
      121 cgtaaaaaat attatttaaa gaagcgtgtt taaacatgca aaatgtaaag gaactaagta
      181 ttgtggaaat gcacaaactg ttggtggagg caataatgct tggggaaaat tggggcaagt
      241 tgtaggtgga cttaccacag gggcagtagg tggtgcaggt ttaggaacag caatttgcgg
      301 accagcttgc ggtgttgttg gagggttata tggagctgtc gctggaggtg ctgcagctgg
      361 ctgggatgct cgaaaaaaat aaaaaaggag aataagtagt atgcatatta aaaacacaaa
      421 aacaacattt atattatctt cagaagagtt gaaaaatatt caaggtggga gtgccgtggg
      481 agttttaggc actacttttt ctggggcaac agcaggagta aaactttgtt cagccggagg
      541 accttatgca atagctgctt gtggtgtagg tggagcttta ttaggtgctg gattcagcat
      601 gtggaccggt gcgtaaacaa ttaaaaaata tcaaacctca agattggttt gtgtcgatta
      661 cgaacatagg tatgttcttt atcttttcaa aagtttggaa tggacagaac tttttactaa
      721 agc
//
LOCUS       DAZULY010000417          719 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.432, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000417 DAZULY010000000
VERSION     DAZULY010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.432"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            322..>719
                     /locus_tag="ACG81A_002893"
     CDS             322..>719
                     /locus_tag="ACG81A_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFS5016165.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWV"
BASE COUNT          235 a          137 c          116 g          231 t
ORIGIN      
        1 atagttgtta ggtgattcat ctagtaaaaa caccagattt caaatttcac acaatttaaa
       61 ctagatcaat catcaacctt cttcaaattg ttagaaccct ttcccgagga gctgcagcat
      121 aagcaaaaaa acaaacagga agccaaatgg ctaagacatt ttttctatta gtctagttgt
      181 ttctcgtcgc aagtttaaga ctttagaaag ttatttctaa tgatgtttgg gttgttttga
      241 tcaatgctta tgcacagctc cttacctatt atttatctca acagatctaa caaattaatg
      301 gactatttaa ggagtttttt tatgcttacg atctattctt ctcgcacaca tagttaccat
      361 ctgatggcaa actggctgaa agaccatcaa atgccttaca gagaacgaat tttcacacct
      421 aaacagcctt taacaaggaa agagatattt tatttattgt cattaacaga aaacggattt
      481 gacgatctat tagctaaacg ctcgattgat tataaaaatt tgaaagacca aatcgaccaa
      541 tgtacgacct cagagcttgt cgatctgatc gtctcgcatt ttactttgtt acaacgtccc
      601 atcattactg acggaaagaa attattagtc ggattcaatg aagatagcat gcgtcttttt
      661 atccctgtac aatggcgcag aagtcaactg aaatctgtct ataacgattt tacatgggt
//
LOCUS       DAZULY010000418          712 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.433, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000418 DAZULY010000000
VERSION     DAZULY010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.433"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            98..>712
                     /locus_tag="ACG81A_002894"
     CDS             98..>712
                     /locus_tag="ACG81A_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS5016166.1"
                     /translation="MKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRTPI
                     REALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQKK
                     QVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERFRW
                     LRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLD"
BASE COUNT          275 a          106 c          122 g          209 t
ORIGIN      
        1 agctagtatg ttactttgaa atttgatatt atataacgag catagaacta aaatatcaaa
       61 ttcaaaaatg aaattttgag agggattaaa tatgacaatt aaaactagtc tccaaaatca
      121 agcttatcaa tctattcgtc agcaaattat ctacgctgac ttagaacctg gaaaaaaagt
      181 atcagaaaaa aaattggaag aaacgctttc tatcggtcgt actccaatac gtgaagcatt
      241 gattcaactc cgtcatcaag gacttgttga tacaattcct caaagtggaa attatatctc
      301 attaatcgac ttagatttag caaaaaatgc tcgttttgtc agagaacatt tagaacgaca
      361 aattatgcta gagtgttgtg ccaagatgaa taatcaaaag aaacaagttt tagaaactat
      421 aattgcagaa caagaaaaag ctgttagtca aaagaatgct cgtagttttt ttcataatga
      481 taatttattt cataaagctt gttttgaaat tgcagaacgg gaagaagtat ggcaatggtt
      541 ggaagataat aatacgcact tggaacgatt tcgttggtta cgagtaaaaa cggaaggatt
      601 aaaatgggaa aaaattatgg aacaacatca tcaactattt aaagccttga ccatgcaaga
      661 ccctgaagaa gctaacttct taacatcctt gcatcttcat ttaatgcttg at
//
LOCUS       DAZULY010000419          706 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.434, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000419 DAZULY010000000
VERSION     DAZULY010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.434"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>706
                     /locus_tag="ACG81A_002895"
     CDS             <1..>706
                     /locus_tag="ACG81A_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016167.1"
                     /translation="DTAFHINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPL
                     FLLIKFTNLAIGYNLFLYIVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVE
                     IFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVM
                     IIIALIIQFYRKKKIEIFLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDIL
                     QKRAIYLGKEFLIGSID"
BASE COUNT          221 a          101 c          106 g          278 t
ORIGIN      
        1 gatacggcat ttcacattaa tcgtgcgtta agtttagaat ctatttttag ctcccctatt
       61 aattttgaaa cgtttagatc atatggcatg caagtaaata atttttaccc atggttaact
      121 ctatatcctt tatttttatt gatcaagttt actaatttag caataggcta taaccttttt
      181 ctttatattg ttacgctcat tactctattt atatgtcatt atgtaatgta tgaaattacc
      241 aaaaaacatg ttactagctc ctttttcgct attatatata caacatcttc ttttaggagt
      301 gtagaaatat ttcttcgagg ggcaatgggg gaattactcg caatgtcaat acttcctctt
      361 attttactcg ggtttataaa gctttacgac tcaaaaaaag aatcttgggt tatgttggcg
      421 attagcatga ctttattaat ttacactcat gttctttctg tagctatgac tatggtaatg
      481 attataattg ctttaattat tcaattttat cggaaaaaga aaatagaaat cttcttaatt
      541 attaaattaa taaaagctgc tatagttact ttacttcttt cactagcgtt tataggtcct
      601 atgattgagc agacattgta tcaagatctg aatagacctt atgtggatat attacaaaaa
      661 agagcaattt atctgggtaa agagtttttg attggaagta tagatt
//
LOCUS       DAZULY010000420          700 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.435, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000420 DAZULY010000000
VERSION     DAZULY010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.435"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..>700
                     /locus_tag="ACG81A_002896"
     CDS             2..>700
                     /locus_tag="ACG81A_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFS5016168.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGI"
BASE COUNT          243 a          100 c          129 g          228 t
ORIGIN      
        1 tatgaacgat tttactacag aaattatgga aatactaatc aataaaggtg atttggatga
       61 tttgtttcgt cgtcacctag aattggctat taacgcttta ttacaagctg aattaacggt
      121 atttcttgac tatgaaaagt atgatagagc tggatttaat tcaggtaatt cccgtaatgg
      181 gaattattcg cgctctttta aaacagagta cggtgaattg aatttagtaa tacctagaga
      241 taggaatgga gacttttctc aacaaacttt acctgcttat aaaagaacca atgactcttt
      301 agaaaccact attattcagc tagttcaaaa aggaattact atgtccgaaa tctctgatct
      361 aatcgaaaaa atgtatggtc attattacaa accacaaact atttcaaata tgagtaaaat
      421 tgtgtctgaa gatgtttcgg catttaaaga aagaacttta gaagctaaat actcagtcat
      481 ttttatggat gctactcata ttcccttaaa aagacaaacc gtatcaaaag aagctgtcta
      541 tatagtaatt ggtatcaggt tggatggaac taaagaggtt ctaggattta ctatagctcc
      601 aactgaatct gcttatgttt ggaaagagat tcttcaagat ctaaaagacc gtggtttaga
      661 agaggttttg ttagtcgtaa cagatggttt aagtggtatt
//
LOCUS       DAZULY010000421          694 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.436, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000421 DAZULY010000000
VERSION     DAZULY010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.436"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..457
                     /locus_tag="ACG81A_002897"
     CDS             89..457
                     /locus_tag="ACG81A_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFS5016169.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT          245 a          115 c          105 g          229 t
ORIGIN      
        1 aacattactt gtgttttgtt cgttttttat acataactat tgtttttctg gtaaaataaa
       61 tcaaaccact taatggaggc gttttagaat ggaaaaattt cagccgatca tcaatcaaat
      121 caaagaaaaa gaacatcaac aaaagatcaa agaccttttt gctaagataa cagaaacgta
      181 tccccagcta gacacaactg taaaatggaa ccaacctatg tttactgacc atggtacctt
      241 tattattgca tttagttttg ctaaaaatca tttttctatc gcaccggaaa aagcagcgat
      301 tcgtgccctt gaaaaaaata tccaagaagc aggctacgta tatacggata acgtcatcaa
      361 agttccttgg aaaagtgtaa tcaactggga attgattgaa caattgattt catttaatat
      421 tgaagataaa aaaggtcatg ataaattttg gcgataaagc tttatacgaa taaacctttt
      481 actggcgagg aaactattca tcaccttata caggttttgt tcaatcctat cctttttatc
      541 atccctggtt aaacacttaa ttgacaaagt atctgctatt tttataagga gtctttcttg
      601 tgctgaaaaa aatcactgat tgaatggctt tgaattttct acatagtaaa attatttttt
      661 cagcaatttt cctgcaaaac ttttgaataa ggaa
//
LOCUS       DAZULY010000422          693 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.437, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000422 DAZULY010000000
VERSION     DAZULY010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.437"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACG81A_002898"
     CDS             132..344
                     /locus_tag="ACG81A_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016170.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>693
                     /locus_tag="ACG81A_002899"
     CDS             506..>693
                     /locus_tag="ACG81A_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFS5016171.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVL"
BASE COUNT          247 a          103 c          122 g          221 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct aga
//
LOCUS       DAZULY010000423          680 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.438, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000423 DAZULY010000000
VERSION     DAZULY010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.438"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          228 a          121 c           88 g          243 t
ORIGIN      
        1 actattcatt gatcataacg attggctatt tgattgtatc aaatctagga aatatggtat
       61 ttggtatcag taaagaattc agttggacta ctacattatg ggaatctcta ttctttttca
      121 tcttcgtatt tttgttacaa aattatcgaa aaaaataaac atgtgccatc tatctgttgg
      181 cacatgtttt aggatttatg gtacttttta ttcggcaatc cattttattt ttctacttct
      241 tcttaatacc tctaacactt ttgatttata aactaatcca atgcatctac ttgcgcttat
      301 taagctattt cagctagata gtaccacccc atgcgtcaat gcttgatttt ccttttgtat
      361 aacattgtat aagcaataaa ggacaaataa gtggtgatta caccaaacca ttgaataaga
      421 aaaattctga tacgtaaata aacatgaact tgaaaaaatt aatgaatcgg ttagctacta
      481 cgtagttgtc ttttttctca tacagttctg ttaacttaca cttctcttcg ctagataata
      541 aaaaatcctc caactgtatt gtccaatata atcccaggca tctaaactag cattatagaa
      601 gagacacaca tagaatctaa aataactggt tttaaatgtt tcttacaatc aaaaaaagct
      661 gttaaatgca ctccacacct
//
LOCUS       DAZULY010000424          680 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.439, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000424 DAZULY010000000
VERSION     DAZULY010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.439"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>680
                     /locus_tag="ACG81A_002900"
     CDS             <1..>680
                     /locus_tag="ACG81A_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HFS5016172.1"
                     /translation="LLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVM
                     HGKITASAIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVD
                     MGSLEQMYEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKV
                     QYYKGLSQKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTM
                     IFKNTICVL"
BASE COUNT          245 a           77 c          134 g          224 t
ORIGIN      
        1 ttattggcaa atgagatttg gagtagttta gaagtcgaag aaagtaaaca agaacttttg
       61 ttgaaatgga tactttttct tgtaaagaag ttctatgtga aaataccaga aacccattgt
      121 ctgatagtta tgcacggtaa aataacagct tctgcgatcg caagggaaac aaataagttg
      181 ctgaatacat atgtttatga ggctttcgat atgccaattg aaggtgagac tagtgactta
      241 attaacctaa tcaacaattt ttgtaaaagt atagatacga cgaatggact cattctgcta
      301 gttgatatgg gatcattgga acaaatgtat gaaaaaattg aatccaatgt aataggtgat
      361 ttggtaattt taaacaatgt ttctacagct ttagcgattg aatgtgggat tcaagtttgc
      421 caaaaaaagc caattagtca tttttaccag atggattttg attcgtttca agttaaagtt
      481 cagtattata aaggattatc acaaaagaaa aatgtaatag tatcttgtct ctctggtgag
      541 gggatttctg aaaaagttaa ggatatttta aaacggtatc tcgataatca agtggagatt
      601 ttgatttttg attttaacgc attaaagaaa atagcaaagg aaaaagatac tatgattttt
      661 aagaatacaa tttgtgttct
//
LOCUS       DAZULY010000425          678 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.440, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000425 DAZULY010000000
VERSION     DAZULY010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.440"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>678)
                     /locus_tag="ACG81A_002901"
     CDS             complement(<1..>678)
                     /locus_tag="ACG81A_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS5016173.1"
                     /translation="YDFSAQDIAMKFCIKRNVASHYLNQLFSDGKLLKNDSVRPVMFK
                     YNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLPLIIKGNSGVGKSFLASLIY
                     QYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKKGAFTGAEKDTAGMLSSANG
                     GYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEYANVRLILATTEDKNTSLLA
                     TFRRRIP"
BASE COUNT          215 a          126 c          105 g          232 t
ORIGIN      
        1 ctgggatgcg tcttctaaaa gttgctaaca aagaggtatt tttatcttct gttgtggcta
       61 agataaggcg aacatttgca tactcccatt gcacactttc accaagttta cgatactttt
      121 gagaatccat aagaagaaac aatttctctt ggttttctgc acttagattg tgaacctcat
      181 ctaaaaaaag gtatcctcca ttagcagaag atagcatccc tgcagtatct ttttcagccc
      241 cagtaaaggc tcctttttta tatccaaata aaactgctga caatagctct ggattatttg
      301 cataatctgc acaattcaca acaacaaatt ttgcgtcatt atgaattact tttctatcaa
      361 gagcatactg atagatcaaa cttgctaaaa aactttttcc aacgccactg tttcctttta
      421 ttattaatgg gaggccatta gggggataca ttacagtagc tttgcattta tctatagtgg
      481 atttcaaaga tatgtctgct ccaataaact tagaaaagca gtcttttggc ttttgttgat
      541 tatacttgaa cattactggt ctaacagaat cattctttag taattttcca tctgaaaata
      601 actgattcaa atagtgggaa gcgacatttc ttttaataca aaatttcatt gcaatatctt
      661 gagcagaaaa atcgtaat
//
LOCUS       DAZULY010000426          663 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.442, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000426 DAZULY010000000
VERSION     DAZULY010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.442"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..281)
                     /locus_tag="ACG81A_002902"
     CDS             complement(<1..281)
                     /locus_tag="ACG81A_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS5016174.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVI"
BASE COUNT          218 a          107 c          106 g          232 t
ORIGIN      
        1 ataatgacgt caggcgtcca accaagagtg actttctctt taatgtaatc tacttcatga
       61 gcaggtaatt gaatgacttt tctaccacat tttttcttat tttctttata ctggtgccaa
      121 taatcaagag cagtcttgcc ttgcttgaac ttattgacta cattataaat agtttgtata
      181 gcacgtccca ttcggttagc gatttcaacc ggcttattat gttgaagata atatgattct
      241 atcattgtca gttcgtctat ggtaagatgt ttgtaggtca tttatggtta cactcctttg
      301 ttttctttcg tcggaaatac aatttgagtg taccataaat gcctttttat ttttctaact
      361 taattttaca attcgcgata tattaaacaa cgttgtcgcg cttcattgaa acattttatg
      421 aaaattccaa aaaagagttg acacatgttt ttagacgtga taagataact aagtcgttaa
      481 aaagaaccac ttgcgaagct tgattctatc gaaaaaaaac ttcaaaaaag ttcttgacaa
      541 ataacaaacg ataagttatg ataaatgagt tgctgaaaga tttcgaaaaa cgaaatcgaa
      601 aaaaactttt aaaaaagagt tgacaatcgc ttgtctctct gatatgatat aaaagttgct
      661 acg
//
LOCUS       DAZULY010000427          659 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.443, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000427 DAZULY010000000
VERSION     DAZULY010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.443"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            25..>659
                     /locus_tag="ACG81A_002903"
     CDS             25..>659
                     /locus_tag="ACG81A_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFS5016175.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFT"
BASE COUNT          258 a           66 c          141 g          194 t
ORIGIN      
        1 taaatattag aggtataaaa agttatgaaa cacaaggtaa gtgtgatagt accaatttac
       61 aatgttgaaa aatatttaag aaaatgtgtg gatagtttat tgagtcagac attaaaagag
      121 atagaaatcg tattagttga tgatggttcg ccagatgctt cgggggaaat agcagatgag
      181 tatcaaaaaa aatattctaa tgtaaaaaca atacatagag aaaatggggg gttaggaccg
      241 gcaagaaata caggaattga gaatgcgaca ggagaatatg tggcttttct tgattcagat
      301 gattgggtag aaagtgatat gtatgaaaaa ttatacttag ttgcgtccaa agaaaatgct
      361 gatatagtag ttagtggaca ttgtgatttt gcgaatgaca aagctatggt gaaaaagcaa
      421 catcctttga gtgggaatgt atatgatacg cctaatagta ttaaaaatgt acgaaaaaat
      481 ttatttggac atagtccaaa agatcaagaa gtagaggcct tcccgatgtc agtatgtatg
      541 tctttgtata ataaaaatct gctagataaa tatagactaa gatttgaaaa aattttatct
      601 gaagatacaa tttttaactt gaatgcatat gatgtcgcaa ataaaattgc ttttacaga
//
LOCUS       DAZULY010000428          658 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.444, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000428 DAZULY010000000
VERSION     DAZULY010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.444"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(194..343)
                     /locus_tag="ACG81A_002904"
     CDS             complement(194..343)
                     /locus_tag="ACG81A_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016176.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT          215 a          107 c           98 g          238 t
ORIGIN      
        1 taacaaaatt gtcataaaaa acgagatggt tgatattaga acttgtgttt ttgtttaggt
       61 ctagagataa agagagacaa tttccaaata tatgtaatga atacaaaaag acgtttgtcg
      121 cggattaccg ttacaaacgt cttttattga ttttccgaga tctcatcagt cgccatttgg
      181 aatggttcac tccttatcta gattcgttta atgctcggac tgcttgttcg attgtaatac
      241 caaacgcaac gttattttcg ttttgtgcat caatcgccat aaaacggtta gaagttgtat
      301 caaaagctac gcggtattgc atatttgttt ttgtaaacgt cataaaattc atctcctttg
      361 taatgtaatt tagttcttga aagtttcctt taaactttcg aacttatacc aagtttagca
      421 tcgtacgttt tttgatgcaa ttaaaaagca tttatcattt tccttattta ttttttctta
      481 aatggtttag ctattttctt ttaatccgat aagtagccca atcatcaagg ttacaaatac
      541 tattgcttga atataaatca tatgctcacc tctttataaa taacttagca atataaagaa
      601 aaaaacgcac gaacaacata ggaatataaa caaacgtttt tgtcacaacg tctaagat
//
LOCUS       DAZULY010000429          645 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.445, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000429 DAZULY010000000
VERSION     DAZULY010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.445"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            175..438
                     /locus_tag="ACG81A_002905"
     CDS             175..438
                     /locus_tag="ACG81A_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS5016177.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            438..>645
                     /locus_tag="ACG81A_002906"
     CDS             438..>645
                     /locus_tag="ACG81A_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFS5016178.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVY"
BASE COUNT          248 a           80 c          129 g          188 t
ORIGIN      
        1 gatgaagtta taatcaaata tatgaagcct gtgataaaac ttgttaataa aaataaagga
       61 aacggataac actttacaat gctgttttgg tactccaatt taaacaaaac aatcgaattt
      121 acgtacttaa ttaagtacgt tagaatggag atataaatta gagggggagt agatatggca
      181 aaaacagtct taaatcctag ctcagcacgg aaaaattttt ataagttgtt gaaagatgta
      241 aatgagaatc atacggaaat tgaaatcatt agtgagcgca gtgaaaataa tgcggtgctt
      301 attggtttgg atgattggaa agcaatcaaa gaaacgctat tacttgagca aataggcact
      361 ttgggtgttg ttcgtaagcg agaaaaagat gatagtggat ttactaatgt tgatgaaatt
      421 gattgggata acttataatg aataactatt ctgtgatgat taaaaactcg gctaaagctg
      481 atttaaagaa aatcaaacag tctaatttga aacctcaatt tgagaaggtt attcagactt
      541 taaaggaaga tccctatctc cctacccaat catttgagaa gttaaaacct acacatgaag
      601 gtaggtattc tagaagattg aatagacaac accgggttgt ttata
//
LOCUS       DAZULY010000430          636 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.446, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000430 DAZULY010000000
VERSION     DAZULY010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.446"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          228 a           78 c          116 g          214 t
ORIGIN      
        1 atttatgaag tttttagatt ttttggctaa gtaaatgcat ttgttcactt gacatgtcct
       61 aactaattat caagcaagga acattttctt cgttagtaat ggattttaaa gaaatttcag
      121 tttttggatg ttgaataaaa gaccatttgt gaaaccacta gtatacatac tcattaccat
      181 gtcaattcta ttactttaaa agaaaatcgt tttactttga agaggagcat atctatccat
      241 taaaagatga tggtaatgca atcactagtt cgagtcattt taagagccat gcagtgagct
      301 ggaaataaaa cgaatagaag atgggtttaa caaaaggacc agtcatagga agaaattgtc
      361 gtgaaatcga atgataatct gaacggtagg caaagactgt taaaaagatg ggaaaggtat
      421 gtgcgatcat tttactgcac gattatttgg aaaatacctt gttgttcgct ttttctggaa
      481 aaaatacata aaaaaagata aaacgattgc gaactgcgtg tattcggtct ttcgatcttt
      541 ttcacggtat ggtatgatag ttaagaattt tgagaaattt taaaaaataa taatcgttac
      601 acaaaaatgt tttttaaagt tttcttatga ttaaaa
//
LOCUS       DAZULY010000431          636 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.447, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000431 DAZULY010000000
VERSION     DAZULY010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.447"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..92)
                     /locus_tag="ACG81A_002907"
     CDS             complement(<1..92)
                     /locus_tag="ACG81A_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HFS5016179.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLG"
     gene            complement(270..636)
                     /locus_tag="ACG81A_002908"
                     /pseudo
     CDS             complement(270..636)
                     /locus_tag="ACG81A_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          193 a          128 c           88 g          227 t
ORIGIN      
        1 cccaataaat aatttttata ttctgacgca tccattttac tacggagatt atctgccgca
       61 ctaaataact tctgatttaa ttctgcaccc aaaaagatac actcctatct cttatttaaa
      121 gttgctatat accctcttat tttagcatac tcagttttag aaaaagcaaa aaaataaaga
      181 aacttattct tgataacaag ctacgtgacg tatatcccta acacaaaaat agccatatcc
      241 tttggtttgg atacggctat ttgatattac tcattcaatt atcaaatcac agtaggtgtg
      301 aatctcatcg ggatcggcaa tatctacttc tgattaggat caaaggtgat tttctcaata
      361 aaggtgtgat acaaagcttt aacttgcttc ttctcactct ggataagaaa gcaatccaaa
      421 ctagtcaata tctgttggac atctttgact tgaatcggtt ccccttgttg ttgaaagatc
      481 cctaagattt cattctctcg aatttgtaac tgtcttcgtt tttcagtcaa ttcatttaaa
      541 cgatctttta acatcggaaa caattcggga ctatcttcca aagcagcttc ctgtttttgc
      601 tctaatagtt agattttgcg taccattttc ttcttt
//
LOCUS       DAZULY010000432          635 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.448, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000432 DAZULY010000000
VERSION     DAZULY010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.448"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..270)
                     /locus_tag="ACG81A_002909"
     CDS             complement(<1..270)
                     /locus_tag="ACG81A_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016180.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="ACG81A_002910"
     CDS             complement(276..>635)
                     /locus_tag="ACG81A_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFS5016181.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DAZULY010000433          620 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.449, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000433 DAZULY010000000
VERSION     DAZULY010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.449"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>620)
                     /locus_tag="ACG81A_002911"
     CDS             complement(<1..>620)
                     /locus_tag="ACG81A_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS5016182.1"
                     /translation="LSAAPYFRLLINESANLYAEFAHKSIELDNPSLLFIDKQQEVQI
                     FSTQKETQTFIIIQFYFDEQLLAHITGQSFSIDQEITSIITFLKNEQFKIDYLKHSKP
                     AISYDQEINSFFILCQQMSIHLLSLLVTYCLQKIQLSSLPRHYQQELALLTIPSNEKV
                     IPKKKHILYSDELVDPIIRYLEEHLSENFLLEQLAQIFFISPASLK"
BASE COUNT          220 a           70 c          113 g          217 t
ORIGIN      
        1 ttttaagctg gctggactga taaaaaaaat ttgggctaat tgttcaagca aaaaattttc
       61 tgaaagatgt tcttctaaat atcgtatgat tggatcaact aattcatctg aataaagaat
      121 atgctttttt ttaggaatga ctttctcatt tgaaggtatt gtcaataaag caagttcctg
      181 ttgataatgc cttggtaagg aagacaattg aattttttgc agacaataag taactaataa
      241 ggaaagcaaa tgaatgctca tctgttggca tagtatgaaa aaagaattga tttcctgatc
      301 ataggaaata gctggcttac tatgttttag ataatcaatt ttgaactgtt catttttcag
      361 aaaagtaatg atagatgtta tttcttgatc tatagaaaaa gattgtcccg taatatgtgc
      421 taataattgc tcatcaaaat aaaactggat aatgataaaa gtttgtgttt ccttttgagt
      481 agaaaagatt tggacttctt gctgtttatc aatgaataaa agggaaggat tatctagttc
      541 tatagattta tgtgcaaatt cagcatataa attagcgctt tcatttatta gtagtcgaaa
      601 atagggagca gcagataaat
//
LOCUS       DAZULY010000434          602 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.451, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000434 DAZULY010000000
VERSION     DAZULY010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.451"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..336)
                     /locus_tag="ACG81A_002912"
     CDS             complement(<1..336)
                     /locus_tag="ACG81A_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFS5016183.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISV"
BASE COUNT          182 a          107 c           84 g          229 t
ORIGIN      
        1 tactgagata tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt
       61 tatataacga gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc
      121 caattcactg acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt
      181 cagcttatcg atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt
      241 cgatattgac tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct
      301 gtgatcttct ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat
      361 attactttcc atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc
      421 actgttgtca gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt
      481 tggagagcgt tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt
      541 actagtatgc atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa
      601 cc
//
LOCUS       DAZULY010000435          573 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.452, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000435 DAZULY010000000
VERSION     DAZULY010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.452"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>573
                     /locus_tag="ACG81A_002913"
     CDS             <1..>573
                     /locus_tag="ACG81A_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS5016184.1"
                     /translation="EILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKER
                     DIYINECIEIVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKK
                     ITVNNYILDENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNN
                     KISNMDKIFDVKFDLNKENSNGLGLYIVSN"
BASE COUNT          256 a           40 c           84 g          193 t
ORIGIN      
        1 aagaaatttt aaaattagaa aagttaaaat atgacttttt taaaggtgca tcccatgaac
       61 ttaaaacccc tcttgctagt cttaaaataa tactggaaaa catgaaatac aatattggaa
      121 aatataaaga aagagatatt tatattaatg aatgtattga aatcgttgat agtttaacaa
      181 aaaatatttc tcaaatatta tcagttcatt ctatagaaaa tttgaataat gatgaagaat
      241 atttgaaaat aaatgataca ttagaagaca tattaaaaaa gtatgaaata ttagcaaatc
      301 aaaagaaaat aactgtcaat aattatatat tagacgagaa tgtttatata ggaaaaacag
      361 ctttaaagat tattctatct aatttgatta gtaatgcggt aaaatatact gatgtaaatg
      421 gggtaattaa tattgggata gttaatgatt ggttatatat agagaattca tacggtaaca
      481 ataaaatttc gaatatggat aaaatatttg atgttaaatt tgatttaaat aaggaaaata
      541 gtaatggatt aggtttatat attgtaagta ata
//
LOCUS       DAZULY010000436          559 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.454, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000436 DAZULY010000000
VERSION     DAZULY010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.454"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..532
                     /gene="spxA"
                     /locus_tag="ACG81A_002914"
     CDS             134..532
                     /gene="spxA"
                     /locus_tag="ACG81A_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFS5016185.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          197 a           88 c          110 g          164 t
ORIGIN      
        1 cattttttta aaaaaattat gaaaaaaagg tagctattta aaagttgatt atgtataata
       61 gaaacagatg ggagattatt atgatcttct gttacaaaaa agtgattctt atcacgaaaa
      121 ggagtggtgc acgttgttaa cattatatac ttccccaagt tgtacttcct gcagaaaggc
      181 tcgtgcgtgg ttgcaagaac atcagattcc atttgttgaa cgtaatattt tttcagaacc
      241 attgaacagt tctgaattaa aagcgatttt acaaatgaca gaagacggaa cagaagaaat
      301 catctcgaca cgttccaaag tattccaaaa actgaacatg gatttagatg atctgccgtt
      361 acaagaatta ttggaacttg tgcaaaacaa tcctggattg cttcgccgtc caatcatgat
      421 tgatgataaa cgtctgcaag ttggttttaa tgaagatgaa attcgtcggt tcctaccgcg
      481 cgatgtacga caattagaat tacgtcaagc acaattgatg gctggattat agaaggataa
      541 tacgaaactt ggaaaagac
//
LOCUS       DAZULY010000437          558 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.455, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000437 DAZULY010000000
VERSION     DAZULY010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.455"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            176..>558
                     /locus_tag="ACG81A_002915"
     CDS             176..>558
                     /locus_tag="ACG81A_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFS5016186.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT          211 a           91 c           82 g          174 t
ORIGIN      
        1 ataaagataa ctatccatta tcaactaaaa aaacattttt gaaaataaac atcagcagtc
       61 ctatcaatgc atgtgagctt atagaagtta ttcgcagcgc agcataattg cattagcttc
      121 ttttcgtgtt ttttgcctct tttcccttta aatcttaatg gaggaggtgt gcaatatgtc
      181 agataacgac gaacgcgtaa atgcccagtt gattcgattc tttgaaaagg tcattttgaa
      241 caccgctaat acttattata aaaaacaaga agaaattcaa aaacatgaac aacttgatca
      301 actcccacca tatttgttta ctaaagatac tataaaattt aaacaaccga ttaatgtttt
      361 aaatgtgact ctccttgttg aagatataaa attagcaaaa atccttcctt tgttgaaaga
      421 acctgaacaa atttttctta tcgaaaaatt tatctttgat aaaactgata aagaaattgg
      481 aaaggttctg gggattacaa gacagggtgc gacaaattta aaacatcgac tttacaaaaa
      541 aattcaaaaa atgctggt
//
LOCUS       DAZULY010000438          548 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.456, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000438 DAZULY010000000
VERSION     DAZULY010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.456"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..450)
                     /locus_tag="ACG81A_002916"
     CDS             complement(73..450)
                     /locus_tag="ACG81A_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016187.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
BASE COUNT          169 a           96 c           99 g          184 t
ORIGIN      
        1 caataatagg aacctgattt taaaagcggc tacctcttta ccgtgcctct ttacatacaa
       61 aaaatagagg aattaagcgt aaatggcttg gctattataa ctaaaatgct ggttatggag
      121 atgttgcagc aagttttcga gaaaagcgta ttccatctct tgtccattga ataagatctg
      181 gatacgataa tgatttttga aacgcttctg ttcatgaatc tgactgattg aaagttcgcc
      241 tttgtgttct gtctgataaa gaaaagtcct tttacggcat tcagcacagc gcttggttgc
      301 tcggcggatt tttcggaatg tataaaaact agtatcttta ttgcataaat aaacgtcttt
      361 gatcgacagc aaatcattat atagacagga acgttcttca gctgttaatt ctgataaaaa
      421 aagctgacct tgttttattg catcttccat cggcaagtgc ctcctcgaac ccattatcca
      481 aatttttagt taattctaat ataccatcat taagtataaa tgtaaacgct tgttgatggt
      541 ataagatt
//
LOCUS       DAZULY010000439          526 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.457, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000439 DAZULY010000000
VERSION     DAZULY010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.457"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ACG81A_002917"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ACG81A_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFS5016188.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DAZULY010000440          507 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.460, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000440 DAZULY010000000
VERSION     DAZULY010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.460"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..506
                     /locus_tag="ACG81A_002918"
     CDS             <1..506
                     /locus_tag="ACG81A_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HFS5016189.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIK
                     VLMGITA"
BASE COUNT          176 a           96 c          103 g          132 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag accatcgacc tattaaacga cgaaataaat
      361 tttatcaaag tctccgtaca gcctcttcca cgattaaggg catggagacc attcgaggaa
      421 tatataaaaa gaaccgaaga aatggaacgc tcttcggctt ttcggtgtct actgaaatca
      481 aggtattaat gggaataaca gcctaag
//
LOCUS       DAZULY010000441          494 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.461, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000441 DAZULY010000000
VERSION     DAZULY010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.461"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..433
                     /locus_tag="ACG81A_002919"
     CDS             <1..433
                     /locus_tag="ACG81A_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016190.1"
                     /translation="FFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTI
                     YKDNGFIEDKNSSIPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLC
                     NIEGNPSCKKNAYDIERLLKQKTMKNILKNVKIAESLDDIL"
BASE COUNT          196 a           55 c           93 g          150 t
ORIGIN      
        1 attttttacg tctgtttatg attttgaggg cggagaacct gtcggagaat atgaattagt
       61 atgcaaaata gcagacttag taggaaaaga taatctaatt gttaagacgc atcctagaga
      121 tgtaagaaca atttataaag ataatggatt tatcgaggat aaaaattcat caataccttg
      181 ggaggcgatt caattgtcag gcgattttaa cgataaaata tttatgacaa ttaattcagg
      241 aagtgttatt tcaggtagta caatgtctgc aaaacctgta cgcacgtatt atatgtataa
      301 attgtgtaac atagaaggaa atccgtcttg caaaaagaat gcgtatgata ttgagcgatt
      361 gcttaagcaa aaaacgatga aaaacatttt aaagaatgtg aaaatagcag aaagcttaga
      421 tgatatacta tgagcaaaac taaatattta attaaaaata tgggaatatt aacaataagt
      481 aatttttctt caaa
//
LOCUS       DAZULY010000442          485 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.462, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000442 DAZULY010000000
VERSION     DAZULY010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.462"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..259)
                     /locus_tag="ACG81A_002920"
     CDS             complement(62..259)
                     /locus_tag="ACG81A_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5016191.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DAZULY010000443          475 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.463, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000443 DAZULY010000000
VERSION     DAZULY010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.463"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>475
                     /locus_tag="ACG81A_002921"
     CDS             <1..>475
                     /locus_tag="ACG81A_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFS5016192.1"
                     /translation="TAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPE
                     EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPK
                     DTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          192 a           72 c          112 g           99 t
ORIGIN      
        1 tcaccgcagg agaaggaaca agtattgaaa gtggaacaac agtctttgca gtgaaagacg
       61 gcgtaagctt accagaagat aaacttccag tgttgaaagc aaaagatggt tatacagatg
      121 cgaaatggcc agaggaagca actcaaccaa ttacagcaga tgatacagaa tttgtatcaa
      181 gtgcaacaaa actggatgat aaatctgacg ctgacaaata taatcctgaa ggtcaaaaag
      241 tgactacaga attgaataag gaaccagacg catttgaggg aattaagaac aaagaagatt
      301 taccaaaaga tactaagtat acttggaaag aaaaagtaga tgttagcgca gctggaaata
      361 aaaaagggac tgttgtagtg acatatccag atggatcatc tgatgaagtt gaagtagatg
      421 ttacagtgac agacaatcgt tctgacgccg ataaatatga gccaacagta gaagg
//
LOCUS       DAZULY010000444          473 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.464, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000444 DAZULY010000000
VERSION     DAZULY010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.464"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..246
                     /locus_tag="ACG81A_002922"
     CDS             52..246
                     /locus_tag="ACG81A_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016193.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          203 a           61 c           83 g          126 t
ORIGIN      
        1 cttacatata tttgaatatt aactaaaaac agcaagaaag aagaggatca aatgtattat
       61 gtagaagtaa aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat cagtaacgaa
      121 tatcctttac ttggttcatg gaaagaagct gctccttttt ccaaaccatg tgccataaaa
      181 ataaagaacg agctagaaaa agaactgact tgtggtaaag ctgttgtaga tatcatcgaa
      241 aagtaaatcc aatgtataga aaggaagatc acacatgaaa agcgacgtac aaattgctaa
      301 gattatagaa ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt
      361 ataccatatg attatttcaa tatgtatcct ggaattggag atctcgtaga gctatatcaa
      421 gatgaatcaa atatacttgt cattcatcat gaaatataga gacttataaa att
//
LOCUS       DAZULY010000445          471 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.465, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000445 DAZULY010000000
VERSION     DAZULY010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.465"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          215 a           63 c           37 g          156 t
ORIGIN      
        1 aatttagttt taaaattaaa gataattaag tactaccagt gtcaaaacaa aaaataaatt
       61 aagaactacc ttatgcatat aaaacatact accattacta gtaatatata ctaccttatt
      121 tcagtagtat atattatatt attcttataa gacaaggctt taggctagaa aacaatagat
      181 tatatactac taaaaaatac aataagtact accaatgtca gatatatata ataattatgt
      241 actaccatta aaattaactt atttaataag atactactgt gttaatctga aaaaaattac
      301 tttaaagtag ttacatacta cctgttaatt tagatactac cattaatact taaattagat
      361 actacctata tttatattaa gtactaccgt tctaagaata agataataaa aaaatactac
      421 caaattgcaa ttaaatacta cttaaaatgt aaaaaataag aactacccaa a
//
LOCUS       DAZULY010000446          461 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.466, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000446 DAZULY010000000
VERSION     DAZULY010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.466"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          165 a           86 c           65 g          145 t
ORIGIN      
        1 tcaataatta tcaaactgag tcatatagag acaaaaattt caaaaatgac ctgttaaatg
       61 gtcatcgaat atcatttaaa atcaatacga cggagtcaga cacggagtca aaacaagccc
      121 gctgagagta aaaaatatac agtcccatag gtaaagtgcc acaggactgt atattttttt
      181 atttgagatt acctgaaatg attccattta atttttatct cattttttcc tactagttag
      241 tatcaaatac tctcctattt tctcaataaa ctattttggg ttacatggta tctgaaacag
      301 ctaaaacctt gtcattatag caaaaaatca ctttatctcc gtttcatttc agatacattt
      361 cacttaagcg gattccaaaa aaacaaaaga aaaaggaacc cctagttagc ctatgctttt
      421 tattggttcc tctcaaaaca cggtcatata acaacggggt g
//
LOCUS       DAZULY010000447          452 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.467, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000447 DAZULY010000000
VERSION     DAZULY010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.467"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>452
                     /locus_tag="ACG81A_002923"
     CDS             <1..>452
                     /locus_tag="ACG81A_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016194.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGST"
BASE COUNT          165 a           68 c           92 g          127 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca ac
//
LOCUS       DAZULY010000448          446 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.469, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000448 DAZULY010000000
VERSION     DAZULY010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.469"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            92..391
                     /locus_tag="ACG81A_002924"
     CDS             92..391
                     /locus_tag="ACG81A_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFS5016195.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          172 a           54 c           90 g          130 t
ORIGIN      
        1 atccagtaat gatttaaaaa aatctagatg tagcgtatac tttgtttata gaatgtagtg
       61 ggaagaaata tatttttggg aggaaatcgt gatgtctaaa agtgaagaaa tagaaaacat
      121 ggtactaaaa ttatatgatc tcgttttaaa tccagagatc aaagaaaagg aacgtatact
      181 tttgatagac gcaaagacag gcttagaaaa aggtcagtat tatcctaaag tcataaataa
      241 tttggaacgt agtttacgtc cgttagcaat caggggagaa ttgtctaaac cagtatcacc
      301 gttttatatg gagatatcaa caataggaaa atttgaaaaa gaattaggtc gaggaatggc
      361 ttcggcaccg attacttttg ggcatttata aaaacgagtt attcatctca atgaattaaa
      421 aagtatagtc aatctcataa tgcagg
//
LOCUS       DAZULY010000449          439 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.470, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000449 DAZULY010000000
VERSION     DAZULY010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.470"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>439)
                     /locus_tag="ACG81A_002925"
     CDS             complement(<1..>439)
                     /locus_tag="ACG81A_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5016196.1"
                     /translation="SFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRS
                     HASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQ
                     LTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQK"
BASE COUNT          130 a           86 c           83 g          140 t
ORIGIN      
        1 cttttctgtc ctactttacg aagttcattc atttcacgga tacgcaactc attaaaggct
       61 tgattcatgt gtttgataat atgaaaccga tcaatcacta ctttcgcatt tggcagaata
      121 cgtttggtaa gctggaagta ggcggcgttc atgtctgtga ctaaaaattt tacttcttca
      181 ggattagcgc agcctaagaa atagcttgtt aatcgaggca atttacgcgt aggcaagaca
      241 tcaattaatt gtcctgtttc gccatctgcg caaataaagc tcattttatc ttctatggaa
      301 gcgtgcgaac gaaattcatc aaccatcaat actctgggga gaatcctctt agattgcttt
      361 ggtaagtagc ttttaaactc tttcaacgta cgaataacgg tggtcaaaga tacctgacaa
      421 tttttcgcaa taaaagata
//
LOCUS       DAZULY010000450          425 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.471, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000450 DAZULY010000000
VERSION     DAZULY010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.471"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..363)
                     /locus_tag="ACG81A_002926"
     CDS             complement(<1..363)
                     /locus_tag="ACG81A_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016197.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQ"
BASE COUNT          139 a           81 c           71 g          134 t
ORIGIN      
        1 ctgtagttta taaaaaaacg gcgaatgaag atgtgttttc atatgaccaa aatataataa
       61 ttctgaaatt tctaaaggtg tcaattcttt cagcatcgtt aaatcagtaa agtcgatcca
      121 cttcaattca tcgaatccac gcctgttacg agaagaggaa aagaaacgct gaatattttc
      181 tgtaccacgg attactttca gccctgtatg ggattcatat tctccagcag aagcagatgg
      241 atcaagcaag agcaagtttt gaggaaaatg cacgatactg tttgcaaagt cagtagcagt
      301 cagccctttt gttaaaacag cattacttgt ggtatcaata tgtacataaa ctgactcagt
      361 catcatgttc ctcctcttat ttcctcttaa tctcttttat tttacaacat ttgcattaaa
      421 aaagc
//
LOCUS       DAZULY010000451          414 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.473, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000451 DAZULY010000000
VERSION     DAZULY010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.473"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>414)
                     /locus_tag="ACG81A_002927"
     CDS             complement(<1..>414)
                     /locus_tag="ACG81A_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016198.1"
                     /translation="TFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQKWY
                     WLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLSAG
                     FLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHIL"
BASE COUNT          156 a           77 c           73 g          108 t
ORIGIN      
        1 tttaaaatat gtgtcagcat atcactagga gccataccac ttaattcttt tcccagtggg
       61 actttcaatt cttgtgctct agattgttga ataaaaggaa ttaagaatcc cgcacttaaa
      121 aaaaaggtga ggacggtagc ttttaaaaga cttaagagtc tagcgatttt ttggtcccaa
      181 aaataaaaag aaagaaacaa cgttcctcca ataaatacag aaaccatagc gacagaaagt
      241 aaatgagtat acactactag cgtcatgcca atagacaacc agtaccattt ttggtaatct
      301 ctaatataaa tttcgtaaca ccccattagt atcaaaggca aaaatgttaa agcgacagct
      361 tctcccaatg aagcacgatg gaatatgtca atggcacgat aggcggaaaa tgta
//
LOCUS       DAZULY010000452          413 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.474, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000452 DAZULY010000000
VERSION     DAZULY010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.474"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          138 a           67 c           89 g          119 t
ORIGIN      
        1 ggaagaggtt tggacaaaaa tggaaatttc cgccaaaagt aactgctcct caactgaaat
       61 caattaaaga actagaattt gagaatgcac tggtaatttg tatgacatgt actgatgaag
      121 tatttcgaaa acatttttaa aggttgagat gtatatagat ttaaccaatc atttatctct
      181 ctcggaaaag gaatattaac taaccagcgg gttatcaaaa ataggtagga aaactgtact
      241 taatggctta tttcgcgctg attatgagaa tatcagtctg tttgattaat ttttagggtt
      301 caaaatacag agacaaactc caggtcgggt ttcgccacaa gcgaattacc cgatctggag
      361 ttttgtctat tgcaggaagg cgtagccgga ctgcaggaat aaagcccccg gtt
//
LOCUS       DAZULY010000453          403 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.475, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000453 DAZULY010000000
VERSION     DAZULY010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.475"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          156 a           47 c           84 g          116 t
ORIGIN      
        1 cattggtcgt tgaatgggct gaatagtagt gtataaaaca attttgtgga gcgtgaagct
       61 tttatggtag aggaaaatac gaataaagtg atgttgataa aaagattcct atcttgaata
      121 tgaatcgaga tgtcttgaaa atgaatgagt ttgaccaatc attatcctat cgaaatttac
      181 agtttcgttc gattggtctg tcaattaatg aaaaagtaaa aaagaatatg ttctaggaag
      241 agggcggcac atctaaattt ttaagtaacg gacagttcaa atatcaagca ttgaaagagg
      301 ctatcctact gtttgaagga atacgaatat ataaaaacga caaagaggaa aaagtagttc
      361 attaaatccc ttcaagtgat gaaagaatga gatctaataa aac
//
LOCUS       DAZULY010000454          402 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.476, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000454 DAZULY010000000
VERSION     DAZULY010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.476"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..112
                     /locus_tag="ACG81A_002928"
     CDS             <1..112
                     /locus_tag="ACG81A_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS5016199.1"
                     /translation="MYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            113..>402
                     /locus_tag="ACG81A_002929"
     CDS             113..>402
                     /locus_tag="ACG81A_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFS5016200.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFL"
BASE COUNT          152 a           49 c           83 g          118 t
ORIGIN      
        1 tatgtatgag aaggagttga aatatttctt tgaaaacgta gtaacaggca aagaaaactg
       61 gaatgattta tatcatgcaa acgaagtatt gaaaatagct gaggagaaat aaatggaaat
      121 tttatttact atttgtggta gagctgggtc taaggggttt aaaaataaaa atttgaaata
      181 tttggcagga aagcccctag tccatttcgc tttagcaatg atagatatct atcaacaaaa
      241 acatcctgaa cataattgca cgacttgttt gaatacggac agtaatgagt tagtagaatt
      301 gacgactaag ttaaatccgt ctgtgaaaat tgtagaacgt aaacctgaat tagctacaga
      361 tactgtaggg aaaattgaag tgatacgtga ttcattctta ca
//
LOCUS       DAZULY010000455          401 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.477, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000455 DAZULY010000000
VERSION     DAZULY010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.477"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          112 a           98 c           76 g          115 t
ORIGIN      
        1 attataaata actcataaaa aatattttga ctttcaaaat cacttttgct accagaagtt
       61 gaggcgaagt gttttaagca tagttgtacc ctagtttgac cgttaaaaat aaataacgat
      121 cgctagagtg caactatgtt tgtttgccct accgtcagcg tcggcttggc ctctcacatc
      181 cattaccgtc gggattatct ccgttacata cccttacaaa atacgccctg taagcattgc
      241 atgaaaccac cgtgcatttg caccgtattt ccgccctagc cgttcaaatc ggtcgctacc
      301 accattcaga aacacgtgcg gtaatggaag gtttggagga agctatccag accactaacg
      361 attgtcccta tcgtcgtacg tttcactcag atcaaggttg g
//
LOCUS       DAZULY010000456          377 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.478, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000456 DAZULY010000000
VERSION     DAZULY010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.478"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>377
                     /locus_tag="ACG81A_002930"
     CDS             <1..>377
                     /locus_tag="ACG81A_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1W family transposase"
                     /protein_id="HFS5016201.1"
                     /translation="KLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLT
                     LDIWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHR
                     TIKYLNNLIEQDHRPVKRRNKFYR"
BASE COUNT          144 a           64 c           69 g          100 t
ORIGIN      
        1 aaactactct atcaaatttg gaaaaagaaa aataaaaaat ccttttattc atggaaaatg
       61 gatgaaacgt acatcaaaat taaaggaaaa tggcattatt tgtatcgagc catcgatgca
      121 gatggtttaa ccttggatat ttggttacgt aaaaaacggg acacacaagc agcctatgct
      181 tttcttaagc ggttagtgaa gcagtttgat gaaccgaagg ttgtagtcac agataaagcc
      241 ccctctatta caagtgcctt taagaaacta aaagaatacg gcttttatca agggacagaa
      301 catcgtacca ttaaatacct gaataatttg attgaacaag accatcgtcc agtaaagaga
      361 cgcaataaat tctatcg
//
LOCUS       DAZULY010000457          375 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.480, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000457 DAZULY010000000
VERSION     DAZULY010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.480"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            75..>375
                     /locus_tag="ACG81A_002931"
     CDS             75..>375
                     /locus_tag="ACG81A_002931"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFS5016202.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKLPT"
BASE COUNT          143 a           45 c           70 g          117 t
ORIGIN      
        1 tataattagt atatgttttg tgagaaattt agacttacaa taagcagtga atttataatc
       61 aacgtatagg agacatggat gaaatgaaac taattggtat agatttagac ggtactcttt
      121 tgaattctga ccagagcatt agtcaaaaaa atgcaagaac aatgaaacat ttaccggaag
      181 actgttttcc attcatttgt tctggaagag aagtagaaga cattaataat attctcaaaa
      241 aatctggttt aaggatacca gcagtagggt taaatggttc tgttggattt gatggagaaa
      301 aaaagatatt tgagtcttcc ttcgataaaa atgatgtaaa aaaaattgat tctgtcctat
      361 cgaaattacc aacca
//
LOCUS       DAZULY010000458          360 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.481, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000458 DAZULY010000000
VERSION     DAZULY010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.481"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..252)
                     /locus_tag="ACG81A_002932"
     CDS             complement(34..252)
                     /locus_tag="ACG81A_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016203.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT          128 a           63 c           61 g          108 t
ORIGIN      
        1 cttgtctttt gaataattag cagtcaatcc ttattatgat tgcctggatc tgtagtaggg
       61 ctcatgatag aaatgatctt catgaattct gcgccgctca gattgacggt acttcttttc
      121 atcccacaac atgaaccaca aagtaaataa cggaacaaac acaagtaaag ctggacgtat
      181 gccgaaatta ccaataataa tacctaaaaa caaaacagtt aatactaagc caagacgtct
      241 cattgatttc atgaattctc acctctttaa tttatgcgaa caattgttcg tatatttagt
      301 atacgaatat ttgttcggtt tgtaaagagg gaagtgataa aaatttgaaa gaagaagaac
//
LOCUS       DAZULY010000459          355 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.482, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000459 DAZULY010000000
VERSION     DAZULY010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.482"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..>355)
                     /locus_tag="ACG81A_002933"
     CDS             complement(66..>355)
                     /locus_tag="ACG81A_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016204.1"
                     /translation="LMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLT
                     AGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT          117 a           67 c           60 g          111 t
ORIGIN      
        1 ttcttacgaa aaaatgacaa agaaaaaagt ttgtgacatg acttttgtca caaactctcg
       61 tattcttaac caaaatattg atgaaatact tgtgtaataa ctggtagaaa ataaataatg
      121 atcatagccg ctaccaccac caacaattgt tttcgctgtt tctttcgctt tagcatgctt
      181 gtcgttttat gaaaaaaata agtagcgctt ttagctcctg cagtcaaagt cgataaaatc
      241 aacgttggtg aatatgctgt ttgattgtgc gtcaatgaca acaacttcgt cccgtaatca
      301 ggatacttta gaaatgcttg gtacacagcc acaatcgtga ttgctcccat aagat
//
LOCUS       DAZULY010000460          343 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.483, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000460 DAZULY010000000
VERSION     DAZULY010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.483"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..321)
                     /locus_tag="ACG81A_002934"
     CDS             complement(<1..321)
                     /locus_tag="ACG81A_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFS5016205.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT           96 a           55 c           63 g          129 t
ORIGIN      
        1 taaatggata atagtggttt ccaatgtatc gtttgttctt ttgtaggctg gtaaagtttg
       61 ttgtttaaac tcaccattac gatctctagg tatttccaat gttaattcac catattcggt
      121 tttgattgat cgaaagtaag aaccgtttct cgaattacct gaattaaaac cagtgcgatc
      181 atatttttcg taatctaaaa aagccgttaa ttcagtccgt aggagtgtgt ttatcgcttt
      241 ttctaagtgc gaacggaata attcatttaa atcgccttta gtgactagag tttgcacaat
      301 ttctgtagta aaatcattca tagggaagtc ctcttttctg tga
//
LOCUS       DAZULY010000461          331 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.484, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000461 DAZULY010000000
VERSION     DAZULY010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.484"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..>331
                     /locus_tag="ACG81A_002935"
     CDS             51..>331
                     /locus_tag="ACG81A_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS5016206.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEA"
BASE COUNT          115 a           54 c           71 g           91 t
ORIGIN      
        1 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
       61 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
      121 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
      181 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
      241 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
      301 aagggttagg aaaaatattg attgaagaag c
//
LOCUS       DAZULY010000462          309 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.491, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000462 DAZULY010000000
VERSION     DAZULY010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.491"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            37..240
                     /locus_tag="ACG81A_002936"
     CDS             37..240
                     /locus_tag="ACG81A_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HFS5016207.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
BASE COUNT          121 a           33 c           68 g           87 t
ORIGIN      
        1 aaaaaatgat tatgaaaaag aaagaaaaag aaagtattga aacattgtgt tattctagga
       61 atattaggaa cttgtctagc tggcattggt acaggaatag acgttgacgc agctacttac
      121 tatggaaatg gtctttattg taacaaagaa aaatgttggg taaattgggg acaatcatgg
      181 tcagaagggt taaaacgttg gggagataat ctttttggta gttttagtgg aggacgataa
      241 tgacttcaat aaacattgat cacacaaaaa aagtcataca tgaattatac aatagtttaa
      301 tgacacgac
//
LOCUS       DAZULY010000463          309 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.492, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000463 DAZULY010000000
VERSION     DAZULY010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.492"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..309
                     /locus_tag="ACG81A_002937"
                     /pseudo
     CDS             1..309
                     /locus_tag="ACG81A_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DAZULY010000464          308 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.493, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000464 DAZULY010000000
VERSION     DAZULY010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.493"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..>308)
                     /locus_tag="ACG81A_002938"
     CDS             complement(76..>308)
                     /locus_tag="ACG81A_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5016208.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           94 a           46 c           56 g          112 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaata
//
LOCUS       DAZULY010000465          283 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.497, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000465 DAZULY010000000
VERSION     DAZULY010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.497"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACG81A_002939"
     CDS             <1..156
                     /locus_tag="ACG81A_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFS5016209.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT          112 a           23 c           57 g           91 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taa
//
LOCUS       DAZULY010000466          283 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.498, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000466 DAZULY010000000
VERSION     DAZULY010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.498"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..280
                     /locus_tag="ACG81A_002940"
     CDS             <1..280
                     /locus_tag="ACG81A_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016210.1"
                     /translation="SRAYYSAFKKSDEYLKENYDIIYNGSSGKGSHQTVWNLFSTVKE
                     LNTLGIHNSGYRMLEKRKCADYIPNETITKTDMALMNREAEKIINKLT"
BASE COUNT          119 a           35 c           54 g           75 t
ORIGIN      
        1 ttcaagagca tactacagtg cttttaaaaa atctgatgag tatttgaaag aaaattatga
       61 tattatttat aatggaagta gcggaaaagg aagccaccaa accgtttgga atttgttttc
      121 aacagttaag gaattgaata ccttaggaat acataatagt gggtatagaa tgttagagaa
      181 aagaaaatgt gctgactata ttcctaatga aaccattacg aaaacagata tggcgctgat
      241 gaatcgagaa gcagaaaaaa taataaacaa actcacctaa tga
//
LOCUS       DAZULY010000467          279 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.499, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000467 DAZULY010000000
VERSION     DAZULY010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.499"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..>279
                     /locus_tag="ACG81A_002941"
     CDS             33..>279
                     /locus_tag="ACG81A_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFS5016211.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLW"
BASE COUNT          102 a           32 c           53 g           92 t
ORIGIN      
        1 ctctattagg aaatataaga aggagtataa aaatgaacaa acataataga aaaataacga
       61 ataacaaaga actgatcttg ttactaatag ggatttgggg tatcttttta ctctttaata
      121 tgtatatgcc gactatgaga gcggatgacg tagtttatgc aagtagatta gatgaacttg
      181 gctatttggg tgcttcgata gaacattata agacatggag ttcaagaatt attatagaac
      241 tattcttaat gttcttttct aaacacttta tgctatgga
//
LOCUS       DAZULY010000468          265 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.502, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000468 DAZULY010000000
VERSION     DAZULY010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.502"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>265)
                     /locus_tag="ACG81A_002942"
     rRNA            complement(<1..>265)
                     /locus_tag="ACG81A_002942"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           56 a           89 c           51 g           69 t
ORIGIN      
        1 actcctcttc agacttactg cttggacaga catttccgat cgtctgcatt ccttagcctc
       61 ctgcgtccct ccattgctca aacaaaaaca acgggtacag gaatatcaac ctgttatcca
      121 tcgcctacgc ctgtcggcct cggcttaggt cccgactaac cctgggcgga cgagccttcc
      181 ccaggaaacc ttagtcattc ggtggacagg attctcacct gtctttcgct actcataccg
      241 gcattctcac ttctaagcgc tccag
//
LOCUS       DAZULY010000469          263 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.504, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000469 DAZULY010000000
VERSION     DAZULY010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.504"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>263
                     /locus_tag="ACG81A_002943"
     CDS             <1..>263
                     /locus_tag="ACG81A_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFS5016212.1"
                     /translation="TTTEFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADA
                     NANVVIITTRQKLDAALKYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT           96 a           49 c           57 g           61 t
ORIGIN      
        1 accaccacag agtttacaaa atttgcttcg gaatttaata ctgggatttc ttccgggaac
       61 gccaaagcga atagcttgat cgttggaacg ggaagtgcca aagaattaag tgaaaccctg
      121 ttggcagatg ccaatgcaaa tgttgtaatc attacaaccc gtcaaaaact agatgcggcg
      181 ttgaaatatg caaagaaaca agaagagaaa aaaggaacca atcgcttcca aaaattaatg
      241 ggccaacata tcgtgtttgt agt
//
LOCUS       DAZULY010000470          255 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.505, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000470 DAZULY010000000
VERSION     DAZULY010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.505"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           79 a           46 c           39 g           91 t
ORIGIN      
        1 aattggagtg gtagttaatt agtgacttat ttttgatttt atagcactga tactataaaa
       61 tatagatatc atcatattac acaatcttaa tactaactta aaaaatatct cctttcacaa
      121 gtatggtgat aaaattcgtt ccgggctacc tttttaggta gcctacttta atctttatac
      181 ctttctggat caacgaaagt atactttata tagtcataac gccgatgatc gctacgtgcg
      241 tccggcacgt cagtc
//
LOCUS       DAZULY010000471          252 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.507, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000471 DAZULY010000000
VERSION     DAZULY010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.507"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..>252)
                     /locus_tag="ACG81A_002944"
     CDS             complement(32..>252)
                     /locus_tag="ACG81A_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFS5016213.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT           73 a           61 c           41 g           77 t
ORIGIN      
        1 aggctcatcg cagtaatagt aattttcttt ttcatttgtt tcctccttct tcgctttcag
       61 ccgagaacat tttgtaggtt cgatttgata cacccaacac actccctaaa aacgcgccaa
      121 aaccagtaat gatgacaaca cagatatctg tgtactgcca attgagcgct ttaccaacta
      181 accccacgaa agtagctagt gcgggaataa ttaccagtgc gaaccatttt agtacttcga
      241 acgttttatt at
//
LOCUS       DAZULY010000472          239 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.512, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000472 DAZULY010000000
VERSION     DAZULY010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.512"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>239
                     /locus_tag="ACG81A_002945"
     CDS             <1..>239
                     /locus_tag="ACG81A_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFS5016214.1"
                     /translation="AIIALKPEGRKAVDIENSINEWLQSVPKNLFKSITFDCGKEFSN
                     WKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT           84 a           46 c           43 g           66 t
ORIGIN      
        1 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
       61 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      121 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      181 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DAZULY010000473          234 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.516, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000473 DAZULY010000000
VERSION     DAZULY010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.516"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            11..208
                     /locus_tag="ACG81A_002946"
     CDS             11..208
                     /locus_tag="ACG81A_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFS5016215.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
BASE COUNT           85 a           27 c           53 g           69 t
ORIGIN      
        1 tgattagatt atgaaacatt taaaaatttt gtctattaaa gagacacaac ttatctatgg
       61 gggtaccact catagtggaa aatattatgg aaatggagtg tattgcacta aaaataaatg
      121 tacggtcgat tgggccaagg caactacttg tattgcagga atgtctatag gtggtttttt
      181 aggtggagca attccaggga agtgctaaaa tgaaaaaaaa tgctaagcaa attg
//
LOCUS       DAZULY010000474          229 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.517, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000474 DAZULY010000000
VERSION     DAZULY010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.517"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           73 a           31 c           42 g           83 t
ORIGIN      
        1 gtattaaata tactgcaagg caagacattt ggtaattgaa gaataacaag caaaaaaata
       61 gcagtcaggt ctcttgacaa agtatagttg ctattctttt tttaggccct actttaatga
      121 aatttttgtt tggagggaac gacttcattc ctttgattat ttcagttgaa tttaatagag
      181 aagcctggcg tctattaaga aatacttttc ttgatgagcg tcaattttt
//
LOCUS       DAZULY010000475          228 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.518, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000475 DAZULY010000000
VERSION     DAZULY010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.518"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>228)
                     /locus_tag="ACG81A_002947"
     CDS             complement(<1..>228)
                     /locus_tag="ACG81A_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016216.1"
                     /translation="SLGVYILHENHYFIDGMKSRGLIWDGIFGLGKRFLDRDFWIWFI
                     IAVVVVCIICMAIDVVREKFERQIFKIKSVK"
BASE COUNT           85 a           56 c           13 g           74 t
ORIGIN      
        1 ttctttacac tcttaatttt aaaaatctgt ctttcaaatt tttctcttac aacatcgata
       61 gccatgcaaa ttatgcacac aactacaact gcaattataa accaaatcca aaaatcccta
      121 tctaaaaacc ttttgcctaa tccaaatatc ccatcccata ttaagcctct agacttcata
      181 ccatcaatga aataatgatt ttcatgtaaa atatataccc ctaaactg
//
LOCUS       DAZULY010000476          225 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.519, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000476 DAZULY010000000
VERSION     DAZULY010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.519"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           91 a           36 c           29 g           69 t
ORIGIN      
        1 attgtgatcc acctcttttg tttattagtt taaaccatca aaccattttt aataaaatat
       61 gtaaattcct ttgctcaaaa aagtagcttc tttacgattc gacaatttca aataaagcag
      121 gtaaaaactg gctgtcagaa aatagccaaa ctgaagcaaa aaataatgtt aaaattaatt
      181 aatgaacgaa aaaaatccct cacctaatat tacggtgagg aattg
//
LOCUS       DAZULY010000477          224 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.521, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000477 DAZULY010000000
VERSION     DAZULY010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.521"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>224
                     /locus_tag="ACG81A_002948"
     CDS             <1..>224
                     /locus_tag="ACG81A_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFS5016217.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAZULY010000478          220 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.denovo.522, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000478 DAZULY010000000
VERSION     DAZULY010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.denovo.522"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>220)
                     /locus_tag="ACG81A_002949"
     CDS             complement(<1..>220)
                     /locus_tag="ACG81A_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS5016218.1"
                     /translation="NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCF
                     EELKSAIDSYIYYYNNERIKQKLNWQSPV"
BASE COUNT           63 a           40 c           32 g           85 t
ORIGIN      
        1 acgggacttt gccagtttaa tttctgtttt atccgttcat tattgtagta atagatatag
       61 ctatcgatgg ctgattttaa ttcctcgaaa cagttataaa ctttcccgtg aaaaatctct
      121 tgttttaata agccaaaaaa attctccata agtgaattat ctaagcagtt ccctttccga
      181 gacatgcttt gaaagatttt gtactccttc aatttgtttc
//
LOCUS       DAZULY010000479        13979 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.guided.054, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000479 DAZULY010000000
VERSION     DAZULY010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.guided.054"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1224)
                     /gene="hflX"
                     /locus_tag="ACG81A_002950"
     CDS             complement(<1..1224)
                     /gene="hflX"
                     /locus_tag="ACG81A_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFS5016219.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKN"
     gene            complement(1226..2143)
                     /gene="miaA"
                     /locus_tag="ACG81A_002951"
     CDS             complement(1226..2143)
                     /gene="miaA"
                     /locus_tag="ACG81A_002951"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFS5016220.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2147..2884)
                     /locus_tag="ACG81A_002952"
     CDS             complement(2147..2884)
                     /locus_tag="ACG81A_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS5016221.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2975..3502)
                     /locus_tag="ACG81A_002953"
     CDS             complement(2975..3502)
                     /locus_tag="ACG81A_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016222.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3682..4275
                     /gene="clpP"
                     /locus_tag="ACG81A_002954"
     CDS             3682..4275
                     /gene="clpP"
                     /locus_tag="ACG81A_002954"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFS5016223.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4379..4864)
                     /locus_tag="ACG81A_002955"
     CDS             complement(4379..4864)
                     /locus_tag="ACG81A_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFS5016224.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5017..5214)
                     /locus_tag="ACG81A_002956"
     CDS             complement(5017..5214)
                     /locus_tag="ACG81A_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016225.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5309..7069)
                     /gene="efrB"
                     /locus_tag="ACG81A_002957"
     CDS             complement(5309..7069)
                     /gene="efrB"
                     /locus_tag="ACG81A_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFS5016226.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7066..8796)
                     /gene="efrA"
                     /locus_tag="ACG81A_002958"
     CDS             complement(7066..8796)
                     /gene="efrA"
                     /locus_tag="ACG81A_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFS5016227.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9189..9401
                     /locus_tag="ACG81A_002959"
     CDS             9189..9401
                     /locus_tag="ACG81A_002959"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFS5016228.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9403..11082
                     /locus_tag="ACG81A_002960"
     CDS             9403..11082
                     /locus_tag="ACG81A_002960"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFS5016229.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11336..11536)
                     /locus_tag="ACG81A_002961"
     CDS             complement(11336..11536)
                     /locus_tag="ACG81A_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS5016230.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11686..11829)
                     /locus_tag="ACG81A_002962"
                     /pseudo
     CDS             complement(11686..11829)
                     /locus_tag="ACG81A_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            complement(12064..12726)
                     /locus_tag="ACG81A_002963"
     CDS             complement(12064..12726)
                     /locus_tag="ACG81A_002963"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS5016231.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            13118..13177
                     /locus_tag="ACG81A_002964"
     CDS             13118..13177
                     /locus_tag="ACG81A_002964"
                     /inference="COORDINATES: protein motif:HMM:NF019858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HFS5016232.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            13235..13966
                     /gene="erm(A)"
                     /locus_tag="ACG81A_002965"
     CDS             13235..13966
                     /gene="erm(A)"
                     /locus_tag="ACG81A_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HFS5016233.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
BASE COUNT         4183 a         2934 c         2375 g         4487 t
ORIGIN      
        1 gttttttctt aaccattttg aattggtttt tgcaaaccct ttaacttcat attcgtttgt
       61 ttcttccatg tacgcttcgt ccaaaaccat tgtttgtctt ctcaactcgc ttaatttctg
      121 tccttcgttg cttggcacag aaagtgtata aggggtcatt agttccatca gctgacgttt
      181 gattgcttcg atcaacgcca tcttaccttc tttcgtctga gtagaaatca atacatttgg
      241 gaatagcgta ggcgtgaaca tcgctgggtc gatctggtct gccttattgt aaacagtcag
      301 tatcggcatt tctttcatat tcagttcatc catcaatttt aagacagtct gctcctgtaa
      361 aatacgatct ggagaactag catcgactac atgtagcaaa agatccatat tttgactttc
      421 ttctaatgta gaatggaaag catcgatcag ttgcgtaggc aggtcttgga tgaacccgac
      481 ggtatccgtt acagtaatct caaatccttc agcaaaacgc catctcttag tcaatggatc
      541 taacgttgca aacaattgat ctttcgaata agtatccgct tgtgtcaata gattcaagat
      601 agttgacttc ccggcattcg tgtagccaat aagtccaatc tgaaaaatct cgctattttt
      661 tcttttttgg cgattgcgct ctcgatgagc ttctacttct ttcaattctt tcttaacagc
      721 aaaaatttta ttccgtatat gtcggcggtc tgtttctaac tttgtttcac ctggccctct
      781 tgttccgatc ccaccgccaa gacgagataa tgttttccct tgccctacta aacgaggcag
      841 caggtattct aactgagcaa gttcaacctg cagcttccct tctttcgaac gagcacgcat
      901 cgcaaaaata tctaagataa gctgcacgcg atcaattacc ggaagaccaa cagcatcggt
      961 aatcagttgg ctctgtctcg gagttagctc atgattaaag atgatcaaat ccgcttcatg
     1021 cgcatccgct tgttgaacta attcttgtaa tttcccttta ccgataatcg tttgccgatc
     1081 cacttgtggt ctcttttgtg taattgtgaa tacgacttct cctgatgcgg tattcgtcag
     1141 ctgaactagc tcttccattg actctgaaaa gtaacgctgg tttgcttcgg tttctacccc
     1201 tacgatgatc actttttctt ccattttatt ccgcctccag ccattttttg attgtttgct
     1261 caatctgact tttttgattt ggctcactta taagatcaaa ccactgtgca tccaaacggt
     1321 tacgaaacca cgtcaactga cgtttcgcgt atcttctcga atgtagtttg acttgttctg
     1381 ttgcttcttc caatgtttct ttcccttcaa aataagggaa aaattcttta tatccaatac
     1441 cttgactggc ctggacttct ccaagctcat acaccttacg cgcttctgct actaaacctt
     1501 ctgctagcat caaatcgact ctttgattga ttcgttcata aagaatacta cgcttcgtat
     1561 ctaatccaat catgaggtaa tcatacaatt gctttggctc ttctttaggt gccgtgatac
     1621 tatgtcctgt cgtctctaaa acttccagag ctcttatcac tttgcgttga ttgttccagt
     1681 ggattttctc agcagctgcc gggtcttttt ctttcaactg attccataat gcttgggaac
     1741 caattgtttc agctaacgtt tcatactttt ttctaatctc aggattcgct tcttctgtcg
     1801 ctcctaactg ataatcatat agaagggatt ggatatagag acccgtacca ccagctatga
     1861 taggtaggtg ccctcggttc gcaatctcgg taatcaattg tcgacctgaa tgctgaaaat
     1921 cagcggctga gtaacgttga gtaggttcga tcacatcaat caaatgatgc ggaacattat
     1981 tcatctcttc ttctttgatt tttgctgtac cgatatccag atttttatag atttgcaatg
     2041 aatcaccact gatgatctct cctccaaata gttgtgcgag ttccacactt agcgctgttt
     2101 taccgactgc tgtcggaccg acgatcacta atactttttc catattttac ctacttttcg
     2161 ttgattgttt ccaccgaaga gctttctccg gaaaatcagt tattattcca tctattttga
     2221 gttcttgaca tttggccatt tcttcttcat cgttaatcgt ccatgcacga attgcttttg
     2281 gaaaatcagc tgcctggact ggatgttcga acaaccaagt attttttgga tgtactcctt
     2341 ctatataagg ggtttctgct gctagttgga tctttttatc agaagtactc atcaataagt
     2401 ccagctgtat ctccggttcc agtaatgcga ttttttccaa agaatccaaa tgaaagctgc
     2461 aatatgcatg ttgaaaaggc caatctctac tttttaatgt ttcggctaat ttttcttcga
     2521 taccgggata ttcatactga tcagttttaa tttcaatcat tagcatccct cggtaatttt
     2581 tttggaggag cagtcctaac acttctctca attcaggaac tttagctgct ggaaattctt
     2641 tgctgaattt gccccctgca ttcaattttt tgagttcttc caatgttttt tctcgaatca
     2701 gccctttacc gttagtcgtc cggtccactt cttcgtcgtg cataacgatc aaataaccat
     2761 ctttgcttag atggacatct agttcgatac catctgcacc tactcgtaca gcttctgcaa
     2821 atgcaggaag cgtattttct gggtgggtgc cgctgcttcc gcgatgggca tagatttgta
     2881 acatctttct tcactccgtt cagttctatt gtatcattaa gaaaaaaaga aagcattacc
     2941 acgctctcat gtgcaacggc aatgcttcaa aatatcagct ccaaacatca tctttcgaga
     3001 agaaaccaat cggctggcgc gtcatagtct tcaactgagc ttttttttca ttgatcattt
     3061 cagtcagact atccgtaaaa ttctctaaaa tcacgcgtcc gcctttataa gcatatccac
     3121 caatcttcat tgtttcatct ggagataaaa tcacttttcg atgggagata ttcttaaata
     3181 gctcgtctaa aatataattt ggcagataaa aactattttg cgctgaaaat gattggagat
     3241 acgcaaaatc gtcaatcgtg atataacaat catcaaatgc atcaaagctg acatattctt
     3301 ccatcagcgt tgtccgtttt tgcaagatct cgttgatctt tttatttaat tgattcagcg
     3361 tctcgatttc tttgactttc aattccaatt cacgtctacg ctgttcttta ttttccaagt
     3421 gacgcatttg cagtatcatg ccgatataga caactacagc acctgtcagc actccgataa
     3481 ggccagtaat accaactatc aatctgttgt tcctccttgt tttgtttctc tgcttatcat
     3541 accataaagc gggtttatgc gactaatttg cctaagtttt ttatttgacc ttttttgacc
     3601 aaaaatgtat actacatttt aggtattaga aactaaatta tggtacacta tttctaaacg
     3661 aaatcaagga ggaactctat tatgaattta attcctacag tcattgaaca atcttcacga
     3721 ggtgaaagag cttacgatat ctactcacgt ttattgaaag accgtatcat catgttaagc
     3781 ggccaagtaa cagatgatct tgctaattcg atcatcgccc aattgctttt cttagacgct
     3841 caagattctg aaaaagatat ctatctttat atcaattctc ctggtggttc agttacagca
     3901 ggtatggcta tttacgacac aatgaacttt gtcaaagcag atgtccaaac aattgttatg
     3961 ggtatggctg cttcaatggg aagcttcttg ctgacagctg gtacaaaagg taaacgattt
     4021 gctttaccaa atgctgagat catgatccat caacctcttg gcggcgctca agggcaagca
     4081 actgaaatcg aaattgcggc acgccatatt ttgcaaacac gcgaacgctt aaacaaaatt
     4141 ttagctgaac gcactggaca accgcttgaa gtgattgaaa aagatacaga tcgtgacaac
     4201 tatatgacag ctgaacaagc aaaagcatat ggattgatcg atgaagtcat ggaaaacagt
     4261 tcttcattaa attaatctgt ttcaaaggaa gcttttttaa gctctcttat ctaaaccaaa
     4321 taaaaaagga tggtcaactc aaactgacca tcctttttta ttttatggat gctactgcct
     4381 atctttgctc cttgaatttt ccttgcgcat gcatccgacc tatctcgaca taatgctgga
     4441 tgttttcttt gtttttttgt atttcttctt ttgtcaaagg acggatcact ttagctgggc
     4501 gaccaaaagc caagacatta ggaggaataa cttttccttc tgttactaat gatccagcac
     4561 caatcaaact attctcacca ataaccgcat gattcaatat cgtggtcccc atcccgatca
     4621 gcgccccttt ttcgattgta cagccatgta gcatgcactg atgtcctacc gtcacattct
     4681 ccgcaatatc tacaggtgca tcatgatcga catggatgat tgtaccatct tggatattcg
     4741 ttctttgtcc tatttttatc caattactgt ccccgcgtaa aacagcttga taccagatcg
     4801 tcacatcttc tgacaatgtc acatcaccaa tcactgtggc atttgatgct ataaaccgtt
     4861 ccatatttat ttttctcctt tcattttttg tagaaactaa tcctattgaa taaaggtatc
     4921 atagcatata tttatttcat aagaaaaaaa acaggtaaaa cctgctcagg ccagatgtct
     4981 atttacagta tctggtaact gacgttttac ctgtttttat tgtacatcta tattttcttc
     5041 tattactttt tttaaaatta attcgtacac ttctatttgt gctgccaagc gttcgatttg
     5101 aaactcattt tcaaaaaaat cagctgaatc aaaaggcttc gtctgacaga cgtgcgttaa
     5161 acgatcaact tctcttttgg cttcagtaat tttttcattt aaaaactgtt ccatctccgt
     5221 gtcctcactt ttcttgataa ctttacttta ccattatcaa gaatagagaa caaaaaataa
     5281 gttttaaact gttttattct tatatgactt attctgcttc ctcagcaaat tggctgttat
     5341 acagtttttc ataaaatcca ccttgtgcca ataaactctc atgcgttcct ttttcaatga
     5401 tctgaccttg atccatcact agaatcagat ctgcatcacg gattgtcgat aaacggtgag
     5461 cgatgacgaa acttgtccgt ccctccatca cgcgatccat tgctttttga atcaacgctt
     5521 ctaaacgggt gtctactgaa cttgttgctt catctaaaat caagatttta ggatcggaga
     5581 ccactgctcg ggcaatcgtc agcaactgtt tttgtcctaa agacacattg tctccctcag
     5641 aattgatggc catatcatat ccatctggca tcgttcggat aaaatgatct acgtttgctg
     5701 ttttcgcagc atcaactact tcataatctg tcgcatcaag ctttccgaag cggatattat
     5761 ctgcaatcgt tccttgataa agccatgcat cttgcaagac cataccaaat agcgaacgca
     5821 catcactgcg actcattgat ttggtgtcga ttccatcaat ccttattgct cctttgtcta
     5881 cgtcataaaa acgcatcaat agattgatca atgtcgtctt gccggcaccg gtcggaccga
     5941 ccacagctac ggtttgacca gctttcactt caaaattcaa atcttggatt aatggtttct
     6001 tcggatcata tgcaaaatcg acatgatcga atgtcacatc tcctaaaatc gtttctggca
     6061 gtgaagcatc tactttgttt tctttttctt ctggctcatc taaaatctcg aatacacgtt
     6121 tcgttgccgc tgatgcactt tgcaaaactg aagacaattg tgtcacgttc cccattggct
     6181 gactgatttg ccaaatatat tggatgaatg cttgtagttg accgacaacg atcgccccag
     6241 taataacata aaagcttcct agaacagcca taccaatata agtagcatag gcagttaatt
     6301 gaactagagg catcatcaag ccagaaatga atgctgcacg aaacccaaag tatttcaagc
     6361 tgtgattaac ttttttgaat ccttccaatg tttctttttc gcgtccatat actttcaata
     6421 cactaaaacc tgtcatattt tcttggacgt agccgtttag ctcaccaaga gaattctgca
     6481 tgctttgaaa atatttctgc gaagcattga tcactcgttt tgagatccag atcgatgttg
     6541 gaatcatgat catagagatc agcgccatca tcggctggat ataaaacatc attgctgcag
     6601 ccaacgtaat tcccaatatc gcatttacga taccaataaa agcttgttgt aaagcaccgc
     6661 tgactgcatc tacatcattc gtcacacgag acaaaatatt tccttgctgg tttttatcga
     6721 aatatgaaac cggaagacga ttgatttttt cttctatgtc tcttctcaaa tcatgcatgg
     6781 cactttgaac gacatttgtc atcaaaacac cagaaagtaa ttgcgaaata caatacccta
     6841 ttccaacaac tgtgatccaa atgagacatt tgaatatata agaaaaattg attgattcgc
     6901 cattggcaat attacggctg atttctgtag taggaagtcc tgtaacatat ggcaaagcca
     6961 cgttgaaagc cactgtcaga actgtaaata ggatcactaa aagaaaagtc tcacgatacg
     7021 gtctgatata tcgagctaaa cgttttaagg aagaaaaggt ttttttcatg ctaattcctc
     7081 ctttgataat tgagaagcgg caatatcata ataaatcggg catttttcca acaactcccg
     7141 atgcgtcccc ataccgacga cttctccttc atttaatacg atgatcttat ctgcatgcat
     7201 gatcgttccc acacgttgtg ctacgatcaa tacagtagat tctgtcgttt cttttttcag
     7261 acgagcacgt aacttcgcat ccgtttgata atctaatgca gaaaagctgt catcaaaaat
     7321 atagatttcc ggacggcgta tgatggctct ggctattgcc aatcgttgtt tttgtccacc
     7381 tgaaaaattg gaaccacctt ctgataaatg ttcattaaat ccatctggtt ttcgagaaat
     7441 gaattctgaa gcttgagcaa tctcagctgc acgttccatt tcttcttgcg tagcatcttc
     7501 tttcccgtat cgcaaattat ctgcaatcgt tccagtaaat agcagcgctt tttgcggaat
     7561 aaacccaatc ttatctcgca acttgcttag ccgataatct ctcacgtcta ctccatcgat
     7621 caagatcttt ccttcactca catcataaaa gcgagggatc aattggatca acgtagattt
     7681 tccgctaccg gtacttccaa taaaagcaac ggtctctcct ggagaggctg taaaactgac
     7741 atttcgtatc actggctctt gcgaatggcc tggataagca aatgttacat tttgaaattc
     7801 gatataccct ttagtttttg tatcagatat accatttgga tcttctacaa tcgaaggttt
     7861 ggcatctagg gcttcttgga tacgtccagc tgaaacagct gctcttggat acatcatgaa
     7921 cacactagca aataacatga atgagaacaa ggcatggaat atatactcga taaacgcaat
     7981 caaatctcct acacgaagct cgcctgcatc gatttgcact gccccgctcc agatgatcaa
     8041 taccattacg atattaaata agaaataaaa acccggttgt gcaacagcca tcaaacggaa
     8101 taagcttttt gaactagaag cataattctc attgactaca gcaaaacgtt tttcttcaaa
     8161 cttttcattg acgaatgcac gaatcacacg caatcctgaa agattttccc gcaagatact
     8221 gttgatttta tctaagtttt tttgttgctt tttagataac ggttcagaca ccttagcaat
     8281 cgctacgact gccaataata gtaaaggtaa tgcacctact acatacaaac tgagtgtcac
     8341 acttgtacgc atgaccatgt acaaactgac aatgaacatc attggtgcca taaaaccgat
     8401 acgtaaaata ttttgcagga acaacatgat ctgataagca tcgtttgttg tacgagtgat
     8461 caatgaagat acgcctagat tttcgtattc ctgatgcgaa taactttgga tcttttcgaa
     8521 caaatcatct cgaatatctg ctacgatatt agtcgtaatc ctcgaaccaa aataacctaa
     8581 caagatattc aaaataatac cgattacagt aataacgatc ataaatatcc cttgttgctt
     8641 cacataatca aaatcatcat tgcggattcc tacatcgatc atacgtgcta aaatcgtagg
     8701 aagtcctaat tcaatcaaaa taaaaccaaa tacgcatatg aaattgaaaa acaagaattt
     8761 cttataacgc atggtataat gccacattag tttcactttc tcatctcctt ttaatctttt
     8821 gttacatttt cattactaaa aatgatagca caacgagcct attaatagaa cacttctctc
     8881 ataaaaaagt cattatgatt atagcgggat gataaacatt gattttttct ttcaatctag
     8941 ttgatttcta tacttatttg ctgttttatg ctagaataac aattaattgt tgtatatttt
     9001 ttatacagaa ggtacggttg aaaaaatatc cgaacagata agcaaaaacg aggcttgtct
     9061 ctttgactcg aatccagtat tctagtcaaa gcatatgaaa aaccaggtgg cttgtcccct
     9121 cgttttgtgt tgtctaaaaa aatcaactag ctaaatcact gctttacgca gataaaggag
     9181 acaaaacaat gatctataaa gtttattatc aagaaacaaa agtacgcaac cctaaacggg
     9241 aagaaacaaa atctctttac attgaggcaa aaagcgatgt agacgctcgc caacgtgtgg
     9301 aagaaaatac cccatacaat attgaattcg ttcaacttct agaaggaagt catctagagt
     9361 acgaaaaaga aaatgctgat ttctcattag tggagtttta atatgaaact ttccatcaaa
     9421 aataacgaga caggcgtctt tgctattggt ggattaggtg aaatcgggaa aaatacgtat
     9481 ggggtacaat tccaagatga aatcattttg attgatgccg ggatcaaatt cccggaagac
     9541 gatttacttg gaattgacta tgtcattccc gactacagct acatcataca aaacttacac
     9601 aaagtaaaag ccttggtgat tactcacggg cacgaagacc acatcggcgg aataccctac
     9661 ttgctgaaac aagcaaatat tcctatctat gctggtcctc tcgcattagc gttgatcaca
     9721 aacaaattag atgagcatgg cttgcttcga gatgcagagc tccatgagat caatgaagat
     9781 acgatcatcc gttttagaaa gacttctgtc agctttttca gaacgaccca tagtatccca
     9841 gatgcattag ggatcgtggt gaaaacacct tctggaaata tcgtagcgac tggagacttc
     9901 aaattcgatt tcactcctgt tggcgaacca gcaaacttgc ataaaatggc tcgtatcggg
     9961 gaagaaggag tcttgtgcct tttatctgat agtacaaatg ctgagattcc aacatttact
    10021 aaatcagaaa aaacgattgg gcattctatt ttgaagatct tttcaaaaat cgaaggacgt
    10081 atcatttttg ccagctttgc ttctaacatc ttccgtttgc agcaagcagc agatgccgct
    10141 gtggcaactg gtcgtaaaat cgctgttttc ggacgaagca tggaaaatgc catcgtcaac
    10201 ggtcaacgtt taggatatat caaagttcca gacggcacat ttgtagatgc acacgaaatc
    10261 aatcgcttgc ctgccaatga aacgatgatc ctatgtacag gttctcaagg agaagcgatg
    10321 gccgcgttga gccgcatcgc gaatggtact caccgtcaaa tccaaatcca gccagacgat
    10381 acagtgatct tttcaagttc acctattcct ggtaatacaa caagtgtcaa tcatttgatc
    10441 aatttgctgt ctgaagctgg tgcagaagtg attcacggaa aaatcaataa catccatact
    10501 tccggtcacg gtggacaaga agaacaaaaa ttaatgcttc gtttgatgaa gccaaaatat
    10561 tttattcctg tccacggaga attccgcatg ttgaaagtac atgcttctct agcacaagat
    10621 gtaggtgttc ctgcagaaaa ctgcttcatc atggaaaatg gcgatgtttt agcgttaact
    10681 agcgattctg cgcgtcctgc tgggcatttt gtggctggag atgtgtatat cgatggtagc
    10741 gggatcggtg atattggtaa tgtcgtttta cgtgaccgtc acctgctttc agaagaaggt
    10801 cttgtattag ctgtcgcgac agtggatatc aagaaaaaag aaatcttggc tggtccagat
    10861 attctctctc gtggatttgt ctacatgcga gaatcaggag aaatgatcaa tgaagctcag
    10921 cgaatcttat tccacgcttt aagagaatgt ttgaatggac ctgactgttc agaatataaa
    10981 ttgaaagaag cgatgactgg cgcgcttcag cctttcttat tcgaacgtac agaaagacat
    11041 ccaatgattt tgccaatggt aatgacacca gatgaagaat aaataagagg ttatgaaatc
    11101 agcaccttgt cctgaatata agagaaaaca aatgaaaaac atgagaagcc atttttgttt
    11161 gttcttattc cttgatacaa ggtggctctt tcatagcctc ttaaatttat ttactttttc
    11221 atacaaaaaa atacctgcca caagagaaat tgtctcttgt ggcaggtatt tttgttactc
    11281 gctttgttct ctcgtaaaaa ttgatgtcta ccatcaattt gatttacaga taaaattaag
    11341 cttttacaac gtttgcagct tgtgggccgc ggtctgattc ttcaacgtcg aaagtcactg
    11401 cttgaccttc ttctaatgtt ttgaatccgt caccttggat agctgagaag tgaacgaata
    11461 cgtcgcttcc atcttcacgt gagataaatc caaatccttt ttcagcgttg aaccatttta
    11521 ctgttccgtt ttccataata atatcctcct cgtgccaaaa gcactaattt aaattgtaac
    11581 attgctcgtg tacgatagag gtgcaacaga tgtttcaact tctattccca aacaaaatta
    11641 caatataatt gtacacttgc ttcatttttt ttgcaagaaa gcaacttact tctcttccat
    11701 cattccttct tctctcaaac taaaatatcg tttgcgtccg atgatcaagt ggtccagtac
    11761 agtgatcccc atcatttctc cacacttttg tatccttttg gtaaaattca tatcctgctg
    11821 cgatggaatt tcatattatc aagagtaaat tacaaaagaa tcatttaaat caatactttc
    11881 actttgaata catgtatttg aagtggaagg tacttatttc aaaaatagta aaacctgtat
    11941 cttaaattac ttaatagtaa cataagatac aggctgattt tttattcatt gttatttata
    12001 ctaaagcacc cgatagctct gaaaacaatc acaaattcaa cttttcaaag ccacagcttt
    12061 aagttatttt gtccagacaa cccccattgc ccaacccatt ttatggaatt ggcatccagg
    12121 caacaacttt tcatataaat cgtaataatt ttgttcagat aggtacttat ctgaagctaa
    12181 atgctcaagc catgatttag atgtgttgtg attataaatt ctaatcgcat tttttattcc
    12241 aaatttgaaa caatgcggta aaaactcttg gattgcccct aatttataaa cataggtagg
    12301 cggtgtctcg acttcagata cattatcaag aataactatt cttccttcct cattcaacag
    12361 ttccttcatt tgctgtatta cgctggctat atcatccaaa tgatgaaagg ttgttcggct
    12421 tacaataaaa tcaaacttct cattaaaatt aagttgttct gcattcatat tcagatagac
    12481 cgtatttgtt agttgacgtt tagatttggc aagatcgagc atttgattag aaatatcaat
    12541 ccctaccact tcatcataat aacttgctaa tttctccact aacaaacccg agccacatcc
    12601 gatatctaat gctctgcctt tctttggaga catattagac acaaagaatg aataatcatt
    12661 caaaagctca ttcacgaaat cgtaatcttc tgcaacctta tcaaactgtg attctattgt
    12721 attcaaaaag atcccccatt cctactttat cgacattctt tcattactta ccactttaga
    12781 tgttttttcg ttggggataa aacttccctt tagacaattt tatccaaaga caatacaaca
    12841 gtgcaacttt attaaagtca ctgtccttta tcgcagcctt tactttttag taaagacagt
    12901 ggcttctctt atcaagtttc aaaacatatt attttgaaga aaacgtccat ctgaagtgtc
    12961 aagtgcaaaa ttacatataa aggtttattc taaaatgaaa agatgataca atcatattca
    13021 gttacataag gaggtttcaa ttatgtgcac cagtatcgca gtagtagaaa ttactttatc
    13081 tcattcataa tgaaaaaaat ggaaaggaga taaaagtatg ggtacttttt ctatatttgt
    13141 tattaataaa gttcgttatc aaccaaatca aaattaattg gttataatga acgcttaatg
    13201 tcagttcatt ataaccagta aggagaaggt tataatgaac cagaaaaacc ctaaagacac
    13261 gcaaaatttt attacttcta aaaagcatgt aaaagaaata ttgaatcaca cgaatatcag
    13321 taaacaagac aacgtaatag aaatcggatc aggaaaagga cattttacca aagagctagt
    13381 caaaatgagt cgatcagtta ctgctataga aattgatgga ggcttatgtc aagtgactaa
    13441 agaagcggta aacccctctg agaatataaa agtgattcaa acggatattc taaaattttc
    13501 cttcccaaaa catataaact ataagatata tggtaatatt ccttataaca tcagtacgga
    13561 tattgtcaaa agaattacct ttgaaagtca ggctaaatat agctatctta tcgttgagaa
    13621 gggatttgcg aaaagattgc aaaatctgca acgagctttg ggtttactat taatggtgga
    13681 gatggatata aaaatgctca aaaaagtacc accactatat tttcatccta agccaagtgt
    13741 agactctgta ttgattgttc ttgaacgaca tcaaccattg atttcaaaga aggactacaa
    13801 aaagtatcga tcttttgttt ataagtgggt aaaccgtgaa tatcgtgttc ttttcactaa
    13861 aaaccaattc cgacaggctt tgaagcatgc aaatgtcact aatattaata aactatcgaa
    13921 ggaacaattt ctttctattt tcaatagtta caaattgttt cactaaatta aagtaataa
//
LOCUS       DAZULY010000480         5614 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.guided.171, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000480 DAZULY010000000
VERSION     DAZULY010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.guided.171"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..1027)
                     /locus_tag="ACG81A_002966"
                     /pseudo
     CDS             complement(41..1027)
                     /locus_tag="ACG81A_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1179..1874
                     /gene="vanR-A"
                     /locus_tag="ACG81A_002967"
     CDS             1179..1874
                     /gene="vanR-A"
                     /locus_tag="ACG81A_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFS5016234.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1852..3006
                     /locus_tag="ACG81A_002968"
     CDS             1852..3006
                     /locus_tag="ACG81A_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFS5016235.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3221..4189
                     /locus_tag="ACG81A_002969"
     CDS             3221..4189
                     /locus_tag="ACG81A_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFS5016236.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4182..5213
                     /gene="vanA"
                     /locus_tag="ACG81A_002970"
     CDS             4182..5213
                     /gene="vanA"
                     /locus_tag="ACG81A_002970"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFS5016237.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5219..5584
                     /locus_tag="ACG81A_002971"
                     /pseudo
     CDS             5219..5584
                     /locus_tag="ACG81A_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
BASE COUNT         1697 a         1091 c         1263 g         1563 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccct atttccgata accgtgtatt
       61 gaaaggcatc ttcgaagcca ttatccaata tttcgcaggc ttttgtatat ttaggctggt
      121 cgatatattc accgactaag gcattcttag ctgttcgagc gagttcaata tccgtaaact
      181 taaagatcgc ttttactgct tctctaaacg gttttgaatt cttttttgga atggaactga
      241 agatgtttct taaaaaatgg acctggcatc tctgccaact tgcgttggta aatgacttac
      301 gaatcgcaga cactaggcct ttatgggcat cagaaatgat gagttctgtc ccctttaggc
      361 cgcgttcttt taagtattca aagaagatgg accatgtgtc atcactttct tcattttgaa
      421 tcatgaagcc aatgatttca cggtcgccac cttctgttat cccgatcgca atatggcagc
      481 ttttagaaag cactcgatgg tcctcacgga cttttatgta gagaacatca gtcatcagat
      541 aaggataatt cgtacctgag agtgaacggt tctgccattc gttgaccatc gggtctaact
      601 gctcagtcag gctagaaaca aaagatttcg atacagattt tccacatagc tcttcaacaa
      661 tctttgaaac tttacgtgtc gaaacgcccg aaacatacat ctcaagcatt gaagcgagca
      721 gtgccttttc atttcgctga taacgctcaa acaccgtcgg tgaaaattca ccatcacgtg
      781 ttctaggcac ttttaattcg agtgtaccca cacgagtcgt aaagtctcgc tcataatagc
      841 catttctttg actctgacga ctttctgaac gttcatagtc atcggcttga atatattctg
      901 ttcgttgatt ttccatcaat tggttgaaaa cagtggttaa aatattttta gaaacatcat
      961 cttttaccga atgttcaata atactttgaa cctcttcgtt gttcagtgta aaatgtactt
     1021 gggtcatgta acgtcctcct gggtatgttt ttgtggttaa aaacattgta ccgtaaaagg
     1081 gctgttacat ggccttttat cttttacaca attatatgga ctttatccta taatctgata
     1141 aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg
     1201 tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata
     1261 cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg
     1321 accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa
     1381 taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata
     1441 aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg
     1501 agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg
     1561 agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt
     1621 gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc
     1681 tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg
     1741 acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa
     1801 aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata
     1861 aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc
     1921 gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg
     1981 aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac
     2041 gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg
     2101 attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt
     2161 gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt
     2221 tctgcggaaa tggatgttat ggaacaaaag ctcaacacat taaaacggac tctggaaaag
     2281 cgagagcagg atgcaaagct ggccgaacaa agaaaaaatg acgttgttat gtacttggcg
     2341 cacgatatta aaacgcccct tacatccatt atcggttatt tgagcctgct tgacgaggct
     2401 ccagacatgc cggtagatca aaaggcaaag tatgtgcata tcacgttgga caaagcgtat
     2461 cgactcgaac agctaatcga cgagtttttt gagattacac ggtataacct acaaacgata
     2521 acgctaacaa aaacgcacat agacctatac tatatgctgg tgcagatgac cgatgaattt
     2581 tatcctcagc tttccgcaca tggaaaacag gcggttattc acgcccccga ggatctgacc
     2641 gtgtccggcg accctgataa actcgcgaga gtctttaaca acattttgaa aaacgccgct
     2701 gcatacagtg aggataacag catcattgac attaccgcgg gcctctccgg ggatgtggtg
     2761 tcaatcgaat tcaagaacac tggaagcatc ccaaaagata agctagctgc catatttgaa
     2821 aagttctata ggctggacaa tgctcgttct tccgatacgg gtggcgcggg acttggattg
     2881 gcgattgcaa aagaaattat tgttcagcat ggagggcaga tttacgcgga aagcaatgat
     2941 aactatacga cgtttagggt agagcttcca gcgatgccag acttggttga taaaaggagg
     3001 tcctaagaga tgtatataat tttttaggaa aatctcaagg ttatctttac tttttcttag
     3061 gaaattaaca atttaatatt aagaaacggc tcgttcttac acggtagact taataccgta
     3121 agaacgagcc gttttcgttc ttcagagaaa gatttgacaa gattaccatt ggcatccccg
     3181 ttttatttgg tgcctttcac agaaagggtt ggtcttaatt atgaataaca tcggcattac
     3241 tgtttatgga tgtgagcagg atgaggcaga tgcattccat gctctttcgc ctcgctttgg
     3301 cgttatggca acgataatta acgccaacgt gtcggaatcc aacgccaaat ccgcgccttt
     3361 caatcaatgt atcagtgtgg gacataaatc agagatttcc gcctctattc ttcttgcgct
     3421 gaagagagcc ggtgtgaaat atatttctac ccgaagcatc ggctgcaatc atatagatac
     3481 aactgctgct aagagaatgg gcatcactgt cgacaatgtg gcgtactcgc cggatagcgt
     3541 tgccgattat actatgatgc taattcttat ggcagtacgc aacgtaaaat cgattgtgcg
     3601 ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt ggcaaggtac tcagcgacat
     3661 gacagttggt gtggtgggaa cgggccagat aggcaaagcg gttattgagc ggctgcgagg
     3721 atttggatgt aaagtgttgg cttatagtcg cagccgaagt atagaggtaa actatgtacc
     3781 gtttgatgag ttgctgcaaa atagcgatat cgttacgctt catgtgccgc tcaatacgga
     3841 tacgcactat attatcagcc acgaacaaat acagagaatg aagcaaggag catttcttat
     3901 caatactggg cgcggtccac ttgtagatac ctatgagttg gttaaagcat tagaaaacgg
     3961 gaaactgggc ggtgccgcat tggatgtatt ggaaggagag gaagagtttt tctactctga
     4021 ttgcacccaa aaaccaattg ataatcaatt tttacttaaa cttcaaagaa tgcctaacgt
     4081 gataatcaca ccgcatacgg cctattatac cgagcaagcg ttgcgtgata ccgttgaaaa
     4141 aaccattaaa aactgtttgg attttgaaag gagacaggag catgaataga ataaaagttg
     4201 caatactgtt tgggggttgc tcagaggagc atgacgtatc ggtaaaatct gcaatagaga
     4261 tagccgctaa cattaataaa gaaaaatacg agccgttata cattggaatt acgaaatctg
     4321 gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat tgctattcag
     4381 ctgtactctc gccggataaa aaaatgcacg gattacttgt taaaaagaac catgaatatg
     4441 aaatcaacca tgttgatgta gcattttcag ctttgcatgg caagtcaggt gaagatggat
     4501 ccatacaagg tctgtttgaa ttgtccggta tcccttttgt aggctgcgat attcaaagct
     4561 cagcaatttg tatggacaaa tcgttgacat acatcgttgc gaaaaatgct gggatagcta
     4621 ctcccgcctt ttgggttatt aataaagatg ataggccggt ggcagctacg tttacctatc
     4681 ctgtttttgt taagccggcg cgttcaggct catccttcgg tgtgaaaaaa gtcaatagcg
     4741 cggacgaatt ggactacgca attgaatcgg caagacaata tgacagcaaa atcttaattg
     4801 agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt gccgcgttag
     4861 ttgttggcga ggtggaccaa atcaggctgc agtacggaat ctttcgtatt catcaggaag
     4921 tcgagccgga aaaaggctct gaaaacgcag ttataaccgt tcccgcagac ctttcagcag
     4981 aggagcgagg acggatacag gaaacggcaa aaaaaatata taaagcgctc ggctgtagag
     5041 gtctagcccg tgtggatatg tttttacaag ataacggccg cattgtactg aacgaagtca
     5101 atactctgcc cggtttcacg tcatacagtc gttatccccg tatgatggcc gctgcaggta
     5161 ttgcacttcc cgaactgatt gaccgcttga tcgtattagc gttaaagggg tgataagcat
     5221 ggaaatagga tttacttttt tagatgaaat agtacacggt gttcgttggg acgctaaata
     5281 tgccacttgg gataatttca ccggaaaacc ggttgacggt tatgaagtaa atcgcattgt
     5341 agggacatac gagttggctg aatcgctttt gaaggcaaaa gaactggctg ctacccaagg
     5401 gtacggattg cttctatggg acggttaccg tcctaagcgt gctgtaaact gttttatgca
     5461 atgggctgca cagccggaaa ataacctgac aaaggaaagt tattatccca atattgaccg
     5521 aactgagatg atttcaaaag gatacgtggc ttcaaaatca agccatagcc gcggcggttc
     5581 tgttgcaaag ttttaaataa agaataaaat ccct
//
LOCUS       DAZULY010000481         4341 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.guided.212, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000481 DAZULY010000000
VERSION     DAZULY010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.guided.212"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            291..1769
                     /gene="msr(C)"
                     /locus_tag="ACG81A_002972"
     CDS             291..1769
                     /gene="msr(C)"
                     /locus_tag="ACG81A_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFS5016238.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1816..2262
                     /locus_tag="ACG81A_002973"
     CDS             1816..2262
                     /locus_tag="ACG81A_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFS5016239.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2461..2976)
                     /locus_tag="ACG81A_002974"
     CDS             complement(2461..2976)
                     /locus_tag="ACG81A_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFS5016240.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            3093..4295
                     /locus_tag="ACG81A_002975"
     CDS             3093..4295
                     /locus_tag="ACG81A_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS5016241.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1307 a          728 c          941 g         1365 t
ORIGIN      
        1 aaaaggaggt cggaaaaatg actgcatcga tgaaattacg tttcgaactt ttgaataaca
       61 actaaataaa ttcaaaagac atgtataaac aacaatttca tggatggagt atgtccattt
      121 tttcgaacga cagtagaata ggtgaaagct atgacagatt tggtgattta gtcatagtta
      181 tttcgttcag agtaggaaaa gatgtgatgc agtttttttg caaacagatt tttctgctct
      241 tttttagtat cgataattgt tgttttacat gaaataggag tgtaaaacat atggaaaatt
      301 tagcagtaaa tataacaaat ctacaagtca gctttggcaa ccagctagaa ctatctattg
      361 attctcttcg tgtctatcag caagatcgta tagggatcat aggagaaaat ggtgtcggta
      421 aatcgacttt gctcaaacta atagccggtg aactttttcc cgatcatggg aaaatccaaa
      481 cagagatcac cttcaactac ctgcctcaat taacctatct cgctgaagca aaggacctaa
      541 atttggaatt agccagtcat ttccagttaa gactggaaga aacttcagag cggaaatgga
      601 gcggagggga agaacgaaag atcgagttga tacgtcttct ttcttcttat gaacaaggga
      661 tgcttctaga cgagccaaca acccatctag atagaaaaag tattgatcga ctgattgaag
      721 agcttcgtta ttattatggt acgctggttt ttgttagtca tgatcgctat tttctagatg
      781 agctagcatc gaaaatctgg gaagtaaaag acggagaaat ccgagagttt tcggggaatt
      841 atagtgccta tctcactcaa aaggaattgg agaaaaagac tcagctacga gaagcagagt
      901 cgatcatgaa agagaaaaaa cgattggaaa aatcgatcca agaaaagaaa aaacaagcgg
      961 aaaagttgga aaaagtgtcc agtaaaaaga aaaagcaaca aatcagactg gatcggttgt
     1021 cttcctctaa acaaaaagac agtgtacaaa aagccatcca aaagaatgcg aaaacattag
     1081 agagaagact ccaaaaaata ggagaaacaa ccaagccgca gcagatgaaa caaatccgtt
     1141 ttccagtacc aaaatctctt gaactccaca gccgctatcc catcatggga caaaatgtcc
     1201 aattggaaag aagtgggaga acattactgg taaatggtga ttttcagttt tctttaggta
     1261 aaaaaatcgc gattgtcggt gaaaatggtt caggtaagac aaccttatta gaacatatcc
     1321 gcaaacaagg agaaggaatc cttctctctc cgaaagtaag ctttcaagta tatcagcaaa
     1381 agggttatca aatgacatct gaagaatcca tcattcgttt tgtcatgaga caaacagagt
     1441 tttcagaatc gcttgtccgt agtttgctga atcacttagg gtttgctcag gaaactttga
     1501 cgaaaccgtt atgtacatta agtgggggag aagcgacccg tctgacgatt gctttgcttt
     1561 ttactaagcc aagtaatgtg ttgctgttag atgaaccgac taattttatt gatatggcaa
     1621 cgatcgaagc tttagagaag ctaatgcaaa tatatccggg aacgattttg tttacttctc
     1681 atgattctta ctttgtcgag cgtacggctg atgaagttta tgaaataaaa gggcagaaaa
     1741 taaaaaaagt acttacgaga aatttttaaa tgtgctacga tgattttcgt gacaaattta
     1801 aaggagtggt acagattgcc tacaaataaa aaagaaggaa taatttttac aacaatgatg
     1861 tgttttttga tggtcttggg gatgagcgcg tataatttat ggctgcatca agatcttcat
     1921 ttatcaagtt tgttgattgg tctagtgcct ggattcgtgg tggctttcat tttggatgtc
     1981 ttcatcatcg gtgtgctggc gaaaaaaatt gcctttaaac taccagtaaa tccagaaagc
     2041 aaggtccaaa tgatcttaac tatttcatgc ttgatggttt taggaatggt gacatgtatg
     2101 tctttattcg gtgtgatcat ggaacaagga atacctgatc aattattctc tgcttatatg
     2161 aatgcttgga aaatgaatgt gattgccgct ttaccgttgc agttgatcat cgttggcccg
     2221 ctttcaagaa gtgtgctaaa agcaattcaa agtaatgaat gatggaacac tgttatttga
     2281 aaaaaggcct tggcatgctt tttgtcatag tctcttgaat gcagttctag aaaaatagtg
     2341 atccgaacga taggttttgg cctgtcgttc ggatcgcttt ttcttttcca actgtattgc
     2401 tcttttattg aagataattt gctcttcaat agatatttct agaaaggttc tgtttatccc
     2461 ttactcaaaa aactgctgca tttgttctct tacttgctga caccattgga gttcctgctg
     2521 acaaagcata attctgcgct ggatcacaac ataacgtcca aattcttgtt tcttgtctag
     2581 cgagaggtca ttcaattttt tttgattttt ttgcatgatt ttgttcagct gttgttctct
     2641 agtgaatagc agtccagtag ctgttgtttt atcgagcgtc gaaaaagcat agatttttgc
     2701 tagaaattca tctttttgcg taggagcggg agtttcctct ttgatccatt cctctacaag
     2761 agcacgtcct tcatcagtca agtggtagat ctttttttga gagtgctcgt ctttgtccaa
     2821 aacggtcaca taaccctgct ggtctaactt ggcaagcgtt gtatagatct gactgtgatg
     2881 ggcttcccaa aaaattgtga tcagctgctt caattcatac ccagaacgtg gtttggataa
     2941 aagcacgcat aataatatat aggctaaagt attcataaaa atcctacctc tcgtcttatt
     3001 ttctttatta tatcacagta aagtttccac gttttacatg tgcatattga catgtaaaat
     3061 aaatcatgta tgatgctaat agagagaaag ggatggaaat attggagaat caaatagata
     3121 tacaagaaca aacaaatgaa gaaacaaagg ggaaaagttc agcgctagtt gtagcagcta
     3181 cctgtatgat cgcttttatg ggagtgggtc tagtagaccc aatcttaaaa acgattgctg
     3241 cacagttaaa cgctacgcca gctgaaacga ccctgttatt tactagttac atgttagtca
     3301 ctggtgtggt gatgctattt acaggtttct tatctagtcg gatcggtttg aaaaaaacat
     3361 tgatgacagg attgtttatc atcgttgtat tcgctggtct tggcggatta tctgggaata
     3421 tcggaatgct gattgggtta cgtgccggct ggggtgtcgg gaatgcttta tttgtctcta
     3481 ctgctttagc aacgatcgtc agtattttag ttggtaacac agaaaaagca atcatgatgt
     3541 atgaagcagc acttggctgc ggaatggctg ttggccctct agtcggtggg gtcttgggca
     3601 gtggttcatg gcgttatcca ttttttgggg tagcattttt gatgttttgc ggttttatag
     3661 ccctagcggt acttttacct gaaactgaga aaccaaagag aaaagtaggc ctttgggaag
     3721 gtgtcaaagc gttaagcaat cataaacttc gttcagttgg actaatcgca ttgttatata
     3781 atttcggatt ctttacttta cttgcgtatg cgccattttt attgattggc tattctgaat
     3841 tagaagtcgg ttttgtattt tttggctggg gtgtgcttct ggctattgct tcgattttcg
     3901 ttgcaccggc tcttgagcgg aagtttacga cttatcggac aattttagca gcatttgtct
     3961 tattcattat ttgtttgctg ttattaggag ctggaacttc tatgccagtc cttgtaccga
     4021 tcagcattgt gttagcagga tttttccaag gaattatcaa tacactgttg acgacgattg
     4081 ctatggaaat accaggtctg caacgaaacg ttgcttcgtc cagctatagt tttgtccgct
     4141 ttttcggtgg agctttagca ccatttattg caggtgtgat tggtgaacat attggtgaaa
     4201 gctatgcgtt ttattttgca ggtgccattg ttcttatcag tgtatgttgt atcatttatc
     4261 accgtaatta ttttgctaca aatgaagaag aataagaaat cagaggttgt gaaaaagctg
     4321 tcctgcatca agtcataaga a
//
LOCUS       DAZULY010000482         3589 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.guided.238, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000482 DAZULY010000000
VERSION     DAZULY010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.guided.238"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            58..1179
                     /locus_tag="ACG81A_002976"
     CDS             58..1179
                     /locus_tag="ACG81A_002976"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS5016242.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1437..2939
                     /gene="eat(A)"
                     /locus_tag="ACG81A_002977"
     CDS             1437..2939
                     /gene="eat(A)"
                     /locus_tag="ACG81A_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFS5016243.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            3006..3533
                     /locus_tag="ACG81A_002978"
     CDS             3006..3533
                     /locus_tag="ACG81A_002978"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFS5016244.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1223 a          618 c          806 g          942 t
ORIGIN      
        1 tttgtttgaa aaaaatgaaa aaaagtgtct tacttaagaa gaaagtgagg gatacagatg
       61 gatattttgg cggcaattga ttcatttaaa ggaagtgctt cttcatttga actaaatcag
      121 gcagttctaa atggctggga agatcgtaaa ggaagaaaaa tcaatgtgcc gattgcagat
      181 ggcggagaaa atactgcaga agcaatctat tacgcgctca ggggaacatg gcgtaccatt
      241 gatgggatgg atctcttatt ccgtcagcga aaaatcagct atttattgac aacttggcaa
      301 ggaaaaaaca ccgcagtcat cgaatctgca caagcattgg ggttagatct acttgataaa
      361 ccaacggatc aaactatccg acttgcttcc agttatgcct taggagaact tattaaagat
      421 gcactgcttc aagaagtaga acaaattatt gtcactttag gaggaagcgg gtgtagcgat
      481 ggtggtctag gattactcca atccttgggt gcttctttag aagggtacac agaaggaaat
      541 cctttattat ctactaaaaa acttcatttt gatcgaagaa acgttcttgt ttctaaagta
      601 aaattgatta tcgctgcaga tgttaccaat ccatatacag gtcaacaagg agctgcttgg
      661 caatttggaa gacaaaaagg aggaatagat acgacgctag ctttcttaga taaacaagca
      721 ggaaagattg ctcagcaaat aaaagatttc tgggaggtgg atttagatga tattccagga
      781 agtggagcag caggcggtat tggtggagcc ctttttttgt tgggagcgaa gatggtttct
      841 ggtttcgatt tagtcagcca aatcactgga ttggaaaaaa agatcgaaca agcagatgtc
      901 atcattacag gagaagggcg gatcgatcag caaacgatcc acggaaaagt tccatacggt
      961 gtagcagtgc ttgcaaaaaa atatcgaaaa ccaataatcg ctatttgcgg aagtagagac
     1021 caagagctag aaggactttc tgattttctc ccaattgtct taagtattca tttgggtcca
     1081 ataggtttag aagaagcaat ggaacacagg caaacattag aaaaaacaaa aatcttagga
     1141 cagacactta gcagattttt caccatcctc ccgaagtagt tgacaaaatc attttcttta
     1201 tgctatcgta atttcaaata taggacaaag tgagggatcc gtgcattgaa aaattaagtc
     1261 gattgatgaa agctgcattc agactgtacc ttgtggagac aaggaagcag tgtgcagatt
     1321 ttcgggattg gcttggtttt ttcgatgtat gggtcttttt gtgtgtatcc aacccttgca
     1381 tcctctttag ccagttctga aaataggact ggtctttttt tacgaggtga tggaaaatgt
     1441 ctaaaatcga aataaaaaat ctgacattcg gctacgacag ccaaggcaca ttattatttg
     1501 aacaagcaaa tctaaatttt gacacacaat ggaaactagg acttatcgga cgaaacggtc
     1561 gaggaaagac aactttactg aatattctac aaaacaaact accttatcaa gggcaagtaa
     1621 tccatcagca agaatttgcc tatttcccgc aacagacaaa agataaagaa cgtttaacct
     1681 attacgtgtt aaatgatatt acggattttg agatatggga aatcgaaaga gagctccaat
     1741 tgatgcaaac agatcctgaa atcttatgga gagaattcag cacactatcg gggggagaga
     1801 agacaaaagt cctactggca cttttatttg tggatgacac tcatttcccg ttaatcgatg
     1861 aaccaacgaa tcatttggat atctctggta gaaaacaagt agcggcttat ttgaaaaaga
     1921 aaaaacaagg cttcatcgtg gtcagccatg accggggatt tatcgatgaa gtagtggacc
     1981 atgttttagc aatcgaaaaa agtcaactgg aactttatca agggaatttc tctatctatg
     2041 aagaacagaa aaaacttcgt gatgaatttg aaatggctca aaatgaaaaa ttgaaaaaag
     2101 aagtcagtag gctaaagaaa acagcagctg aaaaagccga atggtctcgt tcccgagaag
     2161 gagataaaac aaagaaacaa gtcggattca tcgatactga atctagacga gtgaataaag
     2221 gagcagtggg tgctgatgct gcacggacga tgaaacgatc caaagcaatc gtgaatcgga
     2281 tggagaccca gatcagcgag aaagaaaaac tattaaaaga tatcgaatat atcgattctt
     2341 tgacgatgaa tagccaagcg tctcaccata agcgactttt aagcgtagaa gatcttcaat
     2401 tagggtatga aaatctgtta ttcgagccaa ttcattttac aatcgagcct catcagcggg
     2461 tggcgatttc aggtcctaac ggtgcaggaa agtcatccat tatccattat cttctggggg
     2521 cattcaacgg caaggttata ggagaaaaat accagccaaa acatctgagc attagttatg
     2581 caagccaaaa ttatgaagac aatcgaggaa cgttggcgga atttgcagag aaaaaccaag
     2641 tagactacca agcatttttg aacaacctcc gaaagcttgg gatggaaaga gatgtttttc
     2701 ataacaagat cgagcagatg agtatgggcc aacggaaaaa agtggaattg gctaaatctt
     2761 tatcacagcc agctgaacta tatacatggg atgaaccatt gaattatttg gatgtcttca
     2821 atcaagaaca attagaacaa ctgatcttga acgtgaaacc tgccatgtta ctagtggaac
     2881 atgatcaaac cttcctggat aaagtatcta ctgagattat ttctcttgag agaatctaac
     2941 ccttgtaatc tcaagaacaa caaggtatgg ttaaggggaa ctttgccaac gaaggagaga
     3001 atcacatgct ttatgtaacg agacacggag aaacaacttg gaacgcacaa ggattagttt
     3061 gcggacgggc agatgtgccg ctgactgaaa aaggacagat gcaggcacag aaactagcag
     3121 aaaaggtagt agatttgcct gttccaatca cgaaaatcat ccattcccct cttcaacgag
     3181 caagagatac agcacaagca gttgctgatc gtctcagctt gccgctgacc gttgatgaac
     3241 gattggtaga aatggatttt ggggattatg atggactgcc aagcaaagat gagaactttc
     3301 aaaaagcacg tttagcgttc gctgtacgat tcccaaatgg ggagtcagtc ttagatgtgt
     3361 atgcacggat cgttccttta ttgaaagaat gcatcgaaga cgaagaaaat gtctatctgt
     3421 tagtttgcca taatgccttg ataagagtga tcaatgctta ttttcatccg atgcctaatg
     3481 aggggttctt tacattcatg gtagataata cagaattggt ttcttatgag taattatgtt
     3541 tttgtgttat ttaaataaaa tatttcaagg cactgctttt gcttagcag
//
LOCUS       DAZULY010000483         2956 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.guided.257, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000483 DAZULY010000000
VERSION     DAZULY010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.guided.257"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            120..617
                     /gene="dfrG"
                     /locus_tag="ACG81A_002979"
     CDS             120..617
                     /gene="dfrG"
                     /locus_tag="ACG81A_002979"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFS5016245.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(689..2641)
                     /locus_tag="ACG81A_002980"
     CDS             complement(689..2641)
                     /locus_tag="ACG81A_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFS5016246.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2648..2884)
                     /locus_tag="ACG81A_002981"
     CDS             complement(2648..2884)
                     /locus_tag="ACG81A_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016247.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT          897 a          525 c          500 g         1034 t
ORIGIN      
        1 tgataatata acttatagaa aaaattgctt attcaactaa cgggcaggat aatgagaaaa
       61 ggttttttga tagaaataag aaattagatg gtaaattagt taaaaaggct gtgttataaa
      121 tgaaagtttc tttgattgct gcgatggata agaatagagt gattggcaaa gagaatgaca
      181 ttccttggag gattcccaag gactgggaat atgttaaaaa tactacaaag ggacatccga
      241 taatattagg taggaagaac cttgaatcaa tcggaagagc cttacctgac agaagaaata
      301 ttattctgac gagagataag gggtttacct ttaatggttg tgaaattgtt cattcaatag
      361 aagatgtttt tgagttatgt aaaaacgaag aagaaatttt tattttcgga ggagaacaga
      421 tttataattt gtttttccct tatgttgaga aaatgtacat cacaaaaata catcatgaat
      481 tcgaaggaga tacttttttt ccagaagtga attatgagga atggaatgag gtatttgccc
      541 aaaaagggat aaagaatgat aaaaatccgt ataactacta ttttcatgta tatgaaagaa
      601 aaaacttatt gagttaacgg gtgcgttaat tgaagaagaa cagtaaagat aaagcacaag
      661 agcttgggat tactccaagc tctttttgtc atttgaaata aattatatct ttccaatcaa
      721 attgtttatc tgtaatccct aatctttgag caggtgtcaa aaccttttta tccgctgatt
      781 taaaagggcg acaaaagttg tagtaagttc ttagaattgt aatcgcaaat tgagcatact
      841 tcggattgaa gttagcgtaa atatagctct tcttatcacc acgagctgtt gttaatggtc
      901 tttcaagaga tgatatataa cgtcttatct gttgaataaa gctattggtc gaatggtcat
      961 taacatttaa tagcatcttt gcgatttcgt ttggttccag agcagataaa tccgttctac
     1021 aatcaactga ataaaatcct ttatccttag tggctaatgg atgtttgata gggttatcgg
     1081 catatttgcg atatctttct ccatctttac taatagcctc ttcgtggaat tgatgacttg
     1141 ttagtaattc agttagataa tgagatgcca aagtccgtag aaactttgtt ttgaatcctc
     1201 tgatatctcc ccaatcgagc agttctgctt ttgcttgtcc gaattctttt aagcattgtt
     1261 ttctgctttt cgtcttgtta acttggcaaa taaaatgatg tgcatcgctc aaccttaatt
     1321 ctttcgtgaa tactctataa aaggcgtttc taatagagaa gtcatcatca ctaatcattc
     1381 gccattcgtc ggcattaacc atttgtttaa ttaaccaata gtgagcagtt gtagtataag
     1441 gagcatcaac atttaaacca tcgacaaatc tactcctatt agtaattttc cctaattcgt
     1501 gaagataagc attcctattt tcgctatcgt tagcggatgg ttcttgtgga taataagacc
     1561 aatctaacct atcattttta cgactaaaag tattgagatg gtcttctttg aattttcttg
     1621 tatcttcatt taattcatcc attgaaatat tccaatcata ggcaacatca gaacggaaca
     1681 catatcttga taagacctca gcagatacaa ctatatacgt ttgcatatta aggtcttcaa
     1741 accccgtata tttctttgaa ccttgtcctt tcttacggac gttatttagg tagtagtgca
     1801 tcttgtccgt attaagccac atctcgttaa atttctttgt ttgaaggggt tgtgtctcat
     1861 atcgttctaa aaactccaaa caacgcctat acagccactc cagcttatga tagtaggttc
     1921 caactccaat tcctaaaata tcacaagtac gactgactga aacccttcca acaaccatct
     1981 tagcgaacat tggaagaatg gtgttctttt gttggtgata agtagtggtt tcttctcttt
     2041 ttggtagaac attagtgaac ttcttacacg ccttacattg atagcgttga gatttgcccc
     2101 tacttttccc ccttttgtaa aattgcttcg gctcattgaa tggggttgat tcttcttccg
     2161 agcagccttc tttatgaaat tgataatctg gctcaacatc ttggatgcta ttgatttcta
     2221 taaggcgttt aatttcttca acaacagacc aattagataa aggggttact gtacagtttt
     2281 gcgatacacc tctgccaata ggattgtcat tgcagtacat acctttatga cctttatctt
     2341 tggaactacc agtcatttta tatcgacttg gtttcccttt tacatctttg aatttgtgct
     2401 gttctttccc aaaattacaa caaaacggat tgatgcagta attatattga atgggatatt
     2461 cagaaccgtt ccatttaaaa gacgttggca agaacaacat tttgttatac cttgttgcaa
     2521 atcgttttgg agttaataaa tcatattgtt tagccctatc ttttaattct tcgtttgaaa
     2581 tcggaatttt aacatctaca atttcatctt ccttcgtggc taatctttta agcttggtca
     2641 ttgtctatca acccactcta tctttgatac ccattttttc aacaatccgc ttattacttc
     2701 gcttgttggc aatccattca ataaacttct tatcgtctaa aatgttcaat aagaattcat
     2761 ccactgcttc gctttcagta cgctctgcat attcagcgta ttgcttaact atcaccctta
     2821 cttgctctga tatttcccaa tccacagact tagcgttctt attttttggc tcaagaaatt
     2881 gcataggaaa atgcacccct ttttgcttag taaattgtaa ccattttaac acaccaattt
     2941 gcattcctac aataat
//
LOCUS       DAZULY010000484         1937 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.guided.312, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000484 DAZULY010000000
VERSION     DAZULY010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.guided.312"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            16..365
                     /locus_tag="ACG81A_002982"
                     /pseudo
     CDS             16..365
                     /locus_tag="ACG81A_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(637..927)
                     /locus_tag="ACG81A_002983"
     CDS             complement(637..927)
                     /locus_tag="ACG81A_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS5016248.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1135..1827)
                     /locus_tag="ACG81A_002984"
     CDS             complement(1135..1827)
                     /locus_tag="ACG81A_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS5016249.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          531 a          369 c          297 g          740 t
ORIGIN      
        1 cttttaaata cttaattgtt aaatacctta tttgatgcaa aagaaatcta cgatagtcaa
       61 caaaaaaatt ttcattatat ccagaaaaat ttttcttagt taaggaccta tggattgcag
      121 taatgcagaa ttcgtcgaat aaactgccaa agacatacaa tcatattgct tttaaaattg
      181 acgaacaaga tatagattcg tttgtctcaa aaatccaaat gttaggttta actgtcgaac
      241 caggacgttc tcgtgtgaaa ggcgaggctt cttcaattta cttttacgac tatgataatc
      301 acctcttcga gttacatacc ggtactctcc aggaacgttt aaaatcctac aattccacta
      361 cctgagacta gtaatgacta gtctcttttt atggcctctg atccccccat tgagcgaata
      421 ctaatagggc ttcgaattga tctgttcaat ctttaagcaa tatccctcaa atgagtttgg
      481 aaagtttagt gattcatcac aaatacttaa atacatgtag ttatatatag attgaggttc
      541 ttgtgagcgt atgctcgtgt gggttcaaat cccatcgtct gcatccatct ttccaaaaca
      601 agcggctagg acatttttgt ctcagccgct tgttttctag tttattcttt catcttgtcg
      661 gacctttttt cttgggtata gacttttctc atattctacc acaattttgg gtttccctaa
      721 cacatgatag gatctcacaa caaattgctt tctattatta taataacctg taacgactaa
      781 cttcatccct tcgtctactt cagctaggaa gttcaaacta tgggaagcaa tcaaacaatt
      841 gaccccattt aaactgaaac ggatcaacgg tctggcacta acttgcaaag tacggatctt
      901 atctactgtt ccatattttt tctccataac cttcacctca ataccattat acgaacatac
      961 attccctttg taaagaagaa actctgtttt atctttttga ctttttgtct attacctttc
     1021 ttaaaatcat attggcactt cctgcgaaga tgaaggacat gtactaaaaa aaagaggcag
     1081 cagtatagat tactgttgct tctttactaa tttaggttta cttgttaaga aagattattt
     1141 acgcaaatgt tcttcaacaa tttgctgaga tttatcagct gtatcttcca cttcatcttt
     1201 tgctttttct aacttttgaa tatattgttt caagcttgag ttttcagcag ttgcttcgtc
     1261 taatttcttt tgtgtatctg ttaactgagt ttctacattt gttttttctt gtgtcagtgt
     1321 tgcgatttgt tgattcaaac catttacagt attttgtaat tctgaaagtt ttgcttgtgc
     1381 ttcttgtagt cttgctgtcc aatctgcatt cagatgattg atttcatcat ttttttggtt
     1441 gatctcatct agtttgacat tgatttcatt ttgtttgctt tgtagttgtg catccttgct
     1501 gactaattga ctttctaagt tttgtttttc tgctgccgat tgattttttt ggttttctaa
     1561 ttcatttact tttgtttggt attcttggac atctgccttt gaatcttgca atgtctgttc
     1621 tttttgtgct agtcgagcgg taagatctgt aagcgcttgc tctttatctg tcaatgtatg
     1681 atcaagctgt gtcagattct cattgattgt atcaatatct gtctctccac cccacaaaat
     1741 aatttcattt gcgtaccgcg tgcctgtgta gccaattaga catagaaatg caacgacaat
     1801 cccaccagta ataaatttct ttttcatttg catagctcct taatccaata cttttacatg
     1861 ctaaaagtaa gtgatctcaa acacaatgtc aagcgttttt aaattacatg tgtaatttta
     1921 atataaatac tatcaaa
//
LOCUS       DAZULY010000485         1504 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.guided.342, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000485 DAZULY010000000
VERSION     DAZULY010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.guided.342"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            262..345
                     /locus_tag="ACG81A_002985"
                     /pseudo
     CDS             262..345
                     /locus_tag="ACG81A_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
     gene            394..477
                     /locus_tag="ACG81A_002986"
     CDS             394..477
                     /locus_tag="ACG81A_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HFS5016250.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="ACG81A_002987"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="ACG81A_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFS5016251.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAZULY010000486         1397 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.guided.354, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000486 DAZULY010000000
VERSION     DAZULY010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.guided.354"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..1270
                     /locus_tag="ACG81A_002988"
                     /pseudo
     CDS             143..1270
                     /locus_tag="ACG81A_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
BASE COUNT          468 a          204 c          267 g          458 t
ORIGIN      
        1 tcacaaattt tcattcaatt atcattttca attgcatttt ataaatcatt ttgtatgtaa
       61 tttggtatgt aagttaaaat gaaaaaagta gtccattttg aagtacattt tgtcattttg
      121 tactccaaaa ctgaactact tacttgcata tatacgcctt tatagtggag tactacattt
      181 acgagattcg gttagagtat cagaaaaaga aaaaataaaa gttacagaaa tgtatacttc
      241 aataaatggt gaattatgta agattgatag agcttattct ggagaaattg ttattttgca
      301 aaatgagttt ttgaagttaa atagtgttct tggagataca aaactattgc cacagagaaa
      361 aaagattgaa aatccgcacc ctctactaca aacaactgtt gaaccgagta aacctgaaca
      421 gagagaaatg ttgcttgatg cccttttgga aatctcagat agtgatccgc ttctacgata
      481 ttacgtggat tctacgacac atgaaattat actttctttc ttagggaaag tacaaatgga
      541 agtgattagt gcactgttgc aagaaaagta tcatgtggag atagaactaa aagagcctac
      601 agtcatttat atggagagac cgttaaaaaa tgcagaatat accattcaca tcgaagtgcc
      661 gccaaatcct ttctgggctt ccattggttt atctgtatca ccgcttccgt tgggaagtgg
      721 aatgcagtat gagagctcgg tttctcttgg atacttaaat caatcatttc aaaatgcagt
      781 tatggaaggg atacgctatg gttgcgaaca aggattatat ggttggaatg tgacggattg
      841 taaaatctgt tttaagtacg gtttatacta tagccctgtt agtactccag cagattttcg
      901 gatgcttact cctattgtac tggagcaagc ctttagaaaa gctggaacag aattgttaga
      961 gccatatctt agttttaaag tttatgcacc acaggaatat ctttcacggg catataacga
     1021 tgctcccaaa tattgtgcaa atatcgtaaa tactcaactg aaaaataatg aggtcattat
     1081 tattggagaa attcctgctc gatgtattca agattatcgc aatgatttaa ctttttttac
     1141 aaatgggctt agtgtttgtt tagcagagct aaaaggatat caggttacca ctggcgaacc
     1201 tgtttgccag acccgtcgtc taaatagtcg gatagataaa gtaagatata tgttcaataa
     1261 aataacttag tgcgttttat gttgttatat aaatatggtt tcttattaaa taagatgaaa
     1321 tattctttaa tatagatttg aattaaagtg gaaaggagga gattgttatt ataaactaca
     1381 agtggatatt gtgtcct
//
LOCUS       DAZULY010000487         1358 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.guided.358, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000487 DAZULY010000000
VERSION     DAZULY010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.guided.358"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1350
                     /locus_tag="ACG81A_002989"
     CDS             <1..1350
                     /locus_tag="ACG81A_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HFS5016252.1"
                     /translation="RDFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQ
                     IYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANA
                     VILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMK
                     YLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLN
                     TNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFL
                     RQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVF
                     EGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFG
                     EDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          571 a          107 c          230 g          450 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaaccg aatcattaaa aaaacattat
       61 acagagcctt gggaagatga agtttttaga gtaattattg aatataacaa tgttcctatt
      121 ggatatggac aaatatataa aatgtatgat gagttatata ctgattatca ttatccaaaa
      181 actgatgaga tagtctatgg tatggatcaa tttataggag agccaaatta ttggagtaaa
      241 ggaattggta caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct
      301 aatgcagtta ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa
      361 tctggtttta gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa
      421 gattgttatt taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa
      481 tatttaattg agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt
      541 ggttatgata gtgtggcata tttagttaat aatgaataca tttttaaaac aaaatttagt
      601 actaataaga aaaaaggtta tgcaaaagaa aaagcaatat ataatttttt aaatacaaat
      661 ttagaaacta atgtaaaaat tcctaatatt gaatattcgt atattagtga tgaattatct
      721 atactaggtt ataaagaaat taaaggaact tttttaacac cagaaattta ttctactatg
      781 tcagaagaag aacaaaattt gttaaaacga gatattgcca gttttttaag acaaatgcac
      841 ggtttagatt atacagatat tagtgaatgt actattgata ataaacaaaa tgtattagaa
      901 gagtatatat tgttgcgtga aactatttat aatgatttaa ctgatataga aaaagattat
      961 atagaaagtt ttatggaaag actaaatgca acaacagttt ttgagggtaa aaagtgttta
     1021 tgccataatg attttagttg taatcatcta ttgttagatg gcaataatag attaactgga
     1081 ataattgatt ttggagattc tggaattata gatgaatatt gtgattttat atacttactt
     1141 gaagatagtg aagaagaaat aggaacaaat tttggagaag atatattaag aatgtatgga
     1201 aatatagata ttgagaaagc aaaagaatat caagatatag ttgaagaata ttatcctatt
     1261 gaaactattg tttatggaat taaaaatatt aaacaggaat ttatcgaaaa tggtagaaaa
     1321 gaaatttata aaaggactta taaagattga ttatataa
//
LOCUS       DAZULY010000488         1350 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.guided.360, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000488 DAZULY010000000
VERSION     DAZULY010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.guided.360"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..298
                     /locus_tag="ACG81A_002990"
                     /pseudo
     CDS             47..298
                     /locus_tag="ACG81A_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="ACG81A_002991"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="ACG81A_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFS5016253.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DAZULY010000489          990 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.guided.401, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000489 DAZULY010000000
VERSION     DAZULY010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.guided.401"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            170..952
                     /locus_tag="ACG81A_002992"
     CDS             170..952
                     /locus_tag="ACG81A_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HFS5016254.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
BASE COUNT          348 a          140 c          204 g          298 t
ORIGIN      
        1 gtataataaa gaataattat taatctgtag acaaattgtg aaaggatgta cttaaacgct
       61 aacggtcagc tttattgaac agtcatttag gtatatgtcc aatctagggt aagtaaattg
      121 agtatcaata taaactttat atgaacataa tcagttacga ggtgaaatca tgagcaattt
      181 gattaacgga aaaataccaa atcaagcgat tcaaacatta aaaatcgtaa aagatttatt
      241 tggaagttca atagttggag tatatctatt tggttcagca gtaaatggtg gtttacgcat
      301 taacagcgat gtagatgttc tagtcgtcgt gaatcatagt ttacctcaat taactcgaaa
      361 aaaactaaca gaaagactaa tgactatatc aggaaagatt ggaaatacgg attctgttag
      421 accacttgaa gttacggtta taaataggag tgaagttgtc ccttggcaat atcctccaaa
      481 aagagaattt atatacggtg agtggctcag gggtgaattt gagaatggac aaattcagga
      541 accaagctat gatcctgatt tggctattgt tttagcacaa gcaagaaaga atagtatttc
      601 tctatttggt cctgattctt caagtatact tgtctccgta cctttgacag atattcgaag
      661 agcaattaag gattctttgc cagaactaat tgaggggata aaaggtgatg agcgtaatgt
      721 aattttaacc ctagctcgaa tgtggcaaac agtgactact ggtgaaatta cctcgaaaga
      781 tgtcgctgca gaatgggcta tacctctttt acctaaagag catgtaactt tactggatat
      841 agctagaaaa ggctatcggg gagagtgtga tgataagtgg gaaggactat attcaaaggt
      901 gaaagcactc gttaagtata tgaaaaattc tatagaaact tctctcaatt aggctaattt
      961 tattgcaata acaggtgctt acttttaaaa
//
LOCUS       DAZULY010000490          855 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5119
            SAMEA115707506-rid22580373.guided.416, whole genome shotgun
            sequence.
ACCESSION   DAZULY010000490 DAZULY010000000
VERSION     DAZULY010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115707506
            Sequence Read Archive: ERR13197235
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002288047.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002288047.1
            Genome Coverage       :: 187x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 11:09:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,993
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 49 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115707506-rid22580373.guided.416"
                     /isolate="VRE5119"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..688
                     /gene="aac(6')"
                     /locus_tag="ACG81A_002993"
     CDS             140..688
                     /gene="aac(6')"
                     /locus_tag="ACG81A_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFS5016255.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          306 a          126 c          189 g          234 t
ORIGIN      
        1 tgtataattt ttttatttta taagtaagat aaataatgaa agaatagaga tactgattgg
       61 tagctaaaga tttttgagcg agaacatttc gttttttcaa aaggactgtt agaataagca
      121 agaacaaggg ggaagacgta tgataatcag tgaatttgac cgtaataatc cagtattgaa
      181 agatcagctt tctgatttac tgagactgac ttggccggaa gaatatggag acagctcggc
      241 agaagaagta gaagaaatga tgaatccaga acgaatcgcg gtagcagcgg tagaccaaga
      301 tgagttagta ggatttattg gtgcaatccc tcaatacggt atcacaggtt gggaattgca
      361 tccattagtt gtagaaagct cccgacgaaa gaaccaaata ggtactcgat tagtcaatta
      421 cttagaaaaa gaagtagctt ccagaggagg aatcacgatt tatttaggta cggatgattt
      481 agaccatgga acaacgttaa gtcaaacgga cctgtatgag catacatttg ataaagtggc
      541 ttccatccag aaccttcgtg aacatccgta tgaattctat gaaaaattag gttataaaat
      601 cgtaggtgtc ttaccaaatg caaatggctg ggacaaaccg gatatttgga tggcaaaaac
      661 gattattcct cgaccaaatt ctcaataata aaaagaggtt gtgacagaag taatcagctt
      721 caagaaataa gaaggaattt acgaaaatta cttcttgaat ttttgtgaaa ttccaactta
      781 tttccgaaga agctactttt ggaacaccgt gtaccagagg ttgtgaaaaa gtcgcttagt
      841 ttcgagtagt aagaa
//
